LOCUS       DAOOMA010000001        17906 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_3259_length_17906_cov_4.764383, whole genome shotgun sequence.
ACCESSION   DAOOMA010000001 DAOOMA010000000
VERSION     DAOOMA010000001.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 17906)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17906)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17906
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3259_length_17906_cov_4.764383"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..946
                     /locus_tag="PLP95_00005"
     CDS             <1..946
                     /locus_tag="PLP95_00005"
                     /inference="COORDINATES: protein motif:HMM:NF015734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PASTA domain-containing protein"
                     /protein_id="HPB44217.1"
                     /translation="SVVVLLLVLGGLITWVVWSSRSDVEEVAVPNVATRTEQEAVELL
                     KAAGFNPVIQREASNDVLEDIVIRTDPAGETMAEKGSDVTLFVSSGPDGIEVPKIIGL
                     PVAEAQAALTAAELKSNVTDVANDAAVGTVVAATPTEGELVQPGALIELQVSAGPAPV
                     DIPDVSNRTVNEARRALSDAGFVVGTTTTEPSNDVAKDRVIRTDPAGQAAKNATINLV
                     VSEGRAPVKVPDVVGSLEADARAEITALGLRVSSRTRQLPAGDANNGRVVEVSPSEGT
                     DVGADSTVTITIGVASAAATTTTSSTPATTSTTVAGSP"
     gene            1030..2088
                     /locus_tag="PLP95_00010"
     CDS             1030..2088
                     /locus_tag="PLP95_00010"
                     /inference="COORDINATES: protein motif:HMM:NF015734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PASTA domain-containing protein"
                     /protein_id="HPB44218.1"
                     /translation="MNRTSNDLPGRTGTVAVVGALAFALLSTVLFTFSPSLNPFDLLA
                     GRFLEVAVPDVRGLTQQKALVQLTRDRLAGEVEFAYDAEVPVGSVARQEPAPSETVKR
                     GGIVVLTVSRGPAFVPVPDIIGAKRGDAVTQLKSLDLEVVEVEQLSEEIDADRTISVV
                     PSVGTIVQSGQKVTLTVSTGPEIRAVPAVPGMTLNGAAFLLGRAGLAIGTVTYQDSLD
                     VRSGAVIATDPAVGVELERDAPVSLVVSSGKPAGPVPSVLGRAQADAVKTLTAAGFVV
                     GEVSELVPFGDPGTGTVSSQVPASGTVLRPGEVVTITVKRALPPPTTVPLTTQPPTTT
                     TTTTTIPVAPVETIPGGP"
     gene            2085..3086
                     /locus_tag="PLP95_00015"
     CDS             2085..3086
                     /locus_tag="PLP95_00015"
                     /inference="COORDINATES: protein motif:HMM:NF015734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PASTA domain-containing protein"
                     /protein_id="HPB44219.1"
                     /translation="MNSHVTVREVEPRSAVGRPEIVDERRTRLRFAAVLAAVVAAMAL
                     ALWGFTDSLVGAEDVQLPRLAVPSVEALSLADATARLEDTGFTVTVLYQPNEDKPKGE
                     VIGQRPTAGYKADQGDLITILVSDGPLGWTVPSIVGLQVEDALISLGGVEVALEINDV
                     HDETIPDGEVIESMPSEGSRVGPTTKLRLTVSAGPAPRIVPEIVNQPIEQGLVAIGRA
                     GLMVGKITEVARPELAPGTIVELGVAPGTGLARDTPVDITVSVAQDMRPVPYLTGLLR
                     SSAEEMIRDAGLVAQVVTSEVPPGSSLAGRVTVQGTAPAVRLPRGSVVSITVGVAAP"
     gene            3132..3698
                     /locus_tag="PLP95_00020"
     CDS             3132..3698
                     /locus_tag="PLP95_00020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB44220.1"
                     /translation="MKIESSGGFTIAESAVALGSLRYWELELHRLLGSAVPHIENHEL
                     KLLCADLSEHHAWHGEMLAERMPTVRDLSPAAQTVGTRAAVSVVEALDDADDISRAAW
                     FGAYARWVLPQLLGSYVAYRAGRSPVADAAVIRTVGFVIDDLSADLATASMVASSATQ
                     DSDDLRAGEGLVTRLNAHRLEFGPLTAT"
     gene            3904..4842
                     /locus_tag="PLP95_00025"
     CDS             3904..4842
                     /locus_tag="PLP95_00025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015439938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HPB44221.1"
                     /translation="MAKDRIDRDEEDLVRLYLTDIGQYPLLTKDDEVRLAKAIEQGTA
                     AAAELEDPTVKVTAAKRRELRRAVRSGQEAERAFVQSNLRLVVSIAKKYQASGLPLLD
                     LIQEGNLGLMHAVEKFDWRKGFKFSTYATWWIRQAITRGIANTGRTIRLPVHAGDTLT
                     RVQKARARLELKYGRPATLAELAAEVELPEDKVTEALRFASEPLSLSEPLREDGDAEL
                     GDVVEDRGATSPFDAAATSMLPEEVSRLLSPLDDREREILKLRYGLDRGEARTLEDVG
                     EHFNLTRERIRQIEARAMSKLRHPSSDTGARDLLEV"
     gene            5088..5672
                     /locus_tag="PLP95_00030"
     CDS             5088..5672
                     /locus_tag="PLP95_00030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB44222.1"
                     /translation="MDGRCLLIDLDGDLPAAAGIAEPMVGVTDWLASSSGSPEALERL
                     ATRVSDVGVELVGIGSSHKWERSRADDLIDALSSTQTFVVVDVGCVDSVRTPIEPIRR
                     KLAALPTSVFVTRACYLALHRAVQLDLSIGRVALIRENGRSLDRLDVSRLLAASEVVD
                     VDVHPAVARAIDSGSMVRRVPRLLNRQLADLVAP"
     gene            5669..6742
                     /locus_tag="PLP95_00035"
     CDS             5669..6742
                     /locus_tag="PLP95_00035"
                     /inference="COORDINATES: protein motif:HMM:NF012652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HPB44223.1"
                     /translation="MIPLDDAWVRECVAQSPGEAIDTQLLRLVRQQAPLATDDAVRGE
                     VERLRSTLEGVGPLLELLADPDVSDVLINGPGPAWVERSGRLVPTSVSLTSTDIDRLL
                     QGVFHRTGRSLDRSHPIGDARLRDGTRISAVLSPLAVHGPVVALRRRVERAITLESFS
                     VATITTAVEELVAARRNVLVFGATGAGKTTLLAEMASLIPHSERVFTIEDAAELCIDH
                     PHVVALECRPANSEGRGAVTLRDLVAASLRLRPDRIVLGEVRGAEALDVVWALASGHR
                     GSLATCHAASAAGALQRLETFVAMGDAGLPHHVIRAQVRSAFDFLIGVERHGENRRIS
                     SLHRVVDDHSDAAGVEAIWQVDR"
     gene            6739..7488
                     /locus_tag="PLP95_00040"
     CDS             6739..7488
                     /locus_tag="PLP95_00040"
                     /inference="COORDINATES: protein motif:HMM:NF012694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HPB44224.1"
                     /translation="MMEHLRGPALLITVSVLVIAVRDALRAQRRPRRQPVAAGLPHEL
                     HPGSLAGLPSVSPRRVATRSAGLRWLPGAGRRRRRAELAALIDVCEQLAIALAAEGTL
                     RAALMAVPSPTCARLRHGLESGRTVGDAVQHWVDEAPRERRMVAAVLEAVADGGADGV
                     RALDRCASVLRSRTELDDELVSASAQARMSALMMTLLPVAGVAALGAIEPGTLAFLFT
                     TSTGVVLAAGATAWNACGWLWMRSMIAGAGR"
     gene            7485..8183
                     /locus_tag="PLP95_00045"
     CDS             7485..8183
                     /locus_tag="PLP95_00045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB44225.1"
                     /translation="MTHLGALGAASVVTLAWVLVVVEGVRRVRVRRQSTVERLSPLPV
                     ASGREPAHAGSRVRRVLVERPARSVVSDIGPRLRTSARRRRRELEELDALSEWIDLVA
                     LSCAAGSTVATAVERATTAMSWAAPLGRELRRQPSIDRALDVWGAEGGDDRRALSALI
                     NVSLVSGSALSERLSNRSDSLRSRRRHEALAAARRLPVRLLVPLVVCALPSFVALSVV
                     PLLVVSLQSLSWEP"
     gene            8248..8481
                     /locus_tag="PLP95_00050"
     CDS             8248..8481
                     /locus_tag="PLP95_00050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB44226.1"
                     /translation="MLNQPHCSASWSRRLRCVAWRAATDQRGQSTVEYALILVAVTVM
                     VGVVIAYLAGDGGGTITALFSAAFARMRNLVGG"
     gene            8519..8887
                     /locus_tag="PLP95_00055"
     CDS             8519..8887
                     /locus_tag="PLP95_00055"
                     /inference="COORDINATES: protein motif:HMM:NF019428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein"
                     /protein_id="HPB44227.1"
                     /translation="MTRARRGQRGQATVEMALVLPVLILGILIVLQILLVARDAASLT
                     AATRAAARAAMVDDDPVEAALHERHLDRSRLRVEVSGTAAAGELITVSATYRSPTGLA
                     VVGRFVGDVTLTERFTVRKE"
     gene            8962..9309
                     /locus_tag="PLP95_00060"
     CDS             8962..9309
                     /locus_tag="PLP95_00060"
                     /inference="COORDINATES: protein motif:HMM:TIGR00377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HPB44228.1"
                     /translation="MHFRVRSVDRGRWTSVLVTGDVDLAALPLYRRELHDAVAGAKPL
                     EIDLSECDFMDSTGLGVTIGAVRRVWDRDGEVRIVTSPPVQRLMTRCRLDEILPLTAP
                     SVQSADPGEVLAP"
     gene            9306..9764
                     /locus_tag="PLP95_00065"
     CDS             9306..9764
                     /locus_tag="PLP95_00065"
                     /inference="COORDINATES: protein motif:HMM:NF024970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPB44229.1"
                     /translation="MTDEQFVELMIPAESSFVATARRFITDVAGKADWMDQQHLDDLR
                     LMASEAVTNALRAQRRAEVGDKICVRCMLFSDRFEFSVADSAGGFERPAHLDIHTDPE
                     LSREGGFGLPLIEALADEAHFVNTDGGTTVRLVVYRPGCSSTRESTSTTR"
     gene            complement(9713..12091)
                     /locus_tag="PLP95_00070"
     CDS             complement(9713..12091)
                     /locus_tag="PLP95_00070"
                     /inference="COORDINATES: protein motif:HMM:NF012905.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HPB44230.1"
                     /translation="MDAMSQRITGLSSTEVSERIAAGLVNEVPPAPSRTIPEIIRANV
                     FTRFNALIALLAAIVIAAGSPIDAMFSGVIVANIFIGIFQEVRAKRTLDRLALLNAPH
                     AHLLRDGEVRDHPVNDIVLDDVIELRPGGQIVADCVLVDCDGLEIDESMLTGESDAVV
                     KGTGDELLSGSFVVTGSGYARVTKVGKDAYAARLAEEARRFTLANSELRASIEQIVTW
                     VSWALIPAAIGLVWSQMRSNDDIRAALVAMVAGLVGMVPEGLLLLTSVAFFVGVVRLA
                     RRRTLVQELPAVEILARVDVVCLDKTGTITEGTMVVDSVTALGGHDDELGSLALTAIA
                     HSDPNPNATQQALIERFSEPLASGWERLDGVPFSSARKWSANRFEGQGWWVLGAAEMI
                     LDDAKLAGVSEQIEAASGNGLRILLLAHTDSPVSSDRALPGDIVAVALVSLIDKIRPD
                     AAETLRYFADQGVTLKVISGDNPTTVGAVAERAGLEVDNHLVDARTLPDSDGTDDEFA
                     DLVEAGTVFGRVTPHQKRAMVTALQRRDHIVAMTGDGVNDVLALKDADCGIAMASGSE
                     ATRAVAQVVLLDSSFSGLPAVVAEGRRVINNLQRVASLYLTKTTYAVIFAFVTGIFAM
                     DFPFLPRHLTLISSLCIGIPSFFLALAPNTARVQGNFLRRVGWDSVPMGILCAAATMA
                     AFVIMRSTTGTTLEEQQTAATLTLAGCSLLVLVRNARPIRGWKLGLIATMGLGVVAAF
                     TTPLGKEYFQLTLPAAESVWICLAIVGLTGLAMSIVHRVVDVDSRVLEHPGR"
     gene            complement(12095..13300)
                     /locus_tag="PLP95_00075"
     CDS             complement(12095..13300)
                     /locus_tag="PLP95_00075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HPB44231.1"
                     /translation="MDFQLSPTATALQERLLEFMDDKVYPAEATYHDQMANLDPHDHP
                     PVIEDLKAEAKARGLWNLFLPHKTQWTDGLSNLDYAPLAEITGRSMHLAPEALNCSAP
                     DTGNMELLTMFGTDAQKEEWLQPLLNGEIRSAFAMTEPDVASSDATNIELSITRDGDS
                     YVLNGHKWWTSGAMSPRCTLFIVMGKTDPTAAKHLQQSMILVPRDTRGVTVVRNLEVF
                     GYTDREGHAEVHFDNVRVPATNLLHTEGSGFMMAQARLGPGRIHHCMRAIGIAERALE
                     LMCRRVTQRVAFGRPLAEQGVIREWIADSRMEIEQARLLTLKAAWLMDTVGNRGAAIE
                     ISSIKVVAPNVALRVVDRAIQAHGGGGVSSDFPLAAMYAGLRTLRLADGPDEVHRRQV
                     ARTELGKYS"
     gene            13437..16235
                     /gene="topA"
                     /locus_tag="PLP95_00080"
     CDS             13437..16235
                     /gene="topA"
                     /locus_tag="PLP95_00080"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020378945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HPB44232.1"
                     /translation="MGQPLVIVESPAKARTISRFLGDEYVVESSVGHIRDLPSKAAEV
                     PAAYKGTVLAKLGVDPDDGFKPLYIVSPNKKEQVKRLKDLLKDADELYLATDEDREGE
                     AIAWHLLEVLKPKVPVRRMVFHEITPDAIQSAIENPRDLNRSLVDAQEARRIFDRLYG
                     YEVSPVLWKKVMPRLSAGRVQSVATRIVVERERERMRFRTASYWDLSATFQLTGAGKD
                     TTPFGANLIEVDSLRVATGRDFDETGSLTRDGAVLLDEESAGALRAALEGRPATIASV
                     ERKPYTRKPYAPFMTSTLQQEAGRKLRFSSSQTMSLAQRLYENGFITYMRTDSTTLSE
                     TAINAARSLIASKYGEAYLPDSPRVYAKKSKNAQEAHEAIRPAGDTFRSPEEVRSVLG
                     TQEFRLYELIWQRTIASQMTDARGESMQVRIDVDASDGRRARFAASGKTILFPGFLRA
                     YVEGADDPDAELEDQERILPDMAEGDGARSTELDAKGHETQPPARYTEASLVKRLEEL
                     GIGRPSTYASTISTIQDRGYVRKKGSALVPSFTAFAVVTLLEQHFPDLVDYALTAHME
                     DDLDRIATGDASSAPWLSSFYFGDGVDSDRPGLKHMVTDIEHIDARAVNSIPIGADAN
                     GELIVARVGRYGAYIQRGDDTANIPDDHAPDELTPDKAVELLNTPKERKLGDDPATGK
                     PIYVKNGRFGPYVQLGDHDDETGEKPKMASLFQTMTLERVSLDDALELLSLPRVLGVD
                     PADGVEITAQNGKFGPYIKKDTDSRTIENEEMLLTITLDEALRILAEPKRRRGQAVKG
                     PLKDLGKDPDSERPILCKDGRFGPYVTDGITNASLRKGDSVEELTIERAMELLADRRE
                     KDIAAGKDPAKKASARKAPAKKAGAKKTAAKKAPAKKTAAKKAPAKKAAAKKSPTAAV
                     TPKAVADAFIAADGDEPF"
     gene            16235..16900
                     /gene="tmk"
                     /locus_tag="PLP95_00085"
     CDS             16235..16900
                     /gene="tmk"
                     /locus_tag="PLP95_00085"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HPB44233.1"
                     /translation="MSSTAAGGTGRVDRTAPFIVFEGGEGGGKTTQAALLRERLADRV
                     GIEAIVTRQPGGTAIGARLRSILLDVDNAELSDRCEALLMAADRAQHATELIGPTLAS
                     GVGVICDRYLYSSVAYQGYGRGLDAQKVAEISEWATAALLPDLVVLIDVDLDTASGRL
                     QRELDRFEQAGADFHERVRRGFLAQANEDPSRWVVIDGSRSIDEVAEQIWDAVARLPM
                     VTQ"
     gene            16897..>17906
                     /locus_tag="PLP95_00090"
     CDS             16897..>17906
                     /locus_tag="PLP95_00090"
                     /inference="COORDINATES: protein motif:HMM:NF024575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB44234.1"
                     /translation="MTTGMEATSDDLDDLWSEVVGQDQLVAQLRRAAADPTHAYLFVG
                     AAGVGTRAAARAFAAELLSGGADSVDIARHCALAKAEHHPAIRFVERTGASISADQAR
                     EIVRQAQMRPPEGDLQVIVLVDFHLVSTAAPILLKSIEEPPSGTVFIVLADEVPPDLV
                     TIASRCVTFEFPILSTALIAECLRRDGVSGDVADAAAAGAAGNIDRARLLATDPLVVA
                     RRQFWIDLPGRLDGTGATAARLASEVIGRVDEVLEPLAELHSRELTKLAEQAERLGLR
                     KSQQAEVEARHKREQRRVRSDELRAGLAALMEGYRRDAATAPDAFVAAAELVGEFTER
                     LV"
BASE COUNT         3001 a         5566 c         6221 g         3118 t
ORIGIN      
        1 gtcggtcgtg gtgttgttgt tggtgctcgg tgggctcatc acctgggtcg tgtggtcctc
       61 gcgatccgac gtggaggaag tcgcggttcc gaatgtcgcg accaggaccg aacaggaagc
      121 ggtcgagctg ttgaaagcgg cggggttcaa cccggtcatt caacgcgaag cctcaaacga
      181 cgttctcgaa gacatcgtca ttcgcaccga ccccgctggc gaaacgatgg cggagaaggg
      241 ttcggacgtc accttgttcg tgagtagcgg acccgatggc atcgaggtgc ccaagatcat
      301 cgggctgccg gtggcggagg cgcaggcggc attgacggcg gcggagttga aatccaacgt
      361 caccgacgtc gcgaacgatg ccgccgtcgg aaccgtggtg gcagcgactc cgaccgaagg
      421 cgaacttgtg cagccgggag cgttgatcga attgcaggtc agcgccggtc ccgcccccgt
      481 cgacatcccc gacgtttcga accggaccgt caacgaggcg cgtcgggcgc tcagcgatgc
      541 cgggttcgtc gtcggcacca cgaccaccga gccatccaac gacgtcgcca aggaccgggt
      601 gattcgcacc gaccctgctg ggcaggccgc caagaacgcc acgatcaacc tcgtggtttc
      661 cgaggggcgg gcacctgtga aggtccccga tgtggtcggt tcgctcgagg ccgacgcccg
      721 cgctgaaatc actgcactcg ggttgcgagt gtcgtcgagg acgcggcaac ttcctgctgg
      781 tgacgcgaac aatgggcgtg tcgttgaggt gtcgccgagc gaaggaaccg atgtcggcgc
      841 cgacagcacc gtgacgatca cgatcggcgt cgccagcgcg gcggcgacca cgaccacgag
      901 ttccaccccg gcgacgacct cgaccacggt ggccgggtct ccgtgatcga ggggagcggt
      961 tcgcggcgtc gccgcgaccc gcggcccgac tgttcgtggg tgtgacgacc gagctggtga
     1021 agcgaattga tgaaccgaac gtcgaatgat ctcccgggcc ggaccggaac ggtcgcggtc
     1081 gtcggggcgc tcgcattcgc gttgttgagc accgtgttgt tcacgttttc tccgagcttg
     1141 aatccattcg atctgctggc gggtcggttt ctcgaagtcg ccgtgcccga cgtgcgaggc
     1201 ctcacccaac aaaaggcgtt ggtgcaactc acccgtgatc gactcgcagg tgaggtggag
     1261 ttcgcgtacg acgcggaggt cccggtcggc agtgtggctc gtcaggagcc ggcgccctcc
     1321 gagaccgtca aacggggcgg aatcgtggtt ctcaccgtga gtcgcgggcc ggcgtttgtt
     1381 ccggtgccgg acatcatcgg agcgaagcga ggcgatgcgg tgacgcaact caagagcctt
     1441 gatctcgagg tggtcgaggt ggagcagctg agtgaggaga tcgacgccga tcggacgatt
     1501 tcggtggtac cgagtgtcgg gacgatcgtg cagagcggcc agaaagtgac actgacggtg
     1561 tcgacgggtc cggagattcg tgcggtgccc gcagttcccg gcatgaccct caatggtgcg
     1621 gcgttcctct tggggcgcgc cgggcttgcg atcggaaccg tgacctatca ggacagtctt
     1681 gatgttcgtt cgggggcggt catcgcaacc gacccggcgg tcggtgtgga actcgaacgg
     1741 gatgcgccgg tcagtctcgt ggtttcgagc ggcaaaccgg cgggtccggt gccgagcgtg
     1801 ctgggccgcg cccaggccga cgcggtcaag acgctgaccg cggctgggtt cgtcgtcggt
     1861 gaagtgagcg aactcgtccc gttcggcgat ccaggtaccg gcaccgtcag tagtcaggtg
     1921 ccggcttcgg gcacggtgtt gcgcccgggg gaggtggtga cgatcacggt gaagcgagcg
     1981 ctaccgcctc cgacgacggt gccgctgacc acccaacctc cgacgaccac gaccacgacc
     2041 acgaccattc ccgtcgcacc cgtcgaaacg attccggggg gcccatgaac tctcacgtga
     2101 cggtacgcga agttgagccc cgttcggccg tcggccggcc tgagatcgtc gatgagcgcc
     2161 gtacgcgact gcggtttgcc gcggtattgg cagcggtcgt cgcggcgatg gccctggcgc
     2221 tgtggggatt caccgactcc ctggtcggcg ctgaggacgt ccagctccca cggctcgccg
     2281 tgccgtcggt cgaagcgctt tcgttggctg atgcaacggc cagactcgaa gacaccggct
     2341 tcacagtgac ggtgttgtat cagcccaacg aggacaagcc caaaggcgag gtcatcggcc
     2401 aacgcccgac cgccgggtac aaagcggatc agggggacct catcaccatc ttggtgagcg
     2461 acggaccact cggttggacg gttccgtcca tcgtcgggct tcaggtcgaa gatgcgttga
     2521 tctcgctcgg cggagtcgag gtcgcgttgg aaatcaacga cgtgcatgac gagacgatcc
     2581 ccgacggtga ggtcatcgag tcgatgccgt cagagggctc gcgggtcgga cccaccacga
     2641 aactccgcct caccgtgtcg gccggacccg ctccgcgaat cgtgcccgag atcgtcaatc
     2701 agccgatcga acaggggctg gtggcgatcg gacgagccgg gttgatggtt ggaaaaatca
     2761 ccgaggtcgc acgtcctgaa ctcgctccgg gaacgatcgt cgagttgggt gtagctccgg
     2821 gcacggggct ggcgcgcgac accccggtgg atatcacggt gtcggtagct caggacatgc
     2881 gaccggtgcc gtacctcacg gggctgttgc gcagctccgc cgaggagatg attcgcgacg
     2941 cgggactcgt tgcgcaggtc gtcacgagcg aagttccgcc ggggagttca ctggctggac
     3001 gtgtcacggt ccaaggaacc gcgcccgccg ttcgtcttcc ccgaggctcg gtcgtgtcga
     3061 tcaccgtcgg ggtagcggcg ccgtagaggc ccggcactag tgtgggcagc gcgacgggtg
     3121 aaggagacac gttgaagatc gagtcgtctg gcggattcac gatcgccgag tcggcggtcg
     3181 cgttgggttc gttgcgctat tgggaactcg agttgcatcg cctgctcgga tccgcggtgc
     3241 cacatatcga gaatcacgag ttgaaactgc tgtgcgcgga tctgtccgaa caccacgcgt
     3301 ggcatggcga gatgcttgcg gaacgcatgc cgaccgtgcg cgatctgtcg ccggcagccc
     3361 aaacggtcgg gacgcgagca gcggtctcgg tggtggaagc gctcgacgat gccgatgaca
     3421 tctcgcgggc agcatggttc ggtgcgtacg cccgatgggt gctgccgcaa ctgctggggt
     3481 cctatgtcgc gtatcgggcc gggcggtcgc cggtggcgga cgctgcggtc attcgcacgg
     3541 tggggtttgt catcgacgac ctcagcgccg atctggcgac cgcctcgatg gtggcgtcgt
     3601 cggcgacgca ggattccgac gacctccgcg cgggtgaagg tctggtcacg aggttgaatg
     3661 cgcatcgcct cgagttcgga ccgctgacgg cgacctgagc cgatcatcgg cccctcaagg
     3721 aatgttcggc ggttgtcgaa ggttcttctt gttcatcgga acgactcgtt ggatgtcgaa
     3781 tggttcggcg tcgattcggg tgcgccggat tcgggccgcc acttgacgcg gttcgtatcg
     3841 tgaaagtgcg tttcgttcca ccgctccgac acggggcgga cgagtggaag gaagggactc
     3901 accgtggcga aggaccgtat cgatcgcgac gaagaagacc tggtgcggct gtatctgacc
     3961 gatatcggtc agtatccgtt gctcacgaaa gatgacgagg ttcgcctggc caaggcgatc
     4021 gaacagggga cggcggcggc cgccgaactc gaagacccaa ccgtcaaggt gaccgccgcg
     4081 aagcgccggg agttacggcg cgccgtgcgt tccggccagg aggccgaacg ggccttcgtc
     4141 cagtccaacc tgcgattggt ggtgtcgatc gccaagaagt atcaggcgtc gggtttgccg
     4201 ctgttggacc tcattcagga aggcaatctc gggctgatgc acgcggtcga gaaattcgac
     4261 tggcgcaagg ggttcaagtt ctcgacctat gccacgtggt ggattcgcca ggcgatcact
     4321 cgaggcatcg ccaatacggg gcggacgatt cgccttcccg tgcacgcggg cgacacgctg
     4381 acccgcgtac agaaggcacg agcacgcctc gaactgaaat acggccgacc cgcaaccctg
     4441 gcggagttgg cagctgaggt cgagctgccc gaggacaagg tgaccgaggc actgcgattc
     4501 gcctcggaac cgttgtcgtt gtcggagccg ttgcgcgaag acggcgatgc ggaactcggc
     4561 gatgtggtcg aggaccgcgg ggcgacctcg ccgttcgacg cggcggcgac ctcgatgctg
     4621 cccgaggagg tctcgcgttt gttgtctccg ctcgacgatc gcgaacgcga gattctcaag
     4681 ctgcgctacg gactcgaccg cggtgaggca cgaaccctcg aagacgtcgg ggagcatttc
     4741 aacctcaccc gcgagcggat tcgccagatc gaggcccgtg cgatgtcgaa gcttcgccac
     4801 ccgtcctcgg ataccggggc gcgcgacctg ctcgaggtct gatcgaggtc ggggcgcagt
     4861 gagcgcggct cggtcggtgc agcacggcgc ggccgcggag gctgggtagg ttcaccccct
     4921 tacggtctgg aattcgctcg ataggttcga gggcccttcc cctccggttc gtcacggcgc
     4981 gtgcgccaat tccggagttc ctcgacatgt tggttcccgt gtggtcggcg aaagccggcg
     5041 tgggctgtac caccctctca ctcgcccttg cggctcgcgt cgcgcgtttg gacggccgtt
     5101 gcttgttgat cgacctcgac ggcgatctcc ctgccgcggc ggggattgcc gagccgatgg
     5161 tcggcgtcac ggattggctc gcgagcagct cgggctcgcc cgaggcgctg gaacgtctgg
     5221 cgacgcgggt ctccgatgtc ggagtcgagc tcgtggggat cgggtcgtcg cacaaatggg
     5281 agcgttcccg cgccgacgac ctgatcgatg cactcagctc gactcagacg ttcgtcgttg
     5341 tcgacgtcgg ctgtgtcgac tcggtccgga ccccgatcga accgatacgt cgcaagcttg
     5401 cggcgctgcc gacgagcgtg tttgtgacca gagcgtgcta cctggcgctt catcgggcgg
     5461 ttcaactcga cctgtcgatc gggcgggtgg cgctcatccg ggagaacggg cgatccctcg
     5521 atcgactcga cgtctcgcgc ctgcttgcgg cgtcggaggt cgtcgatgtc gatgttcacc
     5581 cggcggtcgc ccgcgccatc gactccggct cgatggtgcg ccgggtcccg cggttgttga
     5641 atcgtcaact cgccgatctg gtggcgccat gatcccgctc gacgacgcat gggtgcgcga
     5701 atgtgtcgcg cagtcgccgg gggaggcgat cgacacccag cttctcaggc tggttcgaca
     5761 gcaggcacct ctcgccaccg acgatgcggt gcgcggcgag gtggagcgcc ttcgatccac
     5821 cctcgaaggt gtcggtccgc ttctcgagct cctcgcagac cccgacgtct ccgacgtgtt
     5881 gatcaacggt cccggtccgg catgggtgga acgttcgggt cgtctggttc ccacctcggt
     5941 gtcgctcacc tcgaccgaca tcgaccgact cctgcaaggg gtctttcacc gaaccggacg
     6001 aagcctcgat cgaagtcacc cgatcggcga tgcgcgccta cgagacggga cgcggatctc
     6061 ggcggtgctc agcccgctcg cggtgcatgg cccggtggtg gcattacgtc gccgagtgga
     6121 acgcgccatc acgctcgagt cgttcagcgt cgcgacgatc accaccgccg tggaagaact
     6181 cgtagctgcg cgccgcaacg ttctggtgtt cggcgctacc ggggccggca agacgacatt
     6241 gctggcggag atggcgtcgc tgatccccca ctcggagcgg gtgttcacca tcgaggacgc
     6301 cgccgagttg tgcatcgatc atccgcacgt ggtcgcgctc gagtgtcgac cggcgaattc
     6361 cgaagggcgc ggggcggtca cgttgcgcga tctggtggcg gcgtcacttc gattgcgacc
     6421 ggaccgcatc gtgttgggcg aggtgcgtgg tgccgaggcc ctcgacgtgg tgtgggcgct
     6481 ggcgtcgggc catcgaggat cgctggcgac ctgtcacgca gcatcggccg ccggcgcact
     6541 tcaacgcctc gaaaccttcg tggccatggg cgatgccggc ctgccacatc acgtcattcg
     6601 ggcgcaggtc cgctcggcgt tcgacttcct gatcggcgtt gaacggcacg gagagaaccg
     6661 gcgcatctcc tcgctgcatc gggtcgtcga tgaccactcc gacgctgccg gcgtcgaggc
     6721 gatctggcag gtcgaccgat gatggaacac ctccgaggtc cagcactgct catcaccgtg
     6781 tcggtcctcg tgatcgcagt acgagatgcg cttcgcgccc agcgccgacc tcgtcgccaa
     6841 cccgtggccg cgggcctacc tcatgaactg caccccggct cgctggcggg gctaccgtcg
     6901 gtctctcccc gccgcgtcgc aacgcggtcg gcggggctcc gatggttgcc aggcgcgggt
     6961 cgtcgtcgac gtcgagcgga actggcggca ttgatcgatg tgtgcgaaca gttggcgatc
     7021 gccttggccg ccgaggggac gctgcgtgcc gcactgatgg cggtcccgtc gccgacctgc
     7081 gcgaggctgc gtcacggcct cgagtcgggg cgcacggtcg gcgacgcggt tcagcactgg
     7141 gtcgatgagg caccacgaga gcggcgaatg gtggcagccg tactcgaagc ggtcgccgac
     7201 ggcggggcag acggcgttcg ggccctggat cggtgcgcgt cggtgttgag gtcgcgaacc
     7261 gaactcgacg acgagttggt gtcggcaagt gcccaggcga ggatgtcggc actgatgatg
     7321 accttgcttc ccgtcgcggg agtcgcggcg ctcggagcca ttgaaccggg aacccttgcg
     7381 ttcttgttca ccacctcaac cggggtcgtg ctcgctgccg gggcaaccgc ctggaacgca
     7441 tgcggttggc tctggatgcg ttcgatgatc gctggagcgg gacgatgacg caccttggcg
     7501 cgctcggggc cgcgtcggtg gtgacgctcg cgtgggtact ggtcgtcgtc gagggtgtcc
     7561 ggcgagtgcg ggtgcggcgg cagtcgacgg tcgagcggct ttcgccgttg ccggtggcgt
     7621 caggccgaga accggcgcat gcagggtcgc gagtgcgccg tgtgcttgtc gaacggccag
     7681 cgcggtcggt ggtgagcgat atcgggccgc gcctgcgcac tagcgctcgt cgccggcgcc
     7741 gtgaactcga ggagctcgac gcgctcagtg agtggatcga cctcgtcgcg ttgtcgtgtg
     7801 ccgcagggtc gacggtggca accgcggtgg aacgggcgac caccgccatg tcgtgggcgg
     7861 cgccgcttgg tcgcgagctg cgccgacaac ccagcatcga tcgcgcgctc gatgtgtggg
     7921 gggccgaggg gggcgatgat cgacgggcgc tgtcggccct gatcaacgtg agcctggtga
     7981 gtggatcagc gctgagcgag cggttgtcca atcggagcga ctcgctgcga tcccggcgtc
     8041 gtcacgaggc gctggccgct gctcggcgcc ttccggtgcg cctcttggtg ccgctcgtcg
     8101 tctgtgcctt gccgtcgttt gtggcgctga gcgtggtgcc gttgctcgtg gtgagccttc
     8161 agtcgctgtc ctgggagccg tgaacggcca ccgggtggcg caatcgcagc cctcgctgcc
     8221 gtcgtcctgc acattcggga gtcagttatg ttgaatcaac ctcattgttc ggcgtcgtgg
     8281 tcgcgtcgtc tccggtgcgt cgcgtggcgc gccgcgaccg accaacgggg ccaaagcacc
     8341 gtcgagtacg cgctgatact cgtcgcggtg acggtgatgg tgggtgtggt gatcgcctat
     8401 ctcgcgggcg acggtggcgg cacgatcacc gcgttgttct ccgccgcctt cgcgaggatg
     8461 cgaaatctcg tgggcggatg agcggtcggt tgtcgtgcct catcggaggc ggcgcatcgt
     8521 gacgcgtgca cggcgcggcc agcgtggtca ggcaacggtc gagatggcgc tggtccttcc
     8581 ggtgctgatt ctgggaatcc tgatcgtgtt gcagatcctg ctcgtcgctc gtgatgcggc
     8641 gtcgttgacc gccgccaccc gagcggctgc gagagcggcg atggtcgacg acgacccggt
     8701 cgaggcggcg cttcatgagc gccacctcga ccgatcacgc ctgcgggtcg aggtgagcgg
     8761 cactgccgcg gccggtgagt tgatcaccgt ttccgcgacg taccggtctc ccaccggttt
     8821 ggcggtcgtg ggtcgattcg tcggggatgt cacgttgacc gagcgattca cggtcaggaa
     8881 ggagtgattc gccggagtta tttatgtaaa tgctgtgttt ggtgtagcaa aagacagcga
     8941 cgtcgtgggt agcctggaag cgtgcacttt cgggtccgaa gcgtcgatcg cggacgatgg
     9001 acctcggtgt tggtcaccgg ggatgtcgat ctcgccgcac tgccgctgta ccgacgcgaa
     9061 cttcacgatg cggtcgccgg cgccaaaccc ctcgagatcg acctcagcga atgcgacttc
     9121 atggactcca cgggtctggg ggtcaccatc ggggcggtgc gacgggtgtg ggatcgcgac
     9181 ggcgaggtcc gaatcgtcac ctcgccaccg gtgcagcggc tgatgacgag atgccgcctg
     9241 gacgaaatcc tcccgttgac cgccccttcg gtgcagtctg cggacccggg ggaggttctg
     9301 gcgccgtgac cgatgagcag ttcgtcgagt tgatgatccc agcggagagc agctttgtcg
     9361 ccacggcgcg tcgcttcatc accgacgttg ccggcaaagc cgattggatg gatcagcaac
     9421 acctcgacga tctgcggctg atggcttcag aggcggtgac caatgcgctg cgtgcccaac
     9481 gtcgcgcgga ggtcggcgac aagatctgcg tccggtgcat gttgttttcc gatcgcttcg
     9541 agttcagcgt cgccgacagc gctggtgggt tcgaacgccc ggcccacctc gacatccaca
     9601 ccgacccgga actttctcgc gagggcgggt tcgggttgcc gttgatcgag gcgctagcgg
     9661 acgaggcgca tttcgtgaac accgacgggg gcacgacggt gcgcctcgtg gtttaccgac
     9721 ctgggtgttc gagcacgcgg gaatcgacat ccacaactcg gtgaacgatg gacatggcca
     9781 aacccgtcag gccaacgatc gccagacaga tccacaccga ttctgccgct ggcagggtga
     9841 gttgaaagta ctccttgccg agcggagtcg tgaacgccgc cacgacgccc aaccccatcg
     9901 tcgcgatcaa cccgagcttc cagccgcgaa tcggtcgtgc gtttctcacg agaaccaaca
     9961 gtgagcatcc cgcaagcgtg agcgtcgccg cggtctgttg ttcctccagg gtggtgccag
    10021 ttgttgaacg catgatgacg aacgcggcca tcgtggcggc cgcgcagagg attcccatcg
    10081 gtacggagtc ccaaccgacg cggcgcagga aattgccctg cacgcgagcc gtgttgggtg
    10141 cgagggccag aaagaacgag ggaattccga tgcagagtga actgatcagc gtcaggtgtc
    10201 gaggcaggaa cggaaagtcc atcgcgaaga tgccggtgac gaacgcgaag atcacggcgt
    10261 aggtggtctt ggtcaggtac aacgaggcga ctcgctgaag gttgttgatg acccgacggc
    10321 cctcggcgac gacggcggga aggcccgaga acgacgagtc caacaagacg acctgagcga
    10381 ctgcacgagt ggcctcggat cccgaggcca tcgcgatacc gcagtcggca tctttcagag
    10441 ccaagacgtc gttcaccccg tcgccggtca tcgccacgat gtggtcgcgg cgttgcaacg
    10501 cggtgaccat ggcgcgtttt tggtgaggtg tcactcgacc gaacaccgtt ccggcctcga
    10561 cgagatcggc gaactcgtcg tcggttccat cggaatccgg caaagttcgg gcgtcgacga
    10621 ggtggttgtc gacctcaagc cccgctcgtt cagcaaccgc acccaccgtg gtcgggttgt
    10681 cgccggagat gaccttgagg gtgacgccct gatcggcgaa ataccgcagc gtctccgcgg
    10741 cgtcgggtcg gatcttgtcg atgagcgaaa ccagcgcgac cgcgacgatg tcaccgggca
    10801 gggcccggtc cgaggacacg ggcgagtcgg tgtgtgccag caacagaatc cgcaaaccgt
    10861 taccggaggc cgcctcgatc tgctcgctga ccccggcgag ctttgcgtcg tcgaggatca
    10921 tctcggccgc tccgagcacc caccatccct ggccctcgaa ccggttggca ctccatttgc
    10981 gcgctgagga gaacggtacg ccgtcgagtc gttcccaccc cgaagccaac ggctcggaga
    11041 accgttcgat gagcgcttgt tgggtggcgt tcgggttcgg gtcggagtga gcgatcgccg
    11101 tgagcgcgag ggatccgagt tcatcgtcgt ggccgccgag cgcggtgacg gaatcgacga
    11161 ccatcgtccc ctcagtgatc gtgccggttt tgtcgagaca cacgacgtcg acccgcgcca
    11221 ggatctccac cgccggcagt tcctgcacca gcgtgcgccg gcgggcgaga cgaacgacgc
    11281 cgacgaagaa cgccaccgag gtcaacaaca acaggccttc gggcaccatg ccgacgagac
    11341 cggcgaccat ggcgacgagg gcggcccgga tgtcgtcgtt gctgcgcatc tgcgaccaca
    11401 ccaagccgat cgcggccggg atgagggccc acgacaccca ggtgacgatc tgttcgatgg
    11461 aggctcgcag ttcggagttg gcgagggtga agcggcgcgc ctcctcggcg agccgcgccg
    11521 cgtaggcgtc cttgccgacc ttggtgacgc gtgcgtagcc ggatccggtc acgacgaagg
    11581 atcccgacaa cagttcgtcg ccggtccctt tgacgacggc gtcgctttcg cccgtcagca
    11641 tcgattcatc gatttcgagg ccgtcgcagt ccacgagcac gcagtccgcc acgatctggc
    11701 cgccgggccg caattcgatg acgtcgtcga gcacgatgtc gttgaccggg tgatctcgca
    11761 cctctccgtc gcgtagaagg tgggcgtggg gcgcgttgag gagcgcgagc cggtcgaggg
    11821 ttcgcttggc gcggacctcc tggaaaatcc cgatgaagat gttcgcgacg atcacgccgc
    11881 tgaacatggc gtcgatcggt gatcccgcag cgatgacgat ggctgcgagc agtgcgatca
    11941 gcgcgttgaa ccgggtgaac acgttggcac ggatgatctc ggggatggtt cgactcgggg
    12001 ccgggggcac ctcgttgacg agtccggcgg cgatgcgttc gctgacctcg gttgaactga
    12061 gtccggtgat gcgctgcgac atagcgtcca tgggctagga gtacttaccg agttcggttc
    12121 gtgcgacctg tcggcggtgg acctcgtcgg ggccatccgc caggcgcagc gtgcgcaggc
    12181 cggcatacat ggccgcgagc gggaagtcgc tgctgacccc gccgccaccg tgggcctgga
    12241 tggcccgatc gacgacgcgc agcgcgacgt tgggtgccac gactttgata gaggagatct
    12301 cgatcgccgc cccgcggttg ccgacggtgt ccatcagcca cgccgccttc agcgtgagga
    12361 ggcgcgcctg ttcgatctcc atgcgcgaat cggcgatcca ttcgcgaatc acgccctgtt
    12421 cggccagcgg ccggccgaac gcgacccgct gggtgacgcg tcggcacatc agttccaggg
    12481 cccgttcggc gataccgatg gcacgcatgc aatggtgaat gcgcccgggg ccgaggcggg
    12541 cctgggccat catgaacccc gacccttcgg tgtgcagcag gttggtggcc ggaacacgga
    12601 cgttgtcgaa gtgcacctcg gcgtgcccct cacggtcggt gtagccgaag acctccaggt
    12661 tgcgaacgac cgtgacgccg cgggtgtcgc ggggcaccag gatcatcgac tgctgcagat
    12721 gcttcgctgc ggtgggatcg gtctttccca tcacgatgaa cagtgtgcag cggggagaca
    12781 tcgcacccga ggtccaccac ttgtggccat tgagcacgta ggagtcgccg tcccgggtga
    12841 tcgacaactc gatgttggtg gcatcggaac tggcgacgtc gggttcggtc atcgcgaagg
    12901 ccgagcggat ttcaccgttg aggaggggtt gcaaccactc ttctttttgg gcgtcggtgc
    12961 cgaacatggt gagcagttcc atgtttccgg tgtcgggtgc ggagcagttc agcgcctcgg
    13021 gcgcaaggtg catcgaccgc ccggtgatct ctgcgagcgg ggcgtagtcg aggttggaca
    13081 gaccgtcggt ccactgggtc ttgtggggaa ggaacaggtt ccacaacccg cgcgccttcg
    13141 cttcggcctt gaggtcctcg atgaccggtg ggtggtcgtg gggatcgagg ttcgccatct
    13201 ggtcgtggta ggtcgcctcg gccgggtaga ccttgtcgtc catgaactcg agcagccgtt
    13261 cctgcagcgc ggtggcggtt gggctgagct gaaaatccat gccatctcct cacgacgttg
    13321 gagccaccgt agcggtcgca ccggttcgga tttcgtaccc gtcccgggac taatcgttct
    13381 cccgatctcg cgagggtgtt tgacataccc gggaatcggg atattgagtg aagcccatgg
    13441 gccaacccct cgtcatcgtc gagtctcctg ccaaagcacg taccatttcg cgatttctcg
    13501 gcgacgagta cgtcgtggag tcgtcggttg ggcacatccg cgatctgccc tcgaaggcgg
    13561 cagaggttcc ggcggcgtac aagggaacgg tgctcgcaaa gctgggggtc gaccccgacg
    13621 acgggttcaa gccgctctac atcgtctcgc cgaacaagaa ggagcaggtc aagcgactca
    13681 aggaccttct gaaggacgcc gacgagctct atctcgcgac cgatgaggat cgcgaggggg
    13741 aagcgattgc ctggcacctg ctcgaggtgc tcaagccgaa agtgccggtg cggcgcatgg
    13801 tctttcacga gatcaccccc gacgccatcc aaagcgcgat cgaaaatccc cgcgacctga
    13861 accgcagcct cgtcgacgcc caggaggcgc ggcgcatctt cgaccggctc tacggctacg
    13921 aggtcagccc cgtgctgtgg aagaaggtca tgccgcggct ctccgctggg cgcgtgcagt
    13981 cggtcgcgac acgaatcgtg gtcgaacgcg aacgcgaacg tatgcgcttt cgcacggcga
    14041 gctactggga tctgtccgca accttccagc tcaccggtgc aggcaaggac acgaccccct
    14101 tcggagcgaa cctcatcgag gtcgattcgt tgcgcgtcgc gaccggacgc gatttcgacg
    14161 agaccggcag cctgacccga gacggtgcgg tcctgctcga cgaggaatcc gcaggcgcgc
    14221 tgcgggcggc gctcgagggt cgccctgcga cgatcgcgtc ggtcgaacgc aagccgtaca
    14281 cccgcaagcc gtacgcaccg ttcatgacct ccacactcca acaggaggcg ggccgcaagc
    14341 tgaggttcag ttcgagccag acgatgtcgc tggcccagcg cctgtatgag aacggcttca
    14401 tcacctacat gcgtaccgac tccacgacgt tgtcggaaac ggcgatcaac gcggcgcgaa
    14461 gtctcatcgc ctccaagtac ggcgaggcct acctgcccga ctccccccgg gtgtatgcga
    14521 agaagtcgaa gaacgcccag gaggcccacg aggcgatccg gcccgcgggc gacaccttcc
    14581 gcagccccga ggaggtgcgc tccgttctcg gcacccagga gttccgtctg tacgaactca
    14641 tctggcagcg cacgatcgcc tcgcagatga ccgacgcccg cggcgagagc atgcaggtgc
    14701 gcatcgacgt cgacgcgagc gacggtcgtc gtgcccgctt cgcggcgagc ggcaagacga
    14761 ttctgttccc ggggttcctt cgggcgtacg tggagggagc ggatgacccg gatgcggaac
    14821 tcgaagatca ggaacgcatc ctgcccgaca tggccgaggg cgacggtgcg cgcagcaccg
    14881 aactcgacgc caagggccac gagacccagc cacccgcccg ctataccgaa gcctcgttgg
    14941 tgaagcgtct cgaggagctc ggcatcggcc ggccctcgac gtacgcgtcg acgatctcga
    15001 cgatccagga tcgcggctat gtgcgcaaga agggctccgc gctcgtgccg tcgttcacgg
    15061 cgttcgcggt ggtgaccctg ctggagcagc acttcccgga tctggtcgac tacgcgctga
    15121 ccgcacacat ggaggacgat ctcgaccgga tcgccaccgg agacgcctcc tcggcaccgt
    15181 ggctgagttc gttctacttc ggcgatgggg tcgacagcga ccgtcccggg ctcaagcaca
    15241 tggtcaccga catcgaacac atcgacgctc gggcggtgaa ctcgattccg atcggagcgg
    15301 atgccaacgg cgagctcatc gtcgcccgtg tggggcgtta tggggcctac atccagcgcg
    15361 gcgacgatac ggcgaacatt cccgacgatc acgcccccga cgaactcacc cccgacaagg
    15421 ccgtcgagct gttgaacacg cccaaggagc gcaagctcgg cgatgatccg gcgaccggca
    15481 agccgatcta cgtgaagaac ggtcgcttcg gtccgtacgt gcagcttggc gaccacgacg
    15541 acgagaccgg cgagaagccg aagatggcgt cgttgttcca gacgatgacg ctcgaacggg
    15601 tgagcctcga tgacgccttg gagctgctga gtctgccccg agtgctgggt gtcgacccgg
    15661 cggacggggt cgagatcacc gctcagaacg gcaagttcgg gccctacatc aaaaaggaca
    15721 ccgatagccg tacgatcgaa aacgaggaga tgctgctcac catcaccctc gacgaggcgc
    15781 tgcggatcct ggccgagccg aagcgacgcc gcggccaggc ggtgaagggg ccgttgaagg
    15841 acctgggcaa agacccggat tctgagcgcc cgatcctgtg caaggacggt cggttcggcc
    15901 cctatgtcac cgacgggatc acgaatgcct cgcttcgcaa gggtgacagc gtcgaggagt
    15961 tgacgatcga acgcgccatg gagttgctgg ccgatcgtcg cgaaaaggac atcgccgcgg
    16021 gcaaggatcc ggccaagaag gcctccgcca ggaaggctcc ggcgaaaaag gccggcgcga
    16081 aaaagaccgc ggccaagaag gctccagcca agaagaccgc ggcgaagaag gctccggcga
    16141 agaaggccgc ggccaagaag tcgccgaccg cggcggtcac tccgaaagcg gtggcggacg
    16201 cgttcatcgc tgcggacggc gacgagccgt tctgatgtcc tcgaccgccg ccggcgggac
    16261 cggccgtgtg gaccgcacag cgccgttcat cgtcttcgag ggtggagagg gaggcggcaa
    16321 gaccacccag gcggccctgc tgcgggagcg actggccgat cgtgtcggaa tcgaagccat
    16381 cgtgacccgc caaccgggcg gcaccgcgat cggggccagg ctgcgttcga tcctgttgga
    16441 cgtggacaat gccgaattga gtgaccgctg cgaggcgttg ctcatggcgg cggatcgtgc
    16501 gcaacacgcg accgaactca tcggtccgac cctggcgtcg ggggtggggg tgatctgcga
    16561 ccgctatctc tacagttcgg tggcctacca gggctacggg cgaggcctcg acgcgcagaa
    16621 ggtcgctgaa atcagcgagt gggcgaccgc cgcgctgttg ccggacctgg tcgtgttgat
    16681 cgacgtcgat ctcgacacgg cgtcgggccg cctgcagcga gaactcgacc gcttcgagca
    16741 ggcgggtgcc gactttcatg aacgggttcg ccgggggttc ctggcccagg cgaacgagga
    16801 cccgtcgcgc tgggtggtca tcgacgggag ccggtcgatc gacgaggtgg ccgaacagat
    16861 ctgggatgcg gtggcccgac tgccgatggt gacgcaatga cgaccggcat ggaggcgacc
    16921 tccgacgacc tcgatgacct gtggagcgaa gtcgtcggcc aggaccaact cgtcgcacaa
    16981 ctgcgccgcg ccgcggccga tccaactcat gcgtatctgt tcgtgggcgc tgccggggtc
    17041 gggacccgcg ccgcggcccg tgcattcgcc gccgaactgt tgagcggcgg cgccgactcc
    17101 gtcgacatcg ccaggcattg cgcgctcgcg aaggcagagc accacccggc gattcgcttc
    17161 gtcgagcgaa cgggggcgtc gatctcggcg gatcaggcgc gcgagatcgt gcgtcaggcg
    17221 cagatgcgtc cgcccgaggg cgacctgcag gtgatcgtcc tcgtcgactt tcacctggtg
    17281 agcacggctg ccccgatcct gttgaaatcg atcgaggaac ctccgtcggg gacggtgttc
    17341 atcgtgttgg ccgacgaggt gccgccggat ctggtgacga tcgcgtcgcg ctgtgtcacc
    17401 ttcgagtttc cgatcctgtc gaccgcgctg atcgccgagt gtctgcgacg cgacggggtg
    17461 agcggcgacg tggccgacgc cgcggccgcg ggcgccgcgg gaaacatcga ccgcgcgcgg
    17521 ctgttggcca ccgatccgct ggtggttgcc cggcgacagt tctggatcga tcttccgggc
    17581 cgactcgacg gaaccggagc gaccgccgcc aggctcgcgt cggaggtcat cggccgcgtc
    17641 gatgaggtgc tcgaaccgct cgcggagctg cacagccgag aactgaccaa gctcgccgaa
    17701 caggccgaac ggttggggtt acgcaagtcg caacaggctg aggtcgaggc gcgtcacaaa
    17761 cgcgagcagc gtcgggtgcg cagcgacgag cttcgagccg gtcttgcggc gctgatggag
    17821 gggtatcgcc gcgacgccgc gacggccccc gacgcattcg tcgccgctgc cgaactcgtt
    17881 ggcgagttca ccgaacgact ggtgta
//
LOCUS       DAOOMA010000002        15308 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_4131_length_15308_cov_4.469154, whole genome shotgun sequence.
ACCESSION   DAOOMA010000002 DAOOMA010000000
VERSION     DAOOMA010000002.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 15308)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15308)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15308
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4131_length_15308_cov_4.469154"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..288)
                     /locus_tag="PLP95_00095"
     CDS             complement(<1..288)
                     /locus_tag="PLP95_00095"
                     /inference="COORDINATES: protein motif:HMM:NF019575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HPB44235.1"
                     /translation="MKALLVARRRAMNSQRGLTLMEVLLTSAISTIIAVPVLAWMITA
                     FRTDAVVRATSTRTQLVTQITQYFPRDVASSAGVIYTSEGGAAPGVVNYDCE"
     gene            complement(285..737)
                     /locus_tag="PLP95_00100"
     CDS             complement(285..737)
                     /locus_tag="PLP95_00100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HPB44236.1"
                     /translation="MAVAASPRRRVSQCGGTLVEALLAIAILSILLLGVMAALTTTAT
                     VSRSTGQIAATRSALSTVIERAGAAAYPGCDAAGAVDTRLHDSTSRSLVEAPDGYGFE
                     VVAVASAHPQAASCQASTSAVLVTIRVSHLDSGEALDGQVVIRDRGTP"
     gene            complement(934..1785)
                     /locus_tag="PLP95_00105"
     CDS             complement(934..1785)
                     /locus_tag="PLP95_00105"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: protein motif:HMM:NF014296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0006555 - methionine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HPB44237.1"
                     /translation="MTRISDLLAEGPTLSFEFFPPATAAGVDALDAAVAELAVLDPSF
                     VSVTYGAGGSNRDRTRQIVARITESQRYPAMPHLTCIGHTREELVDLVDGYLAAGIEN
                     ILALAGDPPADGSPAIGDFEHAADLVELIRTRSDAAVGVAAFPEGHPRSASLSQDRRH
                     LAAKLQRADFGITQFFFRSEHYLAMRDDLETLGCEVPVLPGLMPMLNPTAVKRFADMN
                     GTWFDTALADKVEAADESDRLMIAADAAAELANELIAAGTPGIHVYCLNRAATAIALV
                     ERLNAFR"
     gene            complement(1782..2663)
                     /locus_tag="PLP95_00110"
     CDS             complement(1782..2663)
                     /locus_tag="PLP95_00110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetrahydrofolate dehydrogenase/cyclohydrolase
                     catalytic domain-containing protein"
                     /protein_id="HPB44238.1"
                     /translation="MTAQILDGDAAAAAVKDDLRGRIAALAERGVVPGLGTILVGDDG
                     PSHKYVAMKHADAAELAMATTDIRLPQDATQAEVEAAVAQLNADPAIDAYIMQYPFPK
                     HLDFAAAMEKVLPRKDADGIHPFNLGLLVMGEEAPLACTPAGIVRLLEHHDIEIAGRH
                     VVIIGRGLTIGRPLANLLSLKSVWGNAAVTVVHTGVQDIGQFTRSADIVVAAAGSPAM
                     LTKEMIRPGAVVVAAGVSFVDGKVVSDAADDVAEVASWLSPRVGGVGPMTRAMLLANT
                     VAAAERSTDAAALGIPL"
     gene            complement(2697..4466)
                     /locus_tag="PLP95_00115"
     CDS             complement(2697..4466)
                     /locus_tag="PLP95_00115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPB44239.1"
                     /translation="MSSHPFMRLWHHARRHRSRLVLAAFFSTFNKACDVAPELLIGVA
                     IDVVVRGDTSMLSRVVGVTDRFDQLLVLAALNVAVWILESLSDYIAHLLWRGLAQSIQ
                     HDFRIGAYRHVQQLEVAWFEDTTSGGLLAVLNDDVNQLERFLDHGPDNILQTFWNVVF
                     VGIVFFTTSPTLWLFAFLPIPIIVVGSIRFQRRLEPLYADVRERVADLSSLLSGNLGG
                     ITTIKAFTAEEREADRLEAVSQAYVDSNRRAIRYSSAFVPLIRMVILAGFTCTLVVGG
                     RMVIDGTLEVGSYSVLVYMTQRLLWPLTSLGETLDLYQRSMASIRRITDLLEVQPKLV
                     GGPDALDEPVRGEVELRDVHFGYGNGPDILDGISMRIPAGQTHAVVGATGAGKSTLAR
                     LVLRFHDPRSGEVLVDGRDVSQLGFASLRQAIGYVSQDVFLFQGTVRENLAFGRPDAS
                     EEEIVTAAQLAEAHEFIKALSDGYDTIVGERGQKLSGGQRQRLSIARAILRDPAILIL
                     DEATSAVDNETEAAIQRSLARVAEHRTALVIAHRLSTVRDADRIWVLEAGGVAESGTH
                     DELVAAGGMYAALWRVQTGEVSS"
     gene            complement(4520..5494)
                     /locus_tag="PLP95_00120"
     CDS             complement(4520..5494)
                     /locus_tag="PLP95_00120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB44240.1"
                     /translation="MFEQSDVIRTATRLNPAWSDPDAIVSLIRSAGPLSPLANYAAND
                     AEMATLSTQENSFTPPWFRQDFVRGGEVLVDGAEVIFNNPNFLAAARELAGPDAVLRP
                     TAVYVNVMGPTPFAFPAHLDIAAFRGITRAHYPIWLLKVMMSSGLFEEWRTTIFTAVS
                     WFYDGEGGDFHFWPEGPSGTVGVESPPFRNVGVIADNETTFHGVAPLGAPGATMPEGL
                     HSGCHLVRGDGGWDIVDGDRLVSRIDDADARITVSWKAECFADAEAAQRVDDGEDALT
                     LDMVVDRFLDDLAARGIEAERPADPLGDEAFVAVLAAAYPEVAPELVA"
     gene            complement(5494..8085)
                     /locus_tag="PLP95_00125"
     CDS             complement(5494..8085)
                     /locus_tag="PLP95_00125"
                     /inference="COORDINATES: protein motif:HMM:NF040071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6351 family protein"
                     /protein_id="HPB44241.1"
                     /translation="MTDLVVSVHRANSAGRPRGTKAARRFVEALLAATALVAVTATTV
                     VPAGASALDDSAEVEVQEPFLCTTEYNDLGQPNVDNQDRRGTPVYPESEPDTPDRSQE
                     PLGWSKDCQADTRIEYRYRNTDGEVVPLNVGDGVDATLPADVAYLAVSDLVGADDMDL
                     GGDTRIPYLYRYERGTLPDTRFIYSVAMLVPFSEVVSGAPDDSHWNGRLVYSFGGGVG
                     IGHSQGSLSGGDSQLDEALRLGHAVVYSSGTRTSVHYNLLRGAEVAKELKARFIDQHG
                     EPLYTVGIGGSGGGIQQYVYAQNVPDLLDALIPQYAYPDMTTQTINVGDCELLEYYMD
                     VTAADDPMWKDWDNRKILQGQNTIEGFSSDWSEATGDSGSSECIEGWRGATPLALNPN
                     FGLAADMDDALMPFAGELLSLAGAGDPAVPEDFPDIGAMLRTSTDRSEWVDWTHWDDA
                     VDAYGTDPATGEALVPWDNVGVQYGLRAVANGQITPDQFLDLNARIGSWKPASEQVPE
                     SCGMVAQMTGETLGAFAEAVGMCEGDELDQYSSRQMNLELVNGVAPRREADVAAITNA
                     FESGLEFDGKLGRDIPILDVRHYLEDELDMHNVHQSFVIRERIRRAMGAADNHVVWFA
                     DARPGEDTEATSALLDRVYRVMDEWVLALHNDADVTAASAKPVEAVDACFATDGTPIA
                     SGDHVWDGAVELVLSGEGAWTSAAPREVDGVEVGDCASQFPIHSTSRVVAGGPITNDV
                     YKCHLKPVATALADGDYGSWTPDESEMQRLEAIFATGVCDWSKRSVGYPADLASGGGA
                     ADSGPAEGHSTDDDSDSLPWIFLGAVGGLIVVGLALRLVARRGDGSEDPDVDMMVDMT
                     AATETSE"
     gene            8279..8521
                     /locus_tag="PLP95_00130"
     CDS             8279..8521
                     /locus_tag="PLP95_00130"
                     /inference="COORDINATES: protein motif:HMM:NF017691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excalibur calcium-binding domain-containing
                     protein"
                     /protein_id="HPB44242.1"
                     /translation="MKRTLCLLAAMALALTTTGCEKFPNCDALRVQFPHGVGLPGAID
                     STSGSNPITDFQVEETYYKFNSHLDRDNDGIACEQA"
     gene            complement(8544..8705)
                     /locus_tag="PLP95_00135"
     CDS             complement(8544..8705)
                     /locus_tag="PLP95_00135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB44243.1"
                     /translation="MIGPELASTPERHRSIGEVRGLGVFWAIELVRYRETREVFVPYN
                     ASGADAFVA"
     gene            complement(8766..11315)
                     /locus_tag="PLP95_00140"
     CDS             complement(8766..11315)
                     /locus_tag="PLP95_00140"
                     /inference="COORDINATES: protein motif:HMM:NF014717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HPB44244.1"
                     /translation="MLIIIRRNLTAHPRRYVATVGAVAVGIAFLFAVLVEVATFSRSF
                     DDTMRSSVAGIDASIRSANSISTNEETYRGHVPADLAEIARAVDGVRIAEPAYSRPAM
                     IVGSDGDTIGGSGPPQLGFEWRDDPDLNPFRIAEGRAPQGLDEVVIDRASARQGDLSV
                     GDTIEVLVPKRLRLRLVGIAAYGTAESAGPMTATLLSPDAAAEYLADPGTAASISVVA
                     LDGISQSELVERLRTALDESAAAGSLEVVTGQELQDEAAKVSETFSTIVSAVLLTFAS
                     IALVVAAFGIYNTFAILVAQRTRESALLRALGASRRQLSGWTLGESFAVGVLGSAVGL
                     GFGLLLARMLSWLIGRVGFFSLGDHLVVSASSVIIGAGVGIVSTVIAAIAPARRGSRI
                     APVEAMRSAAIDGGSVGRRRTILGAALMAAGVGLAVQAALSANSGASTRAGVGAAITL
                     LSVVVIGPALAPALVRVIGAPIARTRGTAGLLAQRNAVRHPRRTASTVTALVIGVAVV
                     TLFTVVAASLNRAVTRSVDEQMAGDVIVQPTSFSGSGLDDALVDEVASSDGVDSSLPL
                     SFMPVTVGGETAFVTATDMAAGAAMFDLGVIDGSVAGATGDVIAVSATWASDHDVAVG
                     DALPTRFLDGVELQLRVVALYDNTAFAGDYVMPADTAGSHSPGVSTSLVVLRASEGVS
                     AIELDRRIDMLISDAPGVESLDREDYAETIGRRIDTMLALVYGLLALSIGIALLGIAT
                     TISLATMERVREIGLMRALGQTRRQVRTTVRLEAIMIATFGTIIGLAVGMFAAWAVLT
                     SSSDELLRTVSFPVSRLVMILFLGALAGIAAARRPARRVARLDVLEAIAAP"
     gene            complement(11370..12140)
                     /locus_tag="PLP95_00145"
     CDS             complement(11370..12140)
                     /locus_tag="PLP95_00145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPB44245.1"
                     /translation="MNTSALRAPISEVAATDTALRADILSKRYGDGPGALDALSNVSL
                     SVASGSFVAIMGPSGSGKSTLMHCLAGLDTPTSGMIEIAGIDISGLDERRLTELRRDR
                     IGFIFQAFNLVATLTAAENIRLPSMLARTRLDESWYHEVVRRVGLEGRLGHRPSELSG
                     GQQQRVAVARSLVGRPEVIFADEPTGNLDSGSGDEVLRLLRSAVDEVGQTIVMVTHDP
                     GAAAYADRVVFLADGNVVDELEAPSASAVLDRMKRFGD"
     gene            complement(12222..13079)
                     /locus_tag="PLP95_00150"
     CDS             complement(12222..13079)
                     /locus_tag="PLP95_00150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB44246.1"
                     /translation="MTSSHKLARPALLLVGAVLSFGSVGTASFAIATGLVASTTDSTA
                     RYDATAIDRIVVHNAGRVRVTGVPDAIESAEASGSAESADGPEFPAASAPDGNSVAPD
                     EITVRRTLYQSFSEPTIDDHVEGTTLYLESRCSGLAGRICFGDVEIVAPERVAVDIGG
                     NDVQVRNTSGSVTITVRAGGASLSNVSGPLELDIFGGGVQATGVRSEQVSATLVAGAL
                     DMAFDASPLSVDAAVTGGSINLAVPSDATSYRIDSSGSTTVLVPVDPASRHRLNLSAR
                     GGNIRVANR"
     gene            complement(13076..13726)
                     /locus_tag="PLP95_00155"
     CDS             complement(13076..13726)
                     /locus_tag="PLP95_00155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015439770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HPB44247.1"
                     /translation="MRIVIVEDSVLLRDGIARLLQDAGDEVVATAGDAESGLAAVEAY
                     RPDIVVMDVRMPPTHTDEGLRAAIAIRARHPEIAVLVLSQWVEERYATELLAGSTVGV
                     GYLLKDRVADVAEFVDAVRRVGTGGTALDPEVVAQLLSTSRHRNPLDSLTQREAEVLA
                     LMAEGCSNRAVAAAMVVSEGAVEKHVSNIFAKLSLAPADTDHRRVLAVLRYLEVTS"
     gene            complement(13723..>15308)
                     /locus_tag="PLP95_00160"
     CDS             complement(13723..>15308)
                     /locus_tag="PLP95_00160"
                     /inference="COORDINATES: protein motif:HMM:NF025168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HPB44248.1"
                     /translation="APAPVTSNHPDASQPGAIGRASLVVRPLLAPFAPSTWVAFAYLA
                     VDAMWSLVTTSVMVALVTATVATVFFPPAAIALAAFAFAFSNGLARVTRWIAATALNE
                     SLPSPHPPRAAVHPPATMTPATMTPATMTPGTGGDAAPSPPSTLRQATDRGVAYLKQT
                     GTWKELIFHLLHAPVSWLFFTIAVAVWAVGIALVLVPLYTPRSPGGAGNLLSVSLDGA
                     VEYTVAAGVGLALVLIAPWLTRGLSTVLLSLTRQIISTDLIELELRERVDTLETTRAG
                     ALHAAEVERRRIERDLHDGAQQRLVALAMTLGLARQKVDGDREELRSLLDEAHGEAKA
                     ALTELRDLARGLHPPVLTDRGLAAAVGGLAARSPVPVAVDVQLDVRPPRAIESTAYFV
                     ISESLTNVARHSGATRSSVRVFEVRPPGSGKDHLVVEVADDGHGGASDPTAGAPSIGE
                     PAARGLEPTQRSGLLGLRERVEAVDGLLTVSSPPGGPTVLRAVLPVPPPSPPTPPPPI
                     EPSSSPLESARRITEGPSS"
BASE COUNT         2766 a         5130 c         5008 g         2404 t
ORIGIN      
        1 ttcgcagtcg tagttgacca cgcccggggc ggcgccgccc tcgctggtgt agatcacccc
       61 ggccgatgag gccacatccc gagggaagta ctgggtgatc tgggtgacga gttgtgtgcg
      121 ggtggaggtc gctcgaacga cggcatcggt acgaaatgcc gtgatcatcc acgccagcac
      181 ggggaccgcg atgatcgtgg agatcgcgga ggtcaacaac acctccatca gcgtcagccc
      241 ccgctgactg ttcatcgccc gacgtcgagc gaccagcagc gctttcacgg cgtcccccga
      301 tcccgaatga ccacctgacc gtcgagcgcc tcacccgagt cgagatgact cacccgaatg
      361 gtcaccagca ccgcgctggt ggaggcctgg caggaggcgg cctgcgggtg cgccgaagca
      421 acagcgacca cttcgaatcc gtatccgtcg ggcgcctcga ccaacgagcg gctcgtcgag
      481 tcatgcaaac gcgtatcgac cgctcccgca gcgtcacacc ccgggtacgc agcggctcct
      541 gcccgctcga tcaccgtgga caacgcgctc cgcgtcgccg caatctgccc ggtcgaccgt
      601 gacaccgtgg ccgtggtggt cagtgccgcc atgaccccga gcagcaggat gctcaagatg
      661 gcgatcgcca acagcgcttc gaccaaggtg ccaccgcatt gcgaaacgcg acggcgcggc
      721 gacgcggcca cggccatgac aacagcgggc gcctgttccg gcgccccgcc cgatgtccga
      781 atctgtcgtt tcgacctcgc cccgctcacc gtgggccgaa tcgtatcgtc gtcattcact
      841 gccgttcttc accgtctaag ggctcgccgg gttcgccgcc cgacgctccc gcaaagactg
      901 tgcaggccgc gccgcctcgg gtcaaggcaa gaatcaacga aatgcgttga gacgttccac
      961 caatgcgatc gcggtggctg cccgattcag gcagtagacg tggatccctg gggttcccgc
     1021 cgcgatcagt tcgttggcga gctcggcggc ggcatcggct gcgatcatca gccggtcgga
     1081 ctcatccgcg gcctcgacct tgtccgccaa cgccgtgtcg aaccacgtcc cgttcatgtc
     1141 ggcgaaccgt ttgacggcgg tggggttcaa catgggcatc agacctggca ggaccggcac
     1201 ctcacagccc agcgtttcga gatcgtcgcg catcgccaag tagtgctcgg agcgaaagaa
     1261 gaactgggtg atgccgaaat cggcccgctg caacttggcg gcgaggtggc gacggtcctg
     1321 ggacagcgac gccgaacgcg gatgcccctc gggaaacgcc gcgaccccca ccgctgcgtc
     1381 ggaacgtgtt cgaatcaact cgacgaggtc ggcggcgtgc tcgaaatcgc cgatcgccgg
     1441 cgatccgtcg gccgggggat cgccggcgag cgccaggatg ttctcgattc ccgcggcgag
     1501 gtagccgtcg acgagatcca ccaattcctc gcgggtgtgg ccgatgcagg tcaggtgagg
     1561 catcgccggg taacgctgcg attcggtgat ccgagcgacg atctgacggg tgcgatcgcg
     1621 gttggaccct ccggccccat aggtcaccga gacgaacgac ggatcgagca ccgccagttc
     1681 ggccacggcg gcatcgagcg cgtccacccc agccgcggtg gccggtggaa agaactcgaa
     1741 cgacagcgtc ggcccctcgg ccaacagatc ggaaatcctg gtcacagcgg gattccgagc
     1801 gctgcggcgt cggtcgatcg ttcggccgca gcgacggtgt tggccaggag catggccctc
     1861 gtcatcgggc cgaccccacc cacgcggggc gaaagccacg acgccacctc ggcgacgtcg
     1921 tcggcggcat ccgagaccac cttgccgtcg acgaagctca ccccggccgc cacgacgacc
     1981 gcgcccgggc ggatcatctc cttggtcagc atcgccggac tgcccgccgc agcgacgacg
     2041 atgtcggcac tgcgggtgaa ctgcccgatg tcttggactc cggtatgcac gaccgtgacc
     2101 gcagcgttgc cccaaaccga cttcaaactg agcaggttgg cgagggggcg gccgatggtg
     2161 aggccccgcc cgatgatgac cacatggcgg cccgcaatct cgatgtcgtg gtgttcaagc
     2221 agcctcacga tccccgcggg ggtgcacgcc aggggggcct cctcgcccat caccagcaga
     2281 cccaggttga acggatgaat cccgtccgcg tccttgcgcg gcaacacctt ttccatcgct
     2341 gcagcgaagt cgagatgctt cgggaacgga tactgcatga tgtacgcgtc gatcgccggg
     2401 tcggcgttca gttgcgccac cgccgcttcg acctcggcct gggtcgcgtc ctgaggcagc
     2461 cgaatgtcgg tcgtcgccat tgcgagttct gccgcatcgg cgtgtttcat cgccacgtac
     2521 ttgtgcgacg ggccgtcgtc gccgaccagg atcgtgccaa gtcccggcac gaccccgcgt
     2581 tccgccagcg cagcaatccg cccgcggagg tcgtctttca ccgcagctgc agccgcatca
     2641 ccatcaagta tctgtgccgt catcgtgcgc cagtctacgc gaccacctgc gcaggctcag
     2701 gagctcacct cgccggtctg cacccgccac agcgccgcgt acatgccacc cgcggcaacc
     2761 agttcgtcgt gggttcccga ctcggccacc ccgcccgcct caaggaccca gattcgatcg
     2821 gcgtcgcgca ccgtcgacaa gcgatgagcg atcaccagcg ccgtgcggtg ctccgccacc
     2881 cgggcgagcg accgttgaat cgccgcctcg gtctcgttgt cgacggcgga ggtcgcctcg
     2941 tcgaggatca ggatcgccgg gtcccggaga atggcgcggg cgatggacag ccgttggcgc
     3001 tggccacccg agagcttctg gccccgttcg ccgacgatcg tgtcgtagcc atccgacaac
     3061 gccttgatga actcgtgtgc ctcggcgagc tgtgccgccg tgacgatctc ttcctcggac
     3121 gcgtcgggac gaccgaaggc caggttctcc cgaaccgttc cctggaagag gaagacgtcc
     3181 tgactgacgt agccgatggc ctggcgcaac gacgcgaagc cgagctgtga cacgtcgcgg
     3241 ccgtcgacca gcacctcgcc cgatcgaggg tcgtgaaacc gcaacacgag acgggcgagg
     3301 gtcgactttc ccgcccctgt cgcccccacg acggcatggg tctgccccgc cggaattcgc
     3361 atcgagatgc cgtcgagaat gtcgggtccg ttcccgtagc cgaaatgcac gtcgcgcaac
     3421 tccacctcac cgcgaaccgg ctcgtcgagt gcgtcgggtc cgccgacgag tttcggctgc
     3481 acctccaaca ggtcggtgat acgacggatc gacgccatcg aacgctggta gagatccaac
     3541 gtctcaccga gcgaggtgag cggccacagg agccgttggg tcatatagac gagcaccgaa
     3601 tacgaaccca cctcgagggt gccgtcgatc accattcgtc caccgacgac cagcgtgcag
     3661 gtgaaccctg cgaggatcac cattcggatc agcggaacga aggccgacga gtaccggatc
     3721 gcccgccggt tcgagtcgac gtaggcctgt gacacggcct cgagacggtc ggcctctcgt
     3781 tcttcggcgg tgaatgcctt gatcgtggtg atgccgccga ggttgcccga cagcagcgag
     3841 gacaggtccg cgacccgttc ccggacgtcc gcgtacaacg gctcgagtcg gcgttgaaac
     3901 cggatcgacc ccaccacgat gatcgggatc ggcaggaagg cgaacaacca cagcgtcggc
     3961 gaggtcgtga agaacacgat gccgacgaac acgacgttcc agaaggtctg caggatgttg
     4021 tcggggccgt gatcgaggaa ccgttcgagc tggttcacgt cgtcgttgag caccgccagg
     4081 agtccgccgc tcgtcgtgtc ctcgaaccag gccacctcga gttgctggac gtggcggtag
     4141 gcgccgatgc ggaaatcgtg ttggatcgac tgcgccaggc cgcgccacag caggtgcgcg
     4201 atgtagtcgc tgagggattc cagaatccac accgcgacgt tcaatgcggc caacaccagg
     4261 agctgatcga aacgatcggt gactccgacg accctcgaca gcatcgacgt gtcgcctcgc
     4321 acgaccacat cgatggcgac cccgatcagc agttctggag cgacatcgca cgccttgttg
     4381 aaggtcgaaa agaacgctgc gagcacgagc cgcgagcggt gacggcgggc gtggtgccac
     4441 agccgcatga acgggtggga ggacatgggc gaagtgtaag cacgaccacc gtgacgcttc
     4501 gcaggagttc gacctgtgtt caggcgacga gttcgggggc gacctcgggg taggcggcgg
     4561 cgagtaccgc aacgaacgcc tcgtcgccga gtgggtcggc ggggcgctcc gcctcgatgc
     4621 cgcgcgctgc gaggtcgtcg aggaaccggt cgacgaccat gtccaacgtg agcgcgtcct
     4681 caccatcgtc gacacgctgc gccgcctccg catccgcgaa gcactccgcc ttccacgaga
     4741 ccgtgattcg tgcgtcggca tcatcgatgc gcgagaccaa ccgatcgccg tcgacgatgt
     4801 cccaaccgcc gtcgccccga acgaggtggc atcccgaatg caggccctcg ggcatcgtcg
     4861 cgccgggtgc tccaagcggc gccaccccgt gaaaggtggt ttcgttgtcg gcgatgacgc
     4921 cgacgttgcg gaacggtggt gactcgacac cgacggttcc gctcggccct tccggccaga
     4981 aatggaagtc gcctccctcg ccgtcgtaga accacgacac cgccgtgaag atggtggtgc
     5041 gccactcctc gaacagccct gatgacatca tcaccttcaa cagccagatc ggataatgag
     5101 cgcgggtgat gccgcgaaac gccgctatgt cgagatgcgc aggaaacgcg aacggagtcg
     5161 gacccatcac gttcacgtag accgcggtcg gtcgcagcac cgcgtcggga ccggcgagtt
     5221 cacgggccgc ggcgaggaag ttcggattat tgaagatcac ctcggccccg tcgacgagca
     5281 cctcgccgcc acgaacgaag tcctgacgga accacggtgg ggtgaaggaa ttctcctgag
     5341 tcgacagcgt ggccatttcg gcatcgttgg ccgcgtagtt cgctagcggc gacagtggcc
     5401 ccgcgctgcg gatgagcgac acgatcgcgt ccggatcgga ccaagcaggg ttcaggcggg
     5461 tcgctgtgcg gatcacatcc gattgttcaa acatcactca ctcgtctcgg tcgccgccgt
     5521 catatcaacc atcatgtcaa cgtccgggtc ctccgacccg tcaccgcgtc tggccacgag
     5581 ccgcagggcg agtccgacca cgatgagccc accgacggcg ccgaggaaga tccacggcaa
     5641 cgaatcggag tcgtcgtcgg tcgagtggcc ctcggcgggg cccgagtcgg cggctccccc
     5701 gcccgaggcc aggtcggcgg ggtagcccac cgagcgcttc gaccaatcgc acacgcccgt
     5761 cgcgaagatc gcctcgaggc gctgcatctc ggactcgtca ggggtccagg agccgtagtc
     5821 cccgtcggcc aacgccgtcg cgaccggctt gagatgacac ttgtagacat cgttggtgat
     5881 cggaccgccg gcgacgacgc gagacgtcga gtgaatcggg aactgagacg cacaatcgcc
     5941 gacctccact ccatcgactt cgcgcggcgc ggcggaggtc catgctccct cgccactcag
     6001 cacaagttcg accgcaccgt cccacacgtg gtcgccggac gcgatcgggg tgccgtcggt
     6061 ggcgaagcac gcgtccaccg cctcgactgg tttcgccgac gccgccgtga cgtccgcgtc
     6121 gttgtgaagc gccaagaccc actcgtccat cacgcggtac acgcgatcga gcagcgccga
     6181 ggttgcctcg gtgtcctcgc cgggacgggc gtcggcgaac cacacgacgt ggttgtcggc
     6241 cgcacccatc gcccgccgta tccgctcgcg gatgacgaaa gactgatgga cgttgtgcat
     6301 atcgagttcg tcctcgagat agtggcgaac gtcgagaatc gggatgtcac ggccgagctt
     6361 tccgtcgaac tcaagacccg actcgaacgc gttggtgatc gcggcgacgt ccgcctcgcg
     6421 ccggggcgcc accccgttga cgagttcgag gttcatctgt cgcgacgagt actggtcgag
     6481 ttcatcgccc tcacacatcc ctaccgcctc ggcgaacgca cccagggtct ccccggtcat
     6541 ctgagcgacc atgccgcacg actcggggac ctgctcagaa gccggcttcc atgacccgat
     6601 ccgagcgttg agatcgagga actgatcagg agtgatctgc ccgttcgcca ccgcgcgcag
     6661 cccgtactgc accccgacgt tgtcccacgg caccaacgcc tcccccgtgg ccggatccgt
     6721 tccgtacgca tcgaccgcat cgtcccaatg ggtccagtcg acccactcgg aacggtcggt
     6781 ggaggtgcgc aacatcgctc cgatatccgg gaagtcctcc gggaccgccg ggtcgcctgc
     6841 ccccgcgagg ctcaagagct cgccggcgaa cggcatcagt gcgtcatcca tgtcggcagc
     6901 cagtccaaag ttcgggttca acgccagcgg tgtggcccct cgccagcctt cgatgcactc
     6961 gctcgagccc gagtcaccgg tcgcttctga ccagtccgag ctgaacccct cgatggtgtt
     7021 ttgcccctgg aggatcttgc ggttgtccca gtccttccac atcggatcgt cggccgcggt
     7081 gacgtccatg tagtactcca acaactcgca gtcaccgaca ttgatcgtct gggtggtcat
     7141 gtcgggatag gcgtactgcg ggatgagcgc gtcgagcaga tccggaacgt tctgtgcgta
     7201 gacgtactgc tgaatcccgc caccggaccc tccgataccg acggtgtaga gcggctcgcc
     7261 gtgttgatcg atgaaacggg ccttcagttc ctttgcgacc tccgcacctc gcaacaggtt
     7321 gtaatgcacc gaggtgcggg tgccgctcga atacaccacc gcgtggccga ggcgcagcgc
     7381 ctcatcgagt tgcgagtcgc cgcccgacag ggagccctgg ctatggccga tgccgacccc
     7441 tccgccgaag ctgtagacga gtcggccgtt ccaatgactg tcgtcagggg ctccggagac
     7501 gacctccgaa aatggcacga gcatcgccac gctgtagatg aagcgagtgt cgggcagtgt
     7561 tcctcgttcg tagcggtaga gatacgggat gcgggtgtcc ccgccgaggt ccatgtcgtc
     7621 cgcaccgaca aggtcgctga cggcaaggta cgcgacgtcg gcgggcaacg tcgcgtccac
     7681 accgtcacca acattgagcg gcaccacctc accgtcggtg ttgcgatagc ggtactcgat
     7741 acgggtgtcg gcctggcagt ctttcgacca gccgagcggc tcctgggaac gatccggcgt
     7801 gtcgggctcg gactccgggt acaccggggt tcccctccgg tcctgattgt cgacgttcgg
     7861 ctggccgaga tcgttgtact cggtggtgca caggaacggc tcttgaacct ccacctcggc
     7921 ggagtcgtcg agcgccgatg caccggcggg aacgacggtc gtggccgtga cggccacgag
     7981 cgcggtcgcg gccaacagcg cctcgacgaa acgacgcgcc gctttcgtac cgcgaggtcg
     8041 gccagcgctg ttggcgcggt gaacggaaac caccaagtcg gtcacgtcga gatcctcccc
     8101 ctacgacgaa tgtcgattgt gatcgcgacc ctatcggcga tgccgtcgcc gacgacgctg
     8161 atctcactcg tcgctgtacg ccgactgccg gcatgcacaa cgacaccggt cttggcaccg
     8221 tcggaaatgg gccaggagcc ccagaggatt ctcagcagac acccttaccc tgagccacat
     8281 gaagcgaacc ctttgtctcc tcgccgccat ggcgctcgca ctcaccacta ccggatgcga
     8341 gaagttccca aactgcgacg cgcttcgcgt tcagttcccc cacggagttg ggttgcccgg
     8401 cgccatcgac tcaaccagcg ggtcgaatcc aatcaccgat tttcaggtgg aggagaccta
     8461 ctacaagttc aacagccatc tcgatcgcga caacgacggc atcgcctgcg aacaggcata
     8521 gccgcgtcgc gccggagttc gccctacgcc acgaacgcgt cggcacccga cgcgttgtag
     8581 ggcacgaaca cctcgcgggt ctcgcgatag cgcacgagct cgatcgccca gaacaccccg
     8641 aggccgcgca cctcgccgat cgaccgatgc cgctcaggcg tcgacgcgag ctccggcccg
     8701 atcacctcgc aaccaagccg acgctccgca cccaggatgt cctcgtctga atcagctcct
     8761 cacgctcacg gcgcggcgat cgcctcgagg acgtccaagc gtgcgacccg ccgagccggc
     8821 cggcgcgccg cggcgattcc ggcgagagcc cccaggaaca ggatcatcac caacctcgag
     8881 acggggaacg acacggtgcg aaggagttcg tctgatgatg acgtgagcac agcccacgca
     8941 gcgaacatcc cgacggccaa gccgatgatg gtgccgaacg tagcgatcat gatcgcctcg
     9001 agtcgcaccg tcgttcggac ctggcggcgg gtctgcccga gcgcccgcat caggccgatc
     9061 tctcgaaccc gttccatggt cgccagcgag atcgtggtgg cgattcccag cagggcgatg
     9121 ccaattgaga gcgcgagcaa cccgtacacc aacgccaaca tcgtgtcgat tcgtcgcccg
     9181 atcgtctcgg cgtagtcctc acggtcgagg gactcgacgc cgggagcgtc gctgatgagc
     9241 atgtcgatgc ggcggtcgag ttcgatcgcc gagacgcctt cgctggctcg caagaccacc
     9301 agcgatgtcg aaacgccggg cgagtgcgat ccggcggtgt cagctggcat gacatagtcc
     9361 ccggcgaacg ccgtgttgtc gtagagcgcc acgacgcgca actgcaactc gactccgtcg
     9421 aggaaccggg ttggcagcgc atctccgaca gcgacgtcgt gatccgacgc ccacgtggcc
     9481 gacacagcga tgacgtcgcc ggtggctccc gccaccgacc cgtcgatcac gccaaggtcg
     9541 aacatcgccg caccggcagc catgtcggtt gccgtgacaa aggccgtctc gcctccgaca
     9601 gttaccggca tgaacgacaa gggcagcgag gagtcgaccc catcactcga ggcgacctca
     9661 tccacaagcg catcgtcgag tcccgacccg ctgaagctcg tcggttgcac gatcacatca
     9721 ccggccatct gttcgtcgac actccgggtc accgcccggt tcagcgacgc tgcgacgacg
     9781 gtgaacagcg tgacgacggc gacgccgata acgagcgctg tgacggttga tgcggtgcgg
     9841 cgcgggtgac gaacggcgtt gcgttgggcg agcaatccgg cggtgccccg cgtgcgggcg
     9901 atgggcgcac cgatgaccct caccagggcc ggggccaggg ccggaccgat gaccacgacc
     9961 gacagcagcg tgatcgcggc cccgacgccg gcccgcgtcg atgcacccga attggcgctg
    10021 agcgcagcct gaacggcgag cccgactccc gcggccatca acgctgcgcc gaggatcgtc
    10081 cgccggcgcc ccacactgcc gccgtcgatc gccgcgctcc gcatggcctc gaccggtgcg
    10141 atacgtgagc cgcgacgcgc tggtgcgatg gcggcgatga ccgtcgagac gataccgaca
    10201 ccggcaccga tgatcaccga cgatgccgaa acgacgagat ggtcgccgag cgagaagaag
    10261 ccgacgcggc cgatgagcca actcagcatc ctggcgagga gtaggccgaa tcccagaccg
    10321 acagcggagc cgagcacccc gaccgcgaac gactctccga gcgtccaacc ggacagttgg
    10381 cgtcgggacg ctcccagggc acgaagcagc gccgactcgc gggtccgttg agcgacgagg
    10441 atggcgaagg tgttgtagat cccgaacgcc gcgacgacga gcgcgatgga cgcgaaggtc
    10501 aacaagaccg cagagacgat cgtcgagaag gtctcgctca ccttcgccgc ctcatcctgc
    10561 aactcttgcc ctgtcaccac ttccagcgat ccggccgcag cgctctcgtc aagcgcggtg
    10621 cgaaggcgtt ccacgagttc agattggctg attccatcga gcgcgacgac ggagatcgac
    10681 gcggcggtac ccggatcggc gaggtactcc gctgcggcat cgggagaaag cagcgttgcg
    10741 gtcatgggcc cggccgattc ggcggtgccg tacgctgcaa ttccgacaag gcgaagccgg
    10801 agccgtttgg ggaccagcac ctcgatcgtg tcgccgaccg acaggtcgcc ctgcctggcc
    10861 gacgcccggt cgatcacgac ctcgtcgagg ccctgcggtg cgcgcccctc ggcgatccga
    10921 aaggggttga gatcggggtc gtctcgccat tcaaacccga gttgtggcgg accgcttccg
    10981 ccgatcgtgt cgccatcgga tccgacgatc atcgcgggtc ggctgtacgc aggctcggcg
    11041 atgcgaaccc cgtcgacggc acgggcgatc tcggccagat ccgccgggac gtggccgcgg
    11101 taggtctcct cgttggttga gatcgaattc gccgagcgaa tgctcgcgtc gatcccggcg
    11161 accgaggatc gcatggtgtc gtcgaagctg cgcgagaagg tcgcgacctc gacaagtacg
    11221 gcgaacagaa aggcgattcc gaccgccacc gcgccgacgg tggcgacata gcggcgggga
    11281 tgtgcggtca gattgcgccg gatgatgatg agcattgttg gtcctggtcc ctggttcgtg
    11341 gaggtgaagc tgtggcgggc cgtcgaggat cagtcgccga accgcttcat tcgatcgagt
    11401 accgccgaag cgctcggcgc ctcgagttcg tcgacgacgt tgccgtccgc aagaaacacc
    11461 acgcggtcgg cgtacgccgc tgccccggga tcgtgggtca ccatgacgat ggtctggcca
    11521 acctcatcga ctgcgctgcg caacaggcgc agcacctcgt cgccgctgcc ggagtcgagg
    11581 ttgccggtgg gttcatctgc gaagatcacc tcgggtcggc cgacgaggct gcgcgctacc
    11641 gcgacgcgtt gttgctggcc gcccgacagt tcgctgggac ggtggccgag gcgcccttcc
    11701 aacccgacgc gccgtacgac ctcgtggtac cagctctcgt cgagccgggt gcgcgcgagc
    11761 atcgaaggca gccggatgtt ttccgcggcg gtgagcgtcg cgaccaggtt gaacgcctgg
    11821 aagatgaacc cgatgcggtc acggcgcaac tcggtcaacc ggcgctcgtc aagcccggaa
    11881 atgtcgatgc cggcgatctc aatcatgccc gacgtggggg tgtcaagccc cgcaaggcag
    11941 tgcatcagcg tcgatttacc tgatcccgac gggcccatga ttgccacgaa ggagcccgat
    12001 gcgacgctga gcgaaacgtt ggagagcgcg tcgagagctc cggggccgtc gccgtagcgc
    12061 ttggagagga tgtctgctcg aagcgccgtg tcggtggccg caacctccga aatcggggcg
    12121 cgcaaggctg aggtgttcat gatgctcctt gatggtctat ggctgaagtc ggcgcccggg
    12181 gaggcccgaa ccgaggtctc agctggcgag aatctgtgct gtcaccggtt ggcgacccgg
    12241 atgttgccgc cgcgtgctga gaggttgagt cgatgccggc tcgcaggatc gaccggtacc
    12301 agcaccgttg tcgagccgga cgagtcgatg cgataggacg tggcatcgga cgggaccgcg
    12361 agattgatac tgccgccagt gacagcggcg tcgacgctga gcggcgacgc gtcaaaggcc
    12421 atgtcgagcg ctccggcgac gagggttgca gagacctgct cgcttcggac gccggttgcc
    12481 tgcaccccgc cgccgaagat gtcgagctcg agcgggccgg aaacgttcga caacgatgcg
    12541 cctccggctc gaaccgtgat cgtgaccgag ccggaagtgt tgcgaacctg tacgtcgttc
    12601 ccgccgatgt cgacagcaac ccgttcaggg gcgacgatct cgacgtcacc gaaacagatc
    12661 cgaccggcca gccctgaaca acgcgactcg agatacaagg tggttccctc gacgtggtcg
    12721 tcgatcgtcg gttcagaaaa agactggtaa agcgtgcgcc gaaccgtgat ctcgtcgggc
    12781 gcaacggagt tgccgtcggg cgcggaggcg gccgggaact ccggcccgtc ggcggactcc
    12841 gctgatccgg acgcctcggc ggactcgatc gcgtcgggca cgcccgtgac cctcacccga
    12901 ccggcgttgt gcacgacgat gcgatcaatg gccgtcgcgt cgtagcgggc ggtcgaatct
    12961 gtggtgctgg cgaccaatcc cgtcgcgatg gcgaacgatg ctgtgcccac actgccgaac
    13021 gacagcaccg cgccgaccag aagcagcgcc ggacgcgcga gcttgtgcga actcgtcatg
    13081 aggtcacctc caagtaccgc aaaaccgcta gaacgcggcg atgatcggtg tcggccggtg
    13141 cgagcgagag cttggcgaag atgttggaca cgtgcttttc caccgcaccc tccgacacga
    13201 ccatcgccgc ggccaccgca cgattggaac atccctcggc catgagcgcg aggacctcgg
    13261 cctcccgctg cgtgagcgag tcgagcgggt tacggtgacg gctcgtcgac agcaactgcg
    13321 ccacgacctc ggggtcgagg gcggtcccgc cggtgccgac ccgccgcacg gcgtcgacga
    13381 actcggcgac gtcagcgaca cggtccttca acagatatcc gaccccgacg gtgctccccg
    13441 ccaacagttc ggtcgcgtag cgctcctcaa cccactggct cagcacgagc accgctatct
    13501 ccgggtgccg ggcgcggatc gcgatagcag cccgaagacc ctcgtcggtg tgggttgggg
    13561 gcatgcgcac atccatgacg acgatgtcgg ggcgataggc ctcgaccgca gcgagtccgg
    13621 actcggcatc acccgccgtc gccacgactt cgtcgcctgc gtcctgcaac aaccgggcga
    13681 taccgtcgcg gaggagaacg gagtcttcga cgatgacgat tctcatgacg acggcccttc
    13741 ggtgattcgg cgagcagatt cgagcgggga tgacgacggc tcgatcggtg gcggtggagt
    13801 cggcggcgac ggcggtggga cgggcaacac ggcccgtagc accgtcggac cgccgggggg
    13861 acttgaaacc gtgagcagac cgtcgaccgc ctcgacccgt tcgcgcaacc caagcaagcc
    13921 agaacgctgc gtcggctcca gcccgcgagc tgctggttcg ccgatgctcg gggcgccagc
    13981 agtcgggtcg ctggcgccac cgtgaccatc gtcggcgacc tcaacaacga ggtggtcctt
    14041 ccctgagcct ggagggcgca cctcgaacac gcgcaccgac gaacgggtcg caccgctgtg
    14101 tcgggcgacg ttcgtgagcg actccgagat cacaaagtag gcggtgctct cgatcgcccg
    14161 aggtggccgc acgtcgagct gaacatcgac ggcgacgggt accggacttc gagcggcgag
    14221 ccccccaacc gcggcggcga gaccccggtc ggtgagcacc ggcgggtgca ggcctcgcgc
    14281 aaggtcgcgc agttcggtga gcgctgcctt cgcttcgcca tgggcctcgt cgagcagcga
    14341 tcggagttcc tcgcgatcac cgtcaacctt ttggcgggcg agccccaggg tcatcgcgag
    14401 cgctaccagt cgctgttggg caccgtcgtg aaggtcgcgt tcgatccggc gccgctcaac
    14461 ctctgctgca tgaagcgctc cggcgcgggt ggtttcgagc gtgtcaacgc gctctcgcaa
    14521 ttcgagttcg atgaggtcgg tgctgatgat ctggcgagtc aacgacagca gcaccgtcga
    14581 caggccccta gtgagccagg gcgcgatcag gacgagcgcg aggccaactc cggctgcgac
    14641 ggtgtattcg acagcgccgt cgagcgacac ggacaagagg tttccagctc caccaggcga
    14701 ccgtggcgtg tagagaggca cgaggacgag cgcaatgcca acggcccaca cggcgacggc
    14761 gatcgtgaag aacagccacg aaaccggcgc atgaaggagg tggaagatca actccttcca
    14821 cgtccccgtt tgtttgaggt aggcgacgcc gcggtctgtg gcttgacgca gggtcgacgg
    14881 tggcgatggc gcggcatcgc cccccgtccc aggagtcatg gtggcgggag tcatggtggc
    14941 gggagtcatg gtggcgggag gatgcaccgc tgcgcgggga ggatgtgggc tcggcagaga
    15001 ttcgttgagc gccgtcgccg cgatccagcg ggtcacgcgt gccaacccgt tcgagaaggc
    15061 gaacgcgaac gcggcgaggg cgatggcggc cggcgggaag aacacggtgg cgaccgttgc
    15121 ggtcacgagc gcgaccatca ccgatgtcgt caccaacgac cacatggcat cgacggcgag
    15181 gtaggcgaat gccacccagg tgctgggtgc gaacggggcg agcagcggac gaacgaccag
    15241 agacgcccga ccgatggcac cgggttgcga ggcatccggg tgattcgagg tcaccggagc
    15301 cggggcgg
//
LOCUS       DAOOMA010000003        15009 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_4233_length_15009_cov_4.472516, whole genome shotgun sequence.
ACCESSION   DAOOMA010000003 DAOOMA010000000
VERSION     DAOOMA010000003.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 15009)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15009)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15009
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4233_length_15009_cov_4.472516"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..827
                     /locus_tag="PLP95_00165"
     CDS             <1..827
                     /locus_tag="PLP95_00165"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HPB44249.1"
                     /translation="IIPVGGVDDDLFAQLDALAAQTYDRPWELVLSMNQPGQARDLRI
                     SEHALHPLATLVMVDSSAIRSAAHARNSGATAATAPMLAFCDADDICEPTWLSEITAA
                     MADHDAVGGHLDEERLSPPGQEKWRPPATPGDCPPFLGHRYPVSANMAVTRSAFDAVD
                     GFPEGFTRCEDIAFGWALESAGINLGFAERAVVHYRHRPGLWTMIRQHHFYGIGMSEV
                     LIRQGLPTSDDGASPPSVFQANSQRVTQRSFIHILRRGAIASGRLRGLVQERLRRS"
     gene            904..2547
                     /locus_tag="PLP95_00170"
     CDS             904..2547
                     /locus_tag="PLP95_00170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB44250.1"
                     /translation="MEWSFISAAVRRRLGLVIALAVLGALPGFLGLVSSGDSYQATAT
                     INVAAPTGNGAAAAANQPDRWVLSQLSVVQSIDTLDDAAATLGSDWTGAGIGSLINVE
                     QEPETDLIHVTATAGDASTAALVANAVVDTYITESANRLREARQPAIDELQKRLDDIT
                     ARLNGRAASEGVEAIVGINPQIADAMKGYIANASNPSYVIPQDSQIVPQLVQQRDQLL
                     VEGDRIRSAISDIQLSAVSEATSSVIERATEPDGTTAGLGRYLTVAGLIFGGLVGTAL
                     ALVIAQFSSKVLDEQVAENILRTNIVDSMPRSRAYAASPLRALTTSPPEMVETIERIG
                     IRAEAMASSEIDQALTIAVLGTQPDSGTTTLTLALARRFAQVGYSVIVIDGDLNTRTI
                     STLVEAEPNGGIKAVLRQTARNRPITDSVFTSTDLDKVRVLGTGSIDAHSSLRRDALG
                     PILEAARSRAQVVLFDGGSLTGSSVSVFAARAADVVVLAVPLQSQDQRTLTEAAGLLP
                     DDRSRVLAVVTAPGRRRVHEAPLPTVDDSDDSDELADVS"
     gene            2547..3644
                     /locus_tag="PLP95_00175"
     CDS             2547..3644
                     /locus_tag="PLP95_00175"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HPB44251.1"
                     /translation="MTERKLGYRPGLDGIRSVAIGLVLLNHTGLSIFEGGGNGVILFF
                     VLSGFLITKLMIEEWGRTDRVSIGGFYGRRAVRILPAPTVMLITLFLLRDVITDTVET
                     RAYLMKELLLSATYTMNMRPLLAPYQPVIDGEPFLGYLGHMWSLAVEEQFYLVWPLLM
                     VSLLLPFASRLGVRAGVPLKLTIAAATITVVRLCIYFLPFVNSDIASIAVFSFDGFAL
                     GAALAFALHYGSAPRLESLLAQSWVPWAALAVLFADLLAGRWTHDVTSVYVAYTSIAS
                     AALIGHIMVAPDSMLSKAAAMALPVYIGKLSYSIYLWHNPIWVYVSSSRFDLPLVTLT
                     LIEWSATAVIVLISYYGIEKPALKLRHRFVR"
     gene            complement(3673..4203)
                     /locus_tag="PLP95_00180"
     CDS             complement(3673..4203)
                     /locus_tag="PLP95_00180"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HPB44252.1"
                     /translation="MTAAVASDVALAVRRVSSPIGELTIVASDAGVRAVLFAQESLSA
                     IDIGNGVPSRQAIRHAHRAAEQLQEYFDKQRRDFEVDLDVLGTEFQRRVWSALSEIPY
                     GETISYAEQALRVADRGAARAVGAANGRNPVPIIVPCHRVVGSDGTLTGYAGGTDAKA
                     FLLRHEQWRQPSLFDL"
     gene            complement(4223..4675)
                     /locus_tag="PLP95_00185"
     CDS             complement(4223..4675)
                     /locus_tag="PLP95_00185"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HPB44253.1"
                     /translation="MTSVSNEQPVVIVGVDGSPNSLAALEWAINFAAGQGGGTVKAVM
                     SWTYPAAAVGSMGIGGVMPPAESMHEASAEALDEILADVVAPDSVTVQPVIAEGGASP
                     AILELADDADLIVIGKRGHGGFLGLLIGSVATQIVNHAPCPVVVVPAG"
     gene            complement(4724..5911)
                     /locus_tag="PLP95_00190"
     CDS             complement(4724..5911)
                     /locus_tag="PLP95_00190"
                     /inference="COORDINATES: protein motif:HMM:NF013126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HPB44254.1"
                     /translation="MVHAVEPSSPRRFMAIGVAAVLVVSGCVGATRVSVEAGSTSDAE
                     TVTTLLAEVQPAVDVPACVGELTVEQKAAEMLMVLVDTPGRAAPLVSQRLVGGYGLVG
                     DQAAEVAGQVAMVGSVATFPLLVSSDEEGGTVQRLKAALGELPAARSVAESMSVGDAT
                     LLYRNFAAKMKAAGFSMNFAPVLDIGTGAELGTRTFGNDATTVKNYGLAAMQAMTAAG
                     VIPVVKHWPGLGTGGADPHKAAVPLGTFEELRTNDLVPFEQAIALDAPAIMVSHGSIS
                     QSTGGLPASISREAITELLRGDQRFDGLVITDSLGMSAVTMHVPESQAAMMAISAGAD
                     IALVSKPEEVQPAHDAITGAISGGLIDAQQVDASVARVLRAKGVTGACPALAAPPAGD
                     GGG"
     gene            6009..8195
                     /locus_tag="PLP95_00195"
     CDS             6009..8195
                     /locus_tag="PLP95_00195"
                     /inference="COORDINATES: protein motif:HMM:NF040071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6351 family protein"
                     /protein_id="HPB44255.1"
                     /translation="MTIHLPVPHRRGLAAVLAGALLFTGCSSSNDAAAPTTAERSEHG
                     FRVDGVSTALTRVTGGDVVVAVEGTAEVATFQLDGAALDTSVVAPGSGTLIDDENRTL
                     YLISGLDEGDNDVTVRAGDESQSITIVNHPISGPVFSGPHLPMPTCTTDDFGLGPATG
                     EDCFAEPSLRRAYFTADRTLSDLGADEVVPSDAYRDERGNAVEVEVETGVINRGVYEI
                     MTPVSTEWNGRLVYRFGGGCGVTYSQGFIMANDPDLELFERGYAVATSTLNTFQVTCN
                     DVISAETAMMVKEHFVESHGEIAVTIGEGGSGGAIQQLLITQNYPGILDAIAPTMPFP
                     DAVTISGGVLDCALLNDYYRRAGAELTAQQHTAINGHMTEGTCSFWEETFAPAVNPTT
                     CGFGDIVNSAAAAIPGLGDAEFPRPPAEAIWNADSNPDGVRCTLQDGNVNELGIDDDT
                     GFARRPWDNVGVQYGLSALNEGAIDVDEFLDLNSGVGSFDINGNIVASRAAIDAETAA
                     VAYRSGRVLGSLQPHMMAVPTILVNIYTDPTGDIHDRFRMFSIDERLQLDGHQAPNVA
                     LWTRSVPPGAGLVESLSGSIGFGIQLVSTLDEWATALKSSSKQVDAAALAASRPAAAV
                     NSCFAADATLVDSGDDIYDVAGPCRDDFQTFGDSRIVAGAPLRSDIVKCSLQTLDSAT
                     TAGVWAVEFTDEQRKRLGEVFPDGVCDWSAPGVGQEPNDGPWQDFS"
     gene            complement(8188..9948)
                     /locus_tag="PLP95_00200"
     CDS             complement(8188..9948)
                     /locus_tag="PLP95_00200"
                     /inference="COORDINATES: protein motif:HMM:NF038032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CehA/McbA family metallohydrolase"
                     /protein_id="HPB44256.1"
                     /translation="MARFLKQFAVIGAAVAALGAGLAGGTVVPVSVAGAQESTAPTCA
                     ADGSVQTMTGTVSADQAKTYLLLPFEVPEGATRVEVGYSWAPSEGTTLDLGLWDASGT
                     SGPDAFRGWGGSRQGRLDKGMPRAWVSAETADRAFTPGDIEPGTWNVELGLAEIAPEG
                     ATYTVEISCSDGPAGRETPADPVDADHVANPDPGWYRGDFHMHSWNSLPEAPDYEGTV
                     QYGRDAQLDIMPITEYVVTRHHRELGAVQRANPDVLIWPGREVITYFGHAIVLGETPN
                     TIEYREGFDGVTLRGIQRDSIADGALFGVAHPTVFPEAEFGSLCRGCEFQLSDDIDWD
                     AVTTLELLTNTPVVGDVENPFIQTAIDYWQGLLDAGHRITAVSGSDDKRGPALGSTAT
                     MVYAKELSRGAVTEALEAGHTYIQVRGAADSPTVEFTGASGGAEAMMGDSLRADSVSF
                     SATVRDGDGQTLHIIRNGAEVEAVPVVGSEFTYTWDAERGEDGGGLGTTVRIEVSDAT
                     SRTVITNPIFLVDHEPSPTTEAVSPTTEVAAPEPLGNAAGDDGSPAALIAGIAVALVA
                     LVVGGVAVGRRGRSGTSGVS"
     gene            complement(9958..10923)
                     /locus_tag="PLP95_00205"
     CDS             complement(9958..10923)
                     /locus_tag="PLP95_00205"
                     /inference="COORDINATES: protein motif:HMM:NF014510.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diadenylate cyclase"
                     /protein_id="HPB44257.1"
                     /translation="MVSWSDPPSPRLRRLVDELDEHGVDLDVENPLDRLVAEEIDYAL
                     HPLIHERRVPTYGAVIAPTVDRSRWRKTTNLEVTSRTLRDLQRSDARRFADGASSWLV
                     LSGDGAQKLAVFDRPASSERDVVVMVDAFGAAIVQRHSSGTVRYASEHGVMRWDGMSW
                     HREPLVTRWISAMRTWGGNAQHSLATLLAFAVHDLGARGIGALLIMQNTLTSSPAFEQ
                     RMAEPPPLDIAVPEDLTPLRHVLAQIDGAAVFDNSGILRQIGVRLNPSAAAVADVDGH
                     RGMRHTSGRRYSFDDPESIVIVVSEDGPVTMMRNGVILGRTDAQA"
     gene            11123..12061
                     /locus_tag="PLP95_00210"
     CDS             11123..12061
                     /locus_tag="PLP95_00210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015439938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HPB44258.1"
                     /translation="MAKQRPERDEEDLVRLYLSDIGQYPLLTKDGEVELAKAIEAGTA
                     AKAEMEAADGSLTAARKRELRRLIRTGEEAELAFVQSNLRLVVSIAKKYQASGLPLLD
                     LIQEGNLGLIHAVEKFDWRKGFKFSTYATWWIRQAITRGIANTGRTIRLPVHAGDVLS
                     RLQKARFRLETTLGRPATLLELAEELDMPVAKVTEAMRYAADTLSLSEPLRADGDAEL
                     GDMIEDRGAESPFDAAAVALLPEQINRLLGPLDERERQILKLRFGLDRGEPRTLEEVG
                     EHFNLTRERIRQIEARAMSKLRHPSSDTGAKDLLAV"
     gene            12211..12711
                     /locus_tag="PLP95_00215"
     CDS             12211..12711
                     /locus_tag="PLP95_00215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB44259.1"
                     /translation="MNSFTDLRQDDDDTDEADDFLQRLSELDQFVGAPDATFSPAIDH
                     LGDSVSSGDSGSDEASDFLERLDDLDRFVSAQRIDLDASDEAEEFLERLGDLDRFMPA
                     DATAEHSQAAFCPRCRRPAAPGDPEVVRVVHILGTVGGRPVDGQPAFMHRSCRVDNDS
                     EFREIH"
     gene            12766..12839
                     /locus_tag="PLP95_00220"
     tRNA            12766..12839
                     /locus_tag="PLP95_00220"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:12800..12802,aa:Arg,seq:cct)
     gene            12904..13314
                     /locus_tag="PLP95_00225"
     CDS             12904..13314
                     /locus_tag="PLP95_00225"
                     /inference="COORDINATES: protein motif:HMM:NF020839.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HPB44260.1"
                     /translation="MTHHDTTARLHQIGAVEDQVGLSQRTIRHYDEVGLVTPSGRSPG
                     GFRLYTDDDVARLKHIKAMKPLGFTLEESGDLLRIRDQLERGEALSPAELDYLTASLS
                     RAEERCAKLARQLDEAKALTATMRAEIDSAKLRP"
     gene            13601..14254
                     /locus_tag="PLP95_00230"
     CDS             13601..14254
                     /locus_tag="PLP95_00230"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HPB44261.1"
                     /translation="MTGGSSGIGAELCKELVSRGASVTSVDVTPPTCDVAGITHLDGD
                     VRTFDFGSLAPIDLIVANAGVVDSTRHRPLTEDAVRRLIDINLVGVARTLTAPTTPSG
                     RALIISSRSAVAPEAPGALYGATKAAALNYGLSAGSVRPTTVALVGITATNLFAAEAD
                     YQGRAVDPPRSTFGDPSEVARACLDAVEAGDPLVITDRDTLRDVSAFGFAFLTARLR"
     gene            14574..>15009
                     /locus_tag="PLP95_00235"
     CDS             14574..>15009
                     /locus_tag="PLP95_00235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase dimerization
                     domain-containing protein"
                     /protein_id="HPB44262.1"
                     /translation="MTDQYLADAFNLVAHRNPGESEFHQAVKEVLDTLEVVLKKHPEM
                     AEQRLIERVCEPERQIIFRVPWVDDEGCIQINRGFRVEFNSALGPFKGGLRFHPSVNV
                     GIIKFLGFEQTFKNALTGMPIGGGKGGSDFDPKGKSDGEIMRF"
BASE COUNT         2670 a         5230 c         4547 g         2562 t
ORIGIN      
        1 tcatcatccc ggtcggtgga gtcgacgacg acctgttcgc ccaactcgat gccctcgctg
       61 cccagaccta cgaccgtccc tgggaactcg ttctgtcgat gaatcaacct gggcaggctc
      121 gggacctccg catcagcgag catgctcttc acccgctcgc cacgctggtg atggtcgatt
      181 cctcggcgat acgaagtgcg gcacacgcgc gcaactccgg agcgacggcc gcgaccgccc
      241 cgatgctggc cttctgtgat gccgacgaca tctgcgagcc gacgtggttg agcgagatca
      301 ccgcagcgat ggccgaccac gacgctgtgg gtggccatct cgacgaggag cgcctgtccc
      361 ctcctggtca agagaaatgg cgtccccccg ctactccggg cgactgtcca ccgtttctcg
      421 ggcaccggta tccggtgtcg gcgaacatgg cggtgacccg ttcggccttc gatgccgtcg
      481 acggatttcc ggaagggttc acacggtgcg aggacatcgc gttcggatgg gcgctcgaat
      541 ccgccgggat caaccttggc ttcgctgaac gagcggtcgt tcactaccgc catcgccccg
      601 gtctgtggac catgattcgt caacatcatt tctatggaat cggcatgagc gaggtgctga
      661 ttcgccaggg acttcccacg tccgacgacg gcgcttcgcc tccctcggtt tttcaagcga
      721 acagtcaacg cgtgacacaa cgctcattca tccacatact gcgtcgaggc gctatcgcgt
      781 cgggacgact ccgcgggctc gtccaagaac gcctccgtcg ctcctgattc tctctttcca
      841 cccacattca tctccccttg ggcacactgg attgccccga tcttctgcct gaaaggctga
      901 gcaatggaat ggtcattcat ttctgcagcg gtgcgacgac gcctcggact ggtcatcgca
      961 cttgcggtcc tcggagcact gcccgggttc ctcgggctgg tgtcctccgg tgactcctac
     1021 caggcgaccg cgacgatcaa tgtcgccgcc ccgaccggca acggtgccgc agccgcggcc
     1081 aaccaacccg accggtgggt gctgagccag ttgtcggtgg tgcagagcat cgacaccctc
     1141 gacgatgcgg cggccacact cggcagcgac tggaccgggg caggaatcgg ctcgctgatc
     1201 aacgtcgaac aggagccgga aaccgacctg attcatgtga cggcgaccgc aggcgatgca
     1261 tccaccgcag cgctcgtcgc caacgcggtc gtcgacacct acatcaccga atccgccaat
     1321 cgccttcgcg aggcccgtca accggcaatc gacgagctgc aaaagcgact cgacgacatc
     1381 accgcgaggc tcaacgggcg tgcggcttcc gaaggggtcg aagccatcgt gggtatcaac
     1441 ccgcagatcg ccgacgcgat gaagggttac atcgcgaatg cgtccaatcc cagctacgtc
     1501 attccccagg actcccagat cgtcccccaa ctcgtccaac agcgcgacca actcctggtc
     1561 gaaggcgacc ggattcgctc ggcgatcagc gacatccaac tcagcgcggt cagcgaggcg
     1621 acgagcagtg tcatcgagcg agccaccgaa cccgatggga ccaccgccgg tctcggccgc
     1681 tacctcaccg ttgccggctt gatcttcggc ggcctggtcg gcaccgcgct agcactcgtg
     1741 atcgcccagt tctcctcgaa ggtgctcgac gagcaggtcg cggagaacat cttgcgaacc
     1801 aacatcgtcg actcgatgcc acgcagccgc gcctacgccg catcgccgct gcgggccctc
     1861 acgacgagtc cgccggagat ggtcgaaacg atcgagcgca tcggcatccg tgccgaagcc
     1921 atggccagct ccgagatcga tcaggcgctc acgattgccg tgctcggcac gcagcccgac
     1981 tccggcacca caacgctgac cctcgctctg gcgcgtcgat tcgcccaggt cggctactcg
     2041 gtcatcgtga tcgacggcga cctgaacacc cgcaccatct caacccttgt cgaggcggag
     2101 cccaacggtg gcatcaaggc cgtcctgagg cagacggcga ggaatcgccc catcaccgac
     2161 tcggtgttca cctcgaccga cctcgacaag gtccgggttc tcggcaccgg cagcatcgac
     2221 gcccactcgt cgctgcggcg cgacgcgctc ggcccgattc tcgaagcggc acgctcccgc
     2281 gcccaggtcg tgttgttcga cggagggtcg ctcaccggat catcggtgag tgtgttcgcc
     2341 gcccgagcgg ccgatgtggt ggtcttggca gttccgctgc agagccagga tcaacgcaca
     2401 ctcaccgaag ccgccggact gttgcctgac gaccgctccc gggtcctcgc cgtggtgacc
     2461 gcccccgggc gccgccgggt gcacgaggcc ccgctgccga cggtggacga ttccgacgat
     2521 tccgacgaac tcgcggacgt cagctgatga ccgaacgaaa actcgggtat cgcccgggac
     2581 tcgacggaat ccgatcggtg gcgatcggcc tggtcctgtt gaaccacacc ggtctgtcca
     2641 tcttcgaggg cggcggcaac ggcgtcattc tgttcttcgt cctcagcgga ttcctgatca
     2701 ccaaattgat gatcgaggaa tggggacgca ccgatcgcgt ctcgatcggc ggcttctacg
     2761 gtcgtcgggc ggtgcgaatc ctgcccgccc cgaccgtcat gttgatcacg ctgttcttgt
     2821 tgcgcgacgt catcaccgac accgtcgaga cgcgggcgta cttgatgaaa gagttgttgc
     2881 tgtcggcgac ctacaccatg aacatgcgcc cgctgttggc gccgtaccag ccggtgatcg
     2941 acggcgaacc gttcctcggc tacctcggcc acatgtggag cctcgccgtc gaggagcagt
     3001 tctacctggt ctggccgttg ttgatggtgt cgttgttgtt gccgtttgca tctcgcctcg
     3061 gagttcgggc gggggtcccc ctcaagctga cgatcgcagc cgcaacgatc accgtcgtgc
     3121 gcctgtgcat ctacttcctg ccgttcgtca actccgacat cgcctcgatc gccgtgttca
     3181 gcttcgacgg cttcgcactc ggcgcggcgc tggcgttcgc tctccactac ggcagcgcac
     3241 cccgtctgga gtcgctgctc gctcagtcct gggtgccgtg ggccgcgctc gcggtgctgt
     3301 tcgccgacct tctcgcaggc cggtggaccc acgatgtgac ctcggtgtat gtggcctaca
     3361 cctcgatcgc gtcggctgcg ctcatcggcc acatcatggt cgcgccagac tcgatgctgt
     3421 cgaaggccgc tgcgatggca ctgccggtgt acatcggcaa gttgtcgtac tcgatctacc
     3481 tgtggcacaa cccgatctgg gtgtacgtca gttcgagccg attcgacctg ccgctcgtca
     3541 cgctgactct catcgaatgg tcggccaccg ccgtcatcgt gctcatctcg tactacggca
     3601 tcgagaaacc agcgttgaag cttcggcacc gtttcgtgcg gtagcgcccc aggccgacca
     3661 gtggcccagg cctcagagat cgaacagcga tggttgacgc cactgctcgt gacgcaacag
     3721 gaaggccttt gcatctgtcc cgcccgcgta cccggtgagc gtcccgtccg acccgaccac
     3781 ccggtggcac ggcacgatga tcggcaccgg gtttctgccg ttcgcggcac cgacggctcg
     3841 ggcggcacct cgatcggcga cccgcagcgc ctgctctgcg tagctgatcg tctcgccata
     3901 gggaatctcg gacaaggctg accacacccg acgctggaac tcggtgccca acacatcgag
     3961 atcgacctcg aagtcgcgtc gctgcttgtc gaagtactcc tgcagctgtt cggccgcccg
     4021 gtgggcgtgc cgaatcgcct gtcgcgatgg aactccgttg ccgatatcga ttgcggacaa
     4081 cgactcctgc gcgaacaaca ccgcgcgaac ccccgcatcg ctggccacga tggtgagttc
     4141 cccaatcggg gaactcaccc ggcgaacggc gagggcgacg tcggaagcca ccgctgcggt
     4201 catggggttg gcgttgaacg ggtcagccgg ccggaacgac cacgaccgga cacggagcgt
     4261 gattcacgat ctgcgttgcg accgagccga tcaggagacc aaggaacccg ccgtggccgc
     4321 gctttccgat cacgatgagg tcggcgtcgt cggcgagctc aagaatcgcc ggcgaggctc
     4381 cgccctcggc gatgaccggt tggaccgtga ccgaatccgg agcaacgacg tcggcgagga
     4441 tctcatcgag cgcctcagcg gaggcctcgt gcatcgactc cgccggaggc atcactccac
     4501 cgatgcccat cgagccgacc gcggccgcgg ggtaggtcca gctcatcacg gccttgaccg
     4561 tgcctccgcc ctgaccagcg gcgaagttga tcgcccactc aagcgccgca agcgagttcg
     4621 gagaaccgtc gacgccgacg atcaccaccg gttgttcatt cgatactgaa gtcatgccca
     4681 catcgtcgca cgtcggccgc ggcgccgcag gttcgccgac aagtcatccc ccaccatcgc
     4741 ctgcgggagg tgccgccaag gcgggacagg cgccggtcac acccttcgct cgaaggacgc
     4801 gggcaacgga agcatcgacc tgctgagcgt cgatgagccc gccgctgatg gctccggtga
     4861 tcgcatcatg ggccggctga acctcttccg gcttcgacac cagcgctatg tcggcaccgg
     4921 cactgatcgc catcatcgcg gcctgcgact cgggaacgtg catggtcacc gcgctcatcc
     4981 ccagcgaatc ggtgatgacg agaccgtcga acctttgatc tccacgcaac aactcggtga
     5041 tcgcctcgcg ggagatcgaa gcaggaaggc cgccggtcga ctgcgagatg gagccgtggc
     5101 tgaccatgat cgccggcgca tcgagcgcga tggcctgctc gaacggaacc aggtcgttcg
     5161 ttcgcaactc ttcgaaggtc cccagcggta ctgctgcctt gtgcgggtcg gctcccccgg
     5221 ttccgagacc gggccagtgt ttgacgaccg gaatgacacc ggccgcggtc attgcctgca
     5281 tcgcggccag cccgtagttc ttgaccgtgg tcgcgtcgtt gccgaaggtt cgcgtgccca
     5341 actcagcgcc ggtaccgatg tcgagcaccg gcgcaaaatt catggagaac ccggccgcct
     5401 tcatcttcgc cgcgaagttg cgatacagca gcgtcgcgtc tccaaccgac atcgactccg
     5461 caaccgagcg tgccgcgggc agttcaccga gcgccgcctt caatcgctgt acggtgccgc
     5521 cttcttcatc gctggaaacc aagaggggaa acgtcgcaac cgaccccacc atcgcaacct
     5581 gacccgcgac ttcggccgcc tgatcaccca ccagcccgta gccgccgaca aggcgttgag
     5641 acaccagtgg agcggcacgt cccggcgtgt ccacaagcac catcaacatc tcggcggcct
     5701 tttgttcgac ggtcaactcc ccgacacacg caggcacgtc aaccgccggc tgcacctcag
     5761 cgaggagggt ggtgaccgtt tcggcatcag aggtggaccc ggcctcgaca ctcacccgag
     5821 tggcccccac acatccactc acgactaaca cggccgcgac tccgatcgcc atgaagcggc
     5881 gggggctgga tggctcgacg gcatgcacca cggcgaacac ggtaccggtc gacgccgccg
     5941 gtcgtcctct cgccgttcag acctctcgtg tgcataggct caggcgccac cgaactggag
     6001 aaaccgacgt gacgatccac ctgccagtac cacatcgacg gggcctcgcc gccgtgttgg
     6061 cgggcgccct cttgttcacc gggtgcagtt cctccaacga cgcagcggct ccgactaccg
     6121 ccgagcggtc ggagcacggg ttccgcgtcg acggcgtgtc gaccgcgttg acccgcgtca
     6181 ccggcggcga cgtcgtggtc gcggtcgagg gcacggccga agtcgcgacc ttccaactcg
     6241 acggcgctgc gctcgacacg tctgtggtcg ccccgggcag cggaacgctg atcgacgacg
     6301 agaaccgaac gctgtatctg atcagtggcc tcgacgaggg ggacaacgac gtcacggttc
     6361 gcgcggggga cgaatcgcaa tcgatcacga tcgtgaatca tccgatctcc ggaccggtgt
     6421 tttccgggcc gcatctcccg atgcccacct gcacgaccga cgacttcggc ctcggccccg
     6481 ccaccggcga ggactgcttc gccgaaccct cgctccgacg cgcctatttc accgctgacc
     6541 ggaccctgtc cgaccttgga gccgacgagg tggtgccctc agacgcctac cgcgacgagc
     6601 gtggcaacgc ggtggaggtc gaggtggaaa ccggcgtgat caaccgcggc gtctacgaga
     6661 tcatgacgcc ggtctccacc gaatggaacg ggcggctggt gtaccggttc ggcggcggct
     6721 gtggggtcac ctacagccaa gggttcatca tggccaacga tcccgacctc gagctctttg
     6781 agcgcggcta tgccgtcgcc acctccaccc tcaacacctt ccaggtcacc tgcaacgacg
     6841 tcatcagcgc cgagacggcg atgatggtga aagagcactt cgtggaatcc cacggagaga
     6901 tcgcggtcac catcggcgaa ggcggctcgg gcggagcgat ccaacaactc ctgatcaccc
     6961 agaactatcc aggcattctc gacgcgatcg cccccaccat gccgttcccg gatgcggtca
     7021 cgatcagcgg cggagtgctc gactgtgccc tgctgaacga ctactaccgc cgcgctggcg
     7081 cagaactgac cgcccaacaa cacaccgcca tcaacggaca catgaccgaa ggaacgtgct
     7141 cgttctggga ggagaccttt gcccctgcgg tcaaccccac cacctgcggc ttcggcgaca
     7201 ttgtcaacag tgccgccgct gcgatccccg ggctcggcga cgctgagttt ccgaggccac
     7261 ctgccgaggc gatctggaac gccgactcca accccgacgg ggtgcgctgc acgcttcagg
     7321 acggcaacgt caacgaactc gggatcgacg atgacacggg ctttgcccga cggccatggg
     7381 acaacgtcgg cgtgcaatac gggttgtcgg cgctcaatga gggcgcgatc gatgtcgacg
     7441 agtttctcga cctcaactcg ggcgtcggca gtttcgatat caacggcaac atcgtcgcgt
     7501 cacgcgctgc gatcgatgcc gagaccgccg ccgtggcgta ccgcagcggc cgcgtgctcg
     7561 gttcgcttca accgcacatg atggccgttc ccacgatcct ggtgaacatc tacaccgacc
     7621 ccaccggcga catccatgac cggtttcgca tgttcagcat cgatgagcga cttcaactcg
     7681 acggccacca ggcaccgaac gtcgcgttgt ggacccgatc ggtgccgccg ggggccggac
     7741 tcgtcgagtc gttgtcgggg tcgatcggct tcgggattca actcgtctcg accctcgacg
     7801 agtgggcgac ggcgctcaag tcctcctcga agcaggttga cgcagcggcg ctcgcggcgt
     7861 cgcgtccggc cgcggcggtg aactcctgtt tcgccgccga cgcaacgttg gtcgacagcg
     7921 gcgacgacat ctacgacgtc gcgggtccgt gccgtgatga tttccagact ttcggcgaca
     7981 gtcggatcgt cgcaggagct cccctgcggt cagacatcgt gaaatgcagc cttcagaccc
     8041 tcgacagcgc gacgacggct ggcgtctggg ccgtcgagtt caccgacgag caacgcaagc
     8101 gcctaggcga ggtcttcccg gatggggtct gcgattggtc tgcgccgggc gtcggccagg
     8161 aaccgaacga cggaccctgg caggacttca gctgacaccc gacgtgccgc ttcgaccgcg
     8221 gcgacccacg gcaacgccgc cgaccacgag cgcgaccagt gccactgcga tcccggcgat
     8281 gagggcggcc gggctgccgt catcgccagc ggcgttcccg aggggctcgg gtgcagccac
     8341 ctcggtcgtg ggcgaaacag cttcggtggt cggactcggt tcgtggtcga cgaggaagat
     8401 cggattggtg atgaccgtgc gggaggtcgc atcgctcacc tcgatgcgca ccgtcgttcc
     8461 gagaccccca ccgtcctcgc cccgctcggc atcccacgtg taggtgaact cggacccgac
     8521 gaccggcacg gcctcgacct cggctccgtt gcggatgatg tgcagcgtct ggccgtctcc
     8581 gtcgcgaacc gtggcgctga aactcacaga gtcggctcgg agcgaatcgc ccatcatcgc
     8641 ctcggccccg ccggatgccc cggtgaattc caccgtgggc gagtcggcgg cgcctcgaac
     8701 ctggatgtag gtgtgccccg cctcgagtgc ctcggtcacc gctcctcgcg acaactcctt
     8761 cgcatagacc atcgttgcgg tcgaaccgag ggcggggccg cgcttgtcgt cggatccgct
     8821 gaccgccgtg atccggtgcc cggcgtccaa caggccttgc cagtagtcga tcgccgtctg
     8881 gatgaacggg ttctcgacgt cacccacgac cggcgtgttg gtcaagagtt ccaacgtcgt
     8941 caccgcatcc cagtcgatgt cgtcgctcag ttgaaactca cacccgcgac acaggctgcc
     9001 gaactccgct tcggggaaca ccgtgggatg cgccacgccg aacaacgccc cgtcggcgat
     9061 cgagtcgcgc tggatgccgc gtagcgtgac accgtcgaac ccctcccggt attcgatcgt
     9121 gttcggcgtc tctcccaaca cgatggcgtg tccgaaatag gtgatgacct ccctgcccgg
     9181 ccagatcaac acatccgggt tcgcccgctg taccgctccg agttcccgat ggtggcgggt
     9241 caccacgtat tcggtgatcg gcatgatgtc gagttgcgca tcgcggccgt attgcaccgt
     9301 cccctcgtag tcgggggcct ctggcaacga gttccacgag tgcatgtgga aatcgccgcg
     9361 gtaccagccc gggtcggggt tggcgacatg gtccgcgtcg acggggtcgg caggcgtttc
     9421 gcgccccgcc ggaccatcgc tgcacgagat ctcgaccgtg tacgtggcgc cctcgggtgc
     9481 gatttccgcc aagccgagtt ccacgttcca cgttcccggc tcgatatcgc caggggtgaa
     9541 cgcccgatcg gccgtctcgg cggagaccca cgcccgcggc atgcccttgt cgagccgacc
     9601 ctgacgcgag ccaccccagc cgcgaaacgc atcgggcccg gaggtcccgg aggcatccca
     9661 caacccaaga tcgagcgtcg tgccctccga cggggcccac gagtagccga cctcgacgcg
     9721 ggttgcgccc tcgggaacct cgaacgggag gagcagatag gtcttcgcct gatcggcact
     9781 caccgttccg gtcatcgtct gcaccgaacc gtcggcagcg caggtcggag ccgtcgactc
     9841 ctgcgccccg gccacggaga ccggaacgac cgtaccgccg gccaacccgg ccccaagggc
     9901 cgctaccgcc gccccgatca ccgcgaattg tttcaagaat cgagccactc gggcagccta
     9961 tgcctgtgcg tcggttcgcc ccaggatcac cccgtttcgc atcatcgtga ccggaccgtc
    10021 ctcactcacg acgatgacga tcgactcggg atcgtcgaac gagtaccgac gaccggaggt
    10081 gtggcgcatg ccgcggtgcc cgtcgacgtc ggcaaccgcc gccgcactcg ggttcaaacg
    10141 aacgccgatc tgacgcagaa tccccgaatt gtcgaatacc gccgcaccgt cgatctgggc
    10201 gaggacgtga cgtaacggcg tcagatcctc gggaaccgca atgtcgagcg ggggcggttc
    10261 ggccatccgc tgttcgaacg ccgggctgct cgtgagcgtg ttctgcatga tcaacagtgc
    10321 gccgatgccg cgcgccccga ggtcgtgtac cgcaaaggcc agcagcgtgg ccagggagtg
    10381 ttgagcgttt ccaccccagg tgcgcatcgc cgaaatccac cgcgtcacga gcggctctcg
    10441 gtgccaactc atcccgtccc aacgcatgac accgtgctcg ctcgcatagc ggacggttcc
    10501 cgaggaatgg cgttgcacga tcgccgcgcc gaacgcgtcg accatcacca ccacgtctcg
    10561 ttccgaactt gccggacggt caaacaccgc gagtttctgg gctccgtcgc cgctcaacac
    10621 gagccaactc gaggcgccat cggcgaaccg tcgggcatcg ctgcgctgca ggtcgcgcaa
    10681 tgtgcgcgag gtcacctcaa ggttggtggt cttgcgccaa cgagaccggt cgaccgtcgg
    10741 agcgatcacc gctccgtacg tcggcacgcg ccgctcatgg atcagcggat gcagtgcgta
    10801 gtcgatctcc tcggcaacga ggcggtcgag tgggttctcg acgtcgaggt cgacgccgtg
    10861 ctcgtcgagt tcgtcgacca gccgcctcag ccgcggcgat gggggatcgc tccagctcac
    10921 catgggctga cgatagcccc ttgcccacaa ttccctcgcc cgcatccctc catcgctggt
    10981 gttacccatg acacatggtg cagataccga ggccaaccct cttgacgcca cccgtactat
    11041 caattgtcga tccaaacgga tcgcgactcc ccgcgccgtt gcgacgcggc ggtcatccac
    11101 ggatctcaaa ggttgtgctc tcttggctaa gcagcgtccc gaacgcgacg aagaagacct
    11161 tgttcgcctc tacctgagcg acatcggcca atatccgctc ctcaccaagg acggggaggt
    11221 cgaactcgca aaggccatcg aagccggcac cgcggcgaag gcggagatgg aggcagccga
    11281 cggctcgctc accgcagccc gcaaacgcga acttcgccgc ctcatccgca ctggcgagga
    11341 agcggaactc gcgttcgttc agtcgaacct tcgcctggtc gtctccatcg caaagaaata
    11401 ccaggcatcc gggttgcccc tcctcgacct cattcaagag ggcaaccttg gcctgattca
    11461 cgccgtcgag aagttcgact ggcgcaaggg tttcaagttc tcgacctacg ccacgtggtg
    11521 gatccgccag gcgatcaccc gcggcatcgc gaacaccggt cgcacgattc gacttccagt
    11581 gcatgcaggc gacgtgctga gccgactcca aaaggcccga ttccggctcg aaaccaccct
    11641 cggtcgcccg gccaccctgc tggaactcgc cgaggaactc gacatgccgg tcgccaaggt
    11701 caccgaggcc atgcgctacg ccgccgacac cttgtcgttg tcggagccgc ttcgcgctga
    11761 cggcgacgcc gaactcggcg acatgatcga agaccgcggc gccgagtcgc cattcgatgc
    11821 cgcggccgtc gcgttgctgc ccgagcagat caaccgcctg ctcggcccac tcgacgaacg
    11881 cgaacgccag atcctcaagc tccgtttcgg gctcgatcgc ggcgaaccgc gcacccttga
    11941 ggaggtcggg gagcacttca acctgacccg tgaacgcatc cgccagatcg aagcgcgggc
    12001 catgtcgaag ctgcgccacc cgagctccga cactggtgcc aaggacctgc tcgcggtctg
    12061 atcgacctac gcgataccga acggcccacg tcttgcgacg tgggccgttt gggttttctg
    12121 cgctcgggat ccttcaggtt cgacctcaat caaccgaatg agaaggttca cgagtcgcgc
    12181 agggtgcgcg acctcggcca ggacgtagcg atgaactctt tcaccgacct tcgccaggat
    12241 gacgacgaca ccgacgaagc cgacgacttc cttcagcggc tgagcgaact cgatcagttc
    12301 gtcggagccc ctgacgccac gttctccccc gccatcgatc acttgggcga ttccgtcagc
    12361 tcaggcgatt cgggttccga cgaggccagc gattttctcg agcgcctcga cgacctcgac
    12421 cgattcgttt cggcgcaacg catcgacctg gatgcgtccg atgaggccga ggaattcctc
    12481 gagcgactcg gggaccttga ccgattcatg cccgccgacg cgaccgccga acactcacaa
    12541 gcggcgttct gtcctcgttg ccgacgtccc gcagcccccg gtgacccaga ggttgtgcga
    12601 gtcgttcaca tcctcggcac cgtcggaggt cgcccggtcg acggccagcc agcgttcatg
    12661 caccgctcgt gccgcgtcga caacgattcc gagttccgcg agattcacta atcgaacgcc
    12721 ccgattgagc cgcgttcgac cgccgcgtaa catccggcgg cgtccgccct cgtagctcag
    12781 ttggatagag cgaccgcctc ctaagcggta ggccacaggt tcgattcctg tcgagggcgc
    12841 cgattccacc cttgaccgtc cgtgaggcat ctacgatcga ctcacccgga gcaaggaccc
    12901 ttgatgaccc accacgacac gaccgctcgg ctccaccaga tcggcgccgt ggaggatcag
    12961 gtggggcttt cgcaacgcac gattcgccat tacgacgagg tcggcctggt caccccctct
    13021 gggcgttccc ccgggggatt ccggctgtac accgacgatg acgtcgcacg gttgaaacac
    13081 atcaaggcga tgaagccgct cggattcacc ctcgaagaat cgggagatct gctaaggatc
    13141 cgcgatcagc tcgaacgcgg cgaggcactc agcccagccg aactcgacta tctcacggcc
    13201 agcctgtcga gggccgagga gcggtgcgca aagctcgccc gccaactcga cgaagccaag
    13261 gccctcaccg caaccatgcg agccgagatc gactccgcca agcttcgccc ctgaccccgc
    13321 gagcgcgctc gtcgctcccg tcggacagtg ctgacctgag catgtcccac caggtcacag
    13381 cgagcgccgc gaccactcta cccttacgtt agggtagagt ggtcgccgat tctcgctccg
    13441 tgcctttggc cagcacctga acggaagacc cgcgatgcat gctgcgcaca acctgtcacg
    13501 agtgagcacc gcctggtgga ggaccgcacg gtacgcacga gcgatcggcc cacgcgttcg
    13561 gaaatacagc cggtattcac tcgccgagcg gcacgccttg gtgaccggcg gttcatccgg
    13621 aatcggggcg gagttgtgca aggaacttgt ctccaggggc gcatcggtca cgtcggtgga
    13681 cgtgaccccc ccaacgtgtg acgttgccgg catcacccac ctcgacggcg acgttcgaac
    13741 cttcgacttc ggttcgctcg ccccgatcga cctcatcgtg gcaaacgctg gcgtcgtcga
    13801 ttcgactcgc catcggcccc tcaccgagga cgccgtgcga cgcctcatcg acatcaacct
    13861 cgtcggcgtc gctcggacgc tgaccgctcc aaccacacca agtggtcgcg ccctgatcat
    13921 ctcgagtcgc tctgccgtag cgcccgaggc accgggtgcg ttgtacggcg ccacgaaggc
    13981 cgcagcgctc aactacgggc tctccgcagg atcggtgcgc ccgacgaccg tcgcgttggt
    14041 cggcatcacc gccaccaacc tgttcgctgc ggaggccgac taccaaggcc gcgctgtcga
    14101 cccaccccga tcgacatttg gcgatccgtc cgaggtcgct cgtgcgtgtc tcgacgcggt
    14161 cgaggccggc gacccgctcg tcatcaccga ccgcgacacg ctccgggacg tctcagcgtt
    14221 cgggttcgcg ttcctcacgg cacgactccg ttgaccggcg tcacacatcc gcagcgatat
    14281 cggcttcact acgggacgag cacgcggccc tttttcgctc tgggacgaca ttcggtagtg
    14341 ttcaccgcac aaccggccgc gcagcagatc gtcgcgagcg atcaaccgac aacggcgtcg
    14401 gctgatcgac gaggcagcgg gggcgatcaa accgtgtcct cgacgcaatc agggcaacga
    14461 caacactgca ccgagaccca tctcgccgcc aacggagccg gcgagctggg caggtggctg
    14521 acttgcggtg cccgtcggtt cgggcaaccg cgaccaacga gagggacccc gccgtgaccg
    14581 atcagtacct tgccgacgca ttcaacctgg tggcgcaccg aaaccctggg gaaagcgagt
    14641 tccaccaggc ggtcaaagag gtgctcgaca cccttgaggt cgtgctgaaa aagcacccgg
    14701 aaatggccga acaacgcctg atcgaacgcg tctgtgagcc ggaacgccag atcatcttcc
    14761 gggttccgtg ggtcgacgac gaggggtgta tccagatcaa tcggggcttt cgcgtcgagt
    14821 tcaacagcgc cctcggtccc ttcaagggcg gcctgcgatt ccacccctcg gtcaacgtgg
    14881 gcatcatcaa gttcctcggt ttcgaacaga cgttcaagaa cgccttgacg ggcatgccga
    14941 tcggtggcgg caaaggcggc tccgatttcg accccaaggg aaaatccgac ggtgagatca
    15001 tgcggtttt
//
LOCUS       DAOOMA010000004        14921 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_4270_length_14921_cov_4.166824, whole genome shotgun sequence.
ACCESSION   DAOOMA010000004 DAOOMA010000000
VERSION     DAOOMA010000004.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 14921)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14921)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14921
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4270_length_14921_cov_4.166824"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2120)
                     /locus_tag="PLP95_00240"
     CDS             complement(<1..2120)
                     /locus_tag="PLP95_00240"
                     /inference="COORDINATES: protein motif:HMM:NF024867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPB44263.1"
                     /translation="MSLFEVEPGTDHDEPAPAVGAGTRLHRLEILNWGTFHEKVWTLD
                     LGGRNALLTGDIGSGKSTMVDAVTTLLVPAQRISYNKAAGAETRERDLRSYVLGFYKS
                     ERNEEAGGSRPVGLRGPEHFSVILGVFRDIERATTTTLAQVFRAREDTSQPERFFVVF
                     DGELSITEHFSNPGDSLADLRKQLKTLGANVSDGFNEYRRSFRRRLGITSDQALDLFL
                     QTVSMKAVDNLNEFVRSHMLEPVDMSKRIDALVAHFDDLTKAHDAVVRARDQLELLVP
                     LVGHLDAYDEHSEAAQRLGRLIETAPFYFATAHDWLLEIKAERLAGELDRLQRLDADI
                     GTRLDQLRTDERHLHAQIDGNGGNRLALIESELRRLRGELPRRQHAVDEHNRKLEAVG
                     LEPVRDEAQFIERRSQLGQRERSVEAERVDVDNKIGELTVRDRDERVAIDEITNELDS
                     LAKRRSNIPSRSLELRERLIGDLRLTPDELVFAGELMAIDDQYDRWEGAAERVLHSFA
                     LSLLVPNERYREVAAWIDSHHLRGRIVYYRVPERVAPAGDPNRHAAYPLLMDMVNIRP
                     DTPFAEFIRRELTHRADYLCVPDTSTFSTLDRAITPAGQIKSKDRHEKDDRSRIDDRS
                     RYVLGWDNRAKVEALRAERETLIGNHAESRRLLDVAQADQRRLISMGTTLIDLARVDD
                     WVSLDVESLTKQVEDLSGEHETLRA"
     gene            complement(2117..2764)
                     /locus_tag="PLP95_00245"
     CDS             complement(2117..2764)
                     /locus_tag="PLP95_00245"
                     /inference="COORDINATES: protein motif:HMM:NF025205.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4194 domain-containing protein"
                     /protein_id="HPB44264.1"
                     /translation="MTTRSPGESSRSGQATHSPHLAPDDLSLVAIALMRGPITREHDQ
                     KLWPALIKIEHRVADYVGVLGLRLVLDESDGYAFLRSAREDEVEGEFPRLVNRHRLPF
                     QVSLLIALLRKRLAEFDATSSDARLMLTRDQITDLMRTYIPDSSDDVKLRRKVDEHIA
                     SVEKLGFLKKLRSDYDAYEVRRIIRAFVDGQWLADFDRRLAEYVEQAGLADQEAP"
     gene            complement(2761..4260)
                     /locus_tag="PLP95_00250"
     CDS             complement(2761..4260)
                     /locus_tag="PLP95_00250"
                     /inference="COORDINATES: protein motif:HMM:NF023283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3375 domain-containing protein"
                     /protein_id="HPB44265.1"
                     /translation="MDFDEIEHLKARHGAWSLLRADNVALVLSFLSRVFLDANQANLP
                     ASKVLSELDDELYALNQRIGESRFPKSAKAYLDDWSASERGWLRRHVPAGADEPHYDL
                     TPSVERAVAWVEGLRSRSFVGTESRLGIVVELLRQLIYGAAEDSSERLADLRRRRDEI
                     DATIAQLEAGELGVDDPVVQRDRYQQFSRTARELLGDFREVEENFRELDRDLRAKIAG
                     WTGSKGELLDDVVSTRSGIADSDQGRSFAAFYDLLLSGERQQELADLLARLRDLDDIP
                     EFDPRLERIQRDWLDASERTQATIRQLSEQLRRFLDDQVWLENRRVFDLLRSIEANAL
                     AVRDVAVVDVHTEVDDTRVSLRLPMERPLYQKVRTTSLQNSTIEIGTGDVDSKLLADH
                     PFIDTVLLTQRVLASLGAGEQVGLNQVIDETPLDEGLAEVIAYLTLADESLDVDISEQ
                     DRTELSWTESTWPHGVAPDADDVEDVDEVVLRVLDLPAVTFKRTPGGPS"
     gene            4421..5389
                     /locus_tag="PLP95_00255"
     CDS             4421..5389
                     /locus_tag="PLP95_00255"
                     /inference="COORDINATES: protein motif:HMM:NF013705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ C-terminal domain-containing protein"
                     /protein_id="HPB44266.1"
                     /translation="MASSYYDTLGVSRNATSDEIQKAYRKLARVWHPDVNQSSEAEDR
                     FKAISEAYDVLSDAETRKRYDAFGADFRKVPPGTDPAAWARAQRDATTGNGGGFGGGG
                     FSGGFGSAFGGGGGGGGGGGGGGGFEDLLGGLFGQGRRSSGPQRGADQDAEIILSLEE
                     AFGGGERQVTMSGPTGVRNITVKIPVGVTAGQKIRVRGEGGPGSGGGPAGDLLLKVKL
                     APDPTFTVDGRDLSVTLAVSPWEAALGAKATVPTLSGPAQVKVPPGSSSGKRLRLRGR
                     GLPNRRGDDGDLYAVISIVVPESLTDREADLFEQLAQESLFKPRGV"
     gene            complement(5411..5950)
                     /locus_tag="PLP95_00260"
     CDS             complement(5411..5950)
                     /locus_tag="PLP95_00260"
                     /inference="COORDINATES: protein motif:HMM:NF018590.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1269 domain-containing protein"
                     /protein_id="HPB44267.1"
                     /translation="MPDTAQPQLDQTLVGLSFEDGFRATEFLTATARLAASGYLRIID
                     AVIVTKDEQGKTTVRETTDLQIGESALSGALWAGLFGVVLGGPVGWLAGMAVGAGAGA
                     LTAKVVDLGLSDEWVDWFREVVQPNTTTVALLATDIDNNALVAEAERFSGARLVYTNL
                     PPATLDRVKQALNDNSASD"
     gene            6034..7419
                     /locus_tag="PLP95_00265"
     CDS             6034..7419
                     /locus_tag="PLP95_00265"
                     /inference="COORDINATES: protein motif:HMM:NF024850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HPB44268.1"
                     /translation="MVVRTTFRRRIRRTVALLGAAAVIGGCTNASFDDTIGDAPAPTF
                     VTPSTNDVATVDDSPFATMTGIARSWSSNPDFSGRLAGIDPTATDQAAAASAYDAVII
                     AALAAEEARSDAPGRIASAIPGITVGGEPCLGFANCRAKILKRTDIAYTGYSGSAALE
                     RNGIPLEGEFEVVRISTNGVPTASESVGGQLRSPGELGESSGADPRSGPLPDGILRVG
                     TLLPTDLDSPGLTTASRSAMAGVRLAVEEINEYTVGGVIGSDLDLLDDASGTGTPDDI
                     ARGAQALVDAGADVIIGGLDSTTTQRVLNVVRPAGVVLISPLDGGAAQTAGADGRFFR
                     TTPTAELQGWVVGQLASFDGHTGVVIVDGASDDERVVADNMAMAFTSLDGTITSRVEL
                     TSADQAAAVAAIVAAAQPTAILITADVDSTAAFARAMHADGFGPTEIGWYGAPWLVTD
                     DAAAVFNSTGG"
     gene            complement(7420..8157)
                     /gene="pdxH"
                     /locus_tag="PLP95_00270"
     CDS             complement(7420..8157)
                     /gene="pdxH"
                     /locus_tag="PLP95_00270"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: protein motif:HMM:NF004231.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine-phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HPB44269.1"
                     /translation="MSGVDPASVVGADDAAGSDGSATGSFVGERSTYEHGVLIESEVA
                     DNPIDQLRRWIDEAEQAAVPEPTAMTLSTVSSTGRLTSRTVLLRQLSAEGPVFYTNYS
                     SAKADDLEAHPNCAAQLLWIPLQRQVRIEGTARPVPPATSDAYFADRPRGSQVGAWAS
                     PQSEVIAGRAELEAMVADAEARFEHVENVPRPPHWGGYELVADVVEFWQGRPSRLHDR
                     LRYRRVGPEGERRSDGERDWVLERLAP"
     gene            complement(8154..9449)
                     /gene="serS"
                     /locus_tag="PLP95_00275"
     CDS             complement(8154..9449)
                     /gene="serS"
                     /locus_tag="PLP95_00275"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015797664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HPB44270.1"
                     /translation="MLDIRLFRNDLETVSEGLARRGEDPEAIAAVVSLDAEVRRVNAE
                     RDELRSRIRALSSEVGQLMRDGQRELAAELQAESRSLGEHERELDQRSDVASAELHEL
                     LLRIPNLPSDDAPAGRSEHDNVVARIEQFEESAYGEHQRVAHWDIGEALGILDVERGV
                     KMSGSMFVMYRKAGAQLVRALCQLALDRNADLYEEVRPPTLVRTDTMVATGHLPKFAD
                     DAYHLERDDLYAIPTAEVPLTSLARGEIIAEAELPMRLMAHTSCFRREAGSAGKDTRG
                     LLRVHEFDKVEILALGTAAQAAELHAEILQRAESLIRDLGLAYRVLDLCAGDLGNSSR
                     RTFDLEVYAPGVDRWLEVSSVSWFGDYQARRANIRYRLAEGKGTEVCHTLNGSALAVP
                     RVWAGIVETYRQPDGTVVIPEILRPYMRGLERIELPVRP"
     gene            complement(9491..10171)
                     /locus_tag="PLP95_00280"
     CDS             complement(9491..10171)
                     /locus_tag="PLP95_00280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB44271.1"
                     /translation="MEVRSWMVPLVAAALFAAVAGGCSSGDGTSAGDSTARSSDDVST
                     SATVAISEKSPVQAALAKLTSVSTNDLRMGAAFNHDLVSIGMRSMGFDVSESACIADA
                     VAASMGEAFDRTPVASLFGSDSMSPQVLLGCTTLERAQELASGADAAQVPEAEMRQVL
                     VGLASAGYEQAGLTPAEATCLAADAVATLEGDGSFAALQSGGLSDGINEVALRRCVSD
                     DRIVELAK"
     gene            10243..10896
                     /locus_tag="PLP95_00285"
     CDS             10243..10896
                     /locus_tag="PLP95_00285"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HPB44272.1"
                     /translation="MNHDPANDAVIFDVDGTLWDSAPGILSCLVETFEYFDLEVPGEV
                     ALRAALGPPLDMMLRDLGFPSGQIEAGRLEYRRRYFEHGEAECEVYAGIVELLERLAA
                     NGRSLAVATSKGRETAHRMLEAFGLTEYFDSIRAADMATAAHGKVHLIAAALTDLNTR
                     SAVMVGDRNFDIEGGLSNGLYTIGVNWGYAPTGELEAAGAHVIVDTVSQLASVLIDR"
     gene            10907..11884
                     /locus_tag="PLP95_00290"
     CDS             10907..11884
                     /locus_tag="PLP95_00290"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPB44273.1"
                     /translation="MSAIVVDHLSVRYGDLVAVHDIGFVAEPARITVLLGPNGAGKTS
                     TIECIEGYRRPSGGTIRIGNIDPFTERREFARRVGVMLQHGGIQPAVRPMEILRLYEA
                     LYDNAHSPADMLAFVGLQNRAKSPWRSLSGGEQQRLALALAVIGRPQVVLLDEPTAGV
                     DIAGRKLIRDLIADLAAQGVTVLLTTHDLAEIEVLADDIVIIDHGRVVAAGSASDLLS
                     GADAPSFRFRAQPGLDLAALSAVLGVAVTDLGEGMYDVEASPTPPLVASLTGWLAERS
                     ILLGDLQAGRQGLEQLFVRLTSETAGADRRSESGRRTRAQRRSPRTGQR"
     gene            11881..12765
                     /locus_tag="PLP95_00295"
     CDS             11881..12765
                     /locus_tag="PLP95_00295"
                     /inference="COORDINATES: protein motif:HMM:NF013245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPB44274.1"
                     /translation="MRAFTAQLRLELILAFRNGEQILVNLLIPLGLLVLMANIDIFDE
                     STEPIQRLGPTILALAVMSSSLVSLGVGTGFERFYGVLKRLGATPLGRGRWVAAKFGS
                     IMCIELVQWCVIVVVALALGWSPQASGWLPAIAAAALATSAFGGLALLLAGTLKGLTN
                     LALCNALYLVLLVTGGMVIPLAEMPTPMATAAKVFPAGTVTDHARGIPSGGIDPFERV
                     ARPDRLGRRDPAVGDSILPLGVIVTRTPARPCDAQSGTFAETTKPAAHRAGHARCATG
                     LSRSVLAPVGRRACPDGQ"
     gene            complement(12759..13187)
                     /locus_tag="PLP95_00300"
     CDS             complement(12759..13187)
                     /locus_tag="PLP95_00300"
                     /inference="COORDINATES: protein motif:HMM:NF015582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome C oxidase subunit IV family protein"
                     /protein_id="HPB44275.1"
                     /translation="MSAVGATHAHHDDHADHGDGLYWRTFVALAILTAVEVSTEWWPE
                     GLGWITTAALIVMMTIKFILVAQIFMHLRDDHNLLGQLFYFGAILAIAVFMGVFGSMR
                     IFEKSGLGGEFTFNDPTMQRPLPPPPTDPPPIIRETTGGH"
     gene            complement(13198..13848)
                     /locus_tag="PLP95_00305"
     CDS             complement(13198..13848)
                     /locus_tag="PLP95_00305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-copper oxidase subunit III"
                     /protein_id="HPB44276.1"
                     /translation="MSEALTMPVAAVDDHGHPPTNTGISNEKLGMWVFLGSECLMFGG
                     LISTYFLYKNRPGSIVSVRGREITGSDIRPSDLFANGFTSVTSFILLASSLTMVFAVA
                     AAKRNEMARMRYWLGTTAVLGSLFIGGQVYEFTTFVREGMGYTSNVAASAFYTLTGFH
                     GAHVTIGILMLLITIAMSARGRIPHAKAEAVEIVGLYWHFVDVVWILVFTIVYLIK"
     gene            complement(13841..>14921)
                     /locus_tag="PLP95_00310"
     CDS             complement(13841..>14921)
                     /locus_tag="PLP95_00310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020379246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 4"
                     /protein_id="HPB44277.1"
                     /translation="PISMAAFSLSTMFIAVPTGVKILNWVATMYGGRLRFTAAMMFAM
                     GLVSMFTIGGLSGVTHAFAPADTQQTDTYYIVAHFHYVLFGGALLGFFGAMYFWWPKI
                     FGHFLDERMGKAHFWMTLVGFNLTFGPMHILGLQGMSRRYFTYSDGYGFNFWNLVATI
                     GAFMIAIGLVQFLVNCWVSYRKWKREPYDPGADPWDSRSLEWITPSPTPEYNFSETPV
                     VHELDDFWHRKYGHDEQGRMVRIAETDDIVQKRVSGDDIHLPSPSYWPIVLSAGLPFI
                     AYGLIFSYWWLILGAGLTVAGIAGWIVEPSTDPDAGHDHHDAHDDGDDDHGEAITATD
                     VAGELETVGAGVAGGAAASEENAGE"
BASE COUNT         2637 a         4987 c         4768 g         2529 t
ORIGIN      
        1 gcccgcagcg tttcgtgttc gcccgacagg tcctccacct gcttggtgag ggactcgacg
       61 tcgaggctga cccaatcgtc cacacgggcg agatcgatca acgtggtgcc catcgagatc
      121 aggcgtcgtt ggtcggcctg cgcgacgtcg agcaggcgcc gcgactcggc gtggttgccg
      181 atgagtgtct cgcgctcagc ccgcagggcc tcgacctttg cccggttgtc ccagccgagt
      241 acgtatcgcg accggtcgtc gatacggctg cggtcgtcct tttcgtgacg gtccttcgat
      301 ttgatctgac ccgccggggt gatcgctcga tcgagggttg aaaacgtcga ggtgtcgggg
      361 acgcagaggt agtcggctcg atgggtgagc tcgcgcctga tgaactccgc gaacggggta
      421 tcgggccgga tgttcaccat gtccatgagc aagggatacg cagcgtgacg attcgggtct
      481 ccggccggcg cgacccgctc gggcacccgg tagtagacga tgcggcctcg aaggtggtgc
      541 gaatcgatcc acgccgcaac ctcgcgatac cgctcgttgg gaacgagcag cgacagtgcg
      601 aagctgtgaa gcacccgctc cgccgcgccc tcccagcggt cgtattggtc gtcgatcgcc
      661 atcaattccc cggcgaacac caactcgtca ggggtgagtc gaaggtcgcc gatcagccgc
      721 tcccgaagct cgaggctgcg cgacgggata ttcgaccgcc gtttcgcgag gctgtcgagt
      781 tcgttggtga tttcgtcgat cgcgacgcgc tcgtcccggt cgcgaaccgt caattccccg
      841 atcttgttgt cgacatccac acgctcggcc tcgaccgagc gttcgcgttg gccgagctgg
      901 cttcggcgct cgatgaactg cgcttcgtcg cgaaccggtt cgagcccaac cgcttccagc
      961 ttgcggttgt gttcgtccac ggcgtgttgg cgtcggggaa gctcgcctcg caggcgtcgc
     1021 agttcgctct cgatgagggc cagccggttg ccgccgtttc cgtcgatctg agcgtgcaga
     1081 tgacgttcgt cggtgcgaag ttgatcgagg cgggtcccga tgtctgcgtc gagacgttgc
     1141 agccggtcga gttcacccgc aagccgctcc gccttgattt ccaacagcca gtcgtgtgcc
     1201 gtcgcgaagt agaacggtgc cgtctcgatg aggcgaccca accgctgcgc cgcttcgctg
     1261 tgttcgtcgt aggcgtcgag gtgcccgacg agcggcacga gcaattcgag ttggtctcgg
     1321 gcgcgaacga ccgcgtcgtg ggccttggtg aggtcatcga agtgagcgac gagcgcgtcg
     1381 atccgcttcg acatgtcgac gggttccaac atgtgggacc gcacgaactc gttcaggttg
     1441 tcgaccgcct tcatcgacac ggtctgcagg aacaggtcga gcgcctgatc ggaggtgatg
     1501 ccaagtcgtc gccggaaact gcgtcggtat tcgttgaacc cgtcgctgac gttcgcgccg
     1561 agcgtcttga gttgcttgcg aagatcggca agcgagtcgc ccgggttcga gaaatgctcg
     1621 gtaatcgaaa gttcgccgtc gaagacaacg aagaatcgtt cgggctggga ggtgtcctcg
     1681 cgtgcccgaa acacctgcgc gagggtggtt gtggtggcgc gctcgatgtc tcgaaacacc
     1741 cccaggatca ccgagaaatg ctcggggccg cgcaacccga ccgggcggga accaccggcc
     1801 tcctcgttgc gttcggactt gtagaacccc aacacatagg agcggaggtc tcgctctcgg
     1861 gtctccgccc ccgcagcctt gttgtaggag atgcgctggg cgggcaccaa caacgtggtg
     1921 accgcatcga ccatcgtgga cttccccgac ccgatgtcac cggtcaggag cgcattgcgc
     1981 ccacccaggt cgagggtcca caccttctcg tggaaggtgc cccagttcaa gatctccagt
     2041 cggtgcagcc gcgttccggc acccaccgca ggggccggct catcatggtc ggtgcccggc
     2101 tcgacttcga acaggctcat ggcgcctcct gatcggccag gccggcctgt tcgacgtact
     2161 ccgccaagcg acgatcgaag tccgcaagcc actgcccgtc gacgaacgca cggatgatgc
     2221 gacgcacctc gtacgcgtcg tagtcgctgc ggagcttctt gaggaatccc agcttctcga
     2281 ccgacgcgat gtgctcatcg accttgcggc gcaacttgac gtcgtcggag ctgtcaggga
     2341 tgtaggtacg catcaggtcg gtgatctggt cgcgggtcaa catcagccgg gcgtccgaac
     2401 tcgtggcgtc gaactcagcg agtcgcttgc gaagcaacgc gatcagaaga ctcacctgaa
     2461 agggcaggcg atgccggttg acgagtcgag gaaattcgcc ctcgacttcg tcttctcggg
     2521 cgctgcggag gaacgcataa ccgtcggatt cgtcgagcac gagccgcagt ccgagcaccc
     2581 cgacatagtc ggcgacccgg tgttcgatct tgatcagggc cggccacagc ttctggtcgt
     2641 gctcgcgggt gatcgggcct cgcatcagcg cgatggcgac gagcgagaga tcgtcggggg
     2701 cgaggtgcgg gctgtgggtg gcctggccgc ttcggctcga ttcgcctggg cttcgggtcg
     2761 tcacgacggg cctcccgggg ttcgtttgaa ggtgacagcg ggaagatcga ggacgcgcag
     2821 gactacctcg tcgacatcct cgacgtcgtc ggcgtcgggc gcgaccccat ggggccacgt
     2881 ggactcggtc cagctgagct cggtgcgatc ctgttcggag atgtcgacgt caagcgactc
     2941 gtcggcgagg gtcaggtacg cgatgacctc ggcgagtccc tcgtcgagcg gcgtttcgtc
     3001 gatcacctgg ttcaaaccaa cctgctcgcc cgcgccgagg ctggcgagca cccgctgggt
     3061 gagcaacacg gtgtcgatga acggatgatc tgcgagcagc ttggaatcga catcgccagt
     3121 gccgatttcg atcgtcgagt tctgcagcga cgtggttcgc accttctggt agaggggacg
     3181 ctccatcggg aggcgcagcg agaccctggt gtcgtcgacc tcggtgtgga catcgaccac
     3241 agcaacatct cgcaccgcga gagcgttggc ttcgatcgaa cggagcaggt cgaacacccg
     3301 gcggttctcc aaccagacct ggtcgtcgag aaaccgtcgg agttgctcgg aaagctggcg
     3361 gatcgtggcc tgagttcgct cgctcgcatc gagccaatca cgctgaatcc gttccagcct
     3421 ggggtcgaac tcgggaatgt cgtcgaggtc gcgaagccga gccaacagat cggccaactc
     3481 ctgttgacgt tcccccgaca gcaagaggtc gtagaacgcc gcgaaactcc ggccttgatc
     3541 tgagtcggcg ataccgctgc gcgtcgacac gacatcgtcg agcaattcgc ctttcgaccc
     3601 ggtccatccg gcaatcttgg cccgaaggtc tcggtcgagt tcccggaagt tctcctcgac
     3661 ctcgcgaaaa tcgccgagca gttcgcgggc cgtgcgggag aactgctgat aacggtcacg
     3721 ctgaaccacc gggtcgtcca cgccgagttc tcccgcctcg agctgggcga tcgtggcatc
     3781 gatctcatcg cggcgccgtc gcagatccgc gagacgttca gacgagtcct ccgcggctcc
     3841 atagatcagc tgacgcaaga gctcgacgac gatgccaagg cgcgactcgg ttccgacgaa
     3901 gctgcgagat cggaggcctt cgacccacgc gaccgccctt tccaccgacg gggtgaggtc
     3961 atagtggggt tcgtcggcac cggcgggaac gtggcggcgc aaccaacccc gttcggaggc
     4021 ggaccaatcg tcgaggtagg ccttggccga tttcggaaac cggctttcac caatccgctg
     4081 gttgagcgca tagagctcgt cgtcgagttc gctcagcacc ttcgatgccg ggaggttggc
     4141 ctggttggcg tcgagaaaga cccggctcaa gaaactgagc accagcgcga cgttgtcggc
     4201 gcgcagcagc gaccacgcgc catgtctggc cttcaggtgt tcgatctcgt cgaaatccac
     4261 gcgcacatgc tcccattcga cagccgcgac cgccgattcc aagttccatg atggccgaac
     4321 ttcgccgaat cggcggccgt cgcgtcgagc gagtggccgc cggacgatcc gcacgaccct
     4381 ccgcacccag cgacgggcgt ggaccgctac cgtgaacgcc atggcgagca gctattacga
     4441 cacgctcggg gtctcccgca acgccacctc ggacgagatc cagaaggcgt atcgcaagct
     4501 cgcccgcgtg tggcatcccg acgtcaacca gtcgtccgag gccgaggacc gcttcaaggc
     4561 gatctcggag gcctacgacg tcctgtccga cgccgagacc cgcaaacgct acgacgcgtt
     4621 cggtgccgac ttccgcaagg tgccgcccgg aaccgacccg gcggcgtggg cccgagcgca
     4681 acgggacgct accactggca acggaggcgg attcggcggc ggcgggttca gtggaggatt
     4741 cgggagtgcg ttcggaggcg gaggtggcgg aggaggcgga ggtggcggag gaggcggctt
     4801 cgaggacctg ctcggcgggt tgttcggtca gggccgtcgc agcagcggcc cgcaacgcgg
     4861 agccgaccag gacgcagaga tcatcctgtc gctcgaggag gccttcggcg ggggtgagcg
     4921 tcaggtcacg atgagcggcc cgacaggcgt ccgcaacatc accgtgaaga tccccgtggg
     4981 agtgaccgcg ggccaaaaga tccgggtccg cggcgagggg ggaccgggat ccggcggcgg
     5041 acccgccggc gacctgttgt tgaaggtgaa gctggcaccg gatccgacct tcaccgtcga
     5101 cggacgggac ttgtcggtca cactcgcggt gagcccgtgg gaggcggcgc tcggcgccaa
     5161 ggccacggtg cccacgttga gcggccccgc ccaggtcaag gtgcccccgg ggtcgtcgtc
     5221 gggcaagcga ctgcggttac gcggccgcgg gctgcccaac cggcgcggcg acgacggcga
     5281 cctgtatgcc gtcatctcca tcgtcgttcc cgagtcactc accgaccgcg aggccgacct
     5341 gttcgaacag ctcgctcaag agtcgctgtt caagccccgc ggcgtgtaac cccccggagg
     5401 acgacgccgg tcagtcgctc gcgctgttgt cgttgagggc ctgtttcacc cggtcgaggg
     5461 ttgccggcgg gaggttggtg tagacgagcc gcgctccgct gaatcgttcg gcttcagcaa
     5521 cgagggcgtt gttgtcgata tcggtggcga gtaaagcaac cgtcgtggtg ttgggttgga
     5581 ccacctcccg gaaccaatcc acccactcat ccgacaggcc caggtcgacc accttggccg
     5641 tcaaagcgcc tgcccccgct ccgaccgcca tgccggcgag ccatccgacc ggaccgccaa
     5701 ggaccacgcc gaacagtccg gcccacagtg ctcccgacaa cgccgactcg ccgatctgaa
     5761 gatcggtcgt ctcacgcacg gtcgtcttgc cctgctcgtc cttggtcacg atcaccgcgt
     5821 cgatgatccg cagatagcca ctcgccgcca accgcgccgt cgccgtcagg aactcggtcg
     5881 cccgaaaccc gtcctcaaag gaaagtccga cgagggtctg gtcgagctgt ggctgcgcgg
     5941 tatcaggcat ctcgccagta ttgccggtcg gctgccgcca gccaaagaca tcgcgagccg
     6001 tgacaccacc cgggcacctc taacatgaca gagatggttg tgcgcaccac gtttcgacgc
     6061 cgcatccgcc gaaccgtggc gctcctcggc gcggcggccg tgatcggcgg ttgcacgaac
     6121 gcgagctttg acgacacgat cggcgatgct ccagcgccaa catttgtgac cccgtcgacg
     6181 aacgacgtgg cgacggtcga cgacagtccg ttcgccacga tgaccggcat cgcccgctcg
     6241 tggagctcaa atcccgactt cagcggacgc ctggccggaa tcgatccgac cgccaccgat
     6301 caagcggctg cagcctctgc ctacgacgcc gtgatcatcg ctgcattggc cgccgaagag
     6361 gcgcgcagtg acgccccggg ccgtatcgcc agcgccatcc ccggtatcac cgtcggcggc
     6421 gagccgtgtc tcgggttcgc gaactgtcgc gcgaagatcc tcaaacgaac cgacatcgcg
     6481 tacacgggct actccggctc ggccgcgctc gaacgcaacg gcatcccgtt ggagggcgag
     6541 ttcgaggtcg tgcgcatctc caccaacggc gtgccaaccg cttcagagtc cgtcggtggg
     6601 cagctgcgca gccccggaga actcggcgag tcctcgggtg ccgacccccg ctcagggcca
     6661 ttgcccgacg gcatccttcg cgtcgggacc ctgctgccga ccgacctcga cagccccgga
     6721 ctcaccacgg cctcgcgctc cgctatggcc ggggtgcgtc tcgcggttga ggagatcaac
     6781 gagtacacgg tgggtggagt catcggttca gacctcgacc tcctcgacga tgcctccggc
     6841 accggcaccc ccgacgacat cgcccgagga gcgcaggccc tcgtcgatgc tggcgccgat
     6901 gtcatcatcg gcggcctcga ttcgacgacc acgcaacgag tgctcaacgt ggtccgaccg
     6961 gcgggcgtcg tcttgatctc gccgctggat ggtggcgcag cccaaacggc cggcgccgac
     7021 ggaaggttct tccgcaccac gccgaccgcc gagttgcagg gctgggtcgt cggacaactt
     7081 gcgtcgttcg acggacacac gggcgtcgtc atcgtcgacg gtgcgagcga cgacgaacga
     7141 gtcgtcgccg acaacatggc gatggcgttc accagtttgg acgggaccat cacgagtcgc
     7201 gtcgagctca catcggccga tcaggccgcc gcggtggcgg cgatcgtcgc agcggcccag
     7261 ccgaccgcga tcctgatcac cgctgatgtc gactcgaccg cggcgttcgc tcgcgccatg
     7321 catgccgacg ggttcggccc gaccgagatc ggctggtacg gcgcaccatg gctcgtcacc
     7381 gacgacgccg cggcggtatt caactcgacc ggcggctgat cacggcgcaa gccgttcgag
     7441 cacccagtcg cgctcgccat cagaccggcg ctcgccctca ggcccgaccc tgcggtagcg
     7501 cagtcggtcg tgaaggcgac tcggccggcc ctgccagaac tccaccacat cggccacgag
     7561 ttcgtaccca ccccaatgcg gtggtctcgg gacgttctcg acatgttcga atctcgcctc
     7621 ggcgtcggcg accatcgcct ccagttcggc acgcccggcg atgacctccg actgcggcga
     7681 cgcccacgca cccacctggc tgccgcgagg acggtcggcg aagtacgcgt ccgacgtcgc
     7741 tgggggaacc ggccgagccg tgccctcgat ccgcacctgt cgttgaagcg ggatccacag
     7801 caactgcgcc gcacagttcg gatgcgcctc aaggtcgtcg gccttcgcac tgctgtagtt
     7861 cgtgtagaac accggaccct cggcgctcag ctgtcggagc agcacggtgc gcgaggtcag
     7921 cctgccggtg ctcgacaccg tcgacagggt catcgccgtc ggttcgggga ccgccgcctg
     7981 ttcggcttcg tcgatccagc gacgaagctg gtcgatcggg ttgtcggcca cctcggattc
     8041 gatcaggacg ccgtgttcgt aggtcgaccg ctcgccgacg aacgatcccg tcgccgatcc
     8101 gtctgagcct gcggcatcgt cggcgcccac gacgctcgca ggatcgacac cactcatggg
     8161 cgtaccggca actcgatgcg ttcaagacct cgcatgtacg ggcgcagaat ctccgggatg
     8221 accacggtgc cgtcgggctg tcgataggtc tcgacgatgc ccgcccacac ccgtggcacg
     8281 gcaagcgcgg agccattcaa ggtgtgacac acctcggttc ccttgccctc ggccagccgg
     8341 tagcggatgt tggcacgacg ggcctggtag tcgccgaacc acgacaccga ggacacctcc
     8401 aaccaacggt cgacacccgg ggcgtacacc tcaagatcga acgtacgacg agaggaattg
     8461 ccgaggtcgc ccgcgcacag gtccagcacc cggtaggcga ggccgagatc tcggatgagc
     8521 gactcggcgc gttggaggat ctctgcgtgg agttccgccg cctgcgccgc ggttccgagc
     8581 gccagaatct cgaccttgtc gaactcgtgc acccgcagga gcccacgggt gtccttgcca
     8641 gcggacccgg cttcgcgccg gaagcacgac gtgtgcgcca tcagccgcat cggcaattcg
     8701 gcctcggcga tgatttcccc acgggccaag gaggtcagcg gcacctcggc ggtcgggatg
     8761 gcgtacagat cgtcgcgctc gaggtgatag gcgtcgtcgg cgaacttcgg aaggtggccg
     8821 gtcgccacca tcgtgtcggt acgcaccagc gtcggcggcc gcacctcctc gtagaggtcg
     8881 gcgttgcggt cgagcgccag ctgacagagc gcacgaacga gttgggcacc agccttgcgg
     8941 tacatgacga acatcgagcc cgacatcttc acgccccgct cgacatcgag gatcccgagt
     9001 gcctcaccga tgtcccagtg cgccacccgc tggtgctcgc cgtaggccga ctcctcgaac
     9061 tgctcgatgc gcgccaccac gttgtcgtgc tcggatcgac ccgccggggc atcgtcggac
     9121 ggcaggttcg gaatgcgcaa caacaactcg tgcaactccg cagaggcgac atcggatcgc
     9181 tggtcgagtt ccctttcgtg ctcaccgagg gagcgggact cggcctgcaa ctccgcagcg
     9241 agttcacgct gtccgtcgcg catcagttga ccgacctcgc tcgacaacgc gcgaatacgc
     9301 gaacgcaatt cgtcacgctc ggcgttcacc cgccgaacct cggcgtcgag cgacacgacg
     9361 gcggcgatcg cctcgggatc ctcaccgcga cgcgccaagc cttcgctcac ggtttcgagg
     9421 tcgttgcgga acagtctgat gtccaacatt cctgcaacgc tatcgccgac cggacccaac
     9481 cgaatcccgg ttacttcgcg agttcgacga tccggtcgtc gctcacacac ctccgcagcg
     9541 ctacctcgtt gatcccgtcg gaaaggccgc ccgactgaag cgccgcgaac gacccgtcgc
     9601 cttcgagcgt cgcgaccgca tcggccgcca gacaggttgc ctcggcgggg gtcaaccccg
     9661 cctgctcgta ccccgccgac gcgaggccca cgagcacctg gcgcatctcc gcttctggca
     9721 cctgcgcagc gtctgcgcca gaggccagct cctgggcacg ttccaacgtc gtgcaaccca
     9781 ggagcacttg tgggctcatc gagtcggaac caaaaagcga ggcgaccggc gtacggtcga
     9841 acgcctcccc catcgacgcg gcgacggcgt ccgcaatgca ggcggactcg ctgacgtcga
     9901 aacccatcga acgcatcccg atcgagacca agtcgtgatt gaacgcagcg cccatccgca
     9961 gatcgttggt cgacactgag gtgagcttcg caagcgccgc ctgcaccggt gacttctcgg
    10021 agattgcgac ggtggctgaa gtcgacacgt cgtcactcga ccgcgccgtc gaatctccag
    10081 ccgaggtgcc atcgccgcta ctgcatcctc cggcgacggc cgcgaacagc gcggccgcca
    10141 ccaagggaac catccaggaa cgaacctcca ccaccggtac cctacgccag gcgcggtcgc
    10201 ccatcggcgc cacggtccct ccagcggtta gcttctggac acgtgaacca cgacccagcg
    10261 aacgatgccg tcatcttcga tgtcgacggg acgttgtggg attccgcgcc gggaatcctc
    10321 agctgcttgg tcgagacctt cgagtacttc gacctcgaag tgcccggcga agttgcgctg
    10381 cgcgccgcgc tcgggccgcc gctggacatg atgcttcgcg acctcggctt tccgtccggt
    10441 cagatcgagg ccggtcgcct cgagtaccga cgccgatact tcgagcacgg cgaagccgag
    10501 tgcgaggtct acgccggcat tgttgagctg ctcgaacgac tcgccgcgaa tggacggtca
    10561 ctcgccgtcg ccacctcgaa gggacgcgaa accgcacacc ggatgttgga ggcgtttgga
    10621 ctcaccgagt acttcgactc catccgagcc gccgatatgg cgaccgccgc gcacggcaag
    10681 gtacacctca tcgccgcagc gctcaccgac ctcaacaccc gttcggcggt catggtcggc
    10741 gaccggaact tcgacatcga gggcggcctg tccaacgggc tctacaccat cggggtgaat
    10801 tggggatacg cccccaccgg cgagctcgaa gctgccggcg cccacgtcat cgtcgacacc
    10861 gtcagccaac tcgcctcggt tctcatcgac cggtaggttc acacgcgtgt ccgccatcgt
    10921 cgtcgaccac ctcagcgtcc gctacggcga cctcgtggcg gtacacgaca tcggattcgt
    10981 cgccgaaccg gccagaatca ccgtccttct tggcccaaac ggggcgggga agacctcgac
    11041 catcgaatgt atcgagggct atcgacggcc gagcggcggc acgatccgaa tcggcaacat
    11101 cgatcccttc accgaacgcc gggagttcgc ccgacgggtc ggcgtgatgt tgcaacacgg
    11161 cggaatccag cccgcggttc gaccgatgga gatcctgcgc ctgtacgagg cgctctacga
    11221 caatgcccac tccccggcgg acatgttggc gttcgtcggc ctgcaaaatc gggcgaagtc
    11281 cccgtggcga tcactgtccg gaggagaaca gcagcggttg gcgcttgcgc tcgccgtgat
    11341 cggacgccca caggtcgttt tgctcgacga accgaccgcc ggcgtcgaca tcgccggccg
    11401 caagctgatc cgcgacctca tcgccgacct ggcggcgcag ggcgtcaccg tgttgttgac
    11461 cacccacgac cttgcggaga tcgaagtcct cgcggacgac atcgtcatca tcgatcacgg
    11521 acgggtggtc gccgccgggt ccgcaagcga tctgctgtct ggagccgacg caccgtcgtt
    11581 tcggttccgc gcccaacccg gactcgacct cgccgcgctc agcgctgtgc tcggcgttgc
    11641 cgtcaccgac ctcggcgagg gcatgtacga cgtcgaagcg tccccgaccc cgccgctcgt
    11701 cgcgtcgttg acgggatggc tcgccgaacg ttcgatactg ctcggtgacc ttcaagccgg
    11761 ccgccagggg ctcgaacagc tcttcgtgcg cctcacctcc gagacggcgg gagccgaccg
    11821 tcgcagcgag tcggggcgcc gcacacgggc acagcgacgc tccccgcgga cgggacagcg
    11881 atgagagcgt tcaccgccca gctccgcctc gaattgatcc tcgccttccg caacggcgag
    11941 cagatcctcg tcaacctgtt gattccgcta ggactgctcg tcctcatggc aaacatcgac
    12001 atcttcgacg agtcgaccga accgattcaa cgcctcggac ccaccatttt ggccctcgcg
    12061 gtcatgagct cctcgctcgt gagtcttggg gtgggaaccg gtttcgaacg gttctacgga
    12121 gtgctgaagc ggctcggcgc cacgccgctc ggccgcggcc ggtgggtcgc cgccaaattc
    12181 ggatcgatca tgtgcatcga actcgttcaa tggtgcgtca tcgtcgtcgt cgccctggcg
    12241 ctggggtggt cccctcaagc atcggggtgg ttgccggcga tcgctgccgc tgcgctcgcc
    12301 acgtcggcgt tcggcgggct tgcattactc ctcgccggca ccctcaaggg gctcaccaac
    12361 ctcgcccttt gtaacgcgct ctacctcgtc ctgctcgtca ccggcggcat ggtcatccca
    12421 ctcgcagaga tgccgacccc catggcgacg gcagccaagg tctttccggc gggcactgtc
    12481 acagatcatg ctcggggcat tccatccggc ggaatcgatc ccttcgaacg cgtggctcgt
    12541 cctgatcgtc tgggccgtcg tgaccccgct gttggcgatt cgattcttcc gctgggagtg
    12601 atcgtcacca gaacgcccgc ccgcccgtgc gacgcgcaga gcggcacctt cgcggaaacg
    12661 acgaaacccg ccgctcaccg tgcaggtcat gcgcggtgtg cgacgggttt gtctcgaagc
    12721 gttctcgccc cggtcggccg acgagcctgc ccggacggtc agtgaccgcc ggtggtctcg
    12781 cggatgatcg gcggcggatc ggtcggaggc gggggcaggg gccgctgcat cgttggatcg
    12841 ttgaaggtga actcaccacc gaggccggac ttctcgaaga ttcgcatcga gccgaacacg
    12901 cccatgaaca ccgcaatagc gaggatcgcc ccgaaataga agagctgccc gagcagattg
    12961 tgatcgtcac ggaggtgcat gaagatctgc gccacgagaa tgaacttgat cgtcatcatg
    13021 acgatcagcg ctgcggtcgt gatccaaccg agcccctcgg gccaccattc ggtcgaaacc
    13081 tcaacggctg tcagaatcgc caacgcgacg aaggtcctcc aatacaggcc gtcgccgtgg
    13141 tcggcgtggt catcgtgatg ggcgtgtgtt gcgccaactg cagacatacg tcctcgctta
    13201 cttgatcagg tagacgatgg tgaacacgag aatccacacc acgtcgacga agtgccagta
    13261 cagcccgacg atttccaccg cctcggcctt cgcatgcgga atccggcctc gagcggacat
    13321 ggcgatcgtg atcagcaaca tcaagatgcc gatcgtgacg tgggcaccgt gaaagccggt
    13381 gagggtgtag aacgctgaag ccgcaacgtt cgaggtgtac cccatgccct ctcggacgaa
    13441 ggtcgtgaac tcgtacacct ggccgccgat gaacagcgag ccgagcactg cggtcgtgcc
    13501 gagccagtag cgcatgcgag ccatctcgtt gcgcttcgcg gcggcgacgg cgaagaccat
    13561 cgtcagcgag gacgccaaca agatgaacga agtgaccgac gtgaacccgt tggcgaacag
    13621 gtctgacggg cggatgtcgc ttcctgtgat ctcacggccg cgaaccgaca cgatggagcc
    13681 gggacggttc ttgtagagga agtaggtgga gatgaggcca ccgaacatca ggcattccga
    13741 tcccaggaac acccacatgc ccagcttctc attcgagatc ccggtattgg tcggcgggtg
    13801 gccgtgatcg tccaccgccg cgacgggcat ggtcagtgct tcactcacct gcgttctcct
    13861 cgctggcagc ggcgccaccg gcaacgccgg cgcctacggt ctcaagctca ccggcaacat
    13921 cggtggcggt gatggcttca ccatgatcgt cgtcgccgtc gtcgtgagcg tcgtgatgat
    13981 cgtgtcccgc atcgggatca gtcgacggct cgacgatcca tccggcgata ccggcaaccg
    14041 tcagaccagc gccgagaatc agccaccaat agctgaagat caggccgtag gcgatgaacg
    14101 gcagaccggc ggaaagcacg atcggccaat acgacggaga gggcagatgg atgtcgtcgc
    14161 ccgacacacg cttctgcacg atgtcgtcgg tctcggcgat gcggaccatt cgaccctgct
    14221 cgtcgtgacc gtacttgcga tgccagaaat catcgagctc gtggacgacc ggggtctccg
    14281 agaagttgta ctcaggggtc ggcgaaggag tgatccactc caagctgcgg gaatcccacg
    14341 ggtcggctcc cggatcgtat ggctcgcgct tccacttgcg ataggacacc cagcagttga
    14401 cgaggaactg cacgaggccg atggcgatca tgaacgcacc gatcgtcgcg accaggttcc
    14461 agaagttgaa cccgtagccg tcgctgtagg tgaaatagcg acggctcatg ccttggaggc
    14521 cgaggatgtg catcgggccg aaggtcaggt tgaacccgac cagcgtcatc cagaaatggg
    14581 ctttacccat gcgctcgtcg aggaagtggc cgaagatctt cggccaccag aaatacatgg
    14641 cgccgaagaa tccgagcagc gcgccgccga acagcacgta gtggaagtgg gccacgatgt
    14701 agtaggtgtc ggtctgctgg gtgtcggcag gggcgaaggc gtgcgtcaca cccgagaggc
    14761 cgccgatggt gaacatcgac accaacccca tcgcgaacat catcgcggcc gtgaaccgga
    14821 gccggccccc atacatggtg gcaacccagt tcagaatctt cacgccggtc ggcacggcga
    14881 tgaacatcgt cgacagcgag aacgccgcca tcgagatcgg c
//
LOCUS       DAOOMA010000005        14061 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_4684_length_14061_cov_4.314365, whole genome shotgun sequence.
ACCESSION   DAOOMA010000005 DAOOMA010000000
VERSION     DAOOMA010000005.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 14061)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14061)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14061
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4684_length_14061_cov_4.314365"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..537)
                     /locus_tag="PLP95_00315"
     CDS             complement(<1..537)
                     /locus_tag="PLP95_00315"
                     /inference="COORDINATES: protein motif:HMM:NF013286.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NifU family protein"
                     /protein_id="HPB44278.1"
                     /translation="MSTETTTAEERASGISPKVSPFAVEAPVIEISEAALVEVLRIRN
                     SEPDPESLALHVEITGASGTEYQYSLCFDELSAATDDDTIVETGEISVIVPNSSVDNL
                     RGSVLDLPRTAGQGGLVIRNPNRPNPLAGVELNLDGTIAEQVEELLEKAINPSLAAHG
                     GFAKLVGVDDENKVYITMG"
     gene            complement(701..2548)
                     /locus_tag="PLP95_00320"
     CDS             complement(701..2548)
                     /locus_tag="PLP95_00320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB44279.1"
                     /translation="MGEHKHLVRRRSIAGIAIAVSLAVAVSCIPPELALLARAAWPVA
                     AECGVADSFSCNLPYPSDRWLVEDASTATGVRLEIPTDVVPENVRSAFGDYDVLADAM
                     VNADGFSPVTPMAWQLPEGLARDAVPADGGELIQVWDATTGERVRVNVEVSNLQTDER
                     GVHNIIVAYPATRFEPGHRIFGGVVTGVAAVTGGLASASGSKAWASDATRNAAAQVTP
                     GIPWDQYLAATSFTVRSDESFTTDVDRMVGLIEAADHPMRNISVAPPVLGGALLVSGQ
                     IRSLDFRGEYGAIPRDGSAVGKERWLDFLLVLPQRPATAQGAPVIVYSHGLIASKETM
                     LVVADQNARKGFATIAIDKPNHGSRLQGGPIYDLIWPRNLPRVQSLLLQAVLDDVSLI
                     QAVKQHGAELDQYPANPVRGNGDGTPDLNPDHLYFAGTSLGGVLGAAAVGLAPDVDGA
                     YFQVPGSGIIDTLFHSLAWDLFHTLVPSAADYGDAHVLTFFAQTLLDRADNTHYLGRI
                     RERGIPMFLSYAIDDGLVRNQSTERMAEILGLPMVGDQRGPIPERLTQGALATMPADG
                     RGFGQIPTQQIPDTLLKRLLTHVQFIDPISVSMLDDWLDGRLAEIRSEG"
     gene            2640..3347
                     /locus_tag="PLP95_00325"
     CDS             2640..3347
                     /locus_tag="PLP95_00325"
                     /inference="COORDINATES: protein motif:HMM:NF004858.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crotonase/enoyl-CoA hydratase family protein"
                     /protein_id="HPB44280.1"
                     /translation="MSDTAIVTTRIDGDVAVVTLDDGKANAISFAALDQINAALDSAL
                     ETSKAVAFIGREGKFSAGFDLKVMTGEPESAREMLGRGAELGIRMAEFPIPLVLGVTG
                     HALAMGGIMTVTADYRVGGEGPFKLGLNEVAIGMPVPKFAVELCRARLQSSHFTRALQ
                     HAETFAPDPAVAAGFLDEVVELAQVESRAVEVAAHLAATLNPRAFALTRRFMRGAMIE
                     QIRAGLAEDLALFTVET"
     gene            complement(3363..5516)
                     /locus_tag="PLP95_00330"
     CDS             complement(3363..5516)
                     /locus_tag="PLP95_00330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB44281.1"
                     /translation="MVHRLGVRRGAAAVLLTSAMVISACSGGDGADSTSTTSTTSAVP
                     TATPMGAADLEVLRELVGDGACDELDPTHCLLPFPSNRFVVDDPSSDTGKRVSLPEGQ
                     LANTAGATLDPMEWNRNDGFSPNTPILFFAPDVDLDASGVATIGDIGDSMQDGSPVAV
                     VDLDSGERIPHWVEIDSHGAEDQRVVIIRPAVALDEGAHVGVIIGQLVDHSGQPIEAS
                     LATRAYRDNLTTDIDAVEDRRGEIDKVYAAADRLGYDRATMFAAWDFTVASQRSLSER
                     MLHIRDDGFERLGDAAPNFEVTNVVTTDLKPGIGRVVEGTFDVPLYLDGDGAAGSRFT
                     AREGDELPEYAGSDYRAEFACQIPEAAIAGSGATTRPVVYGHGLLGSLSEVKGSQVAK
                     NASMNNMMYCATNWIGMSSDDVGNAVEILGNISKFASLADRSQQGILNTLFLARVMGH
                     AGGFSSDAAFANTAGASVIDTSEVYFDGNSQGGIMGGAATAVSTEWAKAVLGVPGMNY
                     SLLLSRSVDFDTYFAVLRGAYPNELDQAILYPVLQMLWDRAESAGYAQHMTDDPYEGT
                     PEHQVLLHVGFGDHQVAQVAAEVMARTIGAGVREPALAEGRHPDAMPFFGLDMIPDAA
                     TERSLLVYWDSGTLPPPAANITPVSSDLWQSTCGAMAEEEWEHDARCADSHEDPRRAP
                     AAIQQKDAFFRPDGKITDTCDGRPCVAPNRFSLDY"
     gene            complement(5615..6604)
                     /locus_tag="PLP95_00335"
     CDS             complement(5615..6604)
                     /locus_tag="PLP95_00335"
                     /inference="COORDINATES: protein motif:HMM:NF018447.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase family protein"
                     /protein_id="HPB44282.1"
                     /translation="MGAGCPVGWAEPASTPTLSEARDVLTARRDARLAAETRLGELVS
                     DLANLDRSIASASDASAQLSASIESARAELRAAATNAFVDGGDTDPMLAYLSDWDLLV
                     TSRRLTFLEHRAGDLADASSQFEAMKRDNDPKLVAMAEARESLTARVTVAENALAQAN
                     ATEADAERALTAAEAKATADAAERAARAARAQPAGGAGAATSAASSPRPAPAAPNRND
                     PQPAAIPTTTTPRAVVATDAPSAPLPDLPEGGPTPEQWAALRQCESSGNYRAVSASGR
                     YRGAYQMDAGTWASLGGLGDPAAAPPIEQDARARLLHLRRGWKPWPHCGRHLQ"
     gene            6712..7581
                     /gene="mutM"
                     /locus_tag="PLP95_00340"
     CDS             6712..7581
                     /gene="mutM"
                     /locus_tag="PLP95_00340"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: protein motif:HMM:TIGR00577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HPB44283.1"
                     /translation="MIAIYATARSAERCLLAPYGMPELPEVETIRRQLEPQLVGARLS
                     GTWAFDSPKFLDAMLVEDQEVEALDRRGKYLITRLSSDVDLIVHLGMTGKVRVAPAGD
                     PHDPYSRARWRVDDDRIFSFDDVRRFGRIAVTTRDDHRALTTLHHLGPEPFDPTLDDE
                     RFWRSLRSTRRHIKTDLLSQRPIAGVGNIYADEALWRAGVHPADRTITRSKAYDLLDA
                     LRAVLAEGIEHGGTTLRDYVDAQGATGSHQHHLACYGRYDEPCERCGATLRRIVLDAR
                     TTTFCPSCQPRRR"
     gene            7680..9020
                     /locus_tag="PLP95_00345"
     CDS             7680..9020
                     /locus_tag="PLP95_00345"
                     /inference="COORDINATES: protein motif:HMM:NF014600.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HPB44284.1"
                     /translation="MVLPFHDALLDATFASNPDMNRRTPRTAARILAVSLALSLSLTP
                     LSIAGPSAAADSGSARQKLKELQSAQEKLRAEKSKSASNVNALQATEADVQASLASLR
                     ANVESEASLLEDSRRAVTAAEAREQEALAQEATAAAELEQLNADIKEQVRLAFIAGSS
                     VPDDPIEYLAEDSLTEASRKRVFRNANATSGADAAEHYRTIQNDLRIAREAAAAASAE
                     ATAKRAEVETRLAKLQDAQAAQEAYADQVADRIANAYAEAAGLEALEPALAEQIKGQQ
                     QALDSALASEREAAAARARAAGARTTSGRPSGSGGSVSPVGEGSIVAVGGIRVHRDIA
                     DSLAALLNAAAADGIHLSGGGYRDSAGQIATRRNNCGTSNYAIYEMPSSSCRPPTARP
                     GSSMHERGLAIDFSQNGRVLNRGSSAFSWLKANAANYGFYNLPSEPWHWSTNGN"
     gene            complement(9062..10567)
                     /locus_tag="PLP95_00350"
     CDS             complement(9062..10567)
                     /locus_tag="PLP95_00350"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HPB44285.1"
                     /translation="MFDAIEDGMGERRPTSEPLSARADRQPQRRWSLHAKLGLIAAVG
                     LGAALGAAGAAFVSPIAALVVALIGSVITVVVARAVAESIGRSLLDVGRQMRRFGDGD
                     VSVRMAPATDELGTVARSFNVMADATSERIVSLADSARDAIHLRSVADALEMARDEDD
                     VYRIVEQSVALLTPHNPAQLLVADSPTRFRSAAQHPSAGSPGCPVSNVDDCPAMRRAQ
                     TMTFQGPSELNSCPLLAEHEEVCSAVCVPVGVNGHLLGVLHVAGREGDPPKPTTVDQL
                     VSLAGVVGSRIGSIRLLESSRLEASTDGLTGLANRRKFERAAGDLLRDRVPFSLVMAD
                     IDHFKRLNDNFGHETGDRALQLFAGVLQDNVRGHDIVARTGGEEFVLVYPNLAPKRTI
                     EVLRRIQIALAAALDRAGLPKFTASFGVTDSSVAETLDDIVRIADAGLIVAKQSGRNR
                     IELADNGLASSVFSREAKDAPGEPLIDLSTSPTRSPITSGRDHDHSLSENG"
     gene            complement(10696..11628)
                     /locus_tag="PLP95_00355"
     CDS             complement(10696..11628)
                     /locus_tag="PLP95_00355"
                     /inference="COORDINATES: protein motif:HMM:NF012472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HPB44286.1"
                     /translation="MDARTDSTAEDDAVELSALGLSTSRIYELDVSAAAELLNAAVES
                     GCTVIDCADVWGFDPAALADPSQRPGFGSAEERIGEVLATSPGLRDELFITTKGGAFP
                     PIPLECSADYLVQACDASLYRLGVEQIDLYQIHQPDLLTHPAEVASALDSLVAAGKVA
                     QVGVCQHNPSQVDTLQAFLDVQIASVAMPFNLLALGALIDGRLDQAVTLGLVPFATQP
                     LCGGRVFGELTGKTAAAVEVCRRIADEQGVELAAVLLAFAMHHPSTMVPLVSTISVDH
                     LRQAAAAEDVGLSRQQWYEIYQAARGSQILHGMP"
     gene            complement(11672..12724)
                     /locus_tag="PLP95_00360"
     CDS             complement(11672..12724)
                     /locus_tag="PLP95_00360"
                     /inference="COORDINATES: protein motif:HMM:NF012770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HPB44287.1"
                     /translation="MNPFSVNPNPGSSPPPPPQGPRRPFASGSRTAIWVGVGAASVVA
                     VLALVIALTNGQQAAAPAATSTTQVETTLAPTPTSTPVTEPPTTRPVSTELHRESCVL
                     HELVARGCDLMVAQDAVGTGPLPVVILLHGLAGSESAVRSAGAWPDAVASHRFMLVTP
                     AGVAGSWNAQGCCGVASAVQIDDVGFLRGLIERLTARDDVDASRIYLVGHSNGGMMAY
                     RYLCSDAERIAGMVSVSGTRVSGCFPSAPTDVLQIHGTGDTTVPYDGGQGLVAAILGV
                     TFSSVPSTMQQMAEAQQCALDPQVVESDPVTEQSWTGCSGGAHVDLWSVRGLNHDWMR
                     KPVNLTDRILDYFGID"
     gene            12808..14004
                     /locus_tag="PLP95_00365"
     CDS             12808..14004
                     /locus_tag="PLP95_00365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HPB44288.1"
                     /translation="MLVGDSTADSEFRDEIRTWMHENLTGDFAKLRFRGGPGDEHMFV
                     QERMEWERHLASARWTCVGWPTQYGGRGLPISQEVIFHEEYARAGGPGRAGLIGEGLL
                     GPTLIHFASEEQKAQFLPGIVGGTEYWCQGYSEPNAGSDLANIKTTARLEGEGVDGQW
                     VINGQKVWTSLAQWSDWCFVIARAEEGSQRHAGLVYLLVPMTAEGIEVRPIRQITGTA
                     EFNEVFFDDVRTDASNIVGGIGDGWKVAMGTLAFERGVLTLGQQMAFQRELTEIIDAA
                     KANGRLEDPNLRDAITSLWIRVRIMRANAVRTLSLAESQLGREAMITKLFWASLHRDM
                     GEVAMDAMGSEAMIINDSYDSHEPLEALHRLFMFTRSDTIYGGSNQVQRNVIGERALG
                     LPREPR"
BASE COUNT         2436 a         4804 c         4568 g         2253 t
ORIGIN      
        1 acccatggtg atgtacacct tgttctcgtc gtcgacgccg acgagtttcg cgaacccacc
       61 gtgggccgcg aggctggggt tgatggcctt ttcgagtagc tcctcgacct gttcggcaat
      121 ggtgccatcg aggttgagtt cgacgccggc cagcggattg ggccggttcg ggttgcgaat
      181 aaccagccca ccctgacccg cggtgcgcgg cagatcgagc accgagcctc gcaggttgtc
      241 gaccgagctg ttcggcacga tgaccgagat ctcgccggtt tcgacgatcg tgtcgtcatc
      301 ggtcgctgcg gagagttcgt cgaaacacaa cgaatactga tactcggtgc cgctcgcgcc
      361 ggtgatctcg acgtggaggg ccaacgattc ggggtcgggc tccgagttgc ggatgcgcag
      421 cacctccaca agcgcggcct cgctgatctc gatgacgggt gcttcgacag cgaacggact
      481 caccttgggt gagattccag aggcacgttc ctcggctgtg gtggtctcag tggacactgc
      541 gttctccttt ggcgccgtcc aagggcaccc gacaagggta gcttccggcc tgccaaccgt
      601 cagaacccgc gtcggcccgc gaacattccc cgtcgcctcg cggcgacgtc gccgcagcac
      661 agcgcggcga cgtcatctcc ggcggttccg cagcctcgga tcaaccctcg gaacggatct
      721 cggcgagtcg accgtcgagc cagtcgtcaa gcatcgacac cgaaatcggg tcgatgaact
      781 gcacgtgggt cagcagtcgc ttcagcagag tgtctggaat ctgctgggtg ggaatctggc
      841 cgaacccgcg cccgtcggcc ggcatcgttg cgagcgcgcc ctgcgtcagg cgctcgggga
      901 tcggccctcg ctggtcgccg accatcggca ggccgagaat ctccgccatt cgctcggtcg
      961 actggttgcg caccagaccg tcgtcgatcg catagctcaa gaacatcggg atgccgcgtt
     1021 cgcgaatccg gcccaggtaa tgggtgttgt cggcacggtc cagaagcgtc tgggcaaaga
     1081 aggtcagcac atgggcatcg ccgtaatcgg cagccgacgg aacgagggtg tggaacagat
     1141 cccacgccag ggagtggaac agggtgtcga taatccccga gccgggaacc tggaagtacg
     1201 caccgtcgac atccggggcc aacccgacgg ccgcggcgcc aaggacgccg ccgagcgagg
     1261 tgccggcgaa gtagaggtga tccgggttga ggtcgggggt gccgtcgccg ttgccccgca
     1321 cagggttcgc cgggtattga tcgagttccg caccgtgttg tttgaccgct tggatgagcg
     1381 acacgtcgtc gagcaccgcc tggagcagca acgattgcac ccgggggaga tttctcggcc
     1441 agatcagatc gtaaatcggc ccaccctgca gccgggaccc gtggttcggc ttgtcgatgg
     1501 cgatggtcgc gaaccccttt cgagcgttct gatccgcgac caccagcatc gtctctttcg
     1561 aagcgatgag tccgtggctg tagacgatga ccggggcgcc ctgcgccgtc gccggccgtt
     1621 gcggcaacac caacaggaaa tcgagccaac gctccttgcc gaccgccgac ccatcccgag
     1681 ggatggctcc gtactcgcca cggaagtcca gggaacggat ctgacccgaa accagcagtg
     1741 ccccgccgag caccggcggc gccaccgaga tgttgcgcat cggatggtcg gccgcttcga
     1801 tgaggccgac catccggtcg acatcggtag tgaacgattc gtcagaccgt acggtgaacg
     1861 acgtcgctgc caggtactga tcccaaggaa ttcccggggt gacctgtgcc gcggcgtttc
     1921 gggtagcgtc cgacgcccac gctttcgaac cggatgcgct cgcgagccct ccggtgacgg
     1981 cggcaacccc cgtcacaacg ccaccgaaaa tgcgatgccc cggttcgaat cgggtggcgg
     2041 ggtaggcgac gatgatgttg tgcacgccgc gctcgtcggt ttggaggttc gatacctcaa
     2101 cgttgacccg cacccgctcg ccggtcgtcg cgtcccacac ctggatgagt tcgccgccgt
     2161 cggccggaac ggcatccctc gcgagccctt cgggcagttg ccaagccatc ggggtgacgg
     2221 gggagaatcc gtcggcgttc accatcgcgt cggccaacac gtcgtagtcg ccgaacgccg
     2281 aacgcacgtt ctcggggacc acatccgtcg ggatttccaa ccgcacgccc gtggcggtcg
     2341 acgcgtcctc gaccagccag cgatcgctcg ggtaggggag gttgcacgag aacgagtcgg
     2401 cgacgccaca ctcggccgcg acgggccacg cggcgcgtgc cagcagcgcc agctccggcg
     2461 gaatgcaact gaccgcaacc gcgagcgaaa ctgcgatcgc gatgcctgcg atgctgcgac
     2521 gacgaaccag atgcttgtgt tcccccatgt cggcgcactg tacgaggccc acgccgaccg
     2581 gcacaagtcg ctgtttcgct tccgatgccg ctcaccgcga cccttgtagg gttggaccca
     2641 tgtctgacac cgcgattgtc accacccgaa tcgacggcga tgtcgccgtc gtcaccctcg
     2701 acgacggcaa ggcgaacgcc atttcgttcg ctgcgctcga ccagatcaac gccgcgctcg
     2761 attccgcgct cgaaacctcc aaggccgtgg ccttcatcgg ccgcgagggc aagttctccg
     2821 ctggtttcga cctcaaggtc atgaccggag aacccgagtc cgcccgcgag atgcttggca
     2881 ggggcgccga gttgggcatc cgcatggccg agttccccat cccgctcgtc ctcggcgtga
     2941 cgggccatgc gttggcgatg gggggcatca tgaccgttac cgccgactat cgggtcggag
     3001 gcgagggtcc gttcaagctc gggctcaacg aggtcgccat cggcatgccg gtgccgaagt
     3061 tcgccgtcga actgtgccgt gcacggttgc agtcctcgca tttcacccgg gcgctccagc
     3121 acgccgagac cttcgccccc gaccccgccg tggccgccgg gtttctcgac gaggtggtcg
     3181 aactcgcgca ggtcgaatcg cgcgcggtcg aggtggcggc ccacctcgcc gccaccctca
     3241 atccccgagc gttcgccctc acccgacgat tcatgcgtgg cgcaatgatc gagcagatcc
     3301 gcgccgggct ggccgaggat cttgccctgt tcaccgtcga gacctgacgt cgaccagacc
     3361 gatcagtagt cgagcgagaa ccggttcggg gcgacgcacg gccggccgtc gcacgtgtcg
     3421 gtgatcttgc cgtcgggccg gaagaacgca tccttctgct gaatcgccgc gggcgcccgt
     3481 cgcgggtcct catgggaatc ggcgcagcgc gcgtcgtgtt cccattcctc ctccgccatc
     3541 gcgccacagg tcgactgcca caggtccgaa ctcaccggcg tgatgttcgc tgcgggcggc
     3601 ggcagggttc cgctgtccca gtacaccagc agcgaccgct cggtcgccgc gtcgggaatc
     3661 atgtcgaggc cgaagaacgg catggcgtcg gggtggcgtc cctccgcgag cgccggctcg
     3721 cggactccgg cgccgatcgt gcgcgccatc acttccgcgg ccacctgggc gacctgatga
     3781 tcgccgaagc cgacgtgcaa caacacctgg tgttcgggcg taccctcgta gggatcatcg
     3841 gtcatgtgtt gcgcatagcc ggccgactcg gcgcggtccc acagcatctg caggaccgga
     3901 tagaggatcg cctggtcgag ttcgttcgga taggcaccgc gcaacacggc gaagtaggtg
     3961 tcgaagtcaa cgcttcggct gagcagcaac gagtagttca tgcccggcac accgagcacc
     4021 gccttggccc attcggtgga caccgccgtt gctgcgccgc ccatgatgcc gccctggctg
     4081 ttgccgtcga agtacacctc ggaggtgtcg atcaccgagg ctcccgcagt gttggcgaac
     4141 gccgcgtcgg agctgaaccc gcccgcgtga cccatcaccc gagccaggaa caacgtgttc
     4201 aagatgccct gttgcgaccg gtcggccagg ctcgcgaact tggagatgtt gccgaggatc
     4261 tcgacggcat tgccgacatc gtcgctgctc atgccgatcc agttggtcgc gcagtacatc
     4321 atgttgttca tcgacgcgtt cttggcgacc tgcgacccct tgacctcgct cagagaaccg
     4381 aggaggccgt ggccgtagac gacggggcgt gtcgtcgccc cactcccggc gatggcggcc
     4441 tcgggaatct ggcaggcgaa ctcggcgcga tagtcgcttc ccgcgtactc cggaagctcg
     4501 tcgccctctc ttgcggtgaa acgggatccg gcagcgccat caccgtcgag gtagagggga
     4561 acatcgaagg tgccctcgac gacgcgaccg atcccgggtt tcaagtccgt cgtcaccacg
     4621 ttggtcacct cgaaattcgg cgccgcgtca ccgagtcgtt cgaagccgtc gtctcgaatg
     4681 tgcaacatgc gctccgacag gctgcgttgt gaggccacgg tgaagtccca cgccgcgaac
     4741 atggtggccc ggtcgtatcc caaccgatcg gccgcagcgt agaccttgtc gatctcacca
     4801 cgacgatcct cgaccgcgtc gatgtcggtc gtcagattgt cgcgatacgc ccgggtcgcc
     4861 agcgacgctt cgatcggctg ccccgaatgg tcgacgagtt ggccgatgat cacgccgacg
     4921 tgcgcgccct cgtcgagcgc gacggccggt cggatgatga cgacccgctg gtcctcggcc
     4981 ccgtgggagt cgatctcgac ccaatgaggg atccgttcgc ccgagtcgag atcgacgacc
     5041 gctaccggag agccgtcctg catcgagtca ccgatgtcac cgatggtggc gacgccggag
     5101 gcgtcgaggt cgacgtcggg agcgaagaac aggatcggcg tgttggggct gaatccgtcg
     5161 ttgcgattcc actccatcgg atccagggtg gcgccagcag tgttcgccaa ctgcccctcg
     5221 ggcagcgata cccgctttcc ggtgtcgctc gacgggtcgt cgaccacaaa cctgttggac
     5281 gggaagggca acagacagtg cgttgggtcg agttcgtcgc aggcgccgtc gccgacgagt
     5341 tcacgcagca cttcgaggtc ggcggcaccc atcggggtcg ccgtcggcac ggccgaagtc
     5401 gtcgaggtcg tcgaggtcga atcggccccg tcgccgccgc tgcacgcgga gataaccatc
     5461 gctgacgtca gcagaaccgc agcggcccca cgccggaccc caagtctgtg gaccatgtcg
     5521 actcctctcc tgcggccggt cgcaccggtg tcgatcggcc ttggacgcgg aatccccccg
     5581 tcgcccccga ccacactacg caggagcgcg gctgctactg gagatgccgc ccacagtggg
     5641 gccacggctt ccagccgcgg cgcaggtgca gcagcctcgc ccgagcgtcc tgttcgatcg
     5701 gaggcgcagc cgcggggtcg ccgagcccac ccaacgatgc ccacgtaccg gcatccatct
     5761 ggtaggcgcc gcggtagcga ccgcttgccg agaccgcccg ataattgcca ctcgattcac
     5821 actggcgaag cgccgcccat tgttccgggg tcgggccgcc ttcggggagg tcgggaagcg
     5881 gtgcggacgg ggcatcggtc gcgaccaccg ctcgcggtgt cgtcgtcgtg gggatcgctg
     5941 ccggctgggg atcgttgcgg ttcggggctg caggcgccgg tcggggtgac gaagcggcgg
     6001 acgtcgcggc acccgcgccc cccgccggct gtgcccgggc ggcccgtgcc gcacgctcag
     6061 cggcatcggc cgtcgccttc gcctcggcag cggtcagagc tcgctcggcg tcggcttccg
     6121 tcgcgttggc ctgggcgagc gcattctcgg caacggtcac gcgggcggtg agcgattcac
     6181 gcgcttcggc catcgcgacc agcttggggt cgttgtctcg tttcatggcc tcgaactgcg
     6241 acgaggcgtc ggccaggtcg ccggcacggt gctccaggaa ggtcaaccgc ctcgaagtga
     6301 ccagcagatc ccagtcggag aggtacgcga gcatcgggtc ggtgtcgccc ccgtcgacaa
     6361 acgcgttggt ggccgcagca cgcaactcgg ctcgggcgga ctcgatcgat gcgctgagct
     6421 gtgccgaggc gtctgatgcc gaggcgatcg aacggtcgag gttcgccaga tcggagacga
     6481 gttcgcccaa ccgcgtttcg gcggccaacc gggcgtcacg acgcgccgtg agtacgtcgc
     6541 gagcctcgct cagcgtcggc gtcgatgctg gctccgccca cccgacgggg caaccagcac
     6601 ccatcacgac gcaggcgacg gcaccagcag cgacccctcg tacgccgagc gatcgccgag
     6661 aagcgctcga gcctcgctcg gaccatccca tcacgatcag agctttgtca catgatcgca
     6721 atatatgcaa ccgcgcgttc ggccgagcga tgcctgctgg caccatatgg catgcccgaa
     6781 ctccccgagg tcgaaaccat ccgccgccag ctcgagccac agctggtcgg ggcccggttg
     6841 agtggcacct gggccttcga cagccccaag ttcctcgacg cgatgctggt tgaggaccag
     6901 gaggtcgagg cgctcgaccg tcgcgggaag tacctgatta cccggctctc gagcgacgtc
     6961 gacctgatcg tccatctcgg catgaccggc aaggtccggg tcgcaccggc gggcgacccc
     7021 cacgacccgt attcgcgagc gcgctggcgg gtcgacgacg accggatctt ctcctttgac
     7081 gacgttcgac ggttcggacg catcgccgtg accacgcgcg acgatcatcg ggccctgacg
     7141 accctgcacc acctcggccc tgaaccgttc gatccgaccc tcgacgacga acggttctgg
     7201 cgatcgctgc gttcgacgag acgccacatc aagaccgacc tgctgtcgca gcggccgatc
     7261 gccggggtcg gcaacatcta cgcggacgag gcgctgtggc gcgccggggt ccacccggcc
     7321 gatcgcacga tcacccggtc gaaggcctac gacttgctcg acgcgctacg cgctgtactc
     7381 gccgaaggaa tcgaacacgg cggaacgacg cttcgcgact acgtcgacgc gcagggcgcg
     7441 accggcagcc accaacacca tctcgcttgc tacggccgct acgacgagcc gtgcgagcgt
     7501 tgcggagcaa cgctacgacg aatcgtcctc gacgcacgca cgacgacgtt ttgcccgtcg
     7561 tgccaaccac gtcggcgcta gcggtgccgc cgatccgccg atccgtcgac cttggttcct
     7621 gctcgccacg gtgacgactc acagattcga cggttggccg accggcgtcg tccgacacgg
     7681 tggtgttacc gtttcatgac gcgttgctcg acgcgacctt cgcgtccaac cctgacatga
     7741 atcgccgtac cccccgcact gcagcccgaa ttctggcggt gagtctcgcc ctttcgctga
     7801 gcctcacacc gctgtcgatc gccggtccct ccgccgccgc cgactccgga tcagcgcgtc
     7861 agaaactcaa agagcttcag tcggcacagg agaagctgcg agccgagaag tcgaagagcg
     7921 cctccaacgt caatgcgctg caggcgaccg aggccgacgt ccaagcgtcg ttggcctccc
     7981 tgcgcgccaa cgtcgaaagc gaagcgtccc tcctcgagga ttcgcgtcgc gcggtaaccg
     8041 cggccgaggc gagagaacag gaggccctcg cccaggaggc gaccgcggct gccgaactcg
     8101 aacagctcaa cgccgacatc aaggaacagg tccgtctggc gttcatcgcc gggtcgtcgg
     8161 tgcccgacga cccgatcgag tatctcgcgg aggactcgct caccgaagcg tcccgcaagc
     8221 gggtcttccg gaacgcgaat gccacgagtg gagccgacgc ggcggagcat taccggacga
     8281 ttcagaacga cctccgtatc gcgagggagg ccgctgcagc cgcctcggcc gaggccaccg
     8341 ccaaacgtgc ggaggtcgaa acacggttgg ccaaactgca ggacgctcag gcggcccagg
     8401 aggcctatgc ggaccaggtg gcggaccgta tcgccaacgc ctacgcggag gccgccggac
     8461 tcgaagcgct cgaaccggcg ctcgccgaac agatcaaagg ccaacagcag gcgctcgact
     8521 cggcccttgc ctccgagcgc gaggccgcag cagcgcgggc gcgagccgcc ggcgcccgca
     8581 cgacgagtgg ccggccctcg ggttccggcg gctcggtctc cccggtcggc gagggcagca
     8641 tcgtggccgt cggcggcatc cgcgtccacc gcgacatcgc cgatagtctc gcagcgttgc
     8701 tgaacgccgc cgccgccgac gggatccatc tctccggtgg cgggtaccgc gactccgccg
     8761 ggcagatcgc cacgcgtcga aacaactgcg gcacgagcaa ctacgccatc tacgagatgc
     8821 cgtcttcctc gtgtcgcccc ccgaccgccc gacccggaag ttccatgcac gagcgtggcc
     8881 tcgcgatcga tttcagccag aacggcaggg tgttgaaccg agggagctca gcgttcagct
     8941 ggctcaaggc caacgcggcc aactacggct tctacaacct cccgtcggaa ccctggcact
     9001 ggtcgaccaa cggtaactga cccccaagtt tcagcgagtt tcggcggctt tcagcgacca
     9061 ttcacccgtt ctccgataac gagtggtcgt gatcacgacc actcgttatc ggagaacggg
     9121 tcggggaggt cgacaagtcg atcagcggct cgcccggggc atccttggcc tcgcgagaaa
     9181 acaccgacga cgccaacccg ttgtcggcga gttcgatgcg gttgcggccg gactgcttgg
     9241 ccacgatgag tccggcgtcc gcgatccgaa cgatgtcgtc caacgtctcc gcgactgaag
     9301 agtcggtcac cccgaaactg gcggtgaact tgggcaatcc agcccgatcc aacgcggccg
     9361 cgagcgctat ctggatccgc cgaagcacct cgatcgtgcg cttcggcgcc agattcggat
     9421 acacgaggac gaactcctcc ccgccggttc gcgccacaat gtcatggcca cgcacattgt
     9481 cctgcaacac gccagcgaac agttggagcg cacggtcgcc ggtctcatga ccaaagttgt
     9541 cgttcagccg cttgaaatga tcgatatcgg ccatcaccaa cgagaagggg acgcggtctc
     9601 gcagcaggtc gccggctgcc cgctcgaatt tgcgtcgatt ggccagcccg gtgagcccat
     9661 cggtgctcgc ttccaagcgc gaggactcga gcaggcggat ggatccgatc cgcgacccga
     9721 ccacgcccgc cagcgagacg agttgatcca ccgttgtcgg cttcggcggg tcgccttcgc
     9781 gcccggcgac gtgcagcacc ccgaggaggt gtccgttcac accaaccgga acgcacacgg
     9841 cggagcagac ctcttcatgt tccgccagca gcggacagga gttcagctct gatggcccct
     9901 ggaacgtcat cgtctgcgca cggcgcattg cggggcagtc gtcgacgttc gacaccgggc
     9961 accccggcga gccggcactc gggtgttgcg ctgccgaccg aaatcgcgtc ggcgaatcgg
    10021 cgacgagaag ctgggccggg ttgtggggag tcagcaacgc aacggattgt tcgacgatgc
    10081 ggtagacgtc gtcctcgtcg cgggccatct cgagcgcgtc ggccactgaa cgcaggtgga
    10141 tcgcgtctcg ggcggaatcg gcgagcgaca cgattcgctc gctggtcgcg tcggccatca
    10201 cgttgaacga ccgggccacc gtgccgagtt cgtcagttgc cggagccatg cgtaccgaca
    10261 cgtcgccgtc gccgaagcgg cgcatctggc ggcccacatc gagcagcgag cgaccgatcg
    10321 actccgccac cgctcgcgcg acgacgaccg ttatgaccga cccgatgagg gcgacgacca
    10381 gcgctgcgat agggctgaca aacgccgccc ccgccgcgcc gagcgcggcg ccgaggccga
    10441 ctgccgcaat caggccgagc ttggcatgaa gcgaccaacg gcgttgcggt tggcggtccg
    10501 cccttgcgga cagtggctcg gatgtcgggc gccgctcacc cattccgtcc tcgatcgcat
    10561 caaacatgtc ggtcttccgt gcccctgtcg ctcgaacgcc acaaccggcc gatcgacagt
    10621 ggaaattaca agcgtgtcat cgtacctcag ccgagaggga atctcggtgc ggcccaggta
    10681 ccttgcaaca tttcgtcacg gcatgccatg gagaatttgg gaacctcgcg ccgcttggta
    10741 gatctcgtac cactgctggc gcgacaaacc gacgtcctcc gccgcagcgg cttgccgcag
    10801 gtggtccacg gaaatcgtcg acaccagcgg caccatcgtc gaggggtgat gcatggcgaa
    10861 cgccagcaag actgcagcga gttcgacacc ctgctcgtcg gcaatccggc ggcacacctc
    10921 gaccgccgct gcggtcttgc ccgtcagttc tccgaaaacc cgaccgccgc acagcggctg
    10981 ggtcgcgaag ggaacgaggc ccagggtcac cgcctggtcg agccggccgt cgatcaacgc
    11041 tccgagcgcc aggaggttga acggcatggc gaccgaggcg atctgcacgt cgagaaacgc
    11101 ctgcaacgtg tcgacctggg aggggttgtg ttgacacacc cccacctggg ccaccttgcc
    11161 ggcagcgacg agtgagtcga gtgcggaggc cacctcagcg gggtgggtga gcaggtcggg
    11221 ttgatgaatc tgatacagat cgatctgctc aacccccaag cggtacaacg acgcgtcgca
    11281 cgcctgcacc aggtagtcgg ctgagcactc cagcggaatc ggaggaaacg caccgccctt
    11341 ggtggtgatg aacagctcgt cgcgaagccc cggtgatgta gcgagcactt cgccgatccg
    11401 ctcctcggcc gacccgaatc cgggacgctg agacgggtcc gcaagtgccg ccgggtcgaa
    11461 cccccatacg tcggcgcagt cgatcaccgt gcaccccgac tcaacggcag cattcagcaa
    11521 ctcggcggct gcgctcacgt cgagttcgta gatccgggag gtgctcagcc cgagggcgct
    11581 cagctcgacg gcgtcgtctt cagcggttga gtcggtccgg gcatccatcg aggtacggta
    11641 ccgcgcgagc acgcgaccgc tggcgctcgg ctcagtcgat gccgaaatag tcgaggatgc
    11701 gatcggtcag gttcaccggc ttgcgcatcc agtcgtggtt caacccacgg acactccaca
    11761 ggtcgacatg cgcaccaccg ctgcagccgg tccacgactg ttcggtgacc gggtcgctct
    11821 cgacgacctg aggatcgagc gcacactgct gggcctcggc catctgttgc atcgtcgaag
    11881 ggaccgagga gaacgtcacg ccgaggatcg ccgcaaccag gccctgtccc ccgtcgtagg
    11941 gcacggtcgt gtcgcccgtg ccgtggatct gcaagacatc ggtcggcgcc gacgggaagc
    12001 acccgctgac acgggttccg gaaacgctca ccatcccggc gatgcgttcg gcatcggaac
    12061 agaggtagcg atacgccatc atcccgccgt tggagtgtcc gacgaggtag atccgcgacg
    12121 cgtcgacgtc gtcacgtgcg gtgagtcgct cgatgagccc gcgaagaaag cccacgtcgt
    12181 cgatctgcac cgcgctcgcg accccacagc acccctgcgc gttccagctg ccggcgaccc
    12241 cggcgggggt gacgagcatg aagcgatggg atgcgaccgc gtcgggccat gcgcccgcac
    12301 ttcgcaccgc cgattccgat ccggcgaggc cgtgcagcag gatcaccacc ggcaacggcc
    12361 cggtgccgac ggcgtcctgg gccaccatca ggtcgcaacc gcgtgcgacg agttcgtgca
    12421 acacgcagga ctcccggtgc agttccgtcg acaccggccg agtcgtcggc ggttcggtca
    12481 ccggcgtgga cgtcggcgtg ggggccagcg tcgtttccac ctgcgtcgtt gaggtcgccg
    12541 ccggtgcagc cgcttgctga ccgttcgtca acgcaatcac cagtgcgagc accgcgacca
    12601 ccgacgcagc gccgacgccc acccagatcg ccgtccgcga tcccgacgcg aacggccgcc
    12661 gcggtccctg cggtgggggc ggcggcgacg agcccgggtt ggggttgacg gagaacggat
    12721 tcacggcctg cgagcgtaga ggctgcgact gcggctgccg gctcgcggat gggaagatct
    12781 gacacctcgt cagatacgat tcgacccatg ttggttggcg attcgacagc agactccgag
    12841 ttccgtgacg agatccgcac ctggatgcac gagaacctga ccggcgactt cgccaagctc
    12901 cggttccggg gcggccccgg cgatgagcac atgtttgtcc aggagcggat ggagtgggaa
    12961 cgccacctcg cctcggcccg ttggacctgt gtcgggtggc caacccagta cggcggccgc
    13021 gggctcccga tcagccagga agtcattttc cacgaggaat acgcacgagc gggaggaccg
    13081 gggcgcgccg ggctcatcgg cgagggcctc ctcggcccga cgctcatcca cttcgcaagc
    13141 gaggaacaaa aggcgcagtt cctccccggc atcgtcggcg gcaccgagta ctggtgccag
    13201 ggctactccg aacccaacgc cggaagcgac ctcgccaaca tcaagacgac cgctcgcctg
    13261 gagggcgagg gcgtcgacgg ccagtgggtc atcaacggcc aaaaggtgtg gacctcactc
    13321 gcccagtggt cggactggtg ttttgtcatc gcccgcgccg aggagggtag ccagcgccat
    13381 gccgggctcg tctatctcct ggtgccgatg accgccgagg gcatcgaggt ccgcccgatc
    13441 cgccagatca ccggcaccgc cgaattcaac gaggtgttct tcgacgacgt tcgcaccgat
    13501 gcctccaaca tcgtcggcgg catcggcgac ggctggaagg tggcaatggg caccctggcg
    13561 tttgagcgcg gcgtgctcac gctcggccag cagatggcct tccagcgcga gctcaccgag
    13621 atcatcgacg cagcgaaggc caacggccgc ctcgaggacc ccaatctgcg cgatgcgatc
    13681 acctcgctgt ggatccgggt ccgcatcatg cgagcgaacg ccgtgcgcac gttgtcgctc
    13741 gctgaaagcc aactgggccg cgaggcgatg atcaccaagt tgttctgggc ctcgctgcac
    13801 cgagacatgg gcgaggtggc gatggacgcg atgggctcgg aggccatgat catcaacgac
    13861 agctacgaca gccacgaacc cctcgaggcg ctgcatcgcc tgttcatgtt cacgcgatcc
    13921 gacacgatct acggcggctc gaaccaggta cagcgcaacg tcatcggcga gcgggcgttg
    13981 ggcctaccgc gcgaaccccg ctaaacgcac gacgtctgcg cacagtccgc ttccgttctc
    14041 gaccgtctca cgacccgccc g
//
LOCUS       DAOOMA010000006        14038 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_4704_length_14038_cov_4.433956, whole genome shotgun sequence.
ACCESSION   DAOOMA010000006 DAOOMA010000000
VERSION     DAOOMA010000006.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 14038)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14038)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14038
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4704_length_14038_cov_4.433956"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..490)
                     /locus_tag="PLP95_00370"
     CDS             complement(<1..490)
                     /locus_tag="PLP95_00370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020378422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HPB44289.1"
                     /translation="MVDVPSFDSLFIGGKWVAPASDRRFELRSPSTGELLASAPEAVE
                     ADVDAAIAAARDAFDNGPWPRMSAAERGAIMDRFYDLFIEHSNELAELITAEVGSPLL
                     FSHFGQVGASGMTLDFFRNLVKSYEFEQVRDGMLGPVLVRREPVGVCAGIIPWNVPIF
                     ITM"
     gene            complement(574..879)
                     /locus_tag="PLP95_00375"
     CDS             complement(574..879)
                     /locus_tag="PLP95_00375"
                     /inference="COORDINATES: protein motif:HMM:NF016839.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HPB44290.1"
                     /translation="MVIAVSVLISPTRRLMAQSRDMAVAEQQLVEVKSENRRLSERVS
                     ALSSDSLIEMEARERFGRVYPGDESYSVPESGPIELHLPEVWPFTRVDEALREAAAG"
     gene            complement(921..2210)
                     /gene="eno"
                     /locus_tag="PLP95_00380"
     CDS             complement(921..2210)
                     /gene="eno"
                     /locus_tag="PLP95_00380"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HPB44291.1"
                     /translation="MSIIEHIHGRQILDSRGNPTVEVEVLLTTGAAGRAAVPSGASTG
                     QFEAVELRDGGSDYLGKAVTKAVEHVNDDIAEAIVGLDALDQRQIDRILLDVDGTHNK
                     GRLGANAILGVSLALARAAAEEVGLPVFRYVGGANAHVLPVPMMNVLNGGEHADNNVD
                     LQEFMIMPVGAGSFSEALRWGTETYHVLKNVLHDKGLSTAIGDEGGFAPNLASNEEAV
                     QLLIAAIEKAGYEPGEQIAIALDAASSEFYRDGNYVLAGEGRTLNPSEFAGYLAELTD
                     KYPIVSIEDGMAEEDWDGWAELTHQIGDRVQLVGDDLFVTNVERLSRGIEAGVANSIL
                     VKVNQIGSLTETLESVELAHRSGYTAVMSHRSGETEDTTISDLAVATNCGQIKTGAPA
                     RSDRVAKYNQLLRIEELLGADAAFRGSSALARGAATS"
     gene            2346..3311
                     /gene="speB"
                     /locus_tag="PLP95_00385"
     CDS             2346..3311
                     /gene="speB"
                     /locus_tag="PLP95_00385"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: protein motif:HMM:NF002564.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008783 - agmatinase activity [Evidence
                     IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HPB44292.1"
                     /translation="MESDPTKPESGDVSDAAGFDMTWGGQLSFGRRPATRNLDGVDLA
                     IVGVPFDNAVTNRPGARFGPRAIREASTLSSEFPDGLWPWTWNVFERHRTIDYFDIAY
                     HPGIPSEMAQNVRDHIGRLLDAGVNVVALGGDHVISYPLLQATSQRYGTLSLIHFDAH
                     SDTWDAGEELNHGTMFLRAAREGLIDPTTSVQVGLRTPNDTHGFNVIGADRFFELGVD
                     ATIAEIRAIVGARPCYLTVDIDFLDPAYAPGTGTPVVGGATTWQARQLLMGLAGLDIV
                     GADLVEVAPAYDPTGQITALAGATLAHDELYLLGIARDRRAGIIR"
     gene            complement(3369..4775)
                     /locus_tag="PLP95_00390"
     CDS             complement(3369..4775)
                     /locus_tag="PLP95_00390"
                     /inference="COORDINATES: protein motif:HMM:TIGR00444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG family protein"
                     /protein_id="HPB44293.1"
                     /translation="MSPRVIVVGLGPGGADLVTQGALDLIGASEAVFVRTQRHPACAI
                     FADEAAPGRRQVTYFDDVYDTAPTMDDVYREIVDHLCAAAVEHGSVVYAVPGSPVVAE
                     RTVQLLVADDRVETRIVAALSFLDLAWARLGIDPIDRGVRVVDGHRFATDAAGQTGPL
                     LVCQCDSAMILSDIKLSLEDWPAEPVTVIQRLGLPDEAIFEVPWQELDREVVPDHLTS
                     LYIPTLDAPVAAELVAFDELVHALRTGCPWDREQTHASLKRYLLEESYELLEAIDDLD
                     PVTGDGADELCEELGDVLFQVFFHATIAAEAGWFTLADVARDVHDKLYHRHPHVFGDA
                     VVSSTEEMVSQWEATKVVEKQRESMLDGIPRELPALATALKMAKKAAAKGFVDDGPGP
                     QVRALAASIETAGLGVEDLGDLLFAIAGSAQASGIDPEDALRLSSRRFRERFIEAEQA
                     EQAEHTTVASETSGPQRS"
     gene            complement(4772..5752)
                     /locus_tag="PLP95_00395"
     CDS             complement(4772..5752)
                     /locus_tag="PLP95_00395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB44294.1"
                     /translation="MKRLLATTTAAALSLAMFATGCGVAADETAATVDGTDISTRTIG
                     ELAESSEILKNLQIAATSRDGVVNAEAARAALGQVVQVRAIEAFVERFGDGVTEADRA
                     AAEAISTGATDEAFASLTTDYLAANNALTRLSDSLADMAPEEARSELFRWTHRSIGAT
                     HCIEAALVDGAAAEQFGAAVGTDAPLADIDAEAIEFTPLTDGEECHAEAVLTGFVTPI
                     RNAPIGSWYSEEITVQGMTYTMFVKKGSEEATSLDNPATVELVDGIMSSGQTTQFLQF
                     AAVSQARVAIDPRYGTWNVDLGAVDSPLRPTFPAASSSNEPSLEDLLGAA"
     gene            complement(5773..9321)
                     /gene="mfd"
                     /locus_tag="PLP95_00400"
     CDS             complement(5773..9321)
                     /gene="mfd"
                     /locus_tag="PLP95_00400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HPB44295.1"
                     /translation="MTVTDPPRLEAGRSAFDGAGALGALAPLLRDEPALTSTFGRVSA
                     TMAVPEPARAATIAAIATLSERAPIVVATPTAQDCERLASDLVSFLGHDAVETFPAWE
                     TLPFERVSPSVETMGRRLRTMWRLRTPGRSPRVVVASARALVQRLGPHADDVEPVVVR
                     PGAVIDQERLVGGLVQAGYRREYQVEHRGELAVRGSIVDVWPSTADGPIRIDLWGDEV
                     DRLTSFSVNDQRSDTDVDQVEIFPCRELMLTDEVRERAAALETSEPWGAETWARLAGG
                     ETFDGMESWLPWLVAGGASGDGERVLFDLIGDDAQVVLVEPRRMRDRAGDIIAEEVDL
                     ARVLSGTWGASSDGVLPRLHLDFDRLLSHTRAPSWSMTVAAEGPEVETIQAAGWASAS
                     GDGSRLVAQLKDLASTGYRIVVAAEGAGSAERLARLLTEQGVSYPLHLEGGPALCEPG
                     GHIVVAPLHRGFILTTSKVAVLSEADLTGRRRTHRVVRARKRQSESYFDDLKVGSYVV
                     HHHHGVARFGGMVKRSIGGVERDYLLLEYRGDDKLYVPSEQIDSVRHYSGGETPTLSK
                     MGGADFAKAKSKVASEVATIAQELVVLYQKRVHTPGIAFAADTPWQHEMEDAFAFAET
                     PDQLAAIADVKGDMEADRPMDRLVIGDVGFGKTEVAIRAAFKAVQDGYQVAVLVPTTL
                     LAQQHFATFSERFAAYPVRVEVMSRFLTAKQGKEVAAGLAAGEVDIVIGTHRLLSGDV
                     EFKRLGLLVVDEEQRFGVSHKEAIKQFRTDVDVLTLTATPIPRTMEMSLTGIRDLSLL
                     QTPPAQRQPILTYVGEYDDRAVAESIRRELLREGQVFFVHNRVADIDQKAEELRDLVP
                     EARIAVAHGQMDEGTLERVVVEFWEGAYDVLVCTTIIESGIDMPTVNTLVVERADLLG
                     LGQLHQLRGRVGRSGSRAYAYLFFPPDRELSEEAYERLRTIGEATELGSGFRIAMRDL
                     EIRGAGNLLGTGQSGHIAAVGYDLYCSMVNEAINELNGETPTEPAEIKIELPVDANLP
                     ASYVARDDLRLEAYRRLAVVTTDADVDDIASEWTDRFGPIPEPAQSLLDVARVRVQCV
                     RTGVTEISVAKSTGFGGPALVAKLAPLELPQSKQMRLERLYKGAHYKSDLRQVQLPVK
                     SGRVVIETLLDAFAKLVPIDDVVPASEVTSSGKRGI"
     gene            complement(9318..9950)
                     /gene="pth"
                     /locus_tag="PLP95_00405"
     CDS             complement(9318..9950)
                     /gene="pth"
                     /locus_tag="PLP95_00405"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HPB44296.1"
                     /translation="MVLRRGGSARSGAKEHTTTPWDLMVVGLGNPGPKYRGTLHNVGV
                     EAIELIAERTGSRFRTAKESALVAETRLGPARVALVFPQTYMNESGRAVAPLVKRYGI
                     TDLSTLVVVHDELDLEPGRMKVKFGGGLAGHNGLRSIKAHLHSDGFTRIRIGVGKPPG
                     GKDRGVDHVLAQPTKRDRELISVCVHEAADAVEMILTDGVEAAMGRYNQK"
     gene            complement(9986..10597)
                     /locus_tag="PLP95_00410"
     CDS             complement(9986..10597)
                     /locus_tag="PLP95_00410"
                     /inference="COORDINATES: protein motif:HMM:TIGR00731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HPB44297.1"
                     /translation="MAEVTLTATTGRELGTSSSRRLRAEGFIPATVYGMGKDASSVQV
                     ARPDFRKAMTTDAGVNALIKLTVDGETEYALVKEIQRHTVRREVIHIDLLRIDPEVPI
                     VLDVPVVLTGEAKQVTAGGGMINQRLRSLRVTVRPDSIPTEITMSITNLDVEQTFTVG
                     DLALPDGVTTELDPTTAVVSAPLTRAAIVAARQAAKGDDAAES"
     gene            complement(10744..11730)
                     /locus_tag="PLP95_00415"
     CDS             complement(10744..11730)
                     /locus_tag="PLP95_00415"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0044249 - cellular biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HPB44298.1"
                     /translation="MELVTKKRLHLVAGRNNRPLAEEVAQRLDVSLGPISVSEFANGE
                     LHCKYGDSIRGSDVFIFQGHSSTPELSINDALMEHLIMVDAARRASAKRITVVAPLYG
                     YSRQDRKAEGREPITAKLIADLFKVAGAKRLVSVDLHSGQIQGFFDGPVDHLTAMPVL
                     VQWMQDNLGDTDLVVASPDAGRVKVSERYANQLHANIAMVHKRRVRGLKNTVEAKDVV
                     GDVAGKVCVLIDDMIDTGGTLVAAAELLAERGASKVYAAATHGVFSGPAIERIENSVL
                     EKVVITNTVYQPPQNRIAKIEVLSAAGIIADAIDAVFEDTSVSEIFHGENQH"
     gene            complement(11744..12856)
                     /locus_tag="PLP95_00420"
     CDS             complement(11744..12856)
                     /locus_tag="PLP95_00420"
                     /inference="COORDINATES: protein motif:HMM:NF013308.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTP transferase domain-containing protein"
                     /protein_id="HPB44299.1"
                     /translation="MTTTVEQRRPLYAVVLAAGEGSRMKSERPKPLHRLCGKPMLAYV
                     LESLETSSVDAIAIVVGHKAEVVTKKITEHPLSTALHFAHQEVQRGTGDAAQIGLAGL
                     PEDVDDDSDVIVLPGDAPLLRAETMRELVERHRSSGVAATLLTAELDDPTGYGRIVRA
                     AGGAVVDIVEQKDATPDQLAIREVNTSIYCFKRSLLTPALRRLSPDNAQGEYYLTDVV
                     KVLASAGHSVDSMIAPNPEEASGVNDRLQLSHAEAEMRRRTNEALMRSGVSMLDPATT
                     YVETTVNVGRDVTLFPGVVLQGRTVIGDGAEIGPNSRLVDSVVGRDAIIESTVARGAV
                     IGDRAVVGPFAWLGAGSEIPSDAVTGASYNSDTAQP"
     gene            complement(12906..12978)
                     /locus_tag="PLP95_00425"
     tRNA            complement(12906..12978)
                     /locus_tag="PLP95_00425"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(12943..12945),aa:Gln,seq:ttg)
     gene            complement(13060..>14038)
                     /locus_tag="PLP95_00430"
     CDS             complement(13060..>14038)
                     /locus_tag="PLP95_00430"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HPB44300.1"
                     /translation="TRGDGPFCVVIPAYNEERSISTCLDAVLAQTWDNLEVLVLDGGS
                     QDRTREIVAEYTAVDPRVRVVANPGRTQPAALNAARASITSDWMIRMDAHSTIPANYV
                     ESVMAHLGTGKWGGVGGRKDGVATTAAGRAIVAALGSKFGVGNSKYHHSTEVETVDHI
                     PFGAYPMSVVNELGGWDESNVSNEDFEFDYRVRQSGRELLFDPGIVIFWQTRETIGSF
                     FEQYRRYGRAKAVTLLTHPKSASPRHVLPAVLALAMMFAAVIGWRKPKVAAAIVTPYL
                     GGIAVATASIAPKLTAPESLRFVPGALMAMHLGYGVGLLEAFVLGRRNR"
BASE COUNT         2469 a         4882 c         4335 g         2352 t
ORIGIN      
        1 gcatcgtgat gaagatcggc acattccacg ggatgatgcc ggcgcacaca cccaccggct
       61 cccgacgcac gagcaccggc cccaacatcc cgtcgcggac ctgttcgaac tcgtagctct
      121 tgacgaggtt gcggaagaag tcgagcgtca ttcccgacgc tccgacctgg ccgaagtggc
      181 tgaacaacaa cggtgagccg acctcggcgg tgatcagttc ggccaactcg ttggagtgct
      241 cgatgaacag gtcatagaag cggtccatga tcgccccacg ttcggccgca ctcatgcgcg
      301 gccacgggcc gttgtcgaac gcgtcacgcg ccgcggcaat ggccgcgtcg acatcggcct
      361 cgaccgcctc tggagcactc gccagcagct cgccggtcga cgggcttcgc agctcgaaac
      421 gccgatcgct cgcgggggcg acccacttgc cgccgatgaa gagcgagtcg aagctcggga
      481 catcaaccat tacaaacccc cggtccggcc gtcgcggcca ccttctgacg tcccatcaga
      541 ttaccacggg acccaccgct cctgaacggc cgttcaacca gccgctgcct cgcggagcgc
      601 ctcgtccacc cgggtgaacg gccagacctc tggcaggtgc aactcgatcg gaccggactc
      661 cggcacggag tacgactcgt cgcccggata gacccgcccg aagcgctcgc gcgcctccat
      721 ttcgatgagc gaatccgacg acagcgcgga gacccgctcg gagagtcgcc gattctccga
      781 tttcacctcg acgagttgct gctcggccac cgccatgtca cgactctggg ccatcaggcg
      841 tcgcgtcggc gagatcagca cggagaccgc gatgaccatc gccaccacga ccccgactcg
      901 aacgacacga cgcctgcctg tcacgacgtt gccgccccgc gggcgagcgc cgaactgcct
      961 cggaacgccg catccgcgcc gagcaactcc tcgatacgca gcagctggtt gtacttcgcg
     1021 acccgatccg aacgagccgg agctccggtc ttgatctggc cgcagttggt cgcgacggcg
     1081 agatcggaaa tcgtcgtgtc ctcggtctca cccgagcgat gcgacatcac cgcggtgtag
     1141 ccgctgcgat gtgccagctc caccgactcg agcgtttcgg tgagcgaacc gatctggttg
     1201 accttcacca ggatcgagtt ggcgaccccg gcttcaatgc ctcgggacag gcgctcgacg
     1261 ttggtgacga acagatcgtc gccgacgagc tgcactcggt cgccgatctg gtgggtcagc
     1321 tccgcccaac cgtcccagtc ctcttccgcc atgccgtctt cgatcgacac gatcgggtac
     1381 ttgtcggtga gctcggcgag gtagcccgcg aactccgagg ggttcagcgt tcggccttcg
     1441 cccgccaaca cgtagttgcc gtcgcggtag aactccgaac tcgccgcatc cagtgcgatc
     1501 gcgatctgct cacccggctc gtaacccgcc ttttcgatcg cagcgatgag caactgcacc
     1561 gcctcctcgt tgctcgcgag gttcggcgcg aatccaccct catcgccgat cgcggtgctg
     1621 agtcccttgt cgtgaagcac gttcttgagc acgtggtagg tctcggtccc ccatcgaagc
     1681 gcctcggaaa aggatccggc gcccaccggc atgatcatga attcctgcag gtcgacgttg
     1741 ttgtcggcgt gttcgccacc gttgagcacg ttcatcatcg gcaccggcaa cacgtgggca
     1801 ttcgcgccgc ccacgtagcg aaacaccggc aaaccgacct cttcggccgc agcgcgggcg
     1861 agcgcaagcg aaacaccgag gatggcgttg gcgccgagtc ggcccttgtt gtgggttccg
     1921 tcgacgtcca acaggatccg gtcgatctgg cgctgatcga gcgcatcgag ccccacgatc
     1981 gcttcggcga tgtcgtcgtt gacgtgctcc accgccttgg tgacggcctt gccgagatag
     2041 tccgagccac cgtcgcgaag ctccaccgct tcgaactgcc ccgtggaggc gccggagggt
     2101 accgcggcgc ggcctgcggc tccggtggtc aggaggactt ccacctcgac ggtcgggttg
     2161 ccacgggagt caagaatctg acgaccgtgg atgtgctcga tgatgctcat atggctactc
     2221 cacaaacggt tcgaatcaac gatagttcga ggttcatggg agcacaggga gtccccgccc
     2281 gccaaaccga ttcgccggtc cggcaccacc accgctcacg ccgatccagc tgcaaagctg
     2341 gccccatgga atcggacccc accaagcccg agtcgggcga tgtctccgac gccgcaggat
     2401 tcgacatgac ctggggaggc cagctgtcct tcggccgacg gcctgcgacc cggaacctcg
     2461 acggggtcga cctcgcgatc gtcggtgttc cgttcgacaa cgccgtgacg aatcgccccg
     2521 gcgcccgctt tggcccccgc gccattcgcg aggcgtccac gctctccagc gagttccctg
     2581 acggactgtg gccgtggaca tggaacgtgt tcgagcgtca ccgcacgatc gactacttcg
     2641 acatcgccta tcaccccggc attccctccg aaatggcgca gaacgtgcgc gaccacatcg
     2701 gccgcctgct cgacgccggg gtcaacgtcg tcgcactcgg cggtgatcac gtcatctcct
     2761 accctctgtt gcaggcgacc tcgcagcgct atggaacgct gtcactcatc cacttcgatg
     2821 cccacagcga cacctgggat gcgggcgagg aactgaacca cggcacgatg tttctgcggg
     2881 ccgcccgcga gggactgatc gacccgacga catcggtgca agtcggactg cgcaccccga
     2941 acgacacaca cgggttcaac gtcatcggtg ccgatcgctt cttcgaactc ggcgtcgatg
     3001 cgacgatcgc ggagatccgc gcaatcgtcg gggctcgacc ctgctatctc accgtcgaca
     3061 tcgacttcct cgatcccgcg tacgccccgg gcactggcac cccggtcgtc ggtggtgcaa
     3121 cgacgtggca ggcacgacag ctcctcatgg ggctcgctgg actcgacatc gtcggcgccg
     3181 acctggtcga ggtggcaccg gcctacgacc cgaccgggca aatcacggcg ctcgcggggg
     3241 caacccttgc gcacgacgag ctctacctcc tcggcatcgc ccgcgaccga cgcgccggaa
     3301 tcattcgctg agccgcatcg atacagcagc cgccgcgata cagcggccgc ctcgaatcag
     3361 cggtcgactc aggaccgctg cggccccgac gtctccgacg ccacagtcgt gtgttcggcc
     3421 tgttcggcct gttcggcctc gatgaagcgt tcccggaacc gacgactgct caggcgcaag
     3481 gcatcctccg ggtcgatccc cgaggcctgc gccgacccgg cgatcgcaaa cagcagatcg
     3541 ccaagatcct cgaccccgag ccctgcggtc tcgatcgagg cggcaagcgc tcgaacctgc
     3601 gggccaggac cgtcatcgac gaaccccttc gccgcggcct tcttcgccat cttcagcgcc
     3661 gtggcgagcg cagggagctc gcgcggaatt ccgtccagca tcgactcgcg ctgcttctcg
     3721 accaccttcg tcgcctccca ctgcgacacc atctcttcgg tcgaggagac gaccgcatcg
     3781 ccgaagacgt ggggatggcg gtggtagagc ttgtcgtgca cgtcgcgggc aacgtccgca
     3841 agcgtgaacc accccgcttc ggcggcgatc gtcgcgtgga agaacacctg aaacaacacg
     3901 tccccgagtt cctcgcacaa ctcgtcggcg ccgtctccgg tcacggggtc gaggtcgtcg
     3961 atcgcctcca acagctcata gctttcctcc aggaggtaac gcttcagcga ggcgtgggtc
     4021 tgctcgcgat cccacggaca accggtgcga agcgcgtgca ccagctcgtc gaaggcgacc
     4081 aactcggccg ccaccggcgc gtcgagggtc gggatgtaca gcgacgtcaa gtgatcgggc
     4141 acgacctcgc ggtcgagttc ctgccagggc acctcgaaga tcgcctcgtc gggcaaaccg
     4201 agtcgctgga tgaccgtcac cggctcggcg ggccaatcct cgagcgagag cttgatgtcg
     4261 ctcaggatca tcgccgagtc gcattggcac acgagcaacg ggcccgtctg gcccgcggcg
     4321 tcggtcgcaa atcggtggcc gtcgacgacc cgaaccccgc gatcgatcgg atcgatcccg
     4381 agtcgcgccc acgccaggtc gagaaacgac aacgccgcca cgatcctcgt ctccacccga
     4441 tcatcggcca ccagcaactg cacggtccgt tcagccacga ccggagaccc aggcaccgca
     4501 tagaccaccg agccgtgctc aacggcggct gcgcacagat gatcgacgat ctcgcgatac
     4561 acgtcgtcca tcgtcggcgc tgtgtcatag acgtcgtcga agtaggtcac ctgacgtcgc
     4621 cccggtgcgg cctcgtcggc gaaaatcgcg cacgcgggat gtcgctgcgt ccgaacgaag
     4681 accgcttcgc tggccccgat caggtcgagg gcgccttgtg tgacgagatc cgcgccgccc
     4741 ggaccgagcc cgacgacgat gacccgcggg ctcacgctgc tccgagcagg tcctcaaggg
     4801 agggttcgtt gctcgaggaa gccgccggaa acgtcggccg caacggtgag tcgacggctc
     4861 ccaggtcgac gttccacgtt ccgtatcgcg gatcgatggc cacacgggcc tggctgacgg
     4921 cggcgaactg caggaactgg gtggtctgcc ccgacgacat gatcccgtcg accaactcga
     4981 cggtggcggg gttgtcgagc gaggtggcct cctcggaccc cttcttgacg aacatcgtgt
     5041 aggtcatccc ctgcacggtg atctcctcgg agtaccacga accgatcggg gcgttgcgaa
     5101 tgggcgtcac aaacccggtg agcaccgcct cagcgtggca ctcctcacca tcggtgagcg
     5161 gggtgaactc gatcgcttcc gcgtcgatat ctgcgagcgg cgcgtcggtg ccgaccgcgg
     5221 cgccgaactg ctcggccgcc gcgccgtcga cgagcgcggc ctcgatgcag tgggtcgcac
     5281 cgatcgaacg gtgtgtccaa cggaagagct ccgaccgggc ctcttcgggg gccatgtcgg
     5341 cgagcgaatc cgacaacctg gtcaacgcgt tgttcgccgc gagatagtcc gtggtgagcg
     5401 aggcgaaggc ctcgtcggtc gcgccggtgg agatcgcttc cgccgcggcc cgatcggctt
     5461 cggtcacccc gtcgccaaag cgttcgacaa acgcctcgat cgctcgaacc tggaccacct
     5521 gccccaacgc agcgcgcgcc gcttcggcgt tgacgacacc gtcgcgcgac gtagcggcaa
     5581 tctgcagatt cttgagaatc tcgctcgact cggcgagttc gccgatcgtg cgggtcgaga
     5641 tgtcggtgcc gtcgacggtg gccgccgtct cgtctgcggc aaccccgcag cccgtggcga
     5701 acatggcaag cgacaacgcg gctgcggtgg tggtggcgag aagtcgtttc acagaacctc
     5761 agatcgtact ggtcagatgc cccgtttgcc cgaggaggtc acctcggagg cgggaacaac
     5821 gtcatcgatg gggacgagtt tcgcaaaagc gtccaacaag gtctcaatca cgactcgacc
     5881 cgacttcaca ggaagctgga cttggcgcag atcggactta tagtgcgccc ccttgtacag
     5941 gcgctcgaga cgcatctgtt tcgattgcgg caactcgagc ggagcgagct tggcgacgag
     6001 cgcgggaccg ccgaacccgg tcgacttggc gaccgagatc tcggtgactc cggtgcgaac
     6061 gcactgcacg cgaacccgag ctacgtccaa caacgactgg gccggctctg ggatcggccc
     6121 aaagcggtcg gtccactcgc tcgcaatgtc gtcgacatcg gcgtcggtcg tcaccaccgc
     6181 cagccggcga tacgcctcga ggcgaaggtc gtcacgggcg acataggacg ccggcaagtt
     6241 ggcgtccacc ggcaactcga tcttgatctc ggccggttcg gtcggcgtct caccgttcaa
     6301 ctcgttgatc gcctcgttga ccatcgagca gtacaggtca tagcccacgg cggcgatatg
     6361 gccgctctgg cccgttccca acagatttcc agcgccgcgg atctcgaggt cgcgcatggc
     6421 gatccggaac cccgacccga gctcggtggc ctcgccgatc gtgcgcaagc gttcgtaggc
     6481 ctcctccgac agctcacgat cgggcgggaa gaacagatag gcgtacgctc ggctgccgga
     6541 gcgcccgacg cgtccgcgca actgatgcaa ctggccgagc ccgagcagat ctgcacgctc
     6601 gacgaccagc gtgttcaccg tcggcatgtc gatgcccgac tcgatgatcg tcgtacacac
     6661 gagcacgtcg tacgcgcctt cccagaactc gaccaccaca cgttcgagcg tgccctcatc
     6721 catctgtccg tgagcgacgg cgatgcgagc ctcgggcacc aggtcgcgca gctcctcggc
     6781 cttctggtcg atgtcggcca cccgattgtg cacgaagaac acctgcccct cgcgcagcag
     6841 ctcacggcgg atcgactcgg ccaccgcccg gtcgtcgtac tcgccgacat aggtgaggat
     6901 cggctgacgt tgagccggcg gcgtctgcaa cagcgacagg tcccgaattc cggtgagcga
     6961 catctccatc gttcgaggga tgggggtcgc ggtcaacgtc agcacatcga cgtcggtgcg
     7021 gaactgcttg atcgcctcct tgtggctgac cccgaagcgc tgttcctcat cgaccaccaa
     7081 caggccgagc cgtttgaact cgacgtcgcc cgacaacagg cggtgagtcc cgatcacgat
     7141 gtcgacctcg cccgcggcca gcccggcggc gacctctttg ccctgcttcg cggtgaggaa
     7201 acggctcatc acctcaacgc gaaccggata ggcggcaaag cgttccgaga acgtcgcaaa
     7261 gtgctgttgc gcgagcagtg tcgtgggcac gagcaccgcc acctggtatc cgtcctgcac
     7321 cgccttgaac gcggcgcgga tcgcgacctc cgtcttgccg aaccccacat caccgatgac
     7381 cagtcgatcc atgggccgat cggcttccat gtcacccttg acgtcggcga tggccgctag
     7441 ctgatcgggg gtttcggcaa acgcgaacgc gtcttccatc tcgtgttgcc acggagtgtc
     7501 ggccgcgaac gcgattcctg gcgtatgcac ccgcttctgg tacagcacca ccaactcctg
     7561 ggcgatggtc gccacctctg acgcgacctt ggacttggcc ttggcgaaat cggcgccccc
     7621 catcttcgac agggtcggcg tctcgccgcc ggagtagtgc cgcaccgagt cgatctgctc
     7681 ggagggcacg tacaacttgt cgtcgccgcg gtattccaac agcaggtagt cccgctcgac
     7741 cccgccgatg cttcgcttca ccatgccgcc gaagcgagca accccatggt ggtggtgtac
     7801 gacgtagctg cccaccttga ggtcgtcgaa gtagctctcc gactgacgct tgcgggcgcg
     7861 cacgacccgg tgagtgcgac gacgacccgt caggtctgct tcactcagca ccgcgacctt
     7921 cgaggtcgtg agaatgaatc cgcggtgaag cggcgccacg acgatgtgac cgcccggctc
     7981 gcacagcgcc gggccgcctt caaggtgcag cgggtaggac acaccctgtt cggtgagcag
     8041 ccgggcaagc cgctcggccg atcctgcacc ctccgccgcg acgacgatgc gataccccgt
     8101 gctggcgaga tccttcaatt gtgcaaccaa gcgcgaaccg tcgccggagg ccgacgccca
     8161 cccggccgcc tggatcgtct cgacctcggg cccttcggca gcgaccgtca tcgaccaact
     8221 cggcgcccgt gtgtggctca gcaaccgatc gaaatcgaga tgcagccgcg gcagcacccc
     8281 gtccgaactc gccccccacg ttcccgacaa cacccgcgcc aggtcgacct cctcagcgat
     8341 gatgtcaccg gcccgatcgc gcatccgtcg cggttcgacg aggaccacct gcgcgtcgtc
     8401 gccgatgaga tcgaacagca cccgttcccc gtcgccagac gccccgccgg ccaccaacca
     8461 cggaagccac gactccatgc cgtcgaacgt ttctccgccg gcaaggcgcg cccacgtttc
     8521 ggcaccccac ggctcggagg tctcgagcgc tgccgcacgc tcgcgcacct cgtcggtgag
     8581 catcagctcg cgacacggga agatctcaac ctggtcgaca tcggtatcgg atcgctgatc
     8641 gttgaccgaa aacgaggtga gccgatcgac ctcatcgccc cacagatcga ttcgaatcgg
     8701 accgtccgcc gtcgatggcc acacgtcgac gatcgaaccg cgcaccgcca gttcgccgcg
     8761 gtgttccacc tggtattcac ggcgatagcc ggcctggact aggccgccca cgagccgttc
     8821 ctggtcgatc accgcgccgg gtctgaccac gaccggttcg acgtcatccg catgcgggcc
     8881 gaggcgctgc acgagcgccc gcgctgaggc cacgacgacc ctcggcgacc gtccgggtgt
     8941 ccgcagacgc cacatcgtgc gcagacgtcg ccccatcgtt tcgaccgacg ggctgacgcg
     9001 ctcgaacggg agggtctccc aagccggaaa ggtctcgacc gcgtcgtgtc caaggaagct
     9061 caccagatcg ctcgcgaggc gctcacaatc ctgcgcggtc ggcgttgcca cgacgatcgg
     9121 cgcacgctcc gacaacgtgg cgatcgctgc gatcgtcgcg gcccgagcgg gttcgggcac
     9181 cgccatggtc gccgagaccc gaccaaaggt cgacgtgagc gccggctcat cccgcaagag
     9241 cggagcgagt gcgcccaacg cccctgcacc gtcgaatgcg ctgcggcccg cttcgagcct
     9301 cggtgggtcg gtcacggtca tttctggtta taccgaccca tcgcagcctc caccccatcg
     9361 gtcaggatca tctcgaccgc gtccgccgcc tcgtgcacgc acaccgaaat cagttcgcgg
     9421 tcgcgtttcg tcggctgggc cagcacgtga tccacccctc ggtccttgcc acccgggggc
     9481 ttaccgaccc cgatgcggat gcgcgtgaac ccgtcggaat gcaggtgggc cttgatggaa
     9541 cgaagaccgt tgtggccagc gagcccgccg ccgaatttca ccttcattcg gcccggctcg
     9601 aggtcgagct cgtcgtgcac gaccaccagc gttgacaggt cggtgattcc gtaacgcttc
     9661 accagcgggg ccacggctcg gcccgactcg ttcatgtagg tctgcggaaa gaccagcgcc
     9721 acccgcgccg gaccaagccg ggtctccgcg accagcgccg attccttggc ggtccgaaac
     9781 cgcgagccgg ttcgctcggc gatcaactcg atcgcctcga ctccgacatt gtgcagggtc
     9841 ccgcggtatt tcggccccgg attgccgagg ccgaccacca tcagatccca cggggtcgtg
     9901 gtgtgctcct tcgcaccgga cctggcggac ccgccgcgcc gcaacaccac gatcgatcag
     9961 cagggccggg gcaaaccgga tgcgatcagg attccgcggc gtcgtcgccc ttggccgcct
    10021 ggcgggcagc gacgattgca gcgcgggtca gcggggccga cacgaccgcc gtcgtcgggt
    10081 ccaactcggt ggtcaccccg tccggcaacg ccaggtcgcc gacggtgaag gtctgttcga
    10141 catcgaggtt ggtgatcgac atcgtgatct cggtggggat cgagtccggc cgcaccgtca
    10201 cccggaggct acgcagacgc tggttgatca tgccgccacc tgcggtgacc tgcttcgcct
    10261 cgccggtcaa cacgacgggg acgtcaagaa cgatcggaac ctcgggatcg atgcgcagga
    10321 ggtcgatatg gatcacctca cgacgaaccg tgtgacgctg aatctccttg acgagcgcgt
    10381 actcggtttc accgtcgacc gtgagcttga tgagcgcgtt gacaccggcg tcggtcgtca
    10441 tcgccttgcg gaagtcgggg cgagcgacct gcaccgagct cgcgtctttg cccatgccgt
    10501 acaccgtcgc gggaatgaag ccttcggcgc gcaggcggcg cgacgagctg gtgccgagtt
    10561 cgcggccggt ggtggcggtc agggtgacct cagccataac aattactcct caaacgaacg
    10621 tcacggccgc gcggggacgc gccaaggccg gattgagaga ctatccgccc accccggcga
    10681 gggccaagtg gggcccgaaa tcgtgccgga gcgcggcggt ttgcgtcagc gctgacgttt
    10741 ggatcagtgc tggttctcgc cgtggaagat ctccgacacc gacgtgtctt cgaacaccgc
    10801 atcgatcgcg tcggcgatga tgccggcggc cgacaggacc tcgatcttcg cgatccggtt
    10861 ctggggcggc tggtagacgg tgttggtgat cacgaccttc tcgagcacgg aattctcgat
    10921 ccgttcgatc gccggaccgc tgaagactcc gtgggtcgca gccgcgtaca ccttggacgc
    10981 cccgcgttcg gccagcagtt cggcggcggc caccagcgtc ccgcccgtgt cgatcatgtc
    11041 gtcgatcagc acgcacacct tgccggcgac gtcaccgacg acatctttgg cttcgacggt
    11101 gttcttcaga ccgcgcacgc gacgcttgtg aaccatggcg atgttcgcgt gcaactggtt
    11161 cgcgtagcgc tcggagacct tcacccggcc ggcgtcgggg gatgccacga ccagatcggt
    11221 atcgccgagg ttgtcctgca tccactgcac cagaaccggc atcgccgtga gatgatcgac
    11281 cgggccgtcg aagaacccct ggatctgccc tgaatgcagg tcgactgaca ccagccgctt
    11341 cgcacccgcc accttgaaca gatcggcgat gagtttcgcc gtgatcggct cgcgtccctc
    11401 tgcctttcgg tcctgacgcg aatacccgta gagcggagcg acgaccgtga tccgcttggc
    11461 cgaggcgcgg cgggcggcgt cgaccatgat gaggtgctcc atcaacgcgt cgttgatgga
    11521 cagttcgggt gtcgaggaat gcccctggaa gatgaacacg tcggagccgc ggatcgaatc
    11581 gccgtacttg cagtggagct caccgttggc gaactccgac accgagatcg gtccgagcga
    11641 gacatcgagg cgttgcgcca cctcctctgc gagcgggcga ttgttacgcc ccgcgacgag
    11701 gtgcaaacgc ttcttggtga cgagctccat gagcaactcc cgatcagggc tgggcggtgt
    11761 cagaattgta ggacgctccg gtgaccgcgt cggacgggat ctcggaccca gcgcccaacc
    11821 aggcgaatgg accgaccacg gcgcggtcgc cgatcaccgc tcctcgagcg accgtcgact
    11881 cgatgatcgc gtcgcgtccg accacgctgt cgacgaggcg cgagttcgga ccgatctcgg
    11941 cgccatcgcc gatcaccgtt cgcccctgga ggacgacacc gggaaacagc gtcacgtcac
    12001 ggccgacgtt cacggtcgtt tcgacatagg tcgtcgccgg atcgagcatc gaaacacccg
    12061 accgcatcaa cgcctcgttc gtgcggcgac gcatctccgc ctccgcatgg gacagctgca
    12121 accggtcatt gacccccgaa gcctcctccg ggttgggcgc gatcatcgag tcgacgctat
    12181 ggcccgcact ggcaagcacc ttcacgacat cggtcaggta gtactcgccc tgcgcgttgt
    12241 ccgggctgag tcgccgcagg gccggtgtga ggagcgaacg tttgaagcaa tagatcgacg
    12301 tgttgacctc gcggatggcg agttgatcgg gggtcgcatc cttttgttcg acgatgtcga
    12361 ccaccgcccc gcccgcggcc cgcacgatcc ggccgtaccc ggtcggatcg tcgagctccg
    12421 cggtcagcag cgtcgccgcc acaccgctgc tgcgatgccg ctcgacgagc tcgcgcatcg
    12481 tttctgcgcg caatagcggc gcatcgccgg gcaggacgat cacgtcgctg tcgtcatcga
    12541 catcctccgg cagaccggca aggccaatct gagctgcgtc tccggtgccg cgctgtacct
    12601 cttggtgagc gaaatgcagt gcggtcgaca gcggatgttc ggtgatcttc tttgtcacca
    12661 cctcggcctt gtgacccacg acgatggcga tcgcatcgac cgaactcgtc tcgagggact
    12721 ccaggacgta ggccagcatc ggtttcccgc acaaccggtg caacggtttg ggtcgttcgg
    12781 atttcatccg cgacccttcg ccagccgcga gcaccacggc gtagagcgga cgtcgctgct
    12841 cgactgttgt ggtcacggcg cccccggcac cgtccgcatc atccgtgcgg acgaatcgat
    12901 gaatgctcgc ggggcaggac tcgaacctgc aacaaccgga ttcaaagtcc ggtgttctgc
    12961 caattgaact acccgcgaag gatctgttga gcgtattcga tcgaacccgt cattccacaa
    13021 ccgaaaccac cgtggcccgc cgcgaatccg aaccgcgcct cagcggttac gccgacccag
    13081 cacgaacgct tccaacaatc ccacgccgta ccccaaatgc atcgccatca acgcacccgg
    13141 aacgaatcga agcgactccg gggcggtcaa cttaggtgcg atcgacgcgg tcgccaccgc
    13201 gatgccgccg agatagggcg tgacgatcgc agccgcgacc ttgggcttgc gccagccgat
    13261 gaccgccgcg aacatcatcg ccaacgccag cacggccggc aggacgtggc gcggcgaggc
    13321 agatttcggg tgggtgagca gcgtgacagc cttggctctg ccatagcgtc gatactgctc
    13381 gaagaacgac ccgatcgtct cccgggtctg ccagaagatg acgatgcccg ggtcgaaaag
    13441 cagctcgcgt cccgactgcc gcacgcggta gtcgaactcg aaatcctcat tggacacgtt
    13501 cgactcatcc caaccaccga gttcgttgac gaccgacatc gggtaggcgc cgaacggaat
    13561 gtgatcgacg gtttccacct cggtgctgtg gtggtacttc gagttgccga ccccgaactt
    13621 ggacccgagc gcggcgacga tggcacggcc ggcggcggtg gtggcgacac cgtctttgcg
    13681 accgccgacc ccaccccact ttccggtacc gaggtgcgcc atcaccgatt cgacgtaatt
    13741 ggcgggaatc gtgctgtggg cgtccatccg gatcatccag tccgaggtga tgctcgcacg
    13801 ggcggcattc aacgcggccg gctgggtccg ccccggattc gccacgacgc gaactctcgg
    13861 gtcgacggcg gtgtactccg ccacgatctc gcgggttcgg tcctgggaac caccatcgag
    13921 caccagcacc tcaaggttgt cccacgtctg agccaacacc gcgtccagac acgtggagat
    13981 ggaccgttct tcgttgtacg cagggatcac gacgcagaac ggtccatctc cacgtgtc
//
LOCUS       DAOOMA010000007        13670 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_4913_length_13670_cov_4.815204, whole genome shotgun sequence.
ACCESSION   DAOOMA010000007 DAOOMA010000000
VERSION     DAOOMA010000007.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 13670)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13670)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13670
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4913_length_13670_cov_4.815204"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1666
                     /locus_tag="PLP95_00435"
     CDS             <1..1666
                     /locus_tag="PLP95_00435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB44301.1"
                     /translation="AGQAFPDETFTNDGRSGSDHMDHARPLIERWVEQRTRWGFSEWY
                     SAVYYQEDLEAAITLAEFSDDEDLATLGAIAADLVLYDIAGHSVGSFVAATQGRTYKK
                     DKMSGPDTDTWDLATLVFGTEVAYGSATGSVYLASAAKYRPPAVLGAIHDAASGAATE
                     PTVERARHSLALDPLAEVSDSPEAPMGLAFDDHDNLMTYWAMAAMTPWQTVIESTDEM
                     TAYNLWDSKLFAPFAPFRPIVEAADADTVRGLARSLAPQLNIGLLSEANTYTWRSGGT
                     AQRAMLSTVQDYRKGQASQQHHIWQATLSSEAIVFTTHPRVPTEAGVPWRSNSDDWTG
                     GASMPRSAQHENLNVSIYAPLYASGDNLQGSYVESTHAYFPQERFDEVTTVGNWVLGR
                     VGDNYVALYSWRPLRWVSYDPAEYDTDKMTEPFELVADGGPNNVWITELGSAGEYATF
                     EAFVAAITANEPVVTPLGDAAVFSTGFDVEWVSPSQGLVEFGWERPFTVGTPDAATTV
                     ELSEYPRIDAPWAQVAFDSTAYDIEAGGETLHLDVVARTRRSSGDL"
     gene            complement(1770..4478)
                     /locus_tag="PLP95_00440"
     CDS             complement(1770..4478)
                     /locus_tag="PLP95_00440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB44302.1"
                     /translation="MRDVLGLQGTVVNTLEALVERHAPNWQLPRLFAGHIVGADAVAD
                     LAFTLGWLHEAGVAEVAGTAVIDAIRAVLAQVDGAATHTFFSYRVAETIGRFGGFDHN
                     EALDGLDAATLDNLRLACDSTEVLALLDALPRNYLAVLSRCEIARERLGLLDDDAGLA
                     ALLAGVRTLLGENPRGFLDDSHDGDGRYDIYTADLYLFCEPFADRLGHVWRDGMIEAL
                     ALVEAVAASDASAFGWGRSTGALSQALSIELAALVTADGQRLAIAGSATQRDWYLRLA
                     AIAADGFPERWFDRDGVITAHRFGATEGYRGPHRRLQMTFDVLGKLAEAAARWARVDD
                     ALDEPTGSFNIDGATSRDLEPRDQLVRLDQHAAVWAYRDDALEFVVPFVSGPSSDYAP
                     SPRRPGLFGSPVDSALATFVPLVHRSGDGLNPQRRLAPTGTPTSIEWRQGSVSVCHEG
                     FGALHGIDDPRPCPPISATRHATYRVVDGALELTESLRFDETPEAVSVLVPSFDRPLS
                     IAVEESSTAVRIEHVEVDGMHEWASPWGPATEVAELMVEPAESIELRWSVRPVPVVTT
                     TDLDHHYIRAVYGSIDTPGTGRLRTRGWNWEDRIERTSLGDIFHLHWPEWAFGLELTG
                     EQHRRIAEAIRYEGVKVAWTQHNLTPHLDNPGLYEPIYQVWAQACDLAIHHSEWGRDL
                     ATSRYEFAAPCRHVVIPHPHFGELADPSLHSIDRDEAAKRVGLDTTPALRIGVIGAPR
                     RQKHLDVVIEAVEGCTRDDVELVIWSHSFLDELSSDPRIVGHRYDNVDRQTYDLRIAA
                     CDVIALPFIEHSMLGTGTASDLVGLATPALVSDWPYLHEYLGDAAIRAGQSVGSWRAA
                     IDGLDAATLNASRRAMSARQAALSPTAIGALTADAIVDLCGPLDRP"
     gene            4510..5286
                     /locus_tag="PLP95_00445"
     CDS             4510..5286
                     /locus_tag="PLP95_00445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB44303.1"
                     /translation="MAVPRVYIHEFIDIIGHGRAKYMYHMTANWVPVGIAERNQRCFG
                     VWGVVGSTGTWPQVVNLWEYDDWAALGHNFEVEQRGLGLQDPSLERWWRAAAEFRSGG
                     VDRILVAAPWSPSVADHERSFTAPPAGYVHEHFRVKPGHDLTLLDAIRDAGIDAYETA
                     GLRLVGAFRRAMAGDDEVIAIWAFANWQVWADFEAALDPLSTQSGDIDCESPCGLLHA
                     WFGSIAGIVRGRERILLADAPLSPLRIGRQPDESDRTTDA"
     gene            5354..6037
                     /locus_tag="PLP95_00450"
     CDS             5354..6037
                     /locus_tag="PLP95_00450"
                     /inference="COORDINATES: protein motif:HMM:NF014997.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HPB44304.1"
                     /translation="MIRHPRLHPPIPVFGHYGEKPRWRGRIHSAAFLVSIPAGLWLMS
                     VADSAAARVAVGIYWLSLAGLFGTSASYHLFAHSAGAVKWMRRADHSMIFVLIAGTYT
                     PFCLVVLPKPWGVPMLALLWTIAVAGIVMKMVRVGTDKSQASGSWLYGVMGWIAVIAL
                     PKLVQELGWSRMALLFAGGVIYSVGAVIMSTKKLDLRPATFGYHEVWHAMTVAASACH
                     FVLVAMVVR"
     gene            complement(6077..7192)
                     /locus_tag="PLP95_00455"
     CDS             complement(6077..7192)
                     /locus_tag="PLP95_00455"
                     /inference="COORDINATES: protein motif:HMM:NF014244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HPB44305.1"
                     /translation="MQRGRGPALRFRVFGTPVRIGLTFPLMVVALPLIFGGHFGRDPW
                     MLTAWLVLVTVSVLVHEAGHVAALRACGFHPGVSLNAFGGLTSTDERGRISPWQSIGV
                     SLAGPAAAILLGITINSALIPIHGRQIVWFSAASWFVNVWWSLFNLLPIVPLDGGHIT
                     SELVEMASRRRGAAIGWLIAATAAICAAAWWFAGREHPYIVFSVLVLMIVTNSTAFAF
                     TARERRQQDIRIAHERLLDGDEDRGLEPLLAVIWSEHGWLVSSEVYTTVAWALLYRQR
                     FDELAALELARVHPDHRPLIDAAVRWYQGDLTSAMGLITESLATGSTDIPDNYFEYTF
                     RRFGELERLERYAASLPPAAAAVVGERLTGRVEAALV"
     gene            complement(7253..8314)
                     /locus_tag="PLP95_00460"
     CDS             complement(7253..8314)
                     /locus_tag="PLP95_00460"
                     /inference="COORDINATES: protein motif:HMM:NF022586.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2889 domain-containing protein"
                     /protein_id="HPB44306.1"
                     /translation="MGFTVGRNGPQNPLRSTPVLAPGWTRWTFHADSSSPNGLGSAPV
                     RCEAVARSVRIDTDRQPVVEATVTLSAETDAMARTLSAISVHDSGAPNGALELSALVG
                     HSTAGGFRARLDSALRQVGGCSALAMFLLDDLPGITLVGGYAEQYEDSRTGVDAISEI
                     FASNPEAIDFQADTCAGWAEEATLISSLRNDGEMLTPVGPPAPALWTTAHTQAPQATG
                     SWHPIPPLSANTTRRVRRIDLGPDDGGERRFETHFRDSHADFGTTERVLHEYLVRGTL
                     RDGVIADLDAEARVLPWMECPSALASAQRCVGQRVGKLRSLVRHDFRGTSTCTHLNDT
                     IRSLDSIATMGGLDAPPHR"
     gene            complement(8351..9580)
                     /locus_tag="PLP95_00465"
     CDS             complement(8351..9580)
                     /locus_tag="PLP95_00465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HPB44307.1"
                     /translation="MYEWSEEHLMLRGAMRDFLAKELLPIKEELEHGDLPPYEFMRKM
                     YSTFGMDAMADATFSKRIEFERQVAAGEASEADKPVRDATSDPGFTLIPIIELCRHCP
                     GIVTAMGVSTGLTAAAISSKGTIAQKERWVPDLLTMRKVGAWAITEPNAGSDAFGSML
                     STARRDGDEYVLNGNKTFITNGPYADTIVFIAKLDEGNDPKDRKVLSFVLDSGMAGLE
                     QSKPLRKMGMHSSPTGELFLADVRVGIDRLIGETENVAKGGREGAKATFQQERSGVAA
                     MALGIIEQCLDLSIDYAKERKQFGQRIGDFQLIQQKLAFMEVARMNVQNLVFRIIELA
                     KAGKTMTFAEASAMKLYSAQQATAVALEAVQLFGGNGYMSEFQVEQLARDAKVLQIYA
                     GTDEQQTIAVARALLNS"
     gene            complement(9695..10183)
                     /locus_tag="PLP95_00470"
     CDS             complement(9695..10183)
                     /locus_tag="PLP95_00470"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015798619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004602 - glutathione peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0043295 - glutathione binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HPB44308.1"
                     /translation="MSAYDHTISALNGTPDLMSDIAGKTALFVNTASKCGLTPQYEQL
                     QGLQDRFGDEDFTVVGFPCNQFLEQEPGTADEISEFCQVNYGVTFPLSEKIDVNGESQ
                     HSLFAELERSADVSDGHTGDVRWNFEKFLVNPSGDVVARFAPTVAPDDPAVIAAIEAE
                     LA"
     gene            10253..11119
                     /locus_tag="PLP95_00475"
     CDS             10253..11119
                     /locus_tag="PLP95_00475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB44309.1"
                     /translation="MMWRHRTARPLGATNRGAAFASALAAIAIVGATGCAKSPMQAFE
                     DLQVEETPVEVVASSLLRLDGRVALGPDLTRTLLKVQLVPEGYSWADADCAVDAVIGV
                     MGAEAFAVVPAGKIRAITWDYPGSDEAALACRSSETEARRNHSVTKSDITIADPQPGP
                     DPDPAAVRELQARGFSLGAERAGLTLEERECILKPVIGDWTDADYVLLAQRSKQIRQD
                     DVVAAVDECLDDDRAAEAATTAVPSLLAANEWAQEESDRVDALKTAAGIDPDSGEPIV
                     TDPAAASPDSEK"
     gene            complement(11079..11909)
                     /locus_tag="PLP95_00480"
     CDS             complement(11079..11909)
                     /locus_tag="PLP95_00480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020379556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HPB44310.1"
                     /translation="MITDHFLVSDQVAIVTGAGRGIGAASAMAFAEAGADVVISARSP
                     EQLDEVADRITACGRRAVCVPADLNDLDAVRGLVDVALDEFGRLDIVVNNVGGSFPKP
                     LLDTSSRMLEQAFHFNVTTAFELSKAAIPAMLQQPTIGGDANDPGTHAACGSIVNISS
                     AIGRLSDRGMAAYGTAKAALSHLTRLMATECAPRIRVNGIAVGSTATSALEMVIENDD
                     LRTALESGTPLRRLGDPLDIALGVLYLCSPAGSFMTGKILEIDGGLTSPNLEMPLPDL
                     "
     gene            12059..>13670
                     /locus_tag="PLP95_00485"
     CDS             12059..>13670
                     /locus_tag="PLP95_00485"
                     /inference="COORDINATES: protein motif:HMM:NF019581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HPB44311.1"
                     /translation="MHDVVIRDATIVDGSGARSFTGDIAIEGERIVAVGVVEGTGRRE
                     IDAAGLLVTPGWVDIHTHYDGQVTWDPEVTPSGWHGVTTVVMGNCGVGFAPVDPARRD
                     FLIQLMEGVEDIPGTALAEGMSWNWETFEEYLDEVASMPRVMDVAALVPHGAVRAYVM
                     GDDAAVHDAATPEQIDQMRAIVADALAAGAVGFSTTRTMLHRAKDGELAAGTLAEVDE
                     LVGIGQALADTGAGLFSVASDMFDARSEFEWMTTVSRESGRPVTFGCLQSPVQSDNWV
                     ELLDLARSATAEGARIVPQVAGKPASVMFGFESSLHPFLHHRAYKAIAHLPFAERIAK
                     LKSPEVREAILAERVEYRGFQAILFEGFDRLYPLGDPPQYEPEPEASVAAIARRTGRH
                     PREVAYDLMLESDGRALLYFPLLGYADGDFGAIEAMLRDPDTVLGLGDGGAHCGVLCD
                     ASLPTYMLTHWARDRTRGARLSIEEVVHHQTRRTAALYGFDDRGLVAPGYLADLNIID
                     FDALRIAPPEMVYDLPAGGRRLIQRAEGY"
BASE COUNT         2251 a         4307 c         4755 g         2357 t
ORIGIN      
        1 cgctgggcag gcgtttcccg acgagacctt caccaacgac ggtcgctccg ggtccgatca
       61 catggaccac gccaggccac tcatcgaacg gtgggtcgag caacgaaccc ggtggggatt
      121 ctccgagtgg tattcggcgg tgtactacca ggaggacctc gaagcggcga tcaccctggc
      181 cgagttctcc gacgacgagg acctggcgac gctgggtgcg atcgccgccg acctggtgct
      241 ctacgacatt gcgggacact ccgtcggaag cttcgtcgcc gcgacccagg gccgcaccta
      301 caagaaggac aagatgtcgg gccccgacac cgacacctgg gacctcgcga ccctggtatt
      361 cggcaccgag gtcgcctacg gctcggcaac cggcagcgtg tatctggcga gcgcggcgaa
      421 gtaccgcccg ccagcggtcc tcggagcgat tcacgacgca gcgtccggtg cggccacgga
      481 gccgacggtc gagcgggcac gccacagcct cgccctcgac ccgctcgccg aggtgagcga
      541 cagccccgag gctccgatgg ggctggcctt cgacgaccac gacaacctga tgacctactg
      601 ggccatggcg gcgatgaccc cgtggcagac cgtcatcgaa tcgaccgacg agatgaccgc
      661 ctacaacctg tgggactcca agttgtttgc cccgttcgca ccgtttcgcc cgatcgtgga
      721 agccgccgat gccgacacgg tgaggggcct ggcccgatcg ctggcaccgc agctcaacat
      781 cggcctgttg tcggaggcga acacctacac gtggcgttcg ggtggcacgg cgcagcgggc
      841 gatgctgtcg acggtgcagg actatcgcaa ggggcaggcg agccaacaac accacatctg
      901 gcaggcgacc ctttcgtcgg aggcgatcgt gttcacgacc cacccgcggg tgccgaccga
      961 ggcaggtgtg ccgtggcgct cgaacagcga cgactggacc ggcggtgcgt cgatgccgcg
     1021 cagcgcccag cacgagaacc tgaacgtatc gatctacgcg ccgctgtacg catctggtga
     1081 caaccttcag ggttcctacg tggagtcgac ccatgcgtac tttccacagg agcgtttcga
     1141 cgaggtcacc acggtcggca actgggttct cggtcgcgtc ggtgacaact acgtggcgct
     1201 gtattcgtgg cgtccgctga ggtgggtgag ctatgacccc gccgagtacg acaccgacaa
     1261 gatgaccgag ccgttcgagt tggtggccga cggtgggccg aacaacgtgt ggatcaccga
     1321 gctgggctcg gccggtgaat acgccacgtt cgaagcgttc gttgcggcga tcaccgcaaa
     1381 cgagccggtg gtgaccccgc tcggcgatgc cgcggtgttt tccaccgggt tcgatgttga
     1441 gtgggtatcg ccgtcgcagg gactcgtcga gttcgggtgg gaacgtccgt tcaccgtcgg
     1501 cacaccagac gccgctacga cggtggagct atcggagtac ccgcgcatcg acgccccctg
     1561 ggcgcaggtt gcgttcgact cgaccgccta cgacatcgag gcgggcggcg agaccctgca
     1621 tctcgacgtc gtcgcgcgga cccgtcgatc cagcggcgat ctgtaatcgc cccagcatcg
     1681 ccgcgggtgc cgttcggcga acaggaccgg gcgcgccagc gggtcggatg tgacccgcta
     1741 gcgcgcccag tccatgcccg cgtccgtgtt cagggtcgat cgagcgggcc gcagaggtcg
     1801 acgatcgcgt cggcggtgag cgcgccgatg gccgttgggc tgagcgcggc ctgccgggcg
     1861 gacatcgctc gtcgtgaggc gttgagcgtc gcggcgtcga ggccgtcgat cgccgcccgc
     1921 cacgacccga cgctttggcc ggcacggatc gccgcatcgc cgaggtactc gtgcagatac
     1981 ggccagtccg atacgagtgc cggtgtggcc aggccgacga ggtcggaggc cgtgccggtg
     2041 ccgagcatcg aatgttcgat gaacggcagc gcgatcacat cgcaggcggc gatacgaagg
     2101 tcgtaggtct gccgatcgac attgtcgtac cggtgcccga cgatgcgggg atcgctcgac
     2161 agctcatcga ggaacgaatg cgaccagatc accaactcca cgtcgtcccg cgtgcaccct
     2221 tccaccgcct cgatcacgac gtccaggtgc ttttggcgcc gcggagcgcc gatcacgccg
     2281 attcgcaacg ccggggtcgt gtcgagtccg acccgtttcg ctgcctcatc gcggtcgatc
     2341 gagtggagcg acgggtcggc gagttcgccg aaatgaggat gagggatgac gacgtggcgg
     2401 cacggcgcgg cgaattcata gcgcgaggtc gcgaggtcgc gcccccactc cgaatggtgg
     2461 atcgcgagat cacaggcctg ggcccacacc tggtagatcg gttcatagag gccggggttg
     2521 tcgagatgcg gggtcaggtt gtgctgggtc catgcgacct tgacgccttc gtatcgaatc
     2581 gcttcggcaa ttcgccggtg ttgttcgccg gtgagctcca acccgaacgc ccactcgggc
     2641 cagtgcagat ggaagatgtc gccgagtgag gtgcgctcga tgcggtcctc ccagttccac
     2701 ccgcgcgtcc gaagccggcc ggtgccgggg gtgtcgatcg atccgtagac cgcccggatg
     2761 tagtggtggt cgaggtcggt cgttgtgacg accggaaccg gccggacgct ccagcgcagc
     2821 tcgatcgatt cggccggctc caccatcaac tccgcgacct cggtggccgg tccccacggc
     2881 gacgcccatt cgtgcatccc gtcgacctcg acgtgttcga tccgtaccgc cgtcgacgat
     2941 tcctcgacgg cgatgctgag cggtcgatcg aaggacggaa cgagaaccga gaccgcctcg
     3001 ggggtctcgt cgaatcgaag cgactcggtg agctccagcg cgccgtcgac gacccgatag
     3061 gtcgcgtggc gtgttgccga gatcggcggg cacggccgcg ggtcgtcgat gccgtgcaac
     3121 gcgccgaaac cctcgtggca caccgacacc gatccttggc gccactcgat cgacgtcggt
     3181 gtgcccgtcg gggcgaggcg ccgctggggg ttgagcccgt caccgctgcg atggaccagg
     3241 gggacgaacg tggccagggc cgaatcgacc ggcgaaccga acaagccggg acgccgaggg
     3301 cttggggcgt aatcgctcga cgggccggag acgaagggaa cgacgaactc gagggcgtcg
     3361 tcccggtagg cccacaccgc cgcgtgctgg tcgaggcgga cgagttggtc gcggggttcc
     3421 aggtcccgtg aagtggcacc gtcgatgttg aatgatccgg tcggttcgtc gagggcgtcg
     3481 tcgacgcgag cccaccgtgc ggcggcctcg gcgagtttgc cgaggacgtc gaaggtcatc
     3541 tgcaggcgac ggtgcggacc gcggtagccc tcggtcgcgc cgaaccgatg ggcggtgatg
     3601 accccgtctc ggtcaaacca gcgttcgggg aagccgtcgg cggcgatcgc tgccagtcgg
     3661 agataccagt cgcgttgggt cgcgctcccc gcgatggcga ggcgttgccc gtctgcggtg
     3721 accagcgccg cgagttcgat gctgagggct tgggagagtg ccccggttga acgcccccac
     3781 ccgaacgcgc tcgcgtcgct cgcagcgacg gcctccacga gcgcgagcgc ctcgatcatg
     3841 ccgtcgcgcc agacatggcc gagtcggtcg gcaaagggtt cgcagaacag gtagaggtct
     3901 gcggtgtaga tgtcgtatct gccatcgccg tcgtgggaat cgtcgaggaa accgcgcgga
     3961 ttctcgccca gcagggtccg cactcccgcc aacagcgccg cgaggccggc gtcatcgtcg
     4021 agcagaccca ggcgctcacg agcgatctcg catcggctga gaacggccag gtagttgcgc
     4081 ggcaacgcat cgaggagcgc aagaacctcg gtgctgtcgc aggccagacg gaggttgtcg
     4141 agcgtggcgg cgtcgaggcc gtcgagcgct tcgttgtggt cgaacccgcc gaagcggccg
     4201 atcgtctcgg caacccgata gctgaagaac gtgtgggtgg cggccccatc gacctgcgcc
     4261 agcaccgcgc ggattgcatc gatgacggcg gtgccggcga cttcggccac cccggcctcg
     4321 tggagccagc cgagggtgaa cgccaggtcg gccaccgcgt cggccccgac gatgtggccc
     4381 gcgaacaatc ggggaagttg ccagttcggc gcgtggcgtt cgaccaacgc ctcgagggtg
     4441 ttcaccacgg tgccttgaag cccaagaacg tctcgcacgg acggttgcct tcctcggtag
     4501 ggtgacctga tggctgtgcc ccgtgtctac atccacgagt tcatcgacat catcggtcac
     4561 ggtcgggcga agtacatgta ccacatgacc gccaactggg tgccggtcgg catcgccgaa
     4621 cgaaaccagc gctgcttcgg cgtgtggggc gtcgtcgggt cgaccggcac ctggcctcag
     4681 gtggtcaacc tgtgggagta cgacgactgg gcggcgctcg gccacaactt cgaggtggaa
     4741 caacggggcc ttgggctgca ggatccgtcg ctggaacggt ggtggcgggc ggcggccgag
     4801 ttccgttccg gaggggtcga tcggattctc gtcgcggcgc cgtggtcgcc gtcggtcgcc
     4861 gaccacgaac ggagcttcac ggctccgccg gccggctacg tccacgaaca ctttcgcgtg
     4921 aagcccggcc atgacctgac gctgctcgat gcgatacgcg acgccggtat cgatgcgtac
     4981 gagacggcgg ggctacgcct ggtcggagcg tttcggcgag cgatggccgg cgacgatgaa
     5041 gtcatcgcca tctgggcctt cgcgaactgg caggtgtggg ccgacttcga agcagcgctg
     5101 gatccactgt cgacgcagtc cggcgacatc gactgcgagt ctccgtgcgg cctcctgcac
     5161 gcctggttcg ggtcgattgc gggtatcgtt cggggtcgcg agcggattct ccttgcagac
     5221 gcaccgctgt caccgttgag aatcggtcgc cagcctgatg aatcggatcg gaccactgat
     5281 gcctgaagtg ccgatgagtg aagtgccgag gagtgaagtg ccggcagccc acgttgatgt
     5341 gcggtcgtca ccgatgattc gccaccctcg gttacaccca ccgatccccg tgttcggcca
     5401 ctacggcgaa aagccgcgct ggcgtggccg gattcattcg gccgcgttcc tcgtctcgat
     5461 cccggccggg ctgtggctca tgtcggtggc cgactccgcc gcagcacgcg tcgcggtcgg
     5521 catctactgg ctgtcgctgg cgggcctgtt cggcacgagc gcgtcctacc acctgttcgc
     5581 ccattcggcc ggcgcggtga agtggatgcg gcgggccgat cactcgatga tcttcgtgct
     5641 gatcgccgga acctatacgc cgttttgtct ggtcgtgctc ccgaagccgt ggggagtgcc
     5701 gatgctggcg ttgctgtgga cgatcgccgt ggcgggcatc gtgatgaaga tggtgcgtgt
     5761 cggaaccgac aagtcgcagg cctctgggtc gtggctctat ggggtgatgg ggtggatcgc
     5821 ggtgatcgcg ttgcccaagc tggtccagga actgggctgg agtcgcatgg cactgctgtt
     5881 tgcaggcggc gtgatctact cggtgggcgc ggtgatcatg agcacgaaga agctcgacct
     5941 gcgccccgca accttcggct atcacgaggt gtggcacgcg atgacggtcg cggcgagcgc
     6001 ctgtcacttc gtactggtcg ccatggtggt gcgctgaacc gcagcgggat cgccccgccg
     6061 gattcggccc ttccgttcag accagtgccg cctcgacgcg cccagtgagg cgctctccga
     6121 cgaccgcggc agcggcgggt ggcagcgagg ccgcgtatcg ttccagacgc tcaagctctc
     6181 cgaatcttcg gaacgtgtac tcgaaatagt tgtcggggat gtccgtcgag ccggtcgcca
     6241 gcgactcggt gatgaggccc atcgcactgg tcaggtcacc ctggtaccag cgcactgcgg
     6301 catcgatgag tgggcgatgg tcggggtgga cgcgagcgag ctcgagggct gcgagctcgt
     6361 cgaaccgttg ccggtacagc agcgcccagg cgacggtggt gtagacctcg ctcgaaacga
     6421 gccagccgtg ttcggaccag atgacggcca acaggggttc caagccacgg tcctcatcgc
     6481 cgtcgaggag ccgttcgtgc gcgatccgga tgtcctgttg gcggcgttcg cgtgcggtga
     6541 acgcgaatgc ggtcgagttg gtcacgatca tcagtacgag cacgctgaac acgatgtagg
     6601 ggtgctcgcg accggcgaac caccaagcgg cggcacagat ggcggcggtt gcggcgatca
     6661 accagccgat cgccgcgccg cgccgccgcg atgccatctc gaccagctcg ctggtgatgt
     6721 ggccgccgtc gagcggcacg atgggaagga ggttgaacag gctccaccac acgttcacga
     6781 accacgatgc cgcgctgaac cacacgatct ggcggccgtg gatcggaatg agcgctgagt
     6841 tgatcgtgat cccgagcagg atcgcggcgg cgggtcccgc gagggagacg ccgatcgact
     6901 gccacggcga gatgcgaccc cgctcatcgg ttgaggtgag ccccccgaac gcgttcaacg
     6961 aaactccggg atggaatccg caggcccgta gcgccgcgac gtggccggcc tcgtggacca
     7021 gcaccgaaac ggtcacgagg accagccagg cggtcagcat ccacgggtct cggccaaagt
     7081 ggccaccgaa gatcaacggc aacgccacga ccatgagcgg gaacgtgagc ccgatcctga
     7141 cgggggtgcc gaagacccgg aatcgaagtg caggtcctcg gccgcgttgc atcgctccag
     7201 tttgctgtga attcggtcga ggcgttgcgc gtttctgaga gtcggacgac gactatcggt
     7261 gaggcggcgc gtcgaggcct cccatggtgg cgatcgagtc gagcgaacgg atcgtgtcgt
     7321 tgaggtgcgt acacgtggag gtcccgcgga agtcgtgccg tacgagagac cgcagtttgc
     7381 cgacccgctg gccgacgcat cgttgtgcgg acgccagggc cgacggacac tccatccacg
     7441 gcaacacccg ggcctcggca tcgaggtcgg cgatgactcc gtcgcggagg gtgccgcgta
     7501 cgaggtactc gtggagcacc cgctcggtgg tcccgaagtc ggcgtgcgag tcgcgaaagt
     7561 gggtctcgaa ccgccgctcg ccgccatcgt cgggtccgag gtcgattcgc cgaacccgtc
     7621 gggttgtgtt ggcgctgagc ggcggaatcg ggtgccacga cccggtcgcc tgtggggcct
     7681 gggtgtgagc ggtagtccac aacgccggtg cgggcggacc taccggtgtg agcatctcgc
     7741 cgtcgttgcg cagcgaggaa atcagcgtcg cctcctcggc ccatcccgca caggtgtcgg
     7801 cctggaaatc gatcgcttcg gggttggagg cgaagatctc tgagatcgcg tcgacaccgg
     7861 tgcgcgagtc ctcgtactgc tccgcgtacc cgccgaccag cgtgataccg ggcaggtcgt
     7921 cgaggagaaa catggccagc gccgaacagc cgccgacctg gcgaagggcc gagtcgagtc
     7981 gcgcccggaa tccacctgcg gtgctgtgcc cgacgagtgc ggacaactcg agggcgccgt
     8041 tcggtgcgcc gctgtcgtgg accgagatgg cgctcagcgt gcgcgccatc gcgtcggtct
     8101 cggcactcag ggtcaccgtc gcctcgacga cgggttggcg atctgtgtcg attcgcacgg
     8161 atcgtgcgac ggcctcgcag cgcaccggcg ccgatccgag cccattgggt gaactcgaat
     8221 cggcgtggaa cgtccatcgg gtccaccctg gagcgaggac aggggtggac cgaagtggat
     8281 tctgcggtcc gttgcgtccg acggtgaacc ccatgggaac ctcctaccgc ctcgttgcgg
     8341 acccgtggtt tcagctgttc agcaacgcgc gggcgacagc gatcgtctgt tgttcgtcgg
     8401 tgccggcgta gatctgcagg accttcgcgt cgcgggcgag ttgttcgacc tggaactcgc
     8461 tcatgtagcc gttgccgccg aagagctgca ccgcttcaag cgcgacggcg gtcgcctgct
     8521 gggccgaata gagcttcatc gccgaggcct cggcaaaggt catggtctta cccgctttgg
     8581 cgagttcgat gatgcgaaac accaggttct gcacgttcat gcgcgccacc tccatgaagg
     8641 ccagcttctg ttggatgagc tggaagtcgc cgatgcgttg gccgaactgc ttgcgttcct
     8701 tggcgtagtc gatcgacagg tcgaggcact gctcgatgat gcccagcgcc attgcggcga
     8761 ccccggaacg ttcctgctgg aaggtcgcct tggccccttc gcggccgccc ttcgcgacgt
     8821 tctcggtctc gccgatgagg cgatcgatgc cgacacggac gtcggcgagg aagagttcgc
     8881 cggtggggga ggagtgcatt cccatcttgc gcagcggctt ggactgctcg aggcccgcca
     8941 tgccggagtc gagcacgaaa ctcagcacct tgcggtcctt cgggtcgttg ccctcgtcga
     9001 gcttggcgat aaacacgatc gtgtcggcgt agggaccgtt ggtgatgaac gtcttgttgc
     9061 cgttgaggac gtattcgtca ccgtcgcgcc gagcggtgga cagcatcgaa ccgaaggcgt
     9121 cggagccggc gttgggttcg gtgatcgccc atgctcccac cttgcgcatc gtcaacaggt
     9181 cggggaccca acgctccttt tgcgcgatcg tgcccttcga gctgatggcc gcggcggtga
     9241 ggccggtgga gacccccatc gcggtgacga tgccggggca gtgccgacac agctcgatga
     9301 tgggaatcag tgtgaacccg ggatcgctcg tggcatcccg caccggcttg tcggcctcgg
     9361 aggcttcgcc ggctgccacc tgcctttcga actcgatgcg cttggagaat gtcgcgtcgg
     9421 ccatcgcatc catgccgaag gttgaataca tcttgcgcat gaactcgtag gggggcaggt
     9481 cgccgtgttc gagttcctct ttgatcggca ggagttcctt ggcgaggaag tcgcgcatgg
     9541 cgccccgaag catgaggtgt tcttcagacc attcgtacat gcggggattg tacctgacgg
     9601 acttgaccga ccggtcaagt ccgtcaggtc cgtcaggtca ggaaatgccg tcggctggat
     9661 tcgagccgag gctcgggctg ggcccaggtc gggttcaggc gagttctgct tcgatcgctg
     9721 cgatcaccgc ggggtcgtcg ggggccaccg tcggagcgaa ccgggcgacg acgtcgccgc
     9781 tggggttcac gagaaacttc tcgaagttcc atcggacgtc cccggtgtgt ccgtcggaaa
     9841 catcggccga tcgttcgagt tcggcgaaca aggagtgctg cgattcgccg ttgacgtcga
     9901 tcttttccga cagcgggaac gtcacgccgt agttcacctg gcagaactcg gagatctcat
     9961 cggcggtgcc gggttcctgc tcgagaaact ggttgcacgg gaatccgaca acggtgaagt
    10021 cctcgtcgcc gaagcggtcc tgaaggccct ggagctgttc gtactgcggg gtcaggccgc
    10081 acttggacgc ggtgttcacg aacaatgccg tcttgccggc gatgtcgctc atgagatcgg
    10141 gcgtaccgtt caacgcggaa atggtgtggt cataggcgga catcgtgact cctcgtgtgg
    10201 tcatcggtcg ggcaacgtgt acccaacagc agtattccct acgcttgcac ccatgatgtg
    10261 gcgacaccga actgctcgtc ccctgggggc cacgaaccga ggggcagcgt tcgcgtcggc
    10321 gctggccgcg attgcgatcg tgggcgctac ggggtgtgcc aagagcccga tgcaagcgtt
    10381 cgaggacctg caggtcgagg agacacccgt cgaggtggtt gcctcgtcgc tgctgcgcct
    10441 cgatggacgc gtcgcgctgg gaccagatct cacgcgcacg ctcttgaagg tccaactcgt
    10501 tccggagggg tattcgtggg ccgacgccga ctgcgcggtc gatgcggtga ttggtgtgat
    10561 gggagccgaa gccttcgcgg tggtcccggc gggaaagatc cgcgcgatca cctgggacta
    10621 tccgggtagc gacgaagccg cgttggcgtg tcgctcgtcg gagaccgagg cccgtcgaaa
    10681 tcactcggtc accaaatccg acatcaccat cgccgaccca cagcccggcc ccgacccgga
    10741 tccggcggcg gttcgtgagc tccaggcccg cgggttctcg ttgggtgccg aacgcgccgg
    10801 gctgacgctc gaggaacggg aatgcatcct caaaccggtg atcggcgatt ggaccgacgc
    10861 cgattacgtc ctgctcgccc agcgctcgaa gcagatacgc caagacgacg tcgtcgctgc
    10921 ggtcgacgag tgtctcgacg acgaccgtgc cgccgaggcg gcgacaacag cggtgccgtc
    10981 gctgttggcc gccaacgaat gggcacagga agaatcggac cgggtcgacg cgctgaagac
    11041 cgccgcaggc atcgacccgg acagcggtga accgatcgtt acagatccgg cagcggcatc
    11101 tccagattcg gagaagtgag ccccccatcg atctcgagga tcttgccggt catgaacgac
    11161 ccggcgggtg agcacaggta gagaactccg agtgcgatgt cgagcgggtc gccgagacgg
    11221 cgcagcggtg tgcccgactc gagcgcagtc cgcaggtcat cgttctcgat caccatttcc
    11281 agcgcgctcg tggcggtgga accgacggcg attccgttca cccgaatccg cggggcgcac
    11341 tcggtcgcca tcaaccgggt gaggtgactc agcgccgcct tcgcggtgcc gtaggccgcc
    11401 atgccgcgat cgctcagccg gccgatcgcg gaactgatgt tgacgatcga cccgcaagcc
    11461 gcatgagtac cggggtcgtt ggcgtcgcca ccgatggtcg gttgttgaag catcgccggg
    11521 atcgccgcct tggacagctc gaatgcagtc gtgacgttga aatggaaggc ctgctcgagc
    11581 atccgactcg acgtgtcgag caggggtttc gggaagcttc cgcccacgtt gttgacgacg
    11641 atgtcgaggc ggccgaactc gtcgagcgcg acgtcgacga ggccccgcac ggcgtcgagg
    11701 tcgttgaggt cggcgggcac gcacaccgcg cgccggccgc acgccgtaat ccgatctgca
    11761 acctcgtcga gttgttctgg cgaccgggcg gagatgacga catcggcccc ggcctcggcg
    11821 aatgccatgg cgctggcggc gccgatgcct cgtccggctc cggtgacgat ggcgacctgg
    11881 tcggatacca ggaaatggtc ggtgatcatg atgtcccctc tcggccgccc acactttcgc
    11941 gcatcggaga tgctcgcccg ctcactcgcg gcgccgagtt gcttgaccgg tcgaatcggg
    12001 gccggtctac ttatggggtg cgacccgggg gatcgaggcc gccggtcagg ggtgtgccat
    12061 gcatgatgtc gtgatccgcg acgccacaat cgtcgacggc tccggagcca ggtcgttcac
    12121 cggcgacatc gcgatcgagg gcgagcgaat cgtggcggtc ggggtcgtcg agggaaccgg
    12181 tcgacgtgag atcgatgcag ccgggctgct cgtcaccccg ggatgggtgg acatccacac
    12241 ccactacgac ggacaggtca cgtgggatcc ggaggtcacg ccgtcgggct ggcacggcgt
    12301 caccaccgtc gtgatgggca attgcggtgt cggtttcgct ccggtcgatc cggcacggcg
    12361 cgactttctc atccagttga tggaaggggt cgaggacatt cccggaacgg cacttgccga
    12421 agggatgagt tggaactggg agaccttcga ggagtacctc gacgaagtcg catcgatgcc
    12481 gcgggtgatg gacgtagcgg cgctcgttcc tcacggggcg gttcgcgcct acgtgatggg
    12541 cgacgacgcg gcggtgcacg atgccgccac tcccgaacag atcgaccaga tgcgtgccat
    12601 cgtcgccgac gcactcgccg cgggcgcggt cgggttctcg acgacccgca cgatgctgca
    12661 ccgggcaaag gacggcgaac tcgcggcggg cacgttggcg gaggtcgacg aactcgtcgg
    12721 catcggccag gcgctggcgg acaccggcgc cggattgttc tcggtcgcct ccgacatgtt
    12781 cgacgcccgc tcggagttcg agtggatgac cacggtgtcg cgcgagtccg gtcggcctgt
    12841 gaccttcggg tgtctgcaga gcccggtgca gtcggacaac tgggtcgagt tgttggatct
    12901 ggcccgaagc gccacggctg agggagcgcg cattgtgcct caggtggctg gcaagcccgc
    12961 gagcgtcatg ttcggtttcg agtcgtcgtt gcacccattc ctgcatcacc gcgcgtacaa
    13021 ggcgattgcg catctcccgt tcgccgaacg aatcgccaag ctgaaatcgc cggaggtgcg
    13081 tgaggcgatc ctcgccgaac gggtcgagta tcggggattt caagcgatcc tgttcgaggg
    13141 gttcgaccgg ctctatccgc tcggtgatcc gccgcaatac gagccggaac ccgaggccag
    13201 cgtcgcggcg atcgcccgtc gaacgggtcg ccatccccgc gaggtggcct acgacctcat
    13261 gttggaatcc gacgggagag ccttgttgta tttcccgctg ttgggttatg ccgacggcga
    13321 ctttggagcc atcgaggcga tgttgcgcga cccggacacg gtgctcggcc tcggcgatgg
    13381 cggcgcccat tgtggggtgc tctgcgacgc aagcctgccc acctacatgc tcacccactg
    13441 ggcgcgggac cggacccgcg gagcgcggct gagtatcgag gaagtggtgc accatcagac
    13501 ccgccgcacc gccgcgctct acgggttcga cgatcggggg ctggtcgccc ccgggtacct
    13561 cgccgacctg aacatcatcg acttcgacgc actgaggatc gctccccctg aaatggtcta
    13621 cgacctgccc gccggtggtc ggcggttgat ccagcgtgcg gagggatacg
//
LOCUS       DAOOMA010000008        13339 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_5099_length_13339_cov_4.293135, whole genome shotgun sequence.
ACCESSION   DAOOMA010000008 DAOOMA010000000
VERSION     DAOOMA010000008.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 13339)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13339)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13339
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5099_length_13339_cov_4.293135"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..204
                     /locus_tag="PLP95_00490"
     CDS             <1..204
                     /locus_tag="PLP95_00490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015439769.1"
                     /note="Members of this family of hydrolases with an active
                     site Cys residue belong to MEROPS family C26; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HPB44312.1"
                     /translation="EATRYHSLCVRPDSVPDCLEVTAETDDGIIMGLRHRELNVEGVQ
                     FHPESILTVAGHQMIQNFLDRCG"
     gene            complement(235..1875)
                     /locus_tag="PLP95_00495"
     CDS             complement(235..1875)
                     /locus_tag="PLP95_00495"
                     /EC_number="6.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD+ synthase"
                     /protein_id="HPB44313.1"
                     /translation="MTIVRIALCQINPTVGALVGNTDRIIEVLAEAESAGAHLAVFGE
                     LALCGYPPEDLVLKRSFVADCEAQLQRLAAAAGSCGAVVGLPTVSADGSLRNTAAICR
                     HGRVEYRYDKQELPTYGPFDEDRWFRPGQVDQPLFDLDGIDLDGIAVGVSVCEDIWIA
                     DGPVFRQGQSGARLLVNINASPYHHDKLDHREEMLRERSIAAGAPIVYVNQVGGQDEL
                     VFDGGSFVVGDDGELICRFPQFVEHVELVDIDLDGASQKADLIAPPLDDIAEIWHALV
                     MGTRDYVHKNGFSDVVIGLSGGVDSSIVAAIAVDAFGPEHVHGVLMPSRFSSDHSISD
                     AVRLAETLGIDHRTIPIEPGHLALETMLAESFAGRAHDLTEENLQSRIRGITLMALSN
                     KFGWMVLTTGNKSESAVGYSTLYGDTAGAIAVIKDVYKTHIYRLCRWRNSRAGREIIP
                     ESVLTKAPSAELRPDQRDDQSLPPYDVLDAILYAYIEGDRTLADLIGEGYDPETVERI
                     CRLVDISEYKRRQSPFGLRVSSKSFGKDRRIPMTNRYR"
     gene            complement(1954..2970)
                     /gene="glnII"
                     /locus_tag="PLP95_00500"
     CDS             complement(1954..2970)
                     /gene="glnII"
                     /locus_tag="PLP95_00500"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase GlnII"
                     /protein_id="HPB44314.1"
                     /translation="MPYRAEYIWIDGTEPTPLMRSKTKIIEDGGEPGIWGFDGSSTNQ
                     AAGSDSDCVLNPVFTCPDPTRGPGDILVLCEVLLPGTMEPHSTNTRRYCAEVDAKFAD
                     QAPMFGMEQEYTLFQEGRPLGWPKEGYYYPAPQGPYYCGVGSVEIAGRDFVEAHTEAC
                     IAAGLKICGTNAEVMLGQWEFQIGIAGTLEVADHIWMARYLLYRIGEDFGVNASISAK
                     PIKGDWNGAGMHTNFSTAAMREGWDPIIAACESLGAPGKVEEHLAGYGHGYEDRLTGQ
                     HETAHFSEYRYGVSDRGASVRIPWQVGVDKKGYIEDRRPNANADPYMVSALITNTVCS
                     ALAK"
     gene            3181..4515
                     /locus_tag="PLP95_00505"
     CDS             3181..4515
                     /locus_tag="PLP95_00505"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020378967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamate-ammonia ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase family protein"
                     /protein_id="HPB44315.1"
                     /translation="MGHHHDYVLRTLEERKVHLVRLWFCDVLGQLKSVAISPVELETA
                     FEDGIQFDGSAVDGFSRVQESDVLAMPDPSSFQLMTWGDGEVSARMFCDIVHLDGTPF
                     EGDPRQVLKRNLQRARDRGFELMCAPEVEYFYLASDDPSQPPQTLDNGSYFDLTTSDI
                     ASDIRRATIHQLENTGIPVEYSFHEDAPSQHEIDLRYDDALAMADAIMTLRVVVKEVA
                     YQHGVHATFMPKPIAGVQGSGMHTHISLFRDEENAFYDSEGDANLSEVARQFIAGLLR
                     HAPEITAVTNQLVNSYKRLIGGFEAPVHVSWARNNRSALVRVPVSKPNKEAAGTRIEY
                     RALDPACNPYLAFSVLLAAGLAGIEGNYELAPETTGNLFELAEQGNLDESIELLPSSL
                     AEALDLMEQSELAREALGEHIFEWFLRNKRAEWAAYKAHVSQFELDRYLGNW"
     gene            4527..5198
                     /locus_tag="PLP95_00510"
     CDS             4527..5198
                     /locus_tag="PLP95_00510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006303741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HPB44316.1"
                     /translation="MEPLLCWPDPVPPEIVRCLELASIPWGTATTDTDIAAATWAGAI
                     VDASNDTDEAFTYARRLSATNLGVAPLLLLVRGSQLPDLDRHHDLFEDFCVAPFHPVE
                     FEARVKHLLTRQGLLSDDSEIIQYKSLVLNLETYQASIDGTPLDLTYMEYELLKFLAQ
                     NPGRVFTRETLLSRVWGYEYYGGARTVDVHIRRLRAKLGEQHAQMIGTVRSVGYRFGK
                     SRWDS"
     gene            complement(5225..5383)
                     /locus_tag="PLP95_00515"
     CDS             complement(5225..5383)
                     /locus_tag="PLP95_00515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB44317.1"
                     /translation="MVGAIIIVIVLLVVIPVSVLVSGGALAGVIGFFVQKDVDDTNEG
                     SELIDLNG"
     gene            complement(5464..5808)
                     /locus_tag="PLP95_00520"
     CDS             complement(5464..5808)
                     /locus_tag="PLP95_00520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019239 - deaminase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rid family detoxifying hydrolase"
                     /protein_id="HPB44318.1"
                     /translation="MSTPVGPYTPIVRAGSFLYASGQLGLLDGALVEGVAAQTAQALR
                     NLAALVELEGATLDQVVKTTVFLADMDDFLTMNEAYVAGFGDHRPARSAVAVAALPLG
                     AAVEIEAVIHIG"
     gene            complement(5818..7401)
                     /locus_tag="PLP95_00525"
     CDS             complement(5818..7401)
                     /locus_tag="PLP95_00525"
                     /inference="COORDINATES: protein motif:HMM:NF013923.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HPB44319.1"
                     /translation="MQFELNGEQVEVADDGASLLEVLRDRLGVTSVKPGCNPQGQCGC
                     CTVLVDGEARVGCVTPARRVAGRSVVTLEGLSDERREAWGEALCATGGSQCGYCTPGI
                     VMRLEGTAGKGGDLCERAVVDRALAAHLCRCTGWQTIREAAVMVATGAEVRSESRDFD
                     AASRRATIEGHAHQRVGPDIGLGMGGFADDGAPSDAPVALLAADGTWTVAPTMTEARA
                     AIGKIQGRNTTVDAVAPIEVPDGDWALTLQTNWVEPAYLETDAAWAIPGSDPTDPLAN
                     GGAFGGKVDSPVPGDAVELAAREGGPVRLRWSREDVVRRGPKRPPMALGMRSDGSGVV
                     RVARTPGIVEAIVSVAPLIEVEEVDIVGPPTSTAIRGAGWAEVAAVLASLESLATDQP
                     GTATVRSPEGASATVRIDDEGAHVVVRCGEVLDTSVVRAYAIGAVHMGLGLVTSEQLS
                     VDDQGNPLNLTIRSFGVVRPVDMIPVEVTVVDEPGIEPVSGSDAVFAATAAAIWTLQG
                     TPGVWPTKLPVRFSDPATA"
     gene            complement(7419..7739)
                     /gene="erpA"
                     /locus_tag="PLP95_00530"
     CDS             complement(7419..7739)
                     /gene="erpA"
                     /locus_tag="PLP95_00530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012223429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HPB44320.1"
                     /translation="MITLTDSAATKVRQLIDQEGDESLALRVAVRPGGCSGFSYEMYF
                     DNEIKTEDQTNDFDGVKVVVDPTSLPLLVGAELDYKDGLEKSGFEINNPNASKTCGCG
                     SSFS"
     gene            7900..9162
                     /locus_tag="PLP95_00535"
     CDS             7900..9162
                     /locus_tag="PLP95_00535"
                     /inference="COORDINATES: protein motif:HMM:NF022077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mrp/NBP35 family ATP-binding protein"
                     /protein_id="HPB44321.1"
                     /translation="MGIPSDSPRPSTSEITGLLRGVIDPELGSDIVALGMVKDVRVGD
                     DGLVVVKIALTTAGCPLRAQIQRDVRDRVLSLPGVTKVKLDWTELDADEKAAAMAVAR
                     RNIAEHPPETAVPSTTKVLMVASGKGGVGKSAVTSNLATAIAALGYRVGVIDADIWGF
                     SQPRMLGVHGRLEAHERTAGSVGGSDASDATIEPRIRTVGDGELRVVSMGFLVQNEED
                     ALMWRGLILQRAVQHFLEDVAWGSDLDYLLIDMPPGTGDVQMGLAKLLPRAEMLVVTT
                     PSLSAQKVAARAVSMGRKNYLRVLGIIENMSYFVAPSGERFPIFGSGGGDRLAATTGA
                     PLLTRIPIENAVAEGGDTGSPIALGDGPAAEAFRDLARRITTDIAPPTDLTGCSARLL
                     DAVAAALDAHDSAERDRCDDSVNSDVSS"
     gene            complement(9140..9979)
                     /locus_tag="PLP95_00540"
     CDS             complement(9140..9979)
                     /locus_tag="PLP95_00540"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HPB44322.1"
                     /translation="MSLSLQVDDPHEIPIRDAATVMLVRDGRHGVEVFMLRRNLNSDF
                     VGGAYVFPGGAVDPGDSDRAIEALSVGLSDAQASELLGVGRGGLAYWVAAIRESFEEA
                     GLLLAVDEDDRMVGFANPEVAGRFHRHRIDVDTAVRALRDVVREERLRLATDRMHYFS
                     RWITPLGSPRRYDTRFFVAHAPEGQVPSHDDRETIANLWIRPTEALERNEAGDFTLIF
                     PTVRTLEVLARFDSAEAVVAHARSIAEFPAILPRIVEDGGGLRIVLPGDEVYDAFSSK
                     RLN"
     gene            complement(9984..10520)
                     /locus_tag="PLP95_00545"
     CDS             complement(9984..10520)
                     /locus_tag="PLP95_00545"
                     /inference="COORDINATES: protein motif:HMM:NF016102.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FxsA family protein"
                     /protein_id="HPB44323.1"
                     /translation="MTFVLLALLALFVVEVTVMIAVANATSVITMFLALTLLCAAGAW
                     VVKRQGFATWRRINDGFARGEMPTDAVLDGLIWMVAGALLLVPGFVSDLAALVLVLPS
                     TRALLRPLVVRRAQVRAESTFNGSAVRFATFGFSTARGGFDDDFLRRRPRHDDGIIDL
                     DAEEIFLDEPRGELGPGT"
     gene            complement(10588..10851)
                     /locus_tag="PLP95_00550"
     CDS             complement(10588..10851)
                     /locus_tag="PLP95_00550"
                     /inference="COORDINATES: protein motif:HMM:NF014520.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WhiB family transcriptional regulator"
                     /protein_id="HPB44324.1"
                     /translation="MSAQPSFDSWHHRGACRGPQSAVFFPPPHFERKHDRIAREHRAK
                     EICAQCPVVSDCLSFALEIREPHGIWGGTNEVERRAMLEVRSA"
     gene            complement(10930..12270)
                     /locus_tag="PLP95_00555"
     CDS             complement(10930..12270)
                     /locus_tag="PLP95_00555"
                     /inference="COORDINATES: protein motif:HMM:NF014062.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HPB44325.1"
                     /translation="MRTVLSRTIFAVLLALFSSLGVAASARPASAAVAPCPNENGCVR
                     AITVNGFIDQINADFIRRAASEVADVPGYSAIILVIDSEGSVISAEELNDLVVDLVDL
                     PLKVTAWVGPSGAQALGGAAELVAALEPSMAPNTRIGDVGVPQLDQQRFGDLVTSTDT
                     GLRETVIDNDEAEQRSLSLRTDAILGDHALNRGDVAFKDVTDDEGRPKRELLTTNITI
                     GLPVTTQLLHTAASPAVAYLALAIAIGLLLFEFFTAGVGVAGVVGAICAVMAGYGLAE
                     LPLRWWALALCLFSAFAFAIDIQTAIPRLWTLIGLVSWSVGSLFLFDGLRAPWLALLT
                     GLGGMAVLMLSGMPSMVRSRFATPTLGRSWMIGKMGTAISDINPEGTVDVDGGIWRAI
                     TNRATPVMAGGELRVVGIDGMTLEIEPPEGGAIDYRDRRPAASDDPGDEPDSVT"
     gene            12292..13242
                     /locus_tag="PLP95_00560"
     CDS             12292..13242
                     /locus_tag="PLP95_00560"
                     /inference="COORDINATES: protein motif:HMM:NF014433.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsA-related P-loop ATPase"
                     /protein_id="HPB44326.1"
                     /translation="MDPAQFFTAPRLTIVAGKGGVGKTTVTATLALAAARAGLRVLVV
                     EIEGKFGLPALFDKPALSYEELVLFEDLHGAGSVQARTLTPDAALLEFLGDHGMRRLS
                     RRLADSGAVDIVATATPGIKDVLVLGKVKQLERSRTYDAIIVDAPAAGHAITFLSSAK
                     GLLDAVHVGPIESQAREVNDMLTDETRCQVVLVTLPEETPVNELVETAYNLEDRIGIK
                     LGPVVINGDYEHLDGIEVAPGDAAAAAGVTLDARLSADLAAASALRRERTAAQVSQIE
                     RLNRELPLPQLRLPFVFRTDLDLDALITLSDSVLDQVRAI"
BASE COUNT         2461 a         4500 c         4132 g         2246 t
ORIGIN      
        1 gaagcgaccc gttaccactc gttgtgcgtg cgccccgatt cggtgcccga ctgcctcgaa
       61 gtcaccgccg aaaccgacga cggcatcatc atgggcctgc gtcaccgtga actgaacgtc
      121 gagggtgtgc agttccaccc cgaatcgatc ctcaccgttg ccggccacca gatgatccag
      181 aacttcctcg accgctgcgg ctgaacctcg ctgcgttcga gccctcgagc gccgtcagcg
      241 gtaacgattc gtcatcggaa tccggcggtc cttgccgaac gatttcgaac tcacacgcaa
      301 cccgaacggg ctttggcgcc gcttgtactc cgagatgtcc acgagccggc agatgcgctc
      361 gaccgtttcc gggtcgtacc cctcgccgat caggtccgcg agcgtgcgat ccccttcgat
      421 gtacgcgtag aggatcgcgt cgagcacgtc gtagggcggc aggctctggt cgtcgcgctg
      481 gtcgggtcga agctctgccg acggtgcctt cgtcaacacg ctctcgggga tgatctcgcg
      541 gccggcgcgg gagttgcgcc aacgacagag ccggtaaata tgagtcttgt agacgtcctt
      601 gatcaccgcg atcgcacccg cggtatcgcc atacagcgtc gagtacccga ccgccgattc
      661 gctcttgttg ccggtggtca gcaccatcca accgaacttg ttcgacagcg ccatcagcgt
      721 gatcccgcga atcctcgact gcaggttctc ctcggtcagg tcgtgagcac gtccggcaaa
      781 ggactccgca agcatcgtct cgagcgccag gtgcccgggt tcgatcggga tggtgcggtg
      841 atcgataccg agggtctccg ccagacgcac tgcgtccgag atggaatgat ccgaactgaa
      901 cctcgacggc atgagcacgc cgtggacgtg ttcgggcccg aacgcgtcga ccgcgatcgc
      961 ggcgacgatc gacgaatcca caccgcccga cagtccgatg accacgtcgc tgaacccgtt
     1021 cttgtggacg tagtcgcggg tgcccatcac gagcgcatgc cagatttccg cgatgtcgtc
     1081 gaggggcggt gcgatcaggt ccgccttctg agacgcaccg tcgagatcga tgtcgaccaa
     1141 ttcgacgtgt tcgacgaact gggggaaacg acagatcagc tcgccgtcgt cgcccacgac
     1201 gaacgaaccg ccgtcgaaca ccaactcgtc ctggccgccg acctggttca cgtacacgat
     1261 cggtgcgccg gcggcgatgg agcgctcgcg caacatctcc tcgcgatggt cgagcttgtc
     1321 gtggtggtac ggcgacgcgt tgatgttcac caacaaccgc gctcccgact gtccctgacg
     1381 aaacaccggt ccatccgcga tccagatgtc ctcacagacc gacacgccga ccgcaatccc
     1441 gtcgagatcg atcccgtcga gatcgaacaa gggctggtcg acctgacccg gacgaaacca
     1501 ccggtcctcg tcgaagggac cataggtcgg cagttcctgc ttgtcgtagc ggtactcgac
     1561 gcgtccatga cgacagatcg ccgccgtgtt tcgaagcgag ccgtcggcgg aaacggttgg
     1621 cagtccgacg actgcgccgc aggaccccgc cgccgcagcg aggcgctgca actgcgcctc
     1681 gcagtcggcg acgaatgagc gcttcagcac caggtcctcg ggcgggtagc cgcagagcgc
     1741 gagttcgccg aacaccgcaa ggtgcgctcc ggcggactcg gcctcggcga gcacctcgat
     1801 gatccggtcg gtgttgccga cgagcgctcc caccgtcggg ttgatctgac acaaggcgat
     1861 gcgaacgatg gtcacggcga caacctaccg gggccgcgaa gagacccgcc gcccgggtgg
     1921 gcgacgggtc tcagcacgga tcgcgtcgag gtcctacttc gccagcgctg agcacaccgt
     1981 gttggtgatc agcgcggaga ccatgtacgg atcggcgttg gcattggggc gacggtcctc
     2041 gatgtagccc ttcttgtcga cgcccacctg ccaggggata cgcaccgaag cgccccggtc
     2101 ggagaccccg tagcggtatt cgctgaagtg ggcggtctcg tgctggccgg tcagacggtc
     2161 ctcgtagccg tgtccgtaac cggcgagatg ctcctccacc ttgcctggcg cgccgagcga
     2221 ctcacaggct gcgatgatcg gatcccagcc ttcgcgcatc gctgcggtcg agaagttcgt
     2281 gtgcatgccg gcgccgttcc aatcgccctt gatcggcttg gccgagatcg atgcattcac
     2341 gccgaagtcc tcgccgatcc ggtagaggag gtaacgggcc atccaaatgt ggtcggcgac
     2401 ctcaagggtt cctgcgatac cgatctggaa ctcccattgg ccgagcatca cctcggcgtt
     2461 ggtcccgcag atcttgaggc cggcagcgat acaggcctca gtgtgggcct cgacaaagtc
     2521 gcggccggcg atctccaccg atccgacacc gcagtagtag gggccctgcg gggccgggta
     2581 gtagtagccc tccttgggcc acccgagcgg gcgaccttcc tggaacagcg tgtattcctg
     2641 ttccatcccg aacatcggag cctgatctgc aaacttggcg tcgacctcgg cgcagtagcg
     2701 gcgggtgttg gtgctgtggg gctccatcgt cccgggcaac aacacctcac agagcacaag
     2761 gatgtctccc ggaccgcggg tcgggtcggg gcaggtgaac actgggttca gcacacagtc
     2821 ggagtcgctg cccgcggcct gattcgtcga agatccgtcg aatccccaga tccccggctc
     2881 accgccgtct tcgatgatct tggtcttgga tcgcatgagc ggggtcggct cggttccgtc
     2941 gatccagatg tattcggcac gatatggcac ggggcaccat cctgagtttg tggccgactc
     3001 ggtcgaccgg gggttgttga aacgctcgtt tcgagcagcg atcggttcgg ggcactcctc
     3061 gaagcgatgg gagccacatt gcacctccgc gatttctcgc ccttttcccg gttgtaaacg
     3121 ctgtgttaac tcggcgcgtt gcgttgggat ttcttggccc gcatcggcga aggtagaggc
     3181 atgggacatc accacgacta cgtgttgcgc acactcgagg agcgaaaggt ccacctggtt
     3241 cgcctctggt tctgcgatgt gctcgggcag ttgaagtccg tcgccatctc cccggtggag
     3301 ctcgagactg cgttcgaaga cgggattcag ttcgacggat cggccgtcga cggattcagc
     3361 agggtccagg aaagcgacgt gttggccatg cccgacccgt cgagtttcca gctcatgacc
     3421 tggggcgacg gcgaggtgag tgctcgcatg ttctgcgaca tcgtccacct cgacggcact
     3481 ccgttcgagg gcgaccctcg acaggtactc aagcgcaatc tccagcgtgc ccgcgatcgt
     3541 ggcttcgagc tgatgtgtgc ccccgaggtc gagtacttct acctcgcgtc cgacgacccg
     3601 tcgcaacctc cacagaccct cgacaacggc agctacttcg atctcaccac cagcgacatc
     3661 gcctccgaca tccgccgggc gacgatacac cagctcgaaa acacgggcat cccggtcgaa
     3721 tactcattcc acgaggacgc gccgtcgcaa cacgagatcg atctgcgcta cgacgacgcc
     3781 ctcgccatgg ccgatgccat catgacgttg cgcgtggtgg tgaaggaggt cgcctaccaa
     3841 cacggcgttc acgccacgtt catgcccaag ccgatcgcgg gtgtccaggg ttcggggatg
     3901 cacacacaca tctcgttgtt tcgcgacgag gagaacgcct tctacgactc cgagggcgat
     3961 gcgaacctgt ccgaggtcgc ccgtcagttc atcgccggtc tcctgcgtca cgctcccgag
     4021 atcactgcgg tcaccaacca actcgtgaac tcctacaagc ggctgatcgg cggcttcgag
     4081 gccccggtgc acgtgagttg ggcccgcaac aaccgctcgg ccctggttcg ggtaccggtg
     4141 tccaaaccga acaaggaggc cgctggcacg cgaatcgagt accgagcgct cgaccccgcg
     4201 tgcaacccgt acctggcatt ctccgtgctt ttggccgccg gtctcgccgg tatcgaaggc
     4261 aactatgagt tggcgccgga aaccaccggg aacctcttcg aactcgccga acagggcaac
     4321 ctcgatgagt cgattgaact cctgccgtcc tcgctcgccg aggcgctcga cctgatggag
     4381 cagtccgaac tcgctcgtga agcactcggt gagcacatct tcgagtggtt cctgcgcaac
     4441 aagcgcgccg agtgggcggc ctataaggca cacgtcagcc aattcgaact cgaccgttac
     4501 ctcggtaact ggtaggaggc accgtcgtgg agccattgct ctgttggccg gatcccgttc
     4561 cgcccgaaat cgtcagatgc ctggagctgg cgtcgatccc gtggggaacc gccaccaccg
     4621 atacggacat cgccgccgcg acgtgggcgg gagcgatcgt cgatgcgtcg aacgacaccg
     4681 atgaggcgtt cacctacgca cgtcgattgt cagccacgaa cctcggcgtc gcaccgctgc
     4741 tgttgctggt tcgaggctca caactccccg acctcgatcg tcatcacgac ctgtttgagg
     4801 acttctgcgt cgcaccgttt cacccagtcg agttcgaggc tcgagtgaag cacctactga
     4861 cccgccaggg actactgagc gacgacagcg agatcatcca gtacaagtcg ctcgtgttga
     4921 acctcgagac ctaccaggcc tcgatcgacg ggacaccgct cgatctcacc tacatggaat
     4981 atgagctgtt gaagttcctc gcccagaatc cggggcgggt gttcacccgc gagacgttgc
     5041 tgagccgggt gtggggctat gagtactacg gcggcgcccg cacggtggat gtccatatcc
     5101 gccgcctccg cgccaagctc ggcgaacaac acgctcagat gatcggaacg gtcagaagcg
     5161 tgggctaccg gtttggcaag tcccgttggg actcatagac acagcccgtt gggactcatg
     5221 acagctagcc gttgaggtcg atgagttcgg aaccctcgtt cgtgtcgtcg acgtcctttt
     5281 gcacaaagaa accgatgacg ccggcgagcg cgccgcccga taccaggaca ctcacgggga
     5341 tcacgacgag cagcacgatc acgatgatga tggctccaac catggccaca ttgtggccga
     5401 agtaacccac gtggaccaat accgacgcgg tccaccgaac gttctggccg gcggggccga
     5461 cgatcagccg atgtggatga ccgcctcgat ctccaccgcg gcaccgagcg gcaacgctgc
     5521 cacagcgacg gctgaccggg caggccggtg atcgccgaaa ccggccacgt acgcctcgtt
     5581 catcgtgagg aagtcatcca tgtccgcgag gaacaccgtg gtcttcacca cctgatccaa
     5641 cgttgccccc tccaattcga cgagcgctgc caggtttcgc agcgcctgcg cagtctgggc
     5701 tgcgacgccc tcgacgagcg cgccgtcgag gagtccgagt tggccgctcg catacaggaa
     5761 cgatcccgct cgaacgatgg gtgtgtaggg accaaccggc gtgctcatgg ctcaaggcta
     5821 ggccgtggct ggatccgaaa atcggaccgg gagcttcgtc ggccagacac ccggggtgcc
     5881 ctgcaacgtc cagatcgccg cagcggtcgc agcgaacacc gcgtccgacc ctgacaccgg
     5941 ctcgatgccg ggttcgtcca ccaccgtcac ctcgaccgga atcatgtcca cgggacgcac
     6001 cacaccgaag ctgcggatcg tgagattcaa cggattgccc tggtcatcca ccgacaactg
     6061 ctccgacgtc acgagaccca accccatatg cactgcgccg atggcatatg cacgtacgac
     6121 ggaggtgtcg agcacctcgc cgcagcgcac gacgacgtgc gcaccctcgt cgtcgatgcg
     6181 aaccgtcgcc gacgcaccct cgggagacct cacggttgcg gtgccgggct ggtccgtggc
     6241 cagggattcc agcgaggcga gcacggctgc gacctcggcc cagccggccc ctcgaatcgc
     6301 cgtcgaggtc ggcggtccga cgatgtcgac ctcctcgacc tcgatcagtg gggctaccga
     6361 gacgatggct tcgacgattc cgggcgtgcg agcgacccgc accacaccgg aaccatccga
     6421 gcgcatgccc agcgccatcg gcggtcgctt cgggccacga cgcaccacgt cctcgcgcga
     6481 ccagcgcaac cgcacgggac caccttcgcg agccgccagc tcgacagcgt caccaggcac
     6541 cggcgaatcg accttgccac cgaaggcccc accattcgcc aacggatcgg tcggatccga
     6601 acccggaatc gcccaggccg cgtcggtctc gagatacgcg ggttcgaccc agttcgtctg
     6661 cagggtgagc gcccagtccc cgtcgggcac ctcgatcggc gcaacggcat cgaccgtcgt
     6721 gttgcgtccc tggattttgc cgatcgcggc gcgggcctcg gtcatcgtcg gggcgaccgt
     6781 ccaggttcca tccgcggcga gcagcgcgac gggcgcatcg ctcggtgcgc cgtcatcggc
     6841 aaacccaccc atgcccaagc cgatatcggg cccgacgcgc tggtgcgcgt gcccctcgat
     6901 cgttgcgcgc cgcgacgctg cgtcgaagtc acgactctct gatcgcacct ccgcgccggt
     6961 agcgaccatc acggccgcct cgcggatcgt ctgccagccc gtgcaacggc acaggtgggc
     7021 agcgagggcg cgatcgacaa cggcgcgttc gcacagatcg ccacccttgc ctgcggtccc
     7081 ttcgaggcgc atcacgatgc ccggggtgca atagccgcat tgactgcccc cggtcgcgca
     7141 cagcgcctca ccccaggcct cgcggcgctc gtcgctcaac ccctcaaggg tcaccacgga
     7201 ccggcccgca acgcggcgcg ccggggtcac acaaccgaca cgagcctcac cgtcgacaag
     7261 cacggtgcaa catccacatt gaccctgcgg gttgcatcct ggtttgaccg aggtgacacc
     7321 gagacgatct cgaagcacct ccagcaggct ggcgccgtca tcggcgacct caacctgttc
     7381 gccgttgagt tcgaattgca acgagaacgc tcagcgactc agctgaacga tgagccgcag
     7441 ccgcaggtct tcgaggcgtt cgggttgttg atctcaaacc cggacttctc gagaccgtcc
     7501 ttgtagtcaa gctccgcgcc gacgagcagg ggcaggctcg tcgggtcgac cacgaccttc
     7561 acgccgtcga agtcgttcgt ctggtcttcg gtcttgatct cgttgtcgaa gtacatctca
     7621 taggagaagc ccgaacaccc accgggacga acggcaacac gcaatgcgag cgactcgtcg
     7681 ccctcctggt cgatgagctg tcgaaccttg gttgcggctg aatcggtgag cgtgatcatt
     7741 cgtgcgcctc ctgcaaagcg gtgccgtgac gaggggtgaa gccagtgtaa ccgagcgccg
     7801 aaatgggacc tcgggcactt ccgcaaacac cgtttcggcg ctacatattc ccggttcggc
     7861 cgcagatttt ccagtccccg ggtcgtaaac tcgtgaccga tgggcattcc ctccgactcg
     7921 ccccgcccga gcacctccga gatcaccgga ctgttacgag gcgtcatcga ccccgagttg
     7981 gggtccgaca tcgtcgcgct cggcatggtc aaagacgtgc gcgtcggcga cgacggactc
     8041 gtcgttgtga agatcgcgct caccaccgca ggctgtccgc tgcgtgccca gattcagcgc
     8101 gatgtccgcg atcgggtcct gtcgttgccc ggtgtcacca aggtgaaact cgattggacc
     8161 gaactcgacg ccgatgagaa ggccgcggcg atggccgtcg cccggcgcaa catcgccgag
     8221 catccgccag aaacagcggt tccctccacg accaaagtgc tcatggtggc gtcgggcaag
     8281 ggcggcgtcg gcaagtcggc ggtgacctcg aatctggcaa cagcgatcgc cgccctcggc
     8341 taccgcgtcg gcgtcatcga tgccgacatc tggggcttca gccaaccgcg gatgctcggc
     8401 gttcacggcc gccttgaggc gcacgagcgg accgcgggca gcgtcggcgg atccgacgct
     8461 tccgacgcca ccatcgaacc gaggatccgc accgtcggcg acggagaact acgagtggtg
     8521 tcgatggggt tcctcgtaca gaacgaggaa gacgcgctca tgtggcgtgg gctcatcttg
     8581 cagcgagccg tccagcactt cctcgaagac gtcgcttggg gatccgacct cgactacctg
     8641 ctcatcgaca tgccccccgg aaccggcgat gtccagatgg gcctcgcgaa actcctgccg
     8701 agggccgaga tgctcgtggt caccacgccc tcgctgtccg cccagaaggt ggcggcacga
     8761 gccgtctcga tgggccgcaa gaattacctg cgggtactcg gcatcatcga aaacatgagc
     8821 tatttcgtcg caccgtccgg cgaacggttc ccgatcttcg ggtctggtgg tggcgaccgc
     8881 ctggccgcca ccacgggggc gccgctgctg acacggatcc ccatcgaaaa tgcggtggcc
     8941 gagggcggcg acaccggctc accgatcgcg ctcggcgacg ggcccgccgc cgaggccttc
     9001 cgcgatctcg ctcggcgcat caccaccgac atcgcgcccc caaccgatct gactggatgc
     9061 tcggcccgcc tgctcgacgc ggtcgccgcg gcgctcgatg cccacgacag cgccgaacga
     9121 gaccgttgcg acgactcggt caattcagac gtttcgagct gaacgcgtcg tagacctcgt
     9181 cccccggcag aacgatccgc agtcccccgc cgtcctcgac gatccggggc aggatggctg
     9241 ggaactcggc gatcgaacgg gcgtgagcca ccacggcctc agccgaatcg aagcgggcca
     9301 gcacttcgag ggtacgaacc gtcgggaaga tcaacgtgaa atcccccgcc tcgttgcgct
     9361 cgagggcctc ggtcggccgg atccacaggt tcgcgatcgt ctcgcggtcg tcgtgagatg
     9421 gcacctgacc ctcgggtgcg tgggccacga agaagcgggt gtcgtagcgc cgaggtgaac
     9481 ccagcggcgt gatccagcga ctgaagtagt gcatccgatc ggtggcgaga cgcagccgtt
     9541 cctcgcgaac gacatctcgc agggcacgaa ccgccgtgtc gacatcgatc cggtgacgat
     9601 gaaatcgacc ggccacctcc gggttggcga acccgaccat gcggtcgtcc tcgtcgaccg
     9661 ccaataacag tccggcctcc tcgaacgact cccgaatcgc tgcaacccag taggcaagcc
     9721 ctccgcgacc cacaccgagc aactctgacg cctgagcgtc ggacagtcca acgctcaggg
     9781 cctcgatcgc acggtcgcta tcgcccgggt cgaccgcccc gccgggaaag acatacgcgc
     9841 caccgacgaa atcggagttg agattgcgtc ggagcatgaa cacttcgaca ccgtggcgtc
     9901 cgtcgcgaac cagcatcacc gtggccgcgt cgcgaatcgg gatctcgtgt gggtcgtcga
     9961 cctggaggct caacgacatt gcgtcaggtt cccgggccga gttcgccgcg gggctcgtcg
    10021 aggaagatct cctcggcgtc caggtcgatg atgccgtcat cgtgtcgcgg ccgccgacgc
    10081 aggaagtcgt cgtcgaaacc gccccgggcc gtggagaacc cgaaggttgc gaaccgcacc
    10141 gcagaaccgt tgaaggtcga ctcagcacgc acctgggcac gtcgaacaac gagcggtcgc
    10201 agcagggccc gcgtagatgg gagcaccaac acgagcgccg cgaggtccga cacgaatccc
    10261 ggcaccagca acagtgcacc ggcgaccatc cagatcaatc cgtcgagcac ggcgtcggtc
    10321 ggcatttcac cccgagcaaa cccgtcattg atccgtcgcc acgtcgcgaa cccctgacgc
    10381 ttcacgaccc acgccccagc ggcgcacagc agcgtcagcg ccaggaacat ggtgatgacc
    10441 gacgttgcgt tcgccaccgc gatcatcacg gtgacttcga cgacgaacag cgccagcagc
    10501 gccaggagca cgaaagtcac gatccgatgg taccccgctg gattccgtga cttccgtgca
    10561 acccggtgcc gagaacgatg aacaccctca ggcgctgcgg acctcgagca tcgctcgacg
    10621 ctcgacctcg ttggttccgc cccagattcc gtgtggttca cggatctcca aggcgaagct
    10681 cagacagtca ctcaccaccg ggcactgagc acagatctcc tttgcccggt gctcccgggc
    10741 gattcgatcg tgcttgcgtt cgaagtgcgg cggcggaaag aagaccgctg actgtggtcc
    10801 gcggcatgcc ccccggtgat gccatgaatc aaaagatggt tgtgcactca acgttgttcc
    10861 cccttttcaa cggcgcagga tacgccgccg ggattatgca aacaaggttg agaaaagagt
    10921 ttctgtgaat caggtcacag aatcgggctc gtcaccgggg tcatcggaag ctgctggccg
    10981 ccgatcgcgg tagtcgatcg cgccgccttc gggaggttct atttccagcg tcatcccgtc
    11041 gatgccaacc acccgaagct cccctccggc catgaccggg gtcgcacggt tggtgatcgc
    11101 tcgccagatg ccaccgtcga cgtcgacggt gccttccggg ttgatgtcgc tgatcgcggt
    11161 ccccatcttg ccgatcatcc agcttcggcc caacgtcggg gtcgcaaagc gagaccgcac
    11221 catcgacggc atccccgaca gcatcagcac cgccatgcca cccaggccgg tcaacagcgc
    11281 caaccacggt gcacggagac cgtcgaacag gaacagcgag ccgaccgacc agctgaccag
    11341 cccgatcaac gtccacagcc gaggtatcgc tgtttggatg tcgatggcga acgcgaacgc
    11401 cgagaacagg cacagagcca gggcccacca gcgaagcggc aactcggcaa gcccatagcc
    11461 cgccatcacc gcacagatcg cgccgacgac gccggccaca ccgacgccgg cggtgaagaa
    11521 ctcgaacagc agcaggccga tcgcgatggc gagagcaagg taggcgaccg ccgggctcgc
    11581 cgctgtgtgc agcagttggg tcgtgaccgg caggccgatc gtgatgttgg tggtgagcag
    11641 ttcgcgcttg gggcggccct catcatcggt cacgtccttg aacgcaacgt cgcctcggtt
    11701 gagcgcgtgg tcgcccaaga tggcgtcggt gcgcaacgac aatgagcgct gttcggcctc
    11761 gtcgttgtcg atcaccgttt cgcgaaggcc ggtgtcggtc gacgtgacca gatcgccgaa
    11821 ccgctgctgg tcgagctgtg gcacgccgac gtcgccgatc ctcgtgttgg gggccatcga
    11881 cggttcgagc gcggcgacga gttcggcggc gccgccgagc gcttgtgccc ccgacggacc
    11941 gacccatgcg gtgaccttca acggaaggtc gaccaggtcg acgaccagat cgttcagttc
    12001 ttcagcgctg atgaccgacc cttcgctgtc gatgaccagg atgatcgccg aatatccggg
    12061 cacatcggcg acctccgacg cggcccgacg aatgaaatca gcgttgatct gatcgatgaa
    12121 gccgttcacc gtgatggccc gcacgcaccc gttttcattc gggcatggag ccaccgcggc
    12181 cgaggccgga cgggcggacg cagcaacgcc aagcgaggaa aagagcgcga gcaataccgc
    12241 gaagatggtt cggctgagta ctgtgcgcac ccgtaggcct ccggaggaaa agtggatcct
    12301 gcgcagtttt tcactgcacc acgactgacc atcgttgccg ggaagggcgg ggtgggcaag
    12361 acgaccgtca ccgccaccct ggcgctcgcc gccgcgcggg cagggttgcg ggtgctcgtc
    12421 gtcgagatcg aggggaagtt cggtctgccg gcgctgttcg acaagccggc gctttcctac
    12481 gaggaactcg tgttgttcga ggacctgcac ggagccggat cggtgcaggc gcggaccctc
    12541 acccccgacg ccgcgctgtt ggagtttctc ggcgatcacg ggatgcggcg actctcgagg
    12601 cggctcgccg attccggagc ggtcgacatc gtcgccacgg caacccccgg gatcaaggac
    12661 gtgctcgtgc tgggcaaggt caaacaactc gaacgatcgc ggacctacga cgcgatcatc
    12721 gtcgacgctc cggcggccgg acacgccatc acgttcctgt cgagcgccaa gggcctcctc
    12781 gatgccgttc acgtgggccc catcgaatcc caggcccgcg aggtgaacga catgctcacc
    12841 gacgagaccc gttgtcaggt ggtgctggtg accctgccgg aggagacgcc agtcaacgaa
    12901 ctcgtcgaaa ccgcctacaa cctcgaggat cgaatcggca tcaaactcgg ccccgtggtg
    12961 atcaacgggg attacgagca cctcgacggc atcgaggtgg ctcccggcga cgccgctgcc
    13021 gccgcgggcg tcaccctcga cgcccggctc agcgccgacc tcgctgcggc gtccgcactt
    13081 cgacgtgaac gcacagcggc ccaggtgtca caaatcgagc ggctcaatcg agagctgccg
    13141 ctgccacagc ttcggctgcc gttcgtcttt cgcaccgacc tcgatctcga tgcgctcatc
    13201 acgctgtccg attcggtact cgatcaagtg agggcgatat gaccgcgggc gggccgacac
    13261 cgcaggagtc gttggagcga ttgtgcgccg actccgacgt gatcctgtgc tgtgggtccg
    13321 gtggtgtcgg caagaccac
//
LOCUS       DAOOMA010000009        13166 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_5206_length_13166_cov_4.758371, whole genome shotgun sequence.
ACCESSION   DAOOMA010000009 DAOOMA010000000
VERSION     DAOOMA010000009.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 13166)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13166)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13166
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5206_length_13166_cov_4.758371"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..731
                     /gene="folP"
                     /locus_tag="PLP95_00565"
     CDS             <1..731
                     /gene="folP"
                     /locus_tag="PLP95_00565"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR01496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HPB44327.1"
                     /translation="VDEGADVIDVGGESTRPGAVAVSIDEELERVVPVIAAISGFGAR
                     ISVDTRHEQVASAAIDAGASIINDVSASLWPVAARRGVGWLAMHMAGDPASMQQRVPT
                     YRDVCREVGDELVAHANAAAHGGVAEIWIDPGIGFGKTAQHNLELVARIDELVATGIP
                     VALGVSRKSTLGALCAQADRTPDRPPAPTDRLEAALTMAVWAMAAGVSLIRVHDVAAH
                     VAALGMAFERLNAPNHLQNGLRAG"
     gene            769..1314
                     /locus_tag="PLP95_00570"
     CDS             769..1314
                     /locus_tag="PLP95_00570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB44328.1"
                     /translation="MAAKGKWAQGIEPRHFHWIMQDKLGVCERPGGYGQNHRKVRRQE
                     EIIWIRETGFNPVISLIPASHNLHNYDELGVPWRHRPFPAAEDYPRYLSEVYPELATA
                     LASGAKVLYHHEDLNDKVLGLMAGYLVWAGLVAEQPRAIFLVEHITSRQLGPQARDLV
                     TVAAKVRDEMLARARSASGAE"
     gene            1311..1688
                     /gene="folB"
                     /locus_tag="PLP95_00575"
     CDS             1311..1688
                     /gene="folB"
                     /locus_tag="PLP95_00575"
                     /EC_number="4.1.2.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HPB44329.1"
                     /translation="MTVAMTDVIELRGLRALGYIGALAEEQQRRQPFEVDLDVEVDLS
                     QSGHSDDLDDTVDYGALCATVEGIIEHERAALMERAAARIAHEVLLDRRIDAVTVSIR
                     KLRPPVPQHLETSGVRIRREQRR"
     gene            1685..2140
                     /gene="folK"
                     /locus_tag="PLP95_00580"
     CDS             1685..2140
                     /gene="folK"
                     /locus_tag="PLP95_00580"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HPB44330.1"
                     /translation="MTRSILALGSNLGDRRAYLSDAIASLPDLVAVSNVYETEPVGGP
                     LRQGPYLNMVAVLDTDLSPKDLLGLCHRLEAAAYRVREEHWGPRTLDVDIIWVDGVEM
                     DDPNLTLPHPRWKDRRFVLVPMQEVAPDLVSDRDIETADGRVWLAEGLA"
     gene            2137..2880
                     /locus_tag="PLP95_00585"
     CDS             2137..2880
                     /locus_tag="PLP95_00585"
                     /inference="COORDINATES: protein motif:HMM:NF022188.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2520 domain-containing protein"
                     /protein_id="HPB44331.1"
                     /translation="MTTKVRIVGAGRAGGAFALALRRAGWNVDGPIGRDHAAIEAAAD
                     GVDLVLVCVSDPAIAEVASTIRPGDAVVAHVAGAVGLDVLAPHRRVGAIHPLVSLPSA
                     ELGAERLVGAWYGTGGDPIVWEVARALRGRSFRISDLDRVTYHAAACVAANHLVALLG
                     QVERLAASIGAPIDAYLDMAAAVVENVRTLGAAEALTGPAARGDLATIERHLEAIGPD
                     ERDTYRVLAREATRLAGRDLLAPLDRIEG"
     gene            2884..3732
                     /gene="panC"
                     /locus_tag="PLP95_00590"
     CDS             2884..3732
                     /gene="panC"
                     /locus_tag="PLP95_00590"
                     /inference="COORDINATES: protein motif:HMM:TIGR00018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HPB44332.1"
                     /translation="MEIIEGIAELREALDDHRANGRRVGFAPTMGYLHDGHESLFKRA
                     VGDCDVAVASIFVNPLQFGVGEDLDAYPRDPHGDRERAQRCGIAYLFTPSVEEMYPRP
                     ILTTVSVRSLAAVMEGASRPTHFDGVATVVTKLLSIVGPCRAYFGEKDYQQLAIIRQL
                     AFDLSLPVEIVGCPTIREDDGLARSSRNVYLSDAERAVAPVLHRALRHGADLIVGGQR
                     DAEVVRRAMAEVIEAEPLGELDYVEVADGATLEPQLRCDLSSRLFGAVRFGRARLIDN
                     VGIEGR"
     gene            3729..5324
                     /gene="nadB"
                     /locus_tag="PLP95_00595"
     CDS             3729..5324
                     /gene="nadB"
                     /locus_tag="PLP95_00595"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR00551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008734 - L-aspartate oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HPB44333.1"
                     /translation="MSTQSSEELDLLVVGSGVAGLSAAVRAAEVEGLSVGVVTKAELD
                     LATTRWAQGGVAAALSGEQEETDLHLADTLAAGVGLCDIEAVRVLVDEGPGRVHDLIA
                     LGALFDRDEHGELELAREGGHSVPRVVHAGGAATGAEIERALVVAVRERVREVHEFTF
                     ALDLIVEDGRCCGVTAARDGRVFEVRARNVLIATGGAGQLFAVTTNPPVATGDGVAMA
                     LRAGVAVADLEFFQFHPTALDHPAMPRALLSEALRGHGALLRDSRGERFVDELLPRDV
                     VSRAITAKMLEEGSDRVWLDATVLKDFDVRFPTILADLESAGLDPAHDYLPVAPAAHH
                     QCGGIITDLDGATSLPGLWAAGETSCSGVHGANRLASNSLLEGMVFGARVTEAISRGQ
                     RGPCDTGAMRSVNREGSTDRVIGGRRITVAALETSTATDLGRDAVDELRRQVQRTMTI
                     HAGVLRTAESIDTARNDLAEVWAQLPAHSTDPAVCELINLAVVGVALLGAAQAREESR
                     GAHTRTDFPGTHEEFRVRLVIGS"
     gene            5324..6199
                     /gene="nadC"
                     /locus_tag="PLP95_00600"
     CDS             5324..6199
                     /gene="nadC"
                     /locus_tag="PLP95_00600"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR00078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HPB44334.1"
                     /translation="MNTYFDPPLVLVRDAVRRALEEDLHPLGDLTADLLDPEARTVAR
                     FNAREAGRLAGTVCAQEAFAQVDDSIEVEWVKRDGDTLAPGDDIGFVRGRLAPVLTAE
                     RTALNFLGHLSGIATLTNRYVVAAEAGGGAKVWDTRKTTPGLRAIEKAAVRAGGGRNH
                     RGNLSDWMLLKDNHITGIGITEAVTRCLDLWPARTVHVECDRLDQMVEAISAGAHAVL
                     LDNMSPDQVRECVAAASELADRGFRALIEVSGGVTLDTIGEYSGTGVDQISIGRITNS
                     APVLDIGLDIGPPVA"
     gene            6287..7063
                     /locus_tag="PLP95_00605"
     CDS             6287..7063
                     /locus_tag="PLP95_00605"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012223741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HPB44335.1"
                     /translation="MLLAIDVDNTQTVIGLYKRGEESHELLDHWRIATNATRTCDELA
                     LLFSEFLLSAGHDWRQDVSGVAVSSGVPDVTFELRRMCERYLDIDPVVLGAGVKTGMP
                     VLYDNPKEVGADRIANAVGAYDLYGGPTIVVDFGTATTFDVISAAGEYLGGAIIPGIE
                     ISLDALFGRAAALRRVELTEPRSVIGKTTMESIQSGVLYGWASMIDGMSDRIEEVVGD
                     ATVIATGGLADLVAPLSDAIEHVEPWLTLHGLRLIWEKNR"
     gene            7060..8589
                     /gene="lysS"
                     /locus_tag="PLP95_00610"
     CDS             7060..8589
                     /gene="lysS"
                     /locus_tag="PLP95_00610"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: protein motif:HMM:NF001756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HPB44336.1"
                     /translation="MTTEPNHTTADSPPSAETPALPHRAEVTDTAAALADRFAGLEPG
                     TETGVRAAIAGRLMLRRVQGKLAFGTLADQTGRIQLFAPSQTTPDFERFTALSLGDWI
                     SVTGEVMTTKRGELSVRVDDWTLLAPTRRSFPDKWHGITDTDMRYRQRYVDLWVTDEA
                     RAAFTNRSRILSLTRRFMEDRGYLEVETPILHPLAGGALARPFATHHNALDSELFLRI
                     APELYLKRLVVGGFTKVFEIGRVFRNEGMSTRHNPEFTMLECYEAYGDYHVHMDLTEQ
                     LIAHLATQICGSTVVTYGDRELDLSAPWRRAPLATLLSEQIGVEVDVHTPVGELRRLC
                     DEHETPWKESYGAGKLLLELYEKTVEHTLWEPTFVIDYPKEVSPLSRVHRDDELLTER
                     FEGIVAGRELCNGFSELCDPDDQRERFEEQAAQKADGDDEAMVVDEDYLRALDYGLPP
                     TVGLGIGMDRLVMLLTNTTTIRDVVLFPTLRPESPDVPVADVGPSSDGPSNDGAADAG
                     S"
     gene            8625..9734
                     /locus_tag="PLP95_00615"
     CDS             8625..9734
                     /locus_tag="PLP95_00615"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPB44337.1"
                     /translation="MSKSFGSNMVLDECTFDVAPAEIVALLGPSGCGKSTLLRIIAGL
                     ERLDGGVVELASAHGAQRVVAGSDPASGKAVHVAPERRGVGLVFQNGALFAHLDVAHN
                     IAFGLAKGEDRTSRVSELLAMVDLAGFEDRYPDQLSGGQQQRVALARALAPRPAVVLL
                     DEPFSNLDARLRRSLRREVTEVLRRSATPTVIVTHDRDEAFAVADRVAVMRDGRIAQF
                     GTPSDVYDRPVDDWVARFVGDANFLEVRTVGDREVDTLADTVVGPVRVGAPVAAGDTV
                     MIRPECLTFERTSAWPLGRVVGVEYTGATLLVDVDVDVGAEVDAKADAGAVVTHGSVR
                     LRSEQSTAGAAPLRIDDRVVVHVHPATVAHRVEVS"
     gene            9809..11029
                     /locus_tag="PLP95_00620"
     CDS             9809..11029
                     /locus_tag="PLP95_00620"
                     /inference="COORDINATES: protein motif:HMM:NF014691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HPB44338.1"
                     /translation="MITDPALPIEPVRLEDHEWIAESDGMSSRARVAAGSGPYQSVVP
                     ATIASYRPTVPSDLTADIEEASAALARFDSYTQAVLGIEHPALGPMSSILLRTESASS
                     SQIENLTVGARQLALAEIAQPSSTNAKIVAANVRAMEAALVLADNLDEAAVLAMHAEL
                     ISRQPGWEQHAGCYRNSLVWVGTSGISPRGATHVAPQPELVSSAMDDLQQFLARDDLP
                     VLLQAAVAHAQFETIHPFADGNGRTGRALVHAILRGKGLMTHTTAPVSAGLLTDTESY
                     FGALSTFRAGDARPIIEEFARASRYAATTGTRLVDDLAAQVERARVQMAGLRPQASAW
                     RVLPHLVAQPVINARYLVDHLGMSDMTAQRALGQLTAAGVLVERTGHRRNRIWQHSGI
                     LGVLDGYAQQLRRS"
     gene            complement(11145..12179)
                     /locus_tag="PLP95_00625"
     CDS             complement(11145..12179)
                     /locus_tag="PLP95_00625"
                     /inference="COORDINATES: protein motif:HMM:NF012596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HPB44339.1"
                     /translation="MEVRDLRRLIALESSSLLDDASILHASEPLVLVPTGDESERDLA
                     AIAAVASVAPTVTVLVDETGRLADRRRFDRLAKATDISLTTAPDPPSPWIHDELKSIT
                     EPVAAHPLAAFSLATLLRSSFVRPVWEGLAAEATSYSMLLGAQDFRGWLQRRGPATVK
                     DHVGRGVVIRKHGPILQLKLNRAEARNAIDSSMRDLLVDAFRNARIDPSVARITLTGN
                     GPSFCAGGDLAEFGTAPSGATAMAARLTRHPGWEAHQVADILTVHLHGHCVGAGIEIA
                     AFARQVEAAADTVIQLPELSLGLIPGAGGTVSIPRRIGRQRTMWLALTQTRLTATEAL
                     HLGLIDRIVD"
     gene            12219..>13166
                     /locus_tag="PLP95_00630"
     CDS             12219..>13166
                     /locus_tag="PLP95_00630"
                     /inference="COORDINATES: protein motif:HMM:NF014564.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HPB44340.1"
                     /translation="MGDSMVGLTPEAEWARSGVVTLTGDVEGPALLPPGAAAAMVEAE
                     AAAFGLDARVLSERAALMGLHRNGRTSCGGASCLLEVCDGWVVLSLARGSDIELVPAL
                     IGGEKVPSGSPWDAVVEFARTVSAAEFVERAAVLDLPGAVVDASPAPSDVEPGEFTVS
                     RRDSARSRPVAGAGAAPDGPLVVDLSALWAGPLCSHLLQRRGARVIRVESVSRPDGAR
                     RGNRSFFDLLNAGKQSVAFDFDSPEDRRRLRGLIDAADVVISSARRRAIEGLGLDVGA
                     FLAGGTDRVWVAITGFGWNSNRVAFGDDAAAAAGLVAWGD"
BASE COUNT         2076 a         4154 c         4688 g         2248 t
ORIGIN      
        1 tggtcgatga gggcgccgac gtgatcgatg tcggcggtga atccacccga ccaggagcgg
       61 tcgccgtgtc gatcgacgag gaactcgagc gggtcgttcc ggtgatcgcc gcgatcagcg
      121 gcttcggggc gcggatctcg gtcgataccc gacacgaaca ggtcgccagc gcggcgatcg
      181 atgccggtgc ctcgatcatc aacgatgtgt cggcgtcgct gtggcccgtc gcggcccggc
      241 gcggggtcgg ctggctggcg atgcacatgg cgggggatcc ggcctcgatg caacagcgtg
      301 ttccgacgta ccgcgacgtg tgccgagagg tgggcgacga gttggtggcg catgccaacg
      361 cagcggcgca tggcggggtg gcggagatct ggatcgatcc gggcatcggg ttcggcaaga
      421 cggcgcaaca caacctcgaa ctcgtcgcgc gaatcgacga gttggtcgcg accgggattc
      481 cggtcgcgtt gggggtcagc cgcaagtcga ccctcggcgc gttgtgcgct caagcggacc
      541 gaaccccgga ccgccctccg gcgccgacgg accgtctcga ggcggcgctg acgatggcgg
      601 tgtgggcgat ggccgcgggc gtgagcctga ttcgggtaca cgatgttgca gcgcacgtcg
      661 ccgcgctcgg gatggcgttc gagcgcttga atgcccccaa ccatctccag aacggcttac
      721 gggccggctg attcgacgaa ctccaccagg attggaaaag gacagatcat ggcagcgaag
      781 ggaaagtggg cacaggggat cgaaccccgg catttccatt ggatcatgca ggacaaactc
      841 ggcgtgtgtg aacgaccggg tggatacggt cagaatcacc gcaaggtgcg ccgccaggaa
      901 gagatcatct ggattcgcga gaccggcttc aatccggtga tcagcctcat cccggcgagt
      961 cacaacctgc acaactacga cgaattgggt gtgccctggc gtcaccgccc gttcccggcg
     1021 gccgaggact atccgcgcta tctcagcgag gtgtacccgg aactcgccac agcgctcgcg
     1081 tcgggcgcga aggtgctgta tcaccacgag gacctcaacg acaaggtcct cgggctcatg
     1141 gccgggtacc tggtgtgggc ggggttggtc gccgagcaac ctcgcgccat cttcctcgtc
     1201 gaacacatca cgagtcgtca gctcgggcct caggcccgcg atctggtgac cgtggcggcg
     1261 aaggtccgcg atgagatgtt ggcccgggcc cgaagcgcga gcggcgccga gtgaccgttg
     1321 cgatgacgga tgtgatcgag ctgcgcggcc tgcgggccct cggctacatc ggagcgttgg
     1381 ccgaggaaca acagcgtcgc cagcccttcg aggtggacct cgacgtcgag gtcgatctct
     1441 cgcagtcggg ccacagcgat gatctcgacg acacggtcga ctacggcgcc ctgtgtgcca
     1501 cggtcgaagg catcatcgaa catgaacgcg cggcgctgat ggagcgagcg gcggcacgca
     1561 ttgctcacga ggtcctgctc gatcggcgca tcgacgcggt cacggtgtcg attcggaaac
     1621 tgagaccgcc ggttccccag cacctcgaaa cgtcgggagt gcgaatccgt cgagagcagc
     1681 ggcgatgacc cgttcgattc tggcgctcgg atcgaacctc ggcgaccggc gggcgtatct
     1741 gagcgatgcc atcgcgtcgt tgcccgacct ggtggcggtt tccaacgtct atgagaccga
     1801 accggtcggc ggtcccctgc gacaggggcc gtatctgaac atggtggccg tcctcgacac
     1861 cgacctgtcg ccgaaggacc tgttgggtct gtgtcatcgc ctcgaggcgg cggcgtatcg
     1921 ggtgcgcgag gaacactggg ggccgcggac cctcgatgtc gacatcatct gggtcgatgg
     1981 tgtggagatg gacgacccca acctcaccct tccccacccg cgttggaagg atcgtcggtt
     2041 cgtactggtt ccgatgcagg aggtcgcccc cgacctcgtc agcgatcgcg acatcgagac
     2101 cgcggacggt cgggtatggc tggcggaggg gttggcgtga ccacgaaggt tcgcatcgtg
     2161 ggtgcgggcc gcgccggcgg cgcgttcgcg ttggcgctgc ggcgcgccgg ctggaacgtc
     2221 gacgggccga tcgggcgcga ccacgccgcg atcgaggcgg cggcagacgg ggtcgatttg
     2281 gtgttggtgt gcgtgtccga cccggcgatc gccgaggtcg cctcgacgat ccggcccggc
     2341 gacgcggtgg tcgcccacgt ggccggcgcc gtcggactcg acgtgctcgc cccccatcgg
     2401 cgcgtcggcg ccatccatcc gctcgtgtcg ttgccctcgg ccgagttggg cgccgagcgg
     2461 ctcgtcggag cctggtatgg aaccggcggc gatccgatcg tgtgggaggt ggctcgagcg
     2521 ttacgaggtc gctcgtttcg catctcggat ctcgaccgtg tcacctacca cgccgcagct
     2581 tgtgtcgcag cgaatcatct cgtcgccctg ctgggtcagg tcgaacggct cgcagcgtcg
     2641 atcggcgccc cgatcgacgc ctatctcgac atggccgcgg ccgtcgtcga gaacgttcgc
     2701 accctgggag cggccgaggc gctgaccggc cccgcggcgc gtggcgacct cgcgacgatc
     2761 gagcggcatc tcgaggccat cggccccgac gagcgcgaca cctaccgtgt gctcgcacgc
     2821 gaggccaccc gcctggccgg ccgcgacctg ctcgcaccgc tcgaccggat agagggctga
     2881 gcggtggaga tcatcgaagg aatcgccgag cttcgtgagg ccctcgacga ccaccgcgcg
     2941 aacggtcgcc gggtcggatt cgctccgacg atggggtacc tccacgacgg ccacgagtcg
     3001 ctgttcaaac gcgccgttgg cgactgcgac gtcgcggtcg ccagcatctt cgtcaacccg
     3061 ctgcagttcg gcgtcggcga ggacctcgac gcgtatccgc gcgatccgca tggcgatcgc
     3121 gaacgggcgc aacgctgcgg aatcgcgtac ctgttcacgc cgtcggtgga ggagatgtat
     3181 ccccgaccga tcctcacgac cgtgtcggtg cgttcgctcg ccgcggtgat ggagggcgcg
     3241 agccggccca cccatttcga cggggtggcg acggtggtga cgaaactgct gtcgatcgtg
     3301 ggcccctgtc gggcgtattt cggcgagaag gattatcagc agcttgcgat catccgtcag
     3361 ctcgccttcg acctgtcgct ccccgtcgag atcgtcgggt gcccaacgat tcgagaggac
     3421 gacgggctcg cgcgatcgag ccgtaacgtg tatctgagcg acgctgaacg tgccgtggct
     3481 ccggtgctgc accgagcgtt gcgccacggg gccgacctga tcgtcggcgg gcagcgcgac
     3541 gccgaggtgg tgcgccgagc gatggcggag gtgatcgagg cggagccgct cggcgaactc
     3601 gattacgtcg aggtggccga cggtgcaaca ctcgaaccgc agcttcgctg tgatctttcc
     3661 agcagacttt tcggagcggt gcgctttggc cgcgcccggc tgatcgacaa cgtgggaatc
     3721 gagggcaggt gagcacacaa tcgagcgagg aactcgatct gttggtggtg ggaagcggcg
     3781 tggccgggtt gtcggccgcg gttcgggccg ccgaggtcga aggactcagc gtcggggtcg
     3841 tcaccaaagc ggaactcgac ctggcgacga cccgctgggc tcaaggtggc gtcgctgcgg
     3901 cgttgtcggg tgaacaggag gagaccgatc tccatctcgc cgacaccctc gccgccgggg
     3961 tcggactgtg cgatatcgaa gcggtgcggg tgctcgtcga cgagggcccc ggccgggtgc
     4021 acgatctcat cgcgctcggt gcgctgttcg atcgagacga gcacggcgag ttggaactgg
     4081 cacgagaggg cggccactcg gtgccgcggg tggtgcacgc aggcggcgcg gcgaccggtg
     4141 cggagatcga acgggcactc gtcgtggcgg tgcgcgaacg ggttcgcgag gtacacgagt
     4201 tcaccttcgc gctcgacctg atcgtggagg atggccgttg ctgcggtgtg acggcggcac
     4261 gcgacggtcg ggtgttcgag gtgcgggcac gcaacgtgtt gatcgccacc ggcggcgccg
     4321 gacaactctt cgcggtgacc acgaatccac cggtggccac cggcgacggt gtggctatgg
     4381 ccttgcgcgc cggggtggcc gtggccgatc tcgagttctt ccagttccac ccgaccgcac
     4441 tcgatcatcc ggcgatgccg cgagcgctgt tgagcgaggc gttgcgaggt cacggtgcgc
     4501 tgcttcgcga ttcgaggggc gagcgtttcg tcgacgagtt gttgcctcgc gacgtcgtga
     4561 gtcgtgcgat caccgccaag atgctcgagg agggcagcga tcgcgtgtgg ctggacgcga
     4621 cggtgttgaa ggacttcgac gtacggtttc cgacgatcct cgccgatctc gaaagcgcgg
     4681 ggctcgaccc ggcccacgat tacctcccgg ttgcgccggc agcccatcac cagtgtggcg
     4741 gcatcatcac cgatctcgac ggtgcgacgt cgttgcccgg cctgtgggct gcgggagaga
     4801 cgagttgttc gggggtgcac ggcgccaacc ggcttgcatc gaactcgctg ttggagggca
     4861 tggtgttcgg cgcccgcgtc accgaggcga tctcgcgggg tcaacgcggt ccgtgcgaca
     4921 ccggggcgat gcgttcggtg aaccgcgagg gctcgaccga tcgcgtgatc ggcggtcggc
     4981 gcatcacggt ggccgcgctc gagacgtcga cggcgaccga tctcgggcgg gacgcggtcg
     5041 acgaactgcg acggcaggtt cagcgcacga tgaccattca cgccggcgtg ttgcgtaccg
     5101 cggagtcgat cgacacggct cgcaacgatc tcgccgaggt gtgggcacaa ctgccggcgc
     5161 actccacgga tccggcggtg tgtgagctga tcaacctggc cgtggtagga gtcgcgttgt
     5221 tgggcgcggc gcaggctcgc gaggaaagcc ggggggcgca cacccgaacg gatttcccgg
     5281 ggacccacga ggaattccga gttcgtttgg tgattggatc gtgatgaaca cctactttga
     5341 ccctccgctg gtgctcgtgc gcgatgcggt tcggcgcgcc ctcgaggagg accttcatcc
     5401 cctcggcgac ttgaccgccg atctgctcga ccccgaagcc cgcacggtcg cccggttcaa
     5461 cgcccgcgaa gcgggtcggc tcgcaggaac ggtgtgtgcc caggaggcgt ttgcgcaggt
     5521 ggacgattcg atcgaggtcg aatgggtcaa gcgcgacggc gacacgctgg cccccggcga
     5581 cgacatcggg ttcgtgcgcg gccgcctcgc cccggtactg accgccgagc gcaccgcact
     5641 caacttcctc gggcacctgt cgggtatcgc cacgctgacg aatcgctatg tcgtggccgc
     5701 cgaggcgggt ggcggtgcga aggtgtggga tacgcgcaag accacgccgg ggctgcgggc
     5761 gatcgaaaag gcggcggtac gggccggtgg cgggcgcaat catcgcggca acctcagcga
     5821 ctggatgttg ctgaaggaca accacatcac gggcatcggc atcaccgagg cggtgacccg
     5881 ttgcctcgac ctgtggccgg cgcgcacggt gcacgtcgaa tgcgatcgtc tcgatcagat
     5941 ggtcgaggcc atctccgccg gggcccacgc cgtgttgttg gacaacatga gcccggatca
     6001 ggtgcgcgag tgcgtggcgg cggccagcga gttggcggat cggggcttcc gggcactgat
     6061 cgaggtgtcc ggtggggtca ccctcgacac gatcggcgag tactccggca ccggggtcga
     6121 ccagatctcc atcggtcgaa tcacgaactc cgctccggtg ctcgacatcg gcctcgacat
     6181 cggcccgccc gtagcctgat ctgtattcga agggttcggt agcgcagtcc tgtaccgacc
     6241 gtagttagcg acatcaacca ttcaggttcg acaaaaggag acgccggtgc ttctggcgat
     6301 cgacgttgac aacacccaga ccgttatcgg gctctacaag cgcggcgagg aaagccatga
     6361 gctgctcgac cattggcgca tcgccacgaa cgcgacgcgc acctgtgacg aactcgcact
     6421 gttgttcagc gagtttctgc tgagcgccgg ccacgattgg cgccaggacg tgtcgggggt
     6481 ggcggtgtcg tccggggtgc ccgatgtgac cttcgagctg cgccgcatgt gcgagcggta
     6541 cctcgacatc gatccggtgg tgttgggcgc cggggtgaag accgggatgc cggtgctgta
     6601 cgacaacccg aaggaggtcg gagcggatcg catcgcgaat gcggtcgggg cctacgacct
     6661 gtacggcggc ccgacgatcg tggtcgattt cggtaccgcg accaccttcg atgtgatctc
     6721 ggctgccgga gagtatctgg gcggtgcgat catccctgga atcgaaatca gcctcgatgc
     6781 gctgttcggc cgtgctgccg cgctgcgccg ggttgagctg accgagcccc gctcggtcat
     6841 cggcaagacg acgatggagt cgatccagtc gggcgtgctg tacggctggg cgtcgatgat
     6901 cgacggcatg agtgaccgaa tcgaagaggt cgtcggcgat gccaccgtca tcgccacggg
     6961 cggcctcgcc gatctggtcg cgccgttgtc cgacgccatc gaacatgtcg agccgtggct
     7021 cacgctgcac ggcctgcgcc tcatctggga gaagaatcga tgaccaccga gccgaaccac
     7081 accaccgccg actcacctcc gagcgccgaa acgccggcgc tgccgcatcg cgccgaggtc
     7141 accgatactg ccgctgccct cgccgatcgc ttcgcaggcc tggagccggg caccgagacc
     7201 ggtgtgcgag cagcgatcgc cggtcgactc atgttgcggc gcgtgcaggg caagttggcc
     7261 ttcggcaccc tcgcggatca gacgggtcgg attcagctgt tcgcgccgag ccagacgacc
     7321 cccgatttcg aacgcttcac cgccttgagc ctcggcgatt ggatctcggt gaccggcgag
     7381 gtgatgacga ccaaacgggg cgagttgtcg gtgcgcgtcg atgactggac gctgttggcg
     7441 ccgacccgtc ggtcgttccc cgacaagtgg cacggcatca ccgataccga catgcgctac
     7501 cggcagcgtt acgtcgacct gtgggtgacc gacgaggcac gggccgcatt caccaaccgc
     7561 agtcgcatcc tgtcgctgac ccgacgcttc atggaggacc gcggctacct ggaggtcgag
     7621 acgccgatcc tgcacccgct tgccggcggg gcgttagccc gtccgtttgc gacgcatcac
     7681 aacgcgctcg attccgagct gttcctgcgc atcgccccgg agctctacct gaagcgcctg
     7741 gtcgtcggcg ggttcaccaa ggtgttcgag atcgggcggg tgtttcgcaa cgaggggatg
     7801 tcgacccgac acaaccccga gttcaccatg ctcgagtgct acgaggccta cggcgactac
     7861 cacgtgcaca tggatctcac cgagcaactc atcgcgcacc tggcgacgca gatctgcggc
     7921 tccaccgtgg tcacctacgg cgatcgcgaa ctcgacctgt cggccccgtg gcggcgcgct
     7981 ccgttggcga cgctgctgag tgagcagatc ggggtcgagg tcgatgtaca cacgccggtc
     8041 ggggagcttc ggcgtctgtg cgacgaacac gagacgccgt ggaaggagtc ctacggcgca
     8101 gggaagttgc tgctggagct gtacgaaaag accgtcgagc acacgctctg ggagccgacc
     8161 ttcgtgatcg actaccccaa ggaggtctcg ccgctgtccc gggtgcaccg cgacgacgag
     8221 ttgttgaccg agcgtttcga ggggatcgtc gccggacggg aattgtgcaa cgggttctcg
     8281 gagttgtgcg accccgatga tcagcgggag cgcttcgagg agcaggcggc gcagaaggcc
     8341 gatggcgacg acgaggcgat ggtggtcgac gaggactatc tgcgcgcgct cgactacggg
     8401 ctgcccccaa cggtggggct cggcatcggg atggaccgtc tcgtgatgtt gctcaccaac
     8461 acgaccacga ttcgcgacgt ggtgttgttc ccgacgttgc ggccggaatc cccggatgtt
     8521 cccgtggccg atgtgggccc atccagcgac ggcccatcca acgacggcgc ggccgacgcc
     8581 gggtcctgac cgtgggcgcc gccgcggcgg tttcggtccg gggggtgtcg aaatcctttg
     8641 ggtcgaacat ggtgctcgac gagtgcacct tcgacgtcgc gcccgccgag atcgtggccc
     8701 tgctcggccc ctccggatgt ggaaaatcga cgctactgcg catcattgcg ggtttggagc
     8761 gactcgacgg gggcgtcgtg gagttggcct cggcacacgg tgcgcagcgc gtcgttgcgg
     8821 ggtcagaccc cgcgtcgggt aaagccgtgc acgtcgcgcc cgaacggcgc ggggtcgggc
     8881 tggtgtttca gaacggggca ctgttcgcgc acctcgacgt cgcccacaac atcgccttcg
     8941 gtctggcgaa gggtgaggac cggacgtcgc gggtgagcga actgctggcg atggtcgacc
     9001 tggcgggatt cgaagaccgg tatccggatc aactgtcggg gggtcaacaa caacgcgttg
     9061 cgttggctcg ggcgttggcg ccgaggccgg cggtggtgtt gttggacgag ccgttctcga
     9121 acctggacgc ccggctgcga cggtcgttgc ggcgcgaggt caccgaggtg ctgcgacgct
     9181 cggcgactcc gacggtcatc gtgacccacg atcgggacga ggcgttcgcg gtggcggatc
     9241 gcgtcgcggt gatgcgcgat ggtcgcatcg cccagtttgg cacgcccagt gacgtctacg
     9301 accgcccggt cgatgactgg gtcgcccggt tcgtgggcga cgccaacttc ctcgaagtgc
     9361 gtaccgtggg cgatcgcgag gtcgacacgt tggccgacac cgtggtcggc ccggttcgtg
     9421 tcggcgctcc ggtcgcggcg ggggacacgg tcatgatccg acccgagtgc ctcacgttcg
     9481 agcgcacgag cgcgtggccg ctcggtcggg tcgtcggggt cgaatacacc ggagcgacgc
     9541 tgctcgtgga tgtggatgtg gacgtgggtg ccgaggttga tgccaaggct gatgcaggtg
     9601 ccgtcgtcac tcacggctcc gtgcggctgc gttccgagca gtcgacggcg ggcgcggccc
     9661 cgctacgcat cgacgatcgc gttgtcgtgc acgtccatcc cgccaccgtt gcccatcggg
     9721 tcgaagtgtc gtgagcatcc ggtggatgac tcatgaactc gacctcatgt actaacacat
     9781 ttatccaaac tgttagtaga ataggacaat gatcacagat ccagcgctgc cgattgagcc
     9841 cgtcaggctc gaggatcacg agtggatcgc ggagtccgac gggatgtcga gtcgcgcgcg
     9901 ggtcgcggcc ggttccggcc cctaccaatc ggtggtgccg gcgacgatcg ccagctaccg
     9961 cccgacggtt ccgtccgacc tcaccgcgga catcgaggaa gcgtcggcgg ccttggcgcg
    10021 attcgactcc tacacacagg ccgtcctcgg tatcgagcac cctgcgctcg gcccgatgtc
    10081 ttcgatcctg ctgcgcaccg aatcggcatc gtcgtcgcag atcgagaacc tcaccgtggg
    10141 ggcgcggcag ctcgcactcg ctgagattgc tcagccgagc agcaccaacg cgaagatcgt
    10201 tgctgcgaat gtgcgggcga tggaagccgc cctcgtactc gccgacaact tggatgaagc
    10261 ggccgtcttg gcgatgcacg ccgaactcat ctcccgccaa ccgggttggg agcaacacgc
    10321 tgggtgctat cgaaacagcc ttgtctgggt cgggaccagc gggatcagcc cacggggggc
    10381 aacccatgtc gcgccacaac ccgaacttgt ctcgtcggcc atggacgacc tccagcagtt
    10441 cttggcacga gacgacctgc ccgtgctcct ccaagcggcc gtggcgcacg cccaatttga
    10501 aacgatccat ccctttgcag acggcaacgg tcgtaccggc agagcgctgg ttcatgcgat
    10561 tcttcgcggc aagggactca tgacccacac gacagcgccg gtgtcggccg gcctcctgac
    10621 cgacaccgag tcgtacttcg gcgccttgtc gacgttcaga gcgggcgatg ctcgaccgat
    10681 catcgaggag ttcgctcgtg cgagccgcta cgccgcgacc accggcaccc gtctggttga
    10741 cgatctcgcc gcgcaggtcg agcgggcacg ggtgcagatg gcgggcctgc gtccgcaagc
    10801 gtcggcatgg cgggttctgc cccatctcgt cgcgcaaccg gtgatcaacg cccggtatct
    10861 cgtcgaccat ctgggaatga gcgacatgac cgcccaacgc gcactgggtc agctcaccgc
    10921 tgccggagta cttgtcgaac gcacggggca ccgacgaaac cgcatttggc agcactcggg
    10981 catcttgggt gttctcgacg gatacgcgca gcagctgcga cgaagctgag cgacgcaatc
    11041 cgaatggtgg ttcaggagtc gccggggtgt tgtgctgacg agatggtgta gaaccgggct
    11101 tcgacgctcc agccgacgct ccggccgatg ctccagccga cgcttcagtc gacgattcga
    11161 tcgatgagtc cgaggtggag cgcctcggtg gcggtgagcc tcgtttgggt gagggcgagc
    11221 cacatggttc gttgtcggcc gatccggcgg gggatcgaca cggtgccccc ggcgccggga
    11281 atcaacccga gcgacagttc cggaagttga atgacggtgt cggcggcggc ctcgacctgc
    11341 cgagcgaagg ccgcgatctc gatgccggct ccgacgcaat gcccgtgcag gtgaacggtg
    11401 aggatgtcgg caacctgatg tgcctcccaa ccggggtggc gcgtgagtcg ggccgccatg
    11461 gccgtggccc cgctcggggc ggtcccgaac tcggcgaggt cgcctcccgc gcaaaacgac
    11521 ggtccgttgc cggtgagggt gattcgcgcg accgacgggt cgatgcgagc gttgcggaag
    11581 gcgtcgacga ggagatcgcg catcgacgaa tcgatggcgt tgcgcgcctc cgctcgattc
    11641 aacttgagct gaaggatcgg gccgtgtttg cgaatcacga ccccacggcc gacatgatcc
    11701 ttgacggtcg ccggcccccg acgttgcagc caaccgcgga agtcctgtgc cccgagcagc
    11761 atcgaatagc tcgtcgcctc ggcggcgagc ccttcccaca ccggtcgaac gaacgacgag
    11821 cgcagcagtg ttgccaggct gaacgccgcc agcgggtgcg ccgccacggg ttcggtgatg
    11881 gatttcaact cgtcgtggat ccacggcgac ggcgggtcgg gcgcggtggt gaggctgatg
    11941 tcggtcgcct tcgcgagccg atcgaatcgg cgtcggtcgg cgagtcgccc ggtctcgtcg
    12001 accaacacgg ttaccgtcgg ggcgaccgat gcaaccgccg cgatcgccgc caggtcccgc
    12061 tccgactcgt cgccggtggg aacgagcacg aggggctcgg acgcgtgaag gatcgacgcg
    12121 tcgtcgagca ggctcgatga ttcgagcgcg atgaggcgcc gtaggtctcg cacttccacg
    12181 gcgacgacgc tacctgttgg ccgtacaatc gctgcgttgt gggcgattcg atggttggtc
    12241 tgacaccgga ggccgaatgg gcgcgcagtg gtgtcgtgac gctcacgggc gatgtcgagg
    12301 ggccagcgct gcttcctccc ggtgccgctg ctgcgatggt cgaggccgag gcggcggcct
    12361 tcggcctcga cgcccgagtg ctgtcggagc gagccgcgct catggggttg caccgcaacg
    12421 ggcgtacttc gtgtggcggg gcatcgtgcc tgctggaggt gtgcgatggt tgggttgtgc
    12481 tgtcgctggc tcggggcagc gacatcgagc tcgtgccggc gctcattggt ggggagaagg
    12541 ttccgagcgg gtcgccgtgg gacgccgtcg tcgagttcgc tcggaccgtg tcggctgcgg
    12601 agtttgtcga gcgggcggcg gtgctggacc tgcccggcgc ggtcgtcgac gcatcgcccg
    12661 cgccaagcga tgtcgagccc ggggagttca ccgtcagtcg ccgtgactcc gcgaggtccc
    12721 gtcccgtggc cggagcgggg gcggcgcccg atggcccact cgtcgtcgac ctgtcggcgt
    12781 tgtgggcggg gccgctgtgc agtcacctgt tgcaacgccg gggggcgcgg gtgattcggg
    12841 tcgagtcggt ctcgcgcccc gacggtgccc gacgcggaaa ccggtcattt ttcgacctgc
    12901 tcaatgcggg caaacagagc gtggcgttcg atttcgatag ccctgaggac cgtcgccgcc
    12961 tgcgcgggtt gatcgatgcc gccgacgtcg tgatctcctc ggctcgtcgt cgcgccatcg
    13021 agggacttgg gctcgacgtc ggcgcattcc tcgcgggggg gaccgatcgc gtgtgggtgg
    13081 cgatcaccgg gttcggatgg aactcgaacc gggtcgcctt cggcgatgac gccgcggccg
    13141 cggcagggct ggtggcgtgg ggggac
//
LOCUS       DAOOMA010000010        12619 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_5515_length_12619_cov_4.720073, whole genome shotgun sequence.
ACCESSION   DAOOMA010000010 DAOOMA010000000
VERSION     DAOOMA010000010.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 12619)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12619)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12619
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5515_length_12619_cov_4.720073"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..510)
                     /locus_tag="PLP95_00635"
     CDS             complement(<1..510)
                     /locus_tag="PLP95_00635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HPB44341.1"
                     /translation="MRSLPSAVELTEGILRGDRTVLARGITMIESRRGDHRAIAAEVL
                     DNIMAATGSAVRVGITGVPGVGKSTFIDQFGTNLTAAGHRVAVLAVDPTSQRTGGSIL
                     GDKTRMDRLAVDARAFIRPSPAGSTLGGVTRSTRETMLLCEAAGFDVILVETVGVGQS
                     ETIVADMVDF"
     gene            597..1277
                     /locus_tag="PLP95_00640"
     CDS             597..1277
                     /locus_tag="PLP95_00640"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HPB44342.1"
                     /translation="MARLLLVEDDDAIAKPLARALAREDFEVTRVSNGTDAVATFRGG
                     NVDLVLLDLTLPDIDGLDVCRRLREIDNTTPIVMLTARSDELDIIVGFDAGADDYLGK
                     PFSMAELTARLRARLRLNQHDDSILVADGLSIDTRARRSTLDGDELHLTPKEYEILVM
                     LMTSRDQVVSRQRILLDLWTGDLEAASRSLDVHMSSLRKKLGEATDTPRFIHTVRGVG
                     FRFEATGA"
     gene            1284..2522
                     /locus_tag="PLP95_00645"
     CDS             1284..2522
                     /locus_tag="PLP95_00645"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HPB44343.1"
                     /translation="MRRRLVTSFLGVTAIVLVLVAIPMGYLLQRVATDEVRSQLADQA
                     SFVLNRAVAADHDGVRLSVSDLEALVLGDTGAEIRYADGAVVQSNPEGDSEVISATIR
                     NEGNVSTVRTFTGVDAIQQRMREPLLVLGGLGLAGLMATWLLAQAEARRLDGPLRDLA
                     QTAERLGSGDFSVTAPRSGIAELDSLATTLDRTASRIDELVRAERSFTSDAGHQLRSA
                     LTGLRLRIEELTMTDDPDTREEAIAALEQADRLSSTIDDLLRLSRTGRAGVTTRFELR
                     PFITAHINDVAITLRDQRRTIEFAPGPALSIAAAPGAVGQAIDVLLWNAVRHGRGLIV
                     VRAEMDSRWAIITVTDEGSVDPETVGGFFTEAERHSAHGIGLPLARRVVEAEGGRLDV
                     IVTSPTTFRIRLPLATLPDD"
     gene            complement(2597..4774)
                     /gene="scpA"
                     /locus_tag="PLP95_00650"
     CDS             complement(2597..4774)
                     /gene="scpA"
                     /locus_tag="PLP95_00650"
                     /EC_number="5.4.99.2"
                     /inference="COORDINATES: protein motif:HMM:NF006944.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004494 - methylmalonyl-CoA mutase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase"
                     /protein_id="HPB44344.1"
                     /translation="MTGLPDYTAVAGPIVSDDAVDGAPSRSAAAGEVWETPEGIAVAP
                     LYTAADTAGLDFLDTYPGMAPFLRGPYSTMYVNQPWTIRQYAGFSTAEDSNAFYRRNL
                     AAGQKGLSVAFDLATHRGYDSDHPRVGGDVGMAGVAIDSIYDMRVLFDGIPLDQMSVS
                     MTMNGAVLPVLALYIVAAEEQGVAPDKLAGTIQNDILKEFMVRNTYIYPPAPSMRLIG
                     DIFAYTSQRMPKFNSISISGYHMQEAGATADLELGYTLADGVEYIRTGQVAGLEVDAF
                     APRLSFFWAIGMNFFMEVAKMRAGRLLWAKLVKQFDPQSNKSLSLRTHSQTSGWSLTA
                     QDVYNNVIRTCVEAMASTQGHTQSLHTNALDEALALPTDFSARIARNTQLFLQQESNT
                     TRVIDPWGGSYYVERLTYELARKAWAHIEEVEQLGGMAKAIEAGIPKLRIEEAAARTQ
                     ARIDSGRQSVIGVNKFRPDGDEMIEVLKVDNATVRANQIAKLERLRAERSTDAVQSAL
                     EALTKCADGGAGNLLDLAVDAARKMATVGEISDALEKVWGRHRAEIKSISGVYRSEMG
                     ADNETVERVRRKVEAFEAADGRRPRILLAKMGQDGHDRGQKVVASGFADLGFVVDIGP
                     LFTTPEEAAQQAVDADVHIVGPSSLAAGHLTLVPELVAALEKLGRSDIMIVVGGVIPP
                     QDFDALYEAGATAIFPPGTVIAAAADDLLDKLAAQYGYDLGVA"
     gene            complement(4771..6699)
                     /locus_tag="PLP95_00655"
     CDS             complement(4771..6699)
                     /locus_tag="PLP95_00655"
                     /inference="COORDINATES: protein motif:HMM:NF013781.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HPB44345.1"
                     /translation="MTTSELTLAAEFPSGSREQWLALVDTVLKGADFDRKLVSNTYDG
                     IAIQPLYTRQDRPPAEQLPGQGDLTRGVGGPRGDLPWSIATIQRHPLVEEANRQVLTD
                     LEGGANSLVLRLDVHGAGAVDGTVIGAADDLAAVLEGVYLDLISVTLEAGPLGATAAE
                     LLVALYDVKGVSPAERAGSLGLDPIGALAATGALPQGLDGAFAELRELANRVSDAPNL
                     RTINVDTAAVVDAGASEVEELAVAIATALEYVRALCSDRPLEAAFGEIAFTLQSDADF
                     FAGVAKLRAARRLWAHAVRAAGVDPVALATPFASRTATRMMSQRDPWVNMLRTTAACF
                     AAGVGGASSVAVQPFDSAVGLSDDLARRIARNTQLILQEEANLGAVIDPAGGSNYIEA
                     LTTELVERSWARFQEIEAAGGMVAVLTSGDEAETLQLTREARLLNIARRRDPLTGVSE
                     FPNIDEAPLTREQIDLVALRNAAVAHGVVPAGPATTIEALPAVRNGSEFEALRDASDA
                     LAATDERPSIFMANLGPVAAHTARATYAKNFFEAGGVKAVGDVGHDTADAAAEAFIAS
                     GLEVAVICGSDSLYAELAAPVATALKKAGARRVYLAGNPGDARGDYEAAGVDEFVYVG
                     VNVLDTLTRLLKMQGALS"
     gene            6883..7389
                     /locus_tag="PLP95_00660"
     CDS             6883..7389
                     /locus_tag="PLP95_00660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HPB44346.1"
                     /translation="MTELSVGLLILRVAFGLTMAAHGYGKFFQGGRIPGTAGWFDSMG
                     MRPGKVHAVLAATSEVASGLAIALGLLTPFAGGLFIALMIVAGYTVHAENGFFIVKSG
                     WEYNFILAVFGLGIAMVGPGEYSLDHAFGIADTLSGWTGLIIALIVGVGGGIGQLVVF
                     YRPPAKTS"
     gene            7475..7984
                     /locus_tag="PLP95_00665"
     CDS             7475..7984
                     /locus_tag="PLP95_00665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HPB44347.1"
                     /translation="MGNTDHVPSDRRRCDGGIHDTDNLRTTLARIAVRLEAQRCELFE
                     VDEHRATLLDRWVASGMAIALAERRDVPLHWFPWSLGSVRPREYLFVRNAGALPMALV
                     DHTERPEHRVVSGADLGVGAALMVPLTLTGHPIGALCVYWSLERQSWESESRDTVASW
                     ALDALLAPR"
     gene            complement(7912..9231)
                     /locus_tag="PLP95_00670"
     CDS             complement(7912..9231)
                     /locus_tag="PLP95_00670"
                     /inference="COORDINATES: protein motif:HMM:NF013740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HPB44348.1"
                     /translation="MGDDPSDLSISSPAPSASDVASDAASVMMNDGLPDTWNAARPPR
                     WFPRAVLYVMLGAIGFMVSRTVFANLSDLLVLLLVSLFLSFAVEPAVDRLSARGMKRG
                     LATGLVFLLLLVGSFVLVWVIVDLVVQQVTSLVNDAPRLIQDATRWFNDTFHANVTTD
                     DLVKQVREYEGDLTRYAGDVGGRVMSVTGTALGVVFNMFTVFLFTFYLVAEGPQFRRT
                     ICSVVPQDRQVMVLGLWELAISKTGGYLYSRTILAVVSSILSWIAFAIIGVPSPLALA
                     VWMGIVSQFVPVVGTYIGGALPVVIALLDSPPRALAVFVFMIVYQQFENYVLAPRVTA
                     RTMEIHPAVAFGAVLAGGSIIGPIGALLALPAAAIIQAFVSSFLNRHELVESELTQRM
                     VSSSGSDDAIPPTEHRGGAFWERLQRGANKASSAHDATVSRDSDSQD"
     gene            complement(9224..9943)
                     /locus_tag="PLP95_00675"
     CDS             complement(9224..9943)
                     /locus_tag="PLP95_00675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HPB44349.1"
                     /translation="MSDDRLYFRQLLAGRDFAQTDVIAGQMANFSYLIGDRETGECLV
                     VDPAYDVDGLIASAAVDDMTIAGALVTHYHADHCGGSMMGFNLEGIEALLNRVQVPIH
                     VQETEVEWISKTTGVTAPDLVGHRTGAHVMIGDVRVDLIHTPGHTPGSQCFLVHDHLV
                     SGDTLFLEGCGRTDLPGGNAEDLYYSLTQRLAKVPDSAVLYPGHRYSAMPSAPMGETR
                     AQNFVFRPQSVEQWMMMFGNG"
     gene            complement(9940..11190)
                     /locus_tag="PLP95_00680"
     CDS             complement(9940..11190)
                     /locus_tag="PLP95_00680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015798139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HPB44350.1"
                     /translation="MSHLSPVWTNLTDLDVVSGEGCWITSASGERYLDFTAGIAVTST
                     GHCHPTVVEAIRTQAGRFIHAQVNCYRHDLLEPLAARLADLTPAGIDTFFFSNSGAEA
                     VEAAVKLAKHATGKSNIIVMDASFHGRTHLAMAMTTSKTAYRRNYAPLPAGIHVTPFP
                     RSVGVGTLDERIDAALAQLRRLLTTQTAPQETAAIILEPVLGEGGYEPAPVRYVQGVR
                     AICDEHDLLLIADEVQTGFGRTGTMFAMDQTGVTPDIIVMAKGFASGFPISAIGSPRA
                     LMDRWITGTHGGTYGGNPLGCAAALATIEVVTAEGFLDNVNARGEQLRAGLRGLAAKH
                     PNLVDVRGPGLMVAAEFRTAEGEPDAATVTSVISHAFSEHRVLLMNAGTDGNVIRFMS
                     PLIVSADEIDLALAAVGAGLASAS"
     gene            11300..12259
                     /gene="pheA"
                     /locus_tag="PLP95_00685"
     CDS             11300..12259
                     /gene="pheA"
                     /locus_tag="PLP95_00685"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: protein motif:HMM:NF008865.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HPB44351.1"
                     /translation="MSSHQPRTGTVSPSASVGFLGPIGTFTEQALLGQPDLAAATRIE
                     FDNFSELLFSVENGELDYGFVALENAIEGQVNITADTLTFDVDLLIQREVVLPIEMTL
                     QVKPGAVLDKITRVSSFPHAYAQCREWLRANLPNVELVPATSTAGASEIVATGNDATI
                     AAIANARAAELHGLDIIARDIADHPENATRFALVARRGIPAPSANDKTSIVVFQRADV
                     PGSLLSILQEFAARQINLSLLLSRPTKKSLGDYCFLIDIVGHLADPLVADALRSLKMK
                     HDVKFLGSYPAGVALDDAEREAAEAHLDEAKAWIRSLREQLDE"
     gene            12320..12407
                     /locus_tag="PLP95_00690"
     tRNA            12320..12407
                     /locus_tag="PLP95_00690"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:12354..12356,aa:Ser,seq:tga)
     gene            12462..12552
                     /locus_tag="PLP95_00695"
     tRNA            12462..12552
                     /locus_tag="PLP95_00695"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:12498..12500,aa:Ser,seq:gct)
BASE COUNT         2234 a         4200 c         3973 g         2212 t
ORIGIN      
        1 gaagtcgacc atgtcggcga cgatcgtctc ggactggccc accccgaccg tctccacaag
       61 gatcacatcg aatccggccg cctcacacaa caacatggtc tctcgcgtcg agcgggtcac
      121 gccgccgagg gtcgatccag cgggagacgg acgaatgaac gcgcgggcat ccacggccag
      181 ccgatccatc cgtgtcttgt cgccgaggat cgaaccaccg gtgcgctgcg aggtcgggtc
      241 gaccgcgagc acggccaccc gatggcccgc ggccgtcagg ttggtgccga actggtcgat
      301 gaacgtcgac ttgccgacac ccggcacacc cgtgattccg acgcgcaccg cggagcctgt
      361 ggccgccatg atgttgtcga gcacctccgc ggcgatggca cggtgatcgc cgcggcggct
      421 ctcgatcatg gtgatcccgc gggccaacac ggttcggtcc ccccgcagaa ttccctcggt
      481 caactcgacg gcggacggca acgaacgcat agcgagatgg taggcaaccg cgcatggttc
      541 aaggaatccg ctccccaacg gtgcacgacc gtcgacttcg gtttaccctc gatgaaatgg
      601 cacgcctttt gttggtcgaa gacgacgacg cgatcgcgaa gcctctcgct cgagcactcg
      661 cacgcgagga cttcgaggtc acccgagtct caaacggcac cgacgcggtc gcaaccttcc
      721 gcgggggaaa cgtcgatctg gtgctgttgg atctcacgct gcccgacatc gacgggctcg
      781 acgtgtgccg caggttgcgt gaaatcgaca acacgacgcc gatcgtgatg ctgaccgccc
      841 gcagcgacga attggacatc atcgtcggat tcgatgccgg tgccgacgac tacctgggca
      901 aaccgttctc catggccgag ctgaccgccc gccttcgggc ccgtctgcgc ctgaaccaac
      961 acgacgactc aatcctggtg gccgatggtc tgtcgatcga cacccgggcc cggcgcagca
     1021 cgctcgacgg tgacgaactc cacctcactc cgaaggagta cgagatactc gtcatgttga
     1081 tgacgagtcg cgaccaggtg gtctctcgtc agcggattct gctcgacctg tggaccggcg
     1141 acctcgaggc agccagccgg agcctggatg ttcacatgtc gtcgcttcgc aagaagctcg
     1201 gggaggccac cgacaccccg aggtttatcc acaccgtccg cggtgtgggg ttccgcttcg
     1261 aggccaccgg cgcgtaagca tccatgcgtc ggcggctcgt cacctcgttc ctcggcgtca
     1321 ccgctatcgt gctcgtcctt gtggcgatcc ccatgggcta cctgctccag cgcgtcgcca
     1381 ccgacgaggt tcggtcacag ctcgccgatc aggcatcctt tgtcctgaat cgcgcggtcg
     1441 ccgccgatca cgacggtgtg cgactgagcg tttcggacct tgaggcgctc gtccttggcg
     1501 acaccggagc cgagattcgc tacgcggatg gggcggtcgt tcagagcaac cccgagggcg
     1561 attccgaggt gatctccgca acgattcgaa acgagggcaa cgtttcgacc gttcggacct
     1621 tcaccggtgt cgatgccatc caacagcgca tgcgtgagcc cttgctcgtg ctcgggggtc
     1681 tcggtctcgc cggcctcatg gcgacgtggc tgctcgccca ggccgaagcg cgacgcctcg
     1741 acgggccgtt gcgcgacctg gcccagaccg cggaacgcct cggttccggc gacttctcgg
     1801 tgaccgcccc tcgctcgggt atcgccgaac tcgacagcct cgccacgacc ctcgaccgga
     1861 ccgcctcaag aatcgacgag ctggtccggg ccgaacgcag cttcaccagc gatgccgggc
     1921 accagttgcg ctcggcgctc accggccttc ggttgcgcat cgaagaactc acgatgaccg
     1981 acgatccgga cactcgcgag gaagcgatcg ccgcactcga gcaggcggac cggctctcat
     2041 ccaccatcga cgacctcctg cggctctctc gaaccggacg ggcgggggtg acgacgcgct
     2101 tcgaactgcg gccgttcatc accgctcaca tcaacgacgt cgcaatcacc ttgcgcgacc
     2161 aacgccgtac gatcgaattc gccccgggtc cggccttatc gatcgcggcc gcgccgggcg
     2221 ccgtcggcca ggcgatcgac gtgttgttgt ggaacgcggt gcgccacggc cgcgggctca
     2281 tcgtcgttcg agccgagatg gattcccgct gggccatcat caccgtcacc gacgaagggt
     2341 cggtcgaccc cgagaccgtc ggcgggttct tcaccgaggc cgaacgtcat tccgcacacg
     2401 ggatcggact cccgctcgcg cgtcgagtcg tcgaagccga aggaggacgg ctcgacgtca
     2461 tcgtgacgtc gccgaccaca tttcggatcc gcctgccgct cgcgacactg cccgacgact
     2521 gaactcgacc gttcatcgga gagctcggcg agcatcggcg caaccgaggc ccgatcgccc
     2581 gatcggcgcc cgccggtcag gcgacgccga ggtcgtagcc gtactgtgcg gcgagcttgt
     2641 cgagcaggtc gtcggccgca gcggcgatca cggtcccggg cgggaagatc gccgtcgctc
     2701 cggcttcgta caacgcgtcg aagtcctgcg gtggaatcac cccgccgacg acgatcatga
     2761 tgtcggaccg ccccaacttc tcgagtgccg ccaccaactc cggcaccagg gtcaggtgac
     2821 cggcggccag cgatgatggg ccgacgatgt gcacatcggc gtcgaccgcc tgctgcgccg
     2881 cctcctcagg cgtggtgaac agcggaccga tgtcgacgac aaagccgagg tcggcaaagc
     2941 cggaagccac gaccttctgg ccacgatcgt ggccgtcctg acccatcttg gcgagcagga
     3001 tgcgcggtcg ccggccgtcg gccgcctcga aggcctcgac cttgcgccga acccgttcga
     3061 ccgtttcgtt gtctgcgccc atctcgctcc gatacacacc agagatcgac ttgatctcgg
     3121 cccgatgtct gccccacacc ttttcgagcg cgtcgctgat ctcgcccaca gtcgccatct
     3181 tgcgagcggc atcgaccgca agatccaaca ggttgcccgc accaccgtcg gcgcatttcg
     3241 tcaacgcctc aagtgcggac tgcaccgcgt cggtgctgcg ttcggcgcgc agccgttcga
     3301 gtttcgcgat ctgattcgcg cgtaccgtcg cgttgtcgac cttgagaacc tcgatcatct
     3361 cgtcgccgtc cggacggaac ttgttcacac cgatcaccga ctggcgtccg gagtcgattc
     3421 gggcctgcgt gcgagccgcc gcctcctcga tgcgcaactt cggaatgccg gcctcgatgg
     3481 ctttggccat gccgccgagc tgttcgacct cttcgatgtg cgcccaggct ttacgcgcca
     3541 gctcataggt caggcgttcg acatagtacg agccgcccca cggatcgatc acgcgagtgg
     3601 tgttggattc ctgctgcaga aacagctgag tgttacgggc gattcgcgcc gaaaagtcgg
     3661 tcggcaatgc caacgcctcg tcgagcgcgt tcgtgtgcag cgattgggtg tggccctggg
     3721 tcgacgccat cgcctcgacg caggtgcgaa tcacgttgtt gtagacgtcc tgagcggtga
     3781 gcgaccaccc tgaggtctgg ctgtgggtgc gtagcgacaa cgacttgttg ctctgcgggt
     3841 cgaactgctt gacgagcttg gcccacagca ggcgacccgc tcgcatcttc gcgacctcca
     3901 tgaagaagtt catgccgatc gcccagaaga agctcaggcg cggtgcgaat gcgtcgacct
     3961 caaggcctgc gacctgcccg gtccgtatgt actccacccc gtcggcaagc gtgtatccga
     4021 gttcgagatc ggcggtcgca ccggcttcct gcatgtgata gccggagatc gagatggagt
     4081 tgaacttcgg catccgctgg ctggtgtagg cgaagatgtc gccgatgagc cgcatcgatg
     4141 gtgccggcgg atagatgtag gtgttgcgca ccatgaactc tttgaggatg tcgttttgga
     4201 tggtgcccgc aagcttgtcc ggggccaccc cctgctcctc agccgccacg atgtagagcg
     4261 ccagcaccgg caacaccgca ccgttcatcg tcatcgacac cgacatctga tcgagcggaa
     4321 tcccgtcgaa gagcacccgc atgtcgtaga tcgaatcgat cgcgacgccg gccataccga
     4381 cgtcgccgcc gacgcgtggg tgatccgagt cgtacccccg gtgcgtcgca aggtcgaacg
     4441 ctaccgacag gcccttttgg cccgccgcca ggttgcgacg gtagaacgcg ttggagtcct
     4501 cggctgtgga aaagccggcg tactggcgaa tcgtccacgg ctggttgaca tacatcgtcg
     4561 aatacggccc acgcagaaac ggcgccatac ccgggtaggt gtcgaggaag tcgaggcccg
     4621 cggtgtccgc agcggtgtac agcggcgcga ccgcaatgcc ctcaggcgtt tcccagacct
     4681 cgccggccgc ggcggatcgc gatggagcgc catcgaccgc atcatcggaa acgatgggac
     4741 cggcgacggc ggtgtaatcc ggaagaccgg tcatgacaaa gctccctgca tcttcagaag
     4801 tcgggtgagg gtgtcgagca cattgacgcc cacgtaaacg aactcatcca ctccggcggc
     4861 ctcatagtca cctcgagcat cgccgggatt gccggccaga tagacgcgcc gtgcgccggc
     4921 cttcttcagt gccgttgcca ccggcgcggc caactcggcg tacagcgagt cggagccaca
     4981 gatgacggcg acctcgagcc ccgacgcgat gaacgcctcg gcggcggcgt cggccgtgtc
     5041 gtgaccgaca tcgccaaccg ctttgactcc gcccgcttcg aagaagttct tggcgtaggt
     5101 cgctcgcgcc gtatgagcgg cgactgggcc gaggttcgcc atgaagatcg acggacgctc
     5161 atcggttgct gccaacgcgt ccgacgcgtc gcgcaacgcc tcgaattcgc tgccgttgcg
     5221 caccgcaggg agcgcctcga tggtggtcgc cggtccggcg ggcacgacac catgggccac
     5281 cgctgcgttc cgcaacgcga cgagatcgat ctgctcgcga gtgagcggtg cctcgtcgat
     5341 gttcggaaac tcgctcaccc ccgtcaacgg gtcgcggcgc cgagcgatgt tgagcaggcg
     5401 cgcctcacgg gtgagttgca gcgtctccgc ctcgtccccg ctcgtcagca ccgccaccat
     5461 gcctccggcc gcttcgattt cctggaaacg ggcccatgac cgctcgacca gttccgtggt
     5521 gagcgcctcg atgtagttcg atccaccagc gggatcgatc accgctccga ggttcgcctc
     5581 ctcctgcagg atcagttgtg tgtttcgagc aatgcgtcgc gccagatcgt ccgacagtcc
     5641 aaccgccgag tcgaagggct gcaccgccac cgatgacgca ccaccgaccc ccgccgcgaa
     5701 acacgccgct gtcgtgcgca acatgttcac ccacggatcg cgctggctca tcatgcgagt
     5761 cgccgtccgg ctcgcaaacg gtgttgccag cgcgaccggg tcgacacccg ctgctcgaac
     5821 cgcatgggcc cacagccgac gagccgcccg caacttggca actccagcga agaaatcggc
     5881 gtcggactgc aacgtgaacg cgatttcacc gaaggccgcc tcgagaggtc ggtcgctgca
     5941 gagcgcccgg acgtactcga gcgccgtcgc gatcgccacc gcaagttcct cgacctcgct
     6001 ggccccggcg tcgaccaccg ctgcggtatc gacgttgatc gtgcggagat tcggcgcatc
     6061 agacacccga ttcgccaact cgcggagttc agcgaaggcc ccatcgaggc cctgtggcaa
     6121 cgcgcctgtc gcagcgagcg ccccgatggg gtcgagcccg agcgaccctg cgcgttccgc
     6181 ggggctgacc cccttgacgt catagagcgc caccaacaac tcagcggcgg ttgcgccaag
     6241 gggtcccgcc tcgagcgtga ccgagatcag atcgagatag acgccctcga gcaccgcggc
     6301 gaggtcgtcg gccgcaccga tgaccgttcc gtcgacggcc ccggcgccgt gaacgtcgag
     6361 tcgcagcacc agcgagttcg ctccgccttc aaggtcggtg agaacctggc gattcgcctc
     6421 ctcgacgagc ggatgtcgct ggatcgtggc gatcgaccat ggcagatctc cacgcggtcc
     6481 gccgaccccc cgggtcaggt cgccctggcc gggaagctgc tcggccggag ggcggtcctg
     6541 tcgggtgtac agcggctgga tcgcaatccc gtcgtaggta ttcgagacga gcttgcgatc
     6601 gaaatcggcc cccttgagca cggtgtcgac gagtgccagc cactgctctc gactgccgga
     6661 tgggaattct gcggcgagcg tcaactccga ggtggtcacc agattttctc caggtctgcg
     6721 gtatcgcacg ggatacgcaa ggttaccggg ggtgctcacg ccccgtcagg tgcgagaagt
     6781 tcggcaaggg actaggttcg accggcgcaa gcagccacgg ggtcctgcac ccctcaagca
     6841 cctggaacga tgatgttcgc taccgatgtc gctttttcgt cgatgaccga actgtcggtc
     6901 ggcctgttga tcctgcgggt cgcgttcggc ctcacgatgg ccgcacacgg atacgggaag
     6961 ttctttcagg gtgggcgtat ccccggcacc gccggatggt tcgactcgat gggcatgcgg
     7021 cccggcaagg tgcacgccgt gttggctgcg acgagtgagg ttgcttccgg cctcgccatc
     7081 gccctgggtc tactcacacc gtttgccggt ggtctgttca tcgccctgat gatcgtggcc
     7141 ggctacaccg tgcatgccga aaacggcttc ttcatcgtca agagtgggtg ggaatacaac
     7201 ttcatccttg cggtattcgg cctgggaatc gccatggtcg gcccgggtga gtattccctc
     7261 gaccacgcgt tcggcatcgc cgacaccttg tcgggctgga ccggtctgat catcgccctg
     7321 atcgtcggcg taggtggcgg catcggccaa ctcgtggtgt tctaccgccc tccggcgaag
     7381 acctcatagt ccggcacccc cggcgtctgc attccggtga atccggcgca gaattcttca
     7441 gatcgggcaa caccccttcc gaagccttct gtgcatgggg aataccgatc acgtgccatc
     7501 ggatcgcagg cgatgcgatg gtggcatcca cgacaccgac aatttgcgca ccaccctcgc
     7561 tcgtatcgcc gttcgccttg aggcgcagcg ttgcgagttg ttcgaggtcg acgagcaccg
     7621 cgcaacgctg ctggaccgtt gggtcgcgtc cggcatggcg attgcattgg ccgagcgccg
     7681 agatgtacca ctgcactggt ttccgtggtc cctcggctcg gtgcggccgc gcgagtacct
     7741 cttcgtacgc aatgccggtg cgctcccgat ggccctcgtt gaccatacgg agcgacccga
     7801 acaccgcgtc gtttctggtg ccgatctggg ggtcggtgct gcgctgatgg tgccgctcac
     7861 cttgaccggt catccgatcg gggcgctctg tgtgtactgg tcgctcgagc gtcagtcctg
     7921 ggagtccgag tcccgcgaca ccgtagcgtc gtgggcactc gatgccttgt tggccccgcg
     7981 ctgaaggcgt tcccagaagg cgccgccgcg gtgttcggtc ggcgggatgg catcgtcgct
     8041 gccactgctc gaaaccatcc gttgggtcag ttcgctttcg accaactcgt gtcggttaag
     8101 gaaagatgaa acgaaggcct ggatgattgc cgctgccggc agcgccaaca gcgctccgat
     8161 cggaccgatg atgctgccgc cggcgagcac cgcaccgaag gcgaccgccg ggtggatctc
     8221 catcgtgcgc gcggtgaccc gtggtgccag cacgtagttc tcgaactgct gatacacgat
     8281 catgaacacg aacaccgcca gcgcacgcgg tgggctgtcg agcaatgcga tcaccaccgg
     8341 cagcgcacca ccgatgtagg tcccgacgac cggaacgaac tgactcacga ttcccatcca
     8401 caccgcgagc gccagcgggg acggcacacc gatgatggcg aatgcgatcc aactgaggat
     8461 cgacgacacc accgccagga tcgtgcgcga gtacaggtaa ccgccggtct tggaaatcgc
     8521 cagctcccag agtccgagca ccatcacctg gcgatcctgt ggaaccaccg aacagatcgt
     8581 gcgccggaac tgcggtcctt ctgccaccaa atagaaggtg aagaggaaca ccgtgaacat
     8641 gttgaacacc acgccgagcg cggtgcccgt caccgacatg acccgaccgc cgacgtcgcc
     8701 ggcatacctc gtcaggtctc cttcgtactc gcggacctgc ttcacgagat cgtcggtcgt
     8761 gacattggcg tgaaaggtgt cgttgaacca acgggtcgcg tcttggatca gccgcggtgc
     8821 gtcgttgacc aacgaggtga cctgctgcac cacgagatcg acgatgaccc acacgagcac
     8881 gaacgaaccg acgagcaaca acaggaacac caggccggtg gcaagtccgc gcttcatccc
     8941 tctggccgag aggcggtcca ccgcaggctc gacagcaaag gacaagaaca gtgagaccaa
     9001 cagcagcacc aacaggtccg agagattggc gaataccgtt cggctcacca tgaatccgat
     9061 ggccccgagc atcacgtaga gcaccgcacg cgggaaccat cgcggcgggc gtgccgcgtt
     9121 ccacgtgtcg ggcaatccgt cgttcatcat caccgacgct gcatcggacg ccacgtcgga
     9181 cgccgagggg gccggagacg agatcgacag gtcggaggga tcgtcaccca ttgccgaaca
     9241 tcatcatcca ctgctcgacc gactggggcc gaaatacgaa gttctgggct cgggtctccc
     9301 ccatcggcgc cgacggcatc gccgagtacc gatggccggg atacaacacg gcgctgtcgg
     9361 ggaccttcgc aagccgctgg gtcaggctgt agtacaggtc ttcggcattt ccgcccggca
     9421 gatccgttcg cccgcaccct tcgagaaaca acgtgtcgcc ggaaacgaga tgatcgtgaa
     9481 ccaagaagca ttgcgatccg ggtgtgtgac caggggtgtg gatgagatcg acacgcacgt
     9541 cgccgatcat cacatgtgcc ccggtgcggt gaccgaccag gtcgggtgcg gtgactccag
     9601 tggtcttgga gatccattcg acctcggtct cctgcacatg aatcggcacc tgcacccggt
     9661 tcaacagcgc ctcgatcccc tccaggttga accccatcat cgacccgcca cagtggtcgg
     9721 cgtgatagtg cgtcaccagc gcaccggcga tcgtcatgtc gtccactgcc gccgaagcga
     9781 tgagaccatc gacgtcatag gcggggtcga cgacgagaca ctcgccggtc tcgcggtcgc
     9841 cgatcaggta cgagaagttc gccatctgcc cggcgatgac gtccgtctgg gcgaagtcgc
     9901 ggccggcgag gagttggcga aagtacagac ggtcgtcgct catgatgcgg aggctagacc
     9961 ggcaccgacc gcggcgagtg ccaggtcgat ctcgtcggct gagacgatca acggcgacat
    10021 gaaccggatg acgttgccgt cggtgcccgc gttcatcagc aggacccggt gctcgctgaa
    10081 ggcgtgggag atgaccgagg tgacggttgc ggcgtcgggt tcgccctcag cggtacggaa
    10141 ctcggccgcc accatgagcc cgggcccgcg gacgtcgacc aggttcgggt gtttcgcagc
    10201 caggccacga agccccgcgc gcaactgttc gccgcgggcg ttgacgttgt cgaggaaccc
    10261 ttcggcggtc acaacctcga tcgtcgccag tgcggcagca caaccgagtg ggttgccgcc
    10321 gtaggttccg ccgtgggttc cggtgatcca gcgatccatc agcgcccgag gcgacccgat
    10381 ggcggagatc ggaaagccgg aggcgaaccc cttcgccatg acgatgatgt cgggggtgac
    10441 cccggtttga tccatggcga acatggttcc ggtgcgaccg aacccggtct gaacctcatc
    10501 ggcgatgagc aacaggtcgt gttcgtcgca gatcgcacgg accccctgca catagcggac
    10561 cggagccggc tcatagccac cttcgccgag cacgggttcc aggatgatcg cggcggtctc
    10621 ctgcggtgcg gtttgtgtgg tgagcaaccg tcgcagttgc gccagtgccg catcgattcg
    10681 ttcgtcgagg gtgccgactc cgacggagcg tggaaagggg gtcacgtgaa ttcccgctgg
    10741 cagcggtgcg tagttgcggc gatatgcggt ctttgaggtc gtcatcgcca tcgccaggtg
    10801 ggtgcgcccg tgaaaggaag catccatgac gatgatgttc gatttacccg tcgcgtgctt
    10861 cgccagcttc accgccgcct ccacggcttc ggcaccggag ttggaaaaaa agaacgtgtc
    10921 gatacccgcc ggggtgagat cggcgaggcg agcggcaagc ggttcgagca gatcatggcg
    10981 atagcagttc acctgggcgt ggatgaagcg accggcctgg gttcggatcg cctcgaccac
    11041 ggttgggtgg caatgcccgg tcgatgtcac cgcgatgccc gccgtgaagt cgaggtaccg
    11101 ctctccggag gccgaggtga tccaacaccc ctcgcccgag accacatcga ggtccgtcag
    11161 gttggtccac accggtgaca gatgcgacac ggcgcacctc cttgattcga gtcgttgcgt
    11221 gccgcgctgg acggcgcgca tgtcacagca gtttggcgca cgcgccgagc gtgatgggct
    11281 cccgatagcc tcggcaccga tgagttctca ccagcctcgc accggcaccg tgtcgcccag
    11341 cgcctcggtc gggttcctcg gcccgatcgg caccttcacc gaacaggcgc tgctcggaca
    11401 gcccgacctt gccgccgcga cccgcatcga attcgacaac ttctcggagt tgctgttttc
    11461 ggtcgagaac ggcgaactgg actacggctt cgtcgcgttg gagaacgcca ttgaaggtca
    11521 ggtcaacatc accgccgaca ccttgacgtt cgacgtcgac ctgctcatcc aacgcgaggt
    11581 cgtgcttccg atcgagatga cgctgcaggt caaacccggt gcggtgctcg acaagatcac
    11641 gcgcgtttcg tcgtttcccc acgcctacgc gcaatgtcgg gaatggctgc gggccaacct
    11701 gccgaatgtc gagttggttc cggccacgtc gacggcgggc gcgagcgaga ttgtggcaac
    11761 gggcaacgat gcgaccatcg ccgcgatcgc caatgcccgt gcagccgaac tgcacggact
    11821 cgacatcatc gctcgagata tcgcggatca ccccgagaac gccacgagat tcgccttggt
    11881 cgctcgccgg ggaatccccg cgccctctgc caacgacaag acctcgatcg tcgtgttcca
    11941 acgcgctgac gttcccggtt cgctcttgag catcctgcag gaattcgccg cccgacagat
    12001 caacctgtcg ttgctgttat cgcggcccac caagaagtcg ctcggcgact actgctttct
    12061 catcgacatc gtgggccacc tcgccgaccc gctcgtcgcc gacgcgctcc ggtcgctcaa
    12121 gatgaaacac gacgtcaagt tcctgggttc gtatcccgcg ggagttgccc tcgacgatgc
    12181 cgaacgcgaa gccgccgagg cgcacctcga cgaggccaag gcatggatcc gctcgcttcg
    12241 agaacagttg gacgagtgac cgtttggtcg accagcggat gtgacgtcgg gggcgtaccc
    12301 actagagtct gcgccgtccg gagggatggc agagcggacg attgcgacgg tcttgaaaac
    12361 cgttgaggtg caagcctccg ggggttcgaa tccccctccc tccgccactg gcgggcgagt
    12421 tgtctccttt gcggggggcg acgtatacac tgttcgagcc tggagaggtg ccggagtccg
    12481 gtcgaacggg cctccctgct aaggaggtga cttgcgcaag tgagtccgtg ggttcgaatc
    12541 ccaccctctc cgccccacgc gagaaccttc aacgcccggg cccaacggtc cgggcgttgt
    12601 cgttgttcga catgacgag
//
LOCUS       DAOOMA010000011        12512 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_5574_length_12512_cov_4.355704, whole genome shotgun sequence.
ACCESSION   DAOOMA010000011 DAOOMA010000000
VERSION     DAOOMA010000011.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 12512)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12512)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12512
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5574_length_12512_cov_4.355704"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..558
                     /locus_tag="PLP95_00700"
     CDS             <1..558
                     /locus_tag="PLP95_00700"
                     /inference="COORDINATES: protein motif:HMM:NF012673.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0046855 - inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HPB44352.1"
                     /translation="EEFADKVGTSGRRWVVDPIDGTKAFTRGVATYTNLLYLEDATGP
                     RVGVINVPAIAEMVSAAVGLGAFFNGEPCRVNTTADPNASLLTTTGFDYWEPSMLEGV
                     RSSGMEMRTWGDGYGYALVATGRVEAMVDPVINFWDIAPCTVIIAEAGGRFSTTDGSD
                     DPSYPSFVATNGVIHDAVIDALNNR"
     gene            complement(561..1451)
                     /locus_tag="PLP95_00705"
     CDS             complement(561..1451)
                     /locus_tag="PLP95_00705"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FliA/WhiG family RNA polymerase sigma factor"
                     /protein_id="HPB44353.1"
                     /translation="MMLKATQSETVTRLIEDNLELVHHIVFQVAIHFPRHVDRDELAR
                     AGALGLVEAARRYDDDKGVPFQRFAAQRIRGAIIDAVRAADWAPRSVRSLSRKLEATE
                     QRLASHLGRLPNSGELAEELGMSRDELHRLRDRLFRSVVLAFDHLSTDNADEELTLVD
                     VIEDRHSVEPCGELERRELHAYLRDAVALLPERQRLVIVGYFLEERTSQELARFLGVT
                     ESRVSQMRTEALEELKRGIEAQYNRDTDEIDLANPRGRQSKRRAQFAEAIGSRQDFKS
                     RVGVAGAFDDESVLAELLAV"
     gene            complement(1679..2665)
                     /locus_tag="PLP95_00710"
     CDS             complement(1679..2665)
                     /locus_tag="PLP95_00710"
                     /inference="COORDINATES: protein motif:HMM:NF015412.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB44354.1"
                     /translation="MRIGVLGLGVVGQRLIELLDDDSVSTPVVVTGRVHKDREALRRI
                     HRDHVELVEFRIEADATFDVDVVVLASPDEHHATAASAALAGGAHVVSLADSAATVTE
                     LLSLNEFAREVGRSVVVGAAASPGVSTLLAIHAGTLFSRIDQVDIAVTGTAGPTCIDR
                     RARANRTETQEWRDAMWVDCGARSAPELVFFPDPVGSIECARGDLSESLVLRRVMPNV
                     DLLSIKVAKEPVSPVQRAGRRSRRPEREIEPGAQRIMVSGEIDGVPETVVYGLVASTR
                     DISADLAQLCAWAALSEQRSGAMTVGELVDAPSTLRMLADRGSQMLVYEDLD"
     gene            complement(2901..2974)
                     /locus_tag="PLP95_00715"
     tRNA            complement(2901..2974)
                     /locus_tag="PLP95_00715"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2938..2940),aa:Ile,seq:gat)
     gene            complement(3013..3405)
                     /locus_tag="PLP95_00720"
     CDS             complement(3013..3405)
                     /locus_tag="PLP95_00720"
                     /inference="COORDINATES: protein motif:HMM:NF023511.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3566 domain-containing protein"
                     /protein_id="HPB44355.1"
                     /translation="MTGRKRRFEARRVHRIIRHIDPWSVLKLSLVFFLCIWVIFMVAI
                     VIVWSIAQSSGTIGKIEDLLAELDITNSGFDGTFIFRQYGLFGLVMVFASTIATTVGS
                     IVFNLVSDLIGGIWITVIEEETSRPAAD"
     gene            complement(3829..4167)
                     /locus_tag="PLP95_00725"
     CDS             complement(3829..4167)
                     /locus_tag="PLP95_00725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB44356.1"
                     /translation="MTTSQRTTTAARPKRSERGSATLTMLAGLLMIGVAGAAVVMLAF
                     DGLERGRAQAVADLVALAAASEPGVGSSLAQRNGAILDSIELRGDVTVVVVRLGDSRA
                     IAAARLERAG"
     gene            complement(4118..6676)
                     /gene="gyrA"
                     /locus_tag="PLP95_00730"
     CDS             complement(4118..6676)
                     /gene="gyrA"
                     /locus_tag="PLP95_00730"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015439569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HPB44357.1"
                     /translation="MPDNDNENPTQQAEQPNEPIDSLVTHGSIEPIEIQTEMEQSFLD
                     YAMSVIVSRALPDVRDGLKPVHRRIFWAMHEGGLRPDRPHKKCATVVGDVLGKYHPHG
                     DQSVYHALVRMGQDFSVRHVLIDKHGNFGSPSDEPAAYRYTECRLSNLAMDLLANIDE
                     DTVDMVANFDGSTEEPTVLPSRFPNLLVTGSQGIAVGMATNIPPHNLGEVIDASLHLL
                     ENPEATSDDLMQFVKGPDFPTGGLIMGRAGILDAYRTGRGSVKIRAKAEIVEGPRGEQ
                     IVVSEIPYQTSVEQIETKIAELVNSREIEGIRAIRNGSAKGKIELVIELKKDAPALVI
                     LNNLYKHTPMQSSFAVNMVALVDGVPRTLSLRDVLHHYLQHQREVIRRRSEYRLDRAA
                     RRAHIVEGLLKALDMIDEIIAAIRASEDKAAARDALRNAPFEFSEQQAEYILDMQLSQ
                     LTRLGRTRLEEELAQLRETIAELEEILANDSKLRSVISTEMSEIRDKFADDRRSPITF
                     DPGDLDIEDLIEDEEIVVMMTAAGYIKTVSADAFRTQGRGGRGVAGTKLKDNDYVTDI
                     IHTSVHAYLLFFSNRGRVYRLKSYQIPMMERTARGTAIVNLLQLQPDEKIQTIIDTRD
                     YETNRYLFFVTRRGVGKKTLFNAYDNNRQAGLIAINLRDDDELVRVLPVNEGDEVMCV
                     SQLGRGIRFGEGDVRSMGRDATGVRVMRLKDSDEVVSADVVRPDHHLLTITDAGFGKR
                     TLPDAFTKQGRGGQGIRAMKINENKGTLVAGLMVADGDQLFLIADSGVTIRVPVDGIS
                     EQGRDATGVRVMNLDDKTHVVAVARVLASDADGDEGIDDDDEPTNDDGGQAEAE"
     gene            complement(6691..8634)
                     /gene="gyrB"
                     /locus_tag="PLP95_00735"
     CDS             complement(6691..8634)
                     /gene="gyrB"
                     /locus_tag="PLP95_00735"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012223523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009330 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) complex [Evidence
                     IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HPB44358.1"
                     /translation="MTQENGSYGADQIQILEGLEAVRKRPGMYIGSTGPAGLHHMVYE
                     VVDNSVDEAMAGFCRRIDVRLLPGGDGEGDGCEVIDDGRGIPVETMPGQDLSAAEVVL
                     TVLHAGGKFGGGGYKVSGGLHGVGVSVVNALSSRVEVEIVRDGGRHVMSFADGGVPVE
                     PLHRVGDAPDERTGTTVRFWPDGTIFDETTFRATTLLERFQMMAFLNAGLEITFRDDR
                     SPSPDADTTVYRYDGGIRDFVRHVNASKEALFSDVGYYAVAEETMEVEIAFQWNTGYN
                     TDGIHSFANGITTIEGGMHEEGFKKALTNCVNRYAKDKGLLKDKDPNLQGEDIREGMT
                     AIISVKLQDPQFEGQTKGKLGNVPMRSLVERATNEKLAEWLEEHPSEGKRIVQKALQA
                     SRAREAARNAREATRRKSALDGAGLPGKLADCSSNEPRECELYIVEGNSAGGSAKDAR
                     NPATMAILPIRGKILNVERARIDKMLKNQEVQSLVQAIGAGIGEEFDVDKARYHKVVM
                     LCDADVDGSHIRTLLLTFLFRQMRPLVEAGYVYIAQPPLYSTKVGRDTIYLKDDAAKA
                     AFLEDRPNHKEEFARLKGLGEMDADELWATTMDPARRTLLQVSLEQAVIADEVCSILM
                     GEDVESRKHFIQTNAHDVRFLDI"
     gene            complement(8798..9115)
                     /locus_tag="PLP95_00740"
     CDS             complement(8798..9115)
                     /locus_tag="PLP95_00740"
                     /inference="COORDINATES: protein motif:HMM:NF017103.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF721 domain-containing protein"
                     /protein_id="HPB44359.1"
                     /translation="MTSGGRPVSSVSSSIDALRRQFGLGRLSTYETITRQWPELVGEG
                     PAGESVVVELRNGILRVEAYDPAVAEVITWSRQALVQAIRQACPTEHVTDVSVRVRPR
                     PKP"
     gene            complement(9112..10206)
                     /locus_tag="PLP95_00745"
     CDS             complement(9112..10206)
                     /locus_tag="PLP95_00745"
                     /inference="COORDINATES: protein motif:HMM:TIGR00611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HPB44360.1"
                     /translation="MISVDRLHLRDFRSYEELDLEFAPGLTVVVGENGNGKTNLVEAI
                     GFLARLRSFRGAPNEALVRRGASEAIIRGDITCGERPVQIEAALPLSGRMRVQVNKQR
                     LVRRVDMAEYLTVTVFSPDDLEIVKGGPGLRRDFLDDLLSDLHPKNEVATSDFAKALR
                     QRNALLKQMAGRRDEASELTLDVWDLRLCELGERVAVLRSKTLAQLEPMVVEAYSELS
                     ARRGHAHSVQLRYGSTWFEGGLSGALAEARTADIRRGVSTVGPHRDDVELYIDAMPAR
                     THASQGEQRSLVLALRLAAHRLVTVARGQAPVLLLDDVFSELDTHRSAALLATLPQGQ
                     KILTSVGDVPPQATVESQLTIVDSTVRVSR"
     gene            complement(10211..11305)
                     /gene="dnaN"
                     /locus_tag="PLP95_00750"
     CDS             complement(10211..11305)
                     /gene="dnaN"
                     /locus_tag="PLP95_00750"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012223517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HPB44361.1"
                     /translation="MKFRCERDVLLDGLATAGRAVSSRGASLPVLSGLLLTLEGNNLR
                     LTGSDLDLTISVDLMVSGEVDGTIVMPAKLAVDIVKALPPGAVTIDTEGEEAKISSGR
                     SDFALNLIPAEEFPHFTTPAGEVVTIDAAAFTDGIRQVAAAASTDDSRPILTGVLMAA
                     EGSGLRLVSTDSYRLAVRDLPGSAVLEEGQSVLVPSRALQEVVRVLGGASELTLELGE
                     REASFSVEGTRLTTRLIDGEFPKYRSLIPQSQPNRLTIGRQELIEAVRRVKLMAREAT
                     PVRLTMRDGSAELAAITQDVGQAHESLDASFSGTELTVAFNPDYLLSGLEVTGGDEVS
                     LETVDSMKAALLRSNESTDFLYLLMPVRVS"
     gene            complement(11583..>12512)
                     /gene="dnaA"
                     /locus_tag="PLP95_00755"
     CDS             complement(11583..>12512)
                     /gene="dnaA"
                     /locus_tag="PLP95_00755"
                     /inference="COORDINATES: protein motif:HMM:TIGR00362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HPB44362.1"
                     /translation="ELLHVAGQVQLYGAAGLGKTHLLQAIAHYVNQNYPAYRVRYIST
                     ETLLNEFIDAIRHNKQAEFKRRYREIDVLLVDDVQFMEGKDQLQEEFFHTFNTLHQAN
                     RQIVLSSDRPPDAVATLEDRLRSRFKWGLVTDIQPPDFETRLAILRKKAEQGNVAIPS
                     SVLEFIVEHVTNNIRELEGALNRVTAYAALHQTDLTIEDAQRVLGDIVKSNQRQITPD
                     LIMETAAARFGFTVEELRAQDRRRPLVMARQIAMYLFRELTELSYPEIAKQFGGRDHT
                     TIIYGFDKIKALMKERHSVYDDVTSLINEIKGG"
BASE COUNT         2322 a         4040 c         3751 g         2399 t
ORIGIN      
        1 gaggagttcg ccgacaaggt cggaaccagt gggcgacgct gggtggtcga tccgatcgat
       61 ggcaccaagg cattcacccg gggtgtggcg acctacacca acctgctgta tctcgaagat
      121 gccaccgggc ccagggtcgg agttatcaac gttcccgcca tcgcggagat ggtgtcggcc
      181 gctgtcgggc tcggggcgtt cttcaacggt gaaccgtgtc gggtcaatac caccgccgac
      241 ccgaacgcat cgctgctcac caccaccggg ttcgactact gggaaccatc gatgctcgaa
      301 ggtgtgcgtt cgagcgggat ggaaatgcgc acctggggcg atggctacgg atacgcgctg
      361 gtggcgaccg gtcgggtgga ggccatggtc gatcccgtca tcaacttctg ggacatcgct
      421 ccgtgcaccg tgatcatcgc cgaagcgggc ggtcgtttca gcaccaccga tggctcggac
      481 gatccgagct atcccagctt cgttgccacc aacggcgtca tccacgacgc agtcatcgac
      541 gccctcaaca accgctgatt tcagacagca agcaactcgg caagtaccga ttcgtcgtcg
      601 aacgcaccgg cgacgccgac acgtgacttg aagtcctggc gcgacccgat ggcttcagcg
      661 aactgcgccc gacgcttcga ttgacgacct cgagggttcg ccaagtcgat ctcgtcggta
      721 tcacggttgt actgcgcctc gataccccgc ttgagttctt cgagggcctc ggtgcgcatc
      781 tggctgaccc gcgattcggt gacaccgagg aaccgagcga gctcctggct cgtgcgctcc
      841 tccaggaaat acccgacgat gaccagccgc tgccgttccg gcaacaaggc caccgcgtcg
      901 cgcagatacg cgtgcagttc gcgacgctcc agctcgccac acggctcaac cgaatggcgg
      961 tcctcgatta catccaccag ggtcagttcc tcgtccgcgt tgtcggtcga caggtgatcg
     1021 aaggccagca cgacggaacg gaacagtcga tcccgcaaac gatgcaactc gtcgcggctc
     1081 atgccgagtt cctcggccaa ttcgccgctg ttgggaagcc gaccgaggtg gctcgcgagg
     1141 cgttgttcgg tcgcctccag cttccgagac aagctgcgca ccgaccgggg agcccagtcg
     1201 gcggcgcgaa ccgcgtcgat gatcgcaccg cggatgcgtt gggcggcgaa tcgctggaac
     1261 ggcacaccct tgtcgtcgtc gtagcgtcgg gcggcttcga cgagtccgag ggctcccgcc
     1321 cgcgccaact cgtcacggtc gacgtggcga gggaagtgga tggcgacttg gaagacgatg
     1381 tggtgaacca actcgaggtt gtcctcgatg agtcgggtaa ccgtctcgga ttgtgtcgcc
     1441 ttcaacatca tgtgagtctc cccttcgcaa acaagctgaa ggaggtatcg gagcgcgccg
     1501 aagagaactt gagagatttc ttcgaaaaat attcagaccg aacccgactc cccagcggag
     1561 gacggaatcc acgtcacgat gatgatgcgg ttctatcggc cccaacgccg ctcacctgaa
     1621 gcatcttcga ggacttttct ccggacacgg atgcgaccgc gacccttggt caaggccctc
     1681 agtcgaggtc ttcgtagacc aacatctgcg acccgcgatc ggccagcatc cgcaaggtcg
     1741 agggtgcatc gaccagttcg ccgacggtca tcgctccgct tcgttgttca ctcaatgccg
     1801 cccacgcaca aagctgagcg aggtccgccg agatgtcgcg ggtcgacgcg acgagcccgt
     1861 agaccaccgt ctccggaacc ccatcgatct caccggagac catgatccgc tgagcgccgg
     1921 gttcgatctc gcgctcgggt ctgcgggagc gacgtccagc gcgttgaacc ggactcaccg
     1981 gctctttcgc aaccttgatc gacaacaggt cgacgttcgg catgaccctg cgaagtacca
     2041 gcgactcact caggtcgccg cgggcacatt cgatgctgcc gaccggatcg gggaagaaaa
     2101 ccaactccgg ggctgaacga gctccgcagt cgacccacat cgcgtcgcgc cactcttggg
     2161 tttcggtgcg attggcccga gcacgacgat cgatgcacgt gggacccgcc gtgccggtca
     2221 cggcgatatc cacctgatca atgcgtgaga agagcgtccc tgcatgaatt gccagcagcg
     2281 tcgacacgcc cggagaagcc gcggcgccga cgaccaccga ccttccaacc tctcgggcga
     2341 attcattgag cgatagcagc tccgtcacgg tcgccgcaga gtcggccaac gacaccacgt
     2401 gggccccgcc ggcaagtgct gcgctcgcag cggtggcgtg gtgttcatcg ggcgaggcca
     2461 aaaccacgac gtcgacgtcg aacgtggcgt cggcctcgat gcgaaactca accagctcta
     2521 catgatcccg atgaatccgc cgcagggctt ctcggtcctt gtgaaccctg cccgtcacca
     2581 ccaccggtgt cgacaccgag tcgtcgtcga gcaattcgat caaccgttgg cccacgacgc
     2641 cgaggccgag gacgccaatc ctcatcggaa gtcgtcaccg gtaagcggcc gccaaccacc
     2701 accgccggaa ggcgatgaat tcgccccgcg caagcgaatg atcgcagccg cgatcccgcc
     2761 caacaccatc gcaacagtgg ctcggcggat tgcttgcaac gactgctcct cgcctggttc
     2821 cgatgcgtac gaccgtttcg aacgcacctg ccgcggggct ggggcgccgt gacgactgaa
     2881 cgcctggctt gcggtgcctg tggggctagc tggaatcgaa ccagcgacct cacccttatc
     2941 aggggtgcgc tctaaccgac tgagctatag ccccaagcgg tgaaagattc tacaagacgc
     3001 cccaaggcgg cgctaatccg ccgctggacg actggtttcc tcttcgatca ccgtgatcca
     3061 gattccgccg atgagatcgg acaccaggtt gaacacgatg cttccgaccg tcgtggcgat
     3121 cgtcgacgca aacaccatga ccaacccgaa cagcccgtac tgacggaaaa tgaacgtgcc
     3181 gtcgaacccg ctgttggtga tatcgagttc cgcgagcagg tcttcgatct tgccgatcgt
     3241 tcctgagctc tgggcgatcg accacacgat gacgatcgcc accatgaata tcacccagat
     3301 acacaggaag aacaccaggg aaagcttcaa caccgaccag gggtcgatat ggcggatgat
     3361 tcgatggact cgccgggcct cgaaacgacg cttcctaccc gtcatggcgc gtcgagatac
     3421 cgtcgcctgt ggcactgtct cgatcgcgcc tcccgatgaa gcctccgagc gctgctgagc
     3481 acgaggtact gcgctcgggc gggcagttgg cgctacggcc ggctgctgag cggtcgatgg
     3541 ttggggtgca ccgacccgag ttgggcgctg ctgtgcactc cgctgctgtg gcgcgccctg
     3601 cgcggttgag ccctgcgcgg ttgacgcctg cggtccagtc ggtacggcac cggtgcgcgc
     3661 tgcgacgtcg ggcgccggga gatcgtgtcg tttcgtcatc ggagcggacg gcgtcgcttg
     3721 atggcgcgcg ttcgagtctg actgacccgg cgtgggagga ttcacgccga ggatcctacc
     3781 gttgtgcact gcccggtacg tgttcactcg cattgaaaca aggtgtgctc acccggctcg
     3841 ctcaagtcgg gcagccgcaa tcgcacgcga gtcaccgaga cgcacgacca ccacggtgac
     3901 gtctccacga agctcgatgg aatccagaat cgcaccattg cgttgcgcca acgaggagcc
     3961 gactcccggt tcggaggccg ccgccagggc aaccaagtcc gcgaccgctt gggcccggcc
     4021 acgctcgagc ccatcgaacg ccaacatcac caccgcggcg ccggcaacgc cgatcatgag
     4081 gagtccggcg agcatggtca gcgtcgccga accccgttca ctccgcttcg gcctggccgc
     4141 cgtcgtcgtt cgttggctcg tcgtcatcgt cgatcccctc atcgccgtcg gcgtcggatg
     4201 cgaggacgcg agctaccgcc acgacatggg tcttgtcgtc gaggttcatt acccgaacac
     4261 cggtcgcgtc gcgaccttgt tcggagatgc catccaccgg aacccggatg gtgacacccg
     4321 agtcggcaat caggaacagt tggtcgccgt cggccaccat caatccagca acgagcgttc
     4381 ccttgttctc gttgatcttc atcgcccgga taccctggcc gccacgtccc tgtttggtga
     4441 acgcatcggg cagggttcgc ttgccgaatc cggcatcggt gatcgtcaac agatggtgat
     4501 cggggcgaac cacatcggcc gagaccacct cgtcggagtc tttcagtctc atcacccgaa
     4561 caccggtggc gtcacgaccc atcgatctga cgtcgccctc cccgaaacga ataccgcgcc
     4621 cgagttgtga cacgcacatc acctcgtcgc cctcgttgac gggcagaacc cgaaccagtt
     4681 catcgtcgtc acgaaggttg atggcaatca accccgcctg gcggttgttg tcgtaggcgt
     4741 tgaacagggt tttcttgccg acgccgcgcc gggtcacaaa gaacaagtac cggttggttt
     4801 cgtagtcgcg ggtgtcgatg atcgtttgga tcttctcgtc cggctggagt tgcaacaggt
     4861 tgacgatcgc tgtgcctcgg gcagtgcgct ccatcatcgg gatctgatac gacttcaacc
     4921 gatacacacg gccgcggttc gaaaagaaca acaagtacgc atggacgctg gtgtgaatga
     4981 tgtcggtgac gtagtcgttg tccttcagct tcgttcccgc caccccgcgg ccgccgcgac
     5041 cctgggtccg gaacgcgtcc gcggaaacgg tcttgatgta gcccgcggcg gtcatcatca
     5101 ccacgatctc ttcgtcctcg atgagatctt cgatgtcgag atcgcccgga tcgaaggtga
     5161 tcggactacg gcggtcatcg gcgaacttgt cgcgaatctc gctcatctcc gtcgaaatca
     5221 ccgaccgcag ctttgaatcg ttggccagga tctcctccaa ctccgcgatc gtctcgcgca
     5281 gttgggccag ctcttcctct aggcgggtgc gacccaaccg ggtgagctgg ctgagttgca
     5341 tgtccaagat gtactcggcc tgctgttcgg agaactcgaa cggtgcgttt cgcagcgcat
     5401 cgcgcgccgc cgccttatcc tccgatgccc ggatcgccgc aatgatctca tcgatcatgt
     5461 cgagggcctt caggagtccc tcgacgatgt gagcccgacg cgccgcacgg tcgaggcggt
     5521 actccgaccg tcgccggatg acctcgcgct gatgttgcag gtagtggtgc aacacgtcgc
     5581 gcagcgaaag cgtgcgtggg acgccatcga caagcgccac catgttcacc gcaaagctcg
     5641 actgcatcgg cgtgtgcttg tagaggttgt tgaggatgac gagcgccggt gcgtccttct
     5701 tcagttcgat gacgagttcg atcttgccct tcgctgatcc gttacggatc gctcggatgc
     5761 cttcgatctc gcgactgttc accagttccg cgatcttggt ctcgatctgt tcaaccgagg
     5821 tctggtacgg aatctcactc acgacgatct gctcgccgcg cggaccttca acgatttcag
     5881 ccttcgctcg gatcttcacc gacccacggc ctgtgcgata ggcgtcgagg atgccggcac
     5941 gacccatgat gagcccgccg gtagggaaat ccggcccctt cacgaactgc atcaggtcgt
     6001 cggaagtcgc ttcagggttc tcgagaaggt gcaacgacgc gtcgatcact tcgccgagat
     6061 tgtgaggcgg aatgttcgtc gccatgccga ccgcgatgcc ttgtgatccg gtgaccagca
     6121 ggttcgggaa ccggctcggc aagaccgtcg gctcctcggt ggagccgtcg aagttcgcca
     6181 ccatgtcgac ggtgtcttcg tcgatgttgg ccagcaggtc catggccagg ttcgacagcc
     6241 gacactcggt gtagcgatac gccgcgggct catcggaggg cgaaccgaag ttgccgtgct
     6301 tgtcgatgag cacgtgccga acagagaaat cctgacccat gcgaaccagc gcgtggtaca
     6361 ccgactgatc tccgtgaggg tgatacttgc caagcacgtc accgacgacg gtggcgcatt
     6421 tcttatgggg tcggtcggga cgaagccctc cctcgtgcat cgcccagaaa attcttcggt
     6481 gcaccggctt gagcccatcg cgaacgtccg gcagcgctcg gctgacgatg accgacatgg
     6541 cgtagtcgag aaacgactgc tccatctcgg tttggatttc gatcggttcg atcgagccgt
     6601 gtgtcacgag gctgtcgatc ggctcgttgg gctgttcggc ttgctgggtg gggttctcgt
     6661 tgtcgttgtc aggcatgtcg attcagtccg tcagatgtcg agaaagcgca cgtcatgggc
     6721 gttggtctgg atgaagtgct tgcgcgattc gacgtcttca cccatcagga tcgagcacac
     6781 ctcgtctgcg atcacggcct gttcgagact cacctgcaac agcgttcgcc gagccgggtc
     6841 catggtggtc gcccagagct catcggcatc catctctcca agacccttga ggcgagcgaa
     6901 ctcttccttg tggttgggcc ggtcctcgag gaacgccgcc ttggctgcgt cgtctttgag
     6961 atagatggtg tcgcgaccga ccttggttga atacaacggc ggctgtgcga tgtagacata
     7021 cccggcttcg acgaggggtc gcatctgtcg aaacaggaac gtcaacaaca gtgtccggat
     7081 gtgactgccg tcgacgtccg cgtcacacaa catgacgacc ttgtggtagc gggccttgtc
     7141 gacatcgaat tcctcaccga tgcctgcccc gatcgcctga accagcgact gcacttcctg
     7201 gttcttgagc atcttgtcga tgcgtgcccg ttcgacgttg aggatcttgc cgcggatcgg
     7261 cagaatcgcc atcgtcgccg ggttgcgggc gtccttggcg gaaccaccag ccgagttgcc
     7321 ctcgacgatg tacagctcgc actcccgggg ctcattcgac gagcagtcgg ccaacttgcc
     7381 gggcagcccc gccccgtcga gcgccgactt acgccgcgtc gcttcgcggg cgttgcgggc
     7441 cgcttcacgc gcacgcgagg cctggagagc cttctgcacg attcgcttgc cctccgaagg
     7501 gtgttcctca agccattccg caagcttctc gttcgttgcc cgctcgacga gtgatcgcat
     7561 cggcacgttg ccgagcttgc ccttcgtctg accctcgaac tgcgggtcct ggagtttcac
     7621 cgaaatgatc gccgtcatcc cctcgcggat gtcctcacct tggagattgg ggtccttgtc
     7681 tttgaggaga cccttgtcct ttgcgtagcg attcacgcag ttggtgagcg ccttcttgaa
     7741 gccctcttcg tgcatgccgc cttcgatcgt cgtgatgccg ttcgcgaagc tgtggatgcc
     7801 gtcggtgttg tagcccgtgt tccactggaa cgcgatttcg acctccatcg tctcctcggc
     7861 gaccgcgtag tagccgacgt cggagaacaa ggcttccttc gacgcgttca cgtggcggac
     7921 gaagtcgcga attccaccgt catagcgata caccgtcgtg tccgcgtccg ggcttggtga
     7981 gcgatcgtcg cggaaggtga tctccaggcc ggcgttcaga aacgccatca tctgaaatcg
     8041 ctcaagaagc gtggtggcgc gaaatgtcgt ttcgtcaaaa atggtgccgt cgggccagaa
     8101 acgtacggtg gtgccggttc gctcatcggg cgcgtcaccg actcgatgca acggctcgac
     8161 agggacaccg ccgtcggcaa acgacatcac atgccgccca ccgtctcgga cgatctccac
     8221 ctcgacgcgc gagctcaacg cgttcaccac cgaaacgcca accccgtgca ggccaccgga
     8281 gaccttgtag ccgcctccac cgaactttcc gccggcgtgg agcacggtca acacgacttc
     8341 cgcggccgac aggtcctgtc ccggcatggt ttcgaccggg atgccgcgcc cgtcgtcgat
     8401 cacttcgcag ccgtcgcctt cgccgtcgcc ccccggcaac aaccgcacat cgattcgccg
     8461 gcaaaagccc gccatcgcct cgtcgacgga gttgtcgacg acctcataca ccatgtggtg
     8521 caggccagcc ggacccgtcg aaccgatgta catgcccgga cgtttccgga cggcttccaa
     8581 tccttcgagg atctggatct gatcagcccc gtaggagccg ttttcctggg tcacgcagcg
     8641 ccctttccga ccgttctcga tcgcggaacc cgcccgaagc gcctgccgat gccgggacgc
     8701 cgatccttct tctcagcacg aggatccgcg ggttcaatgt gcacgacgta attgtaccat
     8761 ttccagggct tggccctggc cgcaagaccc ccctagctca gggcttcgga cgtggcctca
     8821 cgcgtaccga tacatcggtt acgtgctccg ttggacacgc ctgccgtatc gcttgaacga
     8881 gcgcctgtcg cgaccacgtg atgacctcgg ccaccgccgg gtcgtaggcc tcaacgcgaa
     8941 gaatgccgtt gcgcagttcc accacgaccg actcgcctgc cggaccttcg ccgacgagtt
     9001 ccggccactg acgggtgatc gtctcgtagg tcgacagccg acccaagccg aactgacgcc
     9061 gcaaggcatc gatgctcgac gacaccgaac tgacaggtct acctccactg gtcatcgcga
     9121 aaccctaacg gtggaatcga cgatggtcag ctgcgactca accgtggcct gcggcggaac
     9181 gtcaccgacc gaggtcagga tcttctgacc ttgcggcaac gtggcgagca gcgccgctga
     9241 acgatgcgtg tcgagttccg agaacacgtc gtccaacaac agcaccggcg cctgtccacg
     9301 agcgacggtc accagccgat gggccgctag ccgcaacgcc aacaccagcg agcgctgttc
     9361 cccttgcgac gcatgggttc gcgctggcat ggcgtcgatg tacagctcga cgtcgtctcg
     9421 atgcgggccg accgtcgaca cgccacggcg aatatcagcg gttcgggcct cagcgagcgc
     9481 accgctgaga ccaccctcga accacgtcga cccgtagcgc aattgcaccg aatgtgcgtg
     9541 accacgtcgt gcggacaact ccgagtacgc ctcaacgacc atcggttcga gttgcgccag
     9601 ggtcttggaa cgcaacaccg cgacgcgctc gccaagttcg cagaggcgca gatcccacac
     9661 gtcgagggtc agttcactcg cttcatcgcg acgtccagcc atctgcttga gtaaggcgtt
     9721 gcgttgccgc aacgccttcg cgaagtcgct cgtggcaacc tcgtttttcg ggtgcagatc
     9781 cgacaggaga tcatcgagga aatcccggcg cagaccaggg ccgcccttga cgatttctag
     9841 atcgtcggga gaaaacaccg tgaccgtcag atactcagcc atatcgacac gtcgaacgag
     9901 tcgctgctta ttgacctgaa cccgcatcct cccgctgagg ggcagcgccg cctcgatctg
     9961 aaccgggcgt tcgccacagg tgatgtcgcc acgaatgatg gcctcggagg caccgcggcg
    10021 caccagggcc tcattcggcg ctccgcgaaa cgaccgaagg cgggcgagaa acccgatcgc
    10081 ctcgacgagg ttggtcttgc cgttgccgtt ctcgccgacg acgaccgtca ggcccggagc
    10141 gaactcgaga tcgagttcct cgtatgaccg aaagtcccgc aggtgcaatc ggtcgaccga
    10201 gatcaccgaa ctacgagacc cgcaccggca tgagcaggta caggaaatcg gtactttcgt
    10261 tcgaccgcaa cagcgccgcc ttcatcgaat cgaccgtttc cagcgaaacc tcgtcgcctc
    10321 cggtcacctc caacccggac aacagataat cggggttgaa tgccaccgtg agttctgtcc
    10381 cggagaaact tgcatcgagc gactcgtgcg cctgaccgac gtcctgggtg atggccgcga
    10441 gttcggccga cccgtcgcgc atcgtcaatc gaaccggtgt cgcctcgcgg gccatcagct
    10501 tcacgcgacg caccgcttcg atgagttcct gacggccgat tgtcaaccgg ttcggctggc
    10561 tctggggaat caggctccga tacttcggga actcaccgtc gatgagtcgg gtggtcaggc
    10621 gggtgccttc gaccgagaag ctggcctctc gctcgccgag ttcgagcgtc aactcgctgg
    10681 ctccaccgag tacccgcacg acctcctgca aggctcgcga gggaaccagc acactctggc
    10741 cctcctcgag caccgccgag ccggggaggt cacgcaccgc gagccgatag gaatccgtcg
    10801 acaccagtcg taggccggat ccttcggccg ccatcaacac gccggtcagg atcggacggg
    10861 aatcgtcggt cgacgcggcg gccgccactt gacggatccc gtcggtgaac gcggctgcat
    10921 caatcgtcac cacttcaccc gccggggttg tgaagtgcgg gaattcctcg gcaggaatca
    10981 gattcagagc gaagtcggag cgaccactgc tgatcttcgc ttcctcaccc tcggtatcga
    11041 tggtgacggc ccctggcggc agcgccttga cgatgtccac cgccagcttc gccggcatga
    11101 cgatggtgcc gtcgacctca cccgagacca tcaggtcgac cgagatcgtc agatcgaggt
    11161 cacttcccgt caggcgcagg ttgttgccct caagggtcaa cagcaggccc gacagaaccg
    11221 gtagggacgc gcctcgactc gaaaccgcac gaccggctgt ggcaagtccg tcgaggagga
    11281 cgtcacgttc gcagcggaat ttcacagtgt ctgcttcttt ctacccatcg atgtgtatca
    11341 ctaggcacta gtagtagtag ggcctgtgga ttgtggaaaa cctagtccgc ggcccgaaat
    11401 ttcgggagtt cgtcgtccag aggaagttcg cgtccgtccc cagacaagcg tgtaatttca
    11461 cgaagtgttg gggaggacca tgggttcctc ccatgctcgt ccaccctcag gccccagctt
    11521 catccaccgg tctcgtgaaa ccacacgaga cgcgaccgac ggtgacgttg ggactcggaa
    11581 cactagccac ccttgatctc gttgatcagc gaggtcacat cgtcgtagac ggagtggcgt
    11641 tctttcatca gggccttgat cttgtcgaag ccgtagatga tggtcgtgtg atcgcgtccg
    11701 ccgaactgct tggcgatctc gggatagctg agttcggtca gttcgcggaa cagatacatc
    11761 gcaatctgtc gggccatcac cagcggacgt cgacgatcct gggctcgaag ctcctcgacg
    11821 gtgaaaccga aacgagcagc ggccgtctcc atgatgagat ccggagtgat ctggcgctgg
    11881 ttcgatttca cgatgtcgcc gagcacccgc tgagcatcct cgatcgtgag atcggtctgg
    11941 tgcagcgccg cgtaggcggt cacgcggttc aacgcgcctt cgagttcgcg aatgttgttc
    12001 gtcacgtgtt cgacgatgaa ctcgagcacc gacgacggaa tcgccacgtt gccctgctcg
    12061 gccttcttgc gaaggatcgc aagcctcgtt tcgaagtccg gcggctggat gtcggtgacg
    12121 aggccccatt tgaagcgact tcgcagtcgg tcctcaaggg tcgccacggc atcgggtggg
    12181 cgatccgaac tcagcacgat ctgacggttc gcctggtgca gggtgttgaa ggtgtgaaag
    12241 aactcctcct ggagttggtc cttgccttcc atgaactgca cgtcgtcgac gaggagcacg
    12301 tcgatctcgc gatatcgacg tttgaattcc gcctgcttgt tgtggcggat ggcgtcgatg
    12361 aactcgttca gcagcgtttc ggtggagatg taccgcaccc gataggccgg gtagttctga
    12421 ttgacgtagt gggcgatcgc ctgcaggagg tgggtcttgc cgagcccggc cgcaccgtag
    12481 agctgcacct gaccggccac atggagaagt tc
//
LOCUS       DAOOMA010000012        12314 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_5682_length_12314_cov_3.982870, whole genome shotgun sequence.
ACCESSION   DAOOMA010000012 DAOOMA010000000
VERSION     DAOOMA010000012.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 12314)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12314)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12314
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5682_length_12314_cov_3.982870"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..191)
                     /locus_tag="PLP95_00760"
     CDS             complement(<1..191)
                     /locus_tag="PLP95_00760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB44363.1"
                     /translation="MSTEKRESRSLGLIIGAFTLVYLIGIGVIVLATAAWGGGGSGSG
                     SGSGSDESILNISLGEFAI"
     gene            complement(286..933)
                     /locus_tag="PLP95_00765"
     CDS             complement(286..933)
                     /locus_tag="PLP95_00765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020378189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HPB44364.1"
                     /translation="MGSRIFLVDDHEIVRRGLRDLFNRESDLEVVGEAATVADALARI
                     PLSNADVAVLDLGLPDGSGIEICRELRSSAPRMHSIILTSYDDEESLIEAALAGASGY
                     ALKQLKGNEIVAAVRAAAQGRSTMNPDVTARSLERVRADREANEPLESLTDQEREILE
                     LIAMGKSNREIAEHMYLAEKTIKNRVTALFAKLGVSRRAQAAVYAARIMERYGTL"
     gene            1011..2144
                     /locus_tag="PLP95_00770"
     CDS             1011..2144
                     /locus_tag="PLP95_00770"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing sensor histidine kinase"
                     /protein_id="HPB44365.1"
                     /translation="MQGSLGRENQRRLLDAIIHIGSELDLAPVLRRIVEVATELVDAN
                     YGALGVLDETRTHLAEFHTVGMPDDQVDRIGEPPKGHGILGVLIVDPRPLRLPDLTKH
                     PDSFGFPLHHPPMTSLLGVPIIVRGEAFGNLYLCDKRDGGVFSSEDEVLVVALASAAA
                     MAIDNARLHSRVCDLARNEDRERIARDLHDTVIQRVFAVGLGLQQAMLIDDLDEVRER
                     VDTAIDELDETAREVRSAIFELKAVERSTRTLRELIASTAAEAARVLGFEPTVTLTGP
                     VNAGVSEERAVHVIAVIREALSNVARHAEATAVTITLSVGDGHLSLTVVDDGVGVTPG
                     EIGGQGLSNMEGRAARFGGSMLVTPATPQGSGTRVTWSIPLED"
     gene            complement(2193..3041)
                     /locus_tag="PLP95_00775"
     CDS             complement(2193..3041)
                     /locus_tag="PLP95_00775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HPB44366.1"
                     /translation="MLNAAALVGPSERVIDLSDFARPKPRSRSRFPGDPTISVVYGGG
                     GAMGIAWHVAVIDALRSLGLPVDAAPSIGTSAGSWACGAARLGLSFDEFAGFGDLDLP
                     DRSPGLLRGLAAQIYGEARLDNVRISVTALPTMRHHLLDASRFPLPDLIAASSAVPGV
                     FSPHEIAGRRYVDGGVRAMASAGSAAGADLLIVSLPIAGAVFGPVGRAFEQAARRAMA
                     RWRERHGGTTIVLRPGAAFASRIGVRPGNVMDVELAKSLYPVAYDTVVRRLETRIRQI
                     ARSTSH"
     gene            complement(3147..4199)
                     /locus_tag="PLP95_00780"
     CDS             complement(3147..4199)
                     /locus_tag="PLP95_00780"
                     /inference="COORDINATES: protein motif:HMM:NF015338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HPB44367.1"
                     /translation="MTRTTRWTTAALATAVATALLFASGCSGDDDSASGDTSAATPST
                     TTSTIEEGAEEVAEEGASGSFTLLSYNVAGLPAEISSVDPAEHLPLISPLLNDYDIVA
                     TQEDFDWWGPLAANFDFVNYHDRLRADATHEFRTEQHPGPEAVGLDLAARMEPFIGDG
                     LGVLSRLPIANTRRVPWDGCFGGLDTSDGGAADCAAMKGFLMTEVTLPSGDLVHLYDL
                     HGEAGGSPTDQDLQVADYRQLATFIAEHSTDAAVIVAGDTNLHTYSDHEDASDGADTR
                     IWEEFLASTGLTDSCDATSCAERDQIDKVAVRSGATVELRVESHRFVSDRFVDAAGEP
                     LSDHPPLSVRIGWKIR"
     gene            4274..5278
                     /locus_tag="PLP95_00785"
     CDS             4274..5278
                     /locus_tag="PLP95_00785"
                     /inference="COORDINATES: protein motif:HMM:NF038209.1"
                     /note="Built with help from friend_finder, bracket5, and
                     grep mycofactocin; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mycofactocin-associated electron transfer
                     flavoprotein alpha subunit"
                     /protein_id="HPB44368.1"
                     /translation="MSGRPLVVVPVRGGVLPAGGLESIAEAQAAGDARIVLIGDGLEA
                     VIADLASIADSVETLEAGAFAVTAWSALLAPLAAEASFVLLPGSPDGRDLAVRLGARI
                     ECDVVTGALAVSADAVTVSHHGGLVQTALRPTGRFIATLQPNVRGVDAHGGPDTEESE
                     ATVVSLAVQVEPLATARDAAVVRVEAPEASAMDLAESARIVGAGAGLMADEGQADALR
                     ARFERLRAVGASLGASMGATRVVTDAGWVGHDRQIGTTGVVVDPELYVAFGVSGAVQH
                     TAGLGHPDHIISVNLDPHCPMMAMADLAVVADGAATLDAIAELLHVGDGSAQEVTTDG
                     "
     gene            5271..6569
                     /locus_tag="PLP95_00790"
     CDS             5271..6569
                     /locus_tag="PLP95_00790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020378763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HPB44369.1"
                     /translation="MVDVDAIVVGAGPAGAAAAIALARAGRSVVLLERGPFPGSKNMY
                     GGVIYGRILDGIVANWYDEAPIQRWITRRSTMMMTGHQALTIDYRTSTWGEAPYNGCT
                     AYRPDFDNWLAGHATDAGAELICSTTATGLLREGGRVVGVRTDRPDGDLTAKVVIACD
                     GVNSFLAKEAGMYHHTEAEHFTVGVKETIHLGSDVIDQRFALRGREGADFEIVGCTGG
                     IPGGGFLYTNLDTVAIGLVLQLPGLGAQDKRPEQLIADLKSHPSIAPLVEGGEVKEYS
                     AHLIPEGGYNMMPDLVTDGMLVAGDAAAMCLAAGIWLEGVNFAIASGFAAGQSAVDAL
                     RRGDTSADGLSRYRHRLETSFVLADHLKLRAVPDLMMNERVQQRYPQLACNVAERIFT
                     VENPRPKPGFNTIVRSELKASGLKMREVLKDAVTGLRSFG"
     gene            6566..6871
                     /locus_tag="PLP95_00795"
     CDS             6566..6871
                     /locus_tag="PLP95_00795"
                     /inference="COORDINATES: protein motif:HMM:NF024585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HPB44370.1"
                     /translation="MNASEDNYLDISFEHRMGLVEFRVAETAHITVDGDACRGCTTMQ
                     CVTACPANLFIPTSDGGILFNYEQCFECGTCYMVCNTEGAISWSYPTGGHGVVFSKS"
     gene            6984..7865
                     /locus_tag="PLP95_00800"
     CDS             6984..7865
                     /locus_tag="PLP95_00800"
                     /inference="COORDINATES: protein motif:HMM:TIGR04503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mycofactocin-associated electron transfer
                     flavoprotein beta subunit"
                     /protein_id="HPB44371.1"
                     /translation="MNWVDLHPEIDLIDGSISTDRRRYGFSEADRAALEVGLGIADRD
                     RGDGCDVEVVVMSVAPSAADDSLIELAASGADRIVRIDGDPDGDPALSAAAVGRALAH
                     GIAAIGDVSLVVCGDLGFAWGSGAVPGYIAHHLEVPQALGALEVSAAVEAVGVAEVGP
                     GELSVLRRLDGGRRELLRVPRPAVVSVEGAAARLRRAPLRAVMELRRRGVRVDSVALP
                     MPSGLLAEHVGPIRPRARVLAPPGATRALDRIAELTGAFVDRNPPRTVEAEPAEAAAL
                     IVEQLRTWGYLTDDPIP"
     gene            7862..8551
                     /gene="mftE"
                     /locus_tag="PLP95_00805"
     CDS             7862..8551
                     /gene="mftE"
                     /locus_tag="PLP95_00805"
                     /inference="COORDINATES: protein motif:HMM:NF014670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0140604 - mycofactocin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mycofactocin biosynthesis peptidyl-dipeptidase
                     MftE"
                     /protein_id="HPB44372.1"
                     /translation="MTARQLGDATWPEIAQRLSTLVIPLGSTEQHGPHLPLDTDTRIA
                     VACATRLVEARPDLVLGPAMAIGASGEHAGFAGTLSMGAEVFEAAIVEIGRSADAFDG
                     VIWVNAHGGNSVALRSAHATLVSESRRSLVLRCAVPGGDSHAGRTETSMMLSIAPQVV
                     RSDLAAPGRSEPWAELSEAILAGGVVAVSDNGVLGDPAGATAEEGKRFLDGIHAQFLD
                     AVARWIDGDSS"
     gene            8535..9374
                     /locus_tag="PLP95_00810"
     CDS             8535..9374
                     /locus_tag="PLP95_00810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012226322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mycofactocin-coupled SDR family oxidoreductase"
                     /protein_id="HPB44373.1"
                     /translation="MATPPEPQNPTGAGPDGGAIVGVALVTGAARGIGAATVRRLLSQ
                     GWGVVATDICGNIDGLGYPMGTLKELRAVVDGSGGRGVAVVADVRDRRALDAAVEIAL
                     DRFGRLDAAIACAGVMLGATHGWEMTPRAFDLCVDINLGGVQRCASAALPAIIESATP
                     GAGRFVAVASAAASSGRPQIAAYSAAKAGVIGLVRSLAAELAPLGVTANCVSPGSTDT
                     TILAASAALYGLADPAEFVAHQPVGRLLDPDEVAAGIAWLCSPQRGGVTGIVLPIDGG
                     MSL"
     gene            9371..10804
                     /gene="mftF"
                     /locus_tag="PLP95_00815"
     CDS             9371..10804
                     /gene="mftF"
                     /locus_tag="PLP95_00815"
                     /inference="COORDINATES: protein motif:HMM:TIGR03965.1"
                     /note="Members of this protein family, MftF, are
                     glycosyltransferases, members of PF00535 (glycosyl
                     transferase family 2). The encoding gene is found as part
                     of the mycofactocin cassette, in Mycobacterium
                     tuberculosis, many other Actinobacteria, and occasional
                     members of other lineages. Mycofactocin itself, a putative
                     redox carrier, is a heavily modified derivative of the
                     C-terminal Val-Tyr dipeptide of the mycofactocin precursor
                     MftA (TIGR03969); Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0140604 - mycofactocin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mycofactocin biosynthesis glycosyltransferase
                     MftF"
                     /protein_id="HPB44374.1"
                     /translation="MTTRRYRLAGSVRSVAGGRGLLGGDPIRLMRLSVAGSRLARSWG
                     DDGIVVGSGSEAADALAERLVDAGMAVPVPALLTPAELAAALEDLTVIVPVFERVDAL
                     DRCLKALRNSGVRNIVVCDDASPNGDEIREVCTRHDARVVRRGANGGPAAARMTGWVS
                     LERPDVGFIAFVDSDVEVSPSWLHPLLGLFGDERVALAAPRVAHLETDGGVLDRYEEA
                     NSPLDLGGRQAAISAGTRVSYVPAAALVVSGAAFSQVDGFDRSLSVGEDVDLCWRLVD
                     SGWRCRYEPASVVEHRGRSSLGAMLARRIDYGRSAAQLDRRHRGALPPLRANVWSLAA
                     VAALGARRPGIAVALTAWAVRSMSKRLDDIEPRIVARLVLSGDAGAGRQLARALVRPW
                     LPVTLVAGAASRRIRRVAMIAVAVDLLWRWAERSPRLDPVRFGALCLLDDAAYTLGVW
                     IGCVREGRIGVLLPEISVAAPNSAGKK"
     gene            10872..11330
                     /locus_tag="PLP95_00820"
     CDS             10872..11330
                     /locus_tag="PLP95_00820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020378497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold domain-containing protein"
                     /protein_id="HPB44375.1"
                     /translation="MGNAAGSSDLLRADLVLEYPFNRTTGPVIGAFYTGLREQVVVGI
                     RDADGRVVCPPVEYDPTTGAPLTELVKVGDTGTITSWTWVDNPREVEALDRPYALALI
                     QLDGADTAMLQVVDAPGPDALSTGARVKIRWAAERSGAITDIEAFELVEG"
     gene            11332..11907
                     /locus_tag="PLP95_00825"
     CDS             11332..11907
                     /locus_tag="PLP95_00825"
                     /inference="COORDINATES: protein motif:HMM:NF013920.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold domain-containing protein"
                     /protein_id="HPB44376.1"
                     /translation="MAAESNIAELPAGLWAQPAIDTSAVGEVVENDPVQRVRLPASIR
                     YDFVPGVATTRFLKGLKEKKILGEREADSDDVYVPPRGMSPTSGKPTTVQVELPHKGS
                     IASYCVVHIGFGVNAPPTPFVSALVLLDGACISIYGPVQEIDYDKVRIGMRVEPVWVD
                     DDQLDTSFESIKYWRPIDEPDVPAESLKGHM"
     gene            11907..>12314
                     /locus_tag="PLP95_00830"
     CDS             11907..>12314
                     /locus_tag="PLP95_00830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020378495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-transfer protein"
                     /protein_id="HPB44377.1"
                     /translation="MAAEFAAPSRDVAIVSVAQSKHERAIVERNEVELIQPVIQQAME
                     QIGAHISEIDFVCSGSSDYLAGQAFSFVMTLDAVGAWPPKSESHVEMDGAWALYEAWL
                     KIQAGHADTALVYGYGKSSPGDLPSVLSRKLDPY"
BASE COUNT         1924 a         3953 c         4304 g         2133 t
ORIGIN      
        1 tagatcgcga actcgccgag cgagatgttg aggattgact catcgctgcc cgaacccgaa
       61 cccgaacccg aaccgccacc gccccaagcg gcggtcgcga gaacgatcac tccgataccg
      121 atgaggtaga cgagcgtgaa cgcgccaatg atcagcccga gagaccggct ctcacgtttc
      181 tccgttgaca tacgtcctcc acagatgtct ggtagttgag gcacatcttt acagggtcga
      241 tggcttgcgg gcaaggaccg aagtcccccg ggtgcgtgac catcttcaca aggtgccgta
      301 gcgctccatg atccgcgcag cgtagaccgc cgcctgagcc cgacgtgaaa ccccgagttt
      361 cgcgaacagc gcggtgacgc ggtttttgat cgtcttttcc gcaaggtaca tgtgttcggc
      421 gatctcgcgg ttgctcttgc ccatcgcgat cagttcaaga atctcgcgtt cctggtcggt
      481 cagcgactca agcggctcgt tcgcttcacg gtcggctcgc acccgttcga gcgaccgtgc
      541 cgtgacatcg ggattcatcg tcgagcgccc ctgcgccgcc gctcgcacgg cggcgacgat
      601 ctcgttgcct ttcagctgtt tgagcgcgta gcccgacgcc cccgccagcg cagcttcgat
      661 gagcgattcc tcgtcgtcgt aggaggtgag gatgatgctg tgcatgcgag gtgcgctcga
      721 gcgcagttcg cggcagattt cgatgccgga cccgtccggg agtccgagat ccaacaccgc
      781 cacatccgcg ttgctcagtg ggatccgtgc gagggcatca gcgacggtcg ccgcctcgcc
      841 gaccacttcg aggtcggact cgcggttgaa caggtcgcgt aagccgcgcc ggacgatttc
      901 gtgatcgtcg acaagaaaga tccgcgatcc cacggcgcaa tgttgcacga ttcgcagtgt
      961 cccctgcgac tcgatgggta acggttgtca cccccgcgta ggcttgcacc atgcagggtt
     1021 cgctcggacg cgagaaccaa cggcgtctac tcgacgcgat cattcacatc ggttcggaac
     1081 ttgatctcgc acccgttttg cgtcgcatcg tcgaggtcgc aaccgaactc gtcgacgcga
     1141 actacggcgc gctcggcgtg ctcgacgaga caagaacgca tctcgccgag ttccacacgg
     1201 ttggcatgcc cgacgaccag gtcgaccgca tcggcgagcc cccgaagggc cacggcattc
     1261 tcggggtgct catcgtcgat ccccgtccat tgcggcttcc ggatctcacc aagcatccag
     1321 acagtttcgg tttcccgctc caccacccgc cgatgacgtc attgctcggc gtaccgatca
     1381 tcgtgcgcgg ggaggcgttc gggaacctct atctgtgcga caagcgagac ggcggtgtgt
     1441 tcagcagcga ggacgaggta ctcgtggtgg ccctcgcgtc cgctgctgcg atggcgatcg
     1501 acaacgcccg cctccattcg cgggtgtgcg acctggcgag aaatgaggat cgtgaacgca
     1561 tcgcccgcga tctgcacgac acggtcatcc agcgggtgtt cgccgtcggg ctgggacttc
     1621 agcaggcgat gctcatcgac gacctcgacg aggtccgcga gcgggtcgac accgccatcg
     1681 atgagctcga cgagacggcg cgagaggtcc ggtcggccat cttcgagctc aaggcggtcg
     1741 aacgcagcac gcggacgctt cgcgagttga ttgccagcac cgcggccgag gcggcccggg
     1801 tgctcgggtt cgagccgacc gtgaccctga ccggccccgt gaacgctggg gtgtccgaag
     1861 agcgcgcggt gcacgtgatt gcggtgattc gcgaggcgtt gtcgaacgtg gcgcgccatg
     1921 cggaggcgac ggcggtgacg atcacattgt cggtgggtga cggtcatctg tcgctgaccg
     1981 tggtcgatga cggggtcggg gttaccccgg gcgagatcgg tggtcagggg ctgtcgaaca
     2041 tggaaggacg cgccgcccgc ttcggcgggt cgatgctggt caccccggcg acgccgcagg
     2101 gatcggggac tcgggtgacc tggtcgattc cgctggagga ctgacccgct cgtcgggacc
     2161 gaccgggatc ggacaccgac cagaaggttc catcagtggc tggtcgatct ggcgatctgg
     2221 cgaatgcggg tctcgaggcg tcgcacgacc gtgtcgtagg cgaccggata cagcgacttc
     2281 gccagttcaa catccatgac gttgcccggg cgcaccccga tgcgcgacgc gaacgccgca
     2341 ccaggccgca acacgatggt cgtgccgcca tggcgttcgc gccaacgagc cattgctcgt
     2401 cgcgccgctt gttcgaaggc gcgtccgacg gggccgaaca ccgcccccgc gatcggcagg
     2461 ctcacgatca acaggtcggc gccagcggcc gaacccgccg aggccatggc gcggaccccg
     2521 ccgtcgacgt agcgtcggcc ggcgatctcg tggggcgaga acacaccggg caccgcggac
     2581 gatgcggcga tcagatcggg gagggggaac ctggaggcgt cgagcaggtg atggcgcatc
     2641 gtcggtaacg ccgtcaccga gatgcgcacg ttgtcgaggc gtgcctctcc gtagatctgc
     2701 gccgccaacc cgcgcaacaa tccgggggag cggtccggta ggtcgaggtc gccgaatccg
     2761 gcaaactcat cgaagctgag tccgagccgt gccgcgccgc atgcccatga tccggcgctg
     2821 gttccgatcg acggtgcggc gtcgaccggg agtccgagcg aacgcagtgc gtcgatcacc
     2881 gccacatgcc aggcgatccc catggccccg ccgcctccgt agaccaccga aatggtcggg
     2941 tcgcccggaa agcgacttcg actccgcggc ttcggacggg cgaaatcgct gaggtcgatg
     3001 acgcgctcgg acggtccgac gagagcggct gcgttcaaca tgtcgacatc gtgacaggtg
     3061 tcacgtcgcg tggcgaatgg gaagttcggc tcatccggtg aacggcccgt ccgaacagcg
     3121 tccggccgga gcacgaaccc gaggaactag cggatcttcc agccgatgcg gacactcaac
     3181 ggcggatgat cgctgagtgg ctcgcccgcc gcgtcgacga accgatcgct cacgaacctg
     3241 tgggattcga cacggagttc gacggtggcg ccacttctca ctgccacctt gtcgatctgg
     3301 tcccgttcgg cgcacgaggt ggcatcgcag gagtcggtga gcccggtgga cgcgagaaac
     3361 tcctcccaaa tccgggtatc ggcaccgtcg ctggcgtctt cgtggtcgct gtaggtgtgc
     3421 aggttggtgt cgccggcgac gatgaccgcg gcgtcggtgc tgtgttcggc gatgaacgtg
     3481 gcgagttgcc ggtagtcggc cacttggagg tcctggtcgg tgggggatcc gcccgcctcg
     3541 ccgtggaggt catagagatg gaccaggtcc cctgacggca aggtgacctc ggtcatcagg
     3601 aatcccttca tggcagcaca gtcggccgcg ccgccgtcgg aggtgtccag gccgccgaag
     3661 cagccgtccc atggaacccg acgagtattg gcgatcggca ggcgcgacaa cacgccgaga
     3721 ccgtcgccga tgaagggttc catccgggcg gcgaggtcga gaccgaccgc ctcagggcca
     3781 gggtgctgct cggttcggaa ttcgtgggtc gcatcggcgc ggaggcggtc gtggtagttc
     3841 acgaaatcga agttcgcagc gagaggaccc caccagtcga agtcctcctg agtggcgacg
     3901 atgtcgtagt cgttcaggag tggactgatc agcgggagat gctcggcggg atcgaccgac
     3961 gagatctccg cgggcaggcc ggccacgttg tagctgagca gcgtgaagct ccccgaggcg
     4021 ccctcctcgg ccacctcttc ggccccctct tcgatggtcg aggtcgtggt cgagggcgtc
     4081 gccgcggaag tgtcgcccga ggccgagtcg tcatcgccgg aacaaccgga ggcgaacagc
     4141 agtgccgtag ccacggcggt cgccagagct gcggttgtcc aacgtgtggt gcgggtcatg
     4201 gggaaccaac ctacccgtgg ggctcgatct cgtcgttgag ggccgccgtt gccgctcctg
     4261 taacttgttg gccatgtcag gacgtccgct cgtcgtcgtg ccggttcgag gaggggtcct
     4321 tcctgccggt gggctcgaat cgatcgccga ggcgcaggcc gcgggcgatg ctcgcatcgt
     4381 gctcatcgga gacggcctcg aagcggtcat cgccgatctc gcgtcgatcg ccgactcggt
     4441 cgaaacgctc gaagcgggcg ccttcgcagt aacggcctgg tcggcgctgc tcgccccgtt
     4501 ggcggccgag gccagcttcg tgttgctgcc cggttctccg gatgggcgcg atctggcggt
     4561 acgtctcgga gcccgcatcg aatgcgacgt cgtcaccggt gccctcgccg tctcggccga
     4621 cgcggtcacc gtcagccatc acgggggact ggtgcaaacg gcgctgcggc cgacgggtcg
     4681 attcatcgcc acgctgcaac cgaacgtccg cggggtcgac gcccacgggg gtcccgacac
     4741 cgaggagtcg gaggcgacgg tggtctcgct cgcggtgcag gtcgaaccct tggcgacggc
     4801 acgcgacgcc gctgtcgtgc gcgtcgaggc ccccgaggcg tccgctatgg atctggcgga
     4861 gtccgctcgg atcgtcggcg ccggagcggg cctgatggcc gacgagggtc aggccgatgc
     4921 gctgcgagcc cggttcgagc ggcttcgtgc cgtcggagca tccctcggcg cgtcgatggg
     4981 cgccacgcgg gtggtgaccg acgccgggtg ggtaggccat gaccgacaga tcggaaccac
     5041 cggagtcgtc gtcgaccccg agctctacgt ggcgttcggc gtcagtgggg cggtgcagca
     5101 caccgccggt ttgggccatc ccgaccacat catctcggtc aacctcgacc cgcactgtcc
     5161 gatgatggcc atggccgatc tggcggtggt cgcggacggt gccgcaaccc tcgacgccat
     5221 cgccgaactt ctgcacgttg gtgacggttc agctcaggag gtaaccaccg atggttgacg
     5281 tcgacgcgat cgtggtgggg gccggccctg ccggggcggc tgcggcaatt gcactggccc
     5341 gagccggcag gtcggtggtg ttgctggaac gcgggccgtt tccggggtcg aagaacatgt
     5401 acggcggggt gatctacgga cgcatcctcg acggcatcgt ggcgaactgg tacgacgagg
     5461 ctccgatcca acgctggatc acccggcgat cgacgatgat gatgaccggc catcaggcgc
     5521 tcaccatcga ctaccggacc agcacctggg gcgaggcccc ctacaacggt tgcacggcgt
     5581 accggcccga cttcgacaac tggctcgccg gccatgcgac cgacgccggt gcggaactga
     5641 tctgttcgac caccgccacc ggcctgctgc gcgaaggcgg gcgcgtcgtg ggggtgcgca
     5701 ccgatcgccc ggacggagac ctcaccgcca aggtggtgat cgcgtgtgac ggggtgaatt
     5761 cctttcttgc caaagaagcc gggatgtatc accacaccga ggcggagcac ttcacggtgg
     5821 gggtcaagga gaccatccat ctcggctccg acgtgatcga tcagcggttc gcacttcgcg
     5881 gccgcgaggg tgcggacttc gagatcgtcg gctgcacggg cggaatcccc ggtggcggat
     5941 ttctgtacac gaacctcgac accgtcgcga tcggtctcgt gctgcagctc ccgggtctgg
     6001 gtgcgcagga caagcgtccg gagcagttga tcgccgacct gaagtcgcac ccgtcgatcg
     6061 ccccgctcgt cgagggcggc gaggtcaagg aatacagcgc acacctgatt cctgagggtg
     6121 gctacaacat gatgccggac ctggtcaccg acggaatgct cgtcgccggc gacgcggcgg
     6181 cgatgtgcct ggccgccggc atctggctgg agggcgtcaa cttcgcgatc gcctcggggt
     6241 tcgctgccgg acagagcgcg gtcgacgcgc ttcgtcgcgg cgatacctca gcagacggtt
     6301 tgtcgcgata ccgccaccgg cttgagacgt cgttcgtgtt ggcggatcac ctcaagctcc
     6361 gcgcggtccc cgacctgatg atgaacgaac gggtgcagca gcggtacccg caactggcgt
     6421 gcaacgtcgc cgagcggatc ttcacggtcg agaaccctcg gccgaaaccg ggcttcaaca
     6481 ccatcgtgag atccgagctc aaggcgtcgg ggctgaagat gcgcgaggtc ctcaaggacg
     6541 ccgtgaccgg gttgaggagc ttcggatgaa cgccagcgag gacaactacc tcgacatcag
     6601 ttttgagcat cgcatgggac tcgttgagtt ccgggtcgcc gagacggccc acatcaccgt
     6661 cgacggcgac gcgtgtcgcg gctgcacgac gatgcaatgc gtgacggcct gcccggcgaa
     6721 tctgttcatc cccacctccg acggcggcat cttgttcaat tacgagcagt gcttcgagtg
     6781 cggcacctgc tacatggtgt gcaacaccga gggcgcgatc tcgtggagtt acccgaccgg
     6841 cggccacggc gtcgtgttct cgaagtccta ggggttcggc ggtgaacgac acggcattga
     6901 atgggttgaa tcccctcgga cgaacggcaa ccagcggggt accggccgct cttggcgtgc
     6961 cgaggagcgc gatcgtcgtc tgcgtgaact gggtcgacct tcacccggag atcgacctga
     7021 tcgacggttc gatctcgacc gaccgtcgcc gttacgggtt ctccgaggcc gaccgggcgg
     7081 cgcttgaggt cggcctcggc attgccgatc gcgaccgcgg cgacggctgc gatgtggagg
     7141 ttgtcgtgat gtcggtcgcc ccgagcgcgg cggatgactc actgatcgaa ctggcggcct
     7201 cgggcgccga ccggatcgtg cgcatcgacg gtgaccccga cggtgatccg gcgttgtcgg
     7261 ccgccgccgt cggccgagcg ctcgcccacg gcatcgcggc gatcggcgat gtctcgctgg
     7321 tggtgtgcgg cgacctcggg ttcgcttggg gcagcggcgc ggtgccggga tacatcgcac
     7381 atcatctcga ggtgcctcag gcgctcggtg cgcttgaggt gtcagcggcc gtggaggccg
     7441 tgggggtcgc cgaggtggga cccggcgaac tctcggtgct tcgacgcctc gacggcggtc
     7501 gtcgcgagct gttgcgggtg ccccgacctg cggtggtgtc ggtcgaaggt gccgcggctc
     7561 gactgcggcg ggcaccgctg cgagcggtca tggagttgcg gcgacgaggc gtccgcgtgg
     7621 attcagttgc tttgcccatg ccgtcagggt tgctggccga acacgtcggg ccgattcgtc
     7681 cgcgggcccg cgtcctggcg ccgccggggg ccacccgtgc gctggaccga atcgcggagc
     7741 tcaccggagc gttcgtcgat cgcaacccgc cccgcaccgt cgaggccgaa ccagccgaag
     7801 cggcggcgct gatcgttgaa cagctgcgga cgtggggata cctcaccgac gatccgattc
     7861 cgtgaccgca cgacagctcg gcgacgcgac ctggccggag atcgcacagc gtttgtcaac
     7921 gctggtgatc ccgctcggct cgaccgaaca gcacgggccg catctgccgc tcgataccga
     7981 cacgcgtatt gctgttgcgt gtgcgacgcg tctggtcgag gcgcgccccg atctcgtgct
     8041 gggcccggcg atggccatcg gcgcctccgg tgagcatgcg ggattcgccg gcaccttgtc
     8101 gatgggggcc gaggtgttcg aagccgccat cgtcgagatc ggccggtcgg ccgacgcgtt
     8161 cgatggggtg atttgggtga acgcccacgg cgggaactca gtcgccttgc gcagcgccca
     8221 cgcgacgttg gtgagtgaaa gccggaggtc gctggtgctg cgttgcgcgg tgcctggcgg
     8281 cgacagccac gcgggacgta ccgagacctc gatgatgctg tcgatcgccc cgcaggtggt
     8341 tcgaagcgac ctcgccgcgc cggggcgcag cgaaccctgg gcggagttgt ccgaggcgat
     8401 cctggccggg ggagttgtgg ccgtgtccga caacggcgtg ctcggcgacc ccgcaggggc
     8461 gacggccgag gaggggaagc gatttctcga cgggatccac gcgcaatttc tcgacgcggt
     8521 ggcacgctgg atcgatggcg actcctcctg aaccgcagaa cccgaccggc gccggacccg
     8581 atggtggggc catcgtcggg gtggcgctcg tcaccggcgc ggcgcggggc atcggcgcag
     8641 cgaccgtgcg tcgactgctc tcgcaaggct ggggtgtggt ggcgaccgat atctgcggca
     8701 acatcgacgg cctcgggtac ccgatgggga cgctgaagga gttgagggcg gtcgtcgacg
     8761 gtagcggtgg tcgtggtgtc gccgttgtcg ctgatgtgcg cgatcggcgt gcgctcgacg
     8821 cagcggtcga gatcgcgctc gaccgcttcg gccgcctcga tgccgccatc gcctgcgccg
     8881 gggtgatgct gggggcgacc cacgggtggg agatgacccc ccgggccttc gacctgtgcg
     8941 tcgacatcaa cctcggtgga gtccagcgat gcgcctcggc ggccctgccg gcgatcatcg
     9001 aatcggcgac cccgggggcc ggaaggtttg tcgccgtcgc gtcagcagcg gcgagttcgg
     9061 gacgtccgca gatcgcggcc tacagcgccg cgaaggcggg ggtgatcggg ctcgtacgtt
     9121 cgctcgccgc cgaactcgcg ccgctcgggg tgaccgccaa ttgtgtgtct ccggggtcga
     9181 ccgacacgac gattctggcc gcaagcgcag ccttgtacgg tctcgccgat cccgccgaat
     9241 tcgtggccca tcagcccgtc ggtcgtctcc tcgaccccga cgaggtcgca gccggcatcg
     9301 cctggttgtg ttcgccgcag cgtgggggcg tcaccggcat cgtgttgccg atcgacggcg
     9361 gcatgtccct gtgacgactc gccggtaccg actcgctggg tcggttcgat cggtcgccgg
     9421 tgggcgtggc ctcctcggcg gagacccgat tcggttgatg cgcctgtccg tagcggggtc
     9481 gcgtctggcg cgctcctggg gcgatgacgg catcgtggtc ggcagcgggt cggaggcggc
     9541 cgatgcgttg gcggaacggc ttgtcgacgc agggatggcc gtgccggtcc ccgcactgct
     9601 gactccggcg gagctcgccg ccgcgctgga ggacctgacg gtgatcgtcc ccgtcttcga
     9661 gcgcgtcgac gcgttggatc gctgcctgaa ggccttgcgc aactcggggg tccgcaacat
     9721 cgtcgtgtgc gacgacgcgt ccccgaacgg ggacgaaatc cgcgaggtct gcacccgcca
     9781 cgacgcccgc gtggtgcgac gcggcgccaa cggcggcccg gccgcggccc ggatgaccgg
     9841 ttgggtgtcg ttggaacgac ccgatgtcgg tttcatcgcc tttgtcgact ccgacgtcga
     9901 ggtgtctcca tcgtggctac atccgttgct cggactgttt ggcgatgagc gcgtcgcgtt
     9961 ggccgcgccg cgggtggccc atctcgagac cgacggcggt gtgctcgacc gctatgagga
    10021 agcgaattca ccgctcgacc tcggcggtcg gcaggcagcg atcagcgccg gaacccgggt
    10081 gtcgtatgtc ccggccgcag cgcttgtcgt ttccggcgca gccttttccc aggtcgatgg
    10141 tttcgatcgg agtctcagcg tcggggagga cgtcgatctg tgttggcgac tcgtcgattc
    10201 cgggtggcgg tgtcgttacg agccggcatc ggtggtggag catcgcggcc ggtcgtcgtt
    10261 gggggcgatg ctggcgcggc gcatcgacta cgggcgctcc gcagcacaac tcgatcgccg
    10321 acatcgcgga gcgctgccgc cgctgcgagc caacgtctgg tcgttggcgg cggtcgccgc
    10381 gctcggcgcc cgtcgcccgg gcatcgctgt tgcgttgacg gcgtgggccg tgcggtcgat
    10441 gtcgaaacgg ctcgacgaca tcgaaccccg gatcgtggcg cgtctggtgc tgtcgggcga
    10501 tgccggagcc ggtcgtcaac tggcccgagc cctggtgcgg ccatggcttc cggtgacgtt
    10561 ggtcgccggg gcggcgtcgc ggcggattcg gcgtgttgcg atgatcgcgg tggcggtcga
    10621 cctgttgtgg cgttgggcgg aacgctcgcc gcgtttggat ccggtgcggt tcggcgcgtt
    10681 atgcctgctc gacgatgctg catacacgct gggggtgtgg atcgggtgcg tgcgggaggg
    10741 tcgcatcggt gtgctgttgc cagagatctc cgtggcagcg ccgaactcgg ctggcaaaaa
    10801 gtagaatctg ttctagtatt tcgtttcgcc cgcacggggg tgtgggctct acgaaatgaa
    10861 ggggacagtc catggggaat gccgcagggt cttcggacct gctccgcgcc gacctggtgc
    10921 tcgaatatcc gttcaaccgg accaccggcc ccgtcatcgg agcgttctac accggcctgc
    10981 gcgagcaggt cgtcgtgggt atccgtgacg cagacggccg agtcgtgtgt cctccggttg
    11041 aatacgaccc gacgaccggc gcgccgctaa ccgaactcgt gaaggtgggc gacaccggca
    11101 cgatcacctc atggacctgg gtcgacaacc ctcgcgaagt cgaggctctc gatcgaccct
    11161 acgctctggc cctgattcaa ctcgatggcg cggacacagc gatgctgcag gtcgtcgacg
    11221 cacccggacc agacgcactc tcgacgggtg cccgcgtgaa gatccgctgg gcggccgagc
    11281 gtagcggcgc gatcaccgac atcgaagcgt tcgaactggt ggagggctga gatggctgct
    11341 gaatcaaaca ttgccgagtt gccggccgga ttgtgggccc agcctgcgat cgacacctct
    11401 gctgtgggcg aggttgtgga aaacgaccct gttcaacgcg ttcgcctccc cgcctcgatt
    11461 cgctacgact tcgttcccgg cgtggcgacg acccgtttcc tcaaggggct gaaggaaaag
    11521 aagatcctcg gagaacgcga ggccgactcc gacgatgtct acgtgccgcc ccgcggcatg
    11581 tcgcccacct cgggcaagcc gaccacggtg caggtcgagt tgccacacaa aggatccatc
    11641 gcgtcgtatt gcgtcgtgca catcggcttc ggtgtcaacg ccccgccgac cccgttcgta
    11701 tcggccctcg tgttgctcga cggcgcctgc atctcgatct acggacccgt gcaggagatc
    11761 gactacgaca aggtgcgcat cggcatgcgc gtcgagcccg tttgggtcga cgacgaccaa
    11821 ctcgacacga gcttcgaaag catcaagtac tggcgcccga tcgacgagcc cgatgtgccc
    11881 gccgagagcc tgaagggaca catgtgatgg ccgccgaatt cgccgccccc tcccgcgacg
    11941 tcgccatcgt gagcgtcgcc caatccaagc acgaacgcgc catcgttgaa cgcaacgagg
    12001 tcgagctcat ccagccggtc atccagcagg cgatggaaca gatcggtgcc cacatcagcg
    12061 agatcgactt cgtctgttcg ggaagctccg actatctcgc cgggcaggcg ttcagtttcg
    12121 tgatgaccct cgacgccgtt ggcgcctggc ctccgaagtc ggagagccac gtcgagatgg
    12181 acggtgcgtg ggccctctat gaggcctggt tgaagatcca ggcaggccat gccgacaccg
    12241 ccttggtcta tggctacggc aagagttcgc cgggcgactt gccttcggtg ctcagtcgca
    12301 aactcgaccc gtac
//
LOCUS       DAOOMA010000013        12281 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_5713_length_12281_cov_4.049894, whole genome shotgun sequence.
ACCESSION   DAOOMA010000013 DAOOMA010000000
VERSION     DAOOMA010000013.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 12281)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12281)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12281
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5713_length_12281_cov_4.049894"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1590)
                     /locus_tag="PLP95_00835"
     CDS             complement(<1..1590)
                     /locus_tag="PLP95_00835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB44378.1"
                     /translation="MKVKRWIAVGLTAAVATVGLEAIAPVGAGATAVSINCQGITGDN
                     ASSLGDSKATLGLLSSISPGGGSGLSFPAEITTNAPAKVQPNTGPFNADFNLTMTMPA
                     SLMGPARDLLGMSAVTVTDATYAIQASGAASTLISKTVPSEVISLTSNPVVIQQSMSG
                     TITPTGPGAIIYSVSASTRLTIAINQSVAGVQINALTVTCTGGNDIGMTAVQIPGSPN
                     VAQPLNVAGYTAAVTGLGLLGSNITPDNNNAIHPDTLAVTAQAPNGGYTAVGGGAAFF
                     LAPQEPGYYVANYNVCAGSLPVAAIPGANATQTLTWPESYADKGLNAHPLSMRLGFKG
                     QTTSPISLAQLNGQPSPSNEKMLDFFHRFFGKFVAPSPTAVQTALEKLSTIGKGNVKV
                     TRHEGGGYDIEFVGSLADAPQPAIEIAEWNTWLPSDGLASLMALMPGASGGEAGGAAP
                     KTADQLNNELMSGAIKFDEWLEGQGELLKANIIAGATTPEMIANITAMFPKSPEVAVV
                     NAGKTTIPASETGPLCSPFQIG"
     gene            complement(1648..3192)
                     /gene="lnt"
                     /locus_tag="PLP95_00840"
     CDS             complement(1648..3192)
                     /gene="lnt"
                     /locus_tag="PLP95_00840"
                     /inference="COORDINATES: protein motif:HMM:TIGR00546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006497 - protein lipidation [Evidence
                     IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HPB44379.1"
                     /translation="MLSAVNVTPPSSRAARIALSLGAGLAMCAGLPPWGWWPLTIVGV
                     AVWALLLHDQPARARFWIGAGVGLALYLPSTMWMVKMTPLGWPIGVSIWFPLVFGLVS
                     ASCPPSGVALALPGAVVLAEWVRWHAPFGGVPLSMFAYTQARGPLLPVARIGGTLAVS
                     AAVAMAGVALAGLVRRHTYRNAAITAAALILVTLGGVVAPRGTATSQIRVAAVQGGGE
                     QETEHFATDYDEVLQRHIDTALTLSGHADLVVLPENIVNVNGRWETSSERARLVELNR
                     KLQTTLIVGVVEDRDSADHFWNFATALNPNGNDEGRYDKVRRVPFGEYVPLRGLIARF
                     ASGQLPGRDAVPGDLPAVLHTDLATIGVVISWETFFPRRVRDALHHGAEILTNPTNGS
                     SYWLSQVQTQQVASSTLRAVESGRWLVQAAPTGMSAIIDPNGNVVARSGIGEATVLEA
                     TAELRTGTTLAMRWNEVPVLVLSAIAVALAWRRHATRKNPESSGEDRFQVPSGNGEDN
                     PLPSNA"
     gene            complement(3209..4561)
                     /gene="hemG"
                     /locus_tag="PLP95_00845"
     CDS             complement(3209..4561)
                     /gene="hemG"
                     /locus_tag="PLP95_00845"
                     /EC_number="1.3.3.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004729 - oxygen-dependent
                     protoporphyrinogen oxidase activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protoporphyrinogen oxidase"
                     /protein_id="HPB44380.1"
                     /translation="MTDIAVVGGGVSGLAAAWELSAHPDVHVTVVESSNRLGGKIRTE
                     PFVGREVDLGPDAFLRRVPDALALCAEIGLDDLIAPAHGTARVYLDGALVPIPTGLVL
                     GAPAHFDDLDRSTILSAAGLERAHGEAGLAGLPIDEDAAIGGLIRARYGDEVAERLVG
                     PLLGGIAAGDLDEMSLDACAPQLAAAARQGPSMTAALRSALESTTSTAGVEPVFAAPR
                     GGMEVLISTLVERLRERGVEFRLNTPVDRLPDADGVVLSADAALHAKLLRDRVPEAAQ
                     LLSSIEFASVVFTAIAFSADDLPTDLDASGFLVPRGAGLTVTAASWSSTKWAHLAGDP
                     AILRVSMGHRHDHRSIDASDDEVIATIRRDLNTTMGITAAPVDVRIARFRKGFPQYRV
                     GHLELTERVEQLLATGTPRVALCGMAHRGVGIPACVREARRAASGLIDRVQNLGHDSR
                     "
     gene            complement(4558..5472)
                     /gene="hemH"
                     /locus_tag="PLP95_00850"
     CDS             complement(4558..5472)
                     /gene="hemH"
                     /locus_tag="PLP95_00850"
                     /inference="COORDINATES: protein motif:HMM:TIGR00109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004325 - ferrochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HPB44381.1"
                     /translation="MNQRIGVLVMAYGTPGSTEDIEAYYTHIRHGRPPEPTQLADLTA
                     RYDALGGTSPLAARTEAQRAAIADALDATDPGRFVVALGQKHTSPFIEDGVATLAEYD
                     VDRIIGVVLAPHYSRASVGEYHARAAETAGERDIEYVGIDDWYALDAHVSFHASALRV
                     VLDEMPQRTKVVFSAHSLPERVLVDDPYADQLFAGAELIATRAGLARWAGWGIAWQSA
                     GRTPEPWRGPDLLTVIGDLADTGRAEGIVVVPHGFTSDHLEVLYDIDIEASRHAARRG
                     LELRRPSVVNADPAVMGALAAQIRALAS"
     gene            complement(5469..6518)
                     /gene="hemE"
                     /locus_tag="PLP95_00855"
     CDS             complement(5469..6518)
                     /gene="hemE"
                     /locus_tag="PLP95_00855"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HPB44382.1"
                     /translation="MTSPASPSDPFLAAARSEPHDRIPVWFMRQAGRSLPEYRAIRGS
                     GSILHAIAQPDLATEITLQPVRRYGTDAAILYSDIVTPVHAIGFGVDIVPGVGPVVER
                     PFERAADLDRLRPLEADVDTPYVIETVKNLVAELPCPLIAFAGAPFTVASYLIEGRPS
                     RTYTKVKSLMYRDEALWHRLMDRLADLAIASVTSQVTNGASAAQIFDSWAGALSPDQY
                     RRFVLPSSQKVFDGIRSLGVPTIHFGVHTGELLGLMAETGTDVVGVDWRTPLDAARRR
                     VPERCALQGNLDPAVVIAGWEATRAEAESILVRNAGHPGHIFNLGHGVLPETDPGVLE
                     QLVAWLHEQPAGGRP"
     gene            6655..8718
                     /locus_tag="PLP95_00860"
     CDS             6655..8718
                     /locus_tag="PLP95_00860"
                     /inference="COORDINATES: protein motif:HMM:NF024642.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HPB44383.1"
                     /translation="MPTKSELEAEQAYIDRAYDCLERTRERARSVGSDPEADRGGTFQ
                     ARYERDVLIERAQHRLAQTDLGDQSLCFGRIDLESGERYYIGRIAVSDERAEPVVVDW
                     RAPVSEPFYRATGNDPMGLSRRRHFASSGRVLRGIEDEFFGEARDRLGDGRVNGEGAL
                     ISALETARSGKLGDIVATIQGEQDEVIRAPLSGVLVVQGGPGTGKTVVALHRAAYLLY
                     THRFPLEGQGVLVLGPNRLFLSYIEQVLPSLGEAGVEIAVIADLVPNVRATGFDPPEA
                     ARLKGDLRMCNVIAKAVRDRQRPLRETAVIPLGVERLRITPEDTRELISQTRRRSRTH
                     NGGRKAFLEGFYALVASKMREPLDPGVVRDRIRSLPETREIVERVWPTLSPAQLLNDL
                     FSSKGLTRLAANKWLSDTEQLLLLRSRADDADDIVWSVHDVPLLDEALELLGPRPKFR
                     ETETVRTYGHIVVDEAQDLSPMELRVIDRRSLNGSMTIVGDIAQATSASAHDSWDSVL
                     ANLPAKRSPRFTELAIGYRVPAPAMELASRVLRLAAPGLRPPRSIREVGDEPIIEATT
                     VDRFAEALATAVRREVAEIGAGSVAVICATSWIARVEDALTSGGVDFGRAHRGRFDHQ
                     VTVAPITLVKGLELDATIVIEPEAVLDQEFRGAQALYVGLTRATKRLSLLHVGPLPDV
                     LVEPL"
     gene            complement(8759..10297)
                     /locus_tag="PLP95_00865"
     CDS             complement(8759..10297)
                     /locus_tag="PLP95_00865"
                     /inference="COORDINATES: protein motif:HMM:NF012363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylesterase family protein"
                     /protein_id="HPB44384.1"
                     /translation="MTTQQDHSTLTVDTAAGTVRGQWLDGVARFAGIPYAEAPVGALR
                     FRAPQPRNPWQGVFDATEFGPIAPQNPSFMDLLFGLEPEPQSEDCLRLNIYTPDPGLS
                     GDEALPVMFWIHGGAFEMGSGSTSIYDGTAFAKQGAVFVSINYRLGPLGFLELSGLDD
                     EFLGSGNAGLLDQIEALRWVQTNIARFGGDPDNVTVFGESAGSMSTSLLMSSSLTDGL
                     FHRAICQSGGVNAARDLEFARTEGAEVMRLGGWSSVTEAQNAPLADLLAVHATIMAQR
                     WGNPNTTLAESGELLAAMAFRPVRDGHAVPTDPLAAIAQGAAAGVPLVIGHNRDEWRL
                     FALLMPGAETEEQLVERATLLGADAATLIEAYRIDHPEASPAELECAIITDIVFRVPT
                     AELAAAHSSHAPVWQYQFDWESPMLGAAHAVELPFVFSQLHDTRLAPLFGPEPPQQVA
                     DAMSNAWATFARDGIPSIASLDSWPHLPGNGSRPVVRFDVETTLDDNPLAASLAYWLR
                     GANG"
     gene            complement(10294..10986)
                     /locus_tag="PLP95_00870"
     CDS             complement(10294..10986)
                     /locus_tag="PLP95_00870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012223301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HPB44385.1"
                     /translation="MTNARRPSRGNKAGEFHPAFEEYCEAIFELQEDDLDVIQARIAE
                     RLEISRPAVSEMIRRMANEGLIVIGDSITLTEEGQRLAEAVVRRHRLAERMLTDVLGL
                     SWALAHEEAGKWEHVISPLVEERLAELLGNPSTCPHGNPIPGSNYRPPETIALANLPV
                     GTTFTVRRIPEELEFADGMLDFLEDHSLQPGKQGTVVSSGLRGEQVVEIAGATVALGA
                     FASERILVSIGP"
     gene            10985..>12281
                     /locus_tag="PLP95_00875"
     CDS             10985..>12281
                     /locus_tag="PLP95_00875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020379259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HPB44386.1"
                     /translation="MSACYLVLGMPNNGIRSSGARRSLSVPGGIVPAMGLAEFSEPTT
                     AWFTTTFGQPTEAQARAWPEIAAGRNVLLCAPTGSGKTLAAFLAAIDRLGSSPVSQKG
                     SGKSIERTTVLYLSPIRALAVDVEKNLRAPIRGINLAAQRLGVEFREPTVGVRTGDTP
                     ASLRRALVNHPPDILITTPESLFLMLTSAARESLRSVDTVIIDEIHAVAATKRGSHLA
                     LSLERLHDLADAAPQRIGLSATQRPLSEIAHFLGGNEVDSDGELRGQRAVTIIDEGGR
                     KPLELEVVVPVDDMGGVGESDLTPRSIWPSVNTAVLDLIEAHRSTIVFVNARRLAERL
                     ATRLNELEEERDRTGDVALAAERGGENFGVSPSASGHELVRAHHGSLSREQRLVVEDE
                     LKAGTLRGLVATSSLELGIDMGAVDLVVQVASPGSVASGL"
BASE COUNT         1975 a         3915 c         4323 g         2068 t
ORIGIN      
        1 gccgatctgg aacgggctgc acagcggccc ggtttcggag gcgggaatcg tcgtcttgcc
       61 ggcgttgacc acggcaactt cggggctctt ggggaacatg gcggtgatgt tcgcaatcat
      121 ctcgggggtt gttgcgcccg cgatgatgtt ggccttcagc agttcgccct ggccctcgag
      181 ccactcgtcg aacttgatcg cgccgctcat caactcgttg ttgagttgat cggccgtctt
      241 cggggccgcg ccgcccgcct cgccgccgct cgcaccgggc atcagcgcca tgagcgatgc
      301 cagaccgtcg gagggaagcc aggtgttcca ctcggcgatc tcgatcgccg gttgcggagc
      361 atcggccaag gagccgacga actcgatgtc gtagccgccg ccttcgtgcc gggtgacttt
      421 gacgttcccc ttaccgatcg tcgacagctt ctccagcgcg gtctgcacgg cggttggtga
      481 cggagcgacg aacttgccga agaagcggtg gaagaagtcg agcatctttt cgttgctcgg
      541 ggacggttga ccgttgagct gagcgagcga gatggggctc gtcgtctggc ccttgaagcc
      601 cagcctcatc gacagcggat gggcgttgag ccccttgtcg gcatagctct cgggccaggt
      661 gagggtctgg gtcgcgttgg cgcccggtat ggccgcgacg gggagtgatc cggcgcagac
      721 gttgtagttg gcgacgtagt agcccggctc ctgtggggcg aggaagaacg ctgcaccacc
      781 accgacagcg gtgtatccac cattcggcgc ttgggcggtc accgcgaggg tgtccgggtg
      841 aatcgcgttg ttgttgtccg gggtgatgtt cgagccgaga agcccaaggc ccgtcaccgc
      901 ggcggtgtag ccagcgacgt tgagcggctg ggcgacgttg ggcgacccgg ggatctgtac
      961 cgcggtcatg ccgatgtcgt tgccgccggt gcaggtcacg gtgagggcgt tgatctgcac
     1021 tccggcgacc gactggttga tggcgatggt caggcgagtc gacgcggaga cgctgtagat
     1081 gatcgctccc ggacccgtgg gagtgatggt tcccgacatg gactgctgga tgaccacagg
     1141 gttggacgtc agcgagatga cctcagacgg cacggtcttg gagatcaacg tgctcgcagc
     1201 gccgctcgcc tggatcgcgt aggtggcgtc ggtgacggtg accgcggaca taccgagaag
     1261 gtcgcgggcg gggcccatca acgatgccgg catcgtcatc gtcaagttga agtcggcgtt
     1321 gaaaggacct gtgttgggct gaacctttgc cggggcgttg gtcgtgatct cggccgggaa
     1381 cgagagcccg gagccgccgc cggggctgat cgatgagagg agtccgagcg tcgctttcga
     1441 gtcgcccaac gacgaggcgt tatcgccagt gatcccctga cagttgatgc tgaccgctgt
     1501 ggcaccagca ccaactggtg cgatggcctc aaggcccacg gttgcgacgg cggctgtgag
     1561 gccgaccgca atccaacgtt tcaccttcat agctcccccg gaaagtccgt tcggtgttcg
     1621 cccgtagaca tgcggacgcg tgtctaccta ggcgttggac ggtagcgggt tgtcctcacc
     1681 attaccagag ggaacctgaa aacgatcttc ccccgaggat tcggggtttt tccgcgtcgc
     1741 gtgacgcctc cacgcgagtg ccacggcgat cgcggagagc acgaggaccg gtacttcgtt
     1801 ccagcgcatc gcgagcgtgg tgccagttcg gagttccgca gtcgcctcca ggacggttgc
     1861 ctcgccgatc ccggaacggg caacgacgtt gccgtttggg tcgatgatgg cgctcatccc
     1921 ggtgggagcg gcctgaacca gccacctgcc gctttcgacc gcgcgcaggg tcgaggaggc
     1981 gacctgttgt gtctgcacct gggagagcca gtagctcgaa ccgttggtcg ggttggtgag
     2041 aatctccgct ccgtgatgga gagcgtcgcg gacgcgtcgg ggaaagaacg tctcccacga
     2101 gatgacgacg ccgatcgtgg cgaggtcggt gtgcaggact gccggcaggt cgccagggac
     2161 ggcatcgcgt cccggaagtt ggcccgacgc gaatcgtgcg atgagtccgc gcagcgggac
     2221 gtactcaccg aacggcacgc ggcgcacctt gtcgtatcga ccttcgtcgt tgccgttcgg
     2281 attgagcgcg gtcgcgaagt tccagaagtg atcggcgctg tctcgatcct cgacgacccc
     2341 cacgatgagg gtggtttgga gcttgcggtt gagttcgacg agacgtgccc gttccgatga
     2401 cgtctcccac cgcccattga cgttcacgat gttctccggc aggacgacga ggtccgcatg
     2461 gccgctcagg gtgagggccg tgtcgatgtg tcgctgcagg acctcgtcgt agtcggtcgc
     2521 gaagtgctcg gtctcctgtt ccccgccacc ttggaccgca gcgacgcgaa tctggctggt
     2581 ggcggtgccg cgcggggcga cgaccccacc gagggtcacg aggatgagcg cggcagcggt
     2641 gatcgccgcg tttcgatagg tgtgacgccg aaccagcccg gcgagcgcca ctccggccat
     2701 ggcgaccgcg gcggacaccg cgagggtgcc gccgatgcgg gcaaccggca gcagtgggcc
     2761 tcgggcctgg gtgtacgcga acatcgacag cggcacacca ccgaacggcg cgtgccagcg
     2821 gacccactcg gcgagcacca cggcgccggg gagtgcgagc gcgacacccg acggcggaca
     2881 agacgcggaa acgagaccga acacgagtgg gaaccagatc gacacgccga tcggccaccc
     2941 gagcggtgtc atcttcacca tccacatcgt ggacggcaga tacagcgcga ggccgactcc
     3001 ggcgccgatc cagaaccgcg ctcgtgccgg ctggtcgtgc aacaggagcg cccaaaccgc
     3061 cacaccgacg atcgtgaggg gccaccatcc ccacggcgga agcccggcgc acatcgccaa
     3121 tccggccccg agcgagagcg cgattcgggc agcccgagag ctcggcgggg tcacgttcac
     3181 ggcgctcagc atggcagcaa tcgggtgttc aacgcgagtc gtgccccagg ttctgaacgc
     3241 gatcgatcag cccactcgct gctcgacgcg cctcgcgaac acacgccgga atccccacac
     3301 cgcgatgggc catgccgcac aacgcgaccc gcggcgtgcc tgttgccagg agttgttcga
     3361 cccgctcggt caactcaagg tggccgaccc ggtactgagg aaatcccttc cggaaccgtg
     3421 cgattcgaac gtcgaccggc gccgcggtga tccccatggt ggtgttcagg tcgcggcgga
     3481 tggtggcgat gacctcatcg tcggaggcat cgatcgagcg atgatcgtgg cggtgtccca
     3541 tcgagacgcg aaggatcgcc ggatcgccgg caaggtgcgc ccacttggtg gacgaccacg
     3601 acgcggcggt gacggtgagc ccggcacctc gggggacgag gaaccccgag gcatcgaggt
     3661 cggtggggag atcgtcggcg gagaatgcga tggccgtgaa cacaaccgac gcgaattcga
     3721 tcgacgacag cagctgggct gcctcgggaa cccgatcgcg cagcaacttc gcatgcagcg
     3781 ccgcatcagc gctgagcacc acgccgtcgg catcggggag ccggtcgacc ggggtattga
     3841 ggcgaaactc gaccccccgt tcgcggaggc gctcgaccag tgtggagatg agcacctcca
     3901 tcccgcctcg aggcgcggcg aagaccggct cgacacccgc ggtcgacgtc gttgattcga
     3961 gcgcggaccg cagcgcggcg gtcatcgacg gcccttgccg tgcggccgca gccagctgcg
     4021 gagcacacgc gtcgaggctc atctcgtcga ggtcgccagc ggcgattcct ccgagtagcg
     4081 ggccgacgag tcgctcggcc acctcgtcgc cgtagcgagc ccggatgaga cctccgatgg
     4141 cggcgtcctc gtcgatcggc aatcccgcca accccgcttc gccgtgagcg cgttcgagtc
     4201 cggccgcgct cagaatcgtg gaacggtcga gatcgtcgaa gtgggcgggt gcgccgagca
     4261 cgaggccggt ggggatcggg accagcgcgc cgtcgaggta gacgcgagct gtgccgtggg
     4321 ccggagcgat gagatcgtcg aggccgattt cggcgcacag ggccagcgcg tcggggacgc
     4381 gccgcagaaa cgcatcgggt cccaggtcga cctcgcggcc gacgaacggt tcggtgcgga
     4441 tcttgccgcc caagcggttc gacgattcca cgacggtgac gtgaacgtcg gggtgtgcgg
     4501 ataactccca tgccgcggcg agtccggaca ccccgccccc gacgactgcg atgtcggtca
     4561 cgaggcgagc gcccggatct gtgccgcgag cgcgcccatc acggccgggt cggcgttgac
     4621 caccgaagga cggcgcaact cgagtccccg gcgggccgca tggcgcgacg cttcgatgtc
     4681 gatgtcgtag agcacctcaa ggtgatcgga ggtgaagccg tgaggcacca ccacgatgcc
     4741 ttcggctcgg ccggtgtcgg ccaggtcgcc gatgaccgtc aacaggtccg gtccccgcca
     4801 cggttcgggt gttcgtccgg cgctttgcca ggcgatcccc catcctgccc aacgggccag
     4861 cccggcacgg gtcgcgatga gctcggcgcc ggcgaagagt tggtcggcgt aggggtcgtc
     4921 caccagcacg cgttcgggca gggagtgagc gctgaacacg actttggtgc gttgaggcat
     4981 ctcgtcgagc accacccgca gcgcgctcgc atggaacgag acgtgggcgt cgagcgcgta
     5041 ccagtcgtcg attcccacgt actcgatgtc gcgctcgccg gcggtctctg cggcgcgggc
     5101 gtggtactcg ccgaccgagg cgcgtgaata gtggggggcc aagacgaccc cgatgatgcg
     5161 atcgacgtcg tactccgcga gggtggcgac gccgtcctcg atgaacgggg acgtgtgctt
     5221 ctggccgagt gcgacgacga agcggccagg gtcggtggcg tcgagcgcgt cggcgatggc
     5281 ggctcgttgc gcctcggttc gtgccgcgag cggggaggtg ccgccaaggg cgtcgtatcg
     5341 tgccgtcagg tcggcgagtt gtgtcggctc gggcggacga ccatggcgaa tgtgggtgta
     5401 gtaggcctcg atgtcctcgg tggatcccgg cgtgccgtag gccatcacca acacgccgat
     5461 ccgctggttc atggtcggcc tccggcgggc tgctcgtgca gccatgcgac cagttgttcc
     5521 aacacaccgg gatcggtttc gggaagaact ccgtgtccca ggttgaagat gtggcccgga
     5581 tgtccggcgt ttcgaacgag gatcgattcg gcctcggccc tggttgcctc ccagccggcg
     5641 atgacgacgg cgggatcgag gttgccctgc agcgcgcacc gctccgggac ccggcggcgt
     5701 gctgcgtcga gcggggtgcg ccagtcgacc ccgaccacgt cggttccggt ctcggccatg
     5761 agtccgagca actcgccggt gtgcacgccg aagtggatcg tcggcacgcc caacgagcgg
     5821 attccgtcga acaccttttg cgacgacggc aggacgaagc gtcggtactg gtcgggagag
     5881 agcgctcccg cccatgaatc gaagatctgg gcggcgctcg cgccgttggt cacctggctc
     5941 gtgaccgagg cgatggccag gtcggccagc cgatccatca gccggtgcca cagcgcctca
     6001 tcgcggtaca tgagcgactt caccttcgtg taggtgcgcg acggacgccc ttcgatcagg
     6061 tagctcgcga cggtgaacgg agcacccgcg aacgcgatga gtgggcaggg caactcggcg
     6121 acgagattct tgaccgtctc gatgacatag ggggtgtcga cgtccgcttc gagcgggcgg
     6181 agccggtcga ggtcggcggc ccgctcgaag gggcgctcga ccaccggccc gactccgggg
     6241 acgatgtcga ccccgaaccc gatggcgtga accggggtga cgatgtcgga atacaggatc
     6301 gcggcgtcgg tgccatagcg acgtaccggt tgcagcgtga tctcggtcgc caggtccggt
     6361 tgggcgatgg catgcagaat cgagcccgac ccgcgaatcg cccggtattc gggaagggag
     6421 cggcccgcct gtcgcatgaa ccacacgggg atgcggtcgt gtggttcgct gcgtgccgcg
     6481 gcgaggaatg gatcggatgg cgaagccggt gaggtcacca caccaagcta tcggtgctcg
     6541 ggggttcggc cgctacgggg agccgctcag atcgcggtcg ttgacgggcg aatgggtccg
     6601 ctagtctcgt ctacccccga ttttccatgg tgaccctccc ggagtgtttc gcgcatgccg
     6661 accaagtccg aactcgaggc cgaacaggcg tacatcgacc gtgcgtatga ctgcctcgag
     6721 cggacgcggg agcgtgcccg ctcggtcggc agcgaccccg aagcggatcg gggtggaact
     6781 tttcaggcgc gctacgaacg cgacgtgctc atcgaacgcg ctcagcatcg gctcgcccag
     6841 accgaccttg gcgaccagtc gttgtgcttc gggcggatcg acctcgaatc gggcgagcgg
     6901 tactacatcg gtcgcatagc ggtcagcgac gaacgtgccg aaccggtcgt ggtcgactgg
     6961 cgcgcgccgg tttccgagcc cttctaccga gctaccggca acgatccgat ggggttgtca
     7021 cggcgtcgcc acttcgcatc gtcgggcagg gtgctgcggg gcatcgagga cgagttcttc
     7081 ggcgaggccc gcgaccgtct cggcgacggt cgggtcaacg gtgagggcgc attgatcagc
     7141 gcgctcgaga ccgcacggtc gggcaagttg ggcgacatcg tcgcgacgat ccagggcgag
     7201 caggacgagg tcattcgtgc gccgctttcc ggggtgttgg tcgtgcaggg tgggccgggc
     7261 accggaaaga cggtcgtcgc actgcaccgg gctgcgtacc tgctctacac ccaccgcttc
     7321 cccctcgaag gccaaggcgt gttggtgctc ggtccgaacc gtctgttcct ctcctatatc
     7381 gagcaggtat tgccctccct cggcgaggct ggggtcgaga tcgccgtcat cgccgatctg
     7441 gttccgaacg ttcgggcgac cgggttcgac ccgccggagg cggcccgcct caagggcgac
     7501 cttcgcatgt gcaatgtgat cgccaaggcg gtgcgcgatc gtcaacgtcc gttgcgcgag
     7561 accgcggtca tcccgctcgg ggtcgaacgc ctccgcatca ccccggagga cacccgcgag
     7621 ttgatctcgc agacgcggcg gcgctcccgc acccacaacg gtggccgaaa ggcgttcttg
     7681 gaggggttct acgcgctggt ggcgtcgaag atgcgcgagc cgctcgaccc cggcgtcgta
     7741 cgcgaccgga tccggtcctt gcccgagacg cgcgagatcg tcgaacgggt ctggccgacg
     7801 ttgtcgccgg cgcaactact gaacgacctg ttttcctcga aaggcctgac tcgtctcgcc
     7861 gccaacaagt ggttgagcga caccgagcaa ctgctgttgt tgcgttcccg ggccgacgac
     7921 gcggacgaca tcgtctggag cgttcacgat gtcccgttgc tcgacgaagc gctcgaactg
     7981 ctcgggccgc gacccaaatt ccgcgagacc gagaccgtac gaacctatgg tcatatcgtc
     8041 gtcgacgagg cccaggacct gtcgccgatg gagttgcggg tcatcgatcg gcgatcgctg
     8101 aacggctcga tgacgatcgt gggcgacatc gctcaagcga cctcggcctc cgcccatgac
     8161 agttgggact cggtgctggc caacctgccg gccaagcgct cacctcggtt caccgagctc
     8221 gctatcggat accgggtgcc ggcgccggcg atggaactgg cgagtcgcgt gcttcgcctc
     8281 gcggcccccg gtctgcgccc accgaggtcg atccgagagg tcggcgacga gccgatcatc
     8341 gaggcgacta ccgtcgacag gttcgccgag gccttggcta ccgcggtccg acgcgaggtc
     8401 gcggaaatcg gcgcaggaag cgtcgcagtg atttgcgcca cctcgtggat cgcccgcgtc
     8461 gaggacgcat tgacgtcggg aggagtcgac ttcgggcggg cgcaccgcgg gcgattcgac
     8521 caccaggtca cggtcgcacc gatcacgctg gtgaaggggt tggagttgga cgccacgatc
     8581 gtgatcgagc ccgaggcggt gctcgaccag gagttccggg gcgcgcaggc gctgtatgtg
     8641 gggctcaccc gcgcgaccaa gcggttgtcg ttgctgcacg tcggcccgct ccccgacgtg
     8701 ctcgtcgaac cgctctagcc gctcgccggt tcgcccgatc ggccggccgc tcgccgacct
     8761 agccgtttgc cccgcgcagc caataggcca acgaggccgc gagcgggttg tcgtcgagcg
     8821 tggtctcgac atcgaagcgc accaccgggc gcgagccgtt gccgggcaga tgcggccagg
     8881 agtcgagtga tgcgatcgag gggatgccgt cacgggcgaa ggttgcccag gcgttcgaca
     8941 tggcatcggc gacctgctga ggtggctcgg gtccgaacag cggcgccagg cgggtgtcgt
     9001 gcagttgcga gaacacgaac ggcaactcca ccgcgtgtgc cgccccgagc atcgggctct
     9061 cccaatcgaa ctggtattgc cacaccggag cgtgtgacga atgtgcggca gccaattcgg
     9121 cggtcggtac tcgaaagacg atgtcggtga tgatcgcgca ttcgagctcg gcgggtgacg
     9181 cctcgggatg atcgatgcga taggcctcga tgagggtggc ggcgtcggcc ccgagcaggg
     9241 tggcgcgctc gacgagttgt tcctcggtct cagcgcccgg catgaggagc gcgaacagac
     9301 gccactcgtc gcggttgtgt ccgatcacca gcgggacacc ggctgcggcg ccttgggcga
     9361 tcgcggccag cgggtcggtc ggaacggcgt ggccgtcgcg gaccggtcga aaggccatgg
     9421 cagcgagaag ttcgcccgat tcggcgaggg tggtgttggg gtttccccat cgctgggcca
     9481 tgatcgtcgc gtggactgcg agcaggtcgg cgaggggcgc gttctgggct tcggtgacgc
     9541 tcgaccaccc gccaaggcgc atcacctcgg caccttcggt cctcgcgaat tcgaggtcgc
     9601 gggcggcatt cacgccaccg gactgacaga tcgctcggtg gaagagcccg tcggtgagcg
     9661 aggaactcat gagcagcgag gtgctcatcg acccggccga ctcgccgaac acggtcacgt
     9721 tgtcggggtc gccaccgaag cgggcgatgt tggtctgcac ccatcgcagc gcctcgatct
     9781 ggtcgagtag gccggcgttg ccggaaccga ggaattcgtc gtcgagacca ctgagttcga
     9841 ggaacccgag cgggccgagg cggtagttga tcgagacgaa cactgccccc tgtttggcga
     9901 acgcggtgcc gtcgtagatc gaggtcgacc cgctgcccat ctcgaaagcg ccgccgtgaa
     9961 tccagaacat gacgggcagt gcctcgtcgc cgctgagtcc gggatcgggg gtgtagatgt
    10021 tcagcctcag gcagtcctcg ctctgcggtt ccggttcgag gccgaacagc aggtccatga
    10081 acgaggggtt ctgcggtgcg atcggaccga attcggtggc gtcgaagacg ccctgccagg
    10141 ggtttcgggg ttgtggtgca cggaagcgaa gcgccccgac cggcgcctcg gcgtagggga
    10201 tgccggcgaa tcgggcgacc ccgtcgagcc actgcccccg caccgttccg gccgcggtgt
    10261 cgaccgtgag tgtgctgtga tcctgctggg tagtcatgga ccgatgctta ccagaatccg
    10321 ttcgctcgcg aaggctccga gcgcgacggt ggccccggcg atctcgacga cctgttcacc
    10381 tcggagtccg ctcgacacca cggttccctg cttgccgggt tgcagcgagt ggtcctcgag
    10441 gaagtccaac atgccgtcgg caaattcgag ttcttcgggg attcgacgca ccgtgaacgt
    10501 cgtgccgacg ggcaggttgg ccaacgcgat ggtctcgggg gggcgatagt tcgatcccgg
    10561 gatcgggttg ccgtgcgggc aggtcgacgg gtttcccagc agctcggcga ggcgttcctc
    10621 gacgagtggg ctgatgacgt gttcccactt gccggcctct tcgtgcgcga gcgcccaact
    10681 gagtcccaac acgtcggtga gcatgcgttc ggccagccgg tggcgacgca cgacggcctc
    10741 ggcgaggcgt tggccctcct cggtcagcgt gatcgagtcg ccgatgacga tgaggccttc
    10801 gttcgccatc cggcggatca tctccgacac cgctggtcgc gagatctcca ggcgctcggc
    10861 gatccgggcc tggatgacgt cgaggtcgtc ttcctggagt tcgaagatcg cctcgcaata
    10921 ctcctcgaag gccgggtgga actcgcctgc cttgttgccg cgcgacggtc tccgagcatt
    10981 cgtcatgtcc gcatgctatc tcgtgttagg aatgccgaac aacgggattc gctcgagcgg
    11041 tgccagacgt tcgctgtcgg tgcccggcgg tatcgttccg gccatgggcc tcgccgagtt
    11101 cagcgaaccg accacggcgt ggttcaccac caccttcggt caacccaccg aggcccaggc
    11161 gcgcgcgtgg cccgagatcg cggccggccg aaacgtcctg ttgtgtgcgc cgacgggttc
    11221 gggcaagacg ctggcggcgt ttctcgcggc gatcgaccgg ttggggtcga gtccggtttc
    11281 ccagaagggc tcgggcaagt cgatcgaacg aacgacggtg ctgtatctct cgccgatccg
    11341 cgcgctggcg gtcgacgtgg aaaagaacct gcgtgctccg attcgcggta tcaacctggc
    11401 ggcacagcgt ctcggtgtcg agtttcgtga gccgacggtc ggagtgcgca cgggcgacac
    11461 cccggcatcg ctccggcgtg cactcgtcaa ccatcctccc gacatcttga tcacgacccc
    11521 cgagtcgttg ttcctgatgc tgacgagcgc ggcccgagag tccctgcgct cggtcgacac
    11581 ggtcatcatc gacgagatcc atgcggtcgc cgcgacgaag cgagggtcgc acctggcgct
    11641 gtcgctcgaa cgccttcacg acctcgccga tgctgcacct caacgcatcg ggctgtccgc
    11701 gacgcagcgg cccctgtcgg aaatcgctca ctttctcggc ggcaacgagg ttgattccga
    11761 cggcgagctc cgtggccagc gagccgtgac gatcatcgac gaaggcggtc gcaaaccgct
    11821 tgaactcgaa gtggtcgtgc cggtcgacga catgggcggt gtcggtgagt cggacctcac
    11881 ccctcggtcg atctggccgt cggtgaacac ggcggtgctc gacctcatcg aggcccatcg
    11941 atccacgatc gtgttcgtca acgcccgtcg cctcgccgag cgcctcgcaa cccggctcaa
    12001 cgagttggag gaggaacgcg accgcaccgg cgacgtcgcg ctcgccgccg agcgcggcgg
    12061 cgagaacttc ggtgtgtcgc cgtccgcttc gggccacgaa ctggtgcggg cacaccacgg
    12121 ctcgctgagc cgcgagcagc gactcgtcgt cgaggacgag ctgaaggccg gcacgctgcg
    12181 cgggctggtc gccaccagct cgctcgaact cggcatcgac atgggtgcgg tcgatctcgt
    12241 cgtgcaggtc gcgtccccgg ggtcggtcgc gagtgggctg c
//
LOCUS       DAOOMA010000014        11610 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_6203_length_11610_cov_4.344007, whole genome shotgun sequence.
ACCESSION   DAOOMA010000014 DAOOMA010000000
VERSION     DAOOMA010000014.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 11610)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11610)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11610
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6203_length_11610_cov_4.344007"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            215..1003
                     /gene="proC"
                     /locus_tag="PLP95_00880"
     CDS             215..1003
                     /gene="proC"
                     /locus_tag="PLP95_00880"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HPB44387.1"
                     /translation="MTILQIVGGGKMGEALIGGILRGGDLAPGDVRVVEAVAERREAL
                     AELFPGAQISTEIGPASGTIIAVKPAGAAAVAAAVAAAGGGRILSIAAGVTLAQLEDA
                     AGPGVAVVRAMPNTPALIGEGASAVAGGTHASDADVDWAVGLLGAVGVVVRVDESQLD
                     AVTALSGSGPAYVFLIGEALIDAGVSAGLSRDLATTLAAQTLVGSAQLMAQSDLVPSE
                     LRAAVTSPGGTTAAGVRVLEQRAVRAALIDAVHAAFERSREMAH"
     gene            1000..1896
                     /locus_tag="PLP95_00885"
     CDS             1000..1896
                     /locus_tag="PLP95_00885"
                     /inference="COORDINATES: protein motif:HMM:NF014003.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent chlorinase/fluorinase"
                     /protein_id="HPB44388.1"
                     /translation="MSAGASRYDTVTLLSDLGTADGYVGVLHSIVRQLAPGAGVVDLT
                     HEIEACDVQAGALVLARSVQYLCPGVIVGLVDPGVGSDRRPIALEVTGGAAVLIGPDN
                     GLLASAVGMIGGAERAVVLDNVDYHLRSDATTFAARDIFAPVAAHLCAGVAFEDLGTL
                     VDPATLTPGLVPLNRTEQDADGTPVLEAEALWVDRFGNVQLNAELDDVAEFGDLLEVR
                     TTRGARTVSLVSTYSDLADGAIGLLVDSDGLLSLVTYGRSASTELVVSRGDAVSLRPI
                     SDDGTATSTTVALGPTRRLGGG"
     gene            complement(2054..2758)
                     /locus_tag="PLP95_00890"
     CDS             complement(2054..2758)
                     /locus_tag="PLP95_00890"
                     /inference="COORDINATES: protein motif:HMM:NF012415.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HPB44389.1"
                     /translation="MHLLRPRVSILIAALAIAVAGLILAPVHASAAPRAVLQWSSTDE
                     ERFVSKINELRTSLGLVPLNVDAELTERSRIWSETMRQAGSISHDPNPGTAMSADWKT
                     IGENVGVGGDVDALFEAFVRSPAHYANLINPEWRFVGVGVVMDGDRMFTTHRFMSLRP
                     PPAPTSPGASPAPGRRSPQPAPAPAPAPAPAPVPAPEPVPASPPNDPASVPPPSPVEP
                     RRLELLLSAIGDDVNG"
     gene            2959..4449
                     /locus_tag="PLP95_00895"
     CDS             2959..4449
                     /locus_tag="PLP95_00895"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HPB44390.1"
                     /translation="MNLAAIFDRHDPDNVALISRGRPTTYGELGSLVAGVRGGLAAQG
                     LVPGDRVAIICGNNIYFVASYLAAVGAGMIAVPLNPLSPPVELVAQLSTVSARAAVVG
                     PTARSVFEQAEDNPLTSVDVVIGCGFLPDGGLTFDDLAESDPAPIVERDDDDVALMVF
                     TSGTAGAPKAAMLTHGNLRSNIAQALSIRENAYTADDVFFGILPMFHIFGLNVVLGLA
                     FAIGARVLLIERFDPVSAIEAIEKHAVTVVTGPPTMWSAFANMRGVSPDSFNSIRVAV
                     SGAARLPEETGRAFEARFGVRIQEGYGLTEASPIVTASMGTDAPYGSVGVPVPGLEVR
                     LIDESGDDVLIGDPGELLVRGPNVFKGYWNDDAATASVIDADGWLHTGDVATVDDDGF
                     IYLVDRVKDLIIVSGFNVYPAEVEEVLITHPSIDACAVVGVPHPYSGEAVKAYVVGVK
                     GSSIEEDEVVAYCADHLARYKCPDKVWFVDEVPVGLGGKVLRRALR"
     gene            4528..5178
                     /locus_tag="PLP95_00900"
     CDS             4528..5178
                     /locus_tag="PLP95_00900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0051775 - response to redox state [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HPB44391.1"
                     /translation="MGGSSEHSIPEATVSRLPLYLRSLQELVDDGMATVSSEGLAELA
                     GVNAAKVRKDLSYFGSYGTRGVGYEVEYLLRHIRAQLGLTHDWPCAIVGLGNLGQALA
                     NYGGFSDRGFVISALVDADPDKVGTELYGITISPVEDLAALVAEHGISIGIIATPAAA
                     AQDVADRMVEAGITSILNFAPTTIATPVDVQVRKVDLAVELQILSFYQERRDTADS"
     gene            5300..6565
                     /gene="hemA"
                     /locus_tag="PLP95_00905"
     CDS             5300..6565
                     /gene="hemA"
                     /locus_tag="PLP95_00905"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: protein motif:HMM:TIGR01035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008883 - glutamyl-tRNA reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HPB44392.1"
                     /translation="MSTIVAIGATHRSAPLALLERLSFDEAQLAKYLGDLVERDHINE
                     AVILSTCNRVEIVVSAEKFHGGYQDVRNFISDVTFLLPEEFVDSLVVTHDADAVRHLF
                     DVAAGLDSVVIGEHEILGQVRNAWQAAQEVGSVGPTLNLLFRNAVEVGKRARNETAIS
                     RHVTSVSHASVIMAERHLGGFPGRTAAVVGAGSMARGVVDFLAADGADEIIVLNRTPG
                     RGAQLGAAVSRSGGLDQLHSVLATVDAVFFATAAPRHVLSYRELEDAMAVREGRELLL
                     VDIAMPRDVEPEVSQIPGVSLLDMDALSRFAAEGLSKRRREIPAVRLLVEEQLERFLS
                     ETSAREVAPLIVELRRRTEQIVAAERDRHASKFASLDDEQLAAVDALLNSTVGKLLHE
                     PILALKSSAGSPRGDRLVGTLRELFGLSE"
     gene            6571..7476
                     /gene="hemC"
                     /locus_tag="PLP95_00910"
     CDS             6571..7476
                     /gene="hemC"
                     /locus_tag="PLP95_00910"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: protein motif:HMM:TIGR00212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004418 - hydroxymethylbilane synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HPB44393.1"
                     /translation="MRLPHTITLRIATRASALALWQANHVAELLAVQAEARGLLPEVE
                     LVTVSTEGDRRLDVALSAIGGKGVFVKEVQAAVLDGRADLAVHSAKDLPALTPDGLVI
                     AALPERADPRDALVGVRLDDLAAGATVATGSARRRAQLAALRPDLGFVELRGNIDTRL
                     AKVGEVDAIVVAAAALDRLGRTPEVVDRLGVDTMIPQVGQAALAVETRADDTDLTALV
                     AQLDHADTRRCVTAERSFLETLGGDCELPAGAYATLVDGAVRLRAMLLAVETGEVCRT
                     ELVGEDPVEMGTNAAHELSQRVELR"
     gene            7486..9021
                     /gene="cobA"
                     /locus_tag="PLP95_00915"
     CDS             7486..9021
                     /gene="cobA"
                     /locus_tag="PLP95_00915"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: protein motif:HMM:NF004790.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004851 - uroporphyrin-III
                     C-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0019354 - siroheme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HPB44394.1"
                     /translation="MTVYLVGAGPGDPGLLTVRGAEVLRRADVVIYDRLSAVSLLDLA
                     PPDALRVNVGKHGSGGHSVSQDRINQLLVEHGRGSATVVRLKGGDPFVFARGAEELAV
                     LREAGIAAEVVPGITSAIAAPAYAGIPVTRRYSSTSFTVVTGHEDPTKGRTDVDWESI
                     AKVGGTIVVLMGVANIAQISERLLAGGLSPDTPAAAVRWGTRPEQSVVRATLATIAEQ
                     KLAAPSVIVVGDVAGDALDWFTDRPLFGRTVVVTRAAHQASRLTQRLSDAGAAVVGVA
                     TIEIHDPADGGSALAAALERIDGYDWLVVTSPNGATRVLDALGDVRRLAGVGVAVIGP
                     GTAEVFRSARIEPDLIPARYVAEGLLEAFPPAPEGGGRVLVAQAATAREVTVAGLRDA
                     GWEVDVVAAYRTVPATLDDSRRADIARADAVAFASSSAVKGFVAGVPADSWPRTAVAI
                     GPLTAATALEAGFGRVVESAVHDLDGLVETVIDAVGGGGAAAAGGAGAAGAAGAASDP
                     VER"
     gene            9027..10031
                     /locus_tag="PLP95_00920"
     CDS             9027..10031
                     /locus_tag="PLP95_00920"
                     /inference="COORDINATES: protein motif:HMM:NF015338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HPB44395.1"
                     /translation="MGSVLAVLPRPDGAWTVVRVLAWASLVPMVLVSLWIHFGLSRPS
                     PTLVLTGFQMVLLAPAWPILVGAVIGRRWGMAAVAAFVAVSHLWFCVPAVTADTAPGW
                     VREAPTFTMYSANVLYRSERVDEMAAKVMEVDTDVVVLNEMTDPLRAALEEAGVFERY
                     DTEVYWRGRPFGEMLLTRLPVIDSGVSNLARMGVPWATVQVGDAQVRVHTVHIQAPKN
                     RDDRHKWRANLDAVGREVEARDGLAMVFAGDFNSTVWHGPFRRLLDRGLTEAHGQLGQ
                     GLSRSWTSPVVPLRWLGPIMRLDHVLLSEGAWVVEVRDTDTPGSDHRGIISTLAVRPT
                     "
     gene            complement(10039..11205)
                     /locus_tag="PLP95_00925"
     CDS             complement(10039..11205)
                     /locus_tag="PLP95_00925"
                     /inference="COORDINATES: protein motif:HMM:NF013613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leishmanolysin-related zinc
                     metalloendopeptidase"
                     /protein_id="HPB44396.1"
                     /translation="MKRRHVALAFALPLAMMSAPGCIPDNPPVTVPPVTASPQASKIQ
                     SFTASASSGKAPLSIALSWSVSGTGPLTCELDTDGNGSVDITITDCPSAGSRPTTLGS
                     VGTHAPVIRVSGPGGVATRTTTITTSAAGDDRFDIDIVPLEGAGAYQSEIDAAVARWE
                     AVIHNGLNDSVLKLPADYCWSKGRPAEDLSVDDLAIEIKIAPDDGPGGRLGSAGFCYA
                     TGGMPRYGAINLDVDDVARLRASGRLVDALTHEIGHVLGIGTAWNFGGRTLLVGAGTG
                     DPRFSGPVAVAAWATLGGSAAVPVESEGGEGTADAHWRESTFDDELMTGWLSGPVRPL
                     SIVTVASLADLGYSVDLNGADPFSTAPSMRAFGLNGERHEEVAHVELFNPQNRP"
     gene            11294..>11610
                     /locus_tag="PLP95_00930"
     CDS             11294..>11610
                     /locus_tag="PLP95_00930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012229609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HPB44397.1"
                     /translation="MEASQFPDRRLRRLRRSAPMRRLVAETVLTPNDLIAPLFVREGI
                     DSPVEISSLPGVFQLDRRSMIDEVGQLVDLGVPAVMLFGVPERKDPMGSGADDPDGIV
                     QVA"
BASE COUNT         1673 a         3538 c         4356 g         2043 t
ORIGIN      
        1 tgtcaccgtt gttggtgtgt tgggatgccc tgtcgtgctc gaacatgtgt tcatgatatc
       61 gaacgaaagt tcggtttgca agacgaaacc ccacgaatcc agaacaaatg ttcgccacct
      121 cgatatgcac ctccgagcgc cgcttacagc gcccaccaac acgccgccgc cacccacatg
      181 cagcctccgg catcggctcg cgtaggctga ccgcatgacc atcctgcaaa tcgtcggcgg
      241 cggaaagatg ggcgaggccc tgatcggcgg catccttcgc ggcggcgatc tggcaccggg
      301 agacgttcgg gtcgtggagg cggtggccga gcgtcgtgag gcactcgcag agctgtttcc
      361 cggtgcgcag atctccaccg aaattgggcc ggcctcgggc acgatcatcg ccgtgaagcc
      421 cgccggggcg gcggcggttg cggcggcggt ggccgcggcg ggaggcggca gaatcctctc
      481 gatcgccgcg ggggtcaccc tcgcgcaact cgaggatgcc gccggccctg gcgttgcggt
      541 ggtgcgggcc atgccgaaca cccccgccct gatcggcgag ggcgcctcgg ccgtcgccgg
      601 gggaacccat gcttccgacg cagacgtcga ctgggcggtg gggctgctcg gcgcggtcgg
      661 agtggtcgtt cgggtcgacg agtcgcaact cgatgcggtc acggcgttgt cgggttccgg
      721 cccggcgtac gtcttcctga tcggtgaggc gttgatcgac gccggggtgt cggcagggct
      781 ttcccgcgac ctggccacga ccctcgccgc tcagaccctg gtgggttcgg cccaactgat
      841 ggctcaaagc gacctggtgc cctcggagct tcgcgccgcg gtcacttcgc ccggcgggac
      901 gacggcggcc ggagtgcggg ttctggaaca acgcgccgtg cgggcagcgc tcatcgatgc
      961 ggtgcacgcc gcgttcgaac gctctcggga gatggcacat tgagcgcggg ggccagtcgc
     1021 tacgacaccg tgaccctgct gagcgatctg ggcaccgccg acgggtatgt cggagtgctg
     1081 cactcgatcg ttcgacagct tgcgccgggg gccggtgtgg tcgatctcac tcacgagatc
     1141 gaggcctgcg acgtccaggc cggagccctc gtgttggcac gaagcgtgca gtatctgtgc
     1201 ccgggggtga tcgtcgggct cgtcgacccg ggggtcggct cggatcggcg gccaatagcg
     1261 ctcgaagtga cgggtggcgc tgcggtcctg atcggtcccg acaacgggtt gttggcctcc
     1321 gcggtgggca tgatcggtgg cgcggagcgg gcggtcgtgc tcgacaacgt cgactatcac
     1381 ctccggagcg acgcgacgac ctttgccgct cgcgacatct tcgctccggt ggcggcgcat
     1441 ctgtgcgccg gcgtggcatt cgaggacctc ggcacgctcg tcgacccggc cacgctcacc
     1501 ccggggctgg tgccgctcaa ccggaccgag caggacgccg acgggacgcc ggtgctcgag
     1561 gccgaggcgc tgtgggtcga tcggttcggc aacgttcagc tcaacgccga actcgacgac
     1621 gtagccgagt tcggcgacct gctcgaggtg cgaacgactc ggggagcccg cacggtatcg
     1681 ctcgtttcga cttattccga cctcgcggac ggagcgatcg ggctgctcgt cgacagcgac
     1741 ggactgttga gcctcgtgac ctacgggcgt tctgcctcga cggaactcgt cgtgtcgagg
     1801 ggcgacgcgg tgtcgctgcg tccgatctcc gacgacggca ccgcgacctc gacgacggtt
     1861 gcactcggtc cgactcggcg cctgggaggt ggatgatgcg caaaggaacg gcgatcgtga
     1921 tcatcgtgtt gctggcggtg ctggtggcgg cgaccgcgct gcagttgtcg atggtgtcgc
     1981 gctgaggact gcgccctgag gaccgtcgcg ctgagggctg ccgctgagga ctgtcgcgct
     2041 gagggccgga ggttcaaccg ttcacgtcgt cgccgatggc gctgagcagc aactccaggc
     2101 ggcgcggctc gaccggagat ggcggcggca ccgaggctgg atcgttcggc ggggaggcgg
     2161 gaaccggttc aggcgccgga actggagccg gagccggagc cggggcagga gcgggcgcgg
     2221 gttgcggtga gcgccgacct ggtgctggcg acgcgccggg tgaagtcggg gcgggcgggg
     2281 gccgcagcga catgaatcga tgcgtggtga acatgcggtc gccgtccatc acgaccccca
     2341 cgcccacgaa acgccactcg gggttgatga ggttggcgta gtgggcaggg ctcctcacga
     2401 acgcttcgaa cagggcgtcg acgtcgccgc ccacaccgac gttttcgccg atcgtcttcc
     2461 aatcggcgct catcgccgtg ccggggttgg ggtcgtggga gatcgaaccg gcctgacgca
     2521 tcgtctcgga ccagatccgc gaacgttcgg tgagttccgc atcgacgttc aacggcacga
     2581 ggcccagcga cgtgcgcaac tcgttgatct tggagacgaa tcgttcctcg tcggtgctgg
     2641 accattgcaa cacggctcgg ggcgcggccg aggcgtggac cggagcgagg atgaggccgg
     2701 caacggcgat cgcgagcgcg gcgatcagta tcgagactcg tggtcgaagc agatgcatcg
     2761 tcgatatggc ccagcgttgc gacgtgcccg ggtggcggcg tctcaaggtt gatcgggcac
     2821 ggcgtcgatt tccttgagaa aggcgcgaac gccggggctg ggtcgaacgc cccgcgccgg
     2881 accgaaacta cacattcgac gaaggtgagc gatcgcgggc gctaccctga cacaacgccc
     2941 gaaccgtggg gaggcgctgt gaaccttgca gccatcttcg atcgtcatga ccctgacaac
     3001 gtcgcgttga tcagtcgcgg tcgcccgacg acctatggcg agttgggttc gctggtcgca
     3061 ggagtgcggg gcgggttggc tgcgcagggg ttggttccgg gagatcgggt cgccatcatc
     3121 tgcggcaaca acatctattt cgtcgcgtcg tacctggccg cggtcggggc cggcatgatc
     3181 gcggtgccgc tcaatccgtt gagccctccg gtcgagttgg tcgcccagct ttcgacggtg
     3241 tcggcacggg cggcggtggt ggggccgacg gcacgctcgg tattcgaaca ggccgaggac
     3301 aacccgctca cgtcggtgga cgtcgtcatc ggttgtgggt ttctccccga cggcgggttg
     3361 accttcgacg acctcgcgga gtcggatccg gcgccgatcg tcgaacgcga cgacgatgac
     3421 gtggcgttga tggtgttcac cagcgggacg gccggcgccc cgaaggccgc catgttgacc
     3481 cacggcaatc tgaggtcgaa catcgctcag gcgctgtcga ttcgcgagaa cgcctacacc
     3541 gctgacgacg tgttcttcgg catcttgccg atgtttcaca tcttcgggct caacgtcgtg
     3601 ttgggcctgg cgttcgccat cggcgcccgg gtgctgttga tcgagcggtt cgacccggtc
     3661 tctgcgatcg aagcgatcga gaagcacgcg gtcacggtcg tgacggggcc gccgacgatg
     3721 tggtcggcct ttgcgaacat gcggggggtt tcgccggatt cgttcaactc gattcgggtg
     3781 gcggtgtcgg gcgcggcgcg cctaccggag gagaccggac gggcgttcga ggcgcgattc
     3841 ggggtgcgga ttcaggaggg atacggcctg accgaggcgt cgccgatcgt taccgcgtcc
     3901 atgggcaccg atgcgccgta cgggtcggtc ggagtgccgg tgccgggcct tgaggtgcgc
     3961 ctgatcgacg agtcgggcga cgacgtgttg atcggagacc cgggggaact gttggttcgc
     4021 gggccgaacg tgttcaaggg ctattggaac gacgatgcgg cgaccgcgtc ggtgatcgat
     4081 gccgacggat ggttgcacac cggcgatgtg gcgaccgtcg acgacgacgg cttcatctac
     4141 ctggtcgatc gggtgaagga cctcatcatc gtgtcggggt tcaacgtcta tccggccgag
     4201 gtggaagagg tgctgatcac ccatccgtcg atcgatgcat gtgcggtggt gggggttcct
     4261 cacccctatt cgggtgaggc cgtgaaggcc tatgtggtgg gggtgaaggg gtcttcgatc
     4321 gaggaggacg aggtggtggc gtactgcgcg gatcacctcg cccgttacaa gtgcccggac
     4381 aaggtgtggt tcgtcgacga ggtgccggtc ggcctcggcg gaaaggtgct tcggcgggcg
     4441 ctgcgctgaa ggttcgcgcc gtcggcggcg ttccgtggcg tctcgccaca ttgtgaattc
     4501 gtgcacgaga tcgctagctt gggcgtcgtg ggtggatcgt cggaacactc gatccccgag
     4561 gcaaccgtgt cacgcctgcc gctgtacctg cggagtctgc aggaactggt ggacgacggc
     4621 atggcgacgg tgtcatcgga gggcttggcg gaactcgccg gggtcaacgc tgccaaggtt
     4681 cgcaaggatc tgagttattt cgggtcgtac ggcacccgcg gggtcggcta cgaggtcgag
     4741 tacctgctgc gccacatccg ggcccaactc gggcttaccc acgactggcc gtgtgcgatc
     4801 gtcgggttgg gaaatctcgg tcaggccctc gccaactacg gcgggttcag cgaccggggc
     4861 tttgtgattt cggccctcgt ggacgccgac ccggacaagg tcggaacgga gctctacgga
     4921 atcacgatct ccccggttga agacctggcg gcgttggtgg ccgaacacgg catcagtatc
     4981 gggatcatcg ccacgccggc ggctgcggcc caagacgtgg cggaccgcat ggtcgaggcg
     5041 ggcatcacct ccattttgaa cttcgcgccg acgacgatcg ccaccccggt cgacgttcag
     5101 gtgcgaaaag tcgacctggc ggtggagttg cagattctca gcttctatca agagcgtcgc
     5161 gacaccgccg actcgtgacc gccgactcgc cacgccgaac agccaagccg ttcggggcac
     5221 cgttttgatc ggctccttga cacggtgttc aatactcccc atgggttcct gacaccccga
     5281 ggaaagccca ggtgatgccg tgtccaccat cgtggcaatc ggagcaacac atcgaagtgc
     5341 gccgctcgcc ctgctcgaac gactgtcgtt cgacgaggcg cagctcgcca aatatctcgg
     5401 tgacctcgtc gagcgcgacc acatcaatga ggccgtgatc ctgtcgacgt gtaaccgcgt
     5461 cgagatcgtg gtgagcgccg agaagttcca tggtggctat caggacgtgc gcaacttcat
     5521 cagcgacgtg acgttcctgc tgcccgagga gttcgtcgat tcgctcgtcg tcacccacga
     5581 tgccgacgcg gtccgccacc tgttcgacgt cgctgccggc ctcgattcgg tggtgatcgg
     5641 cgaacacgag attctcgggc aggtgcgaaa cgcctggcag gcggctcagg aggtcggctc
     5701 ggtcggtccg acgttgaacc tgctgtttcg caacgcggtc gaggtcggta agcgggcgcg
     5761 caacgagacc gcgatttcgc gtcacgtcac gtcggtgagc catgcctcgg tgatcatggc
     5821 cgagcggcac ctcggcgggt ttcccggccg gaccgcggcg gtggtcggtg cggggtcgat
     5881 ggcccgcggg gtggtcgact tcctggccgc cgacggtgcg gacgagatca tcgtgttgaa
     5941 ccgcaccccg ggccgcggcg cgcagttggg tgcggcggtg agccgcagcg gcggactcga
     6001 tcagctgcat tcggtgttgg cgacggtcga cgcggtgttc ttcgccaccg cggcgccgcg
     6061 tcacgttctg tcgtatcgag aactcgaaga cgcgatggcg gttcgcgagg gccgggaatt
     6121 gttgctggtc gacatcgcga tgccgcgcga tgtcgagccg gaggtgtcgc agatccccgg
     6181 ggtgtcactg ctcgacatgg acgcgttgtc gcggttcgcc gccgagggcc tgtcgaagcg
     6241 acgtcgcgag atcccggcgg tgcgcctgct agtcgaggag cagctcgaac ggttcttgtc
     6301 ggagacctcc gcacgagagg ttgcgccgtt gatcgtcgag ttgcgtcggc ggaccgaaca
     6361 gatcgtcgcc gccgagcgcg atcgccacgc ctccaagttc gcctcgctcg acgacgagca
     6421 actcgcggcg gtcgacgcgc tgttgaactc gacggtgggc aagttgttgc acgagccgat
     6481 cctggcgttg aagagttcgg cgggatcccc gcggggcgat cgtctcgtcg ggacgttgcg
     6541 cgagctgttc ggcctgtcgg agtagcgtcg atgcggctgc cccacacgat taccctccgc
     6601 atcgcaacac gcgcgtcagc gctggcgctg tggcaggcga atcacgtcgc cgagctgttg
     6661 gcggtgcagg cggaggcccg tggcctgctg cctgaggtcg agctggtgac ggtgagcacc
     6721 gagggcgacc ggcgcctcga cgtcgcactg tcggcgatcg gcggcaaggg ggtgttcgtc
     6781 aaggaggtgc aggcggcggt gctcgatggc cgcgccgatc tggcggtgca ttcggccaag
     6841 gatctcccgg cgttgacacc cgatgggctg gtcatcgctg cgcttcccga acgggccgac
     6901 cctcgcgacg cgctcgtggg ggttcgcctc gatgacctgg cggcgggtgc gacggtggcg
     6961 acggggtcgg cgcgacggcg ggcccagctc gctgcgttgc ggcccgatct tggcttcgtc
     7021 gagcttcgcg gcaacatcga tacccggctg gcgaaggtgg gtgaggtgga cgcgatcgtg
     7081 gtggcggcag ccgcgctcga tcggctgggc cgaacccccg aggtggtcga ccggttgggg
     7141 gtcgacacga tgattccgca ggtcggtcag gcggcgttgg cggtggagac ccgtgccgac
     7201 gacaccgacc tgacggcgtt ggtcgcgcag ctcgaccacg ccgacactcg aaggtgtgtg
     7261 acggcggagc gttcgtttct cgagaccctc ggcggcgatt gtgagcttcc ggccggggcg
     7321 tacgcaacgc tcgtcgacgg tgcggtgcgc ctccgggcga tgctgttggc ggtcgaaacg
     7381 ggtgaggtgt gtcgaaccga gctcgtcggt gaagatcctg tcgagatggg cacaaacgcg
     7441 gcgcacgagc tgtcgcaacg ggtcgagctc cgctaaacca cgatcatgac cgtctacctc
     7501 gttggcgctg gccccggtga tcccggattg ctcaccgttc gcggggccga ggtgctgcgt
     7561 cgggccgatg tcgtgatcta tgaccggttg tcggcggtgt cgctgctcga tctcgccccg
     7621 ccggatgcgc ttcgggtgaa cgtcggcaag cacggatcgg gcgggcacag cgtcagccag
     7681 gatcggatca accagctgct cgtcgaacac ggtcgcggct ccgccacggt ggtgcgtctc
     7741 aagggcgggg acccgttcgt gttcgcccga ggtgccgagg agcttgcggt gctgcgcgaa
     7801 gccgggatcg ccgccgaggt ggtgccgggg atcacgtccg cgatcgccgc tcccgcctac
     7861 gcgggcatcc cggtgacccg ccggtactcg tcgacgtcgt tcacggtggt gacgggccac
     7921 gaagacccga ccaagggccg aaccgatgtc gactgggaat cgatcgcgaa ggtcggcggg
     7981 acgatcgtgg tgttgatggg ggttgcgaac atcgcgcaga tctccgaacg cctgctcgcc
     8041 ggggggctgt ccccggacac ccctgcggcg gcggtgcggt gggggactcg ccccgaacag
     8101 agcgtggtgc gcgcgaccct ggcgacgatc gccgagcaga agctggcggc gccctcggtg
     8161 atcgtggtgg gcgacgtggc aggcgacgcg ctcgactggt tcaccgaccg tccgttgttc
     8221 gggcgcaccg tggtggtgac tcgcgccgca catcaggcct cgcgtctcac gcaacggttg
     8281 agcgatgcgg gagccgcggt cgtgggggtg gcgacgatcg agattcacga tccggccgat
     8341 gggggatcgg cgttggccgc tgcgctggaa cggatcgacg gctacgactg gctggtggtg
     8401 acctcgccga acggggcgac ccgcgtgctc gacgcgctcg gcgatgtgcg tcgcctcgcc
     8461 ggggtcgggg ttgcggtgat cgggccgggg accgccgagg tgtttcgctc ggcgaggatc
     8521 gaaccggatc tgatcccggc gcggtacgtg gccgaggggt tgctcgaggc gtttccgccc
     8581 gccccggagg gtgggggccg ggtgctggtg gctcaggctg cgacggcccg ggaggtgacg
     8641 gtggcggggt tgcgtgacgc cgggtgggag gtcgacgtcg tcgccgccta ccgcacggtg
     8701 ccggcgacgc tcgacgactc gcggcgcgcc gacatcgccc gtgccgacgc ggtggcgttc
     8761 gcgtcgtcat cggcggtgaa ggggttcgtc gccggggtgc ccgccgactc gtggccgcga
     8821 acggccgtgg ccatcggacc gctcaccgcg gcgacggcgc tcgaggccgg gttcggacgc
     8881 gtggtcgaaa gcgcggtgca cgacctcgat ggcctcgtcg agacggtgat cgatgccgtc
     8941 gggggtggag gtgccgccgc ggctggcggg gctggcgcgg ctggcgcggc tggcgcggcg
     9001 tcggacccgg tcgagcgctg acggcgatgg gatcggtgtt ggcggtgttg ccgcgtcccg
     9061 atggggcatg gacggtggtg cgggtgctcg cctgggcgag cctcgtgccg atggtgctgg
     9121 tgagcctgtg gattcacttc gggttgagcc ggccgtctcc gacgttggtg ttgaccggct
     9181 ttcagatggt gttgttggct ccggcgtggc cgattctcgt cggtgcggtg atcggccggc
     9241 gatggggcat ggccgcggtg gcggcgttcg tggcggtgtc gcacctgtgg ttctgtgtcc
     9301 cggccgtcac cgcggatacc gcaccaggat gggtgcgcga ggcccccacg ttcacgatgt
     9361 acagcgcgaa cgtgctgtat cgaagcgaac gggtcgacga gatggccgca aaggtcatgg
     9421 aggtcgacac cgacgtggtc gtgctcaacg agatgaccga tccgctgcgg gcggcgcttg
     9481 aggaggcggg ggtgttcgag cggtacgaca cggaggtgta ttggcgcggg aggcccttcg
     9541 gcgagatgtt gctgaccagg ttgccggtga tcgattcggg ggtgtcgaat ctcgccagaa
     9601 tgggcgttcc gtgggccacg gtgcaggtgg gcgacgctca ggtgcgggtg catacggtgc
     9661 acattcaggc gccgaagaat cgagatgatc gccacaagtg gcgggccaat ctcgacgccg
     9721 tcgggcgcga ggtggaggcg cgcgatggcc ttgcgatggt gttcgccggc gacttcaaca
     9781 gcacggtgtg gcacgggccg tttcggcgtc tgctcgatcg ggggttgacc gaggcccacg
     9841 gccaactcgg ccaggggttg tctcgttcgt ggacctcgcc ggtggtgccg ttgcgctggc
     9901 tggggccgat catgcgtctc gatcacgtgt tgctgagcga gggcgcctgg gtggttgagg
     9961 tgcgcgacac cgacacgccg ggcagcgatc accgcgggat catttcgacg ctggcggtgc
    10021 gcccgacctg actcgtggtc aggggcggtt ttgtgggttg aacaactcca cgtgggcgac
    10081 ttcctcgtgg cgctcgccgt tgaggccgaa cgctcgcatc gatggggcgg tcgagaacgg
    10141 atcggcgccg ttgaggtcga ccgaatagcc gaggtcggcg agcgacgcga ccgtcacgat
    10201 gctgagcggg cgcaccggcc cggacagcca accggtcatc aactcgtcgt cgaaggtgga
    10261 ttcgcgccag tgggcgtccg cggtgccctc gccgccctcg ctttcgaccg gcaccgcagc
    10321 ggagccgccc agggtggccc aggccgcgac ggcgacgggt ccgctgaagc gcgggtcgcc
    10381 ggtgccggcg ccgaccaaca gggtgcggcc gccgaaattc caggcggtgc cgatgccgag
    10441 cacgtggccg atttcgtggg tgagggcgtc gacgagacgg ccgctggcgc ggagtcgagc
    10501 gacgtcgtcg acgtcgaggt tgatggcccc gtatcggggc atgccgccgg tcgcataaca
    10561 gaatccggcg ctgccgaggc gtccgcccgg gccgtcgtcg ggcgcgatct tgatctcgat
    10621 cgcgaggtcg tcgacgctca agtcctcggc cggccggcct ttgctccagc agtaatcggc
    10681 gggcagtttc aggaccgagt cgttgaggcc gttgtggatg accgcctccc atcgagcgac
    10741 cgctgcgtcg atctcgctct ggtacgcgcc ggcgccctcg agggggacga tgtcgatgtc
    10801 gaagcggtcg tcacccgccg cgctggtggt gatcgtggtg gtcctggtgg cgaccccgcc
    10861 ggggccggag acccggatga cgggggcgtg ggtgccgacc gaacccagcg tggttggccg
    10921 gcttccggcg ctcggacagt cggtgatggt gatgtcgacc gacccgttgc cgtcggtgtc
    10981 gagctcacag gtcagcggcc cggtgccgga caccgaccat gacaaagcga tgctgagcgg
    11041 ggctttgccg ctcgaggccg acgcggtgaa cgactggatc ttggacgcct gaggcgaggc
    11101 ggtgaccggt gggacggtga cgggtgggtt gtcggggatg cagccggggg cgctcatcat
    11161 ggcgagcgga agtgcgaatg cgagggcgac atggcgacgc ttcatctgct gtgtatcgac
    11221 acgttcgagt cgcgacgtga gggatttctt accaacgggt cggtgctggc ggcggcgttc
    11281 gtagactctg ggcgtggaag cctcccaatt tcccgaccga cggctgcgtc gccttcgccg
    11341 aagcgccccg atgcgtcgac tcgtcgccga gaccgtgctc accccgaacg acctcatcgc
    11401 cccgctgttc gtgcgcgagg gcatcgactc gccggtggag atctcctcgc tgccgggggt
    11461 gttccagctc gatcggcgct cgatgatcga cgaggtcggc cagctggtcg acctgggtgt
    11521 gccggcggtg atgttgttcg gggttcccga acgcaaggac ccgatgggtt cgggtgccga
    11581 tgaccccgac gggatcgttc aggtcgcgtt
//
LOCUS       DAOOMA010000015        11570 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_6239_length_11570_cov_4.431437, whole genome shotgun sequence.
ACCESSION   DAOOMA010000015 DAOOMA010000000
VERSION     DAOOMA010000015.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 11570)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11570)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11570
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6239_length_11570_cov_4.431437"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..865)
                     /locus_tag="PLP95_00935"
     CDS             complement(<1..865)
                     /locus_tag="PLP95_00935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB44398.1"
                     /translation="MLESGNPKTMGRAAAAGVVSSASTAYDPEEVFTALRTLRLGGTD
                     TASRHLKLPPEAHAALPPAITAARWAAIAYGMIFGAQQAFGGSYSVVVSLTVCLFLTS
                     WRSIIPTQLGAPDRGAQIVALIDVAIFGLGVGISAGPTSPFIFCFLVALAVPALGWGY
                     RLGALGWCLGWTVTLMSTMAFGGDLGDAFLQEFLVMAIAAVLVVAGSAFVRSRLIESH
                     ERRLAMSGQMLALSEANDLLTMLNSVARTLPASLSQRDVLDNARQQLVNTFHNRVLCL
                     FGFPVYPEFARV"
     gene            1057..2028
                     /locus_tag="PLP95_00940"
     CDS             1057..2028
                     /locus_tag="PLP95_00940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HPB44399.1"
                     /translation="MESTTDSIDEKSLGVLAGSEAAAVQIPGARRPDRRTSLDVGGLS
                     ISRVEWGPPDAPPLLFAHGGFDFAETLNVFAPKLVERGWRVVSWDHRGHGHSEWAQLY
                     SLEADIRDAAFVVESVSAERFPLIGHSKGGAMCLQLAVLFPHKFTHLVNLDGLPSRKT
                     MPDVAEHQRIRMVASELENWLGHRASLVDRQRPAGTIAELAQRRGRMNTRATTEWLQY
                     LVTVGGRRDVDGWRWRIDPTLRMGGFGPWRPEWSMYRLPEVTMPLLAILCGEPELMGW
                     GTRAEDVEPYLPIGAELMELAGIGHFMHIEQPDLLVQIIGSFIGDPQ"
     gene            2025..2672
                     /locus_tag="PLP95_00945"
     CDS             2025..2672
                     /locus_tag="PLP95_00945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HPB44400.1"
                     /translation="MITRLHHGRVELALHRLRDGDTTTPLLLLHPLGSRTPEVAPNWS
                     DRWPGPVFGLDFTGHGASTVPRGGGYSAEVLLADVDTALTHLGAVSIVGRGLGGYVGL
                     LAAGARPSGVRGVVIDDGPGLTGGGTVPSSPSFVAPVFGSSSSPDPFALQELSRDIRP
                     PDYVRNFARFALEGSPAAEPIIVSARILPAWLEAVADEHGVVRCSFEAAVERLGR"
     gene            complement(2714..2950)
                     /gene="xseB"
                     /locus_tag="PLP95_00950"
     CDS             complement(2714..2950)
                     /gene="xseB"
                     /locus_tag="PLP95_00950"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR01280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HPB44401.1"
                     /translation="MTTTSPPSSGSSDESIGYVDAMAELEAILAELDRPDVDIDILAD
                     RVERASQLVSLCRERLERARLRVTEIVADLETDD"
     gene            complement(2947..4269)
                     /gene="xseA"
                     /locus_tag="PLP95_00955"
     CDS             complement(2947..4269)
                     /gene="xseA"
                     /locus_tag="PLP95_00955"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HPB44402.1"
                     /translation="MTETTTWKIPQLIDWVNAVVANSLGGEVWVEGEISNLQRSAAGH
                     VYFTLIERDLDERQRSPHTLAVTLFEWHRVNVNRHLKRSGGSVRMGDGVRVRIRGTVE
                     VYSARSQLQLRMSGIDPVFTLGSLAADRARLLAALQDEGLLEAQQRLETPPLPLRVVI
                     ITSIGSAAHADALDELRQSGFGFDLTTIDARVQGADAPESIVTAIERGELLDPDLILI
                     ARGGGARTDLVAFDDERVARAIATCPIPVWVGLGHETDRTVADEVAQRSFKTPTACAA
                     GVIDTVRDAARALDGVFEQILDTVGSALQRGETRLRLASAQVSTSTGTDLRRADRRLL
                     SAAVSITRLAEGSIRTSSERVERSAGLVTRTAPQRLAQRAVQLDAIEAVLRAYDPSAA
                     MARGWSITRTATGALATAANIAVGDRLFITLHDAALTATVDSLEGNET"
     gene            4365..5984
                     /locus_tag="PLP95_00960"
     CDS             4365..5984
                     /locus_tag="PLP95_00960"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HPB44403.1"
                     /translation="MSATSEPASDPILRSSGGFDGGQPTVAADEVRPPRRWMSVRDPS
                     AQARTTVGGVLFWQILANSVGVALVALYVMVLFPSGSEERVRFTALVFVIYVTVIVVV
                     GVPINVFLLGRAVRWVREGVQPTPSQRRLLFRLPLYETLPCVVWWIGGSVIFGVLNKT
                     SSRVSIGIFFAGLVTCAALYLLVESHFRPVFALALRHADLPDGRRDLLPRLMLTWALG
                     SAVPIALLGIAPLLNDNFEARRMPWIAGFSVLGGGAVMVIGTLSVSRPLTRVREALWD
                     VERGQLDIELPIDDLGEIGRLSKGVNDMVAGLRERERLREQFGHQVGRGAFEVAQGSE
                     EALSSVGVLRTVAVLFVDLEGFTRYSERHDPGQVVDMLNRFFEVVVTTVNREGGWVNK
                     FEGDAALCLFGVPDVEEDYASRALIAAARMRSDLQRVRFDPNGIAEERLNAGIAVATG
                     PVIAGFLGTPERYEYTVIGDCVNLASRLCDLAKTVPGKTLVTREAWEQAGRPDGWRMG
                     STLRVRGRRQRVSTMEAQTELRIGRRPIRRS"
     gene            complement(6016..7845)
                     /locus_tag="PLP95_00965"
     CDS             complement(6016..7845)
                     /locus_tag="PLP95_00965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012230045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPB44404.1"
                     /translation="MANPAEGANNRSIDRRLARRVAAYARPQRRRLVGFVTTIVLGAL
                     LGLAPPFLFKAIIDDALPAKDRRQLTVLSLLVVVAAIASAGLSLLERFSSATIGEGLI
                     YDLRRQLYDHVQRLPLGFFTRSQTGSLISRLNNDVIGAQRAFTGTLGTVVSNLVTVAV
                     TLAAMARLEWRLTIASVLLLPLFILPARRIGTRLAAITRSGMQHNAEMNNLMTERFGV
                     SGALLVKTYGDEDRELARFSAQAAHVRDIGVRSAMFTRIFMAVLGLLGAVGTAAIYWW
                     GGRQVIDGAITLGTLTALAALVVRIYEPLTGLTNARVDIMSALVSFERVFEILDAPSS
                     ITDPAEPTALSPVQGRVEFDHVSFRYPTSSPDSIASLEGGFGQHQGEELPVLHDIVAT
                     IEPGQMAAIVGPSGSGKSTLVSLVSRLWDVTEGAVRIDGVDVREVSQRELRASIGVVA
                     QDSHMFHTTVAENLRFAREGATLEELREATAAAQVLEVIERLPDGFDTVVGERGYRLS
                     GGEKQRLAIARMLLKDPAIVVLDEATSHLDAENEHLVQLALARALAGRTALVVAHRLS
                     TIIDADMILVMNEGRIVQRGTHLELLASEGLYRELYTTLNRDD"
     gene            complement(8006..8093)
                     /locus_tag="PLP95_00970"
     tRNA            complement(8006..8093)
                     /locus_tag="PLP95_00970"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8057..8059),aa:Ser,seq:cga)
     gene            complement(8168..8644)
                     /gene="tadA"
                     /locus_tag="PLP95_00975"
     CDS             complement(8168..8644)
                     /gene="tadA"
                     /locus_tag="PLP95_00975"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: protein motif:HMM:NF008113.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HPB44405.1"
                     /translation="MPIDPAVLADPERFMRQALEVAATSIEAGDLPIGALAIHDGEVI
                     ASAHNRRTIDADPLAHAELLAIAQAARNLGEWRLNEVTIVVTLEPCPMCAGAMWAARI
                     GGVVYGAADLKAGAVGSLYNMGSDPRLNHEFDVRPRVLEQECAGILTKFFEARRGA"
     gene            8682..9530
                     /locus_tag="PLP95_00980"
     CDS             8682..9530
                     /locus_tag="PLP95_00980"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HPB44406.1"
                     /translation="MVLATVLAVGSAAIHASWNLLIKTGRDRTLSGWGQFSAAAVLAG
                     LALCVVGFPGRAAVPYLVATATIHIVYVECLVAAYHHGDFSIAYPIARGSGAVLAALA
                     AVVLLGDSMSSLAWASIAVAAGGLISLRWGADLTDASDSLAMVFALGTGVMIAAYSVV
                     DAAGARAVDSGVSYGLASVGAAGMAVTLANLARPSRRRRAGELLVDWRRHLAGGAGTL
                     AAYTMVLVAVRRAPVGYVTMLRESSVVLGALIGWLVLAEPMGSRRVVSSAVVLAGLVA
                     LVVVGA"
     gene            9568..10428
                     /locus_tag="PLP95_00985"
     CDS             9568..10428
                     /locus_tag="PLP95_00985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB44407.1"
                     /translation="MSAGPALDAILVTCDVSPWAEAQRVALLDALEALHCNVEVASWD
                     EQRDWGSAAGIVVVSPFNTAPLHDEFLAWVERCEEQATLVNSAALLNLDSHRRYLFDL
                     EAAGVPIVATRQVAGDAPWQFQRAVLRRFEGTVVMKPAVAASKFGYGIGPADSIDTMA
                     HLEALCLLGDAVIQPYISTVDTVGVAQIVLLRGEVRHAVRKVRDVTVEHTVQPEEREV
                     IAAALDALPEIPVAASVELVWVGDEPALLSFDLVGPNLHAGASGSIVEQYARVLLDTI
                     HRPRIGVVTP"
     gene            complement(10505..10589)
                     /locus_tag="PLP95_00990"
     tRNA            complement(10505..10589)
                     /locus_tag="PLP95_00990"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10553..10555),aa:Ser,seq:gga)
     gene            10714..10812
                     /gene="ffs"
                     /locus_tag="PLP95_00995"
     ncRNA           10714..10812
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="PLP95_00995"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            complement(10867..>11570)
                     /locus_tag="PLP95_01000"
     CDS             complement(10867..>11570)
                     /locus_tag="PLP95_01000"
                     /inference="COORDINATES: protein motif:HMM:NF014566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HPB44408.1"
                     /translation="WPPLTPIRFPAAIVTAVFMVAMFATQLWVAGSADEMGLGTAVLG
                     LVVLVMSVLGMPAVALACAHHWGSRGALRSIGVRVRWIDLPLGVVTAVVMYAAMIVIN
                     LLTLGFGAETGSNLEGVGADGISGGYFLFLLVLAGLLAPITEELLFRGAMLRGLLDKL
                     GTWPSLIVQSVIFGSMHFTPDQGWGNLNLILSLSVLGLCLGIITKATGRLGAAVIAHA
                     IFNSANLLLLWLVAA"
BASE COUNT         1922 a         3543 c         4011 g         2094 t
ORIGIN      
        1 gcacccgggc aaactccgga tataccggaa agccgaagag gcacagcacc cggttgtgaa
       61 acgtgttgac gagttgctga cgagcgttgt cgagtacatc gcgttggctg agcgaggcgg
      121 gaagcgtgcg ggccaccgag ttcaacatgg tgagcagatc gtttgcctcg gagagggcga
      181 gcatctgacc ggacatcgcc aggcgacgct catgggattc gatgagtcgg ctgcgaacga
      241 atgcgctgcc ggcgacgacg aggaccgcag cgatcgccat gaccaggaat tcctggagga
      301 atgcgtcgcc gagatcgccg ccgaatgcca tcgtcgacat caaggtgacc gtccagccga
      361 ggcaccaccc gagtgctccg aggcggtatc cccatccgag tgcggggacg gccaaggcga
      421 cgaggaaaca gaaaatgaac ggcgatgtcg gccccgcact gatccccacc cccaggccga
      481 agatcgcgac gtcgatcagt gcgacgatct gagcgccccg atcgggggca ccgagctgag
      541 tcggaatgat cgagcgccag ctcgtcagga acagacaaac ggtcaacgac accacgacgc
      601 tgtaggagcc gccgaatgcc tgttgggcgc cgaaaatcat gccgtacgcg atggcggccc
      661 accgagccgc ggtaatcgct ggtggaaggg cggcgtgggc ctccggaggg agcttcaagt
      721 ggcgagatgc ggtatcggtg ccaccgaggc gaagcgtcct caacgcggta aatacttctt
      781 cagggtcgta cgctgtcgac gcgctcgaaa caacgccagc ggccgcggcg cggcccatcg
      841 ttttcgggtt gcctgattcg agcatcgagt cctccgcgag ccgacgaggc cgcgagttgc
      901 cacgcgagtc gtcgcgggcg catgtgcatg ctgcaggcgc gagcgatgaa ccgcaataaa
      961 tcgtaccgga attaggacaa ttgcctgtca agcagggtaa aagcctacgg ctcgatgggt
     1021 aggatgactc atccgtaatc gaggcaggtt gaaccgatgg aatccacgac cgattcgatt
     1081 gatgagaagt cgctcggtgt tcttgccggc agcgaagccg cggcggtgca aatcccgggt
     1141 gcccgtcgtc cggatcgccg aacctcactc gacgtagggg gactctcgat ctcgagggtt
     1201 gaatgggggc caccggacgc cccgccactg ctgttcgccc acggggggtt cgattttgcc
     1261 gagacgttga acgtctttgc ccccaagctc gtcgaacgcg gttggcgcgt ggtcagttgg
     1321 gaccaccgtg gacacggtca ttcggagtgg gcacagctct acagtctcga ggccgatatt
     1381 cgagatgcgg cgttcgtcgt ggagtcggtt tcagccgaac gattcccgct cataggccat
     1441 tccaagggcg gggccatgtg cctccagctt gcggtgttgt ttccccacaa attcacccac
     1501 ctggtgaacc ttgacggact tccatcccgc aagacgatgc cagatgtcgc cgaacatcag
     1561 cggattcgaa tggtcgcatc ggagctcgag aattggctgg gccaccgggc ttcactggtc
     1621 gatcgacaac gcccagcggg cacgattgcc gaactggccc agcggcgcgg gcggatgaac
     1681 acgcgggcca caacggaatg gttgcagtat ctggtcacgg tcggggggcg gcgtgatgtc
     1741 gatgggtggc ggtggcgcat cgacccgacg cttcgaatgg gagggttcgg accgtggcgt
     1801 cccgaatggt cgatgtaccg actccccgag gtcacgatgc ccctgttggc gatcttgtgc
     1861 ggcgaaccgg aactgatggg atggggcacc cgcgccgaag acgtcgaacc gtaccttccc
     1921 atcggtgccg agttgatgga actcgcgggc atcggacatt tcatgcacat cgaacagccg
     1981 gacctgttgg tacagatcat cggttcgttc atcggagatc cgcaatgatc acccggctgc
     2041 accacggacg agtggaactc gcgctgcacc ggttgcgaga cggcgacacg accaccccgt
     2101 tgctgttgtt gcacccgctg gggtcgcgca cccccgaagt cgctccgaac tggagcgacc
     2161 ggtggcccgg tccggtgttc gggctggact tcaccggcca cggagcctcg accgttcctc
     2221 gaggcggcgg ctactcggcc gaagtgctgc tcgcagacgt cgacaccgcc ttgacccacc
     2281 tcggcgccgt gtcgatcgtc ggtcggggac tgggcggata cgtcggactg cttgcggcgg
     2341 gtgcccgccc gtctggcgtg cgcggagtgg tcatcgatga cggtcccggt ctgaccggag
     2401 ggggaacggt gccctccagc ccatcgtttg tggctccggt attcgggtcc tcgtcgagtc
     2461 cggacccgtt tgcgcttcag gaactgtcgc gagacattcg gccacccgac tacgtgcgga
     2521 atttcgctcg attcgccttg gaaggttcac cggcggccga accgatcatc gtttcagcga
     2581 ggattctgcc ggcatggttg gaagcggtcg cggacgaaca cggcgtcgtt cgatgttcgt
     2641 tcgaggccgc ggtcgaacgt ctcggtcgct gagcaacccg cgtttgcgaa ccggattgtt
     2701 gttcgcacgg atttcagtcg tcggtctcga gatccgcaac gatctcagtc acccgtagcc
     2761 gcgcccgttc gaggcgttcc cggcacagcg agacgagttg tgatgctcgt tcgacccggt
     2821 cggccaagat gtcgatgtcg acgtctgggc ggtcgagttc cgcgaggatc gcttcgagtt
     2881 cggccattgc gtcgacgtaa ccgatggatt catcggagga acccgatgag gggggcgatg
     2941 ttgtggtcat gtttcgtttc cttcgaggga gtcgaccgtt gcggtcagcg ctgcgtcgtg
     3001 cagcgtgatg aagaggcggt cgccgaccgc aatgttggcg gccgttgcga gcgcacccgt
     3061 cgcggttcgc gtgatgctcc agccacgcgc catggccgcg gacgggtcgt aggcgcgcaa
     3121 caccgcctcg atcgcgtcga gttggaccgc ccgttgcgcg agtcgttggg gcgctgttcg
     3181 ggtgacaagg cccgccgagc gttcgacgcg ttcggatgag gtccggatag atccctcggc
     3241 gagtcgggtg atggagaccg cggcgctcaa cagacgtcgg tcggcgcgtc gcagatcggt
     3301 tccggttgac gtcgagacct gggcgcttgc gagccgcaac cttgtttcgc ctcgttgaag
     3361 ggccgacccg acggtatcga ggatctgctc gaagacaccg tcgagcgctc gagcagcgtc
     3421 gcgaaccgtg tcgatgaccc ccgcggcgca cgcggttggt gtcttgaatg accgttgggc
     3481 gacctcatcg gcgacggtcc ggtcggtctc gtgaccgagg cccacccaaa ccgggatcgg
     3541 gcaggtggcg atcgcccgag caacccgctc gtcgtcgaag gccaccaggt cggtccgagc
     3601 cccaccgcca cgagcgatca ggatcagatc ggggtcgagc agttcgccgc gttcgatcgc
     3661 cgtcacgatg gattcgggcg cgtcggcacc ttggacgcga gcgtcgatcg tcgtcagatc
     3721 gaacccgaac cccgactgac gtaactcatc gagcgcatcg gcgtgcgcgg cgctgccgat
     3781 actcgtgatg atcacgaccc gaagcggcag cggcggtgtt tcgaggcgct gttgggcttc
     3841 gagtagaccc tcgtcttgga gcgctgcgag caagcgggcg cggtctgcgg cgagcgatcc
     3901 gagcgtgaag accgggtcga tgccgctcat gcgcagttgc agttggctgc gtgcggagta
     3961 cacctccacg gtgccgcgta tgcgaacgcg aaccccatcg cccatgcgga cggatccgcc
     4021 gctgcgtttc aggtggcgat tcacgttgac gcggtgccat tcgaacaggg tgacggcgag
     4081 ggtgtggggt gagcgttgcc gctcatcgag atcgcgttcg atcagtgtga agtagacgtg
     4141 gccggccgca gagcgttgaa ggttggaaat ctccccttca acccagactt ctccaccgag
     4201 tgagttcgcc acgacggcgt tcacccaatc gatgagttgt ggaattttcc acgtggtggt
     4261 ctcggtcacg ccggtgaggc taccgatgac ctccgacggt ctcgtgcatg tcccgagcgt
     4321 ctcgttggtt actgtggggt cacttcggag gttgcggggg cgcgatgtcg gcaacgagtg
     4381 aacctgcatc ggacccgatc ctgcgttcgt caggcggatt cgacggtggc cagccgacgg
     4441 tggccgccga cgaggtgcgc ccgcctcgtc gctggatgtc ggttcgcgac ccttcggcgc
     4501 aggcgcgcac aaccgtcggc ggagtgctgt tctggcagat cttggcgaac agcgtcgggg
     4561 tcgcgctggt cgcgctgtac gtgatggtgc tctttccgtc gggatccgag gagcgggtcc
     4621 gtttcacggc gctggtgttc gtgatctatg tgaccgtcat cgtggtggtc ggggtgccga
     4681 tcaacgtgtt cctgctcggc cgcgccgttc gctgggttcg tgaaggggtg caaccaactc
     4741 cgagccagcg gcgcttgctg tttcgcctcc ccctgtacga gaccctcccg tgtgtcgtgt
     4801 ggtggatcgg cggttcggtc atcttcggag tcctcaacaa gacgtcgtcg cgcgtgtcga
     4861 tcgggatctt cttcgccggt ttggtgacct gtgccgcgct gtacctcctc gtcgaatccc
     4921 atttccgacc cgtgttcgcc ctcgctctac gccacgccga cctgcccgac ggccgccgag
     4981 atctgctgcc gcgcctgatg ctgacgtggg cgctgggaag cgcggtgccg atcgccctgt
     5041 tggggattgc tccgctcctg aacgacaact tcgaggcgcg ccgcatgccg tggattgcgg
     5101 ggttctcggt cctcggaggc ggtgcggtca tggtcatcgg gacgctgtcg gtatcgcgtc
     5161 cgctcacgcg ggtgcgcgaa gcgctgtggg acgtggaacg cgggcaactc gacatcgaat
     5221 tgcccatcga cgacctcggt gagatcggac ggctctccaa gggagtcaac gacatggtcg
     5281 cagggctgcg cgaacgcgaa cggctccgcg agcagttcgg ccatcaggtc ggccgggggg
     5341 cctttgaggt ggcccaagga tctgaggagg cgctgagctc ggtgggggtg ttacgaacgg
     5401 ttgcggtcct gttcgtcgac ctcgagggct tcacgcgata cagcgaacgc cacgacccgg
     5461 gtcaggtcgt cgacatgttg aatcgtttct tcgaggtcgt ggtgaccacg gtcaaccggg
     5521 agggcgggtg ggtgaacaag ttcgaagggg acgcagcgct gtgcctcttc ggggtacccg
     5581 atgtcgagga ggactacgcc tcgcgcgcgc tgattgcggc ggcgaggatg cgcagcgacc
     5641 tccagcgggt gcggttcgac ccgaacggta tcgccgagga acgactgaat gcggggattg
     5701 cggtggcgac cgggcccgtg atcgccgggt ttctcgggac tcccgagcgg tacgagtaca
     5761 cggtgatcgg cgactgtgtg aacctcgcgt cacgcctgtg tgacctcgcc aagaccgttc
     5821 caggtaagac gctcgtgacc cgcgaggcgt gggagcaggc cggccgaccg gatggctggc
     5881 ggatggggtc gacgcttcgg gttcgcggtc gccgccagcg ggtgtcgacg atggaggcgc
     5941 agaccgagtt gcgaatcgga cgtcggccga tccgtcgctc gtaagcgggg tgggttgacc
     6001 gtgagcgcgc ggcgctcagt cgtcgcggtt gagcgtcgtg tacaactcgc ggtaaaggcc
     6061 ctcggacgcg aggagttcga gatgcgtgcc gcgttgaacg attcgccctt cgttcatcac
     6121 caggatcatg tcggcatcga tgatggtgga cagtcggtga gcgacgacga gtgcggtgcg
     6181 cccggcaagt gcgcgggcca gcgccaactg cacgaggtgt tcgttctcgg cgtcgaggtg
     6241 gctcgtcgcc tcgtcgagca cgacgatggc cggatccttc aacaacatcc gagcaatcgc
     6301 gaggcgttgc ttctcgccgc cgctgagccg atagccgcgt tcgcctacga cggtgtcgaa
     6361 cccgtccggc agccgttcga tcacctcgag aacctgagcc gcagcggtcg cctcgcgcag
     6421 ttcctccagg gtggcaccct cgcgtgcgaa tcgcaggttc tcggccaccg tcgtatggaa
     6481 catgtgggaa tcctgcgcaa ccacgccgat cgatgcccgc agctcgcgtt gtgacacctc
     6541 gcgaacatcg acgccgtcga ttcgaacggc gccctcggtg acatcccaca agcgactgac
     6601 caatgagacg agcgtcgact tccccgaacc cgacggtccg acgatcgccg ccatctgtcc
     6661 cggctcgatg gtggcgacga tgtcgtgcaa cacggggagc tcctcgccct ggtgttgtcc
     6721 gaagccgcct tcgagcgagg cgatcgagtc ggggctcgac gtcgggtatc gaaacgagac
     6781 gtgatcgaac tcgacgcgtc cctgcaccgg tgacaatgcg gttggctcgg ccgggtcggt
     6841 gatcgacgaa ggtgcgtcga gaatctcaaa gacgcgctcg aagctcacca acgcgctcat
     6901 gatgtcgact ctggcattgg tgagtccggt gagcggttcg tagattcgga caacgagcgc
     6961 tgcgagagcg gtcagggtgc ccagggtgat cgcgccgtcg atcacctgcc gtccgcccca
     7021 ccagtagatg gcggcggtac cgaccgcgcc gagcagcccg agcaccgcca tgaagattcg
     7081 ggtgaacatg gcagaacgca cgccgatgtc tcgcacatgg gcggcctggg cggaaaagcg
     7141 cgcgagctcg cggtcctcgt cgccgtaggt cttgaccagc aatgcccccg agacgccgaa
     7201 ccgctcggtc atgaggttgt tcatctcggc gttgtgttgc atacccgatc gggtgatggc
     7261 ggcgaggcgt gtgccgattc gccgggcggg caggatgaac agcggcagca gcagcaccga
     7321 cgcgatggtg agtcgccatt cgagacgggc catcgcggcg agcgtcacgg cgacggtgac
     7381 gaggttcgac acgacggtgc cgagcgtgcc ggtgaaggcg cgctgagctc cgatgacgtc
     7441 gttgttcaag cgactgatga gcgatccggt ttgggagcgg gtgaaaaacc cgagcggaag
     7501 gcgttggacg tggtcgtaga gctgacgacg caggtcatag atgagccctt cgccgatcgt
     7561 ggccgacgag aaccgttcca gcaggctgag ccccgcggag gcgatggccg cgaccaccac
     7621 gaggagcgac agcacggtga gctgtcggcg gtccttggct gggagcgcgt cgtcgatgat
     7681 ggccttgaac agaaatgggg gtgcaagccc caggagggct ccgagcacga tcgtggtgac
     7741 gaaccctacg agtcggcggc gctgcggacg cgcataggcg gcgacgcgac gcgccaagcg
     7801 gcggtcgatc gaacggttgt tcgctccctc ggcagggttg gccatggagt gtaggacatg
     7861 cacaggtggg ctccagcggg aggggtggtg tgctccgcgg cgagcggcaa cacaacgtgg
     7921 cttgatcggt ttccccgctg accccgacgt tgcggaggcg aaccggcccc gatgcggggc
     7981 tgactcgcgc tccaagccgt tcgtctggcg gagagtgggg gattcgaacc cccggaggcc
     8041 gtaacctcaa cggttttcga gaccgtccca ttcgtccgct ctggcaactc tccgccgaca
     8101 aagatagcgg aacgtcaccg gggccgtgcc agtcgaccga tgccggagcc ctcgggcgac
     8161 cgtgtggtca ggcgccgcga cgggcctcga agaacttggt gaggatcccg gcacactcct
     8221 gttcgaggac gcggggccgt acgtcgaatt cgtggttcag cctgggatcg ctgcccatgt
     8281 tgtacaacga gccgaccgca ccggctttga ggtcggctgc gccgtacacg acgccgccga
     8341 tccgtgcagc ccacatcgct cccgcgcaca tcgggcaggg ttcgagggtg acgacgatcg
     8401 tcacctcgtt gaggcgccat tcgccgaggt ttcgagcggc ctgcgcgatg gcgagcagtt
     8461 cggcgtgggc cagcggatcc gcatcgatcg tgcgacggtt gtgggcgctc gcgatcacct
     8521 caccgtcgtg gatggcgagc gcgccgatgg gaagatcccc cgcctcgatc gacgtcgcag
     8581 ctacctcaag ggcctggcgc atgaaacgtt cggggtcggc gagtactgcg gggtcgatgg
     8641 gcatgaggcc gatcgtaggt gactgatagc gtgcgcccga gatggtgctc gcgaccgtgc
     8701 tcgctgtggg atccgccgcg atccatgcgt cgtggaacct gctcatcaag accggacgtg
     8761 accgcacact gtcgggttgg ggacagttca gcgccgcggc ggtccttgca gggttggcgc
     8821 tgtgtgtcgt cggattcccg gggcgtgccg ccgtgccgta tctcgttgcc acagccacga
     8881 ttcacatcgt gtacgtcgag tgtctggtgg cggcgtacca ccacggtgat ttctccatcg
     8941 cgtatccgat cgcccggggc agtggcgctg tacttgcagc gctggcggcg gtagttctac
     9001 tcggagactc gatgtcctcg ttggcgtggg cgtcgatcgc agtggcggcc ggcgggctga
     9061 tttctctgcg ctggggtgct gacctcaccg atgcttccga ctcgctggcg atggtgttcg
     9121 cgcttggcac cggcgtgatg atcgccgcct actccgtggt cgacgccgca ggggcgcggg
     9181 cggtcgactc gggtgtctcc tacgggttgg catcggtcgg tgccgcgggt atggcggtga
     9241 cgctggccaa cctcgcccgg cccagtcgcc gtcgtcgagc gggtgaattg cttgtcgatt
     9301 ggcgccgaca cctcgccgga ggtgcgggca cgttggcggc gtacacgatg gtgctggtgg
     9361 cggtgcggcg ggcgccggtc gggtacgtga ccatgttgcg cgagtcctcg gtggtgctcg
     9421 gcgcgctcat cggctggttg gtgcttgccg agccgatggg atcgcgccgt gtcgtgtcat
     9481 ccgccgtcgt gcttgcgggc ctggtcgcgc tcgtcgttgt cggtgcctga ttcgaccggg
     9541 tcgctcgccg tgacaaactg gcgtcgaatg tccgctggcc ccgcactcga cgccattctc
     9601 gtgacctgtg acgtttcgcc atgggccgag gcccaacggg tagccctgct cgacgcgctc
     9661 gaggcgcttc actgcaatgt ggaggtcgcc tcgtgggacg agcagcggga ctggggttcg
     9721 gcggcgggca tcgtggtggt cagtccgttc aacaccgcgc cgctccacga tgaattcctc
     9781 gcgtgggtcg agcggtgcga ggaacaggcc acgctggtca attcggcggc gttgttgaac
     9841 ttggactcac atcgacggta cctgttcgat ctcgaggcgg cgggtgtgcc gatcgtggcg
     9901 acgcgtcagg tggcggggga tgctccgtgg cagttccaac gggcggtgtt gcgccgtttc
     9961 gaagggaccg tcgtgatgaa gcccgcagtt gcggcatcga agttcggcta tgggatcggt
    10021 cccgcagaca gcatcgacac gatggcgcac ctcgaggcgc tctgtcttct cggcgatgcc
    10081 gtgattcagc cgtacatctc gaccgtcgat acggtgggcg tcgcgcagat cgtgttactt
    10141 cgcggcgagg tccgtcatgc ggtgcgaaag gtccgcgacg tcacggtgga gcacacggtg
    10201 caacccgagg agcgtgaggt catcgcggcg gcactcgacg ccttgccgga gattccggtc
    10261 gcggcgtcgg tggaactcgt ctgggtcggc gacgaaccgg cgctgctgtc gttcgatctc
    10321 gtcgggccga acctgcacgc cggcgcttcc ggctcgatcg tggagcagta cgcccgcgtt
    10381 ctactcgaca ccatccatcg cccccgcatc ggggtcgtga cgccctgacc ttcccgcccc
    10441 actcgttctc cgataaccag tggtcgtgat cacgaccact ggttatcgga gaacggtcac
    10501 acagcggaga cggtgggatt cgaacccacg gtgagttgcc ccacacacgc gttccaggcg
    10561 tgccgaatcg tccgctctcg cacgtctcca atgaatgtcg gcaggcatcg tagcggctca
    10621 gtcacgtcgg agcgaaggcg gtgccgactc acacgagatg gcaccggtgt cgggctccgc
    10681 tacacttgcc accggtccgt gctctggtga gacggtcggg tcctgtgcag cgccggtccg
    10741 tgaaccgggt cagggtcgga aggcagcagc cctcagcgat ttccggtggg tgccgcagat
    10801 cgcctgaccg tctcaccagg gtccgggccg atgcattttt cggtcatcga tgcttcgtgc
    10861 gcaccgtcag gcggcaacga gccacagcag caacaggttc gccgagttga agatggcgtg
    10921 cgcgatgacc gccgcgccga gccgtccggt cgccttggtg atgatgccca gacacaggcc
    10981 caggacggag agcgacagaa tcagattgag gttgccccaa ccctgatccg gggtgaaatg
    11041 catcgagccg aagatcaccg attggacgat gagtgacggc caggttccca gcttgtcgag
    11101 aagcccgcga agcatggcgc cgcgaaacag cagttcctcg gtgatcggag cgagtagacc
    11161 cgccaggacc aagaggaaca ggaagtaacc accggagatg ccgtcggccc cgaccccctc
    11221 gaggttggac ccggtttcgg caccgaaacc cagcgtcaac aggttgatca cgatcatcgc
    11281 tgcgtacatg acgaccgccg tgaccacacc gagcgggagg tcgatccacc gcacccttac
    11341 cccgatcgat cgcagcgctc cgcgcgaacc ccagtggtga gcacacgcca gggcgaccgc
    11401 cggcataccc aacaccgaca tcaccagcac cacgagcccg agaaccgcag ttcccagacc
    11461 catctcgtcg gcggaaccgg cgacccacag ctgggtggcg aacatcgcga ccatgaagac
    11521 cgcagtgacg atcgccgcgg ggaaccggat cggtgtcagt ggcggccacc
//
LOCUS       DAOOMA010000016        11369 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_6393_length_11369_cov_3.497172, whole genome shotgun sequence.
ACCESSION   DAOOMA010000016 DAOOMA010000000
VERSION     DAOOMA010000016.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 11369)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11369)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11369
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6393_length_11369_cov_3.497172"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1338
                     /locus_tag="PLP95_01005"
     CDS             <1..1338
                     /locus_tag="PLP95_01005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB44409.1"
                     /translation="KVVIYTPQADDPVLNYVYAQVGVNDSPDQNFASFEGYAEIFGRY
                     YETYGRTVTLQRYEATGPISDSAAAMADAETIARDLAPFAVIGGPNLTNAFADTLARN
                     KVICISCTPLQPAEWYVERAPYVWDVMPNATQALDMVAEYLIKRVAGRPAEFAGDEAL
                     HRRDRVLGLVRVDSSESSSQLEAYFVDTLRAGGVELATIQTYESPTDLAATGKDIITA
                     LKEAGVTSVIFSGDPLAPQALTQIATQQEYFPEWILGASSLVDTSLFSQTYDQTQWAH
                     AFGVSPLFVPVRPGTAGAAFLYEWFFGEPAPSQSVALITGPLQVLFGAIQGIGPNVEP
                     QAFEDVLFSGRVYDSSPVNPQVSFGDRGFFTVTDYSALDDMVEMWWDPNATEVNELGR
                     EAKGHWRYVDGGARTLPGQWSPGRSALFDPAGTVMSYDRAPAGTEMPDYEPLR"
     gene            complement(1416..2279)
                     /locus_tag="PLP95_01010"
     CDS             complement(1416..2279)
                     /locus_tag="PLP95_01010"
                     /inference="COORDINATES: protein motif:HMM:NF021287.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043248 - proteasome assembly [Evidence
                     IEA];
                     GO_process: GO:0051131 - chaperone-mediated protein
                     complex assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAC2 family protein"
                     /protein_id="HPB44410.1"
                     /translation="MTELVELHSHPQLSNPTLIVALEGWIDAGNAAAAAMTSILDNTD
                     TELVATFDSDELLDQRARRPIMSIVDGHMNELTWPATELRAGIDESGNHVLLLIGAEP
                     DHRWGAFCQEVVSLALDLEVQQIIGLGAYPAPVPHTRTTMLSLTSPSSSIVEAHHGFV
                     SGTVEVPAGVQAAIEMHAHDAGISALALWAQVPHYISAMTYSAGSLALIEGLARVGGL
                     TFPTAELAIEAAATRERLDELVAGNPQHEAMLRQLEEAADSMPLTNQLGPLPSGDEIA
                     DEFERFLREQG"
     gene            complement(2287..3336)
                     /locus_tag="PLP95_01015"
     CDS             complement(2287..3336)
                     /locus_tag="PLP95_01015"
                     /inference="COORDINATES: protein motif:HMM:NF013775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HPB44411.1"
                     /translation="MTNETAPSGIHIDRVTEWMSARIPQLAAPLQFSLITGGLSNLTY
                     SVADRDGRRWVLRRPPLGQVLATAHDMGREFRIMNALQSSAVPVPSLVGFESDPSVND
                     APFYVMDFVDGTVVRDAEVAQRLGTVERRRASESLVDVLAAIHEVDVEAVGLGDLGRH
                     DGYLERQLKRWYQQYQGSKEQNPAVDLPIVDQVHDLLAARIPPQQGTSIVHGDYRLDN
                     CMIGADGSVVAVLDWEICTLGDPLADLGLLCVYWTDPGSTPALPQASPTALDGFMTTA
                     EVCRRYAEVSGRDLSHIDTYIAFGYWKLTCIMAGVWSRYAAGALGETDQHQADGFANM
                     VTSLAALTAEAAEKV"
     gene            3436..4140
                     /locus_tag="PLP95_01020"
     CDS             3436..4140
                     /locus_tag="PLP95_01020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020378213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPB44412.1"
                     /translation="MVEVTDATFSDLVLARSMQVPVVVDLWASWCQPCKTLGPILEAV
                     IAETDGAVEFAKVDVDANPQVANAFQVKSIPAVFAMDQQQIVDSFLGAQSEAFVREFV
                     AKLVPTAEQNEVERLYSLGDEASLRAALELEPTNERVVVALAELLVIGGRGEEAEALL
                     AAIPEDAEVRRIRAQIRTGQIDAAGIDVELELAQLLDRVKTDDAARQRYIDLLELLGD
                     DERVGDFRRKLTARLY"
     gene            4181..4501
                     /locus_tag="PLP95_01025"
     CDS             4181..4501
                     /locus_tag="PLP95_01025"
                     /inference="COORDINATES: protein motif:HMM:NF025078.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4157 domain-containing protein"
                     /protein_id="HPB44413.1"
                     /translation="MIRRESGEGYTVWLGAPVPRGATAITFGRHILMRPRGSGNEHLL
                     RHELIHVRQWQEHGVVRFAVRYLGAYLRGRWRGYGHWDAYRRIPLEIEAEWGAQRMSS
                     TGPR"
     gene            4583..7435
                     /locus_tag="PLP95_01030"
     CDS             4583..7435
                     /locus_tag="PLP95_01030"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HPB44414.1"
                     /translation="MAFPLRLPAPVGDRDVPKAYGVAVEIVAALAGIGAIAALSTNTF
                     ALIIVGAALVVVAGFALVFSRPAIVVEISIITMWFDSVGVGPLRTGRLVAGLTVVVLA
                     ARIMSSPWRPPMLAPRAWAWPAAFFIWAFASGFWAAETGTWLVGISELTLGFIYAIIL
                     LAFVEDEAHFARSMKAWVWSGVPIAIISYFFYNKVEEVQEEYGLENRIVGFSGNANHY
                     ARLLILAVAVAFLFSRRAQSRRARLGYLVAAFLFSVALVTTGSRAGMVAMVAMMLYLA
                     LTLPGLPKRQRVRSMFGGGALVIVGWLLAAWWNPDRYSIGGFLGDAGAGRIDLWNAAI
                     HSIQQRPINGHSISAFRTRMLDMLLTAPGGSLDVTNQLRNRQAGGLEAHNTYLTIILD
                     LGFIGLALYLGVIASVVRNLWDMRKTQLADWAWILIGAQVVLSLTAAFGSTYNVKFQW
                     MDVGVAGAVYVRARSTDRVDRVRSHSGLGSLRNLVSPRHSGIERPYAGERAFAAPMDL
                     RLRYPLRWVMVGAVVAGAVLAGGITAMFGSPTYSSRARVIVADFANAQPGEPLTASND
                     GIQFVLNLTRSSVYYAEVLRQSGVDLTVDEVAQSVVPTRPNFGANVAITATTKDEQTT
                     RRIGSVLVSSLDAVVEAARSGSVVITDVWTLRLSRDLPADYDGPLYLVPYRTPVETVT
                     TPRVVTHALTGAALAALLVFVWALAAHSRQRVRSDEDLERQLGLTMVAAVPRPLLARE
                     RHIEEHYRTAADLIDSACPVDPRIVGFASTGIDGLRSRVALWCALGLSNLSSRPIVIV
                     DLDATSANLSRQLGARRAQGIVDVARGGVDLASVTRPIARRMLPRSFARLVASAAAPV
                     SLVPVGGRGVSSDEVSMLSTTIAELVDQLAEDALVIVSLPSVPGPVTVEPTLVHCDAV
                     LMTVLDGWTPIDDAILTIDSLVAAADDRVGLLLVDQ"
     gene            complement(7457..8638)
                     /locus_tag="PLP95_01035"
     CDS             complement(7457..8638)
                     /locus_tag="PLP95_01035"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HPB44415.1"
                     /translation="MVVSNTAQVHDDGAALPRITSLDGVRGVAILLTLVFHSFDSRLS
                     WAVFGERGVDLFFVLSGFLITGILIETRDHPRYFRNFIARRAVRIFPLYYLFLVLMLF
                     VAPVIVNHLPSFVTVPEEFFELQNGPDGLWTVWVYLQNFVLGRGPHQLPGLGHLWSLA
                     VEEQFYLFWPVVMYFVPAPSRLRWFCIGILGSMTLRTALLWGGVINTYGAREYTFNRL
                     DTLLIGACGALYLRDPKAREFIGTILGWVARPIVMAPILLIGLMPIWTVSDVFPNFAY
                     GPGLTLLALVFLLYVVRAAQGQLGPRVERATNWPFNLVLGKYSYAMYLFHFPIAHTMQ
                     STMSKLGDRLGPIGQLVGSAVSFGVTLGASLLIARITWVLWEEPWLRFKKRFAYRPIE
                     T"
     gene            8865..9680
                     /locus_tag="PLP95_01040"
     CDS             8865..9680
                     /locus_tag="PLP95_01040"
                     /inference="COORDINATES: protein motif:HMM:NF024245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HPB44416.1"
                     /translation="MDPTDSLDDILRPQRFSERVRARWATPSADRAPSASVRLVAVAV
                     AVVVGVATVVAIVARPPQSVGVATLPQASVPDVGGERAPSDASSGAVGEGAGGAGAGG
                     AAGAEAGIAGQGAAGAGEWVVHVAGAVLEPGLVTLSPGSRVGDAIAAAGGPAAVADLD
                     AINLAEVLADGVRVRVPAVGETPPANASALDGSGAARNGAGSASGGDSGLININTASV
                     EELQQLPGVGPSTAEAIVAHRDKHGPFSSPDALDDVRGIGPAKLSALLDLVAT"
     gene            9677..>11369
                     /locus_tag="PLP95_01045"
     CDS             9677..>11369
                     /locus_tag="PLP95_01045"
                     /inference="COORDINATES: protein motif:HMM:NF015714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HPB44417.1"
                     /translation="MSERQLFVAAAAVVLGARYAVAVPFAVVMMAVVGACVASASRPR
                     SDAGVRFVAAVAIAAFAISASFLAARAEAGVSIIDTSHEVSGVATLVSDPQQRFGSSV
                     VEMRLDARRYRAIIPGELAPTIADARMGQRVRVRGTAAAIDGPTGWLKSRHFAGQLRL
                     RTLERVDSGSWLWRSANGLQALVAAGQRPLAADERALFLGVVFGDDRGQTAVQQYRFR
                     TAGTAHLMAVSGQNVVLLVALAMPLLERLARHARFVSVLALIGWFAVLTRAEPSVLRA
                     TVMVGLGALVSWQGRYASGLRVLCVAVIALVLADPLLVWSLGFRLSVGASLALITLSR
                     PLIEVLVGPRWFASALGVSLAAQAGTAPMIVSTFGPVGLLSPLVNLVAVPLGGALMVW
                     GSVSGPIAGLLGRPVDTFAAIVSRGLIWGLEASAAIGAWSRLPKLGVIGTLAMSALCV
                     WPWLRTLHVRLSTARLVAWWLVAVVIVDLVVGEVRAPQPQVRSAVGSATLWSVGRTNV
                     MVFDGPVRDGAALDLLAGMRRPVLEAVIVVGGGKVSAGAVWALRQVTTVGVVFAQD"
BASE COUNT         1717 a         3600 c         3976 g         2076 t
ORIGIN      
        1 aaggtggtga tttacacgcc ccaggccgac gacccggtac tcaactacgt ctacgcgcag
       61 gttggggtga acgactcgcc cgatcagaat ttcgccagct tcgaggggta cgccgagatc
      121 ttcgggcggt actacgaaac ctacgggcgc acggtgacgt tgcagcgtta tgaggccacc
      181 gggccgatca gcgactcggc cgcggcgatg gccgacgccg agacgatcgc ccgtgatctc
      241 gcaccattcg ccgtcatcgg tggtccgaat ctgaccaacg ccttcgccga cacgctggct
      301 cgcaacaagg tgatctgtat ctcgtgcacg ccgctgcagc cggccgagtg gtatgtggaa
      361 cgcgccccgt acgtctggga cgtgatgcca aatgccaccc aggccctcga catggtggcc
      421 gaatacctca tcaaacgcgt cgccggcaga cccgcagaat tcgccggcga cgaggcactg
      481 caccgacgcg atcgcgtcct cggtctcgtc cgcgtcgatt cgagcgagtc gtcgtcgcaa
      541 ctcgaggcct acttcgtcga cacccttcgg gccggagggg tcgagttggc gacgattcaa
      601 acctatgagt cgcccacgga tctggccgcg accggcaagg acatcatcac cgcgctcaag
      661 gaggcggggg tcacctcggt gatcttcagc ggcgatccgc tcgccccgca ggcgctcacc
      721 cagatcgcca cccaacagga gtacttcccg gagtggattc tgggtgcctc cagccttgtc
      781 gacacgtcgc tgttctccca gacctacgac cagacccagt gggcacatgc attcggcgtg
      841 agcccactgt tcgtgccggt ccggccgggt accgccggtg cggcgttctt gtacgagtgg
      901 ttcttcggcg aacccgcccc gtcacagtcg gttgcgctca tcaccggtcc gctgcaggtg
      961 ctgttcggcg caattcaagg catcggaccc aacgtcgagc cgcaggcctt cgaggacgtg
     1021 ctgttctccg gtcgggtcta cgacagctct ccggtgaacc cccaggtgtc gttcggcgac
     1081 cgtggattct tcaccgtcac cgactactca gcgctcgacg acatggtgga gatgtggtgg
     1141 gacccgaacg ccaccgaggt gaacgagttg gggcgcgagg ccaaaggaca ttggcgttat
     1201 gtcgacggtg gcgctcgtac cctgccgggg caatggagcc cggggcgctc ggcgctgttc
     1261 gatcctgcag gcaccgtgat gagctatgac cgggcgcccg ccggaaccga gatgcccgac
     1321 tacgaaccgc tccgctagcc ctgctcccgc aggcagtttt gttatccctg ctcccgcagg
     1381 cagttttgct agccctgctc ccgcaggcag ttttgttatc cctgctcccg caggaagcgt
     1441 tcaaattcgt cggcgatttc gtcgccggag gggagggggc cgagttggtt ggtcagcggc
     1501 atcgaatcgg ccgcctcctc aagctggcgc agcatcgcct cgtgctgggg gttccctgcg
     1561 acgagttcgt cgagccgctc gcgggtcgcc gcggcctcga tggcgagttc cgccgtcgga
     1621 aacgtgaggc cgccgacccg ggcgaggccc tcgatcagtg ccaacgaacc ggccgaatag
     1681 gtcatcgcac tgatgtaatg cggcacctgg gcccacaacg ccagggcgga aattccggca
     1741 tcgtgggcgt gcatctcgat ggcggcctgg acgcctgccg gaacctcgac cgttccgctg
     1801 acgaaaccgt ggtgggcctc gacgatcgat gaggacggcg acgtcaacga caacatcgtc
     1861 gtgcgggtgt ggggaaccgg ggcggggtag gcaccgaggc cgatgatctg ctgaacctcg
     1921 agatcgaggg ccagcgacac gacctcctgg cagaacgcgc cccagcggtg atcgggttcg
     1981 gccccgatca acaacaagac gtggttgccc gactcgtcga tgccagctcg caactcggtg
     2041 gccggccagg tcagttcgtt catgtgcccg tcgacgatcg acatgatcgg gcgtcgcgcc
     2101 cgctggtcga gcaattcgtc ggaatcgaag gtggcgacga gttcggtgtc ggtgttgtcg
     2161 aggatggagg tcatcgccgc ggcagcggcg ttgccggcat cgatccaccc ttcgagcgcg
     2221 acgatgagcg tcgggttgga cagctgcggg tgggaatgga gttcgacgag ttcggtcatg
     2281 aggggatcac actttctcgg cagcctctgc ggtgagcgcc gcaaggcttg tcaccatgtt
     2341 cgcgaacccg tcggcctgat gctgatcggt ttcgcccaga gcacccgccg cgtaccgcga
     2401 ccacaccccg gccatgatgc aggtgagctt ccagtatccg aacgcgatgt aggtgtcgat
     2461 gtggctcagg tctcgacccg agacctccgc gtatcgccgg cacacctcgg cggtggtcat
     2521 gaacccgtcg agcgcggtcg gcgacgcctg gggaagcgct ggcgtcgaac cggggtcggt
     2581 ccagtacaca cacagcagtc cgaggtcggc gagcggatct ccgagcgtgc agatctccca
     2641 gtcgagcacg gcgacgaccg aaccatcggc gccgatcatg cagttgtcga gccggtagtc
     2701 gccatgcacg atcgaggtgc cctgctgcgg cggaatgcgc gccgccaaca ggtcgtgtac
     2761 ctgatcgacg atcggcaggt cgacggccgg gttctgttcc ttcgacccct ggtattgctg
     2821 ataccagcgc ttgagttgtc gttcgagata gccgtcgtgg cggccgagat cgccgagccc
     2881 gaccgcctcg acgtcgacct cgtggatggc cgcgagcaca tcgaccagag actccgaggc
     2941 ccgccgtcgt tcgacggttc cgagccgttg ggcgacctcg gcgtctcgca cgaccgttcc
     3001 gtcgacgaag tccatcacgt agaacggggc gtcgttgacc gacgggtcgg attcgaaccc
     3061 caccagactc ggcaccggaa ccgcgctgga ttgcagcgcg ttcatgatgc ggaactctct
     3121 gcccatgtcg tgcgcggtgg cgagcacctg gccgagcggt ggccgtcgca gtacccagcg
     3181 gcggccgtca cggtcggcga cggagtaggt gaggttggac aggccaccgg tgatgaggga
     3241 gaactgcagc ggtgccgcga gctgtggaat ccgagcgctc atccactcgg tgacccggtc
     3301 gatgtggatg ccgctggggg ccgtctcgtt cgtcaccgaa gtccctttca tggccgattc
     3361 gtggtcgtca caacctagtg ctgtctcgcc cagggcgcgg ccgaaggggc tcggaccgat
     3421 cgctacgatg accccatggt tgaagtgacc gatgcaacgt tttccgacct cgtgctcgca
     3481 cgctcgatgc aggttcccgt ggtggtcgac ctgtgggctt catggtgtca gccgtgcaag
     3541 acgctcgggc ccatccttga ggcggtgatc gcggagaccg acggcgcggt ggaattcgcc
     3601 aaggtcgacg tcgacgcgaa tccacaggtc gccaacgcgt tccaggtgaa gtcgatcccc
     3661 gcggtgttcg cgatggacca gcaacagatc gtcgattcgt tcctcggcgc ccagtccgaa
     3721 gcgttcgtgc gcgagttcgt ggccaagttg gtgcccaccg ccgagcagaa cgaggtggag
     3781 cggctctact cgctcggcga cgaagcctcg ctgcgagcgg cgctcgaact cgaaccgacg
     3841 aatgagcgcg tcgtggtcgc gttggcggaa ctgctggtga tcgggggtcg gggtgaagag
     3901 gccgaggcgc tgttggcggc aattcccgag gatgcggagg ttcgtcgaat tcgggcgcag
     3961 attcgaacgg ggcagatcga cgcggccggt atcgatgtcg aattggaact cgctcagttg
     4021 ctcgaccgcg tcaagaccga cgatgctgcc cggcagcgtt acatcgacct gttggaactg
     4081 ctcggcgacg acgaacgggt gggcgatttc cgccgaaagc tgacggcgcg gctgtactga
     4141 tggccatcgg cggcaacgcg atctcgactc ggagcctcaa gtgattcgac gcgaatcggg
     4201 cgagggctac acggtgtggc tcggagcgcc ggtaccgcgc ggcgcaaccg ccatcacctt
     4261 cggtcggcac atcctgatgc ggccccgcgg ttccggcaac gaacacctgt tgcgtcacga
     4321 actcatccac gtgcgccagt ggcaggaaca cggcgtcgtc cgtttcgcgg tgcggtacct
     4381 cggcgcgtat ttgcggggtc ggtggcgcgg gtatggccac tgggacgcct atcggcgcat
     4441 tccgctcgaa atcgaggcgg aatggggagc gcaacgcatg tcgtcgaccg gcccgcgatg
     4501 accgtttcaa cgaccgcgac cgctaacgtg gtcaggtcac atcgcaggct cgacggcacc
     4561 gcagagggcg ggtaggggag aaatggcgtt tccattgcga cttccggcgc cggtcggcga
     4621 tcgcgatgtc ccgaaagcct acggcgtcgc cgtcgaaatc gtcgctgcac tcgccgggat
     4681 cggcgccatc gccgcgctca gcaccaacac ctttgcgctc atcatcgtcg gtgctgccct
     4741 cgtcgtcgtc gccgggttcg cgctcgtgtt cagccgcccg gcgatcgtgg tggagatctc
     4801 gatcatcacc atgtggttcg actcggtcgg agtcggtccg cttcgaaccg gccgtctcgt
     4861 cgccgggtta accgtggtgg tgctggccgc gcgcatcatg tcctcgccgt ggcgcccacc
     4921 gatgctcgcg ccgcgcgcgt gggcgtggcc ggcggcgttc ttcatctggg ccttcgccag
     4981 cgggttctgg gcggcggaga cgggaacgtg gctcgtcggc atctccgaac tcacgctcgg
     5041 gttcatctac gcgatcatcc tgttggcgtt cgtcgaggat gaggcccact tcgcccgcag
     5101 catgaaggcg tgggtgtgga gcggagttcc gatcgcgatc atctcgtact tcttttacaa
     5161 caaggtcgag gaggtccagg aggaatacgg cctcgagaac cggatcgtcg gattctcggg
     5221 caacgccaat cactacgcgc gcctgctgat tctcgccgtc gccgtagcgt ttctgttttc
     5281 gcgtcgcgcg caaagccggc gtgcacgcct cggctacctg gtcgccgcgt tcttgttctc
     5341 cgtggcgctg gtcacgaccg gttcgcgagc cggcatggtc gcgatggtcg ccatgatgtt
     5401 gtatctggca ctcaccttgc ccggtctgcc gaaacgccag cgcgtccggt cgatgttcgg
     5461 tggcggggcg ttggtcatcg tcggctggct gttggccgcc tggtggaacc ccgaccggta
     5521 ctcgatcggg ggattcctgg gcgatgcggg tgccgggcga atcgacctgt ggaacgcggc
     5581 gattcactcg atccaacagc gtccgatcaa cggccattcg atcagcgcgt tccgaacgcg
     5641 catgctcgac atgttgttga ccgcgccggg tggttcgctc gacgtcacga atcagctgcg
     5701 taaccggcag gcgggtggcc tggaagcgca caacacctac ctgaccatca tcctcgatct
     5761 cggtttcatc gggttggcgc tctacctcgg cgtgatcgcg tcggtggtgc ggaacctgtg
     5821 ggacatgcgc aagacccagt tggcggattg ggcgtggatt ctgatcgggg ctcaggtcgt
     5881 gctgtcgctg acggcggcct tcgggtcgac ctacaacgtg aagttccagt ggatggacgt
     5941 cggcgttgcc ggagcggtct acgtgcgcgc tcgtagcacc gaccgcgtcg accgggttcg
     6001 gtcccacagc gggctggggt cgctgcgcaa ccttgtctcg cctcggcatt ccgggatcga
     6061 acgtccctac gccggcgagc gggcattcgc cgccccgatg gacctgcggc tgcgttaccc
     6121 cctgcggtgg gtgatggtgg gcgcggtggt ggccggcgcc gtgttggccg gcggaatcac
     6181 ggcgatgttc ggttcgccga cctacagcag ccgggcccgg gtgatcgtcg ccgactttgc
     6241 gaatgcgcag cctggggagc cgctgaccgc gagcaacgac gggattcagt tcgtgctgaa
     6301 cctcacacgc agttcggtct attacgccga ggtgcttcgc caatccgggg tcgacctgac
     6361 cgtcgacgag gtcgcccagt ccgtcgtgcc cacgcgcccg aacttcggag ccaacgtcgc
     6421 gatcacggcg accacgaagg atgagcagac cacgaggcgc atcggttcgg tgttggtgtc
     6481 ctcgttggac gcggtcgtcg aggccgccag gtcggggtcg gtggtgatca ccgacgtgtg
     6541 gaccctgagg ctgtcacgag accttccggc cgattacgac gggccgttgt atctcgttcc
     6601 ctaccgcacc ccggtggaaa cggtgacgac gccccgtgtg gtcacccacg cgctgaccgg
     6661 agcggcgttg gcggcgctgc tggtgttcgt ctgggcgctc gcggcgcatt cccgccaacg
     6721 cgtccggtcc gacgaggacc tcgagcggca actcgggctc acgatggtgg cggcggtgcc
     6781 gcgtccgttg ctggcccgtg aacgccatat cgaggagcat tatcgaaccg ccgccgatct
     6841 catcgattcc gcctgtccgg tcgacccgcg aatcgtcggg tttgcctcga ccggcatcga
     6901 cgggctccgg agccgggtgg cgttgtggtg tgcgttggga ttgtcgaacc tgtcgagtcg
     6961 acccatcgtg atcgtggacc tcgacgcaac ctccgcgaac ctgagccgtc aactgggggc
     7021 ccgtcgtgct cagggcatcg tcgatgtggc ccgcggtggg gtggacctgg cttcggtgac
     7081 ccggccgatc gcacgccgga tgttgccgcg atcgttcgcc cggctcgtgg cgtctgcggc
     7141 cgcgccggtt tcgctggtgc cggtgggagg gcgcggcgtg tcctccgatg aggtctcgat
     7201 gttgtcgacg accattgcgg aactcgtcga ccaactcgcc gaggacgcgc tggtcatcgt
     7261 cagccttccc agcgtcccgg gtcccgtcac cgtggaaccc accctcgtgc actgcgacgc
     7321 cgtgttgatg acggtgctcg acgggtggac cccgatcgat gacgccatct tgacgatcga
     7381 ctcgctggtc gccgcggccg acgatcgcgt gggcctgctg ctcgtcgatc agtagcccgg
     7441 cgagcggtct gcccggtcag gtctcgatgg gccgataggc gaagcgcttc ttgaaccgga
     7501 gccacggctc ctcccagagc acccaggtaa tccgggcgat gaggagcgac gccccgaggg
     7561 tcaccccgaa actgacggca cttccgacga gttgaccgat gggtccgagc cggtcgccga
     7621 gtttcgacat cgtcgactgc atggtgtggg cgatcgggaa gtggaacaga tacatggcgt
     7681 agctgtactt gccgagcacc aggttgaacg gccaattcgt cgcccgttca acccgcggcc
     7741 cgagttggcc ctgtgccgcc cgaacgacgt acaggagaaa gaccaacgcg agcagcgtga
     7801 gccccggccc gtacgcgaag ttcggaaaca cgtccgacac ggtccagatg ggcatgagcc
     7861 cgatcagcag gatcggggcc atgacgatcg ggcgggccac ccaaccgaga atcgtgccga
     7921 tgaactcccg ggccttgggg tcgcgcaggt acagcgcacc acacgctccg atgagcaggg
     7981 tgtcgagccg gttgaacgta tattcgcggg cgccgtaggt gttgatcacc ccgccccaca
     8041 gcagggcggt tcgcaaggtc atcgagccga ggatcccgat gcagaaccat cgcagccgtg
     8101 atggtgcggg aacgaaatac atcacgacgg gccagaacag gtagaactgc tcctcgacgg
     8161 cgagggacca caggtggccg aggcccggaa gctggtgtgg gccgcggccg agcacgaagt
     8221 tctgtaggta gacccacacg gtccacagtc cgtccggtcc gttctggagc tcgaagaact
     8281 cctcgggcac cgtgacgaac gacggtaggt gattcacgat cactggtgct acgaagagca
     8341 tgagcaccaa aaacaggtag tacagcggga agatccgtac cgcgcgacgc gcgatgaaat
     8401 tgcggaaata ccgcgggtga tcccgggttt cgatgaggat cccggtgatc aggaagcccg
     8461 acaacacgaa gaacaggtcg accccgcgct cgccgaacac ggcccaggac agccggctgt
     8521 cgaaactgtg aaacacgagg gtcaacaaga tggcgacgcc tcggacccca tcgagtgagg
     8581 tgattcgggg gagcgcagcg ccgtcgtcgt gaacttgcgc cgtattcgaa acgaccaccg
     8641 atgcatcctc tcattcgtcg gtcgtgctcc cgacttcgtt cggcgaagtg tagaggaccc
     8701 gtgctcgatc ccggtgttgg cgaggatcgc tgcccctggt gggctgcggc gtactacggt
     8761 gcggggtacc ggcgtgtcgg cgccggctcg gccagtgcca acgcgacgac ccgtcggcga
     8821 gcaccggggc cccgcatatt ggtgtgagtt ccaaaggacg agccatggat ccgaccgatt
     8881 cgctcgacga tattttgcgt cctcagcgat tttctgaacg cgtccgcgcc cgttgggcca
     8941 cgccgtcggc cgaccgcgcc ccctcagcgt cggttcggct tgtcgccgtc gcggtcgccg
     9001 tggtggtggg ggttgcgacc gtcgtggcga tcgtggcgcg tccgccgcaa tcggtggggg
     9061 tcgccacatt gcctcaagcc tccgtgcccg atgtcggtgg cgaacgggcg ccgtcggacg
     9121 cgtcctcggg cgcggttggt gagggcgctg gtggcgcggg tgctggtggc gcagctggag
     9181 cggaggctgg catcgctggg caaggcgctg cgggggcggg cgagtgggtg gtgcatgtcg
     9241 ccggtgcggt gctcgagccg ggcctcgtca cgttgtcgcc cggttcgcgg gtcggtgacg
     9301 ccatcgctgc cgcgggcgga ccggccgccg tcgccgacct cgacgccatc aatctggcgg
     9361 aagtcctggc ggacggggtg cgggttcgag tgccagcggt cggtgagact ccaccggcga
     9421 acgcctcggc cctcgacggg agcggcgctg cccgaaatgg cgccggctcg gcctccgggg
     9481 gcgacagtgg cctcatcaac atcaacaccg cctcggtcga ggagctccag caactgccgg
     9541 gggtcggacc gagcacggcg gaggcgatcg tggcccatcg cgacaagcac ggcccgttct
     9601 cctcccccga tgcgctcgac gacgtccggg gaatcggccc ggcgaagctc tcggcgctgc
     9661 tcgacctcgt cgcgacgtga gcgagcgtca gctcttcgtc gcggccgcgg cggtggttct
     9721 cggcgcacgg tacgccgttg ccgtgccgtt cgcggtggtg atgatggccg tcgtgggggc
     9781 ctgcgtcgcc tcggcttcgc ggccgcgaag cgacgccggg gtgcgattcg tcgccgccgt
     9841 ggcgattgcg gcgttcgcca tcagcgcaag ctttctggcc gcccgagcgg aggccggcgt
     9901 gtcgatcatc gacacctccc atgaggtgtc gggggtcgcg acgttggtct ccgaccccca
     9961 gcagcggttc ggttccagcg tcgtggagat gcgcctcgac gctcgccggt atcgcgcgat
    10021 catcccgggc gaactggcac ccacgatcgc cgatgcccgg atgggacaac gcgtccgcgt
    10081 ccgaggcacc gcggctgcga tcgacgggcc gaccgggtgg ctcaagagcc ggcacttcgc
    10141 cggtcaactc cgccttcgca cgttggagcg ggtcgattcc gggtcgtggc tgtggcgttc
    10201 ggccaatggg ctccaggcgt tggttgccgc cgggcagcgt ccgttggcag ccgacgagcg
    10261 agccttgttt ctcggcgtgg tcttcggtga cgaccggggg cagacggcgg tgcagcagta
    10321 tcggtttcgg accgccggca cggctcacct gatggcggtg tcggggcaga acgtggtgct
    10381 gttggtggcg ctggcgatgc cgctgctcga acgcctggcg cggcacgctc ggttcgtgtc
    10441 ggtgctggcg ctgatcggct ggttcgcggt gctcacccgt gcggaaccgt cggtgctgcg
    10501 ggcaacggtc atggtgggtc tcggagcgtt ggtgagctgg caggggcgct acgcgagcgg
    10561 gcttcgggtg ttgtgcgtcg ccgtgatcgc gttggtgctg gccgacccgc tgctggtctg
    10621 gtcgctcggg tttcggctct cggtgggggc cagtctcgcg ttgattacgt tgtcgcggcc
    10681 gctcatcgag gtactggtgg gtcctcgttg gttcgcttcg gcgctcgggg tttcgttggc
    10741 ggcgcaggcc ggcaccgctc cgatgatcgt gtcgaccttc gggccggtcg gcctgctgag
    10801 cccgctggtg aatctggtgg cggtcccgtt gggtggtgcg ctgatggtct ggggatcggt
    10861 cagcgggccg atcgccggtc tgttgggtcg tcccgtcgat acgttcgccg ccatcgtgtc
    10921 gcgggggttg atctgggggc tcgaggcgag tgcggccatc ggcgcctggt cgaggctgcc
    10981 gaagctcggg gtgatcggga cactcgccat gtccgcgttg tgcgtgtggc cctggttacg
    11041 aacccttcat gtgcggctgt cgacggcgcg tctggttgcc tggtggctcg tcgcggtggt
    11101 gatcgtcgac cttgtcgtcg gtgaggtccg tgccccgcaa ccgcaggttc gctccgcggt
    11161 cggatccgcg accttgtgga gtgtggggcg cacgaacgtc atggtgttcg acgggccggt
    11221 tcgcgacggc gccgcgctcg atctcctcgc cggcatgcgc cgcccggtcc tcgaggccgt
    11281 catcgtcgtc ggcggcggca aggtctctgc gggggcggtg tgggcgcttc ggcaggtgac
    11341 gaccgtgggc gtggtgttcg cgcaggacc
//
LOCUS       DAOOMA010000017        10787 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_6921_length_10787_cov_4.076314, whole genome shotgun sequence.
ACCESSION   DAOOMA010000017 DAOOMA010000000
VERSION     DAOOMA010000017.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 10787)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10787)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10787
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6921_length_10787_cov_4.076314"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..541
                     /locus_tag="PLP95_01050"
     CDS             <1..541
                     /locus_tag="PLP95_01050"
                     /inference="COORDINATES: protein motif:HMM:NF014639.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="disulfide bond formation protein B"
                     /protein_id="HPB44418.1"
                     /translation="TLMVVAAYLAGLSGLALVVTSFFESGRRLVASLIGGQEMAFAWA
                     AAILMSASSLALSEVFHYNPCRWCWFQRVLAYPNVVVLGWGWFRKDRNSWAPALTLAG
                     LGLLTSGWHMLIDYGVISEGTSCDPYNPCTTRWKGFLSDYNTIQLCAFMCFLFIIGLG
                     LHALRNRPTTIAPEGTTSG"
     gene            534..1301
                     /locus_tag="PLP95_01055"
     CDS             534..1301
                     /locus_tag="PLP95_01055"
                     /inference="COORDINATES: protein motif:HMM:NF012787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HPB44419.1"
                     /translation="MAKKPNIQYGSPNGGVQPSTGNSKPKGVIIAVGAAIAVALIAII
                     VFAVQDGGGEGVNNTAFQPVTITGESLPALSDSGADPAIGMTIPTIVGKDFDGNTVEL
                     KPGEPTVVVIMAHWCAHCQQEIPSIVKWTNAGDLSSDVKVVGISTAVNESNGNFPPAS
                     WLQREAWTFPTIADSKRNESLGALGVSGYPTMIAVRADGTVGLRMSGEQPLSAAQQLW
                     AAALGEEPSTTDTIAPGPSSDATEDLPDEVATTTTAP"
     gene            1307..2092
                     /locus_tag="PLP95_01060"
     CDS             1307..2092
                     /locus_tag="PLP95_01060"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin family protein"
                     /protein_id="HPB44420.1"
                     /translation="MDPATPEPTPWASPDPPSPPPQGSETPPKRRGGIYLAAAAALLA
                     VAIAAIAVNSSDDAAGQRSTPQPPATDLPRSTIVASDVAAQPATISGDPLPTDLGESS
                     DPALGRPLPRVEGRSFDEAPVAVGNADVDGAGDPSAAEPTVVVVMAHWCPHCQAEIPR
                     IVDAYSRGELPLGVRVVGVATANDPLRGNFPPAAWLRRESWPFATMVDDEDGSVSHAL
                     GVTGFPTLIAVDADGKVALRASGEQTREELIGLWTAALGAITD"
     gene            complement(2111..2770)
                     /locus_tag="PLP95_01065"
     CDS             complement(2111..2770)
                     /locus_tag="PLP95_01065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB44421.1"
                     /translation="MPATVLVAAVALLTTACIPDVPSTGRATPVRRVLVLGDSLSFGL
                     FGTSPTIHGDLQQRMNPSGIDVRVIGGPGSNPIAPWPGLAPWNDQLSYAVATFDPDVV
                     IIQSVLFPEFVTPEGEARYLGAITSLFDIAQSQGAHTYIVAHHPPTNEREFLAAIEAE
                     RLQAQAGAGRGISTIPATWWMQRCDKAFLFDGFHLAANGVGCLADAMNAAVNQLRNEV
                     G"
     gene            2854..3915
                     /locus_tag="PLP95_01070"
     CDS             2854..3915
                     /locus_tag="PLP95_01070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020378905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HPB44422.1"
                     /translation="MGQDQGRSSPRWRALNALQQALYYLDRSAAPGQKVRAFMRAAQV
                     ISALDEAELDRLVAAGTITSLPGIGSSTGAVVADAVAGRSGGYLDRLDQTTRVEAAEG
                     AELRRRLRGDCHSHSTWSDGSAPVDLMARSAQSLGHDYLVVTDHSPRLTIAHGLSAER
                     LGEQRREIDELNARFDSEASARGHAAFRVLRGSEVDILTDGTLDLDDSVLGELDVVVA
                     SVHSKLRMPHDEMTARLIKAVSNPHVDILGHCTGRQISGTPRAPSTFDAAAVFRACAE
                     HNTAVELNCRPERQDPPDELLSVALDAGCRFVIDTDAHAPGQLEWLASGCDKAARHAI
                     DPTRILNTYGVEELLASLG"
     gene            complement(3879..4850)
                     /locus_tag="PLP95_01075"
     CDS             complement(3879..4850)
                     /locus_tag="PLP95_01075"
                     /inference="COORDINATES: protein motif:HMM:NF015587.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HPB44423.1"
                     /translation="MGWSVEQVRERVDDLQRRTPLIGFPLAVVKKYGDDKGSNLSALI
                     TYYAFFSLFPLLMVAFTILGFVLEGNPELQERIADTISERLPLPGVSADTITGNGLAL
                     VIGLALALWSGLGATQVAQDALNTVWDVARSKQPGFLRKRLQGLLVLAVIGVGIVAAT
                     AASSVAAIFGSWAAPAGIVGAFVINAAMMAMLTRVMVERRIEFGDLKWAAVVGGIGWT
                     ALQSIGGWYTARLVSNADKTYGTFAVVIGLLSWIFLQVQVFLYASEVASVSSRRLWPR
                     SIDVARPTEADEAVANAIIARSTLIRPGHDPVGSQVQPSEASSSSTP"
     gene            complement(5078..5722)
                     /locus_tag="PLP95_01080"
     CDS             complement(5078..5722)
                     /locus_tag="PLP95_01080"
                     /inference="COORDINATES: protein motif:HMM:TIGR00697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:1990397 - queuosine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="queuosine precursor transporter"
                     /protein_id="HPB44424.1"
                     /translation="MDQRVVWLIVLVSVYAAASLIANVMSIRAVSIFGWAVDAGTLTY
                     PLTFTIRDLIHKVGGKRAATTAIYTTVALNVAMVAAFWVAAALPPDMAVGSQEAFGEV
                     LNSAWRIVVASLVAQLVAELADTEIYQRYTDRFGQRAQIGRVLTSNAVSVPLDSVIFA
                     TIAFAGTFPAAVIVEIVAANIVIKVASSFVTAPLIYAVPGPRPSTGSGSPTGEV"
     gene            5808..6491
                     /locus_tag="PLP95_01085"
     CDS             5808..6491
                     /locus_tag="PLP95_01085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB44425.1"
                     /translation="MNQRPGPSLIELDLPGSPTTWSDLGFVVVDDRITLDNLVLVVGS
                     PEPRLALDPVPDGVGDWLGLPVRPISGQRHGGQTSADEAGATSATSATTATPNRITGV
                     DHIVIMTPEERSTVESIAMLGWEIRRERLSTIAGVDAVQRFVWAGAVLLEIVTPVARN
                     QESDEEPVAAQVWGVSFASSDLDETVSFLGPRSGPPRAAVQPGRRIATLQRGVVDHRL
                     HVAIMTARA"
     gene            6682..7572
                     /locus_tag="PLP95_01090"
     CDS             6682..7572
                     /locus_tag="PLP95_01090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB44426.1"
                     /translation="MRTKRWKTLFIGGLAATSLIAASCTSETPSTGRTFKATKTTVNS
                     VNDNWNAICAFTSNGGTGDEVKVLNIGFRVKMGVPGSASSHVAVGANPWPGAADCGPG
                     TGGTYTYTGAQQAAVTFNNISAPDVLHLVQGAPLEITGVWAWKLEDDGMLAADLTGAA
                     NTVAAALTSTLNATVAAGSVPSDANAIVQTVLDAIMSANFFNLFGAALGNISAFADDA
                     MGSGMYIGIGSSGTLANIIDSTAGQIQFPAIQLPVMVVPPDIGGGAIFSTKDNKTFVN
                     DATNGGISGNHTTTYTWSAS"
     gene            7719..9518
                     /locus_tag="PLP95_01095"
     CDS             7719..9518
                     /locus_tag="PLP95_01095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HPB44427.1"
                     /translation="MSEYRAPIKDALFTLRHIGQIDDLAATERFSHVDTETVQSVLEE
                     HGRFMQEVFAPLNRLGDTEGVSLNDGVVSHPAGFKEAYDKFVAAGWQGLNAEVEYGGA
                     GFPEAVFAITGEFMIAANGAFSMAPGLTTGAIECLQHWGTEEQKETYLQRMVTGEWAG
                     TMNLTEPQAGSDVGLATTKAEPQGDGTFKISGTKIFISFGEHELTENIIHLVLARLPG
                     APAGTKGISLFLVPKFLVNEDGSLGERNDVTCVSLEHKLGIHASPTCVLSFGDNGGAI
                     GYLVGQENQGMRAMFTFMNSARIAVGIQGIGLGDAAYQKALQYSTERKQGKEIGGASA
                     EPANIIVHPDVRRMLLTMRSQVEAMRALAVYNGLAIDLSRSLSDEAERTEWSEIAELL
                     TPVTKAWCTDVGMDVTSTAIQIYGGMGFIEESGVAQHFRDMRIAPIYEGTNGIQAMDL
                     VGRKLPMRMGGVVNDHFARIDATIADLEADASLAALATPLRDALGVLRETTNWIFENG
                     LGDPKQALAGATPYLTMFGIVTGGWLSARLALGASADLAAGADDEAYLRARMTSARFF
                     IEQLVPRAAGLKASVTAGASVLYEVDAAMLATR"
     gene            9597..10193
                     /locus_tag="PLP95_01100"
     CDS             9597..10193
                     /locus_tag="PLP95_01100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012227305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB44428.1"
                     /translation="MLDHVFTDAIGALRDALERALLERQALEERFQSDVLLGDLTWET
                     SYSLPGEGAPPRIQVDLSLAWPTWSQTAYRSWYLEEELDDPPHIDIEIVVRIQRLSAP
                     PDPRIVLDALPTESPPIGGERLHRSGPTLESAYSDDLEIVEHAIEVSYDGSYELDENV
                     LADGGNLDTDFAAMGGWISATLVRLGDLAMPHLPTADD"
     gene            complement(10221..>10787)
                     /locus_tag="PLP95_01105"
     CDS             complement(10221..>10787)
                     /locus_tag="PLP95_01105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015439658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HPB44429.1"
                     /translation="ALFDTVLRLQDSFTALERARMPVLAAIQGGCIGGGVDMVCAADM
                     RYATKDAFFCVQEINIGMTADVGTLQRLPKLVPDGIAREWAYTGDRISAERAKAAGLV
                     NEVFDDHEQLLEGVREIAGRIAAKSPLAIWGTKEQVLYARDHSVVDSLRHMAGWQSGM
                     FQPGDMMEEFAAKSEGRIASHAPLHRRP"
BASE COUNT         1946 a         3710 c         3386 g         1745 t
ORIGIN      
        1 aacgctgatg gtcgtcgcgg cctaccttgc cgggctctcg gggctcgcgc tcgtcgtcac
       61 gtcgttcttc gagagcggtc gacgcctggt ggcgagcctc atcggtggcc aggagatggc
      121 ctttgcctgg gcggcggcca tcctcatgtc cgcgtcaagc ctcgcgctca gcgaggtctt
      181 ccattacaac ccctgtcgct ggtgctggtt tcagcgggtg ctcgcctacc cgaacgtggt
      241 cgtgctcggg tggggatggt tccgcaagga tcgcaactca tgggctccgg cgctcacgct
      301 cgccggactc ggcctgctca cctcgggatg gcacatgttg atcgactacg gcgtcatctc
      361 cgaaggcacc tcgtgtgacc cttacaaccc gtgcaccaca cggtggaagg ggttcctcag
      421 cgactacaac accattcagt tgtgcgcgtt catgtgcttc ttgttcatca tcgggctcgg
      481 tctgcacgcg ttgcggaacc gacccacgac catcgccccg gagggcacca ccagtggcta
      541 agaaaccgaa catccaatac ggctccccca acggcggagt ccagccgagc accggaaact
      601 ccaagcccaa gggcgtcatc atcgccgtcg gggcggccat cgccgtggcg ctcatcgcga
      661 tcatcgtgtt cgccgtccag gacggcggcg gcgagggcgt gaacaacacc gccttccagc
      721 cggtgaccat caccggcgaa tcactgcccg cgctgtccga ctcgggggcc gaccctgcca
      781 tcggaatgac gattccgacc atcgtcggca aggacttcga tggcaacacc gtcgagctga
      841 agcctggcga acccaccgtc gtcgtgatca tggcgcactg gtgtgcccac tgccaacagg
      901 agatcccgag catcgtgaag tggaccaacg ccggcgacct cagctccgac gtcaaggttg
      961 tcggcatctc cacggcggtc aacgagtcca acggcaactt cccaccggcg tcctggcttc
     1021 aacgcgaagc ctggaccttt ccgaccatcg ccgattcgaa acgaaacgaa tccctcggag
     1081 ccctcggggt cagcgggtat ccgacgatga tcgcggtgcg agccgacggc accgttggct
     1141 tgcgcatgtc gggagagcaa cctttgtccg ccgcacagca gctttgggcg gcggctctgg
     1201 gtgaggagcc ctcgacgacc gacaccatcg ccccgggacc gtcgagcgac gcgaccgaag
     1261 acctccccga tgaggtggcg accaccacga cggccccatg atgccgatgg atccggcgac
     1321 gcccgaaccg accccatggg cttcgccgga tccgccgagt cctcctccgc aaggctctga
     1381 gacgccacca aagcggcgcg ggggaatcta cctcgctgcg gctgcggccc tgctcgcagt
     1441 ggccattgcc gccatcgcgg tcaactcctc agacgatgcg gccggccaac ggtcgacccc
     1501 tcagccgccg gcaaccgacc ttcctcgtag caccatcgtc gcctccgatg tcgccgctca
     1561 accggcgacg atttcgggag acccactgcc caccgacctc ggcgaatcga gcgatcccgc
     1621 gctcggccga ccccttcccc gggtcgaggg ccggtcgttt gacgaagcac cggtcgcggt
     1681 cggcaatgcc gacgtcgacg gtgccggtga tccgagcgcc gcggaaccga cggtcgtggt
     1741 ggtcatggcg cactggtgcc cgcattgcca ggcagaaatc ccccggattg tcgacgccta
     1801 cagccgcggg gaactccccc tcggggtgcg cgtcgtcggc gtggcgacgg cgaacgatcc
     1861 cctccgtggg aactttccac cggcggcttg gttgcgccgc gagtcgtggc cgttcgcaac
     1921 gatggtcgac gacgaggacg ggtcggtctc ccacgcactc ggggtgacgg ggttccccac
     1981 gctcatcgcg gtcgatgccg acgggaaggt cgcgttgcgc gcttcgggcg aacagacccg
     2041 cgaggagctc atcggactct ggaccgcagc actcggggcg atcaccgact gacggctctg
     2101 tcggccagtg tcagccgacc tcgttgcgca gctggttcac cgctgcgttc atcgcatcgg
     2161 ccaggcagcc gacgccgttg gctgcgaggt gaaacccgtc gaagaggaac gccttgtcgc
     2221 aacgctgcat ccaccaggtc gccgggatgg tggagatgcc gcgaccggca ccagcctgcg
     2281 cttgaaggcg ttcggcctcg atcgccgcga ggaactcgcg ctcattcgtc ggggggtgat
     2341 gcgccacgat gtacgtgtgg gccccttggg actgggcgat atcgaacagc gacgtgatcg
     2401 cgccgagata gcgggcctcg ccctcggggg tgacgaactc gggaaacagc accgattgga
     2461 tgatcacgac gtccggatcg aacgtggcca ccgcatagga cagctggtca ttccacggcg
     2521 ccagcccagg ccacggtgcg atcggattgc tgcccggtcc cccgatcacc cgaacatcga
     2581 tcccgctggg gttcatccgc tgttggaggt caccgtggat ggtcggcgag gtgccaaaca
     2641 gcccgaagga cagcgagtcg ccgaggacga gaacgcggcg caccggcgtc gcccgtccgg
     2701 tggagggcac gtcggggatg caggcggtcg tgagcagcgc cacggctgcg acgagcacgg
     2761 tcgccggcac tgcgaatcgg ttcggggacc tgtgagttcg gggagccatc atggcgggca
     2821 acgctaggcg aggccgcgac ctaccatggc gcggtgggtc aggaccaagg ccgaagctcc
     2881 cctcgttggc gagcgttgaa cgcgttgcaa caggcgctgt actacctcga tcgttcagcg
     2941 gccccgggcc agaaagttcg agcgttcatg cgcgccgcac aggtcatctc cgcgctcgac
     3001 gaggccgaac tcgaccggct ggtcgccgcc ggcacgatca catcgctgcc gggcatcggt
     3061 tcgtccaccg gcgccgtcgt cgccgacgcc gtggccggcc ggagcggcgg ctacctcgat
     3121 cgactcgatc aaacgacccg cgtcgaggcc gccgagggag ccgagctgcg gcgacggctg
     3181 cgtggagatt gtcattcgca cagcacctgg tccgacggct cggccccggt cgacctcatg
     3241 gcacgcagcg cacagtcgct cggccacgat tacctcgtcg tcaccgacca ctctcctcgc
     3301 ctcaccatcg cccacgggct gtcggccgaa cgcctcgggg agcaacgccg cgaaatcgac
     3361 gaactcaacg cccgcttcga ctccgaggcc agcgcccgcg gccacgctgc gttccgcgtc
     3421 ctacgcggca gcgaggtcga catcctcacc gacggcaccc tcgacctcga cgattcggtc
     3481 ctgggcgagc tcgacgtcgt cgtcgcatcg gtgcattcga agctccgcat gccccacgat
     3541 gagatgaccg cacgactgat caaagcggtg tccaaccctc acgtcgacat tctcggccat
     3601 tgcaccggcc gccagatcag cggaactccc cgcgctccgt cgaccttcga tgccgccgcg
     3661 gtctttcgcg cctgcgccga acacaacacc gcggtcgaac tcaactgtcg gccggaacgc
     3721 caggatccgc ccgacgagtt gttgtccgtt gccctcgatg ccggttgccg gttcgtgatc
     3781 gacaccgacg cccacgcacc cgggcagctc gaatggctgg cctccggctg cgacaaggcc
     3841 gcgcgccacg cgatcgatcc gacgcgaatc ctcaacacct atggcgtcga ggaactgctg
     3901 gcctcgctcg gctgaacctg cgaaccgacc gggtcgtgtc ccgggcggat gagggtcgag
     3961 cgggcgatga tcgcgttcgc caccgcctcg tccgcctcgg tcggccgggc gacgtcgatc
     4021 gagcgtggcc agagtctccg tgaggacacc gatgcgacct ccgacgcgta caagaacacc
     4081 tgcacctgaa ggaagatcca cgacaacaat ccgatgacga ccgcgaaggt gccataggtc
     4141 ttgtcggcgt tggacacgag ccgtgccgtg taccagccgc cgatcgactg aagtgcggtc
     4201 cacccgatgc cgccgaccac ggcagcccat ttcaagtcgc cgaattcgat tcggcgctcg
     4261 accatcacgc gggtgagcat cgccatcatc gccgcattga tcacgaacgc cccgacgatg
     4321 ccggccggag ccgcccatga gccgaagatg gccgcgaccg acgaagcggc ggtggcggcg
     4381 acgatgccga cgccgatcac ggcgagtacg agcagtccct gcagccgctt gcgcaaaaag
     4441 ccgggctgtt tggatctcgc cacgtcccac accgtgttga gcgcgtcctg ggcaacctgg
     4501 gtggctccga gccccgacca caacgccaac gccaaaccga tgacgagtgc cagcccgttg
     4561 ccggtgatcg tgtcggccga caccccggga agcgggaggc gttcggagat cgtgtcggcg
     4621 atccgttcct ggagttcggg gttgccctcc aggacgaagc cgaggatggt gaacgctacc
     4681 atcaacagcg gaaacagcga gaagaacgcg taataggtga tcaacgccga cagattcgat
     4741 cccttgtcgt ctccgtactt cttcaccacc gcaaggggga acccgatcag cggagtgcgc
     4801 cgctgaaggt catcaacgcg ctcccgcacc tgttcaaccg accagcccat cgctgcctcc
     4861 cgattcgtcg tcgcccgcga acacgctgcg ccgatgctaa gccctaacgt cgcccccgca
     4921 gacaacccgc agacccctct ctgcgcggct ttggcgccgc ggcgcattga cgctagggtt
     4981 gtcccctatg tcgaagcgga ccagcaagaa gaaggccaac acccgtcgca agaaggcgaa
     5041 tcacggccgc aagcccaacc agggccgcaa ctcctgatca aacctcaccg gtcgggctcc
     5101 cgctgcccgt cgagggtcgc ggacctggca cggcatagat cagcggcgcg gtaacgaacg
     5161 acgatgcgac tttgatgacg atgttcgcgg ccacgatctc cacgatgacc gccgcgggaa
     5221 acgttccggc gaacgcgatc gtcgcgaaga tgaccgagtc gagcggaacc gacaccgcgt
     5281 tggaggtcaa cacccgaccg atctgagcgc gctggccgaa ccggtcggtg tagcgctgat
     5341 agatctcggt gtccgcgagt tcggcaacca actgagcgac cagcgacgca acgacgattc
     5401 gccacgccga gttgagcacc tcgccgaagg cctcctgcga accgaccgcc atgtccggcg
     5461 gcagcgctgc ggccacccag aaggctgcga ccatggcgac attcagcgcc acggtggtgt
     5521 agatcgccgt cgtcgcggcg cgctttcccc cgaccttgtg gatcaggtcg cggatggtga
     5581 acgtcagcgg ataggtcagg gtgccggcgt cgacggccca accgaagatc gacaccgcgc
     5641 gtatcgacat gacgttcgcg atcaggctcg ccgcggcgta gacgctcacc aacacgatga
     5701 gccacacgac acgctggtcc acggggtcga cgtctctcga tcgtggcgcg ctcggtaccg
     5761 tcatcagatc cacgcggcaa cgctacccgc tgggccacac tggcccagtg aaccagcgcc
     5821 ccggaccatc actgatcgaa ctcgacctgc cgggttcgcc cacgacttgg agcgacctcg
     5881 gattcgtcgt ggtcgacgat cggatcacgc tcgacaacct ggttcttgtc gttggttccc
     5941 cggaaccgcg gttggccctc gatccggttc ccgacggggt cggcgactgg ttgggcctgc
     6001 cggtgcgccc gatctctggc caacggcacg gcggccagac cagcgctgac gaggccggcg
     6061 cgacgagcgc cacatcggcg acaacggcga cgccgaatcg aatcacgggc gtcgaccaca
     6121 tcgtgatcat gacccccgag gagcgctcca cggtcgagtc gatcgccatg ctgggatggg
     6181 agatccgccg cgagcgtctc tccacgatcg cgggcgtcga tgccgtgcag cgtttcgtgt
     6241 gggctggtgc ggtgctgttg gagatcgtca cgccggtcgc gcggaaccag gaatccgatg
     6301 aggaaccagt cgcggctcag gtctggggcg tgtcgttcgc gtcgagcgat ctcgatgaaa
     6361 cggtgtcgtt cctgggaccg aggagtggac cgccgcgggc agcggttcag cccgggcgca
     6421 gaatcgccac cttgcagcgc ggcgtcgtcg accaccgcct ccatgtcgcc atcatgacgg
     6481 cgcgtgcctg acggcgcatg cctgacggca cggcatcttc ggtctcctta aggctccgct
     6541 gaccgattca atgggagact gatgaggttt tgagggattt gcttgcattt cgggcgggaa
     6601 ctcgggctaa actgtcatac ggcgtggtaa ctctcgcgtc aggggcgccg gtgcggccgg
     6661 ccgaatctgg aggaaatgac agtgagaaca aagcgatgga agacactgtt catcggcggc
     6721 ctcgcagcga cgtcgctgat tgcggcttcg tgcacgagcg agacaccctc gacaggtcgt
     6781 acgttcaagg cgaccaagac cacggttaac agcgtcaatg acaactggaa cgcgatttgt
     6841 gcgttcacct caaacggtgg caccggcgac gaggtcaagg tgctcaatat cgggttccgc
     6901 gtcaagatgg gcgtccccgg ctcggcatcc tcccacgtcg cggtcggtgc caacccgtgg
     6961 cccggggcgg ccgattgcgg acccggcacc ggaggcacct acacctacac cggcgcccag
     7021 caggcggcgg tgaccttcaa caacatttcc gctcccgacg tcttgcacct ggtgcagggc
     7081 gctccacttg agatcaccgg cgtgtgggcc tggaagcttg aagacgacgg catgctcgcg
     7141 gccgacctca ccggtgccgc gaacaccgtc gctgcggccc tcacctcgac gctgaacgcg
     7201 accgtggctg ccgggtcggt cccctccgac gcgaacgcga tcgtccagac ggtgctcgac
     7261 gcgatcatga gcgcgaactt cttcaacctg ttcggcgccg ccctgggcaa catctcggcc
     7321 ttcgccgatg atgcgatggg ttcgggcatg tacatcggca tcggatcgag cggcaccctg
     7381 gccaacatca tcgactcgac cgccggccag attcagttcc ccgcaatcca gcttccggtg
     7441 atggtcgttc cgccggacat cggtggcggc gccatcttct cgacgaagga caacaagacc
     7501 ttcgtcaacg acgcgaccaa cggtggcatc agcggcaacc acaccaccac ctacacgtgg
     7561 tcagcatcct gatcatgtaa atgagtgacg agagcccggg gcatccgccc cgggctctcg
     7621 tcgttttggc cgggagtccc ggacaccact cccaccacgt cacggctccc acccggcact
     7681 gtttggcctc gatgttaccg accggtaaca tcgaggccat gtcagagtac cgggcaccca
     7741 tcaaggacgc gctgttcacc ctccgccaca tcggccagat cgatgacctc gcggcgaccg
     7801 agcgcttctc ccacgtcgat accgaaaccg tgcagtccgt gctggaggag cacggccgat
     7861 tcatgcagga ggtcttcgct ccgttgaatc gtctcggcga caccgagggc gtctccctca
     7921 acgacggtgt cgtctcccac cccgccggct tcaaggaggc ctacgacaag ttcgtcgcag
     7981 cggggtggca ggggctcaac gccgaggtgg aatacggcgg tgcgggattc cccgaggcgg
     8041 ttttcgccat caccggagag ttcatgatcg ccgccaacgg cgcattttcg atggcgccgg
     8101 ggctcaccac gggtgccatc gaatgcctcc agcattgggg cacagaggag cagaaagaga
     8161 cctacctgca aaggatggtc accggcgaat gggccggcac catgaacctc accgagcccc
     8221 aggccggctc cgacgtgggt ctcgccacca ccaaggccga accccaggga gacggaacct
     8281 tcaagatctc cggcaccaag atcttcatct ccttcggcga acacgaactc accgagaaca
     8341 tcatccatct cgtgctggca cgtcttcccg gagcaccggc ggggacgaag ggcatctcgt
     8401 tgttcctggt ccccaagttc ctcgtgaacg aggacggctc cctcggcgaa cgcaacgatg
     8461 tcacctgtgt gtcgctcgag cacaagctcg gcattcacgc gtcacccacc tgcgtgctga
     8521 gcttcggcga taacggcggt gccatcggtt acctcgtcgg ccaggagaac caggggatgc
     8581 gcgccatgtt caccttcatg aacagcgctc gtatcgccgt cggaatccag ggaatcgggc
     8641 tcggcgacgc cgcgtaccaa aaggcgctgc aatactcgac cgaacgcaag cagggtaagg
     8701 agatcggcgg cgcgtcggcc gaaccagcga acatcatcgt gcaccccgac gttcggcgga
     8761 tgctgttgac gatgcgtagc caggtcgaag ccatgcgtgc cctcgcggtg tacaacggcc
     8821 tcgccatcga cctttcccgt tcgttgtccg acgaggccga gcgcaccgag tggtccgaaa
     8881 tcgccgaatt gctgaccccg gtcaccaagg cgtggtgcac cgacgtcggc atggacgtca
     8941 cctccaccgc catccagatc tacggcggca tgggcttcat cgaggaatcc ggagtcgcac
     9001 agcatttccg cgacatgaga atcgccccga tctacgaggg gaccaacgga attcaggcga
     9061 tggatctcgt cgggcgcaag ctgccgatgc gcatgggcgg cgtcgtcaac gaccatttcg
     9121 cccggatcga cgcaacgatc gccgatctcg aggcagatgc ctcgctcgcc gcgctcgcca
     9181 cgccgttgcg cgacgccctc ggcgtgctgc gcgagaccac caactggatt ttcgaaaatg
     9241 gtctcggcga tcccaaacaa gcgctcgccg gcgcaacccc gtacctcacc atgttcggga
     9301 tcgtcacggg cggatggttg tcggctcgac tcgcactcgg agcctcggcc gatcttgcgg
     9361 ccggcgcgga cgacgaggcc tacctccgtg cccgcatgac gagcgcgaga ttcttcatcg
     9421 aacaactcgt ccctcgcgca gccggactca aggcatcggt gaccgccggc gcctcggtcc
     9481 tctacgaggt ggacgcagcg atgctcgcga cgcgctgaag cagcagcatt tgcggcgatg
     9541 gcggacgggg cgacccgtcc gccatcgccg ttttcggcgc tatggtgagc gacgcaatgc
     9601 tggatcacgt cttcaccgat gccattggag cgctgcgcga cgcattggag cgcgcgctgc
     9661 tcgaacgcca ggcgctcgag gagcgctttc agtcagacgt gctgctcggc gacctcacct
     9721 gggaaacctc ctactcgtta cccggcgagg gggcaccacc acgcatccag gtcgacctgt
     9781 cgctggcgtg gccgacctgg tcacagaccg cctatcggtc gtggtacctc gaagaggaac
     9841 tcgacgaccc tccgcacatc gacatcgaaa tcgtggtgcg gatccaacgc ctttcggccc
     9901 cacccgaccc caggatcgtc ctggacgcgt tgccgaccga aagcccgccc atcggcggcg
     9961 aacgacttca tcgaagcgga ccgacgctcg agtccgccta cagcgacgat ctggagatcg
    10021 tcgaacacgc catcgaagtg agctacgacg gctcctacga actcgacgag aacgtgctgg
    10081 cggacggcgg aaacctcgac accgacttcg cggcgatggg cgggtggatc agcgcgacgc
    10141 tagtccggct cggcgacctg gcgatgcccc atctgccgac cgccgacgac tgaacctccc
    10201 cgcagacttg ttgacccggc ctacgggcgg cggtgaaggg gtgcgtgcga cgcaatacgg
    10261 ccttcggact tcgcagcgaa ctcctccatc atgtcgcccg gctggaacat gccgctttgc
    10321 caaccggcca tgtgccggag gctgtcgacc acactgtggt cgcgggcgta caggacctgt
    10381 tccttggttc cccagatcgc cagcggcgac ttggcggcga tccggccggc gatctctcga
    10441 accccctcga gtaactgctc gtgatcgtcg aagacctcgt tgacgagccc cgccgccttc
    10501 gctcgctcgg cggagatccg atcgccggtg taggcccact cgcgggcgat gccatcgggc
    10561 acgagcttcg gaaggcgttg cagcgtgccg acatctgcgg tcatgccgat gttgatctcc
    10621 tgcacgcaga agaaggcatc cttggtcgca tagcgcatgt cggcggcgca gaccatgtcg
    10681 accccaccgc cgatgcagcc gccctggatc gccgccaaca ccggcatcct cgcccgctcg
    10741 agggcggtga agctgtcttg caatcgaaga accgtgtcga acagcgc
//
LOCUS       DAOOMA010000018        10506 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_7180_length_10506_cov_4.436418, whole genome shotgun sequence.
ACCESSION   DAOOMA010000018 DAOOMA010000000
VERSION     DAOOMA010000018.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 10506)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10506)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10506
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7180_length_10506_cov_4.436418"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1001
                     /locus_tag="PLP95_01110"
     CDS             <1..1001
                     /locus_tag="PLP95_01110"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HPB44430.1"
                     /translation="ATTNTSQLSMAPLLATAIENNSMNAALVASLGGLGRGSTATGAA
                     SPLPTGHDVITTASKYLGVDYRWGGTDPETGLDCSGLIQRVYGELGIELPRVSRQQAT
                     SGTAVESLDQARPGDILAFGQPVNHVGLYVGGGKMLHAPRSGEVVKIGDITREIVAIR
                     RVIPTASDAAAALSLLGAANPVASPRALGVGASSSASLGASMAGAAPYASLFETAGAK
                     YGFNPTLLAAIAKTESNFDPTARSPVGAQGLMQFMPATAAGLGIDPSDPAQAIDGAAR
                     YLRQQLDRFGSIELALAAYNAGPGNVAKYGGIPPFTETRNYVAKVTGLLGGASPLR"
     gene            1081..2520
                     /locus_tag="PLP95_01115"
     CDS             1081..2520
                     /locus_tag="PLP95_01115"
                     /inference="COORDINATES: protein motif:HMM:NF014207.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-length control protein FliK"
                     /protein_id="HPB44431.1"
                     /translation="MSTDLLAFAPAAAAPPVAQRRSRDATTSNGGAAFGREFRRVTSK
                     TAGTTSDKAGQAASTTTSTSDDSPDTDAASADESLNSPAEVAVLVALTAQGLQVAPTA
                     VDDSAAVAAAATGQVAPAGTAHAHSATPTTDAPSESALDSATVASTAHDPTSTASSAT
                     TPPAPAEAALTIAAAVASSETHDVEPDAVNTGRTPSAGTAGSTTRTAAPNPTQPSAMT
                     AELVAGSRGPETTPASAANTSASTEGHPRSGEDPADTADPPPTGAHTAPMTTGSPSDG
                     QSALEPGFAALTRASTPPVATDGSTRAPDPLAEVATRVIAEARLAELETALAQHGPNG
                     TKILGASRLLRGGPGAPSTLTINLDPAALGKIRVELSLHEGQVALSLHADKAQAVQAL
                     NAQMPQLRDTLEADGVRLAHLGVDLSQQGSPHHGGGQATSSSDHTSTARSTDSATDSL
                     TDTDQHTDTAPPTTRRQRPGGSASVEVDL"
     gene            2530..2937
                     /locus_tag="PLP95_01120"
     CDS             2530..2937
                     /locus_tag="PLP95_01120"
                     /inference="COORDINATES: protein motif:HMM:NF015896.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook capping FlgD N-terminal
                     domain-containing protein"
                     /protein_id="HPB44432.1"
                     /translation="MTTPISSTAAASYSIDSLNGEGKAVSNPKGQLDNQAFLNLLVAQ
                     LRFQDPMKPTDSAALMQQTSTLSQVEMIKDMTAAQHLSTASALIGRTVTYIDAATGVE
                     ASGVVESAGKVAGKTTLQVSGKEAELTSVTRIS"
     gene            3002..4276
                     /locus_tag="PLP95_01125"
     CDS             3002..4276
                     /locus_tag="PLP95_01125"
                     /inference="COORDINATES: protein motif:HMM:TIGR03506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook protein FlgE"
                     /protein_id="HPB44433.1"
                     /translation="MIRSMYSAISGLRNHQTMMDVVGNNIANVNTTGFKSSSVVFQDV
                     LSQQYRGAGLATTTTGGTNPSAIGLGSKVAAISTNFGQGSLQATGRSTDFGIQGDGFF
                     ILSNPTGTSYTRSGSFAVDALGRLTTSDGSFVQGWQAQLDGTIPITQPIDKITIPVGD
                     LVPPIQTQVVTVGGNLPAGAADGTVFTTAASIFDGQGNPVSLRLEFTKVAGVPDTWQA
                     EARYVDGAGGLQPAPPAAGIGLGSNLVFDASGKLATPASGQLTIPAGSGLPGFGTQAV
                     TIDLASESPINQYGTIESVAILVQDGSAAGSLQTFSVSHEGLIVGTYSNGRTRIIGQF
                     ALATFANPGGLTASGGTNYKESVNSGIPQVGTPGGGAGRGLVSSGTLEMSNVDLSMEF
                     TNLIVAQRGFQANSRVITTSDEMLQELVNLKR"
     gene            4424..4705
                     /locus_tag="PLP95_01130"
     CDS             4424..4705
                     /locus_tag="PLP95_01130"
                     /inference="COORDINATES: protein motif:HMM:NF018043.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar FlbD family protein"
                     /protein_id="HPB44434.1"
                     /translation="MILLTRLNGSELGLNPDLIERVESTPDTVIALIDGTKYVVSEPT
                     TEVIARVIEFRARVIAAAQRHNDRIIADPAPLHLVTDRHGVAAPGTQEY"
     gene            4705..5487
                     /locus_tag="PLP95_01135"
     CDS             4705..5487
                     /locus_tag="PLP95_01135"
                     /inference="COORDINATES: protein motif:HMM:NF013760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="motility protein A"
                     /protein_id="HPB44435.1"
                     /translation="MDPATLLGLALVLVGVFVGAILDGVSPMTFFATPAAFLIVLLSA
                     LGATFFSNTMADAKALPKVLIKGIKGQKLRDNGELIDQIVSFAEMARREGLLALEDQA
                     KNIEDPFFRRGLQLAIDGADPDMVSDVMEADITAMSARHKQGSKMMTSIGIYSPTFGI
                     IGAVIGLIHTMHLLDSPSELGQGIAGAFTATFWGVFAANGIFLPLGNKLAVMSAVEIA
                     QKRLIAEGVLSIQSGANPRLLDDMLRSSLPPSERGDADKKSA"
     gene            5519..6427
                     /locus_tag="PLP95_01140"
     CDS             5519..6427
                     /locus_tag="PLP95_01140"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein MotB"
                     /protein_id="HPB44436.1"
                     /translation="MSKKKKHHEEHEEHVNHEAWVIPYADMLTLLMALFLVMWASSQV
                     DVNKAREVAAGFADSLHIVGSGDGVGGKGVFDGGVQPDVKPDLLTGQALQALEESKLN
                     AAAHAAALEQLEAVRQQIEQGAAEAGVANDVTFRNEERGLIVSVAAEGVLFDAGSAAL
                     RIDGLVVLDRLATVIAAIPNDVIVEGHTDNTPISTALFPSNWELSTARATAVTRHLVD
                     QRGVPAQRITASGRGEHHPVADNATAEGRARNRRVDFVIVTEGLVVATSNTAAFDTDV
                     APASPFDTAPTSSNPTAHAPKEGSHE"
     gene            6420..6923
                     /locus_tag="PLP95_01145"
     CDS             6420..6923
                     /locus_tag="PLP95_01145"
                     /inference="COORDINATES: protein motif:HMM:NF015693.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body-associated FliL family
                     protein"
                     /protein_id="HPB44437.1"
                     /translation="MSDTTEAAETGADADEGAKKAKKDKGNKGGKSNTVPAIILALGL
                     LGGGYFMGSGGSAEAAEHPTTTLAPVLGEIATMEPINVNLADGHFLRVGVALQLIEGV
                     EKKEFEAGATSKANDLLISQLGGRSMAELASAEGRELIKTELKEHLKETYEGEVVDVF
                     FTEFVMQ"
     gene            7009..7995
                     /locus_tag="PLP95_01150"
     CDS             7009..7995
                     /locus_tag="PLP95_01150"
                     /inference="COORDINATES: protein motif:HMM:NF013236.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliM/FliN family flagellar motor switch protein"
                     /protein_id="HPB44438.1"
                     /translation="MTTHSADPRRTNRDPRAVFGDGMHQFSPADFEVVARRFAELTFP
                     LTAGWSNLLGSEVLINVSEVTPGPDPTSETLDPSSSATLLDNVAGAGSYRVIATFGPD
                     LDAIAVVVPTALGLTVVDRLLGGTGRTIDNRRLTDIDRDLLSTVLEPTFGAIGELRPS
                     GQGHLPIGRMTETIESELNDRLAAGSTVTITVVIGDERLPLHIVLGPAATRELAGVRS
                     QSTHTTPLSKSESELKMQSALASVSVEVVVAFEPIKLPSHRVLALDVGDIISLRMTPD
                     SALPLFVDERRLAEVRPARSGTQVACQVVSTFRDGTPDSSATPDSHTFGGSL"
     gene            7992..8855
                     /gene="fliN"
                     /locus_tag="PLP95_01155"
     CDS             7992..8855
                     /gene="fliN"
                     /locus_tag="PLP95_01155"
                     /inference="COORDINATES: protein motif:HMM:TIGR02480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliN"
                     /protein_id="HPB44439.1"
                     /translation="MSTGIDSTATFVDDLSELLQRGTVGLIGDARLAGPAAPQDSAAE
                     LVPDLASGCVGIGLVYGDDIVLAITLTNGFASEFAGETTPTALQHALSDTIAELAGAR
                     HEQLAQVVGLENEHDLVEFLSDRPGLVGAGVFDGDNVVATLGAAGPALATAPTQVPTQ
                     APATTPDPIIPDIAFAEPGAPAIEPTVSDVVPESSTRPEVDDHRLARGLALLADVNLE
                     VTAELGRTRLRVSELLSLEPGSVIGLEREAGSPVDLLVNGTLFARAEVIVVDNQYAVR
                     ITELVGGGVPA"
     gene            8852..9322
                     /locus_tag="PLP95_01160"
     CDS             8852..9322
                     /locus_tag="PLP95_01160"
                     /inference="COORDINATES: protein motif:HMM:NF016250.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliO"
                     /protein_id="HPB44440.1"
                     /translation="MSADTFLLLVRLGFSLALVFGLMWVAGRVVKGRSPLAARRNDDH
                     VEIIERKALSRNTSVAVVRVGDNVFALGVSESNVTVLGEVTVEANLDGTAQPTVVELD
                     AVSMGPAHIPVSTSAIGSPADGGHAATRPATTRPIEGRGWLDSLRDKTVRHIPS"
     gene            9329..10249
                     /gene="fliP"
                     /locus_tag="PLP95_01165"
     CDS             9329..10249
                     /gene="fliP"
                     /locus_tag="PLP95_01165"
                     /inference="COORDINATES: protein motif:HMM:NF009438.0"
                     /note="The bacterial flagellar biogenesis protein FliP
                     forms a type III secretion system (T3SS)-type pore
                     required for flagellar assembly; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system pore protein
                     FliP"
                     /protein_id="HPB44441.1"
                     /translation="MSESLHATTNRLMLRRIGVIIVVCVAMLGWFTASASGASAQATP
                     APPRAQTTPAASIPEIGDAGPGDDKITIDLDFGSMFGDTDAKSGPDGTGADSNAGPSR
                     SIVILIMLTLLSLVPSLLVCMTSFTRIVVVLSIARNAIGLQSIPPNQVVTGLALFLSL
                     FVMAPTIKEINDNAFQPYLKGDMEQSVAFKEAEGPIKNFMLSNTRNDELNLMIKASDS
                     ERPDDLHDLSLMTVIPAFILSELKSAFIIGVMVLLPFLVIDLVVSAGLMSLGMMMLPP
                     TFVSLPFKLLLFVMVDGWALIAQSLIANFK"
     gene            10273..>10506
                     /locus_tag="PLP95_01170"
     CDS             10273..>10506
                     /locus_tag="PLP95_01170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015797630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliQ"
                     /protein_id="HPB44442.1"
                     /translation="MTDSAVTHIGIQTMILTTKLAAPVLLTALAVGVLIGLVQSATQL
                     QESTIAFVPKFAAIGIVLIITGNWMLSEAITFTH"
BASE COUNT         1925 a         3762 c         3130 g         1689 t
ORIGIN      
        1 ccgccaccac gaacacatcc cagctgtcga tggctccgtt gctcgcaacc gcgatcgaga
       61 acaactcgat gaacgccgcg ctcgtcgcgt cgctcggggg tttgggaaga ggatccaccg
      121 ctaccggagc agcttcgcca ctccccaccg gacacgacgt catcaccacc gcttcgaagt
      181 acctcggcgt cgactatcgc tggggtggga ccgaccccga aaccggcctg gactgttcgg
      241 gactcatcca acgtgtctat ggcgaactcg gcatcgagct gccgcgggtc agccgccaac
      301 aggcaacgtc cggcacggcg gtggaatcgc tggaccaggc ccgccccggc gacatcttgg
      361 cgttcggaca accggtcaat cacgtcggac tctacgtggg tggcggaaag atgcttcacg
      421 cgccacgtag cggcgaggtt gtgaagatcg gcgatatcac ccgagagatc gtcgccatcc
      481 gtcgagtgat tcccaccgca agcgacgcgg cagcggcgct gagcctcctc ggtgccgcca
      541 accccgtcgc gtccccccgg gccctggggg tcggtgccag ttcctccgcc tcgctgggcg
      601 cgtcgatggc cggcgccgca ccatacgcgt cactgttcga gaccgccggt gcgaagtacg
      661 gcttcaatcc gacgctgctc gcggcgatcg ccaagaccga gtccaacttc gacccgaccg
      721 ctcgaagccc cgtcggtgct cagggcctca tgcagttcat gccggcaacc gccgccgggt
      781 tgggcatcga cccgagcgac ccggcgcagg ccatcgatgg tgcagccagg tacctccgcc
      841 aacaactcga tcgcttcgga tccatcgaac tggcgttggc cgcctacaac gcgggccctg
      901 gaaacgtcgc gaaatacgga ggcatcccgc ccttcaccga gacccgcaac tacgtcgcca
      961 aggtgaccgg cctcctcggg ggcgcctcac cgcttcgttg accccccgac aacggtcatc
     1021 cacaccagct ttccactccc cgtagccacc ccccttgcga gattccgaga accgagaacc
     1081 atgagcaccg atcttcttgc attcgcccct gccgccgcgg ctccaccggt cgcgcaacgc
     1141 cggtctcgcg acgccacgac atcgaacggg ggcgccgcct tcggacgcga attccggcgg
     1201 gtgacgtcca agaccgctgg aacaacgagc gacaaggccg gtcaggccgc gtcaaccacg
     1261 acgtcaacct ccgacgattc gccggacacc gatgccgcat cggctgacga atcgttgaac
     1321 tcacccgctg aggtcgcagt gttggtggcg ctgaccgccc agggcctgca ggtggctccg
     1381 acggcggtgg acgacagcgc tgctgttgct gcggcggcga ccggccaggt ggcccccgcc
     1441 ggcacggctc acgcgcacag cgccaccccg acgaccgacg caccctcgga atcggcactc
     1501 gattcggcga cggtcgctag caccgcccac gaccccacct caaccgcttc gtcggccacg
     1561 acaccacccg ccccagccga ggcggccctc actatcgctg cggcggtcgc ttcctccgag
     1621 acccatgacg ttgaacccga cgccgtgaac accggtcgca caccgagcgc cggcaccgca
     1681 ggctcgacca cgcgaaccgc cgcgccgaat ccaacccagc cgtccgccat gaccgccgaa
     1741 ctcgtcgctg ggtcacgagg tcccgaaacg acgccggctt cagccgccaa cacgtccgcc
     1801 tcaaccgagg ggcacccccg gtccggcgag gatccagccg atacggcgga cccgccgccc
     1861 accggagccc acactgcacc gatgaccacc gggtcaccgt cggacggcca atcggcgctc
     1921 gaacccggct tcgcggcgct caccagggct tcgacgccac cggtggcgac cgacggatcc
     1981 acccgcgccc ccgatccgtt ggccgaggtt gccactcggg tcatcgccga ggcccggctc
     2041 gccgaactgg aaaccgcgct cgctcaacac ggtccgaacg gaaccaagat tctcggcgct
     2101 tcccgacttc tccgaggcgg gcccggcgct cccagcacgc tcacgatcaa cctcgacccg
     2161 gcggcgctcg gcaagatccg cgtcgagctc agcctccatg agggtcaggt tgccttgagt
     2221 ctccatgccg acaaggccca ggcggtccaa gcgctcaacg cccagatgcc ccagctacgc
     2281 gacaccctcg aggccgacgg ggtacggctc gcccacctcg gcgtcgacct ctcacagcag
     2341 ggttccccgc accacggtgg cggccaggcc acatcttcga gcgaccacac ctcaactgcg
     2401 cgaagcaccg attcggcgac cgattcgctg accgacaccg atcagcacac cgacaccgcg
     2461 ccgccgacca cgcggcgcca gcgccccggc gggtccgcgt ccgtcgaggt cgacctctaa
     2521 ggagcagcca tgaccacacc catctcttca accgccgctg cgtcgtactc gatcgattcg
     2581 ctcaacggcg aaggcaaggc cgtgagcaat ccgaaggggc aactcgacaa ccaggccttc
     2641 ttgaacctgc tcgtcgctca actccggttc caggatccga tgaagccgac cgactccgcc
     2701 gcactcatgc agcaaacctc gacgctcagt caggtcgaga tgatcaagga catgaccgcc
     2761 gcacagcacc tctctacggc gagcgcgctg atcggacgaa cggtcaccta catcgatgca
     2821 gcgaccgggg tcgaagcctc cggcgtcgtc gaaagcgccg gcaaggtcgc cggtaagaca
     2881 accctccagg tgagcggcaa ggaagcggaa ctcacctccg tcaccaggat cagctgaccg
     2941 gctgacccgc acaccacggc ccgcaccaca cggtccaccc tctcacctcc aggagtaatt
     3001 catgatccgc tcgatgtatt ccgccatctc cggccttcgc aaccatcaaa cgatgatgga
     3061 cgtcgtcggc aacaacatcg ccaacgtcaa caccaccggc ttcaagtcgt cgtcggtggt
     3121 cttccaggac gtgctcagcc agcagtaccg cggcgcaggc ctggcgacga ccacgaccgg
     3181 cggcaccaac ccgtcggcca tcgggctcgg ctcaaaggtc gccgccatct ccacgaactt
     3241 cgggcagggc tcgctacagg ccaccgggcg ttccaccgat ttcggcatcc aaggtgacgg
     3301 cttcttcatc ctgagtaacc cgaccggcac gagctacacc cgctccggct ccttcgcagt
     3361 cgacgcgctc ggacggctca cgaccagtga cggctcgttc gtccagggct ggcaggcaca
     3421 gctcgacggc acaattccga tcacccagcc gatcgacaag atcacgatcc cggtcggcga
     3481 cctcgtgccc ccgatccaga cccaggtggt caccgtcggc ggcaaccttc ccgctggagc
     3541 ggcagacggc acggtgttca ccaccgcggc gtcgatcttc gacggccagg gcaaccccgt
     3601 cagcctgcgg ctcgagttca ccaaggtcgc cggggttccc gacacctggc aggccgaggc
     3661 acgctatgtg gacggtgcag gcggcctgca gccggcacct ccggccgccg gtatcggact
     3721 cggttccaac ctggtgttcg acgcgagcgg caagctcgcc acgccagcga gtggacagct
     3781 cacgattccg gcaggcagcg gcctgcccgg gttcggaacc caggccgtca ccatcgatct
     3841 ggcctcggaa agcccgatca accagtacgg caccatcgaa tcggtcgcca tcctcgtcca
     3901 ggacggctcg gcggccggtt cgctgcagac cttctccgtt tcccatgaag gtctcatcgt
     3961 cggaacctac tccaacggcc gcacacgaat catcggccag ttcgccctcg ccaccttcgc
     4021 caacccggga ggtctcaccg cttcgggtgg caccaactac aaggagtcgg tgaactcggg
     4081 aattccccag gtcggcaccc ccggaggcgg ggcgggacga ggacttgtgt cctcgggcac
     4141 cctcgagatg tccaacgtcg acttgagcat ggaattcacc aacctgatcg tggctcaacg
     4201 gggattccag gccaacagtc gcgtgatcac gacctcagac gagatgctcc aggaactcgt
     4261 caacctcaag cgctgacact gaccgggcgc gccccgccgg ggcgcgcccg gtcacctgag
     4321 aaccgtcccg gagatatcgg gtcaacgtct cagcttcgcg acgaaacgtc cgatgtaaca
     4381 accaccaccg tcgccctgtc gcggccctgc caacggatct cccatgattc tcctcactcg
     4441 cctcaacgga tcggaactcg gcctcaaccc cgacctgatc gaacgcgtcg agtcgactcc
     4501 cgacaccgtc atcgcgctga tcgacggaac caagtacgtc gtttcggagc cgactaccga
     4561 ggtgatcgcc cgcgtgatcg agttccgcgc cagggtcatc gccgccgctc aacgacacaa
     4621 tgaccgcatc atcgccgatc cggcacccct gcatctcgtg accgaccgtc atggggtcgc
     4681 cgcacccggc acacaggagt attgatggat ccagcaaccc ttctcggact cgcgttggtg
     4741 ctcgtgggcg tcttcgtcgg agccatcctc gacggcgtca gcccgatgac cttcttcgcc
     4801 acgccggcgg cgtttctcat cgtgttgctc tccgccctcg gtgcgacgtt cttctccaac
     4861 acgatggccg atgcgaaggc actgcccaag gtgctgatca aaggcatcaa gggccagaag
     4921 ctccgcgaca acggcgagtt gatcgaccag atcgtcagct tcgccgagat ggcccgccgc
     4981 gagggactgt tggcccttga ggatcaggcc aagaacatcg aggacccgtt ctttcgtcgc
     5041 gggctacaac tggcgatcga cggggccgac cccgacatgg tgagtgacgt catggaggcc
     5101 gacatcaccg ccatgtcggc ccgccacaag caggggtcga agatgatgac ctcgatcggc
     5161 atctattcac cgaccttcgg catcatcggt gccgtcatcg gcctcatcca cacgatgcac
     5221 ctactcgatt caccctccga actgggccaa ggcatcgccg gcgcgttcac cgcgacgttc
     5281 tggggcgtgt tcgcagcaaa cggcatcttc ctgccgctcg gaaacaagct ggcggtgatg
     5341 tcggcggtgg agatcgcaca gaagcgcctc atcgccgaag gggtgctgtc gatccagtcc
     5401 ggggcgaacc cgcgcctgct cgacgacatg ttgcgcagct cgctgccgcc gtcggaacgg
     5461 ggcgacgccg acaagaagag cgcctaggcc ggcgtcaccg tcgcctcaag gatgagaagt
     5521 gagcaagaag aaaaagcacc acgaggaaca cgaggaacac gtcaatcacg aggcatgggt
     5581 cattccctat gccgacatgt tgacgttgtt gatggcgctg tttctcgtca tgtgggcatc
     5641 gagccaggtc gacgtcaaca aggcccgcga ggtcgcggcc ggctttgccg actcactgca
     5701 catcgtcggc agcggtgacg gcgtcggcgg caagggggtt ttcgacggcg gggttcaacc
     5761 cgatgtgaaa cccgacctcc tgaccggcca agccctccag gcgcttgagg aatcaaagct
     5821 caacgccgca gcgcacgcag cggccttgga gcaactcgaa gcggtccgcc aacagatcga
     5881 acagggcgcc gccgaggccg gcgttgcgaa cgacgtcacc ttccgtaacg aggaacgcgg
     5941 actgatcgtg tccgttgcgg ctgagggggt gctgttcgat gccgggtcgg cggcgctgcg
     6001 catcgacgga ctcgtcgtgc tcgaccggct cgcgaccgtg atcgcagcga tccccaacga
     6061 tgtgatcgtc gagggacaca ccgacaacac gccgatttcg acggcactgt ttccttcgaa
     6121 ctgggagctg tcgaccgcga gggctaccgc ggtgacgcga cacctggtcg atcaacgcgg
     6181 cgtgcccgca cagcgaatca cagcatccgg acgcggtgag catcaccccg tcgccgacaa
     6241 cgccaccgcc gaaggccgcg cccgcaaccg tcgggtcgac tttgtcatcg tgaccgaggg
     6301 cctcgtcgtc gcaacgtcca acaccgccgc cttcgacacc gacgtcgccc cggcgagccc
     6361 gttcgacacg gctccaacca gctcaaatcc cacagctcac gcacccaagg aggggtccca
     6421 tgagtgacac gaccgaagca gccgagaccg gcgcagacgc cgacgaaggc gcgaagaagg
     6481 cgaagaagga caagggaaac aagggcggaa agtccaacac cgttccggcg atcatcttgg
     6541 ccttgggcct ccttggtggc ggctacttca tgggttctgg cggaagcgcc gaagccgcgg
     6601 aacaccccac gacaacgctg gcgccggtgc tcggcgaaat cgcgacgatg gaaccgatca
     6661 acgtcaacct ggccgacgga cactttctcc gggtcggtgt ggcgttgcag ctcatcgagg
     6721 gcgtggagaa gaaagaattc gaagcgggtg ccacgtcgaa ggccaacgac ctgttgattt
     6781 cccaactcgg cgggcggtcg atggcggaac tcgccagcgc tgagggtcgg gaactcatca
     6841 agaccgaact caaagaacac ctcaaggaga cctacgaggg tgaggtcgtc gacgtgttct
     6901 tcaccgagtt cgtcatgcag taagggcact gcgcgggcga ctgggtcatt cagcccagaa
     6961 atccgagatc agcgctcatc gttcggcccg gggtgccgat aggtacctgt gaccacccat
     7021 tccgcggacc ctcggcgaac gaaccgtgac ccacgcgccg tgttcggcga cggcatgcac
     7081 cagttctccc ccgccgactt cgaggtcgtc gcgcgtcgct tcgccgagtt gaccttcccg
     7141 ctgaccgccg ggtggtcgaa tctgcttggt tcggaggtcc tcatcaatgt ctccgaggtg
     7201 acccccggac cggatcccac ctcggagacc ctcgatccat ccagctccgc cacgctgctc
     7261 gacaacgtcg ctggcgccgg ctcctaccgg gtgatcgcaa ccttcggtcc cgacctcgac
     7321 gccatcgcgg tcgtggttcc caccgccctc ggcctgacgg tggtcgaccg gctcctcggc
     7381 ggaaccggac gcacgatcga caaccgtcgc ctcaccgaca tcgaccgcga cctgttgtcg
     7441 acggtgctgg aaccgacctt cggcgcgatc ggagagctgc gcccatccgg gcagggccac
     7501 cttcccatcg ggcgcatgac agaaaccatc gagtcggaac tcaacgaccg gctcgctgcc
     7561 ggctccaccg tgaccatcac ggtggtgatc ggcgatgaac gactgccgct gcacatcgtg
     7621 ctcggcccgg cggcgacgcg cgaactcgcc ggagtgcgtt cgcagtcgac ccacacgacc
     7681 ccgctgtcga aatcggagag cgaactcaag atgcagtccg cactcgcgtc ggtgagcgtc
     7741 gaggtcgtcg tcgcgttcga gccgatcaag ttgccctctc atcgcgtcct cgcgctcgat
     7801 gtgggcgaca tcatctcgct gcgaatgacg ccggactcgg ccctgccctt gttcgtcgac
     7861 gaacggcggc tcgcagaggt gcgtcccgcc cgctccggca cccaagtggc gtgtcaggtc
     7921 gtatccacct tccgcgacgg caccccagac agctcagcga cacccgattc ccacactttc
     7981 ggaggctcat tgtgagtacc ggtatcgatt cgactgcgac gttcgtggac gacctgagcg
     8041 agctgctcca gcgcggcacg gtcggcctga tcggagacgc tcgacttgct gggcccgctg
     8101 ccccgcagga ctccgccgca gaactggtgc ccgatctcgc gtcgggatgt gtcgggatcg
     8161 gattggtcta cggcgatgac attgtcctgg ccatcaccct gacgaacggg ttcgcgagcg
     8221 aattcgccgg tgagacgacc ccgacggcgc tgcaacatgc cctctcggac acgattgcgg
     8281 agttggccgg agcccggcac gaacagctcg cccaagtcgt cggcctcgaa aacgaacacg
     8341 acctggtcga gttcctgagc gaccggcccg gcctggtcgg tgccggggtc ttcgacggcg
     8401 acaacgtcgt cgccacgctc ggtgccgccg gtcccgcgct ggcgacggcc ccgacccagg
     8461 tcccgacaca ggccccggcc accacccccg acccgatcat ccccgacatc gccttcgccg
     8521 aaccgggtgc acccgctatc gagccaaccg tctccgacgt ggtgcccgaa tccagcaccc
     8581 ggcccgaggt cgacgaccac cgcctcgccc gcggcctggc gctcctcgcc gatgtcaacc
     8641 ttgaagtgac cgcggaactc ggcagaaccc gactgcgggt cagcgagttg ttgtcgctcg
     8701 aacccggctc ggtcatcggt cttgaacgag aggccggcag ccccgtcgac ctgctggtca
     8761 acggcaccct cttcgcccgc gccgaggtca tcgtggtgga caaccagtac gccgttcgaa
     8821 tcaccgagtt ggtgggcggt ggggttccgg cgtgagcgcc gacacctttc ttcttcttgt
     8881 gcgcctgggg ttctccctcg ccctcgtctt cgggctgatg tgggttgccg gacgcgtcgt
     8941 caagggccgt tcgccgctcg ccgctcgccg taacgacgac catgtcgaga tcatcgaacg
     9001 caaggcactg agccgcaaca cctccgtggc cgtcgttcgg gtcggcgaca acgttttcgc
     9061 cctcggggtc agcgaatcga acgtcacggt tctcggcgag gtcaccgtcg aggcgaacct
     9121 cgacggtacg gcgcagccga cggtcgttga actcgacgcc gtttcgatgg gaccggccca
     9181 tatcccggtg tcgacctctg cgatcggttc gccggcggat ggcgggcatg ctgcgaccag
     9241 gcctgcgacg acacggccca tcgaaggtcg cgggtggctc gacagccttc gcgacaagac
     9301 cgtccggcac atcccgagct gatccgtgat gtccgagtca cttcacgcca cgacgaatcg
     9361 actgatgctg cgccgaatcg gtgtgatcat cgtcgtctgc gtcgcgatgt tgggatggtt
     9421 caccgcgtcc gcgtccggcg cctcagcgca agcaacccct gcgccgccgc gagcgcagac
     9481 gacgcccgca gccagcattc ccgagatcgg cgacgccggc ccgggcgacg acaagatcac
     9541 catcgacttg gacttcgggt cgatgttcgg cgacaccgac gccaagagcg gacccgacgg
     9601 cacgggcgcc gacagcaacg ccggaccgtc gcgcagcatc gtcatcctga tcatgttgac
     9661 gttgctgtcg ctggtgccgt cactgctcgt gtgcatgacc agcttcaccc gcatcgtcgt
     9721 ggtgttgtcg atcgcccgaa acgcgatcgg cctgcagtcg atccctccca accaggtcgt
     9781 gaccgggctc gctttgttcc tctcgttgtt cgtcatggca ccgacgatca aggagatcaa
     9841 cgacaacgca ttccagccct acctcaaggg cgacatggag cagtcggtgg cgttcaagga
     9901 ggccgagggg ccgatcaaga acttcatgtt gtcgaacacc cgcaacgacg agttgaacct
     9961 gatgatcaag gcgtccgatt cggaacgccc cgacgatctc cacgacctgt cgctcatgac
    10021 ggtcatcccg gcgttcatcc tcagcgaact caagtccgcg ttcatcatcg gggtgatggt
    10081 gctgttgccg ttcctcgtca tcgacctcgt cgtcagcgcc ggcctgatga gcctcggcat
    10141 gatgatgctg ccgcccacgt tcgtttcgtt gccgttcaag ttgttgctgt tcgtgatggt
    10201 cgacggatgg gcgctcatcg cccagtcgct catcgccaac ttcaaatagc gcacccattc
    10261 ttcggagatt cgatgaccga ttccgccgtc acccacatcg gtatccagac gatgatcctc
    10321 accaccaagc tcgcggcacc ggtgctgctg accgcgttgg cggtcggcgt gttgatcgga
    10381 ctcgtccagt cagcgaccca gctgcaggag tcgaccatcg ccttcgtccc caagttcgcg
    10441 gccatcggca tcgtgttgat catcaccggc aactggatgc tgtccgaggc gatcaccttc
    10501 acccat
//
LOCUS       DAOOMA010000019        10464 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_7239_length_10464_cov_4.457200, whole genome shotgun sequence.
ACCESSION   DAOOMA010000019 DAOOMA010000000
VERSION     DAOOMA010000019.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 10464)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10464)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10464
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7239_length_10464_cov_4.457200"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..930
                     /locus_tag="PLP95_01175"
     CDS             <1..930
                     /locus_tag="PLP95_01175"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006635 - fatty acid beta-oxidation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HPB44443.1"
                     /translation="ANNRHCAAGLAAITTAAGSIRAGMDSVVVAGGAESASTAPRFTR
                     RVLGTDEWDAWAPPSHVQTPDAPIEDMSITVGWNAAVEAGVTREEMDYWAFRSHQRAI
                     EAIDAGSFTAEIAPIKVTLRDGSVTEFAVDEHPRRGTSLEKLASLKVIHPEIEGFSIT
                     AGNASGVNDGAAALVIVDDSVARSEGLESLGTVRAWASAGVTPARTGLAPTIAIPKVL
                     DRAGLSIGDVKLWEINEAFASMCVGTTKLLGIDDEIVNVEGSGCSLGHPVAMTGARMV
                     ISLVHELGRRGGGIGVAAMCAGGGMSTAVVVEV"
     gene            933..2147
                     /locus_tag="PLP95_01180"
     CDS             933..2147
                     /locus_tag="PLP95_01180"
                     /inference="COORDINATES: protein motif:HMM:NF012296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HPB44444.1"
                     /translation="MSFDETIDETELLEHFNHLAPEMGDRDYFHRALERLRGAANGVV
                     RSDQHEGFWVATRYEDVLAIAQDWEGFSSEHGITVPPRESPLPAIPEMVDPPLHREYK
                     RLINRFFTPVQVLEHEEATRAIVNQLIDEFITEGRCDFMEAFANPLPGLVFFEEFLHA
                     PSEELAEVNRLATAASTPNTPSAIDARREMIGWIFGFIERRRAAEPIDDVIDAVLNAE
                     IDGRAITDLEAVGIIQLLLFGGLDTTAGALGTMMLWFAKQPEIAQRLRAEPELIPTAI
                     EEMLRLDGPFAFIGRRATRDIEVGGAAICEGDMVVVSWAAANRDPNEFGCPAEFDLDR
                     EGNRHIAFGAGPHRCAGSNLARMNLRIAVEELLRRLSDVRLATGEVHFHPGYSRAPSA
                     IEIEFTPAERES"
     gene            2192..3355
                     /locus_tag="PLP95_01185"
     CDS             2192..3355
                     /locus_tag="PLP95_01185"
                     /inference="COORDINATES: protein motif:HMM:NF016777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HPB44445.1"
                     /translation="MYANDIRVIDADTHMTERHDLFTSRAPKGFEDRVPRVTMVDGTA
                     TWTLEGVPLGPARPGGVIDRNGDKHAFAETRAHGIDWVHQGAWDWQYRLGVMDETGID
                     VQIVYPNAVGIGGQNLAKATQDPVLRRLCVELYNDAMAEVQENTNGRMLPMPVMPAWS
                     IDECVAEAQRCAAMGFRGVNMTSDPQDAGSTDLADRAWDPFWEVCADLHLPIHFHIGA
                     SQTSLTFYGKYFWASLHENLKPAVGGSMLFLNNAGVVINSIYAGIFDRHPNLKMVSVE
                     SGIGWIPFILETMDYEILENAPEQADALGRRPSEYFKDHWWATFWFETNHGDLQGLID
                     KVGEDNVMFETDYPHPTCLYPDPLGTVSEIISTLRPETQRKVLGENAAKLYRV"
     gene            3384..4517
                     /locus_tag="PLP95_01190"
     CDS             3384..4517
                     /locus_tag="PLP95_01190"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HPB44446.1"
                     /translation="MLLEPIEDQAFLRDTTAKFLADAAPPQVLRTLRDDEAGFDEAYW
                     RQGVELGWTSLLVSEEAGGGSVSGDGLVDLSLIAHEFGAAAAPGPLAPNAVVAGALAR
                     SGGHDEVVAGVLSGGTLVSWAIDEDAHNVAAMPRLFRFRIEGTELVLDGTKRLVESAN
                     RSDYLLVTGVTPGGITQVLVATDAAGIEIRPMHTVDLTRRFSVVTFNNVRVPLDAVVG
                     SIDTAAEAVKLQRRQAIVIANAESVGAMQRAFDLTLEWTFDRYSFGRSLASYQAIKHR
                     MASMKMWLEASHAIADDAAAAVAADSPRAEKLVRAAAAYIGEYGSELMQECVQFHGGI
                     GVTYEHDLHLYLRRHTLNRALHGTPAQHRRRVAVELDLESAVS"
     gene            4514..5809
                     /locus_tag="PLP95_01195"
     CDS             4514..5809
                     /locus_tag="PLP95_01195"
                     /inference="COORDINATES: protein motif:HMM:NF014790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HPB44447.1"
                     /translation="MSTESMAPVSNTDTLDDLDEFRRRAKGFLRAEVGPARASLTAGL
                     RKLGTDEDDLREVAHDRALQRTFFDAGFAGITVPVEFGGQGLTRAHQLVLNEELEGYD
                     YPVRLQVPTMTPCMAVLLEFGTEQQKLTHIPAILRGEELWMQFLSEPSGGSDVAGALM
                     SAVRDGDEWVLNGSKVWTTGAWWSDWGLCLARTNWDVSKHRGLSVFIFPIDAAGIEIQ
                     RIEMLNGNMDFCQEYLDDVRVPDSDRVGGVDDGWTVGTRWMFHERMSSNSPFVTRPQE
                     LERSSLDGAALRRIARDGGISGTDAAQELVGEGEMLTLVGSALGRRVGQGMRTGTLSD
                     QSAAIARLYGGLSLVRRNTIALELAGSAGAAWSDDDGELALRGEDFLMRQASCIGGGT
                     TEMASNVIAERVLGMPRERALDRDVAFRDVPRGPASEEN"
     gene            5828..6583
                     /locus_tag="PLP95_01200"
     CDS             5828..6583
                     /locus_tag="PLP95_01200"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013530.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HPB44448.1"
                     /translation="MRVVILGASSGLGRSVAVGLGETGHDVALLARRVDRLEQTVADL
                     GERAHAIRCDVTDEAQCTEAVRAAADVLGGIDALVYAPGVAPLGRLTDLDADAWTRTL
                     TTNVVGAATATRAALDHLQASQGCAIYFSSVSASMTPAWPGLGAYIASKAALDKMVEA
                     WRVEHPEVGFTRLIVGDCGGGEGDSATGFTSEWDMDIAVELGRQWYAKGYVAGALIDV
                     NELVRAVESIISLGNTAVVPTMAVVPRVPRNDG"
     gene            6639..6920
                     /locus_tag="PLP95_01205"
     CDS             6639..6920
                     /locus_tag="PLP95_01205"
                     /inference="COORDINATES: protein motif:HMM:NF018563.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine/purine nucleoside phosphorylase"
                     /protein_id="HPB44449.1"
                     /translation="MALQHNTYFDGTVQSIGFVTGEGRSSVGVMDVGEYHFGTEAPER
                     MTVVAGELIVQLPGAPWATYVAGSSFEVPGNSAFDLKVKRPTAYLCDYL"
     gene            7007..8137
                     /locus_tag="PLP95_01210"
     CDS             7007..8137
                     /locus_tag="PLP95_01210"
                     /inference="COORDINATES: protein motif:HMM:NF024742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HPB44450.1"
                     /translation="MLVPVSLALPDQLATVDHAHRYRNFGLINALVGLAALVSLPLFG
                     AMCDRTNSRFGHRKAWVLGGVVAMVVGLILTGRQDTWQMIAFWWIVANLGNSAIATGL
                     TASVADQVPDDQRGTISGFMYGTQGLGIVVGMVAVEGFEAATRYVLLAIALVLCALPY
                     LVVNRDVEPSAGTEPLTLKRILSNMWISPRRYPDFGWAFGSRLLVNVGNALGTTYLWF
                     FLRDGLKVEDPDGTLITVTVIYLVATLIATFVAGPVSDRTGRRRIFVGVAAGLQAVAG
                     VLVAAAPSVGMLMVAAAFIGAGYGAFVAVDQALVTAVLPNADDRAKDLGILNVGAAVP
                     QGLGPLAAAGITTAFGFSPLFLAAGLSTAVGALMVSRVRSVR"
     gene            complement(8174..8770)
                     /locus_tag="PLP95_01215"
     CDS             complement(8174..8770)
                     /locus_tag="PLP95_01215"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: protein motif:HMM:NF014033.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_process: GO:0009235 - cobalamin metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP:cob(I)alamin adenosyltransferase"
                     /protein_id="HPB44451.1"
                     /translation="MTTRGGDHGETSLGDATRVSKTDARIEAIGAIDELNAVVALAAD
                     AVALAELPDADSDTGPDADTEPGSALNVTIAEVLEELLQQLFDLGADIALPEPGERGR
                     LITVDTVVWLENVTAAFNAPLSPLRSFILPGGPNGAGQLHFARTVCRRAERRVLALGG
                     DIGEAGRYLNRLSDVLFVLARTVASGDERLWAPTRNRE"
     regulatory      8868..9014
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            9086..9640
                     /locus_tag="PLP95_01220"
     CDS             9086..9640
                     /locus_tag="PLP95_01220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB44452.1"
                     /translation="MNTQSVIARPRRTRTLRLALAFVALVGIVTSACVSESTPSQPVP
                     VANIVGEPCMPGVGVTVVVDFQALDDRISIGCAVGPQTDGLAALTNAGFTINNKPGAP
                     ASTICQINNQPTAGYPVCWLQNYWSYWKSDGSKAWSFSEVGVAAGPLPIDSFEGWSYS
                     PFSEGDITGLKLRRTVNQIKALRG"
     regulatory      9735..9879
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            9996..>10464
                     /locus_tag="PLP95_01225"
     CDS             9996..>10464
                     /locus_tag="PLP95_01225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB44453.1"
                     /translation="MASEKLTHTTVPRSKALVLIPAIVAVSLLGACTPNGPGTPATTT
                     TSSTSTTTTTASPLPVCEVPEAPVLTLIRSNEVLTFTIADGSPIELAVIASDADPGDA
                     LYEEASTLSLAAYEGQVVRVLARSGGDCDPEVIFDATYDVRAQMPKRPGVAGTD"
BASE COUNT         1707 a         3365 c         3596 g         1796 t
ORIGIN      
        1 gctaacaacc gccattgcgc ggccggtctt gccgcaatca cgacggccgc ggggtcgatc
       61 cgcgccggta tggattcggt cgtcgtggcg ggcggagccg agtcggcctc caccgcgccg
      121 cgcttcaccc gacgggtgct cggcaccgac gaatgggatg cctgggcacc gccgagtcat
      181 gtgcagaccc ctgacgcccc gatcgaagac atgtcgatca ccgtgggctg gaacgccgcg
      241 gtcgaggccg gcgtgacccg cgaggagatg gactactggg cgtttcgttc gcaccagcgg
      301 gcgatcgagg cgatcgacgc cggcagcttc accgccgaga tcgctccgat caaggtcacc
      361 ttgcgcgacg gaagcgtcac cgagtttgcg gtcgacgagc atccccgccg gggtacgagc
      421 ctggaaaagc tggcctcgct caaggtgatc cacccggaga tcgaggggtt cagcatcacc
      481 gctggcaatg cctcgggggt caacgacggc gcagcagcac tcgtcatcgt cgacgattcg
      541 gtggcgcgca gcgaggggct cgagtcgctc ggcaccgtcc gggcgtgggc atcggccggg
      601 gtcaccccgg cacgaaccgg cctcgctccg acgatcgcca ttccgaaggt tctcgaccgg
      661 gcgggtttgt cgatcggcga cgtgaagctg tgggaaatca acgaggcgtt cgcgtcgatg
      721 tgtgtcggca cgacgaagct gttgggcatc gacgacgaga tcgtcaatgt cgagggcagc
      781 gggtgcagcc tcggtcaccc ggtcgccatg acgggcgccc gcatggtgat ttcgctggtc
      841 catgaactcg ggcgccgtgg tggcggcatc ggtgttgcgg ccatgtgcgc cggcggcggc
      901 atgtccaccg cggtggtggt ggaggtctga aggtgagctt cgacgagacg atcgacgaga
      961 ccgaactcct cgagcacttc aaccacctcg cccccgaaat gggcgaccgc gactacttcc
     1021 accgggccct cgaacggttg cgcggcgccg ccaacggtgt cgtgcgcagc gatcagcacg
     1081 agggcttctg ggtggccacc cgttacgagg atgtgttggc catcgcccag gactgggagg
     1141 gtttcagctc ggaacacgga atcacggttc cgccccgcga gtctccgctg ccggcgatcc
     1201 ccgagatggt cgacccgccg ttgcatcgtg agtacaagcg cctgatcaac cggttcttca
     1261 ccccggtgca ggtgctcgaa cacgaagaag cgacgcgggc gatcgtcaat cagctcatcg
     1321 atgagttcat caccgagggt cgctgcgact tcatggaggc gttcgccaat ccgttgcccg
     1381 gcctggtgtt cttcgaggag ttcctccacg cgccgtcgga ggaactcgca gaggtcaatc
     1441 gtctcgcgac cgcggcgtcg acgccgaaca ctccctcggc gatcgatgcc cggcgcgaga
     1501 tgattggatg gattttcgga ttcatcgaac ggcggcgcgc cgcagaaccc atcgacgacg
     1561 tcatcgacgc ggtgctcaat gcggagatcg acggacgtgc catcaccgat ctcgaagcgg
     1621 tggggatcat ccaactcctg ctcttcggcg gcctcgacac caccgccggc gcgctcggca
     1681 cgatgatgct ctggttcgca aagcagcccg agatcgcaca gcggctacgc gccgagcccg
     1741 aactcatccc gacggcgatc gaggagatgt tgcggctcga cggtcccttt gccttcatcg
     1801 gtcgccgagc cacccgcgac atcgaggtcg gcggggcagc catctgcgag ggcgacatgg
     1861 tggtcgtgtc gtgggcggcg gcgaaccgcg accccaacga gttcggctgc ccggccgagt
     1921 tcgacctcga ccgcgaaggt aaccgccaca tcgccttcgg tgctgggccg caccgctgcg
     1981 ccggatcgaa cctggcgcgc atgaacctgc gcatcgcggt cgaggagttg ctgcgtcggc
     2041 tgtccgacgt tcgcctggcg accggcgaag ttcacttcca ccccggttac agcagggcgc
     2101 cctcggccat cgagatcgag ttcaccccag cggaacgcga atcgtagatc tgtccgggca
     2161 cgccaccacc cacccaggag acgacccttt catgtatgcc aacgacatcc gagtcatcga
     2221 cgccgacacc cacatgacgg agcgtcacga cctgttcacg tctcgcgccc cgaagggatt
     2281 cgaagaccgt gtcccgcggg tgaccatggt ggatggcacc gcgacctgga ccctcgaagg
     2341 ggtgccgctc ggaccggcgc gcccgggtgg ggtcatcgac cgcaacggcg acaagcacgc
     2401 cttcgccgag acgcgggcac atgggatcga ctgggtccac cagggggcgt gggactggca
     2461 ataccgcctc ggggtgatgg acgagacggg catcgacgtg cagatcgtct acccgaacgc
     2521 ggtcggcatc gggggccaga accttgccaa ggcaacccag gatccagtgc tgcgacgcct
     2581 gtgcgtcgag ttgtacaacg acgcgatggc cgaggttcag gagaacacca acggccggat
     2641 gttgccgatg ccggtgatgc cggcgtggag tatcgacgag tgcgtcgccg aggcgcagcg
     2701 gtgcgctgcg atggggttcc gcggcgtgaa catgacgtct gatccccaag atgccgggtc
     2761 gaccgacctt gccgatcgcg cctgggatcc gttctgggag gtgtgcgccg acctgcacct
     2821 gccgatccac ttccatatcg gagcatcgca gacctcgctc acgttctacg gcaagtactt
     2881 ctgggcctcg ctgcacgaga acctgaagcc ggctgtcggc ggttcgatgc tgtttctcaa
     2941 caacgccggc gtggtcatca acagcatcta cgcgggcatc ttcgaccgtc acccgaacct
     3001 caagatggtg tcggtggaaa gcggcatcgg ctggattccg ttcatcctcg aaacgatgga
     3061 ctacgagatt ctcgagaacg ctcccgaaca ggcggatgcg ctcgggcgcc gaccgtcgga
     3121 gtacttcaag gaccattggt gggccacgtt ctggttcgag acgaatcacg gcgacctgca
     3181 ggggctcatc gacaaggtcg gcgaggacaa cgtgatgttc gagaccgact acccgcaccc
     3241 gacctgtctg tacccggatc cgctcggcac ggtgtcggag atcatctcca cgcttcgacc
     3301 ggagacccaa cgcaaggtgc tcggggaaaa cgccgccaag ctgtaccgcg tctgacacct
     3361 caagccagga ggggactcac ccaatgttgt tggaaccgat cgaagaccag gcgtttcttc
     3421 gcgacacgac cgcgaagttc ctcgccgacg cggcgccgcc gcaggtgctg aggacgttgc
     3481 gtgacgacga ggccggattc gacgaggcct attggcgcca gggggtcgag ctggggtgga
     3541 cctcgctgct ggtgagcgag gaggccggcg gcggttcggt gtcgggggac ggcctcgtcg
     3601 atctgagcct catcgctcat gagttcggtg cggcggccgc tccggggccg ctcgctccca
     3661 acgcggtggt ggccggggcg ctcgcccgca gtggcggcca cgacgaggtg gtcgccggag
     3721 tgctcagcgg ggggacgctg gtgtcgtggg cgatcgacga ggatgcccac aacgtcgcgg
     3781 cgatgccgcg gttgttccgg tttcggatcg agggcaccga gttggtgctc gacggaacca
     3841 agcggctcgt cgagtcggcg aaccgcagcg actacctgct cgtcaccggt gttacgccgg
     3901 gtgggatcac ccaggtgctc gtggccaccg acgccgcggg aatcgagatt cgtcccatgc
     3961 acacggtgga tctgacccgt cggttctcgg tcgtgacgtt caacaacgtg cgggttccac
     4021 tcgacgcggt cgtcgggtcg atcgacaccg cggccgaggc ggtgaaactg cagcgccgac
     4081 aggccatcgt gatcgcgaat gcggagtcgg tcggggcgat gcaacgggcc ttcgacctca
     4141 ccctggaatg gaccttcgac cggtactcct tcgggcggtc gctggcctcc tatcaggcga
     4201 tcaaacaccg catggcttcg atgaagatgt ggctcgaggc cagccatgcc atcgcggacg
     4261 atgcggcggc agcggtcgcg gccgacagtc cccgagcgga gaagttggtg cgggccgccg
     4321 ccgcgtacat cggcgaatac ggctcggaac tcatgcagga atgcgtacag ttccacgggg
     4381 gaatcggggt cacctacgaa cacgatctgc acctgtacct acgtcgccat acgttgaacc
     4441 gggcgctgca cggaacccct gcccagcacc gccgtcgggt cgcggtggaa ctcgatctcg
     4501 aaagcgccgt ctcatgagca ccgaatcgat ggctccggtc tccaacaccg acaccctcga
     4561 cgacctcgac gagttccgac gtcgggccaa gggattcctg cgcgccgagg ttggcccggc
     4621 gcgggcgagc ctcaccgccg ggctacgcaa gctcggaacc gacgaggacg acctgcgcga
     4681 ggtcgcgcac gaccgggcgc tgcagcgcac cttcttcgac gccgggttcg ccggaatcac
     4741 cgtgccggtg gagttcgggg gtcaggggtt gacccgggcg catcaactcg tgctcaacga
     4801 ggaactcgag ggctacgact atccggttcg gctgcaggtt cccaccatga cgccgtgcat
     4861 ggcggtgctg ttggagttcg gcaccgagca gcagaagctc acccacatcc cggcgatcct
     4921 gcgtggcgaa gagctgtgga tgcagttcct gtccgagccg agcggagggt ccgacgtggc
     4981 gggagcgctg atgagtgcgg tgcgcgacgg cgacgaatgg gtgctgaacg gttcgaaggt
     5041 gtggaccacc ggggcctggt ggtcggactg ggggctgtgt ctggcccgaa cgaactggga
     5101 cgtttccaaa caccgtgggt tgtcggtgtt catcttcccg atcgacgctg ccggcatcga
     5161 gatccaacgc atcgagatgc tcaacggcaa catggatttc tgccaggagt acctcgacga
     5221 tgtccgggtt cccgacagcg accgggtcgg cggtgtcgac gacgggtgga cggtcggaac
     5281 ccggtggatg ttccacgagc ggatgtcgtc gaactcgccg ttcgtgaccc gaccacagga
     5341 gctcgaacgg tcgtccctcg acggtgcggc cctgcgccga atcgcccgcg acggcggcat
     5401 ttccggcacc gatgctgcac aggaactcgt cggcgagggg gagatgttga ccctggtcgg
     5461 atcggcgctg ggccgccgtg tcggccaggg gatgcgcacc gggaccctga gcgaccagtc
     5521 ggcggcgatc gcccggctgt acggggggtt gagcctcgtg cgacgcaaca cgatcgcgct
     5581 tgaactcgcg ggatccgccg gtgcggcctg gagcgacgac gatggcgagt tggcgttgcg
     5641 aggcgaggat ttcctcatgc gccaggccag ctgcatcggt ggcggcacga cggagatggc
     5701 ctccaacgtc atcgccgagc gggtgctcgg gatgccgcgg gaacgggcgc tcgatcgcga
     5761 cgtggcgttc cgcgacgtcc cgcgcggacc cgcatcagaa gagaactgaa ccacggaggt
     5821 ttatcgaatg agagtcgtga tcttgggagc gtcgagcggg ttgggtcgct cggttgcggt
     5881 cggactcggc gagaccggcc acgacgtggc gctgttggcc cgtcgcgtcg atcgtctcga
     5941 gcagaccgtg gccgacctcg gcgagcgcgc gcatgccatt cgttgcgatg tcaccgacga
     6001 ggcgcagtgc accgaggccg tccgggcggc cgccgacgtg ctcggcggaa tcgatgcgct
     6061 ggtgtatgcg cccggagtcg ccccactcgg ccgtctcacc gatctcgacg ccgatgcgtg
     6121 gacgcgcacc ttgaccacca acgtcgtcgg tgccgccacc gccacccgcg ctgcgctcga
     6181 ccatctccag gcgtcgcagg gctgcgcgat ctacttctcc tcggtgtcgg cctcgatgac
     6241 cccggcgtgg cccggcctcg gcgcctacat cgcgagcaag gccgccctcg acaagatggt
     6301 cgaggcgtgg cgagtcgaac accccgaggt cggcttcaca cggctcatcg tgggcgactg
     6361 cggcggcggc gagggggatt cggcaaccgg gttcacctcc gaatgggaca tggacatcgc
     6421 cgtcgaattg ggccgacagt ggtatgccaa ggggtacgtc gctggagcgt tgatcgacgt
     6481 gaacgaactg gtgcgggccg tggagagcat catctcgctc ggcaacaccg cggtggttcc
     6541 gacgatggcg gtcgttcccc gcgtgccccg aaacgacggg tgaaactcgc ggcgcaccat
     6601 ggttcggcgt gcccgaagcg cctgcaatga tttgggccat ggctctgcaa cacaacacgt
     6661 acttcgacgg aaccgtgcaa agcatcggat tcgtcacggg tgagggacgc tcgagcgtcg
     6721 gggtgatgga cgttggcgag taccacttcg gcaccgaggc ccccgagcgg atgaccgtgg
     6781 tggcgggcga gctgatcgtg cagcttccgg gcgcgccgtg ggcgacctac gtagcgggct
     6841 cttctttcga ggttccgggc aacagcgcct tcgacctcaa ggtcaagcgg ccgacggcct
     6901 atctctgcga ctacctgtga cggttccgtc gacgccggaa ctcacccggg tttcacgtgg
     6961 atggaccgct cgtttcggtc tcatctggct gggtgtgtgg atcgcgatgc tggtccccgt
     7021 ctcgctcgcg ctgccggatc agctcgcgac ggtcgaccac gctcaccggt atcgcaactt
     7081 cggtctgatc aacgcccttg tcgggctggc ggcgctcgtg agcctgccgt tgttcggggc
     7141 gatgtgtgac cggacgaact ctcgattcgg gcaccgcaag gcgtgggttc tgggcggcgt
     7201 cgtcgcgatg gtcgtcgggt tgattctgac cggccggcaa gacacctggc agatgatcgc
     7261 gttctggtgg atcgtggcga atctgggcaa ctccgcgatc gcgacggggc tgaccgcctc
     7321 ggttgccgac caggttcccg acgatcaacg cggcacgatc tcggggttca tgtacggcac
     7381 tcagggcctg ggcatcgtcg tcggaatggt ggcggtcgag gggttcgagg ccgccacccg
     7441 ctacgtgctg ttggccatcg cgctcgtgtt gtgcgccctg ccgtatctgg tggtgaaccg
     7501 cgacgtcgaa ccgagcgccg gcaccgaacc gctcaccctc aagcggatcc tgtcgaacat
     7561 gtggatctcg ccgcgtcgct atcccgactt cggatgggcc ttcggctcgc ggctgttggt
     7621 caacgtgggt aacgccctcg gcacgacgta tctgtggttc ttccttcgcg acggcctcaa
     7681 ggtggaagac cccgacggca ccctgatcac cgtcacggtc atctacctcg tcgccacgct
     7741 gatcgcgacc ttcgtcgccg ggccggtgtc ggaccgcacc ggacgtcggc ggatcttcgt
     7801 cggcgttgcc gcgggccttc aggcggtcgc cggggtgttg gtcgccgccg caccgtcggt
     7861 cgggatgttg atggtggcgg cagccttcat cggcgccgga tacggggcct tcgtggcggt
     7921 cgaccaggcg ctcgtgaccg cggtgttgcc caacgccgat gaccgtgcca aggacctcgg
     7981 catcctcaac gtcggggccg cggttccaca aggcctcggt ccgctggcgg cggcgggaat
     8041 caccacggcg ttcgggttct cgccgctgtt cttggcggcc gggctgtcga cggcggtcgg
     8101 tgcgctcatg gtgagccggg tgcgctcggt gcgctgaggt ccgcgccgaa ggaaggtccg
     8161 cgtcgaagga aggtcactcg cgattgcggg tcggggccca gagccgttca tcaccgctgg
     8221 cgacggtccg tgcgagtacg aacaggacgt cggacaggcg gttgagatac ctgccggctt
     8281 cgccgatgtc gccgccgagt gccaacactc gccgctcggc gcgacgacaa accgtgcggg
     8341 cgaagtgcag ctgcccggcg ccattgggtc cgcccggaag gatgaagctg cgcaggggac
     8401 tcagcggcgc gttgaacgca gcggtcacgt tctcgagcca caccacggtg tcgacggtga
     8461 tgagccgtcc gcgttcgccg ggctcgggca gggcgatgtc ggcaccgaga tcgaacagtt
     8521 gctggaggag ttcttcgagc acttctgcga tcgtgacgtt cagcgcggaa ccgggctcgg
     8581 tgtcggcatc gggcccggtg tcgctgtcgg cgtcgggcaa ctcggcgagt gcgacggcgt
     8641 cggccgcgag tgccacgacc gcgttgagct cgtcgatcgc tccgatggcc tcgatgcggg
     8701 catcggtctt ggaaacccgg gtggcgtcgc ccagcgacgt ttcgccgtgg tcgcctccgc
     8761 gagtggtcac gcgggtgata tgtacggtca cggtcactcc agtcgtcgag gtgtcgtggc
     8821 accgcagtgt cgccaccccc tctgcgtcga tgttatggtc aggactcggc gcagcgcttt
     8881 cgcgaggtcg cgccgatcac aactcagctc cgcgaacggg gaaagccggt gagaaaccgg
     8941 cgctgtcccg caaccgtgag cggttccgtc cggaaccgta agtcggatcg cccgccgccg
     9001 gagatcgctt caaccaccgt cgaggaaagc gggggtcgtc gctcggcggt gagcattctc
     9061 cccgggtctc ggcccggagg aaatcatgaa tactcaatca gtcatcgccc gcccgcgccg
     9121 cacccgaacg ctgcgccttg ccctcgcgtt tgtggcgttg gtggggatcg tcacctcggc
     9181 gtgcgtgtcg gagtcgaccc cgagccagcc ggtcccggtg gcgaatatcg tcggcgagcc
     9241 atgcatgcca ggcgtgggag tgaccgtcgt ggtcgacttc caggcgctcg acgatcgcat
     9301 ttcgatcggc tgcgctgtcg ggcctcaaac cgatgggctc gctgcgttga ccaatgctgg
     9361 gttcaccatc aacaacaagc ccggcgcacc cgcctccacg atctgtcaga tcaacaatca
     9421 acccaccgcg ggctatccgg tgtgctggct gcagaactac tggagctatt ggaaatcgga
     9481 cggctccaag gcgtggagct tcagcgaggt tggcgtcgcg gcggggccgt tgccgatcga
     9541 ttccttcgag gggtggtcgt actcgccctt ctccgagggc gatatcaccg gcctcaaact
     9601 gcgccgtacg gtgaaccaga tcaaggcgtt gcggggctga ctccggtcgg tcctgcaggg
     9661 tccggtcggc gtctcggctg agcagccggt cggtactgac ccgtccggac ccaaagatca
     9721 gtggtgctat cgtgccccac tgcatcggcg aacgtcgtcg atgacaacac ttcccgcagc
     9781 gggggaagcc ggtcgaaatc cggcgctgtc ccgcagccgt gggtgagtca ccagccggtg
     9841 tctcacgagt cggaatgccc gctcgggagg gtactgaacc atccgtcgag gactgcggag
     9901 aaggctcgga ttcgtcaggt gtcgggcccc gtgccctcga agatcaccgg tcggagacga
     9961 actcttcgga ggactcgtca acgaaaggtg atttcatggc ttctgagaag ctgacccaca
    10021 cgacggtgcc tcggtcgaag gcgctcgtcc tcatcccggc aattgtcgcg gtctccctcc
    10081 tcggggcgtg caccccgaac gggcccggaa cccccgcgac cacgaccacg tccagcacca
    10141 gtacgacgac caccacggct tcgccccttc cggtctgtga ggtgcccgag gcccccgtgc
    10201 tcactctgat ccggtcgaac gaggtcctga ccttcacgat cgcggacgga tcgccgatcg
    10261 agttggcggt gatcgcctcc gacgccgatc ccggcgatgc gctctacgaa gaggcctcga
    10321 cgctgagcct tgccgcctac gagggacagg tcgtgcgcgt gctcgcccgt tccggtggcg
    10381 actgtgatcc ggaggtgatc ttcgatgcca cctacgacgt gcgggcccag atgccgaaac
    10441 gtccaggtgt cgccgggacc gatt
//
LOCUS       DAOOMA010000020        10454 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_7250_length_10454_cov_3.800750, whole genome shotgun sequence.
ACCESSION   DAOOMA010000020 DAOOMA010000000
VERSION     DAOOMA010000020.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 10454)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10454)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10454
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7250_length_10454_cov_3.800750"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            186..701
                     /gene="moaC"
                     /locus_tag="PLP95_01230"
     CDS             186..701
                     /gene="moaC"
                     /locus_tag="PLP95_01230"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015799245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HPB44454.1"
                     /translation="MPERGLTHLDPLGRARMVDVAPKDPTHRRAIARGRVNMSVETTS
                     LVARGAISKGDVLSVARVAGIQAAKRASDLIPLCHPLLVGAVLVNFRIEDTFIEVEVS
                     VDTVDRTGVEMEALSACSIACLTIYDMCKSVDRSMVIGDIALWEKTGGRSGSYRREGS
                     PFDDDDRPHGI"
     gene            964..1455
                     /locus_tag="PLP95_01235"
     CDS             964..1455
                     /locus_tag="PLP95_01235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB44455.1"
                     /translation="MLFGLGVAWAAVLAPDVLRRMSTTRRHDPMSQFSSASRNLSNVH
                     QLNTRDRRGTIDLRPNVVQAAPRRVSPQPGRKSAQQQRRQDVLVGLVAAAVLSFLAAI
                     SMGGIAMALHFVVDIALVAYIAALASVTKRERVRGPVVGYSNPALQRGVAAAYDDRRR
                     IAR"
     gene            1516..1592
                     /locus_tag="PLP95_01240"
     tRNA            1516..1592
                     /locus_tag="PLP95_01240"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1550..1552,aa:Ala,seq:cgc)
     gene            complement(1662..2477)
                     /locus_tag="PLP95_01245"
     CDS             complement(1662..2477)
                     /locus_tag="PLP95_01245"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: protein motif:HMM:NF013632.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A24 family peptidase"
                     /protein_id="HPB44456.1"
                     /translation="MSALGSLTAPLAATTGIDAFSTIALIPVVVWAFIAGTTATWIAH
                     RYREAADSDGYEFAELAAATCERCDHTVSLAETIPGRLGICGGCGRALPATWIGGVLA
                     SIAGCLAMLATFGARAIVVPYLWLIPVLVTAALVDLRTMLIPKRVVWIGVGVGLGLMT
                     ATAAWMGIISSLTSALIGAGAYFAVMFVLHVVNPNGLGFGDVRLAVLLGLYLGWIDIR
                     LTLIGLFIGNVSYLAYALPQRIRHGKDAGRFSPFGPGLAVGTIVAVCCYSLVV"
     gene            complement(2517..2993)
                     /locus_tag="PLP95_01250"
     CDS             complement(2517..2993)
                     /locus_tag="PLP95_01250"
                     /inference="COORDINATES: protein motif:HMM:NF025447.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4262 domain-containing protein"
                     /protein_id="HPB44457.1"
                     /translation="MTDDVFPDRNMEHLDKISWVIETNGWCAEPVAASADPPAPGYTY
                     TIGFEPSYGASEVVIFGLQPVAARGLLELIHAQISNGLTIPDGIFVGLLDNDLRAAML
                     PLDLDEVADLFPDATALLGADGYRMSQFVWPDRNGLLPWEDGYDERLRLAQPVLAA"
     gene            complement(3018..4451)
                     /locus_tag="PLP95_01255"
     CDS             complement(3018..4451)
                     /locus_tag="PLP95_01255"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HPB44458.1"
                     /translation="MFGEIIAEAAVRFGDREALIEDTGRTLTYAQLHHRSDAIAHSLR
                     RAGVGEGSVVAITHGSGIDYLLAFAAAAKLGATTAGINPSLAEPERERLLRQLQPDLV
                     VAAPIDLGGSVTPRLEPVDDDPERVVAIVFTSGTTGLPKGAVFRNRQLESIMACDLGN
                     DWRERWDGGGHMLVSTQMAHVGFMTKIPWYLRTGATMHVLAKWRPEPVLRLIDKYRIR
                     SLGVVAPQLALLLRHPALADIDVSCVDTIIAGGAASPSALVREATERFGAKYSIRYSS
                     TESGGIGLATAFDASAEEMFGTVGRARPGVQVRVIGDDHLPVPTGTVGQLCIKTPTGF
                     DRYWRNPEATASTLIDGWIHTGDLASVDDSGLVRLSGRLKEMYIRGGYNVAPAEVEAV
                     LCDHPLVAEIAIAPRTDPMMGEIGVAIVRPSDPAHPPTLETLREFGASELAAWKLPEA
                     LVITDSLPLTAMQKLDRTELARLAATR"
     gene            complement(4496..5116)
                     /locus_tag="PLP95_01260"
     CDS             complement(4496..5116)
                     /locus_tag="PLP95_01260"
                     /inference="COORDINATES: protein motif:HMM:NF017532.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phytanoyl-CoA dioxygenase family protein"
                     /protein_id="HPB44459.1"
                     /translation="MSGTIQTGPLSARFNHDGYERLDDVFDLESIAELRADFPGQLMR
                     TSKLVRFSRHLMLASLARQALGPESRVINATYFEVAQGNGVGPRYRDDQYFELDAGQL
                     LTCIVALDDVTDSSGAFVVAPGGGDATALTVAMRAGSVLMCADSLTRYTNPNRNDEAA
                     VYAVFQYCSAVDLPTPLPGFAHGFATLLPSTDRVSVVANGRFVAFP"
     gene            5192..6004
                     /locus_tag="PLP95_01265"
     CDS             5192..6004
                     /locus_tag="PLP95_01265"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HPB44460.1"
                     /translation="MSERERLLRLDGRTAVITGAAQGIGAATAAGFADLGANLVLVDR
                     SGEVLDATATLLDRRGAAVASIVGDVRDTAVVEATVQTAVERFGGIDVLVNNAGGGFH
                     APFAGISAKGEAALIAENFTQVTDFIRRSLPHVPESGASIINVTSIEAHRAGPGFSIY
                     SAMKAALENLTRTLAVELAPRGIRVNCIAPDMIPTPGDAGLSEESAAMSDGRYYPTPL
                     GTMGSAEHAANVAAFLASELSAFVTGTTIHLDGGNLASSGWRRRRSDDAWVL"
     gene            complement(6030..7235)
                     /locus_tag="PLP95_01270"
     CDS             complement(6030..7235)
                     /locus_tag="PLP95_01270"
                     /inference="COORDINATES: protein motif:HMM:NF027980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylinositol-specific phospholipase
                     C1-like protein"
                     /protein_id="HPB44461.1"
                     /translation="MQRPARLTRLRGIAAALAAALVVAGCSDGSGTNAERASSTAAAS
                     TTTAPDPVDALRLNELQYIGTHNSYHISPNTSAAAATPDEQAGEQASAVAGVPMLDYT
                     HDPLPQQLATGTRAFELDVFADPDGNLFDHPMAPTLLGEGDPGLPASMSQPGFKVLHL
                     PDIDFRSTCWTFVECLTEVHEFSDANPDHVPIFIQIELKDDTLPAPLDFTATLPIGAT
                     ELDALDAEIRSVFDEEHLITPDTIRGDHPTLAEAIATDGWPTVGESRGKVVFFLDNGG
                     SVHDTYLAGSPNLQGRAAFTSAADPGDPDRAIVKLNDPFETSEIADAIAEGLIVRTRA
                     DADLEQAPSNDVTMREAALTSGAQIVSTDFPATKVAASGYVVGFGTGLQVRCNAVVVT
                     ACPTTPVTG"
     gene            complement(7276..7770)
                     /locus_tag="PLP95_01275"
     CDS             complement(7276..7770)
                     /locus_tag="PLP95_01275"
                     /inference="COORDINATES: protein motif:HMM:NF022161.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2505 domain-containing protein"
                     /protein_id="HPB44462.1"
                     /translation="MRIERQHTFSAPAPKCWEMFHDPAAHVAKFEGMGHHGVTVVEQD
                     ATDTSLSITITREVDVDGIPGFAKKFLSPRNTVVSVDTWTDNGDGTYSGQYTLDTKGT
                     PIEISGNTRLDPDGDQTIYSVEVDIRVKVPLVGSKLENFAKGIALKQLDQEFELADAW
                     LESH"
     gene            7880..9112
                     /locus_tag="PLP95_01280"
     CDS             7880..9112
                     /locus_tag="PLP95_01280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA-transferase family protein"
                     /protein_id="HPB44463.1"
                     /translation="MAKPAPLDGVRIIECSILGPGALTTHLVDLGADVIKVESPSGDY
                     IRRMTWPIIEGVSLMHLHISRGKRSIVLDLRTEEGVELFKELVKDADAVIEGMRPGGL
                     ARRGLGYEDLREINPKIVFCTISGYGMTGPYAKMPSHGIAYDTWAGLVNVESDDEGFC
                     YIPEHASVGIHAGPLFGAFGVVSAILGARATGEGCALEIAQSDASAAMDWLRSETWKA
                     YERPEDEVTGNPSDNYERRAPGTAGMRDGVRYQFYPTSDGRHVLFMASEQEFWKNFCE
                     GVGRPELFERWPGSQYGDHAKGNRELQAILNEVFLTKTAAEWILFGDEHNTPIAPVNT
                     PKTIAEDPQFLDRFEWMPADEVGADMIGTPLKFMDVDMPKMTKAPTVGQHTDAVLNDV
                     LGYDAAKIAELRANGTVG"
     gene            9141..>10454
                     /locus_tag="PLP95_01285"
     CDS             9141..>10454
                     /locus_tag="PLP95_01285"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HPB44464.1"
                     /translation="MTTTELPEDLVGATLTVPGLLDHAVERFGDREALVDRSVDPPVR
                     LGWREYRDRARAAAQGFIAAGVQPGDRVSIWAPNTWEWPVVLVGLQMAGAAVVPLNTR
                     YKGGEAAYILNRSRATALVTVQGFLGNDYVSMLEGHDLPHLKATVILRGDVPAGTVSC
                     DDFIAAGATAEHEAELDRRLAALSPGDLSDILFTSGTTGAPKGVMCTHEQVVRAFESW
                     ASLAGLKAEDRYLCINPFFHSFGYKAGIVASLVRGAALVPQATFDVDEAIATIEAERI
                     SCLPGPPTIYQTIINHPAFERDKVASLRLAVTGASSVPVQLIEDMREILGFDTVLTAY
                     GLTETSGIVTVSRHDTPIDIIANYSGRAIPGCEIRTVDESGSDVAASEPGEVIVRGYQ
                     VMLGYFEAPEQTAETIDPDGWLHTGDIGILNPDGYLRITDRMKDMF"
BASE COUNT         1825 a         3186 c         3615 g         1828 t
ORIGIN      
        1 gaacaagatc gacttcctgc gggcgacggt cgaactggcg cttgaacgcg atgatctgag
       61 cggagagttt cgcgaggttc tcggagagat cgtctcccgg tacggcatcg gctgaccagc
      121 ggaatcggtg aatgccggcg atctcgacat cttcagtgaa tcgaatcgac cggtagtgtg
      181 agcccatgcc agaacgggga ctgacccatc tcgacccact tggccgtgcg cgcatggtcg
      241 atgtggcccc caaagatccg actcaccggc gagcgattgc ccgcggtcgg gtgaacatgt
      301 cggtggagac cacctccctt gtcgctcgcg gcgcgatctc gaagggagac gttctgtcgg
      361 tggcgcgggt tgcgggaatt caggccgcga aacgcgcttc ggacctcatc ccgttgtgtc
      421 acccgctgct ggtaggtgcc gtgctggtaa acttccggat cgaggacacg ttcatcgagg
      481 tggaggtctc ggtcgacacc gtcgaccgaa ccggggtcga gatggaggcc ctgagcgcat
      541 gttcgattgc ctgcctgacg atctacgaca tgtgcaagtc cgtcgaccga tcgatggtga
      601 tcggcgacat cgcgttgtgg gagaagaccg gcgggcgctc ggggagttac cgccgtgagg
      661 ggtcaccttt cgacgatgac gaccgcccac atggcatctg agagcgcttc atccgaattg
      721 tgatctctcg tggcgtcttg tcacggattc gttacaaatc cgtcacactg ggtggaccgc
      781 ctgggacgag atcgctatgt ttggggtatc ccgctggtgt cgtttacgga tgaaggcacc
      841 tcaggtccgt agtaccccac gtctcggtcg tcatcgatcc ttcacatttc gaagctgcgg
      901 tttcggtgat ggagcggcga acgggcgaaa ggatgagtga acccaaccgt gtcgacttgg
      961 ctgttgttgt ttggacttgg cgttgcatgg gcggccgtct tggctcccga cgtacttcgt
     1021 cgcatgtcca ccacgcgccg tcacgatccg atgagtcagt tctccagcgc ctcgcggaat
     1081 ctgtcgaacg tccaccagct caacacccga gatcggcgtg gcacgatcga tcttcgcccg
     1141 aacgtcgtgc aggcggcacc tcggagggtc tcacctcagc cgggtcgaaa gtctgcacaa
     1201 cagcagcgcc gccaagacgt tctcgtcggg ttggtcgcgg cggcggtgct gagtttcctc
     1261 gccgcaatta gcatgggtgg aatcgcgatg gcgttgcatt tcgtggtcga catcgcgctg
     1321 gtcgcctaca tcgcggcgtt ggcctcggtc acgaagcggg agcgggtgcg cgggccggtg
     1381 gtcgggtact cgaaccctgc gttgcagcgc ggggtcgcag cggcctatga cgatcggcgc
     1441 cgtatcgccc gctgaggctg agacgatctc gattcgcacg ggaattccag cctcggctat
     1501 cgttctcgtg cccctggggg tgtagctcag ctggctagag cgcctcggtc gcatcgagga
     1561 ggtcgtgggt tcgactccca tcacctccac caggacgaac aaaccccgga ccgatggtcc
     1621 ggggtttgtg cattgcgggc gtgatttcac gacggcgaca ctcagaccac cagcgagtag
     1681 caacacaccg ccacgatcgt gccgacggcg aggcccggac cgaatgggga gaaccgtccg
     1741 gcgtctttac cgtggcggat ccgttggggg agtgcgtagg ccaggtagga cacattgccg
     1801 atgaacaggc cgataagggt gagacggatg tcgatccagc cgaggtagag gcccaacaac
     1861 acggcgaggc ggacatcgcc gaagccgagc ccgttcgggt tgaccacgtg gagcacgaac
     1921 atgacggcga agtaggcgcc agcaccgatc aacgcgctgg tgaggctgga gatgatgccc
     1981 atccaagcgg cggtcgccgt catcagcccc aatccgaccc cgacgccgat ccacacgact
     2041 cgcttgggga tcaacatggt tcgaagatca accaaggctg cggtgaccag caccgggatc
     2101 agccacaggt acggcacgac gatcgcccga gctccgaacg tggccaacat cgcgaggcag
     2161 ccggcgatgc ttgcgagcac accgccgatc cacgtcgccg gcaacgctcg gccacagccg
     2221 ccgcagattc cgagacgacc gggaatcgtt tcggcgaggg acaccgtgtg gtcgcatcgc
     2281 tcacacgtcg ccgccgcgag ttcggcgaac tcgtaaccgt cggaatcggc ggcttcgcga
     2341 taccggtggg cgatccaggt tgcggtggtc ccggcgatga acgcccacac gacgacgggg
     2401 atcagggcga tcgtcgagaa cgcatcgatt ccagtggtcg cggcgagggg cgcggtgaga
     2461 gatccgagag cagacacgac tctctatcgg cacggatcgg ccgcggatga aggcaatcag
     2521 gcggcgagta ccggttgggc gaggcgaagg cgctcgtcat agccgtcttc ccacggcagc
     2581 aacccgttgc gatccggcca cacgaactgc gacatgcgat agccgtcggc tccgagcaag
     2641 gcggtggcat cgggaaacag gtcggcgacc tcatcgaggt cgagtggcaa catcgccgcg
     2701 cgaaggtcgt tgtcgagcag gccgacgaag atgccatccg gaatcgtgag cccgttgctg
     2761 atctgggcat ggatcagctc gagcaggcca cgcgccgcca ccggctggag cccgaagatc
     2821 acgacctccg aggcaccgta ggagggttcg aatccgatcg tgtaggtgta gccgggtgcg
     2881 ggcgggtccg cgctcgcagc gacgggttca gcacaccacc cgttggtctc gatcacccag
     2941 gagatcttgt cgaggtgctc catgttgcgg tcgggaaaga cgtcatcggt cattgccggc
     3001 aacctactgc ccgggcgcta tcgggtggcg gcgaggcgcg ccagttcggt gcgatcgagt
     3061 ttctgcatgg ctgtgagcgg gagcgaatcg gtgatcacca gtgcctcggg cagcttccac
     3121 gcagcgagtt cggatgcacc gaactcgcgc agcgtctcca gcgtcggtgg gtgggccgga
     3181 tcgctcggcc gcacgatggc gacgccgatc tcgcccatca tggggtcggt tcggggtgcg
     3241 atcgcgatct ccgcgacgag cgggtggtcg cacaagacgg cctccacctc cgcgggggcc
     3301 acgttgtagc cgccgcggat gtacatctcc ttcaggcgtc ccgacaggcg aacgagccct
     3361 gagtcatcga ccgaggcgag gtcgccggtg tgaatccagc catcgatcag tgtggatgcc
     3421 gtcgcctcgg ggttgcgcca gtatcggtcg aacccggtcg gggtcttgat gcagagttga
     3481 ccgacggtcc cggtgggcac ggggaggtgg tcgtcgccga tcacccggac ctggactccg
     3541 ggccgggcgc gaccgacggt gccgaacatc tcctccgcgg acgcgtcgaa tgccgtggcg
     3601 agaccgatcc ctcccgattc ggtcgaggag tagcggatcg agtacttggc gccgaagcgt
     3661 tcggtggcct cgcgtacaag ggccgagggg gaggcggccc cgccggcgat gatcgtgtcg
     3721 acacaggaga cgtcgatgtc ggcgagcgcc gggtggcgca acagcaacgc caactgaggc
     3781 gccaccacgc cgaggctccg aatccggtac ttgtcgatca ggcgaagcac cggttcggga
     3841 cgccacttcg cgagcacgtg catggtcgcg ccggtgcgca gataccacgg aatcttggtc
     3901 atgaatccga catgagccat ctgggtcgat accaacatgt gtccgccgcc gtcccagcgt
     3961 tcacgccagt cgttacccag gtcgcacgcc atgatcgact cgagctggcg gttgcgaaac
     4021 actgccccct tgggcaagcc ggtggttcct gaggtgaaga cgatggccac cacacgctcg
     4081 gggtcgtcgt cgaccggctc cagccgggga gtgacggaac cgcccaggtc gatcggcgcg
     4141 gcgaccacca ggtccggctg gagctgcctc agcaaccgtt cgcgctccgg ttcggcgagc
     4201 gacggattga tccccgcggt agtggcgccg agcttcgccg cggccgcgaa cgcgagcagg
     4261 tagtcgatcc ccgacccgtg ggtgatggcg accaccgacc cctcgcccac gccggcccgg
     4321 cgcagcgagt gtgcgatcgc atcggatcgg tgatgcaact gtgcgtacgt cagcgttcgg
     4381 cccgtgtcct cgatgagggc ctcacggtcg ccgaatcgca ctgcagcctc agcgatgatc
     4441 tcaccgaaca cgaccgacat tgttgtcgtc ggggccggtc gaggcgagcc ggtgctcagg
     4501 ggaacgcgac gaagcggccg ttggcgacga cgctgacccg atcggtcgac ggcagcaagg
     4561 tggcgaagcc gtgtgcgaac ccggggagtg gcgtgggaag gtcgacggcg ctgcagtact
     4621 gaaagaccgc gtacaccgcg gcctcgtcgt ttcgattcgg gttggtgtaa cgggtgagcg
     4681 agtcggcgca catcagaacg gaaccggccc gcatggctac ggtgagcgcg gtcgcgtcac
     4741 ccccgccggg ggcaacgacg aatgcccccg aggagtcggt cacgtcgtcg agcgcgacga
     4801 tgcaggtcag cagttggcct gcatcgagtt cgaagtactg gtcgtcgcgg taacgcggtc
     4861 ccacaccgtt gccctgcgcg acttcgaagt aggtggcatt gatgacccgc gactcgggtc
     4921 cgagggcctg tcgtgccagg ctggccaaca tcaggtggcg gctgaagcgc accagtttgg
     4981 aagtgcgcat gagttggccc ggaaaatccg ctcgtaactc tgcgatggac tcgaggtcga
     5041 acacatcgtc caatcgctcg tagccgtcgt ggttgaaccg ggcggagagt ggcccggtct
     5101 ggatggtccc gctcatcgac atcggctatc acgatagggc aataggcaat tatccggtag
     5161 ttcgggatgg atcctcgata atgatgtcgt gatgtctgaa cgcgaacgac tcctgaggct
     5221 cgacggccgg accgcggtca tcacgggtgc tgcgcaggga atcggcgccg ccaccgccgc
     5281 cggtttcgcc gatctgggcg ccaacctcgt gctggtggac cgttccggcg aggtgctcga
     5341 cgcgaccgcg acgctgctcg atcgtcgagg cgccgcggtg gcgtcgatcg tgggcgatgt
     5401 tcgcgatacc gccgtggtcg aggcgacggt tcaaaccgcg gtcgagcgct ttggtggtat
     5461 cgacgtgctg gtcaacaacg ccggtggcgg gttccacgca ccctttgccg gaatcagcgc
     5521 gaagggcgag gcggcgctca tcgcagagaa tttcacccag gtcaccgact tcatccggcg
     5581 aagcctgccc cacgtgccag agtcgggggc ctcgatcatt aatgtcacct cgatcgaggc
     5641 gcaccgggcc ggaccgggat tctccatcta ttcggcgatg aaggccgcac tggagaacct
     5701 caccaggacc ttggcggtcg agttggcgcc ccgcggtatc cgcgtgaact gcatcgctcc
     5761 cgacatgatc ccgacccccg gagacgcggg attgagcgag gagtcggcag cgatgagcga
     5821 tgggcgctac tacccgacgc ccctggggac gatggggtcg gccgagcacg ctgccaacgt
     5881 cgcagcgttc ctggcgagtg agttgagcgc cttcgtgacc ggaacgacga ttcatctcga
     5941 cggcggcaac ctcgcgtcgt cgggatggcg gcggcgtcgc agcgacgacg cctgggtcct
     6001 gtagcgccta ggcttcggcc cgaaccggat cagccggtga ccggagtggt cgggcaggcc
     6061 gtgaccacga ccgcgttgca acggacctga aggccggttc caaagccgac cacatacccc
     6121 gaggccgcga ccttggtggc agggaaatcg gtggagacga tctgggcgcc ggaggtcaac
     6181 gcggcttcgc gcatcgtgac gtcattggag ggagcctgtt cgagatcggc gtcggctcgg
     6241 gtgcgcacga tgagtccctc ggcgatcgca tcagcgatct ccgaggtctc gaaggggtcg
     6301 ttgagcttca cgatggcccg gtccgggtcg ccgggatccg ctgcgctcgt gaaggcggca
     6361 cgtccttgca ggttcggcga tccggcgagg taggtgtcgt gaaccgaacc tccgttgtcg
     6421 aggaagaaca cgaccttgcc gcgactttcg ccgaccgtgg gccatccgtc ggtggcgatc
     6481 gcctccgcga gcgtggggtg atcgccgcga atcgtgtcgg gggtgatgag gtgttcctcg
     6541 tcgaagacgg agcggatttc agcgtcgagc gcgtcgagtt cggtggcgcc gatgggcagg
     6601 gtggcggtga agtcgagcgg cgccggcaac gtgtcgtctt tgagctcgat ctggatgaag
     6661 atcggcacgt ggtcggggtt ggcatcggag aactcgtgaa cctcggtgag gcactcgacg
     6721 aaggtccaac acgtcgaacg gaagtcgatg tcgggaaggt gcaacacctt gaacccgggc
     6781 tggctcatcg aagctgggag gcccgggtcg ccttctccga gcagcgtcgg agccatcggg
     6841 tgatcgaaca ggttgccgtc ggggtcggcg aagacgtcga gttcgaaggc ccgggttccg
     6901 gtcgcgagtt gctgggggag cgggtcgtgg gtgtagtcga gcatcgggac cccggccacc
     6961 gcagaggcct gctctcccgc ctgttcgtca ggggttgctg cggctgcgga ggtgttcggg
     7021 gagatgtggt agctgttgtg ggttccgatg tactgcagtt cgttgaggcg cagggcgtcg
     7081 accgggtcgg gcgcggtggt cgtcgaagcc gcggcggtcg aggaggcccg ctcggcattc
     7141 gtgcccgaac cgtcgctgca tccggcgacg acgagcgcgg cggcgagcgc agcggcgatg
     7201 ccgcggagac gggtgagccg tgcgggtcgt tgcatgtcga gggtccgtcg ttcggattgg
     7261 gtcgccaggt ccgcgtcagt gggactcgag ccacgcatcg gcgagttcga attcctggtc
     7321 gagttgtttg agagcgatgc ccttggcgaa gttctccagc ttggagccga cgagtggcac
     7381 cttgacgcgg atgtcgacct caacggagta gatcgtctgg tcgccgtccg ggtcgagccg
     7441 ggtgtttccc gagatctcga tcggcgttcc cttggtgtcg agggtgtact gccccgagta
     7501 ggtgccgtcg ccgttgtcgg tccaggtgtc gacgctgacc acggtgttgc gcggactcag
     7561 gaacttcttg gcgaaccccg gaataccgtc gacatcgacc tcgcgggtga tggtgatcga
     7621 tagcgacgtg tcggtcgcgt cctgctcgac gacggtgaca ccgtggtggc ccatgccctc
     7681 gaatttcgcc acgtgtgcgg cagggtcgtg gaacatctcc caacatttgg gtgctggggc
     7741 ggaaaacgtg tgttgtcgtt cgatcctcac gaaggtagat acttgcacct tgtgtgcggc
     7801 tcgcgtcggg tgtcgcgtga ttgaggggcg gggagatcgg cggtagattc tgacgactcg
     7861 tcagatctcg gaggtccccg tggcaaagcc tgcaccgctc gacggagtgc gcatcatcga
     7921 atgttcgatt ctgggcccgg gcgccctcac gacccatctg gtcgatctcg gcgccgatgt
     7981 gatcaaggtg gagtctccat ccggcgacta catccgtcgc atgacctggc cgatcatcga
     8041 gggcgtgtcg ctgatgcacc tgcacatcag tcgtggcaag cgatcgatcg tgctcgacct
     8101 gcgcaccgag gagggtgtcg agctgttcaa ggagctcgtg aaggacgcgg atgcggtcat
     8161 cgagggcatg cgccccggcg gcctggcccg acgcggtctc ggatatgagg acctgcgcga
     8221 gatcaacccc aagatcgtgt tctgcacgat ctcggggtat ggaatgaccg gaccgtacgc
     8281 aaagatgccg agccacggca tcgcctatga cacctgggcg ggcctggtga acgtggaaag
     8341 cgacgacgag ggcttctgct acatccccga gcacgcatcg gtcggcatcc acgcaggccc
     8401 gctgttcggg gcgttcggcg tcgtgtcggc gatcctcggg gcgcgtgcca ccggcgaagg
     8461 gtgtgcgttg gagatcgcac agtcggatgc gtccgcggcg atggactggt tgcgcagcga
     8521 gacctggaag gcctacgaac ggcccgagga cgaggtcacc ggaaacccga gcgacaacta
     8581 cgagcgccgg gcaccgggaa ccgccggaat gcgcgacgga gtgcgttatc agttctaccc
     8641 gacctccgat ggccgccatg tgttgttcat ggcctccgag caggagttct ggaagaactt
     8701 ctgtgagggc gtgggtcgcc ccgagttgtt cgagcgttgg ccgggcagtc aatacggcga
     8761 tcatgccaag ggcaaccgtg aactgcaggc aatcctcaac gaggtgttcc tgaccaagac
     8821 cgcagccgaa tggatcctgt tcggcgatga acacaacacc ccgatcgccc cggtcaacac
     8881 tcccaagacg atcgccgagg acccgcagtt cctcgatcgc ttcgagtgga tgccggccga
     8941 cgaggtggga gcggacatga tcggaacccc cctcaagttc atggacgtcg acatgccgaa
     9001 gatgaccaag gccccgacgg tgggccagca caccgatgcc gtgctcaatg acgtgttggg
     9061 atacgacgcc gcgaagatcg ctgagcttcg cgccaacggc acggtcggct gagtggacga
     9121 cggcgaccga gggggacctg atgaccacca ccgaattgcc tgaggatctt gtcggcgcca
     9181 cgttgacggt gcccgggctg ttggatcacg cggtcgagcg gttcggcgat cgcgaggcgc
     9241 tggtcgaccg cagcgtcgac ccgccggtac gactgggctg gcgtgagtat cgcgatcggg
     9301 cgagggctgc ggcgcagggg ttcatcgccg ccggagtgca gccgggcgat cgcgtctcga
     9361 tctgggcgcc gaacacgtgg gaatggccgg tcgtgctggt cggcctgcag atggcgggag
     9421 ctgcggtggt tccgctcaac acccgctaca agggtgggga ggctgcctac atcttgaacc
     9481 gcagtcgcgc cacggcgttg gtgacggtgc aagggttcct ggggaacgac tacgtgtcga
     9541 tgctcgaagg ccacgacctg ccccatctga aggcgacggt cattctacgc ggagacgtcc
     9601 cggcgggaac cgtgagctgc gacgacttca tcgcggcagg cgccaccgcc gaacacgagg
     9661 ccgaactcga ccgacgtctc gccgcgctgt cgcccggaga cctgtccgac atcttgttca
     9721 cctcgggaac gacgggcgcc cccaaggggg tcatgtgcac ccatgaacag gttgtgcgcg
     9781 ccttcgagtc gtgggcgtcg ctcgcgggtc tgaaggccga ggaccggtat ctgtgcatca
     9841 acccgttctt ccactcgttc gggtacaagg cgggaatcgt ggccagcctc gttcgcggcg
     9901 ccgccctcgt tccccaggcc accttcgatg tcgacgaggc gatcgccacc atcgaggccg
     9961 aacgtatttc ctgcctcccg ggcccgccca ccatctatca gaccatcatc aaccatccag
    10021 cgttcgaacg cgacaaggtg gcgagccttc gcctggcggt caccggggcg tcgagtgttc
    10081 ccgtgcaact catcgaggac atgcgcgaga tcctcggatt cgacaccgtg ctgaccgcgt
    10141 acgggctcac cgaaacctcg ggcatcgtga cggtctcgcg ccacgacaca ccgatcgaca
    10201 tcatcgccaa ttactcgggc cgggcgattc ccggctgtga gatccgcacc gtcgatgagt
    10261 caggcagcga cgtcgccgca agcgaacccg gtgaggtgat cgtgcgtggc taccaggtca
    10321 tgctcggcta ttttgaggcc ccagaacaga ccgcagagac gatcgacccc gacggctggt
    10381 tgcacaccgg cgacatcggc atccttaacc ccgacggcta tctgcgcatc accgaccgga
    10441 tgaaggacat gttc
//
LOCUS       DAOOMA010000021        10418 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_7283_length_10418_cov_4.041301, whole genome shotgun sequence.
ACCESSION   DAOOMA010000021 DAOOMA010000000
VERSION     DAOOMA010000021.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 10418)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10418)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10418
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7283_length_10418_cov_4.041301"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..638)
                     /locus_tag="PLP95_01290"
     CDS             complement(<1..638)
                     /locus_tag="PLP95_01290"
                     /inference="COORDINATES: protein motif:HMM:NF016777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HPB44465.1"
                     /translation="MTEQTSPDPTRFGGYGGATIDTLIGFPTDPDQLYASVRQRVRDD
                     ESKTMAMPASYMFHDVPELHGDGFDPVAVTLAEMDRFGVEVGLVSLGANPEAVATALE
                     RHPDRFVASITLDPDEGTGALRRLVDAHERNNLRAVSLFPHGTASRTPIDGARMFPIY
                     AKCVELGLPVFITVGIAGPRVPSDAQKVELLDRVMYEFPELVVVMRHGAEPWV"
     gene            complement(656..1834)
                     /locus_tag="PLP95_01295"
     CDS             complement(656..1834)
                     /locus_tag="PLP95_01295"
                     /inference="COORDINATES: protein motif:HMM:NF016777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HPB44466.1"
                     /translation="MTDQPFFDFDNHYYEAPDAFTRYQDRAFRSRGVRWAEIDGRKRL
                     LVGGTLNSYVANPTFDPVAKPGSLFDWYRGNPHQQSIVEAFGELEPLPAEYHDRDARL
                     AVMDTQGVGATLMFPTLGVGIEEALRHDSEAAAAAFTAFNRWLADDWGYNYQGRIYAV
                     PYIQMLDPDAAAAELQRVIDLGAIAVNIRNAPVPVPGGYRSPFDPVYDKVWGLAAEAG
                     IVVATHAGNDGYDALIQMWEPGGAESSLFRSPLRGVVTKARAVSDFYAAALCHKVFER
                     FPTLRLASVENGASWIPELLHRLDDAANRNPGFFAEHPVEVFRRQVWVTPFWEDDVSG
                     LVDHVGVDRLLLGSDWPHAEGTRVPAEFITETLAGLDAATIERISTNNALELLGVSMP
                     "
     gene            2049..2534
                     /locus_tag="PLP95_01300"
     CDS             2049..2534
                     /locus_tag="PLP95_01300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB44467.1"
                     /translation="MSEPTAGGSANDVSAEDIREFRNAVRSVLGVGTDQLNEPDPDGL
                     ARWHELVDLGASTLCGMAEGQRQDLVNVAAAIARDFGAALSPVPFAATTAVSRMLSRV
                     LVDSTDLGDDALDSVRSVLAGIASGDTLVAYGRIDAAGVARRVHGADIASALLLEDLG
                     R"
     gene            2598..4298
                     /locus_tag="PLP95_01305"
     CDS             2598..4298
                     /locus_tag="PLP95_01305"
                     /inference="COORDINATES: protein motif:HMM:NF012656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HPB44468.1"
                     /translation="MSRSIGEVSVPDLDAGIALGSDPSVGAVAGLLLAADTVGGLERA
                     LDVTVAYAAQRIAFGKPIGAFQAVQHRLADHAVSLRAMSLLVDAAADAFASGRPEALR
                     LGLLAEVAVSDHALHLAHDLVQLTGGIGFTWEYGLHFVERRAQANNALGANPRRALTS
                     LIGLGLGDADSSLDGAEGATGDSFAAYRREVRDIISQSAHPMREEGVRVPVDDAEEAA
                     IRTWLAEMYDHGVLGGGWPTQWGGSSDHHPMHDLVAMEELIVGGAYRPLDQVMLASHA
                     VLTHGTEAQKAELLPAIRSGRHVWCQLFSEPDAGSDLASLRTKAVADGDGWLVSGQKT
                     WSTDAQWADMGVLLARTDPEAQRHAGITAFVIPMDLPGIEIRPIREITGHAEFCEVFL
                     NEVRVGPEHVLGDLNDGWRVVTAGLASERAFVGANAIQLQRLFDDLVALAVGLRYEDG
                     TAPIDQPEVRIDLARLLARVEGVKAIVRDAVQRIFDDEEHPSDGPVAKLAYTELDVAL
                     TEAALAMIATASYGDELGDLVARWHHNFMWGRALTISGGASEIMRGLIARQLLGLPRA
                     "
     gene            4361..4765
                     /locus_tag="PLP95_01310"
     CDS             4361..4765
                     /locus_tag="PLP95_01310"
                     /inference="COORDINATES: protein motif:HMM:NF024966.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HPB44469.1"
                     /translation="MTEHRHDDLENIRRLLANYNLHLDVDDVDAWVELFTDDAEYHVY
                     GRVFAGHDGIRKIPTGAPKGLHLGGQPFIELVGDDRASVRSNLLFVEAGTDVLRSVVY
                     DDDLVRTEAGWRFAVRRCRFIRTHGLDDRPER"
     gene            complement(5254..5493)
                     /locus_tag="PLP95_01315"
     CDS             complement(5254..5493)
                     /locus_tag="PLP95_01315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB44470.1"
                     /translation="MSSTERDSRDWPTAPSGARRPAAIFANPNSGDYRLTGTWTSGGG
                     TALSTAGKSALGLTDTTEASGKWPITPSLRTAQSL"
     gene            complement(5526..6743)
                     /locus_tag="PLP95_01320"
     CDS             complement(5526..6743)
                     /locus_tag="PLP95_01320"
                     /inference="COORDINATES: protein motif:HMM:NF019421.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HipA domain-containing protein"
                     /protein_id="HPB44471.1"
                     /translation="MTDHLELRHGPKVIGVLERSSGSSVTLRYSNEVLDSVETGRIVV
                     SCSAPVQQAPVNVTAWCRGLLPEGQHLHALAARADVAASDTFGLLRRYGRDIAGAFEI
                     VTVDPPPRDPDIAIYGPGELDAAILNLEDEPLGIADDSELSLAGLQDKMTAVRIEDRW
                     ARPIHGYPSTHILKVDSTTHRGIVAAEAACLRLALAVGLTTIDASVTVVGDRDVLIVP
                     RYDRSIERNGTVVRLHQEDLLQALGVNPADRRERAKYQHSSSAPSWWRCADLLDSYSD
                     NWNHEVSRLIAAMTFTTAIGNGDCHAKNVSLMINDGTVTLAPLYDTVPTMLWPALRDR
                     AALWVNDVRRNSAITGADLVEEAVRFRVPVGRAASAVEDTLEEMTANLRVLDHEEAFE
                     AITANIDRLRRSL"
     gene            complement(6736..7008)
                     /locus_tag="PLP95_01325"
     CDS             complement(6736..7008)
                     /locus_tag="PLP95_01325"
                     /inference="COORDINATES: protein motif:HMM:NF025120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HPB44472.1"
                     /translation="MVERSDPVRVVRVRSERDLGLALREARRSRGLTQADLAEQIGIE
                     RAYLAALEAGRSTRLVGHLLRSFRRLGVNVTISWDVSDKRSESSDD"
     gene            complement(7109..7384)
                     /locus_tag="PLP95_01330"
     CDS             complement(7109..7384)
                     /locus_tag="PLP95_01330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB44473.1"
                     /translation="MIGDSSLSAEECAERGGRKTGGESGWMMHAWVVPGCKSPWGVFS
                     AATPLLDSALRETSGADGGARAGVRCATATGSTTAETSSADRPFGWV"
     gene            complement(7381..7740)
                     /locus_tag="PLP95_01335"
     CDS             complement(7381..7740)
                     /locus_tag="PLP95_01335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB44474.1"
                     /translation="MTRRCRANRSPTTSTTRCDRDFNIAASRDEAEVLGVDMYQGGAM
                     PADHETTAAEEGGDGGHVGPQPWTAMTDPKQCAQLEDELAEAREVALSMPSRRRASPE
                     RTITGTATSACARESAG"
     gene            7914..8120
                     /locus_tag="PLP95_01340"
     CDS             7914..8120
                     /locus_tag="PLP95_01340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB44475.1"
                     /translation="MTTIEVFADVGCPFAHISLHRFVARRAEMGRSDVRLHVRAWPLE
                     LVNATPMDPEFIAEEIDEIRPQVA"
     gene            8130..8525
                     /locus_tag="PLP95_01345"
     CDS             8130..8525
                     /locus_tag="PLP95_01345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB44476.1"
                     /translation="MGFAAAAFPSTSLPALALEAAAYGVDSTVGEAVSLELRDRLFER
                     GENIADPAVLADIAAAHAIAYDPTDLSAPERDHREGAERGVIGSPHFFTPAGSFFCPA
                     LDIGRNDEGHLVAASDLTGFEAFLATCFT"
     gene            complement(8580..9203)
                     /locus_tag="PLP95_01350"
     CDS             complement(8580..9203)
                     /locus_tag="PLP95_01350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB44477.1"
                     /translation="MNYVNAFHHGVERSHSLPLSIRLIREMHAIILAGARGQERHPGE
                     LRTSQNWIGTGGATLAAPLLYLSPYLEAHRQAYYDALQGVRERGDLETWLQLILEGIR
                     VQSIDAATRAQELLALREHYRAIFAARNSSSVIATIDLAFQSPVLNAAMVEHALGVSR
                     PTAIAALNKLASAGMLSELPPGPRGARRWSAASVIGILAADEPGAPA"
     gene            9504..9773
                     /locus_tag="PLP95_01355"
     CDS             9504..9773
                     /locus_tag="PLP95_01355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB44478.1"
                     /translation="MAGDDVRINALLGQRTTYLRDPRRLADVGYAERVVRELSCDDLA
                     AMAGVDVDVVRRMERALPLRAPGDVRRVLRSIGIEPRALPDMAIT"
     gene            9770..>10418
                     /locus_tag="PLP95_01360"
     CDS             9770..>10418
                     /locus_tag="PLP95_01360"
                     /inference="COORDINATES: protein motif:HMM:NF025041.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HipA N-terminal domain-containing protein"
                     /protein_id="HPB44479.1"
                     /translation="MNGRIEVWREGRHVGQFVVAADGVSFDYDEGASATPISLSLPRD
                     RPAKKKAAFNFLANLLPEQERTRARMASVYGAASAGVFDLIHAAGGDMAGGLVLLPED
                     QEPTKRASEISPAADQDIAKRVAAIKRDDSDTTPAGVPARFLLAGAQGKFALSLVEDD
                     WYWPNESVPSTHIVKPGSPTYRGIEAAEAAAMNLAKRAGLSVAAAGVETFADQSAY"
BASE COUNT         1780 a         3308 c         3468 g         1862 t
ORIGIN      
        1 acccacggct cggcaccgtg tcgcatgacg acgaccaact cgggaaactc gtacatcacc
       61 cggtcgagaa gttcgacctt ctgtgcatcc gacggaaccc gcggcccggc gattccgacc
      121 gtgatgaaca ccggcaggcc gagctctacg catttggcgt agatcggaaa catccgtgca
      181 ccgtcgatgg gcgtacgcga cgccgtgccg tggggaaaca acgacaccgc tcgcaggttg
      241 tttcgctcgt gggcatccac cagacgccgg agtgcaccgg tgccttcgtc gggatcgagg
      301 gtgatcgagg cgacgaagcg gtcggggtgg cgttcgagtg cggtggcgac cgcctcgggg
      361 ttggcgccga gactgacgag gccgacctcg acgccgaaac ggtccatctc cgcgagtgtc
      421 acggcgacgg gatcgaaacc gtcgccgtgg agctcgggga cgtcgtggaa catgtagctc
      481 gccggcatcg ccatggtctt cgactcgtcg tcgcggaccc gctgacgcac cgatgcgtag
      541 agctgatcgg gatcggtggg gaacccgatc aacgtgtcga tcgtcgctcc gccatagccg
      601 ccgaaccgcg tcgggtcggg cgaggtctgc tcggtcatat gagcgccgtg cgcactcacg
      661 gcatgctcac gcccagcaac tcgagcgcgt tgttcgtcga gatgcgttcg atggtcgcgg
      721 cgtcgagccc cgccaacgtt tcggtgatga actcggcggg cacgcgggtt ccttcggcgt
      781 ggggccagtc cgaaccgagc aggagccggt cgactccgac gtggtcgact aggccgctga
      841 cgtcgtcctc ccagaacggc gtgacccaca cctgacggcg aaaaacctcg accgggtgct
      901 cggcgaagaa gccgggattg cgattcgctg cgtcgtcgag gcggtggagg agttcgggaa
      961 tccacgacgc gccgttttcg acgctcgcca gacgcaacgt cgggaatcgt tcgaacacct
     1021 tgtgacacag cgctgccgcg tagaagtcgc tgacggctcg ggccttggtg accacccctc
     1081 gtaacggcga gcgaaacagt gaactctcgg cgccccccgg ctcccacatc tggatcagcg
     1141 cgtcgtagcc gtcgttgcca gcgtgggtcg caacgacgat cccggcctct gcagccaatc
     1201 cccagacctt gtcgtagacc gggtcgaacg gcgaacggta cccgccgggc accggtactg
     1261 gggcgttgcg aatgttgacg gcgatggcgc cgaggtcgat aacacgctgc aactcggctg
     1321 ccgctgcatc ggggtcgagc atctggatat agggcaccgc gtagatgcga ccttggtagt
     1381 tgtatcccca gtcgtctgcg agccagcggt tgaacgcggt gaaggcggct gcggccgctt
     1441 cggagtcgtg gcgtagcgcc tcttcgatgc ccacgccgag ggtcgggaac atgagggtgg
     1501 cgccgacgcc ctgggtgtcc atgacggcga ggcgggcgtc gcggtcgtgg tattcggcgg
     1561 gcagcggttc gagttcgccg aacgcctcga cgatgctttg ttgatggggg ttgccccggt
     1621 accagtcgaa cagcgagccg ggcttggcga cggggtcgaa cgtggggttg gcgacatagg
     1681 agttgagcgt cccgccgacg aggagccgct tgcgcccgtc gatctcggcc caccgcacac
     1741 cccggctgcg aaacgcacgg tcctggtagc gggtgaaggc gtcgggagcc tcgtaatagt
     1801 ggttgtcgaa gtcgaagaac ggctgatccg tcatggtggt gctgctcctt cggggaggtg
     1861 gcgcgagcac ttcgtttgac gctccacgcc tccactcggc agaataccaa cattgacgcc
     1921 aaagtcaatt ttggtcgtaa tcttcagtaa tatcggggtg aacgcggccg tcaacgtggg
     1981 tttcacggct catcggtgcg tcgcgggttc gattcgaggg cgatgtgaat cttgcggagg
     2041 agatcgacgt gagcgaacca acggcaggtg ggtcggcgaa cgacgtcagc gctgaggaca
     2101 tccgcgagtt tcgcaacgcg gtgcgctcgg tgctcggcgt cggaaccgac caattgaatg
     2161 aaccggaccc ggacggcttg gcgcgctggc acgaactggt cgaccttggc gcctcgacgc
     2221 tgtgtggcat ggcggagggt cagcgccaag acctggtgaa cgttgcggcg gcgatcgccc
     2281 gcgatttcgg cgcggcgttg tcgccggtgc ccttcgctgc gacgaccgct gtctcgagaa
     2341 tgttgtctcg tgtgctggtc gactcgaccg acttgggaga cgatgcgctc gacagcgttc
     2401 ggtcggtgct cgccggcatc gcctcgggcg acacgctcgt cgcctacgga cgtatcgatg
     2461 ccgccggtgt ggcccgccgg gtgcacgggg ccgacatcgc gagcgcgttg ttgttggagg
     2521 acctcggccg gtagcgtctg gtgctcctcg atgatccggc ccagtggacc gttcgaccgg
     2581 tcgtagcgcc gttcgacgtg agccgctcga tcggcgaggt gtcggtgccc gacctcgacg
     2641 ccggcatcgc gcttggatcg gatccgtcgg tgggtgcggt ggctggcctg cttctcgctg
     2701 cggacaccgt tggcggactc gaacgagcat tggacgtcac cgtcgcgtac gccgcacagc
     2761 gcatcgcgtt tggcaaaccg atcggcgcat tccaggcggt gcaacaccgc ctcgccgacc
     2821 atgcggtcag cctgcgggcg atgagcctgc tcgtcgacgc ggctgccgac gccttcgctt
     2881 cgggccggcc cgaggcgctg cggttggggc tgctcgcaga ggtcgcggtg agcgaccacg
     2941 cgctgcatct cgcccacgac cttgtgcaac tcaccggggg aatcggcttc acctgggagt
     3001 acggactcca tttcgtcgag cgacgtgccc aggccaacaa cgcgctcgga gcgaaccctc
     3061 ggcgggcgtt gacctcgttg atcgggttgg gccttggcga cgccgatagc tcgcttgacg
     3121 gcgctgaagg cgcgaccggt gactcgttcg ccgcgtaccg gcgtgaggta cgcgacatca
     3181 tctcccagag cgcccacccg atgcgtgaag aaggggtgcg cgtgcccgtc gacgatgcgg
     3241 aggaggcggc gatccgaacc tggctcgccg aaatgtacga ccacggggtc ctcggcggcg
     3301 ggtggccgac gcagtggggt ggctcgtcgg atcatcaccc gatgcacgac ctcgtcgcca
     3361 tggaggaact gattgtcggc ggtgcgtatc gcccgctcga tcaagtgatg ttggcgagcc
     3421 atgcggtgtt gacccacggc acagaggcac agaaggccga actgctgccg gcgattcgtt
     3481 cgggtcgtca cgtgtggtgc cagttgttca gcgagcccga tgccggcagc gatctcgcct
     3541 cgcttcgcac gaaggcggtg gccgacggtg acggttggtt ggtgagcgga caaaagacgt
     3601 ggagcaccga cgcccagtgg gccgacatgg gtgtgctgtt ggcccgtacc gatccggagg
     3661 cgcagcgcca cgccggtatc accgccttcg tgattccgat ggacctgccc ggcatcgaga
     3721 tccgtccgat ccgcgagatc accggccacg ccgagttctg cgaggtgttc ttgaacgagg
     3781 tgcgcgtcgg gccggagcac gtcctcggag acctgaacga cggctggcgg gtggtcaccg
     3841 ccggattggc ctccgagcgg gcgtttgtcg gagccaacgc catccagctt caacggctct
     3901 tcgacgacct cgtggccctg gcggtcgggc ttcgctatga ggacggcact gcgccgatcg
     3961 accagccgga ggtgcgcatc gacctcgccc ggctgctcgc gcgagtcgag ggggtgaagg
     4021 cgatcgtgcg cgacgccgtg cagcgcatct tcgacgacga ggagcacccg agcgacggcc
     4081 cggtcgcgaa gctcgcgtac accgaactcg acgtcgcgct caccgaggcg gcactcgcga
     4141 tgatcgcgac ggcaagctac ggcgacgaac tcggcgatct cgtggctcgg tggcatcaca
     4201 acttcatgtg gggccgtgcg ctcacgatct ccggcggggc gtcggagatc atgcgcggcc
     4261 tcatcgctcg acagttgctc gggttgccgc gggcctgacg ctgacagacc aacgggccag
     4321 aacgaccagc gagccagaac gaccaaagga ccaacgtgtc atgaccgagc accgccacga
     4381 cgacctcgaa aacatccgcc gactcctggc gaactacaac cttcacctcg acgtcgacga
     4441 tgtcgacgcc tgggtagagc tgttcaccga cgatgccgag tatcacgtgt acgggcgggt
     4501 gtttgctggc cacgatggca tccgcaagat cccgacgggt gcgcccaagg gcctgcatct
     4561 cggcgggcag ccgttcatcg aactcgtcgg cgacgaccgg gcgagcgtgc gttcgaacct
     4621 gctgttcgtc gaggcgggca ccgacgtgtt gcgcagcgtg gtctacgacg acgatctcgt
     4681 gcgcaccgag gccggatggc gattcgccgt tcgtcgatgc cgcttcatcc gcacccacgg
     4741 cctcgacgac cgccccgaac gctgaacccg gccgtcgcgc gccgatcgga gtgattggcg
     4801 gcggtgtggc tggtggggtc tgcacgtgga gccaagcgat gccgctggta acccgaaagg
     4861 ctaccggctt cggtttgtcc ctccgggagg gtttactaca acgaagtctg ggggcgattc
     4921 gatcctgggg gatgacgcca cctcggcgat acgacacgtc actgatgatg atccgtctgg
     4981 ttggaacgct ggcctggtgc accggtctga gggttccgcg accccagacg aaccaacaac
     5041 agacgaacca acaacgagga ttactgtcaa ctacacactg tggtgggacg acaacctcaa
     5101 cggcatccgc gaccccgacg agtacccgct cgacgcaacg aattggacgt ccggcagaat
     5161 ccaggtctat gcgtacccta acgaggaggt tttcgatatc gaccgaaacg gtcatgccac
     5221 cacctcgtgg gattttcagc cgtttcctcg tgactacaac gattgcgcag tcctcaagct
     5281 gggtgtgatg ggccattttc cggaggcctc ggtggtgtcg gtgaggccga gtgccgattt
     5341 gcccgccgtg gacagtgcgg tgcctccacc ggaggtccac gtgccggtga ggcgatagtc
     5401 gccgctgttg gggttggcga agatcgctgc cggccgtcgc gctccgctgg gtgccgtggg
     5461 ccagtcccgc gagtctcgtt cagtagaact caagcgatgg cctcgggtcg ccccgcaagg
     5521 cggaatcaga gcgatcgacg cagccggtcg atgttcgccg tgatcgcctc gaacgcttct
     5581 tcgtgatcga gcacacggag attcgccgtc atctcctcca gcgtgtcttc gactgcagac
     5641 gccgcccgcc ccacaggcac acgaaaccgg acggcctcct cgacaagatc agcgccagtg
     5701 atcgccgaat tccggcggac gtcgttgacc cacagtgccg cccgatcacg cagtgcaggc
     5761 cacagcatcg tgggtaccgt gtcgtagagc ggggcaagcg tgacggtgcc gtcgttgatc
     5821 atcaggctga cgttctttgc atgacaatcg ccgttgccga tcgccgtcgt gaacgtcatc
     5881 gcagcgatga gtcgactcac ctcgtggttc cagttgtcgc tgtaggagtc caacagatcg
     5941 gcgcaacgcc accaactcgg tgcggaggat gaatgttggt acttcgccct ttctcgccga
     6001 tcagcagggt tcacgcccag tgcctgaagg agatcctctt ggtgtagccg cacgaccgtg
     6061 ccattgcgct cgatcgagcg gtcgtatctc ggcacgatca acacgtcacg gtcgcccacg
     6121 acagtcacgc tcgcatcgat cgttgtgagt ccgacggcga gcgccaggcg gaggcacgcg
     6181 gcctctgctg ccacgatccc acggtgggtc gtcgaatcca ctttcagaat gtgtgtcgag
     6241 gggtatccgt ggatcgggcg agcccatcga tcttcaatcc gaacagcggt catcttgtcc
     6301 tgcagtccag cgagcgacag ttccgaatcg tcggcgattc ccagcggttc atcctccagg
     6361 ttcaggatcg ccgcatcgag ctcgcccggg ccatagatcg caatgtccgg gtccctgggt
     6421 ggcggatcga ccgtcacgat ctcaaaggct ccggcgatgt cgcgtccata ccgtcgcagg
     6481 aggccgaagg tgtcggacgc ggcgacgtcg gctcgcgctg ccaaagcgtg caagtgctgc
     6541 ccctccggaa gtaggccgcg gcaccacgcc gtgacattga ccggagcctg ctgaaccggc
     6601 gctgagcacg acacgacgat gcggccggtt tcgacagaat cgagtacctc gttgctgtaa
     6661 cgcaacgtga ccgatgaccc cgacgaacgt tccagcacac cgatgacctt ggggccgtgt
     6721 cgcaactcaa ggtggtcagt catcgctcga ttccgaccgt ttatccgaga catcccacga
     6781 gatcgtgacg ttgacgccca accgacgaaa ggaacgcagc agatgaccca ccaggcgggt
     6841 gcttcgcccg gcctcaagcg cagcgaggta ggcgcgttcg atgccgatct gttccgccag
     6901 gtcggcctgg gtcagtcccc gcgatctgcg ggcctcacgc agtgccaagc cgaggtcgcg
     6961 ttcagagcgg accctgacca ctcgtactgg atcgctacgt tcgaccatcg gcaccccttg
     7021 atgtaccaat tccgttacat cgccagaata tcgcctcatg taccaatacc gttacatctg
     7081 ccgaattggc ttggatgcaa caatgttgtt acacccaacc gaatggtcga tcagcgctac
     7141 tcgtttcggc ggtcgtcgag cccgtagcgg tcgcgcaccg aacccctgcg cgcgcaccgc
     7201 cgtcggctcc cgaggtttca cgcaacgccg agtccaacaa cggagtcgcg gcgctgaaca
     7261 cgccccacgg gctcttgcat ccgggcacga cccacgcgtg catcatccac ccggactcgc
     7321 cgccggtctt acggcctcca cgctcggcgc actcctcagc gctcaacgaa ctgtcgccga
     7381 tcaccccgcc gactccctgg cacaggccga ggtggcggtg cccgtgatcg ttcgctccgg
     7441 tgaagccctg cgtcgactcg gcatcgacag ggcgacctcg cgggcctcgg ccagctcatc
     7501 ttcgagttga gcgcactgtt tcgggtcggt catggcggtc cagggttggg ggccgacgtg
     7561 gccgccgtcc ccaccctcct ccgccgctgt cgtttcgtga tcggctggca tcgcaccgcc
     7621 ctggtacatg tccacgccga gtacctccgc ctcgtcccgg gaggccgcaa tgttgaagtc
     7681 ccggtcacag cgggtggtgc tggtcgtcgg cgatctgttg gcgcgacagc gtcgtgtcat
     7741 ggacggtcat gtggtgagcc tctggtgttg tcggagcgcg tcacgaacct caattccgcc
     7801 gcagctgtgg tcgacagatc gagccaggta tcgaagcgca ggacccgctc gatcgaccat
     7861 accctccagg gtatacttgt gcacacggag acgcgcgagg aaacgaggtt gccatgacca
     7921 ccatcgaagt gttcgccgac gtcggctgtc cgttcgcaca catcagcctc caccggttcg
     7981 tggcgcgccg cgcggaaatg ggccgcagtg acgtgcgtct ccacgtccgg gcgtggccac
     8041 tcgagctcgt caacgccacg ccgatggacc cggagttcat cgccgaggag atcgacgaga
     8101 tccggccaca ggtcgcctag gacctgttcg tgggattcgc cgcggcggcg ttcccgtcga
     8161 cgtcgctgcc ggcgctcgcg ctcgaggccg ccgcctatgg agtcgattcg acggtcggcg
     8221 aagcggtcag ccttgagttg cgcgaccggc tcttcgaacg tggcgagaac atcgcagatc
     8281 cggctgtctt ggccgacatc gcagccgccc atgcgatcgc gtacgacccg accgacctga
     8341 gcgcgcccga acgcgatcat cgagaaggag cggagcgagg cgtcatcggc tcgccgcact
     8401 tcttcacccc ggcgggttcg ttcttctgcc cggccctcga tatcggccga aacgacgaag
     8461 gtcacctcgt ggcggcgagc gacctcacag gatttgaggc gttcctcgcg acctgcttca
     8521 cctgaggtgc tgaaccggtc gcaactcgga gttgttgcac cgactcgtac ggttgggggt
     8581 tacgccggcg caccgggttc atcggcggca aggattccga tgaccgacgc tgcactccag
     8641 cggcgagcgc cgcgtggacc gggcggcagt tcgctgagca tgcctgcgct agcgagcttg
     8701 ttcagcgcag caattgctgt cggtcgagag acccccaacg cgtgctccac catggcggcg
     8761 ttgaggaccg gagactggaa cgcaaggtcg atcgtggcga tgacgctcga cgagttcctt
     8821 gccgcgaaga tcgcgcggta gtgctcccgt agcgccaaca gttcctgagc acgggtggcc
     8881 gcgtcgattg actgaacccg aatgccttcg aggatgagct ggagccacgt ctcaaggtcg
     8941 ccccgttcgc gtacgccctg gagcgcgtcg tagtacgcct gcctgtgagc ctccaggtag
     9001 ggggagaggt agagcagcgg tgcagcaagg gttgcccctc ccgtcccgat ccagttctgc
     9061 gatgtacgca actcgccagg atgccgttct tgccctcgtg cgccggccag gatgatcgcg
     9121 tgcatctccc ggataagtcg gatgcttagc ggcagcgagt gtgaccgctc gaccccgtga
     9181 tgaaacgcgt tgacatagtt cacgacttct tagacgtcgt cgctgggtgc ggtattggtt
     9241 gcctcgacat ctcgggggat gggtgatggg aagtacgcga cgtatccgtg cgggccgctt
     9301 atgtgacgcc cgttttcgaa cagcgttggc tcgtatcgcg cgaggtccac gacgtcagcc
     9361 catcgacctt aaaggttttg ggcgctttct ttatggtcgg cctctgacct gtaagtcggc
     9421 ttaacggtcg agaagcggga ggagatcggc tcggtgtcgc ggaggtaacg aaatcgagcc
     9481 cacagcctcg gcggcccgag ggcatggccg gagacgacgt tcgcatcaat gcgttgctcg
     9541 gtcagcgcac gacctacctg cgggacccgc gtaggctggc cgacgtgggc tacgccgagc
     9601 gggtcgttcg cgagctgagc tgcgacgatc tggcggcgat ggcgggtgtg gacgtcgatg
     9661 tcgtgcgccg gatggaacgc gccctgccgc tgcgcgcacc cggtgacgtg cgacgggtac
     9721 tccgttcgat tggcatcgaa cccagagcgt tgcccgatat ggccatcaca tgaacggtcg
     9781 catcgaagtc tggcgagaag gtcgccatgt ggggcagttc gtcgtcgcag ccgacggggt
     9841 gtcgttcgac tacgacgaag gggcatcggc cacaccgatt tctctctcgt tgcctcgtga
     9901 ccggccagca aagaagaagg ccgccttcaa ctttctggca aacctgcttc cggaacagga
     9961 gcggacgcga gcgcgcatgg cctcggtcta cggcgcggcc agtgcgggcg tgttcgacct
    10021 gatccacgcc gccggaggcg atatggccgg tgggctcgtt ctcttgcccg aagaccagga
    10081 gccgaccaag cgcgcttcgg aaatcagtcc agcagcagat caagacatcg cgaaacgggt
    10141 tgcggccatc aagcgcgatg acagcgacac gacaccggcc ggcgttccag cacgattctt
    10201 gctcgccggg gctcaaggca agttcgcact ctcgctcgtg gaggacgact ggtattggcc
    10261 gaatgaatcg gtgccatcga ctcacatcgt gaagccaggg agccccacct atcgagggat
    10321 cgaggcagcg gaggcggcgg cgatgaacct ggcgaagcgg gccgggctgt cggttgccgc
    10381 cgccggtgtc gagaccttcg ccgatcaaag cgcgtatg
//
LOCUS       DAOOMA010000022        10278 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_7445_length_10278_cov_3.553654, whole genome shotgun sequence.
ACCESSION   DAOOMA010000022 DAOOMA010000000
VERSION     DAOOMA010000022.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 10278)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10278)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10278
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7445_length_10278_cov_3.553654"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(516..1373)
                     /locus_tag="PLP95_01365"
     CDS             complement(516..1373)
                     /locus_tag="PLP95_01365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB44480.1"
                     /translation="MLNLDAATVFHQWAWGGLFFLWVTTRGREVGIGYGWLLRGVYSA
                     FALISIVIGLRWNTVWVRELSSMAMIAGAGFSFVVSWQRRRAGVLHQREQEQRKSQRI
                     VEMTGIDRSEQHFDDSVPEFAPALDLIAPILGFAGLVAGGIDAGSPAWLQVARVVAGA
                     FFLGAVSDAMLLGHWYLTQPGLPRRPLIELVDWLTWVWPVHLATLVIPPGMVAVITGE
                     IDDGWNGMLGWYWAASALATIVLCRVTRLALKERYYSAVMAATGLLYLAILTGFSQDL
                     VARLVLNQA"
     gene            complement(1475..2797)
                     /locus_tag="PLP95_01370"
     CDS             complement(1475..2797)
                     /locus_tag="PLP95_01370"
                     /inference="COORDINATES: protein motif:HMM:NF022169.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2510 domain-containing protein"
                     /protein_id="HPB44481.1"
                     /translation="MAVDAVTVGAASVEAANRRPGWYRDPWRSDDLRWWDGRTWTDWV
                     HARPLAGGEQSLVPGVPVVPGVPEAPTMPEAPTTPEAPLAAEMPAAPVAPMAPATPIA
                     PIAAMPPPNVSSQVSAAGVAGPTLLPAGKIAPTAATAEMLSTEMLSTLSTLPPPPVQR
                     AEVPGEYGLEGSPLRPSGHAQAIRARTGRPSAWPTARPTVSPSVSAAGLWRFVGVAIA
                     IAVLVTRCVNAFDSATDRVDLDVSDRPVTAPTVLETGIQGELIATGCDRVLAEPTSTA
                     REMVVRLGVEWGEVFNTSMEEYASLAIDRCVAGAYGESVQPTHDGIRCSPADVSVLAA
                     GPARYVGTCASVELVVVAVIGPCEVEAVYDEVQHSTIEEFRGAPSIFDGNAFGVGCVF
                     EGLGDQQPTPGTRMSATVRVLAGRPEVIWGQQMRPSGTFEVVSFEIHR"
     gene            complement(2807..3955)
                     /locus_tag="PLP95_01375"
     CDS             complement(2807..3955)
                     /locus_tag="PLP95_01375"
                     /inference="COORDINATES: protein motif:HMM:NF012766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HPB44482.1"
                     /translation="MGEAVSSGESGSVGVAPFVARVGRVAERMRALGVDALTLSVGPD
                     LPWLVGYEAMPLERITMAVLLADGTCSLVVPELEAPRVVSRDHFSVIPWGETDDPIAL
                     IVERVGEAGTIAFGDRTWSRFLIELQDRLPNRRWLRSNEVTGPLRAVKDDHEIDMLAR
                     AGAIVDEIAVELQQGRIPLVGRTEAEVSADLSRRIIERGHGRVNFAIVAAGENAASPH
                     HEAGDRRIEAGEVVLCDFGGTLFDEWGVGYCSDITRCVWTGPVPTEAARIYDLLEAAQ
                     SAGVRAATVGASCESVDSACRDRISAGGYGEWFIHRTGHGIGVEEHEDPYMVSGNVAA
                     IEAGNAFSVEPGIYLPGRFGFRLEDIVVATGEGPRSLNNAPHGLAEVR"
     gene            complement(3948..5681)
                     /gene="lipA"
                     /locus_tag="PLP95_01380"
     CDS             complement(3948..5681)
                     /gene="lipA"
                     /locus_tag="PLP95_01380"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HPB44483.1"
                     /translation="MALHARWLGRVDYDDALGLQRALHAHTKQDYLLLLEHPHTYTMG
                     VRADAANILIDPHEVGAQLVRADRGGDVTYHGPGQLVGYPIVTLADHEAGEMPSTRDH
                     VLNLEHLLIDVLSDFGIGDASVIEGSPGVWIGAGTSRPRKIAAIGVRISRGRSMHGFA
                     LNVHPDPAMFSHIVPCGITDKGVTSMALEGVDATMREVVDAVCARAQQRWSALDRADV
                     VWRQRHDDADLSPFSRGEGPGAAVRYSSSVRQSDGTSLRLLGRLAEAGVEEQVAITEP
                     KPEWMRAPMKLDAGVLKLRSHLREQRLVTVCEEAGCPNLSECWSDGTATFMVCGERCT
                     RACGFCLVDTRKPEALDVDEPRRVAEAAAAMGLRFAVITMVARDDLPDGGAAHVAATI
                     RAIHERVPGAGVEALTSDFRGDDASLRLVFDARPEVFNHNIETVARMQRAVRPSASYA
                     RSLSVLGRGVAAGLTTKSGFVAGMGETFDEVVQTLRDLAAVGTSIVTIGQYLRPTAHH
                     LPVQRWWTPAEFAEWKRIGEEEFGISHVESSPLTRSSYHARDAAEASASGLARSAGPG
                     PVAVSIGSRHG"
     gene            complement(5687..7453)
                     /gene="sucB"
                     /locus_tag="PLP95_01385"
     CDS             complement(5687..7453)
                     /gene="sucB"
                     /locus_tag="PLP95_01385"
                     /inference="COORDINATES: protein motif:HMM:TIGR02927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase, E2 component,
                     dihydrolipoamide succinyltransferase"
                     /protein_id="HPB44484.1"
                     /translation="MAEIVMPQLGESVTEGTITKWFKQVGDSVAEDEPLFEVSTDKVD
                     SEVPSPISGTITEILVPEGETVDVGVVLALVGDGVSAEAAGTQDSAAPLAGEVPPVEV
                     PAVDVPAAEVPAVAVPAPASAPAPSVSAAPAVSGGANLDVVLPQLGESVTEGTITKWF
                     KQVGDSVAEDEPLFEVSTDKVDSEVPSPASGTLVEIRVNEGDTVDVGTVIAVLSDSVG
                     ASAAAAPAAPAPPAPASAPAPAPAPAPAPAAVVSTPVAGEPAVGAATSGRLLSPLVRR
                     LIDEHNLDPSTIAGTGVGGRITREDVLRVIDGDALRSAPETAAPATAAPTPSAPATAA
                     PTPSAPAPTPAQVPTAGAGDTVIPFNNIRRLTAEHMVRSLATSAHVITATEVDYEGVE
                     RVRKAAKEAWKAEEGFSLTYLPFITRAVVDALHEFPNLNATVGANELIVHGAVNMAYA
                     VDVNFEALLAPVVPGADGKRLRQIARDLHDVATRARSKQLSPDDLSGGTFTISNSGTF
                     GTLLVAPIINQPQVAILSTDGIKRRPVVVELPDGSESIAIHSVGNLTLSWDHRAFDGA
                     YAAAFMDRVRDIIETRDWAAEL"
     gene            complement(7674..9086)
                     /gene="lpdA"
                     /locus_tag="PLP95_01390"
     CDS             complement(7674..9086)
                     /gene="lpdA"
                     /locus_tag="PLP95_01390"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015798156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HPB44485.1"
                     /translation="MEQINPSLYDVVVIGGGPGGYATALYGASAGLKIALIEKDKLGG
                     TCLHRGCIPAKELLETAAVRRAVKHSAEFGVFSEETGIDWNVTIDRKQRVVDTMFGGL
                     KSLVKGRKIDVYEGTGLLGPSHQVNVVGSDGSQSTLLGTAIVLATGSVPRTIGGFEPD
                     GTIVFTSDEFLDLRKLPSSAAVIGGGAIGCEFASTLADMGVAVTILEGLPKILSGCDS
                     DAAKVVERAFKKKGIDIRTGVSVTGHQPRPAGGTTVTFGEGDGLDVDIVVVSIGRTPV
                     SSGVVGEGTDVAIDERGFYVVDPNCRTAEPGVWAVGDCINTPQLAHIGFIEGINVVKD
                     ILGENPAPVDYSKVPWAIYCHPEVAFAGHSEESAKAAGYEVVVSKHRFAGNGRAQILG
                     DTDGLVKVIAEKRADGTAGTILGVHMVGPWVTEQLGQGYLAVNWEATVDELAALIQPH
                     PSLSELFGETVIALTGRSLH"
     gene            9379..10113
                     /locus_tag="PLP95_01395"
     CDS             9379..10113
                     /locus_tag="PLP95_01395"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HPB44486.1"
                     /translation="MRDAVGNIASILVLGGGSEIAVETIKALTKRRLRRVVLAVRSPD
                     KVADVVDDLRSRGLEVATIPFDGADIASHDRVIASAFDDGDFDVVLSAFGQLGDQDEL
                     DDDPTAAGELAVVNYAGQVSALTAVAKQLKIQGHGTIVVLSSVAGERVRKDNATYGST
                     KAGLDGFAQGLGDRLRGTGVGVMIVRPGFVHTRMTEGMDPAPLSTTPDAVAADIVSGL
                     DRGATVIWSPLDSPMPPRRWATYPTC"
BASE COUNT         1818 a         3685 c         3218 g         1557 t
ORIGIN      
        1 aacacggggt acaacgccaa ggccccatcc ccgaagaact cgcggcagaa cccgaaccac
       61 cggcccgacc gaaggtgaaa gtccgcggcg gaaccgtcga cctcgccgac gcgatcgcca
      121 cgatccgacg ccgctaccac cgaatggccg ccacccccga cacccgcatc caccgacccc
      181 acgacgcccg caggtcaaac acaggccgag gcccgggccg gaccggacca accggaggag
      241 cgacgagggc cgggcgagcc accgccgggc aactctccct caccccagcc aaaccacccc
      301 aaccaccgat acgccaatga accggcggcc ccaatgaacc gggtccgacc gagggcggcc
      361 tcgacacagc aactgaacac agcaactgaa cacagcaact gaacacagca cccgacgaca
      421 gcgtccgcac gatcggttcg tgctgatcgt gttcccgcgc gcctgcccgt gccggtgtgc
      481 ccgtgccggt cgaatcggcc gctcggcgtt cgtgcctacg cctggttgag caccaacctt
      541 gcgacaaggt cctggctgaa gccggtgaga atcgccaggt acaacaggcc ggtcgccgcc
      601 atcaccgcgc tgtaatagcg ctccttgagt gccaggcgtg tgacgcgaca cagcacgatc
      661 gtcgcgagcg cgctcgccgc ccaataccag ccgagcatcc cgttccatcc atcgtcgatc
      721 tcgccggtga tgaccgcaac catcccgggt ggaatgacga gcgtcgccag gtgaaccggc
      781 cagacccacg tcagccagtc gacgagttcg atcagcgggc gacgcggcag accgggctgg
      841 gtcaggtacc agtgcccgag caacatcgcg tccgacaccg ctccgaggaa gaacgccccg
      901 gcgaccactc gtgccacctg aagccatgct ggcgaacccg catcgattcc accagcaacc
      961 aaacctgcga aaccaaggat cggggcgatg aggtcgagcg cgggcgcgaa ctccggcacc
     1021 gagtcgtcga agtgctgctc ggaccgatcg atcccggtca tctccacgat ccgttggctc
     1081 ttgcgctgct cctgttctcg ctgatgcaac accccggcac ggcggcgctg ccagctcacc
     1141 acaaacgaaa agccggctcc ggcaatcatc gccatcgacg agagttcgcg cacccacacg
     1201 gtgttccacc gcaacccgat gacgatcgat atcagcgcaa acgcgctgta gacgccgcgc
     1261 aacaaccatc cgtagccgat gccgacctca cggcctctgg tcgtcaccca cagaaagaac
     1321 agcccgcccc acgcccactg gtgaaacacg gttgcggcat cgagattcaa cacgtcgggg
     1381 acctcatggc agcgtcgaca ctagcgccgg actcgcaccc ggcttctctt ccgccccagc
     1441 gctgcacctc agcgatgggt ctcagcgatg gatctcagcg atggatctca aaggacacga
     1501 cctcgaaggt tccgctcggt cgcatctgtt ggccccagat gacctccggc ctccccgcca
     1561 ggaccctgac ggtcgcactc atccgtgttc cgggggtcgg ctgctgatcc cccaggccct
     1621 cgaagacaca tccgacacca aaggcgttgc cgtcgaagat ggatggcgcc ccccggaact
     1681 cttcgatggt tgagtgctgg acctcgtcgt agacagcttc gacctcgcag ggtccgatca
     1741 ccgccaccac gaccaactcc accgaggcgc aggttcccac gtaccttgcc gggccggcgg
     1801 cgagaaccga aacgtcggcc ggcgagcacc ggatgccgtc gtgtgtgggt tgcaccgatt
     1861 cgccgtacgc accggcgacg caccggtcga tcgccagcga cgcgtactcc tccatcgacg
     1921 tattgaacac ctcaccccat tcgacgccaa ggcgaaccac catctcacgg gcggttgaag
     1981 tcggctcggc caagacgcga tcgcaacccg ttgcgatcag ctcgccctga ataccggtct
     2041 ccagcaccgt tggcgcagtc accgggcgat cggagacatc caggtcgacc cgatcggtcg
     2101 ccgaatcgaa cgcgttcacg cacctcgtca ccagcactgc gatcgcgatc gcgacgccga
     2161 cgaatcgcca gagccccgcc gctgagaccg acggcgacac cgtcggcctc gccgttggcc
     2221 aggccgacgg tcgccccgtg cgggcgcgga ttgcttgcgc gtgtccgctg ggcctcaagg
     2281 gcgacccctc gagcccatac tcccccggaa cctccgctcg ctgcaccggt ggtggcggca
     2341 acgttgagag cgtcgacaac atctccgtcg acaacatctc cgcggttgcg gccgtgggag
     2401 cgatcttccc cgccgggagc agtgtcggtc cagccactcc agcggctgaa acctgcgagg
     2461 aaacgttggg tggcggcatt gccgcgatcg gggcgatcgg tgtcgccggg gccatcggag
     2521 ccaccggggc cgccggcatc tcggcagcga ggggtgcctc cggcgtggtc ggagcctcag
     2581 gcatggtcgg agcctcaggc acaccgggca caacaggaac acccggcacc agcgactgtt
     2641 cgccacccgc aagcggacgg gcgtgcaccc agtcggtcca cgttcggccg tcccaccaac
     2701 gcaggtcgtc gctacgccac gggtcgcgat accagccagg ccgccgattc gcggcctcga
     2761 cactcgccgc cccgaccgtc acggcatcga cagccacggc atcgacctaa cgaacctcgg
     2821 ctaacccgtg cggcgcgttg ttgagcgacc gagggccttc gccggtagcg acgacgatgt
     2881 cttcgagtcg aaacccgaac cgacccggaa ggtagatccc cggttcgacc gaaaaggcgt
     2941 tccccgcctc gatggcggca acgttgccgc tcaccatgta cgggtcctcg tgttcctcga
     3001 caccgatgcc gtggccggta cggtgaatga accactcgcc gtagccgcct gcgctgatcc
     3061 ggtcgcgaca cgccgagtcc accgactcac agctcgcccc caccgttgcc gcgcgaaccc
     3121 cagcgctctg cgccgcctca aggaggtcgt agatacgcgc cgcttcggtt ggaaccgggc
     3181 cggtccacac acagcgggtg atgtcggagc aatacccgac cccccactcg tcaaacagcg
     3241 tccccccgaa gtcgcacagc acgacctctc cggcctcgat ccggcggtcg cccgcttcat
     3301 ggtggggact tgcggcgttc tcaccggccg caacgatggc gaagttcacc cggccgtgac
     3361 cgcgttcgat gatccggcgc gacaggtccg cagacacttc ggcctcggtt cggcccacga
     3421 gcggaatccg cccctgctgc aactcgacgg cgatctcatc gacgatcgcc ccggcgcggg
     3481 ccaacatgtc gatctcgtgg tcgtccttca ccgcccgcag cggcccggtc acctcattgg
     3541 aacggagcca acgacggttc gggagccgat cctgcaactc gatcagaaat cgcgaccagg
     3601 tccggtcgcc gaacgcgatg gttccggctt cgccgacccg ctccacgatg agggcaatgg
     3661 ggtcgtcggt ttcgccccac gggatcacgc tgaagtggtc gcggctcacc acacgggggg
     3721 cctcgagttc ggggaccacc agcgaacagg tcccatctgc caacagcacc gccatggtga
     3781 tccgctcgag gggcatcgcc tcatagccca ccaaccaggg cagatccggg ccgaccgaca
     3841 aggtcaaggc gtcgacgccc agcgctcgca tccgctcggc gacccgacca acccgggcca
     3901 cgaacggggc gacaccgacc gaacccgatt cacccgacga caccgcctca cccatggcga
     3961 cttccgatcg agaccgccac cggcccaggg ccggccgacc gcgccaaccc cgaagcactt
     4021 gcttcggcag cgtctcgagc gtgatagctc gagcgggtga gcggcgagga ctcgacgtgg
     4081 ctgataccga actcctcctc accgatccgc ttccactcgg cgaactccgc cggtgtccac
     4141 catcgctgca ccggcaggtg gtgtgcggtc gggcgcaggt attggccgat cgtcacgatc
     4201 gaggttccga ctgcggcgag atcgcggagg gtctgaacca cctcgtcgaa ggtctcgccc
     4261 atgccggcga cgaaccccga cttggtcgtc aacccggccg cgacgcctcg tccgagcacc
     4321 gacagcgacc gggcgtagga cgcgctgggt cgcaccgcac gttgcatccg ggcgacggtc
     4381 tcgatgttgt ggttgaacac ctcagggcga gcgtcgaaaa ccagccgcag cgatgcgtcg
     4441 tcgcctcgga agtcggaggt cagggcctcg accccggcgc cgggcacccg ctcgtggatc
     4501 gcccggatcg tcgctgcgac atgggccgcg ccaccgtcgg gaaggtcatc gcgggccacc
     4561 atcgtgatca ccgcgaaacg tagacccatg gcggccgcgg cctcggcgac acgacggggt
     4621 tcgtcgacat cgagcgcctc cggtttgcgg gtatcgacca ggcagaaccc acacgctcgg
     4681 gtacaacgct ccccgcagac catgaacgtt gcggtgccgt ccgaccagca ctccgacagg
     4741 ttcgggcacc cggcctcctc acacaccgtg accaggcgct gctcgcgcag gtgggaacga
     4801 agcttcaaca ccccggcgtc gagcttcatc ggggcgcgca tccactcggg cttcggttcg
     4861 gtgatcgcca cctgctcctc gaccccggcc tcggcgaggc gccccaggag acgcaacgag
     4921 gttccgtcgg attggcggac cgaactcgag taccgaaccg cagcgcccgg cccctcgcct
     4981 cgcgagaacg gcgagagatc ggcatcgtcg tggcgctgtc gccacacgac atcggcgcga
     5041 tcgagtgccg accaccgctg ctgagcgcgt gcacacacgg cgtcgaccac ctcgcgcatc
     5101 gtcgcgtcga cgccttcgag cgccatcgac gtgacgccct tgtcggtgat cccgcacgga
     5161 acgatgtggg aaaacatcgc cgggtcgggg tgcacgttca gcgcaaaccc gtgcatggag
     5221 cgcccgcggc tgatgcggac cccgatggcg gcgatcttgc ggggccgcga cgtgccggca
     5281 ccgatccaca cccccggcga cccctcgatc accgaggcat cgccgatccc gaagtcgctc
     5341 aacacgtcga tcagcaggtg ctcaaggttc aacacgtggt cgcgggtcga cggcatctcg
     5401 ccggcctcgt gatcggccaa ggtcacaatc gggtagccga cgagctggcc cggaccgtga
     5461 taggtcacgt cgccgccgcg atcggcacgc acgagttggg caccgacttc gtgaggatcg
     5521 atgaggatgt tcgccgcatc cgcccgtacg cccatcgtgt aggtgtgcgg atgttcgagc
     5581 agcagcaggt agtcctgctt ggtgtgcgca tgcagcgccc gctggaggcc gagcgcgtcg
     5641 tcgtaatcga cccggcccaa ccagcgagcg tgcagcgcca ccggcctcag agctccgcgg
     5701 cccagtcgcg ggtctcgatg atgtcgcgga cccggtccat gaacgccgcc gcgtaggccc
     5761 cgtcgaacgc gcggtgatcc cagctgagcg tgaggttacc gaccgagtgg atggcgattg
     5821 attcgctccc atcgggcaac tcgaccacca ccggacgtcg cttgatgcca tcggttgaca
     5881 ggatcgccac ctgcggttgg ttgatgatcg gggcgacgag cagcgtgccg aaggttcccg
     5941 agttggagat cgtgaaggtg ccaccggaga ggtcgtccgg ggagagttgt ttcgaacgag
     6001 cccgggtggc gacgtcgtgg agatcgcggg cgatctgtcg caaccgctta ccgtcggccc
     6061 ccgggaccac cggggccagc agcgcctcga aattcacgtc aaccgcgtag gccatgttga
     6121 ccgcgccgtg cacgatcaac tcgttcgctc cgaccgtggc gttgaggttc gggaactcgt
     6181 gcaacgcgtc gacgaccgcc cgcgtgatga acggaagata cgtgagcgaa aaaccctcct
     6241 cggccttcca cgcttccttg gcggcctttc ggacccgctc gaccccctcg tagtcgacct
     6301 cggtcgccgt gatgacgtgc gccgaggtcg ccaggctccg caccatgtgt tctgccgtga
     6361 gtcgtcgaat gttgttgaac gggatgacgg tgtcgcccgc acctgcagtt gggacctggg
     6421 ccggcgttgg cgcgggtgct gacggcgtgg gcgcagcggt cgcgggtgct gacggcgtgg
     6481 gcgcagcggt tgcgggcgca gcggtttcgg gcgcagacct gagggcgtcg ccgtcgatga
     6541 cgcgcaggac gtcctctcgt gtgatgcgcc cgccgacgcc ggtgccggcg atcgtcgacg
     6601 gatcgaggtt gtgctcgtcg atgaggcgcc gcacgagcgg tgacaaaaga cgtcccgagg
     6661 ttgcagcccc gaccgcaggc tcgcccgcga ccggtgttga aacgacagcg gcgggagccg
     6721 gagccggcgc gggtgctgga gccggcgcgg aagccggcgc cggaggagcg ggggccgcgg
     6781 gagcggcagc cgctgatgcc ccgaccgaat ccgacagcac cgcgatgacg gttccgacat
     6841 cgaccgtgtc gccttcgttg acgcgaatct ccacgagcgt ccccgacgcc ggcgacggca
     6901 cctcggaatc gaccttgtcg gtcgacacct cgaacaacgg ctcatcctcg gcgaccgagt
     6961 ctccaacctg cttgaaccac ttcgtaatcg tgccctcggt caccgattcg ccgagctggg
     7021 gcagtaccac gtcgaggttc gctccacccg acaccgcagg cgcagcggag accgatggtg
     7081 cgggcgccga cgccggagcg ggaaccgcca cagccggaac ctcggcggcg ggaacgtcca
     7141 cagcggggac ctccaccggc gggacctcgc cagcaagcgg cgccgccgaa tcctgcgtcc
     7201 cggccgcctc ggccgaaacg ccatcgccga ccagggccag gaccacgccg acatcgacgg
     7261 tctccccctc gggtaccaga atctcggtga tggtccccga gatcggcgac gggacctcgg
     7321 agtcgacctt gtcggtcgac acctcgaaca acggctcatc ctcggcgacc gagtctccga
     7381 cctgcttgaa ccacttcgta atcgtgccct cagtcaccga ctctccgagc tggggcatca
     7441 cgatttcggc catgtcctgc ttcccttctg tgtgagagcg ttcgccgtga ccgtaggaca
     7501 cgcgcgtttc ggggaaattc gctctcacat gtcagcaacc tgtccgacca cgctatcgcc
     7561 tcaacggcga ccggcgagca acgacaccga cgccggcccg gccggcatcg acccgaccgg
     7621 caccacgcgg ccgatcgcgt cgctgtcgca gcgacggggc gggaagcgtc gcgtcagtgc
     7681 aacgaacgac cggtcagtgc gatcaccgtc tcgccgaaga gctcgctcag cgacgggtgc
     7741 ggctggatca gcgccgccag ttcgtccacg gtcgcctccc agttgaccgc gagatacccc
     7801 tggccaagct gctcggtcac ccacggccca accatgtgca cccccaaaat ggtgccggcc
     7861 gttccgtccg cccgcttctc ggcgatgacc ttcaccaggc cgtcggtgtc gccgaggatc
     7921 tgggcgcgac cgttgccggc aaagcgatgc ttggatacca cgacctcgta ccccgcagcc
     7981 ttcgccgact cctcggagtg accggcgaac gcgacctccg ggtggcagta gatcgcccaa
     8041 ggcaccttgg agtagtccac cggtgccggg ttctcgccga ggatgtcctt gacgacgttg
     8101 atgccctcga tgaatccgat gtgtgccaac tgcggggtgt tgatgcaatc tcccaccgcc
     8161 cacacccccg gctcggcggt gcgacagttg gggtcgacga cgtagaagcc gcgttcatcg
     8221 atggcgacat cggttccctc gccaaccacg cccgacgaca ccggcgtgcg tccgatcgac
     8281 accaccacga tgtcgacgtc gaggccgtcg ccctcaccga aggtcaccgt cgtgccccca
     8341 gcggggcggg gctgatgacc ggtcaccgac acgccggttc ggatgtcgat gcccttcttc
     8401 ttgaacgcgc gctcgacgac cttggctgcg tctgagtcgc acccggaaag gatcttcggc
     8461 aacccttcga gaatcgtgac ggcgaccccc atgtcggcca gggtcgaggc gaactcgcaa
     8521 ccgatcgcac cgccgccgat gaccgccgcg ctcgacggca gtttgcgcag gtcgagaaac
     8581 tcgtcggagg tgaacacgat cgttccgtcc ggttcgaacc cgccgatcgt gcggggcacc
     8641 gatccggtcg ccaacacgat cgccgttccg agcagcgtcg actgcgagcc gtcgctgccg
     8701 accacgttga cctgatggga cggcccgagc agcccggtgc cttcgtagac atcgatcttg
     8761 cggcccttca caaggctctt gagcccgccg aacatggtgt cgacgacgcg ctgcttgcga
     8821 tcgatcgtca cgttccagtc gattccggtc tcttcggaga acacgccgaa ctcggccgaa
     8881 tgcttcacgg cccggcgcac cgccgcggtt tccaacagct ccttggccgg gatgcagccg
     8941 cggtgcaggc aggttcctcc gagcttgtcc ttttcgatga gtgcgatctt caggccggcg
     9001 ctcgcgccat acaacgcggt cgcgtaaccg ccaggtccgc cgccgatcac gacgacgtca
     9061 tagagcgagg ggttgatctg ttccacggga gtcctcttgg tcgaagagct tggtcgttga
     9121 gctcggtcgc cggcgcgaat ctgacgcgac caggtgcgac cctgcgagca taggtccccg
     9181 cgaccgccct cagcgcgaga ccatccagaa ctgaacggcg gctgcggcga ggatcaccac
     9241 accacacacc acggccatcg tgatcagcat tcgagtactc acgcgaaaca gcgtacggga
     9301 cgtgagcccc cgccccgatt ccgataccct gccaagcgga ttcacccgcc ccgcacccaa
     9361 cctctgggag acgcccccat gcgcgacgct gtcggcaaca tcgcttcgat tctggttctc
     9421 ggaggtggct ctgaaattgc ggtcgagacg atcaaggcgc tcaccaaacg gcgtcttcgg
     9481 cgagtcgttc tcgccgtgcg ctcccccgac aaggtggccg acgttgtcga cgatctgcga
     9541 tcgcgtggcc tcgaggtcgc cacgatcccg ttcgacgggg ccgacatcgc ctcccacgac
     9601 cgggtcatcg cctcggcctt cgacgacggt gacttcgacg tcgtgttgtc cgccttcggt
     9661 cagctcggcg atcaggacga actcgacgac gacccgaccg ccgccggcga actcgcggtc
     9721 gtgaactatg cgggccaggt gtcggcgctc accgccgtcg cgaaacagct gaaaatccaa
     9781 ggccatggaa cgatcgtcgt gttgagctcg gtcgccggcg aacgggtccg caaagacaac
     9841 gccacctacg gttcgaccaa ggccggactc gacggattcg cccaaggcct cggcgaccgc
     9901 ctccgtggaa ccggcgtcgg cgtgatgatc gtgcgaccgg gattcgtcca taccaggatg
     9961 accgagggca tggaccctgc tccgctgtcg acgaccccgg acgcggtggc cgccgacatc
    10021 gtgtcgggat tggaccgagg cgcgacggtc atctggtcgc cactggactc gccgatgcca
    10081 ccgcggcgct gggcaacata cccgacgtgc tgagcggcct tccctggtcg ccgaaggtcg
    10141 atcgactgat ctcggagggg cgtccagcgc gcatcgacga cggcaacggc gaggacttcg
    10201 gaggcctggt gctcccggag ccaaatagtg cactgctgtg tccgctgctg atgaacggac
    10261 gcgtctacgg tctcgttg
//
LOCUS       DAOOMA010000023        10117 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_7614_length_10117_cov_4.891274, whole genome shotgun sequence.
ACCESSION   DAOOMA010000023 DAOOMA010000000
VERSION     DAOOMA010000023.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 10117)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10117)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10117
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7614_length_10117_cov_4.891274"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..761
                     /locus_tag="PLP95_01400"
     CDS             <1..761
                     /locus_tag="PLP95_01400"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HPB44487.1"
                     /translation="ETTPETTPDTTPVTIWQPPPVPTVLGPTGAPMKPPVVRPAAAWL
                     PNGVPAPGSMFRDLAADESLAPMGPVVALTFDDGPSQYTTQIVNLLKLYGVPATFFQL
                     TPQSQQRADLVRQMQADGFRLGVHTKNHVKLADADPGTQIDQIVGSIDQLEAITGPGT
                     VRCFRPPYGSYDQYVLDLVASKGLATAMWSIDTLDWEKPDWFTIVGRVLNGAQDRSVV
                     LMHDGGGDRTATILALPWIIQGLRDRGFTFVAVC"
     gene            836..908
                     /locus_tag="PLP95_01405"
     tRNA            836..908
                     /locus_tag="PLP95_01405"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:869..871,aa:Thr,seq:cgt)
     gene            1021..2094
                     /locus_tag="PLP95_01410"
     CDS             1021..2094
                     /locus_tag="PLP95_01410"
                     /inference="COORDINATES: protein motif:HMM:NF022427.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2804 family protein"
                     /protein_id="HPB44488.1"
                     /translation="MNADAVATVNPSLPPDPVAPPPAVVIDGSYRFGTYGAAIPVVNL
                     LDASGSSPVRRALRGLRTKEWQAFQAGDDDTFILGAVYTAKVSGLLQVLVVNKAAATI
                     QRWQKLVPGWVPAVAHGLSGTRSHGHAGGFSVTFTNEFDDGVVRIDATHPGRGDLAPL
                     ELHGVGRCGADDAGHLVIVHPFADGRALYSHKTMMPFDASVRVGSDEIGLAEDRGFLI
                     IDDHHGEYPRPQRYDWLTAVRRSPTGLIEGFNLTANQVRDPEVFNENALWIANAVHRL
                     PAITVERPNGPWGKWHARDRSGAVDVTFTPTVRNSLHLGPRRCLAEYYAPYGWFEGAI
                     DTGSARLELDGFFGMGEQKLIRL"
     gene            2206..2505
                     /locus_tag="PLP95_01415"
     CDS             2206..2505
                     /locus_tag="PLP95_01415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB44489.1"
                     /translation="MARTAPIVAASLVGGYLVARETKKRELGGIVLAVGGVTAFRSWV
                     KSVGTAKALLLLAVYLVGFGGSHPLAKKIGAWPSVLTVSAAAATAAHIVADRSAD"
     gene            complement(2561..5641)
                     /locus_tag="PLP95_01420"
     CDS             complement(2561..5641)
                     /locus_tag="PLP95_01420"
                     /inference="COORDINATES: protein motif:HMM:NF024867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC family ATPase"
                     /protein_id="HPB44490.1"
                     /translation="MRPHRLQVTAFGAFAEHQDIDLDRLAEEGLFLIHGRTGAGKTTL
                     LDAIVFALYGEVGHRGSDRLVSHHVEIGTTPRVELEFTIAGHRYRVTRSPSYVAPKKG
                     GGTTQKAASATLVRLEPDGTETVLGSKQTDVNHEIQSLVGLNHLQFSQVMLLPQGRFE
                     QVLRAKSDERQKLLDTLFDTSLYASATQWLEARAGEQRKILEGAGRDLEAVMQQALDL
                     RTALLQREDQLDLSEPDDSGPDITWLTNVVHEVDQAATLAAADAESDRATEATAASML
                     TDAKLTLSQWDQADRARSHLRQLAAAQPEIDQLEVRLAEFDAANAIAVPLRDEHNAKR
                     RLDTAWQRLTEAVATIRTALDEATVLDEGFATLSIDTAVDIANFEAGRVEPFHQQVSS
                     IVAIARAGLEQRARLSAELEQANAARTHTAQELADAEQRERSTIDTISDLEQRHRELS
                     AQLDTESEVAAQLELRQADLSRLEAQLGAAKSIARLEQQRQTAESTRQAAVDAHQAAV
                     DDAQALRGRYFDGIAAVLAASLTDGEPCSVCGSLDHPSVAQSSDDAVRPDDLEAADGV
                     VETLAAEREQATTIVSQLTTKIAEQRGILGEVSEASSSVEVLSDRRADLEASVQAAER
                     AGGTATRVQRELQEVDGSLIAARAERAEATALRAELAGRQATEGDQIAQAQAALDQLG
                     DMGGLDATRAQQLLIGVDTASHALRSAATQHRDAASELDRARIDAENAVRGSTFADRA
                     TPTAILSAYQREGGDRDVWFARINDHHHGLDSATELLAHDWVTALPQQRPSVADAESA
                     ARFATDASRRASTRETHLKAARDDLRKLSERFDTQLAQYEVQRSTVELLSHVADRVAG
                     KRKPRVSLQRWVLRSYLEEICEYANQRLVTMTSGRFRLEVRETPTSGNSQAGLDLDVF
                     DAHTGQTRNVTTLSGGETFQASLALALGVADTVSAHHGGIRLDALFVDEGFGSLDPES
                     LQLAMDELDRLREGGRMVGLISHVGALRERIGFGVEVIATPKGSLARLGEITPV"
     gene            complement(5638..6936)
                     /locus_tag="PLP95_01425"
     CDS             complement(5638..6936)
                     /locus_tag="PLP95_01425"
                     /inference="COORDINATES: protein motif:HMM:TIGR00619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HPB44491.1"
                     /translation="MRFLHTSDWHLGRTFHGGSLLDEQRAMVDAIVEIAEQHHVDAVI
                     IAGDLFDRAIPPVEAVELWESAVARLRLTGAVVIAISGNHDSARRVSVHSRLLESGGV
                     FLRGSIECCDEPILVESRHGAAPVAVYAVPYLDPVAARSVFGEAAFEPLEAGSIVADD
                     ASASDGDGDGDGDGDGADIDAETDAETDRPIRRRRVTHRSITAAAMASVRADLETREV
                     SGTVVVAHTFVGGGVSSESERDITLGNVEQVPLTVFEGIGYVALGHLHGPQVFEGGRI
                     TYSGSPLPYSFSEEHHRKCVRIIDLDADGAVDVETVQLPAIMRLATLSGTLDDLLHDS
                     SFADHEGSYVRAQLTDVHLPLNAMARLRDRFARAVELRHTPTGSELTGSATINAAQRR
                     ELPPLELTRSFFNDQEGRDPTDDELEWLDAALAQAMGVQR"
     gene            complement(7018..8910)
                     /gene="recD"
                     /locus_tag="PLP95_01430"
     CDS             complement(7018..8910)
                     /gene="recD"
                     /locus_tag="PLP95_01430"
                     /EC_number="3.1.11.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR01447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009338 - exodeoxyribonuclease V complex
                     [Evidence IEA];
                     GO_function: GO:0008854 - exodeoxyribonuclease V activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit alpha"
                     /protein_id="HPB44492.1"
                     /translation="MTRTSRTVALPSGVGSLAPFVDAGVFLDSEVQLAAVVQRLAVRC
                     GHEASDLLILGVAVAARGPRFGHVRVDLDTIADLHLDDHDEPAEVPWPDPQDWRDALN
                     AAPWAVATPHDAHLAPLRPLVFDGRNLYLQRYFIQEQRVGSDLLRRAGTEGLLGWSEQ
                     AIDTAIDRFFKPSDQDADAETPDWQRSAARGALSSPFSVLAGGPGTGKTRTIARMLAT
                     AQSLALDAGRPLLVGLAAPSGKAAARMNEAIASAIAELTSDELISDELAEVLRSAQAT
                     TIHRMLGANPRKGFARDRFHPVPFDIVVVDETSMVSLPLMAQLLDAVSDRTHLVLVGD
                     PDQLASVEAGTVMGDVVGPCVDDPTTGGSGTVAANGPLQRNVTVLRVARRFGTDSGIA
                     KLAAAVRRGDPTEVLATLRDDRNDVGWIEHADSPSLTALIDEVTDTARQAVLAAQDGE
                     VEVALEALSSLKVLAATHRQQLGVADWRGRIEERLIAEGLDVRSNERWYVGRPIIVTR
                     NDRLNGLANGDVGITIATDDSLAVAFEGNSGERRIIPTARLDHIDTWWSMTIHKSQGS
                     EFGHAIVSLPRPSVPILTRELLYTGITRARDRVTIIGSEESVVAATQRRVARASGLRE
                     RLWPEP"
     gene            complement(8907..>10117)
                     /locus_tag="PLP95_01435"
     CDS             complement(8907..>10117)
                     /locus_tag="PLP95_01435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB44493.1"
                     /translation="LIERTAGLVAFECHGDPGVARPRADTAQSEADRDKLTAAVLTRE
                     VPRVTGRWSFSAITALDHNRLNPDDPSLGDHGSDDEDQGESAGADVPTPAGDAAGRAV
                     GPTEVATASTTVTMPFGRLPAGPRFGTLVHSLFERCDFAADDPAVEVAGVLTDELAWQ
                     PIDLTPRSLVSATHADGIELLVDGMVRAFDTPLGDLFGGRRLGDFRRADRLDELSFEL
                     LVRPASTPLSDRDIGALIASHLADDHPLAPWAAGLAEGRFGVDLAGHLTGSIDAIFRI
                     ESAEGSRFVVSDYKTNRLGAFDAEPDPFDYLGTNLDRAMTQHHYPLQALLYSVALHRY
                     LRSRLAGYDPDLHLGGIAYLFVRAMIGPNTPVVSGATAGIWTWRPSSALITELSDLLH
                     DDVASAGGTR"
BASE COUNT         1577 a         3163 c         3600 g         1777 t
ORIGIN      
        1 cggagacgac gccggagacg accccggaca cgacgccggt gacgatttgg cagccacccc
       61 cggtgccgac ggtgttgggg ccgaccggag ccccgatgaa gcccccggtc gttcgcccgg
      121 ccgcggcgtg gctgcccaac ggtgtgccgg ctccggggtc gatgtttcgc gacctggctg
      181 cggacgagtc gctggccccg atggggccgg tggtggcgct caccttcgac gatggtccaa
      241 gccagtacac gacacagatt gtgaacctgt tgaagctcta cggagttccg gcgacctttt
      301 ttcaactcac gccgcagtcg cagcagcggg ccgatctcgt tcgtcagatg caggccgatg
      361 ggtttcgcct cggggttcac acgaagaacc atgtgaagct cgccgacgcc gaccccggca
      421 cgcagatcga ccagatcgtc gggtcgatcg accagctgga agcgatcacc gggccgggaa
      481 cggtgcgttg cttccgtccg ccgtacggca gctacgacca gtacgtcctc gacctcgtcg
      541 cctcaaaggg attagcgacc gcaatgtggt cgatcgacac cctcgattgg gaaaagccgg
      601 actggttcac gatcgtcggt cgagtgctga acggggcgca ggatcgcagc gtggtcctga
      661 tgcacgacgg cggcggcgat cgaaccgcca cgattctggc gctgccgtgg atcatccagg
      721 ggctgcgcga tcggggattc acgttcgtcg cggtgtgctg atcacccggg atcgcgtctc
      781 gcccggaacg gctttgccgc gtccgcggtg ggtgctggta tcttggtcgg ccgacgccca
      841 agtagctcag tcggtagagc aattcactcg taatgaatag gtcaggagtt cgattctcct
      901 cttgggctca cggaaacccc cgtaaacacg ggggtttcgt ccgttttcga ggcctgtcgg
      961 cgatgtcgga ggggtcggcc gacggggcgg tcccgcgagg tggttcgata gggtggctcg
     1021 atgaatgccg acgccgtcgc gaccgtgaac ccgtcgttgc cacccgatcc ggtggcacct
     1081 ccgccagcgg tggtgatcga cgggagctat cggttcggta cgtacggcgc ggcgattccc
     1141 gtggtgaacc tgttggacgc gtcgggctcg agtccggtgc gtcgggcgct gcgtggcctg
     1201 cggaccaagg agtggcaggc gtttcaggcg ggcgacgacg acacgttcat tcttggtgcc
     1261 gtgtacaccg cgaaggtgtc cggcctgttg caggtgctgg tggtgaacaa agccgccgcg
     1321 acgattcagc gctggcagaa gctggtgccg ggctgggtcc cggcggtcgc ccatgggctt
     1381 tccggcacgc gctctcacgg tcatgccggt ggattctccg tcacgttcac gaacgagttc
     1441 gacgacggcg tcgtgcgcat cgacgcaacc catcccggtc gaggcgacct tgcgccgctc
     1501 gaactccacg gtgttggacg gtgcggtgcg gacgacgccg ggcacctggt catcgtccac
     1561 cccttcgccg atggccgcgc gttgtactcg cacaagacga tgatgccctt cgacgcgtcg
     1621 gttcgggtcg gttctgacga aatcggactg gccgaagacc gcggcttcct gatcatcgac
     1681 gatcatcacg gcgagtaccc ccgaccccag cgctacgact ggctgaccgc ggttcgaagg
     1741 agtccgacgg gactcatcga ggggttcaac ctcaccgcca atcaggttcg ggatccggag
     1801 gtgttcaacg agaacgcgct gtggatcgcc aacgcggtcc accgccttcc cgcgatcacc
     1861 gtcgaacggc cgaacggacc gtggggaaaa tggcacgccc gcgatcgttc gggcgcagtg
     1921 gacgtgacgt tcaccccgac cgtgcggaac tcgctgcacc tcggccctcg acggtgcctc
     1981 gccgagtact acgcgccgta cggatggttc gaaggagcga tcgacaccgg ttcggcccgg
     2041 ttggaactcg acgggttctt tggcatgggt gagcagaaac tcatccgcct gtaggtccgt
     2101 ggggcctgta ggtctgtgtg gcggctaggc gccgcacggc ccgctccgtg gagtccgcaa
     2161 cggacgtgac ccggcagggt cgcggggcgt gggtaggctc acgacatggc tcgcactgca
     2221 cccatcgtcg ccgcctcact ggtcggcgga taccttgtcg cccgggaaac gaagaagcgt
     2281 gaactcggcg ggatcgtcct cgcggtgggt ggggtgacgg cgtttcgcag ttgggtgaag
     2341 tcggtgggca ccgccaaggc gttgctgttg ctcgcggtat atctcgttgg cttcggcggt
     2401 tcgcatccgc tggcgaagaa gatcggcgcg tggccgtcgg tgttgaccgt gtcggccgcg
     2461 gcggcgacgg ccgcccacat cgtggcggac cgttcagcgg actgacgcac ccggcgtgtg
     2521 tcgcctcgcc gacgctgcca ggccgagcgg tcgagcgcgc tcagaccggg gtgatttctc
     2581 ccaggcgggc cagcgacccc ttcggcgtcg cgatgacctc gacgccgaaa ccgatccgct
     2641 cgcgaagcgc tccgacgtgg ctgatcaaac cgaccatgcg cccaccctcg cggaggcggt
     2701 cgagttcgtc catcgccagt tgcaacgact cggggtcgag cgacccgaac ccttcgtcga
     2761 cgaacagcgc gtcgagtcga attccaccgt ggtgggccga gaccgtgtcg gcgacaccga
     2821 gcgcgagtgc caacgacgct tgaaacgtct caccccccga caacgtggtg acattgcggg
     2881 tctggccggt gtgggcatca aacacgtcga ggtcgagtcc ggcttggctg ttgcccgagg
     2941 tcggagtctc gcgcacctca aggcgaaagc gccctgaggt catcgtcaca aggcgttggt
     3001 tggcgtactc gcagatctct tcgagatagc tgcgaagcac ccaacgctgt agcgagacac
     3061 ggggcttgcg cttgcctgcg acccgatcgg cgacgtggct caacaactcg accgtcgatc
     3121 gttgaacctc gtactgggcg agttgggtgt cgaatcgttc tgacagcttg cgcaggtcgt
     3181 cgcgtgcagc cttcaggtgc gtttctctgg tggaggcgcg ccgcgaggcg tcggtggcga
     3241 aacgcgctgc gctttcggcg tcggcgacgg aggggcgctg ctggggcagt gcggtcaccc
     3301 agtcatgggc gagcagttcg gtcgcggaat cgaggccatg gtggtggtcg ttgatccggg
     3361 caaaccaaac gtcgcggtcg ccgccctcgc gctgatatgc cgacaggatc gcggttgggg
     3421 tggcacggtc ggcgaaggtc gagccccgca cggcattttc cgcgtcgatg cgggcgcgat
     3481 cgagctcgct cgctgcgtcg cggtgttgcg tcgccgcact gcgaagtgcg tgcgatgcgg
     3541 tgtcgacgcc gatgagcaac tgctgtgccc gggttgcatc gaggcctccc atgtcgccga
     3601 gctgatccag ggccgcctgt gcctgggcga tctggtcgcc ttcggtcgct tgacgccccg
     3661 cgagttccgc tcgcagcgcc gtcgcctcgg cgcgctccgc tcgggcggct atcaggctgc
     3721 cgtccacctc ctggagttcg cgttggacgc gtgttgcggt gcctcccgcc cgctcggcgg
     3781 cctgaacgga ggcctcaagg tccgctcgcc ggtcggagag cacctcgacg gacgacgacg
     3841 cttcggatac ctcgccgagg atgccgcgtt gttcggcgat cttggtggtg agctgtgaca
     3901 cgatggtcgt ggcctgctcg cgttcggcgg cgagtgtttc cacgaccccg tcggccgctt
     3961 cgagatcgtc gggccggacc gcatcgtcgc tcgattgcgc gaccgacggg tgatcgagcg
     4021 aaccgcatac cgaacagggc tctccgtctg tgagcgacgc ggcgagcacc gccgcgattc
     4081 cgtcgaaata gcgcccgcgc aatgcctgag cgtcgtcgac cgcggcctga tgggcgtcga
     4141 ccgccgcctg acgagtggac tcggcggtct ggcgctgctg ctcgagtcgg gcgatcgact
     4201 tggcggcacc aagttgcgct tcgaggcgac tcaggtcggc ctgtcgtagc tcgagttggg
     4261 cggcgacctc gctttcggtg tcgagctgtg cgctcagttc ccggtgacgc tgctcaaggt
     4321 cggagatcgt gtcgatagtc gagcgttcgc gctgttcggc atccgcgagt tcctgagccg
     4381 tgtgcgttcg tgcagcgttc gcctgttcga gttcggcact caaccgagcc cgctgttcga
     4441 gcccagcgcg agcgattgcg acgatcgagg agacctgctg atgaaacggc tcgactcgac
     4501 cggcctcgaa gttcgcgatg tcgacggcgg tgtcgatcga gagcgtcgcg aaaccctcgt
     4561 caaggacggt tgcctcatcg agtgccgtgc ggatggtggc gaccgcttcg gtgagtcgtt
     4621 gccaggcagt gtcgaggcgc cgcttcgcgt tgtgttcgtc gcgaagcgga accgcgatgg
     4681 cgtttgcggc gtcgaactcg gcgagtcgaa cctcgagttg atcgatctcg ggctgcgctg
     4741 cggcgagctg gcgaaggtgg ctgcgtgccc ggtcggcctg atcccactgg ctgagcgtca
     4801 gcttggcgtc ggtcagcatg ctggcggccg tggcctcggt ggccctgtcg gactcggcgt
     4861 cggcggccgc gagtgtcgcg gcctggtcga cctcgtgaac gacgttggtg agccaggtga
     4921 tgtcgggccc cgagtcgtcg ggctcgctga gatctaactg gtcctcgcgc tgaagcagcg
     4981 cggttcgaag gtcgagggcc tgctgcatta cggcttcgag gtctcggccc gcgccctcga
     5041 ggatcttgcg ttgctctcct gctcgcgctt cgagccactg cgtggcactc gcgtacagcg
     5101 aggtgtcgaa gagggtgtcg agcaacttct gacgttcgtc ggacttggct cgcaacacct
     5161 gctcgaagcg gccctggggc aacaacatga cctgactgaa ctgaagatga ttgagtccga
     5221 ccaacgactg gatctcatgg ttgacgtcgg tctgctttga gccgagcacg gtctcggttc
     5281 cgtcgggttc gaggcggacc aacgtcgccg acgccgcctt ctgggtggtt cctccgccct
     5341 tcttgggtgc gacgtaggag ggcgatcgcg tcacccggta acggtgacct gcgatggtga
     5401 actccagttc aaccctcggc gtcgtgccga tctcgacgtg atgggagacc aggcgatcgg
     5461 agccccgatg cccgacctcg ccgtagagcg cgaacacgat cgcatcgagc agcgtggtct
     5521 tgcccgcgcc ggtgcgaccg tgaatgagaa acagcccttc ctcggcgagt cggtcgaggt
     5581 cgatatcttg atgctcggcg aaggcgccga acgctgtgac ctgaagtcgg tgcggtctca
     5641 tcgttgcact cccatcgcct gtgcgagcgc ggcgtccaac cactcgagtt cgtcgtcggt
     5701 ggggtcgcga ccctcctgat cgttgaagaa ggacctcgtg agttcaagtg ggggcagctc
     5761 gcgccgttgt gctgcgttga tggtcgccga accggtgagc tctgagccgg tgggggtgtg
     5821 gcgcaactcg acggcccggg caaaccggtc gcgaagtcgg gccatcgcgt tgagcggtag
     5881 gtgcacgtcg gtgagctgtg cccgaacgta ggacccctcg tggtctgcga acgaggagtc
     5941 gtggaggagg tcgtcgagtg tgccactcag tgtggcgaga cgcatgatcg cgggtaactg
     6001 gaccgtctcc acatcgacgg ctccgtcggc gtcgaggtcg atgattcgca cgcacttgcg
     6061 atggtgctcc tcggagaacg aatagggaag cggtgaaccg gagtaggtga tgcgcccacc
     6121 ctcgaaaacc tggggtccgt gaaggtggcc cagcgcgacg tatccgatgc cctcgaaaac
     6181 cgtgagcgga acctgctcga cgttgccgag ggtgatgtcg cgctcggact ccgaggagac
     6241 gccaccaccc acgaacgtgt gcgccacgac gaccgttccc gacacctcgc gcgtctcgag
     6301 gtcggcgcgc acgctcgcca tcgccgcggc agtgatgctg cggtgggtga cgcgtcgacg
     6361 ccggatgggt cgatcggtct cggcgtcggt ctcggcgtcg atgtcggcgc cgtcgccgtc
     6421 gccgtcgccg tcgccgtcgc cgtcgctggc gctggcatcg tctgcgacaa tcgaacccgc
     6481 ttcgagtggc tcaaaggcgg cctcgccgaa tacggagcga gcggccacag ggtcgaggta
     6541 cggaaccgcg tacaccgcga cgggtgcggc cccgtgtcgc gattccacca ggatcggttc
     6601 gtcgcagcat tcgatggatc cgcgcaggaa cacgccgccg gattcaagca atctgctgtg
     6661 caccgacacg cgacgggcgg agtcgtgatt gccggagatg gcgatgacga cagcgcccgt
     6721 cagacgcagc cgcgcgacag cggactccca cagctcgacc gcttcgaccg ggggaatggc
     6781 gcgatcgaac agatcgccgg cgatgatgac ggcatcgaca tggtgctgtt cggcgatctc
     6841 gacgatcgcg tcgaccatgg cgcgttgttc gtcgagcagt gagccgccgt ggaaggttcg
     6901 gccgagatgc cagtcggagg tgtgcaggaa ccgcatgggt cacaacctag aagcgaccgc
     6961 tgacactcag aatggttatg tcctcgatcg tggcggcgat gacggtggcg gtgtgcgtca
     7021 cggctccggc cacagtcgct cccgcagtcc ggaggcgcgc gccacccgtc gctgggtggc
     7081 ggcgaccacc gactcctccg aaccgatgat ggtgacccgg tctcgcgccc gggtgattcc
     7141 ggtgtagagc agttcacggg tcaggatcgg aaccgacggg cgcggaagcg acacgatcgc
     7201 atggccgaac tcggatccct ggctcttgtg gatggtcatc gaccaccagg tgtcgatgtg
     7261 gtcgaggcga gcggtcggaa tgatgcggcg ttcaccggag ttgccctcga aggcgaccgc
     7321 aaggctgtcg tcggttgcga tggtgatgcc gacgtcgccg ttggcgaggc cgttgagacg
     7381 atcgttccgg gtgacgatga tcggtcggcc gacataccaa cgctcgttgg atcgcacgtc
     7441 gagtccctcg gcgatcagac gctcctcgat gcggccccgc cagtcggcga cgccgagttg
     7501 ttgccggtgc gtggcggcga gcaccttgag cgacgacagg gcttcgagtg cgacctccac
     7561 ctcgccgtct tgggcggcga gcaccgcctg gcgggcggtg tcggtcacct cgtcgatcag
     7621 cgccgtgagc gacgggctgt cggcgtgttc gatccatccc acatcgtttc ggtcgtctcg
     7681 aagcgtcgcg aggacctctg tagggtcgcc ccggcgaacc gcggccgcaa gcttggcgat
     7741 gccggaatcg gttccgaagc gtcgggcaac ccgcaacacg gtcacgttgc gttgcagcgg
     7801 accgttcgcg gcgacggtgc ccgagccccc ggttgtcggg tcgtccacgc aggggcccac
     7861 gacgtcgccc atcaccgttc ccgcttcgac cgaggcgagc tgatcgggat cgccgacgag
     7921 gacgagatgg gtgcgatcac tcacggcgtc gagcagctgg gccatcagcg gcagcgacac
     7981 catggacgtc tcatccacga cgacgatgtc gaagggcacc gggtggaatc gatcccgagc
     8041 gaaccccttg cgcgggttcg cgccgagcat ccgatggatc gtggtcgcct gagcggagcg
     8101 caacacctcg gccaactcgt cgctgatgag ttcgtcggag gtcaattctg cgatagcgga
     8161 cgcaatcgcc tcgttcatcc gtgccgccgc cttgccggat ggcgccgcga gaccgaccag
     8221 gagcggccgg ccggcatcga gcgccagcga ctgcgccgtg gccaacatgc gtgcgatcgt
     8281 gcgggtcttt ccggtgcccg gcccgccggc gagaacgctg aaggggctgc tgagggcacc
     8341 gcgcgcggcg ctgcgctgcc aatcgggagt ctcggcgtcg gcgtcctggt cggatggctt
     8401 gaagaagcgg tcgatcgccg tgtcgatcgc ctgctcgctc cacccgagta gtccttccgt
     8461 gccggcccgg cggagaaggt ccgatccgac ccgttgctcc tgaatgaagt aacgctgaag
     8521 gtagagattg cgtccgtcga acacgagggg acgaagtggg gcgaggtgcg cgtcgtgcgg
     8581 agtggcgacc gcccatgggg cggcgttcaa cgcgtcgcgc cagtcctgcg gatccggcca
     8641 tggaacttcg gccggctcgt cgtggtcgtc gaggtgcagg tcggcgatcg tgtcgagatc
     8701 gacccgcacg tggccgaatc ggggcccgcg cgccgccacg gcgacgccga gtatgaggag
     8761 gtcggacgcc tcgtgaccgc agcgaacagc gaggcgttga acgaccgcag cgagttgcac
     8821 ctcgctgtcg aggaacacgc cggcgtcgac gaagggtgcg agcgagccaa ctccgctggg
     8881 cagtgccacg gttcttgagg tgcgagtcat cgggtgcctc cggcgctggc aacgtcgtcg
     8941 tggagcaggt cgctcagctc ggtgatcagt gcgctggatg gccgccaggt ccagatgccc
     9001 gcggtggctc cggagacaac gggtgtgttc ggcccgatca tggcgcgtac gaacaggtac
     9061 gcgatgccac cgagatggag atcggggtcg taccccgcga gtcgggagcg caggtaacgg
     9121 tgaagcgcca ccgaatacag caacgcctgg agcgggtagt gatgttgggt catcgcccga
     9181 tcgaggttgg tgccgaggta gtcgaacggg tcgggttcgg catcgaaggc gccaagacgg
     9241 ttcgtcttgt agtcggacac gacaaagcgc gagccctcgg cggactcgat gcggaagatg
     9301 gcgtcgatcg agccggtgag gtggccggcg aggtcgacgc cgaatcgacc ctcggccaat
     9361 cctgccgccc acggggcgag tggatgatcg tcggcaaggt ggctggcgat gagagcgccg
     9421 atgtcgcggt cgctgagggg tgtggatgcg ggccggacaa gcaactcgaa ggacagctcg
     9481 tcgaggcggt cggcacggcg gaagtcaccg agtcggcgac cgccgaacag gtctcccaac
     9541 ggcgtgtcga aggcccggac catcccgtcg acgagcagct cgatgccgtc ggcgtgtgtg
     9601 gccgacacga gcgatcgtgg tgtgaggtcg atcggctgcc aggcgagttc gtcggtgagc
     9661 acaccggcga cctcgacggc tggatcatcc gcggcgaagt cgcatcgttc gaagagcgaa
     9721 tggaccagcg tgccgaaccg ggggccggcg gggagccgtc cgaagggcat cgtcacggtc
     9781 gtcgaagcag tggcgacctc ggtcgggccg acggcgcgac ccgcagcgtc acccgccggg
     9841 gtcgggacat cggcgccggc cgactcgcct tggtcctcgt cgtcggagcc gtgatcgccc
     9901 agcgagggat cgtcggggtt caaccggttg tggtcgagcg ccgtgatggc gctgaacgac
     9961 caccgtccgg tcacccgcgg tacctcgcga gtgagcacgg ccgccgtcag cttgtcgcgg
    10021 tctgcctcgc tttgagcggt gtcggcacgt ggccgtgcga cacccggatc gccgtgacac
    10081 tcgaaggcga cgagtcccgc ggtgcgctcg atgaggg
//
LOCUS       DAOOMA010000024         9826 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_7974_length_9826_cov_4.081466, whole genome shotgun sequence.
ACCESSION   DAOOMA010000024 DAOOMA010000000
VERSION     DAOOMA010000024.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 9826)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9826)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9826
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7974_length_9826_cov_4.081466"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            182..835
                     /locus_tag="PLP95_01440"
     CDS             182..835
                     /locus_tag="PLP95_01440"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HPB44494.1"
                     /translation="MAKTSVAKTPPPRAKRMNAADRRSSILNAARKAFSESGDMAGTT
                     MRTIADRAGISEGIIYRHFENKDQLFFEAVVEPLRDAVDSLVAATEGIDSETPLTGER
                     QIEALAGLYSQLIATLTEVLPLLGLVLFGDPKVARRFYREHFEVAMDRLAGAWAEVEQ
                     RYGLEVDLTAISARAVMGTALILAIESKYAKEHPSRADLSRSAALGTVEGFFPTLNG"
     gene            904..1656
                     /locus_tag="PLP95_01445"
     CDS             904..1656
                     /locus_tag="PLP95_01445"
                     /inference="COORDINATES: protein motif:HMM:TIGR00044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HPB44495.1"
                     /translation="MADMTRPPETVEEFRRNLDEVRSRIVDSACRVDRDPAEVRLLPV
                     SKTVPESRLINAITAGCTQLGENKVQEAKRKAANLAHLGVSWSVIGHLQTNKAKNVAA
                     FADEFQALDSLRVAEALDRRLQALGRSLDVFVQVNTSAEESKFGLPPEEVDGFVAELR
                     HFHALRPVALMTLAVFSDDHTRVRDCFIRLRTIRDRLRETRPDGMGLEELSMGMSGDF
                     ELAIEEGATVVRVGQAIFGPRGLPDSHYWPTT"
     gene            complement(1752..2267)
                     /locus_tag="PLP95_01450"
     CDS             complement(1752..2267)
                     /locus_tag="PLP95_01450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB44496.1"
                     /translation="MNTTSTTPNRARRRLAAMAAVGAALAAGCAPQTPSGPKVVGQPC
                     DAGEGVTVVVDFQKLNNTVAIGCAPGTQASGLAALTNAGFTYDEIGGFACQINSLPVE
                     GYPACQFDAYWSYNKSDGTSPWDYSMVGASSGPIAVDSLEGWSYSVITPDNYDGAPIR
                     LSVEKVLARRG"
     regulatory      complement(2355..2489)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            complement(2567..3538)
                     /locus_tag="PLP95_01455"
     CDS             complement(2567..3538)
                     /locus_tag="PLP95_01455"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024989.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003960 - NADPH:quinone reductase activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH:quinone oxidoreductase family protein"
                     /protein_id="HPB44497.1"
                     /translation="MRRVVCTEWGAPEGLEVQEHPDLEPGPGQVVVEVAAAGVNFVDA
                     LFVAGTYQIKIPPPFTPGSECAGVISALGEGVEGLDVGDRVIAAGGLGGYASQIAVSA
                     AAVTRVPDDLSLAMAAAVVQSYCTAWFTLTRRISVVSGETVLVLGAGGGVGLAAIDVA
                     RSLGATVIAAASSAEKLEAALVAGASATIDYSTEPIKDRARELSPGGVDVVIDPIGGD
                     ATEASLRALRFGGRLAVIGFAAGTIPKIPTNQVLLNNRSVVGVDWGAWAMHNPADQGV
                     LLREVLDAVVAGTLSPAAPSERPLDEAAAVLREALDRRVVGKVVLVP"
     gene            3609..4394
                     /locus_tag="PLP95_01460"
     CDS             3609..4394
                     /locus_tag="PLP95_01460"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HPB44498.1"
                     /translation="MSRLAVVTGSAGGIGAATATTLEAEGYEVIGVDIAGAEITADLS
                     TPGGRSAMVEAVTERTGGTLDVLVANAGVMDTAEMCLRVNYFGAVATLEGLAPLLAHG
                     ESPRAVVTVSSSILNVQMPAVVEACLAGDEAQAIELASAPGAMDMAGYPTSKYALARW
                     LRRSAIGDAWAGAGIALNGVAPGIVQTNMTAEMLAHEDSKELADASVPMPFGGYCHPQ
                     HVADVIAFLASARPTRICGQILFVDGGADVVLRGDDIWHTAAI"
     gene            4427..5518
                     /locus_tag="PLP95_01465"
     CDS             4427..5518
                     /locus_tag="PLP95_01465"
                     /inference="COORDINATES: protein motif:HMM:NF014954.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HPB44499.1"
                     /translation="MGPIADRPDQLTPAWLTDALRTAGHLQVDSRVATVDMQPIGTGQ
                     MCDSMRVRVAFDGETDLPDTFVAKFPSTDASSVAAGKLMRAYEKEVRFYQHLAHSLPV
                     NSPAMYLADIDVDSGSFVLLLEDRSPAVQGDQIAGTTPEVARLAVAELVQLHAPRWGD
                     DSLESFDWMARATRDQRDLLSSMLPTLWQEFLARYGGAVEAHLRRAGGFFVEHIANYL
                     DADVIGTGITHSDYRLDNLLFAPGVFAGTTEGRPTVVDWQTVAYGCAPYDVSYFLGSS
                     VEPPIRREIEWELLAEYHRGLVANGVQDYSQASLEADYRRGTWAGMLIGIVAAVMVER
                     TERGDTMFLTMLDRHARHALDLDADETIR"
     gene            complement(5579..6877)
                     /locus_tag="PLP95_01470"
     CDS             complement(5579..6877)
                     /locus_tag="PLP95_01470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012224393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HPB44500.1"
                     /translation="MNDSSSAGFGLSGDSLADELASIEFWTQPLESRAARLAEIREIA
                     PFVHTYQPNPLTLENEWYWLSTRYDDVIEISRRPKEFCSGQGSVSIADMPTDIMEFFG
                     SFIVMDDPRHARQRGIVSRNFGPRQLQGLLDSVQHVADEVIDRFCEQGEVDLVSAFSE
                     PFPLLIICDMMGIPRSEFDSVLRCTNVILGAGDPDTVGEGEDLFTALIGAGTELSVLL
                     GELARDRRANPKDDLTSALVNAEPGEDVLTPEELAPFFILLAVAGNDTTRNAITYGVD
                     FLTRNPDQRAIWQGDIDGVTPTAVEEIVRMGSPVTFMRRTATCEVTLSGHDFHEGDKV
                     VMLYGAANRDPREFADPERFDVRRSPNNHVGFGGPGPHFCLGAHLARRELSVAFRTFF
                     NRLGDMEATAPAVPLNALGVPLVSGIKSLPVRFTPSKPLG"
     gene            complement(6909..7994)
                     /locus_tag="PLP95_01475"
     CDS             complement(6909..7994)
                     /locus_tag="PLP95_01475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase domain-containing protein"
                     /protein_id="HPB44501.1"
                     /translation="MAKAPAGLVNAAGREIRVSSPDRIVFEATDRTPAITKLQVCEYF
                     AAVGETMMASLGERPTALERWPDGWRDGMRLALGPQDPDGAGFYQKRLPRGAPEFIET
                     VRVTFPSGRTADELCPTEPAAFVWAAQMGTLTFHPWPVRRRDVDHPDEFRIDLDPQPG
                     TGFSDAVRVAGVARELLEELGLRGFAKTSGNRGIHIYVRIQPRWSFEDVRHAAIGFGR
                     ELERRDGGVTTAWWKELRGSRIFVDFNQNNRDRTIAGAYSIRARAGAPVSMPIAWDRL
                     GDLEDPTQFNLLTVPDLLASEGDPWREMEDVEYSLDPLLQLWQTLPGGELNFPPDYPK
                     MPGEPPRVQPSRKVESHWDAQGRRIGP"
     gene            complement(8060..>9826)
                     /locus_tag="PLP95_01480"
     CDS             complement(8060..>9826)
                     /locus_tag="PLP95_01480"
                     /inference="COORDINATES: protein motif:HMM:NF019581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HPB44502.1"
                     /translation="APTVLIRNGRVFDGAGSPSVEADVLVRDGVVAAIGLGLVAPAAA
                     TVVDADGCWVTPGFIDLHTHYDAELEIAPSLGESVRHGVTTVLIGSCGLSFVMGEPED
                     LADQFCRVEGVPRDVVLPLLETTKNWDDPSGYLAHLRSLPLGPNVTLFLGHSAVRTSV
                     MGLERAVDASAKPTRSERREMLRLLDEALDAGYLGMSFNTLPWDKLGGDRFNSRPTPS
                     VFATFGEYWSFAKVLRERGRIFQVVPDLQGRWNIPVLMAMSAGVGRPALRMNLLSMLD
                     PVVAHGSHRFIGATTNAFNRVLRGDVRFQAVPLVFDLFVDGLDVPVMEEFGAGTQALA
                     VGDLEARRRLMSDPDFRARFRKQWNNQLAPRAYHRKLDEPVIVGCPDETLVGKTFGEV
                     AAKRGVDAIEAFMDLQAQFGHELRWRTVVANDRPRSVAQIANHPAAIMGFADSGAHLR
                     NMAFYDLPLHLLRLAASADGGGGDDGGGGDDEPVLSPERAVYRMTGEIADYLGIDAGR
                     LVVGGRADIAVVDPATLDERLDNIVEDDSADVVGVDRLVRRHDECVTHVLVNGRLAWS
                     DGAFVEGFGAQGGYGRVLTRVD"
BASE COUNT         1698 a         3489 c         3087 g         1552 t
ORIGIN      
        1 ccctggctga gttcggtgaa ccccggattc tccggtgatg cgagtaccct agtcgagtaa
       61 ggacttactc tcaacgcgat gagagtaacc gatcgctctc acaattcaac tcccgcacta
      121 tcatggctcg ggcgaggcac acgtgcgctg gggcctcacg cgtcgcgaaa gcaggatcct
      181 catggcgaag acgtcagtgg ccaagacacc gccaccgcgg gccaaacgaa tgaacgcagc
      241 cgatcggcga agttccatcc tcaacgccgc ccgcaaagcc ttcagcgagt ctggagacat
      301 ggccggcacc accatgcgca ccatcgcgga tcgtgccggg atcagcgaag gcatcatcta
      361 ccgccacttc gagaacaagg atcaactgtt cttcgaagcg gtggtcgaac ccctccgcga
      421 cgccgtcgac agcctcgttg ccgccaccga gggcatcgac tccgaaaccc cactgaccgg
      481 cgagcgccag atcgaagccc tcgccggcct ctattcacaa ctcatcgcca cgctcaccga
      541 ggtgctgccg ctgctcggac tcgtactttt cggcgacccg aaggtggcgc gacgcttcta
      601 ccgcgagcac ttcgaggtgg cgatggatcg cctcgccgga gcgtgggcgg aggtcgaaca
      661 gcgttacggc ctcgaagtcg atttgaccgc gatctccgcg cgcgccgtca tgggcaccgc
      721 gctcatcttg gccatcgagt cgaagtacgc gaaggagcac ccctcgcgag ccgacctgtc
      781 gcgcagcgcc gctctcggca ccgtcgaggg tttcttcccc acgctcaacg gctgaaccgc
      841 gccgcatgcg tcgtcgtcgc tctgacgtct tcgcgccctg atcggtccca ctactgtgcg
      901 cgcatggctg acatgacccg accacccgag accgtcgagg aattccggcg caacctcgac
      961 gaggttcgtt cgcggatcgt cgactccgca tgccgggtcg accgcgaccc cgcggaggtt
     1021 cgccttctac cggtctcgaa gaccgttccc gagtctcgat tgatcaacgc aatcaccgcc
     1081 ggctgcaccc aactcggcga gaacaaggtt caggaggcga aacgcaaagc cgccaacctc
     1141 gcccacctgg gggtgtcgtg gtcggtgatc ggtcaccttc agaccaacaa ggccaaaaat
     1201 gtcgccgcgt tcgcggatga gtttcaggcg ctcgattcgc tccgggtcgc cgaggccctc
     1261 gaccgtcgcc tccaggcgct cgggaggtcg ctcgacgtgt tcgtgcaggt caacacctca
     1321 gccgaggagt cgaagttcgg ccttccgccc gaggaggtcg acgggttcgt cgctgagctt
     1381 cgccacttcc acgcgctccg accggtcgcg ctgatgacgc tcgcggtgtt cagcgacgac
     1441 cacacccggg tccgcgactg tttcatcagg ctgcgaacca tccgcgaccg cctgcgcgag
     1501 acccgccccg acggcatggg cctcgaggaa ctctcgatgg gaatgagcgg cgatttcgag
     1561 ttggcgatcg aagagggggc gacggtcgtg cgggtcggcc aggcgatctt cgggccccgc
     1621 ggactccccg attcgcacta ctggcccacc acctgacccg ttcccaccac ctgacccgtt
     1681 ctccgataac cagtggtcgt gatcacgacc actggttatc ggagaacggg ccgcggcggg
     1741 tcggccgctg gtcagccgcg acgggccagc accttttcga cggagaggcg gatcggcgcg
     1801 ccgtcatagt tgtccggcgt gatcacgctg taggaccaac cctcgagcga atcgaccgcg
     1861 atcgggccgc tggaagcgcc gaccatcgag tagtcccacg gggaggtgcc gtcggacttg
     1921 ttgtagctcc agtaggcatc gaactgacat gccggataac cctcgaccgg aagcgagttg
     1981 atctggcacg cgaacccgcc gatctcgtcg taggtgaatc cggcgttggt gagcgcagcg
     2041 aggccactcg cctgagtgcc cggcgcacat ccgatggcaa cggtgttgtt cagcttctgg
     2101 aaatcgacca cgacggtcac tccctcgccg gcatcacacg gctgaccgac gaccttggga
     2161 ccgctcggag tctgcggcgc gcagccagcg gcgagcgccg caccgacggc ggccatggcg
     2221 gcgaggcggc gacgcgcccg attcggggtg gtggatgtgg tgttcatagt ttcctcctgg
     2281 ccgttgccgg gaggagcctg tcggtccgcc cgccacgacc ctcgttgtcc tcgacgatgg
     2341 ttgaagcacg tgccgatgtg gcacctgccg acgacgggcg atccggctcg catctcggtc
     2401 gagatgcaca cggctgcggg acagcgccgg agtctcaccg gcttcccccg aacgttcggc
     2461 tactggttat ctccgtggga atcacggcgc acgaagcgta ctccagacgc gtcacgtggc
     2521 acctcttgac caccgaccgg ccggccggtg gcgccgacag aggcgttcag ggaacgagca
     2581 ccaccttgcc gaccacccga cgatcgaggg cctcgcgcaa caccgcggcc gcctcgtcga
     2641 gtggccgctc gctcggcgcc gccggcgaaa gcgtccccgc tacgacggca tcgagcacct
     2701 cgcgcaacaa cacgccctga tcggccgggt tgtgcatcgc ccacgccccc cagtcgacac
     2761 cgaccaccga gcggttgttc aacagcacct gattggtggg gatcttcggg atcgttccgg
     2821 cagcgaatcc gatgaccgcc aaccgcccgc cgaatcgcag cgctcgcagc gacgcctcgg
     2881 tcgcatcgcc gccgatcggg tcgatgacga catccacccc tccgggcgac aactcgcggg
     2941 cgcggtcctt aatcggctcg gtcgaatagt cgatcgtcgc gctcgctccg gcgaccagcg
     3001 ccgcctcgag tttctcggcg ctcgacgccg ctgcgatcac cgtcgcaccg agcgaacgcg
     3061 ccacatcgat cgccgccagt ccgactcctc caccggcacc gagcacgagc accgtctccc
     3121 cggaaaccac gctgatgcgc cgggtcaagg tgaaccaggc cgtgcagtag ctctgaacga
     3181 ccgctgccgc catcgccagc gacagatcgt caggcacccg cgtcaccgcc gcggccgaca
     3241 ccgcgatctg cgacgcgtac cctccgagtc cccccgccgc gatgactcga tcgccgacgt
     3301 cgagcccctc caccccttcg ccgagcgcgc tgatcacccc ggcgcactcg cttcccggcg
     3361 tgaacggagg cggaatcttg atctgataag tcccggcgac gaagagcgcg tcgacgaagt
     3421 tgacccccgc ggccgcgacc tccacgacaa cctgtccggg acccggttcg aggtcggggt
     3481 gttcctggac ctcaagaccc tctggagccc cccactcggt acacacaact cggcgcatgg
     3541 gactcaccct atcgggggca tcgaacccgg gcgaccggta gcgtacggcc agaaccagga
     3601 ggattgtcat gtcacgtctc gcagtcgtca ccggctctgc cggaggcatc ggtgccgcca
     3661 ccgccaccac ccttgaggcc gaaggatacg aggtcatcgg tgtcgacatt gccggtgccg
     3721 aaatcaccgc agatctgtcg acccccggag gtcgctccgc catggttgag gccgtcaccg
     3781 aacgcaccgg cgggaccctc gacgtcctcg tcgccaacgc cggcgtcatg gacaccgccg
     3841 agatgtgcct acgcgtcaac tacttcggcg cggtcgccac cctcgagggc ctggccccgc
     3901 tgctggccca cggggagtca ccccgagcgg tcgtcaccgt gagctcgtcg atcctgaacg
     3961 tgcagatgcc cgccgttgtc gaggcctgcc tcgccggcga cgaggcccag gcgatcgagc
     4021 tcgcaagcgc gcccggcgcc atggacatgg ccggctaccc gacctccaag tacgcgctgg
     4081 cgcgctggct gcgtcgcagc gcgatcggcg acgcgtgggc gggcgcagga atcgcgctca
     4141 acggggtggc tcccggcatc gtgcagacga acatgaccgc ggagatgttg gctcatgagg
     4201 actccaagga gctcgccgat gcgtcggttc cgatgccctt tggcgggtac tgccacccac
     4261 aacacgtcgc cgacgtcatc gcctttctcg cctccgcccg ccccacgcgt atctgcggtc
     4321 agatcctgtt cgtcgacgga ggtgccgacg tggtgttgcg cggcgacgac atctggcaca
     4381 ccgccgcgat ctgagcactg cggacgagtc gaagccgaag caccccatgg gacccatcgc
     4441 agatcgcccc gaccaactca caccggcgtg gttgaccgac gccctgcgca cggccggcca
     4501 tctgcaggtc gactcccggg tggcgaccgt cgacatgcaa ccgatcggca ccggccagat
     4561 gtgcgactcg atgcgggtac gggtcgcctt tgacggcgaa accgacctgc ccgacacctt
     4621 cgtcgccaag ttcccctcga ccgacgcctc cagcgtcgcc gccggaaagc tcatgcgggc
     4681 ctacgaaaag gaggttcgct tctatcagca cctcgcccac agcctcccgg tgaattcccc
     4741 ggccatgtat ctcgccgata tcgacgtcga ctcggggtcg ttcgtgttgt tgctcgagga
     4801 ccggtcgcct gcggtccaag gcgaccagat cgcgggcacc acccccgagg tcgcccgcct
     4861 ggccgttgcc gagttggtgc aactccacgc cccgcgttgg ggcgacgact cgttggagtc
     4921 cttcgactgg atggcccgcg ccacccgcga ccagcgagac ctgttgtcgt cgatgctgcc
     4981 gacgttgtgg caggagttcc tcgcccgcta cggcggagcg gtcgaagcgc accttcgtcg
     5041 ggccggcggg ttcttcgtcg aacacatcgc caactatctc gacgccgacg tcatcggcac
     5101 cggcatcacc cactccgact accgcctcga caacctcctg ttcgcccccg gcgtcttcgc
     5161 gggcaccacc gaggggcgcc cgacggtggt cgactggcaa acggtcgcct acggctgcgc
     5221 gccctacgac gtctcgtact tcctcggctc gagcgtcgag ccgccgatcc ggcgtgaaat
     5281 cgagtgggaa ctactcgccg agtaccaccg cggcctcgtc gccaacgggg tgcaggacta
     5341 ttcgcaggcg tcgcttgaag cggactaccg acgtggcacg tgggcgggca tgttgatcgg
     5401 catcgtcgcc gcggtcatgg tcgaacgcac cgaacgcggc gacaccatgt ttctcacgat
     5461 gctcgatcgc cacgcccgcc acgcgctcga cctcgacgcg gacgaaacca tccgctgagc
     5521 caccaggtct gagtatccga gtccgagccg ccggcggaga gccgccggcg acgactcctc
     5581 agccgagcgg tttggacggg gtgaaccgca ccggcagcga cttgatgcca ctcaccaggg
     5641 gaacgcccaa cgcattgagc ggcaccgccg gcgccgtcgc ctccatgtcg cccaagcgat
     5701 tgaagaaggt gcgaaacgcc accgacagtt cgcgacgggc gaggtgcgct ccgaggcaga
     5761 aatggggacc cggtccgccg aagccgacgt ggttgttcgg ggaccgacgc acgtcgaaac
     5821 gctcagggtc cgcaaactcg cgggggtcac ggttcgccgc gccgtacaac atgacgacct
     5881 tgtccccctc gtggaagtcg tgtcccgaca acgtcacctc acaggtcgcg gttcgcctca
     5941 tgaaggtgac cggtgagccc atccgcacga tctcctccac cgcggtcggg gtaacgccgt
     6001 cgatgtcgcc ttgccagatc gcccgctgat cggggttgcg ggtcaggaag tcgaccccgt
     6061 aggtgatggc gttgcgcgtc gtatcgttgc cggccaccgc caagaggatg aagaacggcg
     6121 ccagttcctc cggcgtcagc acgtcctcgc cgggctcagc gttcaccagc gccgaggtca
     6181 ggtcgtcctt cggattggcg cgacggtctc tcgcgagttc gccgagcagc accgacagtt
     6241 cggtgcccgc cccgatgagt gcggtgaaca gatcctcgcc ttcgccgacc gtgtcgggat
     6301 cgcccgcacc caagatgacg ttggtgcacc gcaacacgct gtcgaactcg ctgcggggaa
     6361 tgcccatcat gtcgcagatg atcagcagcg gaaacggctc gctgaacgcc gacaccaggt
     6421 cgacctcgcc ctgttcgcag aaccggtcga tcacctcatc ggcgacgtgt tgcaccgagt
     6481 cgaggaggcc ctgcaactgc cgcgggccga aattgcggct gacgatgccc cgctgtcgtg
     6541 cgtggcgcgg gtcgtccatc acgatgaagc tgccgaagaa ctccatgatg tcggtgggca
     6601 tgtcggcgat cgacaccgaa ccctgccccg aacagaactc cttgggacgc cgactgatct
     6661 cgatcacgtc gtcgtaacgg gtgctcagcc agtaccactc gttttccagc gtgagcgggt
     6721 tgggctggta ggtgtgcacg aacggtgcga tctcacggat ctccgcgaga cgcgcggctc
     6781 ggctctcgag cggttgagtc cagaactcaa tcgacgcaag ttcgtccgcc agggagtctc
     6841 cactcaagcc gaaaccggcc gatgatgaat cgttcatgtc gcgaccctac cgtgcaagtc
     6901 gccgctggtt acggtccgat gcgtcggccc tgcgcgtccc agtgagactc gaccttgcgc
     6961 gagggctgca cgcgtggggg ctcgcccggc atcttggggt aatccggcgg gaagttgagc
     7021 tcaccgccgg gcagggtctg ccagagctgc aacagcggat cgagcgaata ctcaacgtct
     7081 tccatctcgc gccacgggtc accctcggag gccagcaggt ccgggacggt gaggagattg
     7141 aactgcgtcg ggtcctccag gtcgccaagc cgatcccacg caatcggcat cgacaccggc
     7201 gcacccgccc gggcccggat gctgtaggcc ccggcgatcg tgcggtcgcg gttgttctgg
     7261 ttgaaatcga cgaagatccg cgacccgcga agctccttcc accaggcggt cgtcacgccg
     7321 ccgtcgcgcc gttcgagttc gcgtccgaac ccgatggccg cgtggcgcac gtcctcaaag
     7381 gaccagcgcg gttgaatccg cacgtagatg tgaatgccgc ggttcccact cgtcttcgcg
     7441 aagccgcgaa gacccagttc ctcgagaagc tcccgcgcca caccggccac ccgcacagca
     7501 tcggagaacc cggtacccgg ctgcgggtcg agatcgatgc ggaactcgtc gggatgatcg
     7561 acgtcgcggc ggcgcaccgg ccacgggtga aacgtcaagg tccccatctg tgcggcccac
     7621 acgaacgccg ccggctcggt cggacaaagc tcgtccgcgg ttcgaccgct aggaaaggtg
     7681 acccgcacgg tttcgatgaa ctcgggggct ccccgcggaa ggcgcttctg gtagaagccg
     7741 gcgccgtccg ggtcctgcgg gccgagcgcc aggcgcatcc cgtcacgcca gccgtcgggc
     7801 caacgctcga gcgcggtcgg ccgctccccc agcgaggcca tcatcgtctc gccgaccgca
     7861 gcgaagtact cgcacacctg caacttcgtg atggcgggcg tccggtcggt cgcctcgaac
     7921 acgatgcggt ccgggctcga cactcgaatc tcacgacccg ccgcgttcac caaccctgct
     7981 ggagccttgg ccatccaccg atgctacgac gtcgaccggc gagaccccgt cgatgccaag
     8041 atcggcgctg cggcaacgct cagtcgaccc gtgtgagcac ccggccgtag ccgccctgtg
     8101 ccccgaaccc ctcgacgaag gccccgtcgc tccacgccag ccttccgttg accagcacgt
     8161 gggtgacgca ttcgtcgtgt cgacgcacca accggtcgac cccgacgaca tcggccgagt
     8221 cgtcctcgac gatgttgtcg agccgctcat ccaacgttgc cgggtcgacg accgcgatgt
     8281 ccgcccggcc cccgaccacc agccgaccgg catcgatgcc gaggtagtcg gcgatttcgc
     8341 ccgtcatgcg atacaccgcc cgttcgggac tcagcaccgg ttcatcgtcg ccgccaccgc
     8401 catcgtcgcc gccaccaccg tcagcactcg ccgcgagccg caacagatgc aagggcaggt
     8461 cgtagaaggc catgttgcga aggtgcgctc cggaatcggc gaaccccatg atcgccgcag
     8521 ggtggttggc gatctgagcg accgagcgcg ggcggtcgtt cgcgacgacg gtgcgccacc
     8581 gcaactcgtg gccgaactgt gcctgcaggt ccatgaacgc ctcgatggca tcgaccccgc
     8641 gtttcgccgc cacctcaccg aaggtcttgc ccaccagggt ctcgtcgggg cagccgacga
     8701 tgaccggctc atcaagcttg cggtgatacg cacgcggtgc gagttggttg ttccactgct
     8761 tgcggaaccg ggcgcggaaa tccgggtccg acatcagccg tcgacgagcc tcgagatcgc
     8821 cgactgccaa agcctgggta ccggcgccga actcctccat caccggcacg tcgagcccgt
     8881 cgacgaacag gtcgaacacc agcggcaccg cctggaagcg cacgtcgccg cgcaggaccc
     8941 ggttgaacgc gttcgtggtg gcgccgatga accggtggct gccatgtgcc accaccggat
     9001 cgagcatcga caacaggttc atccgcagtg cagggcggcc gaccccggcc gacatcgcca
     9061 tgaggaccgg gatgttccag cgcccctgaa gatcggggac cacctgaaag atccgtcccc
     9121 gttcgcgcag caccttggcg aaggaccagt actcgccgaa agtcgcgaac accgacggcg
     9181 tcgggcgcga gttgaaccgg tcgccgccga gcttgtccca cggcagcgtg ttgaaactca
     9241 ttccgaggta cccggcgtcg agcgcctcgt cgagcaggcg caacatttcg cgccgttcgg
     9301 accgggtggg cttggccgaa gcgtcgacgg cgcgttccag acccatgacc gacgtgcgaa
     9361 cggccgaatg cccgagaaac agcgtgacgt tggggccgag tggcagcgac cgcaagtgcg
     9421 cgaggtaccc gctcgggtcg tcccagttct tggtggtttc caggagcggc aacaccacgt
     9481 cgcgcggcac gccttcgacg cggcagaact gatccgcgag gtcttcgggc tcgcccatca
     9541 cgaaggacag tccgcaactg ccgatgagca ccgtggtgac gccgtgtcgc accgactctc
     9601 cgaggctcgg ggcgatctcc aactcggcgt cgtagtgcgt gtgcaggtcg atgaagccgg
     9661 gggtcaccca acacccgtcg gcatcgacga ccgtcgctgc ggccggtgcg acgagcccga
     9721 gaccgatcgc cgccaccacc ccatcgcgca cgagcacgtc agcttccacc gaaggcgatc
     9781 cggccccgtc gaagacgcgg ccgttgcgga tgagaacggt tggggc
//
LOCUS       DAOOMA010000025         9684 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_8164_length_9684_cov_4.766746, whole genome shotgun sequence.
ACCESSION   DAOOMA010000025 DAOOMA010000000
VERSION     DAOOMA010000025.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 9684)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9684)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9684
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8164_length_9684_cov_4.766746"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            269..646
                     /locus_tag="PLP95_01485"
     CDS             269..646
                     /locus_tag="PLP95_01485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB44503.1"
                     /translation="MDDFRLACQRMMQSGFPSSGDNLVGMEMDVFILLGQLDAIDEDS
                     VEARTQPHAERLIVASCSPAATATIEEVVSGVDELWNSQLRYGYDAAHIWTAESAGPQ
                     LEFITQIAEGGFYVTGAVQIRER"
     gene            716..1072
                     /locus_tag="PLP95_01490"
     CDS             716..1072
                     /locus_tag="PLP95_01490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB44504.1"
                     /translation="MELEHVSLVDSFVLSIESDESYVAFELDAALETAHERFYEPPRP
                     GENGAYAHLRWCLRGEVWWNEGPHLDRPAIGADGERDFGGIDVWFSEGDVDHLEGEWG
                     EVAVRGAVQTVEYLSP"
     gene            complement(1189..1341)
                     /locus_tag="PLP95_01495"
     CDS             complement(1189..1341)
                     /locus_tag="PLP95_01495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB44505.1"
                     /translation="MSDLPFIGVEELERNSPDERRDSLGDRVVTDLDDLPGDFRHRVI
                     ATAESL"
     gene            complement(1391..1567)
                     /locus_tag="PLP95_01500"
     CDS             complement(1391..1567)
                     /locus_tag="PLP95_01500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB44506.1"
                     /translation="MASPSIPVDRAKVEDTLLTEFDWATADGAHERFKQLLNEMPRAK
                     ARFAEVLAELNDGR"
     gene            complement(1604..2020)
                     /locus_tag="PLP95_01505"
     CDS             complement(1604..2020)
                     /locus_tag="PLP95_01505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB44507.1"
                     /translation="MIVVDEYLAIRSLVGDTPSDLPDDVLAITTSVHWRILQRLHLPR
                     GGQLSQAMAALSEPGRAAFRRPAPEILEILDPRPLLDQAAEISAAYGGTGLLIAEMLT
                     ASLHHGRTLWYGSERNVGRRTRDIARDLGITVNVVT"
     gene            complement(2036..2230)
                     /locus_tag="PLP95_01510"
     CDS             complement(2036..2230)
                     /locus_tag="PLP95_01510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB44508.1"
                     /translation="MTKGEQLLADMRRARRSGDPRLDDADRAILRRLTSGDLADEFAE
                     ALAQDLADDDLLGGTSPDDK"
     gene            complement(2623..3381)
                     /locus_tag="PLP95_01515"
     CDS             complement(2623..3381)
                     /locus_tag="PLP95_01515"
                     /inference="COORDINATES: protein motif:HMM:NF019846.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HPB44509.1"
                     /translation="MTLDPRYDIIGHGYSTTRREDPRLRERIDIALGDSRTLVNVGAG
                     TGSYEPRDRHVIAIEPSDVMADQRSADLAPALRGTAATLPLRDLSVDAAMAVLTIHHW
                     DDQQERGVCELRRVARGPVVIVTYDPDVCAQMWLYRDYLAEAAALDRATFPPIDVLTS
                     WLGGSVCVEPILNSRDTPDWTLGSFWAHPERVLDGRARRATSAFARMEPAVVNRVVSA
                     VETDLRDGAWDRRNGELRDLADYDVGMRLIVAER"
     gene            complement(3421..4572)
                     /locus_tag="PLP95_01520"
     CDS             complement(3421..4572)
                     /locus_tag="PLP95_01520"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HPB44510.1"
                     /translation="MRGSKRKGRRPGTWELRVDAGRDPLTGSRQQKSVMFEGTSREAD
                     LALAELITKASRGGVSVRTRTVADAVEAGLQQAEFEGLEPTTVRNYRTAANNHVLPAL
                     GSKRLSHLTAEHLDRFYGALVEAGYSRSTVRGCHILVCRVLDQAQRWGWVAVNVGRDA
                     RPRRQTGPNPKPVPVDVMLTMLDAAAGTNPTLAMLIVLAADTGARRGELCALRWRHVD
                     FDARTLRIEAAIGETNVVYEKDTKNHQHRTVTLSEFAVEWLHEHWTRHAKACALCGTE
                     LADDAYVLAPETGGLTPWHPSNATRAFGRLRDRLELPTWVHLHGLRHLQVTQLLDAGI
                     PLRSVSGRVGHLNMSTTGNIYAHWIQESDTRSAQVVEDRIWRRSADGQG"
     gene            complement(4710..4783)
                     /locus_tag="PLP95_01525"
     tRNA            complement(4710..4783)
                     /locus_tag="PLP95_01525"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4747..4749),aa:Thr,seq:tgt)
     gene            4890..5696
                     /locus_tag="PLP95_01530"
     CDS             4890..5696
                     /locus_tag="PLP95_01530"
                     /inference="COORDINATES: protein motif:HMM:NF024258.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HPB44511.1"
                     /translation="MTLVAQISDPHICRRGELLYGIVDSATALRRAVSTVNALNPQPD
                     LVIVSGDLANDGAAEAYDHLIEILDELHRPWLAIPGNHDVPEVAAEVLVDHCFPQDDG
                     GWGPCSGVVDLDGVRIIGLNSVCEGEPSGVLDPRDLEATAVALAGRGDRIAVVVVHHP
                     PVALGLAAMDAMRLDDASARRLGDLVDDHAVDTVLVGHVHRASATGWRGSLLLSCPSP
                     AFSIAMDLDPATPLTVSDEPAAILVHRFIDGVHTAHVHAVGERLSIAVNY"
     gene            5884..7353
                     /locus_tag="PLP95_01535"
     CDS             5884..7353
                     /locus_tag="PLP95_01535"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HPB44512.1"
                     /translation="MERLTRLAAVALGVPVVIVELLDSDRTLCVATVGTSVRELELDR
                     SPCAALVRSGGEQLVIEAAEASVAQGAQRPGLIDAALSIRSFATRLLRIDGRIVGAVT
                     VAAPDPRDFDTDDRIALCDLAGVVEAHFETTVAAWSGERQLAATTGPNHGLNPALDPH
                     LDVVAPGEPVREPVAAAESVVASAVEASALEALVRRQAVMTQHSADVVIVIAPDGKVA
                     SQSPSLGRMLGHPEPFTAPGGALSIVHAQDREFARAQIRDVVDRNAIGDPFVVRVLAG
                     NGTIRRMECVAASYANDPAVGGTVLTMHDITDRYQLSQLVSFQATHDALTELPNRHLL
                     AERVTPAIARFRRTGSQIAVISFEIVGFQQLADQLGRDAGDQLLRCCAKVLHRSIRAG
                     DCASRLGGAEFAVLLDPVAHSDVVVEVADRIAAAMCGPHSLSTGISTCEVTVGIATGQ
                     PDDDAERIISRAARGRSRRSTAKPLVEIADEPASGSDGL"
     gene            complement(7339..7704)
                     /locus_tag="PLP95_01540"
     CDS             complement(7339..7704)
                     /locus_tag="PLP95_01540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB44513.1"
                     /translation="MSFNADTAGGPRDGLVCYYGEPEWTNARFTLVDVMDNLGGTGAL
                     VIARQQNGSVETRRVDFEDITGLDELTDGMDSESLAGYQRLELSVNRSIYCQIIWPQA
                     STERLLASLTHSNRQSQTV"
     gene            7806..8240
                     /locus_tag="PLP95_01545"
     CDS             7806..8240
                     /locus_tag="PLP95_01545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB44514.1"
                     /translation="MSSDAATLDLTDFDAMGIAADIIVAVRAHALQHEVSTTAEVSAP
                     QGWHRVVVNCSPTGNVNLRVRFVDLTTSRANNVTKALVARDWLIDEDCDGASTRFLPG
                     AEAASIAFEVLAVLSLAGAPGDRRTVTAIDSAGAEIALGPTN"
     gene            8320..8877
                     /locus_tag="PLP95_01550"
     CDS             8320..8877
                     /locus_tag="PLP95_01550"
                     /inference="COORDINATES: protein motif:HMM:NF024791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytR C-terminal domain-containing protein"
                     /protein_id="HPB44515.1"
                     /translation="MATQQRRRPARAPQTPQNSTQLGVILVVVAVVVALLLFTVGGGS
                     DSSDDDLTAADQVQQGATTTSTPEVETVTTTPPAALKLVVANGSSVKGRAGATAERFN
                     GLGYAASAVDGTSTATTVIYYVEGMEADGVAVAGVMGFGPDRTALMPAAQPLQTPDPA
                     AQVIVLIGPDFDPGTAAWGAATATN"
     gene            8922..>9684
                     /locus_tag="PLP95_01555"
     CDS             8922..>9684
                     /locus_tag="PLP95_01555"
                     /inference="COORDINATES: protein motif:HMM:NF014634.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0031388 - organic acid phosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HPB44516.1"
                     /translation="MRVVIAPDKFRSTATAAQVAASIEAAVVACGHEAVLKPLADGGE
                     GTLDAIGGSNRVTVVSGPLGDDVAARWRLDGRTAWIEMAQAAGLALVGGADRNDAVAA
                     SSVGVGELITAALEAGATRIVVCVGGSATTDGGFGAVRELLVGRLSGVELTVACDVRT
                     TFVDAARRFAPQKGASAAEVRLLERRLERLAGLYTDEYGVDVRDMVGSGAAGGLAGGL
                     AAVGATLVSGSEVVAEEVEADEALVGADLIVTGEGF"
BASE COUNT         1555 a         3037 c         3401 g         1691 t
ORIGIN      
        1 cacacgtacg gagattcgcg gatccccgaa acattcagga tcgcgaccta ggcccgccgc
       61 ttagggcaat ccgtgtggag cccgaactcg gattcgaacc gaggacctgc tgtttacaag
      121 ccgcgattcg aatgtccatc aagtgccgcc agcgtccgtc ccgtcacggg tctcgcacca
      181 acgtctcacg tacaataccg cccttatccg ggggtgtccg ccacgttggt gagacaaatg
      241 gtgagacgct gcccctacgg tctccctcgt ggatgacttc cgtctggcgt gccagcgaat
      301 gatgcagtcg ggcttcccca gcagcggcga caacctcgtc ggcatggaga tggacgtgtt
      361 catcttgctc ggtcaactcg atgcgatcga cgaggacagc gtcgaggcga ggacacagcc
      421 ccacgccgag cgcttgatag tggcgagctg ttcgccggca gcgacggcca cgatcgagga
      481 ggtcgtgagc ggtgtcgatg aactctggaa cagtcagctc cggtacgggt acgacgccgc
      541 ccacatttgg accgccgagt cggcgggccc acagctggag ttcatcaccc agatcgctga
      601 gggcggcttc tacgtgaccg gagcggtgca gatcagagag cggtaggtgc atcagcgcac
      661 ttgggtgagc gagggggcga ctcgtgtgct cggacggcgt gcaaggcttg gcgtgatgga
      721 actcgagcat gtgagcctgg tcgacagctt cgttctcagc atcgagtccg atgagtccta
      781 tgtcgccttc gagttggatg ccgctctcga gaccgcccac gagaggttct acgagccacc
      841 tcggcccggt gagaacgggg catacgcgca tctccgttgg tgcctacggg gcgaggtgtg
      901 gtggaacgag ggcccgcatc tcgatcggcc ggccatcggc gccgatggtg aacgggactt
      961 cggtgggatc gatgtctggt tcagcgaggg tgacgtggac caccttgagg gggagtgggg
     1021 tgaagtagct gtccggggcg cggtccagac cgtcgagtac ctgtcgccgt gaagcgtcag
     1081 cttgttccgc ttgcggaaca agccagtctg tgccacttcc tgaccacgtc gaatgaccac
     1141 cgattcgaga ctgcgcggcg cggtccaggc cgacgtcgcc ggccggcgtc agagcgactc
     1201 agcagttgcg atgacgcggt gacggaagtc gccggggaga tcgtcgaggt cggtgacgac
     1261 acgatcgccc agagaatccc gcctctcgtc aggcgaattg cgttcgagct cttcgacacc
     1321 gatgaagggc agatcgctca ctggctcgac ggtacccgcc cggccgtcgg cccggcaggc
     1381 gggtcacgcc tcagcggccg tcgttgagct cggcgagcac ctcggcgaat cgcgctttcg
     1441 cacggggcat ctcgtttagg agctgcttga atcgctcgtg ggcgccgtcg gccgtcgccc
     1501 agtcgaactc ggtcaggagt gtgtcctcga ccttcgcacg gtcgacggga atggaaggcg
     1561 aggccacgtc gagatcctag acagagcgcg aaccgaatgg ccgctacgtg acgacgttga
     1621 cggtgatccc gagatcccgg gcgatgtcgc gggttcgtcg cccgacgttg cgttcgctgc
     1681 cgtaccagag cgtccggccg tggtggagac tcgccgtaag catctcggcg atcagcagcc
     1741 cagtcccgcc gtaggccgct gagatctcgg cggcctggtc gaggagcggt cgtgggtcga
     1801 ggatctcgag gatctccggt gctggtcggc ggaaggccgc acggccgggc tcggagagcg
     1861 ccgccatggc ttgggagagc tggccgccgc ggggtaagtg gaggcgttgc aggattcgcc
     1921 agtggaccga ggtggtgatg gcgaggacgt cgtcgggaag gtcgctcggc gtgtcgccga
     1981 cgagtgagcg gatcgcgagg tattcgtcga cgacgatcaa cgggcttgaa gaccgctact
     2041 tgtcgtccgg cgaggtgccg ccgaggagat cgtcgtcggc caggtcctga gcgagcgcct
     2101 cggcgaactc atcggccagg tcgccggagg tgagtcggcg gaggatcgct cggtcggcgt
     2161 cgtcgaggcg agggtcgccg ctgcgtcgcg ctcggcgcat gtccgcaagg agttgctcgc
     2221 ctttcgtcat cgctccattg tgccctgcgt cgcgcccgaa ggcgacgcac gttgttgctc
     2281 gctcgatctc ggcgtgatca cggtggtcag gagacggcac agactggctt gttgcgcaag
     2341 cgcaacgagc tgggagatgg tggccgcctg gcgccaccac agctacatgt tggtcgcacg
     2401 gtcgttcagc tgtggtcgcc gtcgacttgg tcggagaacc gaccgcactt gaggaacaca
     2461 actcgtgagg catccggcaa cgttcgcccg gttggcgaaa gcccggctcc ggcgtcacgg
     2521 gtgtgggtcg aagattcgcg ggcactcgtc cgaagctcat cggataaggg tcgaacccga
     2581 gcgcagcgcg ctcccgtggt cgaggtgtga cccggcgtct ggtcagcgct ctgcgacgat
     2641 cagccgcatc ccgacgtcgt agtccgcaag atcgcgaagc tcgccgttgc gtcgatccca
     2701 cgctccgtcc cggagatctg tctcgacagc agaaacgaca cggttcacta ccgctggttc
     2761 catacgtgcg aatgcggatg ttgccctgcg cgctcgcccg tcgaggacac gttcagggtg
     2821 agcccagaac gaaccgaggg tccagtcggg cgtgtctcgg gagttgagga tcggttcgac
     2881 gcagacggaa ccaccgagcc agctcgtgag cacgtcgatt ggcggaaatg ttgctcggtc
     2941 gagtgctgcg gcttcggcga ggtaatcgcg gtacagccac atctgggcac aaacatccgg
     3001 gtcgtaggtc acgatgacga ccgggccacg ggcaacgcgg cggagttcgc aaacgccgcg
     3061 ctcttgttga tcatcccagt ggtgaatcgt caggacagcc atcgctgcat caacgctgag
     3121 gtctcgcagg ggaagcgtgg ctgcggttcc ccgcaatgct ggcgcgaggt cggctgatcg
     3181 ttgatctgcc atgacgtcgc tgggctcaat tgcgatcaca tggcggtcgc gtggctcgta
     3241 cgaacctgtc cccgcgccga cattcacgag cgtccggctg tcgccgaggg cgatgtcgat
     3301 gcgctcacgc agtcgagggt cctcgcgacg ggtggtcgag tagccgtgtc cgatgatgtc
     3361 atagcgcgga tccagggtca cgcgcttgag gttacaagcg ccgcttacgc gagctgatgg
     3421 tcacccctga ccatcggccg accgtcgcca gattcggtct tcgactactt gggcggagcg
     3481 ggtgtcggat tcttggatcc agtgggcata gatgttgccc gtggtcgaca tgttcaggtg
     3541 accgactcga ccggagacgc tacgcagcgg gatgccggcg tcgaggagct gggtgacctg
     3601 gaggtggcgg agcccatgga ggtgtaccca cgtcggtagc tccaatcggt cgcgcagccg
     3661 gccaaacgct cgggtggcgt tcgatggatg ccacggcgtg agaccaccgg tttcgggggc
     3721 gaggacgtac gcgtcgtcgg ccagctcggt gccgcagagt gcgcacgctt tggcgtggcg
     3781 ggtccagtgc tcgtgcagcc actcgacggc gaactccgag agcgtgacgg ttcggtgctg
     3841 gtggttcttg gtgtccttct cgtagacgac gttcgtctcg ccgatcgccg cctcgatccg
     3901 gagcgttctc gcgtcgaagt cgacgtgccg ccaccgcaac gcacacagct caccccgccg
     3961 ggcgccggtg tcggcggcca gcacgatcag catcgccagc gtcgggttcg tcccggcagc
     4021 agcgtcgagc atcgtgagca tcacgtcgac cgggaccggt ttcgggtttg gccctgtttg
     4081 acgtcgcggc cgggcgtccc gtccgacgtt gaccgccacc catccccacc gctgcgcctg
     4141 gtcaaggaca cggcacacga ggatgtgaca gccacggacg gtggagcggg agtacccggc
     4201 ctctaccagg gcgccgtaga agcggtcgag gtgctcggcg gtgagatgcg acaagcgttt
     4261 cgatccgagg gcggggagga cgtggttgtt cgctgcggtc cggtagttgc gcacggtggt
     4321 cggctcgagc ccttcgaact cggcctgttg tagcccggct tcgacagcgt cggcgacggt
     4381 gcgggtccgg actgacacgc cgccccggct ggcctttgta atcagttccg ccagcgcgag
     4441 gtcggcctcg cgtgaggtgc cctcgaacat gacggacttc tgttgacggc tgccggtcaa
     4501 cgggtcgcgc ccggcgtcga cgcggagttc ccatgtgccg ggccgtcggc cttttcgttt
     4561 gctgcctcgc atcccgcgtc ctccgtgtct caccgatccg tgagacgtcg gtgagattcc
     4621 gtgggacacg gtgacgggac cgtctgaaca cgagtctgac catgcggcca aaaccccgag
     4681 aaatatggcg atggtcaggg gtgaccagga gcccgaactc ggattcgaac cgaggacctg
     4741 ctgtttacaa gacagctgct ctggccagct gagctattcg ggctaacggg tggcgccgcg
     4801 ggtgggtcgg tgccggggga aagtgtatcc gcgcccgcgg ttcttcccga ctcggaatcg
     4861 atcgatcgcc acggcgtact ctgggtccga tgacgctcgt agcgcagatc tccgacccgc
     4921 acatctgtcg acgtggcgaa ctcctctacg gcatcgtcga ttccgccacc gcgttgcggc
     4981 gagcggtttc gacggtcaac gcattgaacc cccagcccga cctggtcatc gtgagcggcg
     5041 acctggcaaa cgacggtgcg gccgaggcct atgaccatct catcgagatt ctcgacgagc
     5101 tccatcgacc atggctggcc atcccgggca atcacgacgt gccggaggtc gccgcggagg
     5161 tgctcgtcga ccactgcttc ccccaggatg acggcggatg ggggccatgt agcggtgtgg
     5221 tcgacctcga cggggtgagg atcatcgggc tcaactcggt ctgcgaaggc gaaccctcag
     5281 gtgtgctcga cccgcgcgat ctcgaggcga ccgccgtcgc gttggccggg cgcggcgacc
     5341 gcattgcggt cgtggtggtg catcatccgc cggtcgcact cggtcttgcg gcgatggacg
     5401 ccatgaggct cgacgacgcc tcggcacggc gcctcggcga cctcgtcgat gaccacgccg
     5461 tggacacggt gctcgtcggt cacgtacacc gtgcctcggc gaccggctgg cgcggctcgc
     5521 tgttgctgag ttgcccgagc cccgcctttt cgatcgctat ggatctcgac ccggccactc
     5581 cgttgacggt gtccgatgag ccggcggcga ttcttgtaca tcgctttatc gacggcgtgc
     5641 acacggctca cgttcatgcg gtgggcgagc gcctgagcat cgcggtcaac tactgactcg
     5701 acggtgaggg cccctccacc cgtggaccgc aggggagatt cgcgggtgcg gggagatcgc
     5761 tcagaaaggc tcggtttcgg tcgataagga gatgtgagca cagcgcagac ttctgatgac
     5821 gagttcgaac ggctgcgagt cgttcggcag ctccaggcgg tgggcggccc gaccgatcca
     5881 acgatggaac gcctgacacg ccttgccgcg gtcgccctgg gggttcccgt cgtgatcgtc
     5941 gagttgctcg actcggaccg aacgctgtgt gtggcgaccg tcggaacctc ggttcgcgaa
     6001 cttgaactcg accgttctcc ctgcgccgcg ttggtccgtt cgggaggcga acagctggtc
     6061 atcgaggcgg ccgaggccag cgtcgctcag ggtgcgcagc gtccggggct catcgacgcc
     6121 gcgttgtcga tccgttcgtt cgccactcgc ctgttgcgaa tcgacggtcg catcgtcggg
     6181 gcggtcaccg tcgccgcccc cgacccccgc gacttcgaca ccgacgatcg aatcgcgctc
     6241 tgcgatctcg ccggggttgt cgaggcgcat ttcgaaacga cggtggcggc ttggtctggc
     6301 gaacgacaac tcgccgcaac gacagggccg aaccacgggc tgaacccggc gctcgatccg
     6361 catctcgatg tcgttgcgcc gggcgaaccc gttcgcgaac cggtcgcagc ggcggaatcg
     6421 gtggtcgcga gcgcggtcga ggcgagtgcg ctcgaagcgc tggtgcgtcg acaggccgtg
     6481 atgacccaac acagtgccga cgtggtcatc gtcatcgctc ccgacggcaa ggttgcctcg
     6541 cagagcccgt cgctgggccg aatgctcggg catcccgagc cgttcacggc tccgggtggg
     6601 gcgttgagca tcgtgcacgc gcaggaccgg gagttcgccc gagcccaaat ccgcgatgtc
     6661 gtcgaccgca acgcgatcgg cgatccgttc gtggtgcgag tgctcgcggg caacggcacg
     6721 attcgccgca tggaatgtgt cgctgccagc tacgcgaacg acccggccgt cggtggaacg
     6781 gtgctcacga tgcacgacat caccgatcgc taccaactct cgcaactggt gagctttcag
     6841 gcgacccacg acgcgctcac cgagttgccg aaccgccacc tgctggcgga acgggtgacc
     6901 ccggccatcg cacgctttcg gcgaaccggg tcgcagatcg cggtgatcag ctttgaaatc
     6961 gtagggttcc aacagctcgc cgatcagctg ggacgcgatg cgggcgacca gctgttgcgc
     7021 tgctgcgcga aggtgcttca ccgctcgatt cgcgcgggtg actgtgcgtc gcggctcggc
     7081 ggcgccgagt tcgctgtcct cctggatccg gttgcccaca gcgacgtcgt ggtcgaggtc
     7141 gctgatcgga ttgccgcggc catgtgcggg ccgcactcgt tgagcaccgg gatctcgacc
     7201 tgtgaggtca cggtgggaat cgcgacggga cagcccgacg acgacgccga acgcatcatt
     7261 tcccgagccg cgcggggtcg cagccggcga tcgaccgcga agccgctcgt ggagatcgcc
     7321 gacgaaccgg cctccggatc agacggtctg tgactgtcga ttcgagtggg tcagcgaggc
     7381 gagcaaccgc tccgtcgacg cttgcggcca gatgatctgg cagtagatcg agcggttgac
     7441 gctgagctcg agacgttggt acccggcgag ggactcagaa tccataccgt cggtgagctc
     7501 gtcgagaccg gtgatgtcct cgaaatcgac gcgtcgggtc tcgaccgatc cgttctgctg
     7561 gcgcgcgatg accaacgctc ctgttccgcc gagattgtcc atgacatcga cgagggtgaa
     7621 cctggcattg gtccactcgg gttctccgta ataacagacg agcccgtctc gaggcccgcc
     7681 ggcggtgtct gcattaaatg acatgggaga caccctaatt gtctccactg acagtgaagc
     7741 caaaccggag cgcaacgacc cggaatgtcg ccatcgctcg gtcgcggctg tgtcaggctg
     7801 caaccatgtc gtccgacgcc gcaaccctcg acctgaccga cttcgacgcg atgggaatcg
     7861 ccgccgacat catcgtcgcg gtccgcgccc acgcactcca acacgaggtc agcaccaccg
     7921 cagaagtgtc cgctccgcag ggctggcacc gcgtcgtggt caactgcagc ccgactggta
     7981 acgtgaatct gcgggtgcgc tttgtcgatc tgaccacctc gcgggccaac aacgtgacca
     8041 aggcgctggt cgcccgcgac tggctgatcg acgaagactg cgacggcgcg agcacccggt
     8101 tcctgccggg tgccgaggcg gcgtcgatcg ccttcgaggt tctggcggtg ttgtcgctgg
     8161 cgggggcccc tggcgaccga cgcacggtca ctgcgatcga cagcgcggga gcggagatcg
     8221 cgctggggcc gacgaactga cccggtcgac ggggccgcgg cggctagggt ttgacgcaat
     8281 tgaccgttgc gtccgacgcc gatttcccga ggagtacgaa tggcgactca gcagcgacgc
     8341 cgcccggcac gtgcgcccca gactccacag aattccacgc agctgggggt gatcctcgtc
     8401 gtcgtcgcag tggtggtggc tttgctgttg ttcaccgtgg gcggtggttc ggattcctcc
     8461 gatgacgatc tcacggcggc ggaccaggtg cagcagggcg cgacgaccac gagcacgccg
     8521 gaggtcgaaa ccgtgacgac caccccgccg gcagcactca agttggtcgt ggccaacggt
     8581 tcgagtgtga agggtcgagc cggagcgacg gcggagcgct tcaacggttt gggatatgcg
     8641 gcgtcagcgg tcgacgggac ctcgacggcg accacggtga tctattacgt cgaaggcatg
     8701 gaggccgacg gggttgccgt cgctggtgtg atggggttcg gccccgaccg cacggcgctg
     8761 atgcccgccg cccaacccct tcagaccccg gaccccgcag cacaggtgat cgtgttgatc
     8821 ggccccgact tcgatccggg caccgccgca tggggcgcgg cgaccgcgac gaactgatcg
     8881 tcggcgacgc gggctgagtc gaccggtagc cgttcgacct catgcgagtc gtcatcgccc
     8941 ccgacaagtt ccgctcgaca gcgaccgcag cgcaggttgc ggcgtcgatc gaggcggccg
     9001 tggtcgcctg cggtcacgag gcggtgctca agccgttggc cgacggtggt gaaggcaccc
     9061 tcgacgcgat cggcgggtcg aaccgggtga cggtggtgtc ggggccgctc ggagacgatg
     9121 tcgcggcccg gtggcgcctc gacggtcgca ccgcctggat cgagatggcc caggcggccg
     9181 ggttggcgct ggtcggtggg gccgaccgaa acgatgcggt cgccgcgtcg agcgtcgggg
     9241 tcggagagct catcaccgcc gcgctcgagg cgggagcgac acgcatcgtg gtgtgtgtcg
     9301 gcgggtcggc gacgaccgac ggcggcttcg gagcggtccg cgaactgctg gtcggtcggc
     9361 tcagcggagt cgagctcacc gtcgcgtgtg acgtgcggac caccttcgtc gatgcggcgc
     9421 ggcggttcgc tccacaaaag ggcgcgagcg cggccgaggt gcggctgctc gaacggcgct
     9481 tggaacgcct cgccggcctc tacaccgacg agtacggcgt cgatgttcga gacatggtcg
     9541 gcagcggggc ggccggcggg ctggcgggcg ggttggcggc ggtgggggcg acgctcgtga
     9601 gcggatccga ggtggtcgcc gaggaggtcg aggccgacga ggcgctggtc ggcgccgacc
     9661 tgatcgtgac cggcgaaggg ttcg
//
LOCUS       DAOOMA010000026         9398 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_8524_length_9398_cov_4.250883, whole genome shotgun sequence.
ACCESSION   DAOOMA010000026 DAOOMA010000000
VERSION     DAOOMA010000026.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 9398)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9398)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9398
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8524_length_9398_cov_4.250883"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            3..8915
                     /locus_tag="PLP95_01560"
     CDS             3..8915
                     /locus_tag="PLP95_01560"
                     /inference="COORDINATES: protein motif:HMM:NF019953.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004318 - enoyl-[acyl-carrier-protein]
                     reductase (NADH) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1729 domain-containing protein"
                     /protein_id="HPB44517.1"
                     /translation="MGHSQGLLAAAAATGRLDEVDALALAHLIGTSVARHAVRTGLVA
                     NGSEHTPMLAVSGITEAQCLDLLDEFNAACGPDSPDRVVLGVVNGTARLVLVGRPERL
                     AAVQRRLATHPALDGPAGRAAPRFDPLEVAAGFHHPALAIAVDEVVEAADSIGISLAD
                     SSCADASGCPMNVLGPASILRHLVEAIVSTPFSWIDALAVPADDQIRWLVDLGPDSSA
                     GQLARAHLAGTGVGVCGVVATGGVSPLLTPGAAPSIDGRFAAFAPTLRRLPDDTLAVD
                     TKLTRLTGQSPFILAGMTPTTVDATIVAAAANAGHWAELAGGGQVTTAILTERLRELD
                     ELLDEGIAVHFNTLYLDPYLWGLHFGETGAVVEALRRGAPIRGVTVSAGIPDLDEAVA
                     LVERLGGLGVETVTFKPGTVDGIRRVVAIADAVAPTPIVAHVEGGLAGGHHSWEQLDE
                     LLLATYADLRRADNLVVCVGGGIGAPDEAVAYLDGSWAHRHGRPTMPLDGILIGTAAM
                     ACAEATTSPQVKRLLVDAPGTASFISTGRVAGAVTSGRSQLGADLHEIDNAASRAGRL
                     LDEVAGDGEAAIRRRAEIIEAIDATAKPYFGELAEMTYGQVLDRFVQLCAIGRGGRYE
                     DGAWLDRSWRSRFLELLHRVEARLCASERGTIPTVFGDERDLDDPGAALTIIRAHHRE
                     VDSTVLHPADVSWFVELCRRPGKPVPFVPIIDAEVRRWWRSDSLWQAHDDRYDADGVI
                     VIPGPAALRAVRAVDEPVAALFNRFEAATVAALVEAGVEPVDAAQRRETHTDHRDRRG
                     DDPISRLLAEPNVEWTTAGPDGLRRRRRPNPALAVAPATAWHLDVSGNGHGPASVARA
                     HRSDAVLVATGPNSVRLDVPLTVHRDTMSTASIEFDLSSDGRSAAVVSPRSSAAWSVA
                     LRCASVPVHGPDPTPDQTSATGTEFLLATALDHAALFGAASPLPDALVGPCWSAVFAA
                     LEREFDVSSGGFADLLHLDHHLELHGGSMPTRPALPDTDGNLDIDVRVESNTATSAGR
                     AVAVRIELANAHAKVATLHERFLIRSDIGKEPLGAPRALRLADDAAPTPRRTLADIEV
                     NTPPNGIAMASFTGDHNPLHLSREVAAVAGFTAPIVHGMWVSAAMQQAARRSAAGTHG
                     GTGVEVREWHANFVAPIPHDSPVSISVVRVARIDGGDVVEVQATVDGDLVATAAGVLS
                     APRTVYAFPGQGIQRVAMGLDALTRSAAARAVWERADHHTRSALGFSIRHVVRDNPVE
                     LVVDGERFRHPDGVLFLTQFTQVAMATLASAQIAELREAGGFVPDAIACGHSVGEYNA
                     LAAVTGILALEDVLEVVYQRGLAMHHLVPRDAAGNSRYRMAAVRPSEAGLSESELREL
                     VADLARDTDPEEFIQIVNFNLRGRQYAVAGTQAPLRRLHDELERRRAERNGKGVYVEV
                     PGIDVPFHSAVLHHGVAGFRTKLDAILPDRIDPTLLVGRYIPNLVPRLFSLCRDFVVE
                     VAEVAEAPALNSVIERWDDWSQRPEALCRVLLLELLAWQFASPVRWIETQDLVFALDS
                     GIERFVEVGLESQPTISSLARQTLSGEPGRYAAVTVANLEADRAELFGEDTDPPFDEL
                     AEAADDGRERAGEAGEAGAEEPPSPSAPTAPAGATSAASAAGAAVAPEDLAWTPADGL
                     RFMLSWWTKVRIDQLADDDTIESLVDGASSRRNQLLMDIGKEFGLGAIDGAADAALST
                     LIDEVAARARTYRAPGPVLRPTIDEQLRRVTGPARSRPRAIDERIANHWGLGAGWTTV
                     VAAAVAAGTRAGTSVRGGDLATLSPTEPASAEDLDGILDAAVTAVARASGVEVAPLVA
                     PGGSDALDAATATALLDTIIGADGVLTEIGRVITRRTSDAPTGDSSFDLQETQRLHDI
                     AAAVTRELGAGWQAAVAPAFDPARTLVIDDRWAGQREDLARLANSDPDPDRDRTDRER
                     RFARLGRGLAPSAIGHARWHEREARRRGDVDLADRLARLVAESAATAVRTDDLSDDVA
                     LVTGASPGSIAAGIVAELLGRGATVIATASSLTSERIAFFRELYHDHAHHRASLWLAP
                     ANLASFSDVDRLVEWLGTDVDEATGGEPRIVKPALTPTLVFPFAAPAVRGDLSEVGPS
                     AELEMRVLLWSVERLIAALAEPDSGRDPATRLHVVLPGSPNRGTFGGDGAYGEAKAAL
                     DALTTRQAHEPWGERVSIVHAIIGWVRGTGLMSGNDALVPLVETEGIGTWSAEEIARE
                     LVDLGDRDHRHASGGALTVDLSRGLWNSTIDLADLAAQARDLASAADIAAVEPAAPDS
                     PVLIDALPHPPSAPAPTGIDWPALDLTPADTVVIVGFGEVGPLGSARTRFDVEVTDSL
                     SAGAVAELAWMCGLIRWESTPTPGFYDVESGELVAEHLIAERYEQRLLDNSGVRFLDD
                     DGAQVDGTAPLLASVFLERDLSFVVESEAQAEAFVAADPDRTSATPNADGTFTVTRSA
                     GTEIRVPRRTVLPRRVAAQLPAGFDPSVYGIPSDMIEALDRVAVWSLVATVDAFVDAG
                     IEPAELLSWVHPTEVANTQGTGIGGISAIRRMYVDSLLGDEAPNDILQEALPNVVAAH
                     VMQSYVGGYGATIHPVAACATAAVSLEEAFDKIRSGRAAVVVAGGFDDFGHEGLIGFA
                     AMNATASSDAMEAAGIEARRMSRANDRRRAGFVEGQGGGTVVVARGDIAYELGLPVRG
                     VIAWAGSFSDGINTSIPAPGLGLIGAVRGGGASPLARALRSLGLDADDIAVVSKHDTS
                     TAANDPNESLIHERLSSALGRHEDNPLYVISQKTVTGHAKGGAAAFQTNGLCQVLESG
                     AIPPNRSLDCVDGDLERYARLVWPTEPLHHRGSLRAGLLTSLGFGHVAAIVAIVHPEA
                     FVATIEPDRRDDYRRRAAQRSERGQRRRVAARVGGAPLYVRPDHRLGDRPAARRDLEA
                     AMLVDPDARLGPDGTYRVPTIDQAAS"
     gene            8912..9316
                     /locus_tag="PLP95_01565"
     CDS             8912..9316
                     /locus_tag="PLP95_01565"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: protein motif:HMM:NF013786.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HPB44518.1"
                     /translation="MIAGVGVDVVAVSGFRDQLELDATTFTEVFTPAERDLRATGRGD
                     AEHLAARWAAKEAFVKAWAGSRHGRPPQLQSVEMRDIEVVCDAWGRPALRLHGEVDEE
                     FSRSVPDASVHLSISHDGDVAIAYVVIDRVMP"
BASE COUNT         1380 a         3468 c         3169 g         1381 t
ORIGIN      
        1 cgatcggtca cagccaggga ctgctcgccg cggcggccgc caccgggcga ctcgacgagg
       61 tggacgccct cgcgctcgca cacctcatcg gaacctccgt cgcccgccac gccgtgcgca
      121 cagggctcgt cgccaacggt tcagaacaca cgccgatgct cgcggtcagc ggtatcaccg
      181 aggcgcagtg tctcgatctc ctcgacgagt tcaacgccgc ctgcggcccc gactcccccg
      241 accgcgtcgt gctcggcgtc gtcaacggaa ccgctcggct ggtcctcgtc ggacgccccg
      301 agcgcctcgc cgccgtgcag cggcgactcg cgacgcaccc ggcactcgac gggccggccg
      361 gtcgcgccgc cccgcgattc gacccgctcg aggtcgcggc gggattccac catcccgcac
      421 tcgcgatcgc ggtcgacgaa gtcgtcgagg cggccgactc gatcggcatc tcgctcgccg
      481 actcgtcgtg tgccgacgcc tccggctgcc ctatgaacgt gctcgggcca gcgtccatcc
      541 ttcgacacct cgtggaggcg atcgtctcca ccccgttctc atggatcgac gcgctcgcgg
      601 ttccggccga cgatcagatc cggtggctgg tcgacctcgg acccgactcc tcggcggggc
      661 agctcgcacg cgcacacctc gcaggcaccg gggtcggagt ttgcggcgtc gtggcgaccg
      721 gaggggtgtc accgttgctc acccccggcg ccgctccgag catcgacggt cgattcgcgg
      781 ccttcgcacc gacgctgcgg cgcctgcccg acgacaccct cgcggtcgac accaagctga
      841 cccggctcac cggacagtcg ccgttcatcc tcgccgggat gacccccacc accgtcgacg
      901 caaccatcgt cgccgccgcc gccaacgccg gacactgggc ggaactcgcc ggcggcggac
      961 aggtcaccac cgcaatcctc accgagcgcc tccgagaact cgatgaactg ctcgacgagg
     1021 gaatcgccgt gcatttcaac accctgtatc tcgatccgta cctgtggggg ctgcacttcg
     1081 gcgagaccgg cgccgtcgtc gaggcgttgc gccgcggcgc gccgattcgg ggcgtcacgg
     1141 tgtcggccgg catccccgac ctcgacgagg ccgtcgcact cgtcgaacgg ctcggcggcc
     1201 tcggggtcga gaccgtcacc ttcaaaccgg gcaccgtcga cggcatccgc cgggtcgtcg
     1261 cgatcgccga cgccgtggca ccgacaccca tcgtggccca cgtcgaaggg ggcctcgcgg
     1321 gtggccacca cagctgggaa caactcgacg aactcctcct ggccacctac gccgacctgc
     1381 gccgggccga caacctcgtg gtgtgcgtcg gtggcggaat cggcgcaccg gacgaggccg
     1441 tcgcctacct cgacggttcg tgggcccacc gccacgggcg cccgaccatg ccgctcgacg
     1501 gaatcttgat cggaaccgcg gccatggcct gcgccgaggc gacgacctcg ccgcaggtca
     1561 agcggctact cgtcgacgcc ccgggtaccg cgtccttcat ctccaccggt cgggtcgccg
     1621 gagccgtcac gtcggggcgc agccagctcg gcgccgacct gcacgagatc gacaacgcag
     1681 cgtcgcgcgc cgggcgtctg ctcgacgagg tcgccggtga cggcgaggcc gcaatccgtc
     1741 gacgggccga gatcatcgag gccatcgacg ctacggcgaa gccgtacttc ggcgagttgg
     1801 ccgagatgac ctacgggcag gtgctcgacc ggttcgtcca gctgtgcgcc atcggccgcg
     1861 gcgggcgtta cgaggacgga gcctggttgg atcggtcgtg gcgatcacga ttcctggaac
     1921 tgctgcatcg ggtcgaggcc cgcctgtgtg cctccgagcg cggcacgatc ccgacggtgt
     1981 tcggcgacga gcgcgacctc gacgaccctg gcgccgcgct cacgatcatc cgtgcccacc
     2041 atcgcgaggt cgactcgacg gtgttgcacc ccgcggacgt gtcgtggttc gtcgaactgt
     2101 gtcggcgtcc gggcaagccg gtgcccttcg tgccgatcat cgacgccgag gtccggcgct
     2161 ggtggcgatc cgattcactg tggcaggccc acgacgaccg ctacgacgcc gacggcgtca
     2221 tcgtcatccc cgggccggcg gcgcttcgag cggtgagagc ggtcgacgag ccggttgcgg
     2281 cactgttcaa ccgcttcgag gcggcgacgg tcgccgcgct ggtcgaggcg ggggtcgagc
     2341 cggtcgacgc cgcccaacgg cgcgagaccc acaccgacca ccgcgaccga cgcggcgatg
     2401 atcccatctc tcggctgctc gcagaaccca acgtggaatg gaccaccgct ggccccgacg
     2461 gactccggcg ccgccgacgg ccgaacccgg cgctggcggt cgccccggcc acggcgtggc
     2521 atctcgacgt gtcgggcaac gggcacggac ccgcttcggt cgcgcgcgcc caccgaagcg
     2581 atgccgtcct ggtcgccacc ggcccgaact cggtgcgcct cgacgttccg ttgacggttc
     2641 accgcgacac gatgtccacc gcgtcgatcg agttcgacct cagcagcgac ggccggtcgg
     2701 cggccgtggt gtcaccccgg agctcagccg catggtccgt ggccctgcgc tgcgcatctg
     2761 tgcctgtgca tggccccgat ccgacccccg atcagacgag cgcgacgggc accgagtttc
     2821 tgctcgccac cgcgctcgat catgccgcgc tgttcggcgc agcctcgccg ctgcccgatg
     2881 ccctcgtcgg tccctgctgg tccgcggtct tcgctgcgct cgagcgcgag ttcgacgtgt
     2941 cgtcgggcgg gttcgccgac ctgttgcacc tcgaccacca cctcgagttg cacggcggct
     3001 ccatgccgac ccgccctgcg ttgcccgaca ccgacggcaa cctcgacatc gacgttcgcg
     3061 tcgagtcgaa caccgccacg tcggccggac gcgccgtcgc ggtgcgcatc gaactcgcca
     3121 acgcccacgc aaaggtcgca acacttcacg agcgtttcct gatccggagc gacatcggta
     3181 aggaaccgct gggcgccccg agggcgttga ggctggccga cgacgccgcc ccgacccctc
     3241 ggcgcacgct ggccgacatc gaggtgaaca ccccgccgaa cggtatcgcc atggcgagct
     3301 tcaccggtga tcacaatccg ctccatctga gccgcgaggt cgccgcggtg gcaggcttca
     3361 ccgcgccgat cgttcacggc atgtgggtga gcgcggcgat gcaacaggcg gcgcgccgct
     3421 cggcagcggg tacgcacggt ggcacaggcg tcgaggtgcg cgaatggcac gcgaacttcg
     3481 tcgctccgat cccgcacgat tcgccggtat cgatctcggt cgtgcgggtc gcacgcatcg
     3541 atgggggcga tgtggtcgag gtgcaggcga ccgtcgacgg ggacctggtg gcgaccgccg
     3601 ccggggtcct cagcgcgccg cgcaccgtct atgcctttcc cggccagggc attcagcgcg
     3661 tcgccatggg cctcgatgcg ctcacgcggt ccgcagcggc gcgcgccgtg tgggaacggg
     3721 ccgatcatca cacccggtcg gccctcggct tttcgattcg gcacgtcgtg cgcgacaacc
     3781 ccgtcgaact cgtcgtcgac ggcgaacgat tccgtcaccc cgacggcgtg ttgttcctca
     3841 cccagttcac ccaggtcgcg atggcgaccc tggcgagcgc ccagatcgcg gagctgcgcg
     3901 aggccggcgg tttcgtcccc gatgcgatcg cgtgcgggca ctcggtcggc gaatacaacg
     3961 cgctggcggc ggtgaccggg atcctggcgc tcgaagacgt cctcgaggtc gtctaccagc
     4021 gcggcctcgc catgcaccat ctggtcccac gcgatgcggc cggcaactcg cgctatcgca
     4081 tggcggcggt gcgcccgtcc gaagccggcc tctccgaatc ggagctccgc gagttggtcg
     4141 ccgacctcgc tcgcgacacc gaccccgagg agttcatcca gatcgtgaac ttcaacttgc
     4201 gcggccgcca gtatgcggtg gcgggaacgc aggcaccgct acgacgtctc cacgacgaac
     4261 tcgaacgccg ccgggccgaa cgcaacggca aaggcgtcta cgtcgaggtg cccggcatcg
     4321 acgtgccgtt ccactcggcg gtgttgcacc acggcgtcgc cgggtttcga accaaactcg
     4381 acgcgatcct gcccgaccgg atcgacccga ccctgctcgt cgggcgctac atcccgaacc
     4441 tggtcccacg gttgttttcg ctgtgtcgcg acttcgtcgt cgaggtcgcc gaggtcgcgg
     4501 aggccccggc gttgaactcg gtcatcgaac gctgggatga ctggtcgcag cgccccgaag
     4561 cactgtgccg ggtgctgttg ttggaactgt tggcctggca gttcgccagc cccgtgcgtt
     4621 ggatcgaaac ccaggacctg gtgttcgcac tcgacagcgg catcgagcgc ttcgtcgagg
     4681 tcggcctcga aagccaaccg acgatttcct cactcgcccg ccagaccctg tcgggtgagc
     4741 ccggacggta tgcagcggtg acggtggcga acctcgaggc cgaccgcgcc gagttgttcg
     4801 gggaggacac cgacccgcca ttcgacgaac tcgcggaagc cgccgacgat ggccgggagc
     4861 gggcgggcga ggcgggcgag gcgggcgccg aggaacctcc ctccccctcg gcgcccaccg
     4921 ctcccgctgg tgccaccagt gccgccagtg ccgctggtgc cgccgtggcg ccggaggacc
     4981 tcgcgtggac gccggccgac ggtcttcgct tcatgttgtc gtggtggacc aaggtccgca
     5041 tcgaccaact cgccgacgac gacaccatcg agtcgctggt cgacggcgcg tcttcgcgcc
     5101 gaaaccaact cctgatggac atcggcaagg aattcggcct cggcgcgatc gacggggcgg
     5161 ccgacgccgc gctgtcgacg ctgatcgatg aggtcgccgc ccgggcgcgc acttaccgcg
     5221 cgcccgggcc ggtactgcgc cccacgatcg acgaacagct ccggcgggtc accggcccgg
     5281 cgcggtcgcg tccacgagcg atcgacgaac gaatcgccaa ccactggggg ctgggtgccg
     5341 gctggacgac ggtcgttgcg gcagctgtcg ctgcgggcac ccgtgcaggc accagcgtgc
     5401 gcgggggcga cctggcgacg ctgagcccca ccgagccggc ctcggccgag gatctcgacg
     5461 gcatcctcga cgccgcggtc accgcggtgg cccgggccag cggtgtcgag gtcgcgccgt
     5521 tggtcgctcc cgggggttcc gacgcgctcg acgcagcgac ggcgaccgcg ttgctcgaca
     5581 cgatcatcgg tgccgacgga gtgctcaccg agatcggccg ggtcatcacg cgccgtacga
     5641 gcgacgcacc gaccggcgat tcgagcttcg accttcagga gacccagcgc ctccacgaca
     5701 tcgccgcggc ggtgacccgg gaactcggcg ccggctggca agcagccgtg gccccggcct
     5761 tcgatccggc caggaccctg gtcatcgacg accgctgggc cggccaacgc gaggacctcg
     5821 cccgcttggc caacagcgat cccgatcccg atcgggatcg caccgaccgc gagcggcggt
     5881 tcgcccgcct cggccgcggc ctcgcgccat ccgcgatcgg gcacgcccgc tggcacgaac
     5941 gcgaggcccg acgccgtgga gacgtcgacc tggccgatcg cctcgcccgc ctcgtcgccg
     6001 agtcggccgc gaccgcggtt cgaaccgatg acctgagcga cgacgtggcg ttggtgaccg
     6061 gggccagccc ggggtcgatc gcggcgggga tcgtcgccga gttgctcggc cgcggcgcca
     6121 ccgtgattgc gacggcatcc tcgctcacca gcgagcgaat cgcgttcttc cgcgagctgt
     6181 accacgacca cgcccaccac cgggcctcgc tgtggttggc gccggccaac ctggcatcgt
     6241 tcagcgatgt ggaccgcctc gtcgaatggc tcggcaccga cgtcgacgag gccaccggag
     6301 gcgagccgag aatcgtcaaa ccagcgctga cgccgacgtt ggtgttcccg ttcgcagcac
     6361 cggcggtacg cggcgacctg agcgaggtcg gcccgtccgc ggaactcgag atgcgggtgt
     6421 tgttgtggtc ggtcgaacgg ctcatcgcag cactggccga gcccgactcc ggccgggatc
     6481 cggcgacgcg tctgcacgtc gtgctgcccg gatcacccaa tcgcggcacc ttcggcggtg
     6541 acggcgccta cggcgaggcg aaggccgcac tcgatgcgct gaccacgcgc caggcccatg
     6601 aaccatgggg cgagcgcgtc tcgatcgtgc acgccatcat cggctgggtt cgcggcaccg
     6661 ggttgatgtc gggcaacgac gccctcgtgc cactcgtcga gaccgaaggg atcggaacct
     6721 ggagcgccga ggagatcgca cgggaactcg tcgacctcgg tgaccgcgac caccgccacg
     6781 cgtccggcgg agccctcacg gtggatctgt cacgggggct gtggaattcg acgatcgacc
     6841 ttgccgacct tgccgcacag gcccgcgacc tggcgtccgc agccgatata gcggccgtag
     6901 agcctgcagc gcctgactcc cccgtgctca tcgacgccct tccccacccg ccgagcgccc
     6961 cggcgcccac cggaatcgac tggccggcac tggatctcac cccggccgac accgtcgtga
     7021 tcgtcgggtt cggcgaggtc ggcccgctcg gctccgccag gacccgtttc gacgtcgagg
     7081 tcaccgactc gctgtccgcc ggcgccgtcg cagagctcgc ctggatgtgc gggctcatcc
     7141 gctgggagtc caccccgacc cccgggttct acgacgtcga gtcgggcgaa ctcgtcgcgg
     7201 agcatctcat cgccgagcgc tacgaacagc ggctgctcga caacagcggg gtgcgcttcc
     7261 tcgacgacga cggcgcccag gtcgacggca cggctcccct gctggcctcg gtgtttctcg
     7321 aacgcgatct cagcttcgtc gtcgagtccg aggcgcaggc cgaagcgttc gtcgccgccg
     7381 atccggaccg cacgagtgcc acaccgaacg ccgacggcac cttcacggtc acccgcagcg
     7441 ccggcaccga aatccgggtg ccgcgcagaa ccgtgcttcc tcgacgggtc gccgcacaac
     7501 tccccgcggg gttcgaccca tcggtgtacg gaattccgtc cgacatgatc gaggcgctcg
     7561 atcgcgtcgc ggtctggagc ctcgtggcga cggtcgacgc gttcgtcgac gccggaatcg
     7621 aaccggccga gctgttgagc tgggttcacc ccaccgaagt cgccaacacc caaggcaccg
     7681 gcatcggcgg catcagcgcc atccgacgca tgtacgtcga ctcgctcctc ggcgacgagg
     7741 cacccaacga cattctccag gaggcgctcc ccaacgtcgt ggccgcgcac gtcatgcagt
     7801 cctacgtcgg cggctacggg gccacgatcc acccggtcgc cgcgtgtgcg accgccgcgg
     7861 tgtcgctcga ggaggcgttc gacaagattc gcagcggtcg ggccgccgtc gtcgtcgccg
     7921 gcggattcga cgacttcggc cacgaggggc tcatcggctt tgcggccatg aacgcaacgg
     7981 cgtcgagcga cgcgatggag gccgccggga tcgaggcgcg ccggatgtcg cgggcgaatg
     8041 accggcgccg cgccgggttc gtggagggac agggtggcgg caccgtcgtg gtggcccgcg
     8101 gcgacatcgc ttacgaactc gggctccccg tgcgcggtgt catcgcgtgg gccggttcgt
     8161 tctccgacgg catcaacacc tcgatcccgg cgccaggcct cggcctgatc ggcgcggtcc
     8221 gcggcggtgg ggcatccccc ctcgcccgtg cgctgaggtc gttgggcctc gacgccgacg
     8281 acatcgccgt ggtgtcgaaa cacgacacgt cgaccgccgc caacgatccg aacgagtcgc
     8341 tgattcacga acggctgtcg agcgctctgg gccgacacga ggacaacccg ctgtacgtca
     8401 tcagccaaaa gacggtgacc ggccacgcca agggcggcgc cgcggccttc cagaccaacg
     8461 gactctgcca ggtgctggaa tcgggggcaa tcccgccgaa ccgcagcctc gattgcgtcg
     8521 acggcgacct cgaacggtac gcccgactcg tgtggccgac cgagccgctg catcaccgtg
     8581 gctctctgcg ggccggcctt ctcaccagcc tcgggttcgg ccacgtcgcg gccatcgtcg
     8641 ccatcgtcca ccccgaggcc ttcgtcgcca ccatcgaacc cgaccgacgc gacgactacc
     8701 ggcggcgagc cgcccaacgt agcgaacgcg gccagcggcg gcgggtggcc gcccgggtcg
     8761 gcggagcgcc gctgtacgtg cgtcccgacc accgtctcgg cgaccgtccc gccgcccgcc
     8821 gcgacctcga ggcggcgatg ctggtcgacc ccgacgcacg cctcggcccc gacggcacct
     8881 accgggtccc cacgatcgac caggcggcgt catgatcgcc ggggtcgggg tggatgtcgt
     8941 cgcggtgagc gggtttcgcg accaactcga actcgacgcg acgacgttca ccgaggtctt
     9001 cacccccgcc gagcgcgacc ttcgggcgac gggccgcggt gatgccgagc acctcgcggc
     9061 gcgatgggcg gccaaggagg cgttcgtgaa ggcgtgggca gggtcccgtc atgggcgtcc
     9121 gccgcaactg cagtcggtgg agatgcgcga catcgaggtg gtgtgcgacg catggggtcg
     9181 gccggcgctc aggctgcatg gcgaggtcga cgaagagttc tcgcgttcgg ttccggacgc
     9241 ctcggttcac ctgtcgatct cccacgacgg cgacgtggcg atcgcgtacg tcgtcatcga
     9301 tcgggtgatg ccatgacccc gacgaacacg gtcgaaacct ccgacatcaa cctcaaagcg
     9361 ccgatggcct gtctcgtggt ggccatgctc gtcgacgt
//
LOCUS       DAOOMA010000027         8909 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_9172_length_8909_cov_4.348656, whole genome shotgun sequence.
ACCESSION   DAOOMA010000027 DAOOMA010000000
VERSION     DAOOMA010000027.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 8909)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8909)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8909
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9172_length_8909_cov_4.348656"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..244
                     /locus_tag="PLP95_01570"
     CDS             <1..244
                     /locus_tag="PLP95_01570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB44519.1"
                     /translation="WNDSTSTETVILPKAANFEGVPGWYIGHLPDGALATYEDVSVGY
                     DTAWRRQSTTSTTAPGPTLPVPALSNPLEPFGGRPA"
     gene            complement(489..833)
                     /locus_tag="PLP95_01575"
     CDS             complement(489..833)
                     /locus_tag="PLP95_01575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB44520.1"
                     /translation="MATPVLEKITTVQTQVVDTIALVKEPITTGVTTVVDFVIGKVPN
                     IPAVPYADQIPTPKEVIDNQYKFAKSVIDVNKDIALSLAKAAAPITDKVLDRHTPAAR
                     KGTTATAAKKSA"
     gene            1073..1708
                     /locus_tag="PLP95_01580"
     CDS             1073..1708
                     /locus_tag="PLP95_01580"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015798293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HPB44521.1"
                     /translation="MTVRLGDEAPDFTAETTEGTINFREWKGDSWAILFSHPKDFTPV
                     CTTELGTVARLKPEFDKRNVKAIGLSVDELTNHHGWADDIEETQGTRPNYPIIADTDR
                     KVSDLYDMIHPNADNTLTVRSVYIIGGDNKVKLILTYPASTGRNFDEILRVIDSLQLT
                     SKHSVATPADWKQGDEVIIGSTVSDDDAKEKFPEGWRAEKPYLRYVAQPQG"
     gene            complement(1758..5291)
                     /locus_tag="PLP95_01585"
     CDS             complement(1758..5291)
                     /locus_tag="PLP95_01585"
                     /inference="COORDINATES: protein motif:HMM:NF024589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HPB44522.1"
                     /translation="MKCGTCGSENFHDARFCAFCGGPLRVPADERRVVTVVFADLVGF
                     TTLSEHLDPERLKGIVDHAFERLVQDIHDFGGRVDKIIGDAVVALFGAPVAHEDDAER
                     AVRAALRMHETLAAYSAEVEVDIRMRIGVNTGEVLVGALRAGGDYTAMGDVVNTASRL
                     ETSANPGEVLVGPATHAATLAVIGYESRGSLIIRGREGAVDVWAAKEALVPPGHRSRR
                     HIPIVGRDAEVTLLRNLGELSMRNRRAQSLVIIGEAGVGKTRLVEELAPIMLGIEPTT
                     VMFSGRCVPYGESNPWWPIAEVLRDACSVELSDSVDVARSKCTAAVREVMEPDAVADV
                     TARLLLLLGYDDPSRSRDPDRLAESTRQRHDPASGFLAFLEATLRVRPVLMRLADLHW
                     AEQVVIELVDEMMVRMARRPLVFVATGRRALTERWSPATGRHNTLVLNLGALPRQASE
                     QLLDSILGAGSPQAVRDMLIDRSGGNPLYLEELATLIGGREHASATLDLDELPDTLRG
                     LIGARIDDLTADEQGVLADASVWGPEGPVAALRELGQTIRGRADVDPLIATLSLKDIL
                     VAESGVWRFRSDLLREVAYSRLTKSERMRRHRGIAEYIGERRPGVPDTAAVDLLARHY
                     GEAARLTLELDPTAPDRELIAAAIQWIGEAARRAADGDSWVAAATLNEQGIELAAAVD
                     RAADLLRFTTGRGTARCELWDNDGAQHDAAAALDLADRLGDEAGRAAAELLRGAVDAR
                     SNRVEEAHVWFDRAIERFRDIGDERGRAVALRRKGMASLVVGNAREAEGPLNESLVAF
                     RQLGDRRGQAWALQNLAWIGFITGRTAVAEDRLADAYQVFAELGDPTGTAWCDGLLAY
                     VRYYQGRFAEANTLARDSFDAAKRRGDRWGQAMSLVVVGATDLWEGQSAASAKRTADA
                     LKLFEQMHDMSWVRQTTALHARALAASGRVTESLAALERLDSGSEHSGWIGQPLMVTA
                     GVLATLVHLGGGSTDGDRLFDDILSAVEGAENPAPVELYNAAILASLQRGSLDRAIEF
                     AALCEREPGESGQAPSVVRNGYALSAPALLAAVRGEHDAVAALLASDEPVTYTDRALL
                     HVAVALGRGEGWLGNLAFAESVVRATDDVVSQATVALAKATIGRAVGHLDAPRWEALA
                     AEHWDAIGIEPSGWTRVFLTAVATMPAAPMS"
     gene            5449..6996
                     /locus_tag="PLP95_01590"
     CDS             5449..6996
                     /locus_tag="PLP95_01590"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HPB44523.1"
                     /translation="MVWWRRPALWAAMSAIAVLIGNLRGIAVGDDGVGYQAIADSLLA
                     GEGLGYFLERPLTIWPPGWPALMAAVAKVTPLDTVQAAVLLNMITAAVVVLLLHAILS
                     RLTRHELPRFAGMAVTAVGASSMIFGHLLMTDFAFAAITLGLFLVMFRYRDTGSFVWL
                     AAAAALVWAGFLTRYAGVVHIGTVGLWVLADRRTALSKRVVHGIGFGVAAAVLPAAWV
                     ARNIDADGTTLGERYSSARGLIHNIYDTAATIGNFLSPAIESERRGLWILVGAAGSAV
                     VVAMLWRAVRADTRFRSARGLLDVVASPVGLVALHVAVYAAYMLYARTTTGLNQLDFR
                     LLNPIYLPLVICALVVADRLLALPDTPENSLWSRAARYCVVAWAVLNVVVGIGMVGFF
                     TTDPALFPGNYERDEFEVVRESPALDAIPADCPTVSNLPNALYGSGVDARWSWRHTGL
                     ESNDVVDDLDQLIAGTEAGTRYCLVWISLDPTYGHLVTLDELGDLVTLRPLASGTHVD
                     VYEVTAR"
     gene            7299..7721
                     /locus_tag="PLP95_01595"
     CDS             7299..7721
                     /locus_tag="PLP95_01595"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HPB44524.1"
                     /translation="MSRFDYLEPLRNIRLFHGCSDKELEQVARLSDEVDIAEGHRIVE
                     QGDSAREAFVILEGTATVVLDNRAIAELSVGDHFGELALLDGGTRTANVDAATPMRLL
                     VIHRPAFMGLLDEQPGIARKIMATLAGIIRDLEHPPGG"
     gene            7835..>8909
                     /locus_tag="PLP95_01600"
     CDS             7835..>8909
                     /locus_tag="PLP95_01600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HPB44525.1"
                     /translation="MTASGTTDTAAEKRFRPRHIALIIGFGFAAITALSFAAEKLWAE
                     EWQHQYLEEKSQTREFFFNVPKAFVIGFYLITIISIVYGAISFSNRMRNWERGAPESR
                     KTTTKNVKKRAEKVRAGLYMQTLLRDPAAGIMHSLIYFGFLILLAITTIGEINLQAPV
                     GLKFLHGGTYKAYAFVADLAGLAFTVGVIWAIIRRYVQRPYRIRIKSKPEHALILATM
                     LAIGVTGFGAEVFRIVSQGMPNYEKWAFVGYPIATLFEDVSWAHGAHQWFWAAHIFSF
                     IAFVIMLPSTMLRHIFTSPINMYLSERDRPKGAMKAMPNLMETELENFGASVVEDFTW
                     KQLLDTDSCTMCGRCTSVCPAHAT"
BASE COUNT         1575 a         3061 c         2713 g         1560 t
ORIGIN      
        1 ctggaacgat tcgacatcga ccgagaccgt cattctgccg aaggccgcga acttcgaagg
       61 tgtccccggg tggtacatcg gccacctacc cgacggcgcg ctcgcaacct atgaggacgt
      121 gagtgtcggt tacgacactg cgtggcgccg acagagcacc acctcaacga cggcacccgg
      181 cccgaccttg cccgttcccg ccttgtccaa cccgctggag ccgttcggcg gcaggccggc
      241 ctgataccgc tggccgaacc ggacgagttc gcaccggccg agttcgaaaa cgcggcgaag
      301 gccccgtggg gggacggggc cttcgcactt tcgcatgtcg ccgtggggtc ccggcacgaa
      361 aaggggggag gattcgtggg gccgcggcga ttgcaagcgc cgacaggggg gtgtcagcgt
      421 cgtcccggag ccatcagggg ggatgtggct ccgggcccgt tcacccgtag ggaacggtga
      481 ctttggacct acgccgactt cttggcggcg gtagcggtcg tgcccttgcg agcggccggg
      541 gtgtgacgat cgagcacctt gtcggtgatc ggcgccgcag ccttggccag cgagagggcg
      601 atgtccttgt tcacgtcgat gaccgacttg gcgaacttgt actggttgtc gatgacttcc
      661 ttcggcgtcg gaatctggtc ggcgtagggc acggccggaa tgttcgggac cttgccgatg
      721 acgaagtcga cgacggtggt cacgccggtg gtgatgggtt ccttcaccag agcgatcgtg
      781 tcgacgacct gggtctggac ggtggtgatc ttctcaagga ctggagtagc catgatgttt
      841 cctttcggcc gagggagccg ctggggggag tgcgactcaa ggcttgatag tgctaactgt
      901 agcaagcaac cgcttcgact gcaagcaaat gcttgcaatt gtttgcagcg gcatccgtca
      961 caccgccttg ccctgtcgat cttttgaccg gtccagctaa acccgactag tttggtcggg
     1021 aattgggaat catccgatcc ccacacaaac gaagcaacaa gtgaggcatt acatgaccgt
     1081 tcgacttggc gacgaggccc cggatttcac cgccgaaacc acagaaggca ccatcaactt
     1141 ccgcgagtgg aagggtgaca gctgggccat cttgttctcc cacccgaagg acttcacacc
     1201 ggtttgcacc accgaactcg gcaccgtcgc ccggctcaag ccggaattcg acaagcgcaa
     1261 cgtgaaggcg atcgggctct cggtcgacga gctcaccaac caccacggtt gggccgacga
     1321 catcgaggaa acccagggca cccgtccgaa ttacccgatc atcgccgaca ccgaccgcaa
     1381 ggtgtccgac ctgtacgaca tgatccaccc caacgccgac aacaccttga ccgtccggtc
     1441 ggtctacatc atcggtggcg acaacaaggt gaagctcatt ctcacctacc ccgcatcaac
     1501 tgggcgcaac ttcgacgaga tcctgcgggt gatcgactcc ctgcaactca cgtcgaagca
     1561 ctcggtcgcc accccggcgg actggaaaca aggcgacgag gtcatcattg gctccacggt
     1621 cagcgatgac gacgccaagg agaagttccc cgaaggctgg cgcgcggaga agccctatct
     1681 tcgctacgtc gcccagcccc agggctgagg ttcgcacctc accagcgatt cacccgatgc
     1741 ccgtcgtctt cgtgtcatta cgacatgggg gcggcgggca ttgtcgcgac cgcggtgaga
     1801 aacacccgcg tccaacccga cggctcgatc ccgatggcgt cccagtgctc ggcggccaac
     1861 gcctcccaac gaggcgcatc gagatggccg accgcacggc cgatcgtggc cttcgccaac
     1921 gcgacggtcg cctgagacac gacgtcgtcg gtagcgcgca caaccgactc agcgaacgcg
     1981 agattgccga gccaaccctc gccgcgaccc agcgccaccg ccacatgcaa caaggcgcga
     2041 tcggtatagg tcaccggttc gtccgaagcc aacagcgccg caacggcgtc gtgttcgcca
     2101 cggactgccg ccagcaacgc cggcgcactc agtgcatatc cgttacgaac gacgctcggc
     2161 gcctgacctg attcgcctgg ctcgcgttca cacagcgccg caaactcgat cgctctgtcc
     2221 aacgatcctc gctgcaacga ggcaaggatc gcggcgttgt acaactcgac cggcgctggg
     2281 ttctcggcac cttcaacagc cgaaagaatg tcgtcgaaga gtcggtcgcc atctgtcgag
     2341 cctccgccga gatgcaccag tgttgcgagc acaccggcgg tgaccatcag cggctgaccg
     2401 atccaccccg aatgctcgct cccgctatcg aggcgctcga gggcagcaag gctttcggtc
     2461 acccgaccgc tcgcagcgag tgcccgggcg tgcagcgccg tggtctgacg gacccacgac
     2521 atgtcgtgca tctgttcgaa cagtttcaac gcgtcggcgg tgcgcttggc cgacgcagcg
     2581 gactggcctt cccacagatc ggtcgcgccg acgacgacga gcgacatcgc ctgaccccag
     2641 cgatctcccc gccgtttcgc cgcgtcgaac gagtcgcgag cgagcgtgtt cgcctcggca
     2701 aagcgcccct ggtaatagcg aacgtacgcc aacaatccgt cgcaccatgc ggtccccgtc
     2761 gggtctccaa gttcagcaaa cacctgatag gcgtcggcga gccggtcttc cgccaccgcc
     2821 gttcgaccgg tgatgaatcc gatccacgcc agattctgca gcgcccatgc ctgcccccga
     2881 cgatcgccaa gctggcgaaa cgcaaccagc gactcgttga gcggcccttc agcctcgcgg
     2941 gcgttaccga caacgagcga ggccatccct tttcggcgaa gggcgacggc ccgtccgcgc
     3001 tcgtcgccga tgtcacggaa ccgttcgatg gcccggtcga accaaacgtg tgcctcctcg
     3061 acgcggttgc tgcgcgcatc gaccgctccg cgaagcaact ccgcagcggc ccgtccggcc
     3121 tcatctccga gacggtcggc cagatcgagt gccgcggcgg cgtcgtgttg cgcgccatcg
     3181 ttgtcccaca attcgcaccg agcggtgcct cgccccgtcg tgaacctcag taggtcggca
     3241 gcgcgatcga ccgccgccgc cagttcgatc ccctgctcgt tcagcgtcgc cgccgcaacc
     3301 caggagtcac cgtcggccgc tcgccgagcc gcctcgccga tccactggat cgccgccgcg
     3361 atgagttcgc gatccggcgc agtcgggtcg agttcgaggg tgaggcgcgc cgcctcaccg
     3421 tagtggcggg ccaacaggtc gaccgcagcg gtgtcgggga ccccggggcg acgctcgccg
     3481 atgtactcgg caatgccgcg atgtcgacgc atacgttcgc tctttgtcag ccgcgagtac
     3541 gcgacctcac gcaggagatc gctgcggaac ctccacacac ccgactcggc gaccaggatg
     3601 tccttcaacg acagggtggc gatcaacggg tcgacgtcgg cgcgaccccg aatcgtttgc
     3661 ccgagttctc ggagcgcagc caccgggcct tccggccccc ataccgaggc gtcggccaac
     3721 acgccctgct catcagcggt gaggtcgtcg attctcgccc cgatgagccc ccgcaaagtg
     3781 tcggggagtt cgtcgagatc gagtgtggcc gaagcgtgct ctcgtccgcc gatgagcgtc
     3841 gccaactcct cgagatagag aggattcccg ccgctgcggt cgatcagcat gtcgcgcacg
     3901 gcctggggcg agccggcacc cagtatcgag tcgagcaact gttcggaggc ctgcctcggc
     3961 agggcaccca gattgagaac cagtgtgttg tgacgtcccg tcgccggcga ccaacgctcg
     4021 gtcagcgccc gtcgtccggt cgcgacgaac acgagtgggc gacgagccat ccgcaccatc
     4081 atctcgtcga ccaactcgat caccacctgt tcggcccagt gaaggtcggc caaccgcatg
     4141 agcactggtc gaacacgcaa cgtcgcttcg aggaaggcca aaaagcccga tgcgggatcg
     4201 tggcgttgcc gggtcgactc cgccagtcga tcggggtcgc gcgatcgaga cgggtcgtcg
     4261 tatcccagca ggagcaacag gcgcgccgtc acgtctgcga ccgcatcggg ctccatcacc
     4321 tctcggaccg cggcggtgca cttggaacgg gcgacgtcga ccgaatcgga caattcgacc
     4381 gagcacgcgt cgcgcagaac ctccgcgatc ggccaccacg ggttcgactc accgtaggga
     4441 acgcagcgac cgctgaacat caccgtcgtc ggctcgattc ccagcatgat cggcgccaat
     4501 tcttcgacaa ggcgggtctt tccgacgcct gcctccccga tgatcaccaa cgactgggct
     4561 cgccggttgc gcatggacaa ctcgccgagg ttgcgcaaca gcgtcacttc cgcgtcccgg
     4621 ccgacgatcg gaatgtgtcg ccgcgaccgg tgcccgggag gaacgagcgc ctcctttgcc
     4681 gcccacacgt cgaccgcacc ctcacgccca cggatgatca acgacccgcg ggactcgtag
     4741 ccgatcaccg caagtgtcgc cgcgtgggtc gccgggccga ccagcacctc gcccgggttg
     4801 gccgaggtct cgagtcgcga tgcggtgttc accacgtcac ccatcgcggt gtagtcaccg
     4861 ccggctcgca gcgcgccgac cagcacctcg ccggtgttca caccgatgcg catccgtatg
     4921 tcaacctcca cctcagcgga gtaggcagcg agcgtctcgt gcatgcgaag tgcggcgcgc
     4981 accgcccgtt cggcgtcgtc ctcgtgtgca accggagcgc cgaacagcgc cacgacggcg
     5041 tcgccgatga tcttgtcgac ccgaccaccg aaatcgtgga tgtcttgcac caggcgttca
     5101 aacgcgtgat cgacgatgcc cttcagccgt tccgggtcga ggtgttccga cagggtcgtg
     5161 aacccgacca ggtcggcaaa tacgacggtg acgacgcgac gttcgtcggc cgggacccgc
     5221 aacggtccac cgcagaatgc gcagaaccga gcgtcatgga aattctcgga tccgcaggtc
     5281 ccacacttca caggaccatc ctacgaacga acccgtgccg gtcggtacac ttgtccccca
     5341 cgacacatgg cccccgctcc gcgtggccgt tcgggcaagc agcgccgcaa ccgcggcacg
     5401 gctcgcggcg cctcgcgagc ccagcgacac cgaggagagg attcgatcgt ggtgtggtgg
     5461 cgacgtccag cactgtgggc tgcaatgagt gcgatcgccg tactcatcgg caacctccga
     5521 ggtatcgccg tcggcgacga cggggtcggg tatcaggcga tcgccgattc gctcctcgcc
     5581 ggcgagggcc tcggctactt ccttgagcgc ccgctcacca tctggcctcc cggatggccg
     5641 gcgctcatgg cggcggtcgc aaaggtgacg cccctcgaca ccgttcaagc tgcggtgctg
     5701 ttgaacatga tcaccgcggc ggtcgtggtg ctgttgctgc atgcaatcct cagccggctc
     5761 acacgtcacg agttgcctcg cttcgccggc atggccgtca ccgccgtcgg cgcgtcgtcg
     5821 atgatcttcg gtcatctcct catgaccgac ttcgcgttcg ccgcgatcac gctgggactg
     5881 ttcctggtga tgtttcgcta tcgcgacacc gggtcattcg tgtggctcgc cgcggctgca
     5941 gccctggtgt gggccggttt cctgacccgc tacgccggag tcgtacatat cggcacggtg
     6001 ggcctctggg tcctggctga tcgccgtaca gcattgtcga agcgggtcgt ccatggcatc
     6061 gggttcggtg tggccgcggc ggtgttgccc gctgcgtggg ttgcccgaaa catcgacgcc
     6121 gacggcacca cgctcggcga gcggtactcc agcgcccgag gcctcatcca caacatctat
     6181 gacaccgcgg cgacgatcgg gaacttcctg tccccagcga tcgaaagcga gcggcgcgga
     6241 ctctggatcc tcgttggcgc ggccggatct gcggtcgtcg tggcgatgct gtggcgtgcg
     6301 gttcgcgccg acacgcgctt tcgttcagca cgcggcctcc tcgatgtcgt cgcctctccg
     6361 gtgggactgg tcgccctcca cgttgccgta tacgccgcgt acatgctcta tgcgcgcacg
     6421 acgaccgggc tcaatcaact cgactttcgt ctactgaatc cgatctatct cccactcgtc
     6481 atctgcgccc tggtcgtcgc cgatcgcttg ctggcgttgc cggacacgcc cgagaactcc
     6541 ctgtggagcc gcgcggcgcg gtactgcgtt gttgcttggg cggtgctcaa tgtcgtcgtc
     6601 ggcatcggca tggtcggctt cttcacgacc gacccggcgc tgttccccgg caactacgag
     6661 cgcgacgagt tcgaggtggt tcgcgagtcg ccggcactcg acgcgattcc cgctgactgt
     6721 ccgacggtgt cgaacctccc caatgctctg tacggctccg gggtcgacgc cagatggtcg
     6781 tggcgacata cgggcctcga gtcgaacgac gtcgtcgacg acctcgatca actgatcgcc
     6841 ggaaccgagg ccggaacccg ctactgcctg gtatggatct ccctcgaccc gacctacggt
     6901 cacctcgtga ccctcgacga actcggcgat ctcgtgacgc ttcgaccgct cgcgtccgga
     6961 acgcacgtgg acgtctacga agtcaccgct cgctgagttc gaacgcagag atagaaactg
     7021 ccacgtccgt cggcccggct tgcgagcgaa cccggtgagt gttccggaga cgtcgtgggt
     7081 ctcaacagat caatcatccg aaggttcgcg ggaggtgtcg acgaacgtgg cagtttccga
     7141 tcatcagcgc ttcaacccaa cccccttttc ggcttttcga atcgctgcgc tgacctggaa
     7201 ctcagtatgc ctacgcccca cgatcctgtc aacccaagag atggaaacta tctgcatttt
     7261 agatagctcg ccgagaccca tgagctaagc tgacgggcgt gtcacgtttc gattacctcg
     7321 aaccgcttcg gaacatccga cttttccacg ggtgttccga caaggaactc gaacaggtcg
     7381 cccgcctcag cgacgaggtc gacatcgccg agggtcaccg catcgtcgaa cagggcgatt
     7441 cggcccggga ggcgttcgtg atcctggagg gaaccgccac ggtggtgctc gacaaccgag
     7501 ccatcgccga actctcggtc ggtgaccact tcggcgagct cgcgctcctc gacggcggca
     7561 cccgaacggc caacgtcgat gcagccaccc cgatgcgtct gctggtgatc catcgacctg
     7621 cgttcatggg cctgctcgac gaacagccgg gcattgctcg caagatcatg gcgacgctcg
     7681 ccggcatcat cagagatctc gaacacccgc cggggggctg acccgcgccc gtcgccggcc
     7741 ccccggatca cggagccgcg gttaggcggc cctatcacat tgtcgaggcc gatcgttttc
     7801 ggttactgag gttccaccga gtaccttggc cggtatgact gcgtctggaa ccaccgacac
     7861 cgcagccgag aagcgcttcc ggccccgcca catcgcgctc atcatcggct tcggcttcgc
     7921 tgcgatcacc gcgttgagct tcgcggccga aaagctgtgg gccgaggagt ggcaacacca
     7981 atacctcgag gagaagagcc agacccgcga gttcttcttc aacgtcccca aggcgttcgt
     8041 catcggcttc tacctcatca cgatcatctc gatcgtgtac ggcgccatca gtttctcgaa
     8101 ccggatgcgc aactgggagc gcggcgcacc cgagtctcgc aagacgacca ccaagaacgt
     8161 caagaagcgc gccgagaagg tacgcgccgg gctctacatg cagacgctgc tgcgcgaccc
     8221 cgcggccggc atcatgcact cgctgatcta tttcggcttc ctcatcttgt tggccattac
     8281 cacgatcggc gagatcaacc tgcaggcgcc cgtcgggctg aagttcctgc acggcggcac
     8341 ctacaaggcg tacgcgttcg tcgccgatct ggcgggcctc gccttcaccg tcggtgtcat
     8401 ctgggcgatc atccgccgct acgtgcagcg cccctaccgc attcggatca agtcgaagcc
     8461 cgagcacgcg ctcatcctcg cgacgatgct cgccatcggc gtgaccggtt tcggcgccga
     8521 ggtcttccgc atcgtctccc agggcatgcc caactacgag aagtgggcct tcgtcggcta
     8581 tccgatcgcc acgctcttcg aggacgtgtc gtgggcgcac ggggcccatc agtggttctg
     8641 ggccgcgcac atcttcagct tcatcgcctt cgtcatcatg ttgccctcga cgatgctgcg
     8701 ccacatcttc acctcgccga tcaacatgta tctcagcgag cgcgaccgtc ccaagggcgc
     8761 catgaaggcc atgccgaacc tcatggagac agaactggag aacttcggcg cctcggtcgt
     8821 cgaggacttc acgtggaagc aactcctcga caccgatagc tgcacgatgt gtggccgctg
     8881 cacctcggtg tgtcccgcgc atgcgacgg
//
LOCUS       DAOOMA010000028         8902 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_9183_length_8902_cov_4.386233, whole genome shotgun sequence.
ACCESSION   DAOOMA010000028 DAOOMA010000000
VERSION     DAOOMA010000028.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 8902)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8902)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8902
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9183_length_8902_cov_4.386233"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            118..1671
                     /locus_tag="PLP95_01605"
     CDS             118..1671
                     /locus_tag="PLP95_01605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HPB44526.1"
                     /translation="MADDTASADAAGIHDSADGFEPDGAVLIAEGSGLVNLLNGFIIE
                     LREAGLPVSLSESIDAMEAVKHIPIEDRAAFKYALAATLVKNNAHWRAFETVFEVYFS
                     MRGRQYLIGDADGDELPGDLEDLLEQMSGAGEGDGDGHGGGGAGGDQMTPEELAEMLF
                     RALQRGDQAMMKAVARQSVRRYAGMEPGRPVGGTYYLYRTLRNLDLDGVLQRLVQQDT
                     DDSPEPLTNLEQRLQEDEFQQRIEQLKQEIEAEIRRRLVADRGVEAMAKTLRKPLPED
                     IDFMHASRDEMKLLQQALHPLTRKLAVRLARKRRHGRKGALDFRNTVRASLSFGGVPV
                     EPKFKYPRPAKPEIFVIADISGSVASFARFTLHLVNAIAGQFSRVRSFVFIDGIDEVT
                     HMFEGTEDIVEAIHRVNTEADVVWVDGHSDYGHAFEVFWSRWGKEIGPRTSVMILGDA
                     RNNYHASQSWVIKEIQHRARHVYWLNPEPRSYWDTGDSIVGEYATYCDGAFECRNLRQ
                     LERFVDNLA"
     gene            complement(1704..2195)
                     /locus_tag="PLP95_01610"
     CDS             complement(1704..2195)
                     /locus_tag="PLP95_01610"
                     /inference="COORDINATES: protein motif:HMM:NF022161.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2505 family protein"
                     /protein_id="HPB44527.1"
                     /translation="MVFNVNQSFDASASEVFDCYESARFVGRLGPLGPLGEAQLVDRT
                     ERGDRTVVRTRMTFVADLPAAARAIVDRDKLAWIEEATYDRPSLTATVRFLPDHYANK
                     FEAAASTKFYDLGTGSVRAVSGDLKVRVPLVGRQAERAIVSGLVEYLESEAVVVARHL
                     AEV"
     gene            complement(2262..3161)
                     /locus_tag="PLP95_01615"
     CDS             complement(2262..3161)
                     /locus_tag="PLP95_01615"
                     /inference="COORDINATES: protein motif:HMM:NF019829.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PaaX family transcriptional regulator C-terminal
                     domain-containing protein"
                     /protein_id="HPB44528.1"
                     /translation="MNPTIVSDSTGDDPGIPTRLMVMGMAKRNGDIITAELAPVMDAC
                     GLSPDQVRSCLRRLVNEGLFERDGEGRDSRYHATEAGLAELRSIGDRTVLAYAQDHAG
                     RGWDRRWRIVAFAIPESMRASRDRFRDRLLALGAAAVHNGLYVAPHRWDTEVRAEISR
                     LGIGAYVTMATTDDLEIGGTSNPRDLAGRLWPLEQIAERYRDFIATYESVPAELEAMM
                     KRGERIAEHDFLPGALQIAIRFMRCFEADPLLPPELLPKPWPGRDAREVLVRCRKLGL
                     LTREEKGGPAMFSVFDEAISALP"
     gene            complement(3263..3967)
                     /locus_tag="PLP95_01620"
     CDS             complement(3263..3967)
                     /locus_tag="PLP95_01620"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: protein motif:HMM:NF002537.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004604 - phosphoadenylyl-sulfate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0019379 - sulfate assimilation,
                     phosphoadenylyl sulfate reduction by
                     phosphoadenylyl-sulfate reductase (thioredoxin) [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenylyl-sulfate reductase"
                     /protein_id="HPB44529.1"
                     /translation="MVESSIAIPSPRFVVAEFSDDELAEINKEFESAPASKIVRWAVD
                     SFGPHLALAASMADALLIDIATKVDPAIEVVFIDTGYHFPETIETVERIRRHYGLNLR
                     IMTVAPHDEELWVKDPENCCSAVKVGQLDRALNGKAAWMSGLRRDEAATRANAPIVGR
                     DPRGLIKVNPIANWTQLDCDGYLADNNVPHNPLLDQGYASIGCWPCTSPVAEGEDARS
                     GRWAGSAKTECGLHLA"
     gene            complement(3967..5814)
                     /locus_tag="PLP95_01625"
     CDS             complement(3967..5814)
                     /locus_tag="PLP95_01625"
                     /EC_number="1.8.7.1"
                     /inference="COORDINATES: protein motif:HMM:NF013259.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0050311 - sulfite reductase (ferredoxin)
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite/sulfite reductase"
                     /protein_id="HPB44530.1"
                     /translation="MTIIEIDPAASQAAQNLSPTNPSRDYEVPDDMAGDIEETIAKFD
                     RMLALHLAGEIEEDTFRVFRLNNGIYGQRQGGTNQMVRVKSPYGRLNAEQLDLMARVA
                     DEYSRGWGHITTRQNFQFHYVELTRVPDLLREFASVGMTTREACGDTVRNVEGCHLAG
                     ACPFEVLDVSAWAEAAFRYFLHHPFAQRLPRKFKINFSGCDTDCGQAMFNDAGVIAVT
                     RTLDDGTVEAGFRVFVAGGLGANPHAAFALEDFTPREQLMETLEAILRTFEQTGKRNN
                     KLRARLKWVAAELGEEELRNRILASRRLLPAAAGWPGGVPEVVNEWGDSPAGRSHEVA
                     ATPMGQGTPVQLLSSDPYQRWDQANVVRGTANGTVSAYAWARLGDVTSDQFRGLAAIV
                     REFDLEVRISNRQNLVFRGLTEEQLPALYERLVAIGMQQPGAELSRDVVACPGADTCN
                     IAVTQSRGLAEAIGVALEEAGLAEVGGIRTNISGCTNSCGQHHISDIGFHGAERRAHG
                     KAAPGYQMLLGGYVGQERIHFGERALRLPAKAAPEAVVRVVGRFNEERISGEDFRSWL
                     ERSGGTKAIAATLKDLDVFPTPEENPDFYVDYDETGPFEVEIGESECAV"
     gene            complement(5914..6435)
                     /locus_tag="PLP95_01630"
     CDS             complement(5914..6435)
                     /locus_tag="PLP95_01630"
                     /inference="COORDINATES: protein motif:HMM:NF022281.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2752 domain-containing protein"
                     /protein_id="HPB44531.1"
                     /translation="MEPASSEPAPHEPHDHESTPHEPAAAVAVPLPSALRPPSWRLPA
                     LATAASVVAVGAIAMRDPAVEGNWPSCPFFAMTGRFCPGCGSMRAIHALAHGDLTRAL
                     HSNVLLVVAVPLMTYLLIRAWERGLRGSFESKLPDPMATRWAPMVTLVSLASFWLVRN
                     LPGFEFLTPSFIG"
     gene            6470..8224
                     /locus_tag="PLP95_01635"
     CDS             6470..8224
                     /locus_tag="PLP95_01635"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HPB44532.1"
                     /translation="MQPGVDPADISSDPESASDRAPKWWQSTSIYQIYPRSFADSNGD
                     GIGDLEGIISRLDYLADLGVGAIWVSPFFTSPQRDVGYDVADYRNVSPEYGTIDDAQR
                     LIDEAHARGLRVLFDLVLNHTSDQHLWFVESRSSRTNPKADWYVWADARTDRLGRRRP
                     PNNWRSELQVERAWRWCPEREQYFLASFLDFQPDLNWRNPAVRAEMFDMVRMWLGRGV
                     DGFRLDIFGSIMQDAALRNNPPLPTVVAGLPRLQRPVHTLNTTENFELAADLREVCDE
                     FGPGERVLLGEVFGDEDTLARYVQVGDRPGLHVVFLFEFLHASYSAKRYRSLIERFER
                     RFPAPQQPTYVVENHDRARSMSRLGGDERKARVLATLMLTLRGIPVIFQGQEVGMTNK
                     YIPLREAVDPIPRVFRRILHEPLNRRLPERLNRDEVRVPMQWSDEPGVGFTDPDVTPW
                     LRYGDDAATHNVSVQNEDPASLLNLYRELYHLRREYPAIAHGSLELVPVDGGDEVIAY
                     RRGDPDQPEAGNVLVVCNLGDTGAAHRVEAGAAMLVRTDDSVALVQGLNDERWLNLPP
                     HSAAVLSGSVLGGAPHRR"
     gene            complement(8275..>8902)
                     /locus_tag="PLP95_01640"
     CDS             complement(8275..>8902)
                     /locus_tag="PLP95_01640"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020378134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006635 - fatty acid beta-oxidation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HPB44533.1"
                     /translation="MNAVRAIDEGRFANEIVPVTLPDGSQFTTDEHPRRNSSLEKLAT
                     LPVLNPQNDWATTTAGNSSGLNDAAAALTLVSPEFAKAHGLKPRAIIRGWASVGLAAE
                     ETGLTPEPAMRKALAKAGLSVADLQAVEINEAFASVAVAATKRLGLDPAIVNQNGSGC
                     SLGHPIGCTGARMIVTMLNELDRLDAQFGAVAMCAAGGMGSATVIERV"
BASE COUNT         1456 a         2843 c         3090 g         1513 t
ORIGIN      
        1 ccgtcggaac cgccagcatc ctgctcaagt accagtccga catcgcgaag gcgtcgaagg
       61 aattcgcgaa cgagggctcg agcttgcgca agggcggccc gctcagcgag gcctgacatg
      121 gccgacgaca cggcctcggc cgacgctgcc ggtatccacg actccgcgga cgggtttgag
      181 cccgacggtg cggtgctcat cgccgagggc agcggactgg tcaacctgct caacgggttc
      241 atcatcgagt tgcgcgaggc cggccttccg gtgagcctca gcgaatccat cgatgccatg
      301 gaggccgtca agcacattcc gatcgaggac cgcgccgcgt tcaaatacgc gctcgcggcg
      361 acgctggtga agaacaacgc ccactggcgc gcgttcgaga ccgtgttcga ggtgtacttc
      421 tcgatgcgtg gtcgtcagta tctcatcggc gacgccgacg gcgatgagct gcccggcgac
      481 ctcgaggacc tgctcgagca gatgagcggc gccggcgagg gcgacggcga cggtcatggc
      541 ggcggaggcg ccgggggcga tcagatgacc cccgaagaac tcgccgagat gctgtttcgg
      601 gccttgcagc gcggcgatca ggcgatgatg aaagctgtcg cccgccagtc ggttcgacgc
      661 tacgcgggga tggagcccgg tcgtccggtg ggcggcacgt actacctgta tcgcacgctt
      721 cggaacctcg atctcgacgg cgtgctgcag cgcttggttc aacaggacac cgacgattca
      781 cccgagccgt tgaccaacct cgaacagcgc cttcaagaag acgagtttca gcagcgcatc
      841 gaacaactca aacaggagat cgaggcggag atccgccggc ggctcgtcgc cgaccgtggt
      901 gtcgaggcga tggcgaagac gctgcgcaag ccactgcccg aggacatcga cttcatgcac
      961 gcgagccgtg acgagatgaa gctgctgcaa caggcgctgc accccctcac ccgcaagctc
     1021 gcagttcgtc tggcgcgaaa acgccgtcat ggtcgcaagg gtgcgctcga ctttcgaaac
     1081 accgtccgag cctcgctgag cttcggcggc gttcccgtcg agccgaagtt caagtacccg
     1141 cggccggcca aacccgagat cttcgtcatc gccgatatct ccgggtcggt cgcgagtttc
     1201 gcccgcttca cgttgcacct cgtcaacgcc atcgccggtc agttcagccg agtccgctcg
     1261 ttcgtcttca tcgacggtat cgacgaggtg acccacatgt tcgaaggcac cgaggacatc
     1321 gtcgaggcca ttcaccgggt caataccgag gccgacgtcg tgtgggtcga tggccactcc
     1381 gactacggcc atgcgttcga agtgttctgg tcacggtggg gcaaggagat cgggccgcgc
     1441 accagcgtga tgatcctcgg cgacgcccgc aacaactatc acgcctcgca gagttgggtc
     1501 atcaaggaga tccagcaccg cgcccgtcac gtgtattggc tcaaccccga gccgagaagc
     1561 tactgggaca ccggcgactc gatcgtgggg gagtacgcga cgtactgcga tggggccttt
     1621 gagtgtcgca atctgcggca actggagcgg ttcgtcgaca atctcgcgta gccgggtgcc
     1681 gtcggtcgcc ggggaccagc ggttcagacc tcggccaggt ggcgggcgac gacgaccgct
     1741 tccgactcga ggtactcgac gaggccggac acgatcgccc gttcggcctg gcgaccgacg
     1801 agcggcaccc gcaccttcag gtcgcccgac accgcccgga ccgaaccggt tccgaggtcg
     1861 tagaacttcg ttgatgccgc cgcctcgaac ttgttggcgt agtggtcggg caggaaacga
     1921 accgtcgcgg tgagcgacgg tcggtcgtag gtcgcctcct cgatccaggc gagcttgtcg
     1981 cggtcgacga tggcccgggc cgccgccgga aggtcggcca caaaggtcat ccgcgtgcgc
     2041 acgaccgtgc ggtcgccacg ttccgtccgg tcgacgagtt gcgcttcgcc gagtggcccc
     2101 aacggtccca gccgtccgac gaaccgagcg gattcgtagc agtcgaacac ctcgctggcc
     2161 gaggcatcga agctctggtt gacattgaac accatgggct acttcctacc cgatcccggg
     2221 tggatcctgc cgtcgttgtg tgatcccgtc ggccggaccg gtcagggcag tgcggagatg
     2281 gcctcgtcga aaaccgagaa catggcaggt ccgcccttct cctcgcgggt gagcaggccc
     2341 aacttgcggc accggaccaa cacctcgcgg gcgtcgcggc cgggccacgg cttcgggagc
     2401 agttcgggtg gaagcagtgg gtcggcttcg aagcagcgca tgaagcggat ggcgatctgc
     2461 aaggcacccg gaagaaagtc gtgttcggcg attcgttcac cccgcttcat catcgcttcg
     2521 agttccgcgg gcacggactc gtaggtggcg atgaagtcgc ggtaacgctc ggcgatctgc
     2581 tcgaggggcc acagacgacc ggccaggtcc ctcggattgg acgtgccgcc gatctcgaga
     2641 tcatcggtcg tcgccatggt gacgtaggcg ccgattccga gccgtgaaat ctcggcgcga
     2701 acttcggtgt cccaccgatg gggggccaca tacaacccgt tgtggacggc cgccgcgccg
     2761 agtgccagca gtcggtcgcg gaaccggtcc cgagaggcgc gcatcgactc cgggatggca
     2821 aaggcgacga tgcgccagcg ccgatcccag ccgcggccgg cgtggtcctg tgcataggcc
     2881 aatacggtgc ggtcgccgat cgagcggagc tccgcgagcc cggcctcggt cgcgtggtag
     2941 cggctgtcgc gaccttcgcc gtcgcgttcg aagagccctt cattcacgag gcgccgcaga
     3001 cacgaccgga cctgatcggg ggagagcccg cacgcgtcca tgaccggggc gagttccgcg
     3061 gtgatgatgt cgccgttgcg tttggccatc cccatgacca tcagtcgggt cgggatgccc
     3121 gggtcgtcac cggtcgaatc tgaaacgatc gttgggttca cgaccacatc gtggccaacg
     3181 accggggtga tgtcaacgtg gagcgaggcc gcaatttcca ccggcgcggc ccgctggtcg
     3241 accgggcact agtgcggtgg cactaggcca gatggaggcc gcactcggtc ttggcgctgc
     3301 cggcccaccg acccgagcga gcatcttcgc cctcggcgac cgggctggtg cagggccaac
     3361 aaccgatcga ggcgtagccc tggtcgagca gcgggttgtg ggggacgttg ttgtcggcca
     3421 ggtacccgtc gcagtcgagt tgggtccagt tcgcgatcgg gttgaccttg atgagcccgc
     3481 gagggtcacg accgacgatt ggcgcgttgg ccctcgtggc ggcttcgtcg cggcgcagcc
     3541 cactcatcca cgcggccttt ccgttgagcg cgcggtcgag ttggccgacc ttgaccgccg
     3601 agcagcagtt ctcgggatcc ttgacccaca gttcctcgtc gtgcggagcg acggtcatga
     3661 tgcgcaggtt caacccgtag tgacgccgga tccgttcgac cgtctcgatc gtttcgggga
     3721 agtggtaccc ggtgtcgatg aacacgacct cgatcgcggg atcgaccttg gtggcgatgt
     3781 cgatgaggag cgcgtcggcc atcgacgccg ccagcgcgag gtgcgggccg aagctgtcga
     3841 ccgcccagcg cacgatcttc gaggcaggcg cggattcgaa ctccttgttg atctccgcga
     3901 gttcgtcgtc gctgaactca gcgacgacga atcgcggcga tgggatggcg atggatgact
     3961 cgaccatcag actgcgcact ccgactcgcc gatttcgact tcgaacggtc cggtttcgtc
     4021 gtagtcgacg tagaagtcgg ggttctcctc gggggtgggg aacacgtcga gatccttcaa
     4081 ggtcgcggcg atcgccttgg ttccgcccga gcgctcgagc cagctgcgga agtcctcgcc
     4141 agaaatgcgc tcctcgttga atcgcccgac cacccgcacg acggcctcgg gcgccgcctt
     4201 ggcgggaagg cgcagggcgc gctcgccgaa gtggatgcgc tcctgaccga cgtaaccgcc
     4261 gagcagcatc tgatagccgg gtgccgcctt accgtgcgct cggcgttcgg cgccgtggaa
     4321 gccgatgtcg gagatgtggt gttgcccgca ggagttggtg caaccgctga tgttggtgcg
     4381 gatgccgccg acctcggcaa ggccggcctc ttcgagcgcc acgccgatcg cttcagcgag
     4441 accgcgcgat tgggtgacgg cgatgttgca cgtgtcggca ccggggcatg cgaccacgtc
     4501 gcgtgacagt tccgcccccg gctgttgcat gccgatggcg acgaggcgct cgtagagggc
     4561 cgggagttgt tcctcggtga gtccccgaaa cacgagattc tggcgattcg agatgcggac
     4621 ctcgaggtcg aactcgcgca cgatggcggc cagcccgcgg aactgatcgg aggtgacgtc
     4681 gccgaggcgg gcccaggcgt aggcgctcac cgtgccgttc gccgtgccgc gcacgacgtt
     4741 ggcctggtcc caacgctggt acgggtcgct cgacaggagt tgcaccgggg tgccctggcc
     4801 catcggcgtg gcggctacct cgtgtgagcg gccagcgggg gagtcacccc attcgttgac
     4861 gacctcggga acgccgcccg gccagccggc agcggccggc aacaagcgcc gactggcgag
     4921 gatgcggttg cgcagttcct cctcgccgag ttctgccgcc acccacttga ggcgggcgcg
     4981 gagcttgttg ttgcgtttcc cggtctgttc gaaggtgcgc aggatggcct cgagggtctc
     5041 catcagctgc tcgcgtgggg tgaagtcctc gagtgcgaac gccgcgtgcg ggtttgcccc
     5101 gagccccccg gcgacgaaga cgcggaatcc ggcctcgacc gttccgtcgt cgagggtgcg
     5161 ggtgacggcg atgacgccgg cgtcgttgaa catcgcctgg ccgcaatcgg tgtcgcaacc
     5221 cgagaaattg atcttgaatt tgcgggggag gcgctgggcg aacgggtggt ggaggaagta
     5281 acgaaacgcc gcctcggccc aggcgctgac gtcgaggacc tcgaaggggc aggcgccggc
     5341 gaggtggcat ccctcgacgt tgcgcaccgt gtcgccgcag gcctcgcggg tcgtcatgcc
     5401 caccgaggcg aattcgcgca ggagatccgg gacccgggtg agttcgacgt agtggaactg
     5461 gaagttctgg cgggtggtga tgtggcccca cccgcgcgag tactcgtccg cgacgcgggc
     5521 catgaggtcg agttgttcgg cgttgaggcg gccatagggc gacttcaccc gcaccatctg
     5581 gttggtgccg ccctgacgtt ggccgtaaat gccgttgttg aggcggaaca cgcggaaggt
     5641 gtcctcctcg atctcgccgg cgaggtgcag ggcgagcatt cgatcgaact tggcgatcgt
     5701 ttcctcgatg tcgccggcca tgtcgtcggg gacctcgtag tcccgggatg ggttcgtcgg
     5761 cgagagattc tgggctgcct gggatgcagc gggatcgatt tcgatgatgg tcacgtcggt
     5821 ctccgagggg tgaagtcctg ccagtgtacg ttaatcccga ccttctgagt caagaattgc
     5881 gaactggtcg gttactcgcg gaggtgcgac ggttcagccg atgaaactgg gggtgaggaa
     5941 ctcgaacccg ggcaggttgc gcaccaacca gaacgacgcg agcgaaacga gggtgaccat
     6001 cggcgcccag cgcgtcgcca tcggatccgg cagcttcgac tcgaacgagc cgcgcaatcc
     6061 acgttcccac gcccggatca ggaggtaggt catcaagggg acggcgacga cgagcaacac
     6121 gttggaatgc agcgcgcggg tgaggtctcc gtgggcgagc gcgtggatgg cccgcatcga
     6181 cccgcatccg gggcagaacc gcccggtcat cgcgaagaac gggcacgatg gccagttgcc
     6241 ctcgaccgcc ggatctcgca tcgcaatggc ccccacggcc accaccgacg cagcggtggc
     6301 gagcgcgggc agacgccaac tggggggtcg caacgccgac ggaagcggca cggcgacggc
     6361 ggccgccggt tcgtgcggtg tggattcgtg gtcgtgcggt tcgtggggcg cgggttcgct
     6421 ggatgcaggt tccatgagag ggctttcgct cacacgttag ggtctggcca tgcaacccgg
     6481 cgtcgaccct gccgacattt cgtccgaccc cgaaagcgct tctgaccgtg cacccaaatg
     6541 gtggcagtcg acgagcattt accagatcta tccgcggtcc ttcgccgact cgaacggcga
     6601 cggcatcggc gatctcgagg gcatcatctc ccggctcgac tacctcgccg acctcggcgt
     6661 gggggcgatc tgggtgtcgc cgttcttcac ctcgccccaa cgtgacgtgg ggtacgacgt
     6721 cgccgactat cgcaacgtgt caccggagta cggcacgatc gacgatgccc agcgcctgat
     6781 cgacgaggcc cacgctcgcg ggctgcgggt gctgttcgac ctcgtgttga accacacctc
     6841 cgaccagcac ctctggtttg tcgagtctcg gtcctcacgc acgaacccga aggccgattg
     6901 gtacgtgtgg gccgacgctc gcacggatcg gctcggtcgt cgccggccgc cgaacaactg
     6961 gcgttcggag ttgcaggtcg aacgcgcctg gcggtggtgc cccgaacgcg agcagtactt
     7021 cctcgccagc ttcctcgatt tccagcccga cctgaactgg cgcaacccag cggtgcgcgc
     7081 cgagatgttc gacatggtgc ggatgtggct cgggcgcggg gtcgacgggt tccgcctcga
     7141 catcttcggt tcgatcatgc aggacgccgc gctgcgcaac aatccgccgt tgcccaccgt
     7201 cgtcgccggg ttgccccgcc tgcagcgccc ggtccacacc ctcaacacca cggagaactt
     7261 cgagttggcc gccgaccttc gcgaggtgtg cgacgagttc gggccggggg agcgggtgtt
     7321 gctcggcgag gtgttcggcg acgaggacac gctggcgcgc tacgtgcagg tcggcgatcg
     7381 gccggggctg cacgtggtgt tcctcttcga attcctccac gcgagctatt cggcgaagcg
     7441 ctatcgcagt ctgatcgagc ggttcgaacg acggtttccc gcaccccagc agccgaccta
     7501 tgtggtggag aaccacgacc gggcgcggtc gatgtctcgc ctcggcggcg acgagcgcaa
     7561 ggcccgggtg ttggccacgc tcatgttgac gctgcgcggc atcccggtga tttttcaggg
     7621 ccaagaggtc ggaatgacca acaagtacat cccgttacgc gaggccgtcg acccgatccc
     7681 gcgggtgttt cgccgcatcc tgcacgagcc gctcaaccgg cggttgcccg agcggctgaa
     7741 ccgtgacgag gttcgggtgc cgatgcaatg gagcgacgag cccggcgtcg gcttcaccga
     7801 tcccgatgtc accccgtggc tgcgctacgg cgacgatgct gcgacacaca acgtgtcggt
     7861 gcaaaacgag gacccggcgt cgctgctcaa cctgtatcgc gagctgtatc accttcgtcg
     7921 cgagtatccg gcgatcgctc acgggtcgct ggagttggtt ccggtcgacg gcggcgacga
     7981 ggtgatcgcc tatcggcgcg gcgatcccga tcagcccgaa gccggcaacg tgttggtggt
     8041 gtgcaacctc ggcgataccg gcgctgccca tcgggtcgaa gcgggtgcgg cgatgctggt
     8101 tcgcaccgac gactcggttg ctctggtgca gggcctcaac gacgaacgat ggctcaacct
     8161 gccaccccac agcgccgccg tgctgagcgg gtcggtgctg ggcggggcgc cgcatcgccg
     8221 ttgatggtcg tggccccgcc ggggctacca gcgaggccac gacgctccgg gcgatcagac
     8281 gcgttcgatg acggtggccg aacccatgcc gccggcggca cacatggcga cggcgccgaa
     8341 ctgggcgtcg aggcggtcga gttcgttgag catggtgacg atcatgcggg caccggtgca
     8401 gccgatcggg tgaccgagcg agcagcccga accgttctgg ttcacgatcg ccgggtcgag
     8461 gccgaggcgc ttggtggcgg cgacggcgac cgaggcgaac gcctcgttga tctcgacggc
     8521 ctgcaggtcg gcaacgctca ggccggcctt ggcgagggcc ttacgcatag cgggctcggg
     8581 ggtgagcccg gtctcctcgg cggcaagccc gaccgaagcc cagccgcgga tgatggcgcg
     8641 gggcttgagg ccatgcgcct tggcgaattc gggggacacg agcgtgagcg cagcggcggc
     8701 gtcgttgagg cccgaggagt ttccggccgt ggtggtcgcc cagtcgttct ggggattcag
     8761 caccggcagt gtcgcaagct tctcgaggct cgagttgcgg cgggggtgct catcggtcgt
     8821 gaactgcgag ccgtcgggca gggtcaccgg cacgatctca ttggcgaacc tgccctcgtc
     8881 gatggcgcgc acggcgttca tg
//
LOCUS       DAOOMA010000029         8624 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_9585_length_8624_cov_3.257556, whole genome shotgun sequence.
ACCESSION   DAOOMA010000029 DAOOMA010000000
VERSION     DAOOMA010000029.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 8624)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8624)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8624
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9585_length_8624_cov_3.257556"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..191)
                     /locus_tag="PLP95_01645"
     CDS             complement(<1..191)
                     /locus_tag="PLP95_01645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB44534.1"
                     /translation="MLTQRIASPASRRRWLLGAALAALAAFVLTRPAGAAAGAASSAV
                     AGGSEAAQLKLWQAIVLGVV"
     gene            complement(327..809)
                     /locus_tag="PLP95_01650"
     CDS             complement(327..809)
                     /locus_tag="PLP95_01650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003054718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HPB44535.1"
                     /translation="MANVFDFTATDIDGAATPLSTWNGDVLLITNVASKCGFTPQYEG
                     LEALHRDFADRGFEVLGFPCDQFAHQEPGNEEEIKNFCSLTYDVTFPMFSKIDVNGPD
                     THPLFEWLKDQRSGLLGGRIKWNFTKFLIGRDGQVIKRYAPTTKPEQIRSDIDAALTR
                     "
     gene            887..3064
                     /locus_tag="PLP95_01655"
     CDS             887..3064
                     /locus_tag="PLP95_01655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020376951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamate-ammonia ligase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase III"
                     /protein_id="HPB44536.1"
                     /translation="MGGSTDRRRAINAIAHWTPPAPFFNDSDTPGELFGANVFSKAVM
                     RSRLPKNIYQSVVATIEESKPLDHTIADVVASAMKDWAMEKGATHYAHVFYPLTGLTA
                     EKHDSFLEPDGDGGVISEFTGKTLVQGEPDASSFPNGGLRGTFEARGYTGWDVTSPAY
                     ILENPNGNTLCIPTVFVSWTGEALDKKLPLLRSRQALDGAARRVLELFGHTDIDPIQS
                     YAGAEQEYFLIDRNLYLQRPDLMSTGRTLLGCPPPKGQEFDDHYFGSIPDRVLAFMMD
                     VDRELFKLGIPAKTRHNEVAPGQFEIAPVFEIANLAHDHQQLLMSTLRRVAEKYDLEC
                     LLHEKPFAGVNGSGKHVNFSFGNKAQGNLLNPGDTPHENAQFLVFCAAVIRGVHRHGG
                     VLRAVVASARNDHRLGANEAPPAIISIFLGDQLVDVFEQIKGGGATESKGRQALVVGV
                     DTLPPLPADPGDRNRTSPFAFTGNRFEFRAPGSSQSISGPMVAINTIVAESLDYVANE
                     LESRLSKGDDLNSAIQSVLQQIMVDHDAVLFNGDGYSDDWQVEAERRGLLNLRTSADA
                     LPQFGTSEAIALFSGYEVLNERELASRQEVYLEQYVLGVEVEARQLLEMCNTLIYPAA
                     ARYQAQLARGLVDLAALGLDGDREVINIVVAGIHQLRAATAALAASLAEAPHGDTQQA
                     ADHCRDRLLPAMERLRAVVDGMENEVADDLWPLPTYQEMLFIL"
     gene            complement(3131..4438)
                     /locus_tag="PLP95_01660"
     CDS             complement(3131..4438)
                     /locus_tag="PLP95_01660"
                     /inference="COORDINATES: protein motif:HMM:NF018448.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HPB44537.1"
                     /translation="MDTPHPRQVLDLALAIGEILLAEGMSASDLVASLTTVTKAYGIE
                     RAFFDVTYTQLSATCVPDLGETPITAFRMVHQRSLDFTRVRALDQLLQDIGQGMPFGD
                     AVATFEALRDAPAPYASGWTTIGDATLGAGVVLLFTTNWRDILLSFLICLAISRLVRL
                     LDAYEVPVLFQQGAGAAVAGLFAATAAELGRRDVWPFVGSTPALLVVGGVVVLVAGIT
                     AVGAVLDAIDEFYVTASARMFETLMRTVGIVGGLLLGLRIAEAAGFAMTVRTDAIPLG
                     PFPAQVLGAAMIAYGFALFAYADRTTRLLAATMGVTAWLSYSLAVFVGAGETVSNAIG
                     ALAATFISTLIVRHTTVPSFALITAALLPLVPGLALYSGLLMTVGTTADTANVAGGSQ
                     GVLSALMVALGIAAGASLGTFLGRPIADRVRTRRRLSDANPIS"
     gene            complement(4467..5786)
                     /gene="efeB"
                     /locus_tag="PLP95_01665"
     CDS             complement(4467..5786)
                     /gene="efeB"
                     /locus_tag="PLP95_01665"
                     /inference="COORDINATES: protein motif:HMM:TIGR01412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0033212 - iron import into cell [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake transporter
                     deferrochelatase/peroxidase subunit"
                     /protein_id="HPB44538.1"
                     /translation="MSEDVTPRVSRRSVLTGGAAAAVGLGVGAGAGVAIAKSGSSDEG
                     GSGKGGSGEGGASHDIVEFRGAHQAGIITPAQDRLYFAAFDVITDDRTELAAMLAKWT
                     EAAERLTRGEEHGRYGATGGPALAPPEDTGEALGLPASALTLTFGFGRSLFLTPDGID
                     RFGLASRLPEALIELPNFPADLIDRAISDGDLCVQACSNDPQVAFHAVRNLARIGFGT
                     VSLRWSQLGFGRTSSTSTAQDTPRNLFGFKDGTANLKAEESAELDRHLWVQPDDVGAD
                     QAWMAGGTYLVARRINMHIETWDRSSLREQETIIGRTKLSGAPLSGGEEFTEPDFAIT
                     GRDDAPLIDMASHVALAHPQNNDGVRMLRRGYNFTDGSNDLGRMNAGLFFIAFMRDPV
                     TQYVPMQTTLARDDLLSEYLQHTGSGLYAVPPGLSEGESLGEALLSD"
     gene            complement(5783..6967)
                     /locus_tag="PLP95_01670"
     CDS             complement(5783..6967)
                     /locus_tag="PLP95_01670"
                     /inference="COORDINATES: protein motif:HMM:NF020932.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imelysin family protein"
                     /protein_id="HPB44539.1"
                     /translation="MNHRYRAFAAAVALLGVTACTDNNPDTASGTDGTDGTAGGAPVA
                     VSSTATECTLSATEAPSGNLVFEVTNDGDDVTEFYLLASDGLRIVGEVENIGPGLTRK
                     LVVLAQPGDYITACKPGMVGEGIRADFTVNDSGNEVGPTGERAELLSVATTQYAAYVK
                     DQAEQLLAKTREFADLYRNGDDDAARALYANARMHWERIEPVAESFGDLDPRLDLREA
                     DLEDGQEWTGWHTIEKDLWPPTDGSYTALSTQRRNELADQLVADTEELVERVGGLSFT
                     ADQLGNGAKELLDEVATGKVTGEEEAWSHTDLWDFQANVDGARIAFEDLRPALDTTDA
                     ELGTTLEKRFTELQSLLDQYKTTDGWKYYNELTEAEVKGLSDAVNALSEPLSELTAAV
                     VL"
     gene            complement(6964..7866)
                     /locus_tag="PLP95_01675"
     CDS             complement(6964..7866)
                     /locus_tag="PLP95_01675"
                     /inference="COORDINATES: protein motif:HMM:NF015213.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FTR1 family protein"
                     /protein_id="HPB44540.1"
                     /translation="MMSNFLIGLREGLEASLVIGILYAYLVRTDRRHLLRPMWAGVAI
                     AVVVSLGFGAVLTFGPKGLSFTAQEAIGGFLSLVAVGFITWMIFWMARTARTLKTDLE
                     GRLSLAAEGGPAAVTIMAMLAVGREGLETALFVWAGVQASGNNTEPLLGALIGIATAI
                     VLGFLITRGAVRINLSKFFTWTAFALIIVAAGILSYGIHDLQEAGILPGLNNLAFDVS
                     DQIPPTSWYGTLLKGTINFSPATTWLEAIAWSVYLVVVIPLFVRTIRQSTGPSQTTRT
                     NPATPTTGSSAPTSPQSFQPEVTT"
     gene            8090..>8624
                     /locus_tag="PLP95_01680"
     CDS             8090..>8624
                     /locus_tag="PLP95_01680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB44541.1"
                     /translation="MVSGALDPNVANPHGVSMLRLGDLTAGPIATVAPGGVVTLPGDS
                     STRWSWWIGAEDGWHRPAHEPTTQDAALDGAPGLRTTARIPGGDFVGTATAVRFDALD
                     GPTLLFELDNASAVPVAVAFVVESDRPITVRDGGIDVDGVGALRLTRVAAASLSASSA
                     DALVTALVEAGIAAATTG"
BASE COUNT         1451 a         2750 c         2974 g         1449 t
ORIGIN      
        1 acgacgccga ggacgatggc ctgccacagc ttcagctgtg cggcctcgga tccgcccgca
       61 accgccgatg aggcggcacc cgccgcagcg cccgccggtc gagtgagcac gaaggcggca
      121 agggccgcga gggctgcacc gagcagccac cgtcgtcggc tcgcagggga agcgattcgt
      181 tgagtgagca cccaggcacc ctatcgagtc acctcgaaac tccggttgct ttggcgtccc
      241 aatctcaccc gcagtgggcg ttctgtaggt aattccggtg gtcatggcaa ccgcgattac
      301 ctacagaacg gcgtggggcg ggtcggctag cgggtgaggg cggcgtcgat gtcggaacgg
      361 atctgttcgg gcttagtcgt cggcgcatac cgcttgatga cctgtccgtc tcggccgatg
      421 aggaacttcg tgaagttcca cttgatgcgt ccgccgagca atccggaacg ttgatccttg
      481 agccactcga acaacggatg ggtgtcgggg ccgttcacgt cgatcttcga aaacatcgga
      541 aaggtcacgt cgtaggtgag cgagcagaag ttcttgatct cctcctcgtt gcctggttcc
      601 tggtgggcga actgatcgca cggaaagccg agcacctcga acccgcggtc ggcgaagtcg
      661 cggtgcaacg cctcgagccc ctcgtactgc ggcgtgaacc cgcatttgga cgcgacgttg
      721 gtgatcagca gcacgtctcc gttccaggtc gacagcggtg tggcggctcc gtcgatgtcg
      781 gtggcggtga aatcaaagac gttggccatg atggggctcc cctcggaaac attgcgttaa
      841 caaaacgccc ataggctgtg gccgtccctg acgacaggag tgttctatgg gtggcagtac
      901 ggaccgtcgg cgcgcgatca acgctattgc gcattggacc cctccggcgc cgttcttcaa
      961 cgacagcgac acccccggtg agctgttcgg tgcgaacgtg ttctccaagg cggtcatgcg
     1021 ttctcgcctt ccgaagaaca tctaccagtc ggtcgtggcg acgatcgagg agtccaagcc
     1081 cctcgaccac accatcgcgg atgtggtcgc ctcggcgatg aaggactggg cgatggaaaa
     1141 gggcgccacg cactatgcgc acgtctttta cccgctcacg ggtctgaccg ccgagaaaca
     1201 cgacagcttc ctcgagcccg acggcgacgg tggcgtgatc tccgagttca ccggcaagac
     1261 cctcgtgcag ggcgagcccg atgcgtcgag ctttccgaac ggcggcctgc gcggcacctt
     1321 cgaggcccgg ggctacaccg gctgggacgt gaccagcccc gcctacatcc tcgaaaaccc
     1381 gaacggcaac acgctgtgca ttcccacggt gttcgtgtcg tggaccggtg aggcgctcga
     1441 caagaagttg ccgttgctgc gctcccgcca ggccctcgac ggcgcggcgc ggcgggtgct
     1501 cgaactgttc ggtcacaccg acatcgaccc gatccagagc tatgcgggtg ccgaacagga
     1561 gtacttcctg atcgaccgca acctctacct gcaacgcccc gacctgatgt cgaccgggcg
     1621 cacgttgctg gggtgcccgc cgcccaaggg ccaggagttc gacgaccact acttcggatc
     1681 gatccccgac cgggttttgg cgttcatgat ggatgtcgac cgcgagctgt tcaagctcgg
     1741 cattcccgca aagacccgcc acaacgaggt cgctcccgga caattcgaga tcgccccggt
     1801 gttcgagatc gccaacctgg cccacgacca tcagcagctt ctgatgtcga cgctgcgccg
     1861 ggtggccgaa aagtacgacc tggaatgtct gttgcacgaa aagcccttcg ccggggtgaa
     1921 cgggtcgggc aagcacgtga acttcagctt cggcaacaag gcccagggga acctgttgaa
     1981 ccccggcgac acgccccacg agaacgcaca gttcctcgtg ttctgcgcgg cggtgatccg
     2041 cggggtgcac cgccatgggg gcgtgttgcg cgccgtggtg gcctcggccc gcaacgacca
     2101 tcgcctcggc gcgaacgagg cgccgccggc gatcatttcg atcttcctcg gcgaccaact
     2161 cgtcgacgtg ttcgaacaga tcaagggcgg gggagcgacc gagtcgaagg gccgtcaggc
     2221 acttgtggtc ggcgtcgaca ccctgccgcc gttgcccgcc gatccgggcg accggaaccg
     2281 cacgagcccc ttcgccttca ccggcaatcg cttcgagttc cgtgctccgg ggtcctcgca
     2341 gtcgatctcg ggtccgatgg tcgccatcaa cacgatcgtg gccgaatcgc tcgactacgt
     2401 cgcgaacgag ttggagtcgc ggctgtcgaa aggcgacgat ctcaactcgg cgattcagtc
     2461 ggtgttgcaa cagatcatgg tcgaccacga cgccgtgctc ttcaacggcg acggctattc
     2521 cgacgattgg caggtcgagg ccgagcggcg aggcctgttg aaccttcgca ccagcgccga
     2581 tgcgctcccg cagttcggca ccagcgaggc gattgcgctg ttcagcggct acgaggtgct
     2641 caacgagcga gagttggcca gccgccagga ggtgtacctc gagcagtacg tgctgggcgt
     2701 cgaggtggag gcgcgacaac tgctcgagat gtgcaacacg ctgatctacc ctgcagcggc
     2761 gcgttaccag gcccaactcg cccgaggcct ggtcgacctg gcggccttgg gcctcgacgg
     2821 agaccgcgag gtgatcaaca tcgtcgtggc cggcattcat caactccgcg ctgcgacggc
     2881 ggccctggcg gcgtcgttgg ccgaggcccc gcacggagac acccaacagg cagcggacca
     2941 ttgccgcgac cggttattgc cggcgatgga gcgtctgcgg gcggtggtcg atgggatgga
     3001 aaacgaggtt gccgacgacc tgtggccgtt gccgacctac caggagatgc tcttcatcct
     3061 gtagccaccc gccggtatcc tgtagccacc cgccggcatc ggcggagcta ccggcagtgc
     3121 gcggcgcgcc tcaggagatc gggttcgcgt cgctgaggcg tcgtctcgta cggacccgat
     3181 cggcgatcgg tctaccgagg aaggtgccga gggaggcccc cgccgcaatg ccgagggcga
     3241 ccatgagcgc gctcagcacc ccttggcttc ctccggccac attcgcagtg tcggccgtcg
     3301 ttccgaccgt catcagtagg ccgctgtaca gggccagtcc gggcacgagc ggcagcaacg
     3361 ctgcggtgat gagcgcgaaa ctcggcacgg tggtgtggcg gacgatgagc gtggagatga
     3421 aggtcgcagc caacgcgccg attgcgttcg agacggtctc gccggcgccg acgaacacgg
     3481 cgagcgaata ggacagccac gccgttaccc ccatggtcgc ggccagcagg cgagtggtgc
     3541 ggtccgcata ggcgaacagg gcgaacccgt aggcgatcat ggccgccccc aacacctggg
     3601 ccggaaatgg tccgaggggg atggcatcgg tgcgcaccgt catcgcgaag ccggccgcct
     3661 ctgcgatgcg cagccccagg agcagtccgc cgacgatgcc gacggtgcgc atgagcgttt
     3721 cgaacatccg ggccgaagcg gtcacgtaga actcgtcgat cgcgtcgagc acagcaccga
     3781 cggcggtgat tccggcgacg agcacgacga ctccgccgac gacgagcagt gcaggggtcg
     3841 agccgacgaa tggccagacg tcgcgccggc caagttccgc tgcggtggcg gcaaacagcc
     3901 cggcgacagc ggctcccgcg ccctgttgga acagcaccgg cacctcatag gcgtcgagga
     3961 ggcgcacgag ccggctgatg gccaaacaga tgaggaacga caacaagatg tctcgccagt
     4021 tggtcgtgaa gagcaacacg acgccggcgc cgagggtggc gtcgccgatg gtggtccatc
     4081 ccgacgcgta cggggcgggt gcgtcgcgca gagcctcgaa ggtggcgacg gcgtcgccaa
     4141 agggcatccc ctggccgatg tcctgaagca gctggtcgag cgcccggacc ctcgtgaaat
     4201 cgagcgagcg ctggtgcacc atgcgaaacg cggtgatcgg ggtctcgccg aggtctggca
     4261 cgcaggtggc cgacagctgg gtgtaggtga cgtcgaagaa ggcccgctcg attccgtagg
     4321 ccttggtcac ggtcgtgagc gacgccacga gatcgctggc cgacatcccc tcggccagca
     4381 gaatctcacc gatcgccaaa gccagatcga gcacctggcg gggatgggga gtgtccatcc
     4441 acgccaacgt aacgtggtcg gcgccttcag tcggacagca gtgcctcgcc gagtgattcc
     4501 ccttcggaca gacccggcgg aacggcatac agcccggagc cggtgtgttg gaggtattcg
     4561 ctcaacaggt cgtcgcgggc cagcgtcgtt tgcatcggca cgtactgcgt caccggatcc
     4621 cgcatgaatg cgatgaagaa cagccccgca ttcatgcgtc cgaggtcgtt ggagccgtcg
     4681 gtgaagttgt agccgcgacg caacatccga actccgtcgt tgttctgcgg gtgagcgagc
     4741 gcgacgtgcg aggccatgtc gatgagcggg gcgtcgtcgc gtccggtgat cgcgaagtcg
     4801 ggttcggtga actcctcgcc gcccgacagc ggcgcaccgc tcagtttcgt gcgcccgatg
     4861 atggtctcct gctcgcgcaa cgacgaacga tcccaggtct cgatgtgcat gttgatgcgc
     4921 cgtgccacca gataggtgcc gcccgccatc cacgcctgat ccgctccgac gtcgtcgggt
     4981 tgcacccaca ggtggcggtc gagttctgcg ctctcctcgg ctttgaggtt cgccgttccg
     5041 tctttgaatc cgaagaggtt gcgcggggtg tcctgggcgg tcgacgtgga cgaggtccgc
     5101 ccgaagccga gctgtgacca acgaagcgag acggttccaa agccgattct ggcgaggttg
     5161 cgaaccgcat ggaacgcgac ctgggggtcg ttcgaacagg cctgcacaca caggtcgccg
     5221 tcgctgatcg cccggtcgat cagatcggcg gggaagttcg gcaactcgat gagtgcctcg
     5281 ggcaggcgcg aggccagtcc gaaacggtcg atgccgtcgg gggtgaggaa cagcgaacgg
     5341 ccgaacccga aggtgagggt gagggccgac gccgggaggc cgagcgcctc accggtgtcc
     5401 tcggggggag cgagtgccgg tccgccggtc gctccgtagc gcccgtgttc ctccccgcgg
     5461 gtcagacgct cggcggcctc ggtccacttc gccagcatcg ccgccagctc ggtccggtcg
     5521 tcggtgatga cgtcgaaggc cgcgaagtac aggcggtcct gggcgggggt gatgatgccg
     5581 gcctggtgtg cgccgcgaaa ctcgacgatg tcgtgtgaag cgccgccctc gcccgatccg
     5641 cccttgcccg atccaccctc gtcggaggat cccgacttcg cgatcgccac cccggcgccc
     5701 gcaccgacgc cgaggccaac ggcggccgcg gcgccgccgg tgagcaccga acggcgcgag
     5761 acgcgcgggg tgacgtcctc gctcacagca ccacggcggc cgtcagctcg ctgaggggct
     5821 cgctgagcgc gttgaccgca tcgctcaacc ccttgacctc ggcttcggtc aactcgttgt
     5881 agtacttcca accgtcggtg gtcttgtact ggtcgagcag gctctggagc tcggtgaaac
     5941 gcttctcgag cgtggtgccg agttcggcat cggtggtgtc gagcgccggg cgcaggtcct
     6001 cgaaggcgat ccgggccccg tcgacgttcg cctggaagtc ccacaggtcg gtgtgcgacc
     6061 aggcctcctc ctcgcccgtc accttgccgg ttgccacctc gtcgagcagt tccttggccc
     6121 cgtttccgag ttgatcggcg gtgaagctca ggccgccgac ccgttcgacg agctcctcgg
     6181 tgtcggccac gagttgatca gcgagttcgt tgcggcgttg cgtgctgagg gcggtgtagg
     6241 acccgtcggt cggcggccag aggtcctttt cgatcgtgtg ccagcccgtc cattcctggc
     6301 cgtcctcgag gtcggcctcg cgaaggtcga gccgagggtc gaggtcgccg aaggactctg
     6361 cgacgggctc gatgcgctcc cagtgcatac gtgcgttggc atacagcgct cgggcggcgt
     6421 cgtcgtcacc gttgcggtac aggtcggcga actctcgggt cttggccagc aactgctcgg
     6481 cctggtcttt gacgtacgcg gcgtattggg tggtggccac cgacagcaac tcggcacgtt
     6541 cgccggtggg gccgacctcg ttgccggagt cgttgaccgt gaagtcggcg cgaatgccct
     6601 cgcccaccat gcccggtttg cacgcggtga tgtagtcgcc gggttgggcg agcacgacga
     6661 gcttgcgggt gaggccggga ccgatgtttt cgacctcgcc gacgatccgc agaccgtcgc
     6721 tcgcgagcag gtagaactcg gtgacgtcgt cgccgtcgtt ggtcacctcg aacacgaggt
     6781 tgccgctggg agcctcggtg gccgaaagcg tgcactcggt cgcggtgctc gacacggcga
     6841 ccggcgcacc gccagcggtg ccatcggtgc catcggtgcc cgacgcggtg tcggggttgt
     6901 tgtcggtgca ggcggtgacg cccaacaggg cgacggccgc ggcaaaggcc cggtatcggt
     6961 ggttcatgtg gtcacctctg gttgaaagga ttgtggtgaa gtcggagccg aggagcctgt
     7021 cgtcggtgtt gcggggttgg ttctggtggt ctgcgacgga ccggtgctct ggcggatcgt
     7081 gcgcacgaac agcggaatca cgaccacgag atagacgctc cacgcgatgg cctcgagcca
     7141 ggtggtcgcc ggggagaagt tgatggtgcc cttgaggagc gtgccgtacc acgacgtcgg
     7201 cgggatctgg tcgctgacgt cgaacgcgag gttgttgagt ccggggagaa tcccggcctc
     7261 ctgcaggtcg tggatgccgt aactgaggat cccggcggcg acgatgatga gggcgaacgc
     7321 cgtccacgtg aagaacttcg acaggttgat gcgtacggcg ccgcgggtga tgaggaagcc
     7381 gagcacgatg gccgtggcga tgccgatgag ggcccccaac agcggttcgg tgttgttgcc
     7441 gctcgcctgg acgccggccc agacgaacag cgccgtttcg aggccttcgc gtccgaccgc
     7501 cagcatcgcc atgatggtga cggcggcggg cccgccctct gcggcgagcg acaggcgacc
     7561 ttcgaggtcg gtcttcaggg ttcgggcggt gcgcgccatc cagaagatca tccacgtgat
     7621 gaagccgacg gcgacgagcg acaggaagcc gccgatcgcc tcctgggccg tgaagctgag
     7681 ccctttcgga ccgaaggtga ggacggcgcc gaatccgagc gacacgacga cggcgatggc
     7741 gaccccggcc cacatcgggc ggagcagatg acggcggtcg gttcggacga ggtaggcgta
     7801 caggattccg atgaccaatg aggcctcgag tccttccctc agtccgatga gaaaattcga
     7861 catcatgtca agatcaccta acgatgagtg cggaggccac ctaacagccc tgccgacgtt
     7921 ttacacccat cgccgcgcga ggtgagaact cttgcccggt aaaaggtcac gtcgatggcc
     7981 acgtcgatgt cgaccgagcc gtggagcggc gcaagttgcc gagtgtttga cgaggctcga
     8041 cgtatcggga cgggaaacga cgattcggcg aggcgctagc gtcgtcgcca tggtctctgg
     8101 agcgcttgac ccgaacgttg cgaacccaca cggcgtttcg atgctgcgct tgggagacct
     8161 gacggccggg ccgatcgcga cggtggcgcc gggaggagtg gtgacgctgc ctggcgattc
     8221 gtccacccgt tggagctggt ggatcggggc cgaggatggt tggcatcgtc cggcccatga
     8281 gccgacgacc caggacgcgg cgctcgacgg cgctccgggc ctgcgcacga cggcgcgtat
     8341 tcccggcggt gacttcgtcg gcacggcgac cgccgtcaga ttcgacgcac tcgacggccc
     8401 gaccctgttg ttcgaactcg acaacgcctc ggcggtgccg gttgcggtgg cgttcgtcgt
     8461 cgagtccgac cgcccgatca cggtgcgcga cggcggcatc gatgtcgacg gggtcggcgc
     8521 gcttcgcctg acccgggtcg cggcggcctc gctcagcgca tcgtcggccg atgcgctggt
     8581 caccgcactg gtcgaggccg gcatcgccgc ggcgaccacg ggtg
//
LOCUS       DAOOMA010000030         8567 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_9670_length_8567_cov_3.825775, whole genome shotgun sequence.
ACCESSION   DAOOMA010000030 DAOOMA010000000
VERSION     DAOOMA010000030.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 8567)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8567)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8567
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9670_length_8567_cov_3.825775"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..334)
                     /locus_tag="PLP95_01685"
     CDS             complement(<1..334)
                     /locus_tag="PLP95_01685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HPB44542.1"
                     /translation="MSDTAAPEVYYDPYDFGIDDDPYPIWKALRDDHPLYYNEKFNFY
                     ALSRFRDVEAALKDWQTLSSAKGTLLELIKADIEMPPGTIIFEDPPNHTLHRGLLSRV
                     FTPRRMAAI"
     gene            complement(331..1782)
                     /locus_tag="PLP95_01690"
                     /pseudo
     CDS             complement(331..1782)
                     /locus_tag="PLP95_01690"
                     /inference="COORDINATES: protein motif:HMM:NF012398.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
     gene            complement(1903..3366)
                     /locus_tag="PLP95_01695"
     CDS             complement(1903..3366)
                     /locus_tag="PLP95_01695"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HPB44543.1"
                     /translation="MSYGGRLSELAGSDPDRVVARHVDPDGSPDHNFTLAELNTTAEA
                     CATEFASRGVSLDDLVAISLQNSPELLVSVFAAWKLGATPVPVRWDLPDWERSRVLAA
                     VSPALHVADGTAVSLAPQPFDTRSAPHRTALASEPVGAAAWGICSGGSTGTPKVIVAD
                     RPSRWAEAYGSCIMGHWREVARPQRVLVLAPMYHSTAFTSTLNLLAGDEIVVLRRFDA
                     ATALDTIERLDITNFVATPTMLARMADAQQRTPRDLSSLDWVLQGAAPLPPSLTRRWI
                     DLVGAQRFVMAYGMSEGLGLTAIRGDEWLEHPGSVGRGIRGTEVRVLDAERRPVEPGV
                     VGEIYLRTPGGSAQYLGDTPALPTTADGFSSAGDLGHLDADGYLHLADRRTDLIVTGG
                     ANVYPAEVESALIEHPAIADVVVVGLSDAQWGRRVHAIIEAAEGVEAPGSDDVVAFAR
                     SRLAAYKVPKTVEIVAAIPRSAATKVNRRALVEARGG"
     gene            complement(3446..4639)
                     /locus_tag="PLP95_01700"
     CDS             complement(3446..4639)
                     /locus_tag="PLP95_01700"
                     /inference="COORDINATES: protein motif:HMM:NF016777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HPB44544.1"
                     /translation="MPARDFPIFDADNHLYETEEAFTKFLPDRYKGAIDYVQVRGRTK
                     IVVRGQISEYIPNPTFEVVARPGAQEEYYRVGNPDGKSRREIYGEPMKAIPAFRAPAP
                     RLELMDEQGLDRTLMFPTLASLLEERMKDDVELTHAAIHALNEWLYETWQFDYEGRIF
                     TTPVITLPIVERAIEELEWVVERGARVVLIRPAPVPGLHGPRSFGLPEFDPFWEKVVE
                     HDVFVAFHSSDSGYSRFTGEWAGKQTEMLPFQPNAFRMMSTWRPVEDAAASLICHNTL
                     SRHPGLKVGFIENGSTWVRPLLENLADVYKKMPQEFMENPVEVFKRQVHISPFWEEDL
                     GKLADLIGPTQVLFGSDYPHPEGLEVPSTYIDDMAHVDAETMRLVMGGNLARLMRVDD
                     VAVAR"
     gene            4818..5585
                     /locus_tag="PLP95_01705"
     CDS             4818..5585
                     /locus_tag="PLP95_01705"
                     /inference="COORDINATES: protein motif:HMM:NF012596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HPB44545.1"
                     /translation="MSYDVSAVLQVDHDGPVRILRLNRPDHLNATNHELHEQLTKVFP
                     QIHEDFDARAVVLTGNGRAFSAGGDMAYIDEQIRDPDYRKLSLLHGKLIAAAMASCRV
                     PIIAAVNGPAVGLGCSLVAMSDIVYMARSAYLADPHVSVGLVAADGGAISWPLMMSLH
                     LAKEYALTGDRITAERAERIGLANHVVADDEVLDAALACAHRIAKQPRGAVEDTRRML
                     NHHLDRALNGSIDFALTAEFRSFDSPELRATVDRFLD"
     gene            complement(5663..7144)
                     /locus_tag="PLP95_01710"
     CDS             complement(5663..7144)
                     /locus_tag="PLP95_01710"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0102662 - malonate-semialdehyde
                     dehydrogenase (acetylating, NAD+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-acylating methylmalonate-semialdehyde
                     dehydrogenase"
                     /protein_id="HPB44546.1"
                     /translation="MERIDHWIGGKLLSRDGRVSPVFNPATGVQRAEVSLADSALVSE
                     AVGVAAGAFEDWRYSTLSKRAKLMFNLRELLTRNADDLARIVVGEHGKVLDDARGEVE
                     RGLECIEFACGIPSLLKGGRNPEVSSGIDVHTAYEPVGVIAAITPFNFPVMVPLWMLA
                     NAIACGNTVVLKPSERDPSASMRIAELVTEAGFPDGVVNVLHGDATAVNELLASPDVA
                     AVSFVGSTPVARSIYEVGAKHAKRVQALGGAKNHMVVLPDADVDAAADAAISAGYGAA
                     GERCMAISVIVAVGAVADPLVEAIVKRIPDVKIGSGLDSSSMMGPLITEAHRDRVRGY
                     VDGAPAEGATIVVDGTDGIPADGFYHGCALLDHVTPGMKAYDDEIFGPVLSVVRVDTF
                     DEALTLVNDNPYGNGTAVFTRDGGAAREFERKVQAGMVGINVPIPVPVAWHSFGGWKA
                     SIFGDSAIYGPDGIRFYTRPKVVTTRWPDPASSAIDLGFPTTD"
     gene            7273..7770
                     /locus_tag="PLP95_01715"
     CDS             7273..7770
                     /locus_tag="PLP95_01715"
                     /inference="COORDINATES: protein motif:HMM:NF016777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HPB44547.1"
                     /translation="MDRTTVNGLGLAYEVIGERGRPWIITPGGRFSKDYGGKMPSEVF
                     RDHILACYITDPSGLLLRDRIGIDNIAWECDYPHTDTTWPNSPEFTWGELQDAGCRDD
                     EIHKITWENACRFFDWDPFKHTAREDATVGALRARADAAGVDVTRMPKAQWRERNEAA
                     GIGVF"
     gene            7778..>8567
                     /locus_tag="PLP95_01720"
     CDS             7778..>8567
                     /locus_tag="PLP95_01720"
                     /inference="COORDINATES: protein motif:HMM:NF004846.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HPB44548.1"
                     /translation="MSTTNTWTTAEIADQRGRVFVVTGANSGLGFETARTLARTGATV
                     VMACRSPERAATALDDLRNTVPDADVEVQQLDLASLESVRTAAAALRQRFGAIDALIN
                     NAGVMYTERELTRDGIELQFGTNHLGHFALTLELIDSLEAAPRARVVTIGSLGHRLPY
                     AFDLDDLTATGKYSRFGAYARSKVANLLFSYELDRRLQAAGSTVISLAAHPGASDTEI
                     AEHLPGMKWQRKYLHRLAQPASLGCLPAVRAATDLEAVGGQYYGP"
BASE COUNT         1472 a         2905 c         2747 g         1443 t
ORIGIN      
        1 cgatcgcggc catgcgacgc ggcgtgaaga cccgcgacaa cagcccgcga tgcaacgtgt
       61 ggttcggcgg atcctcgaag atgatcgtgc cgggcggcat ctcgatgtcg gccttgatca
      121 actcgagcag ggtccccttg gccgaactca gcgtctgcca gtccttgagc gccgcctcga
      181 cgtcgcggaa ccggctcagc gcgtagaagt tgaacttctc gttgtaatag agcggatggt
      241 cgtcgcgcag cgccttccag atcggatacg ggtcgtcgtc gatgccaaag tcgtaggggt
      301 cgtaatagac ctcgggtgcg gccgtgtcgc tcatgaggcc ggccaagcgt aggacttgag
      361 ttcgagatac tgcgcgaacc cggcgtcacc gttctggcgg ccgaccccgg agtatttgaa
      421 cccgccaaag ggcaggtccg ctccgtacgg ggcaccgccg ttgatgctca ccatgccggt
      481 gcgcagccga tccgccaccg ccttggcccg ctcgaagctg cccgccatca ccccaccgcc
      541 gagtccataa cggctgtcgt tggcaatgcg caccgcatcg tcttcgtcct cataaggggt
      601 cacgacgagc accggaccga agatctcctc ctgtgcgatc gtcatcgaat tgtcgacgtc
      661 gacgaacagg gtcggctcga cgaagaatcc gttgtcgaac tcggggccgg tcgggatgcc
      721 gccgcccagc accaacttgg cgccttcctc ctggcctctt tggatgtagc ccatcacccg
      781 gtcgcgctgt ttcgccgaga tctgcgggcc ctgcaacaca tcgggccgct gcgggtcgcc
      841 atagggcacg ttcttcatca tgtcgatgag cgcgttgacc ccctcgtcgt aatgcgactt
      901 cggcaacagc atgcgggtcg ggatcgcaca cccctgcccg gcgtgggtac acacggcgag
      961 gcccatgaac gaggcggcga gcagatcggc atcgtccaac accaccgtcg ccgactttcc
     1021 accgagttcg aggaagagcc gcttcatggt ccgcgagcct ctttccatga tcccctgccc
     1081 gaccgcggtg gagccggtga acgagatcat gtcgaccagc ggcgacagcg tgagttgctc
     1141 gccgcgaagg tgatccgagg aggtcacgat attgaccaca cccgcaggaa tgtcggtgcg
     1201 ctcggcgata agccgtccga gcagcgtcgc gttcagcggc gtgtccgggg cgggcttcag
     1261 caccacggtg ttgccggtgg ccagtgcgta ggcgagcttg atgatcgcga cctcgaaggg
     1321 gaagttccag ggggtgatgg cgccgacgac accggcggcc atcgacacga cgctgcggcg
     1381 atcgagggaa ccctagatgc tgatcgcctc gccgaggtcg cgctcccatt cccaggtgct
     1441 gatcatcgag atgggatggc gcaacgagtg gttcagcgga acgtcgagtt gcgcgctgtg
     1501 ggtcaacatc cgcggcgcgc ccacttcctg gatgagcaac tcgcgaagct cctcgcactc
     1561 ggcatcgagc gcgtcctgga actgattgag gcagtggatg cggaactcgt gattcgtcga
     1621 ccagtcggtc tcgtcgaagg cgcgccgggc tgcggcgagg gcggcctcca tgtcctgcgc
     1681 ggttccgtcg gccaccttgc cgacgagccc acccgtggcg ggattgaaca cgtcgaaggt
     1741 ggacccgtta ctggcgtcga cgagcttgcc gtcgatcaac attcgggatt cgggatggat
     1801 ggttggcagg tcggtcatgg gttcccctgg gtattcggtc gataggtgaa gggttgagcg
     1861 gtcgatatgt gtcggtggga cggcggtggg tcgtggctgg gtctacccac cgcgagcctc
     1921 caccagtgct cggcggttga ccttcgttgc ggcgctgcga ggaatcgccg cgacgatctc
     1981 gacggtcttg ggcaccttgt aggcggcgag gcgcgacctg gcgaaggcca cgacatcgtc
     2041 ggaccccggc gcctcgactc cctcggccgc ttcgatgatc gcgtgcacgc gtcgacccca
     2101 ttgcgcatcg ctcagcccga ccaccacgac atcggcgatc gccgggtgtt cgatcaacgc
     2161 agattcgact tcggccggat acacgttggc gccgccggtc acgatgaggt cggtgcgacg
     2221 gtcggcaagg tggaggtacc cgtcggcatc gaggtgaccg aggtcgccag cggatgagaa
     2281 cccgtccgcg gtggtcggta gcgccggggt gtcgccgaga tactgcgccg aaccgcccgg
     2341 ggttcgcagg tagatctcgc cgacgacccc cggctcgacc gggcgtcgtt cggcgtcgag
     2401 cacccgcact tcggtgccgc gaatcccgcg accgacactg cccggatgct cgagccattc
     2461 gtcgccacga atcgccgtga ggcccaggcc ctcgctcatg ccgtaggcca tcacgaagcg
     2521 ttgcgcaccg acgaggtcga tccagcgccg ggtgaggctc ggcggcagcg gggcggcgcc
     2581 ttgaaggacc cagtcgaggc tcgacaggtc gcgtggggtg cgctgttgag cgtcggccat
     2641 gcgggcgagc atggtcggag tggccacgaa gttggtgatg tccaggcgtt cgatcgtgtc
     2701 gagcgccgtc gctgcgtcga agcggcgcag caccacgatc tcgtcgccgg ccaagaggtt
     2761 gagggtcgag gtgaaggcgg tcgagtgata catgggcgcc agcaccaaca cccgctgcgg
     2821 ccgcgccacc tcgcgccagt gccccatgat gcacgagccg taggcctcgg cccatcggct
     2881 gggccgatcg gccacgatga ccttcggggt gcccgtcgaa ccgccgctgc agatccccca
     2941 cgccgcggcg ccgacgggtt cgctggccaa cgcagtgcga tgtggcgccg accgcgtgtc
     3001 gaagggctgt ggagcgaggg acacggccgt gccgtcggcg acgtgcaacg ccggcgacac
     3061 ggccgcgagc acgcgggacc gttcccaatc gggcaggtcc caccgaaccg gaaccggagt
     3121 cgctcccagc ttccacgccg cgaagaccga aacgagcagt tcgggtgagt tctgaagcga
     3181 gatggcgacc agatcgtcga ggctgactcc ccggctggcg aactccgttg cgcatgcctc
     3241 ggcggtcgtg ttcagttcgg cgagcgtgaa gttgtgatcc ggcgagccgt cggggtcgac
     3301 atgacgcgcg acgacgcggt cgggatcact cccggccagt tcgctgagtc ggcccccgta
     3361 actcatcgcc tcgctgtcgt cccgtctcgt ctcgtcgtgc cctgcgtcgt acccgtgtcg
     3421 tgccctgcgt gcctcggccg gacccctacc gggccaccgc gacgtcgtcg acccgcatga
     3481 gccgagcgag gttgccgccc atgaccagac gcatcgtctc cgcgtcgaca tgtgccatgt
     3541 cgtcgatgta ggtactcggg acttccagcc cttcggggtg cggatagtcg gaaccgaaca
     3601 gcacctgggt cgggccgatg agatccgcga gtttgccgag atcctcctcc cagaacgggc
     3661 tgatgtgcac ctgccgcttg aagacctcga ccgggttctc catgaactcc tggggcatct
     3721 tcttgtagac gtcggcaagg ttctcaagca gcggccgcac ccaggtgctg ccgttctcga
     3781 tgaatccgac cttgaggccc gggtggcgag agagcgtgtt gtgacagatg agcgacgccg
     3841 ccgcatcctc gacgggccgc cacgtcgaca tcatgcggaa ggcattcggc tggaacggca
     3901 acatttcggt ctgcttgccc gcccattccc cggtgaagcg cgagtagccg ctgtcggagg
     3961 agtggaaggc cacgaacaca tcgtgttcca ccaccttttc ccagaacggg tcgaactcgg
     4021 gcagcccgaa ggaccgcggg ccatgcaggc cgggcacggg ggcgggacga atcaacacga
     4081 cgcgcgcacc gcgctcgacg acccactcga gttcctcgat cgcccgttcc acgatcggca
     4141 gagtgatcac cggcgtggtg aagatgcgtc cctcgtagtc gaactgccag gtctcataca
     4201 gccactcgtt gagcgcgtga atcgccgcgt gggtgagttc gacgtcatct ttcatccgct
     4261 cctcgagcag gctggcgagc gtcgggaaca tcaaggtgcg atcgaggccc tgttcgtcca
     4321 tcaactccag gcgcggggca ggtgcacgaa acgccgggat cgccttcatc ggctcgccgt
     4381 aaatctcgcg gcgcgacttc ccgtcgggat ttccgacccg gtagtactcc tcttgagcgc
     4441 cgggccgggc gacgacctcg aaggtcgggt tggggatgta ctcactgatc tggccgcgca
     4501 cgacgatctt ggtgcggccg cgaacctgca cgtagtcgat ggcacccttg taccgatccg
     4561 gcaggaactt cgtgaacgct tcctcggtct cgtacaggtg gttgtcggca tcaaagatgg
     4621 gaaagtctcg tgcgggcatg ttgtccccct cctcgtcgat ccgcgatacc ccaaccccgg
     4681 atcgatggca tgtcatcggc gttccgcacg ctacatgtaa gcgaacgctc acttcaatag
     4741 ggtacggttg cgcatctgat caaccttcaa aatcgacttt gacgccactg tcaaaagtgg
     4801 gtcgcaaagg actgtacatg agttacgacg tgtccgcggt actccaggtc gaccacgacg
     4861 gaccggtgcg aatcttgcgc ctgaaccggc cagaccacct caacgccacc aaccacgaac
     4921 tgcacgaaca gctcaccaag gtgttcccgc agatacatga ggacttcgac gcccgcgccg
     4981 tcgtactcac cggtaatgga cgggcgttct ccgccggtgg cgacatggcc tatatcgacg
     5041 aacagatccg cgaccccgac taccgcaagt tgtcgctgct gcacggcaag ctcatcgccg
     5101 ccgcgatggc ctcgtgtcgc gtgccgatca tcgccgcggt caacgggccc gccgtcgggc
     5161 tgggctgctc actggtcgcc atgtccgaca tcgtctacat ggcgcgcagc gcctatctcg
     5221 ccgacccgca cgtgagcgtg ggcctcgtcg cggccgacgg cggtgccatc tcgtggccgc
     5281 tcatgatgag cctgcacctg gccaaggagt acgcactcac gggcgatcgg atcacggccg
     5341 aacgagccga acgcatcggg ctcgccaacc acgtggtggc cgacgacgag gtcctcgacg
     5401 ccgcgctcgc gtgcgcacac cgcatcgcta aacaacctcg gggtgcggtc gaggacaccc
     5461 ggcgcatgct caaccatcac ctcgaccggg cactcaacgg atcgatcgat ttcgccctga
     5521 ccgcggagtt ccgctccttc gactcgcccg aactacgcgc caccgtcgac cgcttcctcg
     5581 actgacccct ccggccacgc cgtcacccag actcgcggca aacccccgcc gggggaacgc
     5641 ggcgggttgg ccagttggcg gctcagtcgg tggtcgggaa gccgaggtcg atcgcgctcg
     5701 acgccgggtc cggccaacgc gtggtgacga ccttgggtcg ggtgtagaag cggatgccgt
     5761 cggggccgta gatcgcgctg tcgccgaaga tcgacgcctt ccatccgccg aagctgtgcc
     5821 aggcgaccgg cacggggatc ggcacgttga tgccgaccat gccggcttgc accttgcgtt
     5881 cgaactcgcg agccgctccc ccgtcgcggg tgaacaccgc cgtgccgttg ccatacgggt
     5941 tatcgttgac gagcgtgagc gcctcatcga aggtgtcgac gcgcaccacg ctgagaacgg
     6001 ggccgaagat ctcgtcgtca taggccttca tcccgggcgt cacgtgatcg agcaatgcac
     6061 acccgtgata gaagccatcc gcggggatgc cgtcggttcc gtcgaccacg atcgtggccc
     6121 cctccgccgg tgcgccgtcg acgtatccgc gcacccggtc gcgatgcgcc tcggtgatga
     6181 gcggccccat catcgacgac gagtcgagac ccgacccgat cttcacgtcg gggatgcgct
     6241 tgacgatggc ctccaccagc ggatcggcga cggcaccgac cgccacgatc accgagatcg
     6301 ccatgcagcg ttcgcccgcc gcgccgtacc cggcggaaat tgctgcatcc gccgccgcgt
     6361 cgacatcggc atcgggcaac acgaccatgt ggttcttcgc cccgccgagt gcctgaaccc
     6421 gcttggcgtg cttggccccg acctcgtaga tcgagcgcgc caccggggtg ctgccgacga
     6481 aggacaccgc ggcgacatcg ggcgatgcga gcagttcatt caccgccgtg gcgtctccgt
     6541 gcaagacgtt gaccaccccg tcggggaacc cggcctcggt caccaactcg gcgatgcgca
     6601 tcgacgcgga agggtctcgc tcggagggct tcaacaccac cgtgtttcca caggcgatgg
     6661 cgttggccag catccacagc ggcaccatga ccgggaagtt gaacggggtg atcgccgcga
     6721 tgacaccgac cggctcgtat gcggtgtgga cgtcgatacc gctcgacacc tccgggttgc
     6781 gcccgccctt gagcaacgaa ggaatcccac acgcgaactc gatgcattcg aggccccgtt
     6841 cgacctcgcc tcgggcatcg tcgagcacct tgccgtgctc gcccacgacg attcgggcga
     6901 ggtcatccgc attgcgggtc aggagttcgc gaaggttgaa catcagcttc gcccgcttcg
     6961 acagcgtgga atagcgccaa tcctcaaagg ctcccgcggc gacgcccacc gcctcggaca
     7021 ccaaggccga atcggcgagc gacacctcgg cgcgctgaac cccggtcgcc gggttgaaca
     7081 cgggtgacac tcggccgtcc cggctcaaca acttgccccc gatccaatgg tcgatgcgct
     7141 ccatcggctc tcctctgctc tgcagactcc gctctgcgga cactgctctg tgaactctgc
     7201 tctgtggact ctacgaacag agggagtaag taaacgctcc ctcacttgta tctgcacgct
     7261 accctcgccc acatggaccg cacaacggtg aacggcctgg ggttggccta cgaggtgatc
     7321 ggcgagcgag gacgcccgtg gatcatcacc ccgggcggac gtttcagcaa agactacggc
     7381 ggcaagatgc cctcggaggt gtttcgcgat cacatcctgg cctgctacat caccgaccct
     7441 tcggggctgt tgctgcgcga ccggatcggc atcgacaaca tcgcctggga gtgcgactac
     7501 ccccacaccg acaccacctg gccgaactca ccggagttca cgtggggcga actgcaagac
     7561 gccggttgcc gcgacgacga gatccacaag atcacctggg agaacgcctg ccggttcttc
     7621 gactgggacc ccttcaaaca caccgctcgc gaagacgcca ccgtcggggc gcttcgggca
     7681 cgcgccgatg ccgccggcgt cgacgtgacc cgcatgccca aggcgcagtg gcgcgagcgc
     7741 aacgaggccg ccggcatcgg cgtgttctga ttcgcagatg tccactacca acacctggac
     7801 caccgcggag atcgccgacc aacgcggccg ggtcttcgtc gtgaccggcg cgaactcggg
     7861 cctcgggttc gaaaccgccc gcacactcgc tcgcaccggc gccacggtgg tgatggcctg
     7921 ccgcagcccc gaacgcgccg cgacggcact cgacgacctc cgcaacacgg tgcccgacgc
     7981 cgacgtcgag gtacaacaac tcgacctggc gtcactcgaa tcggtgcgca ccgcagccgc
     8041 cgcgctgcgc cagcgattcg gggcgatcga cgccctcatc aacaacgccg gggtgatgta
     8101 caccgaacgc gaactgaccc gtgacggcat cgagttgcag ttcggcacca atcacctcgg
     8161 acacttcgca ctcaccctcg agttgatcga ctcgttggaa gccgccccgc gggcacgggt
     8221 ggtcaccatc ggcagtctcg ggcaccgact tccgtacgcg ttcgacctcg acgatctgac
     8281 cgcaaccggc aagtacagcc gctttggcgc ctacgcgcgt tcgaaggtcg cgaacctgtt
     8341 gttctcctac gagctggatc gacgtctgca ggcggctgga tcgacggtca tctcgttggc
     8401 ggcccatccc ggtgcgtccg ataccgagat tgccgagcat ctcccgggga tgaagtggca
     8461 gcgcaagtac ctgcaccggc tcgcgcaacc ggcctcgctc ggatgccttc cggccgtgcg
     8521 cgccgccacc gacctggagg ccgtcggcgg gcagtactac gggccag
//
LOCUS       DAOOMA010000031         8555 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_9688_length_8555_cov_4.509176, whole genome shotgun sequence.
ACCESSION   DAOOMA010000031 DAOOMA010000000
VERSION     DAOOMA010000031.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 8555)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8555)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8555
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9688_length_8555_cov_4.509176"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..348
                     /locus_tag="PLP95_01725"
     CDS             <1..348
                     /locus_tag="PLP95_01725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HPB44549.1"
                     /translation="NGVLPDDPVLHQCVMAYASDITLLDTSLKPHGGSGLGDVFMASL
                     DHAMWFHRPFRADEWLLYHQDTPTAQGGRGLGRGLIFSSDGTLVASVMQEGVIRPARV
                     PVLRPDADGRSQR"
     gene            509..1627
                     /locus_tag="PLP95_01730"
     CDS             509..1627
                     /locus_tag="PLP95_01730"
                     /inference="COORDINATES: protein motif:HMM:NF019607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HPB44550.1"
                     /translation="MTSTSAPATSAAPLATARVTLTEVADVAEAIALARRFGDDDAMY
                     VASRDGRIERIPLDGSDPSMLLDLRDRVGDYQGEQGLLGIAWNLDGTVLIVSYTDGDD
                     GGASVIERYPFEAGAQSPSADPTELLRVAQPATNHNGGHVEFGPDSMLYIGFGDGGGQ
                     GDPGNRAQDLTSLLGKILRIDVAAPTYTIPADNPYVDGPGGARREIWIAGVRNPWRFS
                     FDQATGDLWIADVGANSWEEVNVLRAADGLGRGANLGWDRREGTHPTDEDDGSHPDGG
                     FHEPILDYGHDQGASITGGVVVRDPRLGELDGVYLHSDFAAAKLWGLRVDGDAVVDSG
                     EIDTNDTTMNSVVSFGVGPNDEVYIASLSGTVWRVDPA"
     gene            complement(1656..2114)
                     /locus_tag="PLP95_01735"
     CDS             complement(1656..2114)
                     /locus_tag="PLP95_01735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012224976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HPB44551.1"
                     /translation="MGTDATVTVRELSAADVDASIAEAFAGLLPQLSSSSPRPTVEQL
                     ARIAGSDCNHLLIAESEDSTVLGSMTLVVFEIPTGVRAWIEDVVVDESARGRGVGEAL
                     NRRAIDIAHEVGAKSVDLTSRPSREAANRLYQKLGFVARETNVYRYQGSR"
     gene            2188..3027
                     /locus_tag="PLP95_01740"
     CDS             2188..3027
                     /locus_tag="PLP95_01740"
                     /inference="COORDINATES: protein motif:HMM:NF021287.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043248 - proteasome assembly [Evidence
                     IEA];
                     GO_process: GO:0051131 - chaperone-mediated protein
                     complex assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAC2 family protein"
                     /protein_id="HPB44552.1"
                     /translation="MTYLRWLDRPAVKEPIVIASFEGWNDAGEAASQTVRLLERWLDA
                     TPFVDIDPEEFFDFTVVRPHIGSDGDGIRRIDWPENVFRVATPEGSPPIVLLCGIEPQ
                     LRWRAFSHCVLEVAQHLDARLVLTLGSLLAEVPHTRPSQVFGAAGNVEVSRILDYEPS
                     RYEGPTGISGVLHELCATRGTHSASLWATVPSYVPSAPSPKATRALAVEVSRILGIDL
                     PIEELDDDVEDYDRQITDMVSEDDDTVTYVAHLEERYDREALAGHSADALVSEVEAFL
                     RDQ"
     gene            complement(3059..5365)
                     /locus_tag="PLP95_01745"
     CDS             complement(3059..5365)
                     /locus_tag="PLP95_01745"
                     /EC_number="2.7.7.59"
                     /inference="COORDINATES: protein motif:HMM:NF002895.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008773 - [protein-PII]
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[protein-PII] uridylyltransferase"
                     /protein_id="HPB44553.1"
                     /translation="MKVETELAALLQRSDLLGRAFTTDYTAVVERWLCSLYEDLVGLD
                     DGVALVATGGQGRGELSPFSDLDLLLLHDGSLDGSTAQSLWYPLWDAKMKVGHAVRTP
                     AETLQLAATDLATATGLLSARHLAGDASLTAALVEAHLVEWKRRAKRAFGELVDTATR
                     RHADASSVANALEPDLKEGRGGLRDVHLLGWIEAADQGLDIASADDLAEDYDLVLAVR
                     VELHRLSNRPGDVIRLDDQAEVASRLGFADAMELMSELAGSARRIAWAADDACSRLQG
                     WLRPSSRLRPRRSPSLEGPYELDGGFVVVAPDAPSSVDAIEPLLVALAAARHGARIHR
                     GALERISTAAAAEEPWPDELRRRFVELLACGPAAIEAIESLDLVDSWVALIPEWAPLR
                     CRSQRNAYHRFAVDRHSWECVAMAADFFDRVDRGDLLLLAALFHDIGKGYPGDHSVVG
                     VQVARPALRRMGLSEADVRTVDVLIEHHLLLADVATRRDLDDPLTILSVAARVETVQR
                     LGLLEALTTADSLATGSTAWSPWKAGLVAELVRKVGHVLGGGAVEDVTGAATLSADRR
                     RLIEGRDPNAEPVVEADGNRVTIVCIDRPGVFSRITGVLALNGLDVLEATVQSDDGWA
                     VEEFVVESTFASEIPWAKVARDIDRALRSRFALEARLAERRRSYRRAVRTVGTLEPKV
                     RVVRGEASDGATVVEVIGRDRIGLLHQLTRVLSDLDVDIVRAKIATLGGDVVDTFYVR
                     GRDDLPIDDDDLVEIKSALLFELRRPDA"
     gene            complement(5489..6025)
                     /locus_tag="PLP95_01750"
     CDS             complement(5489..6025)
                     /locus_tag="PLP95_01750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB44554.1"
                     /translation="MSQPSSDRPAEPDHANPNGNTAPFDTDLAAMRELSDRLIAVATV
                     GVHRWVLDVVAERISGLDATRAAAVLDAARGAADEAATDVHVRLAELLAVPVDEQSTT
                     PLAILRGAHRYPTAVLSEAGAIPVQRDPFEERVNPDDLFGVAPGSWADFGEEVLDAGM
                     AWGAAKAFLHKRARRQAP"
     gene            complement(6035..7213)
                     /locus_tag="PLP95_01755"
     CDS             complement(6035..7213)
                     /locus_tag="PLP95_01755"
                     /inference="COORDINATES: protein motif:HMM:NF023223.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2220 family protein"
                     /protein_id="HPB44555.1"
                     /translation="MVATLNARWQRGRYLREALLGEPFEPVRVSLGGPKAADLSQHFS
                     EVIDWADAVHSAAAPHGRVETRTMKTRSIGENEIPVAVWFDSIDQLIAALGVRRELQQ
                     AEQLVAATGAALGDEAARWVAQHPHPALGSQPWWPGIIAVTQWIAATDVSGLDLRHVD
                     VGGAGAQYGIDTKFITAHGAIIGSLLDVVLPPERVNPIESEFVARYGFRRPPRYVRFR
                     LLDEVEELPDGISELTLRTDELAAMPLSVEQVFIVENQATYLAFPPVPRSIVIFGGGF
                     GATQLGDIAWLAERRVLYWGDLDLAGFAILDAVRQRVGNVESMLMDRATLSSHLAHVV
                     NDAKAKPAMLTHLTAEESATYRDLVEDRYGASLRLEQERIRFSHVRAALSELRRGDID
                     "
     gene            complement(7288..>8555)
                     /locus_tag="PLP95_01760"
     CDS             complement(7288..>8555)
                     /locus_tag="PLP95_01760"
                     /inference="COORDINATES: protein motif:HMM:NF024947.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SbcC/MukB-like Walker B domain-containing
                     protein"
                     /protein_id="HPB44556.1"
                     /translation="ASVELQALTAQLDAVVARIVEESTERDSVKTEIGRVTGEAERTE
                     AQRNDLQQLLGLVEAEVARGYFDELEEHLSIEHRRGALQLDGIDSARSGATEGLRSAQ
                     HRATERAHGAERQAERAMAGFRARFQRETAELDASVAAGPEYREMYERVRTDDLPRFE
                     AEFRTSLRENTIKEIAQLSADLEGRQRDIRRHIDTINGSLHEIDYNPGRFIRLLPEAT
                     PNTEIRGFRDELRAVTSRTTNPGDDDYSEHRFLQVKALIDRFVGREGSTDQDRAWTKR
                     VTDVRNWFVFSASERWRSDDTEYENYADSSGKSGGQKEKLAYTVLAASLAYQFSHGST
                     AGAGSFRFVAIDEAFGRGSDESTRFGLRLFRTLGLQLLIVTPLQKIRVIEPFVSTVGY
                     VVNPEENNSQLVQMTMSEHHRRRESAGQA"
BASE COUNT         1426 a         2916 c         2772 g         1441 t
ORIGIN      
        1 aacggcgtac ttccggacga ccccgtgctc caccagtgcg tgatggccta tgcctccgac
       61 atcacgttgc tcgacacctc gttgaaaccc cacggcggca gcgggttggg cgatgtgttc
      121 atggccagcc tcgatcacgc catgtggttc caccgcccgt ttcgcgccga tgagtggctg
      181 ttgtatcacc aggacacccc aacagcgcag ggcgggcggg ggctcggccg tgggctgatc
      241 ttcagtagtg acggaaccct ggtcgcgtcg gtgatgcagg agggggttat tcgtccggct
      301 cgcgtgccgg tgctgaggcc cgacgccgac gggcgttcgc agcggtgagg tggtgatgcg
      361 ccaccgccct acgccaccca cccgccggtt ggcggccgtg ctcggggtct cggcgttggc
      421 gacgctgggc gcgtgcggag acggaggtgg ttccgacacg ccggacacgg ctcccgtggc
      481 ttccgctgtc acctcgacga cagtctccgt gacctcgacc tccgcaccgg cgacgtcggc
      541 cgcaccgctc gcgacggccc gggtcaccct caccgaagtt gccgacgtgg ccgaggcgat
      601 cgccctcgct cgacgcttcg gcgacgatga cgcgatgtac gtcgcgtccc gcgacgggcg
      661 aatcgaacgg ataccgctcg atggctcgga cccgtcgatg ttgctcgacc tgcgcgaccg
      721 cgtcggcgac tatcagggcg agcaggggtt gttggggatc gcttggaacc tcgacggtac
      781 cgtgttgatc gtgtcctaca ccgacggcga cgacggcggt gcgagcgtga tcgagcgcta
      841 ccccttcgaa gcgggtgccc aaagcccatc ggccgaccca accgagttgc ttcgcgtcgc
      901 tcaacccgcc acgaaccaca acggcggcca cgtcgagttc ggaccggact cgatgctgta
      961 catcggattc ggcgacggcg gaggtcaggg cgatccgggc aaccgcgctc aagacctgac
     1021 gtcgttgctc ggaaagattc tgcggatcga cgtcgccgca ccgacctaca ccattcccgc
     1081 cgacaacccc tatgtcgacg gccccggagg ggcgcgtcgt gagatctgga tcgctggggt
     1141 gagaaacccc tggcgcttct cgttcgacca ggcgaccggt gacctgtgga tcgccgatgt
     1201 cggcgcgaac tcgtgggagg aagtcaacgt gttgcgagcc gccgacggct tgggacgcgg
     1261 cgcgaatctc ggttgggacc ggcgcgaggg cactcacccg accgacgagg acgacgggtc
     1321 gcaccccgac ggcggatttc atgagccgat cctcgactac ggccacgacc agggagcgtc
     1381 gatcaccggc ggcgtcgtcg tgcgcgatcc ccgcctcggc gaacttgacg gggtctacct
     1441 ccattccgat ttcgccgcag cgaagctgtg gggcctacgc gtcgacggtg acgcggtcgt
     1501 cgattccgga gagatcgaca cgaacgacac gacgatgaac tcggtcgtca gctttggcgt
     1561 cggcccgaat gacgaggtgt acatcgcctc actcagcggc acggtctggc gggtcgaccc
     1621 ggcctgagct ggccggggtc gacacccgac ctcgcctatc gcgaaccctg atagcgatag
     1681 acgttcgtct cccgggcgac gaaacccagc ttctgataca ggcgattcgc cgcctcgcga
     1741 ctcggccgcg aggtcaggtc caccgatttc gctccaacct cgtgggcaat gtcgatcgca
     1801 cgccggttga gggcttctcc cacgccacgg ccgcgagccg actcgtcgac caccacgtcc
     1861 tcgatccagg cgcggacccc ggtggggatc tcgaagacca ccaaggtcat cgagccgagc
     1921 acggtggaat cctccgattc cgcgatgagc aggtgattac agtccgagcc ggcaatccgg
     1981 gcgagttgtt cgaccgtcgg gcgcggcgac gacgaactca actgcgggag caggccggcg
     2041 aaggcctcgg cgatggaggc gtcgacatcg gcggcggaga gttcgcgaac ggtcacggtg
     2101 gcatcggttc ccatggctcc agcgtacggg atcgcgtttc ggttccagac ggagggaact
     2161 gacgtgttcg tcgatagcgt tttgtgggtg acctatcttc gatggctcga ccgaccggcg
     2221 gtcaaagaac cgatcgtcat cgcgtcgttc gagggttgga acgacgccgg tgaggccgca
     2281 tcacagaccg ttcgtctgtt ggagcgctgg ctcgatgcga cccccttcgt cgacatcgac
     2341 cccgaggagt tcttcgactt caccgtcgtt cggccccaca tcggaagcga tggggacggt
     2401 attcggcgca tcgactggcc cgagaacgtc tttcgtgtgg cgacgcccga aggttcaccg
     2461 ccgatcgtgc tgctgtgtgg aatcgaaccc cagcttcgtt ggcgagcgtt ttcgcactgc
     2521 gtgctcgaag tggcacaaca cctcgacgcc cgactcgtgt tgacgctcgg aagcctcctc
     2581 gcggaggttc cccacactcg cccctctcag gtcttcgggg ccgccggcaa cgtcgaggtg
     2641 tcgcggattc tcgactacga gccgtcccgg tatgaggggc ccaccggaat ctcaggcgtg
     2701 cttcacgaac tgtgcgcaac acgtggcacc cacagcgcgt cgttgtgggc gacggtaccc
     2761 agttacgttc cgtcggcccc gtcacccaag gcgacccgag ccctcgcggt cgaggtcagc
     2821 cgaattctgg ggatcgacct tcccatcgaa gaactcgacg acgacgtcga ggactatgac
     2881 cgccagatca ccgacatggt gtccgaggac gacgacacgg tcacctatgt cgcccatctc
     2941 gaagagcgtt acgaccgcga agcactcgcc ggacacagcg ccgacgcgtt ggtcagcgag
     3001 gtcgaggcgt tcctacgcga ccagtagtag cgaccggcat cggcgttcgc cgcccggctc
     3061 aggcatcggg gcgacgcagt tcgaacagca gcgccgactt gatctcgacg aggtcatcgt
     3121 cgtcgatggg caggtcgtcg cgaccgcgga catagaacgt gtcgaccaca tcgccgccga
     3181 gggtggcgat cttggctcgg acgatgtcga catcgagatc cgacagcacg cgcgtcagct
     3241 ggtgcaggag accgattcgg tcccgtccga tgacctcgac cacggtggcg ccatccgacg
     3301 cctcccctcg cacgacacgg accttcggct ccagggtccc aacggttcga acggcacggc
     3361 ggtagctgcg tcggcgttct gccaggcgcg cctcgagtgc gaatctcgag cgcaacgccc
     3421 ggtcgatgtc gcgggcaacc ttcgcccacg gaatctccga agcgaatgtc gactcgacga
     3481 caaactcctc gacggcccag ccgtcgtcgg actgcaccgt cgcctcgaga acatccagtc
     3541 cgttgagcgc gagcaccccg gtgatgcggc tgaacactcc gggacggtcg atgcacacga
     3601 tggtgacccg attcccgtcc gcctcgacca ccggctcggc gttcgggtcg cgcccttcga
     3661 tgaggcgccg ccgatcggcg ctcagcgtcg ccgctccggt cacgtcctcg actgcgcccc
     3721 cgccgagcac gtgaccgacc tttcgcacca gctcggcgac gagaccggcc ttccacgggc
     3781 tccaggcggt ggatccggtc gccaacgagt ccgcggtggt cagggcttcg aggaggccga
     3841 ggcgttgcac cgtctcgact cgcgcggcga cggacaggat cgtcagcgga tcatcgaggt
     3901 ctcgacgcgt cgcgacgtcc gcgagcagca ggtgatgttc gatcagcacg tcgacggttc
     3961 gcacgtcggc ttcggagagc cccatgcgac gcaaggccgg tctggcaacc tgcaccccga
     4021 cgacgctgtg atcgcccggg taccccttgc caatgtcgtg aaagagcgcc gccaacaaca
     4081 gcaggtcacc gcgatcgacc cgatcgaaga agtccgcggc catggcgacg cattcccacg
     4141 aatgccgatc gaccgcgaac cggtgatagg cgtttcgctg cgatcgacat cgcaacggtg
     4201 cccactcggg gatcagcgca acccaactgt cgacgaggtc gagcgactcg atcgcctcga
     4261 tcgctgctgg tccgcacgcc aacaactcga cgaaccgccg ccgcagctca tccggccacg
     4321 gctcctcggc tgcggccgcc gtactgatcc gctcgagagc tccgcggtgg attctggcgc
     4381 cgtgtcgcgc cgctgcaagc gcgacgagca gtggttcgat ggcatcgacg ctgctcggcg
     4441 cgtccggcgc cacgaccacg aacccaccat cgagctcgta gggaccctcc agcgacggcg
     4501 atcgccgcgg tcggagcctc gagctggggc gcagccagcc ttgcaggcga ctgcaggcgt
     4561 catcggccgc ccatgcgatg cggcgggcac tgcccgcaag ctccgacatc aactccatcg
     4621 cgtcggcgaa tccaaggcgc gacgcaacct ccgcctgatc gtcgagccgg atcacgtcgc
     4681 cgggccggtt gctcagacgg tgcagttcga cgcgtaccgc caacaccagg tcgtagtcct
     4741 cggccaggtc gtcggccgag gcgatgtcca agccctggtc cgcagcctcg atccagccca
     4801 gcaggtggac atcgcgcagg ccgccccgac cctccttcaa gtccggttcg agcgcgttgg
     4861 cgacactgct cgcatcagcg tgacgacgag tggccgtgtc gacgagctcc ccgaaggccc
     4921 gtttggcgcg acgcttccac tcgacgaggt gggcctcgac gagcgccgcg gtcaatgacg
     4981 cgtcgcccgc gagatggcgc gcactcaaca gcccggtagc cgtcgccagg tcggtcgctg
     5041 cgagttgcag ggtttccgcc ggggtccgca ccgcatgtcc gaccttcatc ttcgcgtccc
     5101 acagcggata ccacagcgac tgagcggtgg agccgtcgag cgaaccatcg tgtagcaaca
     5161 gcaggtcgag gtcgctgaac ggcgacagct ccccgcgccc ttgcccgccg gtcgcgacga
     5221 gcgctacccc atcgtcgaga ccgaccaaat cttcgtagag cgagcacagc catcgttcga
     5281 cgacggcggt gtagtcggtc gtaaaggccc ggcccaacag gtcgctgcgt tgaagcaggg
     5341 cggcgagttc ggtttccacc ttcatgagcg ggtgcctccg tcggatcctc gggtgggtcg
     5401 aacctaccgc cgacgcatca ccgccctgcc tgcggatggt gaacgcagcg tgaacaggtc
     5461 gcgcgccgaa tgggtcgggt ggacccccct acggggcttg gcgccgagcc cgcttgtgca
     5521 ggaacgcctt cgccgccccc cacgccatac cggcgtcgag cacctcctcg ccgaagtcag
     5581 cccatgagcc cggcgcaacg ccaaagaggt catcgggatt cacccgttct tcgaatggat
     5641 cgcgctgcac cggaattgct ccggcctcgc tcaacaccgc cgtcgggtag cgatgcgcgc
     5701 cccgaaggat cgcgagcggc gtcgtcgact gctcgtcgac cggaacggcg agcagctcgg
     5761 cgaggcgaac gtgcacgtcg gtagccgcct cgtcggccgc gccccgcgcc gcgtcgagaa
     5821 ccgccgccgc acgagtggcg tcgaggccgg agatccgttc ggcgaccacg tcgagtaccc
     5881 accgatgcac gccgacggtg gccacggcga tgaggcggtc ggacaactcg cgcatcgcag
     5941 caaggtcggt gtcaaacggc gccgtgttgc cgttggggtt ggcgtggtcg ggctctgccg
     6001 gcctgtcgga cgagggttgg ctcacagctc tttgctagtc gatgtcgccg cgccgtagct
     6061 cgctgagcgc tgccctcacg tgggagaacc ggatccgctc ctgttcaagc cgcagcgatg
     6121 cgccatagcg gtcctccacc agatcacggt aggtcgccga ctcctccgcg gtgaggtggg
     6181 tgagcatggc tggctttgcc ttcgcgtcgt tcaccacatg ggcaagatgc gagctgagtg
     6241 tggcgcgatc catcaacatt gactcgacgt tgccgacccg ctggcgaacg gcgtccagga
     6301 tggcgaaacc cgcgaggtcg aggtcccccc agtagagcac ccgtcgttcg gccagccagg
     6361 cgatatcgcc gagttgggtt gccccaaagc ctccgccgaa gatgacgatc gaccgtggga
     6421 ccgggggaaa cgcgaggtac gtcgcctggt tctcgacgat gaagacctgc tccaccgaca
     6481 gcggcatcgc cgcgagttcg tcggttcgca aggtgagctc gctgattcca tccggcagtt
     6541 cctcgacctc gtcgagcaac cgaaacctca cgtagcgagg tggccgacga aatccgtacc
     6601 gcgcaacgaa ctcggattcg atggggttga cccgttcggg gggcaacacc acatcgagca
     6661 acgacccgat gatcgcaccg tgggcggtga tgaacttggt gtcgatcccg tattgcgctc
     6721 ccgctccccc gacatcgaca tggcgcaggt cgagccccga gacatcggtc gccgcgatcc
     6781 actgagtgac cgcgatgatt cccggccacc acggttgcga tccgagtgcc gggtgtgggt
     6841 gttgagcgac ccaccgcgcc gcttcgtcgc cgagcgccgc tccggtcgcc gccaccaact
     6901 gctcggcctg ttgcagttcg cgacgcacgc cgagcgcggc aatgagttgg tcgatcgaat
     6961 cgaaccacac ggcgaccgga atctcgttct ccccgatcga tcgggtcttc atcgttcgag
     7021 tctcgacccg gccgtgaggc gcggccgccg aatgcaccgc atcggcccaa tcgatgacct
     7081 cactgaaatg ctgagacagg tcggctgcct tcggaccgcc gagcgaaacc ctcaccggct
     7141 caaagggttc gcccagcagc gcttcacgga ggtaccggcc acgctgccac cgcgcgttca
     7201 gggttgcgac cacatcccgg tacgtcttcc agcgcggcct cggcccggat ccaccgctcg
     7261 cggatgccgg gttcacctcg cgcggcctca cgcctgcccg gcggattcac ggcggcgatg
     7321 gtgttcgctc atcgtcatct gcaccagttg ggagttgttc tcctcgggat tgacgacgta
     7381 gccgaccgtc gacacgaacg gttcgatcac gcggatcttc tgcaacggcg tcacgatgag
     7441 caactgcagg ccgagtgtgc gaaagagccg gaggccgaac cgggttgact catcggagcc
     7501 gcggccgaac gcctcgtcga tcgccacgaa gcgaaacgat ccggcacccg cggtcgaacc
     7561 gtgactgaac tggtacgcga gcgaggccgc cagcacggtg taggcgagct tttccttctg
     7621 gccgccggac ttaccggagg agtccgcgta gttctcgtat tcggtgtcgt cgcttcgcca
     7681 tcgctccgat gccgaaaaca cgaaccagtt gcgaacgtcg gtcacccgct tcgtccaagc
     7741 ccggtcctgg tcggtcgatc cctcccggcc gacgaaacga tcgatcaacg ccttcacctg
     7801 taagaagcgg tgctcggagt agtcgtcgtc gccagggttc gtggtacggc tcgtcacggc
     7861 ccgcagctcg tcgcgaaagc cgcggatttc ggtgttcggt gtcgcttcgg gcagtagccg
     7921 gatgaaacgc ccggggttgt aatcgatctc gtggagcgac ccgttgatcg tgtcgatgtg
     7981 gcggcgaatg tcgcgctgac ggccttcgag gtcggccgaa agctgcgcga tctccttgat
     8041 cgtgttctcg cgcaacgagg tccggaattc cgcctcgaaa cgaggtaggt cgtcggtgcg
     8101 cacgcgttcg tacatctcgc ggtattccgg ccccgcggcc accgacgcgt cgagttccgc
     8161 agtctcacgt tggaagcgtg ctcggaaacc ggccatggcc cgctcggcct gacgctctgc
     8221 accatgtgca cgctcggtcg cgcggtgctg ggcacttcga aggccctccg ttgcaccgct
     8281 gcgggcgctg tcgattccgt cgagttgcag cgcgcctcga cgatgctcga tgctgaggtg
     8341 ttcctcgagt tcatcgaagt acccgcgggc cacctcggct tcgacaagac ccaagagctg
     8401 ctgcagatcg ttgcgctggg cctcggtccg ctcggcctct ccggtcaccc gtccgatctc
     8461 ggttttcacc gagtcgcgtt cggttgattc ctcgacgatc ctggccacga cggcgtcgag
     8521 ttgggcggtc agcgcctgca gctcgaccga cgctg
//
LOCUS       DAOOMA010000032         8435 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_9870_length_8435_cov_4.260143, whole genome shotgun sequence.
ACCESSION   DAOOMA010000032 DAOOMA010000000
VERSION     DAOOMA010000032.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 8435)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8435)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8435
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9870_length_8435_cov_4.260143"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1906)
                     /locus_tag="PLP95_01765"
     CDS             complement(<1..1906)
                     /locus_tag="PLP95_01765"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HPB44557.1"
                     /translation="MITVSGIAKAHGNRTLFSDVTFRLLPGRRIALVGGNGVGKTTLL
                     EIVVGLQDADGGEVHRTKGLRVGYLPQEVLAENAGSVLDEVLSGADHIRDLEAELGGL
                     LDTIAAAEGSERDRALDSYGELQSRFEQLGGYGLESEARRILSGLGFADTDADRPFRE
                     LSGGWRMRAALARLMLSRPAVLVLDEPTNHLDTDSIAWLEAQLAGYEGAILFVSHDRD
                     FIDAVAERVIEVIGGGATEYVGGFAEFVVQRSERVAQLRAAQAQQQRQFDQANRFIER
                     FRYKATKARAVQSRIKALEKIEKIELPDHRQIVAKFSFPEPRRSSRVVAELAGVSVGY
                     GGEEVLTGVDLVVERGEKIALVGPNGAGKSTLLKLLMGELEASAGTLQMGNNVDIAYF
                     AQHQVESLDLTRTVAMEFTAQVGNQQPKNRNLRTVLGAFGFPGESADRLVGDLSGGER
                     TRLALAICMTNPVNLLILDEPTNHLDLPSCDVLEDALSAYPGTVLLVSHDRYLIRSVA
                     QDLLEVRNGSVRRHHGVDETVLTPSFAGGTAAASPTKSDPAPSKPTAVAKPAAERTPD
                     KQRRSSGSNESNRDHQRRKELRKTVARLERELHSAEAKVAELNRELSDPAVYGDHERA
                     TELAEVFGAAK"
     gene            1980..3245
                     /locus_tag="PLP95_01770"
     CDS             1980..3245
                     /locus_tag="PLP95_01770"
                     /inference="COORDINATES: protein motif:HMM:NF017760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HPB44558.1"
                     /translation="MDEHDVEHDTGRRAGWRQFRTYWFGHVASFTGEWFTNLALPYAA
                     LSINDSPFFIGVIESAELISTLVVGLYLGHLADRMSPRRVLITSDLVRSAALLTVALS
                     FAFGAPPALLLVAVAFVLGAMRDFHDSGEGVMMVSVVDERDLVRANGWFQISDGFGHM
                     TGSLLAGVAASIGLVVAFAVDATSYALAAVAVVAMGRVQVNRSESGVADGPTEGPPEA
                     ATSWRVVATALRRDNRYWRVLTTMALTNVASACFMGQFVNFADLELGISQWQVGLTWS
                     VMGAGSFLAGLWLDRARNITPRSILAAPAVMGGGLIAVGQSHSWVVTLIVFGAVGFVL
                     AWGAALIAAMRHASFPAEIQGRVAMVTRLAFAMGIIPAVLGAGALASTIGSSRTFIAF
                     GTLAAVGLLLGLFLRVTDFESLTRPQTTQ"
     gene            complement(3474..4442)
                     /locus_tag="PLP95_01775"
     CDS             complement(3474..4442)
                     /locus_tag="PLP95_01775"
                     /inference="COORDINATES: protein motif:HMM:NF015509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HPB44559.1"
                     /translation="MIAAFDMAAFGHNAVRLLVLVVPTAAGLLLARRRVVPPEDRIIF
                     GLNAFVLYVCFPALIALGVLRVDLHAISGWGFWTVIGVTTLLAVAVGWLSTPLSRARI
                     AGTTTLVLLFGNTAFIGIPFVIGVFGDDLRGPATVIVAVQVTIAVLIGPILLHRWSGT
                     DRSSRVWRRALAQPLMWSPLFGIAMRVLPPAVSTEAVNALDPLAVAAAPTSMFVLGVH
                     LARSRGDGEATNVSGVVFSCVTRLVVLPAVTLALCAPMVSSGSLEPGLATVFVVLAAC
                     PAAVSTFSIAENEGVGAATVASTILWSGALCLVSLPLWAMIADAMW"
     gene            4488..4862
                     /locus_tag="PLP95_01780"
     CDS             4488..4862
                     /locus_tag="PLP95_01780"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HPB44560.1"
                     /translation="MSNEKPSVTIPDTAAPSELVIEDLIVGDGTEAVAGAVVSVHYVG
                     VAWSTGQQFDASWDRGDTFEFRLAGGQVIAGWDQGVQGMRIGGRRRLTIPAHLGYGEH
                     GAGGVIKGGETLVFVVDLLNVG"
     gene            complement(4906..6132)
                     /locus_tag="PLP95_01785"
     CDS             complement(4906..6132)
                     /locus_tag="PLP95_01785"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HPB44561.1"
                     /translation="MTTIAYLTGEYPRATDTFIQREVRAVEAAGVKVERFAVREPGSE
                     HLVGTMQREERGATTYLLGHGSAALVRSALSALWRAPHRFVRAASLAARTRRAGLDGV
                     MRQLAYFAEAAVLAEEMRRRKVEHLHNHFGDSSCTVAMLASELSGIGYSFTLHGPAIF
                     FEPHEWHLGTKIERARFVATISNFARSQAALFCSPDAWGHLYIVHCGVSEPEAIDVDG
                     SGWPRSEGTTDLLFVGRLDPVKGVMVLFETIAQLVEAGENVRLVLVGDGPQRVELEEL
                     TADLGLSDRVRFTGYQNQQQVQAHLERCDVFVLPSFAEGVPVSLMEAMARRRPVIATN
                     VGGVTELVEDGISGRVVPPGDDVALRSAIEDLARDPQLREKMGAAGAAKVHAEFNSST
                     EAQRLVSLFDRAILSG"
     gene            complement(6129..7139)
                     /locus_tag="PLP95_01790"
     CDS             complement(6129..7139)
                     /locus_tag="PLP95_01790"
                     /inference="COORDINATES: protein motif:HMM:NF013530.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HPB44562.1"
                     /translation="MKVFITGSTGFVGSHVVAAALGAGHDVVALVRPASSDPFPTLSS
                     HPRLTLHRGDLRDPRTIAGAFDGVDVVVHLAAAKAGDFATQFAGTVIATENLIDELAR
                     SRVRRLVAISTFSVYDFSAIEAGSLIDETSPIDGSPTDRDEYAQTKLIQERLYREEMA
                     HREVVILRPGMIYGRDNLWHPLLGSELGPVFLRIGSRATMPMTYVENTAAAIVAAIDA
                     DGVGGETINIVDDDLPTQKDYARDVEAYVTPPSSVPVPWPVMSGIAHLLDRMNRSMLG
                     GRAKFPGVLVPAKLDGRFKPFRYSNVKAKRLLAWTPTISQRDAFVRSLRDDVLTEVAG
                     RT"
     gene            complement(7136..8266)
                     /locus_tag="PLP95_01795"
     CDS             complement(7136..8266)
                     /locus_tag="PLP95_01795"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HPB44563.1"
                     /translation="MTLPPSAQRRLTAAVLGTGGISKEHLGFLAASRDIDLVGVCDRS
                     PAALRHAAETYSTTGFATFGEMLDQAQPDVVHVLTPPATHSALSAEALEAGSHVICEK
                     PITATAAELQDLLAVARRCDRVLTENHNYRFNPEVEALVALTGDGPLGDVVEVEVRIH
                     LPVTDPAGRFGDPNLPSPIHDLPAGVVHDFITHFTYLVNYLSGGARWERVASEWSAHC
                     GAAHIPVDDLDAILVGSAPWGPVHGRIRFSALASPDEFEVTVRGANGYAQAELFSGRW
                     VENRPRRVGDQLNSIANLMIGGVNQFLGGPRSFARKIMQAGPYAGLNRFLEETYRSLA
                     NETPVPVGPEEMLAASELIDRLVAGRRESASDLTETTREGQR"
BASE COUNT         1450 a         2854 c         2684 g         1447 t
ORIGIN      
        1 ccttcgccgc tccgaacacc tcggcgagtt cggtggcgcg ttcgtggtcg ccgtacacgg
       61 ccggatcgct cagttcccgg ttcaactccg cgaccttggc ctcggccgag tggagttcgc
      121 gttcgagcct cgccacggtc ttgcgtagct ctttgcgccg ctgatggtcc cgattggatt
      181 cgttcgaacc cgatgaccgc cgctgtttgt ccggcgtccg ctcagcggcc ggctttgcca
      241 cggccgttgg cttcgatggc gccgggtcgc tcttggtcgg cgacgccgcg gcggttcctc
      301 cggcaaagga cggggtcaac acggtctcgt cgacgccgtg atgccgccgc accgaaccat
      361 tgcgcacctc gagcagatcc tgcgcgaccg agcggatcag atatcggtcg tggctgacca
      421 gcagcaccgt tcccgggtag gcgctgagcg cgtcctccaa cacgtcgcag ctcggtaggt
      481 cgaggtggtt cgtcggctcg tcgaggatga gcaggttcac cgggttggtc atgcagatcg
      541 ccagggccag gcgggtgcgt tcacctccgc tcaggtcgcc gacgagccga tctgccgact
      601 cgcccggaaa tccgaaggcc cccaacaccg tgcgcaagtt gcgattcttg ggctgttggt
      661 tcccgacctg agccgtgaat tccatggcga ccgtacgcgt gagatcgagc gattcgacct
      721 ggtgttgggc gaaataggcg atgtcgacgt tgttgcccat ctgcagcgtg ccggcggacg
      781 cctcgagctc gcccatcaga agcttcagca acgtggactt ccccgccccg ttcgggccga
      841 cgagggcgat cttttcgccg cgctcgacga cgaggtcgac cccggtgagc acctcctcgc
      901 cgccatagcc caccgacacc ccagcgagct cggccacgac tcgcgagctg cgacgaggtt
      961 ccgggaagga gaacttggcg acgatctggc gatggtccgg tagctcgatc ttctcgattt
     1021 tctccagcgc cttgattcgg ctctgcaccg ctcgagcctt ggtcgccttg tagcggaacc
     1081 gttcgataaa gcgattcgcc tggtcgaact gccgttgttg ctgggcctgc gcagcacgaa
     1141 gctgggcaac ccgctcggag cgttgcacca cgaattccgc aaacccaccg acgtactcgg
     1201 tcgcgccgcc accgatcacc tcgatgacgc gttcggcaac ggcgtcgatg aagtcgcggt
     1261 cgtggctcac gaacaggatc gccccctcgt acccggcgag ctgagcctca agccatgcga
     1321 tggagtcggt gtcgaggtgg ttggtcggct catcgagcac gagcaccgca gggcgcgaga
     1381 gcatcaaccg ggcgagcgcc gcccgcatcc tccatccgcc cgagagctcg cgaaacggac
     1441 gatccgcgtc ggtgtcggcg aagcccagac ccgacaggat gcgacgagcc tccgactcga
     1501 gcccgtagcc cccgagttgt tcgaatcgag actgcagctc accgtaggag tccaacgccc
     1561 ggtcgcgttc cgatccctcc gccgcggcga tggtgtcgag cagcccgccg agttcggctt
     1621 cgaggtcacg aatgtggtcg gcgcccgaca acacctcatc aagcacggac ccggcgtttt
     1681 ccgccagcac ctcctggggg aggtagccga cccgcaaccc cttggttcgg tgaacctcgc
     1741 ccccgtcggc atcctggagg cctacgacga tctcgagcag cgtggtcttt ccaacaccgt
     1801 taccgcccac caaggcgatg cgacgccccg gcagcagccg aaacgtgaca tcggaaaaca
     1861 gggttcggtt gccatgagcc ttggcgatgc cggagacggt gatcacgaag ggaccatgct
     1921 atggcaacga caccggctcg ccgcacccgg acccgccgac cgatagcgtc tgtgcccgga
     1981 tggacgagca cgacgtggaa catgacaccg gccgccgggc agggtggcgc cagttccgga
     2041 cctactggtt cggccacgtc gcgtccttca cgggggagtg gttcaccaat ctggcgttgc
     2101 cgtacgccgc gttatcgatc aacgacagcc ccttcttcat cggggtcatc gaatccgccg
     2161 agctgatatc gacccttgtc gtcgggttgt acctcggaca cctcgccgac cgcatgtcac
     2221 cgcggcgcgt cctgatcact tcggacctcg ttcgcagtgc cgcattgttg acggtcgcgt
     2281 tgtcgttcgc tttcggggct ccaccggcgt tgctgctcgt ggcggtcgcc ttcgtcctcg
     2341 gcgccatgcg cgacttccac gacagcggcg agggggtcat gatggtctcc gtcgtcgatg
     2401 agcgcgatct cgtccgggcg aacggctggt tccagatctc ggatggtttc ggccacatga
     2461 ccggatcact tctggccggg gtggccgcat cgatcggtct ggtggtcgca ttcgccgtgg
     2521 acgcgacctc ctacgctctc gccgccgttg cggtcgtggc catgggccgc gtccaggtca
     2581 accggtccga atcaggcgtc gccgatggcc ccaccgaggg ccctcctgaa gccgcaactt
     2641 cgtggcgcgt cgtcgccacc gccttgcgtc gagacaaccg ctattggcgg gtgttgacga
     2701 cgatggcgct gacgaacgtc gcgagcgcct gtttcatggg ccagttcgtc aatttcgccg
     2761 acctcgagtt ggggatttcc cagtggcagg tcggactcac ctggagcgtg atgggcgccg
     2821 ggtcgttcct tgccggactc tggctcgacc gggcccgcaa catcacccct cgatcgatcc
     2881 tcgccgcccc agcggtcatg ggaggtgggc tcatcgcggt tgggcagagc cacagttggg
     2941 tagtcacgct catcgtgttc ggagccgtcg gcttcgttct ggcgtgggga gcggccctca
     3001 ttgcggcgat gcgccacgcc tcgtttcccg cggaaatcca gggacgggtc gccatggtca
     3061 cgcggctggc ctttgcgatg ggcatcatcc ccgcggtgtt gggcgccggg gcgctcgcga
     3121 gcacgatcgg atcgtcgcgg acgttcattg cgttcggcac cctcgccgca gtcggcctgc
     3181 tgttggggct gttccttcgt gtcaccgact tcgagtccct gacccggccc cagacgaccc
     3241 agtgagcact aggtcgaatt acccaagaac cagttgacca cctgatgttc ggcggatagg
     3301 ttcaggcccg catttggcga tgcggaaggg aggaaccacc atgcgtgcat ctcgtttcaa
     3361 gtggtaattc cactatcccg tggaattccc acgcacagaa cgggcctccc ctcagtggga
     3421 ggtctgttcg tttttgcgcc gacaatccgg gtcggaacgc agcggcggaa ctctcaccac
     3481 atggcgtcag cgatcatcgc ccacagcggc aggctcacca ggcacagcgc cccactccag
     3541 agaatcgtcg aggccaccgt cgcggcgccg acgccctcat tctcagcgat cgaaaacgtg
     3601 gagaccgccg cagggcaggc cgctagcacc acgaacaccg tggccaggcc tggctcgagg
     3661 gatccggacg acaccatcgg ggcacacaac gccaacgtca ccgccggaag tacgaccaat
     3721 cgggtgacgc acgagaacac gacgcccgac acgttcgtgg cctcgccatc gccgcggctc
     3781 cgagccaggt gcaccccgag cacgaacatc gacgtcggag cggctgcgac cgcgagcgga
     3841 tcgagcgcgt tgaccgcctc ggtcgacacc gctgggggaa gcacgcgcat ggcgatgccg
     3901 aacaggggcg accacatgag cggctgggcg agcgctcgcc tccacaccct cgacgaccga
     3961 tccgtgccgc tccagcgatg caacagaatc ggcccgatca gcacggcgat ggtgacctgg
     4021 accgcgacga tcaccgtcgc cggtccccgc aggtcgtcgc cgaagacgcc gatgacgaac
     4081 gggatcccga tgaaggccgt gttgccgaac aacagcacca atgtcgtcgt tccggcgatc
     4141 cgggcacggc tcaggggggt actcaaccat ccgaccgcga ccgctaagag cgtcgtcacc
     4201 ccgatcacgg tccagaaccc ccaaccggag atggcgtgca ggtcgacccg caacactccc
     4261 aatgcgatca gcgccgggaa acacacatag aggacgaacg cgttcagacc gaagatgatc
     4321 cggtcctccg gaggcaccac ccgccgtcgg gcgagcagta gtccggccgc tgtcggcacc
     4381 accagcacca acagccgaac cgcgttgtgc ccaaaggcgg ccatgtcgaa tgcggcgatc
     4441 atcgcgccca tccttgcgca tcacaggaac ccgatagcgt gtgcgccatg tccaacgaga
     4501 agcccagcgt caccatcccc gacaccgccg caccgtcaga gctcgtcatc gaggacctca
     4561 ttgtcggcga cggcaccgag gcggtcgccg gggcggtcgt gtccgtgcac tacgtggggg
     4621 tcgcctggtc gaccggccaa caattcgacg cgagctggga tcgcggcgac accttcgagt
     4681 ttcgcctcgc cggaggtcag gtcatcgctg gttgggacca gggagtgcag ggcatgcgta
     4741 tcggcggccg ccgccgcctg acgattccgg cacacctcgg atacggcgaa catggcgccg
     4801 gcggagtcat caagggcggc gagaccctcg tgttcgtcgt cgatctcctc aacgtcggct
     4861 gagccctgtc atcgtcggac cagccctcgt cgacactcca cgaggtcacc ctgacagaat
     4921 cgcccggtcg aacaggctca cgaggcgctg tgcttcggtc gaggagttga actcagcgtg
     4981 caccttggcg gcacccgccg cccccatctt ctcgcgcaac tgcgggtcgc gtgccaggtc
     5041 ctcgatcgcg gagcgcaacg cgacgtcgtc tccgggcggc acgacgcgcc cgctgatgcc
     5101 gtcttccacc aactcggtga cgccgccgac attcgtggcg atgaccggtc gacgacgggc
     5161 catcgcctcc atgagcgaca cgggcacgcc ctcggcgaag ctcggcagca cgaatacatc
     5221 acagcgttcc agatgcgcct gcacctgttg ttggttctga tagcccgtga agcgcactcg
     5281 gtcgctcaga ccgagatccg cggtgagctc ttcgagttcg acgcgctgcg gtccgtcgcc
     5341 caccagcacc agccgcacgt tctcgcccgc ctcgacgagc tgagcgatcg tctcgaacaa
     5401 caccatgacg cccttgaccg ggtcgagccg accgacgaac aacaggtcgg tggttccttc
     5461 cgaccggggc cagccggaac cgtccacgtc gatcgcttcg ggttcactca caccacagtg
     5521 aacgatgtat agatggcccc acgcatcggg gctgcagaac aacgccgctt gggaacgtgc
     5581 gaagttcgag atggtcgcta cgaatcgcgc tcgttcgatc ttcgttccga ggtgccactc
     5641 atgcggctca aagaagatgg ccggtccgtg aagcgtgaag ctgtatccga taccgctcag
     5701 ctcggaggcc agcatcgcaa ccgtgcagct cgagtcaccg aaatggttgt gcaggtgttc
     5761 aaccttgcgg cggcgcatct cctcggccaa caccgccgct tcggcgaagt acgccagctg
     5821 gcgcatcact ccgtcgagtc cggctcgccg cgtccgcgcc gccaacgatg cagctcgaac
     5881 gaaccgatgc ggggcacgcc acagcgccga caaggcggat ctcaccagcg cagccgaacc
     5941 atgaccgagc agataggtgg tcgccccccg ctcctcgcgt tgcatcgttc cgacgaggtg
     6001 ttcggatccc ggctcgcgca cggcgaatcg ttcgaccttg acccctgcag cctccaccgc
     6061 ccgcacctcg cgctgaatga acgtatcggt tgcccggggg tattcacccg tgagataggc
     6121 gatcgtcgtc atgtccgccc cgcgacctcg gtcaggacat cgtcacgcaa cgaacgcacg
     6181 aacgcgtcgc gttgggagat ggtcggggtc catgcgagca ggcgcttggc cttgacattc
     6241 gagtaccgga acggtttgaa acgcccgtcg agtttcgccg gaacgagcac gccggggaac
     6301 ttcgcacgtc caccgagcat ggagcggttc attcggtcga gcaggtgagc gattccgctc
     6361 atcaccggcc acggcaccgg aaccgacgag ggaggtgtca catacgcctc cacgtcgcgc
     6421 gcatagtcct tctgtgtcgg aagatcgtcg tcgacgatgt tgatcgtttc gccgccgacc
     6481 ccgtcggcgt cgatggcagc gacgatcgcc gctgcggtgt tttcgacata ggtcatcggc
     6541 atcgtcgccc gggaaccgat ccgcaggaac accggcccga gctctgatcc gaggagtgga
     6601 tgccacaggt tgtctcggcc gtagatcatt cccggccgca ggatgaccac ctcgcggtgc
     6661 gccatctcct cgcggtacaa ccgctcctgg atgagcttgg tctgcgcgta ctcatcgcgg
     6721 tctgtcggcg acccgtcgat cggcgaggtc tcatcgatca gcgatcccgc ttcgatcgcc
     6781 gaaaagtcgt agaccgaaaa ggtgctgatt gccacgagcc gcctcacccg actccgcgcc
     6841 aactcgtcga tcaggttctc ggtcgcgatc acggttcctg cgaactgcgt cgcgaagtcc
     6901 cccgccttcg ccgcggccag gtgcaccacc acgtcgacgc cgtcgaacgc gccggcgatg
     6961 gtacgggggt cgcgcagatc gccacggtgc agtgtcagcc tcggatggga ggacaacgtc
     7021 ggaaagggat cggaggaggc cggacgaacg agcgcgacca cgtcgtgacc tgcaccgagt
     7081 gcggccgcga ccacatgact tccaacgaac ccggtcgagc cggtgatgaa caccttcatc
     7141 gctgcccctc ccgggtcgtt tcggtgagat cggaggcgct ctcgcggcgg ccggcgacga
     7201 gtcggtcgat cagctcgctc gctgcgagca tctcctcggg gcccaccggg accggcgtct
     7261 cgttggccaa cgaacgatag gtctcttcca aaaaccggtt gaggcctgcg tacgggcctg
     7321 cctgcatgat cttgcgggcg aacgaacggg gaccgccgag gaactggttg accccgccga
     7381 tcatcaggtt ggcgatcgaa ttgagctggt ctcctacacg ccgtgggcgg ttctccaccc
     7441 atcggcccga gaagagttcg gcttgcgcgt acccgttggc gcctcgcacc gtcacctcga
     7501 actcgtcggg ggatgcgagc gcgctgaagc gaatccgtcc gtgcaccggg ccccatggcg
     7561 ccgacccgac gaggatcgca tcgaggtcat cgacggggat gtgcgccgct ccgcagtggg
     7621 cgctccattc gctcgcgacc cgctcccacc gggccccacc gctcagatag ttcaccagat
     7681 aggtgaagtg cgtgatgaaa tcgtggacga ctcctgccgg caaatcgtgg atcggactgg
     7741 gtagattcgg gtcgccgaaa cggccggcgg gatcggtcac tgggagatga attcgcacct
     7801 ccacctccac gacgtcgccg agcggcccgt cgcccgtgag cgcaacaagc gcctcgacct
     7861 cggggttgaa ccggtagttg tggttctcgg tcagcacccg gtcacatctc cgggcaaccg
     7921 ccaacaggtc ctgcaactcc gctgcggtcg cagtgatcgg cttttcgcag atcacatggc
     7981 tgcccgcctc cagtgcctcc gcggacaacg ccgagtgagt ggccggcggg gtgagcacgt
     8041 ggaccacatc tggctgggct tgatcgagca tctcaccgaa ggtggcgaag ccggtggtcg
     8101 aataggtctc ggcggcgtgg cgtagcgccg ccggcgatcg gtcgcagacg ccgacgaggt
     8161 cgatatcgcg tgacgcggcg aggaatccaa gatgctcctt ggaaattcca ccggtgccga
     8221 gtacggccgc ggtcagtcgt cgttgggcag aagggggaag ggtcaagggg gctccttgcc
     8281 gtggtcgacg tcggggtgcc gacgacagga catgttacgg aggtctcggc gattcgccac
     8341 cctcgacccg caacaagttg cgcgagcgtc agcccctgct gtttcgagcg gcggccgacc
     8401 gatcgctcgg atggagatgt gggcgcgaat cggga
//
LOCUS       DAOOMA010000033         8376 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_9966_length_8376_cov_4.028963, whole genome shotgun sequence.
ACCESSION   DAOOMA010000033 DAOOMA010000000
VERSION     DAOOMA010000033.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 8376)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8376)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8376
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9966_length_8376_cov_4.028963"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(110..1621)
                     /locus_tag="PLP95_01800"
     CDS             complement(110..1621)
                     /locus_tag="PLP95_01800"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HPB44564.1"
                     /translation="MLKPAAARPSEHDASEFEASESSKRRSLRLVGRDGGFASDSHGD
                     AAPFTQQQRRGIGAWALLLGVALDAVIVLGVAYLTRRGSGSDRDLGNIILAYTAISTV
                     LLLPTHRRGRVVPRPSETALSVVVRIGLAPLIASVLAGWVSAVDITVWAQLVAITSAL
                     VLLGRLVSFKLVQGLRSRGYDLEDVILVGAGAVGRDLAEAIDQNPDCGLLPIGFLDRF
                     DERGRLPVIDRPENLAEVCAERNIRHVILGYGAATEHEIVGYVRQCAAMPIQFYTVPR
                     FFELGVASGATGFEVDGFAITTLGRAGRHHLMWPLKRAFDLVVTSVMFILTLPVFIVV
                     AAAVKLTSPGPILFRQERVSVDHVPFEILKFRTMAHVADPKKQAELDQRAQAVNLDDD
                     RITPIGRFLRKSHLDELPQLINVLKGEMSIVGPRPERPYFVDQHSEEYPAYAHRHRVP
                     AGITGWAQVNGFWGDSSLETRVRLDNRYIDDWSPVRDLVIGLRTIPTLLGKRR"
     gene            complement(1772..3124)
                     /locus_tag="PLP95_01805"
     CDS             complement(1772..3124)
                     /locus_tag="PLP95_01805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB44565.1"
                     /translation="MSELGTTAVGTLATLPDPTLVEEHNPISFRFPRRTWLLIGLATL
                     VAGVLASLATLALPEQYAVTRTLFVMNSSNPTDIDTLTRLLEELIADKGFAMELKQMA
                     DVDLPVEKVKSMVSTNRPPLSASLDVVVRAADRETAATLSAAIVPTLEKVFETPFKNV
                     PISSRIATPVVLDMWEGGVAEQEYVPWWMGFVAGAAVAFMFGFVIAAYRQYRRPVIAS
                     ARDVGEALDLPVLARVATIGDGRGTNPSDAVLGMLSAIERLGTAGPIHRLVVVGPEAD
                     LERSKLVLALGCAIARNFDQPVAIVDADLQNASLSKLVGAIDEPGLAECLSGDLRVDQ
                     TLLRIENGHMPAVLDSMAPPAGMIRLMPAGIDRSGNLLRMRSNLHQVLGTMSGKYVVI
                     IDGPQVPGSVPTHQLLTLADATVVVVTEGGTSIRDARFTGNAVRSNTTNPVGAVVLRR
                     "
     gene            3334..3435
                     /locus_tag="PLP95_01810"
     CDS             3334..3435
                     /locus_tag="PLP95_01810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003402602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AURKAIP1/COX24 domain-containing protein"
                     /protein_id="HPB44566.1"
                     /translation="MGSLIKKRRKRMRKKKHRKLLRRTRHQRRARGK"
     gene            complement(3472..4191)
                     /locus_tag="PLP95_01815"
     CDS             complement(3472..4191)
                     /locus_tag="PLP95_01815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020378437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HPB44567.1"
                     /translation="MIELLAPAKLTVSLRLTGVRPDGYHLIDAEMVSLDLCDRLWMSP
                     GRGVEMRGRYAGELAGPTDGTDLVSKALRLIGHEASVVVEKNIPAGAGLGGGSSDAAA
                     ILRWANFDDLAAAAELGADVAYCLVGGRARVRGIGEIVEPLDERRQTFTLVTPPVHCS
                     TPVVYRVWDELGGPTAPMDAVEPNDLTEAALMAFPEIARWRDELAEASGLTPRLAGSG
                     STWFVEGSFPGAGRQVVTTLS"
     gene            complement(4203..5063)
                     /gene="rsmA"
                     /locus_tag="PLP95_01820"
     CDS             complement(4203..5063)
                     /gene="rsmA"
                     /locus_tag="PLP95_01820"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: protein motif:HMM:TIGR00755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HPB44568.1"
                     /translation="MTEPRPGSTLPLLTRTRLLEVMAEHDLAPHRTLGQNFVIDHNTI
                     RKIVRLAGVQPDDVVVEVGPGLGSLTLGLLEAGASVRAVEIDARLVPAMQAITAGAAE
                     VTVADALEVDWSKVTRGEPCTLVANLPYNVATPVVLRILDEAPEIGRLVVMVQKEVAE
                     RLAATPGSKAYGIPSVKVAYWATARMVGTIGSEVFHPRPKVTSALVEIIRRPQPVDGS
                     DPTALFALVKAGFGQRRKTLRRSLSSLVDDEAFVAAGVSPSARAEELDVVAWCRLAAA
                     VRNKAESALS"
     gene            complement(5060..5674)
                     /locus_tag="PLP95_01825"
     CDS             complement(5060..5674)
                     /locus_tag="PLP95_01825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB44569.1"
                     /translation="MTPMRGAQNSPGESVRRVFIIVLSVLTVVAVPWVIIGKIRDGRS
                     ESTAATSTTAQVGSTSVETTLIESPTTVAEGGDVVQVALNPRWVQRGTSRYSETDAQR
                     RERIRRQQLGAVAPSESAGRGDSTVDASEPTPQSGSVTTTVAPPKKSTTTAAAGTAPA
                     ATATPTTAPIPTTAVTVPEVDPVEPPVSIEPPPAVAPALEVLSP"
     gene            complement(5671..6477)
                     /locus_tag="PLP95_01830"
     CDS             complement(5671..6477)
                     /locus_tag="PLP95_01830"
                     /inference="COORDINATES: protein motif:HMM:TIGR00010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HPB44570.1"
                     /translation="MNEAVAMWIDNHCHLPADQPELVRQLIDEAAAGGVEAFVNVGCD
                     LAGSAAAIRVARDHPSVYATVGVHPHDAKDGTDGIRDLAGGDQVVAIGECGLDYFYNH
                     SEREVQRAVFAEQIGLAHELGLPLVIHSREAWKETFEILDVEGVPRNTVFHCFTGGPG
                     DAEECLERGAILSFSGIVTFPSAEDLRQAAMMCPLDKMMVETDSPYLAPVPHRGKPNR
                     PAHVAVVGEYLAQLRGVDGEHFAQLTSATTRSFYGLGTDGGVALSRGAGA"
     gene            complement(6535..7401)
                     /locus_tag="PLP95_01835"
     CDS             complement(6535..7401)
                     /locus_tag="PLP95_01835"
                     /inference="COORDINATES: protein motif:HMM:NF012398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HPB44571.1"
                     /translation="MSSVDPQKASPMSTTSRRRYRHLIGGAMAESMSGRWAEVTLADG
                     TTAGICEADTAEVSAAVAAAIDARSEWAALAPQRRGQVLFEVAEALDGHRSHLVEELC
                     LGGTDREAAEAEVAETIDCWTHWAGWADKLAAVFGGASAVAGSFLASTTPRPIGVVGV
                     VACARRPLFASAALVAPVVVSGNAAVVLVDGFAPCGPLAVGEILAANDLAAGVINLLH
                     GPAPTLSLALADRVDAIALPEVAIGLDLVGELAERASRRFVRVVRLSDARDPRGAAEF
                     VEFSTVWHPARV"
     gene            complement(7398..>8376)
                     /locus_tag="PLP95_01840"
     CDS             complement(7398..>8376)
                     /locus_tag="PLP95_01840"
                     /EC_number="1.2.1.3"
                     /inference="COORDINATES: protein motif:HMM:NF012398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004030 - aldehyde dehydrogenase [NAD(P)+]
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HPB44572.1"
                     /translation="VVVKPSQRASLSALMFAHIRREARLPPGVVNVVTGGDDTGAALA
                     AHPALGALEFTGSAEAGKAVAQSTAKAGTRLLLELEGNSAQLIFDDAPLDQAIDAVVR
                     GIWQHRSEPWDVASRVLVEESIAEEVVDRLARRLQQVRVGDPLDNNTDVGPLDSAEQV
                     GRTEALVAAARQDGAEVVRWAKRPPERGWFVKPTLLHRVELPHRSLAEVTRGPVLSVL
                     TFRTHDEAVAIANQVASGRSAGVWTTDAARSQWVADQLHAGFVWVNSYDLVDPASPTW
                     GLARSLGGDRGGRFGVGAYLDLSADRGRCEGASAIDGSRPRHGDGGETP"
BASE COUNT         1542 a         2896 c         2616 g         1322 t
ORIGIN      
        1 cgcagggatc acgacgctca cgagcggctg gccaccggag cgcggagttg tcgaagtcat
       61 tcgagcagtc ttaccgagac ctcgtcgtgg caggaaccac agcgcgcgct cagcggcgct
      121 tgccgagcag cgtcgggatc gtgcgcagcc cgatcaccag gtcgcgcacc ggcgaccagt
      181 cgtcgatgta gcggttgtcg agacgcacgc gagtctccag cgacgaatcg ccccagaacc
      241 cgttcacctg cgcccagccg gtaatcccgg ccggaacgcg atgccggtgg gcataggccg
      301 ggtactcctc ggagtgctgg tcgacgaagt acggacgctc cggcctcgga ccgacgatcg
      361 acatctcgcc cttcaacacg ttgatcaact ggggaagctc atcgaggtgc gacttgcgaa
      421 ggaaccgacc gatcggagtg attcggtcgt cgtcgaggtt gaccgcctgt gcacgctgat
      481 cgagttccgc ctgcttcttc ggatcggcga catgggccat cgtgcggaat ttcagaatct
      541 cgaatgggac gtggtcgaca ctcacccgct cctggcgaaa caggatgggg ccgggactgg
      601 tgagtttgac cgccgcggcg acgacgatga acaccggcag cgtgaggatg aacatcacgc
      661 tggtcacgac gaggtcaaat gcccgcttga gtggccacat gaggtggtga cgcccggcgc
      721 ggccgagcgt ggtgatggcg aacccgtcga cctcgaagcc ggtggcaccg gaggccaccc
      781 cgagttcgaa gaatcgtgga accgtgtaga actggatcgg catggcggcg cactggcgga
      841 catacccgac gatctcgtgc tcggtggcgg caccgtaccc caagatgacg tggcggatgt
      901 tgcgctccgc gcagacctcg gcgaggttct cggggcgatc gatcaccgga agacggccgc
      961 gctcgtcgaa gcggtcgaga aacccgatcg gcaacagccc acagtccggg ttctggtcga
     1021 tggcctcggc gaggtcgcgg ccgaccgccc cggcgccgac gagaatcaca tcctccaggt
     1081 cgtacccacg agaacgcagg ccctggacga gcttgaacga cacgagcctg cccagcagca
     1141 cgagtgccga ggtgatggcg accaactggg cccagaccgt gatgtcgacg gcggagaccc
     1201 acccggcgag caccgacgcg atcagcggtg ccaatccgat gcgcacgaca accgaaaggg
     1261 cggtttcgct cgggcgtgga accacccgac cgcgacggtg cgtgggcagg aggagcaccg
     1321 tggaaatcgc tgtataggcg agaatgatgt tgccaaggtc gcgatcgctc ccggaacccc
     1381 gcctcgtgag gtaggcgacc cccaagacga tgacggcgtc gagagcaacg ccgagcaaca
     1441 acgcccatgc gccgataccc cgacgttgtt gctgggtgaa cggcgccgcg tcgccgtgcg
     1501 aatcgctggc gaatcccccg tctcgaccga cgagccgcag cgatcgacgc ttcgagctct
     1561 cggacgcctc gaattccgat gcgtcgtgtt cggatggcct cgcagcggca ggcttcagca
     1621 tcgatcaact cccgttggtt tcccaaacag tgccgagccc cgacgccgcg tcgtcatgac
     1681 tctcgactcc ccaagtacct caagagctaa gcacgccgcc cttcaacact caagggggtt
     1741 tcctcagttt cttcacaaat acgggttgcg ttcaccggcg caggaccacc gcgccaaccg
     1801 gattcgtcgt gttcgaccga accgcgttgc cggtgaacct ggcatcgcga atcgaagtgc
     1861 ccccctctgt caccacaacg accgtcgcat cggcaagcgt caacaactgg tgggtcggca
     1921 ccgatcccgg cacctgcggg ccgtcgatga tcacgacgta cttgcccgac atcgtgccga
     1981 gcacctggtg aaggttcgac cgcatgcgca acaggttgcc gctccgatcg atgcctgcgg
     2041 gcatcaatcg gatcatgccc gcaggaggag ccatcgagtc gaggaccgcg ggcatatgtc
     2101 cgttctcgat gcgaagcagg gtctggtcga cccggagatc gccgctgagg cattccgcga
     2161 gacccggttc atcgatcgct ccgaccaact tggacagcga cgcgttctgc aggtcagcgt
     2221 cgacgatcgc caccggctga tcgaaatttc gggcgatggc acacccgagc gccaacacga
     2281 gcttcgaacg ctcgagatcc gcctcggggc caacgaccac caggcgatga atcgggcccg
     2341 cggttcccaa ccgttcgatc gcgctgagca tgccgagaac ggcgtcggac gggttggttc
     2401 ctcggccgtc gccgatcgtc gcgacgcgag cgagcaccgg gagatcgagc gcctctccca
     2461 cgtctcgcgc cgaggcgatc accggacgcc gatactggcg atacgcagcg atcacgaacc
     2521 cgaacatgaa cgcaacggca gcgccggcca cgaagcccat ccaccacgga acgtattcct
     2581 gctcggcgac gccgccttcc cacatgtcca acaccaccgg cgtggcgata cgcgagctga
     2641 tggggacgtt cttgaagggg gtttcgaaga ccttttccag cgtgggaacg atcgcggccg
     2701 aaagggtcgc cgccgtttcc ctgtcggcgg cgcgaacaac gacgtcgagc gacgccgaca
     2761 acggtggccg gttggtgctc accatcgatt tgaccttctc gacggggaga tcgacatcgg
     2821 ccatctgctt gagctccatc gcaaagccct tgtcggcgat gagctcctcg agcagtcggg
     2881 tgagggtatc gatatcggtc ggattggaac tgttcatcac gaacaacgtc cgggtcaccg
     2941 cgtactgctc gggcaacgcc agtgtcgcca gcgacgccag aactcctgcc accagcgtcg
     3001 ccagcccgat cagcaaccag gtacgacggg gaaagcggaa actgatcggg ttgtgttcct
     3061 cgacaagggt cggatcaggc agtgtcgcca gtgtgcccac cgccgtcgtc ccgagctcag
     3121 acatgaaacc tcaacttccc tacgccaccg gagtgtccct cctgtggtcc ttcgccgtcc
     3181 ggccgtacgc gaacgggccg tgtccacgct accggtgcac gcacccgaac ggaagtaggc
     3241 gccgcgtgca agaactgtga agggtggtgg tgttgtgtcg aacaccagcg ggttcgcacg
     3301 cgtacggggg tgctcgctag agttccaccc gccatgggtt cattgattaa gaaacgccgt
     3361 aagcggatgc ggaagaagaa gcaccgcaag ctccttcgcc gcactcgtca ccaacgtcga
     3421 gcacgcggca agtaacccca gggccggaga tttttccggc cgccgaacgc gtcatgacaa
     3481 cgtcgtcacg acctgtctac cggcgccggg gaagctgccc tctacaaacc aggtcgaccc
     3541 actccccgct agccgggggg tgagaccgct cgcctcggcg agttcgtctc tccaccgagc
     3601 gatctccgga aatgccatca gcgcagcttc ggtcaggtcg ttcggttcga ccgcgtccat
     3661 cggcgcggtc gggcccccaa gctcgtccca gacccggtac acgaccgggg tggagcaatg
     3721 cacgggaggc gtcaccaagg tgaacgtctg gcggcgctcg tccagcggct cgacgatttc
     3781 cccgatgccg cggacccggg cccgcccacc gacgaggcag taggcgacat cggcgccgag
     3841 ctccgccgcc gccgcgaggt cgtcgaagtt cgcccatcga agaatggcgg ccgcgtcgct
     3901 actacctccc ccgagtccgg cgccggcggg aatgttcttt tcgacgacca cggatgcctc
     3961 gtgaccgatc aggcgtagcg ccttcgacac caggtcggta ccgtcggtcg gcccggccag
     4021 ttccccggcg tagcgacccc gcatctcaac accgcgtccc ggcgacatcc acagccgatc
     4081 gcacagatcg agcgacacca tctcggcatc gatcaggtga tagccgtcgg gccgcacgcc
     4141 ggtcaggcgc aacgagacgg tcagcttcgc cggtgcaagg agttcaatca tcgtccacca
     4201 acctatgaca gcgctgactc cgccttgtta cgaaccgccg cagcgagacg acaccaggcc
     4261 accacgtcga gttcttcggc cctcgccgaa ggggaaaccc cggcggcgac gaacgcctcg
     4321 tcgtcgacca gcgatgacaa cgaacgccga agcgtctttc gccgctgccc gaaccccgcc
     4381 ttcaccagag cgaacaacgc ggtcggatcg gagccatcga cgggctgggg tctccggatg
     4441 atttccacga gcgcagaggt caccttcgga cgcggatgga acacctccga accgatggtg
     4501 cccaccattc gcgcggtggc ccagtaggcc actttgaccg acgggatacc gtacgccttc
     4561 gaaccaggcg tggcagccag ccgttcggcg acctcctttt gcaccatgac gaccagccga
     4621 ccgatctcgg gcgcctcatc gaggatgcgc aacacgaccg gggtcgcgac gttgtagggc
     4681 agattcgcaa caagggtgca gggttcgcct ctcgtcacct tcgaccagtc gacctcgagc
     4741 gcatcggcga cggtgacctc agcggcgccc gcggtgatcg cttgcatcgc tggcacgaga
     4801 cgggcgtcga tctccacggc gcgcaccgac gcccccgcct cgagcaggcc gagcgtcaac
     4861 gagcccagac cagggccgac ttcaaccacc acgtcatctg gctgcacacc ggcgagccgc
     4921 acgatctttc ggatggtgtt gtggtcgatc acgaagttct gaccgagggt gcggtgtggc
     4981 gcgaggtcgt gttcagccat cacctcaagc aaccgcgtac gggtcaacag cggtaacgtc
     5041 gaaccgggtc gcggctcggt catggactga ggacctccaa ggccggagcc accgccggcg
     5101 gtggttcgat cgacaccggc ggctcgaccg gatccacttc gggcaccgtg acggcggtcg
     5161 tcgggatcgg ggctgtcgtc ggggtggcgg tcgcggctgg cgccgtcccc gcggcagcgg
     5221 tcgtcgtcga cttcttcggg ggcgcaaccg tcgttgtcac cgaacctgat tgcggcgtcg
     5281 gttccgaggc gtcaaccgtc gagtcgcccc gccccgccga ctccgacgga gcgacggctc
     5341 cgagctgctg gcgtcgaatc cgttctcgcc gctgcgcatc ggtttcggag tagcgactcg
     5401 taccccgctg aacccatcgc ggattcaacg cgacctgcac cacgtcgccg ccttcggcga
     5461 ccgtcgtggg cgactcgatc aacgtcgtct ccaccgaggt ggaaccgacc tgcgccgtcg
     5521 tcgacgtcgc ggccgtggat tcggatctac catctcgaat cttgccgatg atcacccagg
     5581 gaaccgccac gacggtcaga acgctcaaca cgatgatgaa aacgcgtcga accgattcgc
     5641 ctggggaatt ctgcgccccg cgcatcggcg tcatgccccc gctccgcgac tcagcgcaac
     5701 tccaccgtcg gtccccaacc cgtagaacga ccgagtcgtc gccgaggtca gctgagcgaa
     5761 gtgttcacca tcgacccctc ggagttgcgc gaggtactcc ccgacgactg cgacgtgcgc
     5821 gggacggttc ggcttgcccc ggtgcgggac cggggcgagg tacggcgagt cggtctcgac
     5881 catcatcttg tcgagcgggc acatcattgc cgcttgcctg aggtcctcag cggatgggaa
     5941 ggtcacgatt ccgctgaagc tcaaaatggc gccgcgttcg agacactcct cggcgtctcc
     6001 cggacctccg gtgaagcagt gaaacaccgt attgcgaggc accccttcga catcgaggat
     6061 ctcgaaggtc tccttccacg cctcgcgcga atggatgacg agcggcaagc caagttcgtg
     6121 ggcgaggccg atctgctcgg cgaacaccgc tcgttgaacc tcgcgttcag aatggttgta
     6181 gaagtaatcg agtccacatt ccccgatcgc cacgacctgg tcgccgcccg caagatcgcg
     6241 aatcccgtcc gtgccgtcct tcgcgtcgtg ggggtggacg ccgaccgtcg cgtagaccga
     6301 agggtgatct cgggcgactc ggattgccgc cgctgacccg gcgaggtcgc atccgacatt
     6361 gacgaaggcc tccacgcccc cggcagccgc ctcgtcgatc agctgacgca cgagttccgg
     6421 ctgatcggcc ggaagatggc agtggttatc gatccacatc gctacagctt cgttcacagc
     6481 cacccggtgg gtaaaacccc accaagacca cgctcagctt gatcggcgac cacatcacac
     6541 gcgggcgggg tgccacaccg tcgagaactc gacgaactcg gctgcaccac gcggatcgcg
     6601 ggcatcgctc aggcggacga ccctcacgaa ccgcctgctg gcccgctccg ccagttcccc
     6661 caccaggtcg aggccgatgg ccacctcggg cagcgcgatc gcatcgaccc ggtcagcgag
     6721 ggccaatgac agcgtcggcg ccgggccgtg taacaggttg atcactccag cggcaagatc
     6781 gttcgctgcg aggatttcgc cgacggcaag cggaccgcat ggggcaaatc cgtctaccag
     6841 gaccaccgcc gcgttgcctg aaacgacgac cggagccacc aacgctgccg acgcaaacag
     6901 agggcgtcgg gcgcaggcga caacgccgac aacaccgatc gggcgcgggg tggtcgaagc
     6961 gaggaacgac ccggccaccg cactggcgcc gccgaacacc gccgcaagct tgtctgccca
     7021 cccggcccaa tgtgtccagc aatcgatggt ctcggcgacc tccgcctccg ccgcttcccg
     7081 gtcggtgcct ccgaggcaca actcctcgac cagatgtgag cggtgaccgt cgagcgcctc
     7141 tgccacctcg aagagcacct ggcctcgccg ttgcggtgcc agcgctgccc actccgagcg
     7201 tgcgtcgatg gcggccgcga ccgcggcgga aacctcggcc gtgtccgcct cgcatatccc
     7261 tgccgtcgta ccgtcggcga gggtcacctc cgcccaacgc cccgacatcg attcggccat
     7321 ggcaccgccg atgagatgcc ggtaacgacg tcgagacgtc gtcgacatcg gggaggcttt
     7381 ctgcggatcg accgaactca tggcgtctct cccccgtcgc cgtgtcgagg ccgagacccg
     7441 tcgattgcgc tggcgccctc gcaccggccg cgatcggcgg acaggtcgag ataggcaccg
     7501 accccgaacc gccctccacg gtcccctccc agcgaccggg cgagtcccca agtcggactc
     7561 gcagggtcga ccaggtcgta ggaattcacc cacacaaacc cggcgtgcag ctgatcggcc
     7621 acccactgtg agcgggcagc gtcggtggtc cacaccccgg ccgaccgccc cgacgccacc
     7681 tgattcgcaa tcgctacggc ctcgtcgtga gtccgaaatg tcaacacgct gagcaccgga
     7741 ccgcgggtca cctcagccag cgagcgatga ggcaattcaa ctcggtgcaa gagggtcggt
     7801 ttgacgaacc atccgcgctc gggcggccgc ttggcccagc gaacgacctc agccccatct
     7861 tgcctcgctg ccgccaccag agcctcagtc cgacccacct gttcggccga gtcgagcggg
     7921 ccgacgtcgg tgttgttgtc gagcgggtct cctacccgta cctgctgcag tctccgggcg
     7981 agacggtcga ccacctcttc tgcgatcgac tcctcgacga gcactcggct cgcgacatcc
     8041 catggctcgc ttcgatgttg ccaaattcca cgcacgaccg cgtcgatcgc ctgatcgagc
     8101 ggcgcgtcgt caaagatcag ctgcgcggag ttgccttcca gttccaacaa caaccgggtg
     8161 ccagccttcg ccgtcgattg tgcgacggct ttgccggctt cagccgaacc ggtgaactcc
     8221 agggccccca gcgccgggtg agcagcgagt gcggcccccg tgtcgtcgcc gccggtgacc
     8281 acgttgacca cccctggagg caaccttgcc tcgcggcgga tgtgagcgaa catcaacgcg
     8341 ctcagcgacg cgcgttgaga tggcttcacc acgacg
//
LOCUS       DAOOMA010000034         8060 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_10494_length_8060_cov_3.950031, whole genome shotgun sequence.
ACCESSION   DAOOMA010000034 DAOOMA010000000
VERSION     DAOOMA010000034.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 8060)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8060)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8060
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10494_length_8060_cov_3.950031"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..347)
                     /locus_tag="PLP95_01845"
     CDS             complement(<1..347)
                     /locus_tag="PLP95_01845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB44573.1"
                     /translation="MLDHGWWPVVVGLCGALIGTGALSDNSFLTQLATGRLILDGAVP
                     THDLYTWASEGTPLVVPAWGASGLYALADRVGSGLGVRLIIAACTAALILALWRLSAP
                     AKANVARLGLVSVA"
     gene            complement(452..1393)
                     /locus_tag="PLP95_01850"
     CDS             complement(452..1393)
                     /locus_tag="PLP95_01850"
                     /inference="COORDINATES: protein motif:HMM:NF012694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HPB44574.1"
                     /translation="MSGALLVPLILVMVAVAGVVFAIASGAHDRQVVRESLRSLDGYD
                     LADGAPMPNMRADALAESLSKRAIVPVMAKAVGLGRRFTPIGYMAKVRQRFTAAGITT
                     PDAVDRFIAVKTVMTPVGIALGLGNLVFGWVLSGMMGLVASLVIGGGLAYGPDLWLNR
                     KVEDRQSAVMKTLPDVLDLLTISVEAGLGFEQALDRVIASVPGPLSDEFARMLGESRS
                     GASRADAMRAMDERVQVPELRSFVLAIVQADAFGVSIGRVLRAQSEEMRIKRRQLAQE
                     KAQKAPVKMLIPMVFCIFPALFCVVLGPAALNIKENF"
     gene            complement(1396..3324)
                     /locus_tag="PLP95_01855"
     CDS             complement(1396..3324)
                     /locus_tag="PLP95_01855"
                     /inference="COORDINATES: protein motif:HMM:NF012694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HPB44575.1"
                     /translation="MRRSVSSVLVSLVLGVTAVAGGATPSGAEVNDETPPLQIVAVDA
                     RGGQLSADYLSAGEPVDEVTLEVDGATYRQPAEPLSQFGVRPSVMVVLDNSAGVSNAT
                     LRVAMEDLAALAPSEGGIAQLGVVTTGNGAALIGRPSTSFDGASANFGGVRVGGTSSL
                     WDATSIAATSHGSAPNLRRHVVLVVGSTDDASAASFSQVVRTLQDNDTVVHVVGVSGS
                     TADGAALRDLVRSTGGTFQTGSDADFAAIFDRIAATIDGERRVTIDSVPDSSAEFLSI
                     RLTVGDDTVTGGVRPGVFASGATAVTATPVQDGAGSGFFTSDAVKYLIIVFGAVAAGA
                     AVFAVAMLITKRRDNLNYALRYYDESYDAPRFAEEEDATAHATAALLQRAVAVTETIA
                     KERGLLHRVEAKLEGADLPLRPAEALTFYLTGAAAAILGAAVLTRSMMTVLVVLIVAM
                     VLPGFSVDFMAKRRKKKFTAMLPDMLTLLAGTLRAGYSIGQGIEAVSNEIDDPMGKEL
                     RRAVTEARLGRPLDEALEGIAQRLDSEDFGWAVMAIRIQREVGGNLAELLMTVADTMV
                     QRERLRRDVAALTAEGRMSAIILGLLPPGLAVVMYVMNPEYISKLFSGMGLYLLVGAV
                     VMMIIGFVWMKKTITIEV"
     gene            complement(3345..4637)
                     /locus_tag="PLP95_01860"
     CDS             complement(3345..4637)
                     /locus_tag="PLP95_01860"
                     /inference="COORDINATES: protein motif:HMM:NF012652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CpaF family protein"
                     /protein_id="HPB44576.1"
                     /translation="MDELRQRIHSHLIEELGPILYDNRLSGDELRRRVQEQLLAILGQ
                     ERAPLSAADKAQLIQEVSDDILGYGPIEKLLGDENVSEVMVNGPDFVYVERGGKVERD
                     GTTFVDADHLRRVIDKIVSAVGRRVDESTPMVDARLPDGSRVNAVIPPLAIGGPFLTI
                     RKFSKDPLQVDDLIRFGSLNAASARFLQACVVGKLNVIVSGGTGSGKTTLLNVLSSFI
                     PSNERIVTIEDAKELQLQQDHVLCLESRPPNVEGRGEVSIRDLVKNSLRMRPDRIVIG
                     ECRSGEALDMLQAMNTGHDGSLTTLHSNSPRDALARLETLVLMSGYDLPVRAIREQTA
                     GAVDMVVQLSRLRDGSRRVTHITEVQGMEGDVITLQDVFLFDFAAGVDEHGKFRGQLK
                     ATGVRPRFVEKLADQGIRLGPEVFSPGGDGSRPGMGRR"
     gene            complement(4855..6285)
                     /locus_tag="PLP95_01865"
     CDS             complement(4855..6285)
                     /locus_tag="PLP95_01865"
                     /inference="COORDINATES: protein motif:HMM:NF013793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-loop NTPase"
                     /protein_id="HPB44577.1"
                     /translation="MSDHTPHDEHGFGYGTSTDQYGADQYGADQYGADQYGAEFSDAT
                     YGGTSYGEASYGEPEWATATPPPPTGSPAIASGDVAAPTSPSIRVSVAVVESNPQMRD
                     VILAQLGGGATPFSSIEELTSRLTGSVPVVIVLGPSCSAPATLALTDHLLATNATLGA
                     ILVTDELTTSLLQQAMRIGVKDVLTLQGDIDQLSAAIQRVAVNLDAAQQRVQLGGPSD
                     SVEEFGEGSGELGEVITVFSTKGGAGKSVLATNLAVALAQRSAGPVCLVDADLQFGDV
                     AVMLKLTPHHTIVDAVSSLDRMDSALLDSLLVTHEASGLKVLPAPLEPAFADQVGAAE
                     MVAIVEQLRKFCSYVIVDTPAYFNEVVLGLVEVSDKVVLVAGMDIPNIKNVKIGLQTL
                     RLLNTPMDKLMLVLNRSNSKVKLDIGEVERTLQIKADALIPSDVLIPQSVNKGVPVIL
                     HAPRSPVAKSIQQMAELFAPVGAKRR"
     gene            complement(6290..7147)
                     /gene="cpaB"
                     /locus_tag="PLP95_01870"
     CDS             complement(6290..7147)
                     /gene="cpaB"
                     /locus_tag="PLP95_01870"
                     /inference="COORDINATES: protein motif:HMM:TIGR03177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp pilus assembly protein CpaB"
                     /protein_id="HPB44578.1"
                     /translation="MSSRRTLILIGALVAGLLAAFMTSKYVGGIEDRAQGDVQLTSVV
                     IARGGIGRGSNADEAIAAGAIDVGERRQVDVPADAILRPAEIKGMLAQIDLQPGTIIT
                     ASMFSNDTVAQNGATAAIAEGMTFQTISFDEVRGVAQLPQPGDYVNITLSGQCTPGTT
                     DFVIGAASEEGGAAPAMESCTASLFQKARIAAVGRSLGSAVAAPTDPAAPETTVPPTS
                     NMITFELPPAAAQVLVAASSSANIQIWLSLVRKDYVPEPIEATRVIPAAGVLGVTPYG
                     TATDSNENG"
     gene            complement(7366..>8060)
                     /locus_tag="PLP95_01875"
     CDS             complement(7366..>8060)
                     /locus_tag="PLP95_01875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018226090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HPB44579.1"
                     /translation="PGERRHGVVSSVVNFGAFVDLGGMDGLVHVSELSWKHVDHPGSV
                     VQVGDEIEVEVLEVDMSRERISLSLKATQQDPWQEFASNHQVGELVYGRVTKLVPFGA
                     FIQVGDGIEGLVHISEMSSHHVDLPEQVVTPGEELWVKIIDLDLQRRRISLSIKQAAE
                     GGIVAAEYQEHFGEHAYDDEGNYIGGAIEGTSEGQEAWEQYYAEQGFAADGVTPLEGF
                     EAPAAEGTSEEA"
BASE COUNT         1483 a         2672 c         2594 g         1311 t
ORIGIN      
        1 gcgaccgaga cgaggccgag gcgggcgacg ttcgcctttg ccggcgcgct cagacgccac
       61 aacgccagga tcagcgcggc cgtgcacgca gcgatgatca gccgaactcc gaggcccgag
      121 ccgacccgat cggccaacgc gtacaacccg gaggctcccc aggccggaac caccagcggt
      181 gttccctccg acgcccacgt gtagagatca tgggtcggca cggctccgtc gaggatcaaa
      241 cgcccggtcg ccagttgggt gagaaagctg ttgtcgctca gcgctccggt gccgatcaac
      301 gccccgcata ggccgaccac gaccggccac cagccgtgat cgagcaatga gaatccgccc
      361 ggccgcccgc cacaattctc gttctccgat aatgagtggt cgtgatcacg accactcatt
      421 atcggagaac gggtggcgag ccgcgagggc actagaagtt ctctttgatg ttcagcgcgg
      481 ccgggccgag gaccacgcag aacagcgcag ggaagatgca aaacaccatc gggatgagca
      541 tcttcaccgg ggccttctgc gccttttcct gagcgagctg acggcgcttg atgcgcatct
      601 cctccgactg ggcgcgaagc acgcggccga tcgacacgcc gaaggcatcg gcctgcacga
      661 tcgccaacac gaacgaacga agctccggaa cctgcacgcg ttcatccatc gcacgcatgg
      721 catcggcccg cgacgcaccc gaccgggact cgccgagcat ccgggcgaac tcatccgaca
      781 gcggccccgg caccgacgcg atcacccgat cgagcgcttg ttcaaagccg aggccggcct
      841 cgaccgaaat cgtgagcagg tccaacacgt ccggcagggt cttcatcacc gcggactgac
      901 ggtcctcgac cttgcggttg agccacaggt cggggccgta ggccaacccg ccgccgatca
      961 ccaacgaggc gaccaacccc atcatcccgg acaggaccca gccgaacacc agattgccaa
     1021 gcccgagcgc aatgcccacc ggtgtcatca ccgtcttgac agcgatgaac cggtccacgg
     1081 cgtcgggggt cgtgataccc gcagcggtga agcgctggcg caccttggcc atgtacccga
     1141 tcggagtgaa acgccggccg aggccgaccg ccttggccat caccggaacg atcgcccgct
     1201 tcgacaacga ctcggcgagg gcgtcggccc gcatgttcgg catcggagcg ccgtccgcga
     1261 ggtcgtagcc gtcgaggctg cgcaacgact cgcgaaccac ctgacgatcg tgggcaccgg
     1321 acgctatggc gaacacgacg ccagcgaccg ccaccatgac caggatgagg ggaaccaaca
     1381 aagcaccaga catggtcaaa cctcgatcgt gatcgtcttt ttcatccaca cgaagccgat
     1441 gatcatcatc accacggctc cgacgaggag atacaggccc atcccgctga acagcttcga
     1501 gatgtattcg gggttcatga cgtacatgac gaccgccagc ccgggaggaa gtaagccgag
     1561 aatgatcgcc gacatacggc cctcggcggt gagtgcagcc acatcgcgac gcagtcgctc
     1621 gcgttgcacc atggtgtcgg cgaccgtcat caacagttcg gcaaggttgc cgccgacctc
     1681 gcgctggatg cggatggcca tcaccgccca gccgaagtcc tcgctgtcga gtcgctgggc
     1741 gatcccttcg agcgcctcgt cgaggggtcg tccaaggcgg gcttcggtca cggcccggcg
     1801 aagctccttg cccatcggat cgtcaatctc attcgacacc gcctcgatgc cctggccgat
     1861 cgaatatccg gcgcgaagcg tgcccgcgag cagcgtcaac atgtcgggca gcatcgcggt
     1921 gaacttcttc ttacggcgtt tggccatgaa gtcgacgctg aacccaggaa gcaccatcgc
     1981 cacgatcaac accacgagca cggtcatcat gctgcgggtg agcacggccg caccgaggat
     2041 cgccgccgcc gctccggtga gatagaaggt cagggcttcg gcggggcgca acggaagatc
     2101 cgccccttcg agcttcgcct ccacccgatg caacaggccc cgttccttgg cgatcgtctc
     2161 ggtcaccgcc accgcccgct gcaacagcgc cgcggtggcg tgcgccgtgg catcctcctc
     2221 ctcggcgaag cgcggcgcat cgtaggactc gtcgtagtac cgcagcgcgt aattgaggtt
     2281 gtcgcgacgc ttggtgatca acatcgctac ggcgaacacg gcggcaccag ccgccaccgc
     2341 accgaagacg atgatcagat acttcaccgc atccgaggtg aagaacccgc tgcctgcacc
     2401 atcctgcacc ggcgtggcgg tcaccgccgt cgcaccgctg gcgaagaccc cgggccgaac
     2461 gccgccggtg accgtgtcgt cgccaacggt caggcgaatc gacagaaact cagcggaaga
     2521 gtcgggaacc gagtcgatcg tgacacgacg ttcgccgtcg atggtcgcag cgatgcggtc
     2581 gaagatcgcg gcgaagtcgg cgtcggaacc cgtttggaac gtaccgccgg tcgaacggac
     2641 gaggtcgcgc aatgcggcac cgtcggcggt ggaaccggag acgccgacga cgtgcaccac
     2701 cgtgtcgttg tcctgcagcg tgcgaacgac ctgggagaac gaagccgcgc tcgcatcgtc
     2761 ggtcgatccg acaaccaaca ccacgtggcg acgcaggttc ggcgccgagc cgtgggaggt
     2821 cgccgcaatc gacgtggcat cccacaatga gctggttcct ccgaccctga ccccgccgaa
     2881 gttggccgac gccccgtcga agctggtcga cggacgtccg atcaatgcgg cgccgttgcc
     2941 ggtggtcacg acgccgagtt gggcaatccc gccctccgac ggagcgagtg ccgcgaggtc
     3001 ctccatcgcg acccgcaacg tggcattcga cactccggcg gagttgtcga gtaccaccat
     3061 cacgctcggg cggaccccga actgcgacag cggctctgcc ggctggcgat aggtggcgcc
     3121 gtcgacctcg agggtgacct cgtcgaccgg ttcacccgca gaaaggtaat ccgcgctcaa
     3181 ttgtccgccg cgagcgtcca ccgcgacgat ctgcaacggc ggcgtctcgt cgttgacctc
     3241 tgctcccgag ggggtggcac ccccggcgac cgcagtgacg ccgagcacca ggctcaccag
     3301 caccgaagaa acgctgcgtc gcatgcgacg acctcctgaa gtcctcatcg gcgccccatc
     3361 cccggacgcg aaccgtcgcc gccgggcgag aacacctctg gcccgagacg aatgccctgg
     3421 tcggcgagct tctcgacaaa cctcgggcga acacctgtcg ccttgagctg tccgcggaac
     3481 ttgccgtgct cgtcgacccc ggcggcgaag tcgaacaaga acacgtcctg gagggtgatg
     3541 acatcgcctt ccatgccctg gacctcggtg atgtgggtga cgcgtcgcga gccgtcgcga
     3601 agtcggctga gctgcaccac catgtcgacc gctccggccg tctgttcacg gatggcccgc
     3661 accgggaggt cgtacccgct catcagcacg agcgtctcga gacgggcgag cgcatcgcgg
     3721 ggcgagttgg agtgcaacgt ggtcagcgag ccgtcgtggc cggtgttcat cgcctgcaac
     3781 atgtcgaggg cctcaccaga acggcactcg ccgatgacga tgcgatcggg ccgcatacgc
     3841 agcgagttct tcaccaggtc gcgaatactc acctcgccgc gaccctcgac gttgggcgga
     3901 cgcgactcga ggcacagcac gtggtcctgt tgcagctgga gttccttcgc atcctcgatg
     3961 gtcacgatgc gctcgttgga cggaatgaac gaactcaaca cgttcagcag cgtcgtcttt
     4021 cccgaaccgg taccacccga cacgatcacg ttgagtttgc cgaccacaca ggcctgaagg
     4081 aaccgagccg atgccgcgtt gagcgatccg aagcggatga ggtcgtcgac ctgcagcgga
     4141 tccttcgaga acttacggat ggtgagaaac gggccgccga tcgccagggg cgggatgacg
     4201 gcgttcacac gggatccgtc gggaaggcgc gcatcgacca tcggggtcga ctcgtcgacg
     4261 cgacgaccga ccgcggacac gatcttgtcg atcacccggc ggaggtgatc ggcatccacg
     4321 aacgtagtgc catcgcgttc gaccttgccg ccgcgttcca cgtacacgaa gtcggggccg
     4381 ttcaccatga cttcggagac gttttcgtcg ccgagcagct tctcgatcgg gccgtaaccc
     4441 aaaatgtcgt cggacacctc ctggatgagc tgtgccttgt ccgccgccga cagcggggca
     4501 cgctcctgtc cgaggatggc cagcaactgt tcctgtaccc gccggcgcag ctcgtcaccc
     4561 gacagccggt tgtcatagag gatcgggccc agttcctcga tgaggtggct gtggatccgc
     4621 tggcgcaact catccatgat cgggtcacgc cggggggccg ccaccgcggt gccggcgcca
     4681 acaccggacg taccggccga tgatccgctg ccgccgtgtc cgcccgaggc cggggcgcct
     4741 gggtgcgcag gcggggtcgg tgaagcaccg gcaccttggt ttgcctcatg aagacgttgg
     4801 tacagcgaca cctggggaac tcctgatcaa gggcgagtgg gcgaaacggt ggggtcagcg
     4861 gcgtttggcg ccgacggggg cgaacagctc cgccatctgt tgaatggact tggccaccgg
     4921 agaccgcggg gcgtgcagga tgaccggcac ccccttgttg accgactggg ggatcaacac
     4981 gtcggaggga atcagagcat cggccttgat ctgaagcgtt cgctccacct cgccgatatc
     5041 gagtttcacc ttcgagttgg agcggttcag caccagcatc aacttgtcca tcggcgtgtt
     5101 gagcaagcgc agggtctgca ggccgatctt cacgttcttg atgttcggaa tgtccatgcc
     5161 ggccacgagc accaccttgt ccgacacctc gacgagaccg aggacgactt cgttgaagta
     5221 ggcgggggtg tcgacgatca catagctgca gaacttgcga agctgctcga cgatggcgac
     5281 catctcggcc gcccccacct ggtcggcgaa ggccggctcg agtggagcgg gcaacacctt
     5341 gagtcccgat gcctcgtggg tgacgagcaa cgagtcgagc aaggccgagt ccatccgatc
     5401 tagggaggac acggcatcga cgatggtgtg atgcggagtg agcttgagca tgaccgccac
     5461 atcgccgaac tgcagatccg cgtcgacgag acacaccggt ccggcggaac gctgggcgag
     5521 ggccacggcc aggttcgtgg cgagtaccga cttcccggcg ccacccttgg tggaaaacac
     5581 cgtgatgacc tcgccgagtt caccggaccc ttcgccgaat tcctcgaccg agtccgacgg
     5641 accgccgagt tgcacgcgct gttgggcggc gtcgaggttg accgcgaccc gctggatcgc
     5701 cgccgacagt tgatcgatgt cgccctgcag ggtcagcaca tccttgactc cgatgcgcat
     5761 cgcctgttgc aacagggagg tggtgagttc gtcggtgacc agaatcgccc cgagcgtggc
     5821 gttggttgcg agcaggtgat cggtcagcgc cagcgtggcc ggcgccgaac atgacggacc
     5881 gaggacgatg acgaccggga ccgaaccggt gaggcgactc gtgagttcct cgatcgagct
     5941 gaacggggtc gctcctccac cgagctgagc gaggatgacg tcgcgcatct gcgggttcga
     6001 ctcaacgacc gcaaccgaaa ctcggatgga cggtgacgtc ggcgctgcga catcgccaga
     6061 agcgatcgcc ggcgagccgg tgggcggcgg tggggtcgcg gtcgcccact ccggttcacc
     6121 gtaggaggct tcaccgtagg aggtgccgcc gtaggtcgcg tcggagaatt ccgctccgta
     6181 ctggtcggct ccgtactggt cggcgccgta ctggtcggcg ccgtactggt cggtcgaggt
     6241 cccgtatccg aagccgtgct catcgtgagg tgtgtgatcg gacatgggat cagccgttct
     6301 cgttggagtc ggtggcggtg ccatacggcg tgacgcccaa cacaccggca gccgggatga
     6361 ctcgggtggc ttcaatcggc tccgggacgt agtccttgcg cacgaggctc aaccagatct
     6421 ggatgttcgc cgaactcgag gcggcaacca gcacctgtgc ggcggccgga ggcagttcga
     6481 aggtgatcat gttcgaggtc ggcggcacgg tcgtctcggg ggcggcggga tcggtcggcg
     6541 cagcaaccgc cgaacccagc gaacggccga cggcggcgat gcgtgccttc tggaacagcg
     6601 aagcggtgca ggattccatc gcaggagcgg cgccgccctc ctcgctcgct gcgccgatga
     6661 cgaagtcggt ggtgccgggc gtgcattggc cgctgagggt gatgttcacg tagtcgccgg
     6721 gctggggaag ctgggcgacc ccgcggacct cgtcgaagct gatcgtctga aaggtcatcc
     6781 cctcggcgat cgccgctgtc gcgccgttct gagcgacggt gtcgttgctg aacatcgatg
     6841 cggtgatgat ggtgccgggc tgcagatcga tctgggcgag cattcccttg atctccgccg
     6901 ggcgcaggat ggcgtcggcc ggaacgtcga cctgacgccg ttcgcccacg tcgatcgctc
     6961 ccgcggcgat ggcctcatcc gcattcgaac cgcggccgat accgcccctg gcgatcacca
     7021 ccgatgtcag ctgaacatcg ccctgtgccc gatcctcgat acctccgacg tatttcgagg
     7081 tcatgaaggc ggccagcaat cctgccacca gtgccccgat cagtatcagt gttctacgag
     7141 aactcacgac gacctcgatc cgttgtctgc gctacgtgcg gccgccggca ccccctgatg
     7201 aggtcccttc gcgacggctt gccacgtcat tcgtcggcag caacgacgcg attacttagg
     7261 atgtcgtgtc aaaaatccgt gctcaccgcc aaaacgacag cggcacccgg ccggaaccgg
     7321 atgccgctgc tcaattgatc gaacggtcag tcgaccgtgt ggggactacg cctcttcgga
     7381 agtgccctcg gcggccggag cctcgaatcc ctcgagcggg gtcacgccgt cggcggcgaa
     7441 tccctgctcg gcgtaatact gctcccaggc ttcctgacct tcggaggtgc cttcgatggc
     7501 gccaccgatg tagttgccct catcgtcata ggcgtgctcg ccgaagtgct cctggtattc
     7561 ggcggcgacg atgccaccct cggcagcctg cttgatcgac aggctgatcc ggcgacgctg
     7621 caggtcgaga tcgatgatct tgacccagag ttcctcgccg ggggtgacga cctgctcggg
     7681 gaggtcgacg tgatgcgacg acatctccga gatgtgaacg agaccctcga tcccgtcgcc
     7741 gacctggatg aacgcaccga agggcaccag cttggtgaca cggccataga ccagttcgcc
     7801 gacctggtgg ttggaggcga actcctgcca cggatcctgc tgggttgcct tgagcgacag
     7861 gctgatgcgc tcgcggctca tgtcgacctc gagcacctcg acctcgatct cgtcgccgac
     7921 ctggacgaca gaacccgggt gatcgacgtg cttccacgac agttcggaga cgtgcacgag
     7981 gccgtccatg ccgccaaggt cgacgaacgc accgaagttg acgacgctgg agaccacacc
     8041 gtggcgacgc tcgcccggct
//
LOCUS       DAOOMA010000035         7778 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_11038_length_7778_cov_3.990289, whole genome shotgun sequence.
ACCESSION   DAOOMA010000035 DAOOMA010000000
VERSION     DAOOMA010000035.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 7778)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7778)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7778
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11038_length_7778_cov_3.990289"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..631)
                     /locus_tag="PLP95_01880"
     CDS             complement(<1..631)
                     /locus_tag="PLP95_01880"
                     /inference="COORDINATES: protein motif:HMM:NF012766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HPB44580.1"
                     /translation="MADQPQTPDPVHRVGRERDERRRVERLIEAQSRASELFDIVEHE
                     GVIVAGITEAEASRRIAAIAADRFGVVNHWHKRIVRSGPNTLATYRADPPDRVIEADD
                     IVFGDFGPIFDGWEADFGRTWVIGDDPVKLRLRDDLEAIFDLGVAFANTRADVTGAEL
                     YGYVVGEALRRGWTFGNFHCGHLVGEFPHDDLESSHPHAHLAASNDAPLR"
     gene            complement(665..1546)
                     /locus_tag="PLP95_01885"
     CDS             complement(665..1546)
                     /locus_tag="PLP95_01885"
                     /inference="COORDINATES: protein motif:HMM:NF012517.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HPB44581.1"
                     /translation="MQISMTLPTMLPHGRREVVEWCHAVDEGPFSSLAVPERVTFPSH
                     HWVVELSAAAALTQRVRLWTTIVVLPAHDEVDVAKQLASIDRLCEGRLTVGVGVGGRD
                     HDYLAIGGSPRRKWQRLDEQVARMRSIWAGDPPFEGADPVGPPPVQFGGPPLVSGSMG
                     PKALARAARWADGIDDASTIFRVDPDATAAAFERIRTAWTDAGRSDRPHISVSVWYAL
                     GDGSQERLRDYAYRYLRIMGEGFAKSCAGLVECYTPEALRGAVATLRDLGCDELFLVP
                     TTADVTELDRTREALGM"
     gene            complement(1800..2300)
                     /locus_tag="PLP95_01890"
     CDS             complement(1800..2300)
                     /locus_tag="PLP95_01890"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HPB44582.1"
                     /translation="MPEYSIRELTSADLASATVINNANVPAVGAADEAKMAHLCELAE
                     VALAVVDANDVVVGFCVVLPAGTAYESVNYRWFGERFDDFVYLDRVAFAASACNQGLG
                     TRLYGEVERRVSAAQFCCEVNLRPRNDGSLRFHERLGFAEVGQLETDYGVLVSLLAKP
                     LRSAGE"
     gene            2430..2813
                     /gene="ssrA"
                     /locus_tag="PLP95_01895"
     tmRNA           2430..2813
                     /gene="ssrA"
                     /locus_tag="PLP95_01895"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            3341..3511
                     /locus_tag="PLP95_01900"
     CDS             3341..3511
                     /locus_tag="PLP95_01900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB44583.1"
                     /translation="MNASALFAELCEVIDRSAAQFNLDTFAELIDEVGRQRRDDVDEF
                     ADGGVVELVGFP"
     gene            complement(3671..3937)
                     /locus_tag="PLP95_01905"
     CDS             complement(3671..3937)
                     /locus_tag="PLP95_01905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB44584.1"
                     /translation="MPLAWQLDTGIDVPDVANLVFFKIVRSATKFWQMVGRGTRLRLD
                     LFGPGQDKGSFAIFDFCQNLEYFGQNPGGHEGGAGVPLSTRLFA"
     gene            3977..4555
                     /locus_tag="PLP95_01910"
     CDS             3977..4555
                     /locus_tag="PLP95_01910"
                     /inference="COORDINATES: protein motif:HMM:TIGR03083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03086 family metal-binding protein"
                     /protein_id="HPB44585.1"
                     /translation="MTNPSADLYERGLDRFEEVAQQLSTEQWDRASACEGWTNLDVLG
                     HLGNSVRMGAAYLRGEQPVAPAFDRPADLVSGAPMDFWRPLAADAREALAGADLDLEM
                     DTPMGRRTVADRLAFPAMDLWVHAWDIGHPIGIDLEIPAEVDAFAHRYIDPFPPEMVR
                     GPQGAFGPEVEVPADATATERFVAWTGRDPRA"
     gene            complement(4605..5042)
                     /locus_tag="PLP95_01915"
     CDS             complement(4605..5042)
                     /locus_tag="PLP95_01915"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HPB44586.1"
                     /translation="MIPSPGETGAQWWSNTGWETARRSQAERDGRATDGELDPVEEFL
                     HDVDQEIIDEARNHVADQSDAPFQTPWPLEAWPSIPTRFVLPTRDRFFPADFQRGIVR
                     ERLGVTPDEIACGHLPALARPVELASLLLDYERTQGATGSSRS"
     gene            5509..6081
                     /locus_tag="PLP95_01920"
     CDS             5509..6081
                     /locus_tag="PLP95_01920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB44587.1"
                     /translation="MALFIAGGVACSNDDATDSTTPTAADVATETDMTSDDSTTTAAS
                     GEADVIDVTAADFAFAGIPDTVPAGSRFSLTTAAGAEPHEMVVMKVPDGETRSIEDLV
                     ALPMEELEAIFAGDPVMVLISMPGTTDTPGVVFPEGAQPTVTEPGRYIVICSFPEGTT
                     PEDIENAQGPLEGPGTPHFMLGMTGEFTVE"
     gene            6301..>7778
                     /locus_tag="PLP95_01925"
     CDS             6301..>7778
                     /locus_tag="PLP95_01925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015103 - inorganic anion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HPB44588.1"
                     /translation="MTTAPLVSPDPAPPLLVMLRERGRLRIELLSGLVVALALIPEAI
                     SFSVLAGVDPRVGLFASFTMAVTIAITGGRPAMISAATGAVALVVAPIVERHGLDYLV
                     ATVILAGLIQIALSLAGAAKMMRFMPRSVMVGFVNALAILIFLAQIPELTDVPWAVYP
                     LAAAAIAVLVGFPRLTNAVPAPLVVIVSLTALVVAFGIGVPTVADRGRLPDSLPTLGL
                     PQVPFDLDTLRIIAPTALAMAIVGLLESLMTAKLVDDITDTHSDKTREARGQGIANVV
                     TGLFGGMGGCAMIGQTMINVKSGRARTRLSTFAAGTFLLVLVVGLGDIVGRIPMAALV
                     GVMVMVSVGTFDWHSIHPNTLRRMPASATAIMAVTVAVTVFTHNLAYGVGVGVLVAII
                     AFARRVAHFTSVEIAPTSNDDQRTYLVTGALFFASSNDLVYQFDYANDPDNVTIDLTN
                     AQIYDSSTVAALDAITVKYHDRGKRVEIIGMNEPSARWHELSG"
BASE COUNT         1385 a         2669 c         2415 g         1309 t
ORIGIN      
        1 accgcaacgg ggcgtcgttg ctcgcagcaa gatgcgcgtg gggatgcgac gattccaagt
       61 cgtcgtgggg aaactcgccg acgagatggc cacagtggaa gttgccgaac gtccagccac
      121 ggcgcagcgc ctcacccacg acgtatccat acagctccgc cccggtcacg tcggcacggg
      181 tgttggcgaa cgcgaccccc aggtcgaaga tcgcctcaag gtcgtctcgt aggcgcagct
      241 tgaccggatc atcaccgatg acccacgtgc gcccgaagtc ggcttcccac ccgtcgaaga
      301 tcggcccgaa gtcaccgaac acgatgtcgt cggcttcgat gacccggtcg ggcgggtccg
      361 cccggtaggt ggcgagagtg tttggccccg agcgaacgat gcgcttgtgc caatgattga
      421 cgaccccgaa ccgatcggca gcgatggcag cgatccgtcg actggcttcg gcttcggtga
      481 tgccggcaac gatcaccccc tcgtgctcga cgatgtcgaa caactccgac gctcgagact
      541 gggcctcgat gaggcgttcc acgcggcgac gctcgtcgcg ctcgcgtccg acccgatgga
      601 cggggtcggg ggtctggggt tgatcagcca tgtcgtgggt tgcctttcaa atgccgaggg
      661 cgtctcacat gccgagggct tcgcgggttc ggtcgagttc ggtcacatcc gccgtcgtcg
      721 gcaccaggaa gagctcgtcg cacccgaggt ctcgcagggt cgccacggcg ccacgaagcg
      781 cttctggcgt gtagcactcg accagaccgg cgcacgactt cgcaaacccc tcgcccatga
      841 tcctcaagta gcggtacgcg tagtcgcgaa gccgctcctg tgagccgtcg ccgagcgcat
      901 accaaacgct caccgagatg tgtggacgat cgctgcgtcc ggcgtcggtc cacgcggtac
      961 ggatgcgctc gaacgctgcg gccgtcgcat cggggtcgac tcgaaagatg gtcgatgcat
     1021 cgtcgatgcc gtccgcccat cgtgctgccc gggccagcgc cttggggccc atcgaccccg
     1081 acaccaatgg cggcccacca aattgaaccg gcgggggccc gaccggatct gcgccctcga
     1141 atggcggatc accggcccag atcgagcgca tgcgagccac ctgctcgtcc agtcgttgcc
     1201 acttgcggcg cggggaccct ccgatcgcca agtagtcgtg atcgcgaccg ccgacaccga
     1261 cgccgacggt gagacgcccc tcacacaatc gatcgatgga tgcgagttgc ttggcgacat
     1321 cgacctcgtc gtgtgcgggc aacaccacga tggtggtcca gagccgaacc cgttgcgtga
     1381 gcgcggcggc cgccgagagt tcgacgaccc agtggtggct cgggaaggtc acgcgctcgg
     1441 gcaccgccag actcgaaaat ggcccctcgt cgacggcgtg gcaccactcg accacctcac
     1501 ggcgaccgtg cggcagcatc gtcggcaacg tcatcgaaat ctgcatgtcc actcccaacg
     1561 agaccggcga gcaacgcgcc gacacccaag gtgccgtcga gcgttccttc gacccgagcg
     1621 accctacaac gggaccaccg ctcgtcagat actgctcgag gacggcgagg catcgctgcg
     1681 cggctcaacc tggtgaatcc gcaacggcaa cccggcaaat ccgcaactca agaccggcga
     1741 gccgacatgg agtggcacga gataccgaat tactccgtgg tgtcctcgac acactggacc
     1801 tactccccag ccgacctgag cggtttcgcg aggaggctca cgagcacgcc gtagtcggtc
     1861 tccaattggc cgacctcggc gaatcccagg cgctcatgaa accgcagcga cccgtcgttg
     1921 cgaggtcgca ggttcacctc gcaacagaac tgcgcagcgc tcacccgccg ttcgacctcg
     1981 ccatagagcc gcgtgcccag cccctggttg cacgctgatg ctgcgaacgc gacgcggtcc
     2041 aggtaaacga aatcatcgaa acgctcaccg aaccaccggt agttcacact ctcatatgcc
     2101 gtgccggctg gaagcaccac acagaagccg accacgacgt cgttcgcgtc gacgacggcg
     2161 agggccacct cggccagttc gcagaggtgc gccatcttgg cctcatcggc ggctccgacc
     2221 gcagggacgt tcgcattgtt gatgaccgtg gcgctcgcga gatcggcact tgtcagttcg
     2281 cggatcgagt actcaggcat ggtcggagcg tagggcgtca ccgcgcgagc cgaggtcgtg
     2341 acgcacgtcg cgacgacgac gctgagctgg ctcttcgaaa tgtttcgccg ggacgcgccg
     2401 tccaccgcta ccgtgaaggc cacaaacaag gggctgaagg gtttcgacgt tgggaccgga
     2461 agcttgttca tgcgacccga gttgctcaga ctcgttaaac agggcaacca aaagaagcgc
     2521 caatagcgac ttcgctctcg ccgcctgatc tgatcaggtc tagagagaca tcgtgagcag
     2581 caatgccgca cggggccaga tcgctacagc ccggcaacag aagtagcggt ggacagcagg
     2641 gagagaccgc tgacggtgag aaagaccact ggcatcgggg aaagacccgg cggtgaacct
     2701 cggttcaact gacccggcgg tgtggcagcc gtagagccac ggacacggga atggtcgtag
     2761 caggtcaagt gaccgccgag cggacgggag ttcgattctc cccagctcca cttcaagatc
     2821 cgcagtcatc ctccgatggc tgcggatccc ttgtttttcg aggttcaggg aggtgtgacc
     2881 gtccagccga gccggcggag gacctcggcc agggggcgag ttcaatgtgg ttggcttcaa
     2941 gaattggttg aagctcctcg aggatcatgc gctcgactgc tgttgccgtc atcagctttc
     3001 ccgtttcccg ctgacgtcct aacaaacacc tacgcgtcag atggtgccac gaacccctga
     3061 gcccggccga tcttctcggc gtccaggcgc gttcgccccc accgaacgtg gccgccggac
     3121 cgtcaggcag gtgcgtcgtc accagcgtgg cttccttgga gcgccttgca cgctcatccc
     3181 accgccgtcc ggtcgcggac ctcgtgcacc acggtgatga gccggtcgac gtccgccgcc
     3241 tcaaacaggc cgtcgggccc ttcgggcgcc acatcggtgt cgttgatggg ctggttcctg
     3301 cgcagtccct gcgtcggcga ggtagtcctc gtggtcgcgc atgaacgcga gcgccctttt
     3361 tgcggaactg tgcgaagtca tcgaccgatc cgccgcccag ttcaacctcg acaccttcgc
     3421 cgaactcatc gatgaagtcg gtcggcagcg tcgcgacgat gtcgacgagt tcgctgacgg
     3481 cggtgtcgtc gagctggtcg ggttcccata aagtgatctc gcggaagcgc tagatgagcc
     3541 gcagatgcgg gcgtacgacg aagttatcgg cgttcatcga cgccacgtcg ctgcgcagca
     3601 gctccgccaa ctctgcgcgt tcggtcgggt gggcgttgag cttgtcgagc gtttcgatca
     3661 gctcggcccg tcaggcgaat agtcgggtcg acagcggcac cccggcgccg ccttcgtggc
     3721 cgccggggtt ctggccgaag tactcgaggt tctgacagaa atcgaagatc gcgaagctgc
     3781 ctttgtcctg acccggcccg aacaggtcga gacgcaggcg ggtgcctcgc ccgaccatct
     3841 gccagaactt ggttgctgaa cgcacgatct tgaaaaacac cagattcgcg acgtcgggca
     3901 cgtcgatgcc ggtgtccaat tgccaggcca gcggcaacgt gcctgaacct cgccgacacg
     3961 cgcgagactg gccgctatga cgaatccatc agctgacctg tacgagcgcg gcctcgaccg
     4021 attcgaagag gtggcccaac agctgtcaac cgaacagtgg gaccgggctt cggcgtgcga
     4081 gggctggacg aacctcgacg tcctgggtca cctcgggaac tccgtccgga tgggtgccgc
     4141 ctacctccga ggcgagcagc cggtggcgcc tgcgttcgac cgtccagccg atctggtaag
     4201 cggcgcgccg atggattttt ggcgaccgct ggcggcggat gcccgcgagg cattggccgg
     4261 ggccgacctc gatctcgaga tggacacccc gatgggtcgg cgcacggtcg ccgatcgcct
     4321 tgccttcccg gccatggacc tgtgggtcca tgcctgggac attggccacc ccattgggat
     4381 cgacctcgaa atccccgccg aagtcgacgc gttcgcccat cgctacatcg acccctttcc
     4441 gcccgagatg gtgcgtggcc ctcagggcgc ctttggcccg gaggtggagg tacccgcaga
     4501 cgctacggcc accgagcggt tcgtggcctg gaccgggcgg gacccccgag cctgagtccg
     4561 catcgcgggt tgcctatcag tggcctgggg accagtaacg gccgtcacga acgggatgaa
     4621 cccgtcgcgc cctgggtgcg ttcgtagtcg agcaggaggg aagcgagctc gacgggtcgc
     4681 gcgagtgcag ggagatgccc gcaggcgatc tcgtcggggg tgactccaag acgttcgcgg
     4741 acgattccgc gttggaagtc ggccgggaag aagcggtctc gcgtcgggag cacgaacctg
     4801 gtcgggatgg acggccatgc ctcgaggggc cacggagtct ggaagggagc gtcagactga
     4861 tcggcgacat gattccgggc ttcgtcgatg atctcctggt caacgtcgtg gaggaactcc
     4921 tcgacagggt caagctcccc atcggtggcg cgaccgtcac gttctgcctg tgaccgacga
     4981 gcagtctccc acccggtgtt ggaccaccat tgggcgccgg tctcgcccgg cgacgggatc
     5041 atcgctgcga cgaggacgat caggcgagtt gtcgcaacac gtgctgcgac gagcggggct
     5101 tcacgagatg ccagtaccac gagtcggatc cggctccgtg aatcagggcg aaggtcgcca
     5161 tcaaaccctc tctctcgata gtcctcgctg cgcgatgcag gtctaccggc accgtagctg
     5221 aggttcacgc ggcggcgaac tcgctcaggg tcgggcgagc atgtactcat cacacgtcgc
     5281 ccttggtgtc gcgatccacc atcagcgccg cgtcaaccca tcgacggcca tgcccaacag
     5341 cgccagatcc gatcgatcca accgttgacg ccgccatgtc gtggtggaag ttttgcactg
     5401 agaggcctgg cacgcaggcg tctaacaggg ggaagagaag tgaagatgat gtacttaaat
     5461 atgaaccttc atcgtcgcgg catgcgccgg cttgtgccag tagcaatcgt ggcgttgttt
     5521 atcgctggtg gagtcgcgtg ctcaaacgac gatgcgaccg acagcaccac gcccaccgca
     5581 gcggacgtgg caaccgaaac cgacatgaca agcgacgact ccaccacgac cgccgcgtcc
     5641 ggcgaggccg acgtcatcga cgtgaccgcc gccgatttcg ccttcgcagg tattcccgac
     5701 accgtccctg cggggtcccg gttctcgctc accacggccg ccggcgcgga gccgcacgag
     5761 atggtcgtga tgaaggtgcc cgacggcgag acgaggtcca tcgaagatct cgtcgcactg
     5821 cccatggaag aactcgaggc aatcttcgcc ggcgacccgg tgatggtgct catttcgatg
     5881 ccgggcacga ccgacactcc aggagtcgtg ttccccgaag gagctcaacc aaccgtcacc
     5941 gagccgggcc gatacatcgt gatctgctca ttcccagagg gaaccacacc agaggacatc
     6001 gagaacgcgc aaggccccct cgaaggcccc ggcacacccc acttcatgtt gggcatgacc
     6061 ggcgagttca ccgtcgaata gccacacggc tcggaatcaa cgtctcagcg gccgcgcgac
     6121 aaggagttgc gtctgcggct ggcgcgcgtc ggccggaaac gccggcccgt cttgatactc
     6181 acgcgagacg gcgtattgga cgtgcgctca cgagtcgccc ccgtactctt ccctcacgta
     6241 aaggtagact tggacgcttc gcccgcccgc ggcacatacc gccacccccg gagcctccag
     6301 atgaccactg ccccactcgt ctcccccgac ccagccccgc cgctgttggt catgttgcgt
     6361 gagcgggggc gcctccggat cgaactgctc tccggcctcg tcgtcgcact ggccctcatc
     6421 cccgaggcga tctcgttctc cgtcctcgcc ggggtcgacc cccgggtcgg gctcttcgca
     6481 tcgttcacca tggccgtcac gatcgcgatc accggcggtc gaccggcgat gatctcggcc
     6541 gccaccgggg ccgtcgccct cgtcgtcgcc ccgatcgtcg agcgccacgg tctcgactac
     6601 ctcgtcgcga ccgtcatcct cgccggcctg atccagatcg ccctcagcct cgccggggcg
     6661 gcaaagatga tgcggttcat gcctcgcagc gtgatggtcg gcttcgtcaa cgctctggcg
     6721 atcctgatct tcctcgcaca gatccccgaa ctcaccgacg tgccctgggc tgtctacccg
     6781 ctggccgccg cagccatcgc ggtgctcgtc ggcttcccca gactcaccaa tgccgtgccc
     6841 gcaccgctcg tcgtcatcgt gtcgctcacc gcgctcgtcg tcgccttcgg aatcggtgtg
     6901 ccgaccgtcg ccgatcgcgg ccgtctgccc gacagtctcc ccaccctcgg tctgccacag
     6961 gtccccttcg atctcgacac cctgcgcatc atcgccccca cggccctcgc gatggccatc
     7021 gtcggcctcc tcgagtcgct catgaccgcc aagctcgtcg acgacatcac cgacacccac
     7081 tccgacaaga cccgcgaggc ccgtggccag ggcatcgcga atgtcgtcac cgggctgttc
     7141 ggcggcatgg gcggctgcgc catgatcggc cagaccatga tcaacgtgaa atcagggcgg
     7201 gcccgcacaa ggctgtcgac cttcgcggcc ggaacgttcc tcctcgtgct cgtcgtcggc
     7261 ctcggcgata tcgtcgggcg catcccgatg gcggccctcg tcggcgtcat ggtcatggtc
     7321 tcggtcggca cgttcgactg gcacagcatc cacccgaaca ccctgcgccg gatgccggcc
     7381 agcgccaccg ccatcatggc cgtcaccgtc gccgtcaccg tgttcaccca caacctggcc
     7441 tacggcgtgg gggtcggcgt cctcgtcgcc atcatcgcgt tcgcccggcg tgtcgcgcac
     7501 ttcaccagcg tcgagatcgc ccccacctcc aacgacgacc aacgcactta cctcgtcacc
     7561 ggggcgctgt tcttcgcgtc gagcaacgac ctcgtgtacc agttcgacta cgcgaacgac
     7621 cccgacaacg tcaccatcga cctgaccaac gcacagatct acgactcgtc gaccgtcgcc
     7681 gcactcgacg ccatcaccgt caagtaccac gaccgcggca agcgggtcga gatcatcgga
     7741 atgaacgaac cgagcgcccg ctggcacgaa ctcagcgg
//
LOCUS       DAOOMA010000036         7646 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_11308_length_7646_cov_4.456857, whole genome shotgun sequence.
ACCESSION   DAOOMA010000036 DAOOMA010000000
VERSION     DAOOMA010000036.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 7646)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7646)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7646
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11308_length_7646_cov_4.456857"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(129..917)
                     /locus_tag="PLP95_01930"
     CDS             complement(129..917)
                     /locus_tag="PLP95_01930"
                     /inference="COORDINATES: protein motif:HMM:NF025008.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HPB44589.1"
                     /translation="MTDALFRHHGGGRYEPTGSARGAWDPGIVHGAAVAALVVGRLGT
                     RSGTISRFTIDFLAPVPFEELTLELDEPIGGRRVQRRRARLLHGDRTVAEALALIVAT
                     NDLELPDKALRHHTPFDPDAVPDLCEPNHGAREVIGHDCFDSESVIVQRMRVEGDPRI
                     HQWIGLAGDVVDDEALQPIEVATVAADYAQGAVHRRLPFGDWSYRNAEQTLHFARAPR
                     SNWIGSRCDFVGSNLGAGYNSADLFDEAGRFGQAAGALVIETRG"
     gene            complement(941..2191)
                     /locus_tag="PLP95_01935"
     CDS             complement(941..2191)
                     /locus_tag="PLP95_01935"
                     /inference="COORDINATES: protein motif:HMM:NF012296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HPB44590.1"
                     /translation="MSTETTASVDISSTEFWQLSEVDRDKRFAALRATEGLTQHRPIE
                     LGGITDGPGFWAIVRRDDIQYVSRHAELFCSSKGVGFTDIPQEYNEAFGSFLMTDAPR
                     HTHLRGLISRAFTPKRVRTIEDQIQRQAVTIVGAAVDQGSVELVADLSMQLPLWTISE
                     MLGVPEDRRRELWDGANALVNAQARADAGDDEADALAEALMAGISLSALGVEFANARR
                     SDPRDDLLTSLVEAEVDGQRLTDEEIGAFIVLLGVAGNDTTRNTITLNVKAFADNPDQ
                     WDLLRSDPERYMAGAVEEMLRWATPVMTFRRTATRDTLVAGTEIREGDRLVMFYASGD
                     RDEAHFADPWRFDITRTPNEHVAFGGGGVHYCLGASLARTQLRCVFGELAKRVERFEV
                     GDPHMITSAFVNGVESVACTFVTA"
     gene            complement(2211..3395)
                     /locus_tag="PLP95_01940"
     CDS             complement(2211..3395)
                     /locus_tag="PLP95_01940"
                     /inference="COORDINATES: protein motif:HMM:NF016777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HPB44591.1"
                     /translation="MYLPIFDADNHLYETREAFTKYLPKEYRNVIRYVEVDGRTKIVV
                     NGTISEYIPNPTFDVVARPGAQEDYYRKGNPEGLSMREIYGKPIPAPEAFRSPEARLA
                     LMDEQNLERTLMFPTLASLLEERMRDYPEITHAVIHAFNQWLYETWQFNYQDRIYTTP
                     VITLPIVDKAIEELEWVVEHGAKAILIRPAPVPTYGGLSRSMALPEFDPFWAKVVEHD
                     LLVGLHSSDSGYDRYTNDWTGQGGEFLPFQPNPFRTFAMWRPIEDTVASLVCHGALSR
                     FPELKIAIIENGASWVAPLLAKMAELWKKQPQAFAENPVEVVKRNIHVSPFWEEDLGE
                     MAELLGEDRVLFGSDFPHPEGLESPRSYVEELSHLPEALQAKIMGGNLSRLMGVPAEL
                     PA"
     gene            3535..4749
                     /locus_tag="PLP95_01945"
     CDS             3535..4749
                     /locus_tag="PLP95_01945"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF014564.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HPB44592.1"
                     /translation="MTAILQGVRILEVAEHTFVPAASAVLSDLGADVIKIEHVERGDA
                     MRGLGSTGLAGLGGEVHPLLEHSNRGKRSIGLDLASDAGRDVLYRLAATCDVFLTNKM
                     PGVRSRLKIGVEEIRAANPDIIYARGTGQGEYGPDADKGSYDSLAFWARSGAAVGAMR
                     PEYDIVPPAPAPGFGDSVGGMTIAGGIMGALFHRERTGEATEVDVSLLGAGLWAMGQA
                     LALSDRLGMPWKPGPADQSMNPLVANYETSDGRWIALTCLQAGVYWGSMCEVIERLDL
                     AVDERFARHADLVANCTPAMHELSAAFAARPLAEWIERLANFTGQWAVVQDSVEVISD
                     PQTVANGYLQECYSAEGTAFRLVAAPVQYNGSPAVPTRAPQFNEHCEEILGEIGLDMD
                     AIIQLKIDGVVA"
     gene            4746..5774
                     /locus_tag="PLP95_01950"
     CDS             4746..5774
                     /locus_tag="PLP95_01950"
                     /inference="COORDINATES: protein motif:HMM:NF012296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HPB44593.1"
                     /translation="MTTDRVDGLNDDFYYDPFDREIDKNPYPIWKRLRDEAPWWYNER
                     KDFYTLSRFADVEEVMRDWDTYRSGHGSTLDIIKANIELPPGIILMEDPPEHDVHRGL
                     LSRVFTPRKMNALEPKIREFCARTLDPLRDAGSFDFVADLGAEMPMRTIGMLLGIPEA
                     DQVEIRDSLDEAMTYIMLLAGAGNETATRLIGWTGKLLAEHPDQRREIANDRSLIPNA
                     IEEVLRFEAPSPVQARVNYREVTWHGVTIPERSAVVILNGSANRDEREFVDGETFNIH
                     CDISRHLSFGYGIHFCLGAALARLEGRVALDEVLNRWPEWDVDWDNAKQAHTPHVRGW
                     ERLPVVTG"
     gene            5918..6556
                     /locus_tag="PLP95_01955"
     CDS             5918..6556
                     /locus_tag="PLP95_01955"
                     /inference="COORDINATES: protein motif:HMM:NF025840.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4433 domain-containing protein"
                     /protein_id="HPB44594.1"
                     /translation="MTTPQNGLLFHFTHIENLPSIIANGRRADSAVQTADALKVEVGE
                     DRIKKARRSRPVPIPPGGVVADYVPFYFAARSPMLYTIHCGNVASYQGGQDPIVYLVS
                     EISTVVDGGCTAVFTDRNAYYPYATFDADARRAYDEVDWPLMRASMWNNTGNEPDRME
                     RRMAEMLVHDAVPFSLVVEIAARSEAMLAQVNDLLASVEHRPALAVRTNWYF"
     gene            6566..7618
                     /locus_tag="PLP95_01960"
     CDS             6566..7618
                     /locus_tag="PLP95_01960"
                     /inference="COORDINATES: protein motif:HMM:NF013798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macro domain-containing protein"
                     /protein_id="HPB44595.1"
                     /translation="MIREAHGDLLSADVDAIVNTVNTVGVMGKGIALQFKKRFPDNFK
                     AYSAACKLGDVKLGEMFVFDAGQLVTPRWVINFPTKQHWKSRSRLKDIDAGLDDLVHV
                     IGELGIQSIALPPLGCGLGGLDWLEVRPLIERKLASVDADIVVYPPEGAPPAAAIVPS
                     GNRPTMSAGKAALVHIIDRYSPVAMGAGLIEIQKLMYFLQVAGQPLNLHYVKGIYGPY
                     ADNLRHVLNRVEGHFLTGFGDGSASVTEAEPIALLDGAGAEAEVVVAADDQLRRRIDR
                     VLALADGFESSYGMELLATAHWAASSEGCDDRACVIATVHGWSKRKEQLFTERHVVAA
                     LDQLVAHGWLGEDFAA"
BASE COUNT         1310 a         2298 c         2637 g         1401 t
ORIGIN      
        1 cggccgcgcc ggcgacctgg tggtgtgcgg cgtgggccgc gaatggctgc tcagccccat
       61 tccacaacct ccgccgatcg aggccgtggt catcggcggc accgtggtgc acgggaggct
      121 ggagcgactc agccgcgggt ttcgatgacg agggcgcccg ccgcctgacc gaatcggccg
      181 gcctcgtcga acaggtcggc gctgttgtag ccggccccga ggttcgatcc gacgaagtcg
      241 catcgcgatc cgatccagtt gctgcgaggt gctcgggcga aatggagtgt ctgttccgcg
      301 tttcgatacg accagtcgcc gaagggcagc cggcgatgga ccgcaccctg ggcgtagtcg
      361 gcggccaccg tcgcgacttc gatgggttgg agtgcctcgt cgtcgacgac atcgccggca
      421 agcccgatcc actggtgtat ccgggggtcg ccctcgacgc gcatgcgctg cacgatcacc
      481 gactcgctgt cgaagcagtc gtggccgatc acctcgcgtg caccatggtt gggctcgcac
      541 aggtcgggaa ccgcatcggg gtcgaagggc gtgtggtgac gaagcgcctt gtcggggagt
      601 tcgaggtcgt tggtcgccac gatcaacgcg agcgcctcgg cgaccgtacg gtcaccgtga
      661 aggagtcgcg ctcggcggcg ttgcacccgc cgtccgccga tcggctcgtc gagttcgagg
      721 gtcaactcct cgaagggcac cggtgcgagg aagtcgatcg tgaaccgcga gatcgtccca
      781 ctgcgggtgc cgagtcgtcc gacgacaagc gctgcgaccg cggcgccgtg gacgatgccc
      841 gggtcccagg caccgcgggc cgagccggtg ggttcgtagc gtccgcctcc gtggtggcga
      901 aagagcgcgt cggtcatcgt tgcgggcctc gggggagacg tcaggcggtg acgaaggtgc
      961 aggcgacgga ttcgacaccg ttgacgaacg cgctggtgat catgtgcgga tcgccgacct
     1021 cgaagcgttc gacccgcttg gcgagttcgc cgaacacaca gcgcaactgg gtgcgcgcga
     1081 ggctcgcacc gaggcagtag tgcaccccac cgccgccgaa ggccacgtgc tcgttcgggg
     1141 tccgggtgat atcgaatcgc cacgggtccg cgaagtgcgc ctcgtcgcgg tcccccgacg
     1201 cgtagaacat cacgagacgg tcgccctcgc gtatctcggt tccggcgacg agggtgtcgc
     1261 gggtggccgt gcggcgaaag gtcatcaccg gggtcgccca acggagcatc tcttcgacgg
     1321 caccggccat ataccgctcg gggtcgctgc gcaacaggtc ccattggtcg gggttgtcgg
     1381 cgaaggcttt gacgttcagc gtgatggtgt ttctcgtcgt gtcgttgccg gcgaccccga
     1441 gcaacacgat gaacgcgccg atctcctcgt cggtcagacg ctgcccgtcg acctcggcct
     1501 cgacgagcga agtcaacaag tcgtcgcggg ggtcggatcg acgggcgttg gcgaattcta
     1561 cgccgagcgc actcaggctg attccggcca tcagcgcctc ggcgagggcg tcggcctcgt
     1621 cgtcgccggc gtctgctcgt gcctgggcat tgaccagcgc gttggctccg tcccacagct
     1681 cgcggcgccg atcttcggga acgccgagca tctccgagat cgtccacaac gggagctgca
     1741 tcgacagatc ggcgaccaac tcgacgctgc cctggtcgac cgcggcgccc acgatcgtga
     1801 cggcctggcg ctggatctga tcctcgatgg tgcgcacgcg cttgggggtg aacgctcggc
     1861 tgatgagtcc gcgcagatgc gtgtggcgcg gtgcgtcggt catgaggaac gaaccgaagg
     1921 cttcgttgta ttcctggggg atgtcggtga agccgactcc tttgctggag cagaacagct
     1981 cagcgtggcg gctgacgtac tggatgtcgt cgcgtcgaac gatcgcccag aacccggggc
     2041 cgtcggtgat gcctccgagt tcgatgggac gatgttgggt gaggccttcg gttgctcgca
     2101 gtgccgcgaa tcgcttgtcg cgatcgacct cgctgagttg ccagaactcg gtggaggaga
     2161 tgtcgacgga agcggtggtc tcggtgctca tggtgtcctg ttcgatcggg tcaggcgggc
     2221 aattcggcgg gtacgcccat gaggcgtgag aggtttccac ccatgatctt ggcttgcagc
     2281 gcctcgggga ggtgtgagag ttcctcgacg tagctgcgcg ggctttcaag tccttcggga
     2341 tgcgggaagt cggagccgaa caggacccga tcctcgccga ggagttcggc catctcgccg
     2401 aggtcttcct cccagaaggg gctgacgtgg atgtttcgct tcacgacctc gacggggttc
     2461 tccgcgaacg cctggggctg cttcttccac agttcggcca tcttggccaa cagcggtgcg
     2521 acccacgagg cgccgttttc gatgatggcg atcttcagtt cggggaagcg gctgagcgcc
     2581 ccgtgacaca cgagggaggc gacggtgtcc tcgatcggtc gccacatggc gaaggtgcgg
     2641 aacgggttgg gttggaacgg aaggaactcg ccgccctggc cggtccagtc gttggtgtag
     2701 cggtcatagc cgctgtcgga ggagtgcagg ccgacgagga ggtcgtgttc gacgaccttg
     2761 gcccagaacg ggtcgaactc cggtagcgcc atggagcgcg acagcccgcc gtaggtgggt
     2821 accggggcgg ggcggatcag gatcgctttg gcgccgtgtt cgacgaccca ctcgagctcc
     2881 tcgatcgctt tgtcgacgat cggcagcgtg atgaccggtg tggtgtagat gcggtcctgg
     2941 taattgaact gccaggtctc atacagccac tgattgaacg cgtggatcac ggcgtgggtg
     3001 atctccgggt agtcgcgcat gcgttcctcg aggaggctcg cgagcgtcgg gaacatgagt
     3061 gtgcgttcga ggttctgttc gtccatcaac gcgaggcgag cctcgggcga gcggaacgct
     3121 tcgggcgccg ggatcggctt gccgtagatc tcgcgcatcg acagcccctc ggggttgccc
     3181 ttgcggtagt agtcctcctg ggcgccgggt cgggcgacga cgtcgaaggt cgggttcggg
     3241 atgtactcgc tgatcgtgcc gttgaccacg atcttggtgc ggccgtcgac ctcgacgtag
     3301 cgaatgacgt tgcggtactc cttcgggagg tacttcgtga acgcctccct ggtctcgtac
     3361 aggtgattgt cggcgtcgaa gatcggtagg tacatcgaag gccccctcat cgtgtcccgg
     3421 tctgatcgga cgataactgg ttctgacgtc ggaatcaaaa atgtggtaga gctgtgtcgt
     3481 gcgatgaacg ccgaaggcca ccaccgacgg gggctgtgac agggggagac cgccatgacc
     3541 gcgattctgc aaggggttcg gattctcgaa gtcgccgagc acaccttcgt tccggccgcc
     3601 tcggcggtgc tgagcgacct cggcgccgac gtcatcaaga tcgaacacgt cgaacgcggc
     3661 gacgccatgc gcggccttgg ctcgaccggg ttggctggac tcggcggaga ggtgcacccg
     3721 ctgctggagc actcgaaccg gggcaaacgc agcatcgggc tcgatctggc gagcgacgcg
     3781 ggtcgcgacg tgttgtatcg cctcgcggcg acctgcgacg tgttcctcac caacaagatg
     3841 ccaggggtcc ggtcgcgact gaagatcggc gtggaggaga tccgggcagc gaaccccgac
     3901 atcatctacg cccgaggtac ggggcagggc gaatacgggc ccgacgccga caagggttcc
     3961 tacgattcgc tcgcgttctg ggcccgcagc ggtgctgcgg tcggggcgat gcggcccgag
     4021 tacgacatcg ttccgccagc tccggcaccg ggctttggcg actcggtcgg aggcatgacg
     4081 atcgctggcg ggatcatggg agcgttgttc catcgggaac gaaccggcga ggcgaccgag
     4141 gtggacgtgt cgttgctcgg ggccggcctc tgggcgatgg gccaggccct tgcgctgtcc
     4201 gaccgcctcg ggatgccgtg gaaacccggg ccggcggacc agtcgatgaa tccgctcgtc
     4261 gcgaactatg agacgagcga tggacggtgg atcgcgctga cgtgtctgca ggccggggtg
     4321 tattgggggt cgatgtgcga ggtcatcgaa cgcctcgacc ttgcggtcga cgagcgcttc
     4381 gcgcgacacg ccgacctggt cgccaactgc acgccggcga tgcacgaact ctcggcggcc
     4441 ttcgcggcgc gtccgcttgc cgagtggatc gaacggctgg ccaacttcac cggtcagtgg
     4501 gcggtggtgc aagacagcgt cgaggtgatc tccgaccctc agaccgtggc gaacgggtac
     4561 ctccaggagt gttacagcgc tgagggcacg gcctttcggc tcgtcgcggc accggtgcaa
     4621 tacaacgggt cacccgcggt gccgacacgc gcaccgcagt tcaacgaaca ctgtgaggaa
     4681 atcctcggag agatcgggct cgacatggac gcgatcatcc agttgaagat cgatggggtg
     4741 gtggcatgac gacggatcgc gtcgacggcc ttaacgacga cttctactac gacccgttcg
     4801 accgcgagat cgacaagaac ccgtatccga tctggaagcg cctgcgcgac gaggcgccgt
     4861 ggtggtacaa cgagcgcaag gacttctaca cgctgagccg cttcgcggac gtggaagagg
     4921 tcatgcgtga ctgggacacg tatcgcagcg gtcacgggtc gaccctcgac atcatcaagg
     4981 ccaacatcga gttgccgccg ggcatcatct tgatggagga cccacccgaa cacgacgtgc
     5041 accgcggcct gttgtcgcgg gtgttcactc cccgcaagat gaacgccctg gagccgaaga
     5101 tccgcgagtt ctgcgcgcgg accctcgacc cgctgcgcga cgcgggaagc ttcgacttcg
     5161 tcgcggatct cggtgccgag atgccgatgc gcacgatcgg gatgctgctc ggtattcccg
     5221 aggcggatca ggtggagatc cgcgactcac tcgacgaggc gatgacctac atcatgttgc
     5281 tggcgggggc aggcaatgag acggcgaccc ggctcatcgg ctggaccggc aagctgctcg
     5341 ctgagcaccc cgatcaacgt cgcgagatcg ccaatgatcg ttccctcatc ccgaacgcga
     5401 tcgaggaggt cctgcggttt gaggccccgt caccggtgca ggcgcgggtc aactatcgcg
     5461 aggtcacctg gcacggggtc acgattccgg aacgcagcgc cgtggtgatc ctgaacggtt
     5521 cggcgaatcg cgacgagcgt gagttcgtcg acggtgagac cttcaacatc cattgcgaca
     5581 tctcgcggca cctcagcttc gggtacggca ttcatttctg tctgggtgcc gcgctcgcca
     5641 ggcttgaggg ccgcgtcgcg ctcgacgagg tgttgaaccg ttggccggag tgggacgtcg
     5701 actgggacaa cgcgaagcag gcccacactc cgcacgtgcg cgggtgggag cgcctaccgg
     5761 tcgtgaccgg gtgagctctg gacacccctt tctgcaactc gccagctggt gtagggccga
     5821 gaggtgaggg acgaaccggc ttcggttctg ttgaacgggt ctgagcagat caaatggcga
     5881 tctctagagt ggggcggacg agatctagga atgcgacgtg accactccgc agaatgggct
     5941 gctctttcac ttcacgcaca tcgagaacct gccctcgatt attgcgaacg gccggcgggc
     6001 ggactcagct gtccagacgg ctgatgcgct caaggtggag gttggggaag accgcatcaa
     6061 gaaggccagg cgatcgcggc ccgttcccat cccgccgggt ggcgtggtcg cggactacgt
     6121 ccctttctac ttcgccgcca ggtcgccgat gctctacaca attcattgcg ggaacgtcgc
     6181 ttcgtaccag ggtgggcagg acccgattgt ctacttggtc tcggagatct cgacggtggt
     6241 cgacggcggc tgtactgccg tgttcactga tcggaatgcc tattacccgt acgcaacgtt
     6301 cgacgctgac gctcgtcgtg catacgacga ggttgactgg ccgctgatga gggcgtccat
     6361 gtggaacaac accggcaatg agcccgaccg gatggagcga cgcatggctg agatgctggt
     6421 tcatgacgcg gtgccgttct cgttggtcgt ggagatcgcg gcgcggtcgg aggcgatgct
     6481 cgcgcaagtg aacgacctgc tcgctagtgt cgagcaccgg ccagctctcg ctgtcaggac
     6541 caactggtat ttctagggga tgaccatgat cagagaggct cacggcgact tactcagcgc
     6601 agacgtggat gcgatcgtca acacggtcaa caccgtcggc gtgatgggca aaggtatcgc
     6661 gctccagttc aagaagcggt ttcctgacaa cttcaaggcc tacagcgccg cgtgcaagct
     6721 cggcgacgtg aagctgggtg agatgttcgt cttcgacgct ggacagttag tgacacctcg
     6781 ttgggtgatc aacttcccaa cgaagcagca ctggaagtcc cgatcgcgcc tcaaggacat
     6841 cgacgctggc ctcgacgatc tcgtgcacgt gatcggtgag ttgggtattc agagcatcgc
     6901 cctgccaccg cttggttgcg gattgggtgg gttggattgg ttagaggttc ggccgctcat
     6961 cgaacgcaag ctggccagcg tcgatgcgga catcgtcgtc tatccaccgg agggggcgcc
     7021 gcccgctgcg gcgatcgtcc catcgggcaa tcgcccgacg atgtcggcgg gcaaggctgc
     7081 tttggttcac atcatcgacc gatactcacc ggtcgccatg ggcgcggggc tgatcgagat
     7141 ccagaaactg atgtacttcc tgcaagtcgc cgggcagccc ctcaacctcc actatgtgaa
     7201 gggcatttac ggaccgtacg cggacaacct ccgccacgta ctcaaccggg tcgaaggcca
     7261 ttttctcaca ggattcggtg atggctcagc ctcggtgacc gaagccgagc cgatcgctct
     7321 ccttgatgga gccggagccg aagccgaagt cgtggtggcc gccgacgacc aacttcggcg
     7381 ccggatcgat cgagtgctgg cattggctga cgggtttgag tcgtcgtacg gcatggaact
     7441 acttgcaacc gcccactggg ctgcgtcgag tgagggttgc gacgatcgag cgtgcgtcat
     7501 cgcaaccgtg catggatgga gtaagcgaaa agagcaactc ttcaccgaac gacatgtcgt
     7561 agcggcactc gaccaactcg tcgcgcacgg atggctcggc gaggatttcg ccgcgtaggt
     7621 gcaacgtcgt atgtcgagca gctcac
//
LOCUS       DAOOMA010000037         7621 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_11365_length_7621_cov_3.978192, whole genome shotgun sequence.
ACCESSION   DAOOMA010000037 DAOOMA010000000
VERSION     DAOOMA010000037.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 7621)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7621)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7621
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11365_length_7621_cov_3.978192"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            117..1472
                     /gene="dnaB"
                     /locus_tag="PLP95_01965"
     CDS             117..1472
                     /gene="dnaB"
                     /locus_tag="PLP95_01965"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012223468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HPB44596.1"
                     /translation="MVNTFSPPPGDSRVPPHNLQAEESLLGAMLLKQAAISEAVQIVD
                     TVDFYKPAHGHIFDAISALYAAGEPVDPVTVAAELKRANLLDAIGGPAVLLSLQTRTP
                     AVSNAAHYARLVEENSLLRRLVSTATEIAELGYQPLDDVSKTLDAAESMMYEVAQRRV
                     SDTMVELRDLLDASLDQLEQLYERGDQITGTPSGYTELDELTAGLQPNALIVVGARPA
                     MGKTSFALGAAANAALRSSRPVLFFSLEMGHLELTQRILGSEGRIDATRLRNGQLNEQ
                     DWSKISRAMARLGDAPLWIDDNPNLTIMEIRAKARRLKSKVGDLGLIVIDYLQLMSGR
                     ANAESRQVEISEISRGLKILARELECPVMALSQLSRGLESRADKRPMLADLRESGSIE
                     QDADVVMFVYRDEVYDRDSPDKGTAEIIVAKHRAGPTGVCRLAFLDYCTRFENMAKAA
                     P"
     gene            1893..2603
                     /locus_tag="PLP95_01970"
     CDS             1893..2603
                     /locus_tag="PLP95_01970"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HPB44597.1"
                     /translation="MVSRWSHSGAPRGREYDERFERLARAGHDVHGEASFVMGYAPRR
                     VLDAGCGTGRIAIELLRRGCRVVGVDLDRSMLETAVEKAPEVEWFRADLASFVVPHPT
                     RADHQRLFDAVVCAGNVMIFVQPGTEALVVERMASHLVDGGVLITGFQLHPDGYGVEQ
                     FDADCAAAGLVAEARFSSWSRDPWMIDSGYAVFVHRRPFPEPEPQPEPESGAGAESGE
                     DSETSVSTDVSERDQTEA"
     gene            complement(2588..3142)
                     /locus_tag="PLP95_01975"
     CDS             complement(2588..3142)
                     /locus_tag="PLP95_01975"
                     /inference="COORDINATES: protein motif:HMM:NF013098.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HPB44598.1"
                     /translation="MAFHHVALATNDVAATHHFYTEAMGFTLVKASIGPTPEGGWAKH
                     VFYETGGNGLIAFWDLHGDYGDVKGAMSRDVGLPEWVNHLAFHAPDNDSIDAAMARWL
                     ELGLDVVDIDHDFCRSVYTTDPNGTMVEWCCDTRAFTEAELEHANRMIHSDKPGEFEP
                     APTVRFHQGDRSKRPVWGKDQASV"
     gene            complement(3193..3888)
                     /gene="aat"
                     /locus_tag="PLP95_01980"
     CDS             complement(3193..3888)
                     /gene="aat"
                     /locus_tag="PLP95_01980"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008914 - leucyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HPB44599.1"
                     /translation="MTITQRHLDRLRLGASPWRFPPPEVANPHGFVAAGGDLAPETLV
                     TAYCSGLFPMPLGRRRQIGWWSPDPRGILPLDRLVVSRSLRRSLRRYEVRVDSAFVEV
                     MSNCGDPRRPHGWINKAFIHAYETLHQYGVAHSVECYDDGGRLVGGLYGVSLGGFFAG
                     ESMFHHATDASKVALVALVETLAEVDGALLDVQWVTPHLASLGAIEIPRSDYLDRLTR
                     SLEAPTPTFARLP"
     gene            complement(3940..4791)
                     /locus_tag="PLP95_01985"
     CDS             complement(3940..4791)
                     /locus_tag="PLP95_01985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015798929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxybutyryl-CoA dehydrogenase"
                     /protein_id="HPB44600.1"
                     /translation="MIKRVGICGSGIMGSGIAEVAAKAGFDVVLRSRKQETADATIAK
                     LEKSLAKQVERGRLEAADSAAILARVSGTADVADLADCDLVIESVVEDLETKKALFRE
                     LDAAVKQGAILATNTSTLPIVELAVVTERPELVVGVHFFNPAPAMKLVEIISPITAAA
                     STTDAVTEFAATCGKNPVAVQDRAGFIVNALLFPYLNNAIRMLENGTANREDIDTAMK
                     GGCNFPMGPLELLDLVGLDTSLSILDALYEEFRDPNYAAAPVLRRMVAAGQLGRKSGK
                     GFYDYSS"
     gene            4885..5577
                     /locus_tag="PLP95_01990"
     CDS             4885..5577
                     /locus_tag="PLP95_01990"
                     /inference="COORDINATES: protein motif:HMM:NF015534.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Type 1 glutamine amidotransferase-like
                     domain-containing protein"
                     /protein_id="HPB44601.1"
                     /translation="MTKEVEMSGILALVGGAEWTDGCTFDAKLLQASGTDRVVLIPTA
                     AAYERPQDTIDAATRHFAKYGVTVDVVEVYRRADALAPGAADVLKDAKFVYIAGGSPM
                     HLRSVLKSSPVWDAIVEVWKRGGIIAGTAEGASVLSTFMVDSRGGAYTVGLDFLDGLT
                     VIPRFNTWSEDKLHRTITLAPTGMDIVGIDEATALIWDGAWRVEGSGAVTAFENGKRI
                     EPDQLDPLSTTV"
     gene            complement(5587..6441)
                     /locus_tag="PLP95_01995"
     CDS             complement(5587..6441)
                     /locus_tag="PLP95_01995"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012387.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HPB44602.1"
                     /translation="MGTDKRARQKELSRSRAEAARKEAERAATKRRTLNMVSIVAVAA
                     IIVIAIAVFVGGDDDADVATDGDTTSTTVASDDSTPSTDAAQTGATPTTAVPNIAKAG
                     VGCPEADGSSPHYTGFSEAPEMCIDVDKTYTAEMQTNLGTFTIALDAKRAPNTVNNFV
                     FLARYHYFDGLAFHRIIEDFMFQGGDPTGTGMDGPGYEFDDELPEQGDYQLYSVAMAN
                     SGPNTNGSQFFVITGENGVALPPSYSLFGQVTEGTDVADAIGAVETDSGDLPLEAVVM
                     EKVTITES"
     gene            6527..>7621
                     /locus_tag="PLP95_02000"
     CDS             6527..>7621
                     /locus_tag="PLP95_02000"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012784200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HPB44603.1"
                     /translation="MARLVMKFGGTSVGDPERIRNVADHVARHRRRGDEVVLVVSAMG
                     KETDDLIRVAKEVSGTRPGREMDMLITAGERKAIALVCMALADLGVPAASFTGSQAGF
                     ITDTKHEDAKILEVRADRIREALEAGQVPVVAGAQGVSTDNDVTFLGRGGSDTTAVAL
                     AKALNADAAELYTDVSGVYTTDPRLVETARKMNSISFDELLEMTANGCPKPAMRSVEY
                     AVRHGVKLHVRSAFTWESGTWVVEEDPNMEHATVRAIVHELNEAKVTVTGVADRPGVA
                     ARLFRALADEGVNVDMIVQNASEEGTTDISFTVPHDELATAVSAAEALKDELGIRAVI
                     GDPSVARVSVIGAGMRNNPGIAATMFETLAE"
BASE COUNT         1333 a         2372 c         2534 g         1382 t
ORIGIN      
        1 cccagctcct ccccaagaga aaccacggaa tttcgctgag ttccaccaga aatccccaac
       61 atttccccgc gacatccact ggctcctcac agcccaccaa cgacaggctg gtatcgatgg
      121 tgaacacctt ttccccaccc cccggcgaca gcagagtccc accgcacaac cttcaggcgg
      181 aggagtcgct gctgggcgcg atgctgttga agcaggccgc catctccgag gccgttcaga
      241 tcgtcgacac cgtcgatttc tacaagcccg ctcacgggca catcttcgac gcgatctcgg
      301 cgttgtacgc ggcgggcgaa ccggtcgatc cggtcaccgt tgccgccgaa ctcaaacggg
      361 cgaacctgct cgacgcgatc ggtggcccgg cggtgttgtt gtccttgcag accaggacac
      421 cggcggtgtc gaacgcggcg cactacgccc gcctggtcga agagaattca ctcctgcgcc
      481 ggctcgtgtc gaccgccacc gagatcgccg agctcgggta ccaaccgctc gacgatgtct
      541 ccaagactct cgatgcggcc gagtcgatga tgtacgaggt ggcgcaacgg cgggtgagcg
      601 acacgatggt cgagctgcgc gacctgctcg acgccagcct cgaccaactc gaacagctct
      661 acgagcgtgg cgaccagatc accggcaccc catcgggata caccgaactc gatgagctca
      721 ccgccgggtt gcaacccaac gcgctcatcg tcgtgggtgc ccggccagcg atgggcaaga
      781 cctcgttcgc gctcggtgcg gcggcgaatg cggcgttgcg gtcgagtcgt ccggtgctct
      841 tcttctctct cgagatgggc cacttggagc tcacccagcg tattttggga tcggaggggc
      901 gcatcgatgc gacgcggctt cgcaacggcc agctcaacga acaggactgg tcgaagatct
      961 cgcgtgcgat ggcccgtctc ggcgatgccc cgttgtggat cgacgacaac ccgaacctga
     1021 cgatcatgga gattcgtgcc aaggcccgtc gcctcaagtc gaaagtcggc gacctcggcc
     1081 tgatcgtcat cgactatctc caactgatga gtgggcgggc caatgccgag agccgccagg
     1141 tggagatctc ggagatctcg cgtggtctca agattctggc ccgcgaactc gagtgtccgg
     1201 tgatggcact gtctcagttg tcgcgtggtc tcgagagcag agccgacaag cgcccgatgt
     1261 tggctgacct gcgcgagtcg gggtctatcg aacaggacgc ggacgtggtg atgttcgtgt
     1321 accgcgacga ggtctacgac cgcgattcgc cagacaaagg caccgccgag atcatcgtgg
     1381 ccaaacaccg cgccggcccg accggcgtgt gccggttggc gtttctcgac tactgcaccc
     1441 gtttcgagaa tatggcgaag gccgctccgt aacctcaacg ccgacgcgac gaactgccgg
     1501 cgatcgacga atcggctctg gatggttgag ccgtacacga acaaagcgaa gggccgaagc
     1561 ttgatgcttc ggcccttctg cgctttccag gagttttgct gacccggcgt gacccgtgtc
     1621 tgcatctctg cgatcggcac cacccaatcc gagatgcgga ggggccggtt tcgcttcact
     1681 ccccatcggt ctcagcgacc agtacgtccg atggatctac gttccccgtt gacgagttga
     1741 cctcgtgacc ggtacgaaac caccgagcga cccaaaggtc aaggtattga tcaccgaatg
     1801 agcccgttgg ccttcgataa tgggccgaat gaatcaccga tttcgcgggt ttgtgcgccc
     1861 acgcaccggt tcgaaccggt cacaatggcg aggtggtttc gcgttggtct cacagcggcg
     1921 ccccgcgtgg gcgcgagtac gacgaacggt tcgaacgtct cgcccgcgcg ggccacgacg
     1981 tgcatggtga agccagcttc gtgatgggct acgcgcctcg ccgcgtgctc gatgccggtt
     2041 gcggtaccgg tcgaatcgcg atcgaactcc tgcggcgcgg gtgtcgagtg gtgggggtcg
     2101 acctcgatcg ttccatgctg gaaaccgcgg tcgaaaaggc gccggaggtc gagtggtttc
     2161 gagctgacct cgcttcgttc gtcgtacccc acccgacgcg ggccgaccat cagcgcctgt
     2221 tcgatgccgt ggtgtgcgcg ggcaacgtga tgatcttcgt gcagcccgga accgaagcgc
     2281 tcgtcgtcga gcgcatggca tcgcacctcg tcgacggcgg cgtgctgatc accggtttcc
     2341 agctgcaccc cgacgggtat ggggtcgaac agttcgatgc cgactgtgcg gcagcgggcc
     2401 tcgtggccga ggcaaggttc tcgtcgtggt cgcgcgaccc ctggatgatc gacagcggct
     2461 acgcggtgtt tgtccaccgt cggccattcc ctgaacccga accccaaccc gaacccgaat
     2521 cgggtgcagg tgcggagtcg ggtgaggact ccgaaacgtc ggtgtcgacc gatgtgtccg
     2581 agcgggatca gaccgaggcc tgatccttgc cccacacggg gcgcttggat cggtccccct
     2641 gatgaaaccg cacggtcggc gccggctcga actctccggg tttgtcgctg tggatcatgc
     2701 ggttggcgtg ttcgagttcc gcctcggtga acgcccgcgt gtcgcagcac cattcgacca
     2761 tcgtgccgtt gggatcggtc gtgtacaccg accggcagaa gtcgtgatcg atgtcgacca
     2821 cgtcgaggcc cagttcgagc caccgggcca tggcggcatc aatgctgtcg ttgtcggggg
     2881 cgtgaaatgc gagatggttc acccactccg gtaacccgac gtcgcgtgac atcgcgccct
     2941 tgacgtcgcc gtagtcgcca tggagatccc aaaaggcgat gaggccgttg ccgccggtct
     3001 cgtagaagac gtgcttggcc catccgccct cgggggtcgg gccgatcgac gccttcacca
     3061 acgtgaaacc catggcctcg gtgtagaagt gatgtgttgc ggcgacgtcg ttggtcgcca
     3121 acgccacgtg atgaaatgcc atgtgaacga acccctcgat cgaacttgga aacagcttgc
     3181 catagttggc attcaaggca gccgggcgaa ggtcggcgtc ggggcctcga gtgacctcgt
     3241 caagcggtcc aagtagtcgg atcttgggat ctcgatcgca ccgagggacg ccagatgtgg
     3301 agtcacccac tggacgtcga ggagggcacc gtcgacctcg gcgagggtct cgaccaaggc
     3361 gaccagcgcc actttcgagg cgtcggtggc gtgatggaac atcgattcgc cggcgaagaa
     3421 tccaccgagg ctgactccgt agagtccgcc gacgagtcgt ccaccgtcgt cgtagcactc
     3481 gacgctgtgg gcgacgccgt actgatgcag ggtttcgtac gcgtgaatga atgccttgtt
     3541 gatccacccg tgcggtcgcc gagggtcacc gcaattgctc atcacctcga cgaacgctga
     3601 atcgacccga acttcgtagc gtcgcaagga tcggcgcagt gaacgcgaga ccaccagtcg
     3661 gtcgagtggc agaatcccac gcgggtcggg tgaccaccat ccgatctgtc ggcgacggcc
     3721 gagcggcatc ggaaacagtc ccgagcaata agcggtgacg agggtctccg gcgcgagatc
     3781 gccgccggcg gcgacgaacc cgtgtgggtt cgcaacctcc ggcggcggga atctccaagg
     3841 cgacgcaccc agacggagcc ggtcgagatg tcgttgggtg atcgtcattc gcgtccgttc
     3901 cgaaaggaac cctcgcggcg acgttacccg gacgtcgtgt cagctgctgt agtcgtagaa
     3961 acccttgccg gacttacgtc caagctgtcc ggcggcgacc atgcggcgaa gcacgggggc
     4021 ggcggcgtag ttggggtcgc ggaactcctc gtagagggcg tcgaggatcg acagcgaggt
     4081 gtcgaggccg acgaggtcga gcaactcgag ggggcccatc gggaagttgc agccgccctt
     4141 catcgcggtg tcgatgtcct cgcggttggc ggtgccgttt tccagcatcc ggatggcgtt
     4201 gttgagatac gggaacagca gcgcgttgac gatgaacccg gcgcgatcct gcactgccac
     4261 ggggttcttg ccacaggtag cggcgaactc ggtcaccgcg tcggtggtcg aggcggcggc
     4321 ggtgatcggc gagatgatct cgacgagctt catcgccggt gccgggttga agaagtgcac
     4381 gccgacgacg agttcggggc gttcggtgac gaccgcgagt tcgacgatcg gcagcgtgga
     4441 ggtgttcgtc gcgagaatcg cgccctgctt caccgcagcg tcgagttcgc ggaacaacgc
     4501 cttcttggtc tcgaggtctt cgacgaccga ctcgatcacg aggtcgcaat cggcgaggtc
     4561 ggccacatct gccgtgccgc tgacccgagc gaggatcgct gcgctgtcgg cagcctcgag
     4621 ccggcctcgt tcgacctgtt tcgccaacga cttctcgagc ttggcgatgg tcgcgtcggc
     4681 cgtctcctgc ttacgggagc gcaacaccac atcgaagccc gccttcgcgg cgacctcggc
     4741 gatgcccgag cccatgattc ctgaaccgca gatgcctact cgcttgatca tccgcacaag
     4801 ttacccgtcg gtagcatcgg gtcaccaaaa ccggcccgag gggccgaggc gcgtcgacga
     4861 cgtcggggtc ggtagggtcg gaccatgacg aaggaggtcg agatgagtgg gattcttgcg
     4921 ctcgttggcg gggcggagtg gaccgacggc tgtacgttcg acgcaaagct gctgcaggca
     4981 tcggggaccg accgcgtcgt gttgatcccc accgcggccg cctacgaacg accccaggac
     5041 acgatcgacg ctgcgacgcg gcattttgcg aagtacggcg tcaccgtcga tgtcgtggag
     5101 gtctatcgac gcgccgatgc gttggctccg ggcgcggccg atgtcctcaa agacgcgaag
     5161 ttcgtctaca tcgcgggtgg ctcgccgatg catctgcgat cggtgctcaa gagcagcccg
     5221 gtgtgggatg cgatcgtcga ggtgtggaag cgtggcggga tcatcgccgg cactgcggaa
     5281 ggcgcctcgg tgctgtcgac gttcatggtg gacagccgtg gcggcgccta cacggtcgga
     5341 ctcgatttcc tcgacgggct cacggtgata ccgcggttca atacctggtc ggaggacaag
     5401 ttgcaccgca cgatcaccct cgctccgacg gggatggaca tcgtcggcat cgacgaggcg
     5461 accgcgctca tctgggacgg ggcctggagg gtcgagggtt cgggcgcggt gacggcgttc
     5521 gaaaatggaa agaggatcga gcccgatcag ctcgaccctc tctcgaccac ggtgtgagcg
     5581 ccctgctcag gattcggtga tggtcacctt ctccatcacg accgcctcga gtggcaggtc
     5641 gccggagtcg gtctcgaccg cgccgatggc gtcggcgacg tcggtcccct cggtcacctg
     5701 gccgaacagc gagtagctcg ggggcagcgc aacaccgttt tctccggtga tgacgaagaa
     5761 ctggcttccg ttcgtattcg gcccggagtt cgccatggcg accgaataga gctggtagtc
     5821 gccctgttcg ggcaactcgt cgtcgaactc gtagcccggg ccgtccatgc ccgtgccggt
     5881 cgggtcgccg ccctggaaca tgaagtcctc gatgatgcgg tgaaacgcca ggccgtcgaa
     5941 gtagtggtat cgagcgagga acacgaaatt gttcaccgtg ttcggagcgc gcttggcatc
     6001 gagggcgatc gtgaacgtgc cgaggttcgt ctgcatctcg gcggtgtagg tcttgtcgac
     6061 gtcgatgcac atctcgggtg cctcgctgaa gccggtgtag tgcggcgacg aaccgtcggc
     6121 ttcggggcat ccgacccccg ctttcgcgat gttgggcacc gccgtcgtgg gagtggcccc
     6181 ggtttgagcc gcatcggtgc tcggcgtcga atcgtcggag gcgacggtgg tcgatgtcgt
     6241 gtcgccgtcg gtcgcgacgt cggcatcgtc atcgccaccg acgaacacgg cgatcgcgat
     6301 gacgatgatc gcggccaccg ccacgatcga caccatgttg agtgtccgtc gcttggttgc
     6361 ggctcgttcc gcttccttgc gggccgcctc ggcccgggaa cgggagagct ctttttgacg
     6421 tgcgcgtttg tcggtaccca tagcggccga accttaccaa tccagccccg tctgtctcgg
     6481 ccatcgagct gaaagtgggg tgggatggct cctgtatcgt tgtgccgtgg cccgcttggt
     6541 aatgaagttc gggggcacgt cggtcggcga tccggaacgg atccgaaacg tcgctgatca
     6601 cgttgcccgg caccgtcgac gcggtgacga agtcgtgttg gtcgtctcag cgatgggcaa
     6661 ggaaaccgac gatctcattc gcgtggccaa ggaggtgtct ggcacccggc ccggacgcga
     6721 gatggacatg ctcatcaccg ccggggagcg caaggcgatc gcgctcgtgt gcatggccct
     6781 ggccgatctc ggtgttccgg ccgccagttt caccggtagc caggccgggt tcatcaccga
     6841 caccaaacac gaggacgcga agattctcga ggttcgcgcc gatcggatcc gcgaagcgct
     6901 cgaagccgga caggtaccgg tggtcgccgg ggctcaaggg gtgtcgaccg acaacgatgt
     6961 gacctttctc gggcgaggcg gatcggatac gacggcggtg gccctcgcca aggcgctgaa
     7021 tgcggatgcc gccgagctct acaccgatgt gtcgggcgtc tacacgaccg accctcgtct
     7081 ggtggagacc gcccgcaaga tgaactccat cagcttcgat gagttgctgg agatgaccgc
     7141 gaacggctgc cccaagccgg cgatgcggtc ggtcgaatac gccgtgcgcc acggcgtgaa
     7201 actgcacgtt cgctcggcct tcacgtggga gtccggcacc tgggtggtcg aggaggaccc
     7261 gaacatggag cacgcaaccg tccgagcgat cgttcacgaa ctcaacgagg ccaaggtcac
     7321 cgtcaccggt gtggccgacc ggcccggggt tgcggcacgg ctgtttcgtg cgctggccga
     7381 cgagggggtg aatgtcgaca tgatcgtgca gaacgcgtcc gaggagggca ccaccgacat
     7441 ttccttcacg gtgccccacg acgaactcgc caccgccgtg tcggccgccg aggcgctgaa
     7501 ggacgaactc ggaattcgtg ccgtcatcgg cgatccctcg gtggcgcggg tgagcgtcat
     7561 cggcgccggg atgcgcaaca accccggtat tgcggcgacg atgttcgaaa ctctggcgga
     7621 g
//
LOCUS       DAOOMA010000038         7585 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_11428_length_7585_cov_3.550465, whole genome shotgun sequence.
ACCESSION   DAOOMA010000038 DAOOMA010000000
VERSION     DAOOMA010000038.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 7585)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7585)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7585
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11428_length_7585_cov_3.550465"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            47..184
                     /gene="mftA"
                     /locus_tag="PLP95_02005"
     CDS             47..184
                     /gene="mftA"
                     /locus_tag="PLP95_02005"
                     /inference="COORDINATES: protein motif:HMM:TIGR03969.1"
                     /note="Mycofactocin is a small molecule electron carrier
                     derived from the final two amino acids, Val-Tyr, of MftA,
                     the mycofactocin precursor. It plays a role in redox
                     homeostasis and the metabolism of alcohols and aldehydes
                     in Actinobacteria, including Mycobacterium tuberculosis;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0140604 - mycofactocin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mycofactocin precursor MftA"
                     /protein_id="HPB44604.1"
                     /translation="MSSEATVVEELEAVANDVAPSADASLVEEELLVEEISIDGMCGV
                     Y"
     gene            224..505
                     /gene="mftB"
                     /locus_tag="PLP95_02010"
     CDS             224..505
                     /gene="mftB"
                     /locus_tag="PLP95_02010"
                     /inference="COORDINATES: protein motif:HMM:TIGR03967.1"
                     /note="MftB, a small protein, is a peptide chaperone that
                     assists the radical SAM enzyme MftC in performing two
                     modifications to the C-terminal Val-Tyr dipeptide of the
                     mycofactocin precursor peptide, MftA. MftB's role is
                     analogous to the role of PqqD in the biosynthesis of PQQ,
                     a cofactor that derives entirely from a Tyr and a Glu in
                     the precursor PqqA; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0140604 - mycofactocin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mycofactocin biosynthesis chaperone MftB"
                     /protein_id="HPB44605.1"
                     /translation="MTNAVRALLDRPYELDPMVSLRPEPFGALAYHFGNRRLSFLRNH
                     DMVRVVESLGAHDSVADALEAAGVERRRWPSFEKALATLESSEMIRVRA"
     gene            498..1787
                     /gene="mftC"
                     /locus_tag="PLP95_02015"
     CDS             498..1787
                     /gene="mftC"
                     /locus_tag="PLP95_02015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012226361.1"
                     /note="MftC is a radical SAM/SPASM enzyme that catalyzes
                     the first two steps in biosynthesis of the electron
                     carrier mycofactocin from the terminal Val-Tyr dipeptide
                     of the precursor peptide MftA; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0031179 - peptide modification [Evidence
                     IEA];
                     GO_process: GO:0140604 - mycofactocin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mycofactocin radical SAM maturase"
                     /protein_id="HPB44606.1"
                     /translation="MHEPLELNVQSGAAPAVLAPNGRTTIPVITTQPPAPKRSTLTDD
                     MKRGLDAPICLTWELTYACNLACEHCLSSSGRRDPRELSTEECFAVIDELERLQVFYV
                     NLGGGEPMLRPDFFEIIEYAVSHHVGVKFSTNGTYIDAETATRLASMDYLDIQISIDG
                     ATEDINDPLRGDGSFAKAIAAMEHLYAAQFGPFKISVVVTRNNVDQLDDFAAIAARYG
                     AQLRVTRLRPSGRGADSWDELHPTMDQQRQLYHWLKDRPEVLTGDSFFHLSAFGEALP
                     GLNLCGAGRVVCLIDPVGDVYACPFVIHDEFLAGNVRDTGGFTAVWRNSELFTSLRQP
                     SSPGACGSCGSYDACQGGCMASKFFVGLELDDPDPECVKGHAEELLAARVPGTAPVPS
                     VDHSRPGIERTFGRTGVSIGAKPQPVEARVSEPATFT"
     gene            1784..3835
                     /locus_tag="PLP95_02020"
     CDS             1784..3835
                     /locus_tag="PLP95_02020"
                     /inference="COORDINATES: protein motif:HMM:TIGR03996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mycofactocin system FadH/OYE family
                     oxidoreductase 1"
                     /protein_id="HPB44607.1"
                     /translation="MSLTDPLRIGPFEAPSRVMFGPHETNLAHGRALSDRHVAYYERR
                     AAGGAGVIVVEEASVHGSDWPYERAPLAADAGPGWSAIAAACHHHGAVVLAALGHSGG
                     QGSSAYSQMPLLAPSRVPEVNTREVPKWMEVDDIDVVVSSFAEAARSAVEAGCDGVEI
                     NAGQYSLIRQFLSALTNQRGDEWSAGTRFAREVLTAVRDAVGADHIVALRLSADELAP
                     WAGIVPEVGAQIAAELAEWVDLITVVRGAIFSTAATRPDGHTEPGFNLELASQVRDAV
                     RERHGERVAVFAQGSIVDVGQARWALDEARCDGVEMTRAQIADADLVAKVRADRAAHI
                     RPCILCNQTCNVRDARNPIVTCVVDPRSGYEWHDAEPAVAGVSNDQRPKRSVVVVGGG
                     PAGLEAARVAALAGRSVTLHERRGRLGGMTTVAAAGSGRSRLAAIAEWLAVEVERLGV
                     VVRLGSEVAAGDLDPDADVVIATGSVDAPASYKVSRSAVVVTAAEFLERGEAVLPDGP
                     VFVWDPIGGPVAVSIAERLSAPGEGPSRSVVLATQDNIVGNELSRSGDLAPANARLQQ
                     AGVVLERRTLLRSVRKGGVEVEDRFSGETRTIKAAVVIDCGHRLPDEDLYRAVSSDDD
                     RTAAASRRRVVRAGDSVAPRTILEAIREGRAAIAALDGDVAVSDGVAMGPKRLGSEAG
                     H"
     gene            3835..5817
                     /locus_tag="PLP95_02025"
     CDS             3835..5817
                     /locus_tag="PLP95_02025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020378755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mycofactocin system FadH/OYE family
                     oxidoreductase 2"
                     /protein_id="HPB44608.1"
                     /translation="MAEQMLWSPLRLGPVTLKNRVVFSAHLTNYATQDGKPSDQHVAY
                     YAERAKGGAGLIITEEHSTHPTDWPYEKLIHGFNPAVIDGYRRITEAVHRHGTPIFAQ
                     LNHNGGQASSMFSRLPVWAPSAVPDPLFREVPKAVSLAEIAEIVESYALVARHCREGG
                     FDGIELQCSHSSIVRGFLSPATNKRTDGYGGSIPNRARILVEIVEAVRQAIGPSLALG
                     VRICGDELIDGGTTIDDALEVAKIVEATGNVDYINTSIGVATASLFMIEASMHVPPNY
                     AMFIPSAIRKVVDLPVVGVGRFKDPLQAERALQEGHCDLVGVVRGQIADADFARKARA
                     GHTDDTRLCLSCNQECVGRMGLNRWLGCIENPRTGRESQGVAEVRLTTAPKQVLVVGA
                     GPAGLQAAIAAARNGHRVTVVEAHAEPGGQVRLAASVPNRAEFGDLVRNQVSECRRLG
                     VSIEYGISVDAAEVRRRGPDSVVVATGARAARPWWAPGDAEWIVDVRDVLDGSTHPDG
                     QVIVLDELGFHHATSVAELLADRGARVEVITNGMVVGQDLGITLDMENWWIRANAKGI
                     VQSTELVPMGVSVDEGGTKTVEFQHHPTGQMRSRTPDWVVLAVPADPVEELYLELSGD
                     ESLRAAGVGVERVGDCVAPRRAHAAIIEGERVGAGL"
     gene            complement(5852..6349)
                     /locus_tag="PLP95_02030"
     CDS             complement(5852..6349)
                     /locus_tag="PLP95_02030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB44609.1"
                     /translation="MTPTRRLALAATLASTLVLGACGDTGSEDAAVTTTTASSGEEAT
                     TNSTSSDGSDHGVMSSESDALQCEMHMPGDLLTAEQAHVIMDTEHVCLGYVTVTTETP
                     VTFNNTDTEPRTVAILDDAGTELESFEIPAGGAVVRTFDTVGVFRFSYSGIEIFTGTV
                     EVQAP"
     gene            complement(6349..>7585)
                     /locus_tag="PLP95_02035"
     CDS             complement(6349..>7585)
                     /locus_tag="PLP95_02035"
                     /inference="COORDINATES: protein motif:HMM:NF019352.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="multicopper oxidase domain-containing protein"
                     /protein_id="HPB44610.1"
                     /translation="SEFAIKGDLNATPGKITINITNDGSVAHDFQIADLGILTDKIAP
                     GVSTTVVIEDAAAGSYEIICTVAGHKESGMTATLTVGESGGSASGGSSSHDHTSLTPE
                     EAQARDDNMMAGMMSFGTDEAATEGRGNTPAEFTIAADGAKEFELTAAITKWEVETGK
                     FVDAWTYNGQVPGPWFKVDVGDRVRIKVTNNLPLGTDIHWHGIHLPNEMDGVAPYTQD
                     PIAPNGGTFTYEFTADTRNNGMYHAHMHGEEAVPNGMLGVFQVGDVELPRGRTVSGIN
                     VPNDVNIVQEIPMVLNDAGAIGYSLNGKSFPSTEPVVVNKDDWFLVTYYNEGLQVHPM
                     HMHGFPQLVVARDGITLDQPFWVDTITVGPGERYSILVKATDLGTWVWHCHILNHVET
                     PERMFGMVTAVVVQDPAAA"
BASE COUNT         1237 a         2339 c         2627 g         1382 t
ORIGIN      
        1 cgctgtagct ttggttgacg tttcacgccg cccaagggga ggattcatga gctccgaagc
       61 cactgtcgtc gaagaactcg aagccgtcgc caacgacgtt gcgcccagcg ccgacgcatc
      121 gctggtcgaa gaggaactgc tcgtcgagga gatctccatc gacggcatgt gcggcgtcta
      181 ctgatccgca gctggtcagt agaagatcga aacggcccac ccgttgacga atgcggttcg
      241 agcgctgctc gatcggccct acgaacttga tccgatggtt tcgctgcggc ccgaaccgtt
      301 cggcgcactc gcgtaccatt tcggcaaccg ccgcctgagc ttccttcgta accatgacat
      361 ggtgcgcgtc gtcgaatccc tcggcgcaca cgacagtgtc gccgatgcgc tggaggcggc
      421 cggagttgag cggcggcgat ggccgtcgtt cgaaaaagcg ctcgccaccc tcgagtcatc
      481 ggagatgatc cgtgtccgtg catgaacctc tcgaactgaa cgtccagagc ggcgctgcgc
      541 cggccgtact cgcgccgaat ggacgtacaa cgattccggt gatcacgacg cagccgccgg
      601 cgccgaagcg gtcgacgctg accgacgaca tgaaacgggg cctcgatgct ccgatctgtc
      661 tgacgtggga gctcacctac gcctgcaacc tcgcgtgcga gcactgcctg agcagctccg
      721 ggcgccgcga tcctcgcgag ttgagcaccg aggagtgctt cgcggtcatc gacgaactcg
      781 aacgactcca ggttttctac gtgaacctcg gcggcgggga accgatgctg cggcccgact
      841 tcttcgagat catcgaatac gcggtgagtc atcacgtcgg ggtgaagttc tcgaccaacg
      901 gcacctacat cgatgctgag accgcgacgc ggctggcgtc gatggactat ctcgacatcc
      961 agatttccat cgacggcgcg accgaggaca tcaacgatcc gctgcgcggc gacgggtcct
     1021 tcgccaaggc catcgcagcg atggaacacc tgtacgccgc ccagttcggt ccgttcaaga
     1081 tctcggtggt ggtgacgcgt aacaacgtcg atcagctcga cgatttcgcc gcgatcgcgg
     1141 cgcgttacgg ggcacagctc cgggtgacgc gcctgcgtcc ctcaggacgc ggcgccgaca
     1201 gctgggatga gctgcacccg acgatggatc agcagcgtca gctctatcac tggttgaaag
     1261 accggcctga ggtgctgacc ggcgactcgt tcttccacct ttccgccttc ggtgaagcgc
     1321 tgccgggtct caacctgtgt ggtgcgggcc gtgtggtctg tcttatcgac ccggtcggcg
     1381 acgtgtacgc atgcccgttc gtcattcacg acgagttcct ggcgggcaac gtgcgtgaca
     1441 ccggcgggtt cacggcggtg tggcgcaact cggagttgtt cacctcgttg cgtcagccga
     1501 gttcgcccgg ggcctgtggt tcctgtggga gctacgacgc atgccagggt ggttgcatgg
     1561 cctcgaagtt cttcgtcgga ctcgagctcg acgaccccga cccggaatgc gtgaagggcc
     1621 atgccgagga gctgttggcg gcccgggttc cgggaacggc gccggttcct tccgtcgacc
     1681 acagtcggcc aggaatcgag cgaaccttcg ggcgcaccgg tgtctccatc ggcgccaagc
     1741 cgcaacctgt cgaggctcga gtttccgaac cggccacgtt cacgtgagtc tcaccgatcc
     1801 actccgaatc ggcccattcg aagcgccgag ccgtgtcatg ttcggccctc acgagaccaa
     1861 cctggcacac gggcgtgcgc tgtcggaccg tcacgttgcc tactacgaac gccgggccgc
     1921 gggaggcgca ggcgtcatcg tggtcgagga agcctcggtg cacggaagcg actggcccta
     1981 cgaacgagcg ccgctggcgg cggacgctgg tccgggctgg tcggctatcg ccgcagcctg
     2041 ccatcaccac ggtgcggtgg tgctcgcggc gctcgggcat tcgggcgggc aagggtcctc
     2101 ggcctactcc cagatgccgc tgcttgcgcc ttcgagggtt cccgaggtca acacccgcga
     2161 ggtgcccaag tggatggagg tcgacgacat cgatgtggtc gtgtcgtcgt tcgccgaggc
     2221 ggcgaggagc gcggtcgagg ccggctgcga cggggtggag atcaacgccg ggcagtactc
     2281 gctcatacgt cagtttctct cggcgctcac caaccagcgc ggtgacgagt ggtcggcggg
     2341 aacgaggttc gcacgtgagg ttctgactgc ggttcgagac gcggtcggcg ccgaccacat
     2401 cgtggcgttg cggctgagcg ccgatgagct cgccccgtgg gccggcatcg tcccggaggt
     2461 cggggcgcag atcgccgccg aactcgccga atgggtcgac ctgatcaccg tcgtgcgcgg
     2521 tgcgatcttt tcgaccgcgg ccacacgtcc ggacggtcac accgagcccg ggttcaacct
     2581 cgagctggcg tcgcaggtgc gcgacgcggt gcgcgaacga cacggcgaac gcgtcgcggt
     2641 gttcgctcag ggctcgatcg tggatgtcgg ccaagcgcgt tgggccctcg acgaggcacg
     2701 ttgcgatggc gttgaaatga cccgcgccca gatagcggac gccgacctcg tcgccaaggt
     2761 ccgggccgac cgcgctgcgc acattcgtcc gtgcatcctg tgcaatcaaa cgtgcaacgt
     2821 gcgcgatgcc aggaacccga tcgtgacctg tgtggtcgac ccgcgaagcg gctacgaatg
     2881 gcatgacgcg gaaccggccg tcgccggggt ttcgaacgat cagcgcccga agcgctccgt
     2941 tgtggtggtc ggcggcggcc ccgcagggct ggaggccgcg agggtcgcgg ccctcgcagg
     3001 ccgctcggtg acgttgcacg aacgacgcgg acgcctgggc ggcatgacga cggttgcggc
     3061 agcgggctct ggacgttcgc ggctcgccgc gatcgccgag tggctcgcag tcgaagtcga
     3121 acggcttgga gtcgtcgttc gattgggctc agaggtcgcg gcaggcgacc tcgacccgga
     3181 cgctgacgtg gtgatcgcca ccggttcggt ggacgcgccg gcgtcctaca aggtgtcgcg
     3241 ttcggcggtg gtggttaccg cggctgagtt tctcgagcga ggcgaggcgg tcctgcccga
     3301 tggtcctgtc ttcgtctggg atccgatcgg gggcccggtt gccgtttcga ttgcggagcg
     3361 attgtcggca cccggcgagg gaccgtcacg atcggtcgtg ttggccacac aggacaacat
     3421 cgtcggaaat gagctgagtc gttcgggcga cctggcgccg gcaaacgctc ggctgcagca
     3481 ggccggtgtc gttctggagc gtcgaacact gctgcgatcc gtccgaaagg gtggcgtcga
     3541 ggtcgaggat cgtttcagcg gcgagacgcg cacgatcaag gccgccgtcg tcatcgactg
     3601 tggccaccgg ctgcctgacg aggacctgta tcgagccgtg tcgtcagacg atgaccggac
     3661 cgccgcggcg tcgcgacgtc gggtggtgcg ggctggcgac tcggttgccc cgcgcacgat
     3721 tctcgaggcg attcgtgagg gtcgcgccgc gatcgcagcg ctcgacggag atgtcgccgt
     3781 gagcgatggc gtggcgatgg ggccgaagcg gctgggttcg gaggcagggc actgatggct
     3841 gagcagatgt tgtggtcgcc gttgcggctc gggccggtga ccttgaagaa ccgggtggtg
     3901 ttctcggccc acctgacgaa ctatgcgacc caggacggca agccctccga ccaacacgtc
     3961 gcctattacg ccgagcgagc gaagggtgga gccggcctca tcatcaccga agagcactcg
     4021 acccatccga ccgactggcc ctatgagaag ctgatccacg ggttcaaccc ggcggtgatc
     4081 gatggctacc gccggatcac cgaggcggtc caccgtcacg gaacgccgat cttcgcacaa
     4141 ctcaaccaca acggcggtca ggcgtcgtcg atgttcagcc gcttgccggt gtgggcgccc
     4201 tcggcggtgc ccgacccgct gtttcgcgag gtccccaagg cggtcagcct cgcggagatc
     4261 gcggagatcg tcgagagcta cgcactcgtg gcacgccatt gccgcgaggg cggcttcgac
     4321 gggatcgagt tgcagtgctc tcactcttcg atcgtgcgcg ggttcctctc gccggcgacg
     4381 aacaagcgaa ccgacggata cggcggctcg atccccaatc gggcgcgaat tctggtggag
     4441 atcgtggagg cagtgcgcca ggcgatcggc ccctcgctcg cgttgggggt gcgcatctgc
     4501 ggcgatgagt tgatcgacgg gggtaccacg atcgacgacg cgctcgaggt cgccaagatc
     4561 gttgaagcca ccggaaacgt cgactacatc aacacctcga tcggcgtggc gaccgcctcg
     4621 ctgttcatga tcgaggcgtc gatgcacgtc ccgccgaact acgcgatgtt cattccgtcg
     4681 gccatacgca aggtcgtcga tcttccagtg gtcggggtcg gtcgtttcaa agacccgctt
     4741 caggcggagc gagcgctgca ggaaggtcat tgcgatctgg tgggcgtcgt gcgcggtcag
     4801 atcgctgatg ccgatttcgc ccgcaaggcc cgtgccggtc acaccgacga cacacgattg
     4861 tgtctgagct gcaaccagga atgcgtcggt cgcatgggcc tgaaccgctg gctgggttgc
     4921 atcgaaaacc cgcgcacggg ccgcgaatcc caaggggtcg ccgaggtgcg gctcaccacg
     4981 gcaccgaagc aggtcctggt ggtgggtgct gggccggcgg ggctgcaggc ggcgatcgct
     5041 gcggcccgca acggccaccg ggtcaccgtg gtcgaggcgc atgccgagcc cggcggtcag
     5101 gtgcgccttg ccgcgtcggt gcccaaccgc gccgagttcg gcgacctcgt tcgtaaccag
     5161 gtcagcgagt gtcgacgcct cggcgtgtcg atcgagtacg gcatcagcgt cgacgcggcg
     5221 gaggtccggc gccgtggccc cgactccgtg gtcgtcgcca ccggagcccg cgccgcgagg
     5281 ccgtggtggg cgccgggcga tgccgaatgg atcgtcgacg ttcgcgacgt cctggatggg
     5341 tcgacccatc cggacggcca ggtgatcgtg ctcgacgagc ttgggtttca ccacgcgacc
     5401 tcggtggccg aactcctcgc agaccgtggc gcgagggtgg aggtcatcac caatggaatg
     5461 gtcgttggcc aagacctcgg catcaccctc gacatggaga actggtggat tcgtgccaat
     5521 gccaagggca tcgtgcagag caccgaactg gttccgatgg gtgtgagcgt cgatgagggc
     5581 ggcaccaaga ccgtcgagtt ccagcatcac cccacgggac agatgcgctc acgcaccccc
     5641 gattgggtgg tgctggcagt gccggccgac ccggtcgagg agctgtacct cgaactgagc
     5701 ggtgacgagt ccttgcgtgc ggcgggagtc ggtgtcgaac gggtcggaga ctgtgtcgcc
     5761 ccacgtcgcg cacacgcagc gatcatcgag ggtgaacgcg tcggagccgg cctgtgagca
     5821 ttggcgcagc ggacgccgcc gacgaacagc tctagggagc ttgaacctct acggtgccgg
     5881 tgaagatctc gattccgctg tagctgaatc gaaacacccc gacggtgtcg aaggtgcgca
     5941 ccaccgctcc acctgccggg atctcgaagg attcgagttc ggtaccggcg tcgtcgagta
     6001 tcgcgacggt gcggggctcg gtgtcggtgt tgttgaacgt caccggggtt tcggtggtga
     6061 cggtgacgta accgagacaa acgtgttcgg tgtccatgat cacgtgtgcc tgttcggcgg
     6121 tgaggagatc gcctggcatg tgcatctcgc actgaagcgc atcgctctca ctcgacatca
     6181 ccccgtggtc ggagccatcg ctcgaggtgc tgttcgtggt ggcttcctca cctgaagaag
     6241 ctgtggtcgt cgtgaccgca gcgtcctcgg agcccgtgtc gccacaggct ccgaggacga
     6301 gggttgacgc cagtgtcgcc gcgagggcga ggcgccgcgt cggggtcatc aggccgctgc
     6361 aggatcctgg acgacgacag cggtcaccat accgaacatg cgctctggtg tttccacgtg
     6421 gttgaggatg tggcaatgcc agacccatgt tccgaggtcg gttgccttca ccaggatgga
     6481 atagcgctca ccgggaccga ccgtgatcgt atcgacccag aagggttgat cgagggtgat
     6541 gccgtctcgt gccaccacga gttgcgggaa cccgtgcatg tgcatcgggt gaacctgcag
     6601 accttcgttg tagtaggtca cgaggaacca gtcgtccttg ttcaccacga ccggctcggt
     6661 ggaggggaag ctcttgccgt tgaggctgta gccgatcgct ccggcatcgt tgaggaccat
     6721 cggaatttcc tggacgatgt tcacgtcgtt gggaacgttg atacccgaga cggtcctgcc
     6781 gcgtgggagt tcgacgtcgc cgacctggaa cacgccgagc atgccgttgg ggacggcctc
     6841 ctcgccgtgc atgtgagcgt ggtacatgcc gttgttgcgg gtgtcggcgg tgaactcgta
     6901 ggtgaatgtt ccgccgttgg gagcgatcgg gtcctgggtg tagggggcca caccgtccat
     6961 ctcgttgggg agatgaatgc cgtgccagtg gatgtcggtg ccgagtggaa ggttgttggt
     7021 caccttgatg cgaacgcgat cgccgacgtc gaccttgaac cacgggccgg gaacctgacc
     7081 gttgtaggtc caggcatcga cgaacttgcc ggtttcgacc tcccacttgg tgatggcggc
     7141 ggtgagttca aattccttgg ctccgtcggc ggcgatggtg aactccgccg gggtgttgcc
     7201 acgaccttcg gttgcagcct cgtcggtgcc gaaggacatc atgccggcca tcatgttgtc
     7261 gtcgcgtgcc tgtgcttcct cgggggtcag cgaggtgtgg tcgtggctgc tcgaccctcc
     7321 agaggcggag ccacccgact cgccgacggt caacgttgcg gtcattccgg attccttgtg
     7381 gccggcgacc gtgcagatga tctcatagga gccagcggcg gcatcttcga tcacgacggt
     7441 ggtcgacaca ccgggagcga tcttgtcggt gaggatcccg aggtcggcga tttggaagtc
     7501 gtgggcgacc gaaccgtcgt tggtgatgtt gatcgtgatc ttgcccgggg tggcgttcaa
     7561 gtcgcctttg atcgcgaact cgctg
//
LOCUS       DAOOMA010000039         7516 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_11578_length_7516_cov_4.835009, whole genome shotgun sequence.
ACCESSION   DAOOMA010000039 DAOOMA010000000
VERSION     DAOOMA010000039.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 7516)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7516)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7516
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11578_length_7516_cov_4.835009"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            174..1988
                     /locus_tag="PLP95_02040"
     CDS             174..1988
                     /locus_tag="PLP95_02040"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HPB44611.1"
                     /translation="MILVDLVKVGASRPGKPLFEDLSLTINTGDRLGIVGINGCGKST
                     LMRAMAGIESPESGAVRFGNGVRIAALDQEPRFEGGTVHEAAGATWESDAVLDRLGMG
                     AYLDVSVDRLSGGQSKRVALARALVAESDLLILDEPTNHLDIDAIAWLEERLEAYNGG
                     LVMVTHDRHVLDRVTNRVLELDRGRAYLHDGGYDTYLENKADRAAQAARAEEVRKNLA
                     RTELAWLRRGAPARTSKPKHRIATATAIVEGRAQAEARTGDLPLHFGTPRLGNEVVEL
                     HDVGHRWDEGSPWLFEHVDLLLDPRERLGLVGPNGTGKSTLLEIVAGRIDPTAGRVKV
                     GQTAKVGYFDQRGRSLDPEARVREVVAGPNRTPDYTDAALMEAFWFDGDAQYAPVRLL
                     SGGERRRLQLLVTLAEKPNVLLLDEPTNDLDLDTLRALEDFLDEWPGALIVVSHDRAF
                     LERTVADVIVMDGTGFAGRRPGGYAAYEEERRAAPRRRGSVAAARAASTTGGGGKARA
                     SSETKASRSSGSAEGGEKLRSPSTLRHRFRAVERETETLQAERDRINAELVEHASDHV
                     RLAGLAAELQAVVEALEPIEEEWLELAAEAEERGLDLS"
     gene            complement(2102..3349)
                     /locus_tag="PLP95_02045"
     CDS             complement(2102..3349)
                     /locus_tag="PLP95_02045"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl sulfatase dimerization domain-containing
                     protein"
                     /protein_id="HPB44612.1"
                     /translation="MTSLFETSEKILDGRLSIAAHHPVSIASETTLEPISDRVAFMET
                     FANVTAIRDGDELGIVDAGSPLHAPGIVDATRRWTPNPLRTAVFTHGHVDHIYAVKLF
                     EAEGNGSAKVIAHEAVADRFRRYDLTNGYNGVINQRQFQLDAPFFPGDYRYPDETYRS
                     TTSTSVGSVDLQLRHDRGETDDSTWVWIPEEKVLCTGDLFIWASPNCGNPQKVQRFCD
                     EWAVALRAMAALEPEYLLPGHGLPIAGAARVRTVLEDTARYLESLFDQTIEMMNAGAR
                     LTDILATVRPPTDLEDRPWLQPVYDEPEFIVRNIWRLYGGWYDGNPANLKPAPDAAVA
                     SEIAALAGGADRLVERALELAEGDEFRLAGHLIETASLAAPDSVEVRRARAAIFGRRA
                     EVERSTMAKGVFSWAAKESNPDT"
     gene            3581..4471
                     /locus_tag="PLP95_02050"
     CDS             3581..4471
                     /locus_tag="PLP95_02050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB44613.1"
                     /translation="MKQTHAAVAGIVAVTASLAFAGVAGAQDGDGAQTVESTTVESTT
                     VESSTPDTTAPEQSTSSTTPESTVPEPASPIEVPADEAPPADPFEVLRGVDLPGVAVT
                     SGTLSFQVSFSDQPASSCGGNLAMSGGGMNVAYDEAGGVGGAYGLVETGSGTFGTLFV
                     GFGPIPVGVGIISVSDENCEFDAVGIGTYSASASTAELAGVGIGIHPGAYETGDYVSE
                     FTITAGVGATGGEATLDVDRLGAFLLRDRPGLNLALPGSPSVPATPPMPETPAETPVV
                     PGEPQTPTSAEPPATAPTAQ"
     gene            4579..5772
                     /locus_tag="PLP95_02055"
     CDS             4579..5772
                     /locus_tag="PLP95_02055"
                     /inference="COORDINATES: protein motif:HMM:NF012656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HPB44614.1"
                     /translation="MDFDDSPEQAEFRREARAWLDANAEVRDGESSMWRALRPQNDAD
                     DRWALQRARQWQATKYGGGYTGLHWPVEVGGRGLSPHIAGVFKQEESRYDVPANAFQV
                     GVDMVGPTLIRHASKDLCRRHLDTTLRGDDFWCQLFSEPGAGSDLAGLTTRAFRDGDR
                     FIVNGQKVWTTTAHWATHGLLLARTNPEAPKHKGITAFVVDMSTPGIDIRPLRQADGA
                     EHFNEVFLTDVEIPADNVVGAVDDGWAVAHTMLSSERSAIAGGGMVQFDEILMLARDR
                     DRGEDPLVRQDLARLYSYFEISRFLGYRVQTALSHGRTPGPESSVMKLHISRQYEFGG
                     DVMMSILGAAAMSWGDSAPFGGFFQGVFMAQWAPRIGGGTDQIQRNIVGERVLGLAGE
                     PRRDR"
     gene            complement(5813..7075)
                     /locus_tag="PLP95_02060"
     CDS             complement(5813..7075)
                     /locus_tag="PLP95_02060"
                     /inference="COORDINATES: protein motif:HMM:NF013895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HPB44615.1"
                     /translation="MARSDGSLGALRPDRLARRLVDLLGPTGGAARQSVSALALNSTT
                     SFAAGAMLVGFQDTWHNLSPLLIMVPAAIGLRGNVFSTLGNRLSTAIHTGTFRVSFKR
                     DSVLGQNLLVSFSLTLVMSVVLAAIAKVLGLALGTSQDISMLELTMVSGIGGLLGSIV
                     VAAATVLLTIGAVRYEWDLDYLVAPTVSTLGDGITIPALWLAAQLVGGHNVDSFLGLL
                     IIVSAVVIGIWTWRSALDLTRQVFRESLPVLFFALILSAFAGVVLQRQENLLAIVPAI
                     GVLQPAFVSSAGALGGILCGRIATNLHIGLVSPTLMPGTQARLDASSIFGLAVPLLVF
                     NVFGAWLATNLSGHAAAPGFWWLLLTSCLASVATLAFVATLAYYSTVGAWRFNVDPDT
                     YGTPLVTASVDFVGTMALVVSAVALGLL"
     gene            complement(7132..>7516)
                     /locus_tag="PLP95_02065"
     CDS             complement(7132..>7516)
                     /locus_tag="PLP95_02065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB44616.1"
                     /translation="GVFGTADAGWQQRYPAAVEVIQRPPWNVVGFIESASSDAATVSV
                     RLQTPSATQAADSVELSGATDGAGEDGTSCAAAEPGDAVACRLPPAAADDTTVVEFRA
                     PRASPGRSLFLSASNAERATVLTWR"
BASE COUNT         1333 a         2374 c         2621 g         1188 t
ORIGIN      
        1 cgtgcgcgag cacgacgaac ctaccggcga gcgtccagga cgcctgttgc gcggcggcca
       61 accggcgccg agccgcatct gaaccgtcgt cgacgacggc gcaacggttc gggcagcgcc
      121 ccaaggctga acggttctcg acgggaagcc cgcgccggta gcgtggactc acgatgatcc
      181 tggtcgacct cgtcaaggtg ggcgcctctc gccccggaaa accgctgttc gaggacctgt
      241 ccctcacgat caacaccggc gaccgcctcg gcatcgtcgg catcaacggg tgcggaaagt
      301 ccacgctcat gcgggcgatg gccggcatcg aatcgccgga gtcgggcgca gttcgctttg
      361 gcaacggtgt gcgaatagcg gctctggatc aagagccgcg gttcgagggc ggaacggtgc
      421 acgaagccgc cggtgcgaca tgggaatccg acgcggtgct cgatcggctc ggcatggggg
      481 cgtacctcga cgtgtcggtc gatcggctct ccggcgggca atcgaaacgc gtggccctcg
      541 cccgtgcgct ggtggccgag tcggacctgc tcatcctcga cgagccgacc aaccatctcg
      601 acatcgacgc catcgcctgg ctcgaggagc gtctcgaggc ctacaacggc ggtctcgtga
      661 tggtcaccca cgaccgtcac gtgctcgatc gtgtcaccaa ccgggtgctc gaactcgacc
      721 ggggccgggc ctatcttcac gacggcggct acgacacgta tctggagaac aaggcagatc
      781 gcgccgctca ggccgcacga gccgaggaag tgcgtaagaa cctcgcgcgc accgagttgg
      841 cgtggctgcg gcgtggggcc cctgcgcgta cttccaagcc gaagcaccgt atcgccacgg
      901 caacggccat cgttgagggc agggcccagg ccgaggcgcg aaccggagac ctgccgctgc
      961 acttcggcac gccgaggctg ggcaatgagg tcgtggaact gcatgacgtc gggcatcggt
     1021 gggatgaggg ctcgccgtgg ctattcgaac acgtcgatct gttgttggat ccgcgtgagc
     1081 ggctcggcct cgtcggcccg aacggcacgg gcaagtcgac cttgctcgaa atcgtcgcgg
     1141 gccgaatcga tccgactgcg ggacgggtga aggtcggcca gaccgcgaag gtcggctatt
     1201 tcgaccaacg aggacgttcc ctcgaccccg aggctcgggt gcgtgaggtc gtcgccggtc
     1261 ccaaccgaac gccggactac accgacgccg cgttgatgga ggcgttctgg ttcgacgggg
     1321 atgcccaata cgccccggtt cgcttgttga gcggcggcga gcgtcgccgc ctgcagttgc
     1381 tcgtcaccct cgccgagaag ccgaacgtgt tgctgctcga tgagccgacg aacgatctcg
     1441 acctcgacac cttgcgggca ctcgaggact tcctcgatga gtggccgggg gcgctcatcg
     1501 tggtcagcca cgaccgtgcc tttctcgagc gcacggtcgc cgacgtgatc gtcatggacg
     1561 gtaccggctt cgctggccgt cgtccgggcg ggtacgcggc ctacgaggag gagcgtcgag
     1621 ccgccccgag gcgccgaggt tcggtcgccg cagcgagagc cgccagtacg acgggtggag
     1681 ggggcaaggc gagagcgtcg agcgagacga aggcgagccg gtcatcgggc agcgccgaag
     1741 gtggggagaa actccgcagc ccctcgacgt tgcggcatcg gttccgagcg gtggaacgcg
     1801 agaccgagac gcttcaggcg gagcgtgatc ggatcaacgc cgaactggtg gaacatgcat
     1861 cggatcacgt gcgtctggca gggctggcgg ccgagttgca ggcggtcgtc gaggccctcg
     1921 aaccgatcga ggaggagtgg ctggagctgg ccgccgaggc cgaggaacga gggctcgacc
     1981 tcagctgaac cccgctgaac cgcgcccgcc ccgcccgccc cgcctgcccc gcctgttctc
     2041 cgataaccag tggtcgtgat cacgaccact ggttatcgga gaacaggcgt ctggggtggg
     2101 ctcaggtgtc ggggttggac tccttggccg cccaggagaa cacgcccttg gccatggtcg
     2161 atcgctcaac ctcggcccgg cgaccgaaga tcgcggcgcg ggcgcggcgc acctcgactg
     2221 aatccggcgc ggcgagcgag gccgtttcga tgaggtggcc ggcgaggcgg aactcgtccc
     2281 cttcagcgag ttcgagcgct cgctccacga ggcggtcagc gccgccggcg agcgccgcga
     2341 tctccgacgc gacggcagcg tcgggcgccg gtttcaggtt cgccgggttc ccgtcgtacc
     2401 agccgccgta gagacgccag atgttgcgaa cgatgaactc cggctcgtcg tagacgggtt
     2461 gcagccaggg ccgatcctcc agatcggtgg gtggccgaac ggtggccagg atgtcggtga
     2521 ggcgcgcccc ggcgttcatc atctcgatcg tctgatcgaa cagcgactcg agatagcggg
     2581 cggtgtcctc caacaccgtt cgaacccgtg cggctccggc gatcggcaac ccatggccgg
     2641 gaagcagata ctccggctcg agggccgcca tcgcccgaag cgcaaccgcc cactcgtcgc
     2701 agaagcgctg gaccttctgg gggtttccgc agttggggct ggcccagatg aacagatcgc
     2761 cggtgcacag caccttctcc tcgggaatcc acacccaggt cgaatcgtcg gtctcgccgc
     2821 gatcgtgacg cagttggaga tcgaccgatc ccaccgacgt cgaggtcgtc gaccggtagg
     2881 tctcgtccgg atagcggtag tcgcccggga aaaacggcgc gtcgagctgg aactgacgct
     2941 gattgatgac gccgttgtac ccgttggtga ggtcgtagcg acgaaagcga tcggcgacgg
     3001 cctcgtgtgc gatgaccttc gccgacccgt tgccctcggc ctcgaagagt ttcacggcat
     3061 agatgtggtc gacgtggccg tgggtgaaca ccgcggtgcg tagcggattg ggcgtccagc
     3121 gtcgggttgc gtcgacgatg cccggcgcat gcaacgggct gccggcatcg acgatgccga
     3181 gttcatcgcc gtcgcgaatg gcggtgacgt tggcgaatgt ctccatgaac gccacgcgat
     3241 ccgagatcgg ttccagggtc gtttctgagg cgatcgacac cgggtggtga gcggcgatcg
     3301 acaagcgccc gtcaaggatc ttctccgacg tctcgaacaa gctggtcatc gtgatacccc
     3361 cacgtcgcgt ggtagcggtg ccgcgacgac ggtgacccta gcgctgtggc tggacacgtg
     3421 tctggcctcg gaatcagtac ctgcgccgcc ggacgaacct gtgggccatc ttgcctaacg
     3481 caggccgaac ccttgagagc acggcgttcg aacggtacgg tggaaccggc tcgcgtctgt
     3541 ccctcaggct tgtgtgcggg cccgttctgg gagttggttg atgaagcaga cacatgcggc
     3601 cgtggcggga atcgttgcgg tgacggcgtc gttggcgttc gccggagtcg ccggcgctca
     3661 agacggagac ggggcgcaga ccgtcgagag caccaccgtc gagagcacca ccgtggagtc
     3721 cagcacgccc gataccacgg cacccgagca gtcgacgtcg tcgacgacgc ccgagtcgac
     3781 ggtgcccgag cctgcctccc cgatcgaggt gcccgccgat gaagcccctc cggccgatcc
     3841 cttcgaggtg cttcgcggag tcgatctccc aggtgtggcg gtgacctccg gaacgttgtc
     3901 gtttcaggtg tcgttttccg accagccggc ctcgagctgc ggtggcaacc tcgccatgtc
     3961 gggcggcggg atgaacgtcg cgtacgacga agctggcgga gtgggcggag cctacggact
     4021 ggtggagacc gggtctggca cgttcggcac cctgttcgtc gggttcggtc cgattccggt
     4081 cggcgtcggc atcatcagcg tcagcgacga gaattgcgaa ttcgacgccg tcggcatcgg
     4141 gacctactcg gcctcggctt cgacagcgga actcgcgggt gttggaatcg ggatccaccc
     4201 gggcgcgtat gagacaggcg attacgtgag cgagttcacc atcaccgcag gcgtcggcgc
     4261 cacaggtggt gaggccacac tcgatgtcga tcggctcggc gcgttcctgt tgcgcgatcg
     4321 tcctggactg aatctggcgt tgccaggatc gccctcggtt cctgccaccc cgccaatgcc
     4381 tgagaccccg gctgaaaccc cggtcgtgcc gggcgagcct cagacgccca cgtcggccga
     4441 gccgccagcc accgcaccga ccgcacagta aatggtgggc gccgcttccc ggtgacccaa
     4501 cgtctcgacg ggccagggag cccgtgagcc gcacacacgt gcaggcccaa ttcggtttcg
     4561 cgttatggtg ccatctctat ggacttcgat gattcaccgg aacaggcgga gtttcgtcga
     4621 gaggcccgcg cctggctcga cgcgaacgcg gaggtgcgcg atggcgagtc atcgatgtgg
     4681 cgcgcgctcc gcccacagaa cgatgccgac gatcgctggg cgttgcagcg ggcgcggcag
     4741 tggcaggcga ccaagtacgg gggcggctac accgggttgc actggccggt tgaggttggt
     4801 ggtcgcgggc tgagccccca cattgcgggt gtgtttaagc aagaggagtc ccgctacgac
     4861 gttccggcga acgcgtttca ggtcggcgtg gacatggtcg ggcccacgtt gatccgccat
     4921 gcgtcgaagg atctgtgccg ccgccacctc gacacgacgc tgcgtggcga tgacttctgg
     4981 tgtcagctct tcagcgagcc cggggcgggt tcggatctgg ccggtctgac cacccgtgcg
     5041 tttcgagacg gagaccgctt catcgtcaac ggtcagaagg tctggacgac gaccgcccac
     5101 tgggcaaccc acggtcttct gttggcccgg accaatcctg aggcaccgaa acacaaaggg
     5161 atcaccgcct tcgtggtcga catgtcgacg ccgggaatcg acattcggcc gctacgtcaa
     5221 gccgacggcg cggagcactt caacgaggtg ttcctcaccg acgtggagat ccccgccgac
     5281 aacgtcgtcg gagcggttga cgatggctgg gcggtggccc acacgatgtt gtcgagtgaa
     5341 cggtcggcga tcgctggtgg gggcatggtc cagttcgacg agatcttgat gctggcgcga
     5401 gaccgcgacc gcggcgagga cccgctcgtt cgacaggacc tcgcgcgcct gtattcatat
     5461 ttcgagatct ctcgattcct cggctatcgg gtgcagacgg cgctgagcca cggacgcaca
     5521 cctggcccgg agagttcggt gatgaagttg cacatcagcc gccaatacga attcggcgga
     5581 gatgtgatga tgtcgattct cggagctgcc gccatgtcct ggggcgacag cgctccgttc
     5641 ggcgggttct tccaaggggt tttcatggcg cagtgggcgc cgagaatcgg gggcggaacg
     5701 gaccagattc aacgcaacat cgtcggtgag cgagtactcg gactggcggg ggagccgcgt
     5761 cgcgatcgtt gagaggcgcc cgagaggcgc cccagatgcg gtcggcgaag cgctacagca
     5821 acccgagcgc aacggcggac acgacgagtg ccatcgttcc gacaaagtcg accgaggcgg
     5881 tgaccaacgg tgtcccgtag gtatcggggt cgacgttgaa gcgccacgct ccgacggttg
     5941 agtagtacgc gagggtggcg acgaacgcga gggttgcgac cgacgccagg caactcgtga
     6001 gcaacaacca ccagaagcca ggcgcagcag cgtggccgga aaggttggtt gcgagccagg
     6061 ctccgaacac gttgaacacg agcagcggca cggcgagccc gaagatggag gaggcatcga
     6121 gtctggcctg tgtgccgggc atcaacgtcg gtgagacgag gccgatgtgg aggttggtgg
     6181 cgatgcggcc acagagaatc ccgccgaggg ctcccgccga tgacacgaac gccggctgca
     6241 gaacgccgat ggcgggaacg atggccagca ggttctcctg gcgctgcaac acgacgccgg
     6301 cgaacgcgct caggatcagt gcgaaaaaca acaccggaag cgactcgcga aacacctgac
     6361 gggtcaggtc gagcgccgag cgccatgtcc agatgccgat aacaaccgcg gaaacgatga
     6421 tgagaagtcc caggaacgag tcgacgttgt ggccaccgac gagttgtgcg gcgagccaca
     6481 acgccggaat cgtgatgccg tcgccgaggg tcgacaccgt cggcgcgacg aggtagtcga
     6541 ggtcccactc gtaacgaacg gcaccgatgg tcaacaggac ggtcgcggcg gcgacgacga
     6601 tcgacccgag cagtccaccg atacccgaca ccatcgtgag ctcgagcatc gaaatgtcct
     6661 gcgatgtacc gagggccagc ccgagcacct tcgcaatcgc ggccagcacg acgctcatca
     6721 ccagcgtcag gctgaacgac accaacaggt tctggccgag tacggaatcc cgcttgaacg
     6781 acacgcggaa ggtgccggtg tggatcgcgg tcgacagacg gttgccgagg gttgaaaaca
     6841 cgttgccgcg aagcccgatt gcggccggaa ccatgatcaa aagcggtgac aggttgtgcc
     6901 aggtgtcctg aaagccgacg agcatcgcgc cggcggcgaa gcttgtcgtc gagttcaggg
     6961 cgagcgcgga gacgctttgg cgcgcggcgc ccccggtcgg accgagtagg tcgaccagcc
     7021 gccgggcgag gcgatcaggg cgcaacgccc ccagtgatcc gtcggaacgc gccatggtgc
     7081 cgcgatgacg ttagacccgg cgcgccgacc gatcgcgcca gcggcgcttc gtcagcgcca
     7141 agtcagcacg gtggcgcgct cggcattgga ggcggacaga aacagcgaac ggccaggaga
     7201 tgctcgcggg gctcgaaact cgacgacggt ggtgtcgtcg gccgctgctg gagggaggcg
     7261 acacgcgacg gcatcgccgg gttcggcagc ggcacacgac gtcccgtcct cgccggcacc
     7321 gtcggtcgct ccgctcaatt cgacagaatc tgcggcctgg gtggccgatg gcgtctgcag
     7381 acgaaccgag acggttgcgg cgtcgctcga cgctgactcg atgaagccga cgacgttcca
     7441 cgggggccgc tggatcacct cgacggccgc agggtaacgc tgctgccatc cggcgtcggc
     7501 agttccgaac accccg
//
LOCUS       DAOOMA010000040         7499 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_11636_length_7499_cov_3.557227, whole genome shotgun sequence.
ACCESSION   DAOOMA010000040 DAOOMA010000000
VERSION     DAOOMA010000040.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 7499)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7499)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7499
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11636_length_7499_cov_3.557227"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..532
                     /gene="tsaE"
                     /locus_tag="PLP95_02070"
     CDS             <1..532
                     /gene="tsaE"
                     /locus_tag="PLP95_02070"
                     /inference="COORDINATES: protein motif:HMM:TIGR00150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HPB44617.1"
                     /translation="IAALAEPGDLILLTGDLGAGKTAFAKGFGRALGVAEAITSPTFT
                     LVREYSGRLKLYHLDVYRIEQIEEALELGLAELLDEGAVTLIEWADNILSELPADRLE
                     IVIELVDPAAGTDPATGTDPVAGAEVAGAEVDLDDCRSLRFHLQGPRWAARSRAIAEA
                     VAPWTRPEPTSNGEAS"
     gene            529..1227
                     /gene="tsaB"
                     /locus_tag="PLP95_02075"
     CDS             529..1227
                     /gene="tsaB"
                     /locus_tag="PLP95_02075"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HPB44618.1"
                     /translation="MIILGIDTATSQAGCALGSYEGVLALSQCAKGRRHAENLTPAIR
                     FICEQAQIELGEIGAIAVDLGPGLFTGLRVGIATGKAMAHALRVPVIGVASLDLVAFA
                     VRYSSRLIVSAIDARRSELYYAFYRQVPGGVQRLGDYQLGSPADLAADLAERRDEMLL
                     VGDGFQRYHDLFAGVDKAEMADAGFAHPSAASLVQLAHAKALREEFEPPASLEPIYLR
                     KPDAEINWVTRNDG"
     gene            1220..1759
                     /gene="rimI"
                     /locus_tag="PLP95_02080"
     CDS             1220..1759
                     /gene="rimI"
                     /locus_tag="PLP95_02080"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: protein motif:HMM:TIGR01575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HPB44619.1"
                     /translation="MADGHGGWPLRATGSLNDVAIVAMRPRHLGGVAAIENLTSTRPW
                     SEELFASELKQATSRCWVAVRSNNEVVGFACLMSTGFETHITNIAAAVDHRREGIATA
                     LMVTMMDATMAWGLDAVTLEVKAGNDAAQGLYRRFGFVAEGVRPKYYAETGEDAVIMW
                     CRGIDSDDYADRLAQICSG"
     gene            1843..2856
                     /gene="tsaD"
                     /locus_tag="PLP95_02085"
     CDS             1843..2856
                     /gene="tsaD"
                     /locus_tag="PLP95_02085"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HPB44620.1"
                     /translation="MSADLSPTAPILGIETSCDETAAAVVAGGRTVMSSVVSSQVDLH
                     ARFGGVVPEIASRAHLDLIVPIVAESVVSAGVAEADIAAVGATCGPGLIGSLLVGVSA
                     AKALALAWDVPFVAVNHLEAHLYASLLEDPDLEFPLVVLLVSGGHTMLIRMDGPGRYE
                     LLGQTIDDAAGEAFDKVARFLGLGYPGGPAVDRLAMVGDPEAIAFPRSMLNDGYDFSF
                     SGLKTSVINHVRKHPDVSAEDVCASFQQAVVDVLVTKARRAAREVGAKGLALGGGVAA
                     NSQLRESFLDVCVDEQIAGFLPSHEMCTDNAAMVAAAAWYQLSLEGPSSLDVGAYPNL
                     SLI"
     gene            3003..3290
                     /gene="groES"
                     /locus_tag="PLP95_02090"
     CDS             3003..3290
                     /gene="groES"
                     /locus_tag="PLP95_02090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015797912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HPB44621.1"
                     /translation="MNLQPLEDRIVVRPAEAEATTASGLVIPDTAKEKPQQGEVLAVG
                     EGRFSDEGERIPLDIKVGDKVVYSKYGGTEISHGGEDLLILAARDVLAIVK"
     gene            3308..4936
                     /gene="groL"
                     /locus_tag="PLP95_02095"
     CDS             3308..4936
                     /gene="groL"
                     /locus_tag="PLP95_02095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004585059.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HPB44622.1"
                     /translation="MAKILKFDDEARRALEAGVNKLADAVKVTIGPKGRNVVLDKKFG
                     APTITNDGVSIAREIELEDPFENMGAQLVKEVATKTNDVAGDGTTTATVLAQALVREG
                     LRNVAAGASPLGLKRGIEAAVAAAVESIHSQATSVDDDQGNIAQVASISAADSAIGSV
                     IAEAIAKVGKDGVVTVEESNTFGMELDFVEGMQFDKGYLSPYFVTDAERQEAVLNDPY
                     ILFVNGKITSVQDLVPVLEKVMQSGKPLLIVAEDVEGEALATLVVNKIRGTFNSVAVK
                     APGFGDRRKAMLADMAILTGAQVISEEVGLKLDAADLSLLGRARKIVITKDETTIVEG
                     AGSQDDVNGRVAQIKAEIDNTDSDWDREKLQERLAKLSGGVAVVKVGAATEVELKEKK
                     HRIEDALSATRAAIEEGVVAGGGTALLRARAGLADVIAGLEGDEATGAKAVHRALEAP
                     TRQIAENAGLEGAVVVQRVEAEAGSVGLNAATGAFEDLLKAGVIDPAKVTRAALQNAA
                     SIAGLLLTTECLVADKPEEAAPAMPDMGGMGGMM"
     gene            5217..5957
                     /locus_tag="PLP95_02100"
     CDS             5217..5957
                     /locus_tag="PLP95_02100"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF014453.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006486 - protein glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HPB44623.1"
                     /translation="MEPTPMLDPRRRAKGVTLAPPPGTRSPGLTDERSPLRALPALDD
                     RRRGLYERFGKRAFDVVGASAIIVALSPVLLIEWLVLRIVLGPNVIITQERVGRNGEV
                     FGMYKFRTMHWSRREAHRPFAGPDRRVTHKADHDPRHTPIGRLFRKLSVDELPQLLNV
                     LNGDMSLVGPRPELATIVEEIHGHGHRRHSIRPGMTGEWQVTTRQTGKLLHECFDEDL
                     PYLERITLRNDLSILWDTVAVVSGQGGR"
     gene            6085..7194
                     /locus_tag="PLP95_02105"
     CDS             6085..7194
                     /locus_tag="PLP95_02105"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HPB44624.1"
                     /translation="MSPSADGYGSDRALVGIAPIFAERYDVTVVAAADGPTVAMLREA
                     GIEVIIAPDFALRRKFATPTGIIPASIRVARTAALLRRLHRERNFAGVYVNTVANMIL
                     PFIRTVVPAPVVVHVREVPRAGDRQNRLIFSQVNRVASLALSNSSHTAAFVRRVEPRL
                     TDRIEVVCGGVDDPGVVGRRPDASTPPLEIVCVGRLHPQKGQAVLLDALGPQIAQGAD
                     YRIHFWGDALAEHAHIEAALHEQVERYGIADRVVWHGYSSDITAMYTGMDLAVMPSTW
                     PEGFSLVTVEAQAAGLPVIATQPGGPTDIVIDGETGRLVGLVDPDGIRQAVEEMSDPN
                     VRERYSAAGRARYLDRFTTERSARGVAEAVTRLVG"
     gene            complement(7175..>7499)
                     /locus_tag="PLP95_02110"
     CDS             complement(7175..>7499)
                     /locus_tag="PLP95_02110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB44625.1"
                     /translation="LTASWSIYAVAYAANVPVANALTALLYSKQVLVGRVIDSAFIVV
                     MSIVVVHLDGVRYVPLVMTAGILVGTALPVLSLKRKGIFDDPAHIAPAELAPAPSAKL
                     SPQAG"
BASE COUNT         1232 a         2461 c         2559 g         1247 t
ORIGIN      
        1 gatcgccgcg ctcgccgaac cgggcgacct catcttgctg accggcgatc tcggtgccgg
       61 caagacggcc ttcgccaagg ggttcggccg ggcgctcggg gtggccgagg cgatcacctc
      121 gccgaccttc acgctggtgc gcgaatactc gggccggctg aagctgtatc acctcgacgt
      181 ctatcgcatc gagcagatcg aggaggcgct cgaactggga ctggccgaac tgctcgacga
      241 gggggcggtg accctcatcg aatgggccga caacatcttg tccgaactgc ccgcggatcg
      301 cttggagatc gtcatcgaac tcgtcgaccc ggccgcaggc accgacccgg ccacaggcac
      361 cgacccggtc gcaggcgccg aggtcgcagg cgccgaggtc gatctcgacg actgtcgcag
      421 cctgcggttc catctgcagg ggccccgctg ggcggcgcgc tcgcgtgcca tcgccgaggc
      481 cgtcgcaccg tggacccgcc ccgaaccgac ctcgaatggt gaggcgtcat gatcatcctc
      541 ggaatcgaca ccgcgacatc tcaggccggc tgcgcgctcg gaagctatga aggcgtcctg
      601 gcgctgtctc agtgcgcgaa gggtcgccgc cacgccgaga acctcacccc ggcgatccgc
      661 ttcatctgcg aacaggctca gatcgaactg ggcgagatcg gtgcgatcgc ggtcgacctc
      721 ggccccgggt tgttcaccgg gctccgcgtc ggcatagcga ccggcaaggc gatggcccat
      781 gccctgcggg ttccggtgat cggtgtcgcc agcctcgacc tggtggcctt cgcagtccgg
      841 tattcgtcgc gcctcatcgt gtcggcgatc gacgcccgcc gctcggagtt gtactacgcg
      901 ttctaccggc aggtgcccgg cggggtgcag cgcctcggcg actatcagct cggctctccg
      961 gccgacctcg ctgccgatct ggcggagcgg cgcgacgaga tgctgctcgt cggcgacggg
     1021 ttccagcgct atcacgacct cttcgccggg gtcgacaagg ccgagatggc cgacgccggg
     1081 ttcgcccacc cgagcgcggc gtcgctcgtg caactcgccc atgcgaaggc gttgcgcgag
     1141 gaattcgaac cccccgcgtc gctcgagccg atctacttgc gcaagcccga cgccgagatc
     1201 aattgggtca cccgcaacga tggctgacgg ccacggtggg tggccgttgc gtgcgacggg
     1261 gtcgctcaac gacgtcgcga tcgtggcgat gaggccccgc cacctcggcg gggtggcggc
     1321 catcgagaac ctgacgagca cgcggccgtg gtccgaagag ctgttcgcat cggagctcaa
     1381 acaggcgacg tcgcgttgtt gggtggcggt gcgatccaac aacgaggtgg tcgggttcgc
     1441 gtgtctgatg tcgaccgggt tcgaaaccca catcaccaac atcgcggcgg ccgtcgatca
     1501 tcggcgtgag ggaatcgcca ccgcgctcat ggtgacgatg atggatgcca cgatggcgtg
     1561 gggcctcgac gcggtcaccc tcgaggtgaa ggccggcaac gacgccgcgc aggggctgta
     1621 tcggcgtttc ggtttcgtgg ccgagggcgt gcggccgaag tactacgccg agaccggcga
     1681 ggacgcggtg atcatgtggt gccgtgggat cgattccgac gactacgcgg atcggctcgc
     1741 gcagatctgt tccggctgag acccgtgtgg gccgagaccc gtgccggctg agacctgtgc
     1801 tggctgagac ctcggtgtgc ggcgcccggt aacgtgatga cgatgagcgc cgacctttcg
     1861 ccgaccgcac cgattctcgg aatcgagacg agctgtgacg agaccgcggc cgcggtcgtc
     1921 gccggcggcc gaacggtgat gagttcggtc gtgtcgagcc aggtcgacct gcacgccagg
     1981 ttcggcggag tggtgcccga gatcgccagc cgggcccatc tcgatctcat cgtgccgatc
     2041 gtggccgaat ccgtggtttc ggccggggtc gccgaagccg acatcgcggc ggtgggggcc
     2101 acgtgtgggc cagggctgat cgggtcgttg ctggtcgggg tgagcgccgc caaggcgttg
     2161 gcccttgcgt gggacgtgcc cttcgtggcg gtgaatcacc tcgaggcgca cctgtacgcc
     2221 tcgctgctcg aggaccccga tctcgagttt ccgctcgtgg tgttgttggt gtcgggcggc
     2281 cacacgatgc tgatccgcat ggacggaccc gggcgctatg agttgctcgg ccagacgatc
     2341 gacgacgcgg cgggggaggc cttcgacaag gtcgcccggt tcctcggtct gggctatccg
     2401 ggcgggccgg cggtcgaccg gctggcgatg gtcggcgacc ccgaggcgat tgcgtttccc
     2461 cgctcgatgc tcaacgacgg ctacgacttc agcttctcgg gcctcaagac ctcggtcatc
     2521 aaccacgtgc gaaagcatcc cgacgtgagc gccgaggatg tgtgcgcatc gttccagcag
     2581 gcggtggtcg acgtgttggt gaccaaagcg cgtcgggccg cccgcgaggt cggggcgaag
     2641 gggttggcgc tcggcggggg tgtggcggcc aacagccagt tacgtgaatc cttcctcgac
     2701 gtgtgcgtgg acgagcagat cgccgggttc ttgccgagcc atgagatgtg caccgacaac
     2761 gcggcgatgg tggcggcggc ggcctggtat cagctgtccc tggaaggtcc gtcgagcctg
     2821 gatgtcggcg cctacccgaa tctctcgctg atctgacgtc gcggcgagcc gacgcggcgg
     2881 cgatctgagg gggcgcttag cactcgatca tgtggagtgc taacttggca ctcgcgccgg
     2941 ttgagtgcca acacggtcgc attgaagtcc gcaactagcg aaagcccaac ggaggcatct
     3001 gcatgaacct gcagccactc gaagaccgaa tcgtcgttcg tcccgccgag gcggaggcga
     3061 ccaccgcgag cggtctggtc atccccgaca ccgccaagga aaagccgcag cagggcgagg
     3121 ttctcgccgt cggcgaaggc cgcttcagcg acgaaggcga gcggatcccg ctcgacatca
     3181 aggtcggcga caaggtcgtg tactcgaagt acggcggcac cgagatttcc catggcggcg
     3241 aagacctgct catcctcgcc gcccgcgacg tgctcgcgat cgtcaagtaa ccgaaggacg
     3301 cagtcacatg gcaaagattc tgaaattcga cgacgaggcg cgccgtgccc tcgaggcggg
     3361 cgtgaacaag ctcgccgacg cggtcaaggt caccatcggc cccaagggtc gcaacgtcgt
     3421 gctcgacaag aagttcggtg cgccgacgat caccaacgac ggcgtgtcca tcgcccgtga
     3481 gatcgaactc gaagatccct tcgagaacat gggtgcccag ctcgtcaagg aagttgcgac
     3541 caagaccaac gacgtcgccg gcgacggaac caccaccgcc accgtgctcg cccaggccct
     3601 cgttcgtgaa ggcctccgca acgtcgccgc cggcgccagc ccgctcgggc tcaagcgcgg
     3661 catcgaggcc gccgtcgccg cagcggtcga gtcgatccac tcccaggcca cctcggtgga
     3721 cgacgatcag ggcaacatcg cccaggtcgc ctcgatctcg gccgccgatt cggcgatcgg
     3781 aagcgtcatc gccgaggcga tcgccaaggt cggcaaggac ggcgtggtca ccgtggaaga
     3841 gtcgaacacc ttcggcatgg aactcgactt cgtcgaaggc atgcagttcg acaagggcta
     3901 cctgtcgccg tacttcgtca ccgacgccga gcgtcaagaa gcggtgctga atgaccccta
     3961 catcctgttc gtcaacggca agatcacgtc ggtgcaggac ctcgttccgg tgctcgagaa
     4021 ggtcatgcag tcgggtaagc cgctgttgat cgtcgcggag gacgtcgagg gcgaggcgct
     4081 cgccaccctc gtggtcaaca agatccgtgg caccttcaac tcggtcgccg tcaaggcccc
     4141 cggcttcggc gaccgccgca aggcgatgct cgccgacatg gcgatcctca ccggtgccca
     4201 ggtcatcagc gaagaggtcg gcctcaagct cgatgccgcc gatctgtcgc tgctcgggcg
     4261 tgcccgcaag atcgtgatca ccaaggacga gaccaccatc gtcgagggcg ctggttccca
     4321 ggacgacgtg aacggtcgcg tggcccagat caaggccgag atcgacaaca ccgactccga
     4381 ctgggaccgc gagaagctcc aggagcgcct cgccaagctc agcggcggcg ttgcggtcgt
     4441 caaggtcggc gctgcgaccg aggtcgagct caaggaaaag aagcaccgca tcgaagatgc
     4501 gctctcggcc acccgcgccg cgatcgagga aggcgttgtc gccggtggcg gtactgcgct
     4561 gcttcgtgcc cgcgccggcc tcgcggatgt gatcgccggt ctcgagggcg acgaggcgac
     4621 cggagccaag gccgtgcatc gcgcgctcga ggcgccgacc cgccagatcg ccgagaacgc
     4681 cggtctcgaa ggtgccgtcg tcgtgcagcg cgtcgaggcc gaagctggtt cggttgggct
     4741 caacgccgcc accggcgcat tcgaggacct gctcaaggcc ggcgtcatcg atccggccaa
     4801 ggtgacccgt gcggcgttgc agaacgcggc ctcgatcgcc ggcctgttgc tgaccaccga
     4861 gtgcctcgtc gccgacaagc ccgaagaggc tgcgccggcg atgcccgaca tgggtggcat
     4921 gggcggcatg atgtagaacc cgcgttgcgg caccgtcggg tgccgcaacg gtcggttaat
     4981 cgtcaaacgg ggtggggtcg agtcgtcggc cccaccccgt cgtcgttttt ctgggtcgtc
     5041 gtttttctgg ttggcaatcg tcgctcggtg tgtcacggtg acctatgtcg cactggtcaa
     5101 gatgactcat ggtttcgtga gacgacgtct ggtttgctgg acgccggcca cgaatctcac
     5161 attgtctcgg tggccggata cgcatcatga ctcttggcga attcgcaacc tcttccgtgg
     5221 aacccacccc aatgctcgat ccccgccggc gcgccaaagg cgtgacgttg gcaccgccgc
     5281 cgggaactcg ctcgcccggg ctcaccgacg agcggtcgcc attgcgggcg ttgccggcgc
     5341 tcgacgatcg gcgccgcggg ctgtatgaac gtttcggcaa gcgggccttc gacgtcgtcg
     5401 gcgccagcgc gatcatcgtc gccctgagcc cggtgctgtt gatcgaatgg ctcgtgttgc
     5461 ggatcgtgct cggcccgaac gtgatcatca cccaagagcg cgtcggccgc aacggcgagg
     5521 tgttcgggat gtacaagttc cgcacgatgc attggagccg tcgcgaggct catcgcccct
     5581 ttgccgggcc cgaccgtcgg gtcacccaca aggccgacca cgacccgcgc catacgccga
     5641 tcggacgcct gttccgcaag ctgagcgtcg atgaacttcc gcagctcctg aacgtgttga
     5701 acggcgacat gtcgctggtc gggccccgcc cggagttggc gacgatcgtc gaggagatcc
     5761 acggccacgg ccaccgtcgc cacagcatcc gccccgggat gaccggagaa tggcaggtga
     5821 cgacccgtca gaccggaaaa ctgctgcacg agtgcttcga cgaggacctg ccgtatctcg
     5881 aacgcatcac cctccgcaac gacctgtcga tcctgtggga caccgtcgcg gtggtctcgg
     5941 gtcaaggcgg ccgctgagcc gcgatcccca tcgcgcgcgg agcgcgcccc cgccgtattt
     6001 cagcagcgcg gcgatacact tccaacgtca actgcgagcc ttccccccgg agtttccgtg
     6061 tcccctcgtc gatcattgct cgtggtgtcc ccgtccgcgg acgggtacgg atctgaccgt
     6121 gcgctcgtcg gtatcgcgcc gattttcgcc gaacgctacg acgtcaccgt cgtcgctgcg
     6181 gcagatggcc cgaccgttgc gatgttgcgc gaggccggta tcgaggtgat catcgcaccc
     6241 gacttcgcgt tgcgacgaaa gttcgccacg ccgaccggga tcattccggc gtcgattcgc
     6301 gtcgccagaa cggccgcgtt gctccggcgg ttgcatcggg agcggaactt cgccggcgtc
     6361 tacgtcaaca cggtcgcgaa catgatcctt ccgttcatcc gcacggtggt tcccgctccg
     6421 gtcgtcgttc acgttcgcga ggtgccccgt gccggagatc gacagaatcg actcatcttc
     6481 agccaggtca accgggtagc gtcgttggcg ctgagcaact cgagtcatac cgcagcgttc
     6541 gttcgccgtg tggaaccgcg cctcaccgat cggatcgagg tggtctgcgg tggggtcgat
     6601 gacccgggcg tggtggggcg tcgacccgat gcgtccacgc ctccgctcga gatcgtgtgc
     6661 gtcggtcggt tgcaccccca aaaaggccag gccgtgctgt tggacgcgct cgggccgcag
     6721 atcgcgcagg gtgccgacta ccggattcat ttctggggtg acgcgctcgc agagcacgcg
     6781 cacatcgaag cggcgctgca cgaacaggtc gagcggtacg gcatcgccga tcgggtggtc
     6841 tggcacggct acagcagcga catcacggcg atgtacacgg gcatggacct cgcggtgatg
     6901 ccctcgacct ggccggaggg tttctcgctc gtcaccgtcg aagcccaagc cgccggcctg
     6961 ccggtcatcg ccactcaacc cgggggcccg accgacatcg tgatcgatgg ggagacgggc
     7021 cgtctcgtcg gactcgtcga ccccgacggg atccgccagg cggtcgagga gatgagcgat
     7081 ccgaacgtgc gcgagcggta ttcggcggcg ggccgcgctc gatatctcga ccggttcacc
     7141 accgaacgat cggctcgcgg ggtggccgag gccgtcaccc ggcttgtggg ctgagcttcg
     7201 ccgagggcgc cggggccaat tcggcggggg cgatgtgcgc cgggtcgtcg aagatgccct
     7261 tgcgcttcag tgacagcacg ggcagggcgg tgcccaccag aatgccggcg gtcatgacca
     7321 ggggcacgta gcggaccccg tcgagatgga ccacgacgat gctcatcacg acgatgaacg
     7381 ccgagtcgat cactcggccg acgagcacct gcttgctgta cagcagcgcg gtgagtgcgt
     7441 tggcgaccgg aacgttggcg gcgtaggcga cggcgtagat cgaccaggac gcggtgagg
//
LOCUS       DAOOMA010000041         7495 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_11644_length_7495_cov_4.856989, whole genome shotgun sequence.
ACCESSION   DAOOMA010000041 DAOOMA010000000
VERSION     DAOOMA010000041.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 7495)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7495)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7495
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11644_length_7495_cov_4.856989"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..408
                     /locus_tag="PLP95_02115"
     CDS             <1..408
                     /locus_tag="PLP95_02115"
                     /inference="COORDINATES: protein motif:HMM:NF017050.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC oxidoreductase"
                     /protein_id="HPB44626.1"
                     /translation="SPDTRVDPLIDPRYFSHPYDMETLIKGTQIALDIMNSAPFDPYR
                     GEMLIRHDRNDPHQIEQTLRQHADTEYHVCGTAKMGPDSDPMAVVDASLRVRGIEGMR
                     IADASVMPRVPSNNINAPVIMIGEKCADMIRTG"
     gene            complement(414..1019)
                     /locus_tag="PLP95_02120"
     CDS             complement(414..1019)
                     /locus_tag="PLP95_02120"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HPB44627.1"
                     /translation="MPRIKADTIAEHVDQQRAAVLDAAVELFTTRGFAEVSLGDVAAK
                     VGLARSSLYRYVPDKTHLLVEWYRRAVPDITSAWSAVTAGDDDPVVRLQRWGHAFLEW
                     ANSPEHQLVAPLMEAIPNLDAQTRAEISSLHRTMMGTVGEVLQAVGTPPEQIDGTVDL
                     LAGLLLSAARAEQAHGVNPQVRRRLDAAVAAIIAPLDNDDA"
     gene            complement(1030..1767)
                     /locus_tag="PLP95_02125"
     CDS             complement(1030..1767)
                     /locus_tag="PLP95_02125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPB44628.1"
                     /translation="MSLSSNTEPATSHPGNAPSALVVSKARVAYPDGPDVRVVLDDLD
                     LSVSPGEIVVISGESGAGKSTLLTIAGLLRRPDSGEVTVAGIPTSALSERQLTVVRRD
                     HLAFVYQSANLLPSLTAVEQLELVGHIRGEKPSTMRARALETLAAVDLADRANQLPSQ
                     LSGGERQRVGIARALMASPSVLIADEPTASLDPERAAVVAELLAGAAAANGIATIIVA
                     HDTAALAHADRHLRLEGGRLHEFARTV"
     gene            complement(1764..2855)
                     /locus_tag="PLP95_02130"
     CDS             complement(1764..2855)
                     /locus_tag="PLP95_02130"
                     /inference="COORDINATES: protein motif:HMM:NF014717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HPB44629.1"
                     /translation="MFLALRDLRRGSRRFALLGAVIALVAVLSTVLSGLATGLVTDGI
                     SGLRALPLDRLALQEGAQATFSRSALQPEVLEPFEQLDGVEVTPLGASFVNAAANNGG
                     LSLDLALFGIAPESFLVTRNDASDALHGEPGLILGSELADEGVKVGDRYTLVGSDIEL
                     PVLGFTFAGTYGHAPIAFTSLETWQRVQFGDSAKGRFSAVALKGGDPADINAAAKAAG
                     VEVITKSAAYAGSPGFTAETTTMSLIRSFLLVISALVIGAFFTVLTVQRTRQIGLLKA
                     MGASNAYVLRDGIGQMTILVALATAAGVLVGAALIALVGGSGAPVELNLAAIATVAGS
                     LVVAGVLGSLVAFRRITQIEPAIALGVES"
     gene            complement(2946..4394)
                     /locus_tag="PLP95_02135"
     CDS             complement(2946..4394)
                     /locus_tag="PLP95_02135"
                     /inference="COORDINATES: protein motif:HMM:NF013086.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HPB44630.1"
                     /translation="MTDADVDPIQPLDVSELSDGWAEECDVLVIGLGAAGLASSIAAA
                     EAGASVIALDRSGGGGTSASAGGILYLGGGTSTQVEAGLSDTAEEMRAFLAEALGRDI
                     DDPRLVAYCEGSVEHHDWLVAHGVPFEPAFWDEPGMEPPGTEGLIYSGGEDASPYRQH
                     HRPVARGHVPATPNAAGWFLVERLREALERTAATVLTDQRAERLVVDGGRVVGATVRA
                     DGTVRAIRANSGVVLACGGWAYNEALLAKYAPKLLDIAWRLGTDGDDGWALRTTLGLG
                     AEIEGMSTMEVAVPVTPPRSVVRGVIVNGEGERFINEDTYFGHVGQAILRDQDGVAWL
                     LTDEPNYVVNRVGMRAENVCETWEELAGEIGLPPEKLAATMAAYNEAAARGQDPQFHK
                     AAEWLVPLNTAPFGAIDLRVATILYAGFPLGGTRIDEHARVVRPDGSPIDGLFAAGRA
                     ASSLALAHYCSGISLGEGTFFGRRAVAEMFST"
     gene            4424..5704
                     /locus_tag="PLP95_02140"
     CDS             4424..5704
                     /locus_tag="PLP95_02140"
                     /inference="COORDINATES: protein motif:HMM:NF012296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HPB44631.1"
                     /translation="MQVRTVPADALDKVGRTAQDVAMFDGTDEQPTFLPRSGESWRSP
                     WEMYARLRDRDPAHHVVGGDYWVLSRFDDVMNAARDTTTFSSAEGLTFTYGERDKVGL
                     DQVRPMVMMDPPEHTAFRRLVGRGFTPRHVAAIEPEVRRFVVERIERLRTTGTGDVVE
                     ELFKPLPSLVVASYLGVPDADRDRFDAWTDAIVAANSNGNPLDAGEAVGGLFTYFSEL
                     IETRRRRPGDDTISALVATGAAEVPMLQVLGFAFTMVTGGNDTTTGLLGTAAELLTKH
                     PDQRARVIADPSLVEPAIEELLRLSAPVQGLARTTTRPVELHGRTIPAAKKVMLLYGS
                     ANRDPREFGPTAEAFDVGRSIKRIMTFSYGAHHCLGAAAARLQARVVLEELLARCPNF
                     AVDSDAGEFAAGHFVRRYKSLPFVAEASVGRVRA"
     gene            5701..6750
                     /locus_tag="PLP95_02145"
     CDS             5701..6750
                     /locus_tag="PLP95_02145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase C-terminal
                     domain-containing protein"
                     /protein_id="HPB44632.1"
                     /translation="MSDDATTPSNSRPGSVQRGLADETGEFVRDTAYIEDRITRDGRD
                     GWPVEAGRYRLVVARACPWANRAIIVRRLLGLESVLSMGIAGPTHDERSWQFDLDPDG
                     LDPVLGIERLKEAFEKRFPGYPKGITVPAIVDIPSGQVVTNNYPQITLDLSSEWTEFH
                     REGAPDLYPEPLRDEMDEVMELVYRDVNNGVYAAGFARSQESYERAYRRLFDRLDWLS
                     ERLATRRFLMGDTITEADVRLFTTLARFDPVYHGHFKCNRSKLSEMSVLWAYARDLFQ
                     TPGFGDTTDFQQIKEHYYVVHTSINPTQIVPLGPDLANWMTPHGREQLGGRPFGDGTP
                     PGPPLPSERVTAAIG"
     gene            6797..>7495
                     /locus_tag="PLP95_02150"
     CDS             6797..>7495
                     /locus_tag="PLP95_02150"
                     /inference="COORDINATES: protein motif:HMM:NF019845.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HPB44633.1"
                     /translation="MYAVTIVDGALEWREHPDPVPGTGEVLVRVRAAGLNSADRLQLK
                     GFYPAPPGSPADIPGLELAGEVAALGPGAERFSVGERVMAVVGGGAQAELCVIHERHL
                     LAVPDGVDWSAAGGFAEAFTTAHDALFTQAALSVGERMCVHGAAGGVGIAAVQLGVAA
                     GAQVVATVRDERLRDRVAAFGAEAVAPDGFGAHGPFDVILELIGGPNMAENLRSLATG
                     GRVSVIGVGGGPKVE"
BASE COUNT         1205 a         2472 c         2605 g         1213 t
ORIGIN      
        1 agccccgaca cccgcgtgga tccgctgatc gacccccgtt atttcagcca cccgtacgac
       61 atggagacgc tgatcaaggg cacccagatc gcactcgaca tcatgaacag cgcgccgttc
      121 gatccctacc gtggcgagat gctgatccgc catgaccgca acgatccgca ccagatcgag
      181 cagaccctgc gccaacacgc cgataccgaa taccacgtgt gcggaacggc gaagatgggc
      241 ccggacagcg acccgatggc ggtggtcgac gcgtcgcttc gggtccgcgg catcgagggg
      301 atgcgaatcg cggatgcgtc ggtgatgccc cgggtcccga gcaacaacat caatgcgccg
      361 gtgatcatga tcggtgagaa gtgcgccgac atgatcagaa ccggctagct ggatcacgcg
      421 tcgtcgttgt ccagcggggc gatgatggcc gcgacggcgg cgtcgaggcg ccgtcgtacc
      481 tgcgggttca ccccgtgggc ctgttcggcc ctcgccgcgc tgagcagcag tccggcgagc
      541 agatcgaccg tgccgtcgat ctgctccggc ggtgttccga ccgcttgaag gacctcgccg
      601 acggttccca tcatcgtgcg gtgcaacgag ctgatctcgg ctcgggtctg cgcatcgagg
      661 ttcgggatgg cctccatgag cggagcgacg agttggtgtt cggggctgtt ggcccattcg
      721 aggaacgcgt gtccccaccg ttgcagtcgc accacgggat cgtcgtcacc cgcggtcacc
      781 gcgctccacg cactggtgat gtccggtacc gctcgccgat accactcgac gaggaggtgg
      841 gtcttgtcgg ggacatatcg atacagcgag ctgcgggcca ggccgacctt cgcggcgacg
      901 tctccgaggc tgacctcggc gaagccgcgg gtggtgaaca actcgacggc ggcgtcgagc
      961 accgcagcgc gctgctggtc gacgtgttcg gcgatggtgt cggctttgat ccgtggcacg
     1021 atgcaagcct cacacagtgc gggcgaattc gtggaggcgg cctccctcga ggcgcagatg
     1081 ccggtcggcg tgtgcgagcg ctgcggtgtc gtgggccacg atgatggtgg cgatgccatt
     1141 cgcggccgcc gcaccggcga gaagctcagc caccaccgca gcgcgctcgg ggtcgagcga
     1201 ggcggtcggc tcatcggcga tgaggacgct cggggaggcc atgagcgctc gggcgatgcc
     1261 cacccgctgg cgttcaccgc ccgacagctg cgagggcagt tggttggctc gatccgccag
     1321 gtcgaccgca gcgagtgtct cgagcgcgcg tgctcgcatg gtcgagggtt tctcgcctcg
     1381 gatgtggccg acgagttcga gttgttccac cgcggtgagg gatggcaaca ggttggccga
     1441 ctgatagacg aacgccaggt ggtcgcgacg tacgacggtg agttggcgct ccgagagcgc
     1501 cgaggtgggg attccggcga cggtcacctc gccggaatcg ggacggcgca gcaggccggc
     1561 gatcgtcaac agggtggact taccggcacc ggactcgccg gagatcacca cgatctcgcc
     1621 cggcgagacg ctcaggtcca ggtcgtcgag cacgacgcgc acgtcgggac catccgggta
     1681 ggcgactcgc gccttcgaca cgacgagcgc gcttggcgcg ttgccggggt gggaggtcgc
     1741 gggttcagtg ttcgaggaga ggctcatgat tcgactccga gggcgatggc cggttcgatt
     1801 tgggtgattc ggcggaaggc gacgaggctg ccgagaactc cggcgaccac gagcgatccg
     1861 gcgacggtgg cgatcgccgc caggttgagt tccacggggg cgccgctgcc gccgaccagg
     1921 gcgatcaggg cggcgccgac gagcaccccg gcggcggtgg cgagggcgac gaggatcgtc
     1981 atctggccga tgccgtctcg tagcacgtag gcgttgctgg ctcccatcgc cttgagcaac
     2041 ccgatctggc gggtccgctg cacggtgagc acggtgaaga acgccccgat gaccagcgcc
     2101 gaaatgacga gcaggaacga gcggatcagg ctcatcgttg tcgtctcggc ggtgaagccg
     2161 ggggatccgg cgtaagcggc ggacttggtg atgacctcga ctccggccgc cttggcggcc
     2221 gcgttaatgt cggccgggtc gccgcccttg agcgcgaccg ccgagaaccg gcccttggcg
     2281 ctgtctccga actgaacgcg ctgccacgtc tcgagcgagg tgaacgcgat cggtgcgtgt
     2341 ccgtaggtgc cggcgaaggt gaatcccaac acggggagtt cgatgtcgga tccgacgagg
     2401 gtgtagcgat cgccaacctt gacgccttca tcggccagtt cgctgcccag gatgaggccg
     2461 ggctcgccgt gcagcgcatc gctggcgtcg ttgcgtgtga ccaggaagct ttcgggggcg
     2521 atgccgaaga gggcgaggtc gaggctcagc ccgccgttat tcgccgccgc gttgacgaag
     2581 ctggccccga gcggcgtcac ctcgaccccg tcgagctgtt cgaacggctc gagtacctcg
     2641 ggttgcagcg cggagcggct gaaggttgcc tgggcgcctt cttggagcgc gagccgatcg
     2701 agcggcaacg cacgcagccc cgagatgccg tcggtgacca ggccggtggc cagtcccgac
     2761 agcaccgttg acagaaccgc taccagcgcg atgaccgcgc ccagcagcgc gaatcggcga
     2821 ctcccgcgcc gtaggtcacg tagtgcgaga aacatcacat ctccatcttg tcgacatcat
     2881 gtcagcatct tagcgacatg atgtcgacaa gatgtcggca agcttcattt caggttgaga
     2941 acctctcagg ttgaaaacat ctcggcaacg gcgcgccggc cgaaaaacgt gccctcgccg
     3001 agggagatgc ccgagcagta gtgggcgagc gccaggctcg atgcggcgcg gccggcggcg
     3061 aacaatccgt cgatggggga accgtcgggg cgaacgactc gagcgtgttc gtcgatccgg
     3121 gtgccgccga ggggaaaccc ggcgtagaga atggtcgcga cccgcaggtc gatcgccccg
     3181 aacggcgccg tgttgagcgg cacgagccac tcggcggcct tgtggaactg cgggtcctgc
     3241 ccgcgagcgg ccgcctcgtt gtaggccgcc atcgttgcgg cgagcttttc gggcggcagc
     3301 ccgatctcgc cagccaactc ctcccaggtc tcacacacgt tctcggcgcg catgccaacc
     3361 cggttgacca cgtagttggg ttcgtcggtg agcagccacg caaccccgtc ctggtcgcgc
     3421 aagatggcct ggccgacatg tccgaaatag gtgtcctcgt tgatgaagcg ttcgccctca
     3481 ccgttgacga tcaccccgcg aaccaccgag cgcggcgggg tcaccggtac ggcgacctcc
     3541 atcgtcgaca ttccctcgat ctcggcgccg agcccgagtg tggttcgcag cgcccacccg
     3601 tcgtcgccgt cggtgccgag tcgccacgcg atgtcgagca gcttcggcgc atatttcgcg
     3661 agcagcgcct cgttgtaggc ccagccgccg caggcgagga ccacgccgct gttcgcccgg
     3721 atggcgcgaa ccgtgccatc ggcgcgcacc gtggccccga cgacccggcc cccgtccacg
     3781 acgagccgtt ccgcgcgctg atcggtcagc acggtggccg ccgtgcgttc gagcgcctcg
     3841 cggagacgtt ccaccaggaa ccaaccggca gcgttcggcg ttgcggggac gtggccgcgc
     3901 gccaccggtc gatgatgttg gcgatacggg ctggcgtcct cgcctccgct gtagatcagg
     3961 ccctcggtgc cgggcggctc catgccgggc tcgtcccaga atgcgggctc gaacgggacc
     4021 ccgtgggcga ccaaccagtc gtggtgttcg acgctgccct cgcagtacgc gaccaggcgc
     4081 ggatcgtcga tgtcgcggcc gagcgcctcg gccaggaacg cgcgcatctc ctcggcggtg
     4141 tcgctcaggc cggcctcgac ctgggtcgag gtaccgccgc cgaggtagag gattccgccc
     4201 gcactcgccg aggttccgcc accgccgctt cggtcgaggg cgatgaccga cgccccggcc
     4261 tcggccgcag cgatcgagga cgccagcccc gcagcgccga ggccgatgac gagcacgtcg
     4321 cattcctcgg cccagccgtc ggacaactcc gacacgtcga gtggttggat cgggtctaca
     4381 tctgcatcag tcacgtcgcg cttctaggac agggggtcac gcggtgcaag tccgaacagt
     4441 gccggccgat gcgctcgaca aggtcggccg aaccgcacag gatgtggcga tgttcgatgg
     4501 caccgacgag cagccgacgt tccttccgcg atcgggcgag tcctggcgtt caccgtggga
     4561 gatgtacgcc cggctccgcg atcgcgaccc tgcccaccac gtcgtcggtg gcgattactg
     4621 ggtcttgtcg cgattcgacg acgtgatgaa cgccgcccga gacacgacga cgttctcctc
     4681 ggccgagggg ctgaccttca cctacggcga acgggacaag gtcggcctcg accaggttcg
     4741 tccgatggtc atgatggacc cgccggagca cacggcgttt cgtcgtctgg tcggccgagg
     4801 gttcaccccg cgccacgtgg ccgcgatcga gccggaggtg cgccgattcg tggtggagcg
     4861 gatcgagcgc ctgagaacaa cgggcaccgg cgatgtggtg gaggagttgt tcaaaccgct
     4921 tccgagtctc gtcgtcgcct cctacctggg cgtgcccgat gcggaccggg accgcttcga
     4981 cgcctggacc gatgcgatcg tcgccgcgaa ctcgaacggc aacccgctcg acgccggcga
     5041 agcggtaggg ggcctgttca cctacttcag cgagctcatc gagaccaggc gacgccggcc
     5101 gggcgacgac accatttcgg cgttggtcgc cacgggcgcc gctgaggttc caatgctgca
     5161 ggtgctcggc tttgcgttca ccatggtcac cggcggcaac gacaccacga cgggactgtt
     5221 gggaaccgcg gcggaactcc tcacgaagca tcccgaccag cgcgctcgtg tcatcgccga
     5281 tccctcgttg gtcgagccgg ccatcgagga gctcctgcgg ctgtcggccc cggttcaggg
     5341 actcgcccga accacgaccc ggccggtgga gctgcacggt cggacgatcc ccgcggccaa
     5401 gaaggtgatg ctgttgtacg ggtcggccaa ccgtgaccct cgcgagttcg ggccgaccgc
     5461 ggaggccttc gacgtcggcc ggtccatcaa gcggatcatg acgttctcct atggggctca
     5521 tcactgcctt ggcgctgcgg cggcccggct gcaggcgcgg gtcgtgctcg aggagctgtt
     5581 ggcgcggtgc ccgaacttcg cagtcgacag cgatgccggg gagttcgccg caggtcactt
     5641 cgtgcggcga tacaagtcgc ttcccttcgt cgccgaagcg tcggtgggta gggtgcgtgc
     5701 atgagcgacg acgcgaccac gccatcgaat tcacgtccgg ggtcggtgca acggggcctt
     5761 gccgatgaga ccggcgaatt cgtccgcgac accgcctaca tcgaagaccg catcacccgc
     5821 gacgggcgcg acggctggcc cgtcgaggcg ggacgatacc gcctcgtcgt cgctcgggcg
     5881 tgtccgtggg cgaaccgggc gatcatcgtg cggcgcctgc tcggactcga aagcgtcttg
     5941 agcatgggca tcgccggccc cacccacgac gaacgcagct ggcaattcga cctcgatccc
     6001 gacgggctcg atccggttct gggcatcgag cgcctcaagg aggcgttcga gaaacgcttt
     6061 cccggttatc ccaaggggat caccgttccc gcgatcgtcg acatcccctc ggggcaggtc
     6121 gtgaccaaca actacccgca gatcaccctc gacctgtcga gcgaatggac cgagtttcat
     6181 cgcgagggcg cgccggacct gtacccggaa ccgctgcgtg acgagatgga tgaggtcatg
     6241 gaactcgtct accgcgacgt gaacaacggg gtctatgccg ccgggtttgc tcgcagccag
     6301 gaatcctacg agcgggccta tcgacggctg ttcgaccggc tcgactggct gagcgaacgc
     6361 ctcgccaccc gacggtttct gatgggagac accatcaccg aggcagacgt tcggttgttc
     6421 acgacgctcg cccgcttcga ccccgtctat cacggacatt tcaaatgcaa ccgatcgaag
     6481 ttgagcgaga tgtccgtgtt gtgggcctac gcccgcgacc tcttccagac tcccgggttc
     6541 ggcgacacca ccgacttcca gcagatcaag gagcactatt acgtggtgca cacctcgatc
     6601 aacccgaccc agatcgtccc gctcggccct gatctcgcga actggatgac cccgcatgga
     6661 cgtgagcaac tgggcggccg acccttcggc gacggcaccc cacccggtcc gccgttgccg
     6721 tcggagcggg tgaccgctgc gatcggctga acggccgaca tccacacgcc ccaacggccc
     6781 gggaggtcaa cgaaatatgt atgcggttac gatcgtcgac ggcgcgctgg aatggcgcga
     6841 acaccccgac ccggttccgg ggaccggtga ggtgctggtc cgggtgcgag cagcggggct
     6901 caacagcgcg gatcggctgc agttgaaggg cttctaccct gcgccaccgg gttcgcccgc
     6961 cgacatcccg ggcctggagc tggccggtga ggttgcggcg ctcgggccgg gcgccgagcg
     7021 gttctccgtg ggggaacggg tgatggccgt cgtcggcggt ggcgctcagg cggagctgtg
     7081 cgtgattcat gaacgccacc tgttggcggt tcccgatggg gtcgactgga gtgcggccgg
     7141 cggctttgcg gaggcgttca ccacggcgca cgacgccctg ttcacccagg cggcgttgtc
     7201 ggtcggcgag cggatgtgcg tgcacggcgc cgccggaggg gtggggatcg cagccgttca
     7261 actcggtgtg gccgccggcg cgcaggtcgt cgcaacggtg cgcgatgagc gcctgcgcga
     7321 ccgggtcgcc gcattcggcg cggaagcggt cgcacccgat gggttcggcg cccacggccc
     7381 cttcgacgtg atcctcgagc tgatcggcgg cccgaacatg gccgagaacc tgcggtcgct
     7441 cgccaccggc ggccgggtct cggttatcgg agtcggtggt ggtccgaagg tcgag
//
LOCUS       DAOOMA010000042         7460 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_11729_length_7460_cov_3.765159, whole genome shotgun sequence.
ACCESSION   DAOOMA010000042 DAOOMA010000000
VERSION     DAOOMA010000042.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 7460)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7460)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7460
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11729_length_7460_cov_3.765159"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1590
                     /gene="infB"
                     /locus_tag="PLP95_02155"
     CDS             <1..1590
                     /gene="infB"
                     /locus_tag="PLP95_02155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HPB44634.1"
                     /translation="EQEVELQKVLDIPEDDEDDLSEYPHRAPVITVMGHVDHGKTKLL
                     DRIRNANVQAGEAGGITQHIGAYQVIKNGKPITFLDTPGHEAFTAMRVRGAEATDIVI
                     LVVAADDGVMPQTIEAIQHAKAAEVPIVVAVNKIDREGADPQKVMSQVAEHGLVPEAW
                     GGDTVFCEISALQEIGIDDLLDNLLLVAEVEDLRADPEGRATGVVLESHLDVGRGPVA
                     TVLVQRGTLRVGDPLVAGPAWGRVRALINDHGEQVKEAGPSTPVRVLGLSDVADSGDD
                     FVVAPDERKAKSVAQTREHWQRDARRGRDSSVMSGGARLEDIFDQIQRGESATLNMIV
                     KADTSGSLEALIESLRKLERPEVKIGFVLRGVGGITESDIQLAETSDATVVGFNVRPD
                     RRVREFAEAEGVEIRTYDVIYKLLEDVEAAMVGMLEPEFEEVVTGDAEVREIFKVPKI
                     GFIAGCYVLNGKITRNSRVRFIREGTVIWKGSVQSLRRFKEDAREVAAGYECGIGLSD
                     YQDLRPGDIIETYDLVEIPRT"
     gene            1704..2144
                     /gene="rbfA"
                     /locus_tag="PLP95_02160"
     CDS             1704..2144
                     /gene="rbfA"
                     /locus_tag="PLP95_02160"
                     /inference="COORDINATES: protein motif:HMM:TIGR00082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HPB44635.1"
                     /translation="MARPRQRAQARHQFNRTDRIAETIREIVATELERIDDERADLVT
                     VTGVKVDNDLNTAKVYYSAIVAETEGRLDDVAEALNDARWPIQQVVNRSVRARKTPQI
                     QFLPDDVLAAALRIDDILNDRVHPAGVDSDADLPGSDGDPAAAG"
     gene            2161..2622
                     /locus_tag="PLP95_02165"
     CDS             2161..2622
                     /locus_tag="PLP95_02165"
                     /inference="COORDINATES: protein motif:HMM:NF013660.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB44636.1"
                     /translation="MGRRGKGGEGTVHGVAVVDKEAGWTSHDVVAKARGLLGTRRVGH
                     SGTLDPDATGVLVLGVGRATKLLRFLQLLPKSYRCEIIFGADTSTLDDSGEVVATYDM
                     AFSPQEAAAAAATFVGDIMQVPPMVSAVKVGGRRLHELAARGSRSNATPGR"
     gene            2628..3050
                     /locus_tag="PLP95_02170"
     CDS             2628..3050
                     /locus_tag="PLP95_02170"
                     /inference="COORDINATES: protein motif:HMM:NF027523.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB44637.1"
                     /translation="MRYDLEATADPLVYRAEIECSSGTYVRVLAEDLGRALGGGAHIR
                     RLRRTSVGPFTLDDARPLESIEALPMLEAVRGFDRVVVDEDTEAAVRVGAVLDSQRLG
                     VEPHDPGPWPVVSGSGELLAMYEPFRDGRVKPAVVLVG"
     gene            3062..4027
                     /locus_tag="PLP95_02175"
     CDS             3062..4027
                     /locus_tag="PLP95_02175"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: protein motif:HMM:NF004160.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HPB44638.1"
                     /translation="MHIARQLSDVVDPRAGNVVSIGFYDGVHLGHRALITSLLEMSSS
                     RGLATTVVTFDQHPARVLRPEVAPQLLCDLDQKLELLGETGVDQTLVIHFDEAQAAQP
                     AEDFVTSVLVDALQARVVVVGEDFHFGHRRLGNVALLTEMGETHGFEVIGHHLVGIDW
                     TDARPEQQVSSTAIRRAVVEGRIEDANAMLGRPHTIRGTVVGGDKRGRTIGFPTANVV
                     IHPELLQPADGIYVGELVRANAEVLPAAVYQGRRPTFYEDQPTSILEVHALDWAGDLY
                     GEPVQVQLHKRLRGDRKFDTIEELTAQLAIDCQRATEYLRERDRS"
     gene            4024..5142
                     /locus_tag="PLP95_02180"
     CDS             4024..5142
                     /locus_tag="PLP95_02180"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HPB44639.1"
                     /translation="MNAMAMNSSSTNSGVEPIPGPHPEGVVEDGAPVVAPRLSRRRIR
                     LDDGHEVGISVAGHGVPLVVVHGFSAEGFLYAQTLHRLVSMGFKVIAIDTAGHGGTRG
                     LPSDGANLRSYGELLARVIDKLGIERCILAGHSMGGRMVTDVAAMQPERTVAVMLIDA
                     IVGDTWDKMVYAFRVYPPLVGAIGTALAVDSLTILPLWSDPRQAIKFLRLALPTIIGD
                     VVRPWRLLGPVVSILRSRSSRYSLNALGDAGVPLYVLHGDRDLAVPMRTASDAAKRAN
                     GTLITVNGGGHSWLLRDPETLPSIVSQLMHGPLSEEITAAIRSAGMTSEEPTDAELDS
                     VFYRPDALIHELTPTREVTHVVGRHWRPTFRWTTSAPA"
     assembly_gap    5174..5273
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            5451..5729
                     /gene="rpsO"
                     /locus_tag="PLP95_02185"
     CDS             5451..5729
                     /gene="rpsO"
                     /locus_tag="PLP95_02185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012227663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HPB44640.1"
                     /translation="MSRPPANLPPKADTIAKHARGEGDTGSPEVQIALLTDRINHLTE
                     HLKQHKKDHHSRRGLLMLVGRRRRFLDYLNDIDVERYRAIVAELGLRR"
     gene            5934..>7460
                     /locus_tag="PLP95_02190"
     CDS             5934..>7460
                     /locus_tag="PLP95_02190"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HD-GYP domain-containing protein"
                     /protein_id="HPB44641.1"
                     /translation="MAARLLRIAAFLVPLSAGAAASIAASLALPDADTVIGTVARTLL
                     VVAVATVAVRLADKVARRFLPLAALMQLSLVFPDQAPARFRSALRAGSSRRLERAVQE
                     ARTNGLAADPGDAARQVVELIGAIGDHDRRTRGHSERVRLYADLLGEEMKLSTEERQK
                     LQWGALLHDLGKLMVPDEILNKAGKPTDEEWAVLKGHPDAGMDLIAPLRPFLGEWVAA
                     IGGHHEKWNGSGYPKGLVGSQIPRAAAIVAVADSYEVMTATRSYKKPMSISDARAELT
                     RCAGSHFSPEVVRAFLGISLGRLRVVAGPLSALAHLPFLGAIGQVPAVGAVATTPAMA
                     SAFAVPAVAMATTAALVVGAASASADDLVVHAASAEVQSDAATRAAGETAAPSEQNTG
                     GAGSGWWALGEILSGVAPVAAAPVTSIPPQSLAPVESTIPTAPAPTAAPAPTPTVAAT
                     TPAPAPKAAPTTAAPTTAAPAAPVPAAPGDGSGQSGGGRRGDRGSGGWGGHGNGNGNG
                     N"
BASE COUNT         1184 a         2446 c         2525 g         1205 t
ORIGIN      
        1 gagcaggagg tcgagctcca gaaggttctc gacatccccg aggacgacga ggacgatctc
       61 tccgagtacc cccaccgtgc tccggtcatc accgtcatgg gtcacgtcga tcacggcaag
      121 acgaagctgc tcgaccggat ccgcaacgcc aatgtgcagg cgggcgaggc cggcggcatc
      181 acccagcaca tcggcgccta tcaggtcatc aagaacggca agccgatcac cttcctcgac
      241 accccgggcc acgaggcgtt caccgccatg cgtgtacgcg gcgccgaggc gaccgacatc
      301 gtcatcttgg tggtcgccgc cgacgacggc gtcatgcccc agacgatcga ggcgatccag
      361 cacgccaagg cggccgaagt gccgatcgtc gtggcggtca acaagatcga ccgcgaaggg
      421 gccgacccgc aaaaggtcat gagccaggtc gccgagcatg gtctcgttcc cgaggcctgg
      481 ggcggcgaca cggtgttctg tgagatctcg gcgctgcagg aaatcggtat cgacgacctg
      541 ctcgacaacc tgctgctggt ggccgaggtc gaagacctcc gggccgaccc cgaaggccgc
      601 gccaccggcg tggtgctcga gtcgcacctc gacgtcggac gcggtccggt ggccacggtg
      661 ctcgtgcagc ggggcacgct gcgggtgggt gacccactcg tcgccggtcc ggcatgggga
      721 cgcgtccgtg ccctcatcaa cgaccacggc gagcaggtca aagaggccgg cccgtcgacc
      781 ccggtgcgtg tgctcggtct gagcgacgtc gccgactccg gtgacgactt cgtggtcgcc
      841 cccgacgagc gcaaggcgaa gtcggtggcc cagacccgcg aacactggca gcgcgacgcc
      901 cgtcgcggcc gggattcctc ggtcatgtcc ggtggcgcga ggctcgagga catcttcgat
      961 cagatccagc gtggcgagtc ggcgacgctc aacatgatcg tcaaggccga tacctccggt
     1021 tcgctcgagg cgctcatcga gtccctgcgc aagctggaac ggcccgaggt caagatcggc
     1081 ttcgtgttgc gcggcgtggg tggcatcacc gagtccgaca tccagcttgc cgaaacctcc
     1141 gatgccaccg tcgtcggctt caacgtgcgt cccgatcgcc gggtgcgcga gttcgccgag
     1201 gccgagggtg tcgagatccg aacctacgac gtcatctaca agctgctcga ggacgtcgag
     1261 gcggcgatgg tgggcatgtt ggagcccgaa ttcgaggagg tcgtcaccgg cgacgccgag
     1321 gttcgcgaga tcttcaaggt ccccaagatc ggcttcatcg ccggttgtta tgtcctcaac
     1381 ggcaagatca cccgcaactc gcgggtgcgt ttcatccgcg agggcaccgt catctggaag
     1441 ggctccgtgc aatcgctgcg gcgcttcaag gaggacgcac gcgaagtggc cgccggctac
     1501 gagtgcggta tcgggctgtc ggactaccag gacctgcgtc ccggcgacat catcgagacc
     1561 tacgacctcg tcgaaatccc gcggacctga tgcgtcgagc gtgtcgtggt cggggccctg
     1621 ccctgaccac gacacctgcg cgccggtacg gtcgcgtccg cgagaaggaa gcgacgagaa
     1681 ggaagcgacg aggaggaagt gacatggcac gtccaaggca gcgggctcag gcccgccatc
     1741 agttcaaccg gaccgaccgc atcgcggaga cgatccgcga aatcgtcgcg accgaactcg
     1801 aacgaatcga cgacgaacgc gccgacctcg tcacggtgac cggcgtgaag gtcgacaacg
     1861 atctgaacac cgcgaaggtg tactactcgg cgatcgtggc ggaaaccgag ggccgcctcg
     1921 acgacgtggc cgaggcgctc aacgacgccc gatggccgat ccaacaggtc gtcaatcggt
     1981 cggtgcgcgc ccgcaagacc ccacagatcc aattccttcc cgacgacgtg ttggccgcag
     2041 cgttgcggat cgacgacatc ttgaacgacc gcgtccaccc ggccggcgtc gacagcgatg
     2101 ccgatctgcc ggggtccgac ggcgaccccg ccgcggcggg ctgagccggg ccggtcgaat
     2161 atggggcgtc gaggcaaggg cggggagggg accgttcacg gcgtcgcggt cgtcgacaag
     2221 gaggccggct ggacgagcca cgacgtcgtg gccaaggcgc gcgggctgct cggaacccgt
     2281 cgtgtcggtc attcgggcac gctcgatccc gacgcgaccg gggtgttggt cctcggcgtg
     2341 ggccgagcga cgaagctgct gcggttcttg cagctcctcc ccaagagcta tcggtgcgag
     2401 atcatcttcg gcgccgacac ctcgaccctc gacgactccg gcgaggtggt cgccacctac
     2461 gacatggcct tcagtccaca ggaggccgcc gccgcggcgg cgacctttgt cggcgacatc
     2521 atgcaggtgc cgccgatggt gtcggcggtg aaggtcggcg ggcgtcgcct tcacgagctc
     2581 gccgcgaggg gatcgaggtc gaacgcaacc cccggccggt gacgatcatg cgttacgacc
     2641 tcgaggcgac cgcggatccg ctggtgtatc gagccgagat cgagtgcagt tcgggtacct
     2701 acgtgcgcgt cctcgccgag gatctcggcc gtgcgctcgg cggcggcgcc cacatccgac
     2761 ggttgcgacg cacttcggtg ggcccgttca ccctcgacga tgctcgaccg cttgagtcga
     2821 tcgaggcgct gccgatgctc gaggccgtgc gcgggttcga ccgtgtcgtc gtcgacgagg
     2881 acaccgaggc ggcggtacgt gtcggcgcgg tgctcgattc gcagcgcctc ggggtggagc
     2941 cgcacgatcc cggcccgtgg cccgtggtga gcggttcggg ggagttgctg gcgatgtatg
     3001 aaccgttccg cgacggcagg gtgaaaccag cggtggtgct ggtcggatag gttcggcagc
     3061 gatgcacatt gctcgtcagc tcagcgacgt cgtcgatccg cgtgccggca acgtggtcag
     3121 tatcggcttc tacgacggcg tccacctcgg ccaccgtgcg ctcatcacct ccctgctgga
     3181 gatgtcgagc agtcgcggcc tcgccacgac ggtggtgacc ttcgaccagc accccgctcg
     3241 ggtgttgcgc cccgaggtgg cccctcagct gctgtgcgac ctcgaccaga agcttgaact
     3301 gctcggcgaa accggggtcg accagaccct ggtcatccat ttcgacgagg cacaggcggc
     3361 tcaacccgcc gaggacttcg tcacctcggt gctcgtcgac gcgctgcagg cccgcgtcgt
     3421 ggtggtgggg gaggactttc acttcgggca ccggcgcctc ggcaacgtcg ccctgctcac
     3481 cgagatgggc gaaacgcacg gcttcgaggt catcggacat cacctggtcg ggatcgactg
     3541 gaccgacgct cggcccgaac agcaggtgtc atccacggcg attcgtcggg cggtggtcga
     3601 gggacgcatc gaggacgcga acgccatgtt gggtcggcca cacacgatcc gcggcaccgt
     3661 ggtgggcggc gacaagcgcg gtcgcaccat cgggtttccg acggcgaatg tcgtgattca
     3721 tccggagttg ctgcagccgg ccgacgggat ctacgtgggc gaactcgtcc gtgccaacgc
     3781 cgaggtgctg cccgcagcgg tctatcaggg ccggcgaccg acgttctatg aggaccagcc
     3841 gacgtcgatt ctcgaggttc acgccctcga ttgggccgga gacctgtacg gtgaaccggt
     3901 gcaggttcag ttgcacaaac gattgcgcgg cgaccgtaag ttcgacacga tcgaggaact
     3961 gaccgcacaa cttgcgatcg attgccagcg tgccacggag tatctgaggg agagggaccg
     4021 ttcatgaacg cgatggcgat gaactcctcg tcgaccaact cgggcgtcga gccgattccc
     4081 ggcccacatc ctgaaggcgt ggtcgaggac ggcgcaccgg ttgtcgcacc gcggttgagt
     4141 cggcgtcgga tccggctcga cgacggccat gaagtgggca tcagcgtcgc cggtcacggg
     4201 gtgccgttgg tggtcgttca cgggttcagc gccgagggat tcctgtacgc ccagaccctc
     4261 caccgcctgg tgtcgatggg attcaaggtg atcgccatcg ataccgcggg acatggcgga
     4321 acccgagggc ttccctccga cggagccaac ctgcgttcct acggcgagct gctggcccgg
     4381 gtcatcgaca agctcggcat cgaacgctgc attcttgcgg gccactcgat gggtggtcgc
     4441 atggtgaccg acgtcgccgc catgcaaccg gaacgcacgg tcgcggtcat gctcatcgat
     4501 gcgatcgtcg gcgacacgtg ggacaagatg gtgtacgcgt tccgggtgta cccgccgctg
     4561 gtcggcgcga tcggcacggc gttggccgtc gattcgctga cgatcctgcc gctgtggtcc
     4621 gacccgcgtc aggccatcaa gtttttgcgg ctcgccttac ccacgatcat cggcgatgtg
     4681 gtcaggccgt ggcggctgtt gggcccggtg gtgtcgattc tgcgttctcg atcgagccgc
     4741 tattcgctca acgcgctcgg cgatgccggt gttccgttgt atgtgctgca cggcgatcgc
     4801 gacctggcgg tgccgatgcg cacggccagc gacgccgcca agcgggcgaa cgggacgttg
     4861 atcacggtga acggcggcgg ccattcctgg ctgctccgcg acccggagac cctgccgagc
     4921 atcgtgtcgc agttgatgca cggcccgctg agcgaggaga tcaccgcagc catccgctcc
     4981 gccgggatga catccgagga acccaccgac gcggagctcg attcggtgtt ctatcgcccc
     5041 gatgcgctca tccacgagct caccccgacc cgcgaagtca cccacgtggt gggtcggcac
     5101 tggcgtccga cgtttcgctg gaccacctcg gcgccggcct gaccggcgcg tcctgaccga
     5161 cctgtcctga ccgnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5221 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnngtcctga
     5281 ccgacctgtc ctgaccggtg catctgcggg ttggtcggct cgtgtgctag ggtgaccgcc
     5341 gtccaatttg gcgtcttgac gtgccttcgt ggcgcagcac cattggtgca gcgtcgatga
     5401 gtgcgccgcc gagaggccgg gtccgaaacc cacacaggag caacttcgcc atgtcacgtc
     5461 cccccgcaaa tctcccgccc aaggccgaca cgatcgccaa gcacgcacgt ggagagggag
     5521 acaccggttc gcccgaggtg cagatcgcgt tgctcaccga ccggatcaat cacctgaccg
     5581 aacacctcaa gcagcacaag aaggaccacc acagccgtcg tggcctcctg atgctcgtcg
     5641 gtcgccgtcg ccgcttcctc gattacctca acgacatcga cgtcgagcgc taccgcgcca
     5701 tcgtcgccga gttgggtttg cgccgctgac gccgctggtc gccgtgaacg ccccccctgc
     5761 gggctggacg gcgaccaagc ccaaaggcga tggggccgat gggtactttc accggtgggt
     5821 actttcaccg ataggtagaa ataccggtca aggcttgagg gcgtgccgcc gatcactcgg
     5881 cggtgagtcc gtcagttctt ccagccgaga atcgttcgtg gcgtgcacgc ccgatggccg
     5941 cccgcctact tcgtatcgca gcatttctgg tgccgttgtc ggccggagcc gccgcgtcga
     6001 tcgccgcgag cctcgccctg cccgatgccg acaccgtgat cggcaccgtg gcgcgaacgc
     6061 tgttggtggt ggcggtcgcg accgttgcgg ttcgccttgc ggacaaggtc gcccgccggt
     6121 tcctgccgtt ggcagcgctc atgcagttgt cgctggtgtt tccggatcag gcgccggccc
     6181 gctttcgttc cgcgttgcgc gccgggagca gccggcgact tgagcgcgcc gtgcaggagg
     6241 cgcgaaccaa cggcctggct gccgacccgg gcgatgcggc ccgccaggtg gtcgagctca
     6301 tcggggcgat cggcgaccac gaccgccgca cccgtggcca ttcggaacgg gtgcgtctgt
     6361 acgccgatct gctgggcgag gagatgaagc tgtcgaccga ggagcgacaa aagctccagt
     6421 ggggtgccct cctgcacgat ctcggcaagc tgatggtacc cgacgagatc ctcaacaaag
     6481 ccggcaagcc caccgacgag gaatgggccg tgctcaaggg gcaccccgac gccgggatgg
     6541 atctgatcgc gccgcttcgc ccattcctcg gcgaatgggt ggcggcgatc ggcgggcacc
     6601 acgagaaatg gaacgggtcg ggctatccca agggcctcgt cgggtcgcag atcccccggg
     6661 ccgcagcgat cgtcgccgtg gccgactcct atgaggtcat gacggcgact cgttcctaca
     6721 agaagcccat gtcgatcagc gatgcccgcg ccgagctgac ccgctgcgcc ggatcacatt
     6781 tctccccgga agtcgtacgg gcgttcctcg ggatctcgtt ggggcgcctt cgagttgtgg
     6841 cgggtccgct ctcggcgttg gcccatctgc cgttcctggg cgcgatcggc caggttccgg
     6901 cggtcggtgc cgttgcaacg actcccgcca tggcctcggc gttcgccgtt ccggcggtgg
     6961 cgatggcgac cacggcggcg ctcgtggtcg gcgcggcgag cgcctcagcc gacgacctgg
     7021 tggtgcacgc tgcgagtgcg gaggtgcagt cggacgctgc gacccgggcg gcgggcgaaa
     7081 ccgcggcgcc cagcgagcag aacactggcg gtgcgggttc ggggtggtgg gcgctcggag
     7141 agattctctc cggcgttgcg ccggtagcgg ccgcccctgt gacgtcgatc ccgccgcagt
     7201 cgctggctcc ggtggagtcg acgattccga cggctccggc tccgaccgct gctcccgctc
     7261 cgacccctac cgtggctgcg acgactccgg ctccagcgcc gaaggccgcg cccacgaccg
     7321 ccgctccgac gaccgctgcg ccggcagcgc ctgtgccggc ggcgcctgga gacgggtcag
     7381 gtcagtccgg cggcggtcgg cgcggtgatc gcggctcggg aggttggggt ggccatggca
     7441 acggcaacgg caatggcaac
//
LOCUS       DAOOMA010000043         7389 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_11891_length_7389_cov_3.901827, whole genome shotgun sequence.
ACCESSION   DAOOMA010000043 DAOOMA010000000
VERSION     DAOOMA010000043.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 7389)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7389)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7389
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11891_length_7389_cov_3.901827"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..865)
                     /locus_tag="PLP95_02195"
     CDS             complement(<1..865)
                     /locus_tag="PLP95_02195"
                     /EC_number="2.5.1.141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020379243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008495 - protoheme IX farnesyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0048034 - heme O biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme o synthase"
                     /protein_id="HPB44642.1"
                     /translation="MSSTVVSSTPSTSPGIVTKLGGYVALTKPRIIELLLISTVPTMF
                     VAQRGVPGIWLMIATVVGGTLSAGGANAINMYVDRDIDALMSRTKNRPLVTGIIAPRN
                     ALVFAVGLEVVAFFFLWITVNLLSAALAVAACLFYVFVYTLWLKRSSEHNIVIGGAAG
                     AVPVLIGWAAVTNEITLAPIVLFAFMFFWTPPHFWALAIKYKDDYSKAAVPMLPSVAN
                     YDTVTRKILNYTFLVVVFSLLFAVTADLGVIYWTAATVLGALFIWLAVRVRRTESPAI
                     ARMSSNCRFACV"
     gene            985..1977
                     /locus_tag="PLP95_02200"
     CDS             985..1977
                     /locus_tag="PLP95_02200"
                     /inference="COORDINATES: protein motif:HMM:NF014665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_process: GO:0006784 - heme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="COX15/CtaA family protein"
                     /protein_id="HPB44643.1"
                     /translation="MAAERFRPTPRGYERIVQISVAALSSIIVTGAAVRLTGSGLGCR
                     DWPTCEDGELVASLSFHPMIEFVNRLITGLVSIAVVLAVLGSLRRRPRRSDLTRWSWV
                     LVIGVVAQILIGAAVTLSELRYSVVAVHFLVSMMLVWAAMVLWKRSTDPDHETEPGKA
                     VAAIRRTWSFESKLLVTLGALVLFGGAMVTSAGAHTGAKVGHDGTEILIERLPIELRP
                     VVQLHSATVWLLCLMTVWMVLRSARRGVPSREAKDLLIAVVAQGALGYWQYFAGVPAL
                     LVGFHVAGATLVWILALRLAFAEAAVTPRANPEADSRTPYPSTPALAGSKAEAS"
     gene            1974..2279
                     /gene="clpS"
                     /locus_tag="PLP95_02205"
     CDS             1974..2279
                     /gene="clpS"
                     /locus_tag="PLP95_02205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012229018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adapter ClpS"
                     /protein_id="HPB44644.1"
                     /translation="MTDDSPLEPSGPVGVLERDEASEVDSPWVVLVWDDPINLMEYVT
                     FVFQKVFGYSKTKATRLMMQVHNEGKAVVSSGDRASAEVDVLRLHEHGLWATMQQDT"
     gene            2279..3193
                     /locus_tag="PLP95_02210"
     CDS             2279..3193
                     /locus_tag="PLP95_02210"
                     /inference="COORDINATES: protein motif:HMM:NF020989.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2017 family protein"
                     /protein_id="HPB44645.1"
                     /translation="MSTSDGEDLFDDVPRDGEWAPDSPWLSQVDDDRFELHLPVDVHE
                     VLTAGLAHLAGDVAAGVEIAENDRGRRRILGREELMAIVDMCADKVRYGLEHQDFTEG
                     LLEEFAGAVSTVATVASADLEHRRLSGQDVSPRARWVDALDHLTTNCTTMLFTRFRPR
                     RDGTFALRWHPADRKLIEDAVEELDRLLESDDPAIERLFPAPYGDDEERNAAWSILAR
                     SELIDRRRASLETVRRLMDTKRATAEELSMLMRTVNDSRLVFGTQLGITEPGGVATGT
                     DEQRRLHRVYELLGGIVYDAVTALRSTL"
     gene            3255..5276
                     /locus_tag="PLP95_02215"
     CDS             3255..5276
                     /locus_tag="PLP95_02215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012228750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter Kup"
                     /protein_id="HPB44646.1"
                     /translation="MTDQSPSDGVADADSSVGQHGASEPEPAESATDPELAESASTSE
                     AADSGHGVPKPIAALTIGALGVVFGDIGTSPLYSLKEAFHEHHDRTLEISDASVLGLL
                     SLVFWSLVIVISIKYIFYVMRADNDGEGGILALTALVRPDGTKAKANAGKLVLFGLFG
                     AALLYGDGMITPAISVLSALEGTETVTDSMTRYVVPIAIVILVALFWIQRRGTATVGR
                     IFGPVMVVWFLTLGVLGLIHIFDDPSVFRAIAPWYAVDFFIQNGMLGLFCLGSVVLVV
                     TGGEALYADMGHFGRRPIKLAWFAMVLPGLMLNYFGQGAMLLSHPGYIDNPFYRMAPS
                     WSVWPLVVLSTAATVIASQALISGAFSLTRQAVQLGYLPRVRISHTSDSEEGQIYVPA
                     INWLLCAACVALVLGFRTSNNLAAAYGLAVTGTMVITTVLFYRVARDRFGWPKAKAMW
                     ILGGLLIVDLGFLSANIPKIPDGGWVPLAIGAAFLIVFTTWYTGRRITARRLAAEAKP
                     LESFIELLDERDIMRNPGLGVYMGSNPDIVPQALSSHLRHARVLPSTVVVLAIKFENR
                     PYVTGQDRIAYTDLGHGVHRIVHHSGFSDTIDIPALLAEEAPLYTPLSFDSATFILGR
                     ETLRVTGRPGMAIWREHLFVFMLRNATPADAFFKLPPDRTVELGVQVEI"
     gene            complement(5328..5400)
                     /locus_tag="PLP95_02220"
     tRNA            complement(5328..5400)
                     /locus_tag="PLP95_02220"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5364..5366),aa:Glu,seq:ttc)
     gene            5568..5642
                     /locus_tag="PLP95_02225"
     tRNA            5568..5642
                     /locus_tag="PLP95_02225"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5603..5605,aa:Asp,seq:gtc)
     gene            5704..5776
                     /locus_tag="PLP95_02230"
     tRNA            5704..5776
                     /locus_tag="PLP95_02230"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5737..5739,aa:Phe,seq:gaa)
     gene            complement(5831..7231)
                     /locus_tag="PLP95_02235"
     CDS             complement(5831..7231)
                     /locus_tag="PLP95_02235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015799327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent malic enzyme"
                     /protein_id="HPB44647.1"
                     /translation="MDHSTTAAYSVRVQVRMSNEPGMLGRLTLAIGEAGANILGMGGF
                     EVKSETVVDDIIINCSSVAHQEAVRSVIEGLEGIEVLDWQDRTFAMHEGGKITSIAKS
                     PLVDADDLSMAYTPGVARVCMEIAKNEARSHDLTIRKNTVAIVSDGTAVLGLGDIGPY
                     GAMPVMEGKALLFKHFADVDAFPICIKASTADEIVDLVMRLEPTFGGINLEDIKAPEA
                     FEVEERLVEALDIPVFHDDQHGTAIVTLAALQNALKIVDKRIDEVNVVVAGVGAAGVA
                     VTKILMNAGVRHVIGVDRSGCVFSGREGLNSSKQWFAENTNRGEVQGSISNAIKGADV
                     FIGLSGPDLITVDDVRSMASDPIVFAMANPDPEIRPELIHDIAAVIATGRSDFPNQIN
                     NVLAFPGIFRGAFDAGARKITENMKVAAANAIAEVVGDDLTAEYIIPSVFDDRVAPAV
                     AEASRRAAIADGVCRS"
BASE COUNT         1291 a         2404 c         2330 g         1364 t
ORIGIN      
        1 tcacgcaggc gaagcggcag ttggaagaca tcctggcgat cgccggcgac tcggtgcgtc
       61 ggactcgtac cgccagccag atgaacagcg ctccgagcac cgtcgctgca gtccagtaga
      121 tgacgccgag atcggcggtc accgcgaaga gcaggctgaa caccacgacc aagaatgtgt
      181 aattcaggat ctttcgcgtc acggtgtcgt agttcgccac cgacggcaac atcggtaccg
      241 ctgcctttga gtaatcgtcc ttgtacttga tcgcaagggc ccagaaatgc ggtggggtcc
      301 agaagaacat gaaggcgaac aacacgatcg gcgccaacgt gatttcgttg gtgaccgccg
      361 cccagccgat taagacaggc acagcgccgg cggccccgcc gatcacgatg ttgtgttccg
      421 aggaccgctt caaccaaagg gtgtagacga acacatagaa caggcaggcg gccacggcca
      481 gcgctgccga cagcaggttg accgtgatcc agaggaagaa gaaggccacc acctcaaggc
      541 ccaccgcgaa cacgagcgca ttgcgcggcg cgatgattcc ggtcactaag gggcggttct
      601 tcgtgcggct catcaacgca tcgatgtcgc ggtcaacgta catgttgatg gcgttcgcac
      661 cgccagccga caacgtccca ccaacgacgg tcgcgatcat cagccagatg ccgggaaccc
      721 cacgctgagc gacgaacatc gtcggtacgg tgctgatgag caggagttcg atgatgcgcg
      781 gcttcgtgag cgcgacgtag ccgccgagct tcgtgacgat tcccggcgac gtggacgggg
      841 tggatgacac gaccgtggaa gacaccacgg cctaagttac gcccagccgg cggcggtggg
      901 ccaatgcagc cgttcaggcg agtgggcacg cccgatggac aagccgatgt gaacccgctt
      961 cgccctaacg cctagcgtga ccccgtggcc gcagaacgat tccgtccgac gccgcggggc
     1021 tacgaacgca tcgtgcagat ctccgtcgcg gcgctttcca gcatcatcgt caccggcgcg
     1081 gcggtgcgcc tgacggggtc cgggctcgga tgccgcgatt ggccgacctg tgaggacggt
     1141 gagctcgtag cgtccctgtc gtttcacccg atgatcgagt tcgtcaaccg cctgatcacc
     1201 ggcctcgtat cgatcgcggt ggtgctcgcg gtgctcggct cgctgcgtcg acggccgcga
     1261 cgctccgatc tcacccgctg gtcgtgggtc ctcgtcatcg gggtggtcgc acagattctg
     1321 atcggcgcgg cggtcacgct gtcggagctg cgctactcgg tcgtcgcggt gcatttcctc
     1381 gtctccatga tgctggtgtg ggccgcgatg gtgctctgga aacgctcgac cgatcccgat
     1441 catgagaccg aacccggcaa ggccgtcgcc gcaatccggc gaacgtggtc cttcgaatcg
     1501 aagctgctcg tgaccttggg ggcgttggtc ctgttcggcg gggcgatggt gacgagcgcg
     1561 ggggcacaca ccggagcgaa ggtcggccac gacgggaccg agatcctgat cgaacgcctc
     1621 cccatcgagt tgcgtcccgt cgtccaactg cactcagcga ccgtgtggct gttgtgtctg
     1681 atgaccgtct ggatggtcct acggtcggcg agacgaggag tgccttctcg cgaggccaag
     1741 gacttgctca tcgcggttgt cgcccaaggc gctctcggct actggcagta cttcgccgga
     1801 gtgcccgccc tcctcgtcgg gttccacgtg gcgggcgcga cactggtctg gatccttgcg
     1861 ctgcgtctcg cctttgccga ggcggcggtc acgcctcgtg cgaaccctga ggccgacagc
     1921 cgcacgccgt acccgtccac cccggcactg gccggctcga aggcggaggc ctcatgaccg
     1981 acgactcccc gttggagccg agcggacccg tcggcgttct tgaacgcgac gaggcgagcg
     2041 aggtcgattc cccctgggtc gtgctggtct gggacgaccc gatcaacctc atggaatacg
     2101 tgaccttcgt tttccagaag gtcttcggtt actccaagac caaagccacc cggttgatga
     2161 tgcaggtcca caacgaggga aaggcggtgg tcagttcggg agatcgtgcc agcgccgagg
     2221 tcgacgtgct gcggctccac gagcacggcc tgtgggcgac catgcaacag gacacctgat
     2281 gtcgacgtcc gacggggaag acctcttcga tgacgtcccg cgcgacggcg aatgggcccc
     2341 tgattcccct tggctcagcc aggtcgacga cgaccgtttc gagttgcacc tcccagtcga
     2401 cgtgcatgaa gtcctgacag cggggctggc ccacctggcc ggcgatgtcg ccgccggtgt
     2461 cgagatcgcc gaaaacgacc gcgggcgacg ccggatactg ggccgcgagg agttgatggc
     2521 catcgtcgac atgtgtgccg acaaggtgcg ctacggcctc gaacaccagg acttcaccga
     2581 gggactgctc gaggagttcg ccggcgccgt ctccaccgtg gccactgtcg cgtctgcaga
     2641 cctcgaacat cgccggctca gcgggcaaga cgtttccccg cgcgctcgtt gggtggacgc
     2701 gctcgaccac ctcacaacaa actgcacgac gatgctgttc acccgattcc gcccacggcg
     2761 cgacgggacc ttcgcgctcc gctggcaccc cgccgatcgc aagctcatcg aggacgcagt
     2821 cgaggaactg gaccggctgc tggaatcgga tgacccggct atcgaacggc tatttcctgc
     2881 cccatacggg gatgacgagg aacgcaatgc cgcgtggagc attcttgccc gctcggagtt
     2941 gatcgatcgg cggcgagcgt cactcgagac cgttcgtcgc ctgatggaca ccaagcgagc
     3001 gacggccgag gagctctcga tgttgatgag gacggtgaac gactcccgcc tggtcttcgg
     3061 cacccaactt gggatcaccg aacccggcgg agtcgccacc gggaccgacg aacaacggcg
     3121 gcttcaccgg gtctatgagt tgctgggcgg aatcgtctat gacgccgtca ccgcactccg
     3181 ctcgacgctg tagcaacggt tcaactcgcc aactacgccc gggtcgtgcc gagtgcgtat
     3241 actgctgaaa acctatgacc gatcagtcgc cttcagatgg tgtcgcggac gccgattcct
     3301 cggtgggcca gcacggtgca tccgagcccg agcccgccga atcagcaacc gatcccgaac
     3361 tcgccgagtc cgctagcacc tccgaggccg ccgacagcgg tcacggggtt ccgaagccca
     3421 tcgccgcgct gaccatcggt gcgctcggtg tcgtcttcgg cgacatcgga accagcccgc
     3481 tgtactcgct gaaggaagcg ttccacgaac atcacgatcg gacgctcgaa atcagcgacg
     3541 cgagcgttct cgggctgtta tcgctggtgt tctggtcgct ggtgatcgtg atctcgatca
     3601 agtacatctt ctacgtgatg cgcgccgaca acgacggcga gggtggcatc ttggcgttga
     3661 cggcgctcgt gcgtccggat gggacgaagg ccaaagccaa cgccggcaag ttggtgctgt
     3721 tcggcctgtt cggtgccgct ctcctgtacg gagacggcat gatcaccccc gccatctccg
     3781 tactttccgc gctcgaaggt acggagacgg tgacggactc gatgacgcgg tacgtcgtgc
     3841 cgatcgcgat cgtgatcctc gtggcgctgt tttggattca acgacggggc accgccacgg
     3901 tcggccggat attcgggccg gtgatggtgg tgtggttcct cacgctcggg gtgctcgggc
     3961 tgatccacat cttcgatgat ccgtcggtgt ttcgagctat cgcaccgtgg tacgcagtcg
     4021 acttcttcat tcagaacggg atgctcgggc tgttctgcct gggatcggtc gtactggtcg
     4081 tcaccggtgg cgaggccctc tacgccgaca tgggccactt cggtcggcgt ccgatcaagt
     4141 tggcctggtt cgccatggtg ctccccgggc tcatgttgaa ctacttcgga cagggggcga
     4201 tgctgctcag tcatcctggc tacatcgaca atcccttcta tcgcatggct ccgtcctggt
     4261 cggtgtggcc gctggtggtg ttgtcgaccg ccgcgacggt gatcgcatcc caagccctca
     4321 tctccggggc gttctccctc acgcgccaag cggtacaact cggctatctg ccccgcgtgc
     4381 ggatctccca tacctccgac agcgaggagg gccaaatcta cgtgccggcg atcaactggt
     4441 tgctgtgtgc cgcatgtgtc gccttggtgc tgggatttcg cacctcgaac aatctcgccg
     4501 ccgcgtacgg cctcgcggtc accggaacca tggtgatcac gacggtgttg ttctaccgcg
     4561 tcgcccgcga ccggttcgga tggccgaagg ccaaggcgat gtggatcctc ggcgggctcc
     4621 tcatcgtcga tctgggcttt ctgtcggcga acattccgaa gattcccgac gggggttggg
     4681 ttccgctcgc catcggggca gccttcctga tcgtcttcac gacctggtac accgggcgtc
     4741 gcatcaccgc acggcgtctc gccgctgaag ccaaaccgct cgagtcgttc atcgaactgc
     4801 tcgacgagcg cgacatcatg cgcaatcccg ggctcggggt ctatatggga tcgaatcccg
     4861 acatcgttcc ccaagccctt tcctcacacc tgcgacatgc gcgggtgctc ccgtcgaccg
     4921 tggtcgtcct cgccatcaag ttcgagaacc ggccgtacgt caccggacag gatcggatcg
     4981 cctacaccga cctcgggcac ggagtgcacc gcatcgtgca ccacagcggg ttctccgaca
     5041 cgatcgacat accggcgctg ctcgctgagg aagcacccct gtacacgccg ctctcgttcg
     5101 actcggcgac cttcatcctc ggccgtgaga ccctgcgcgt gaccgggcga ccggggatgg
     5161 cgatctggcg tgaacacctc ttcgtgttca tgctccgcaa cgcgaccccg gccgacgcct
     5221 tcttcaagtt gccgcccgac cgcaccgtcg agctcggagt ccaggtcgaa atctgacgtc
     5281 aggcaagtcg tctcggcggc ccgcactcgg cgcgtacccc aacggtgtac ccccaacggg
     5341 attcgaaccc gtgctgccgc cgtgaaaggg cggtgtccta ggccgctaga cgatgggggc
     5401 ctgcccgtta gggcgcgatg cagcttagca gccccacgtc gtctcagtcc taatcggtcg
     5461 gctcgccatc agacgtcacc gtgatcgacc cacacgaatt ttcttcgacc gagttggcat
     5521 cgcccgaccg cccatctgta aagtagccgt cggccaatcg gccagaaggt cccgtggtgc
     5581 agtctggagt gcacgccgcc ctgtcaaggc ggaggtcgcg agttcaaatc tcgtcgggac
     5641 cgcaaccccc tcgcctcggc gggggtgtcg tcgtcatcgg gcgttcgtcc ctcgtcgaca
     5701 ctcggtcagg tagctcagtc ggcagagcga ccgcctgaaa agcggtaggt ccggggatcg
     5761 acgccccgcc tgaccacctc caatgagaag ggccccgcca cggcggggcc cttgtcgctt
     5821 ctccgatcag ctacgaccga cacacaccat cggcgatcgc ggctcgacgc gaggcctcgg
     5881 caaccgctgg tgcaacccga tcatcgaaca ccgaggggat gatgtactcg gcggtgagat
     5941 cgtcgcccac gacctcggcg atggcattgg ctgcagcgac cttcatgttc tcggtgatct
     6001 tgcgggcacc ggcgtcgaag gccccgcgga agattccagg aaaggccaac acgttgttga
     6061 tctggttcgg gaagtcggaa cgcccggtcg cgatgaccgc cgcgatgtcg tggatcagtt
     6121 cgggtcggat ctcagggtcg gggttcgcca tagcgaacac gatcggatcc gaggccatcg
     6181 accgcacgtc gtcgaccgtg atgagatcgg gtccggacag cccgatgaac acgtcggcgc
     6241 ccttgatggc attcgagatc gagccctgca cctcgccacg gttggtgttt tctgcaaacc
     6301 attgctttga cgagttgagc ccctcgcgtc ccgagaagac gcacccggag cggtcgacac
     6361 cgatgacgtg gcgaacgccg gcattcatca gaatcttcgt gaccgcgacg cctgccgcac
     6421 ccacaccggc gacgacgacg ttcacctcat cgatacgctt gtcgacgatc ttgagggcat
     6481 tttgcagcgc cgccaacgtc acgatcgcgg tgccgtgctg gtcgtcgtga aagaccggga
     6541 tgtcgagcgc ctcgacgaga cgctcttcaa cctcgaaggc ttcgggggcc ttgatgtcct
     6601 cgaggttgat gcccccgaag gtcggctcga ggcgcatgac aaggtccacg atctcatccg
     6661 cggtgctggc cttgatgcag atcggaaagg catcgacatc ggcgaagtgc ttgaacaaca
     6721 atgccttgcc ctccatcacc ggcatggcgc cgtaggggcc gatatctccg aggccgagca
     6781 ccgctgtgcc atccgagacg atggcgacgg tgttcttgcg gatggtcaga tcgtggctgc
     6841 gagcctcgtt cttcgcgatc tccatgcaga cccgcgcgac cccgggggtg taggccatcg
     6901 acaggtcgtc cgcgtcgacc aggggcgact tcgcgatcga ggtgatcttg cccccctcgt
     6961 gcatggcgaa ggttcgatct tgccagtcga gcacttcgat gccctcaagg ccctcgatga
     7021 cgctgcggac cgcctcctga tgcgcgacgc tcgaacagtt gatgatgatg tcgtcgacga
     7081 cggtctcgct cttcacctcg aatccgccca tccccaagat gttggcgccg gcctcgccga
     7141 tagcgagcgt caggcgtccg agcatgccgg gttcgttcga catgcgaact tgaactcgga
     7201 ccgaataggc ggcggtcgtc gaatgatcca tgaaccggca gtctacgaaa cggacgtttg
     7261 cggaacgaac ctgagatggg gtttcgttga tcgagttctc tgcggatcga gcgccgtgcg
     7321 aaccgagcgc ggccgctcag ccctcggcag tgtcgcggtc cagcgcgaac ccgagatcct
     7381 ccgccgtgg
//
LOCUS       DAOOMA010000044         7372 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_11925_length_7372_cov_3.808528, whole genome shotgun sequence.
ACCESSION   DAOOMA010000044 DAOOMA010000000
VERSION     DAOOMA010000044.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 7372)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7372)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7372
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11925_length_7372_cov_3.808528"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..522)
                     /locus_tag="PLP95_02240"
     CDS             complement(<1..522)
                     /locus_tag="PLP95_02240"
                     /inference="COORDINATES: protein motif:HMM:NF025690.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4388 domain-containing protein"
                     /protein_id="HPB44648.1"
                     /translation="MALQGNLESFPLVDVLGMLSSSQKSGRLVVNADRCGAALWLELG
                     SIVGATTSAPGQSAQRSEPASVLFDLLRCREGAFYFEPDLRCPEPCAPTDTSTAISEA
                     QAALEEWTMIVATVPSLTSTLTLQTSPPGTEVDFDERRWLALCAVVDVSIRDGFANVC
                     ALVEHLGLGELDAL"
     gene            complement(671..1513)
                     /gene="aroE"
                     /locus_tag="PLP95_02245"
     CDS             complement(671..1513)
                     /gene="aroE"
                     /locus_tag="PLP95_02245"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HPB44649.1"
                     /translation="MSEPSLAAGPTGRSAFVIGDPIEHSKSPDLHNAAFAAAGLPHVY
                     GRIRVGVDEVGEVIAEMRRQRWLGLSVTMPDKEAVIAHLDRLTPSAELLGAVNCVMWD
                     GDDLVGDNTDGAGLVWAIRDHLGVDVRTAIGVVGAGGAARAAMVALAEAGANSITVVN
                     RTRSRAEAAVATTNAAVAGVARVGEAADLTSCGIVLNATPQGMGDAGVASLPAYVPTE
                     GQLAYDLVYHPRVTAWMAAAEARGARTANGLSMLIGQAAVQFERWTGVKASTEAMLRA
                     VERD"
     gene            complement(1510..2667)
                     /gene="mltG"
                     /locus_tag="PLP95_02250"
     CDS             complement(1510..2667)
                     /gene="mltG"
                     /locus_tag="PLP95_02250"
                     /inference="COORDINATES: protein motif:HMM:TIGR00247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HPB44650.1"
                     /translation="MSYDDHDGYDNDYYYDDGYADANYDDGYDYEPYRPMARRANPIG
                     LTIVAVIAVIGVVAAGTLVWTARQINPSGEAGPAIAEVVIAKGATLSDVADQLESEEI
                     ISSARVMGWYSKFADVKPVQAGRYINFEKNMAMGDAIDVLNGGPVAEQSRVVTIIPGM
                     WLVDALGAINKVFPEISVDEMSLVLASGQVTSKYHTDPTQNWEGYLLPETIDFKSDAT
                     AQEILQRFVSEFDKVLDAAGYGNAEARTGYSAAELVTMASIIEREKGDVEGEANRVAR
                     VIFNRLDKPMRLQIDATVLYGVQRRGNEGGLTKTELETDTPYNSYTRDGLPPTPIALS
                     SKTSLEAAISPAAGDWIYYVLKSVGPREHLFTASYDEFLAGKKVCQQNGWC"
     gene            complement(2664..3107)
                     /gene="ruvX"
                     /locus_tag="PLP95_02255"
     CDS             complement(2664..3107)
                     /gene="ruvX"
                     /locus_tag="PLP95_02255"
                     /inference="COORDINATES: protein motif:HMM:NF015607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HPB44651.1"
                     /translation="MRAIGLDLGSKRIGVALSTSDGVLATPYEVVQRSGDRQRDHRKI
                     RDLALEAEAEVIVVGLPLHLDGRLSESANKALAECDELRATTGLPVETYDERLTTVTA
                     ARSLQELNLSPAARKRAIDQVAAAVMLQAWLDHRRAAADAPEQDP"
     gene            complement(3121..5706)
                     /gene="alaS"
                     /locus_tag="PLP95_02260"
     CDS             complement(3121..5706)
                     /gene="alaS"
                     /locus_tag="PLP95_02260"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HPB44652.1"
                     /translation="MRANDLRLAFTNYFAEKGHTVVPSAGLIPHHPSAPMFNNSGMMQ
                     FVPYFLGEEPVPFNPPRAVSVQKCVRAGGKHNDLDAIGRSPRHLSFFEMLGNFSFGDY
                     FKAEAIAMAWEFVTSVLGVDGDRIWVTVHTSDDDAEDIWADSIGVPRARIQRLDADNF
                     WEMGETGPCGPSSEIFYDFGPDLGPDGGPANPAAEHRFVEIWNLVFMQYFRGADGVLA
                     NLPTPNVDTGAGMERILALLGGSHSLYSADVLADLVGEAERVTGHRIGDGEITDVALR
                     LMADHTRTMTFLVSDGVIPSNEDRGYVLRRVIRRAIRFAYLTGVDRPVLAGLVSRCIE
                     VMGDAYPELRTNSDAVLTTVEREESKFRQTLKTGSALLDSELDKLPTGGRLDGAVAFK
                     LHDTFGFPLEVTQEFVEIAGYELDQHGFDEAMADQRDRAKASAKKGKVATDSESSAFA
                     QLREGGETVFVGREVEQCEANVLGVVGNAIVLDRTPFYAESGGQIGDTGLISWGSHQV
                     HISDTTYGAPGLVRHVVAPDDLDRLAALEVGERVTASIDSPRRAAIRRHHTATHILHW
                     ALREVLGDHVKQQGSWVGPDRLRFDFSHFEALSREQIRDIEDLANEQILSNAVVTHTE
                     MAKQDALELGAIAFFGDKYGDRVRVLQAGPRSIEFCGGTHVSSLGEIGPVKITSETSI
                     GSNIRRLEAVAGTGLIDRLRSVEDRIVEASDALGVPVEDLIDAVRKRIEEAKSSKALI
                     AELRRQLAASAAGDLLDDAVDNKLVAEVASGTRDEVRDLAVSLRDKGLDAVVLISSPG
                     GKGVAVASATKVGGDLNAGELIGEAMKLLGGGGGKGADLATAGGRNVEAIGDAIANVR
                     AKLGI"
     gene            complement(5719..6180)
                     /locus_tag="PLP95_02265"
     CDS             complement(5719..6180)
                     /locus_tag="PLP95_02265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB44653.1"
                     /translation="MFKRVFWLGVGAATGSAATVWGQRKVKQQVEAIQSKATPSHVVD
                     VAKTKATDLKDRVVTAVNEGIAEGRQTEADLRAGLPRSFAGSAAATTASAADNAAALA
                     NSVSNFAASAADRFDAKAADAKAADVAGDTEATRGSARRSRNPDRRRKQAR"
     gene            complement(6180..6542)
                     /locus_tag="PLP95_02270"
     CDS             complement(6180..6542)
                     /locus_tag="PLP95_02270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB44654.1"
                     /translation="MSATDIVLIVTTVVSLGAIAALAITVGQLRLVVRSLRKELDAFT
                     EVAGPSAERLEAAADTAARQVARLENLIDVAGSVTETMDGATASAVRVLSNPVIKTAA
                     VARGTRKAARRLTKSEES"
     gene            complement(6682..>7372)
                     /locus_tag="PLP95_02275"
     CDS             complement(6682..>7372)
                     /locus_tag="PLP95_02275"
                     /inference="COORDINATES: protein motif:HMM:NF023428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB44655.1"
                     /translation="TLIGATTESPWATVNRPLLSRCTVLKLEPLGDADLRALLDRGVE
                     ELAKRRPISVAEDAADHLIGSVEGDGRRLLTGLEAAADLSRPGSGGEITFDLLTEALG
                     AGIAHYQAGDHYDAASAFIKHMRSGNAEQASAWLNHMLDRGEDPRFLARRMVIFASED
                     IGPADRSALIIATGAAHASEFIGMPEVRHNLTHAAIVLSRAAKSREVCDFIAAASTTK
                     LEPPPESAYMH"
BASE COUNT         1176 a         2555 c         2378 g         1263 t
ORIGIN      
        1 caacgcatcg agttcaccca ggccgaggtg ctcgacgagc gcacaaacgt tggcgaaccc
       61 gtcgcggatc gacacgtcga ccaccgcaca caacgccaac caccgccgct catcgaagtc
      121 gacctccgtg cccggaggcg aggtttgcag cgtcagtgtg gacgtcagcg atggcaccgt
      181 ggcgacgatc atcgtccact cctccagcgc tgcttgcgcc tcggagatcg ccgtcgacgt
      241 gtcggtgggg gcgcacggtt caggacagcg caggtccggc tcgaagtaga acgcgccctc
      301 cctgcaccgc aacaggtcga acaacacgct tgcgggctcg ctgcgctgcg ccgactgtcc
      361 cggcgccgag gtcgtcgcgc ccacgatcga ccccaactcc agccacagcg cggcaccgca
      421 gcggtcggcg ttgaccacga gccgaccgct cttttgagag gaggacaaca tccccaacac
      481 gtcaacgagc ggaaacgact cgaggttccc ttgcagtgcc atcgttcctc cagcgtcgaa
      541 tggtcccggg tctctatcgg cacgtcgacc cgatccatga agcccaaaag cccggcttta
      601 tcgcagatcc gtcgattcgg tggcgctcac ccgcccacgg ccgccgtcac tcgcctgccc
      661 ccagcagccg tcagtcgcgc tcgaccgccc tcaacatcgc ctccgtcgac gccttcaccc
      721 cggtccagcg ttcgaactga accgcggcct gcccaatcag catcgacaaa ccgttcgccg
      781 tccgtgcgcc gcgtgcctcc gcggcggcca tccacgcggt gaccctcggg tgatacacga
      841 ggtcataggc cagttgccct tcggtcggaa cataggccgg cagcgacgcc acgccggcat
      901 ctcccatgcc ctgcggggtt gcgttgagca cgatcccaca cgaggtcagg tccgcagcct
      961 ccccgacccg agcgacaccc gccacggccg cgttcgtcgt cgccaccgcg gcctcggcgc
     1021 gacttcgggt ccggttgacg acggtgatcg agttcgctcc ggcctcggcg agcgccacca
     1081 tcgcagcacg ggccgcgcca ccggcgccga ccacgccgat ggcggtgcgc acgtccacgc
     1141 ccaggtggtc gcgaatcgcc cagaccaacc cggcgccgtc ggtgttgtct cccaccaggt
     1201 cgtcgccgtc ccacatcaca cagttgaccg ctcccagcaa ttccgccgac ggcgtgagcc
     1261 gatcgaggtg ggcgatcacc gcctccttgt cgggcatcgt gaccgacagg ccgagccacc
     1321 gttgacgacg catctcggcg atcacctcgc cgacctcgtc gacgccgacg cgaatgcggc
     1381 catacacatg aggcaacccg gcggcggcga acgcggcgtt gtgcaggtca ggggatttcg
     1441 aatgctcgat cgggtcgccg atcacgaacg ccgatcggcc agtcggcccc gctgcgaggc
     1501 tcggttcgct caacaccagc cgttttgctg gcagaccttc ttgccggcca gaaactcgtc
     1561 gtagctcgcg gtgaacaggt gctcgcgcgg cccgacgctc ttgagcacgt aatagatcca
     1621 gtctccggcg gccggactga tggccgcttc gagggaggtc ttcgacgaca aggcgatcgg
     1681 ggtcgggggc agcccgtcgc gggtgtagct gttgtacggc gtgtcggtct cgagttccgt
     1741 cttggtgaga ccgccctcgt tgccccggcg ttgcacgccg tagagcaccg tcgcatcgat
     1801 ctgcaggcgc atcggcttgt cgagacgatt gaagatgacc cgagccacgc gattcgcctc
     1861 gccctcgacg tcgccctttt cacgctcgat gatcgaggcc atcgtgacga gttccgctgc
     1921 gctgtagccg gttcgggcct cggcgttgcc atatccggcg gcgtcgagca ccttgtcgaa
     1981 ctcgctgacg aaacgctgca ggatctcctg ggccgtcgca tcggacttga aatcgatggt
     2041 ctccggcaac aggtacccct cccagttctg ggtggggtcg gtgtggtatt tcgaggtcac
     2101 ctgaccggac gcgagcacca ggctcatctc atcgacggag atctcgggga acaccttgtt
     2161 gatcgccccg agagcgtcga cgagccacat gccgggaatg atggtgacga cccggctctg
     2221 ttcggcgacc gggcccccgt tgagcacgtc gatggcatca cccatcgcca tgttcttctc
     2281 gaaattgatg tacctgccgg cctgcaccgg cttcacatcc gcgaacttcg aataccagcc
     2341 catgacccga gcgctggaga tgatctcctc ggactcgagc tggtcggcga cgtcgctcaa
     2401 cgtggcgccc ttcgcgatga cgacctcggc gatcgccggc cccgcctcac cggacgggtt
     2461 gatctgtcgc gcggtccaca ccaatgtgcc ggcggcgacc accccgatca ccgcgatcac
     2521 cgcgacgatg gtcaacccga tcgggttcgc ccgacgggcc atcggccgat acggctcgta
     2581 gtcgtacccg tcgtcgtagt tggcgtctgc gtacccgtcg tcgtagtagt agtcgttgtc
     2641 gtaaccgtcg tggtcgtcgt agctcacgga tcctgctcgg gtgcgtcggc ggcggcccgg
     2701 cggtgatcga gccacgcctg cagcatcacc gcggcggcga cctgatcgat ggcccgcttt
     2761 cgggccgccg gactcaagtt caattcctgc agcgaccgcg ccgcggtcac cgtagtcaga
     2821 cgctcatcgt aggtctccac gggtagcccg gtggtggcac gcagttcgtc gcactcagcc
     2881 agcgccttgt tggcgctctc gctcaggcga ccgtcgaggt gcagcggcag cccgaccacg
     2941 atgacctccg cctccgcctc gagcgcaaga tcacggatct ttcggtgatc gcgctggcga
     3001 tccccgctgc gctgcacgac ctcatagggg gtggcgagca cgccgtcgct ggtgctgagt
     3061 gcgaccccga tccgtttcga acccaggtcc agcccgatcg ctcgcatcgc gatcgacgcc
     3121 tcagatgccg agcttggcgc gcacgttcgc gatcgcgtcg ccgatcgcct cgacattgcg
     3181 tccgcccgcc gtggcgaggt ccgcgccctt gccgccgcca ccgccgagca gtttcatggc
     3241 ctcgccgatc agctcgcccg cgttcaggtc gcctccgacc ttggtggccg acgcgacggc
     3301 gaccccttta ccgccggggg aactgatgag caccaccgcg tcgagcccct tgtcgcgcaa
     3361 cgacacagcg aggtcacgaa cctcgtcgcg ggtccccgag gcgacttcgg ccaccagctt
     3421 gttgtcgacc gcatcgtcga ggagatcgcc ggccgccgat gctgcgagtt ggcggcgaag
     3481 ctcggcgatc agggccttcg acgacttggc ttcctcgatg cgcttgcgaa ccgcatcgat
     3541 cagatcctcc acgggcacgc ccaaggcatc ggatgcctcg acgatccggt cttcgacgga
     3601 ccgcagccga tcgatcagcc ccgtccccgc gaccgcctcg aggcgacgga tgttcgaacc
     3661 gatcgaggtt tcgctcgtga tcttcaccgg cccgatctcg ccgagcgagc tcacgtgggt
     3721 acccccgcag aactcgatcg agcgaggccc ggcctgcaac acccgaacgc ggtcgccgta
     3781 cttgtcgccg aagaacgcga tcgcaccgag ttccaacgcg tcctgcttgg ccatctcggt
     3841 gtgggtcacc acggcgttgg acaggatctg ttcgttcgcg aggtcttcga tgtcgcggat
     3901 ctgttcacgt gacagggctt cgaaatggct gaagtcgaaa cgcagccggt cggggccgac
     3961 ccacgaaccc tgctgtttga cgtgatcgcc gagcacctcg cgaagcgccc agtgcaagat
     4021 gtgagtggcg gtgtggtggc gacgaatcgc ggctcggcgc ggcgagtcga tcgacgcggt
     4081 cacccgctcg ccgacctcga gcgcggccag tcggtcgagg tcgtcgggcg ccaccacatg
     4141 gcgcaccaac ccgggagcac cgtaggtcgt atcgctgatg tggacctggt gactgcccca
     4201 cgagatcagc ccggtgtcac cgatctggcc gcccgattcg gcgtagaacg gagtccggtc
     4261 caacacgatg gcgttgccga cgacgccgag cacgttcgcc tcgcactgct cgacctctcg
     4321 gccgacgaag acggtctcgc cgccctcgcg cagctgagcg aaggccgagg actcgctgtc
     4381 ggtggccacc ttgcccttct tggccgaggc cttggcgcga tctcgctgat cggccatcgc
     4441 ctcgtcgaat ccgtgctggt cgagttcgta gccggcgatc tcgacgaact cctgggtgac
     4501 ctcgagtgga aacccgaagg tgtcgtgcag tttgaaggcc accgcaccgt cgaggcgccc
     4561 gcccgtcggg agcttgtcga gttcgctgtc gagcaacgcg gagccggtct tgagggtctg
     4621 gcggaacttc gattcctcgc gttcgaccgt ggtgagcacg gcgtcggaat tggtccgcag
     4681 ttccgggtag gcgtcgccca tcacctcgat gcagcgagac acgagcccgg cgagaaccgg
     4741 ccgatcgacg ccggtgaggt acgcgaaacg gatcgcacgc cgaatgaccc gtcgaagcac
     4801 ataacctcgg tcctcgttgg agggaatcac gccgtcggac acgaggaagg tcatcgtgcg
     4861 ggtgtgatcg gccatcaggc gcaacgccac gtcggtgatc tcgccgtcac cgatgcggtg
     4921 acccgtcacc cgctcggcct cgccgacgag gtcggccaac acgtcggcgc tgtagagcga
     4981 atggctgccg ccgagcagcg ccaggatgcg ctccatgccg gcaccggtgt cgacattcgg
     5041 cgtcggaagg ttcgcgagca cgccgtcggc accgcggaag tactgcatga acacgaggtt
     5101 ccagatctcg acgaaacggt gttcggccgc ggggttggcc gggccgccgt cggggccgag
     5161 gtcgggtccg aagtcgtaga agatctccga gcttgggcca cacgggccgg tttcgcccat
     5221 ctcccagaag ttgtcggcgt cgaggcgttg gatgcgggcg cggggaacgc cgatcgaatc
     5281 ggcccagatg tcctcggcgt cgtcgtcgga ggtgtgcacc gtcacccaga tgcgatcgcc
     5341 gtcgaccccc agcaccgagg tcacgaactc ccaggccatc gcaatggcct cggccttgaa
     5401 gtagtcgccg aagctgaagt tgccgagcat ctcgaaaaac gagaggtggc gggggctgcg
     5461 accgatggcg tcgagatcgt tgtgcttgcc gccggcgcgg acgcactttt gcaccgacac
     5521 cgcccggggc gggttgaacg gcaccggttc ctcgccgagg aaatacggca cgaactgcat
     5581 catccccgag ttgttgaaca tcggggccga cgggtggtga gggatcagtc ccgcagacgg
     5641 aacgaccgtg tgtcccttct ccgcgaaata gttcgtgaag gcgaggcgaa gatcattggc
     5701 gcgcatgatg cgcacagcct atcgggcttg tttgcggcgc cggtcggggt tacgcgagcg
     5761 ccgtgctgag cctcgggtgg cttcggtgtc gcccgcgaca tcggcggcct tggcgtcggc
     5821 ggccttggcg tcgaatctgt cggcggcact cgccgcgaag ttcgacacgc tgttcgcgag
     5881 ggccgccgcg ttgtcggcgg ccgacgcggt ggtcgccgcc gcgctgccgg cgaagctgcg
     5941 aggcaacccg gcgcgcaggt cggcctcggt ctgacgcccc tcggcgatcc cctcgttcac
     6001 cgccgtgacc acccggtctt tcaggtcggt ggctttggtc ttggcgacgt cgacgacgtg
     6061 cgacggcgtg gccttggact gaatcgcctc gacctgttgt ttcaccttgc gttgacccca
     6121 cacggtggcc gccgaaccgg tcgcggcgcc gacgcccagc cagaacacgc gcttgaacat
     6181 caggactcct ccgacttggt caggcgacgg gcggcctttc gggttccgcg ggcgaccgcc
     6241 gcggttttga tgaccggatt cgacagcacc cgaacggccg acgcggtcgc gccgtccatc
     6301 gtctcggtca ccgaacctgc gacgtcgatg aggttctcga ggcgcgccac ctgacgtgct
     6361 gcggtgtccg ccgcggcctc gaggcgctcg gcggatggcc cggcgacctc ggtgaacgcg
     6421 tcgagctcct tgcgtaacga tcgcaccacg agtcgcaatt gaccgaccgt gatcgccagc
     6481 gcggcaatcg ccccaaggct cacgacggtc gtgacgatca agacgatgtc ggtcgcactc
     6541 atctccaaac accctagttc tcccagcctg ccccacccgt cccagcacct cgcctgcccc
     6601 accccagcct gttctccgat aaccagtggt cgtgatcacg accactggtt atcggagaac
     6661 aggcgttttc ggggatccgg gtcagtgcat gtaggccgac tccggcgggg gttccagctt
     6721 ggtggtcgac gcggcggcga tgaagtcgca cacctcgcgg gactttgcgg ccctcgacaa
     6781 cacgatcgct gcgtgggtga ggttgtgtcg cacctcgggc atcccgatga actcgctcgc
     6841 gtgggcggcc ccggttgcga tgatcaacgc cgaacggtcg gccggaccga tgtcctcgga
     6901 ggcgaagatg accattcgtc gggccaggaa tctcgggtcc tcgccgcggt cgagcatgtg
     6961 gttcaaccag gcggaagcct gctcggcgtt gccggatcgc atgtgcttga tgaacgcgct
     7021 cgcggcgtcg taatggtcgc cagcctgata gtgcgcgatc ccggcgccga gggcctcggt
     7081 cagcaggtcg aaggtgatct cgccgccgct gcccggcctg gacagatcgg ccgccgcctc
     7141 gagcccggtc aacagacgtc gtccgtcgcc ctccaccgac ccgatgaggt ggtcggccgc
     7201 atcctcggca accgagatcg gccggcgttt cgcgagctcc tcgacgccgc gatcgagcag
     7261 cgcccgcaga tcagcgtcac cgagcggctc gagtttgagc accgtgcaac ggctgagcag
     7321 cggccggttc actgtcgccc aggggctttc ggtggtcgct ccgatgaggg tc
//
LOCUS       DAOOMA010000045         7350 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_11960_length_7350_cov_3.350514, whole genome shotgun sequence.
ACCESSION   DAOOMA010000045 DAOOMA010000000
VERSION     DAOOMA010000045.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 7350)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7350)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7350
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11960_length_7350_cov_3.350514"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(111..911)
                     /gene="prcB"
                     /locus_tag="PLP95_02280"
     CDS             complement(111..911)
                     /gene="prcB"
                     /locus_tag="PLP95_02280"
                     /EC_number="3.4.25.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012228701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005839 - proteasome core complex
                     [Evidence IEA];
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0010498 - proteasomal protein catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proteasome subunit beta"
                     /protein_id="HPB44656.1"
                     /translation="MLPSFTPGEDPGPDFGELMKRVGLRSEPITVGPSDAAAITHGTT
                     CLAIRYADGVVIAGDRRATSGNWISHRTIEKVFPADRYSAVAIAGAAGPAIEMVRLFQ
                     LQLEHYEKVEGNHLSLEGKANQLSQMIRNHLPAAMSGMAVVPLFAGYDTKRGNGRLFQ
                     YDITGGRYEEHDYAATGSGSMHASTVAKLGFRPALSREEAVDLVIEALWQAADEDSAT
                     GGPDLIRKIFPVVATVTAQGYQRLEDEELETRSRYLIGRIESRRGGVS"
     gene            complement(917..1111)
                     /locus_tag="PLP95_02285"
     CDS             complement(917..1111)
                     /locus_tag="PLP95_02285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0010498 - proteasomal protein catabolic
                     process [Evidence IEA];
                     GO_process: GO:0019941 - modification-dependent protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquitin-like protein Pup"
                     /protein_id="HPB44657.1"
                     /translation="MAEREQIRKNAPTRTDDTDVDDLAPSEAGSRIKAELDDLLDEID
                     DVLETNAEDFVKSYIQKGGE"
     gene            complement(1231..2814)
                     /gene="dop"
                     /locus_tag="PLP95_02290"
     CDS             complement(1231..2814)
                     /gene="dop"
                     /locus_tag="PLP95_02290"
                     /EC_number="3.5.1.119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020379539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010498 - proteasomal protein catabolic
                     process [Evidence IEA];
                     GO_process: GO:0019941 - modification-dependent protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="depupylase/deamidase Dop"
                     /protein_id="HPB44658.1"
                     /translation="MVLPIVCGIETEYGIIVRSPDGAEPTSMSPMTASSILVSAYVDT
                     GLRQRGASTASGTSWDFHDETPSNDARVGDVEHPGTTPSGARPGGAGDISVSTFAPEV
                     EAHLVNTVLSNGSRFYVDHAHPELSTPECRTPREVVRYDAAGDEILRRAMTAANRQLP
                     GRREVVIYKNNSDGKGQSYGCHENYLLARSTPFDHLAAGATPHLVTRQIFCGAGKVGR
                     EADGVPASGDVFQISQRADFFEEAIGLETTLKRPVVNTRDEPHADASRYRRFHVIIGD
                     ANISEVATLLKVGTTALWLATIDAGLVTDPIRLAAPVSAVRTVSRDLTLREPLELANG
                     STMTALDIQWEIFRQVDAAASHGILESIGAEAGNEVLQRWHDVLATLERDPMELADTV
                     DWVAKYRLLDAYRQRHGLAWSNPRLAAMDLQYHDLRPERSLAARLGLQRICSDDEVAR
                     AVIEPPESTRAWFRGRSLAQFGDAVVSANWDSLVFDVGESALRRVPMMDPVKGTRAHV
                     EALFDGCEDAAELVRRLGA"
     gene            complement(2825..4933)
                     /locus_tag="PLP95_02295"
     CDS             complement(2825..4933)
                     /locus_tag="PLP95_02295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB44659.1"
                     /translation="MNTRRWAATIAAFMVVTTALSACDHPSIPIGSLDSVGLSTDGRA
                     IRLTGWAIDRDTASPILITAAVANGNRVSAAASTHRPDVGAAYPAAGATHGFDVAIAA
                     PPGRHTACVGVHNVGNGADVMLGCRTITVPSIDPFGSVDAFDITPTSVRVAGWAVDPD
                     AGGPIDVMVTSKVDGTIDDPQVAAAPAAHARPDVAAATRGPINSGYDLTMSFAEPGTH
                     TTCVVAINRALGANRELGCRRHVVADRRPTGFVSSVRIVGSEVTVAGTATDPDGAPVR
                     VEVAGRPAVVATDGRWQVTVNGLADGAHQFCATLRNVGTGSGIQRDRTLPCATAVIGA
                     LNVATTGVVGVTHAVRSSSSSLADVDRDAGVSTRLSDGSTLWLFGDSTAPNPDGSMRY
                     FVSGTAAWAPATASTSPADASDAQRVPYLLARPTESFGACPTSRAHKAMWPISAVTVP
                     NGDGTDRVLVYLANMCLGSAEDFEYKGVSIGEYHYNPASPPIHQPVQLRILEQNLFGR
                     RAYGTASVLAPDGYLYAYQCDHPSNPADYAGYGPCRVARVAPHRAHVRADYTYWDGAG
                     WTATDLSAPAGHLPMDAGAFVGYNAPASSFNVSRDDSRGLYVMVYSPWPGFIGQATIR
                     FAESPIGPWSAPVSVALPDCENTVGGQRRSCYAATPKLDHGAEGRLGLGFYDQATALS
                     FGRGQYFVTSAAVSVSRRSG"
     gene            complement(4973..6712)
                     /gene="arc"
                     /locus_tag="PLP95_02300"
     CDS             complement(4973..6712)
                     /gene="arc"
                     /locus_tag="PLP95_02300"
                     /EC_number="5.6.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015798341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000502 - proteasome complex [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0010498 - proteasomal protein catabolic
                     process [Evidence IEA];
                     GO_process: GO:0019941 - modification-dependent protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proteasome ATPase"
                     /protein_id="HPB44660.1"
                     /translation="MTEPNDSPEVETLRNEVAELHDEITLLQRRIQDAPKRVRTLEER
                     LLETKGQLAQAVAQNEKLTFTLREARDHIAALRDEVDKLTQPPSAYATLLELNDDDTA
                     DVMSSGRKMRVEVSPSVDPATLIAGAEVVLNESYAVVMVRRPDTVGEIVNLREVLEDG
                     TRAIVLGRADEERVATIGESLVGVPIRSGDSLLMDTRSMVLLEKLPRGEVDDLLLEEV
                     PDISYSDIGGLDDQIEQITDAVELPFLHQDLFNVYALPAPKGILLYGPPGCGKTLIAK
                     AVANSLSKKVAAATGRESASYFISVKGPELLNKYVGETERHIRDVFTRAREKAADGTP
                     VIIFFDEMESLFRTRGTGISSDMESTIVPQLLAEIDGVETLKNVIVIGASNREDLIDP
                     AILRPGRLDVKIKIGRPDRAAAAAVFGRYLTDDTPVDGVEIDSLGGGDRHKALLAMIE
                     GTVEEMYREDESSQFLEVTYQNGDKEIMYFKDFASGAMIENIVRRAKKLAIKRELVSG
                     TRGVRLSDLVTATHQEFKEHEDLPNTTNPDDWAKISGKKGERIVYIRTLIQQEDHFEG
                     GRAIEAVVTGQYL"
     gene            6954..7199
                     /locus_tag="PLP95_02305"
     CDS             6954..7199
                     /locus_tag="PLP95_02305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012228720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HPB44661.1"
                     /translation="MGMKVWIDQDLCTGDGLCEEIAPKVFKLLDDGLAYVVSPDGTVH
                     SDPGGAEGLAEVPDSEIEGTIESAEECPGECIFIEVD"
BASE COUNT         1187 a         2401 c         2444 g         1318 t
ORIGIN      
        1 gccgtcggca taggtgacgg ccacgagtga acgacctcgg gcgatgccct tgcgcgcgta
       61 gtcggcgcgg tccttcatca tctgttcggg agcgacgtag aacggagcgg tcatgacaca
      121 cctccccggc gggactcgat gcggccgatg aggtagcgac tgcgggtttc gagctcctcg
      181 tcctcgaggc gttgatagcc ctgcgcggtc accgtggcga cgaccgggaa aatcttgcgg
      241 atcaggtcgg ggccgccggt ggcggaatcc tcatcggcgg cctgccacag cgcctcgatg
      301 accagatcga cggcctcctc gcggctgagc gcggggcgaa acccgagctt ggccacggtc
      361 gaggcgtgca tcgaaccgga tccggtcgct gcgtagtcgt gttcctcgta gcgtccgccg
      421 gtgatgtcgt attggaacaa ccggccgttg ccgcgtttgg tgtcgtagcc cgcgaacagt
      481 ggcacgaccg ccatcccgct catcgccgcc ggaaggtgat tgcggatcat ctggctcagc
      541 tggttcgcct tgccctcgag gctcaggtgg ttgccttcga ccttttcgta gtgttcgagc
      601 tgcaactgga acaaccggac catctcgatc gccggccccg cggccccggc gatggccacc
      661 gccgaatagc gatcggcggg gaagaccttt tcgatcgtgc ggtggctgat ccagttgccc
      721 gaggtcgcac gtcgatcgcc ggcgatgacg accccatcgg catagcggat ggcgaggcag
      781 gtggtgccat gggtgatcgc cgcggcatcg ctcgggccaa cggtgatcgg ctcggagcga
      841 agcccgaccc gtttcatcag ctcaccgaaa tctgggccgg ggtcttcacc cggtgtgaaa
      901 ctcggcagca tttcggttac tcgccgccct tttggatgta ggacttcaca aaatcctccg
      961 cgttggtttc gaggacgtcg tcgatctcgt cgagcaggtc atcgagttcg gctttgatgc
     1021 gcgacccggc ctcgctggga gcgagatcgt cgacatcggt gtcgtcggtg cgagtgggag
     1081 cgttctttcg gatctgttcg cgttcggcca cagtcgttac ctccagaacc ttcaacgtag
     1141 cgtgatccgc cccgggttcc gacgcatggt cgtcggcccc gaccgctcgc cggtcgccca
     1201 ccgactgctc gccggtggtt cgccggtcgt tcaggcgccg aggcgacgga ccaactcggc
     1261 ggcatcctca cagccgtcga agagcgcctc gacatgggcc cgggtgcctt tgacggggtc
     1321 catcatcggg acccgccgca gcgccgactc gccgacatcg aacaccagcg aatcccagtt
     1381 ggcgctgacc accgcatctc cgaattgggc gagactacgg ccgcggaacc aggcgcgggt
     1441 cgactccggc ggctcgatga cggcccgggc gacctcgtcg tcgctgcaga tgcgttgcag
     1501 cccgagacgg gcggccagcg aacgttcggg gcgcaggtcg tggtactgca ggtccatcgc
     1561 tgcgaggcgg gggttcgacc aggcgagacc gtgtcgctgc cgataggcgt cgaggaggcg
     1621 gtatttcgcg acccaatcga ccgtgtcggc gagttccatc gggtcgcgtt cgagggtggc
     1681 caacacgtcg tgccatcgct gtagcacctc attgccggcc tcagccccga tcgactcgag
     1741 gatgccgtgg ctggcggcgg cgtcgacctg gcggaaaatt tcccattgga tgtccaacgc
     1801 cgtcatggtc gacccgttgg cgagttcgag cggttcacgc agcgtgaggt cccgggacac
     1861 ggttctgacc gcggacaccg gagcggcgag gcggatcgga tcggtgacca gtccggcgtc
     1921 gatcgtcgcc aaccacagcg cggtggtgcc gaccttcaag agcgtcgcga cctcgctgat
     1981 attggcgtct ccgatgatga cgtgaaatcg gcgatatcgc gacgcgtcgg cgtggggctc
     2041 gtcgcgggtg ttgacgacgg ggcgcttcag cgtcgtttcg agcccgatcg cctcttcgaa
     2101 aaaatccgct cgttgcgaaa tctgaaacac gtctccgctc gccggcacgc catcggcctc
     2161 gcgtccgacc ttgccggcac cgcagaagat ctgacgggtg accaggtgtg gggtggcgcc
     2221 ggcggccagg tggtcaaacg gggtggagcg cgccagcagg tagttctcgt ggcacccgta
     2281 gctctgcccc ttgccgtcgg agttgttctt gtagatgact acctcgcgac ggccgggaag
     2341 ttgacggttt gccgcggtca tcgcccggcg aagtatttcg tcacctgctg cgtcgtagcg
     2401 cacgacttcg cgcggggtgc ggcactcggg agtcgacagt tccgggtgag cgtggtcgac
     2461 gtagaaacgg cttccgttcg acagcaccgt gttgaccaga tgcgcctcca cctccggggc
     2521 gaaggtggac acgctgatgt cgccggcgcc tcccgggcgc gcaccactcg gagtcgtccc
     2581 cgggtgctcg acgtctccga cccgtgcgtc gttgctcggc gtctcgtcgt ggaaatccca
     2641 cgaggtgccg ctggcggtcg acgccccacg ctgtcgaaga ccggtgtcga cgtaggccga
     2701 gaccaggatc gacgaggccg tcatcgggct catcgaggtc ggctcggctc cgtcaggtga
     2761 tcgcacgatg atgccgtact cggtttcgat tccgcacacg atcggcaaca ccacgggttc
     2821 gaccctaccc gcttcgtcgc gaaaccgaga ccgccgcgct cgtcacgaag tactgtccgc
     2881 gaccgaaact cagcgcggtc gcctggtcat agaacccgag cccgagacgc ccctcggcgc
     2941 cgtggtcgag tttcggtgtc gccgcgtaac acgaccggcg ctgaccaccg acggtgtttt
     3001 cgcagtcggg cagcgcaacc gaaaccgggg cgctccacgg cccgattggc gactcggcga
     3061 aacggatggt cgcctgtccg atgaatcccg gccacggcga gtacaccatc acgtagagcc
     3121 cgcgcgaatc gtcccgagac acgttgaacg aggaggccgg agcgttgtag cccacaaagg
     3181 cgccggcgtc catcggcagg tggccggccg gggccgagag gtccgtcgcc gtccacccag
     3241 cgccgtccca gtaggtgtag tcggcccgga catgcgcgcg gtggggcgca actcgggcga
     3301 cgcggcacgg gccgtaaccc gcgtagtcgg cagggttcga cgggtgatcg cactggtacg
     3361 catacaggta cccgtcgggg gccaacaccg aagcggtccc ataggcacgc cgcccgaaga
     3421 ggttctgttc gaggatccgt agctgcacgg gctgatgaat cggcggcgat gccgggttgt
     3481 agtggtactc gccgatcgaa acgcccttgt actcaaaatc ctcagcgctg ccgagacaca
     3541 tgttcgccag gtacaccagc actcgatcgg ttccgtcacc gttcggcacc gtgaccgcgg
     3601 agatgggcca catcgccttg tgggcccggg aggtggggca tgcgccgaac gattcggtcg
     3661 gacgagcgag caggtacggg acccgctggg catccgacgc gtccgccgga gaggtcgacg
     3721 ccgtggcggg cgcccacgct gcggttccgc tcacgaaata ccgcatggaa ccgtcggggt
     3781 tcggggccgt ggaatcgccg aaaagccaaa gggtcgagcc gtcggacaga cgggtggaaa
     3841 ccccggcgtc gcgatcgaca tccgcgagcg aactcgatga cgagcgcacg gcgtgggtga
     3901 cgccgacgac cccggtcgtg gcgacgttga gcgcgccgat caccgcggtg gcacagggca
     3961 gggtccggtc gcgttggatt cccgaaccgg tgccgacgtt gcgcagcgtc gcgcagaact
     4021 gatgtgcgcc atcggcaagt ccattcacgg tcacctgcca gcggccgtcc gtcgcgacga
     4081 ccgcgggccg acccgccacc tccacccgga ccggcgctcc gtcgggatcg gtcgccgttc
     4141 ccgcaaccgt cacctccgat ccgacgatac gaaccgagga gacgaacccg gtcggccgtc
     4201 ggtcggccac gacgtgacgg cgacacccca actcacggtt ggcgccgagg gcccggttga
     4261 tcgccaccac acaggtcgtg tgggtgcccg gttcagcgaa cgacatcgtc aggtcgtagc
     4321 cggagttgat cggaccgcgg gtcgcggcgg cgacatcggg ccgtgcatgc gccgccgggg
     4381 ccgcggcgac ttgtggatcg tcgatggtgc cgtccacctt cgaggtcacc atgacgtcga
     4441 tggggcctcc ggcatcggga tcgaccgccc atcccgcaac ccgcaccgag gtcggcgtga
     4501 tgtcgaaggc gtcgaccgat ccgaacgggt cgatcgacgg caccgtgatc gtgcgacatc
     4561 ccagcatcac gtcggcgccg ttgcccacgt tgtggacgcc aacgcacgcg gtatggcggc
     4621 cgggcggcgc ggcgatggcg acgtcgaagc cgtgggtcgc tccggccgca gggtaggccg
     4681 caccgacatc gggccggtgc gtgctggccg cggccgagac ccgatttccg tttgccaccg
     4741 cggcggtgat caggatcggc gaggcggtgt cgcggtcgat cgcccaaccg gtgagccgga
     4801 tcgcacgacc gtctgtgctc aaccccaccg aatccaacga tccgatgggg atgctcgggt
     4861 gatcacacgc gctcaacgcc gtcgtgacca ccatgaacgc ggcgatcgtg gccgcccagc
     4921 gcctcgtgtt catcctcgcc tccgccgccg tcgtgtcagg gggttggccg ggtcacaggt
     4981 actgacccgt cacgaccgct tcgatcgccc ggccaccttc gaagtgatct tcttgttgga
     5041 tcagcgtgcg aatgtagacg atccgttcgc cctttttgcc ggagatcttc gcccagtcgt
     5101 cggggttggt ggtgttgggc aggtcttcgt gttccttgaa ttcctggtgc gtcgcggtca
     5161 ccaggtcgct gaggcgaacc cctcgagttc cggacacgag ctcgcgtttg atcgccaact
     5221 tcttcgcccg gcgcacgatg ttttcgatca tcgccccgga cgcgaagtcc ttgaagtaca
     5281 tgatctcttt gtcgccgttc tggtaggtca cctcgaggaa ctggctggat tcgtcctccc
     5341 ggtacatctc ctcgaccgtt ccttcgatca tcgccaggag tgccttgtgg cggtctcctc
     5401 caccgagcga atcgatctcc acgccgtcga cgggggtgtc gtcggtcagg taacggccga
     5461 acaccgcagc ggccgcggcg cggtcgggcc gaccgatttt gattttcaca tcgaggcgtc
     5521 ccggccgcag gatcgcgggg tcgatgagat cctcccggtt cgacgcgccg atgacgatca
     5581 cgttcttgag cgtctcgacc ccatcgatct cagcgaggag ctgcggcacg atcgtcgatt
     5641 ccatatcgct ggagatgccg gtgccccgcg tgcgaaacag cgactccatc tcatcgaaaa
     5701 agatgatgac gggggtcccg tccgccgcct tttcgcgggc gcgggtgaac acgtcgcgga
     5761 tgtgacgttc ggtctcgccg acgtacttgt tcaacagctc cgggcccttg acgctgatga
     5821 agtaactcgc agactcccga cccgtggccg cggcgacctt cttggacagc gagttggcca
     5881 cggccttggc gatgagtgtc ttgccgcaac cgggcgggcc gtacagcaaa atgcccttcg
     5941 gcgccggcaa ggcgtacacg ttgaacagat cctggtggag aaacggcagt tcgaccgcgt
     6001 cggtgatctg ttcgatctgg tcgtcgaggc cgccgatatc gctgtaggag atatcgggca
     6061 cctcctcgag caacaggtcg tcgacctcgc cgcgtggaag cttttcgagc agcaccatcg
     6121 agcgggtgtc catgaggagc gagtcgccgc tgcgtatcgg cacgccgacg agcgattcac
     6181 cgatggtcgc cacgcgttcc tcatcggcac ggcccaacac gatcgcccgg gttccatcct
     6241 cgagcacctc gcgcagattg acgatctcgc cgacggtgtc gggtcgacga accatcacca
     6301 ccgcgtagct ctcgttgagc acgacctcgg ccccggcgat gagcgtggcg ggatcgaccg
     6361 acgggctcac ctcgacgcgc atcttgcggc ccgagctcat cacgtcggcg gtgtcatcgt
     6421 cgttcaattc gagcagcgtg gcgtacgccg atggcggctg ggtcaacttg tcgacctcgt
     6481 cgcgcagcgc ggcgatgtgg tcgcgcgcct cacgcaaggt gaaggtgagc ttctcgttct
     6541 gggcgacggc ctgggcgagc tgtcccttgg tctccagcaa ccgttcttcg agggtccgca
     6601 cgcgcttcgg tgcgtcctgg atccgccgtt gcagcagcgt gatctcgtcg tgaagctccg
     6661 caacctcgtt gcgcagggtt tccacctcgg ggctgtcgtt cggttcggtc accgtccacc
     6721 tccctggagc tcgtggtcgt cggcgttggc ggttgcgact cgctgaggcc tcgccgtgac
     6781 gagaaccgtc cctcggcaag ctaacaccgt gccggcagaa tccaactcac cccgtcgaac
     6841 atgtcgattg tcacctcggc ggagttgccc gaaaccgtct tgcttgtggt tagaatcgat
     6901 cgcaggcgtc gggaccgaac acccgcaccg aacaatccga ggagcatgga actatgggta
     6961 tgaaggtctg gatcgatcag gatctgtgca ccggcgatgg cctttgtgag gaaatcgccc
     7021 cgaaggtgtt caaactgctc gacgacgggc tcgcatatgt cgtgagcccc gacggcacgg
     7081 tccactccga cccgggtggc gccgaagggc tagcagaggt tccggacagc gagatcgaag
     7141 gcacgatcga gtctgccgag gaatgccccg gcgagtgcat cttcatcgag gtcgactaac
     7201 cgactaccga gctcggtcgt cgtcatacga cgcgatgatg cccccgccga tggcgggggc
     7261 atcgtcgcgt ctgcgcacct gcctaccccg gttgccggcc acatgccccg tttgcctgtt
     7321 ctccgataac cagtggtcgt gatcacgacc
//
LOCUS       DAOOMA010000046         7349 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_11961_length_7349_cov_3.686592, whole genome shotgun sequence.
ACCESSION   DAOOMA010000046 DAOOMA010000000
VERSION     DAOOMA010000046.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 7349)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7349)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7349
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11961_length_7349_cov_3.686592"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..898)
                     /locus_tag="PLP95_02310"
     CDS             complement(<1..898)
                     /locus_tag="PLP95_02310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="HtaA domain-containing protein"
                     /protein_id="HPB44662.1"
                     /translation="MTKPTTTKARLASTPTPMRVRRPARALAMLTTVALGAGLIAFTD
                     TAGAAPLNITDATFEWSVSEEANTGSFNGQCNFMSAGVSDGYAGTYKATDGNATVLKL
                     NSANEYVPISDYTTRCKDKDGNNVTAAGARRLGQKVRYTKGVGTADPVTGETSIQFTG
                     TFSVNYYGTLTPFWFINPRVEVDASGNGKLVASMAGYASSIDDPDVRELINPVANVTV
                     ATFDTNSKNNTGFVATPHWAGVEYNDGEVPQIRVFPGWGSWPLPMIKMMERLGLGAYW
                     YTTGSAADARKPGAPLTVGFGAT"
     gene            complement(985..2154)
                     /locus_tag="PLP95_02315"
     CDS             complement(985..2154)
                     /locus_tag="PLP95_02315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB44663.1"
                     /translation="MIRSTSRIVAAFALTASVTVASVGLGGDAPVASAATSRVKAQVQ
                     QCGNITAASQLTVDKTTLPATGTTEITVTGRSYQAPLRDCTKPVFGGIYVFFGWVKPG
                     GQWGPSWKSSNSTDGLFGYTFSYPGEGGGDDTRDDGTGTVRLVAFSPGGESGDATPFH
                     MDGSGNWQTTLTVRGSSYHFKNVRTDEEFTVDCLKVQCGVLTIGGHGITSRPNEQFTP
                     INFTTGGGQVVVPPANPNPGVGGSGSAASGTGGSGSGGSGSKGAASNSGSNSGSGSAS
                     GGVSGGASGGDSGTDAASGGDPASDPASESATVGEAGTLGEAPDASVAEATEEREALD
                     SSNTAAKARVQDFGGDDSGGGSGIVIGVAVALVAAVGATGAFLARKKRQPSPDAG"
     gene            2376..3281
                     /locus_tag="PLP95_02320"
     CDS             2376..3281
                     /locus_tag="PLP95_02320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB44664.1"
                     /translation="MKIFSRRRAVAVAALATAGLVGACTPPESGTPDPNVKSVSNAVF
                     EWEISREANNAAFAPGQFNWWNAGTAAATNDATYNNATHGNATVLKKNASGTFVPIGS
                     ESAVSFANRTKKGNGAAVTTGNSNFLDQKVRYTAGTGKVNTATGVATISWSGTFTINF
                     YGIYVPFQVANPKLTVDASGKGTLTATLSGIAKDQSDPEAPGVTLPKTNVTLAEVPNF
                     YSSGSIATGASAAQPNYLGKVVTTHPDAAPQTASGTAYHGSWPQNFVNFQNATGLSSY
                     WYSSGGAADALKVQLPFSVNYSIAG"
     gene            complement(3480..5204)
                     /locus_tag="PLP95_02325"
     CDS             complement(3480..5204)
                     /locus_tag="PLP95_02325"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: protein motif:HMM:NF006625.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HPB44665.1"
                     /translation="MRWSKLFIPTLKETPGDAEAISHKLLIRAGFVRQLHAGHYSLLP
                     LGLRSHNKVAAIIREEMDAIGDQEFSLPAMHPAALWQESGRWETMGAEMFRLEDRRGN
                     ELALGMTHEEVFTTLAREITSYRDLPQSWYQVQTKFRDEPRPKAGLLRVREFFMKDAY
                     SFDLDTAGLDASFEAHRGAYQRIFDRLGLPAFPVMASSGAMGGGQSLEFMVPSAAGED
                     DVIICPNCDYRANVERATSALKEIGPKDGPDEVERFETPGVVTIEQLAKFAGGAGAVH
                     QIKTLVYVIDGEAVVVILRGDHNLNEQKLADLTGGIGIRPAGEDEILALLGAKPGSLG
                     AVGVTGPRIYADLALQGRTRMTTGANDDGWHLRGVDLARDISVSEWADLRTVTKGEPC
                     IECGAKLTVERCVETGHIFKLGTKYSDAMGATVLDPNGDLVPLIMGCYGIGVGRALAV
                     AIETHNDDKGMVLPVTVAPFEVCITIVKVDDPESVRSAETIYNELRNLGIDVILDDRD
                     ERPGVKFADSELVGIPFRITVGPKALAAGNVEFTVRDGRTSSEVPLGEVVSHTTELVH
                     AGRRLGMG"
     gene            5293..6540
                     /locus_tag="PLP95_02330"
     CDS             5293..6540
                     /locus_tag="PLP95_02330"
                     /inference="COORDINATES: protein motif:HMM:TIGR00707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006525 - arginine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylornithine/succinylornithine family
                     transaminase"
                     /protein_id="HPB44666.1"
                     /translation="MTNAANSPLMNTYASPAVTFTRGEGSWLFDDEGRRYLDLLSGLA
                     VTSLGHAHPELVAAVSEQASKLWHVSNLYGNEHNEALARELDRLITGGGEVGDPVGND
                     PVGNGPVGNGPVGKVFFTNSGAEANECAIKLARRWAGYGRHVVVSAFGSFHGRTLATL
                     HATGQPQKHEAFQPLPEGFRHAAFDDLDDLERQIDDTTAAVLLEVVQGEGGVNPVSEA
                     YLRGVAELCAERNLLLMLDEVQTGLGRCGEWFAHHRYGVKPDVVTVAKALGNGMPIGA
                     CWARSEVADAFVPGDHGTTYGGQPLVAATARKVLEIMRRDEVVSRAREQGAAIIAGLE
                     GRPGVAAVRGLGLLIAVELDQNGARALGAPAVASKLLERGVIVNAVTPTALRLAPSLL
                     ITDDEVRLAVASIVGVLGEMLES"
     gene            6543..7307
                     /locus_tag="PLP95_02335"
     CDS             6543..7307
                     /locus_tag="PLP95_02335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020379340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HPB44667.1"
                     /translation="MVRAATCNAPRSTSVVMPAFNEEATIEEIVGRVLASPWTAELII
                     VDDGSRDATVAIATKLAEADDRVRVVCQPVNRGKGAALARGFAEATAEFVVVQDADLE
                     YDPAEYDDLLAPLAEGRADVVFGSRFLAGRPHRVLYFWHSVGNRFLTLMSNMFTDLNL
                     TDMETCYKAFRREVIAGIRIEENRFGFEPEITAKVAAGGWRIYKVGISYDGRTYADGK
                     KIGWKDGCRALWCIVKYSPIGTKVRRLVAKLTGRGR"
BASE COUNT         1195 a         2334 c         2557 g         1263 t
ORIGIN      
        1 tagtcgcgcc gaatcccacc gtgagcggag cccccggctt gcgggcatcg gcggcacttc
       61 cggtggtgta ccagtacgcc ccgagcccga ggcgttccat catcttgatc atcggcagcg
      121 gccaggaacc ccagccggga aagacccgga tctggggaac ctcgccgtcg ttgtactcga
      181 cgccggccca gtgcggggtc gccacaaacc ccgtgttgtt cttcgagttg gtgtcgaacg
      241 tcgcaacggt cacattggcg accgggttga tgagttcgcg gacatcgggg tcgtcgatcg
      301 aggaggcgta tccggccatg gaggcgacca gcttgccgtt tccggaggcg tcgacttcga
      361 cgcgggggtt gatgaaccag aacggcgtca gggtgccgta gtagttgacc gagaaggtgc
      421 cggtgaactg gatcgacgtc tcgccggtga cgggatcggc agtcccgact cccttggtgt
      481 agcgcacctt ttgaccgaga cggcgagcac ccgccgccgt gacgttattg ccgtctttgt
      541 ccttgcagcg cgtcgtgtag tccgagatcg ggacgtactc gttcgcggag ttcaacttga
      601 gcaccgtggc gttaccgtcg gtcgccttgt aggtgcccgc atatccgtcg gacacccccg
      661 ccgacatgaa gttgcactgt ccgttgaacg aacccgtgtt ggcttcctcg ctgaccgacc
      721 actcgaacgt cgcgtcggtg atgttgaggg gcgccgcacc ggcggtgtcg gtgaaggcga
      781 tcagcccagc gccgagtgcg acggtggtca gcatggccag ggcccgggcc gggcgtcgaa
      841 cccgcatcgg cgttggcgtg ctggccagtc gggccttggt cgttgttggc ttcgtcattc
      901 gtgtgctcct ggagatggcg gagatcggtg tcggtgtctg gagtgtctgg agtgtctggc
      961 ggttgagttc cgttgccggg cggatcagcc ggcgtcggga gaaggttggc gcttcttgcg
     1021 tgcgaggaat gctcctgtgg caccaaccgc tgcaacgagc gccacggcga cgccgatgac
     1081 gatgcccgag cccccgcccg agtcgtcgcc gccgaaatct tgaacccggg cctttgccgc
     1141 ggtgttggaa ctgtcgaggg cctcgcgctc ctcggttgct tcggcgacgc tggcatcggg
     1201 tgcctccccg agggtgccgg cttcgccaac ggtcgccgat tccgatgcgg ggtcggacgc
     1261 tgggtcgccg ccgctcgccg cgtcggtgcc ggaatcgccg cccgaggcac caccggaaac
     1321 gccgcccgag gcactgcccg agccgctgtt ggaaccgctg ttcgatgccg cgcccttcga
     1381 gcccgacccg cccgagcccg acccgcccgt gcccgaagca gccgaacccg acccgccgac
     1441 accagggttc ggattcgcag gcggcacgac gacctggccg ccgccggtgg tgaagttgat
     1501 cggcgtgaac tgttcgtttg gacggctcgt gatgccgtgt ccgccgatgg tcaacacgcc
     1561 gcactgcacc ttgaggcagt cgacggtgaa ctcctcgtcg gttcggacgt tcttgaagtg
     1621 gtacgacgat cctcgaacgg tcagcgtggt ctgccagttg ccagagccgt ccatgtgaaa
     1681 gggggtggcg tcgccggatt cgccgccggg ggagaaggcg acgagtcgca cggttccggt
     1741 gccgtcgtcg cgggtgtcgt cgccgccgcc ctcgcccggg tagctgaagg tgtagccgaa
     1801 caggccgtcg gtgctgttcg aggacttcca ggacggaccc cactggcccc cgggcttgac
     1861 ccaaccgaag aacacgtaga tgccgccgaa gaccggcttg gtgcagtcgc gcagcggcgc
     1921 ctggtagctc cgtccggtga ccgtgatctc ggtggttccg gtcgcgggca gcgtggtctt
     1981 gtcgacggtg agctgggacg ccgcggtgat gttgccgcac tgctggacct gtgccttgac
     2041 ccgtgatgtc gcggccgatg ccacaggcgc atcaccgccc agtccgacgg aggcgacggt
     2101 gacggacgcg gtgagcgcga acgccgcgac gattcgagag gtcgatcgga tcatgcggcg
     2161 atgttaggta cgccatacat cgaatggcaa gtcgctcgtc gcaaccgtcg tggcgtggtg
     2221 ttcgcggttg tttgggcccg tcgccgatcg tcggtagccg ccgctccagt ctggcgtagg
     2281 ccgtgctatc tcgatatggt aggtcaacct catatgcggc aggccagcgt gtgagcgctc
     2341 ggctgccgac caatccgtga aggagaagca tcgcaatgaa gatcttcagt cgccgacgtg
     2401 cagtggccgt ggccgccctg gccaccgctg gcttggtcgg agcctgcacc ccaccggagt
     2461 cgggaacgcc ggatccgaac gtcaagtcgg tcagcaacgc tgtcttcgaa tgggagatca
     2521 gtcgcgaggc caacaacgcg gccttcgccc ccggtcagtt caactggtgg aacgccggca
     2581 cggccgccgc caccaacgac gccacctaca acaacgccac ccacggcaac gcgaccgtgc
     2641 tgaagaagaa tgcctcggga accttcgtgc cgatcgggtc ggagtctgcg gtgtcgttcg
     2701 ccaaccgcac caagaagggc aatggcgcgg cggtgacgac gggcaactcg aacttcctcg
     2761 accagaaggt tcgctacacg gccggaaccg gcaaggtcaa caccgcaacc ggcgtggcga
     2821 cgatctcctg gagcggcacc ttcaccatca acttctacgg catctatgtc ccgttccagg
     2881 ttgccaaccc gaagctgacc gtcgatgcct ccggcaaggg gaccctgacc gcgaccctca
     2941 gcggcatcgc caaggaccag agcgatccgg aagcacccgg agtcaccctg ccgaagacca
     3001 acgtcaccct tgccgaggtc ccgaacttct acagctcggg ctcgatcgcg accggagctt
     3061 cggccgctca gcccaactac ctgggcaagg tcgtgacgac ccaccccgat gccgcgccgc
     3121 agaccgcttc gggcacggcg taccacggct cgtggccgca gaacttcgtg aacttccaga
     3181 acgcgacggg tctcagctcg tactggtact cgagtggcgg cgccgccgac gcactcaagg
     3241 tgcagcttcc gttctcggtg aactactcga tcgccggctg agcgcagggg ttggctggcg
     3301 acgagccgac ccggtcgctg aacgaatcaa caaacacgaa cgaggcgcac tcaccgctga
     3361 aggtgagtgc gcctcgttcg cgttgtggcg ttgatgttct ggcgaccagt ccaggcgggg
     3421 tcagtccagg cggggtcagt ccagacggtt caatccaggc ggggtcagtc caggcggggt
     3481 cagcccatgc cgaggcgacg gccggcgtgc accagctcgg tcgtgtggga gacgacctcc
     3541 ccgagcggca cttcgctgga ggtgcggccg tctcgaaccg tgaactcgac gttgccggcg
     3601 gcgagcgcct tcggcccgac cgtgatccgg aacggaatgc cgacgagttc ggagtctgcg
     3661 aacttcaccc cggggcgttc gtcgcggtcg tcgaggatca cgtcgatgcc caggttgcgc
     3721 agttcgttat agatcgtctc ggcggagcga accgactcgg ggtcgtcgac cttgacgatc
     3781 gtgatgcaca cctcgaaggg ggccacggtg accggcagga ccatgccctt gtcgtcgttg
     3841 tgggtttcga tggcgaccgc cagcgcacgg ccgaccccga tcccgtagca ccccatgatg
     3901 agcggcacga gatcgccgtt ggggtcgaga accgtggcgc ccatggcatc ggagtacttc
     3961 gtgcccagct tgaagatgtg cccggtttcc acgcaccgct cgaccgtgag tttggcgccg
     4021 cattcgatgc acggctcgcc cttggtgacg gtgcgtagat cggcccactc gctcaccgag
     4081 atgtcgcggg cgaggtccac gccgcgaagg tgccaaccgt cgtcgttggc gccggtggtc
     4141 atccgggtgc ggccctgcaa cgcgaggtcc gcgtagatgc gcggcccggt gacgccgacc
     4201 gcgccgaggg agccgggttt ggccccgagc agtgcgagga tctcgtcctc gccggcggga
     4261 cgaatgccga tcccaccagt gaggtcggcc agcttctgtt cgttcaggtt gtggtcaccg
     4321 cgcaggatca ccacgaccgc ctcgccgtcg atgacgtaga cgagggtctt gatctggtgc
     4381 acggccccgg ccccaccggc gaacttcgcg agctgttcga tcgtgacgac gccaggggtc
     4441 tcgaaacgct cgacctcatc agggccgtcc ttgggtccga tttccttcag cgccgaggtg
     4501 gcccgttcaa cattggcgcg gtaatcgcag tttgggcaga tgatgacatc gtcctcgccg
     4561 gcggccgagg gaaccatgaa ctcgaggctc tggcccccgc ccatggcgcc ggaactcgcc
     4621 atgacgggaa acgccggcag gccgaggcgg tcgaagatgc gctgataggc gccacggtga
     4681 gcctcgaacg acgcgtcgag gccggcggtg tcgaggtcga agctgtaggc gtccttcatg
     4741 aagaactccc gaacccgcaa cagtccggcc ttgggccggg gctcgtcgcg gaacttcgtc
     4801 tgcacctggt accaggactg ggggaggtcc cggtaggagg tgatctcgcg ggcgagggtc
     4861 gtgaagacct cctcgtgggt catgccgagc gccaattcgt tgccgcgtcg gtcctcgagg
     4921 cggaacatct cggcgcccat ggtctcccag cgccccgact cctgccacag tgcggcgggg
     4981 tgcatcgcgg gcagcgagaa ctcctggtcg ccgatggcgt ccatctcctc ccggatgatc
     5041 gccgcgacct tgttgtggct ccgcaggccc agcggcagaa gggagtagtg gccggcgtgg
     5101 agttgccgca cgaaaccggc acggatgagc agcttgtgac tgatcgcctc ggcgtcgccc
     5161 ggggtttcct tcagggttgg gatgaacagc ttcgaccagc gcataggggg cgagactatc
     5221 cggcagacac ccgtggcccg atcatgaata actatgcagg ggagtgcaca ttttcgacta
     5281 gcgtcggtcg ccatgacgaa cgccgcgaac tccccgttga tgaacaccta tgcgtcgccg
     5341 gcggtcacct ttacccgcgg ggagggttcg tggctcttcg acgacgaggg gcggcgttac
     5401 ctcgacttgt tgagcggcct ggcggtgacc tcgctcggcc atgcgcatcc tgaactcgtc
     5461 gccgcggtga gtgaacaggc ctcgaagctg tggcacgtct cgaacctgta cggcaacgaa
     5521 cacaacgaag cgctcgcccg agaactcgac cggctgatca ccggtggcgg agaggtggga
     5581 gatcctgtcg gcaacgatcc tgtcggcaac ggtcccgtcg gcaacggtcc cgtcggcaag
     5641 gtgttcttca cgaactccgg agccgaggcg aatgagtgcg cgatcaagct cgcccggagg
     5701 tgggcgggat acggacgaca cgtcgtggtc agtgccttcg gatcgttcca cggtcgcacg
     5761 ctcgccaccc tccacgcgac cggtcagccg caaaagcacg aggccttcca gccgcttccc
     5821 gaggggtttc gccacgcggc gttcgacgac ctcgacgacc tcgagcgtca gatcgatgac
     5881 acgaccgccg ccgtgctgct ggaggtcgtg cagggtgaag gcggcgtgaa cccggtgtcg
     5941 gaggcgtacc tgcggggggt cgccgagctg tgcgccgaac gcaacctgtt gctgatgctc
     6001 gacgaggtgc agacgggcct cggacgctgc ggtgagtggt tcgcgcatca ccgctacggg
     6061 gtgaagcccg atgtggtgac cgtggccaag gcactcggca acggcatgcc gatcggggcg
     6121 tgctgggccc gctcggaggt ggccgatgcg ttcgtgcccg gcgatcacgg cacgacctat
     6181 ggaggacagc cgctcgtggc tgcgacggcc cgcaaggtgc ttgagatcat gcggcgagac
     6241 gaggtggtga gccgtgcccg cgaacagggc gcggcgatca tcgcgggcct cgaagggcgc
     6301 ccgggggtcg ccgccgtgcg cggactgggt ctgctcatcg ccgtcgaact cgaccagaac
     6361 ggcgccaggg cgctgggcgc cccggcggtc gcgtcgaagt tgttggagcg tggggtgatc
     6421 gtcaacgcgg tcaccccgac cgcgctgcgg ttggcaccga gcctgctcat caccgacgac
     6481 gaggtgcgcc tcgcagtggc gtcgatcgtc ggggtgctcg gcgagatgct tgaatcgtga
     6541 ccatggttcg agcggccaca tgtaacgcac cacgttcgac atcggtggtg atgcccgcgt
     6601 tcaacgagga ggcgacgatc gaggagatcg tcggccgggt gctggcgtcg ccgtggaccg
     6661 ccgagctgat catcgtcgac gacggttccc gcgacgcaac ggtggcgatc gcgacgaagc
     6721 tcgccgaggc cgacgatcgg gtgcgggtcg tgtgccagcc cgtcaaccgg ggcaagggcg
     6781 cagcgttggc ccgcgggttc gccgaggcaa ccgccgaatt cgtcgtcgtg caggacgccg
     6841 acctcgaata cgaccccgcc gagtacgacg accttctggc gccgctggcg gaggggcgcg
     6901 cggatgtcgt gttcgggtcg aggttcctgg ccggtcggcc gcaccgggtg ttgtatttct
     6961 ggcacagcgt cggcaaccgg ttcctgaccc tgatgtcgaa catgttcacc gacctgaacc
     7021 tcaccgacat ggagacctgc tacaaggcct ttcgtcgaga ggtgatcgcc ggcatccgga
     7081 tcgaggagaa tcgcttcggg ttcgagccgg agatcaccgc caaggtggcg gcgggcggtt
     7141 ggaggatcta caaggtcggc atttcctacg acggccggac ctacgctgat ggcaagaaga
     7201 tcggttggaa ggacggttgt cgggcgctgt ggtgcatcgt gaagtactcg ccgatcggaa
     7261 ccaaggtgcg tcgcctggtg gcgaagctga cgggcagggg tcggtagggc gcgggtcgcc
     7321 tgggagcgat tcggtagggc gcgggtcgt
//
LOCUS       DAOOMA010000047         7291 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_12097_length_7291_cov_3.950111, whole genome shotgun sequence.
ACCESSION   DAOOMA010000047 DAOOMA010000000
VERSION     DAOOMA010000047.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 7291)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7291)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7291
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12097_length_7291_cov_3.950111"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..413)
                     /locus_tag="PLP95_02340"
     CDS             complement(<1..413)
                     /locus_tag="PLP95_02340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB44668.1"
                     /translation="MTPEALLAAAGRRVRLVRVGRALMAAAPFVACALVVGLAIAWWV
                     PWGWLPLAVWVGAATTLFWVAFSAWHAALDPASVAAIADAHLDSHDALGAYLQFAGAG
                     SLGSLGADSPFLEPIRLRAERTAEGSNAATAVPTSL"
     gene            complement(410..1303)
                     /locus_tag="PLP95_02345"
     CDS             complement(410..1303)
                     /locus_tag="PLP95_02345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB44669.1"
                     /translation="MRSLLSNPLMRLEYLRRFRTATAAWTIPLLAILPIIGVSLIYGV
                     SNTASGFMAFDSMGNEIPTADLNFPTNLQATSQIDIGGVSLLIGTASVLTALLGLLVP
                     AIVGASIAGERQGDTLQPLQLTRIRPIDIVIGKLVGSLGYVLFLLVCLAPILVVPYLA
                     GGLSVSTIALTYLVLVAFAVELGSVSILASSRARRPVTAVIMALIANVALVAGPWIVA
                     GMIEIARFDSGPIEPQVSRVVWLSPITLFSIVGRVGDTELPGWWTTTDQIATVVCWAA
                     VITVSIVLARRGVRAPVDKDR"
     gene            complement(1303..2193)
                     /locus_tag="PLP95_02350"
     CDS             complement(1303..2193)
                     /locus_tag="PLP95_02350"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPB44670.1"
                     /translation="MTEAPISPAEAGRSGIAIRTESLTKTYGTNRALDSLSVQIESGT
                     VCGLIGPNGAGKSTLMSILATLLRPTSGSAEIFGIPLTDTEAIRPIIGYVPDVLGMYS
                     GLTVQEYLDFFAEAYRLPRDTRGATIEGLLELVDLSSKRYADVNTLSRGMKQRIGLAR
                     GLIHQPRLLILDEPASGLDPRARIELRDIINHLRELGMTVLISSHILGELEEMCTHGL
                     IVEQGRLVGFESLADEPTRSVEVRLLDGSTKRFEVPDLAAQRDLIRSLIHEGFEVVAV
                     TEVSTRLEDRFLRLTEGQVN"
     gene            complement(2190..>3182)
                     /locus_tag="PLP95_02355"
     CDS             complement(2190..>3182)
                     /locus_tag="PLP95_02355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB44671.1"
                     /translation="GNLDVARPVIYGRQSVSPLGSTHAAITATAGTAQTRLPQGWNVT
                     GSSNSVVVDGDKVTTRGEGTGWEPLVAGSFELDETSPITLVVTPAEGTLVHVAVTNHT
                     DSPLVAAEVSGFGRSRSFDRVRPGETVTLDFETAQDIDAWAKPFGWGLATSDCAMMCD
                     SNVNFGGFPIIDNMVTNGVVRVAGYLADDDDTFGQDRSRLDVVAATATVSGWSGPVDP
                     DAAHAAFIVEPLGGTRDGWSAYRITANASVATARCSIADFVDDVAIWDEENPHWAAIP
                     FGVEGLSERYVDPIGVRTYDLGAAEPGWQRIIRFRAGEFQIQSSSNHILCGVQP"
     assembly_gap    3183..3192
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3193..4296)
                     /locus_tag="PLP95_02360"
     CDS             complement(<3193..4296)
                     /locus_tag="PLP95_02360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB44672.1"
                     /translation="MGRPKLLIIAAACFAAAISTTSITAVPASASLSGQRQLEPQPAE
                     TTVPISPAGSEDRGQPNPADAVADAGIDVAATVGLNNRYGTNNPLPVRIEVTSDRSRQ
                     VEVVVESNVGDTAYRLDLNRDATAFIDHVGLTIGLPTAIVVREDGRRIARVPLQPMKA
                     SGSLVGVTSNLAPDTAQTIDLFDNTFSATIDRVDAEWLKRPAALEHLGELVVSTAELD
                     GFDDGLKRRIGHWVWAGGRLVIDGVAPAHDPVFDQPIDATEAQRGAVQIRFVGDAAVR
                     GDWRNVVQPQLDTGTAIGPEDQWFGDVGNDLSWRGLIETERASTSTLLGLLALSMLLI
                     GPALWFVLRKTGRPQFMWFAAPLLALAIGGLVVM"
     gene            complement(4373..5989)
                     /locus_tag="PLP95_02365"
     CDS             complement(4373..5989)
                     /locus_tag="PLP95_02365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020376966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HPB44673.1"
                     /translation="MLGTMMDFPLALPHLFQRAEQLFAKKEIITSSPKGIERTTYGEW
                     ADRSRRLGSALDTLGISPGGRVGTFGWNTARHLELYFAVPCSGRVCHTINLRLFPEQL
                     LFIVNHAEDEVLFIDNSIAPLAWPALEHFKTVKHVVVMNDTGAPIPTEAPNGIVVHDY
                     EELIANESPMQWNITDENTAAAMMYTSGTTGNPKGVVYTHRSIVLHTFAAMMADVVGM
                     RESDVVLPVVPMFHANAWGLAHAAVATGATLIMPGPDLTPPSLVKLMEDYRVTLAAGV
                     PTIWMGVLNAIDGHDVSSLRLIPCGGSAVPPALSEGFRAKIGLPIFQAWGMTETSPVA
                     AATLIKSYLEDTPELRATVGLVVPGVEFRVTEPNTIDELPWDGESRGELQVRGPWVTG
                     TYYNDLRSAESFTTDGWLRTGDVATVDSEGYIRLVDRTKDLVKSGGEWISSVDLENEI
                     MAHPDVVEAAVIGIAHPKWDERPLACVVLAEGSELTEAELLEWLAPRVAKWWLPDGVE
                     FIDEVPKTSVGKFSKKDLRDRFADFRWPES"
     gene            6086..6850
                     /locus_tag="PLP95_02370"
     CDS             6086..6850
                     /locus_tag="PLP95_02370"
                     /inference="COORDINATES: protein motif:HMM:NF024304.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HPB44674.1"
                     /translation="MAETPPPTPPPTSPTTAASTDSTPPTPEVGSKVISPTPATTIRN
                     AKRARLDREEAYAILDDDVVAHVGFVLDAQPFVIPMVYGRRGDSLFLHGSVATRIMRA
                     LDAGVSACVTVTKLDGLVLARSHFHHSVNYRCVVALGTARRLRDDAAMDAFDVIMEHL
                     MPGRVEEARRANRPEQRQTMVIEFPLVELSVKVRDGGPVDDEVDLPDPDELSDLWAGV
                     VPLRVVAGIPIADEFTSPNAPLPSAVATGATRWPAG"
     gene            complement(6976..>7291)
                     /locus_tag="PLP95_02375"
     CDS             complement(6976..>7291)
                     /locus_tag="PLP95_02375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB44675.1"
                     /translation="LEALAESDLVVIGTWTDGLIIAGHRPGGAGNIARHLPEIWDKPV
                     YTFVTYAVRPGNVLRGFRRLLESKGAKVLGGLELGRRHLDSDAIDFAEQVLRDSKSLQ
                     SA"
BASE COUNT         1256 a         2355 c         2491 g         1179 t
ORIGIN      
        1 agcgaggtgg gtacggcggt tgcagcgttc gacccctcgg ccgttcgttc ggcccgaaga
       61 cgaatcggct cgaggaacgg gctgtcggcc cccagactcc cgagcgagcc cgctcctgcg
      121 aactggaggt atgccccgag cgcatcgtgg gaatcgaggt gtgcatcggc gatcgccgcg
      181 accgaggccg gatcgagtgc cgcgtgccac gcggagaacg cgacccaaaa cagtgtggtt
      241 gcggcaccga cccacaccgc gagcggaagc caaccccacg gcacccacca tgcgatcgcc
      301 aacccgacca ccagcgcgca ggccacgaaa ggagcggctg ccatcagcgc tcgcccgact
      361 cgaacgagac gaacccggcg gccggccgcg gcgagcagtg cctcgggagt catcggtcct
      421 tgtcgaccgg ggcgcgaact ccgcgcctcg ccaacacgat cgaaacggtg atcacggcgg
      481 cccagcacac cacggtcgcg atctggtccg tcgtcgtcca ccagccgggc aactcggtgt
      541 cgccgacccg gccgacgatg ctgaacaggg tgatgggcga caaccagacc acccgcgaga
      601 cctgaggctc gatggggccg gaatcgaatc gcgctatttc gatcatccct gcgacgatcc
      661 acgggccggc gacgagcgcc acattcgcga tcaacgccat gatcaccgcg gtgacgggtc
      721 gacgtgctct gctgctggcg aggatcgaca cggacccgag ttcgacggcg aacgcgacga
      781 gtacgaggta ggtcaaggcg atcgtcgaca ccgacaaacc accggcgagg tagggaacga
      841 ccaggatcgg cgcgaggcac accaggagga acaggacgta tccgagcgag ccgaccagct
      901 tgccgatgac gatgtcgatc ggccggatcc gggtgagctg cagcggctgc aacgtgtcgc
      961 cctgccgttc gccggcaatc gacgctccga cgatcgccgg caccagcaac ccgagcagag
     1021 ccgtcagcac gctcgcggtt ccgatcagga ggctcacccc accgatgtcg atctgcgagg
     1081 tggcctgaag gttcgtggga aagttgagat ctgcggtcgg gatctcgtta cccatcgagt
     1141 cgaaggccat gaaccctgat gccgtgttgg acacgccgta gatcagcgac acgccgatga
     1201 tcggaagtat cgcaagcaag gggatcgtcc acgccgcggt ggcggttcgg aagcgtcgga
     1261 ggtactccag tcgcatcaag gggttggaca gcagcgaccg catcagttga cctgcccttc
     1321 ggtgagacgg aggaaccggt cttcgagccg agtcgacacc tcggtgacag cgaccacctc
     1381 gaaaccctcg tggatgaggc ttcgaatgag gtcgcgttgt gcggcgaggt cgggcacctc
     1441 gaagcgcttc gtcgagccgt cgaggagccg tacctcgacc gatcgggtgg gttcgtcagc
     1501 gagcgactcg aatccgacga gtcggccttg ttcgacgatc aacccgtggg tgcacatctc
     1561 ctccagctcg ccgaggatgt gcgaactgat gagcacggtc atgccgagtt cgcgcaggtg
     1621 attgatgatg tcgcgcagct cgattcgagc ccgcggatcg agcccggagg cgggctcgtc
     1681 gaggatcaac agtcgtggct gatggatgag cccacgggcg aggccgatgc gctgcttcat
     1741 gccgcgcgag agggtgttga cgtcggcgta gcgcttcgaa ctcaggtcga cgagttccag
     1801 gaggccctcg atcgtggcac ctcgagtgtc gcgggggagc cggtacgcct cggcgaagaa
     1861 gtcgaggtac tcctgcacgg tcagcccgga atacatgccc aacacgtcgg ggacatagcc
     1921 gatgatcgga cggatcgcct cggtgtcggt gagggggatc ccgaagatct ccgcggatcc
     1981 cgaggtcgga cgaagcagcg tcgcgaggat cgacatgagc gtcgacttgc cggcgccgtt
     2041 cggcccgatg agcccacaga ccgtcccgga ttcgatctgg acgctcaacg agtcgagcgc
     2101 acggttcgtg ccgtaggtct tcgtgagcga ctcggtgcgg atggcgatgc cggagcgtcc
     2161 ggcttcagcg ggggaaatcg gcgcttcggt catggctgca ccccgcacaa gatgtgattc
     2221 gaactcgact gaatctgaaa ctcacccgca cggaatcgga tgatgcgttg ccagcccggt
     2281 tcggccgctc ccaggtcgta ggtgcgcaca ccgatcgggt cgacgtagcg ctccgacagg
     2341 ccctccaccc cgaagggtat tgccgcccag tgcgggttct cctcgtccca gatggcgacg
     2401 tcgtcgacga agtcggcgat cgagcaacgc gccgtggcga ccgacgcgtt cgcggtgatg
     2461 cgataggcgc tccagccgtc ccgggtgccg ccgaggggct ccacgatgaa cgctgcgtgg
     2521 gcggcgtcgg ggtcgaccgg cccgctccag ccgctcaccg ttgcagtcgc ggccacgaca
     2581 tcgagtcgcg aacggtcctg gccaaaggtg tcgtcgtcat cggccaggta cccggcgact
     2641 cgtacgactc cgttcgtgac catgttgtcg atgatgggga acccgccgaa attcacgttg
     2701 gagtcgcaca tcatggcgca gtcgctcgtc gcaagccccc agccgaaggg ctttgcccag
     2761 gcatcgatgt cctgggcggt ttcgaaatcg agggtgaccg tttcgccggg gcggacacgg
     2821 tcgaacgatc gactgcgtcc gaaacccgac acctccgcgg cgaccaacgg agaatcggtg
     2881 tggttggtga cggcgacgtg aaccagtgtg ccctccgcag gggtgacgac cagggtgatc
     2941 ggggaggtct cgtccaactc gaagctgccg gcgacgaggg gttcccaacc ggtgccctcg
     3001 ccccgggtgg tgaccttgtc gccgtccacc accaccgagt tcgatgaccc ggtgacgttc
     3061 cacccctgcg gcagacgggt ctgcgcagtc ccggcggtgg ccgtgatcgc cgcgtgggtc
     3121 gacccgagcg gcgagacgct ttggcgccca tagatcacgg gtcgcgccac gtcgaggttg
     3181 ccnnnnnnnn nncatcacca ccaggccgcc gatcgcgagg gccaacaacg gcgcggcgaa
     3241 ccacatgaac tggggtcggc ctgtcttgcg cagcacgaac cacagcgctg ggccgatgag
     3301 gagcatcgaa agtgcgagca gtccgagcag cgtcgaggtg gaggctcgtt cggtctcgat
     3361 gagtccgcgc cagctgaggt cgttgccgac gtcgccgaac cattggtcct ccggcccgat
     3421 cgccgtgccg gtgtcgagct gtggctgtac gacgttgcgc caatcgcccc gcaccgcggc
     3481 gtcgccgacg aagcggatct gcactgcgcc tcgttgggcc tcggtggcgt cgatcggttg
     3541 gtcgaacacg gggtcgtgag cgggagcgac cccgtcgatc acgaggcggc cacccgccca
     3601 cacccagtgc ccgatccgcc gcttgagacc gtcgtcgaag ccgtcgagtt ctgccgtgga
     3661 cacgacgagt tccccgaggt gttcgagcgc cgccggtcgt ttgagccact ctgcgtccac
     3721 ccggtcgatg gtggcgctga aggtgttgtc gaacaggtcg atcgtctgag cggtgtcggg
     3781 agcgaggttc gaagtgactc cgacgaggct gcccgaggcc ttcatgggct gtagcggtac
     3841 ccgggcgatt cggcgcccgt cctcgcgtac gacgatcgct gtcggcaggc cgatcgtgag
     3901 cccgacgtgg tcgatgaacg cagtcgcatc acggttcaga tcgagtcgat aggcggtgtc
     3961 gccgacgttg gactcgacga ccacctcgac ctggcgtgag cggtcggagg tgacctcgat
     4021 gcggaccggc agcggattgt tggtgccgta gcggttgttc aacccgacgg tcgccgccac
     4081 gtcgataccc gcgtcggcaa ccgcgtcggc agggttcggc tgcccacggt cctcagaccc
     4141 ggcggggctg atcggtacgg tcgtctcggc gggctgtggc tcgagttgtc gttgcccgga
     4201 gagcgacgct gaggccggca cggccgttat cgacgtggtc gagatagcgg ccgcgaaaca
     4261 cgccgcggcg ataatgagga gtttgggccg acccatatgg agggcgacca taacagtctg
     4321 tggccgtcct ccatatgtcg gttgtgtgag aattcggcga acctgaaatc cgtcagctct
     4381 cgggccagcg gaagtcggcg aaacgatcgc gaaggtcttt cttcgagaac ttcccgaccg
     4441 aggtcttggg cacctcgtcg atgaactcca cgccgtcggg aagccaccac ttggccaccc
     4501 tcggagcgag ccattccagc agctccgcct cggtgagctc cgacccttcg gccaacacga
     4561 cacaggccag gggtcgctcg tcccatttcg ggtgggcgat gccgatcaca gcggcctcca
     4621 cgacatcggg gtgagccatg atctcgtttt cgaggtcgac cgaactgatc cattcaccgc
     4681 cggatttcac caggtccttc gtgcggtcga cgaggcggat gtagccctcg gaatccaccg
     4741 tcgccacgtc gccggtgcgc agccaaccgt cggtggtgaa cgactccgcc gagcgcaggt
     4801 cgttgtagta ggtgccggtc acccacggcc cgcggacctg cagctcgccg cggctctcgc
     4861 cgtcccacgg caactcgtcg atggtgttcg gttcggtcac ccggaactcg accccgggta
     4921 cgacgagacc gacggtggcc cggagttccg gggtgtcctc caggtaggac ttgatcaacg
     4981 tcgcggccgc caccggcgaa gtttcggtca tgccccaggc ctggaagatc ggcaggccga
     5041 tcttggcccg gaacccctcc gacaacgccg gcggcaccgc ggagccgccg cacgggatga
     5101 gccgcaacga actgacgtcg tgtccgtcga tggcgttgag cacacccatc cagatcgtgg
     5161 gaacaccggc agcgagcgtg acgcggtagt cctccatcag cttcacgagg ctcggcgggg
     5221 tcagatccgg gccgggcatg atgagcgtcg cacccgtggc gacggcggcg tgagcgaggc
     5281 cccaggcgtt ggcatgaaac atcggcacca cgggcaacac gacgtcggac tctcgcatcc
     5341 cgaccacgtc ggccatcatc gcggcaaagg tgtgcagcac gatcgagcgg tgggtgtaga
     5401 cgacgccctt cgggttgccg gtggtgccgg aggtgtacat catcgcggcc gcagtgttct
     5461 cgtcggtgat gttccactgc atcggggact cgttcgcgat gagttcctcg tagtcgtgca
     5521 cgacgatgcc gttgggtgcc tcggttggaa tcggtgcgcc cgtgtcgttc atgacgacga
     5581 cgtgtttgac ggtcttgaaa tgttcgagtg cgggccaggc caacggagcg atcgagttgt
     5641 cgatgaagag cacctcgtcc tcggcgtgat tcacgatgaa cagcagctgt tcggggaaca
     5701 gccgaaggtt gatcgtgtgg cacacccggc cggaacacgg gaccgcgaag tacaactcga
     5761 ggtggcgggc ggtgttccag ccgaaggtgc ccacccgtcc gccgggtgag atcccgaggg
     5821 tgtcgagcgc cgagccgagg cggcggctgc ggtcggccca ctccccatag gtggtccgct
     5881 cgatgccctt cggagagctg gtgatgatct cctttttcgc aaagagctgt tctgcgcgct
     5941 ggaacaggtg cggcagggcc agcgggaaat ccatcatcgt gccgagcatg gcaacctcct
     6001 ggtcgtcctc gaagcccccc tttggacaac gagtgctcac gaaattacag cgacgcgggc
     6061 ggtcgcgctg cgggaccatg ggtccatggc cgaaaccccg ccgcccacgc caccgccgac
     6121 ctcaccgacg accgcagcct cgaccgactc gacgccgccg actccagagg ttggatccaa
     6181 ggtgatctcg cccacgccgg cgacgaccat ccgcaacgcc aagcgggcgc gactggaccg
     6241 cgaggaggcc tacgcgatcc tcgacgacga cgtggtggcc cacgtcgggt ttgtgctcga
     6301 cgctcagccc ttcgtcattc cgatggtgta cgggcgtcgg ggagactcgt tgttcctgca
     6361 cgggtcggtg gccacccgca tcatgagggc cctcgacgcc ggggtcagcg catgcgtgac
     6421 cgtaacgaag ctcgacgggc tggtgctcgc ccgcagccac ttccaccact cggtcaacta
     6481 ccgctgtgtg gtggccctcg gcaccgcacg ccggctgcgt gacgacgcgg cgatggacgc
     6541 cttcgacgtc atcatggagc acctgatgcc gggtcgtgtc gaggaggccc ggcgggcgaa
     6601 ccgccccgaa caacgccaga cgatggtgat cgagttcccc ctcgtcgaat tgtcggtgaa
     6661 ggttcgcgac ggcgggccgg tcgacgacga agtcgacctt ccggaccccg acgagttgag
     6721 cgacctctgg gcaggggtgg tcccgttgcg agtcgtcgcc gggatcccga tcgcggatga
     6781 gttcacctcg ccgaacgcac cgttgccctc cgccgtggcc accggggcga cccgatggcc
     6841 agccgggtga gcgaggcgcg cccgtgccgc cggttcgacg ccggcgccaa acgcgaggac
     6901 tgggcgcgct agcgggtcgg atgtgacccg ctagcgcgcc cagtcctcat cgaaccggtc
     6961 aggtgtgggg cggcgtcagg cgctctgaag cgacttggag tcgcgcagca cctgttcggc
     7021 gaagtcgatc gcgtcgctgt cgaggtggcg gcggcccaat tcgaggcccc caagcacctt
     7081 cgcgcccttc gactcgagga ggcgccggaa cccgcgcaac acgttgccgg gccgcaccgc
     7141 ataggtgacg aaggtgtaca ccggcttgtc ccagatctcg ggaaggtgcc gtgcgatgtt
     7201 gccggccccg ccgggacggt ggccggcgat gatgaggccg tcggtccagg tgccgatgac
     7261 gacgagatcc gactcggcga gggcttcgag g
//
LOCUS       DAOOMA010000048         7229 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_12230_length_7229_cov_3.768051, whole genome shotgun sequence.
ACCESSION   DAOOMA010000048 DAOOMA010000000
VERSION     DAOOMA010000048.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 7229)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7229)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7229
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12230_length_7229_cov_3.768051"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(21..893)
                     /locus_tag="PLP95_02380"
     CDS             complement(21..893)
                     /locus_tag="PLP95_02380"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HPB44676.1"
                     /translation="MKFGLSLPYGAGVAGDPEWMRSFVADAEASGFESVYLVEHIVMV
                     DGHRDVYPYAESGRVPLRNDCAIPDPIDVLCYLAAVTDRIRLGTSVLVAPHHHPLMLA
                     KRLATLDLYSSGRAMVGLGVGWMREEIEACGGRFDCRGRQTDELIDAMRAAWNESPAN
                     FHGEYFSFEGAMSYPKPLQPGGVPIHIGGHSMAAARRAGLRGDGFQPLGVTGDDLARR
                     MVEMRRSAESVGRDPDAIELTLRGSLASIDEAAVEAIRAAGAHRVILSCTTAELERLA
                     EQMWAVSALMDVIG"
     gene            974..2077
                     /locus_tag="PLP95_02385"
     CDS             974..2077
                     /locus_tag="PLP95_02385"
                     /inference="COORDINATES: protein motif:HMM:NF021599.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2332 domain-containing protein"
                     /protein_id="HPB44677.1"
                     /translation="MDLARLEGWFRRFADIECRDLPMYHRLCHGVADHSDLLERLLIA
                     APGQWRPNLMLAALHDIVLWDPEVPLARWYPTVGGAPDAAGDLIDAVRSTIETHPNRF
                     AELIATRSTQTNEVNRSTLWPLALAAATADVDAEVALLEIGPSAGLNLRFDEYDIRYV
                     DSSGGTVGRFGTRSSPVHLRCEVRGDAPTVRHHVESTATVPPIVARIGIDAAPVDLGD
                     PDQRRWLKACIWPEQIERHERFDAAASHMVRRQAIDPLTLIRDDALDGLFDAVERFPA
                     EAHLVVINSWMMTYLHRDRRSAFDRALDLLGDTRTLTHISAEGEGVESWVPAGDGPTD
                     DTVVGISRWRQGVRSDQRWALCHPHLTWFAPLD"
     gene            complement(2106..3299)
                     /locus_tag="PLP95_02390"
     CDS             complement(2106..3299)
                     /locus_tag="PLP95_02390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA acetyltransferase"
                     /protein_id="HPB44678.1"
                     /translation="MSSHGIKDRVAIVGMGCTPFKEHWDKGADDLLIDAAQETFASAG
                     MAKDDVDAFWFGTAQSAMSGVPLASALKLDNKPVSRVENFCATGSEALRQACYAVASG
                     AYDTAMAIGVEKVKDSGYQGLNAFPIPNDGTARTLTAAAMFSLVLPAYAEKYGVDRDE
                     LRRTVARIASKNHFNGARNPKAMYRREMEVDAICKMAAVAGQLSVMDCAGVADGSAAA
                     IVVRAEDAHKYTDKPLYVKALSFVAGNGSGLIDSTYDYTTFRECEAAANDAYQQAGIT
                     DPLNQLAMAEVHDCFTPTEMVLMEDLGFSKRGEAWQDVNAGVFDLGGQLPVNPDGGLK
                     SFGHPVGASGLRMIYEAWLQLRGEAPEERRIATADRGLALTHNLGGYPGEMVSFVGIY
                     GSQLG"
     gene            complement(3296..4699)
                     /locus_tag="PLP95_02395"
     CDS             complement(3296..4699)
                     /locus_tag="PLP95_02395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020379015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold domain-containing protein"
                     /protein_id="HPB44679.1"
                     /translation="MRGIISAAAYVPYWRLERSAIAAFHGGRGAGVRSVASYDEDTTT
                     LAVEAGRLALRSRVVGTDVSPETLWFATSAPTYAEKTNASVVHAALRLPTSVAAFDAT
                     GALRSGIGVLRSAVRSSDTVLATAADIRTGPANSPDESAGGDAGAAVLVGDDSAGAVI
                     AEYLGGASSTHEFLDRWRAPGEVRTKAWEERFSETQYLNRGLEAFNAALDSTGLDADG
                     VDAVLVAGPHTRANNALAKRLGKANAVDHSSTIGNAGSAEAMVQLVEWLEGATPGQTV
                     ALISLADGADVLIFRTTDAIGSYTPARSVASQIEAGNTSLPYNKFLAWRGTLVPNPPN
                     RPEPARSSGSAAGRSYDWKYGFVGSKDRESGAVHLPPARVSFEGGNVDDMEPWPMADA
                     AGTIVTFTVDRLVYSPSPPVIFAVVDFDGGGRLPVEVCDVTQDAIAVGDRVDMTFRRI
                     NSADGISNYFWKARPIR"
     gene            4793..5524
                     /locus_tag="PLP95_02400"
     CDS             4793..5524
                     /locus_tag="PLP95_02400"
                     /inference="COORDINATES: protein motif:HMM:NF014625.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HPB44680.1"
                     /translation="MATSTPALFDVVPENWERALAIVAHPDDLEYGAASAIARWASQG
                     KHITYLLVTCGEAGIDSMPPDECARVRMQEEIDSAAVVGVERVEFLGHPDGLVEASLA
                     LRRDLVGAIRRHRPDVLIGLNFRETFPFGGFNHADHRATGWAILDAARDAANRWLFVG
                     HGGAPHAARFALFANSPQANTYVDIGDTVHLGVESLRAHGIYLAALADGTAGKDPEPF
                     IRGMATAAGEAVGVDSAVLFERIDL"
     gene            complement(5576..6220)
                     /locus_tag="PLP95_02405"
     CDS             complement(5576..6220)
                     /locus_tag="PLP95_02405"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HPB44681.1"
                     /translation="MGSPISGIARWYEQRVLPRFVERACNAPGIRRLRPKATAGLVGT
                     VIEVGFGAGPNVEFYPPDVTRVLAVEPSELSRDRAAARVAASSVDVEFVGLDGARVPV
                     DDDVADAGLVTFALCTIPDVAAALGELRRILKPGAKLHVLEHGLAPDESVVRWQRRLN
                     PIQKRLGGGCHLDRDPVAMLSDAGFIDIDASQRYLNSTPKFIGYTSLITAANPA"
     gene            6286..>7229
                     /locus_tag="PLP95_02410"
     CDS             6286..>7229
                     /locus_tag="PLP95_02410"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF014564.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HPB44682.1"
                     /translation="MRVGDIANEEALPFGKPLEGVRILAAEQMQALPWATQLLARLGA
                     EVVKVEHPVHGESGRASLPGMTDPEGRNVGATFLRNNLNKRSVGLDLKHPKGRELFIE
                     MAGQFDIVAENFKAGTMQRLGLGYDTLSKIHPSLIYVSVSGFGNTGESPYQDWPAYAP
                     VAEAMSGIYEYSRREGEPPRILPVGALGDIGTGLFATIGILAALQHRQRTGVGQYVDI
                     AMLDSLVSITDVVTTIWAMGMRPNSLSGIMNAFKASDGYFAVQVVREHQFENLAKLIG
                     AEWWLTDERLADRSLWFQNTDSVIRPTFEQWASHYTRL"
BASE COUNT         1230 a         2497 c         2337 g         1165 t
ORIGIN      
        1 cctctcgtcg tggcgaaacc tcagccgatg acgtccatca aggccgagac cgcccacatc
       61 tgttcggcga ggcgttcgag ttccgcggtc gtgcacgaca ggatgacacg gtgagcccca
      121 gcggcccgaa tcgcctcgac ggccgcctcg tcgatcgatg cgagcgagcc ccgcagcgtc
      181 aactcgatgg cgtcgggatc gcggccgacc gactcggcac tgcggcgcat ctcgaccatg
      241 cgtcgtgcga ggtcgtcgcc ggtcacaccg aggggctgaa acccgtctcc gcgaagaccg
      301 gcccgacgtg ccgccgccat cgaatgccca ccgatgtgaa tcggcacccc gcccggctga
      361 aggggtttgg ggtacgacat ggcaccctcg aagctgaagt actcaccgtg gaaattcgcc
      421 ggggactcgt tccacgcagc ccgcatcgcg tcgatcagct cgtcggtttg ccgaccacga
      481 cagtcgaaac gaccgccgca tgcctcgatc tcctcgcgca tccagccgac gccgaggcca
      541 accatcgcac gccccgatga gtagaggtcg agggtggcga ggcgcttcgc cagcatcagt
      601 gggtggtggt gcggagccac cagcacgctc gtaccgagac ggatgcgatc ggtcaccgcc
      661 gcgaggtaac acagcacatc gatcggatcg gggatcgcac agtcgttgcg aagcggcacc
      721 cgccccgatt ccgcgtaggg gtagacgtcg cggtgtccgt cgaccatcac gatgtgttcg
      781 acgaggtata ccgactcgaa cccgctcgcc tcggcatcgg ccacgaacga ccgcatccat
      841 tccggatcac cggcgacgcc tgcgccgtac ggcaaactca aaccgaactt catggattcg
      901 acgctacagc cgacgacggt gtcgaatcgg tcggggcagg gatgctgcgg gcaacctgtg
      961 ccatcgttga cggatggacc tcgcccgtct cgaaggctgg tttcggcgct tcgccgacat
     1021 cgagtgccgc gacctgccga tgtatcaccg actgtgccac ggcgtcgccg accatagcga
     1081 cctgctggag cgcctgctca tcgccgcacc cggtcaatgg cgaccgaacc tgatgctggc
     1141 ggccctgcac gacatcgtgt tgtgggaccc agaggtgccg cttgctcgct ggtaccccac
     1201 ggtcggtggc gcacctgacg ccgctggcga tctcatcgac gccgtccgct cgacgatcga
     1261 gacccacccg aatcggttcg ccgaactcat cgccacccgc tcgacccaga ccaacgaggt
     1321 caaccggtcg accctgtggc cgttggccct cgcggcagcg accgccgatg tggatgccga
     1381 ggttgcgctg ctggagatcg gcccctcggc ggggctgaac ctgcgattcg acgaatatga
     1441 cattcgctat gtcgattcat caggtgggac ggtcgggaga ttcggcaccc ggtcatcgcc
     1501 agtgcatctg cgctgcgagg tgcggggcga cgctccgaca gtgcgccatc acgtcgaatc
     1561 cacggcgacc gtccccccga tcgtcgcccg catcggcatc gatgccgcgc cggtcgacct
     1621 gggagacccc gaccaacgtc gatggctcaa ggcctgcatc tggcccgagc agatcgaacg
     1681 ccatgaacga ttcgatgccg cggccagcca catggttcgc cgccaggcca tcgaccctct
     1741 caccctgatc cgtgatgacg cgctcgacgg cttattcgac gcggtcgagc ggttccccgc
     1801 tgaggcccac ctcgtggtga tcaacagctg gatgatgacc tacctgcacc gcgaccggcg
     1861 cagtgccttc gaccgagcac tcgacctact gggcgacacc cgcaccctga cccacatcag
     1921 cgccgagggc gagggggtcg agtcatgggt gccggcgggc gacggcccga ccgacgacac
     1981 cgtcgtgggc atctcgcggt ggcgccaggg tgtgcgcagc gaccagcgct gggcgctgtg
     2041 ccatccgcac ctgacctggt tcgcgccgct cgattgagcg gcacgaaccg atgtcgtgat
     2101 cagaatcagc cgagttggga tccgtagatt ccgacgaagc tcaccatctc gcccgggtat
     2161 ccaccgaggt tgtgggtgag ggcgaggccg cgatccgcgg tggcgatgcg ccgctcctcg
     2221 ggggcttcgc cacgcagctg caaccaggct tcgtagatca tgcgcagacc ggacgctccg
     2281 accgggtggc cgaagctctt caggccccca tcggggttca ccggcaactg gccgccaagg
     2341 tcgaacaccc cggcgttgac gtcctgccat gcctcgccgc gcttggagaa accaaggtcc
     2401 tccatcagca ccatctcggt cggggtgaag cagtcgtgca cctcggccat ggcgagctgg
     2461 ttgagcggat cggtgatacc ggcctgttgg taggcatcat tggccgccgc ctcgcactca
     2521 cggaaggtcg tgtagtcata ggtcgagtcg atgaggcccg aaccgttgcc ggccacgaac
     2581 gacagcgctt tcacatacag cggcttgtcg gtgtacttgt gcgcatcctc ggcgcgcacg
     2641 acgatggctg cggccgaacc gtcggccacg cccgcgcagt ccatgaccga gagctgaccg
     2701 gcaaccgccg ccatcttgca gattgcgtca acctccatct cgcggcgata catcgccttg
     2761 gggttgcggg caccgttgaa gtggttcttc gacgcgatcc gggccacggt gcggcgcaac
     2821 tcgtcgcggt cgacgccgta tttctctgcg tacgccggca gcaccaggct gaacatcgcc
     2881 gccgcggtca gcgtgcgggc ggtgccgtcg ttcgggatcg ggaacgcgtt gagcccctga
     2941 tagccagagt ccttcacctt ctcgacgccg atggccatgg cggtgtcata cgcacccgag
     3001 gcaacggcgt aacacgcctg gcgcagagcc tcggatccgg tggcgcagaa attctcgacg
     3061 cggctcaccg gcttgttatc gagcttgaga gccgaggcga gcggaacgcc cgacatcgcc
     3121 gactgagcgg tcccgaacca gaacgcgtcg acgtcgtcct tggccattcc ggccgacgcg
     3181 aaggtctcct gggcggcgtc gatcaacagg tcgtcggcgc ctttgtccca atgctccttg
     3241 aacggggtgc atcccatccc gacgatggcg acgcggtcct tgatgccatg gctgctcatc
     3301 ggatgggcct cgctttccag aagtagttgg agatgccgtc cgcgctgttg atgcggcgga
     3361 aggtcatgtc gacccggtcg cccacggcga tcgcgtcctg ggtgacgtcg cagacctcga
     3421 cgggcagacg cccgccaccg tcgaagtcga cgacggcgaa gatgaccggc ggactcggcg
     3481 agtagacgag acggtcgacg gtgaaggtca cgatcgtgcc ggcggcgtcc gccatcggcc
     3541 acggttccat gtcgtcgacg ttgccgccct cgaagctcac gcgcgccggc gggaggtgca
     3601 cggcgccgga ctcgcggtcc ttcgacccga cgaatccgta tttccagtcg tagctgcgcc
     3661 cggcggccga gccggaggag cgggcgggtt ccgggcgatt gggcgggttc ggcacgagcg
     3721 tgccgcgcca ggcgaggaac ttgttgtacg gcagcgaagt gttgccggcc tcgatctgcg
     3781 atgccaccga acgtgcgggg gtgtaggagc cgatggcatc ggtggtgcgg aagatcaaca
     3841 cgtcagcgcc gtcggcgagc gaaatgagcg caaccgtctg gccgggggtc gccccctcga
     3901 gccactcgac gagttggacc atggcctcgg ccgatccggc gttaccgatg gtggaggaat
     3961 ggtcgacggc gttcgccttg ccaagcctct tcgccagcgc gttgttggcg cgggtgtgcg
     4021 gaccggcgac gagcaccgca tcgaccccgt cggcgtcgag cccggtggag tcgagcgccg
     4081 cgttgaaggc ctcgaggccg cggttcaggt attgagtctc gctgaagcgc tcttcccagg
     4141 ccttggtgcg cacctcgccc ggggcgcgcc agcggtcgag gaactcatgg gtgctcgacg
     4201 cgccgccaag gtactcggcg atgaccgcac ccgcactgtc gtcgcccacc aaaacggccg
     4261 cgccggcgtc accaccggcc gactcgtcgg gcgagttggc cgggccggtc cggatgtccg
     4321 cggccgtcgc caggaccgtg tcggagcttc gcaccgcgct acgcaggacc ccgatgcccg
     4381 aacgcagcgc gccggtggca tcgaaggccg cgaccgaggt cggtaaccgc agcgcggcgt
     4441 gcacgaccga agcgttggtc ttttcggcat acgtcggcgc cgaggtggcg aaccacaggg
     4501 tctccggtga cacatcggtt ccgacgaccc gacttcgcag cgccaatcgg cccgcctcaa
     4561 ccgccagggt cgtggtgtct tcgtcgtagg aggcgacact gcgaacgccg gcgccacgac
     4621 caccgtggaa cgccgcgatc gccgatcgct cgaggcgcca gtacggcaca tatgccgcgg
     4681 cgctgatgat tcctcgcatg ccgcaaagtg tacgccgttt ctgacgatcc gtcagaaacg
     4741 gccggtggga gaccgtccgt cccggcaccg agccgcgacg tagcctcggg gtatggccac
     4801 ctcaacaccc gccttgttcg acgtcgttcc cgaaaactgg gagcgggccc tcgccatcgt
     4861 ggcccatccc gacgacctcg aatacggcgc tgcctccgcc atcgcccggt gggcctcaca
     4921 gggcaagcac atcacctacc tgttggtgac ctgcggcgag gcggggatcg actcgatgcc
     4981 gcccgatgaa tgcgcgcggg tgcgcatgca ggaggagatc gattccgccg cagtggtcgg
     5041 cgtggaacgg gtcgagttcc tcggccaccc cgacggcctg gtcgaggcat cgctggcgct
     5101 gcgacgcgat ctggtcgggg caattcggcg acaccgaccc gacgtgttga tcgggctgaa
     5161 cttccgcgag accttcccgt tcggcgggtt caaccacgcc gaccaccgag ccaccggttg
     5221 ggcgatcctc gacgcagcac gcgatgcggc gaatcgctgg ctcttcgtcg gccacggcgg
     5281 cgccccacac gccgctcgct tcgcgctgtt cgccaactcc ccacaggcaa acacctacgt
     5341 cgacatcggc gacaccgtgc acctcggggt cgagtcactt cgggcgcacg gaatctatct
     5401 tgcagccttg gccgacggca ccgccggcaa ggatcccgag ccgttcatcc gcgggatggc
     5461 caccgcggcc ggcgaggccg tcggcgtcga ttcagcggtg ttgttcgaac gcatcgacct
     5521 gtagaccggc acctcaggcc gggttcgtca ggccgggttc gtcaggccgg gttcgtcagg
     5581 ccgggttcgc cgcagtgatc aacgaggtgt agccgatgaa tttcggggtg ctgttgaggt
     5641 agcgctgcga ggcgtcgatg tcgatgaacc cggcgtcgct cagcatcgcc actggatcac
     5701 gatcgaggtg acatccacct ccgagacgct tctggatcgg gttgagacgc cgctgccaac
     5761 gcacgaccga ctcatccgga gcgaggccat gttccaacac gtgcagcttc gcccccggct
     5821 tcaagatccg gcgcagttcg cccaaggcgg ccgccacatc aggaatcgtg cacagcgcga
     5881 atgtcaccag tccagcgtcc gccacatcgt catcgacggg aacgcgggcc ccatcgagac
     5941 cgacgaattc gacatcgacc gatgaggcgg ccacccgcgc tgcagcccga tcgcggctca
     6001 gttccgatgg ttcaaccgcc agcacccgcg tgacgtccgg cggatagaac tcgacgttgg
     6061 gccccgcgcc aaacccgact tcgatgaccg tgccgaccaa tccggcggtg gccttcgggc
     6121 gtaggcgacg aatcccggga gcgttgcacg cccgctcgac aaaacgcggc aacacccgct
     6181 gctcgtacca ccgcgcaatg cccgatatcg gcgaacccat gctgcgacgc taccaatccg
     6241 agccgtcctc tgacgatcca tcaggttcgc gcctacgctt cgcccatgcg cgtcggagac
     6301 atcgccaacg aagaagccct cccgttcgga aagcccctcg aaggcgtccg aattctcgcc
     6361 gccgaacaga tgcaggcgct gccctgggcg acccagctcc tggcgcggct cggtgcggag
     6421 gtcgtcaagg ttgagcaccc ggtccacggc gagtcgggcc gggcctcgct gccgggcatg
     6481 accgaccccg agggccgcaa cgtgggcgct accttcctgc gcaacaacct caacaagcga
     6541 agcgtcggac tcgacctcaa gcaccccaaa ggccgcgagc tgttcatcga gatggccgga
     6601 cagttcgaca tcgtcgccga gaacttcaag gccggcacga tgcagcgcct cggcttgggc
     6661 tacgacaccc tgtcgaaaat tcacccctcg ctcatctacg tctcggtgtc gggattcggc
     6721 aacaccggcg agtcgccgta tcaggactgg ccggcgtacg cccctgtggc cgaagccatg
     6781 tcgggtatct acgagtactc acgccgcgag ggcgaaccgc cacggatcct gcccgtcggc
     6841 gcactcggcg acatcggcac cggcctcttc gccacgatcg gcatcctcgc cgcgctgcaa
     6901 caccgccaac gcaccggggt cggccagtac gtggacattg cgatgctcga ctcgctggtc
     6961 tcgatcaccg acgtcgtcac gacgatctgg gcgatgggca tgcggccgaa ttcgctcagc
     7021 ggcatcatga acgccttcaa ggcctccgac ggctatttcg cggtgcaggt cgtgcgcgag
     7081 caccagttcg agaacctggc gaaactcatc ggcgccgagt ggtggctgac cgacgaacgc
     7141 ctcgcggatc gctcgctctg gttccagaac accgactcgg tcattcggcc gacgttcgag
     7201 caatgggcgt cgcactacac ccgcctcga
//
LOCUS       DAOOMA010000049         7202 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_12314_length_7202_cov_4.150413, whole genome shotgun sequence.
ACCESSION   DAOOMA010000049 DAOOMA010000000
VERSION     DAOOMA010000049.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 7202)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7202)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7202
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12314_length_7202_cov_4.150413"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(18..242)
                     /locus_tag="PLP95_02415"
     CDS             complement(18..242)
                     /locus_tag="PLP95_02415"
                     /inference="COORDINATES: protein motif:HMM:NF012885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB44683.1"
                     /translation="MSPTPVTPKPMPFEKYDAFVPLALPDRTWPDRSLTKAPRWCAVD
                     LRDGNQALIDPMDPARKLRMFQTLVDMGFK"
     gene            480..1961
                     /locus_tag="PLP95_02420"
     CDS             480..1961
                     /locus_tag="PLP95_02420"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HPB44684.1"
                     /translation="MLLHEAVEFAARYDGDAVALITEDGVLTYADLWAEVVRIGAWVA
                     SRVAPGERIAVVSDNRAEVVALMYGAPMVGVTVMFANTRLVSAEIDALIDDVDPQLVV
                     GESRLLDGLKAGRATVAFDSPEFGRLDGAAGVSSRPVDVGDPAWIIHTSGTTGRPKGA
                     VLTHRSLMAGVLATALARPLGDGDSYLFPFPLFHVAAYNVIHAHLKRRPVVLVSKFDA
                     EVVMTLIEAHRVTTLSLAPTMISMLLDHPSLRSYDLSSLRHIFYGASAIPLDVLRRAV
                     AVFGCGFGQGYGMTELSGNAVFLDAQAHARAIDGEEHLLAACGKPSPLVSIRLDDAGE
                     ILVRGDQVIDGYWNRPDADDDAYADGWFRTGDIGRFDEEGYLYIVDRKKDIIVSGGEN
                     VASLEVEDVMSEHPAIGAVAVVGVPDERWGERVVAVVVERDGHQLDEAEAVAWAKGRL
                     AGYKRPKQVLVVEALPMNASGKVLKREVRDLATRRLARPNSPT"
     gene            complement(2047..3369)
                     /locus_tag="PLP95_02425"
     CDS             complement(2047..3369)
                     /locus_tag="PLP95_02425"
                     /EC_number="2.6.1.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004015 -
                     adenosylmethionine-8-amino-7-oxononanoate transaminase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine--8-amino-7-oxononanoate
                     transaminase"
                     /protein_id="HPB44685.1"
                     /translation="MAAERSADQLLALDQAHVWHPYAAMPATVPAALVNSASGVRLRI
                     ASPQAGSDPASTSEVIDAMSSWWAACWGYRHPVLDAAAHAQIDSMSHVMFGGLTHRPA
                     IELVERLVALAPAGIEHVFLADSGSVAVEVATKMALQYWHGLGQPLKRRLLTWRGGYH
                     GDTLHPMSVCDPDGGMHATWTGTLPEQVFADAPPPGFAAPLEETYVAHLRSVIESHLK
                     ELAAIIIEPVVQGAGGMTFHSPEYLRVWRGLADEYDLLLIFDEIATGFGRTGEIFAAD
                     HAGVTPDIMCVGKAMTGGYLTMAAAMCTPRVSATIAQGDLPVLAHGPTFMGNPLAAAV
                     ANASIGLLDSTPWRTWVPRIERELVEGLEGLRDHPAVADVRVLGAIGVCELREPVDMA
                     AATRAALDSGVWLRPFGRLVYTMPPYISTTEEVAAICAAIAAVADTNR"
     gene            complement(3372..4418)
                     /gene="bioB"
                     /locus_tag="PLP95_02430"
     CDS             complement(3372..4418)
                     /gene="bioB"
                     /locus_tag="PLP95_02430"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00433.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004076 - biotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HPB44686.1"
                     /translation="MSNTAETPTQTASIESAPDELIQAARVRLIDRGGHLSLDEVRAL
                     SGLPTSKLGELMELAHEVRLARCGDMVEVEGILSVKTGGCPEDCHFCSQSSKFDTPVA
                     ATPFLDTEEVLEAARETAALGASEFCIVLAVRGPDEKTLQRILELVPIIGDETGLQVA
                     VSAGILTEDQARRLAEGGVHRYNHNLETSRSYFPNVVTTHDFDERWETCELVKHNNME
                     LCCGVLLGMGETDEQRLELIGHLQDLQPTEVPVNFLNPRPGTPLELRPITPAKDALKW
                     VALFRLALPEVILRYAGGREITLGDMQEAGMTAGINALIVGNYLTTLGRTPDQDLDML
                     DRLSMPVGVLTKVV"
     gene            4590..5744
                     /locus_tag="PLP95_02435"
     CDS             4590..5744
                     /locus_tag="PLP95_02435"
                     /EC_number="2.3.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008710 - 8-amino-7-oxononanoate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="8-amino-7-oxononanoate synthase"
                     /protein_id="HPB44687.1"
                     /translation="MAREAAEQLRAQDRWRVIRTLADTGVRSRLADGRAVVQFASNDY
                     FGLSEHPAVIEAAIAATRRWGTGAGASRLVVGSRPIHDDLEAALADWKHAEAALLFAT
                     GYAANVGVLVALARLGAALGAPIGIVSDELNHASIIDGIRLSAAPFNIYAHADAEAAR
                     QRVVEHLDAGRRPVIVTDSVFSMDGDLAPLGDLAQLAAETSALLVIDDAHAVFAGCGA
                     DLVASAPSRGGAEVVVVGTLSKSLGSLGGFVAGPTALVDWCRNTSRSFIFSTATPPGV
                     AAAALAALEVLQGAEGVELTAKLRRNVDVLAPAQASAVVPVVVGTAERALEWSDKLLE
                     RSMFVPAIRPPTVPEGSSRLRVAVSARHATGDLEALREALADLRRLDPTT"
     gene            5758..6627
                     /locus_tag="PLP95_02440"
     CDS             5758..6627
                     /locus_tag="PLP95_02440"
                     /inference="COORDINATES: protein motif:HMM:NF024891.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dethiobiotin synthase"
                     /protein_id="HPB44688.1"
                     /translation="MSARPRRLLAVAGASTEVGKTFVSAAVIDRLVADGWNVEVRKPA
                     QSANPGEPTDSEVLAAASGEPHERICPPNRTYSVAMAPPMAAAVLGLTAPTTDELVDE
                     VEGSWGALSADVGIVELAGGVASPMAVDGDGADLAATFRPDAVLLVADAGLGTINAVR
                     LCLARLDEALSLSSAAAGASDGVVDTPVLVFMNRYDDANDLHHRNAQWLAHQDHVVLA
                     TGLEELVDWVLSLAPRLCGYCAKAVPDDCDGACGPEFDLDRYCVRCGRRLVVSMNPVK
                     AIGRCKVHGLECA"
     gene            complement(6696..>7202)
                     /locus_tag="PLP95_02445"
     CDS             complement(6696..>7202)
                     /locus_tag="PLP95_02445"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HPB44689.1"
                     /translation="GDLHRLRSTDTGFALALCDLNEFRHYNDCHGADLGDRALRTFAE
                     VLTRTLRPSDIIARYDDDRFVCLFPSTSAQHAVAAMERVRESLVLHLALGEVAPFRAS
                     VGIVGSEEATTPEALLELAEMAIDLAKANGGNRVVVSGVTPPSVSSPSPATSPSPTAS
                     PFSDNEWS"
BASE COUNT         1121 a         2288 c         2591 g         1202 t
ORIGIN      
        1 gggaatccga cctcgatcta tttgaagccc atgtcgacca gcgtctggaa catgcgcagc
       61 ttgcgggcgg ggtccatcgg gtcgatcaag gcctgattgc cgtcgcgcag gtcgaccgcg
      121 caccaacgag gcgccttggt gagcgagcga tccggccagg tccgatccgg gagcgcgagc
      181 ggcacgaacg cgtcgtattt ctcgaacggc atgggcttcg gcgtgacggg agtgggagac
      241 atggtgtgct cgcttgctcg acgacttgat ggggtcgcgg tggatggttg ggacgacggc
      301 tcgaggatcg gtaccgaaaa agaacaaagc ccccgagccg attcggcgca ggagctttgg
      361 cgagtaccga tctgggggta ctcgcctacc ggagaagaag gagggcctgg atcgatgccg
      421 tcatcacggc cactatagcg cggcattcgg tcgatgggcg cggcggtatc gtggcaccga
      481 tgttgttgca cgaggcggtc gagttcgcgg cccgttacga cggtgacgcg gtggccctca
      541 tcaccgaaga cggcgtcctg acctacgcgg atctgtgggc cgaagtggtt cggatcggcg
      601 cctgggtggc gtcgcgggtg gcgccggggg agcggatcgc cgtcgtgtcg gacaaccggg
      661 ccgaggtggt ggcgctgatg tacggcgccc cgatggtggg ggtgacggtg atgttcgcca
      721 acacccgtct cgtatcggcc gagatcgacg cgctcatcga cgacgtcgat ccgcagttgg
      781 tcgtgggcga gtcacggttg ctcgatgggc tcaaggccgg gcgcgccacg gtcgccttcg
      841 actcgccgga attcgggcga ctcgatggcg ccgctggtgt ctcgtcgcgg ccggtcgacg
      901 tcggcgaccc ggcgtggatc attcacacct cgggcacgac ggggcgaccg aagggcgcgg
      961 tgctcacaca tcgcagtctg atggcggggg tcctggcgac ggcgttggcc cgaccactcg
     1021 gcgacggcga ctcgtatctc ttcccgttcc cgctgtttca cgtcgcggcc tacaacgtga
     1081 ttcatgcgca tctgaagcgg cgaccggtcg tgttggtgtc gaagttcgac gccgaggtgg
     1141 tcatgacgct catcgaggca caccgggtca cgaccctttc gctggcgccg acgatgatct
     1201 cgatgctgct cgaccacccg agcctgcgca gctacgacct gtcgagcctc cgccacatct
     1261 tctacggggc ctcggcgatt ccgctcgatg tgttgcgtcg ggcggtggcg gtgttcggat
     1321 gcgggttcgg tcagggctat ggcatgacgg agttgtcggg caacgcggta tttctcgacg
     1381 cgcaggcgca cgctcgagcg atcgacggcg aagagcacct gctggccgcc tgcgggaagc
     1441 cgtcaccgtt ggtgtcgatt cggctcgacg atgcggggga aatcctggtc cgcggcgatc
     1501 aggtgatcga cggctactgg aaccgaccgg acgcggacga cgacgcctat gcggacgggt
     1561 ggttccgcac cggcgacatc gggcgattcg acgaggaggg ctacctctac atcgtcgatc
     1621 gcaagaagga catcatcgtt tcggggggtg agaacgtggc gtcgctcgag gtcgaagacg
     1681 tcatgagcga acacccggcg atcggtgcgg tcgcggtggt cggggtgccg gacgaacggt
     1741 ggggtgagcg ggtggttgcg gtggtcgtgg agcgtgacgg gcaccaactc gacgaggccg
     1801 aggcggtggc gtgggcgaag gggcgactcg ccggctacaa gcggcccaag caggtcctgg
     1861 tcgtggaggc gttgccgatg aacgcgtcgg gcaaggtgct caagcgcgag gtccgcgacc
     1921 tcgcaacccg tcgcctcgcc cgccccaaca gccccacctg accgttctcc gataaccagt
     1981 ggtcgtgatc acgaccactg gttatcggag aacaggtggg cgctggtacg ggtgggtacc
     2041 ggtgggtcag cggttggtgt cggcgaccgc ggcgatcgcc gcgcagatgg ccgcgacctc
     2101 ctcggtggtc gagatgtacg gcggcatcgt gtagacgagt cggccgaacg gacgcagcca
     2161 cacgcccgag tcgagcgcgg cgcgggtggc ggcggccatg tcgaccggct cgcgcaactc
     2221 acacacgccg atcgccccca gcacccgcac gtcggccacc gccgggtggt cgcgcaaccc
     2281 ctcgagtccc tcgaccaact cgcgctcgat gcgcggcacc caggtccgcc acggcgtgga
     2341 gtcgagcaga ccgatcgacg cgttcgccac cgctgcggcg agtgggttgc ccatgaatgt
     2401 cggcccgtgg gcgagcaccg gcaggtcgcc ctgggcgatc gtggcggaca cccgcggcgt
     2461 gcacatcgcg gcggccatgg tgagatagcc gcccgtcatc gccttgccga cacacatgat
     2521 gtcgggcgtc accccggcgt gatcggcggc gaaaatctcc cccgtgcgcc cgaatccggt
     2581 ggcgatctcg tcgaagatca acaacaggtc gtactcatcc gcgagcccgc gccacacacg
     2641 gaggtactcg ggggagtgaa aggtcattcc gccggcaccc tgaacgaccg gttcgatgat
     2701 gatcgccgcc aactccttga ggtgcgattc gatcaccgaa cgcaggtggg cgacgtaggt
     2761 ctcctcgagc ggcgccgcga aacctggcgg tggggcatcc gcaaacacct gctcgggcag
     2821 ggtccccgtc cacgtcgcgt gcatgccccc gtcggggtcg cacaccgaca tcgggtgcag
     2881 cgtgtcgccg tgatatccgc cgcgccaggt gagcaggcgt cgcttcagcg gttgtccgag
     2941 cccgtgccag tactgcaatg ccatcttggt ggcgacctcg acggcgaccg atccggagtc
     3001 ggcgaggaac acgtgctcga tgccggccgg cgccagagcg acgagtcgtt cgacgagttc
     3061 gatcgccggc cggtgggtga ggccgccgaa catgacgtgg ctcatcgagt cgatctgtgc
     3121 gtgcgcggcg gcgtcgagta ccggatggcg gtagccccaa cacgcagccc accacgaact
     3181 catggcgtcg atcacctcgc tggtcgacgc cgggtccgat ccggcctggg gggaagcgat
     3241 gcgtaggcgc acgccgcttg ccgagttcac caatgcggcg ggcaccgtgg cgggcatggc
     3301 cgcgtacgga tgccagacgt gggcctggtc gagcgccagg agttggtccg ccgaccgctc
     3361 ggcggccacg ttcagacgac cttggtgagc acgccgacgg gcatcgagag ccgatcgagc
     3421 atgtcgagat cctgatccgg cgtccggccg agggtggtga ggtagttgcc gacgatgagt
     3481 gcgttgatgc ccgccgtcat gccagcctcc tgcatgtcgc cgagggtgat ctcgcgccca
     3541 cccgcgtagc gcaggatgac ctcgggaagc gccaaacgaa acagcgccac ccacttgagc
     3601 gcgtcctttg ccggagtgat cgggcgaagc tccaacggcg tgccgggccg cgggttcaag
     3661 aagttgaccg gcacctcggt cggctgcagg tcctggaggt gtccgatcag ttcgagccgt
     3721 tgctcgtcgg tttcgcccat gccgagcagc accccacagc acagttccat gttgttgtgc
     3781 ttgacgagtt cgcaggtctc ccatcgctcg tcgaaatcgt gggtcgtgac gacgttgggg
     3841 aaatacgacc gcgaggtttc gaggttgtgg ttgtagcgat gcacgccacc ctcggcgagc
     3901 cgtcgcgcct ggtcctcggt gaggatgccg gccgacacgg cgacctggag gccggtttcg
     3961 tcaccgatga tcggcacgag ctcgaggatg cgttgcaggg tcttttcgtc ggggccacgc
     4021 acggccaaca cgatgcagaa ttcgctcgcc ccgagcgctg cggtttcgcg ggccgcctcg
     4081 agcacctctt cggtgtcgag gaacggggtc gccgcgaccg gcgtgtcgaa cttcgaactc
     4141 tggctgcaga aatggcagtc ctcgggacac ccgcccgtct tcaccgacag gatcccctcg
     4201 acctcgacca tgtcgccgca tcgggcgaga cgcacctcgt gggcgagttc catcagctcg
     4261 ccgagcttgg aggtcggaag gccggacaac gcgcgaacct cgtcgaggct caggtggccc
     4321 ccgcggtcga tcagccgaac gcgcgccgcc tggatcagct cgtcgggcgc cgattcgatg
     4381 gacgcggtct gggtcggtgt ctccgcggtg ttgctcacgt cgctgtctcc gtggatgggg
     4441 gaatcgattc ttcgatatcg atgccggtac gttcgtcgcc aaggagtgcc gacgacgtcg
     4501 aaagggtacc cctctgcgcc atggaacccg aaaagcgagc acaacctcac cacccgattg
     4561 ccgcagcctc gaccagtcgg tggagcgact tggccaggga ggccgcggag cagttgcggg
     4621 cacaggatcg ctggcgggtt attcgcaccc tcgccgacac cggagtgcgc tcgaggctgg
     4681 ccgacggccg tgcggtggtg cagttcgcgt cgaatgacta cttcgggttg agcgagcatc
     4741 ccgcggtgat cgaggcggcg atagcggcga cgcgccggtg ggggaccggg gcgggggcat
     4801 cgcggctcgt cgtcgggtcg cggccgatcc acgacgacct cgaagcggcg ctcgccgatt
     4861 ggaaacacgc cgaagcggcg ttgctgttcg ctacggggta tgccgcgaat gtcggcgtgt
     4921 tggtggcgtt ggccaggctc ggcgcggcgc tgggcgcccc gatcggaatc gtgtccgacg
     4981 agttgaacca cgcctcgatc atcgacggca tccggctgag cgcagcgccg ttcaacatct
     5041 atgcgcacgc cgacgctgag gccgccaggc agcgggttgt cgagcacctc gatgccggcc
     5101 gacgcccggt gatcgtgacc gactcggtgt tttcgatgga tggggatctg gccccgctgg
     5161 gcgacctggc gcagttggcg gctgagacgt ccgccttgtt ggtgatcgat gacgcccacg
     5221 cggtgttcgc cggctgcgga gccgaccttg tcgccagcgc accgtctcgg ggcggtgccg
     5281 aggtcgtggt ggtcggcacg ctgtcgaaat cgctcgggag cctcggcggg ttcgtcgccg
     5341 ggccgaccgc gctggtcgac tggtgtcgca acacctcgcg ctcgttcatc ttcagcaccg
     5401 ccaccccacc cggggtcgcc gcagcggcgc tggcggcact cgaggtgttg cagggggccg
     5461 agggtgtcga gctgacggcc aagctgcggc gcaacgtcga cgtgttggcg ccggcgcagg
     5521 ccagcgcggt cgttccggtg gtcgtcggga cggccgaacg cgccttggag tggagcgaca
     5581 agctgctgga acggtcgatg ttcgtccccg cgattcggcc tccgaccgta cccgaggggt
     5641 catcgcggtt gcgggtggcg gtctcggctc ggcacgctac aggggacctc gaggcgctgc
     5701 gcgaagcgtt agcggacctg cgacgcctcg acccgaccac ctgagcggag attccccatg
     5761 agcgcacgcc cccgacgcct gctcgccgtc gccggcgcct cgaccgaggt cggaaagacc
     5821 tttgtctcgg ccgccgttat cgaccgcctg gtcgccgacg gatggaacgt cgaggttcga
     5881 aagccggcac aatcggccaa cccgggcgaa ccgaccgatt ccgaggtgct cgctgcggcg
     5941 agcggcgaac cccacgagcg gatctgcccg ccgaaccgca cctacagcgt cgcgatggcc
     6001 ccgccgatgg cggcggcggt gttgggcctg acggcgccga ccaccgacga gttggtcgac
     6061 gaggtcgagg ggtcgtgggg cgccctgtca gccgatgttg gcatcgtcga gctcgcggga
     6121 ggggttgcct ccccgatggc cgtggacggc gatggggccg acctggccgc gacctttcga
     6181 cccgacgccg tgctgttggt cgcggacgcc ggcctcggca cgatcaacgc cgtacgactg
     6241 tgtctggcgc gtctcgacga ggcgctctcg ctcagttccg ccgccgccgg tgcgtccgat
     6301 ggggtggtcg acactccggt gctggtcttc atgaatcgct acgacgacgc gaacgatctg
     6361 caccaccgaa atgcccagtg gctcgcgcac caggatcacg tcgtgttggc gaccggcctc
     6421 gaggaactcg tcgactgggt gttgagcctt gcgccgagat tgtgtggcta ctgcgcgaag
     6481 gcggttcccg atgattgcga cggggcctgt ggtcccgagt tcgatctcga ccggtactgc
     6541 gtgcggtgcg gccgccggct ggtggtgtcg atgaaccccg tcaaagcgat cggacgctgc
     6601 aaggtccacg gcctcgagtg cgcttgagtc cgcccgcttg attcagcgcg cccgcacgcc
     6661 cccagccacc gttctccgat aatgagtggt cgtgatcacg accactcatt atcggagaac
     6721 gggctggcgg ttggtgacgg gctggtggcg ggcgaagggc tggacaccga cggcggggtg
     6781 accccggaca cgacgacgcg gttgccgccg ttcgccttgg cgaggtcgat cgccatctcg
     6841 gcgagttcga ggagcgcttc gggggtcgtg gcctcttcgg agccgacgat tcccaccgac
     6901 gcacgaaacg gtgcgacctc gcccaacgca aggtgaagga ccaacgattc ccgcacccgt
     6961 tccatcgccg cgaccgcgtg ttgcgcggag gttgacggaa agagacacac gaaacggtcg
     7021 tcgtcgtagc gagcgatgat gtcgctcggg cgaagggtgc gggtcagcac ctcggcgaac
     7081 gtccgaagcg cccggtcgcc gaggtccgcg ccgtggcaat cgttgtagtg acggaattcg
     7141 ttgaggtcgc acagcgcgag ggcaaacccg gtgtcggtgg accgcaggcg gtgaaggtcg
     7201 cc
//
LOCUS       DAOOMA010000050         7201 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_12316_length_7201_cov_4.022250, whole genome shotgun sequence.
ACCESSION   DAOOMA010000050 DAOOMA010000000
VERSION     DAOOMA010000050.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 7201)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7201)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7201
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12316_length_7201_cov_4.022250"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(90..926)
                     /locus_tag="PLP95_02450"
     CDS             complement(90..926)
                     /locus_tag="PLP95_02450"
                     /inference="COORDINATES: protein motif:HMM:NF022740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3097 family protein"
                     /protein_id="HPB44690.1"
                     /translation="MSSPHQRGILSSLDDIDNGPSKRKQYPKVPANLGTDVSHQATGI
                     VGAIVEYSDNVITIRDDYGKDHRYRNIPGSFLVDGRPIQLSRPTPSATAERRITASGS
                     IADPRNAPAKVAKASRILVEGTHDAELVEKVWGDDLRSEGIIVQPIDGVDDLGEIVAA
                     FGPRPGRRLGVLVDHLVDNSKESRIAASVDHPDVLICGHVFIDIWAAVNPRRIGLEAW
                     PDIERGTPWKEGICAAVGVEEPWQFWKLLLSKVDSYRDLDPSLVGAVEQLIDFVAEPA
                     EH"
     gene            complement(978..1286)
                     /gene="yaaA"
                     /locus_tag="PLP95_02455"
     CDS             complement(978..1286)
                     /gene="yaaA"
                     /locus_tag="PLP95_02455"
                     /inference="COORDINATES: protein motif:HMM:NF015820.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HPB44691.1"
                     /translation="MWDLLPNEHAAAWAPDPASMRSRISVKFIDETTDPATGETKRSV
                     VNHWNKLLKGALVRHVLDTQLCDHRGLADFDHPLGYRYDDSLTVERKGVTQVSLIAPR
                     "
     gene            complement(1801..1874)
                     /locus_tag="PLP95_02460"
     tRNA            complement(1801..1874)
                     /locus_tag="PLP95_02460"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1838..1840),aa:Lys,seq:ttt)
     gene            complement(1941..2414)
                     /locus_tag="PLP95_02465"
     CDS             complement(1941..2414)
                     /locus_tag="PLP95_02465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB44692.1"
                     /translation="MFAMSAIAVSAAQSATDVTTSDRFSELVTFFLIALAIVAPSTLI
                     VFRRMNRKGAGLRGEETTADPADTATGTTADTATGPEPDPAEVGSVIALIDLAAERLG
                     PGESSNIEVPEPLLLDGKPAPQAIADALLADAVARHRLSATWAHRDGVRTVTLTR"
     gene            complement(2658..3284)
                     /locus_tag="PLP95_02470"
     CDS             complement(2658..3284)
                     /locus_tag="PLP95_02470"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HPB44693.1"
                     /translation="MTRLLLIRHGQSEWNADGRWQGQANPPLTTLGRRQAAEASASLG
                     AVDGIFSSTLNRAHSTAEIIAAEIGIGPVIALPGLIERHAGEWQGLTRSQIEAQFPGY
                     LDENRRPPSWESDDSLAERAFDSLDRIVRALAELGARGEERSGVDVEAIVVTHGGLVY
                     EVERRLGATFERMGNLDGRYVNHVSGSWVLGERVDLLHGVDVTIPDQI"
     gene            3327..4127
                     /locus_tag="PLP95_02475"
     CDS             3327..4127
                     /locus_tag="PLP95_02475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB44694.1"
                     /translation="MPTLVDRLARTYLVRQRPKGPTMLFGVVWFGLLVGASLAGSWGL
                     ALLFGLLGALGALQAGVAWRSARARMNQPLAALIALVLPCAATFNNRVLALSIVVAVI
                     AAVVLGESMGKPTVASGGAVANNLVVASATLRVGLPLGFGGAAVVQLERIDLMAMVLL
                     VMVVSVYDCGHFLLGTTLRGRFAGVWAGLAGQAVVLFSTLTMNPDPFTSDGSVALVVV
                     LAALSCPLGQWLGSWMLPSAAAKAPALRRLDSWLIAAPVVWACAALAS"
     gene            4201..4770
                     /locus_tag="PLP95_02480"
     CDS             4201..4770
                     /locus_tag="PLP95_02480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB44695.1"
                     /translation="MSDTDGPQEQYIVITELDEIVHSTDVGSFASLPIEELRSLRDRL
                     TEIEGGLSFGRRLAQGRLDIVLAEFHSRVQGRADAAAELVERLPDVLAPQTRGAGSPR
                     PVREGDLAPFADEIIAALDEMVNAIELAKLHEFEIDALDDIAQRIGEFERNISVRRSE
                     VHRLIDDVQEEIIGRYRSGVVSVDDLLSD"
     gene            4836..5201
                     /locus_tag="PLP95_02485"
     CDS             4836..5201
                     /locus_tag="PLP95_02485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HPB44696.1"
                     /translation="MKDEIDKRWHDLGEFIREQRNVGQMSLRKLSELAGVSNPYLSQI
                     ERGLRRPSAEILQQIARALEISSETLYVRAGILDEPDTTPDLAAEIRRDPQLSEEQKK
                     TLVRIYESFRRENEAAGSA"
     gene            5263..6480
                     /locus_tag="PLP95_02490"
     CDS             5263..6480
                     /locus_tag="PLP95_02490"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HPB44697.1"
                     /translation="MTSVAMLSMHTSPLAQPGVGDSGGMNVYVRELAASLAQGGVPTT
                     VYVRAWDRSLPRRVDVEPGFEVVHVPAGRFDLAKEDLVGVVDSFADWVGNDIERVGAS
                     VLHANYWLSGVAGHRLKHELELPLVTTFHTLAAVKSSTGDLESSEREEAERAIIGCSD
                     AVCASNPVEVDQLLSLYDAEPSRIEIVPPGVDRAFFAPGDRGGARYALGLGEYPVLLF
                     VGRIQPLKGLTVAVEALAKMANRSARLVVVGGPSGVDGAAELDRVHAMIRRYDLGGRV
                     IFAPPQPHHMLSTYYRAADLTLVPSRSESFGLVAAESLACGTPIVATDVGGLSALVED
                     REIGRLLADRNPAAWACVLDELLDDPAGLRRMSIVAGERGRRFTWSTTAARLRRVYAD
                     LSSSKVPATCGVA"
     gene            6480..6947
                     /locus_tag="PLP95_02495"
     CDS             6480..6947
                     /locus_tag="PLP95_02495"
                     /inference="COORDINATES: protein motif:HMM:NF022182.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjN domain-containing protein"
                     /protein_id="HPB44698.1"
                     /translation="MHDDRPASSPEELERIAASIDGWAQRQIDEDWVAAVERPEPDKP
                     LWFIRALGEQKGVFTVWFMLRQRSLFIECYVMPSPEERRDEVFEYLLRRNLRMFGMKF
                     SIGNEDAVYLTAEIHNDDVCDAELDRLLGTAYMETEQSFRPAMRLGFGSKFKG"
BASE COUNT         1081 a         2166 c         2622 g         1332 t
ORIGIN      
        1 ccccggtggg aggagcgctt cgagcgcgac gaggaggccc gcgagcagcg attgcgcgag
       61 ctgatcggcg ttggcgagct gatcggggat cagtgctcgg cgggctctgc gacgaaatcg
      121 atgagctgtt cgaccgcccc caccaagctg gggtcgaggt ctcggtagct gtccaccttt
      181 gacaacagca gcttccagaa ctgccacggc tcttcgaccc cgacggcagc gcagataccc
      241 tctttccacg gggtaccgcg ctcgatgtcg ggccaggcct cgaggccgat ccggcgcggg
      301 ttgaccgcgg cccagatgtc gatgaacacg tgaccgcaga tcaaaacgtc gggatgatcg
      361 acgctggcgg cgattcggga ctccttggag ttgtcgacga ggtgatcgac gagcacgccg
      421 aggcgccggc cggggcgcgg cccgaacgcg gccacgatct cgccgaggtc gtcgacgccg
      481 tcgatgggtt gcacgatgat gccctccgac cgcagatcgt cgccccacac cttttcgacg
      541 agttcggcgt cgtgggttcc ctcgacgagg atgcggctgg ctttcgcgac cttggccggg
      601 gcgttgcgcg gatcggcgat cgatcccgat gcggtgatgc gccgctcggc ggttgccgag
      661 ggggtgggtc ggctcagttg gatcggccga ccgtcgacga ggaagctgcc ggggatgttc
      721 cggtagcggt ggtccttgcc gtagtcgtcg cggatcgtga tgacgttgtc gctgtattcg
      781 acgatcgcgc cgacgatgcc ggtggcctga tggcttacgt cggtgccgag attcgccgga
      841 accttggggt actgcttgcg cttggacggc ccgttgtcga tgtcgtcgag gctggagaga
      901 atgcctcgct ggtgggggga actcatcggg gtcgatcgta accgtacggt gccgccagcc
      961 cggtcagcgc ggcgcggtca gcgcggcgcg atgaggctca cctgcgtgac gcccttgcgc
     1021 tcgaccgtca gcgagtcgtc gtagcggtaa ccgagcgggt ggtcgaagtc ggcgagcccg
     1081 cggtgatcgc acagctgggt gtcgaggacg tggcgaacga gggcgccttt gaggagcttg
     1141 ttccagtgat tgacgaccga acgtttggtc tcgccggtgg cggggtcggt cgtctcgtcg
     1201 atgaacttca ccgagattct cgagcgcatc gacgccgggt ccggcgccca ggccgccgcg
     1261 tgttcgttgg ggagcaggtc ccacacggtg cgcctcgacc accggggcga gggcgtcggt
     1321 gagcggctga cgccaggtgg ttgccaactt tccgaccccg gggagcgagg cgcccatctt
     1381 gagtttgtag tcggggatcg gatcggctgc ggcgaccgcg ccccagagac ccgaaaagat
     1441 gatgatctgc ttggcagccc ggcgtttcgc cgtcgccgac agcgtcgcga agtcgagggc
     1501 gtcgtagagc acgccggtgt accgttcgac ggcccgtagc gtcggcgaga ccatcacgtt
     1561 gcggttggcg gcgcgtgccg cttcgagcgc agcgcccttg accccgagca gtttggcggc
     1621 cttcgcgtcg tcggccatgg cgtcgctcag cgcagctgca actcgaaccc ggaggggatc
     1681 gagttcgtcg aatgacaacg atgcctcgga aaaagcgggg cccgttccgc cggccgactt
     1741 gccttccgag gggggcaaca gaatgagggg agccttggac gtcatggtcg ggtgtgtcgg
     1801 tgcccccact cggattcgaa ccgaggacca atggattaaa agtccactgc gctaaccagc
     1861 tgcgccatag gggcgcgacg acagtaccag gctggacccg cagcggtgaa ggcgtgacgc
     1921 ggtgggtcgg cggctgaagg tcaacgggtg agggtgacgg tgcggacgcc gtcgcggtga
     1981 gcccaggtgg cgctcaggcg atgcctggcg acggcgtcgg cgaggagcgc gtcggcgatg
     2041 gcctgcggtg cgggcttgcc gtcgagcagg aggggctcgg ggacctcgat gttcgatgac
     2101 tcgccgggac ccaaccgttc ggccgccagg tcgatgaggg cgataaccga cccgacctcg
     2161 gccgggtcgg gctcggggcc tgtcgcggtg tcggccgtcg tgccggtcgc ggtgtcggcg
     2221 gggtcggccg tggtctcctc cccccgcaga ccggctccct tgcggttcat gcgccgaaac
     2281 acgatcaggg tcgacggcgc aacgatcgcg agcgcgatga ggaagaacgt caccaactcc
     2341 gagaatcggt cgctcgtcgt gacgtcggtt gcggactgcg ctgccgacac ggcgatggcg
     2401 gacatggcga acatctcgcg ggatcctacc cagaggtgtg ggcggttcgg gggtgtccta
     2461 cgctgggcga cgtgcaggat cagcccatca tcgacgaagc actcctcgag cgcctcgagc
     2521 gtgaacttgc cgacgccgcc gacacgctcg aacaggtcga gcggatcgtt gccagcgagc
     2581 tcaccggtgc cgagtcggcc gcagcgattc gcgggctcct cgacgacgga cgtttcgccg
     2641 tgcaggagcc cactgactca gatctggtcg gggatggtga cgtcgacgcc gtgcaacagg
     2701 tcgacgcgct cgccgaggac ccacgagccg ctgacatggt tgacgtaacg gccgtcgagg
     2761 ttgcccatcc gctcgaaggt ggccccgaga cgccgctcga cctcgtagac caacccgccg
     2821 tgggtcacga cgatggcttc gacatcgacc ccggagcgtt cttcgccgcg ggcacccaac
     2881 tcggcgaggg cacgcacgat tcgatcgagt gagtcaaagg ctcgctcggc gagagaatcg
     2941 tcgctctccc aactcggcgg acgccgattt tcgtcgaggt acccgggaaa ctgcgcctcg
     3001 atctgagagc gggtgagccc ctgccattcg ccggcatggc gttcgatgag cccgggcaag
     3061 gcgatgaccg gaccgatccc gatctccgcg gcgatgatct ccgcggtcga atgggcccgg
     3121 ttcagggtgg aggagaagat cccgtcgacc gcaccgagcg acgcgctggc ctccgcggcc
     3181 tgtcgacgcc cgagggtggt cagcggcggg ttggcctggc cctgccagcg gccatcggcg
     3241 ttccattcgg attgtccgtg tcggatcaac agaagtcgcg tcatcgaagc aacggtaggg
     3301 ccagggccgc ggactacgct cgtttcgtgc ccacactggt cgaccggttg gcccggacct
     3361 acctggtgcg tcaacggccg aagggcccga cgatgctgtt cggggttgtc tggttcggcc
     3421 tgttggtcgg ggcgtcgctg gcgggatcgt gggggttggc cctgctgttt ggtctgttgg
     3481 gggcgctcgg ggcgttgcag gcgggggtgg cgtggcgttc ggcgcgggcg cggatgaacc
     3541 agccgcttgc ggcgttgatc gcgctcgtgc tgccgtgtgc cgcgacgttc aacaatcggg
     3601 tgttggcgct gtcgatcgtc gtcgccgtga tcgccgccgt cgtcttgggt gagtcgatgg
     3661 gcaagccgac ggtggcaagt ggtggagcgg tcgctaacaa cctggtcgtg gcatcggcga
     3721 cgttgcgggt ggggttgccg ctcgggttcg gcggtgccgc ggtggttcag ctcgagcgga
     3781 tcgatctgat ggccatggtg ctgttggtca tggtggtgag tgtctacgac tgcgggcact
     3841 tcctcctggg caccacgctg cggggacgct ttgccggagt gtgggccggg ttggccggtc
     3901 aggccgtcgt gttgttttcc acgctgacga tgaaccccga tccgttcacc tccgacggtt
     3961 cggtggccct cgtggtcgtg ctcgcggcgt tgtcatgtcc actcgggcaa tggttgggaa
     4021 gctggatgtt gccgagtgcg gcggcgaagg ccccggcgtt acgccgcctc gactcgtggt
     4081 tgatcgcggc tcccgtggtg tgggcgtgcg cggcactagc gtcgtagcga tcatgagacc
     4141 cgcaacctct taccggcgcg ccacctcagg ttcgcgtccg gcctgccgat ggagaatctg
     4201 ttgagcgaca ccgacggtcc ccaggagcag tacatcgtga tcaccgaact cgacgagatc
     4261 gttcactcca ccgacgtggg gtcgttcgcc tcgcttccga tcgaggaact gcgcagtttg
     4321 cgcgaccgtt tgaccgaaat tgagggtggg ttgtccttcg gccggcgttt ggcccagggc
     4381 cgcctcgaca tcgtcttggc cgagtttcat tcgcgggtgc agggccgcgc cgatgctgcg
     4441 gcggaattgg tcgaacgtct gccggacgtg ttggcgccac agacccgtgg cgccggatcg
     4501 cctcggccgg ttcgtgaagg cgacctcgcc ccgttcgccg atgagatcat cgcagcgctc
     4561 gacgagatgg tgaacgcgat cgaactcgcg aagttgcacg agttcgaaat cgacgccctc
     4621 gacgacatcg cccagcggat cggcgagttc gagcgcaaca tttcggtgcg tcgcagcgag
     4681 gtgcaccggc tcatcgacga cgtccaagag gagatcatcg gtcgataccg ttcgggcgtt
     4741 gtctcggttg acgatctgct ttccgactga gcatcgccgt gactccgcct cggctcggtt
     4801 cggccgcgac gcccccatct tctaacgttg acgccatgaa ggacgagatc gacaagcgtt
     4861 ggcatgacct cggtgagttc attcgcgagc agcgaaatgt cggtcagatg tcgcttcgca
     4921 agttgagcga actcgctggt gtgtcgaacc cgtacctcag ccagatcgaa cggggtctgc
     4981 ggcgtccgtc agcggagatc cttcagcaga tcgcccgcgc gctcgagatc agttccgaga
     5041 cgttgtacgt gcgcgccggg attcttgacg aacccgacac cacccccgat ctcgccgccg
     5101 agatccgtcg tgatccgcag ctcagcgagg aacagaagaa gaccctcgtg cggatctacg
     5161 agtcgttccg gcgtgaaaac gaggccgccg ggtcggcgta gggccgtcag cggtgatttt
     5221 gcgacgggaa tcaaccgggc gcgttgcgtg ttggaggctt gcgtgacctc cgtggcgatg
     5281 ctttcgatgc acacgtcgcc gctcgcgcaa ccaggcgtgg gcgacagcgg gggtatgaat
     5341 gtgtacgtgc gggagctggc ggcgtcgctg gcccagggtg gggttcccac cacggtgtac
     5401 gtgcgggcgt gggaccgctc gctgccccgt cgggtcgacg tcgagccggg ctttgaggtc
     5461 gtgcacgtcc cggcggggcg tttcgacctc gccaaggagg atctcgtcgg ggtcgtcgat
     5521 tcgttcgcgg actgggtcgg aaacgacatc gagcgggtcg gagcgtcggt gctgcacgcc
     5581 aactactggt tgagtggagt cgccggccac cgcctgaagc acgagttgga gctgccgctc
     5641 gtcacgacgt ttcacacgct ggcagcggtg aagagctcga cgggcgatct tgaatcgtcc
     5701 gagcgcgagg aggcggagcg ggccatcatc ggctgttccg atgcggtgtg cgcgtcgaat
     5761 ccggtggaag tcgatcagct gttgtcgctc tatgacgctg agccgagccg aatcgagatc
     5821 gtgcccccgg gggtcgaccg ggcgttcttt gctcccggtg atcgcggtgg cgccaggtac
     5881 gcgctcggtc tgggcgagta cccggtgttg ttgttcgtgg gtcgcattca gccgctcaag
     5941 ggactgacgg tggcggtgga ggccttggcg aagatggcga atcggtcggc ccgcctggtc
     6001 gtggtcggag gcccgagcgg ggtcgacgga gcggccgaac tcgaccgggt tcatgcgatg
     6061 atccgtcgct acgacctggg cgggcgggtg attttcgctc cgcctcagcc gcaccacatg
     6121 ttgtcgacct actaccgggc cgccgatctg acgttggtgc cgagccgatc ggagagcttc
     6181 ggtttggtcg cggccgagtc gctcgcgtgc ggaactccga tcgtcgcgac cgacgtcgga
     6241 gggttgtcgg cgctggtcga ggaccgcgag atcgggcgcc tcctggccga ccggaacccc
     6301 gccgcatggg cgtgtgtgct ggacgagctg ctggacgatc cggcggggct tcgccggatg
     6361 tctatcgtgg cgggtgaacg cggtcggcgc ttcacgtggt cgacgaccgc ggcccggctt
     6421 cgacgggtgt acgcagacct gtcgagttcg aaggtcccgg cgacgtgtgg agtggcctga
     6481 tgcatgatga tcgaccagcc agttcccccg aggagttgga acggatagcg gcctcgatcg
     6541 atggctgggc gcagagacag atcgacgagg actgggtcgc tgcggtcgag cgacctgaac
     6601 ccgataagcc gctgtggttc attcgggcgc tcggggagca aaagggcgtg ttcaccgtgt
     6661 ggttcatgct tcggcagcga tcgttgttca tcgagtgcta cgtcatgccg tcgcccgagg
     6721 agcgccgcga cgaggtgttt gagtatctcc tgaggcggaa tctgcggatg ttcggcatga
     6781 agttctcaat cggcaacgag gacgcggtgt atctcacggc cgagattcac aacgacgacg
     6841 tctgcgacgc cgaactcgac cgcctgttgg gcacggccta catggagacc gagcagagct
     6901 tccggccggc catgcgcctg ggcttcggct cgaagttcaa gggctgaccc gctcccgatc
     6961 cggccgacct cccggccgat cagcgaaggc gtggaagttt gtgcgacgaa gtggttgctt
     7021 ttccgcgggg agttcggcaa cgtaacgcca cggccggagg gtgcggcgcc ggacgtcggg
     7081 gaagcggacc gatgcatgca ccctccggcc tcttgcttcc cggcctcttg ttacccggcc
     7141 tcttgttctt tgtggcgggg ggtgttggtg atggtggtgt tgtcgggtcg ggtggcttgg
     7201 a
//
LOCUS       DAOOMA010000051         7159 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_12403_length_7159_cov_4.375563, whole genome shotgun sequence.
ACCESSION   DAOOMA010000051 DAOOMA010000000
VERSION     DAOOMA010000051.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 7159)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7159)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7159
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12403_length_7159_cov_4.375563"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1077)
                     /locus_tag="PLP95_02500"
     CDS             complement(<1..1077)
                     /locus_tag="PLP95_02500"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: protein motif:HMM:NF005568.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006780 - uroporphyrinogen III biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HPB44699.1"
                     /translation="MVDPLTGFVVAITGHRRWEEQHEMLARRGATVLHGPVMHTSLLD
                     DVDATVAATRAALDRRPDLLVLTTGLGTRSWFAACESARLDEALLAWCGDATVIARGP
                     KARSAALGCGIEVDWHAASETGAEVLEHLLGLGVADRNIVVQRDGGEPLLADELTKAG
                     ANVVDIPVYQWTPPHDPAPALRLIDAVLDDRVDAVTFTCSYAVQSTLRLATDPGEFVA
                     ALGRRARAVAVGPVTAQALRDAGVTRVVEPRRARLGAMVQALITEVSGTAVRLSVGGE
                     SAELRGNALVESSGAVRLLTPRERQLLMRLLSAAPGVLTKEELAGDGVDPHAVEAAIG
                     RLRTKLGVLRSGIVSVPRRGYRSTL"
     gene            complement(1142..2581)
                     /locus_tag="PLP95_02505"
     CDS             complement(1142..2581)
                     /locus_tag="PLP95_02505"
                     /inference="COORDINATES: protein motif:HMM:NF015692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HPB44700.1"
                     /translation="MMTSRERSTGALLGLALGDAVGTTLEFRSPGTFEPIDDLIGGGP
                     FGLKPGEWTDDTTMAMCLGESILDTGELDLADQLRRYLLWRDEGYWSVKDRCFDIGNA
                     TSAALARFARDGSVVDAVIDEEAAANGSLMRLAPVPIRWHQDVGLAAEKSAESSRTTH
                     PARRPVDACRVYGAMTAALIGGAAPDEVLSPEFWQWGELDPQIEAVVRGSWRTKSDSE
                     IRGTGFVVDALEAALWAVGGANSFADAVLRAANLGDDADTTAAIAGQLAGAIWGESEM
                     PTQWLAKLAQRDRIRAVAARLHDQAVGATAAQPWPFDQFVHGYWIEPGRVLAGEYPGD
                     PSAGKADAKVNLLVDNGVRTFVNLTTEHDPLAPYDHYVALAGSRRNLDLRVVSHGIPD
                     MGVRPAESYDEIIETIRAESERGVVYVHCWGGIGRTATVAGCLLVDGGMSSMEALDFI
                     RAQRSVTRKAHREAPQTPAQRRVIERRAR"
     gene            2803..4026
                     /locus_tag="PLP95_02510"
     CDS             2803..4026
                     /locus_tag="PLP95_02510"
                     /inference="COORDINATES: protein motif:HMM:NF017538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqiA/YcfP family alpha/beta fold hydrolase"
                     /protein_id="HPB44701.1"
                     /translation="MTNRRVEQTHPQSGLKLLALPAALWRTGAYFAPSHYLRRQSWHS
                     RASLPAPSPSLGGVAAVLADELVLAGFKLTRNPPTVDAWERISAEVADALISFEHEGW
                     LENPASYHRAPVVPNDATVTPVQRWESLGMRWQQLRWTSGWEPVVGQPGAQRWSSYER
                     NHRASAWMLRYPDDKPRHWAVLVHGTEQGRLLVDQIVFRARRLHYELGCNVLMPLLPL
                     HASRRAPDALGTGFPTLDVMDNIHGLAQSAYDVRCALAWVAEQEPSSVSLTGLSLGGY
                     TAALVAGLEEPLGAVVGLVPAVDFPEVFRRQTPHLMRYSEAFAALHEASRRLHSVVSP
                     LSFTPQTPSERLYVLAGIHDRLLDPLNQAASLAEHWGTDNVVWLDRGHVSHMTSPELG
                     SIIESAVTSVPVPAT"
     gene            complement(4057..5649)
                     /locus_tag="PLP95_02515"
     CDS             complement(4057..5649)
                     /locus_tag="PLP95_02515"
                     /inference="COORDINATES: protein motif:HMM:TIGR02946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="wax ester/triacylglycerol synthase family
                     O-acyltransferase"
                     /protein_id="HPB44702.1"
                     /translation="MRKMSGQDAAFLYGETPNWHFHVNGLMIVDAASAPDGWTFERFR
                     ELLISRLPEVPQLRWRYVNVPFGLDRPSWVEAHDIDPDYHIRRVALPSPGGERELNAT
                     VGRIISYKLDRTRPLWEAWCIEGLEGGRVAILHKMHHAIIDGVSGAGLADVLLDLQPT
                     PRVSPVEVHDEIEAERVPGPMEMLVRGVANSVVRTPFRTLRFAQQTALQFASAVPVLR
                     GENPVSLPLTAPRSVFNSDPTPRREFAATRVEFERVRAVKDAYGVKVNDVVLALVSGA
                     MRSYLIERGELPGSSLIASCPVSLRADSKDGPVGNHVGNMFASLATDIEDPAERLGRI
                     HQSTMSAKEMQKALAAHQIMGLTETTPPGLIALAARMYTSVGLASHTPPAASVVVSNV
                     PGPPIPLYLAGGRLEALYPMGPLLMGMSLNVTALSIMGSIDFGFMSCPDVVPHLRTMA
                     EAVPAALDELEAAAPRKRSTSTTKQAPAPKAAAKKAPAKKAPAKKTPAPKVAAKVPAK
                     KVPAKKSAAPKAPAKKTASTPG"
     gene            complement(5737..6717)
                     /locus_tag="PLP95_02520"
     CDS             complement(5737..6717)
                     /locus_tag="PLP95_02520"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HPB44703.1"
                     /translation="MKTLVIGATGFIGSAITRHLVDEGGEVRIAVRSGSDTRGLDDLD
                     VERVGADLRDIDSLRTALRGCDRLFLTAALFSHWARDVDAFYEVNVTGTRHALQAALE
                     ADVSKIVYTSTNNAIGAYGPTAVDEEVVFNYWNTGDHYSISKYLGEVEATRFAARGLA
                     VVIVNPTLVVGPGDRRPTSSGQLVLEVAGGKLPVYTQGWVNVVDVDDVAAGHLLAAER
                     GRVGERYLLGGTNLTVKEYFGLIAEAAGRRPPRVRLPYRTAVALAHGFEAVSRFTKRH
                     PTATVSEVKIGRLGEWYNSDKAQRELDFTARPAAEAIQRSVTWFKDEGYL"
     gene            complement(6821..>7159)
                     /locus_tag="PLP95_02525"
     CDS             complement(6821..>7159)
                     /locus_tag="PLP95_02525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020378469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HPB44704.1"
                     /translation="ADADGAPTPHLIAEEALDMAACIGCGACVAACPNGSAHLFAGAK
                     LAHLALVSRGRAERGRRARRIVDRLDSEFGPCSTHGECAEVCPASIPMTAVATVNRER
                     LRAALRRGDD"
BASE COUNT         1164 a         2514 c         2294 g         1187 t
ORIGIN      
        1 cagggtcgag cgataccccc gacgcggcac cgacacgatg cccgatcgca acacacccag
       61 cttggtgcga agccgcccga tggcggcttc caccgcgtgc gggtcgaccc cgtcgccggc
      121 gagttcctcc ttcgtcaaca cgccgggtgc ggcgctcaac agtcgcatga ggagttgacg
      181 ctcgcgtggg gtgagaaggc ggaccgcacc ggaactctcg acgagtgcgt tgccccgcaa
      241 ctcggcgctt tcgccgccga ccgacaaccg cacggcggtg cccgacacct cggtgatcag
      301 cgcctgcacc atcgccccca gccgggcgcg acgaggttcg accacgcggg tcaccccagc
      361 gtcgcgcagc gcctgcgcag tgaccgggcc gacggcgacg gcgcgggctc gacggccgag
      421 tgccgccacg aactcacccg gatcggttgc gagccggagg gtggattgaa ccgcatagct
      481 gcaggtgaac gtgacggcgt cgacgcggtc gtcgagcacc gcgtcgatca ggcgcaatgc
      541 cggcgcgggg tcgtggggcg gcgtccattg atagacggga atgtcgacga cgttcgcgcc
      601 cgccttcgtc agttcgtcgg ccaacagcgg ctcgccaccg tcgcgttgca ccacgatgtt
      661 gcggtctgcc acaccgaggc cgagcaagtg ttcgagtacc tcggcgccgg tctccgaagc
      721 agcgtgccag tcgacttcga tgccgcatcc gagcgcggcc gagcgcgcct tgggcccgcg
      781 tgcgatcacg gttgcgtcgc cgcaccacgc gagcaacgcc tcgtcgagtc gggcactttc
      841 gcacgcagcg aaccacgatc gcgtccccaa accggtcgtg agcaccaaca ggtcgggacg
      901 tcgatcgagc gcagctcgtg tcgcagcgac cgtggcgtcg acgtcatcga gcaggcttgt
      961 gtgcatcacc gggccgtgca gcaccgtggc accgcgccga gccagcatct cgtgttgttc
     1021 ctcccatcgc cggtgcccgg tgatggcgac cacgaaaccg gtgagcggat cgaccatgcc
     1081 gggacgctaa ccgggcaatg tttcggcacg gtttcctggc cgactccagc gacaggtact
     1141 gctaccgggc ccgtcgctcg atcacccgcc gctgagccgg ggtctgaggc gcctcacggt
     1201 gagcctttcg tgtcaccgac cgttgagccc tgatgaagtc gagcgcttcc atcgacgaca
     1261 tcccaccgtc gacgagcagg caaccggcga cggtcgccgt tcgtccgatc ccgccccagc
     1321 agtggacata caccacaccc cgctcggact ccgcgcgaat ggtctcgatg atctcgtcgt
     1381 agctctcggc cgggcgaacc cccatgtcgg ggatcccgtg cgacacgacc cggaggtcga
     1441 ggtttcggcg ggatccggct agcgcgacgt agtggtcgta gggcgcaagg gggtcgtgtt
     1501 cggtcgtcag gttcacgaac gttcgaacac cgttgtcgac caacaggttg acctttgcgt
     1561 cggcctttcc ggcagagggg tcgcccgggt attcgcccgc cagaacccgt ccgggctcga
     1621 tccagtagcc gtggacgaac tgatcaaacg gccatggttg tgcggcggtg gcgccgacgg
     1681 cctgatcgtg aagtcgagcg gccacggcac gaattcggtc gcgttgggcc agcttcgcca
     1741 accactgcgt cggcatctcc gactcgcccc agatggcgcc ggcaagctgt ccggcgattg
     1801 ccgcggtcgt atcggcgtcg tcacccaggt tggccgctcg cagcaccgcg tcggcgaagc
     1861 tgttcgcccc accaaccgcc cataacgccg cttcgagtgc gtcgaccacg aacccggtgc
     1921 cgcgaatctc cgaatcggat ttcgtcctcc acgacccgcg aacgaccgcc tcgatctgtg
     1981 ggtcgagttc accccattgc cagaactcag gcgacagcac ctcgtctggg gcggcgccac
     2041 cgatgagcgc ggcggtcatc gctccgtaga cccgacacgc atccaccggt cggcgcgcag
     2101 ggtgggtcgt ccggctggac tcggccgact tctccgccgc gagtccgacg tcttggtgcc
     2161 aacggatcgg caccggggcc agccgcatga gcgacccgtt cgccgccgcc tcctcatcga
     2221 tgacggcgtc gacgaccgac ccgtcgcgag cgaagcgggc gagcgctgcg gaggtggcat
     2281 tgccgatatc gaaacagcgg tctttgaccg accagtaacc ctcatcgcgc cacaacaggt
     2341 agcggcgcag ttgatcggcg aggtcgagct cgccggtatc gaggatcgac tcgccgagac
     2401 acatcgccat cgtcgtgtcg tcggtccatt cccctggctt cagtccgaac ggtccacctc
     2461 cgatgaggtc gtcgatcggt tcgaacgtcc cgggagaacg aaactccaac gtcgttccca
     2521 cggcgtcgcc gagcgccaat cccagcagcg cacctgttga tcgttcccgc gaagtcatca
     2581 tcgcccgatc gtgccagagc ccctgccgcc tgtgccgagc aatttccgcc agggctccgg
     2641 acacaccgca acggtcgggc ccgaactttg tgcagaaagc tcgcgacggc gggacgggag
     2701 gtttgagcca gccagcagct cggaggttga accgccgact gcgactcagt taaaccgatg
     2761 gggtcgcgtc gcttgccggc gggcccgacc aaggagctgc acatgacgaa ccgtcgagtc
     2821 gagcagaccc atccgcagag cggcttgaag cttctcgccc tgcctgctgc actgtggcgc
     2881 accggcgcct acttcgcccc ctcccactat ctgcgccgcc aatcctggca cagccgcgcg
     2941 agcctgccgg caccgtcgcc gtcgctcggt ggagtggcag cggtgctcgc ggatgagctg
     3001 gtcctcgccg gtttcaaact cactcgcaac cccccgacgg tcgacgcgtg ggagcggatt
     3061 tccgcagagg ttgccgacgc gctgatctcg ttcgaacacg agggatggct agagaaccct
     3121 gcgtcctacc accgtgcgcc ggtggtcccg aacgacgcca ccgtgacgcc ggtgcaacgc
     3181 tgggaatcgc tgggaatgcg ttggcagcag cttcgctgga ccagtggctg ggaacccgtg
     3241 gtcggccagc cgggtgcgca acgttggagt tcgtacgagc gaaatcaccg ggcgtcggcg
     3301 tggatgctcc gctaccccga cgacaagcca cgccattggg cggtgctcgt ccacggcacc
     3361 gaacagggac gtctgctcgt cgaccagatc gtgtttcgag cccgccgcct gcactacgaa
     3421 ctcggctgca acgtcctcat gccgctgttg ccgctccatg cctcgcgtcg cgcgcccgac
     3481 gcgctcggaa cgggatttcc caccctcgac gtcatggaca acatccatgg cctcgcccag
     3541 tcggcctacg acgttcgctg tgccttggcg tgggtcgccg aacaggaacc cagcagcgtc
     3601 tcactcacgg gcctgtcgct gggcggctac accgccgctc ttgtcgccgg cctggaagaa
     3661 cccctcggcg cagtcgtcgg cctcgttccg gcggtcgatt ttcccgaggt gttccgccgc
     3721 cagaccccgc atctgatgcg ctacagcgag gcgttcgcag cgttgcacga ggcctcgcgg
     3781 cggctccact cggtcgtgtc gccgttgtcg ttcaccccgc agactccaag cgagcggctc
     3841 tacgtgctcg ccggcatcca cgatcgcctc ctcgacccgc tcaaccaggc tgcgtcgctt
     3901 gccgagcact ggggaaccga caacgtcgtg tggcttgacc gcggccacgt ctcacacatg
     3961 acctcaccgg aactcggttc catcatcgag tccgcggtca cctccgtgcc cgtgccggcc
     4021 acctgaacga ccactgccgg tcggggtcgc ctcgcttcag ccaggagtcg acgcggtctt
     4081 cttcgctggg gcctttgggg cagcagactt cttggctggc accttcttgg ccggcacctt
     4141 ggccgcgacc ttcggtgccg gcgtcttttt ggcgggcgcc ttcttggctg gcgccttttt
     4201 ggccgcggcc ttcggtgccg gcgcctgttt cgtcgtcgac gtgctgcgct tgcgcggtgc
     4261 cgccgcttcg agttcgtcca acgccgccgg cacggcctcg gccatcgtcc gaaggtgagg
     4321 tacgacatct ggacaactca tgaagccgaa atcgatgctg cccataatcg acagcgccgt
     4381 cacgttcagg ctcatcccca tcagcagcgg gcccatcgga tagagcgcct cgagtcggcc
     4441 acccgcgagg tacagcggaa tcggtggtcc cgggacgttc gagacgacga ccgacgcggc
     4501 gggaggtgta tgcgatgcga gccccacact ggtgtacatc cgggctgcca acgcgatcag
     4561 gcccggaggg gtggtttcgg tcaggcccat gatctgatgc gctgcgagcg ccttttgcat
     4621 ctccttggcc gacatcgtcg actggtggat ccgaccgagc cgctccgccg gatcctcgat
     4681 gtcggtcgcg aggctggcga acatgttgcc gacgtggttg ccgacagggc catccttgga
     4741 atcggccctc agcgaaaccg gacaggatgc gatcagtgag gaaccgggca gctctccccg
     4801 ctcgatgagg tagctgcgca tcgcccccga caccaacgcc agcaccacgt cgttcacctt
     4861 gaccccgtag gcgtccttca ccgcccgcac acgctcgaac tcaactcggg tcgccgcgaa
     4921 ctcacgacgt ggggtcgggt cgctgttgaa aaccgagcgc ggtgcggtca gcggcaggct
     4981 gaccgggttc tcaccgcgca gaaccggaac cgcggacgcg aactgcaaag cggtctgttg
     5041 ggcgaagcgc agtgtccgaa acggagtgcg gaccaccgag ttggcgactc cccgcacgag
     5101 catctccatc ggacccggca cccgctcggc ctcgatctcg tcgtgcacct cgaccgggga
     5161 aacacgcggc gtcggctgga ggtccaacaa cacgtcggcg agaccggccc cggacacccc
     5221 gtcgatgatc gcgtggtgca tcttgtgcag gatcgcgacg cgcccgcctt cgagcccctc
     5281 gatgcaccac gcctcccaca acggccgggt gcgatcgagc ttgtaggaga tgattcgccc
     5341 gaccgtcgcg ttgagttcgc gctctcctcc ggggctcggc aacgccacgc ggcggatgtg
     5401 atagtcgggg tcgatgtcgt gggcttccac ccagctcgga cggtcgagac cgaacggaac
     5461 attgacgtac cgccagcgca attggggcac ctcgggcagt cgtgagatca gcagctcccg
     5521 gaaccgttcg aaggtccagc cgtcaggagc gctcgccgcg tcgacgatca tcaggccgtt
     5581 cacgtggaaa tgccagttgg gggtttcgcc gtacaggaat gccgcgtcct ggccgctcat
     5641 cttgcgcatc cgccttctat aacgcgtctt ggcgctcggc gctactgact ctggtcacca
     5701 aagccgcggg ctcgtcgaac cggggtatcc ggatgctcag aggtagccct cgtctttgaa
     5761 ccaggtgacc gatcgttgaa tggcctcggc ggcgggacgg gcggtgaagt cgagctcccg
     5821 ttgagccttg tcactgttgt accactcgcc gagccggccg atcttcacct ccgacaccgt
     5881 ggccgtcggg tgccgcttcg tgaaccgcga caccgcttcg aacccgtggg ccaacgctac
     5941 ggcagtccgg tagggaagcc gcacccgagg gggtcggcgc ccggccgcct cggcgatcaa
     6001 cccgaagtac tccttgacgg tgaggttggt gcctccaagc aggtaccgct cgccgacccg
     6061 gccccgttcg gcggccagga gatgtcccgc cgccacgtcg tcgacgtcca ccacgttcac
     6121 ccagccctgc gtgtagacgg ggagctttcc gccggccacc tccaacacga gctgtcccga
     6181 cgaggtcggc cgccggtcgc cagggccgac gacgagggtc gggttgacga tcaccaccgc
     6241 aagccctcgc gctgcgaaac gggtcgcctc cacctcgccg aggtacttcg atatcgaata
     6301 gtggtcgccg gtgttccagt agttgaacac gacctcctca tcgaccgcgg tcggcccgta
     6361 ggccccgatg gcgttgttcg tgctcgtgta gacgatcttc gacacatcgg cttccagcgc
     6421 agcctgcagc gcgtgacggg tgccggtgac gttcacctcg tagaaggcgt cgacgtcgcg
     6481 cgcccaatgc gagaacaatg cggcggtcag gaagagccga tcgcatccgc gaagcgccgt
     6541 ccggagtgag tcgatgtcgc gaagatcggc accgacgcgc tcgacgtcga ggtcgtccaa
     6601 cccccgggtg tcgctgccgg aacgcaccgc gatgcgaacc tcgccgcctt cgtcgacgag
     6661 atgtcgggtg atcgccgagc cgatgaatcc ggtcgccccg atcacgagcg tcttcatctg
     6721 cctgcacctc cgtcacgatc cgacctggtt cgaacactcg accgtatccg gcgaagacgc
     6781 catcctcgcc accgtcgaac tcgacggccg acgacaccgg tcagtcgtcg cctcgccgca
     6841 atgccgcccg aaggcgctcg cggttgacgg tcgcaaccgc ggtcatcggg atcgacgccg
     6901 ggcacacctc ggcacattcg ccgtgggtcg agcacggacc gaactcggaa tccagtcggt
     6961 cgacgattcg gcgggcccgt cggccacgtt cggcccgtcc ccgagacacg agggcaaggt
     7021 gggcgagctt ggcgcccgcg aacaaatggg cggaaccgtt cggacatgcg gcaacgcacg
     7081 ccccgcatcc gatacacgcc gccatgtcga gcgcctcctc ggcgatgagg tgaggcgtgg
     7141 gggcaccgtc cgcgtcggc
//
LOCUS       DAOOMA010000052         7148 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_12424_length_7148_cov_4.182433, whole genome shotgun sequence.
ACCESSION   DAOOMA010000052 DAOOMA010000000
VERSION     DAOOMA010000052.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 7148)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7148)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7148
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12424_length_7148_cov_4.182433"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..312)
                     /locus_tag="PLP95_02530"
     CDS             complement(<1..312)
                     /locus_tag="PLP95_02530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB44705.1"
                     /translation="MNSMHPTQTSEQLGVSNLKMVMRGLLIRCPACGHGNTHHRYFKM
                     RPECPTCTLKFERIFGHSLGYIGLNTIVTFTATFLVILIGSLLTYQNEGGIPGLPLLL
                     AA"
     gene            complement(347..829)
                     /locus_tag="PLP95_02535"
     CDS             complement(347..829)
                     /locus_tag="PLP95_02535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase"
                     /protein_id="HPB44706.1"
                     /translation="MEAVTTGPREDLDYATFGVAVREVSQQVVDSGYQPDWIVAIARG
                     GLLIGGAVSYALSCKNIGVVNVEFYSDVDERLDVPVMLPPALNLADLADTKLLIVDDV
                     ADTGETLKLVVDTCAPVVAEIRSLVIYEKPRSLLSPDYVWKRTDDWINFPWSSEPPLR
                     "
     gene            complement(864..1325)
                     /locus_tag="PLP95_02540"
     CDS             complement(864..1325)
                     /locus_tag="PLP95_02540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB44707.1"
                     /translation="MTRSRDTGRPDADDPQQFLVYHAEDSVIDQIGPRLRRWREVESF
                     VESVLISPGYLDAFPDAPLDVALERRSRSARSSLAVAGGDIILIRDGSWNALTVLHEL
                     AHLVVATTVPQSEPHGPAFVSTELELVRLRCGFDQYGSLRAAFQSAGVRIG"
     gene            complement(1322..2128)
                     /locus_tag="PLP95_02545"
     CDS             complement(1322..2128)
                     /locus_tag="PLP95_02545"
                     /inference="COORDINATES: protein motif:HMM:NF022432.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2786 domain-containing protein"
                     /protein_id="HPB44708.1"
                     /translation="MAQLRDSDARLDTIRALLAKADATTFEAEAEAFTAKATELMARY
                     AIDAAMLWSGESEADREVPDEIHLTLLRPFTAQKSILVAGVASAFGCQALRLRRGGQG
                     GEVISVVGFPSDLTLVETLITSLLVQLSTAMVHSPDQPRSATSSQVSSWRRSFISAFA
                     DTVIRRLIQDRRGAMDAVANQADGPSTNQGPSERGAAGSRSTDLVLADRADAVHTDFR
                     RRYPNIRTSRVSAGTSRSGHRAGTAAGNRADIGHTRLGARPALPGRRASP"
     gene            2289..2951
                     /locus_tag="PLP95_02550"
     CDS             2289..2951
                     /locus_tag="PLP95_02550"
                     /inference="COORDINATES: protein motif:HMM:NF025380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4230 domain-containing protein"
                     /protein_id="HPB44709.1"
                     /translation="MARHIWKLLGLAGAAVLVVNLLGVNPLGWVADGLWASDDPPAAA
                     DTTLLSITKTQDLRAATGEFSVPVYFGTEQNGFLKKIIPDAFDADSGIALYQGSVDAF
                     IDLSGLTEDDLEIDEAANTLTITVPDPTLSTPRLDHEKSSVVSQDRGVLTRLGEAFSE
                     SPMEGRDKLDETAVNELAKAAAQSNLLDTARTNGEDFLVALGEQLGFDRVIIVFEKPS
                     NP"
     gene            complement(2991..3899)
                     /locus_tag="PLP95_02555"
     CDS             complement(2991..3899)
                     /locus_tag="PLP95_02555"
                     /inference="COORDINATES: protein motif:HMM:NF019474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HPB44710.1"
                     /translation="MSADRFHPELRLARFVPRSPITQRTMPTIQRLTCLAGRRPPIGG
                     KIERCSATSSVRVFRPTDPTLPSTGRPALLWMHGGGLIIGSASMADGVLRHIARRHGA
                     VCASVEYRLAPQHPFPAPLEDCWDALVWLSRQPDVDPNRIAIGGESAGGGLAAALAIL
                     ARERGEVQPCFQVLSYPMLDDRSSDRDDIAPSTLRLWGTTSNRLGWDSYLGGRRPEEL
                     SAWAVPGRCEDLTGLPSAWVGVGTNDLFHDEDLDYACRLREAGSDVELMVVEGAYHGF
                     DLERWAPVAKAYRRAQIDALVTAWNR"
     gene            complement(3916..4686)
                     /locus_tag="PLP95_02560"
     CDS             complement(3916..4686)
                     /locus_tag="PLP95_02560"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HPB44711.1"
                     /translation="MNISGSTVLLTGASGGLGHAIARRLVREGATLILTGRREAELNQ
                     LADELDASVVVADLTDPTSIATLIEAASAADILIANAGTGNDVGIRSETAENIDRVID
                     TNLRSPIHLSVEFAQRLIDEGRTGHIVFVGSLSGLAPTPNTRLYNSTKFGLRGFALAM
                     RHDVAEHGIGVSIVEPSFIATAGMFADNGVQLPRVVRMKSPDDVAAGVVRAIQRNTAE
                     SFVSPIELRAVATLAVAAPNLSDRIQRLVDTKSFKPAD"
     gene            4765..6849
                     /locus_tag="PLP95_02565"
     CDS             4765..6849
                     /locus_tag="PLP95_02565"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RecQ family ATP-dependent DNA helicase"
                     /protein_id="HPB44712.1"
                     /translation="MTDVATDATRLLKQLAGPSARLRPAQLEAIEAVVGQRRRALVVQ
                     RTGFGKSAVYFIAAKLLRERGAGPALVVSPLLALMRDQVAAAERMGVRSATINSTNLE
                     EWADIEAQLGAGELDLLCISPERLNHPRFAREVLPRLAAGLGLLVIDEAHCISDWGHD
                     FRPDYRRIRDAIANLDPSTPVIATTATANERVSLDVAEQLGDDVLVLRGTLDRESLSL
                     GVTNLATQAERLAWLAQRMVQVEGTGIVYCLTVDQTQQVAEFLQREGLDARAYSGKTS
                     TEERLILEDDFKANRLKALVATSALGMGVDKADVTFVYHLGAPPSPIAYYQQVGRAGR
                     SVDHAEGWLLPGAEDRAIWEYFASVGLPPERLVQRVLEALGDATERGAGPVSVAALER
                     QVDLRRTRLETLLKILDVDGAVERTSQGWVLTGQPWVYDRERVERVEQARRDEADQMV
                     AYATAATCLMRFLREALDDPEAVDCGRCSVCTGSGDPVDLDEATVRRAVEFLRGVDVV
                     VDERKQWPRGNDESMPRGNIKPDRRAQEGRALCRVNDAGWWPLVSRCRELGEADDELI
                     RGVFAALKRWSWGVRPTWVTYVETEGVDPASGAAHLARSVAERIATAGKLQLAPTLTW
                     RGGVGTSSNSAFRATDVWNGLVLEHEPPSLAGPVLLILDEVDSRWTQTVAAYRLLEAG
                     SGPVLPFALILR"
BASE COUNT         1166 a         2126 c         2577 g         1279 t
ORIGIN      
        1 tgctgcgagc aacaacggga ggccaggaat gccaccctcg ttctgatacg tgagcagcga
       61 cccgatcaag atgaccagga aggtcgcggt gaacgtcacg atggtgttga gcccgatgta
      121 gccgagcgag tggccgaaga ttcgttcgaa cttgagggtg cacgtcggac actcgggtcg
      181 catcttgaag tacctgtgat gcgtattgcc atgcccacag gcgggacacc ggatgagcaa
      241 ccctcgcatg accattttga gattcgacac gccgagttgc tcgctcgttt gtgtcgggtg
      301 catggagttc atgtgcccat catcgccgcg cgttgtcagc gatgcctcat cgcagcggtg
      361 gttcgctcga ccacgggaaa ttgatccagt cgtcggtgcg cttccagacg tagtcgggcg
      421 acagcagcga ccgaggcttc tcgtagatca ccagggatcg gatctccgcg acgaccggtg
      481 cgcaggtgtc gacgacgagc ttgagggttt cgccggtgtc tgccacgtcg tcgacgatga
      541 gcaacttggt gtccgcgaga tccgcgaggt tcagcgctgg cggcaacatg acgggcacgt
      601 cgaggcgctc gtcgacatcg gagtagaact cgacgttgac cacgccgatg ttcttgcagc
      661 tcaacgcgta cgacacggcc ccaccgatga gcaggcctcc tcgggcgatc gcaacgatcc
      721 agtcgggttg atatccgctg tcgacgacct gctggctcac ctcacgaacc gcgacgccga
      781 aggtcgcgta gtcgaggtcc tcgcgtgggc cggtcgtgac tgcttccata ccgagatgtc
      841 cttctatggt gcgagacgcg gggctagccg atgcgaaccc ctgccgactg gaatgccgct
      901 cgaagactcc catattggtc gaacccgcag cgaagccgca cgagttcgag ttcggtggag
      961 acgaacgcgg ggccgtgagg ttcgctttgg ggcacggtcg tcgccacgac gagatgggcg
     1021 agttcgtgca gcaccgtgag cgcgttccat gagccgtcgc ggataaggat gatgtcgccg
     1081 cctgccacgg cgagcgagga gcgagccgac cgggagcgtc gttcgagggc cacgtcgagg
     1141 ggagcgtcgg gaaacgcgtc gaggtatccg ggtgagatca gcacggactc gacgaatgat
     1201 tcgacctcgc gccaacgacg taggcgcggt ccgatttggt cgatgacgct gtcttcggcg
     1261 tggtagacga ggaactgttg cgggtcgtcg gcgtcggggc gaccggtgtc gcgagatcgt
     1321 gtcacgggga ggctcttcgc ccggggaggg cggggcgtgc tccgagacga gtgtggccga
     1381 tgtcggcgcg gttgcccgcc gcggtgccgg cccgatgtcc ggaccgcgag gtacccgcgc
     1441 tgacccgtga cgtgcggatg ttcgggtatc ggcgccgaaa gtcggtgtgg acagcgtcgg
     1501 cgcggtcggc gagcacgaga tcggtcgaac ggctaccggc ggccccacgt tcgctcggac
     1561 cttggttggt ggacgggccg tcggcttggt tcgcgaccgc gtccatcgcc ccgcgacggt
     1621 cctggatcag gcggcggatc acggtgtcgg cgaacgcgga gatgaagctg cgtcgccagc
     1681 tcgacacctg gctggatgtc gccgatcgcg gctggtcggg ggagtggacc atcgcggtcg
     1741 agagttggac caacaagctg gtgatcaggg tctcgacgag ggtgaggtcg gatggaaagc
     1801 cgacgactga aatcacttcg ccgccttggc cccctcgtcg caatcgaaga gcttggcacc
     1861 cgaaggccga ggccacgccg gcgacgagaa tcgacttctg agcggtgaac gggcgcaaca
     1921 gcgtgaggtg gatctcatcg gggacctcac gatcagcctc gctttcgcca gaccacagca
     1981 tcgccgcatc gatcgcatag cgcgccatga gttcggtggc cttggcggtg aacgcctcgg
     2041 cctcggcttc gaaggtcgtg gcgtcggcct tggccaacaa cgcgcggatg gtgtcgagtc
     2101 gggcatcgga gtcgcggagt tgggccatgg gcaacattgt tgccgcgacc cccgacagtt
     2161 ggtgccgctg gcaccgtcct cagggtattt ggaacagatg gatgccggtg gccgttactc
     2221 tcgttcggcc gctcgcagcg atgacggccc gtgttgggcg actgtgtcgc gaaccacgag
     2281 agggtgccat ggctcgtcac atctggaagt tgctcggact cgccggagcg gcggttcttg
     2341 tcgtgaacct gctcggggtg aacccgctcg ggtgggtcgc cgatggcttg tgggcgagcg
     2401 acgatccgcc ggcggcggcg gacacgacgc tgttgtcgat caccaagaca caggatctgc
     2461 gagcggcgac cggcgagttc agcgtgccgg tctacttcgg gaccgagcag aacggtttct
     2521 tgaagaagat catcccggac gcgttcgatg ctgattcggg cattgcgctc taccagggaa
     2581 gcgtggatgc cttcatcgac ctcagcgggt tgaccgagga tgacctcgag atcgacgagg
     2641 cggctaacac gctcaccatc accgtgccgg atcccacgct gtcgaccccg cgtctcgatc
     2701 acgagaaatc gtcggtggtg tcgcaggatc gcggcgtgtt gacccgactg ggtgaggcgt
     2761 tctccgagtc tccgatggag gggcgggaca aactcgacga aacggcggtg aacgagctgg
     2821 cgaaggcggc ggcgcagagc aatctcctcg ataccgcgcg cacgaacggt gaggacttcc
     2881 tcgtcgcgct cggcgaacaa ctcgggttcg atcgcgtgat catcgtgttc gagaagccga
     2941 gcaacccctg agttctggct tggcgtcgcg cttggcgagc gcggaacccg tcagcgattc
     3001 caggcggtga cgagcgcgtc gatctgagcg cggcgatacg ccttggcgac cggtgcccac
     3061 cgttcgaggt cgaagccgtg ataggccccc tcgaccacca tgagttccac gtccgagccg
     3121 gcttctcgca atcggcacgc gtagtcgagg tcctcgtcat ggaagaggtc gttggtgccg
     3181 accccgaccc acgcgctcgg caaaccggtc aggtcttcgc agcgcccggg caccgcccag
     3241 gcggagagct cctctggacg tcgaccaccg aggtaggagt cccaaccgag ccgattgctc
     3301 gtcgtgcccc acagacgcag cgtcgagggg gcgatgtcgt ctcggtcgga gctgcggtcg
     3361 tcgagcatcg gatacgacag cacctggaaa cacggctgga cctccccgcg ttctctcgcc
     3421 aggatcgcga gcgccgcggc gaggcctcct ccggcgctct cgccgccgat ggcgattcgg
     3481 ttcgggtcga cgtcgggctg acgcgacagc catacgagcg cgtcccaaca atcctcgagc
     3541 ggagcgggaa acgggtgctg cggcgcgagg cggtattcga ccgacgcaca cacggcaccg
     3601 tggcggcggg cgatgtggcg aagcacaccg tcggccatcg acgccgaacc gatgatgagt
     3661 cctccgccgt gcatccacaa cagcgccgga cggccggtgg atggcaacgt cggatcggtc
     3721 gggcggaaaa cccgaacgct cgaggttgcc gagcatcgtt cgatcttccc gccgatcggc
     3781 ggtcgtcggc cggcgagaca ggtcagccgt tgaatggtcg gcatcgtgcg ctgggtgatc
     3841 ggcgaacgtg gaacgaatcg agccagtcgg agttcagggt ggaagcggtc ggcgctcaca
     3901 tcgcctcgat tcggatcagt cggcgggctt gaacgacttg gtgtcgacga gtcgttggat
     3961 ccggtcgctg aggttcggcg cggcgacggc gagcgtcgcg acggcacgaa gttcgatcgg
     4021 cgacacgaag ctttcggcgg tattgcgttg aatcgcccgc accacgcccg cggcgacgtc
     4081 gtcgggcgat ttcatgcgaa ccaccctggg gagctgtacg ccattgtcgg cgaacatgcc
     4141 ggcggtggcg atgaaactcg gctcgacgat gctcaccccg atgccgtgct cggcgacatc
     4201 gtgacgcatc gccaacgcga agccgcgcag gccgaatttc gtcgagttgt acaggcgcgt
     4261 gttcggagtc ggtgcgaggc ccgacagtga gccgacgaag acgatgtggc ccgttcggcc
     4321 ctcgtcgatg aggcgttgcg cgaactcgac ggagaggtga atcggggagc gcaggttggt
     4381 gtcgatgacg cggtcgatgt tttcggctgt ctcgctgcgg atgccgacgt cgttgccggt
     4441 gccggcattg gcgatcagga tgtccgcagc gctcgccgcc tcgatgagcg tggcgatgga
     4501 tgtcggatcc gtgaggtcgg cgacgacgac ggacgcgtcg agttcgtcgg cgagttggtt
     4561 gagttcggcc tctcgacgac ccgtcagaat gagcgtggcc ccttcgcgta cgagacgcct
     4621 ggcgatggcg tggccgaggc caccgctggc accggtgagc agcacggtgg acccggagat
     4681 gttcatcgcg ccacggtagt gcgcgccgct ccacagcggt tgggcgtctg gtaactgtca
     4741 ggggtccgcg acagggtgga ggccatgacc gacgtggcga ccgatgcgac ccgactgttg
     4801 aaacaactcg ctgggccgag cgcgcggctt cggcctgcac agcttgaggc gatcgaggcc
     4861 gtggtcgggc agcggcggcg ggcgttggtg gtgcagcgga ccgggttcgg gaaatcggcg
     4921 gtgtacttca tcgccgccaa gttgttgcgt gaacgcggcg ccggcccggc gctggtggtg
     4981 tcaccgctgt tggcgttgat gcgcgatcag gtagcggcgg ccgaacggat gggcgtgcgt
     5041 tcggcgacga tcaactccac caacctcgaa gagtgggccg acatcgaagc acaactcggc
     5101 gctggcgaac tcgacctgtt gtgcatcagc cccgagcggc tgaaccatcc gcggttcgct
     5161 cgtgaggtgt tgccgcgtct cgctgcgggg ctcgggttgt tggtcatcga cgaagcgcac
     5221 tgcatttcgg attggggtca cgatttccgc cccgactacc gccgcatccg cgatgcgatc
     5281 gccaacctcg acccgtcgac tccggtgatc gcgacgacgg cgacggcgaa cgaacgggtg
     5341 agcctcgacg ttgccgagca actcggcgac gatgtcttgg tccttcgcgg cacgctcgat
     5401 cgcgagtcgt tgtcgctcgg cgtgacgaac ctggcgaccc aggccgaacg cctggcgtgg
     5461 ttggcgcaac gcatggtgca ggtggagggg accggcatcg tctattgctt aaccgtcgat
     5521 cagactcagc aggtcgccga gttcctgcag agggagggtc tcgatgcccg ggcgtattcg
     5581 ggcaagacct cgaccgagga gcgcctcatt ctcgaggacg acttcaaagc gaaccgcctg
     5641 aaggccctcg tggcgaccag cgcgctcggt atgggggtcg acaaggcgga cgtgacgttc
     5701 gtgtaccacc tgggggcgcc accgtcgccg atcgcgtact accaacaggt cggccgtgcc
     5761 ggtcgctcgg tcgatcacgc cgaaggctgg ttgttgccgg gggctgagga ccgcgcgatc
     5821 tgggagtact tcgcctcggt cgggttgccc cccgaacgcc tggtacagcg ggtgcttgag
     5881 gcgctcggcg atgccacgga gcgtggtgcc ggacccgtga gcgttgccgc gctcgagcga
     5941 caggtcgacc ttcggcgcac ccggctcgag accctgctga agatcctcga tgtcgacgga
     6001 gcggtcgagc ggacctctca ggggtgggtg ctcaccggtc agccctgggt atatgaccgc
     6061 gagcgcgtcg agcgggtgga acaggcgcgt cgcgacgagg ccgatcagat ggtcgcctat
     6121 gcgacggcgg cgacgtgtct gatgcggttc cttcgtgagg cgctcgacga ccccgaggcc
     6181 gtcgactgcg ggaggtgttc ggtgtgcacc ggctcgggtg acccggtgga cctcgacgag
     6241 gcgaccgttc gtcgtgccgt cgagttcctc cgcggcgtcg acgtcgtggt ggacgagcgc
     6301 aagcaatggc cgaggggtaa cgacgagtcg atgccccgcg gcaacatcaa acccgaccgc
     6361 cgggcgcagg agggacgtgc gctgtgccgg gtaaacgacg ccgggtggtg gccgctcgtg
     6421 tcccgctgtc gcgagttggg tgaggccgat gacgagttga ttcggggggt gttcgcagcg
     6481 ctgaagcggt ggtcgtgggg cgtacgcccg acatgggtga cctacgtcga gacggagggg
     6541 gtcgacccag cgtcgggcgc cgcacacctg gcacgttcgg tcgccgagcg gattgccacc
     6601 gctggcaagt tgcagttagc tccgacgttg acgtggcgtg ggggtgttgg gacgtcgtcg
     6661 aactcggcgt ttcgagcgac cgacgtgtgg aacggcctcg tgttggaaca cgaaccgccg
     6721 tcgctggcgg ggccggtgct gctcatcctc gacgaggtcg atagccggtg gacccagacc
     6781 gtggcggcct atcgactgct tgaggccggg tcggggccgg tgttgccctt tgcgttgata
     6841 ctgcgctgag tgcgacaaca cgtccggcta tgagcacgag cggtcgccgt gttgaagcga
     6901 gtcggcgcca tgcgttgcgt gacatcggtt cgaagggggt gattcgtggc ctcacggcaa
     6961 cgaccttggt ggaggtgaca tatggcggac accctcgtcc ggctttcgtg gggtctcggt
     7021 accgtatgtg agtatcagtt catattcggt gggtgatggt cgttctactg gttcgggttg
     7081 tcgtggtgtc ctttggttga ggttggcacg ctgcctgctg tcggtggtga tgttgtctgc
     7141 ggtgctcg
//
LOCUS       DAOOMA010000053         7131 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_12467_length_7131_cov_4.033493, whole genome shotgun sequence.
ACCESSION   DAOOMA010000053 DAOOMA010000000
VERSION     DAOOMA010000053.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 7131)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7131)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7131
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12467_length_7131_cov_4.033493"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(48..2324)
                     /locus_tag="PLP95_02570"
     CDS             complement(48..2324)
                     /locus_tag="PLP95_02570"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase"
                     /protein_id="HPB44713.1"
                     /translation="MAVSATTPIELVNSVYATLEQRLSAARERLGRDLTLAEKILFNH
                     LDPSDTAEPERGVSYNDLRPDRVAMQDATAQMALLQFMTAGLPQVAVPSTVHCDHLIQ
                     AKEGAKVDLLAAETGNREVYDFLESVSAKYGIGFWGPGSGIIHQVVLEQYAFPGAMMV
                     GTDSHTPNAGGLGMIAIGVGGADAVDVMTGFPFNIRWPKLIGVHLTGSLNGWSAPKDI
                     ILKVAEILTVKGGTGAIVEYFGEGANSISCTGKATICNMGAEIGATTSLFGYDAAMGR
                     YLASTGRGAIATAADAVAEHLRADDSVLANPAEYYDQVIEIDLSTLQPHINGPHTPDL
                     AREVKDLGSEAKLNGWPLEISSALVGSCTDSSYEDITRAASILREAAANGLKVKTPLL
                     ITPGSEQVRATIERDGLLGTFEAAGATVLANACGPCIGQWSRPESVTSEPNTIVTSYN
                     RNFPKRNDGSANTLAFVTSPETVVALALAGSVDFDPINDTLTNDAGEQVKLSVPVGQE
                     LPEAGFTPGENTFIAPPEDASGLEVKVSPTSDRLQLLEPFAAWDGKDFDDLPILLKAK
                     GKCTTDHISMAGPWLKYRSHLENISGNLFLGAVNAFTGAVGEGKDQLDGSTRSFPDIA
                     KHYHEAGQPWIAVGDENWGEGSSREHAAMEPRFRGAKAILVRSFARIHEANLKKQGVL
                     PLTFADPTVYDQIGEDDRISIVGLADLAPGRPVDLVLTKPDGTTLNFQANQTMSPEHI
                     EWFKAGSALNIIRANAAK"
     gene            2494..3369
                     /locus_tag="PLP95_02575"
     CDS             2494..3369
                     /locus_tag="PLP95_02575"
                     /inference="COORDINATES: protein motif:HMM:NF019322.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HPB44714.1"
                     /translation="MDLPNEVDVTASSPKRRIRYQSIAEDVRRRIVDGELQPGVLLPS
                     ESELSGRYSVSRVTVRRALEALRGDGLIDSRQGFGWFVATDPLRQELRELGTMESQLR
                     AVGMSSERRILSFSFVTAPPAVAQTLRDDEVLEVRRLNLADGQPFAVVTVWCPARLGA
                     QLSRSDVERSSFLEQLPVEIGGASQTIGARAASEETAKLLDIPVGSPLLVADRVTRSA
                     DGAAVLISRHEFAAHRTELWWSCPPRSPAGWAPRVFGSWSSGGQAGGPAAGQDGARLR
                     AGRCSTGFRGGRRRC"
     gene            complement(3344..3682)
                     /locus_tag="PLP95_02580"
     CDS             complement(3344..3682)
                     /locus_tag="PLP95_02580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp family type IVb pilin"
                     /protein_id="HPB44715.1"
                     /translation="MGRTKRFATAAVGRAAFRGKTFDPACLIGLQRQRSRSDTQVMEP
                     QELIDNTTEALDSTDGSSHGLAASRSERGANLVEYALLLALIAIVCVAGVAALGETLP
                     AGFSSVSGHL"
     gene            4072..5160
                     /locus_tag="PLP95_02585"
     CDS             4072..5160
                     /locus_tag="PLP95_02585"
                     /inference="COORDINATES: protein motif:HMM:NF012335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HPB44716.1"
                     /translation="MRAAVMNGQRISVEDVDDPTPGPGQVVIAPEATGICGSDLHMRE
                     AYASFAQAGLEVEPIIPGHEFAGRIVALGSDVDAPGGGTGTGTGSGTGDQVLRVGDFV
                     TAIPFTHGPDGPEGIGLSPRFGGGLAEFVLADATSTFVLPESIHPDRAGAVGALAEPL
                     AVGLRALSKAAPQGPIVVVGTGPIGLMVIAAAVLQGRGPVIAVDPSEQRRDVARRVGA
                     HSAHAPGASLVELLGTVGYRPSTMSPLLREDPEPATIVECVGRPEVIETLLTQAPAHS
                     RVVLAGACIHSLEMPVLQLTLAEVAVETSFAYRPADFVTSAQLLGTHPEIFSSMITAQ
                     LPLAETEHAFALLENEPEQVKILINPNA"
     gene            5217..6038
                     /locus_tag="PLP95_02590"
     CDS             5217..6038
                     /locus_tag="PLP95_02590"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HPB44717.1"
                     /translation="MSESATPASVPMTFPDRFAGKVALVTGGASGIGRATVQRFVAEG
                     ARVVVGDLADDALAAVSDEFGEAVRTQRCDVTNEDDVAALVALARTEFGGLDVVCANA
                     GTGGFGLITDMDLDEWNRVVDVNLTGPFLTIKHAARAMNDGGAIVITASLNAVQPAAG
                     MSPYCASKAGVAMLARVAAMELGPRRIRVNAIAPGLVSTGLTAPLMGIDAVRDGYVEN
                     TPVGRYADPREIAGLIGYLASDEASFVSGALHLIDGGANTMRYPDLVGALNSLEV"
     gene            6063..7043
                     /locus_tag="PLP95_02595"
     CDS             6063..7043
                     /locus_tag="PLP95_02595"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HPB44718.1"
                     /translation="MVEHQHLIVSNGDIDIAVDLATSGEIASRGTVVCVAGWPELGRS
                     WRHQQRHLVERGYRVATVDVRGYGASSAPPEVERYTLRELSGDIAAVAEAVSDDPVVV
                     LCHDWGAPIGYHTAIRHPDSVAAVIGLSVPHMAPNGSSPIEMLDLLYADRFFYMLYFA
                     RPGVAEADFGRDLRGSLKRCYWALSGEAPAGAWTPDAPRDASLLDLLPPCPDGPLSFL
                     PDDELDALVSAFERTGMTGAFNRYRASALDAAADLDIVGARVEQPSCFIAGALDPVRN
                     MVPGVDLYAEAGADCSEFRGTTLIPGAGHWVHQEAIAATNAAIDAFVDSL"
BASE COUNT         1067 a         2292 c         2544 g         1228 t
ORIGIN      
        1 ggggttcgcg tacggtcgtg agcgcgccca ggcccaggcc caggcggcta cttcgcggcg
       61 ttcgcccgga tgatgttgag tgccgagccg gccttgaacc actcgatgtg ttcggggctc
      121 atcgtctggt tcgcctggaa gttcagcgtc gtgccgtcgg gcttcgtgag cacgaggtcg
      181 accggcctgc cgggagcgag gtcggccaga ccgacgatgg agatgcggtc gtcctcgccg
      241 atctggtcat agacggtcgg atccgcgaag gtcagcggca acacgccctg cttcttgagg
      301 ttggcctcgt ggatgcgagc gaagctgcgc accaggatcg ccttggcgcc gcggaatcgc
      361 ggctccatcg cggcgtgttc gcgcgacgag ccctcacccc agttctcgtc gcccaccgcg
      421 atccacggct ggccggcctc gtggtagtgc ttggcgatgt cggggaacga ccgcgtcgag
      481 ccgtcgagct gatccttgcc ctcgccgacc gcaccggtga aggcgttgac cgctccgagg
      541 aacaggttgc ccgagatgtt ttcgaggtgg ctgcggtact tgagccacgg accggccatc
      601 gagatgtggt cggtggtgca cttgcccttg gccttcagca ggatcggcag gtcgtcgaag
      661 tccttgccgt cccaggcagc gaagggctcg aggagttgca ggcgatccga ggtcggcgac
      721 accttgacct ccaggccgct cgcatcctcg ggcggggcga tgaaggtgtt ctcacccggg
      781 gtgaagccgg cttcggggag ctcctggccg accggcaccg acagcttcac ctgttcgccg
      841 gcgtcgttgg tgagcgtgtc gttgatgggg tcgaagtcga ccgatcctgc gagcgcgagg
      901 gcgaccacgg tttcggggga cgtcacgaat gcgagcgtgt tggccgagcc gtcgttgcgc
      961 ttcgggaagt tgcggttgta ggaggtgacg atcgtgttcg gctcgctggt caccgactcg
     1021 ggacggctcc actgaccgat gcacggaccg caggcgttgg ccagcaccgt cgccccggct
     1081 gcctcgaagg tgccgagcag gccgtcgcgt tcgatcgtgg cgcgcacctg ttccgagccg
     1141 ggggtgatga gcagcggcgt cttgaccttc aggccgttcg ccgcggcctc cctgaggatg
     1201 gaagcggcac gggtgatgtc ctcataggac gagtccgtgc acgacccgac gagtgccgag
     1261 ctgatctcga gtggccagcc gttcagcttc gcctcgctgc cgaggtcttt gacctcccgg
     1321 gcaaggtccg gggtgtgcgg gccgttgatg tggggctgca gggtcgacag gtcgatctcg
     1381 atgacctggt cgtagtactc ggccgggttg gccaatacgg agtcgtcggc ccggaggtgt
     1441 tcggcgaccg catcggcggc ggtggcgatc gccccccggc cggtcgaggc gaggtagcgg
     1501 cccatcgctg cgtcgtagcc gaacagcgag gtggtggcgc cgatctccgc gcccatgttg
     1561 caaatggtgg ccttgccggt gcagctgatg ctgttcgccc cttcgccgaa gtactcgacg
     1621 atggcgccgg tgcccccctt cacggtgagg atctcggcga ccttcaagat gatgtccttc
     1681 ggcgccgacc agccgttgag gctgccggtg aggtggacgc cgatcagctt gggccagcgg
     1741 atgttgaacg gaaacccggt catgacatcg acggcgtcgg ccccgcccac accgatggcg
     1801 atcatgccga ggccgccggc gttgggggtg tgggagtcgg tgccgaccat catggcgccg
     1861 gggaaggcgt actgttcgag cacgacctgg tggatgatgc ccgaaccggg tccccagaag
     1921 ccgattccat acttggcgga caccgactcg aggaagtcgt agacctcgcg gttgccggtt
     1981 tcggcggcga gcaggtcgac cttggctccc tccttggcct ggatgaggtg gtcgcagtgc
     2041 accgtggagg gcacggcgac ctgcgggagg ccggcggtca tgaactgcag cagcgccatc
     2101 tgggcggtgg cgtcctgcat ggccacgcgg tcggggcgta gatcgttgta ggagacgccg
     2161 cgttcgggtt cggcggtgtc gctggggtcg aggtggttga agagaatctt ttcggcgagg
     2221 gtcaggtcgc gtccgagacg ctcgcgggcg gcgctcaggc gctgctcgag tgttgcgtag
     2281 acgctgttga cgagctcaat gggggtggtc gctgagacag ccatgtgcgc tcctaggtgt
     2341 tggggtgtgt tcgtgatctg gacggtaccc gttgggcggg tcgaccgggt ttcggaatcg
     2401 gctcggcgcc ggtaccgtgt ctccactaga tacaagtaga gtacaagtgt cgaccgcaag
     2461 tatacaagta ccagacaagt ggggtcggag cgcatggacc tgccgaacga ggtggacgtc
     2521 acggccagca gcccgaaacg tcggatccgc tatcaatcga tcgccgagga cgtgcgacgt
     2581 cgaatcgtcg atggcgaatt gcagccggga gtgttgttgc cctccgaatc ggagctctcg
     2641 ggtcgatatt cggtgagtcg ggtgacggtt cgtcgggcgc tcgaggcgct gcggggcgat
     2701 ggcctcatcg attcgcgcca ggggttcgga tggttcgtgg cgaccgaccc gctccgccag
     2761 gagttgcgcg agttgggcac gatggaatcg cagctccgtg ccgttggcat gtcgagtgaa
     2821 cgtcggatcc tgtcgttctc cttcgtcacc gcgccgccgg cggtcgctca gaccctgcgg
     2881 gatgacgagg tgctcgaggt gcgccgactc aacctggcgg acggccaacc gttcgccgtg
     2941 gtcacggtgt ggtgcccggc ccgcctcgga gcgcagctgt cacgctccga tgtcgaacgg
     3001 tcgagcttcc tggaacagct ccccgtcgag atcggtgggg cgagccagac gatcggcgcc
     3061 cgcgccgcaa gcgaggagac cgccaagctg ctcgatatcc cggtcgggtc gccgttgctc
     3121 gtcgcagacc gggtgacgcg atccgccgac ggcgcggcgg tgctgatcag ccgtcacgag
     3181 ttcgccgcgc accgcaccga gttgtggtgg agttgcccgc cgcggtcgcc agcgggttgg
     3241 gccccgcggg tcttcgggtc ctggagtagc ggcgggcagg caggcgggcc ggcggccggg
     3301 caggacggtg ctcggctgcg ggcgggacgg tgctcgaccg ggttcagagg tggccggaga
     3361 cgctgctgaa tccagcgggg agggtttctc cgagtgcggc aaccccggcg acgcacacga
     3421 tggcgatcaa cgccagcagc agtgcgtatt cgacgaggtt ggcgccgcgt tcggatcgag
     3481 acgctgccag gccgtgggag gaaccatcgg tcgagtccag cgcttcggtc gtgttgtcga
     3541 tgagctcctg gggttccatg acctgggtat cggagcgtga ccgttggcgc tgaagcccga
     3601 tgaggcaagc agggtcgaac gtcttaccgc gaaacgccgc ccggcccacc gcggccgtcg
     3661 caaaccgctt cgttcgcccc aaaacgccgt tctccgataa ccagtggtcg tgatcacgac
     3721 cactggttat cggagaacag gcgggggggt gtggggtgtc agcccttgcg gcggccgaac
     3781 caggaccgcg acgttgtcgc ggcgcttgag gcgttggacg cgcattggca tcggtcggcc
     3841 ggggcgacat cgccgaggac ctgttcgacg tgacgtccgc atccggcgaa ggtgggcttg
     3901 tggcaggtgg ggcattgaac tcgttgacac atacccccca gggtatacga aattcaaccg
     3961 attgcaagtc atcgcgcgga gagcgcttgg gctacggtcg gggctgagtg gaccgccgaa
     4021 gggaatcgcc gcagggggtg gcgatcgatc ggttcgccga ggggagtcca cgtgcgcgca
     4081 gcggtcatga acgggcaacg gatctcagtc gaggacgtgg acgacccgac accaggtccg
     4141 ggccaggtgg tcatcgcccc cgaagcgacc ggaatctgcg gcagcgacct gcacatgcgc
     4201 gaggcctatg cctccttcgc ccaggcgggc ctcgaggtcg agccgatcat ccccggccac
     4261 gagttcgccg ggcgcatcgt cgccctcgga tccgacgtcg acgctcctgg aggcggaacc
     4321 ggaaccggaa ccggttccgg aaccggcgac caggtactgc gggtcggcga cttcgtcacc
     4381 gccattccct tcacccacgg tcccgatggc ccggagggga tcggcctgtc gccgcgtttc
     4441 ggcggcgggc tggccgagtt cgtcctcgcc gacgccacat ccaccttcgt gctcccggag
     4501 tcgattcacc ccgatcgggc gggcgccgtc ggggcgctgg ccgaacccct ggccgtcgga
     4561 cttcgggcgc tgtcgaaggc ggcgccgcaa ggtccgatcg tcgtcgtcgg caccgggccg
     4621 atcggattga tggtgatcgc ggcggcggtc ctgcaagggc gcggtccggt gatcgccgtc
     4681 gacccgtccg aacaacgtcg cgacgtcgcc cgccgcgtcg gggcccactc ggctcatgct
     4741 cccggagcgt cgctggtcga gttgctgggc accgtcggat atcggccgag caccatgtcg
     4801 ccgttgctgc gcgaggatcc cgagccggcc acgatcgtgg aatgcgtcgg caggccggag
     4861 gtcatcgaga cgctgctgac ccaagccccg gcccacagcc gagtggtcct cgccggggcc
     4921 tgcattcact cactcgagat gcccgtgttg cagctcaccc tggccgaggt cgcggtcgag
     4981 acgagcttcg cctaccgccc cgccgacttc gtcacctcgg cgcagctgct gggaacccac
     5041 ccggagattt tctcgtcgat gatcaccgca cagctcccgt tggcggagac cgaacacgcc
     5101 tttgcgctcc tggagaacga acccgaacag gtgaagattc tcatcaaccc gaacgcctga
     5161 acccggaacc agaacctgaa cccggaaccc gaaacccgga acctgcgagg aaccccatgt
     5221 ccgaatccgc cacacctgca tctgtcccca tgaccttccc cgaccgcttc gccggaaagg
     5281 tcgcgctcgt caccggaggc gcgtccggaa ttggacgagc cacggttcag cgattcgtcg
     5341 ccgagggcgc ccgcgtggtc gtcggcgacc tcgccgacga tgcgctcgcg gcggtgagcg
     5401 acgagttcgg cgaggccgtt cgaacgcagc ggtgcgacgt cacgaacgag gacgacgtcg
     5461 ccgcgctcgt cgcgctggcc cggaccgagt tcggcgggct cgacgtcgtg tgcgcgaatg
     5521 ccggaaccgg cggattcggg ctgatcaccg acatggacct cgacgagtgg aaccgggtgg
     5581 tcgacgtcaa cctcaccgga ccgttcctca cgatcaaaca cgcagcgcgg gcgatgaacg
     5641 acggcggagc gatcgtcatc accgcgagcc tcaacgcggt gcagccagcc gccggcatgt
     5701 cgccgtactg cgcctcgaag gcgggcgtgg cgatgctggc ccgggtggcg gcgatggagc
     5761 ttggcccgcg gcgaatccgc gtgaacgcga tcgcaccggg tctggtgagc accggcctca
     5821 ccgctccgct gatgggcatc gacgccgtgc gcgacggata cgtcgagaac acaccggtcg
     5881 gccgctacgc cgacccccgc gagatcgccg ggctcatcgg atatcttgcc tcggacgagg
     5941 cctcattcgt gtcgggcgct ctgcatctca tcgacggggg agccaacacg atgcgctacc
     6001 ccgacctcgt gggtgcgttg aactcgctcg aagtctgacc tcgaaaggtc gggaggatcc
     6061 ccgtggttga gcaccagcac ctcatcgtgt cgaacggcga catcgacatc gccgtcgacc
     6121 ttgcgacgag cggcgagatc gcgagtcgtg gcaccgtcgt gtgtgtcgcc gggtggccgg
     6181 agctcggccg ttcgtggcgg catcagcagc gtcatctcgt cgaacggggg taccgggtcg
     6241 cgacggtcga cgtgcggggg tacggcgcca gctctgcgcc gcccgaggtg gagcggtaca
     6301 ccctgcgcga gctgtcgggg gacatcgctg cggtcgccga ggcggtctcg gacgatcctg
     6361 tcgtcgtgct gtgtcacgac tggggtgcac cgatcggcta ccacaccgcc attcgtcatc
     6421 cggatagcgt cgccgcagtc atcgggctga gcgtgccgca catggcgccg aacggctcgt
     6481 cgccgatcga gatgctcgat ctgttgtacg ccgatcggtt cttctacatg ctctacttcg
     6541 ctcgtccggg ggtggccgag gccgacttcg gacgcgacct gcgaggctcg ctcaagcggt
     6601 gttactgggc gctgtcgggc gaggccccgg ctggtgcgtg gacccccgat gcgccacgtg
     6661 atgcgtcgct gctcgacctg ttgccgccgt gcccggacgg gccgctctcg ttcctgcccg
     6721 acgatgaact cgacgcgctc gtctcggcct tcgagcgcac tggcatgacc ggagcgttca
     6781 accgctaccg cgcctcggcg ctcgacgctg cggccgatct cgacatcgtc ggcgcccggg
     6841 tggaacagcc gagctgtttc atcgccggtg cgctcgatcc cgtgcgcaac atggtgcccg
     6901 gcgtcgacct gtatgccgag gccggggccg attgctccga gtttcggggc accaccctca
     6961 ttccgggcgc tgggcattgg gtgcaccagg aggcgatcgc ggccaccaac gccgccatcg
     7021 acgcgttcgt cgattccctg taggggatcg ctgacggtcg tacgcgccca gtcctgatcg
     7081 cgcccgggcc cagttcgagc ccgggcccag ttcgagcccg ggcccagttc g
//
LOCUS       DAOOMA010000054         7083 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_12573_length_7083_cov_4.060472, whole genome shotgun sequence.
ACCESSION   DAOOMA010000054 DAOOMA010000000
VERSION     DAOOMA010000054.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 7083)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7083)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7083
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12573_length_7083_cov_4.060472"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..905
                     /locus_tag="PLP95_02600"
     CDS             <1..905
                     /locus_tag="PLP95_02600"
                     /inference="COORDINATES: protein motif:HMM:TIGR01494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HPB44719.1"
                     /translation="LVGVAAADGVDRSELLAVAGAIEAASEHPIAKAVASGARAELGS
                     LEPVGDFSALEGRGVRGTVGGRSVMVGREALLTAEGFHVGDDVREAKAESERRGETAV
                     VVAWDGEARGVVSVADAIKDTSAEAIRRLRGLGLEPVLLTGDNRSVAEEVAAQVGIDN
                     VIAEVLPSEKVDVVARLQSEGKTVAMVGDGVNDAPALAQADLGLSMGTGTDVAIEASD
                     ITLVAGDLRAAVDAIRLSRRTLSTIKGNLFWAFAYNVAAIPLAAFGLLNPMLAGAAMA
                     LSSVFVVSNSLRLRRFASISTERG"
     gene            902..1255
                     /locus_tag="PLP95_02605"
     CDS             902..1255
                     /locus_tag="PLP95_02605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HPB44720.1"
                     /translation="MSPADFDSPGVGDQHLPEPVCHNATDPGYMADKAKYLARIRRIE
                     GQVRGVHRMVDEEQYCIDILTQIAALRSAMQSLAVELLGDHLSHCVADAMQSGGEDAQ
                     AKIAEAKAAVARLAK"
     gene            1431..3287
                     /gene="ilvD"
                     /locus_tag="PLP95_02610"
     CDS             1431..3287
                     /gene="ilvD"
                     /locus_tag="PLP95_02610"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HPB44721.1"
                     /translation="MPELRSRTVTHGRNMAGARSLLRAAGVDGNDFGKPIIAVANSFT
                     EFVPGHTHLAPVGRIVSAAVKAAGGIPREFNTIAVDDGIAMGHGGMLYSLPSRDLIAD
                     SVEYMVEAHCADALVCISNCDKITPGMLNAALRLNIPTVFVSGGPMEGGEAKLVNGTV
                     RKGLNLITVIAEAVNDEVSDEDINRLEESACPTCGSCSGMFTANSMNCLTEALGLSLP
                     GNGSTLATHTARRDLYEAAGALVVELAHRYYDNDDASVLPRNIATYDAFENAMSLDIA
                     MGGSSNTVLHLLAAANEAGVPFTMADIDRLSRTVPCLSKIAPNGTYLMEDVHRAGGVP
                     ALLGELDRAGLLRHDVSCVHSVSLKQWLADWDIRGGAATDKAVELFHAAPGCVRSAEA
                     FSQSERWHSLDLDGENGCIRSVDHAYSAEGGLATLFGNIAEDGCIVKTAGVDESIFVF
                     SGPAGVFESQDDAVEAILKDEIHEGDVVVIRYEGPRGGPGMQEMLYPTSYLKGKGLGA
                     KCALITDGRFSGGTSGLSIGHISPEAAAGGAIALVRNGDRINIDIPNRSIELDVPAAE
                     LAERRGELEADNGYKPVARDRKVSAALKAYAAMASSADRGGVRDLSIIGIVD"
     gene            complement(3317..3835)
                     /locus_tag="PLP95_02615"
     CDS             complement(3317..3835)
                     /locus_tag="PLP95_02615"
                     /inference="COORDINATES: protein motif:HMM:NF013756.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase"
                     /protein_id="HPB44722.1"
                     /translation="MSGLPGFDAVNRQLDAAVVIAVVTTPTGDRSGCLVGFHSQVGIE
                     PLRYCVWISRENHTFGLLADASELSVHPLTDADREVATLFATRSGDDIDKWETMAHAG
                     LALPEAHVSGPIVDLLDHPDLDHVGVVLAPSHAHAAGGTDAADGSDGDTTNGSTLRVS
                     DVSDLAAGHEVD"
     gene            4052..4711
                     /locus_tag="PLP95_02620"
     CDS             4052..4711
                     /locus_tag="PLP95_02620"
                     /inference="COORDINATES: protein motif:HMM:NF037625.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HPB44723.1"
                     /translation="MTTTGLRERKKRETFVRIVTAARELVSERGIAGVTVDDIAEAAG
                     ISTRTFFNYFSSKNEAVIGSDPQGFEAAASALRNRPSDERPARALWSVITGEGDDAML
                     QRWMLRNELVRRHPELLPRHLESFDLATRALTEAVADRMDVDQGRDPRPAFVVATTIA
                     ALRSVMAWWQASDRSTDLHVHLATTLTTLEMLDLDAFRPDPVDPVDPVDPVSARRERI
                     S"
     gene            4708..6279
                     /locus_tag="PLP95_02625"
     CDS             4708..6279
                     /locus_tag="PLP95_02625"
                     /inference="COORDINATES: protein motif:HMM:NF018332.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MDR family MFS transporter"
                     /protein_id="HPB44724.1"
                     /translation="MTSTVPRQELTASGYTHRQVLVIFSGLMLGMFLAALDQTIVATA
                     LPEIVGDLGGASHLAWVVTAYMLTVSISTPLYGKLGDLYGRKRLFQFAIVTFLVGSML
                     CGVSQSMSQLIGFRALQGLGAGGLIVLAQALIAEVVTARERGRYQGYFGALFGVSSVA
                     GPLLGGFLTDNLSWRWVFYVNLPVGLVALVVTALKLPANGAPRSKPRIDYLGAALLAA
                     SVTCIVLVTTWGGEQYAWGSSLILSLIAVSVVLLGAFIAVERRAPEPVLPLHLFNLRT
                     FAVSSAVAFIVGVGMYGTISYLPYFMQTVNGASATDSGLVLLPLMLAMLLASMVAGQS
                     ISRTGRYRIFPVSGMVIAAVAMFLLSTMGGDTSKTLVTGYMALLGVGLGFCMQTLILA
                     VQNEVETKDLGVGTSAVNFFRSMGGSLGVAVFGAMFNAQFARNLPSFTHQLSEAERAA
                     LRLADPAEYAHYVYRFADSITSIFGWAAVVLAIGFGLSLMLRDTTFGTGAIHGSGDHD
                     GSDGTRTIGDRVVAQ"
     gene            6349..>7083
                     /locus_tag="PLP95_02630"
     CDS             6349..>7083
                     /locus_tag="PLP95_02630"
                     /EC_number="3.8.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="haloalkane dehalogenase"
                     /protein_id="HPB44725.1"
                     /translation="MNSVRTPDDRFVGLPGFDFEPRYHEVGDGLRMHYLDEGPRDGRV
                     VVLLHGEPSWSYLYRTMIPVLTAAGCRCVVPDLIGFGRSDKPTEQSDYTYARHIAWTT
                     ELLVDHLDVLDAVFFGQDWGSLVGLRVVTAHPDRFAAIAIGNGGLPNGDMAISDALRA
                     WREFAATADEFPVGAIINGGCTTDLSAEVIAAYDAPFPDDSFKAGARIFPSLIPVSPD
                     DVEARNQKAAWKVLEGWAKPFLCVYSD"
BASE COUNT         1119 a         2281 c         2404 g         1279 t
ORIGIN      
        1 cccttgtcgg cgtcgcggcc gcggacggcg tggaccgcag cgaactgctc gcggtggcgg
       61 gggcgatcga ggccgcctcg gaacacccca tcgccaaggc ggtcgcttcg ggggcgcggg
      121 ctgaactcgg atcgctcgag ccggtggggg acttctcggc gcttgagggg cgcggtgttc
      181 gcgggacggt gggcggtcgg tcggtgatgg tcggtcgcga ggcgttgctc accgccgagg
      241 gattccacgt gggcgacgac gtgcgcgagg cgaaggccga atctgaacgg cgtggtgaaa
      301 cggcggtcgt ggtcgcctgg gacggcgagg cccgcggtgt ggtgtcggtg gccgacgcga
      361 tcaaggacac cagtgccgaa gccatccgcc gtctgcgcgg gctcggcctc gaaccggtgt
      421 tgttgaccgg cgacaatcga agcgttgccg aagaggtggc ggcgcaggtt ggtatcgaca
      481 acgtcatcgc cgaggtgttg ccgagcgaaa aggtggatgt cgtggcccgg ttgcagtcgg
      541 aaggcaagac cgtggcgatg gtcggcgacg gagtcaacga cgcgccggcg ctggcgcagg
      601 ccgatctcgg gttgtcgatg ggcaccggca ccgacgtggc gatcgaggcc tccgacatca
      661 ccctcgtcgc cggcgacctg cgggcggcgg tcgacgccat ccggctgtcg cgtcgaacgt
      721 tgtccacgat caagggcaac ctgttctggg cgttcgccta caacgtcgcc gccatcccgc
      781 tcgcggcgtt cgggctgctg aatccgatgc tcgccggcgc ggcgatggcg ctgtcgagcg
      841 tctttgtcgt atccaacagc ctgcggctgc gccgcttcgc ttcgatctcg acggaacgcg
      901 gatgagcccc gccgatttcg actcacctgg agtgggcgac cagcaccttc cagagccggt
      961 gtgtcacaac gcaaccgatc cgggctacat ggccgacaag gccaagtatc tcgctcggat
     1021 caggcgcatc gaagggcagg tccgaggagt tcaccggatg gtggacgagg agcagtactg
     1081 catcgacatt ctcacccaga tcgccgcgtt gcgttccgcg atgcagagtc tcgcggtgga
     1141 gctgttgggc gaccacctga gccactgtgt ggcggacgcc atgcagtccg gcggtgagga
     1201 cgcgcaggcc aagatcgccg aggccaaagc cgccgtcgcc cgcctggcga agtagtccag
     1261 cgcggccggg gctccacagc cgccgcggga gttatcgggt cgacgcgggg ttgtacgctt
     1321 ggttgagcgc cgcggccggc cgaacatcgc cgccatcgga gcgtgccgca cggagtccca
     1381 ccgattccgt gtcagcctcc gccgggcgcc ccacacgaag gacgacgaac atgcctgaac
     1441 tccgctcacg gaccgtcacc cacggacgca acatggccgg cgcccgctcg ctgctgcggg
     1501 ccgccggtgt cgacggcaac gacttcggca agcccatcat tgccgtggcg aacagcttca
     1561 ccgagttcgt gccgggacac acccacctgg ccccggtcgg gcggatcgtc agcgccgcgg
     1621 tgaaggccgc gggcggcatt ccgcgcgagt tcaacacgat cgcggtcgac gacggcatcg
     1681 cgatgggcca cggcggcatg ttgtattccc tgccgtcgcg cgacctcatc gccgactcgg
     1741 tcgagtacat ggtcgaagcg cattgcgcgg acgcactcgt gtgcatctcc aattgcgaca
     1801 agatcacccc gggaatgctg aacgcggcgt tgaggctcaa cattccgacg gtgttcgtct
     1861 ctggcggtcc gatggaaggt ggggaggcca aactggtcaa tggcaccgtc cgcaaggggt
     1921 tgaacctcat caccgtgatc gccgaggcgg tcaacgacga ggtgagcgac gaggacatca
     1981 accgtcttga ggagtcggcc tgtcccacgt gcgggtcgtg ttcgggcatg ttcaccgcca
     2041 attcgatgaa ctgtctgacc gaagcgcttg gcctgtcgct tcccggcaac ggctccacgc
     2101 tcgccaccca caccgctcgg cgcgacctct acgaggcagc cggcgctctc gtggtcgaac
     2161 tcgcgcatcg ctactacgac aatgacgacg cgtcggtgtt gccgcgcaac atcgccacct
     2221 acgacgcgtt cgagaacgcg atgagcctcg atatcgccat gggtgggtcc tccaacaccg
     2281 tgttgcacct gctcgcggcg gcgaacgagg cgggggtgcc gttcacgatg gcagatatcg
     2341 accggttgag ccggacggtc ccctgcctgt cgaagatcgc accgaacggc acctacctga
     2401 tggaagacgt ccaccgtgcc ggcggcgtcc ccgcgttgct cggcgagctc gaccgggccg
     2461 gcctgttgcg ccacgacgtg tcgtgtgtgc attcggtgag cttgaagcag tggcttgcag
     2521 actgggacat ccgcggtggc gccgcgaccg acaaggccgt cgagttgttt cacgccgcgc
     2581 cgggctgcgt gcgctccgct gaggcgttca gccagtcgga gcgctggcac agcctcgacc
     2641 tcgatggtga gaacggttgc attcgctcgg tcgatcatgc gtattccgcc gagggcggct
     2701 tggcgaccct gttcggaaac atcgccgagg acggttgcat cgtcaagacc gctggcgtcg
     2761 acgagtcgat cttcgtgttc agcggcccgg cgggggtgtt cgaatcccag gacgacgccg
     2821 tcgaggcgat cctgaaagat gagatccacg agggtgacgt cgtggtcatc cgctacgagg
     2881 ggccccgcgg cggtcccggg atgcaggaga tgctgtaccc gacgtcgtat ctgaagggaa
     2941 agggtctcgg cgccaagtgc gcgttgatta ccgacggccg tttttcgggt ggcacctcgg
     3001 gtctgtcgat cggccacatc tcgcccgagg ccgccgcagg cggagcgatc gctcttgtgc
     3061 gcaacggcga tcgcatcaat atcgatattc cgaaccgttc gatcgaactc gacgtcccgg
     3121 cggccgaact cgccgagcgc cgtggcgaac tcgaggccga caacggctac aagcccgtgg
     3181 cgcgcgaccg gaaggtgtcg gcagcgttga aggcctacgc agcgatggcc agttcggccg
     3241 accgcggcgg ggtccgcgac ctgagcatca tcggcatcgt cgactgaacg gtgctcggcc
     3301 ggcgctcggc tgccggtcag tcgacctcgt ggccggccgc aaggtcggag acgtccgaga
     3361 cgcgaagcgt ggacccgttg gtcgtgtcgc cgtcgctgcc gtcggcggcg tcggtgccgc
     3421 cggcagcgtg tgcgtgcgac ggtgcgagga cgaccccgac gtggtcgagg tcagggtgat
     3481 ccaggagatc gacgatcggg ccactcacgt gcgcctcggg gagggcgagg ccggcgtggg
     3541 ccatggtctc ccacttgtcg atgtcgtcgc cggagcgcgt ggcgaacagc gtggcaacct
     3601 cgcggtcggc gtcggtgagg gggtgaacgc tcaactccga ggcatccgcg aggaggccga
     3661 aggtgtggtt ctcgcgggag atccacacgc agtagcgcag cggttcgatg cccacctggg
     3721 agtggaaccc gaccaggcat cccgatcggt cgcctgtcgg cgtggtgacg acggcgataa
     3781 cgaccgccgc atcgagttgt cggttcacgg catcgaagcc gggcagtccg ctcatgttcg
     3841 atctccttag cgcgggcgtg cgcggtgccc gtctgcgaac gtcatcccat cacatgtcgg
     3901 cgccgggcgc gtgggaaccc ggatgaacgc ggccgaccca gggcagagcg ccccatctcc
     3961 aaagctgcac catcctgaac gttgcgcaaa ctgcaaggtt gcaccatcgg caaagttgca
     4021 taacgtgcaa taaagcgcga ccatggtgcc catgaccacc accggacttc gcgagcgaaa
     4081 aaagcgcgag accttcgtgc gcatcgtcac ggcggcgcgt gaactcgtca gcgaacgtgg
     4141 gatcgccggc gtgaccgtcg acgacatcgc ggaagcagcc ggcatctcga ctcggacctt
     4201 cttcaactac ttctccagca agaacgaggc ggtcatcggc agcgaccctc aaggcttcga
     4261 agccgccgcg tctgcattgc ggaatcggcc gagcgacgaa cgcccagcga gggcgctgtg
     4321 gagcgtcatc accggtgagg gtgacgacgc gatgctgcag cgttggatgt tgcgcaacga
     4381 acttgttcgc cgccacccgg agttgttgcc ccggcacctc gaatcgttcg acctcgccac
     4441 ccgggccctc accgaggccg tcgccgatcg gatggacgtc gaccagggac gcgatccgcg
     4501 gccggccttc gtcgtcgcca ccacgatcgc ggcgctgcgt tcggtgatgg cgtggtggca
     4561 ggcctctgat cgcagtaccg atcttcacgt ccacctcgcc acgaccctga caaccctcga
     4621 aatgctcgac cttgacgcgt tccgacccga ccccgtcgat cccgtcgacc ccgtcgaccc
     4681 cgtctctgcc cgtcgcgaaa ggatctcatg acctccaccg tgcctcgtca ggaactcacc
     4741 gcgtccggct acacgcatcg tcaggtgctc gtgatcttca gcggactgat gttgggtatg
     4801 tttctcgccg cgctcgacca gaccatcgtc gcgacggccc tacccgagat cgtgggtgac
     4861 ctcggcggcg ccagccatct cgcctgggtg gtcaccgcct acatgttgac ggtgtcgatc
     4921 tcgaccccgc tgtatggcaa gttgggcgac ctgtacggcc gtaagcggct ctttcagttc
     4981 gccatcgtca cgttcctggt gggctcgatg ctctgcggag tctcgcagtc gatgtcgcaa
     5041 ctcatcgggt tccgcgcgtt gcagggcctc ggcgccgggg ggctcatcgt gttggcccag
     5101 gcgctgatcg ccgaggtcgt caccgctcga gaacgcggtc gctatcaggg gtatttcggg
     5161 gcgcttttcg gcgtgtcgag cgtggccggt ccattgctcg gcggattcct gaccgacaac
     5221 ttgtcatggc ggtgggtctt ctacgtgaac ctgcccgtcg gattggtcgc cctggtggtg
     5281 accgccttga agctccccgc caacggcgct ccgcgttcga agccgaggat cgactacctc
     5341 ggtgccgcgc tgctggccgc ctcggtgacc tgcatcgtgt tggtcacgac atggggtggg
     5401 gaacagtacg cctgggggtc gagcctcatc ttgagcctca tcgccgtttc ggtcgtgttg
     5461 ttgggtgcct tcatcgccgt cgaacgccgc gcccctgagc cggtgttacc gctgcatctg
     5521 ttcaacctgc gcacattcgc ggtgtcgtcc gcggttgcct tcatcgtcgg cgtgggcatg
     5581 tacggcacga tcagttacct gccctatttc atgcagacgg tcaacggcgc ctccgcaacc
     5641 gattcgggcc tggtgctgtt gccgctcatg ttggcgatgc tgttggcctc gatggttgcc
     5701 ggccagagca tctcgcgcac gggtcgctat cggattttcc cggtgtcggg catggtcatc
     5761 gcggcggttg ccatgttcct gttgtcgacg atgggcggcg acacctccaa gacgctggtg
     5821 accggctaca tggcgctgtt gggtgtcggg ctcgggttct gcatgcagac gctgatcctc
     5881 gcggtgcaaa acgaggtcga gaccaaggac ctcggggtcg gaacctcggc ggtgaacttc
     5941 tttcggtcga tgggaggatc gctcggtgtg gcggtgttcg gtgcgatgtt caatgcccag
     6001 ttcgcacgca acctgccgtc gttcacccac cagctcagcg aggcggaacg cgcggcctta
     6061 cgcctcgcgg acccagcgga atacgcgcac tacgtgtacc gattcgccga ttcgatcacc
     6121 tcgatcttcg gttgggccgc ggtggtgctc gccatcgggt tcggcttgtc gctgatgttg
     6181 cgggacacga cctttggcac cggagccatc catggctcag gcgatcacga cggctccgac
     6241 ggcactcgaa cgatcggtga ccgcgtggtc gcccaatgat tccctcgtcg gcgccgtcgg
     6301 ccgtcgggtg accaatggca tactccatga catgactgga ttgacgatat gaacagcgtt
     6361 cgcactcctg acgaccgatt tgtcgggcta cccggcttcg actttgagcc gaggtatcac
     6421 gaggtgggcg acgggctgcg aatgcactac ctcgacgagg ggccgagaga cggtcgcgtc
     6481 gtggtgttgc tgcacggcga gccctcgtgg agctacctgt accgaaccat gatcccggtg
     6541 ctgacggcgg cgggttgtcg ttgcgtcgtt ccggatctca tcgggttcgg ccgatccgac
     6601 aagccgaccg agcagtccga ctacacctac gcccgccaca tcgcctggac caccgagctg
     6661 ttggtggacc atctcgacgt gctcgacgcg gtcttcttcg gccaggactg gggcagcctg
     6721 gtggggcttc gcgtcgtgac agcgcatccg gaccgtttcg ctgcgatcgc aatcggcaac
     6781 ggcgggttgc cgaacggtga catggcgatc tccgacgcgc tgcgcgcctg gcgcgagttc
     6841 gcggccaccg ccgatgagtt cccggtcggg gcgatcatca acggcgggtg cacgaccgat
     6901 ctgtccgccg aggtgatcgc cgcgtatgac gcacccttcc ccgacgactc gttcaaggct
     6961 ggcgcgcgga tctttccgtc gctcatcccc gtgtctcccg acgacgtcga ggcgcggaat
     7021 cagaaggcgg cgtggaaggt gctcgagggt tgggcgaagc cgttcctgtg cgtctattcc
     7081 gac
//
LOCUS       DAOOMA010000055         7002 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_12747_length_7002_cov_4.457176, whole genome shotgun sequence.
ACCESSION   DAOOMA010000055 DAOOMA010000000
VERSION     DAOOMA010000055.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 7002)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7002)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7002
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12747_length_7002_cov_4.457176"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..452
                     /locus_tag="PLP95_02635"
     CDS             <1..452
                     /locus_tag="PLP95_02635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB44726.1"
                     /translation="PPIPRKDLPPYIDSVIDAGVLGCGVRVRAPGWTMIDPDQGGRVS
                     GEYCIDSAEAAGVRTALVEWSCSSLEIGDAEAAEWTRWHNTILREVAASIPGTITIPP
                     SDAVCVDGDPAGSPTPAKDKAWGHEVHPVDLVWLWSVQLGPTLLAKR"
     gene            complement(476..1381)
                     /locus_tag="PLP95_02640"
     CDS             complement(476..1381)
                     /locus_tag="PLP95_02640"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HPB44727.1"
                     /translation="MPDPAENGYHFAGRSAVVTGSSRGIGRAVAHALAAQGAAVIVNG
                     RDPEVARQAAEEIAGAADVVASGGTAVAVSGSPSEPEVAEALIAAAVEATGRIDVLVN
                     CAGTAEPSGSSILNVTTAQWNELIESHLNTTFQTCRVAASQMVAAGGGAIVNTSSHAF
                     LGIYGGTGYAAGKGAVNSLTAAIAAELREHNVRANVVCPGATTRLSTGTDYEQHLRDL
                     HERGLLDDLMLAGSQNPGSPEHVASLYAFLASDLAAGITGEVFVGAGGYVGRFPKSAD
                     EFVTWRDHATNPPYTLEELAAILGG"
     gene            complement(1386..>1679)
                     /locus_tag="PLP95_02645"
     CDS             complement(1386..>1679)
                     /locus_tag="PLP95_02645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB44728.1"
                     /translation="PGAVAAKYGCEPDPVVTTLSPNPDPVTRGPITKTTWNCDAVGSA
                     AEFYIIGGGGHSWPGSKFFTQIEGIVGKTASSIDATEIIWEFFSQHRLPDAPA"
     assembly_gap    1680..1689
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1690..2217)
                     /locus_tag="PLP95_02650"
     CDS             complement(<1690..2217)
                     /locus_tag="PLP95_02650"
                     /inference="COORDINATES: protein motif:HMM:NF012958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHB depolymerase family esterase"
                     /protein_id="HPB44729.1"
                     /translation="MNERSEKIKAAGAVRTYRLTVPKNHVAGTSDPIPLVLDFHGLLE
                     GWVGTHPYSTQFSALAQKEGFAAVFPVGSQNGIHWNVNLTETNSDLRFIDALLEHLES
                     TMCIDRSRVYITGLSYGASMTSMLMCMRANTFAAAAPVAGMMNPCTRTERAVPFVTFH
                     GDADWILPFAFFKDTP"
     gene            2456..3307
                     /locus_tag="PLP95_02655"
     CDS             2456..3307
                     /locus_tag="PLP95_02655"
                     /inference="COORDINATES: protein motif:HMM:NF025008.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HPB44730.1"
                     /translation="MTTPNILNDTTPAIDESDPTLVHSVITDAWGVYFAQGGVVMAAA
                     LRAMDVVLDRPDLSLASASATFVRPVACGPVETSVDVLRSGRRGAQVHGLLRDQTAPE
                     GAVSVSVSAVFTDPAMAGPSQSPVPPRTELAIAPPPDAAMAVDNQFPLGFLDNTAWHV
                     AVGPDVSPFSTDPVPRMALWFRFNDPPAPDDVAWNPALLPIPGDALGSALSVALEDGG
                     RAVGSVSLQIDLQVLAPIVGTWIGIDTMCTHVGNGLARGVLHLWSETGVHVATVTQTA
                     MLRGFDG"
     gene            complement(3314..3865)
                     /locus_tag="PLP95_02660"
     CDS             complement(3314..3865)
                     /locus_tag="PLP95_02660"
                     /inference="COORDINATES: protein motif:HMM:NF014664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HPB44731.1"
                     /translation="MTEPARIDPGERRDVGWINAGIAGLLGRVAGTAPPHLFLTLGRH
                     RRLFRGWLIFGGALMPGGRLRRRETEMVIIRVAHRRGCAYELEHHRRLGAKAGLTPAD
                     IDAILADGTEPDSRWSPRESTMLAAADELLAERNLSDETWAQLARHLDERELIEFSLL
                     VGHYDMLATTIMTLRIQPDRPRR"
     gene            complement(3862..4740)
                     /locus_tag="PLP95_02665"
     CDS             complement(3862..4740)
                     /locus_tag="PLP95_02665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020378813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HPB44732.1"
                     /translation="MGRQRNNHAEALRGRRVLITGAARGIGATLAHRLHERGAIVAVA
                     GLEPELLAVVADSVEGPHWYLDVTNREQVDEVVDDAARRLGGLDVVVANAGIAAQLPI
                     VDGDPTIFEQTVAVNLLGSYYTLRAAGRHVSHPNGYAVAVSSLAAAVHVPLLSAYNAS
                     KAGVEALGNTLRQEMRPSGARVGVAYFAELDTDMTSRGFGTQAAKSFLGERSLTRVTP
                     LVKGIDALERGIARRSRTIYAPWFVAPILPTRRIAQGVVELVAKGRVGDVLEIAREEH
                     VDLTTPQPESEPRTGS"
     gene            4830..5270
                     /locus_tag="PLP95_02670"
     CDS             4830..5270
                     /locus_tag="PLP95_02670"
                     /inference="COORDINATES: protein motif:HMM:NF024212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HPB44733.1"
                     /translation="MDTSVTPGVEMAEQLAAAWANIERSLSRSISGVLGVSYSEYRIL
                     NALAAAPDHRASRVDLADAVGLTPSGVTRALKPLEKIGMVDSVKHERDARLTLATLTR
                     SGARTVANANSVIAERCETLLANAPTARRQVEQLTSMLAELASA"
     gene            5327..5935
                     /locus_tag="PLP95_02675"
     CDS             5327..5935
                     /locus_tag="PLP95_02675"
                     /inference="COORDINATES: protein motif:HMM:NF025736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HPB44734.1"
                     /translation="MAATIGAAALLVAVCWFGATTKAPPAWEISVFDFFNTLPSPVGN
                     ALAALMWFGTTVGILIAALAASLGKRNDISTHVLLAGLLAWLTQRSLKPLVDRGRPRD
                     LLAEANFHGHLASGGGIPSGHTAMAVAICAAVAFHVSRPWRAALALLAGAIGIARMYT
                     GNHFPLDIVAGAALGWVCAVAAHYLIGAWERRRPRSSGAATS"
     gene            complement(5948..6403)
                     /locus_tag="PLP95_02680"
     CDS             complement(5948..6403)
                     /locus_tag="PLP95_02680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB44735.1"
                     /translation="MRRKKKVKVEVGGMVHRGGLEYRINLRTHSRIVARDQRAFGPRK
                     LVVVFAEMDGNVISIAHTDRLDPPEAGIVPCIARLGCDASIAMAFCDEEVVAGPPPHD
                     LRERFRYAYDAASRWGVHLLDWVACDDLLFRSTRAVLFPDEDEWPYSAY"
     gene            6593..>7002
                     /locus_tag="PLP95_02685"
     CDS             6593..>7002
                     /locus_tag="PLP95_02685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020379419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HPB44736.1"
                     /translation="MVLDIDASLVEIHSENKQNTAGTYKHGFGFHPMFCFADATGETL
                     AALLRPGNAGANTVVDHVTVLDTAINQLPASIAVGHGAGDDPALAERQVVLRADSAGC
                     THGFLAACRQRNVGLYAHPETVSVPTTHHSPTHH"
BASE COUNT         1188 a         2397 c         2211 g         1196 t
ORIGIN      
        1 acccgccgat cccacgcaag gatctgccgc cgtacatcga ctcggtcatc gacgctgggg
       61 tgctggggtg tggggtccgc gtccgagccc cgggttggac gatgatcgac cccgatcagg
      121 gcggacgtgt cagcggcgaa tactgcatcg attccgctga ggccgccggg gtacgcacgg
      181 cgctggtcga atggtcgtgc tcgagtctgg agatcggcga tgccgaggcc gccgaatgga
      241 cccgctggca caacacgatc ctgcgcgagg tagcagcgtc gatccccgga acgatcacga
      301 ttcctccgag cgatgccgta tgcgtcgacg gcgatcccgc ggggtccccc acgccagcga
      361 aggacaaggc gtggggccat gaggtacatc ccgtcgacct tgtctggctg tggagcgtgc
      421 aactcggtcc gacgctgctc gccaaacggt agcgccgggc gaatcggctc tccgttcagc
      481 cgccgaggat cgccgccagt tcttcgaggg tgtacggcgg attcgtggcg tggtcccgcc
      541 aggtgacgaa ctcgtcggcg gacttcggga atcggccgac atatccgccg gcaccgacga
      601 acacctcgcc ggtgattcct gcggcaagat cgctggccag gaaggcgtac agcgatgcga
      661 cgtgttcggg gctccccggg ttctgcgacc ccgcgagcat caggtcgtcc aacagtcccc
      721 gctcgtggag gtcgcgcagg tgctgttcgt agtccgtgcc cgtggacagg cgagtcgtcg
      781 ccccggggca cacgacgttg gcacgcacat tgtgttcgcg aagctcggcg gcgatcgctg
      841 cggtcaggct gttcaccgct ccctttcccg cggcatatcc ggtgcctccg tagatcccca
      901 ggaacgcatg actcgatgtg ttgacgatgg cgccgccacc ggccgcgacc atctgagaag
      961 cggccactcg acaggtttgg aacgtggtgt tcaggtgcga ctcgatcaac tcgttccact
     1021 gcgctgtcgt cacattgagg atggatgaac cggatggttc ggccgttccc gcgcagttga
     1081 cgagcacatc gatccgccct gtcgcctcca ccgccgctgc gatgagcgcc tcggcgacct
     1141 cgggctccga cggcgaaccg gaaacggcaa ccgcggtgcc gccggacgcg accacgtcgg
     1201 cagcaccggc gatctcctcg gcggcctggc gagccacctc cgggtcacga ccgttcacga
     1261 tcaccgccgc gccttgagcg gccagcgcat gggccacggc ccgaccgata ccgcgactcg
     1321 agccggtcac caccgcgctt cgaccggcga agtgatatcc gttctccgca gggtcgggca
     1381 tgggttcagg caggcgcgtc gggcaggcgg tgctgggaga agaactccca aatgatctcg
     1441 gtggcgtcga tcgaggacgc ggtcttgccg acgatgcctt caatctgcgt gaagaacttg
     1501 cttcccggcc acgagtgacc gccgcctccg atgatgtaga actccgccgc cgagccgacg
     1561 gcgtcgcaat tccaggtggt cttggtgatt ggcccgcggg tcaccggatc ggggttcggc
     1621 gacaacgtcg tgaccaccgg gtcgggctcg catccgtatt ttgcggcgac ggcacccggn
     1681 nnnnnnnnnc ggggtgtcct tgaagaacgc gaacggcagg atccaatccg cgtccccatg
     1741 gaacgtgacg aacggaaccg cccgttccgt gcgggtgcac gggttcatca tccccgccac
     1801 cggagcggcc gcggcaaacg tgttcgcccg catgcacatc agcatcgagg tcatcgaggc
     1861 gccgtaggaa agcccggtga tgtacacccg tgaccgatcg atgcacatcg tcgattccaa
     1921 atgttcgagc agcgcatcga tgaaacgcag gtcgctgttg gtttccgtca ggttgacgtt
     1981 ccagtgaatg ccgttctgcg aaccgacggg gaataccgcc gcgaatcctt ccttttgggc
     2041 gagggcactg aactgggtgg agtacggatg ggttcccacc caaccctcga gcagaccgtg
     2101 gaagtcgagc accagcggta tcggatcact cgtacccgca acgtggttct tcggcacggt
     2161 gagtcgatag gtgcgcaccg ctccggcggc cttgatcttc tcggaccgtt cgttcacggg
     2221 accgcgggtg gtcaccccgc aacccggcga cggaccggtc gcccccgccg gtggcgcggt
     2281 gctcaccggt ggtgcacagg aactgagcat cagtcccgca acgacggcga ccgcaacaaa
     2341 cctgaatcgt gacagcatcg ttctccccac gacgtcgaca ttcgaacggc cgcccctcgg
     2401 tggcctcatg tccccaacag cgtgccacgt agtggacagc ggccacaatg gaaccatgac
     2461 gacgccgaac atcctcaacg acacgacgcc ggccatcgat gaaagcgacc ccacgctcgt
     2521 gcactccgtc atcaccgacg cgtggggggt gtatttcgct cagggagggg tcgtgatggc
     2581 cgccgccttg cgggcgatgg atgtcgtgct cgaccgcccg gacctgtcac ttgccagcgc
     2641 gagcgcgacg ttcgttcgtc ccgtcgcctg cggtccggtc gaaacctcgg tcgatgttct
     2701 gcgtagcggc cgacgcggcg cgcaggtgca tgggctgttg cgcgaccaga ccgcgcccga
     2761 gggggcggtg agcgtgtcgg tcagcgcagt gttcaccgac ccggcgatgg cgggtccgtc
     2821 ccagtcgccg gttccaccgc gcacagaact ggccatcgcc ccacccccgg acgcggccat
     2881 ggcggtcgac aaccagtttc ccctcgggtt cctcgacaac acggcatggc atgttgcggt
     2941 gggcccggat gtgtcgccgt tctcgaccga tccggtgccg cgcatggcct tgtggtttcg
     3001 gttcaacgat ccgcctgcgc cagacgacgt cgcgtggaat cccgcgctgt tgcccattcc
     3061 cggcgacgcg ctgggttcgg ccctgtcggt cgcacttgag gacggtggcc gagccgtcgg
     3121 cagcgtgtcg ctgcagatcg acctgcaggt cctcgccccc atcgttggca cctggatcgg
     3181 catcgacacc atgtgcaccc acgtcggaaa cggtctcgcc cgcggcgtgt tgcacctttg
     3241 gagcgaaacc ggggtacacg tggccacggt cacccagacc gccatgttgc gaggcttcga
     3301 cgggtaatgc ccgtcagcgg cgcgggcgat ccggctgaat gcgaagggtc atgatcgtcg
     3361 tcgccaacat gtcgtagtgg ccgacgagta gcgagaactc gatgagttcg cgttcgtcga
     3421 gatgtcgagc caattgcgcc cacgtttcgt cgctcaggtt gcgctccgcc aacagctcgt
     3481 cggccgccgc caacatcgtc gactcccgcg ggctccaacg cgagtcgggt tcggttccgt
     3541 cagcgaggat ggcatcgatg tcggccggcg tcagcccggc cttggccccg agacgacgat
     3601 gatgttcgag ttcgtacgcg caccctcgcc ggtgggcaac tcgaatgatg accatttcgg
     3661 tctcgcgacg tcgaagcctg ccgcccggca tcagtgcgcc gccgaagatc agccaccctc
     3721 gaaacagccg tcgatggcgg ccgagcgtca agaacaggtg cggcggtgcc gtaccggcca
     3781 cccgtccaag caggcctgcg atcccggcgt tgatccagcc gacgtcgcgg cgttcccctg
     3841 ggtcgatccg ggcgggttcg gtcacgaccc cgtccgcggt tcgctctccg gctgtggcgt
     3901 cgtgaggtcg acgtgttcct cgcgagcgat ctccaagaca tctcccacgc gacccttggc
     3961 caccagttcg accacgcctt gcgctatacg acgcgtcggc aggatgggtg cgacaaacca
     4021 cggtgcatag atggtgcgcg accgtcgggc gatcccccgc tcgagcgcgt cgatgccctt
     4081 caccagcggc gtcaccctgg tgaggctccg ctcgccgagg aacgacttcg ccgcttgcgt
     4141 tccgaacccg cgtgaggtca tgtcggtgtc gagttcggcg aaatacgcga cgccgactct
     4201 ggcacccgac gggcgcattt cttgtcgaag ggtgttgccg agtgcctcga cccccgcctt
     4261 cgaagcgttg taggcgctga gcaacggcac gtgaaccgca gcggcaagcg acgacaccgc
     4321 cacggcgtat ccgttcggat gcgacacgtg cctaccggcg gctcgcagcg tgtagtagct
     4381 gcccaacagg ttcaccgcga ccgtctgttc gaaaatcgtc gggtcaccgt cgacgatcgg
     4441 gagttgtgcc gcgatgccag cgttcgcgac gacgacgtcc aggccaccaa gtctgcgtgc
     4501 ggcgtcgtcg acgacctcgt cgacctgttc gcggttcgtc acgtcgagat accagtgcgg
     4561 cccctcgacc gagtccgcca ccacggcgag cagttcgggt tcgagcccgg cgacggcgac
     4621 gatcgctccg cgttcgtgaa ggcgatgcgc gagcgtcgca ccaatgcctc gcgccgctcc
     4681 ggtgatgagt acccgccgtc cccgtagagc ctcggcgtgg ttgttacgtt gcctgcccac
     4741 gcgctgcaac gtagcagcgg agctctactc tgcgctgtga gatcgaaatc gatatacgtg
     4801 agccctcacg gattgctaca gtgttgctca tggatacctc agtgacaccc ggagtcgaga
     4861 tggccgaaca actcgccgcg gcgtgggcca acatcgaacg ctcgctctcc cgatcgatct
     4921 caggagtact cggcgtgagc tactccgagt accgaatcct gaacgcgctc gcggctgcac
     4981 ccgatcaccg agcgagtcga gtcgatctgg ccgacgcagt cgggctcaca ccatcgggag
     5041 tgacccgagc gctcaaaccg ctagaaaaga tcgggatggt cgacagcgtg aagcacgaac
     5101 gcgacgcgag gctcacactc gcgaccctca cccgatcagg cgctcgcacc gtcgccaacg
     5161 cgaactccgt catcgctgaa cgatgcgaga cgctcctggc caacgctccg acggcccgcc
     5221 gccaggtcga acagctgacc tccatgctcg cggagttggc gagcgcctag cggggccagc
     5281 aatgggcaca tcgaacccgg tgacaccaag ccgaacggcg gctccgatgg cagcgaccat
     5341 cggagccgcc gcgctcctcg tcgctgtgtg ttggttcggg gcgacaacca aggctccccc
     5401 ggcatgggag atctcggtct tcgacttctt caacacgctt ccgtccccgg tcggaaacgc
     5461 gctggccgcg ctgatgtggt tcggaaccac cgtcggaata ctgatcgccg ctctggctgc
     5521 gtcgttgggg aagcgcaatg acatcagcac ccatgtcctg ctggccggat tgctcgcctg
     5581 gctgacacaa cgatcgctca aaccactcgt cgaccgcggt cgaccgcgag acctcctggc
     5641 cgaggccaac ttccacggac acttggcctc gggcggcggc attccatctg ggcacaccgc
     5701 gatggccgtg gccatctgcg ccgcggtggc gttccacgtg agtcggccat ggcgcgccgc
     5761 cctcgccctc ctcgccggag ccatcggcat cgctcggatg tacaccggca accatttccc
     5821 cctcgacatc gtcgctggcg ctgccctggg atgggtatgc gccgtggcag cccactacct
     5881 gatcggggca tgggagcgac gacgtccgcg cagctccggg gctgcgacgt cgtaaatcga
     5941 accccgatca gtacgccgaa tacggccact cgtcctcgtc aggaaacagc acggcccggg
     6001 tgctgcgaaa caacaggtca tcgcacgcaa cccagtcgag caggtgcaca ccccaccgcg
     6061 acgcggcgtc gtaggcgtac ctgaaccgct cacgaaggtc gtggggcggc ggtccggcga
     6121 ccacctcctc gtcgcagaac gccatcgcaa tactcgcatc acagccgagc cgagcgatgc
     6181 acgggacgat cccggcctcc ggcggatcga gccggtcagt gtgagcgatg ctgatcacgt
     6241 tcccgtccat ctcggcgaac accaccacga gcttcctcgg cccgaacgca cgttgatcgc
     6301 gggcgacaat cctgctgtgg gtacgcaggt tgattcgata ctccagccca ccccggtgca
     6361 ccatccctcc gacttcgacc ttgaccttct tcttgcgcct cacggcttgc tctctggcga
     6421 tcggcgcgcg gccccgccga cggccaggct cctcttcctc acaggtcctc cttcaacggc
     6481 gggccgggcg acccgcgttc cgagcaacct acgaacagag ccgcggcgct tctatggggg
     6541 gcgtcccccc actcaaatcc ggcgaaccgc caacatcgct gctggcgggc cggtggtgtt
     6601 ggacatcgac gcgtcactcg tggagatcca ctccgaaaac aagcagaaca ctgctgggac
     6661 ctacaaacac gggttcgggt tccacccgat gttctgtttc gctgacgcga cgggcgaaac
     6721 actcgccgcg cttctacggc cgggtaacgc gggtgcgaac accgtggtcg atcacgtcac
     6781 cgtgttggac accgcaatca accaacttcc ggcatcgatc gctgtaggtc atggcgccgg
     6841 cgatgatccc gctctcgccg aacggcaggt ggtgctgcga gccgattcgg ctggttgcac
     6901 ccacgggttc ctcgccgcat gccggcaacg caacgtcgga ttgtacgcgc atcccgaaac
     6961 cgtttcggtt ccaacgacac atcactcacc aacccaccat ca
//
LOCUS       DAOOMA010000056         6990 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_12769_length_6990_cov_4.112761, whole genome shotgun sequence.
ACCESSION   DAOOMA010000056 DAOOMA010000000
VERSION     DAOOMA010000056.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 6990)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6990)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6990
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12769_length_6990_cov_4.112761"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..486)
                     /locus_tag="PLP95_02690"
     CDS             complement(<1..486)
                     /locus_tag="PLP95_02690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB44737.1"
                     /translation="MGFPTDIGIIDLMMGIPDPGNKESWYDFLKPNLRDAESATFKFP
                     AQYMFKEVPEDPTVDDMVEYTIGQMDRFGIDKALIGVRVGGGVQWDAAVQHPDRFLAS
                     LSVDPNEGMKAIQKINTAAQATDLKAITLFPSGCNPGVAINDKRMYPVYAKACELDIP
                     VL"
     gene            complement(587..1720)
                     /locus_tag="PLP95_02695"
     CDS             complement(587..1720)
                     /locus_tag="PLP95_02695"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HPB44738.1"
                     /translation="MGLNENRVAQLLERARREVEAGLLPSVQIAIGLDGEVVVSESFG
                     AATPQTRYNIFSATKALIATVMWQLMSEGAVAPEDPVARHFPEFAGQGKGDITIEQVM
                     IHQGGFPRAPIGPPHWADRSWRIDKMASWRLNWEPGTQFEYHPTSAHWVLGEIIERVD
                     RRDYRESVRERVLVPLGLRSLALGVAPDEQGDVADMAPIGEAPTPDELEAAFGVRTYD
                     LGEVTPEALTFFNDPAVRAIGVPGGGGMSNAADLASFYQALLHNRRPDGETFMDAAWL
                     ADGTGRIRGTLPDPILGHPSNRSLGLVIAGDDGLSSLRGMGKTVSPRTFGHNGAAGQI
                     VWADPETGLSLCYLTNGIDRNFLREARRTVAIASLAGRLTEPD"
     gene            1818..2024
                     /locus_tag="PLP95_02700"
     CDS             1818..2024
                     /locus_tag="PLP95_02700"
                     /inference="COORDINATES: protein motif:HMM:NF018595.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HPB44739.1"
                     /translation="MRGTEMKIVVDYDVCESNAVCMGVVPEIFEVRDDDFLYVLDENP
                     GEEWREKVEEAARRCPKQAITVVD"
     gene            2082..3350
                     /locus_tag="PLP95_02705"
     CDS             2082..3350
                     /locus_tag="PLP95_02705"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HPB44740.1"
                     /translation="MYLERIVVVGASLAGLTAAATLRNKGWDGELIVVDSNPQMPADR
                     PPLSKQVLRGDMSGAQAQEALSSRIEGCEVDVRLGLRVAGAGFAPDDLRLKFHNDHEL
                     TCDGVVIATGSVARWPAGFLVETPESTPNEPKLVPSLSGVHVVRNLSDAEALRDDLAA
                     GPDRLIVVGAGFIGAEVAATARGLGLEVTMIEAAPLPLNRVLGGTVGEFIAELHRDEG
                     VDLRLGVGLDEVLGADGRAVGVRLSDGTIVDGSVVVLGLGAAPQLDWLAGSGVSADNG
                     VVCDETLWCGPGVVAAGDCANWPNPLFGGARMRVEQWENAIEQGEAAALRLLAADEAN
                     DWGTPTAFSSVPWFWSDQYDVKLQMAGRPDASDELVVIDGDVSERRFAVAYRRGDLCT
                     GVVAVNRPRIAVMARMRMRESLEWSHVVPS"
     gene            3367..4014
                     /locus_tag="PLP95_02710"
     CDS             3367..4014
                     /locus_tag="PLP95_02710"
                     /inference="COORDINATES: protein motif:HMM:NF019559.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysoplasmalogenase"
                     /protein_id="HPB44741.1"
                     /translation="MNTLSGIFLAVTVVVAMIDWWAVAAERQRVEWAAKPLTMVALGA
                     ALLALDLPSSTLRWWFFVGIAFSLVGDVFLMLPSQRWFPAGLGGFLVAQVCYGVGFLS
                     TRTSTAWLLAGVVVMFTVMLAIGPTIVSYASREQQVLGVAVAMYMMAISFMAVAAFST
                     GSIVAVVGALLFVASDATIGFTAFVQPVRHQRMIIMTTYHVAQVLIVLSLPGLHS"
     gene            complement(4005..4430)
                     /locus_tag="PLP95_02715"
     CDS             complement(4005..4430)
                     /locus_tag="PLP95_02715"
                     /inference="COORDINATES: protein motif:HMM:NF023266.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ERAP1-like C-terminal domain-containing protein"
                     /protein_id="HPB44742.1"
                     /translation="MATPQEELRALNALARFRDPILIERLCERCATVVRTQNAPYTLA
                     SAMANPEHGAQVWRFVERHWEELLSRFPSSSHDRLIGGVRAFADRTLVDEVTEFLAAH
                     PIRKADLPLRQHLERARVNVAARERLSAQLRSRSKIGQE"
     gene            complement(4447..6576)
                     /locus_tag="PLP95_02720"
                     /pseudo
     CDS             complement(4447..6576)
                     /locus_tag="PLP95_02720"
                     /inference="COORDINATES: protein motif:HMM:NF013592.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
     gene            6688..>6990
                     /locus_tag="PLP95_02725"
     CDS             6688..>6990
                     /locus_tag="PLP95_02725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015439658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HPB44743.1"
                     /translation="MPTCFDVEIADGIATLRLNRPEAYNTMVPEFWFELPELVLELDR
                     SGDVRALILSSTGKHFCAGMDLGVFTGGTGSLLGGDDVEEIGRKRAALFDTVLRLQD"
BASE COUNT         1165 a         2170 c         2362 g         1293 t
ORIGIN      
        1 caggaccgga atgtcgagtt cgcatgcctt cgcgtagacg gggtacatcc gcttgtcgtt
       61 gatcgccacg cccggattgc agcccgacgg aaacagggtg atggccttga ggtcggtcgc
      121 ctgcgcagcc gtattgatct tctggatcgc cttcatgccc tcgttcggat cgaccgacag
      181 gctcgccagg aaccggtcgg ggtgttgcac agcagcgtcc cactgcaccc cgccaccgac
      241 ccgaacgccg atcagggcct tgtcgatgcc gaatcggtcc atctgcccga tcgtgtactc
      301 gaccatgtca tccacggtcg ggtcttcggg cacctctttg aacatgtact gggctggaaa
      361 cttgaacgtc gccgactcag cgtcgcgcag attcggcttc aagaagtcgt accacgactc
      421 cttgttgccg ggatcgggga tccccatcat gaggtcgatg atgccgatgt ctgtcggaaa
      481 gcccataccc acaaactagt gccgtgtctg cggacgttta cacgttgcga cggccagatg
      541 actccgctcg ctgcccgacg tggcgaagcg gcgcatgagg tcggggtcag tcgggttcgg
      601 tgagccggcc ggcgagcgac gcgatggcga cggtacggcg tgcttctcgt agaaagttcc
      661 gatcgatgcc gttggtgaga tagcacaacg acaacccggt ctccgggtcg gcccagacga
      721 tctgaccggc cgcgccgttg tgcccgaagg tgcgcggcga cacggtcttg cccatgcccc
      781 gaagcgaact caacccgtcg tctccggcga tgacgagacc gagcgatcga ttcgaagggt
      841 ggccgaggat cgggtcgggc agggtgccgc gaatgcggcc cgtaccgtcg gcaagccacg
      901 ccgcgtccat gaatgtctca ccatcgggcc ggcgattgtg taagagcgcc tgatagaagg
      961 acgcgaggtc cgccgcgttc gacatcccgc cgccgccggg caccccgatc gcccgcaccg
     1021 ctggatcgtt gaagaacgtg agcgcctcgg gggtgacctc gccgagatcg taggtgcgga
     1081 ctccgaacgc cgcctcgagt tcgtctggcg tcggcgcttc gccgatcggg gccatgtcgg
     1141 caacatcgcc ctgctcgtcg ggggcgactc cgagggccaa ggaccgcaag ccgagcggaa
     1201 caagtacccg ctcacgtacc gactcgcggt agtcgcgtcg gtcgacgcgt tcgatgatct
     1261 cgccgagcac ccagtgcgcc gaagtcgggt ggtactcgaa ctgtgtgccc ggctcccagt
     1321 tcaggcgcca cgaggccatc ttgtcgatgc gccacgatcg gtccgcccag tgtggtggcc
     1381 cgatcggtgc tctcgggaag ccgccctggt ggatcatcac ctgttcgatg gtgatgtcac
     1441 ccttgccctg cccggcgaac tccgggaaat ggcgcgcgac cggatcctcg ggcgcgacgg
     1501 cgccctcgga catgagttgc cacatcaccg tcgcgatgag ggccttggtg gccgagaaga
     1561 tgttgtagcg ggtctgtggc gtcgcggcgc cgaatgactc cgatacgacg acctcgccgt
     1621 ccaacccgat cgcaatctgc accgagggca acagcccggc ctcgacctca cgtctggccc
     1681 gttccagcag ttgcgcaacc cggttctcgt tcaagcccat gggcgaacgt taccgcctct
     1741 gttccactgt gactctgggc acgttctgag catcgcctgc aacgtgttct agagtgacgc
     1801 gcatacgcgt cacgttcatg aggggaaccg agatgaagat tgttgtcgac tacgacgtct
     1861 gcgaatcgaa tgccgtgtgc atgggggtcg tcccggagat cttcgaggtc cgagacgacg
     1921 acttcctcta cgtgctcgat gagaaccccg gcgaggaatg gcgcgagaag gtcgaggagg
     1981 ccgcgcgccg ctgcccgaaa caggcgatca ccgtcgtcga ctgacggtgc cctgcggcgt
     2041 cggtgtcgac cactgatcca acccatcgga gctgaaagac gttgtatctc gaacggatcg
     2101 ttgtcgtcgg ggcgtcgctg gctgggctca ccgctgcggc gacgctgcgc aacaaggggt
     2161 gggacggtga gttgatcgtc gtcgattcca acccgcagat gcctgccgat cggccgccgc
     2221 tgtccaagca ggtgctccga ggcgacatga gcggggcaca agcccaagag gccttgtcgt
     2281 cacggatcga aggttgcgag gtcgacgtgc gcctcggtct tcgcgtcgcc ggtgcgggct
     2341 ttgcccccga tgacctgcgg ctgaagtttc acaacgacca cgagctgacc tgcgacgggg
     2401 tggtcatcgc gaccggctcg gtggcgcggt ggccggcagg gttcctcgtc gagacccccg
     2461 agtccacgcc aaacgagccg aagctggtgc cctcgctcag cggcgtccac gtcgttcgca
     2521 acctttcgga tgccgaggcg ttgcgggacg atctcgccgc tggccccgat cggctcatcg
     2581 tcgtcggcgc ggggttcatc ggcgccgagg tggcggcgac cgctcgtggc ctcggactcg
     2641 aggtgaccat gatcgaggcg gcaccgctgc cgctgaaccg ggtgctcggc ggaaccgtgg
     2701 gcgagttcat cgcggagctt catcgagacg aaggggtgga tctgcgcctc ggagtaggtc
     2761 tcgacgaggt gctcggcgcc gatggtcgag cggtcggggt gcggctgagc gacggcacca
     2821 tcgtcgacgg ctccgtggtc gtgttgggcc tcggcgccgc gccgcaactc gactggctcg
     2881 ccgggtccgg cgtgtcggcc gacaacggag tggtctgcga cgagacactg tggtgcggac
     2941 ccggcgttgt tgcggccggg gattgtgcca actggcccaa cccacttttc ggcggtgctc
     3001 gcatgcgtgt cgagcagtgg gaaaacgcca tcgaacaggg cgaagcggcc gcgctgcggc
     3061 tcctcgccgc ggacgaggcg aacgactggg ggacgcccac tgcgttctcg tcggtgccct
     3121 ggttctggtc ggaccagtat gacgtgaagc ttcagatggc cggccgaccg gacgccagcg
     3181 acgaactcgt ggtgatcgac ggcgatgtct ccgagcgccg ctttgcggtc gcgtatcgac
     3241 gtggtgatct gtgcaccggc gtcgtcgcgg tgaaccgtcc acgaatcgcg gtcatggcgc
     3301 ggatgcgtat gcgcgagtcg ctcgagtggt cacacgtcgt gcccagctga tgacgagctc
     3361 gtcgacgtga acacactctc cggcatcttc ctcgccgtca cggtcgtggt cgccatgatc
     3421 gattggtggg ccgtcgcggc cgagcgccaa cgcgtcgaat gggccgccaa gccgctgacg
     3481 atggtcgcgt tgggggcagc gctgttggcg ctggacctcc cctcgtcgac cttgaggtgg
     3541 tggttcttcg tcggtatcgc cttctcactc gtcggcgacg tgttcctgat gctcccgtcg
     3601 cagcggtggt ttccggccgg actcggaggc ttcctcgtcg cccaggtctg ctacggcgtc
     3661 gggttcctgt cgacccgaac ctcgacggcg tggctgctgg ccggggtcgt agtgatgttc
     3721 accgtgatgt tggcgatcgg acccacgatc gtgtcgtacg cctcgcgcga gcagcaggtg
     3781 ctcggcgtcg cggtcgcgat gtacatgatg gccatctcgt tcatggccgt ggccgcgttc
     3841 tcaaccggtt cgattgtcgc ggtcgtcggg gcgttgttgt tcgtggcgtc ggacgcgacg
     3901 atcggattca ccgcgtttgt ccagccggtt cgacatcagc ggatgatcat catgacgacc
     3961 tatcacgtcg cccaggtgct catcgtgttg tcgttgccag ggcttcactc ctgaccgatc
     4021 ttcgaccggc tgcgcaactg ggcgctcaga cgttcgcgag ccgcgacgtt gacgcgtgcc
     4081 cgctcaaggt gttgtcgaag tgggaggtcg gccttgcgaa tcgggtgggc agcgaggaat
     4141 tcggtgacct catcgacgag tgtccgatcg gcaaatgccc gcacaccacc gatcagacgg
     4201 tcgtgggagg aggacgggaa ccgggagagc agctcttccc agtggcgctc gacgaaacgc
     4261 cacacctgag cgccgtgttc ggggttcgcc atcgccgacg cgagtgtgta cggggcgttc
     4321 tgtgtccgaa cgacggtcgc gcagcgctcg cagagtcgtt cgatgaggat cgggtcgcgg
     4381 aaccgtgcca gcgcattcaa cgcccggagt tcctcttggg gtgtcgccat gttgtgagag
     4441 ttcgatcaac cggtcgaact cggtgggacc tgcgtggcac gcgacgatct cgattgctgc
     4501 tgcatcaagg tccggcccga cgaactcgtc gtgcgtgcgc tggcgtcggt cgaaatggcg
     4561 ccgagccgcc tccacgacat cggcacggtc ggcgatcgtg ccggccaacc gtgcgacgat
     4621 cgcctggagg tcaccgacct cggagctgcc gtgtggcccg gcctcgaagg ggctgtgcac
     4681 atcgatgacc ggagcggtca gatcgtcgac cagagtgcgc acgacatggc ggtcttccgg
     4741 atgcgcaagc aacaacaagt tgccgcagat ggaggcgatg aggcgccaga cgctggagtt
     4801 cgactccacc gcagcgacgc cacgcagggt ctcggtgagg tcatcgaagt cgactcgagc
     4861 ggcgagaaac agcgcccagg agtcgtccag gaggccgtaa cgatcgatgg gtgtgagttc
     4921 ggcgaggttt gccatcagcg agctgcgcaa ggtcggcgcg tagtcgctgc ggtagaagcc
     4981 ggtggctccg aagttgatcg acacgaagtc gatcgcgtca ccgacctcga tggtggccga
     5041 ctggtcgttc aggatcatcg tgtggtgttc gatggtaccg gcgcgactca ccgcaattcg
     5101 catcggaacc gtccagatgg tctccggcac cgggtcgccg ccggcgtagg cgaatcgacg
     5161 ctgagacaac accagcgtcg tgtcgagaag ttcgacgctg atcaacgggt gtccaccgtg
     5221 gtgaatccac cggtccagca tgtcggcgac gtcgacgccg gacgcttcgg acaacgatcg
     5281 ccagagatcg ccggtctcgg tgttgccgta ggcgtgccgc ccgagatacg agcggactcc
     5341 gtttcgaaac gcctcgggtg tcgcccactg ttcgaacatg cgcagcatcg ctgcaccctt
     5401 ttcgtaggtg agcacgtcga acatcgcctc ggcatcggcc ggggtctcga cctcgaactc
     5461 gatcgggcgg gtgttcgcca gcgcgtcggt gtcgaaggcg gcgctgcgca tcaacgaaaa
     5521 cgaggtccag atgctccaat ccgggcggta gtcgtcggca cagctgatct ccatgaaggt
     5581 ggcgaacgcc tcgttcagcc aaataccgtc ccaccactgc atcgtgacga ggtcgccgaa
     5641 ccacatgtgc gcgatctcgt gttcgatgac cgtggcggcc cgttcggttt cggaccggct
     5701 ggcgtggctt gggtcgagca gcagcagcgt ctcgcgaaac gtcacgcagc cgaggttctc
     5761 catcgctccg aacgcgaagt ccggaacggc gatcagatcg agcttctcgc ccgggtaggc
     5821 gatcgcgtag tagtcctcga accaggcaac cgcatgcgcc gcgcattcga gagcgaagga
     5881 actcaacgcg gcaactccgg gacgatgcgc cacccgcacc tgcgtcgagc cggcggtcac
     5941 gggttcgctg atctccaggt cgcccacgac ccacgcgacc aggtaggtcg acattttcat
     6001 ggttggcgcg aaccggaagg tcacccggcc gtcgtcgtgg tgagtgcgac ccacctcgtt
     6061 cgcgttggac accgcggtga gcccctcggc gcaggtcagc gagatctcga atgacgcttt
     6121 gaactcgggt tcgtcccaac acggaaacgc ccagcgcgcg tacggtgcct cgaattgtgt
     6181 aacggccatg acttcgcttc ggccgtcgac cgagaactca gaacggtagt aaccgaccag
     6241 ttggctgttg agtgccccgc ggaactccac gcgaatcgtg gccgggccgc acggcagcgt
     6301 ctcgttggcg atgagtttca ggagttcgcg gtccggatcg agttgcgccg acaccggcat
     6361 cgagcggcca gcgtcgacgg gtccgaccgt cagcgtcgtg tcgaggatct cgaggtgggc
     6421 gcagttgcag atgatgtgcg ccgtgggctc gacgatgtcg acagtgatct cgacggatcc
     6481 gtgaaaggtc gacgcctcga ggtcgctgtc gatgtggagc cggtagttcg acggcaccac
     6541 atcgtgtggc aaacggtgcc cggtggccga gatcatgtca ttgtgttccc gtcgacttgg
     6601 tccggctgcg aagcgctcgg aacatcgaat gtcaccatgg ttagcacgac caacacccgc
     6661 gcaagcgggt tccgcagagg agcaaaaatg cctacgtgtt tcgacgttga gatcgccgac
     6721 gggatcgcaa ccctgcggct caaccggccc gaggcctaca acacgatggt cccggagttc
     6781 tggtttgaat tgccggagct ggtcctggaa ctcgaccgta gcggcgatgt gcgagcgttg
     6841 attctttcgt cgaccggcaa gcatttctgt gcagggatgg acctcggggt tttcaccggt
     6901 ggaacgggct cgttgctggg cggcgatgac gtggaggaga tcggccgcaa gcgtgccgcg
     6961 ctgttcgaca cggttcttcg attgcaagac
//
LOCUS       DAOOMA010000057         6954 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_12875_length_6954_cov_4.539498, whole genome shotgun sequence.
ACCESSION   DAOOMA010000057 DAOOMA010000000
VERSION     DAOOMA010000057.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 6954)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6954)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6954
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12875_length_6954_cov_4.539498"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..90)
                     /locus_tag="PLP95_02730"
     CDS             complement(<1..90)
                     /locus_tag="PLP95_02730"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: protein motif:HMM:NF013007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HPB44744.1"
                     /translation="MGVLNVTPDSFSDGGRYVDLDAAVSHARRM"
     gene            complement(169..810)
                     /gene="folE"
                     /locus_tag="PLP95_02735"
     CDS             complement(169..810)
                     /gene="folE"
                     /locus_tag="PLP95_02735"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003062069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0042559 - pteridine-containing compound
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HPB44745.1"
                     /translation="MTERSTTSASDLNTIDATVPAGVAASPVDLGRIEAAVREILAAI
                     GEDPDRDGLRDTPARVARMYAETSSGLHEEPAQHLKVTFDAGHEEMILVRDVPFYSMC
                     EHHLVPFFGKAHMAYIPNTEGRITGLSKLARLVDGFAKRPQVQERLTTQVADAMVNVL
                     DPRGVMVVIEAEHLCMGMRGVRKPGSTTVTSAVRGIFLTDKAARAEAMSFVQR"
     gene            complement(856..1404)
                     /gene="hpt"
                     /locus_tag="PLP95_02740"
     CDS             complement(856..1404)
                     /gene="hpt"
                     /locus_tag="PLP95_02740"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015799294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HPB44746.1"
                     /translation="MSFAERDPEVGEVLLTTEQIQARIAEIGAQLTQRYAGRSPLLVC
                     VLKGAYVFMTDLARAIDLPVEIDFIAVSSYGSSTRTSGVVRLVKDLDQDIAGRDVILV
                     EDIIDSGLTLRYLRRNLESRKPASLEVCALIARESADIEALEVNYVGFTVADEDWLVG
                     YGLDVAQRYRNLDYLARYQQGE"
     gene            complement(1462..2355)
                     /gene="tilS"
                     /locus_tag="PLP95_02745"
     CDS             complement(1462..2355)
                     /gene="tilS"
                     /locus_tag="PLP95_02745"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR02432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HPB44747.1"
                     /translation="MVGDRGEDLIGRLLERCSFPVAGTAVTCGVSGGADSLSLLVLSV
                     AAGCEVTAVHVDHGLRPDSANDALVVAEAARRFGAGFRSVRVEVGDGPNLEARARAAR
                     HEVLGDALLGHTADDQAETVLWFLARGTGPEGLAGIDPTRRPLLGLRRRETAELCAVL
                     GIDALEDPANADPRFTRNRIRSELIPLFNDIARRDVVPAIAAAARHQRDLIEGLDELA
                     RNVDPTDARSVAGSARPVAVTAMRQWWRREVQTSYAPDAAALERMLAVARGDRPRADV
                     VAGWQIARTAQRLRLVPPSER"
     gene            complement(2363..3427)
                     /locus_tag="PLP95_02750"
     CDS             complement(2363..3427)
                     /locus_tag="PLP95_02750"
                     /inference="COORDINATES: protein motif:HMM:TIGR03624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent metalloprotease"
                     /protein_id="HPB44748.1"
                     /translation="MSGAIDWPTAERVARFVSSRGQGALPVGYSAIHRQFDELTDQAQ
                     GLVEAYTGWQSTSGRARARVVDREGWVRANIASFQRLLRPLTDRVEERLRPGVGTALA
                     SRAAGVEIGAMLGWMSQRVLGQYDLLVLEDENPDEQDIVYYVGPNIAALESKFEFPSD
                     EFRLWVALHEVTHRTQFTGKPWLRDYFVEQTTELMTLADPDPTRFFAAIGRIAEGVRA
                     RRNPLDDGLVTLFASTSQRAALDRVGGMMSLLEGHGDVTMDRAGGTAVPSAERFSATL
                     SARRGEVRGTAKLVQKLVGLEAKMAQYAQGEAFIAALEEQGGREYLELAFAGPDNLPS
                     LEEIREPQRWLQRVPAAVAI"
     gene            complement(3438..3593)
                     /locus_tag="PLP95_02755"
     CDS             complement(3438..3593)
                     /locus_tag="PLP95_02755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB44749.1"
                     /translation="MYALLTLLAQEGSHVWHFWLGVLLAPPIFLAVIAIFVLYAFKVS
                     RTRYPEN"
     gene            complement(3701..3946)
                     /gene="rsrA"
                     /locus_tag="PLP95_02760"
     CDS             complement(3701..3946)
                     /gene="rsrA"
                     /locus_tag="PLP95_02760"
                     /inference="COORDINATES: protein motif:HMM:TIGR03988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016989 - sigma factor antagonist activity
                     [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mycothiol system anti-sigma-R factor"
                     /protein_id="HPB44750.1"
                     /translation="MMSENCDGALSELYAYLDGELTEDDRVAVEGHLRNCSPCLEAFD
                     FEAEIRKVIANRCQDRCPDELRARIAAAIAECAKPEN"
     gene            complement(3943..4563)
                     /locus_tag="PLP95_02765"
     CDS             complement(3943..4563)
                     /locus_tag="PLP95_02765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015799299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HPB44751.1"
                     /translation="MADPSTFTDDTAPYVDALYGAARRMTRNTADAEDLMQETFLRAF
                     RAYDRFEQGTNLKAWLYRILTNTYINSYRAKQRRPQESDLADVEDLYLYRRLGGVETS
                     QIGRSAEDQLMDLLTEGEVKEAIESLPENFRMPVLLADVEGFSYKEIAEILDVPIGTV
                     MSRLHRGRKALQKSLWNFASARRLVPDGTASGAAGGDLAPTAREAR"
     gene            4618..6033
                     /locus_tag="PLP95_02770"
     CDS             4618..6033
                     /locus_tag="PLP95_02770"
                     /inference="COORDINATES: protein motif:HMM:NF013662.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-ubiquinone oxidoreductase-F iron-sulfur
                     binding region domain-containing protein"
                     /protein_id="HPB44752.1"
                     /translation="MTAGYVVAVAPSYLIPDDACTSLSDYSALGLDAAVQAARKSTPQ
                     QLRTIVEQSGLRSRDHAALSVGRRWVQICRTTDVGTRYLVATATDPEPGSFSDRHLLR
                     TNPFAVIEGIVTAALALDTSEAFIVMRRSFEREYEILTTALAEAETAGWLEQVSIKCV
                     RADDHYLLNDERSVLEVIEGRAPIPRRLSPDVDGLFCNAERGPDGVERLPDLPNATVV
                     ETIETLANVAAIVVNGSRWFRTIGTAVSPGNLLCTITGDVNTHGVVEAELGERLYDVI
                     EAGGDGYLSASSPKAVLSGVSSAVLTRSRLSAPMSWEGLAAVGADIGRAAFTVHDEPT
                     NMIEVAHRVAAYLYVESCGLCPPCKYGGGEVTAYLARLAAGVGDQRDVEALNQRVTTV
                     ADGRRCDIPLRMYKIVSSIMQAFPGDVRDAAHRQVTPVTPSTLEHIVDILGGVAIHGD
                     TQARRRADWSTSSTPVELTRW"
     gene            6159..>6954
                     /locus_tag="PLP95_02775"
     CDS             6159..>6954
                     /locus_tag="PLP95_02775"
                     /inference="COORDINATES: protein motif:HMM:NF019581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HPB44753.1"
                     /translation="MAHDLVIRGGTIVDGTGTEAYRGDVAIDGDRIVEIGTVDATSAA
                     RVIDADGAIVTPGFVDLHSHLDAQVGWDPTMSSSCWHGVTSVVMGNCGMTFAPLRPGH
                     HEVLANMMESVEDIPASAILDGLAWNWETYGDYLDRVEEMPKGMNCGGYVGDVALRVY
                     VCGERSIEPEYRATEDELAQMAALVEEALNAGALGYSISRSLSHKVPDGRFVPGTFVD
                     PVEYRTIAEPLRRLGKGVLEAAPRYNDLDGSTWRVDEEVAWMADLSR"
BASE COUNT         1223 a         2358 c         2252 g         1121 t
ORIGIN      
        1 catgcggcgc gcgtgactca ccgcggcatc gagatcgacg tagcggcccc cgtcactgaa
       61 cgaatccggc gtgacgttca agacgcccat gatcgagggg atcatcgacc gccatggtag
      121 aggagggtgt caaatctccg atgggcgaaa atctcgaccc cgaagcgttc agcgctgtac
      181 gaaactcatg gcctcggccc gggcggcctt atcggtcagg aagatgccgc gcaccgcgct
      241 ggtgaccgtc gtcgatcccg gcttgcggac cccgcgcatc cccatgcaca ggtgttcggc
      301 ctcgatgacc accatcaccc cgcgggggtc gagcacgttg accatcgcat cggcgacctg
      361 ggtggtgagt cgttcctgca cctgcggacg cttcgcgaag ccgtcgacga ggcgggcaag
      421 cttcgaaagc ccggtgattc gcccctcggt gttggggatg taggccatgt gcgccttgcc
      481 gaagaacggg acgaggtgat gttcacacat ggaatagaag ggaacatcgc gcacgaggat
      541 catctcttcg tgaccggcgt cgaaggtcac cttcaggtgt tgagccggtt cctcgtgaag
      601 cccgctactg gtctccgcgt acatccgagc gacccgggct ggagtgtcgc gcaagccatc
      661 gcggtcggga tcctccccga tggcggcgag gatctcgcga accgcagctt cgatacggcc
      721 gaggtcgacc ggcgaggccg cgacccccgc agggacggtc gcgtcgatcg tgttcaggtc
      781 cgacgcagag gtggttgagc gctcagtcat cgagggtcga tgcttgcacg gatggctcag
      841 cgggtgcgag cgcgtctact cgccctgttg gtaccgagcg aggtaatcga gattgcgata
      901 ccgctgggcg acgtccaacc cgtagccaac cagccagtcc tcatcggcga cggtgaagcc
      961 cacgtagttg acttcgagcg cctcgatatc ggccgattcc cgagcgatca gggcgcacac
     1021 ctcgagactg gcgggctttc gggactcgag gtttcggcgc aggtagcgca gcgtgagccc
     1081 cgaatcgatg atgtcttcca ccagaatcac gtcgcgaccg gcgatgtcct ggtcgagatc
     1141 cttgacgagg cgcaccacgc cggaggtccg ggtggagctt ccatacgacg acaccgcgat
     1201 gaagtcgatc tcgaccggca ggtcgatcgc tcgcgccaga tcggtcatga agacgtaggc
     1261 gcccttgagg acacagacca gcagtgggct tcgcccggcg taacgttgcg tcagttgcgc
     1321 accgatctcg gcgattcgcg cctgaatctg ctcggtggtg aggagaacct ctccgacttc
     1381 ggggtcgcgc tcggcaaagc tcatcgtcgc caacctacgg ccgggtgccc acgggccgcc
     1441 agccacacgt ccgcaccccc gtcaccgctc ggacgggggg accagccgga ggcgctgcgc
     1501 ggttcgagcg atctgccagc ccgcgacgac atcggctcgc gggcggtccc cccgagcgac
     1561 ggccaacatt cgctcgagcg cggcggcatc gggggcgtac gaggtctgca cctcacgacg
     1621 ccaccattgc cgcatggcgg tcacggccac cgggcgggca gagccggcga cggaacgggc
     1681 atcggttgga tcgacatttc gcgccagttc atcgaggccc tcgatcaggt cccgttggtg
     1741 tctcgccgcc gcggcgatcg ccggcaccac gtcgcgccgg gcgatgtcgt tgaacagcgg
     1801 gatgagttcg cttcggattc ggttgcgggt gaaccggggg tcggcgttgg cgggatcttc
     1861 gagcgcgtcg atcccgagca ccgcacacaa ttcagcggtc tcgcgccggc ggagcccgag
     1921 caacggtcgg cgggtcggat cgatccccgc aagaccctcc gggccggtgc cgcgcgcgag
     1981 gaaccacagg accgtctccg cctggtcgtc agcggtgtgg cccaacaacg catcgccgag
     2041 cacctcgtgt cgcgccgcac gggcgcgggc ctccagattg gggccgtcgc cgacctcgac
     2101 acggacgctg cgaaagcccg caccgaaccg cctcgccgcc tccgccacca cgagcgcgtc
     2161 gttcgccgag tcgggtcgaa gcccgtgatc gacgtgcacc gccgtcacct cgcaccctgc
     2221 ggccaccgag agcaccagta acgacagcga atccgctccg cccgaaactc cgcacgtcac
     2281 cgccgtgccg gccaccggaa agctgcaccg ctcgaggaga cgcccgatca agtcctcgcc
     2341 gcgatccccg accaccggac cctcagatcg cgacggcggc gggaacccgc tgcaaccagc
     2401 gttgcggttc gcggatctcc tcaaggctcg gaaggttgtc cggcccggcg aacgccaact
     2461 cgagatactc gcgaccaccc tgctcctcga gggccgcgat gaacgcctcc ccctgcgcgt
     2521 actgggccat cttcgcttcg agaccgacga gcttctgaac gagcttcgcc gtgcctctca
     2581 cctcgcctcg gcgagcgctc agcgtcgcgc tgaaccgttc cgccgacgga accgccgttc
     2641 caccggcgcg gtccatcgtc acgtctccgt gaccctcgag caggctcatc atgccgccca
     2701 cccgatcgag tgcggcccgc tgcgaggtcg acgcgaacag cgtgacgagg ccgtcgtcca
     2761 acggattacg ccgggcgcgc acgccctcgg cgatgcggcc gatggcagcg aagaaccggg
     2821 tcggatccgg atcggcgagg gtcatgagtt cggtggtctg ttcgacgaag tagtcgcgca
     2881 gccagggttt gccggtgaac tgcgtgcgat gggtcacctc atgcaacgcc acccacaacc
     2941 ggaactcgtc gctcggaaac tcgaacttcg actcgagcgc ggcgatgttc ggcccgacgt
     3001 agtacacgat gtcttgttcg tcgggattct cgtcctcgag gacgagtagg tcgtattggc
     3061 cgagcacgcg ctggctcatc cagcccagca tcgcgccgat ctcgaccccc gcggcccgcg
     3121 acgcaagcgc ggtgccaacc ccggggcgca accgttcctc gacccgatcg gtcagcgggc
     3181 gcaacaggcg ctgaaacgac gcgatgttcg ctcggaccca gccttcgcga tccaccaccc
     3241 gggctcgggc ccgaccggag gtcgactgcc aaccggtgta ggcctcgacg aggccctggg
     3301 cctgatcggt gagttcgtcg aattggcgat ggatggcgct gtatcccacc gggagcgccc
     3361 cctgaccacg tgatgagaca aaccgggcga cgcgctcggc ggtcggccag tcgatcgcgc
     3421 cgctcacgcg acggccgtca gttctcggga taacgggtgc ggctgacctt gaacgcgtac
     3481 aggacgaaga tcgcgatgac agcgaggaag atcggcgggg ccaacaagac gcccagccag
     3541 aagtgccaca cgtgtgaccc ctcttgggcg agaagagtga gcagtgcgta catcggggtt
     3601 cagcttagga gacgaacccg tggatcgcgc aaaagcgcca ccgcccgacg tgtcgtgtgt
     3661 catccgggcg cgacgtgtcg ttgcgcccgg agggcatcga tcagttctcg ggtttggcgc
     3721 attcggcgat cgccgcagcg atccgagccc gcaactcgtc cggacaccga tcctggcagc
     3781 ggttggcgat caccttgcga atctcggctt cgaaatcgaa cgcctcaaga cacggtgaac
     3841 agttgcgaag atgcccctcg accgcaacgc gatcgtcctc ggtcagttcg ccatcgaggt
     3901 aggcatagag ctcggaaagc gcaccatcgc agttctcgga catcatcggg cttctctcgc
     3961 ggtcggtgcc aggtcgccgc ccgctgcacc cgaagctgtt ccgtcgggaa caaggcggcg
     4021 agcggacgca aaattccaca acgacttctg cagcgctttt cttccccgat gcagccgact
     4081 catcaccgtt ccgatcggca cgtccaggat ttcggcgatc tctttgtaag aaaacccttc
     4141 gacatcggcc aacaacacgg gcatgcggaa gttctccggg agcgactcga tcgcctcttt
     4201 gacctcaccc tcggtgagca gatccatcaa ctgatcctcg gcgctacgcc cgatctggct
     4261 cgtttccacc ccgccgagcc gccggtagag atacaggtct tccacgtccg ccaggtccga
     4321 ttcctggggt cgacgctgct tggcccggta actgttgatg taggtgttgg tcaggatgcg
     4381 atagagccag gccttcaagt tggtgccctg ctcgaagcgg tcgtaggcac gaaacgcccg
     4441 caggaacgtc tcttgcatca gatcctcggc atcggcggtg tttcgcgtca tgcgacgggc
     4501 cgcgccgtac aacgcgtcga cgtacggtgc ggtgtcgtcg gtgaaggtgg acggatcggc
     4561 cattcgccaa cgagggtact cccgacgcag cgccgcgtcg ccacgaggcg acaaccggtg
     4621 acggcgggct acgttgtggc ggtggcgccg agctatctca tccccgacga tgcctgcacc
     4681 tcgttgagcg actacagcgc actcgggctc gacgccgcgg tacaggccgc cagaaagtcc
     4741 acacctcaac agctgcgcac catcgtcgaa cagagtgggc tgcgaagccg cgatcacgca
     4801 gccctgtcgg tcgggcgtcg gtgggtgcag atctgccgaa ccaccgacgt gggcacgcgg
     4861 tatctcgtcg ccaccgccac cgaccccgaa ccggggagct tcagcgaccg tcacctgttg
     4921 cgcaccaacc cgttcgcggt catcgaggga atcgtcaccg cggcgttggc gctcgacacc
     4981 agcgaggcct tcatcgtgat gcgccgcagc ttcgaacgcg aatacgagat cctgaccacg
     5041 gcactcgccg aggccgaaac cgccggctgg ctggagcagg tgagcatcaa gtgcgtccgc
     5101 gccgatgacc actacctgct caacgacgaa cggtcggtgc tcgaggtcat cgaaggccgt
     5161 gcaccgattc cgcgacggct ctcccccgat gtcgacgggt tgttctgcaa cgccgagcgg
     5221 ggccccgacg gggtcgaacg cctccccgac ctgcccaatg ccacggtggt cgaaaccatc
     5281 gaaaccctcg cgaacgttgc ggccatcgtc gtcaacggct cacgctggtt tcgaacgatc
     5341 ggcactgcag tgtcccccgg caacctgctg tgcaccatca ccggcgacgt caacacccac
     5401 ggcgtcgtcg aagccgaact cggcgagcgg ctctacgacg tcatcgaggc cggtggagac
     5461 gggtacttgt cggcctcctc tccgaaggcg gtgctcagcg gggtgagttc tgcggtgctc
     5521 acccgatcgc gcctgtcggc accgatgtcg tgggaggggc tcgctgcggt cggggccgac
     5581 atcggacggg ccgcgttcac cgtccatgac gagccgacca acatgatcga ggtcgcgcat
     5641 cgagtcgccg catacctcta tgtcgaaagc tgcgggctgt gccccccgtg caagtacgga
     5701 gggggcgagg tgaccgcgta tctcgcccga ctcgcggccg gggtgggcga ccagcgcgac
     5761 gtcgaggcac tcaaccagcg ggtcaccacg gtcgccgacg gccgtcgatg cgacattcca
     5821 ctgcggatgt acaagatcgt cagttcgatc atgcaggcgt ttcccggcga cgtccgcgac
     5881 gccgcgcatc gacaagtcac cccggtcacc ccttccacac tcgaacacat cgtcgacatc
     5941 ctgggtgggg ttgcgattca cggcgacact caggcgcgcc gccgagccga ctggtcgacc
     6001 tcctccaccc ccgtcgagct gacccgctgg tagcaaatca ccgcggaagc ggccgtctca
     6061 gcggtgccga cgcagtgccg acgcagtgcc gacgcagtgc cgaacgatcg ccgcacctac
     6121 catgaacccg cgcacgcaaa cgttcagggg gactcgacat ggcacacgat ctggtgattc
     6181 gaggcggcac gatcgtcgac gggaccggca ccgaggccta ccgcggtgat gtggcgatcg
     6241 acggcgaccg tatcgtcgag atcggcacgg tagatgccac gtcggcggcg cgggtgatcg
     6301 acgccgacgg agccatcgtc acccccggct tcgtcgacct gcattcgcac ctcgacgctc
     6361 aggtcggatg ggatccgacg atgagttcct cgtgctggca cggggtcacc tcggtggtga
     6421 tgggtaactg cggcatgacc ttcgccccgc tgcgtcccgg ccaccacgag gtgctcgcca
     6481 acatgatgga atcggttgag gacattcccg cctcggccat cctcgacgga ctcgcctgga
     6541 attgggaaac ctacggcgac tacctcgacc gggtcgagga gatgcccaag gggatgaact
     6601 gcggcgggta cgtcggcgat gtcgcgctac gtgtgtacgt gtgcggcgaa cggtcgatcg
     6661 aacccgagta ccgcgccacc gaggacgaac tcgcacagat ggccgcactc gtggaggagg
     6721 cgctcaacgc cggcgccctc ggctattcga tctcgcggag cctgagccac aaggttcccg
     6781 acggacgatt cgttccgggg accttcgtcg acccggtcga gtaccgcacg atcgccgaac
     6841 cgctgcgacg gctcggcaag ggcgtgctcg aggcggcacc gcggtacaac gatctggacg
     6901 ggtcgacctg gcgggtcgat gaggaggtcg cctggatggc cgacctcagc cggt
//
LOCUS       DAOOMA010000058         6935 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_12934_length_6935_cov_4.062209, whole genome shotgun sequence.
ACCESSION   DAOOMA010000058 DAOOMA010000000
VERSION     DAOOMA010000058.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 6935)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6935)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6935
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12934_length_6935_cov_4.062209"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..275
                     /locus_tag="PLP95_02780"
     CDS             <1..275
                     /locus_tag="PLP95_02780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB44754.1"
                     /translation="WRRELSAILADIDAGDYESVSPQRLPVELGPIPEELTELANLVV
                     ERIGHVTTLCRSRLCEIDSELRGTTSRGPRRWAHSSPEPSRLDTSA"
     gene            545..886
                     /locus_tag="PLP95_02785"
     CDS             545..886
                     /locus_tag="PLP95_02785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB44755.1"
                     /translation="MISDLTLETLGSGLRGLNLRKSQHEDAIANVETPGYRARRVDFE
                     SQLTRAVDAGTPQTARATTTFTNDAPLPNGNNVQLEREITSLTETALSQQLLISAANA
                     KYGLLRTVITG"
     gene            892..1314
                     /gene="flgC"
                     /locus_tag="PLP95_02790"
     CDS             892..1314
                     /gene="flgC"
                     /locus_tag="PLP95_02790"
                     /inference="COORDINATES: protein motif:HMM:TIGR01395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009425 - bacterial-type flagellum basal
                     body [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgC"
                     /protein_id="HPB44756.1"
                     /translation="MGAMFSALGVAASGMSTYKVWLDAIADNVANVSTVRGMDENAYE
                     SKYVVAQSRPGSGHGVRGQIGRGTSVSRIVENDPEGILAYQPDHPLANEEGLVRLPNV
                     DLGEQMTNMIIAQRAYQVNTSVFERARDSYLRALEIGK"
     gene            1317..1637
                     /gene="fliE"
                     /locus_tag="PLP95_02795"
     CDS             1317..1637
                     /gene="fliE"
                     /locus_tag="PLP95_02795"
                     /inference="COORDINATES: protein motif:HMM:NF014143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HPB44757.1"
                     /translation="MAIISPVSAGLSSATVAPVATPTDSNLSTRSSGAEGFDTAISGA
                     LNSLNDQHLYADQLARDAATGNLQSIEDYMIAATEAQLSTQLTVAVRNKALEAFNEIM
                     RMQV"
     gene            1643..3331
                     /gene="fliF"
                     /locus_tag="PLP95_02800"
     CDS             1643..3331
                     /gene="fliF"
                     /locus_tag="PLP95_02800"
                     /inference="COORDINATES: protein motif:HMM:TIGR00206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009431 - bacterial-type flagellum basal
                     body, MS ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body MS-ring/collar protein
                     FliF"
                     /protein_id="HPB44758.1"
                     /translation="MVDTMNPNAESSAGLAGWKAKATDSLAAFSPGQRAMMGAAAALV
                     VIGVLAMSVFGNKTEFAPLMTNLVAEDAQAITAQLDSLGVPYELAGGGSTIMVPKDRV
                     YQTRIDVASVDLPGSGKVGYGILDNTDLTSSEFSQHVNYQRAMEGELATTIESIDGVV
                     AATVHLALPKNSTFALDHQAATGSVMVKTEGNSSLSGSQVQAVVNLVSGAIENLDPSD
                     VTVTDAEGRVLASPNGPVGTGGGGVDNQETTAAYETSLAGSIESMLAKIVGEGHASVT
                     VAADLDFDSSKSVSESYAPPSTIVPGQALAINESTRSENYSGSGAGDNAAGVLGTEGS
                     AEGTNSPTDGSGGYQLDERQVNNAVDKVVEETSKAPGAINRLSVAVVLDEDAIGPDQI
                     TEVQTLVSAAAGLDPDRGDTVAISRLKLDQTATESMREELAFRENGDEPTATPIWMYA
                     VAGVAAVAVIGFVLRRRKRKNLTLEPIEMMTRELPTSERDLVEGYGGSDREDGPGELV
                     AVNAALDAASSEATSRSIDPTGRREALGDLIDNQPDEVADLLRSWLGDRRETPR"
     gene            3328..4356
                     /gene="fliG"
                     /locus_tag="PLP95_02805"
     CDS             3328..4356
                     /gene="fliG"
                     /locus_tag="PLP95_02805"
                     /inference="COORDINATES: protein motif:HMM:TIGR00207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009433 - bacterial-type flagellum basal
                     body, C ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HPB44759.1"
                     /translation="MTATMPSVDGFDGIDGLSGVQKVAVFIMSLDRQRAARVLRELST
                     EDVAAVARELERVGYVTAPTIDGVIDDFARTAERQRGDVRVTHQDVRELIELSLGPIA
                     AAELFGRVEHGRFGFLAPFEIETLVSMLADEHPQTVAVVLAHLRPERAARVLAGFDEE
                     AQRDLGIRVATMERTNPAAIDAIEHRLHDRAQTLLAELAEERVGGAEGLIALLTRSDK
                     ATERVVLAELEQIDPELADEVRAKLFTFEDIVTLDDRAVQQILRQVDTRGLAVALKGA
                     DETTKTKVLKNMSSRAAENLMEEIDLLRGVRAAEVALARTEVVKVIRTLEDSGDIVIN
                     RGLDDVVD"
     gene            4340..4975
                     /locus_tag="PLP95_02810"
     CDS             4340..4975
                     /locus_tag="PLP95_02810"
                     /inference="COORDINATES: protein motif:HMM:NF014196.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliH/SctL family protein"
                     /protein_id="HPB44760.1"
                     /translation="MMLLTDPFPTIEDLSLPGGTIAMSTQSVVDREALDTAMEAEFER
                     RRLEGYRRGLDEGRVAAMTAATPQISYALTALTEAVEDLHHRDVAGVEAFGAHVVDLA
                     MAIAEAVIGRALDTAADPGRDALVRTLALAPDRGDVAAHLNPDDLATIDAADPVFDAR
                     PIALIADSAVERGGCVLSVGSTTIDGQLGPALDRVRRELARGQGGRDEVRP"
     gene            4972..6405
                     /locus_tag="PLP95_02815"
     CDS             4972..6405
                     /locus_tag="PLP95_02815"
                     /inference="COORDINATES: protein motif:HMM:TIGR01026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliI/YscN family ATPase"
                     /protein_id="HPB44761.1"
                     /translation="MSSVLTDPAIRKALNAARPTVTGRVVRVVGLTFEIEGLPAAIGD
                     MVRLGDHPGVLGEIVALGEHGPIGMPLGELRGMQIGTPARIDAAAPGVPVGFELLGRV
                     LDGMGVPLDDGPSLDALAHVDVDAEPPHPLRRALVRQPQHLGVRALDTLVPCGRGQRI
                     GIMAGSGVGKSSLLSMIIRGTEADVSVLALIGERGREVREFIEHDLGPEGLQRAVVVV
                     ATSDQPALVRKRAAFVATRIAEWFRDQGKNVNLMMDSLTRFAMAQREIGLAAGEVPAT
                     RGYPPSMFGLLPKLLERAGAAEHGSITGLYTVLVEGDDMNDPVGDTARSILDGHVTLS
                     RSLASQGHFPTIEVLDSVSRVAGAVTDDHQQSLMRELRALLAAYREARDLIEIGAYKP
                     GANPTVDRAVALHDQINAFLRQSMNETDAPGVAWQRLRDLLATPNVADHTGGGTVPVG
                     MQLPVPGQIPMAQAMAAQTATSAGMLP"
     gene            6402..6833
                     /locus_tag="PLP95_02820"
     CDS             6402..6833
                     /locus_tag="PLP95_02820"
                     /inference="COORDINATES: protein motif:HMM:NF014144.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar FliJ family protein"
                     /protein_id="HPB44762.1"
                     /translation="MKKYRFSLAGAQRVQRIAEDQAKAALADARHDADEASAALQARL
                     AEIGSALPDTGQRSSQQFLAEREQLDRHRDAVIAARSAEANALSIMQQRHDDYVAAAR
                     KVRTLDRLDERKREEWRFEVTRAAQLVTDETATIRYRGPRQ"
BASE COUNT         1250 a         2438 c         2190 g         1057 t
ORIGIN      
        1 cgtggcggcg cgagctctcg gcgatcctcg ccgacatcga tgccggagac tacgagtcgg
       61 tctcgccgca gcgtcttccg gtggagctcg gccccatccc cgaggagctc accgaactcg
      121 cgaacctggt cgtcgaacgc atcgggcacg tcaccacctt gtgccgctca cgactctgcg
      181 agatcgactc cgaactgcgc gggaccacgt ctcgcggacc gcggcgctgg gcccactcca
      241 gcccggagcc cagccgactc gacacctcgg cctagaagta cgctggccaa cctcatctcg
      301 ccgaccgctg ggccgcgaga cccaccaccg atggtcgatc atgggtcgac cgtcgggttt
      361 ttctacagat ccgcgctgat ctcctcagat tgcgcttcgg ggtgccgata gaagtcggag
      421 caacggagcg ctcacgtcac tcgccaggga ccggcgacgg accacttgtt ggagatctga
      481 tgattccgcg ttccctgcgc accgactgcg ctgcatcaac gcgatcccga tcgaggatcg
      541 tgtgatgatc agcgacctca ccctcgaaac cctcggatcg gggctgcgtg gcctgaacct
      601 gcgcaagtcc caacacgagg acgccatcgc gaacgtcgag actcccggct accgcgcccg
      661 acgcgtcgat ttcgagtcgc agctcacccg ggcggtcgac gccggcactc cgcagacggc
      721 gcgggccacg accaccttca ccaacgacgc tccgctgccc aacggcaaca acgtgcaact
      781 cgaacgcgag atcacctcgc tcaccgagac ggcgttgagc cagcagcttc tgatctccgc
      841 tgcgaacgcc aagtacggcc tgttgcgcac cgtgattacg gggtgatggc gatgggcgca
      901 atgttttccg cactcggggt cgcggcctcg gggatgagca cctacaaggt gtggctcgac
      961 gccatcgccg acaacgtcgc caacgtgtcg accgtgcgtg gaatggacga gaacgcgtac
     1021 gagtcgaagt acgtcgtcgc acaatcgcga cctggctccg gccacggtgt gcgcggccag
     1081 atcggtcggg ggacctcggt gagtcgcatc gtcgagaacg acccggaggg gatcctcgcg
     1141 tatcagcccg atcatccact cgccaacgaa gagggtctcg tgcgtttgcc gaacgtcgac
     1201 ctcggcgaac agatgaccaa catgatcatc gcccaacgcg cgtaccaggt gaacacctcg
     1261 gtgttcgagc gggctcgcga ttcgtacctt cgagcactcg agatcggtaa gtagccgtgg
     1321 cgatcatcag ccccgtctcc gccggactgt ccagtgccac ggtcgccccg gtcgcgaccc
     1381 cgaccgactc gaacctgtcg acccgctcca gcggggccga ggggttcgac accgcgatct
     1441 ccggcgcgct gaacagcctc aacgatcagc acctctacgc cgaccagctc gcccgcgacg
     1501 ctgcgaccgg caatctgcag agcatcgagg actacatgat agctgcgacc gaagcgcagc
     1561 tcagcaccca gttgaccgtc gccgtgcgca acaaggcgct cgaggcgttc aacgagatca
     1621 tgagaatgca ggtctgaggt agatggtcga cacgatgaat cccaacgccg aaagctccgc
     1681 agggcttgcc ggttggaagg ccaaggccac cgactcgctg gccgcgttct ccccgggaca
     1741 gcgcgccatg atgggcgccg cggcggcgct ggtggtgatc ggcgtcctgg cgatgagcgt
     1801 cttcggcaac aagaccgagt tcgcgccgct catgaccaac ctggtcgccg aggacgccca
     1861 ggccatcacc gcacaactcg actcgctcgg cgttccgtac gaactcgccg gcggaggctc
     1921 gaccatcatg gtgccgaagg atcgtgtgta tcagacgcgg atcgacgtcg cctcggtcga
     1981 cctcccgggt tccggcaagg tcggatacgg gatcctcgac aacaccgacc tgaccagctc
     2041 cgagttcagt caacacgtca actaccaacg ggcgatggaa ggcgaactcg ccaccacgat
     2101 cgagtcgatc gacggcgtcg tcgccgcaac cgtccatctc gcgctcccga agaacagcac
     2161 cttcgcactc gaccaccagg ccgcgaccgg ttcggtcatg gtcaagaccg aaggtaattc
     2221 ctcgttgtct ggcagccagg tccaagcggt cgtgaacctc gtttcgggcg cgatcgagaa
     2281 cctcgatccg agcgacgtca ccgtcaccga cgccgagggg cgcgtgctcg cctcccccaa
     2341 cgggccggtg ggcaccgggg gtggcggggt cgacaaccag gaaacgaccg cggcgtacga
     2401 aacctcgctt gccggatcga tcgagtcgat gctggccaag atcgtcggcg agggccatgc
     2461 cagcgtcacc gtcgccgccg acctggactt cgactcatcg aagtcggtgt cggagtccta
     2521 cgcaccaccg tcgacgatcg tgcctggcca ggcgctggcg atcaacgaat cgacgaggag
     2581 cgagaactac tcgggctccg gggctggcga caatgccgcc ggagtcctcg ggaccgaggg
     2641 ttcggccgaa gggaccaact ccccgaccga cggctccgga gggtaccaac tcgacgaacg
     2701 gcaggtgaac aacgccgtcg acaaggtcgt cgaggagacc tccaaggccc ccggggcgat
     2761 caatcgcctg tcggtcgccg tcgtcctcga cgaggatgcg atcggccccg accagatcac
     2821 cgaagttcag acgctggttt ccgccgcggc cggcttggat ccggaccgtg gcgacaccgt
     2881 tgcgatctcg cggctcaagc tcgaccagac cgcaacggaa tcgatgcgcg aggaactggc
     2941 atttcgcgag aacggcgacg aaccgaccgc caccccgatc tggatgtacg ccgtcgccgg
     3001 ggttgctgct gtggccgtaa tcggcttcgt gctgcgccgc cgcaagcgca agaacctgac
     3061 gctcgaaccg atcgagatga tgacccgcga gctccccacc agcgagcggg acctcgtcga
     3121 gggatatggg ggcagcgacc gcgaggacgg ccccggcgag ttggtcgccg taaacgccgc
     3181 gctcgacgcc gcctccagcg aggcgacctc gcggtcgatc gatccgaccg gccgacgcga
     3241 ggcgctcggt gacctcatcg acaaccaacc cgacgaagtc gccgacctgt tgcggtcgtg
     3301 gcttggcgat cgacgcgaga ccccgcgatg accgcaacga tgccgtccgt cgacggcttt
     3361 gatggaatcg acggactcag cggcgtgcag aaggtcgccg tgttcatcat gagcctcgac
     3421 cggcaacgag ctgcccgcgt gttgcgcgag ctgtcgaccg aagacgtcgc ggcggtcgcc
     3481 cgagaacttg agcgggtcgg ctatgtgacc gctccgacga tcgacggggt gatcgatgac
     3541 ttcgctcgca ccgcggaacg ccaacgcggc gacgtcaggg tcacccacca ggatgtccgc
     3601 gagctcatcg agttgtcgct cggtccgatc gccgctgcgg aactgttcgg acgggtcgaa
     3661 cacggccggt tcgggtttct tgcaccgttc gagatcgaga ccctcgtgtc gatgctcgcc
     3721 gacgaacatc ctcagacggt cgccgtcgtg ctggcccacc tgcgccctga acgcgccgcc
     3781 cgggtcctcg ccgggttcga tgaggaagcc cagcgcgacc tcggcatccg cgtcgcgacg
     3841 atggaacgaa ccaatcctgc cgccatcgac gccatcgaac accggctcca cgaccgcgct
     3901 cagacgctgc ttgccgagct cgcagaagaa cgggtcggcg gcgccgaggg cttgatcgcg
     3961 ttgttgaccc gctccgacaa ggccaccgag cgggtcgtgc tcgcggagct cgaacagatc
     4021 gatcccgaat tggccgacga ggtccgggcc aagctgttca cgttcgaaga catcgtcacc
     4081 ctcgacgatc gggcggtcca gcagatcctg cgccaggtcg acacccgcgg tctcgccgtc
     4141 gcactcaagg gagccgatga gaccaccaag accaaggtct tgaagaacat gagctctcgt
     4201 gctgccgaga acctgatgga ggagatcgac ctgctccgag gcgttcgcgc cgccgaggta
     4261 gcgctcgccc gcaccgaggt cgtcaaggtc atccgcaccc ttgaagacag cggcgacatc
     4321 gtgatcaacc gaggactcga tgatgttgtt gactgatccg ttcccgacca tcgaggacct
     4381 gtcgttgccc ggcgggacca tcgccatgag tacgcaaagc gtcgtcgacc gtgaggctct
     4441 cgacacggcc atggaggcgg aattcgaacg ccggcgcctc gaggggtacc gccgcggact
     4501 cgacgagggt cgtgtcgcgg ccatgactgc ggccaccccc cagatctcct acgcgctcac
     4561 ggcgctcacc gaagcggtcg aggacctgca tcaccgcgac gttgccggcg ttgaagcgtt
     4621 cggagcccac gtggtcgacc tggccatggc catcgccgag gcggtgatcg gtcgcgcgtt
     4681 ggacaccgcc gcggatcccg ggcgcgatgc gctcgttcgc accttggccc tcgcacccga
     4741 ccgtggcgac gtcgccgccc acctcaaccc cgacgacctg gccaccatcg acgccgccga
     4801 tccggtcttc gacgctcgcc cgatcgcact catcgcagac agcgccgtcg aacgcggtgg
     4861 atgtgtgttg agtgtcggct ccaccacgat cgacgggcag ctcggcccag cgctcgatcg
     4921 cgtccggcgc gagttggccc gtggccaggg cggccgcgac gaggtccggc catgagttca
     4981 gttctgaccg accccgcaat ccgcaaggcg ttgaacgccg ccaggccgac cgtcaccggc
     5041 cgggtcgtcc gcgtggtcgg gctcaccttc gagatcgaag ggcttcccgc cgcgatcggc
     5101 gacatggtcc ggctcggcga ccacccggga gtgctcggag agatcgtcgc gctcggcgaa
     5161 cacggcccga tcggcatgcc cctcggcgag cttcgcggaa tgcagatcgg caccccggca
     5221 cgcatcgacg ccgccgcgcc cggagtgccg gtcgggttcg aattgctcgg ccgggtcctc
     5281 gacggaatgg gcgtgcccct cgacgacggc ccctcgctcg atgccctcgc ccacgtcgac
     5341 gtggacgccg agccgccgca tccgttgcga cgtgccctcg tgcgccaacc gcagcacctc
     5401 ggagttcgtg cactcgacac cctcgtgccg tgcggccgtg gccagcgaat cggaatcatg
     5461 gccggctccg gtgtcggcaa gtcatcgctg ctgtcgatga tcatccgagg caccgaagcc
     5521 gacgtctccg tgttggcgct gatcggcgaa cgcggccgcg aggtgcgcga attcatcgaa
     5581 cacgatctcg gcccggaggg actgcagcgc gccgtcgtcg tcgtcgccac ctcggatcaa
     5641 cccgccttgg tccgcaagcg tgcggcattc gtcgccaccc gaatcgcgga gtggttccgc
     5701 gaccagggca agaacgtcaa cctgatgatg gacagcctca cccgattcgc catggcccag
     5761 cgtgaaatcg gacttgccgc aggcgaggtt ccggccacgc gcggctatcc accttcgatg
     5821 ttcggcctgc tcccgaagct gctcgaacgc gccggcgcgg ccgagcacgg cagcatcacc
     5881 ggcctgtaca ccgtgctcgt cgagggtgac gacatgaacg atccagtcgg agatactgcg
     5941 cgctcgatcc tcgatgggca cgtcacgttg tcgcggtcac tggcatcgca gggccacttc
     6001 cccaccatcg aggtgctcga ctcggtctct cgcgtcgccg gcgccgtcac cgacgaccac
     6061 caacaatccc tgatgcgcga gttgagagcc ttgctcgccg cctaccggga ggcgcgcgac
     6121 ctcatcgaga tcggggccta caagccggga gccaacccga ccgtcgatcg cgccgtggcg
     6181 ctgcacgacc agatcaacgc atttctgcgt caatcgatga acgagaccga cgcgcccggc
     6241 gtcgcatggc aacgtctccg cgacctgttg gcgacgccga acgtcgccga ccacaccggt
     6301 gggggcacgg tcccggtcgg gatgcagctt ccggttcccg ggcagattcc gatggcccag
     6361 gccatggcag cccagacggc gacgagcgcc gggatgttgc catgaagaag taccgcttct
     6421 cccttgccgg tgcacaacgg gttcagcgaa tcgccgagga ccaggccaag gcggcactcg
     6481 ccgatgctcg ccacgacgcc gacgaggcga gcgctgcatt gcaggcgcgc ctcgccgaaa
     6541 tcggctcggc ccttcccgat accggccaac ggtcatctca acagttcctc gccgagcgcg
     6601 agcaactcga ccgccatcgc gacgcagtca tcgccgctcg ctcggcggaa gccaacgcgc
     6661 tttcgatcat gcagcaacga cacgacgatt acgtcgccgc cgcacgcaag gtccggacgc
     6721 tggatcgcct cgacgaacgc aagcgcgaag agtggcgctt cgaggtcacc agagcagcgc
     6781 aactcgtgac cgatgagacg gccacaatcc gttaccgggg gccgcgacaa tgagcacccc
     6841 gatctcgatc gtcccccagc ggatcgccga aatcgaacag cgcttcgctc cagcggccac
     6901 tcgcccgacc tcgcaccgag acgccggcgc cttcg
//
LOCUS       DAOOMA010000059         6789 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_13320_length_6789_cov_3.807544, whole genome shotgun sequence.
ACCESSION   DAOOMA010000059 DAOOMA010000000
VERSION     DAOOMA010000059.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 6789)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6789)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6789
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13320_length_6789_cov_3.807544"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..578
                     /locus_tag="PLP95_02825"
     CDS             <1..578
                     /locus_tag="PLP95_02825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB44763.1"
                     /translation="SGNEADGDRSADVSGATVPDENRPALEPSDEADTGADTGADDAE
                     SSTVTAAPSDPAIDAVDDSAVGERGALEEVVSSATDPVTGNSVSDLEAVTSGLPETWD
                     RNSRGAEIRLDEAAQLACANAQIGLVRLGELSADAATGHLAVAAVRADQSAVAEIHTS
                     ANGLALAALNHSEAGAAEAFVQICVDRGYEG"
     gene            580..1362
                     /locus_tag="PLP95_02830"
     CDS             580..1362
                     /locus_tag="PLP95_02830"
                     /inference="COORDINATES: protein motif:HMM:NF013250.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HPB44764.1"
                     /translation="MSAESPVDVAAVPPEPSALARYRADIAALAAHQKSSRGAPPYSL
                     LINRPLGRRFAALAHLVGLTPNQVSLISFGFTAAGLLVFAVMSPSWPQSILATALLVL
                     GYAIDSADGQLARLTTGGSLAGEWLDHTLDSVKLGVFHGAILVSAYRFDADLGVWPQV
                     TALAFGMVAATLFLIFILTDQLRRGAGGTAPERPQAWWFMVLNAPTDYGVQCLWMVMR
                     PSSTVFFGGYGLLALANLGYLGVSGRSRFRQMQQIDRERVRR"
     gene            1359..1778
                     /locus_tag="PLP95_02835"
     CDS             1359..1778
                     /locus_tag="PLP95_02835"
                     /inference="COORDINATES: protein motif:HMM:NF013621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyltransferase/cytidyltransferase family
                     protein"
                     /protein_id="HPB44765.1"
                     /translation="MTVVGYAPGAYDLLHVGHLRLLERSRERCDRLVVGVVGDDLAEL
                     QRGERPAEPLEARMAAVAELDWVDEVVEDPSSDKAVIWERVRFDVIFKGDDWRGTPKG
                     DALEAAMARVGAEVVYLSYTKDISSTRLRRELREACS"
     gene            complement(1785..3122)
                     /locus_tag="PLP95_02840"
     CDS             complement(1785..3122)
                     /locus_tag="PLP95_02840"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HPB44766.1"
                     /translation="MSHPWRLPWRLPSLWTDTWPAVLGLAMILGTEYKFRQRELNDSL
                     SASVDLAIVVELAVFALVAGYLIGYVVTPPRWNRPTAVQFFMRGYTAAMVMSVMYSSY
                     VVLGAVRAMQLCIMVAFSSAIAANARRGQVLQLAHAYVGLVTLSVFIGLVYRVPFSPL
                     QAHRFNWLYVHSVTAGAMLALGITIALGLATNHSRFRLGAQRWPRPLYLTCLAVMGAA
                     LVATQTRGAVIAAIAGVFVVIYLTVPVRERLPLGVLGFVGLALAVFSFWPVILAYLAR
                     GEPTENLTTVSNRTELWGIAVDLVSQKPLTGWGLSTSRGVFYDRVGLGGAHNAFINVA
                     VDGGLIGLGLWLGLVIAIGLGIARLQRLRHGDAPLLGGVLACLMVNGFTVEGVGSGVG
                     VSALWLLILGAWVGVLQREVGPTQRNTQGTMALTPLAARERATRERAGNTVSV"
     gene            complement(3122..4570)
                     /locus_tag="PLP95_02845"
     CDS             complement(3122..4570)
                     /locus_tag="PLP95_02845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB44767.1"
                     /translation="MFATEPTQPQAAPGIIEAALRYRKLMTAVPLACVLIAVVFTALR
                     PNSFEAVGKVTLTDPRGSATFADGISVVDFARYSAERAAFATSSDVLQAAAQNFPRAG
                     SAADLRSNCSATAASASTVMTVTCQFGEFRTATGAVDAIVAAYRDATARQFDDKAERA
                     LAALSGESPRADSGIPGIDLSDDGQIQQRMAEINTTRALFGDGVDAYDPARVPSSSNF
                     LARLVRNVVIAGIIGFLAAVLIAWFRADREPIGENSADVSSWLGLPMLGEVGYDLVSA
                     KTIDMVAVPEPSFQRITSNIDAVLSGDTVLFTPADLMAHDADVVIKTALVAARAGKRV
                     LLIDADHGARGVSTILGLDNRLGFADVVAGSAEADEATVRLRFGENSAMTSSALFLMG
                     PGGDPSQPPALLRTGATESALVDLRRHYDLILIDGPPLLSAAGSSVLGQTVDGVVVLI
                     ERGTSRDVIDQTRRQLDFLASDTLGYVFVHGS"
     gene            4810..5457
                     /locus_tag="PLP95_02850"
     CDS             4810..5457
                     /locus_tag="PLP95_02850"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005777 - peroxisome [Evidence IEA];
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003986 - acetyl-CoA hydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0008413 - 8-oxo-7,8-dihydroguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_function: GO:0010945 - CoA pyrophosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0035539 - 8-oxo-7,8-dihydrodeoxyguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0009132 - nucleoside diphosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0015938 - coenzyme A catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HPB44768.1"
                     /translation="MDLDDIAQRLTQMSIDRAMSMVAQSRQRSAVAVVLSQGRRGPEL
                     LLTRRAWHMRTHRGEIAFPGGGEEDGDVFPVGTALREACEEVALDPGSVEPIAALDPM
                     VTFTSDKAVVPIVFETDQRPSVVASPDEVDAILQVSFADLLEEGCYRQELWTWSDSTL
                     RDAPEAGYQHPVHFFELYGDTLWGATASMINQLLTVVFAPEAFEELMRGPEHFGR"
     gene            5559..5948
                     /locus_tag="PLP95_02855"
     CDS             5559..5948
                     /locus_tag="PLP95_02855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB44769.1"
                     /translation="MAMLTFEGETHGEIVVKVRRWLASLEEAPEGTLTAVEAIEQGAE
                     LTKDALRIIAAAAPAPVAQSELLKGLTGLGYKATDTTKEALVGGLDSIEEMTGGGVVR
                     QVQKAGVRAAWEMNSTVARQLLKSLRR"
     gene            6046..>6789
                     /locus_tag="PLP95_02860"
     CDS             6046..>6789
                     /locus_tag="PLP95_02860"
                     /inference="COORDINATES: protein motif:HMM:NF012690.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HPB44770.1"
                     /translation="MASIEIGRGKSARQGYELDDVSIIPSRRTRDADDVSTTWSIDAF
                     EFDVPLMTVDATSIPGGLAVLDAERFTNGDGSFDVEAIGEVLEEIDDDGEVSAIAVRP
                     QRAEELLEALGRTQPDLLVIKGRVVSAEHVSKSRDTLNLKKLVRSIEVPVIVGGCSSY
                     KAALHLMRTGATAVIVQSDLASEGVHVPLATAIADVRAARSRHLDETGVYTQIIAAVP
                     VRTGGDVAKAIACGADAVLVEAVGSDSAGV"
BASE COUNT         1059 a         2121 c         2518 g         1091 t
ORIGIN      
        1 gctccggaaa cgaggccgac ggcgaccgct cggccgacgt cagcggcgcg acggttcccg
       61 acgagaaccg gccggccctc gagccgtcgg acgaggcgga caccggcgcc gacaccggcg
      121 ccgacgacgc tgagtcgtcc accgtcaccg ccgcaccctc cgatcccgcc atcgacgccg
      181 tcgacgacag cgccgtaggc gagcgcgggg ccttggagga ggtggtctcg agcgcgaccg
      241 accccgtcac cgggaactcg gtgtcggacc tcgaagcggt tacctcgggc ctgcccgaga
      301 cgtgggatcg caacagtcgc ggggccgaga tccgcctcga cgaggccgcc cagttggcct
      361 gcgcgaacgc tcagatcggg ttggtccgac tcggcgagct gtcggcggat gccgccaccg
      421 gacacctcgc cgtcgccgcg gtgcgcgccg atcagagcgc ggtcgccgag atccacacct
      481 ccgcaaacgg attggcgctc gctgcgctca accactcaga ggcgggtgcc gccgaagcgt
      541 tcgtgcagat ctgtgtcgac cgcggctatg agggatgaca tgagtgccga gagtccggtc
      601 gacgtggcgg cggtgccgcc tgaaccgtct gcgctcgccc ggtatcgcgc cgacatcgcg
      661 gccctcgccg ctcatcagaa gagctcgcgc ggtgccccgc cgtattcgtt gctgatcaac
      721 cggccactcg ggcgtcgttt cgcagcgctc gcacacctgg tcggcctgac gccgaatcag
      781 gtttcgctca tcagcttcgg tttcacggcg gcgggcctcc tggtgttcgc cgtgatgtcg
      841 ccgagttggc cccagtcgat cctggccacc gccttgttgg tgttgggcta cgccatcgat
      901 tccgccgacg gtcagctcgc tcgcctcacc acgggtgggt cgctggcggg tgagtggctc
      961 gaccacacgc tcgacagcgt gaaactcggc gtctttcacg gggcgatcct ggtgtcggct
     1021 taccggttcg acgcagacct cggcgtgtgg ccgcaggtga ccgcgttggc attcggcatg
     1081 gttgcggcca cgttgtttct catcttcatc ctcaccgacc agcttcgccg tggcgccggt
     1141 ggcaccgccc ccgaacgtcc gcaggcctgg tggttcatgg tgctcaacgc cccgaccgac
     1201 tacggcgtgc agtgtctgtg gatggtcatg cggccgtcgt ccacggtgtt cttcggcgga
     1261 tatggcctgt tggccctcgc gaacctgggg tatctcgggg tgagcgggcg gagccggttc
     1321 cgccagatgc agcagatcga tcgagagcgg gtgcgacgat gacggtggtt ggctatgcgc
     1381 cgggggccta tgacctttta catgtcggtc acctccggct gctggaacgt tcgcgagagc
     1441 ggtgcgaccg gttggtggtc ggcgtcgtgg gcgacgacct tgccgaactc cagcgcgggg
     1501 agcgtccggc cgagccgctc gaagcgcgta tggcggcggt cgccgaactc gactgggtcg
     1561 acgaggtggt cgaggacccg tcgagcgaca aggcggtgat ctgggaacgg gttcgcttcg
     1621 acgtgatctt caagggcgac gactggcggg ggacccccaa gggggacgcg ctcgaggcgg
     1681 cgatggcccg cgtcggtgcc gaagtcgtgt atctgagcta caccaaggac atctcgagca
     1741 cccggttgcg gcgcgagctt cgcgaagcgt gttcgtgact ctcgctacac gctgacggtg
     1801 ttgccggcgc gctctcgtgt ggcgcgctcc cgtgcggcga gcggggtgag cgccatcgtt
     1861 ccctgagtgt tgcgttgggt gggtccgacc tcgcgttgca ggactccgac ccaggcgccg
     1921 aggatgagca gccacagcgc cgacacgcct actcccgacc cgacgccttc gacggtgaac
     1981 ccgttcacca tcagacacgc gagtaccccg ccgagcaacg gagcgtcgcc gtggcggagc
     2041 cgctggaggc gggcaattcc gagcccgatg gcgatgacga gaccgagcca cagcccgagg
     2101 ccgatgaggc cgccgtcgac cgcgacgttg atgaacgcgt tgtgggcccc gccgagtccg
     2161 acccggtcat agaagactcc gcgtgaggtc gacagccccc agccggtgag cggcttctgg
     2221 gagacgaggt cgacggcgat gccccacagt tcggtccggt tcgacacggt cgtgaggttc
     2281 tcggtgggtt cgccacgagc gaggtaggcg aggatgaccg gccagaagct gaacaccgcg
     2341 agcgccaggc ccacgaaccc caggacgccg agcggaaggc gctcccgcac cggcaccgtg
     2401 aggtaaatga ccacgaacac gccggcgatt gccgcgatga cggcgccccg ggtctgcgtg
     2461 gccaccaacg cggcacccat caccgcgaga catgtcagat agagcggccg tggccagcgc
     2521 tgggccccga gtcggaatcg gctgtggttg gtggccaggc cgagcgcgat ggtgatgccg
     2581 agggccaaca tcgcgccggc ggtgaccgag tggacataga gccagttgaa ccggtgggcc
     2641 tgcaggggcg agaacggcac gcggtagacg aggccgatga acaccgacag ggtgacgagg
     2701 ccgacatagg cgtgggccag ttgcagcacc tgacctcgcc gggcgttcgc cgcgatcgcg
     2761 ctcgagaacg cgaccatgat gcacaactgc atcgcccgaa ccgcgccgag gaccacatag
     2821 gagctgtaca tcaccgacat caccatggcg gcggtgtagc cgcgcatgaa gaactgcacg
     2881 gcggtggggc ggttccagcg cgggggtgtg acgacgtagc cgatgaggta cccggcgacg
     2941 agggcgaaca cggcgagctc gacgacgatc gccaggtcga ccgaggccga caacgagtcg
     3001 ttcagttcgc gctggcggaa cttgtactcg gttccgagga tcatggcgag gccgagcacc
     3061 gccggccagg tgtcggtcca gaggctgggg agtcgccagg ggagtcgcca ggggtgcgac
     3121 atcacgaacc gtggacgaag acgtagccga gcgtgtcgct ggcgaggaag tcgagctgac
     3181 gccgggtctg gtcgatgacg tcgcggctgg tgccgcgttc gatgagcacg acgactccgt
     3241 cgacggtttg gccgagcacc gaggatccgg ctgcgctcag cagcggcggt ccgtcgatga
     3301 ggatgaggtc gtagtggcgt cgcaggtcga cgagcgcgga ttcggtggcg ccggtgcgca
     3361 acaacgccgg gggctgggac ggatcgccgc cggggcccat caggaacagg gcggacgagg
     3421 tcatggcgct gttctcgcca aagcgcaggc gaacggttgc ctcgtcggcc tccgcgctgc
     3481 cggcgacgac gtcggcgaat ccgagacggt tgtcgagccc gaggatggtc gacacgccgc
     3541 gcgccccgtg gtcggcgtcg atgagcagca cgcgcttgcc ggcgcgggcg gcgacgagcg
     3601 cggtcttgat gacgacgtcg gcgtcgtggg ccatgaggtc ggcgggggtg aacaacacgg
     3661 tgtcgcccga caacaccgcg tcgatgttgg aggtgatccg ctgaaagctc ggctctggca
     3721 ccgcgaccat gtcgatggtc ttggcgctca cgaggtcgta gccgacctcg ccgagcatgg
     3781 gcaggcccag ccaggaggac acgtctgcgg agttctcgcc gatcggttcg cggtcggctc
     3841 ggaaccaggc gatcagcacc gcggcgagga agccgatgat gccggcgatc acgacgttgc
     3901 gcacgaggcg ggcgaggaag ttgctcgacg aggggacccg cgccgggtcg taggcgtcga
     3961 cgccgtcgcc gaacagtgcg cgggtggtgt tgatctccgc catgcgttgt tgaatctggc
     4021 cgtcgtcgga gaggtcgatg cctgggattc cggagtcggc acggggcgac tcgccgctga
     4081 gcgcggcgag cgctcgttcg gccttgtcgt cgaactggcg cgccgtggcg tcgcggtagg
     4141 cggcgacgat ggcgtcgacg gccccggtgg cggtgcggaa ctcgccgaac tggcaggtca
     4201 cggtcatgac cgtcgaggcg gaggctgcgg tggcggagca gttcgaccgc aggtcggctg
     4261 ccgagccggc ccgggggaag ttctgggcgg ccgcctggag cacgtcgctc gaggtggcga
     4321 acgcggcgcg ctccgcgctg tagcgggcga agtccaccac ggagatccca tcggcgaagg
     4381 tagcgctgcc gcgggggtcg gtgagggtga ccttgccgac cgcttcgaac gagttgggtc
     4441 gaagcgccgt gaagaccacg gcgatcagca cacatgcgag cggcaccgcg gtcatgagct
     4501 ttcggtagcg cagggcggct tcgatgatgc ccggggcagc ctgcggttgc gtcggttcgg
     4561 ttgcgaacat ggcgttccca tcggcgggtc ggggcgagat gtgaggggtg cggcgcccgt
     4621 tatggtggcg gtgctgtgga atcgttcgac ccgtcaccga aggcttcgac tggcccagca
     4681 cctggcccgt cgcccgagcc gtcgctcgag ccggcgcgcc gtggcggccc tcaggagatc
     4741 cctcgtccga cctcggcgcg tctcggcgct gctccggcgt ggtcgggtgc catcggtagt
     4801 gtcccgtcgt tggacctcga cgacatcgca caacgattga cgcagatgtc gatcgatcgg
     4861 gcgatgtcga tggtggcgca gtcccgacag cgcagtgcgg ttgcggtcgt gttgtcacag
     4921 gggcggcggg ggccggaact gttgttgacc cggcgtgcct ggcacatgcg cacccatcgt
     4981 ggcgagatcg cctttcccgg tggcggcgag gaggacggcg acgtctttcc ggtgggcacg
     5041 gcgctgcgcg aggcgtgtga ggaggtggcg ctcgatcccg gttcggtcga accgatcgcg
     5101 gcgctcgacc cgatggtgac gttcacgagc gacaaggccg tcgtaccgat cgtgttcgag
     5161 accgaccagc gccccagcgt cgtggccagc cccgatgagg tcgacgccat cttgcaggtg
     5221 agcttcgccg acctgctcga ggaggggtgt tatcgccagg agttgtggac ctggagcgat
     5281 tccacgcttc gcgacgcccc cgaggcgggg tatcaacatc ccgttcactt cttcgagctc
     5341 tacggcgaca cgttgtgggg tgcgacggct tcgatgatca atcagctgtt gacggtcgtg
     5401 ttcgccccgg aggcgttcga ggagttgatg cgaggccccg agcacttcgg gcgctgaagg
     5461 tcgggcggcg cgcgtcggga cgcgttcggc aggacgcgtt cggcaggacg cgttcggcgg
     5521 ttcggccgct atcgtctgat ccacagggag gtgtgccgat ggcgatgttg acctttgaag
     5581 gtgagaccca cggcgagatc gtggtgaagg ttcggcgctg gttggcgtcg ctcgaggagg
     5641 ccccggaagg gaccctgaca gcggttgagg cgatcgagca gggtgctgaa ctcaccaagg
     5701 acgcgctgcg catcatcgcg gcggccgcgc cggctccggt tgcgcagagc gagttgctga
     5761 aaggcctgac cgggctcggc tacaaggcga ccgacacgac caaggaagcg ctcgtcggcg
     5821 gactcgactc gatcgaggag atgaccggtg gtggcgtcgt gcgtcaggtg caaaaggccg
     5881 gggtgcgggc ggcgtgggag atgaactcga ccgtggcgcg tcagttgttg aagtcgcttc
     5941 gacgctgatc tgttgaacgg ttcgccccgc tgtggatgag ggcgaaaccc gaggcctaga
     6001 ctggcgcctt ttccggccgc ggagtgccgg gtgaaggaca tggtcatggc atcaatcgaa
     6061 atcggacgcg gcaagtcggc gcggcagggc tacgaactcg acgacgtgtc gatcatcccg
     6121 tcgcgacgca cccgcgacgc tgatgacgtc tccaccacct ggagcatcga tgcgttcgag
     6181 ttcgacgttc cgttgatgac cgtcgacgcg acgtcgattc ccggcgggtt ggcggtgctc
     6241 gatgccgagc ggttcaccaa cggcgacggt tccttcgacg tcgaggccat cggcgaggtg
     6301 ctcgaggaga tcgatgacga cggcgaggtg tcggcgattg cggtccgccc ccagcgcgcc
     6361 gaggagttgc tcgaagcgct cggccgcacc caacccgacc tgttggtcat caagggtcgg
     6421 gtggtgtctg ccgagcacgt gtcgaagtcc cgcgacaccc tcaacctgaa gaagctggtg
     6481 cgttcgatcg aggtgccggt catcgtcggc ggttgttcct cctacaaggc ggcgttgcac
     6541 ctcatgcgaa ccggggcgac cgcggtgatc gtgcagagcg acctggcctc ggagggcgtg
     6601 cacgttccgc tcgccacggc gatcgccgat gtgcgcgccg cccgctcgcg ccacctcgac
     6661 gaaaccgggg tgtacaccca gatcatcgcg gcggtgccgg ttcgtaccgg cggcgatgtg
     6721 gccaaggcga ttgcgtgcgg ggccgacgcg gtgctcgtcg aggcggtggg aagcgactcg
     6781 gcgggggtg
//
LOCUS       DAOOMA010000060         6762 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_13408_length_6762_cov_4.798718, whole genome shotgun sequence.
ACCESSION   DAOOMA010000060 DAOOMA010000000
VERSION     DAOOMA010000060.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 6762)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6762)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6762
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13408_length_6762_cov_4.798718"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(59..736)
                     /locus_tag="PLP95_02865"
     CDS             complement(59..736)
                     /locus_tag="PLP95_02865"
                     /inference="COORDINATES: protein motif:HMM:NF020893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HPB44771.1"
                     /translation="MTLAVMTLLAAIPGLPEQLDPKWLIQNGGPWLLFAIIFAESGLL
                     IGFFLPGDSLLFTAGAVAAGVFTDWGVEFNIYMIVIGCFIAAVTGDQVGYLIGNKAGP
                     KLFNRPDSRLFKQEYVNKGTEFFQKYGGRAIVLARFVPIVRTFVPTIAGVSNMQYVDF
                     LKYNVVGGLLWAVGVTMLGYFFGQISFVNENIEIALILIVFISILPILFEFQKARKEK
                     RAEAAEA"
     gene            complement(827..3745)
                     /locus_tag="PLP95_02870"
     CDS             complement(827..3745)
                     /locus_tag="PLP95_02870"
                     /inference="COORDINATES: protein motif:HMM:NF015644.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0182 family protein"
                     /protein_id="HPB44772.1"
                     /translation="MLFVFVGLVVLALVFSRGVAGVYTDYLWFRALGVASEWQRQWGY
                     RTILGLLFGLGFFVLLGGNLLVANRIAPLLTPRRTGDELVERYRELIDGRQRLVWLSA
                     SLLFSALAGIGASSQWRNWVLFRFGGTFGQVDAEHQVDLSFYVFKLPFLSYSVSWLFA
                     ALVIVTLFTVMAHYLTGALQLPGTGRVATGRVKIHLSVLFASLALVKALDYLLERYRL
                     TLSRRGAVAGALYTEANVTVRSMSLLALVAVACAVVLVLNIRRRGWGIPVITIGLWLI
                     VSLVAGIILPAAMQRLKVSPNESALEAEYIARNIAATRNSLGLDAVREHDFDYSPTLK
                     DEEITANASTVSNVRLLDPQVVQPTFTSLQSMFEYYRFADLDVDRYDIGGAQTLVVIG
                     ARELSQSSLPVNTWEARHLSYTHGYGAAVAQANGVNSEGEPDFLVGSIPPQIDSEQLP
                     SVSIDRPEIYFGEGFDRSDAGGYAIVGTDREEQTDGGPGPGGVDVGGVIRRAAFALRF
                     GDFEMLTSSYLDAESRVVYRRNVSERVAAIAPFITWDNDPYPVLVDGGISFVLDGYTT
                     TSWIPYAEPATTADLESSAPLRSEDFNYLRNSVKAVVDAYDGSVTLYLSDELYGDHDP
                     IVRAYAAAFPGLFTPLGEMSDELRSHLRYPEDLFRIQTQMWGRYHLDDPAEFYSQQDG
                     WDVAQNPPNDTGRDSSTTTTTTGTSPIAPNYLQMRLPGESADEFVMLRPFVPHSGASQ
                     SSPKKQLNAFMVARSDPDDYGTLSVYTMTKQGADGSVQRTNEVPSPLFAHENMVSTPM
                     LSQRLAQLNSSSGSSVVTFGNMVMVPIESGILYVRPIYVASETRGSAQQLRIVVVSVG
                     TRVGVGDTYAEALSSLFPTAEITTAEEGAAGEGADPGDGETSGGDAPGIDEADAGVLL
                     AEAVARYDEADAILRAGGATALADYQDAIAEAEELVRRAAGLLSGIPAASQEASGSSG
                     "
     gene            complement(3822..6575)
                     /locus_tag="PLP95_02875"
     CDS             complement(3822..6575)
                     /locus_tag="PLP95_02875"
                     /inference="COORDINATES: protein motif:HMM:NF016959.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HPB44773.1"
                     /translation="MTLSGDAFHIDPGEAATREFSRARKGYDPTEVRVHITRLTDEIR
                     RLRGVETALETQVELARSQALDLESLDPATISRALGEETVRVIDAAREAATEIRSKAE
                     ENAGRLVREAQERASELTREAEELRARSEREAQELREDVVAEVEALRTSTVQECEAQR
                     AEIDEYVEHRRRDIEAQAELADQNAEQVLEEAKSDLADARAEAAEIRAGAEADAESIR
                     REAEEARARRESEATEDADRIRDAAEAAAAAIREEAEVVRAAAEEDVAERLNTARADA
                     EAEVERARAVARSMIEETQLVRERMIRDLAERRHGARQQLEATRAGRERLLAVFDQAR
                     ASFDEIVEEVVEALPLARHDADAAARALPNDTYTSVEQLDRLIHAGASNDELFEGALA
                     DGGEAPEVDFLAAAGLASSDGSSMAQIDIELVLDDDDDDDDQDEDVIGGDEVDEVAVA
                     SAESSLVAEVDAELVGDEITEQVVEELAEAFVEVDAATVDADGEAGAGEETFEAEADR
                     DVALADEHDDLDDELDDEMVDGEIVDGEIVDDLALSDEDEDEDEDPSFGRLRLVSSNQ
                     DGPSPVGIELLTDSEADSTADVGDEPAGEGEPGAVDAIFALLRADHDVDDDDDFDDDD
                     GDYLGQLDSDDPDVASDDVAALAAVASINGPAMVIDLAAAEQRLEQSEAAVAVAVLTT
                     EQDRTLAQQALLDERDDAIGDLGQQIARRIRRVVSDQENEVLDLIRRNKKMKTAEQIL
                     PSAVEQMAAYRSAVAADLTAVLHAGVEFALDTWRPETHVDQIDVSAAFDQAVERLSEL
                     AVAPLTAKLARIIAEVDDPTPDRTDLVDAVRLGYREFKNDRIAEVAGDLVTVAFSRGV
                     LAAAEPGDEVRWIVDHSGRACPDGEDNNLAGPTAAGEAFPTGDDCPPVHPGCRCLLVP
                     AAR"
BASE COUNT         1186 a         2269 c         2128 g         1179 t
ORIGIN      
        1 cgataaccag tggtcgtgat cacgaccact ggttatcgga gaacgggcgg ggttgcggtc
       61 aggcctcagc cgcctcggcg cgcttttcct tgcgagcctt ctggaactcg aacaggatcg
      121 gcaggatcga gatgaacacg atcaggatga gggcgatctc gatgttttcg ttgacgaagc
      181 tgatctggcc gaagaagtag ccgagcatcg tcaccccgac cgcccacagc aggcccccga
      241 cgacgttgta tttcaggaag tccacgtatt gcatgttcga cacgccggcg atcgtcggca
      301 cgaacgtacg cacgatcggc acgaaccggg ccaacacgat ggcccggcca ccgtacttct
      361 ggaagaactc ggtgcccttg tttacgtact cctgtttgaa gagccgtgaa tcaggccggt
      421 tgaacagctt cgggccggcc ttgttgccga tgaggtaacc gacttggtcg cccgtcaccg
      481 cagcgatgaa gcagccgatc acgatcatgt agatattgaa ttcgacaccc cagtcggtga
      541 agacaccggc ggccaccgca ccggcggtga acaacagcga gtcacccggc aggaagaagc
      601 cgatcagcaa ccccgactcg gcgaagatga tcgcaaacaa cagccagggt ccgccgttct
      661 ggatcaacca tttcggatcg agttgctccg ggagccccgg aatcgcagcg agcaaggtca
      721 tcacggccaa tgtcacggcg cagaccctag ttggccgtac ccgtcacggc gatgtcaggc
      781 ccgacgatca ggcccgacga tcaggcccga cgatcaggcc cgacgatcaa cccgacgagc
      841 ccgacgcctc ctgcgacgct gcggggatcc cgctcaacaa cccggcggcg cggcgaacga
      901 gttcctcggc ctcggcgatg gcgtcttgat aatcggcaag tgcggtggct ccgccggcgc
      961 gaaggatggc gtccgcctcg tcgtagcgag cgaccgcctc ggccaacagc acgccggcgt
     1021 cagcctcatc gatgcctgga gcatcgccgc cggaggtttc tccgtcgccc gggtctgccc
     1081 cttcaccagc agctccctct tcagcggtgg tgatttccgc cgtcggaaac aacgaggaca
     1141 gcgcttcggc gtaggtgtcg cccaccccga cccgggttcc gaccgagacc accacgatcc
     1201 gcaactgttg tgccgagccc cgcgtctcgc tcgcgacgta gatcgggcgg acatacaaaa
     1261 tccccgactc gatgggcacc atgaccatgt tgccgaaggt caccaccgaa ctgccgctgc
     1321 tcgagttcag ttgggcgagc cgctgagaca gcatgggggt cgacaccatg ttctcgtgcg
     1381 cgaaaagtgg gctcggtacc tcgttggtgc gctgaaccga accgtctgca ccctgcttgg
     1441 tcatcgtgta caccgacagc gtcccgtagt cgtcggggtc gctgcgcgcc accatgaacg
     1501 cgttgagctg ctttttcgga ctggactgcg atgccccaga atgcgggacg aacggccgca
     1561 gcatgacgaa ctcatcggcc gactcgccgg gtaggcgcat ctgcagatag ttcggagcga
     1621 tcggagatgt tcccgtcgtc gtggtggtcg tggagctgtc gcggcccgtg tcgttcggcg
     1681 ggttctgagc gacgtcccat ccgtcttgtt ggctgtagaa ctcggcgggg tcgtcgaggt
     1741 gatagcgacc ccacatctgg gtttggatcc ggaacagatc ctcggggtag cgaaggtgcg
     1801 accgaagttc gtcgctcatt tcccccagcg gggtgaacag gccgggaaag gccgccgcgt
     1861 acgctcgcac gatcgggtcg tggtcgccgt acagttcgtc ggagagatac agggtgaccg
     1921 aaccgtcgta cgcgtccacc acggctttga ccgagttgcg caagtagttg aaatcctccg
     1981 agcgcaaggg tgcggaggac tcgaggtcgg cggtcgtcgc cggttcggcg tagggaatcc
     2041 acgatgtcgt cgtgtacccg tcgagcacga agctgatgcc gccatcgacc agaaccgggt
     2101 aggggtcgtt gtcccacgtg atgaacggag cgatcgcagc aacccgttcg gagacgtttc
     2161 ggcggtacac gaccctcgat tcggcgtcga ggtagctgga ggtcagcatc tcgaaatccc
     2221 cgaacctcaa cgcgaacgcg gcacgccgaa tcacgccccc gacatcgaca cctcccggtc
     2281 cggggccacc gtcggtctgc tcttcacggt cggttccgac gatcgcatat ccgcccgcat
     2341 cggaccggtc gaacccttca ccgaagtaga tttcggggcg atcgatggaa accgacggca
     2401 gttgctcgga atcgatctgg ggcgggatgc ttccgacgag aaaatcgggt tcgccctcgc
     2461 tgttgacccc gttggcctga gcgaccgcgg cgccgtagcc atgggtgtag gacagatgac
     2521 gggcttccca cgtgttgacc ggcaacgacg actggctgag ctcccgagca ccgatcacca
     2581 ccagggtttg ggcaccaccg atgtcgtaac ggtcgacgtc gaggtcggcg aaccggtagt
     2641 actcgaacat cgactgaagg ctggtgaagg tcggttgaac cacctgcggg tcgaggaggc
     2701 gcacgttgga aacggtcgac gcgttggccg tgatctcctc atccttgagc gtcggcgagt
     2761 agtcgaagtc gtgctcccgc accgcgtcga gccccagcga gttgcgcgtc gcagcgatgt
     2821 tcctcgcgat gtattccgcc tcgagcgccg actcgttcgg gctcaccttc agacgctgca
     2881 tcgccgccgg caagatgatt cccgccacga gcgacacgat gagccacagg ccgatggtga
     2941 tcaccggaat cccccagccg cgccgtcgga tgttcaacac gagcaccact gcgcacgcca
     3001 cggcaacgag cgccaacagc gacatcgaac gaaccgtcac attggcttcg gtgtacaggg
     3061 caccggccac cgcaccgcgc cgcgacaggg tgagccggta gcgctccagc aggtaatcga
     3121 gcgccttgac cagcgcgagc gatgcgaaca gcaccgacag gtggatcttc acccgaccgg
     3181 tcgcgacgcg accggttccc ggcagttgca gcgctcccgt caggtaatgg gccatgaccg
     3241 tgaacagcgt gacgatgacg agtgcagcga acagccagct caccgaatag gacaggaacg
     3301 ggagcttgaa gacgtaaaac gacaggtcga cctgatgttc tgcgtcgacc tggccgaacg
     3361 ttccgccgaa gcgaaacagc acccaattgc gccattgcga cgacgccccg atgcctgcga
     3421 gcgccgagaa cagcagtgag gccgacaacc acacgagacg ctggcggccg tcgatgagct
     3481 cgcggtagcg ctcgacgagt tcgtcgcccg ttcgccgcgg ggtgagcaac ggagcgatcc
     3541 ggttggccac caacagattg ccgccgagca acacgaagaa cccgaggccg aacagcagcc
     3601 cgaggatggt ccggtacccc cattgccgct gccattccga ggcaacaccg agagcgcgaa
     3661 accacagata gtcggtgtag acaccggcga cgccgcgcga aaacacgagc gcgagtacca
     3721 ccagtccgac gaagacgaac agcaccgtcc gccctcgcga acggcccacc tcgctgtttg
     3781 cgcgagtcca gcgaagtgac cgcttgagca tcccgccgat gttaccgagc ggcgggcacc
     3841 aacaagcagc gacaccctgg atgcacgggc ggacagtcgt ctccggtcgg gaatgcttcg
     3901 cctgctgcgg tcggcccggc gaggttgttg tcctcgccgt cgggacacgc gcggcccgag
     3961 tgatcgacga tccaacgcac ttcatcgccc ggctctgcgg ccgcgagcac gccacggctg
     4021 aacgcaacgg tcacgagatc gccggcaact tctgcgattc ggtcgttctt gaactcgcgg
     4081 tagccgaggc gtacggcgtc gacgaggtcg gtgcggtccg gtgtcggatc gtcgacttcg
     4141 gcgatgatcc tggcgagctt cgccgtcagg ggggccaccg cgagctcgga gagtcgctcg
     4201 acggcctgat cgaacgctgc ggaaacgtcg atctggtcga cgtgggtttc tgggcgccac
     4261 gtatcgagag cgaactcgac ccctgcgtgc aacacggcgg tgagatcggc ggcgaccgcc
     4321 gaccggtacg ccgccatctg ctcgaccgca ctcggcagaa tctgctcggc ggtcttcatc
     4381 ttcttgttgc gacgaatgag gtcgagcacc tcgttctcct ggtcggacac gacccggcgg
     4441 attcgacgtg cgatctgttg accgaggtcg ccgatcgcgt cgtcgcgctc gtcgagcagg
     4501 gcctgttgag caagcgtgcg gtcctgctcg gtggtgagca ccgccaccgc caccgccgct
     4561 tcggactgtt cgaggcgttg ctcggcagcg gccaagtcga tcaccatcgc cggcccgttg
     4621 atcgatgcca ccgcggcgag cgccgccaca tcatcggatg cgacgtcggg gtcatcgctg
     4681 tcgagttgac cgaggtagtc gccatcgtcg tcatcgaagt cgtcgtcatc gtcgacgtcg
     4741 tgatcggcgc gcaacagggc gaagatcgcg tcgaccgcgc cgggttcgcc ttccccggcg
     4801 ggttcgtcgc cgacgtcggc ggtggagtcg gcctcgctgt cggtgagcaa ctcgatcccg
     4861 accggtgatg ggccgtcctg attcgagctc acaagccgaa ggcgaccgaa cgaggggtct
     4921 tcatcctcgt cttcgtcctc gtcactcaag gcaaggtcgt cgacgatctc gccgtcgacg
     4981 atctcgccgt cgaccatctc gtcgtcaagc tcgtcgtcga ggtcgtcgtg ttcgtcggcc
     5041 agagccacat cacggtcggc ttcagcctcg aacgtctcct ctcccgcccc ggcttcgcca
     5101 tccgcatcaa cggtcgcagc gtcgacctca acaaacgcct cggcaagctc ctcgacaacc
     5161 tgttcggtga tctcatctcc cacgagttcg gcatcgacct ccgcaacgag cgacgactcg
     5221 gcgctcgcta cggcgacctc gtcgacctcg tcgccaccga tgacgtcttc atcctgatcg
     5281 tcatcgtcat cgtcgtcgtc gagcacgagt tcgatgtcga tctgtgccat cgaggaaccg
     5341 tcggacgatg ccagcccggc cgccgccagg aaatccacct cgggcgcttc gccgccatcg
     5401 gccagggcgc cttcgaacaa ctcgtcgttg ctcgcaccgg catggatcag tcgatcgagc
     5461 tgctcgacgg aggtgtaggt gtcattcggc agtgcccggg ctgctgcgtc ggcgtcgtgg
     5521 cgggccagag gcagcgcctc gaccacctcc tcgacgatct cgtcgaagga agcacgcgcc
     5581 tggtcgaaca cggccaacaa tcgttcccgc cccgcccgcg tcgcctccaa ctgctggcgc
     5641 gccccgtgac ggcgctccgc gaggtcgcgg atcatccgct ctcgcacgag ctgggtttcc
     5701 tcgatcatgg aacgtgcgac cgctcgcgcc cgctcgacct cggcttcggc atcggcccgt
     5761 gccgtgttca gccgttccgc aacgtcctcc tcggcggccg cacgtacgac ctcggcctcc
     5821 tcgcggatcg cagccgccgc ggcctctgcg gcatcgcgaa tccgatccgc gtcctcggtg
     5881 gcctcggact cacggcgcgc ccgagcctcc tcggcctcgc gccggatcga ctcggcatcc
     5941 gcctcggctc cggctcggat ctcggcggct tcagcccgcg cgtccgccag atccgacttg
     6001 gcctcttcga gcacctgctc ggcgttctgg tcggccagct ccgcctgagc ttcgatgtcg
     6061 cgtcgccggt gctcgacgta ctcgtcgatt tcggcccgct gcgcctcgca ttcctgaacg
     6121 gtgctcgtgc gcagcgcctc gacctcggcc acgacgtcct cgcgaagctc ctgcgcttct
     6181 cgttcggatc gggcccgcaa ctcctcggcc tcgcgggtca gttcgcttgc acgctcctgt
     6241 gcctcacgaa cgaggcgccc tgcgttctcc tcggctttcg agcggatctc ggtcgcagct
     6301 tcgcgagcag cgtcgatgac gcgcacggtt tcctcaccga gggcacggct gatcgtcgcc
     6361 gggtcgagcg actcgaggtc gagcgcctgg ctccgagcga gttcgacctg ggtttccaac
     6421 gccgtttcaa cgccgcgaag ccgccggatc tcgtcggtga ggcgagtgat atgaacccgc
     6481 acctcggtcg ggtcataccc cttgcgcgcc cgtgagaact cacgggtggc ggcttcgccg
     6541 gggtcgatgt gaaaggcgtc accggacagc gtcatgtcga cgatgctacg accgcacgcg
     6601 gtggttcagg tggacaccgc cgacatgttt ttcgagccga tcagttctcg gcggcgtcgc
     6661 cgtattccgc ggggtcgagt agtgcgaggg cctcgcgcag cgtcgacacg gcatggagct
     6721 cgatgcgtcc aatgttcaca gcctctcact caacatggag gt
//
LOCUS       DAOOMA010000061         6746 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_13461_length_6746_cov_4.581378, whole genome shotgun sequence.
ACCESSION   DAOOMA010000061 DAOOMA010000000
VERSION     DAOOMA010000061.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 6746)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6746)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6746
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13461_length_6746_cov_4.581378"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..234)
                     /locus_tag="PLP95_02880"
     CDS             complement(<1..234)
                     /locus_tag="PLP95_02880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB44774.1"
                     /translation="MAPRVASLRSSFIRDLLASSCSPGVLSLAGGLPGDDLIPVEAVD
                     RAARRVLAGGSAALNALQYGATEGAAELRDVLGE"
     gene            413..1234
                     /locus_tag="PLP95_02885"
     CDS             413..1234
                     /locus_tag="PLP95_02885"
                     /inference="COORDINATES: protein motif:HMM:NF037627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HPB44775.1"
                     /translation="MTSDFELGDYLRSLRESTQPSTVGLGHRGRRRTPGLRREEVAML
                     AGVSVSWYTWLEQGRPIKASVEVLDALARVFGLDGAQRDHLVELAGHRTRPTGSPIGV
                     DVPDWAQRLLDSIDPIPAYLLGPTWEYLGWNRAQAELFPPITTLAENERNLVWVLFAL
                     PEVRDLIVGWEHEARRSLAQFRADIAPWRDDPAVHDLVERLRRVSPEFDEWWDRHDVA
                     GFETRIRRFNHPTLGVLGVEYQQLIPAGRSDLRVVMQLPIADASLDSGVSDPPTG"
     gene            complement(1212..2030)
                     /locus_tag="PLP95_02890"
     CDS             complement(1212..2030)
                     /locus_tag="PLP95_02890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB44776.1"
                     /translation="MAVALVVGAAATIAGFPAEQASADTTTGGASTDSGAAVGISKRR
                     APTKSNRRYSTRCDIYEVLELNPSNPKGPRGPRLNRNTVLPGTLVWQTCRDIRSNAIV
                     SGPRLVPLRAPGGTQLSLATVDVPDPAIQFMPPESTLPGVLTRLDLSGPMIVSAQASI
                     GVETVTVYAALVDTTFAVESTTFVCEAPGWFAETQDIKPCTHRFAGPRRELTIDVTAR
                     WAMWWVDVSGAIELLPDHVRRVQVPYAVGSATTRVAGTGWVERWMAGVTRSEDR"
     gene            complement(2099..2848)
                     /locus_tag="PLP95_02895"
     CDS             complement(2099..2848)
                     /locus_tag="PLP95_02895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB44777.1"
                     /translation="MLLCSGSRPASRPGGRTMPKRLAIAWAAVFLAAVFLVAGCGGEP
                     SARNGDADGLVPVAGSDAADRPTSTLAGPASTSTTRVDDTALDDTAPGDDTTVTLPAR
                     EAEVAPEHVFVVAANGLAWQEALDALAALDPSAVNASGKMTEDLAAAWVDEIERWREA
                     GAVRTEERRASWRRVESVIEVEPGVVEIEACQFAGLAMYGADDAVVWPGNAAASREVA
                     VMVSTPDGWRWDDTYLDSADGTSPVNDCAWP"
     gene            2995..3690
                     /locus_tag="PLP95_02900"
     CDS             2995..3690
                     /locus_tag="PLP95_02900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB44778.1"
                     /translation="MRTLVLSPHFDDAPLSLGQSMLDGRLAGERVTVGVVFSHSNWTR
                     WFHPTRRRWPLATAIRLSEESLASIRLRYRLRLGGFEETVLRSGDLSPSTYLVRDPQI
                     GDDPIVDAVERVVRDWAQGAQRIIAPLGVGDHRDHQIVTEAARRLLVDGLRVEFYEDR
                     PYVTWAAHGEAERIAALVDDRLERTAVSGPISEAKHRRLFYPSQFTEEFRGAFAADES
                     GADGEFVWALPNA"
     gene            complement(3702..4469)
                     /locus_tag="PLP95_02905"
     CDS             complement(3702..4469)
                     /locus_tag="PLP95_02905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020378060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HPB44779.1"
                     /translation="MQSESKQFDGKVSIVTGAGQGIGEEYAHALAREGAAVVVADLNE
                     ENANRVAADIVADGGRALGVKVDVSDPESCVAMAEAAVAEFGRIDHLVNNAAIYHGME
                     INGLLRIDLDYYRKFMDVNMNGAVYATRACWREMRKNEGAAVVNQTSTAAWMASGFYG
                     VAKAALNSITINLAAEMASLGIRVNAIAPGPTNTAATQILTGGEENFDILLTDVMIKR
                     PGTPLDMANALVFLLSDKASWITGQIIAVDGGQSKRI"
     gene            complement(4491..4877)
                     /locus_tag="PLP95_02910"
     CDS             complement(4491..4877)
                     /locus_tag="PLP95_02910"
                     /inference="COORDINATES: protein motif:HMM:NF014664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HPB44780.1"
                     /translation="MTDHVEPSPERSANLQRMGEVYGWEMTDGTGDFFSYTVDHLFGE
                     IWNRPGLDDRARRLLLIGLLIGSGQEDVLDIQFPAALSSGDLSTEELREIVILACHYA
                     GWPRGGRINTQAEKAIHQFEKKQQAS"
     gene            complement(4874..5737)
                     /locus_tag="PLP95_02915"
     CDS             complement(4874..5737)
                     /locus_tag="PLP95_02915"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015411.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HPB44781.1"
                     /translation="MAVAGFVGLGNIGAPMAKRLLSNPDGLAVFDLSAEATAPFAEAG
                     ATVADPLAALGEAAEVICICVRDDAQVREVVGGLLEVARPGQAYVIHSTIRATTAEDL
                     AEIAAAKGVYVVDAPISGGAMGAGDGRLAIMVGGTDEALEVARPVLELMGSMISHLGP
                     VGAGTRTKLARNMMHFVSYASALEASRLAEAAGIDIEELGRIVRHSDQVTGGPGAIQV
                     RNTTAQIDPDDWWYGVMENVLSLGGKDLSQAIELADELGVDVPIARLGLDQLAFALGV
                     ASSPLNTGRNQ"
     gene            complement(5737..>6746)
                     /locus_tag="PLP95_02920"
     CDS             complement(5737..>6746)
                     /locus_tag="PLP95_02920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020378061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HPB44782.1"
                     /translation="PWNFPHQINLAKVIPALAAGCTVVLKAAPATPWAANNLARLVAE
                     HTDMPAGVFNVITSQENAIGEVLCTDPRVDMVSFTGSTATGRKVMAAASATLKKVFLE
                     LGGKSAFIALDDADPMAVAMGAGFQIMAHAGQGCAITTRLLVPRARQDEFVDALVSMM
                     QGIGWGDPTDPGNFMGPIVSEVQRTKVLDYINTAVDDGAELVLGSGKPAVLEGELAGG
                     YFVEPTVLVNVDQNSRIAQEEVFGPVLAVIAHDGDDDAVAIANNSLYGLSGAVTSNDI
                     ERARSVARRIRTGTISVNGGLYYSPDAPFGGYKQSGIGREMGVAGFEEYLELKTIAEG
                     I"
BASE COUNT         1063 a         2289 c         2266 g         1128 t
ORIGIN      
        1 ctcccccaac acgtcgcgaa gctcggccgc gccctcggtg gcgccgtact gcaacgcgtt
       61 cagcgccgcg gaccccccgg cgagcacccg cctcgctgct cgatcaaccg cttcgaccgg
      121 aatgaggtcg tctccgggca ggccccccgc caacgacagc acccccggcg aacagctcga
      181 cgccagcagg tcacggatga acgacgaacg cagcgaggcc acccgtggcg ccatcagcga
      241 aggcgaaggt gagcgtggag gtaagggcga gcatcgggtc agcgtggtca tggctgaatc
      301 agaccaggag tccggcgcct cgaacagtga gcgtcgatcc tgtgatgtcc accaccaccc
      361 tcggcgcagt atcggcgcgg gtcggcgcgg ttcgatgtgg ttcgatgtgg tcgtgacttc
      421 ggatttcgaa ctcggcgact acctgcgttc gttgcgcgaa tcgacccagc cgtcgacggt
      481 cgggctcggc caccggggcc gccgccgtac ccctggccta cgacgcgagg aggtggcgat
      541 gctcgccggc gtgtcggtga gttggtacac ctggctcgaa caagggcgcc cgatcaaggc
      601 atcggtcgag gtgctcgacg cgctcgcccg cgtgttcgga ctcgacggcg cccaacgcga
      661 tcatctcgtc gagttggccg ggcaccggac ccgacccacc ggctctccga tcggggtcga
      721 cgtgccggac tgggcgcagc ggctcctcga ctcgatcgac ccgatccccg cctacctgct
      781 cggaccgacg tgggagtacc tgggttggaa ccgcgcccaa gcggagctgt tcccgccgat
      841 caccacgctc gcggagaatg aacggaacct ggtgtgggtc ttgttcgcgc tccccgaggt
      901 gcgcgacctc atcgtggggt gggaacacga ggcgcgtcgt tccctggcgc agttccgcgc
      961 ggatatcgca ccgtggcgcg acgacccggc ggtccacgac ctcgtcgaac gtctccgtcg
     1021 cgtgagcccg gaattcgacg agtggtggga ccgacacgac gtcgccggat tcgaaaccag
     1081 gatccgacgg ttcaaccatc caaccctcgg agtgctcggc gtcgagtacc aacagctcat
     1141 tcccgcgggg cgaagcgacc tccgggtggt catgcaattg ccgatcgccg acgcgtcgct
     1201 cgactccgga gtcagcgatc ctccgaccgg gtgaccccgg ccatccaccg ttcgacccag
     1261 ccggtgcccg ccacccgagt tgtcgccgaa ccgaccgcat acggcacctg cacccggcgg
     1321 acatggtcgg gcagcaattc gatcgccccc gagacgtcga cccaccacat cgcccagcgg
     1381 gcggtcacgt cgatcgtcag ttcgcggcgg ggtccggcga accgatgggt gcacggcttg
     1441 atgtcctgtg tctcggcgaa ccaaccgggg gcctcgcaca cgaaggtcgt cgactcgacg
     1501 gcgaaggtcg tatcgacgag ggcggcgtac acggtcacgg tctcgacgcc gatggaggcc
     1561 tgagcactca cgatcatcgg acccgacagg tcgaggcgcg tgagcacccc gggcagggtc
     1621 gactccggcg gcatgaactg gatggccgga tcgggaacgt cgacggtcgc caacgagagc
     1681 tgggttccgc ccggggcgcg cagcggcacg agacgcggac ccgaaacgat ggcgttgctg
     1741 cggatgtctc gacaggtctg ccacaccagg gtccccggaa ggaccgtgtt gcgattcagg
     1801 cgcgggcctc gtgggccttt ggggttgctc ggattcaatt cgagcacctc atagatgtcg
     1861 caccgggtcg agtatcggcg gttcgatttc gtgggagcgc gacgtttcga gatcccgacc
     1921 gctgccccgc tgtcggttga ggcgccgccg gtggtcgtgt cggccgacgc ctgttcggcg
     1981 gggaaccctg cgatggtcgc ggcggcgccg accacgagtg cgaccgccac aagcgcgacc
     2041 aaaccaacac gcgtccgccc cgaagtgcgc gacgacgggc gcctgccgag tcgccgaatc
     2101 acggccacgc gcaatcgttc accggcgagg tgccgtccgc cgagtcgagg taggtgtcgt
     2161 cccacctcca gccgtcgggc gtcgacacca tcacggccac ctcgcgcgac gccgcggcgt
     2221 tgcccggcca cacgaccgcg tcatcagcgc cgtacatggc gagcccggcg aactggcagg
     2281 cctcgatttc caccaccccg ggttccacct cgatcaccga ctcaacccgt cgccacgaag
     2341 cgcgtcgttc ctcggtcctg accgcgccgg cttcgcgcca ccgttcgatc tcgtccaccc
     2401 aggccgcagc cagatcctcg gtcatcttcc cgctcgcgtt caccgccgac ggatcgaggg
     2461 ccgcgagcgc gtcgagcgct tcctgccagg ccaacccatt ggcggccacg acgaagacgt
     2521 gctccggcgc gacttcagcc tcgcgcgctg gcagggtcac cgtcgtatcg tcgccgggcg
     2581 ccgtgtcgtc gagcgccgtg tcgtcgacgc gcgtggtgga agtcgacgcc ggtcccgcga
     2641 gcgtcgacgt gggacggtcg gcggcatccg acccagccac aggaacgagt ccatcggcat
     2701 cgccgttgcg ggccgagggt tcgccgccgc agccggccac caggaatacc gccgccagga
     2761 ataccgcagc ccaggcgatc gccaaccgct ttggcatggt gcggccaccg ggacgcgagg
     2821 cggggcgaga tccgctgcac aacaacacgc tggttgacgc tacggatcga agtgcacgcg
     2881 acccataggt gtccgggacc agtggcgatg ggtcgatccc ccatcgctgg tccggggttg
     2941 ccggtcgtcg aacagcgcag cagcagcctg atccgccggt agcctgacgc cacgatgagg
     3001 accctcgttt tgtctccaca tttcgatgac gccccgctca gtctcggcca gtcgatgctg
     3061 gatggccgcc tcgcgggtga gcgcgtcacc gtcggcgtcg tcttctcgca ctcgaactgg
     3121 acccggtggt ttcacccgac acggcggcgc tggccactcg ccaccgcgat ccgactcagc
     3181 gaggagtcgt tggcctcgat tcgtctgcgc taccggctcc gcctcggtgg attcgaggag
     3241 acggtgcttc gatccggaga tctgagcccg tccacctacc tggtccgcga tcctcagatc
     3301 ggcgacgacc cgatcgtcga cgcggtcgaa cgggtcgtgc gggattgggc ccagggggca
     3361 caacgcatca tcgccccgct cggggtcggc gaccaccgag atcatcagat cgtcaccgag
     3421 gcagcccgcc gattgctggt cgacgggctg cgggtggagt tctacgagga ccggccctat
     3481 gtgacctggg cggcgcacgg cgaggccgag cgaatcgcag cgctcgtcga cgaccgcctg
     3541 gagcgaacag ccgtgtcggg gccgatctcc gaggcgaagc accggcgcct cttctacccc
     3601 agccagttca ccgaggaatt caggggcgcg tttgccgccg acgaatctgg tgccgacggc
     3661 gagttcgtct gggcgcttcc gaacgcctga gtccgccgag atcagatgcg cttggactga
     3721 ccgccgtcta cggcgatgat ctggccggtg atccaactcg ccttgtcgct gaggagaaac
     3781 acgagtgcgt tcgccatgtc gaggggcgtt cccgggcgtt tgatcatcac gtcggtcaac
     3841 aagatgtcga agttctcctc gccaccggtg aggatctggg tcgcggcggt gttcgtcggg
     3901 ccgggggcga tggcgttgac gcggatgccg agcgatgcca tctccgccgc caggttgatg
     3961 gtgatcgagt tgagcgcggc cttggcgacc ccgtagaaac ccgaggccat ccacgccgcg
     4021 gtcgaggtct ggttgaccac tgcggcacct tcgttcttgc gcatctcgcg ccagcaggca
     4081 cgcgtcgcat acacggcgcc gttcatgttc acgtccatga acttgcggta gtagtcgagg
     4141 tcgatgcgca acaggccgtt gatctccatg ccgtggtaga tcgccgcatt gttgaccagg
     4201 tggtcgattc ggccgaactc ggccaccgcg gcctcggcca tggcgacgca ggactcgggg
     4261 tcggagacat cgaccttgac gccgagcgcc cggccgccgt cggcgacgat gtcggccgcg
     4321 accctgttgg cgttctcctc gttcagatcg gcgaccacca ccgccgcacc ctcgcgggcg
     4381 agcgcgtggg cgtactcctc gccgatgcct tggccggcgc cggtgacgat cgagaccttg
     4441 ccgtcgaact gtttggattc ggattgcatc gtggatgtcc cccgttggga tcaggaagcc
     4501 tgttgcttct tttcgaactg atggatggcc ttttcggcct gggtgttgat tcgcccgccg
     4561 cggggccacc cggcgtagtg gcacgcgagg atcacgatct cgcgcagttc ctcggtgctg
     4621 aggtcgccgc tcgacagcgc agcggggaac tggatatcca gcacatcttc ttggcccgaa
     4681 ccgatgagca ggccgatgag caacagacgc cgcgcccgat cgtcgaggcc cggacgattc
     4741 cagatctcac cgaagaggtg atcgacggtg taggagaaaa agtcgccggt gccgtcggtc
     4801 atctcccagc cgtacacctc ccccatgcgc tggaggttcg cgctgcgttc gggggatggt
     4861 tctacgtgat cggtcattgg tttcgtcctg tgttgagcgg tgaggaggcc acgccgagag
     4921 cgaaggccag ttggtcgagt ccgaggcggg cgatcggtac gtcgacgccg agttcgtcgg
     4981 cgagttcgat cgcctggctc aggtccttac ctccgagcga caagacgttc tccatcacgc
     5041 cgtaccacca gtcgtcgggg tcgatctggg cggtggtgtt tcgcacctgg atcgccccgg
     5101 ggccgccggt gacctggtcg gaatggcgca cgatccgacc gagttcctcg atgtcgatcc
     5161 cggcggcctc ggcgaggcgg ctcgcctcga gcgccgacgc gtagctcacg aagtgcatca
     5221 tgttgcgcgc caacttggtg cgggttcccg caccgacggg tccgagatgg ctgatcatgg
     5281 aacccatcag ttcgaggacc ggacgggcga cctcgagggc ctcgtcggtg ccgccgacca
     5341 tgatcgcgag gcggccgtcg ccggcgccca tcgccccacc cgagatcggc gcgtcgacga
     5401 cgtacacccc cttggcagcg gcaatttcgg cgaggtcttc ggcggtggtg gcccgaatcg
     5461 tcgagtggat gacgtaggcc tgtcccggcc gggcgacctc caacagtcca ccgacgacct
     5521 cgcgcacctg agcgtcgtcg cgcacgcaga tgcagatgac ctccgccgcc tcgccgagcg
     5581 ccgcgagcgg gtcggcgacg gtcgcaccgg cctccgcgaa cggagcggtg gcttcggccg
     5641 acaggtcgaa cacggcgagc ccgtcggggt tggacaacag ccgtttggcc atcggtgctc
     5701 cgatgttgcc aaggccgacg aatcctgcaa ccgccatcag atgccttccg cgatcgtctt
     5761 gagctcgagg tactcctcga acccggcgac ccccatctcg cggccgatgc cggactgctt
     5821 gtagccaccg aacggagcgt cggggctgta atagagccca ccgttcacgc tgatggtgcc
     5881 ggttcggatt cgccgtgcga ccgagcgggc acgttcgatg tcgttggagg tcaccgctcc
     5941 cgacagtcca taaaggctgt tgttggcgat ggccacggcg tcgtcgtcgc cgtcgtgggc
     6001 gatgaccgcc agcaccggtc cgaacacctc ctcctgggcg atgcgcgagt tctggtcgac
     6061 gttgacgagc acggtcggtt cgacgaagta tccgcccgcc aactccccct cgagcacggc
     6121 cggtttgccc gagccgagca ccagttcggc tccgtcgtcg accgcggtgt tgatgtagtc
     6181 gaggaccttg gttcgttgga cctcgctgac gatggggccc atgaagtttc cgggatcggt
     6241 cgggtcgccc cacccgattc cctgcatcat cgacacgagc gcatcgacga actcgtcctg
     6301 gcgggcacgc ggcaccaaca gccgggtcgt gatcgcacag ccctggccgg cgtgcgccat
     6361 gatctgaaat ccggcgccca tcgccacggc catcggatcg gcgtcgtcga gcgcgatgaa
     6421 cgccgacttg ccgccgagtt cgaggaacac ctttttgagg gtggccgagg cggccgccat
     6481 gaccttgcgt cccgtggccg tggagccggt gaagctgacc atgtcgacgc gggggtcggt
     6541 gcacaacacc tcgccgatgg cgttttcctg ggaggtgatg acgttgaaca cgcccgccgg
     6601 catgtcggtg tgttcggcca cgaggcgcgc caggttgttg gccgcccacg gggttgcggg
     6661 cgcagccttc aagacgacgg tgcagcccgc cgcgagcgcg gggatcacct tggccaggtt
     6721 gatctggtgc gggaagttcc atggcg
//
LOCUS       DAOOMA010000062         6723 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_13520_length_6723_cov_3.593881, whole genome shotgun sequence.
ACCESSION   DAOOMA010000062 DAOOMA010000000
VERSION     DAOOMA010000062.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 6723)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6723)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6723
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13520_length_6723_cov_3.593881"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..225
                     /locus_tag="PLP95_02925"
     CDS             <1..225
                     /locus_tag="PLP95_02925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB44783.1"
                     /translation="TDAPTTTAPVAAPGVSATEDIAVSEPSIGAPAPTQWVFPITRAF
                     PLGDSVVTFSWWGIGVFSADDFRLTGFAAF"
     gene            complement(351..1916)
                     /gene="serA"
                     /locus_tag="PLP95_02930"
     CDS             complement(351..1916)
                     /gene="serA"
                     /locus_tag="PLP95_02930"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004617 - phosphoglycerate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HPB44784.1"
                     /translation="MARVLVTEAIADNGLQRLRDAGHTVDIQLDWTPESLLELVKGAH
                     ALIIRSATQVTREVLEAGRDLVVVGRAGIGLDNVDTAAATELGVMVVNAPQSNVVSAA
                     EHTMAMLLSQARNIPQAHSALKAGRWERSKWNGVELGDKTLGIIGLGRIGRLVAQRAL
                     AFGMKLIAYDPFVSEDAARRMSTELVSLDELMARSDFVTLHLAKTPETINLINAELLA
                     KAKPDMRIINVARGGIINEADLADAVRNKVIAGAAIDVFDSEPCTESPLFDVDEIIVT
                     PHLGASTVEAQDKAGDTIADMVKLALSGEFVPFAVNVSAGEVSETVRPYQALAETLGG
                     LLAGLAGGAKGALEISYDGQIAEYDTRILTLSVLKGFFGAISEDPVSYVNAPQMAADK
                     GIDVNATQSTTPKDYVNLITVRIGSNSIAGTLAGLRAVSKIVMVDGFAVDIRPSANML
                     VVRNDDVPGMIGLIGTAVGASGVNISDMVVGEDADGVAALMVLSTSEAVADSVVADLE
                     AREGIVSVNRVRG"
     gene            complement(2032..4026)
                     /locus_tag="PLP95_02935"
     CDS             complement(2032..4026)
                     /locus_tag="PLP95_02935"
                     /inference="COORDINATES: protein motif:HMM:NF012546.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HPB44785.1"
                     /translation="MAELAPYGSWHSPITAQRVMSATARLGEVAITDDAVFWSEGRPA
                     EGGRIQIVKRTPDGELFDLLPDGFSASSSVHEYGGGAWWVDVDTLFFVNGADQRIYRC
                     DPGFVPGSKPTPVTPAPRRSRGLRYADASMTPDRRFIVCVQEAHPGEQGLPRATECVN
                     RLVAVPAVGGEPVVLREHADFVMSPRVDRLGEWVVWVEWSHPNMPWDATELWMGRLDT
                     SSGTPRLVNPTKVVGDGHESIVQPRWDHDNHLWFCSDRNDWWNLYHFNEQGAPTGEPH
                     VVAEGPWEVGTPPWVFGLSRYDFLSDDRVVFAYSSDGIDHLAVYDQLSTRVDRLEVPF
                     TSVQQVQAWTTTVVFVGGSFTLSASVVATVVGRNGATSRIENMRPVADPPMTSAYISV
                     GQPVTYPTVHGVAHGIYYPPTNPDFVAPEGTRPPLVVMIHGGPTASATPELSLRTQYW
                     TSRGFAVVDVNHRGSTGFGRQFRDLLRGEWGVVDVEDCIAAGEFLIGAGRADPDRLII
                     RGGSAGGFTTLAALTFHDAFSAGCSLYGIADLELLVGDDHKFESRYTFSLVANLPEGR
                     EVFIERSPIHHIERLTAPVILFQGTEDLVVPANQAEVMAAALDARGVPHSAVFFAGEG
                     HGFRQADNIVRCLEAELSFYAQTLGFPHPEGVDPVEVKHL"
     gene            complement(4179..5402)
                     /locus_tag="PLP95_02940"
     CDS             complement(4179..5402)
                     /locus_tag="PLP95_02940"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012230296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HPB44786.1"
                     /translation="MTESSASHKPRISRRIAAIAPSATLAVDAKAKALKAKGEPVIGF
                     GAGEPDFATPDYIVAAAIEAAKNPVAHRYTPAGGLPALQAAIAAATLRDSGVEVEPSQ
                     VIVTNGGKHALYNAFATLLDPGDEVLLPAPYWTTYPESIALAGGVTVELPTDESTGFR
                     ATVEQLEAARTERTKALVFVSPSNPTGAVYPRDEVEAIGRWAVEHGIWVITDEIYQHL
                     VYGDNEFTSMPGVVPELRDTCLIVNGVAKTYAMTGWRVGWIVGPTDVIKAATNLQSHA
                     TSNVSNVAQYAAIAALEGGLDAVDEMRAAFDRRRVTMWKMLSEIPGVTCLEPEGAFYA
                     FPNFTGLLNISLNGRVATTTLELADLILEEVNVAFVPGEAFGAPGYARFSFALSDEDM
                     IEGIRRIADLVASSS"
     gene            5514..6632
                     /gene="serC"
                     /locus_tag="PLP95_02945"
     CDS             5514..6632
                     /gene="serC"
                     /locus_tag="PLP95_02945"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004648 -
                     O-phospho-L-serine:2-oxoglutarate aminotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine transaminase"
                     /protein_id="HPB44787.1"
                     /translation="MSDTINIPSDLLPADGRFGCGPSKIRPEAVEALAASTGYLGTSH
                     RQAAVLFEVGALRNAVAELFSLPDGYEVLIGNGGSTLFWDAATFGLIRERSQHLVFGE
                     FSSKFFSASKAAPFLGDPSKVESEVGTHPVSVAEDGIDLYAMTHNETSTGVAMPLTRP
                     AGIAADALVAVDATSGAGGLRVDMNEVDVYYFAPQRCLASDGGLWIAACSPRAIERIE
                     QIRASGRWTPAMLDLGIALDNSRKDQTYNTPALATVFLANQQIRWVLDNGGLEFAAGR
                     CDASAQTLYGWADTHELATPFVTDAAMRSHVVATIDFAGTVDAKRLAAVLRANGIVDV
                     EPYRKLGRNQLRVAMFPAVDPEDVSALTACIDWVVAQG"
BASE COUNT         1113 a         2325 c         2211 g         1074 t
ORIGIN      
        1 accgacgccc ccaccaccac ggcacccgtt gccgcaccgg gtgtttcggc aacggaggac
       61 atcgctgtct ccgaaccgtc gatcggcgcc ccggccccga cgcaatgggt gttcccgatc
      121 acccgggcgt tccccctcgg cgattcggtc gtcacgttct cgtggtgggg catcggggtg
      181 ttcagcgccg acgacttccg cctcaccggg ttcgcagcgt tctagcccgc accaccagcc
      241 cagctcgcgt cagcgttctg taggtaatcg cggtggccat aacaaccgcg attacctaca
      301 gaacggccaa cggcgcccaa cggcggccaa cggcggccaa cggcggcgcg tcagccgcga
      361 acgcggttga cgctcacgat tccctcgcga gcctcgagat cggcgaccac cgaatccgca
      421 accgcctcgg aggtcgacag caccatgagt gccgccaccc catcggcgtc ctcgccgacg
      481 accatgtcgg agatgttcac ccccgaagcc cccacggcgg tgccgatgag gccgatcatg
      541 cccggcacgt cgtcgttgcg cacgacgagc atgtttgccg acggacgaat gtcgaccgcg
      601 aacccgtcga ccatcacgat cttcgaaacg gcgcgcagcc ccgcaagcgt cccggcgatg
      661 gagttcgagc cgatccgcac cgtgatcagg ttcacgtagt ccttcggggt cgtcgactgc
      721 gtggcgttca cgtcgatgcc cttgtcggcg gccatctgcg gagcgttcac ataagacacc
      781 ggatcttcgc tgatggcgcc gaagaacccc ttgagcaccg acagggtgag gattctcgtg
      841 tcgtactcgg cgatctgtcc gtcgtagctg atctcgagcg cccccttcgc gcccccggcg
      901 aggccggcga gcaggccgcc gagcgtctcg gccaaggcct ggtacggccg gaccgtctcg
      961 gacacctcac ccgccgagac gttgaccgcg aacggcacga attcgccgga cagggcgagc
     1021 ttgaccatgt cggcgatcgt gtcgcccgcc ttgtcctgcg cttcgaccgt ggaggccccg
     1081 aggtgcgggg tcacgatgat ctcgtcgacg tcgaacaacg gggattcggt gcacggctcg
     1141 gaatcgaaca cgtcgatggc tgcgccggcg atcaccttgt tgcgcaccgc gtcggcgagg
     1201 tcggcctcgt tgatgatgcc accgcgggcg acgttgatga tgcgcatgtc gggtttggct
     1261 ttggcgagca actcggcgtt gatcaggttg atcgtctcgg gggtcttcgc caggtgcaac
     1321 gtgacgaagt cggatcgggc catcagctca tccaggctca ccagctcggt ggacatgcgc
     1381 cgggctgcgt cctcgctgac gaacgggtca tacgcgatga gcttcatgcc gaacgcgagc
     1441 gcacgctgag ccaccaaccg cccgatccgg ccgagaccga tgatgccgag ggtcttgtcg
     1501 cccagttcga cgccgttcca cttcgaacgc tcccagcgcc ccgccttgag cgccgaatgg
     1561 gcctggggaa tgttgcgggc ctggctcagc aacatggcca tggtgtgttc cgccgccgac
     1621 acgacgttgg actgcggggc gttcaccacc atcacgccga gttcggttgc cgcggcggtg
     1681 tcgacgttgt cgagcccgat gcccgcacgc ccgaccacga ccaggtcgcg tcccgcctcg
     1741 agcacttcgc gggtcacctg cgtcgccgac cggatgatca gcgcgtgcgc ccccttcacc
     1801 agttcgagga gcgattcggg ggtccagtcg agctggatgt cgaccgtgtg cccggcgtcg
     1861 cgcagacgct gcagaccgtt gtcggcgatt gcttcagtta cgagtactcg tgccacgtcc
     1921 gccgatgcta ccggcgcggc gccccggccc cgaaccgaga ttcaccaccg tgacgggtgt
     1981 cgcaacgttg cgaccgacga cacgtcagcg cccatggcgc gaggcgagcg ttcacaggtg
     2041 cttgacctcg accgggtcga ccccctcggg atgtggaaac ccgagggtct gcgcgtagaa
     2101 gctcagctcg gcctccaggc accgcacgat gttgtcggcc tggcgaaagc cgtggccttc
     2161 ccccgcgaag aacacggcgc tgtgcggcac gccgcgggca tcgagcgcgg cggccatcac
     2221 ctcggcctga ttcgccggaa ccacgaggtc ctcggtgccc tggaacaaga tgaccggagc
     2281 ggtgaggcgt tcgatgtgat ggatgggcga gcgctcgatg aacacctcgc gcccctcggg
     2341 caggttggcg accaggctga aggtgtagcg cgattcgaac ttgtggtcgt cgcccaccaa
     2401 cagctcgaga tccgcgattc catacagcga acaaccggcg ctgaacgcat cgtggaacgt
     2461 gagcgccgcc aaggtcgtga atccaccggc ggacccgccg cggatgatca accgatccgg
     2521 atcggcacgg cctgcgccga tcaggaactc gccggcggcg atgcaatcct cgacgtcgac
     2581 gacaccccac tcgccgcgca acaggtcgcg gaactggcgg ccgaacccgg tgctgccacg
     2641 gtgattcaca tcgaccacgg cgaacccccg ggaggtccag tactgggtcc gaaggctgag
     2701 ttccggggtg gctgatgcgg tcggaccgcc gtggatcatg accaccagcg gcggacgggt
     2761 cccctccggt gcgacgaaat cggggttcgt cggcgggtag tagatcccgt gggcgacacc
     2821 gtgaacggtc ggatacgtga cgggttggcc gaccgagatg tacgccgagg tcatcggcgg
     2881 gtcggccacc ggacgcatgt tctcgatgcg cgaggtcgca ccgtttctgc cgacgacggt
     2941 cgccacgacc gacgcggaca gcgtgaaact cccgccgacg aacacgacgg tggtggtcca
     3001 ggcctgaacc tgttgcaccg acgtgaaggg cacctcgagt cgatcgacgc gggtgctgag
     3061 ctggtcgtac accgcgagat gatcgatgcc gtcgctggaa taggcgaaca cgacccggtc
     3121 atcggaaaga aagtcgtagc gcgacagacc gaacacccac ggcggcgtgc cgacctccca
     3181 ggggccttcg gcaaccacgt gcggttcgcc ggtcggcgca ccctgctcgt tgaagtggta
     3241 caggttccac cagtcgttgc gatccgaaca gaaccacagg tgattgtcgt gatcccaccg
     3301 cggttgcacg atcgactcgt ggccgtcgcc gaccaccttg gtcgggttca cgagccgcgg
     3361 ggttccactc gacgtgtcca ggcggcccat ccacaactcg gtggcgtccc agggcatgtt
     3421 cgggtgcgac cattcgaccc acaccaccca ctcgcccaac cggtcgaccc gcggcgacat
     3481 gacgaaatcg gcgtgctcgc gcaacaccac cggctcgccg cccaccgccg gcacggcgac
     3541 cagccggttg acacattcgg tggctcgcgg caggccctgt tcgcccggat gggcctcctg
     3601 aacacagacg atgaagcgac gatcgggggt catggacgcg tcggcgtacc gcaggcctcg
     3661 cgaccgtcgc ggcgccgggg tgaccggcgt cggcttggat ccggggacga acccgggatc
     3721 gcaccggtag atccgttgat cggcgccgtt gacgaaaaac agcgtgtcga cgtcaaccca
     3781 ccacgcaccg ccgccgtact cgtgcaccga actcgacgcc gagaatccgt cggggaggag
     3841 gtcgaacagc tccccgtcgg gggtgcgctt cacgatctga atccggccgc cctcggccgg
     3901 acgcccctcc gaccagaaca cggcgtcatc ggtgatcgcc acctcgccga ggcgagcggt
     3961 ggccgacatc acgcgctgcg cagtgatcgg tgagtgccag gagccgtagg gcgcgagttc
     4021 agccatgccg tgtcgacgat atcgcgggcg cttgccgaaa ccgcctgatg ccctcgaaac
     4081 ggccccacgc ccccccgacg cgaacacgac cccacgcgaa cacgccccga cgcaagcccc
     4141 agggccgtcg gggcgtgttc acgagctggc gagaaaggtc aggagctgga cgcgacgaga
     4201 tccgcgatgc gacggatgcc ctcgatcatg tcctcgtcgg agagcgcgaa gctgaaccgg
     4261 gcgtatcccg gcgcaccgaa ggcttcgccc ggaacgaagg ccacgttgac ctcctccaag
     4321 atgaggtcgg cgagttcgag cgtcgtggtg gcgacccgac cgttgagcga gatgttgagc
     4381 aggcccgtga agttcgggaa ggcgtagaag gccccctccg gttcgaggca ggtgaccccg
     4441 ggaatctccg agagcatctt ccacatcgtc acccgtcggc ggtcgaaggc cgcacgcatc
     4501 tcgtcgaccg cgtcgaggcc gccctcgagc gccgcgatcg ccgcgtactg ggccacgttg
     4561 ctgacgttgg aggtcgcgtg gctctgcagg ttcgtcgcgg ccttgatcac gtcggtcggc
     4621 ccgacgatcc aaccgacccg ccagccggtc atcgcgtagg tcttggcgac cccgttgacg
     4681 atcaggcagg tgtcgcgcag ttccggcacg actcccggca tcgaggtgaa ctcgttgtcg
     4741 ccgtagacca ggtgctggta gatctcgtcg gtgatcaccc agatgccgtg ttcgacggcc
     4801 cagcggccga tggcctccac ctcgtcgcgg ggatacaccg caccggtcgg gttggagggc
     4861 gacacgaaca ccagcgcctt ggtccgttcg gttcgcgcgg cctcgagttg ttcgaccgtc
     4921 gcccggaatc cggtcgactc gtcggtgggc aactcgacgg tgaccccgcc ggccagagct
     4981 atcgactcgg gatacgtcgt ccagtacggg gccggcaaca agacctcgtc gccggggtcg
     5041 agcagggttg cgaacgcgtt gtacagggcg tgtttgccgc cgttggtgac gatgacctgc
     5101 gacggttcca cctcgacccc ggagtcgcgc agcgtcgccg cggcgatcgc ggcctgcagc
     5161 gccggcagcc caccggccgg ggtgtagcgg tgtgccaccg ggttcttcgc cgcctcgatc
     5221 gcggcggcga cgatgtagtc gggggttgca aagtcgggtt caccggcacc gaaaccgatg
     5281 accggctccc ccttcgcctt cagcgccttg gccttcgcgt cgaccgcgag ggttgcggac
     5341 ggggcgatcg cggcgatgcg ccgggaaatt cgtggcttgt gggatgcgct tgattcggtc
     5401 acgtgggcaa ttcttggccg cacgacggcc cgcgtcgaat tcatttccca cccacctcag
     5461 ccatctgtcg aactgatttc caccgagggg cgatcattgg gagactgagg gcgatgagcg
     5521 acacgatcaa catcccctcc gacctgctcc ccgcggacgg ccgcttcggt tgtggccctt
     5581 ccaagatccg ccccgaggcg gtcgaggccc tggcggcctc gaccggctat ctgggcacgt
     5641 cgcaccgcca ggctgcggtg ctcttcgagg tcggagcgct gcgcaacgcc gtcgccgaac
     5701 tgttctccct gcccgacgga tacgaggtcc tcatcggaaa cggcgggtcg accctgttct
     5761 gggacgccgc caccttcggg ctcatccgcg agcgcagcca gcacctggtg ttcggagagt
     5821 tcagctcgaa gttcttctcc gcgtcgaaag cggcgccctt cctcggtgac ccgtcgaagg
     5881 tcgaatccga agtcggcacc cacccggtgt cggtggccga ggacgggatc gatctctacg
     5941 cgatgaccca caacgaaacc tcgaccgggg tcgcgatgcc gctgacccgc cccgccggca
     6001 tcgccgccga tgcgctcgtc gccgtcgacg ccacctcagg cgcgggcggg cttcgcgtcg
     6061 acatgaacga ggtcgacgtc tactacttcg ccccccaaag gtgcctcgcc tccgacggcg
     6121 ggctgtggat cgcagcgtgt tcgccacgcg ccatcgaacg catcgaacaa atccgtgcct
     6181 ccgggcgttg gaccccggcc atgttggacc tgggcattgc gctcgacaac agccgcaagg
     6241 accagaccta caacacgccg gccctcgcca cggtgttcct ggccaaccag cagatccgct
     6301 gggttctcga caacggcggg ctcgagttcg ccgccgggcg ctgtgacgcg tcggcacaga
     6361 cgctgtatgg ctgggccgat acccacgagt tggcgacccc cttcgtcacc gatgccgcca
     6421 tgcggtccca tgtggtcgcc acgatcgact tcgccggcac cgtcgacgcc aaacgcctcg
     6481 cggcggttct gcgggcaaac ggcatcgtgg atgtcgagcc ctaccgcaag ctcggccgca
     6541 accagctgcg ggtcgcgatg ttccccgccg tcgaccccga ggacgtgagc gccctcaccg
     6601 cgtgcatcga ctgggtcgtc gcccagggct gagcgcagag gcgcacgcgg gagcgcgcgt
     6661 cagcgagcga cctcgggctc ggacgagccg acgaggtctg cgtcggccgg tccgacggtg
     6721 agc
//
LOCUS       DAOOMA010000063         6635 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_13769_length_6635_cov_3.814590, whole genome shotgun sequence.
ACCESSION   DAOOMA010000063 DAOOMA010000000
VERSION     DAOOMA010000063.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 6635)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6635)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6635
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13769_length_6635_cov_3.814590"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..202
                     /locus_tag="PLP95_02950"
     CDS             <1..202
                     /locus_tag="PLP95_02950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB44788.1"
                     /translation="VFSFAVKANNRNLRVIERDLADREATRRESWKVLIAVVAMFVLT
                     VAVFVAGIAVFTWVVVDWLFGG"
     gene            337..1038
                     /locus_tag="PLP95_02955"
     CDS             337..1038
                     /locus_tag="PLP95_02955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB44789.1"
                     /translation="MNSTTTQPNPTTPRSTGQWLVDQRNAGATPEEIADLLVRAGWSA
                     DAAADTALRSLRSVDRHRLLYHGLCWGAGLGSLALGSALHLAIANPQHLDDRTTMALA
                     ITVAIVLLPIAATCGYFVHRVEHEDRFAVFSPTRRTLFGWLAGCTAVVGISRLITYLY
                     QLVSSLVGASGEDLGIDAFGQVAVTLVIAVPLFAWSIVQWRRSNVALRSLGRNVGDGP
                     GLGNGHEYLGGGDQR"
     gene            1025..2161
                     /locus_tag="PLP95_02960"
     CDS             1025..2161
                     /locus_tag="PLP95_02960"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPB44790.1"
                     /translation="MINGERADPRLRELLVGTGALRVLTLVWSTVVTIIDARSGVLER
                     TGVAFGGLLVMAAWTAWWFRTLAVSPTKLTNAPSMAIDLGVAMAVTAADAWVYAGDHP
                     QSYGSAWPIAAVLSIGATAGMSTGALAGLALGVTRIVAAAIGDATGGAWLALTGSVLL
                     AVIAGVVAGYVSGRLRRAESEVALARAREEFARDLHDGVLQTLAVIQRRSDDDQLVAL
                     AREQDRELRAFIGDTTTTTPLPTNLVADLQRAAARVSGRELIEVNVVVIDAPDGPDPR
                     HEALVAAVGEALTNAAKHGRATVVTVCVDADEDTLTVTINDDGVGFDPVTTTEGVGLT
                     RSIRGRLNDVGGTATVRSRPGHGTEVELRVPTSAAPRSAREETR"
     gene            2158..2796
                     /locus_tag="PLP95_02965"
     CDS             2158..2796
                     /locus_tag="PLP95_02965"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HPB44791.1"
                     /translation="MTDRLTRSLRIVIADDHEIWLSGLRADLGQYFTVVAAANDATEA
                     IEAIRRERPDLALVDLHMGGGGGLAVVRACAAEVPIVILTVSEAERDLLDAVAAGAVG
                     FLTKSTRSEELRIALHRAVDGEPVFSPQLAALVLGEFRRLATSSPPEQALSPREREIV
                     GLVARGHPYRTIAEELFISPKTVENHVRNIMAKLHLSRRHELIRWAVDRGIE"
     assembly_gap    2861..2960
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(3004..3945)
                     /locus_tag="PLP95_02970"
     CDS             complement(3004..3945)
                     /locus_tag="PLP95_02970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB44792.1"
                     /translation="MGVGPLQMSLPAGLATVIGALPQLSARDAAAMVTQFVPDLPAAP
                     TVVLEAEAAELLAGTAGYVSDRDADSLAGARAFLESLRGRVDPVVMSLTGPVTVGLRL
                     LGAGVSGDEASRLARDYVEEWAGVILDMAAQVLPDAPVMLFFEEPGLSNSMHPTFPMS
                     PAAIDRLVGGVVEAIAERAFVGIQVSGRADWGLLVRTGIRVLGAPISARLDSAAADLQ
                     RFFDEGGFIAWGAVPVDEPLGPNSDRLWRRLSEIWAELAMAGLDPLQLRERSIITPAA
                     NLHAFGASQTERVLRLTNELSQRVFHQVTGARFAVGA"
     gene            3992..4891
                     /locus_tag="PLP95_02975"
     CDS             3992..4891
                     /locus_tag="PLP95_02975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB44793.1"
                     /translation="MRSHPIVWLLRVAWLVLPLTLGTVIEDALDGRSTPVVVVTAVLS
                     WTLWALGLGAALVTVPAAHAVLRWVVPIALVAGITSMPMNRPDTGERAVANALGVIGL
                     IAVLVAAACAFAGVLANEYANGAAYGDERRFALRIPAGFLLGPIPVFWAATAGGLVGG
                     PVLLAAQAWLPGTLTLIVGLVAAPVAARSFLGLSRRWLVFVPAGVTLVDHLALSDPVL
                     FSKARITRFGPAFTDTVAKDLTSNAAGLVIELAFDQPLDISVRVGRDQAEVELVRAVL
                     ITPVRPGAVMQEAARRGLPTSAQ"
     gene            complement(4927..6009)
                     /gene="mnmA"
                     /locus_tag="PLP95_02980"
     CDS             complement(4927..6009)
                     /gene="mnmA"
                     /locus_tag="PLP95_02980"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020378341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HPB44794.1"
                     /translation="MSASNPSRVLVAMSGGVDSSVAAALLQRAGHEVVGVTMKLWGGQ
                     SDSGCCSVSDVDDARRVAQQLGIDHHVFNFGDEFDEFVVEPYVADHVAGRTPNPCVEC
                     NRHIKFDKLLRRADALGFDLVATGHHARRVQRGDHFRIARGADDRKDQSYVLYPLVGS
                     ALARTIFPLGEMTKPQVRALAEELGLRTAAKPDSQDVCFIAKSTGGRSVFLGERIPMR
                     SATVVDTSGQRVGELDSIELVTIGQRRGLDVGGPGGRRYVVDVDTAAQVVTVGGLEDL
                     LSDRVELAEVVYADASSAGDALVGRAVGVQCSAHGREAPAVVAASGRAASLTIGWNEP
                     QRRVAPGQSVVLYDGDEVIAGGVVAR"
     gene            complement(6078..>6635)
                     /locus_tag="PLP95_02985"
     CDS             complement(6078..>6635)
                     /locus_tag="PLP95_02985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HPB44795.1"
                     /translation="LVAQHIGGGQEREWRSGTHNVAGIAAMAAAAAEADAQRDAEVER
                     LRALRDRLVERVVAGLGAAGGQAAGDGAGEAAGDGAGVSWTGARIEPAHLAAGFAHFC
                     FDGVESEALLFLLDDAGVAASAASACASGAQHPSHVLEAMGIGRDSARGSLRLSLGHT
                     TTDSEVDLAADAVIDSVRRLRSHRH"
BASE COUNT          996 a         2373 c         2171 g          995 t
ORIGIN      
        1 ggtgttctcc ttcgccgtca aggccaacaa ccgcaacctg cgggtgatcg aacgcgacct
       61 cgccgaccgc gaggcgaccc gccgggagag ctggaaggtc ctcatcgcgg tcgtggcgat
      121 gttcgtgttg accgtggccg tgtttgtggc gggcatcgcg gtgttcacct gggtggtcgt
      181 cgactggctc ttcggcggtt agccgtccga cggcgcaggt cgtcccagcg cggcacatcg
      241 gggagccccc aggcggatct gggggatcgc cccgatggtg gtgtgcccgt tcgaggtcga
      301 tggtgatcgg gagaactcac ccacggagga cccacgatga actcgaccac cacccagccg
      361 aacccgacga cccccaggag caccggacag tggctggtcg accagcgcaa cgccggggcc
      421 acccccgagg agatcgccga tctgctcgtg cgcgccggtt ggtcggccga cgccgccgcc
      481 gacacggcgc tgaggtcgct gcgcagcgtc gatcgccacc gcctgctgta ccacgggctg
      541 tgttggggcg ccggcctggg ttcgctcgcc ctcggcagcg ccttgcacct ggccatcgcc
      601 aacccacagc acctcgacga tcggaccacc atggcgcttg ccatcacggt ggccatcgtc
      661 ctgctcccga tcgccgccac ctgcggctac ttcgtgcacc gggtcgaaca cgaggaccgc
      721 ttcgccgtgt tctcccccac tcggcgcacc ctcttcggct ggctcgccgg ctgcaccgcg
      781 gtcgtcggga tctcgcggct catcacctac ctgtaccaac tcgtttcctc cctggtgggg
      841 gcatcgggtg aggacctggg aatcgatgcg ttcggccagg tggcggtcac cctcgtcatc
      901 gccgtgccgc tgttcgcctg gtcgatcgtg cagtggcgcc gctcgaacgt cgcgttgcgt
      961 tccctcggcc gcaacgtcgg cgacgggccc ggcctgggca acggccacga gtaccttggc
     1021 ggaggtgatc aacggtgagc gagcggaccc acgactacga gaactgctcg tcggaacggg
     1081 cgcgctgcgg gtcctcaccc tcgtgtggag caccgtggtc accatcatcg acgcccgctc
     1141 cggcgtgctc gaacgaaccg gggtggcgtt cggcgggctc ctggttatgg ccgcgtggac
     1201 ggcgtggtgg ttccgcaccc tcgcggtctc gccgacgaag ctcaccaacg ccccgtcgat
     1261 ggcgatcgac cttggggtcg ccatggcggt caccgccgca gacgcctggg tgtatgcggg
     1321 cgaccacccc cagagctacg gatcggcctg gccgatcgcg gccgtgttga gcatcggtgc
     1381 gacggcgggc atgtccaccg gagccctcgc cgggttggcc ctgggcgtga cccgaatcgt
     1441 ggcggcggcg atcggcgacg ccaccggggg tgcctggttg gcgctcaccg ggtccgtgct
     1501 cctggccgtc atcgccggtg tcgtcgcggg ctacgtcagc ggtcggctcc ggcgcgccga
     1561 atcggaggtc gcgctcgccc gggcgcgcga ggaattcgcc cgcgacctgc acgatggcgt
     1621 gctgcagacc ctggccgtca ttcagcgacg aagcgacgac gaccaactcg tcgcgttggc
     1681 ccgggaacag gaccgcgaac tgcgtgcatt catcggcgac accacgacca cgacaccgtt
     1741 gccgaccaat ctcgtcgcag atctgcaacg cgccgcggct cgtgtttccg ggcgcgaact
     1801 catcgaagtg aatgtcgtgg tgatcgatgc ccccgacgga cccgacccac gtcacgaggc
     1861 actcgtcgct gcggtcggcg aggcgctcac caacgctgcc aaacacggcc gcgcaacggt
     1921 cgtgaccgtg tgtgtcgacg ccgacgagga cacgctgacc gtgacgatca acgatgacgg
     1981 ggtcgggttc gaccccgtca ccaccaccga aggggtcgga ctgacgcgtt cgattcgcgg
     2041 ccgactcaac gacgtcgggg gcaccgcgac ggtgcggtcc cggcccggac acggcaccga
     2101 ggtcgagctg cgcgttccga catcggcggc gccgcgctcc gcccgagagg agaccagatg
     2161 accgaccggt tgacccgatc gcttcgcatc gtcatcgccg acgaccacga gatctggttg
     2221 tcgggcctgc gagccgacct cgggcagtac ttcaccgtgg tcgccgcggc caacgacgcc
     2281 accgaggcca tcgaggccat ccgacgagaa cgacccgacc tcgcgctcgt cgacctgcac
     2341 atgggcggtg ggggcggcct cgcggtcgtg cgggcctgcg ccgcggaggt ccccatcgtc
     2401 atcctcaccg tgtcggaggc cgagcgcgac ctgctcgacg ccgtcgctgc cggagcggtc
     2461 gggttcctca ccaagtcgac acggtccgag gagctgcgca tcgcactgca tcgcgccgtc
     2521 gacggcgagc cggtgttctc gccccagctc gccgcactgg tcctcggaga gttccgacgc
     2581 cttgcaacgt catcgccgcc cgagcaggcg ctgtcgccgc gcgaacgcga gatcgtcggg
     2641 ctggtggcac gggggcaccc ctaccgcacc atcgcggagg agcttttcat ctctcccaag
     2701 accgtcgaga accacgtgcg caacatcatg gcgaagctgc acctgtcccg acgccacgaa
     2761 ctcatccgct gggcggtcga ccgcgggatc gaatagcacg acccttacgc gcctacggta
     2821 cgaccctcac gcgtctacgg cgcagtgctc acgagactac nnnnnnnnnn nnnnnnnnnn
     2881 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2941 nnnnnnnnnn nnnnnnnnnn catgcgccta cggcacagta ttcatgcgcc tacggcacag
     3001 tattcatgcg cctacggcaa aacgcgcccc ggtgacctga tggaagaccc gctgggacag
     3061 ctcgttggtg aggcgcagca cccgttcggt ctggctggcg ccgaacgcgt gcaggttcgc
     3121 cgccggggtg atgatcgaac gttcgcgaag ctggagcggg tcgagccccg ccattgccag
     3181 ctcggcccag atctccgaca gccgccgcca cagacgatct gagttcggcc ccaacggctc
     3241 atcgaccggc accgcacccc atgcgatgaa cccgccctcg tcgaagaacc gctgcagatc
     3301 ggcggcggcc gagtcgaggc gtgcactgat cggtgcgccg agcacccgga tgccggtccg
     3361 tacgagcagc ccccagtcgg cgcggccact gacctgaatg ccgacgaacg cccgctcggc
     3421 gatcgcctcc acgacgccgc cgacgagacg gtcgatcgcc gcgggcgaca tcggaaacgt
     3481 cgggtgcatc gagttcgaca ggcccggctc ttcgaagaac aacatgaccg gagcgtcggg
     3541 caggacctgc gccgccatgt cgaggatcac cccggcccat tcttcgacgt agtcgcgagc
     3601 cagccgactg gcctcgtccc cactcacccc cgccccgagg agtcgcaggc caaccgtcac
     3661 cggaccggtg agcgacatga ccaccgggtc gacccgtccg cgcaggctct ccagaaatgc
     3721 ccgtgcaccc gccagcgaat cggcatcgcg atcgctgacg taaccggcgg taccggcaag
     3781 cagttccgcg gcttcagctt cgaggacaac ggtgggtgct gcaggaagat cgggaacgaa
     3841 ctgcgtcacc atcgccgccg cgtcgcgggc gctcagctgc ggcagtgcgc cgatgaccgt
     3901 ggccaatccg gcgggtagcg acatctgcag cggaccaacc cccatccgtc ctgcttagcg
     3961 atgccgccgg attctttgca agtctgtggc catgcgatcc caccccatcg tctggctcct
     4021 gcgcgtcgcg tggctggttc tccccctcac cctcggcacc gtgatcgaag acgcactcga
     4081 tggccgctcc acccccgtcg tcgtggtcac cgcagtgctg tcgtggacgc tgtgggccct
     4141 cggtttggga gcggcgctgg tcaccgtgcc cgcagcgcat gcggtcctgc gctgggtcgt
     4201 ccccatcgcc ctggtggcgg gcatcacatc gatgccgatg aaccgtcccg acacggggga
     4261 acgggccgtc gcgaacgccc tcggggtgat cggcctgatc gccgtcctcg tcgccgctgc
     4321 gtgcgcgttc gccggtgtcc tggccaacga gtacgcaaac ggcgctgcct acggcgacga
     4381 acggcgcttc gcccttcgca tccccgcagg attcctcctc gggcccattc ccgtgttctg
     4441 ggcggcgacc gccggagggc tcgtcggcgg tccggtcctg ttggcggcgc aggcgtggct
     4501 ccccggaacg ttgacgttga tcgtcggcct cgtggccgcc cccgtggcag ctcgaagctt
     4561 cctcgggctg tctcggcgat ggctggtgtt cgtgccggcg ggggtcaccc tggtcgacca
     4621 cctcgcgctg agcgaccccg tgttgttctc caaagcccgc atcacccggt tcggaccggc
     4681 cttcaccgac accgtggcca aggacctcac cagcaacgct gcgggcctag tgatcgagct
     4741 tgcattcgac cagccgctcg acatctccgt gcgcgtcggg cgagatcagg ccgaggtcga
     4801 actcgtgcgg gcggtgttga tcaccccggt gcgcccgggc gccgtgatgc aagaggccgc
     4861 acgccggggt ctgcccacct ccgcgcagtg atgacgactc agtgatgacg acgcagaaat
     4921 gacggctcag cgggcgacga cgccgccggc gatcacctcg tcgccgtcgt agagcaccac
     4981 gctctgcccg ggagcgacgc gtcgctgcgg ctcgttccag ccgatggtca gcgacgccgc
     5041 ccgccccgat gcggcgacca cggcgggcgc ctcacggccg tgcgccgagc actggacccc
     5101 aaccgcccgg ccgacgagcg cgtcgccggc tgacgacgca tcggcgtaca cgacctccgc
     5161 gagttcgacc cgatcgctca acaggtcctc aagcccgccg acggtcacga cctgggccgc
     5221 ggtgtcgacg tcgacgacgt agcgccgacc cccgggtccg ccgacgtcga gcccccgccg
     5281 ttgaccgatg gtcacgagtt cgatgctgtc gagttccccc acgcgctgac cggaggtgtc
     5341 cacgaccgtg gccgatcgca tcggaatccg ctcgccgaga aacacgctgc gcccgccggt
     5401 cgacttggcg atgaaacaca cgtcctggct gtcgggcttg gccgcggtgc gcaggcccag
     5461 ttcctcggcg agcgcccgaa cctgaggctt ggtcatctcg ccgaggggaa agatcgtgcg
     5521 ggcgagcgcc gagccgacca gcgggtacag cacatagctt tgatccttgc gatcgtcggc
     5581 accgcgggcg atgcgaaagt ggtcgccccg ctggacccgg cgggcgtgat gcccggtggc
     5641 cacgaggtcg aaccccaggg catcggcgcg acgcaacagc ttgtcgaact tgatgtgacg
     5701 gttgcactcc acacacggat tgggagtgcg gccggcaacg tggtcggcca cgtacggttc
     5761 gacgacgaac tcgtcgaact cgtcgccgaa attgaagacg tggtggtcga ttccgagctg
     5821 ctgggcgacc cgtcgtgcgt cgtcgacatc ggagaccgaa cagcaccccg agtcgctctg
     5881 cccgccccag agcttcatcg tgaccccgac gacctcgtgg ccggcgcgct gcaacagcgc
     5941 tgcggcgacc gaactgtcga cgccgccgga catggcaacc aggactcgcg agggattcga
     6001 agcgctcacc gccccattgt cgcgcctcga ggctcgccgg ccgcgatcgg ccgccgatgg
     6061 gatcgatgga gatgggatca atgacgatgc gaacggagtc ggcgcaccga gtcgatcacc
     6121 gcgtccgcgg cgaggtcgac ctcggagtcg gtcgtggtgt gaccgaggga caacctcagc
     6181 gagccccgtg ccgagtcgcg tccgatcccc atcgcctcga gcacatggga gggatgctgc
     6241 gcgcccgacg cgcacgcgga ggcggccgag gcggccacac cggcgtcgtc gagcaggaac
     6301 agcaacgcct cggactcgac gccgtcgaaa cagaagtggg cgaaccccgc cgcgaggtga
     6361 gcgggctcga tgcgagcccc ggtccacgac accccggcgc catccccggc cgcttccccc
     6421 gcgccatccc ccgccgcttg cccgccggcc gcaccgagcc ctgccacgac gcgttcgacc
     6481 agtcggtcgc gcagcgccct cagacgttcg acctcggcgt cgcgctgcgc gtcggcctct
     6541 gcggcggcgg ccgccatcgc cgcgatcccc gccacgttgt gggtgccgct gcgccactct
     6601 cgctcctggc ctcccccgat gtgttgggcc acgag
//
LOCUS       DAOOMA010000064         6617 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_13819_length_6617_cov_4.213959, whole genome shotgun sequence.
ACCESSION   DAOOMA010000064 DAOOMA010000000
VERSION     DAOOMA010000064.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 6617)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6617)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6617
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13819_length_6617_cov_4.213959"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1323
                     /gene="rho"
                     /locus_tag="PLP95_02990"
     CDS             <1..1323
                     /gene="rho"
                     /locus_tag="PLP95_02990"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF006886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HPB44796.1"
                     /translation="PAPTVAADDATGPASGPAAGDTAGDGQPGEPLGSDTADVSSATN
                     GDSAAAAAREATDGTTGAAGDEEAADEGSTDGSTDGSEDAAGEGTGRRRRRRRGRDRG
                     DGSESTSPEVEVEPVEVQGLLDLREDGYGFLRVAGFVASREDVYVSVKQCRQLGLRKG
                     DYLSGLARPATHKEKNPALVRIETVNGLDPDAARDRVRFDDLTAIDPGDQLSIGTGNP
                     EDLTGELIDALAPVRLGARGLIVAPPNVDTSPVLTTVANAIEAAHPEVHVIVLLVDER
                     PETVTSFRSALEGGEVVSSTFDRPADQHAAVAELAIEHAKRLVEYGNDVVVLLDSLTG
                     LARAFNLAAAPDGRTIHGELDAGALGSAKRVFGTGRNVEDGGSLTVLATVLSDSGSNI
                     DDVILTELLRGANSALHLEPATVAEPPRKLPVGAAPQAQKALLAELNG"
     gene            1409..1720
                     /locus_tag="PLP95_02995"
     CDS             1409..1720
                     /locus_tag="PLP95_02995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007212861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HPB44797.1"
                     /translation="MKADIHPNYRNVVFQDTSSGDKFMTRSTVETSETVVWEDGNEYP
                     LAKVEISAFTHPFYTGQMKIIDTAGRVERFQKRYGDRVRRPSKAAAAEAPAAAEGADA
                     E"
     gene            1758..2810
                     /locus_tag="PLP95_03000"
     CDS             1758..2810
                     /locus_tag="PLP95_03000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB44798.1"
                     /translation="MNALPEERLGQLRVAKLKALLGERLDGDEVVNSSPFGVGAALAA
                     RSGLVAFLIEEADRRSLGGALVWALRQRASLPGPIRVVVIVDADEPTAGHLAREATWF
                     DVPVAVERIVGTASVPVAAAPFEDRGEAVEIPGEIHALVVGEVDDAASSGHRDVDVVV
                     EWGVLRVEVLGLEVARVVPGDSAGGVRLEIGIGKFDREIAAMMHAEVPPATALHNAVE
                     IVRTYRHAAAALHPLRDLVAERWLRQIALADPSLVGARELFPIDSTLQPQSLRESQPA
                     SARGVAADGSPIIAVFGAGVDLDIVPHAADTRALHDPDATLIIVMPQRNILAPVVAVA
                     DALVSPARFVNLELPY"
     gene            2885..3949
                     /gene="prfA"
                     /locus_tag="PLP95_03005"
     CDS             2885..3949
                     /gene="prfA"
                     /locus_tag="PLP95_03005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HPB44799.1"
                     /translation="MLERLAPLERELRDVDAMLGDPDVFADQARFVKLSKRRKELEPI
                     VERAVELRRASEDLDVAREMFSDLAGDDREAMREEIDVNEARIVELEAEIKILLLPRD
                     PNDDRNVIMEIRGAEGGEEANLFARDLFEMYRAYAQRQGWGFEVMDASVSDLGGYADV
                     TFVLKGDSVWSRMKHEAGPHRVQRVPVTESQGRVHTSSATVTVLPEAEEVDVAIDPND
                     LQIDVYRSSGPGGQSVNTTDSAVRITHKPTGLVVAMQDEKSQIQNRAKALVVLRSRLL
                     KLEQDRQAAELSSARKSQIGGGGRSEKIRTYNFKENRISDHRIGFTAYNLDKVLAGEL
                     DHVSDALVHHERAEQLEDLS"
     gene            3997..4920
                     /gene="prmC"
                     /locus_tag="PLP95_03010"
     CDS             3997..4920
                     /gene="prmC"
                     /locus_tag="PLP95_03010"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: protein motif:HMM:TIGR00536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HPB44800.1"
                     /translation="MGPHTDGPGDLAGTVAWSELLSEATARLRRAGLDTAASDARRIV
                     EEASGLEGAELIVSLEDPVTERAMARFDDMIARREAGEPLQYVLGRWGFRRLDLAVDP
                     RVLIPRPETEMLVEAALGHLDRLAARIAADGERRDVVVADLGTGSGAIALSVASERTN
                     TTVWACDASPAAVAAARANLAGIGRAAVRVRIVEGEWFDAFSDEQRSSFDLIITNPPY
                     VSIDDELPAEVAEWEPAVALFAGADGLDDLRHIISEAPRWLRPAGVLAVELDPRQVAA
                     VRDLMTGAGFTSTEVHQDLAGRDRIVIGRLG"
     gene            complement(4975..5775)
                     /locus_tag="PLP95_03015"
     CDS             complement(4975..5775)
                     /locus_tag="PLP95_03015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB44801.1"
                     /translation="MKLASRRIKCPLCGTINPPQSDRCSVCTRPLRESDLASQRVYDE
                     ALWSERIASKRARRPVSLATIAVVVVIVAAIVNYFVVKAGPDWAHEPRTIVPGETWHS
                     VDIGAGARLDLPGTPLVSNEVLGAVPATVSTVWVGDSWEAVLDDTTTSKIEFESALER
                     FRGAVLVATSTGDAATPPLAELTARVAAEAALTPSAVVPTEAAANQLSWRWESSATGW
                     PRPNVETTVAGRAIRSGNVTVSVVTIVAGDDGGALVDAVMANLQLPAA"
     gene            5917..6561
                     /locus_tag="PLP95_03020"
     CDS             5917..6561
                     /locus_tag="PLP95_03020"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: protein motif:HMM:TIGR00057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HPB44802.1"
                     /translation="MEEPQIDPVAPVVAVVGADQHGLECAAEALRSGSCVGLPTDTVY
                     GLSAALSRSGAVRSLFELKGRAATKAMAVLVGSVDQARELGVLNEHAVEIVQRYWPGP
                     LTVVVRRADGLRIDLGGDSGTIGLRCPDSTLLNALIAQVGPLVTTSANRAGHPTITSA
                     ARIAEEFGDRLAVVIDGGELAESASTVVDLSGTGIDVLREGPIPTGDLMRASRR"
BASE COUNT         1060 a         2217 c         2295 g         1045 t
ORIGIN      
        1 ccggcaccga cggtcgcagc cgacgatgcc acgggccctg cctcgggccc tgccgccggc
       61 gatacggcag gcgacggcca acccggcgaa cctctcggct ccgacaccgc cgatgtgtcc
      121 tcggccacga acggcgattc cgccgcggcc gcggctcgcg aagcgaccga cggtactacg
      181 ggcgctgcgg gagacgaaga ggcggcggac gaggggtcga ccgacggctc gaccgacggg
      241 tccgaagacg ccgcggggga gggcaccggc cgtcgccgcc gccgtcgccg cggccgcgat
      301 cgcggcgacg gatcggaatc cacatcgccc gaggtcgagg tggaaccggt cgaggtgcag
      361 ggcctgttgg accttcgcga ggacggctac gggttcttgc gcgtcgctgg cttcgtcgcc
      421 agccgtgagg atgtgtacgt ctcggtcaag caatgccgtc agctcggttt gcgcaagggc
      481 gactatctgt cgggcctcgc tcgacctgcg acgcacaagg aaaagaatcc ggcgctcgta
      541 cgcatcgaaa ccgtcaacgg tctcgacccg gacgcggcac gcgatcgggt ccgcttcgac
      601 gacctcacgg ccatcgaccc cggtgatcag ttgtcgatcg gtaccggcaa ccctgaggat
      661 ctcaccggcg aactcatcga tgcgctcgcc ccggtccgac tcggggctcg tggcctcatc
      721 gtcgctccgc cgaacgtcga caccagcccc gtgctcacca cggtcgccaa cgcgatcgag
      781 gcggcccacc ccgaggtgca cgtcatcgtg ctgctcgtcg acgagcgtcc cgagaccgtc
      841 acgagcttcc gctcggcgct cgagggcggg gaagtggtga gctcgacgtt cgatcgtccg
      901 gccgaccaac acgcagcggt cgccgagttg gcgatcgagc atgccaagcg cctcgtcgag
      961 tacggcaacg acgtcgtcgt cctcctcgac agcctcaccg gcctggcccg agcgttcaac
     1021 cttgcagcgg ccccggatgg ccgcacgatc cacggtgagc tcgatgccgg cgcactcggt
     1081 tcggcaaagc gtgtgttcgg caccggccgc aacgtggagg acggcggctc gctcaccgtg
     1141 ttggccacgg tgctgagcga cagcggctcg aacatcgacg acgtcatcct caccgagctg
     1201 cttcgcggcg ccaactcggc gctgcatctc gaaccggcca ccgtcgccga gccgccacgc
     1261 aagctgccgg tcggtgcggc gccgcaggcc caaaaggcgc tgctcgccga gctcaacggc
     1321 tgacgtccaa ttccctcctt cggcgtcgcc ccgatcgggg ccgtcgatgt tggcggctcg
     1381 gcgccgcctt gcctagtctg gagtaactat gaaggctgac atccatccca actaccgaaa
     1441 cgtcgtgttc caggacacgt cgtcgggcga caagttcatg acgcgctcga ccgtcgagac
     1501 ctccgagacg gtggtgtggg aagacggcaa cgagtacccg ctcgccaagg tggaaatctc
     1561 ggccttcacg caccccttct acaccggcca gatgaagatc atcgacaccg ccggtcgtgt
     1621 ggagcgtttc cagaagcgct acggcgatcg cgtgcgtcgc ccctcgaagg ccgctgccgc
     1681 cgaggcaccg gccgccgctg aaggcgccga cgcagagtga gcccggtacc cccggtaccg
     1741 gcaccacagg agcaacgatg aacgcactgc ccgaggagcg cctcgggcag ttgcgcgtcg
     1801 cgaaactcaa ggcgctgctc ggcgaacgac tcgacggtga cgaggtggtg aactcgtcac
     1861 cgttcggcgt cggagcggca ctggcggccc gctcgggact tgtcgcattt ctcatcgagg
     1921 aggccgaccg gcgcagcctc ggcggggcac tcgtgtgggc gttgcgccag cgggcgtcgc
     1981 ttcccggccc gattcgggtg gtggtgatcg tcgacgccga tgagcccacc gcgggtcacc
     2041 tggcccgcga ggcgacctgg ttcgatgtgc cggtcgcggt ggaacgcatc gtcggcaccg
     2101 cctcggtgcc ggtcgccgcg gcgccgttcg aggaccgtgg cgaggccgtg gagatccccg
     2161 gtgagatcca tgcgctcgtc gttggcgagg tggacgacgc cgcgagttcg gggcaccgtg
     2221 acgtcgacgt cgtcgtcgag tggggagtgt tgcgcgtcga agtgctcggc ctcgaggtcg
     2281 cccgggtggt gcccggcgat tctgcgggtg gggtgcgact cgagatcggg atcggcaagt
     2341 tcgaccgcga gatcgcagcg atgatgcacg ccgaggtacc tccggccacc gcgcttcaca
     2401 acgcggtgga gatcgtgcgc acctatcggc atgccgcagc ggcgctgcac ccgctgcgcg
     2461 atctcgtcgc cgagcgttgg ctgcgtcaga tcgcactggc cgacccgtcg ctggtcgggg
     2521 cccgcgaatt gttccccatc gactcgaccc tgcagccgca gagtcttcgt gaatcccaac
     2581 cggcgtccgc ccgcggtgtc gccgcggacg ggtcgccgat catcgccgtg ttcggcgccg
     2641 gtgtcgacct cgatatcgtc ccccacgccg ccgacacccg agcgttgcac gaccctgacg
     2701 cgacgctgat catcgtcatg cctcagcgca acatcctcgc tccggttgtc gcagtggcag
     2761 atgcgctcgt gtcgcccgcc cgcttcgtca acctcgaact cccgtactga gacccccgta
     2821 ctgatggctc cccgcccgat ggccccgcgg cgtgagaccc ccaaaccgac cgaaggtgca
     2881 caccgtgctc gaacgactcg ccccactcga acgtgaactc cgcgacgtgg atgccatgct
     2941 gggcgatccc gacgttttcg ccgatcaggc ccggttcgtg aagttgtcga aacgccgcaa
     3001 ggagctcgaa ccgatcgtcg agcgagcggt cgagttgcga cgcgcgagcg aggacctcga
     3061 cgtggcgcgg gagatgttca gcgatctcgc cggcgatgat cgcgaagcga tgcgcgagga
     3121 gatcgacgtc aacgaggctc gaatcgtcga actcgaggcg gagatcaaga tcctgctgtt
     3181 accccgcgat cccaacgacg atcgcaacgt catcatggag atccgcggcg ccgagggtgg
     3241 cgaagaagcc aacctgttcg ctcgcgacct gttcgagatg taccgggcct acgcccagcg
     3301 ccaagggtgg ggctttgagg tgatggacgc ttcggtgtcg gatctcggtg gctatgccga
     3361 cgtgacgttc gtgctcaagg gggacagcgt ctggtcgcgc atgaaacacg aggccggccc
     3421 tcaccgcgtt cagcgggtgc cggtgaccga gagccagggc cgggtacaca cgagttcggc
     3481 cacggtcacg gtgttacccg aggcagagga ggtcgacgtg gcgatcgacc ccaacgacct
     3541 gcagatcgac gtctaccgca gctccggtcc cggcgggcag tcggtgaaca ccaccgactc
     3601 ggcggtgcgc atcacccaca agcccaccgg gctcgtggtg gcgatgcagg acgagaagtc
     3661 gcagattcag aatcgggcaa aggcgctggt ggtgttgcgc agccgtctgt tgaagctcga
     3721 acaggaccga caggccgccg aattgtcgag cgctcgcaag agccagatcg gcgggggcgg
     3781 acgaagcgag aagatccgga cctacaactt caaggagaac cggatctcgg atcaccgaat
     3841 cgggttcacc gcctacaacc tcgacaaggt gctcgcgggc gaactcgatc acgtcagcga
     3901 cgcgctggtt caccatgagc gggccgagca actcgaagac ctgtcgtgaa ccgctctgag
     3961 ccgactgagc cgactgagcc gaccgaggag ccggacatgg gtccgcacac cgacggacct
     4021 ggcgatctgg cgggcaccgt cgcctggtcc gagttgttgt ccgaggcgac ggcgcggctg
     4081 cgtcgagcgg ggctcgacac cgccgcgagc gatgcccggc gcatcgtcga agaggcctcc
     4141 ggtctggagg gggccgagct catcgtgtcg ttggaggatc ccgtgaccga acgggccatg
     4201 gcacgcttcg acgacatgat cgctcggcgc gaggcgggcg agccgctgca gtacgtgttg
     4261 ggacggtggg ggtttcgtcg actcgacctg gcggtcgacc ctcgagtgct catcccccgc
     4321 ccagagaccg agatgctcgt ggaggcggcg ctcggccacc tcgatcggct cgccgcccgg
     4381 atcgcagcgg acggcgagcg acgcgacgtc gtggtcgccg acctcggcac cggctcaggg
     4441 gcgatcgcgt tgtcggtggc ctcggaacgg accaacacca ccgtgtgggc ctgcgatgca
     4501 tcaccagcgg cggtcgcagc ggcgcgggcg aatctcgcag gcatcggacg ggcggcggtg
     4561 cgcgtccgca tcgtcgaggg ggagtggttt gacgccttca gcgatgagca gcgttcgagt
     4621 ttcgacctca tcatcaccaa cccgccctat gtgtccatcg acgacgagtt gcccgccgag
     4681 gtcgctgagt gggaacccgc cgtagcgctg ttcgccggtg ccgatgggct cgacgatctt
     4741 cgccacatca tcagcgaggc tcctcggtgg ttgcgccccg cgggcgtgtt agcggtcgaa
     4801 ctcgaccctc gacaggtcgc tgcggtgcgc gacctcatga cgggcgccgg gttcacctcc
     4861 accgaggttc accaggatct cgccgggcgc gatcggatcg tgatcggtcg gctcggatag
     4921 ggcggctcgg agaggtcggc gctgatcgat cgagagcgcc gatctccggc cgggtcaggc
     4981 cgcgggcaac tgcaggttcg ccatgacggc gtccacaagc gcgccaccgt cgtcgccggc
     5041 gacgatggtc accaccgaca ccgtgacgtt gccggaccgg atcgcccggc ctgccacggt
     5101 cgtttcgaca ttcgggcgtg gccaaccggt ggccgaggac tcccaccgcc acgagagttg
     5161 attcgccgcg gcttcggtgg gcacgacggc cgacggggtg agcgcggcct ccgcggccac
     5221 ccgggcggtc aactcggcca acgggggagt tgcggcgtcc ccggtactgg tggcgacgag
     5281 caccgcgccg cggaaccgtt cgagggcgct ttcgaattcg atcttggagg tggtcgtgtc
     5341 gtcgaggacc gcttcccacg aatctccgac ccacaccgtc gagaccgtcg cgggcaccgc
     5401 accgagcact tcgttgctca ccagcggggt tccgggaagg tcgaggcgcg ccccggcacc
     5461 gatgtcgacg ctgtgccagg tttcgcccgg cacgatcgtg cgcggctcat gggcccagtc
     5521 cggtccagcc ttgacgacga agtagttcac gatcgcggcg acgatgacca ccacgacggc
     5581 gatcgtcgcg aggctcacag gccggcgagc ccgcttcgag gcgatccgtt ccgaccacag
     5641 cgcttcgtcg tagacgcgct gagatgccag gtcggactcc ctgagcggac gcgtgcacac
     5701 cgaacaccga tccgactgcg gggggttgat cgttccgcac aacgggcact tgatccgtcg
     5761 agatgccagc ttcatcgtcg ctccttcacc cctcacttat cggcgttggc tccctggagt
     5821 tgagtcgacg cacctcgggt ggtggagcga cggtctcgcc acgtccggtc gggccggcgc
     5881 tcgcgcgccg gcgttcgcgt actaccgtcg gcggtgatgg aagaacccca gatcgacccg
     5941 gtggcgcccg tcgttgcggt ggtgggtgcc gaccagcacg gcctcgaatg cgctgcggag
     6001 gcgctgcggt cggggtcgtg tgtcgggctg ccgaccgaca ccgtctatgg actgtcggcg
     6061 gcgttgtcgc ggtcgggggc cgtgcggtcg ttgttcgaac tcaagggccg tgccgcgacc
     6121 aaggccatgg cggtgctggt cggatcggtc gaccaggcgc gggagctcgg cgtgctgaat
     6181 gagcacgcgg tggagatcgt gcaacggtac tggcctggcc cgctcacggt ggtggtgcga
     6241 cgagccgacg gcctccgaat cgatctcgga ggagattccg gaacgatcgg attgcggtgt
     6301 ccggattcga cgctgttgaa cgcgctcatc gcccaggtcg gacctctcgt cacgacgagc
     6361 gcaaaccgtg ccggacaccc gacgatcacc agcgccgcga gaatcgccga ggagttcggt
     6421 gaccgattgg ccgtcgtgat cgacggtggc gagttggccg agtcggcgtc gacggtggtc
     6481 gatctgtccg gaacgggcat cgatgtgttg cgcgaggggc cgattcccac gggtgacctg
     6541 atgcgggcgt cgcgtcgctg acgtcgcgtc gcaaacgctt ctggccacgg cactcgctag
     6601 agtcctgcga tgacggg
//
LOCUS       DAOOMA010000065         6465 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_14278_length_6465_cov_4.196568, whole genome shotgun sequence.
ACCESSION   DAOOMA010000065 DAOOMA010000000
VERSION     DAOOMA010000065.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 6465)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6465)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6465
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14278_length_6465_cov_4.196568"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..521
                     /locus_tag="PLP95_03025"
     CDS             <1..521
                     /locus_tag="PLP95_03025"
                     /inference="COORDINATES: protein motif:HMM:NF014794.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HPB44803.1"
                     /translation="ASATVLSNRGANGIDGVTSTAVGVALASQRPTVCLIGDVAFLHD
                     TNAMLGLASRAAPLCVVVIDNDGGGIFSFLPQADQLDGERFEQLFGTPHRTHLLGLAG
                     AHRIETIDIGSVERTPAIEELREVVRAFRERPRPLVVRVRSDRAVNVAAHRDLNDRVA
                     AAARAALGPTGG"
     gene            complement(528..1742)
                     /locus_tag="PLP95_03030"
     CDS             complement(528..1742)
                     /locus_tag="PLP95_03030"
                     /EC_number="5.4.4.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008909 - isochorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase"
                     /protein_id="HPB44804.1"
                     /translation="MLTRRAERDERDGPDSRPATSFLSPRRELRGWGEAWRISVPAPW
                     VDHLDTVSEALSSIEHRDLDSTAESDRCTSHPHGSGPVAFAALPFDRSAPAEFIVPSW
                     LLGTTPDGQRWVTHLDGLEQPEPTPEFPAPRSLSLSTPMTAAAWKESVAAATRRIAAG
                     ELDKVVLSRALEVIADVAFDPHVIAARLATAHPHAYRYDIDGFVGATPELLVSRFDDV
                     VRAHPMAGTTPRTGDPTIDSAQASELLHSPKNRGEHQITIDMVLDTLLGFTSFVDAAP
                     APSVVAAGSVQHLATEVEGRLSEPAASVLDLVAALHPTPAVGGRPRDTALALISELEG
                     DDRGPYAGPVGWVDARGNGAWAVALRGAHIEGNRARLRAGVGVVADSDPDSEFAETQA
                     KFTSVLAAITRL"
     gene            complement(1782..2588)
                     /locus_tag="PLP95_03035"
     CDS             complement(1782..2588)
                     /locus_tag="PLP95_03035"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012228871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone/menaquinone biosynthesis
                     methyltransferase"
                     /protein_id="HPB44805.1"
                     /translation="MTPRTTSENAPENAPENASSVAPSNASSVAPSNASSVALDPALP
                     QGEDKARAVQEMFDTIAPRYDLVNRIMTFRMDVGWRKRTVSALGLVPGSVVADVACGT
                     GDFCHELSAKGFVPVGVDFSFGMLAAAPKPFPRVQADVLALPFADGAIDGITCGFALR
                     NFVTLDGFFDELARAVRPGGRIALVDATTPPNPIMKFGHGIYFGKVVPLIGGLISDKA
                     AYAYLPKSLAYMPEPDEVLAMLRAAGFGDAERRLLSGGIAHLFTGTRNRD"
     gene            complement(2599..4368)
                     /locus_tag="PLP95_03040"
     CDS             complement(2599..4368)
                     /locus_tag="PLP95_03040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HPB44806.1"
                     /translation="MAAPFLTARQKRWDDRLESRSGHRRRYRRIYQGEIASMEFNLAQ
                     VFDALADALDDRVCIKTAKVTRTYAEMGERTSRFANALRARGLSVHAERSDLANHESG
                     QDHVALYLHNCPEYLEGMIGSYKARAVPFNVNYRYVAEELEYLFRDSNAKAVVFHSRF
                     APTLADIRDRLPNLTVFFQVRDESENDLLEGAEWYEDALAEASPEIPEELRASWSPDD
                     LYILYTGGTTGMPKGVLWRQADIFCAAMGGRPYGSADELESVDAVVKQALGGGITILP
                     APPFMHGAAHWTAFICFTQGGTIALQGVVDRLDAVDMLTSIEQLGVNTIVLVGDAFAR
                     PIIDEIERGDYDLSSLFVLTNGGAAMSVGVKSKLLELLPTAMIIDGIGSSETGQQAYQ
                     VSSAGSEIASGTFSPTPGACVINDTFDALISPDDDTVGWLGQRGRVPLGYLGDADKTS
                     RTFPVIDGVRYSIPGDRAHYTLTGPIEVLGRDSVTINSGGEKIFAEEVEAALAPHADV
                     FDVVVCGRPSERWGNEVTAIVQLREGAAADEASLLAEAQRHIARYKLPKAFVFVDKIE
                     RSPSGKADYRWAKGLAADDLNAS"
     gene            complement(4434..5921)
                     /locus_tag="PLP95_03045"
     CDS             complement(4434..5921)
                     /locus_tag="PLP95_03045"
                     /inference="COORDINATES: protein motif:HMM:NF012770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HPB44807.1"
                     /translation="MLPTAVVALVTFVALVASACTGGSDEATASAPDTTSVSTVASGD
                     IDGWTPDPLVWRTCESSRRHHCATLVVPFDWSDPDGETIELAVARLPSTGANVGSLIM
                     NPGGPGGSGVQFLTGSDFNDTLTDSFDLVSWDPRGVGDSTALSCAGPDTLEEFLANDP
                     SPDDEAERAALDQDARRIADDCSGSGSPQADLAKHIGTDNVARDLEALRLALGDESLN
                     YLGFSYGTMVGLRYLAAFPTHVRAMVLDAVVDPTEHLETWLLNQATAFETALQATFDE
                     CTTKCPVDDLAAAYDELHALVERAPVPAGNGRKLGPAELETAAIYISYSGDASMEFAR
                     AVSEALNDGDGTSMYEIAQGYYDLSGYAAYAAVECVDSEHPVGSEAYAAFTDRVIAAA
                     PRLGASVASELLPCAFWNAPVASVSGPVTGDGSPPILVVGNTGDAATPYSNATNVADM
                     LTNGVLVTYHGNGHTSYGRSSCIDTIVERYLIDLEVPTQNPQCGK"
BASE COUNT          982 a         1960 c         2443 g         1080 t
ORIGIN      
        1 gcgcttcggc gacggtgctg tcgaaccgtg gggccaacgg catcgatggg gtcacctcca
       61 ccgccgtggg agttgccctc gcctcgcagc gcccgacggt gtgcctgatc ggagacgttg
      121 cgttcctcca cgacaccaac gcgatgctcg ggcttgcgtc gcgggcggcg ccgttgtgcg
      181 tcgtggtgat cgacaacgac ggcggcggca tcttttcgtt cctgccccag gcggatcagc
      241 tcgacggcga gcgtttcgag cagctcttcg ggaccccgca tcgaacgcat ctgctcggac
      301 tcgccggggc gcaccgcatc gaaacgatcg acatcggcag cgtggagcgc actcctgcga
      361 tcgaggaact tcgagaggtc gtgcgggcgt ttcgtgaacg gccccgcccg ctcgtcgtgc
      421 gggtgcgcag cgaccgggcc gtcaacgttg ccgcccaccg ggacctgaac gaccgggtcg
      481 cggcggcggc gcgtgcggcg ctggggccta caggcgggtg atggccgcta cagacgggtg
      541 atcgccgcga gcaccgaggt gaacttcgcc tgggtttcgg cgaactcgct gtcggggtcc
      601 gagtcggcca ccacgcccac ccccgcacgt aggcgcgctc ggttgccttc gatgtgggct
      661 cctcgaagcg cgaccgccca tgcgccgttg ccccgggcat cgacccagcc gaccgggccg
      721 gcataggggc cgcggtcgtc gccttcgagt tcggagatga gggcgagcgc cgtgtcgcgg
      781 gggcgtccgc cgaccgcggg ggtgggatgc agggcggcga cgaggtcgag caccgaggcc
      841 gccggctcgc tgaggcgccc ctcgacctcg gtggcgaggt gttgcacgct gccggcggcg
      901 accaccgacg gggcaggggc ggcgtcgacg aagctggtga accccaacag ggtgtcgagc
      961 accatgtcga tggtgatctg atgttcgccg cggttcttgg gagagtgcag cagttctgag
     1021 gcctgggccg agtcgatcgt cgggtcgccg gttcggggcg tggtgccggc catcgggtgg
     1081 gcccgaacga catcgtcgaa gcgggagacc aacagttccg gcgtggcgcc gacgaacccg
     1141 tcgatgtcgt agcgataggc gtgcggatgg gcagtcgcga gccgggcggc gatcacgtgg
     1201 gggtcgaagg cgacatcggc gatcacctcg agggcccgtg acagcacgac cttgtcgagt
     1261 tcgccggccg cgatgcgtcg ggtcgccgcg gcgaccgact ccttccacgc cgcggcggtc
     1321 atcggcgtgc tcaacgacag cgaccgcggc gccgggaact cgggtgtcgg ctcgggctgt
     1381 tcgagcccgt cgaggtgggt cacccagcgc tgcccgtcgg gggtggtgcc gagcagccag
     1441 gagggcacga tgaactcggc cggcgcggac cggtcgaagg gcaatgcggc gaaggccacc
     1501 ggtccggacc cgtgtgggtg cgaggtgcag cggtccgact cggcggtgga gtccaggtct
     1561 cggtgctcga tcgaggacag cgcctcgctc accgtgtcga ggtgatcgac ccacggtgcg
     1621 ggaaccgaaa tacgccacgc ctcaccccag ccgcgcagct cgcggcgcgg cgacaagaaa
     1681 ctggtcgccg gccgtgagtc ggggccgtcg cgttcatccc gctcggcgcg ccgagtcaac
     1741 attaccggtg gggtcgacgc aggggagggg gccgacacgg ctcagtcccg gttgcgggtg
     1801 cccgtgaaca ggtgcgcgat accgccgctg agcagccgcc gctcggcgtc gccgaaccct
     1861 gcggctcgca acatggcgag cacctcgtcc ggctcgggca tgtaggcgag cgacttcggg
     1921 aggtaggcgt aggcggcctt gtcggagatc agcccgccga tgagcggcac gaccttgccg
     1981 aagtagatgc cgtggccgaa cttcatgatg gggttcggcg gggtggtggc gtcgacgagt
     2041 gcaatgcggc ctccggggcg cacggcgcgg gccaactcgt cgaagaaccc gtcgagggtg
     2101 acgaaattgc gtagggcgaa tccacacgtg atgccgtcga tggcgccatc cgcgaagggc
     2161 agcgccaaca cgtccgcctg aacccgaggg aacggcttcg gagcggccgc taacatgccg
     2221 aagctgaaat cgacaccgac ggggacgaaa cctttcgcgc tgagctcatg gcagaaatca
     2281 ccggttccgc aggccacgtc ggccacgacc gaaccgggga cgaggccgag ggccgaaacg
     2341 gtgcgcttac gccacccgac atccatgcga aaggtcatga tgcggttgac gaggtcgtat
     2401 cgcggggcga tcgtgtcgaa catctcctga acggcgcggg ccttgtcctc gccctggggc
     2461 agcgcagggt cgagcgcgac gctcgaagcg ttcgacggcg cgacgctcga agcgttcgac
     2521 ggcgcgacgc tcgaagcgtt ctcgggcgcg ttctcgggag cgttctcaga cgtggttcgg
     2581 ggagtcacct ccgcgagcct aggaggcgtt gaggtcgtcg gccgcgagcc ccttcgccca
     2641 ccggtagtcc gccttgccgg aaggggaccg ttcgatcttg tcgacgaaca cgaaggcctt
     2701 gggcagtttg tagcgggcga tgtggcgttg tgcctcggcg aggagcgagg cctcatccgc
     2761 ggcggcgcct tcgcgtagtt ggacgatggc ggtgacctcg ttgccccagc gttcgctcgg
     2821 tcggccgcac acgaccacgt cgaacacgtc ggcgtgaggg gcgagggcgg cctcgacctc
     2881 ttcagcgaag atcttctcgc ctccggagtt gatcgtcacc gaatcccggc cgagcacctc
     2941 gatcggcccc gtgagcgtgt agtgggcgcg gtcgcccgga atcgagtagc gcacgccgtc
     3001 gatgaccgga aaggtgcgcg aggtcttgtc ggcgtcgccg aggtagccga gcggcacgcg
     3061 accgcgttgg ccgagccagc cgaccgtgtc gtcgtcgggg gagatgagcg catcgaaggt
     3121 gtcgttgatc acgcaagcgc ccggggtcgg gctgaaggtc ccgctcgcga tctcgctgcc
     3181 ggcgctcgac acctggtacg cctgctggcc ggtctcggag ctgccgatgc cgtcgatgat
     3241 catcgcggtc ggcagcagtt cgagcagctt ggacttcacg ccgacactca tcgccgcgcc
     3301 gccgttggtc agcacgaaca gcgagctgag gtcgtaatcg ccccgttcga tctcgtcgat
     3361 gatcggccgg gcgaacgcgt cgcccaccag gacgatggtg ttgacgccga gttgttcgat
     3421 cgaggtgagc atgtcgacgg catcgaggcg gtcgacgacc ccctgaagcg cgatcgttcc
     3481 gccctgggtg aagcagatga aggccgtcca gtgggcggcg ccgtgcatga acggcggggc
     3541 cggcaggatc gtaattccgc cgccgagcgc ctgtttgacg acggcgtcca ccgactcgag
     3601 ttcgtcggcg cttccgtagg ggcggccacc cattgcggcg cagaagatgt cggcctggcg
     3661 ccacagaacg cccttgggca tgccggtggt gccgccggtg tagaggatgt agaggtcatc
     3721 gggagaccag gaggctcgca gttcctcggg gatttccggt gacgcttcgg cgagcgcgtc
     3781 ctcgtaccat tcggcgcctt cgagcaggtc gttttcggat tcgtcgcgga cctggaagaa
     3841 gacggtgagg ttggggaggc gatctcggat gtcggccagg gtgggcgcga agcgcgaatg
     3901 aaagacgacg gccttcgcgt tcgagtctcg gaagaggtac tcgagttcct cggccacgta
     3961 gcggtagttg acgttgaacg gcaccgcacg ggccttgtac gagccgatca tcccctcgag
     4021 gtattcgggg cagttgtgga gatacaacgc cacgtgatcc tggccggact cgtggttcgc
     4081 cagatcgctg cgctcggcgt gcacgctcag gccgcgggct cgcagtgcat tggcgaagcg
     4141 actggtccgt tcacccatct ccgcgtaggt gcgagtcacc ttcgcggtct tgatacagac
     4201 gcggtcgtcg agcgcgtctg ccagcgcatc gaacacctga gcgaggttga actccatgga
     4261 cgcgatctcc ccttgataga tgcggcggta gcgacggcgg tggccggacc gcgattctag
     4321 acgatcgtcc caccgtttct gacgggccgt cagaaacggt gcggccacgg cagcggtcga
     4381 gcaactcgcc gggtttacga ttagcgctca cctgcagcgc tcacctgcag ggctcacttc
     4441 ccacattgcg gattctgggt cggaacctcg aggtcgatga ggtaacgctc gacgatggtg
     4501 tcgatgcacg acgagcgacc ataggaggtg tgaccgttgc cgtggtaggt gacgagcacg
     4561 ccgttagtca gcatgtcggc gacgttcgtt gcgttcgagt acggggtggc ggcatcgccg
     4621 gtgttgccga cgacgaggat cggcggcgat ccgtcgccgg tgacggggcc cgatacggag
     4681 gcgacggggg cgttccagaa ggcacagggc aacagctccg acgcgacgga ggcgccgagc
     4741 cgaggcgcgg cggcgatgac tcggtcggtg aatgcggcgt aggcctcgga accgacgggg
     4801 tgttcggagt cgacgcactc caccgctgcg taggcggcgt acccggacag gtcgtagtac
     4861 ccctgggcga tctcgtacat ggaggttccg tcgccatcgt tgagcgcttc gctgacggcc
     4921 cgggcgaatt ccatcgaggc gtcgccgcta taggagatgt agatcgcagc ggtttcgagt
     4981 tcggcgggtc cgagttttcg gccgttgccg gcggggaccg gtgctcgttc gacgagggcg
     5041 tggagctcgt cgtaggcggc ggcgaggtcg tcgacggggc acttggttgt gcactcgtcg
     5101 aaggtggcct ggagcgcggt ttcgaatgct gtggcctggt tgaggagcca ggtttcgagg
     5161 tgctcggtcg ggtcgaccac ggcgtcgagg accatcgctc gcacgtgggt tgggaacgct
     5221 gcgaggtagc gcaggcccac catggtgccg taggagaacc cgaggtagtt gagcgactcg
     5281 tcgccgaggg cgaggcgcag cgcttcgagg tcgcgggcga cgttgtcggt gccgatgtgc
     5341 ttcgcgagat cggcctgagg tgatccggat ccgctgcaat cgtccgcgat gcgtcgggca
     5401 tcttggtcga gtgcggcgcg ttcggcctcg tcgtcggggc tggggtcgtt cgcgaggaac
     5461 tcctcgagcg tgtctggtcc ggcgcagctc agcgctgtgg agtctccgac gccacgaggg
     5521 tcccagctca cgaggtcgaa tgagtcggtg agcgtgtcgt tgaagtctga tccagtgagg
     5581 aattgcacgc cgctgccgcc gggcccgccc ggattcatga tgagcgaccc gacgttggcg
     5641 cccgtagagg gcagccgggc gaccgcgagt tcgatggttt cgccatcggg gtcggaccag
     5701 tcgaacggaa cgaccagcgt ggcgcagtgg tggcggcgac tcgactcgca cgtgcgccaa
     5761 accagcgggt cgggggtcca gccgtcgatg tcgccgctcg cgacggtgga aaccgacgtg
     5821 gtgtcggggg ccgaggcggt cgcctcgtcc gagccgcccg tgcatgccga agcgacgagg
     5881 gcaacgaagg tgacgagggc gacgaccgcg gtgggcagca cggagaacgc ggagacgcgt
     5941 ggggtggcgt gtatggaggc gcgtgcagtg gcgtgagtgg tgtcgtgagt ggccgagcgg
     6001 actggagtcg gagtgggacc gagggcctcc gtcgggacgg gagcggtggt tggcgggtgc
     6061 gacgtcaaac ggtcgggacg gggactcggc acgcgcacaa ccctatgagg aacgatcatc
     6121 acgtcaacac gcgggccggt cgggttcggg cgcgcgtcgg tgtcagcgcc tttaccgacg
     6181 cttggtgggg agactccggt gggtgggcca ttgacgtgtc ggttcattgg cgtatcggtg
     6241 gttggggtgg tttggtcggg gtgagtgaga gttgcccgac tgtggggcgc ctggccctcg
     6301 tcgctcctcc ggttcggctg ctcctgcccg agtttcggcc tgtgtttgac ctgcgggcgt
     6361 cgtggggtcg gtagatgcgg gtgtcggggg tggcggccat gcggtcgtaa cggcgccgga
     6421 tggtggcgat cgcttcggcc aaatcgacgg ttccgccgcg gactt
//
LOCUS       DAOOMA010000066         6456 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_14305_length_6456_cov_4.719419, whole genome shotgun sequence.
ACCESSION   DAOOMA010000066 DAOOMA010000000
VERSION     DAOOMA010000066.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 6456)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6456)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6456
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14305_length_6456_cov_4.719419"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..202
                     /locus_tag="PLP95_03050"
     CDS             <1..202
                     /locus_tag="PLP95_03050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB44808.1"
                     /translation="AQAGVPVLIVAGEVFDDALVRARAITPDVEVIDLVETVGRQRAM
                     TETGAAITDAVIDWLGGSTMPR"
     gene            199..1650
                     /locus_tag="PLP95_03055"
     CDS             199..1650
                     /locus_tag="PLP95_03055"
                     /inference="COORDINATES: protein motif:HMM:NF013584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HPB44809.1"
                     /translation="MNPFAALDVTDQARLVKRREVSPTELVTAAIEAVEAVNPALNAV
                     IHERFERALGEAANMSRTAPFAGVPIVLKDLDGTLADEPYHAGTKHLRLHGYRATATS
                     ELFARLQAAGFIVIAKTNTPEFGLVPTTEPESYGPTRNPWNRSHSTGGSSGGSAAAVA
                     SGMVAVGHAGDGGGSIRIPASECGLVGLKPTRGRIPLGPVETEAWGGLVARLVLTRSV
                     RDTAAILDAVSAPRLGEVYGAPQPERPYVAELNVAHRQLRIGYTTQSADGTPTDPEVA
                     ASIERTAQVLASLGHHVTEAAPRQIGDEAFFAELSGHFLGLFPVWTAQSVDELESLTG
                     VSATPETVEPATWALAEAGRAISGVDFANSMHRLRQRCGEIEQWWAEGFDLLLTATIP
                     ELPPTLGQFGATEDNPLNGIFRATPIVANTMPFNMTGQPAMSVPATPSSSGLPIGAQL
                     IAAFGRDDLAVRVAAELEVAQPWADRRPEVWAG"
     gene            complement(1691..3124)
                     /locus_tag="PLP95_03060"
     CDS             complement(1691..3124)
                     /locus_tag="PLP95_03060"
                     /inference="COORDINATES: protein motif:HMM:NF012975.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNB domain-containing ribonuclease"
                     /protein_id="HPB44810.1"
                     /translation="MLGPRQHITDSLNTEFAALREEVGVRDAFPAEVLELAATRAQRP
                     SDFFDSHLDRRDLEFSSIDPPGARDLDQAFHIASDGDGFRLHYAIADMGSWVDLDDAI
                     DVEARQRGTTIYSPDRRIPLHPPTLSEGAASLLEGVDRPAIVWDLRIDSAGITTAIDV
                     ERCLVRNRRALTYEHVQRELDSGSPDEQFVLLRRLGEALIDAEQARGGVSLELPEQSV
                     TLVDGHYELRYDAALDVESFNAQLSLATGRAAAQLMIDAGFGILRTLPRADPSVFDSL
                     RLSARSLGADWPDSMSYPEWVRTLNPRTAEGAALMNLAGRTLRGSSYLAFTDGLSNLD
                     GDPYAHEHAAIAAPYAHVTAPLRRLVDRFANECVLAAAAGEEPSPRLREAIGEIPSTM
                     GRTMQVASRVTKASIDLAEAAAMLHLVGHTFDAMVISSARGSATLQLRHPAVIASVDV
                     ELPIGHDVRVRLEAASLDGPVMRFRPV"
     gene            complement(3134..3898)
                     /locus_tag="PLP95_03065"
     CDS             complement(3134..3898)
                     /locus_tag="PLP95_03065"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: protein motif:HMM:NF013007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HPB44811.1"
                     /translation="MMLFGVVNCSSDSLNTDSYVRDEAEARTRIDTLLVDGAQGVDIG
                     GQGSTDRAILADWKTEWTRVEPVIAAALERTSDVSIDSWRPEVVRRALEAGANTINAA
                     NGMQNDTFWELAATFEAAVVVPFMNGPNPLELRHVDGDPIDAMIAYFTERLATADRFG
                     VRSRCLLDPGTGFGPHGWQWEDRYHYQKHVYSNLERLRVFDLPLYIPLPWKRTAQHDE
                     LLEIVLARRPDYGRAHYPAHIRSVEASVVGSATPNA"
     gene            3938..4834
                     /locus_tag="PLP95_03070"
     CDS             3938..4834
                     /locus_tag="PLP95_03070"
                     /EC_number="2.4.1.266"
                     /inference="COORDINATES: protein motif:HMM:NF010496.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyl-3-phosphoglycerate synthase"
                     /protein_id="HPB44812.1"
                     /translation="MTGAPQRLSHDRAEVVRRVISRKGETRVSVCLPCRNEADSVGEV
                     VRALLREDNARDLIDELIVIDDGSTDDSARLAEAAGATVVAISQIHDRYGAGRGKGNV
                     LWASMLASTGELVVWIDADVRTVDLSWVAGLVEPMLEDPQVALVKATYHRPQDEGGGG
                     RTTELVIRPLMSLLASELAWLAQPLSGEVGVRRSMIEAVPFAQGWGVEIAMILDVADT
                     FGPGALAQADLGERRHRHHDLLDLRLQATEVMATFLSRRSVELPDDDPALVTPDGERR
                     LLNLAERPPIRSVRDASDQLRP"
     gene            4927..5205
                     /locus_tag="PLP95_03075"
     CDS             4927..5205
                     /locus_tag="PLP95_03075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012224946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HPB44813.1"
                     /translation="MSVTVRIPTTLRTLTGGANKVSVDGSTVAEVLANLDAAHPGFAT
                     RILDEDGNIARFVNVFVSDDDVRFMDGVNTEVPDGGEVSIIPAVAGGC"
     gene            complement(5230..5832)
                     /locus_tag="PLP95_03080"
     CDS             complement(5230..5832)
                     /locus_tag="PLP95_03080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB44814.1"
                     /translation="MNDVADSTSDRSWARLLRTPTGMALIGAVVLAGASHLIFGGWHW
                     VPIPLAVITVGLLADCVSGARPSVSVRMSVVTVASIVALLAAVTMTTDDLSGKPGTAD
                     STVDVALGPAATTTPEAAVEAATLDRDSWERLVACESALEQIRVAISSTPGFVAPEAV
                     AAAGSAAGSATGASEAEEYSELRLRSCEVRLSAIASSLQP"
     gene            6038..>6456
                     /locus_tag="PLP95_03085"
     CDS             6038..>6456
                     /locus_tag="PLP95_03085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012224954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TCP-1/cpn60 chaperonin family protein"
                     /protein_id="HPB44815.1"
                     /translation="MAKQITFDDEARRKLEAGMNTLADAVRVTLGPKGRNVVLDKKWG
                     APTITNDGVSIAKEIELEDPLENMGAALVKEVAKKTDDVAGDGTTTATVLAWSMVREG
                     LRNLAAGANPMSVKRGIEAAVAAAVEKIVEVSIEVET"
BASE COUNT         1026 a         2061 c         2323 g         1046 t
ORIGIN      
        1 cgcccaggcc ggggtcccgg tgttgatcgt cgccggggag gtgttcgacg acgcgctcgt
       61 ccgtgcccgc gccatcaccc cagatgtcga ggtcatcgac ctcgtggaga ccgtggggcg
      121 ccagcgcgcg atgaccgaga ccggcgcggc gatcaccgac gccgtcatcg actggctcgg
      181 tggatctacg atgccgcgat gaacccgttt gccgcgctcg atgtcaccga ccaggcccgt
      241 ctcgtgaaac gccgcgaggt gtcgccgacg gagctggtca ccgctgcgat cgaggcggtc
      301 gaagcggtga atccggcgtt gaacgcggtg atccatgagc ggttcgaacg tgccctgggc
      361 gaggcggcga acatgtcgcg gacggctccg tttgcggggg ttccgatcgt cttgaaggac
      421 ctcgacggca cgttggccga cgagccgtat cacgccggca ccaagcacct gcggcttcac
      481 ggctatcggg ccaccgcgac gagcgaactg ttcgcacggc tgcaggcggc ggggttcatc
      541 gtcatcgcca agaccaacac tccggagttc ggcctggttc cgacgaccga gccagagtcc
      601 tacggcccga cacggaaccc gtggaatcgt tcgcactcga ccggggggtc ctcgggcggg
      661 tcggcggcgg ccgtggcctc gggaatggtg gcggtgggcc acgccggcga tggcggcggc
      721 tcgattcgaa ttccggcgag cgaatgcggc ctcgtcggac tcaaaccgac gcgtggtcgc
      781 atccccctcg gtccggtcga aaccgaggcc tggggaggtc tggtcgcccg attggtgctc
      841 acccgttcgg tgcgcgacac cgcagcgatc ctcgatgccg tcagcgcccc gcgcctcggc
      901 gaggtctacg gagccccgca gcccgagcgg ccctatgtgg ccgagttgaa cgtcgcccac
      961 cgccagcttc gaatcggcta cacgacccag agcgccgacg gaacccccac cgaccccgag
     1021 gtcgcggcct cgatcgagcg caccgcccag gtgttggcat cgttgggcca ccatgtcacc
     1081 gaggcggcgc cgcgccagat cggcgacgaa gcgttcttcg ccgagctgtc ggggcacttc
     1141 ctcgggttgt ttccggtctg gacggcgcag tcggtggacg agctggaatc cctcaccggt
     1201 gtgtcggcca cccccgaaac ggtcgaaccg gcgacgtggg cgttggccga ggcggggcgt
     1261 gcgatcagcg gggtcgactt cgccaactcg atgcatcgac ttcgccagcg ctgcggagag
     1321 atcgaacagt ggtgggccga ggggttcgac ctgttgttga ccgcgacgat tcccgagttg
     1381 cctccgaccc tcggacagtt cggcgcgacc gaggacaacc cgttgaacgg gatcttccgg
     1441 gcgaccccga tcgtggcgaa cacgatgccg ttcaacatga ccggccaacc ggcgatgtcg
     1501 gtgccggcga cgccgtcgag cagcgggttg ccgatcggtg cgcaactcat cgctgcgttc
     1561 gggcgcgacg atctggccgt gcgcgtcgcc gctgagctcg aggtcgcaca gccctgggcg
     1621 gatcggcgtc ccgaggtgtg ggccggctga gcacgtgtcg gccggctgag cacgtgtggg
     1681 ccggctgagc tcagaccggg cgaaaacgca tgaccggccc atcgagggag gccgcttcga
     1741 gccggacgcg cacgtcgtgg ccgatcggta gctcgacgtc gaccgaggcg atcaccgccg
     1801 ggtgccgcag ctgcaacgtg gccgagcccc gggccgagga gatcaccatc gcatcgaagg
     1861 tgtggccgac cagatgcaac atcgcggccg cctccgccag atcgatcgag gccttggtga
     1921 cccgcgacgc aacctgcatg gtgcgcccca tcgtcgaggg gatctcgccg atcgcctccc
     1981 gcagtcgcgg cgacggctcc tcgccggcgg ctgcggcgag cacgcactcg ttcgcgaacc
     2041 gatcgacgag acgccgcagt ggagcggtca cgtgggcgta cggagcggcg atcgccgcgt
     2101 gctcgtgggc gtatgggtcg ccgtcgaggt tcgacagccc atcggtgaac gcgagatagc
     2161 tcgacccgcg cagcgtccgt ccggccaggt tcatgagggc ggcgccttcg gcggtacggg
     2221 ggttgagggt gcgcacccac tcgggatagc tcatcgagtc gggccaatcg gcgccgagcg
     2281 aacgggcact gagcctcagc gagtcgaaca ccgaagggtc tgctctggga agggttcgca
     2341 ggatgccgaa ccctgcgtcg atcatgagtt gcgccgcggc ccgcccggtg gcgagagaca
     2401 gctgcgcgtt gaaggactcg acgtcgagcg cggcgtcgta gcgcagttcg taatgaccgt
     2461 cgacaagggt gaccgactgt tcggggagtt cgaggctgac tccgccgcgg gcttgttcgg
     2521 cgtcgatcag cgcctcgccg aggcgacgga ggaggacgaa ctgctcatcg ggggaacccg
     2581 agtcgagttc gcgctgcacg tgttcgtacg tgagcgctcg ccggttgcgc acgaggcaac
     2641 gctcgacgtc gatggcggtg gtgatgcccg ccgagtcgat gcggagatcc cacacgatcg
     2701 ccggccggtc gactccttcg agcagactcg ccgctccctc gctgagcgtc ggtgggtgga
     2761 gggggatccg ccggtcgggg gagtagatgg tcgtgccgcg ttggcgggct tcgacatcga
     2821 ttgcatcatc gaggtcgacc cacgaaccca tgtccgcgat cgcgtagtgc agccgaaacc
     2881 cgtcgccgtc ggaggcgatg tggaatgcct ggtcgaggtc gcgggctccg ggcgggtcga
     2941 tcgaggagaa ctcgaggtct cggcggtcga ggtgggagtc gaaaaagtcg ctcggccgct
     3001 gcgctctggt ggcggcgagt tcgaggacct cggcagggaa cgcgtcgcgt accccgacct
     3061 cctcgcgcag cgcggcgaac tcggtgttga gcgagtcggt gatgtgttgg cgcggtccga
     3121 gcatggagtc ggttcaggcg ttcggtgtgg cggatccgac cacggaggcc tcgacgctgc
     3181 ggatgtgcgc gggatagtgc gcccggccgt agtcgggcct cctggccaga acgatttcga
     3241 ggagctcgtc gtgttgggcc gtgcgcttcc acggcaatgg gatgtacagc ggcaggtcga
     3301 agacccggag tcgttcgagg ttcgaataca cgtgcttctg gtagtggtaa cggtcttccc
     3361 actgccaccc gtgcggtccg aaacccgtgc cggggtccag caggcaacgt gagcgcaccc
     3421 cgaaacgatc ggcggtcgcg aggcgttcgg tgaagtaggc gatcatggcg tcgatcgggt
     3481 cgccgtcgac gtgtcgcagc tcgagcgggt tgggaccgtt catgaacggc acgacgaccg
     3541 ccgcttcgaa tgtggccgcc agttcccaga aggtgtcgtt ctgcatcccg ttggcggcgt
     3601 tgatggtgtt ggccccggcc tcgagcgccc gtcgaacgac ctcgggacgc cagctgtcga
     3661 tcgacacgtc gctggtgcgt tcgagggccg cggcgatcac cggctcgacg cgggtccact
     3721 cggttttcca gtccgctagg atcgcacgat cagtgctgcc ttgtccgccg atatcgacgc
     3781 cctgtgcgcc gtcgacaagc agtgtgtcga tgcgagtacg tgcctccgct tcgtcacgaa
     3841 cgtacgaatc tgtattcagt gagtcggacg agcaattgac gacaccgaaa agcatcatgg
     3901 cggcgaccct accagcgggc cgaacgggga ccctccgatg acgggggctc cacaacggct
     3961 ttcgcacgac cgcgccgagg tcgtgcgccg agtgatctcg cgcaagggcg agaccagggt
     4021 gtcggtgtgt ctgccgtgcc gcaacgaggc cgattcggtc ggcgaggtcg tccgggcgct
     4081 gttgcgtgag gacaacgccc gtgacctcat cgacgagttg atcgtgatcg acgacggctc
     4141 gaccgacgac tcggcgcgac tcgccgaggc cgcgggtgcg accgtggtgg cgatttcgca
     4201 gatccacgat cggtacgggg cgggtcgcgg caagggcaac gtgttgtggg cgtcgatgct
     4261 ggcgagcacc ggcgagctgg tggtgtggat cgacgcggac gttcgcacgg tcgacctgag
     4321 ctgggttgcc ggactcgtcg agccgatgtt ggaagatccc caggtagcgc tggtcaaggc
     4381 gacctatcac cgtccccagg atgagggcgg cggggggcgc acgaccgagt tggtcattcg
     4441 gccgctcatg tcgttgctcg cctccgagct cgcctggctg gcccagccgc tgtcgggcga
     4501 ggtgggggtg cggcgttcga tgatcgaagc cgtcccgttc gcgcaggggt ggggagtgga
     4561 gatcgcgatg atcctcgatg tcgccgacac ctttgggccg ggggcgctgg cgcaggcgga
     4621 tctcggtgag cgtcgccacc gtcaccacga cctgctcgac ctgcgcctgc aggcgaccga
     4681 ggtgatggcg acgttcctgt cgcgccgcag cgtcgagttg cccgacgacg atccggcgtt
     4741 ggtcaccccc gatggtgaac gacggctgct gaacctggcc gagcgcccac cgattcgatc
     4801 ggtccgggac gcgtcggatc agctccggcc ctgagggcgg gaatgtcggc cggtcccggt
     4861 tggttgggac ggtcaccgcg atcgctttag gatcgacggc tgcccaccac cgaaggattc
     4921 cacacgatga gcgttaccgt tcgcatcccg accactctgc gcaccctgac cggcggtgcg
     4981 aacaaggtga gcgtcgacgg ctccaccgtc gccgaggtgc tcgcgaacct cgatgcggcg
     5041 caccccggct ttgccacccg catcctcgac gaggacggca acatcgcacg gttcgtcaac
     5101 gtgttcgtgt ccgacgacga cgtgcgtttc atggacggcg tgaacaccga ggtgcccgac
     5161 ggcggtgagg tgtcgatcat cccggcggtc gccggcggct gctgaggctg cagtgctgag
     5221 gctgcagtgt tagggctgca gtgacgaggc gatcgcgctg agccgcacct cgcagctgcg
     5281 cagtcgcagc tccgagtact cctccgcttc gctggcgccg gtcgcggagc cggctgcgga
     5341 gccggctgcg gcgacggcct cgggcgcgac gaatccgggg gtcgaggaga tcgcgacgcg
     5401 gatctgttcg agggccgatt cgcacgcgac gagccgttcc caactgtcgc gatcgagggt
     5461 tgcggcttcc accgccgcct ccggtgtggt ggtggcggcc ggaccgaggg ccacgtcgac
     5521 tgtgctgtcg gcggtacccg gcttgccact caggtcatcg gtggtcatcg tgaccgcggc
     5581 gagaagggcc acgatcgagg cgacggtgac gaccgacatg cgcacgctga cgctcggtcg
     5641 agcgccgctc acgcagtccg ccaacaggcc gacggtgatg acggcgagcg ggatcggcac
     5701 ccagtgccac ccgccgaaga tcaaatggct ggcaccggcc aggacgacgg ctccgatcag
     5761 cgccatgccg gtcggtgttc gaagcaggcg tgcccacgat cggtcggacg ttgagtcggc
     5821 cacgtcgttc atgtcgtcct atcggccgtt gtcgggaggt cgtgagccaa tcctgagggt
     5881 gaggcgcggt cgtggagcag gggtggttag cactcgaccg gtacgagtgc taaaacaatc
     5941 agcactcgta ccggtcgagt gctgatttcg gggccacggt ctcgatccgc ccgccggtcg
     6001 tccactcccg actcatcacc acaaggattg agcagacatg gcaaagcaga tcacgttcga
     6061 cgacgaggcg cgtcgcaagc tcgaggccgg catgaacacg ctcgcggatg ccgtgcgcgt
     6121 cacgcttggc cccaagggtc gcaacgtcgt gctcgacaag aagtggggcg cccccacgat
     6181 caccaacgac ggcgtttcga tcgccaagga aatcgagctg gaagacccgc tcgagaacat
     6241 gggcgccgcg ctcgtcaagg aagtcgccaa gaagaccgac gacgtcgctg gcgacggcac
     6301 cacgacagcg acggtgctcg cctggtcgat ggtgcgcgaa ggcctgcgca acctcgccgc
     6361 cggtgccaac ccgatgtcgg tcaagcgggg catcgaggcc gccgtggccg ctgcggtcga
     6421 aaagatcgtc gaggtgtcca tcgaggttga gaccga
//
LOCUS       DAOOMA010000067         6440 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_14358_length_6440_cov_3.876116, whole genome shotgun sequence.
ACCESSION   DAOOMA010000067 DAOOMA010000000
VERSION     DAOOMA010000067.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 6440)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6440)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6440
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14358_length_6440_cov_3.876116"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            1..114
                     /gene="rpmF"
                     /locus_tag="PLP95_03090"
     CDS             1..114
                     /gene="rpmF"
                     /locus_tag="PLP95_03090"
                     /inference="COORDINATES: protein motif:HMM:TIGR01031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HPB44816.1"
                     /translation="MSAPPQSVCPRCNITKLPHVVCGNCGWYHGRQAIEVD"
     gene            253..540
                     /locus_tag="PLP95_03095"
     CDS             253..540
                     /locus_tag="PLP95_03095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantetheine-binding protein"
                     /protein_id="HPB44817.1"
                     /translation="MPAETHTHSAPLDRSRVLEIVQDLLGEILEIDPGSVSESDSLVE
                     DLHADSIAQYQLVEALISEFGERTVSDFETDEFDDIELVSDAVDYVVARLG"
     gene            518..1312
                     /gene="rnc"
                     /locus_tag="PLP95_03100"
     CDS             518..1312
                     /gene="rnc"
                     /locus_tag="PLP95_03100"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR02191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HPB44818.1"
                     /translation="MSSRDLADGGRAARLEALSEQLGYVFGSPDLLEAALLHRSWCAE
                     NGGVPSNERLEFLGDAVLQLVITDRLFSAEPPLPEGVLAQRRAALVNTRTLAGAARRV
                     DLGGVIRLGRGEASTGGADKDSILADATEAVLGAVYLDGGLDVATEVTTRWLSGDIER
                     IEAGGDLGDAKSKLQELTAHGLEAVPHYEVWGSGPEHALDFQARVRLVPAEIDPADFD
                     VAVATGAAATVWGEGLGRTKKDAEQAAATAALKRLVDEGKLNDEHA"
     gene            1305..2132
                     /locus_tag="PLP95_03105"
     CDS             1305..2132
                     /locus_tag="PLP95_03105"
                     /inference="COORDINATES: protein motif:HMM:NF018533.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase family
                     protein"
                     /protein_id="HPB44819.1"
                     /translation="MPELPELEILRRDLDKEVTGKKIKSVEITGTQTTKRGGTKKSLL
                     ARLDGHKIDSVSRKGTWIVLGLDSGEKAMISLGAGAGLRRNQAKDAPQPGLAVNITFT
                     QGGQLRLLDPKKSCEFFVVGEDEVADVVGEIGFDLVEEAIPWTKFGEQLLRRNAKLKA
                     ILMDPTFLVGIGPMYSDEILFESGLRYDRTPEGLSTQELRRLSRAAVEIVHDSIKHGG
                     ASVGPDGWVNLHGEAGSYNEFINVHHRDGEMSPRARGPIVKAKFQGAYTYFCEQTQV"
     gene            2164..5646
                     /gene="smc"
                     /locus_tag="PLP95_03110"
     CDS             2164..5646
                     /gene="smc"
                     /locus_tag="PLP95_03110"
                     /inference="COORDINATES: protein motif:HMM:TIGR02168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HPB44820.1"
                     /translation="MFLKTLQIRGFKSFADVASLQLEPGVTVVVGPNGSGKSNVVDAI
                     GWVLGAQAPSAVRSQKMDDVIFAGSAKRPALGRAEVSLTIDNTSATLPIEFNEVTITR
                     TLFRSGDSEYQINGVSCRLLDIQELLSDSGVGRQQHVIISQGQIDAVLNAKPEDRRLI
                     IEEAAGVLKFRRRKEKSERRLNATEGNLLRIQDLLREVRRQLRPLERQADAARRHEAV
                     ITELTALRLHLVGRELFRLRQQLQESTRTQNELRTTDREIRRRLAGLDAEILATEAQL
                     SAMGGDDFGDALVRYESLRERAKGMQSLLIERRRGIERDRNAFVDQAVVATLEAEHAQ
                     LTGELAQVVADEEATISNTAALEAAEVELAEARREFESDWGDGVAPPPSEAAEIRGEL
                     AALRATMERTEADSRRLNTQRESARTKLERIVDEAQRLVTQHAEAEANVAQFGTSAER
                     TERERAEAESAIVEVEERAANASAELHSWTARAEALSLALDDARKKAGAKHLAGLDGV
                     VGTLLDIVEVDEGWQSAFEAAAGEALSAVVVADGTTGRAALAALNNNTLSGAVLAITQ
                     AVSQTRGAEVSAGIGEALRQHVKGLSPDVEPLLDALLSGVVVVDGDWTAALDAALAHP
                     DATIVSRSGDRFGRTGWRVGQTGTGATGAALVEAQEHLGTAREAAEQTAAELKLAKER
                     LAQLRRDANDAEKADDQNTSRVRSLADALRRTERDRVESEREMATVDQHLADLSERIE
                     RESARRTELEQRLPALEAVEAEMSERARKMAEARRELEAKASRVGALRTDVQVKVKGL
                     ADRKRFLTERIAQVDERLSRNQAERLAAEQRRLQLDARAEVTDVLVAFVADRLVEVEA
                     GLEVLRDARRRQSEKTRAVTATLEDLRRSRAGAERELEENRERMQRAEIADAETRMRL
                     EAATEQVRTELDAEPEVAIRAECPELPDGATPQARLRELERDLKLMGPINPLALQEFE
                     ALQERHQFLESQLEDIRSTRKELTKVIRAIDEEIVNVFAAAYADVAMNFKELFELLFP
                     GGQGGLTLTDPNDLLSTGIEIEARPSGKNVRKLSLLSGGERSLVALAYLFAVFRSRPS
                     PFYVMDEVEAALDDVNLHRFLSLVAEFRSTAQLIIVSHQKRTMEAADVLYGVTMEPGG
                     SSKVLSEKVGAARS"
     gene            complement(5747..6223)
                     /locus_tag="PLP95_03115"
     CDS             complement(5747..6223)
                     /locus_tag="PLP95_03115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB44821.1"
                     /translation="MKRLIGALAVAAILFTTGCGGDDDSFPEDEFVRQVTANGVTEDV
                     ARCTYDGIRDDADIMAELRRTGGPNDQISDAAARTMSRTIAACLLGLELDEDGQPISA
                     VTTTTASPSKSTTTEPAGESRATTTARDGATSETGRTTTTRASDRTTTSLGTRSGN"
BASE COUNT         1108 a         2059 c         2255 g         1018 t
ORIGIN      
        1 ctgtccgctc cgccgcagag tgtctgcccg cgctgcaaca tcaccaagtt gccccacgtc
       61 gtgtgcggta actgcgggtg gtaccacggt cgtcaggcga tcgaggtcga ctgatccaaa
      121 tccccacccc aggtggcacc ggcgcgataa cattccgccc gtcacacccg gtggcgcggc
      181 gcgttgaggc gacgaggcgg accgcaccac ggtgtttccc gtccgagcac accgccgtag
      241 aggagatcgt cgatgccagc cgaaacgcat acgcattccg ctcccctcga ccggtcccgg
      301 gtgttggaaa tcgtgcagga cctgctgggt gagatcctcg agatcgaccc gggctcggtc
      361 tccgagtcgg actccctcgt cgaagacctt catgccgact cgatcgccca gtaccaactg
      421 gttgaggcgt tgatcagcga gttcggggag cgcaccgtat ccgactttga gaccgacgag
      481 ttcgacgaca tcgaactcgt cagtgacgcg gtcgactatg tcgtcgcgcg acttggctga
      541 cggggggcgc gccgcccggc tcgaggcgct gagcgagcag ctcgggtacg tgttcggttc
      601 gcccgacctg ctcgaagccg ccctgttgca ccggtcgtgg tgcgccgaga acggcggggt
      661 gcccagcaac gagcgactcg agttcttggg cgatgcggtg ttgcaattgg tcatcaccga
      721 ccggctcttt tccgccgaac cgccgctgcc cgagggggtg ctggcccagc gccgcgccgc
      781 cctggtcaac acccgaaccc tcgccggagc ggcccgccgg gtcgatctgg gtggggtcat
      841 ccggctcggt cggggagagg cctccaccgg tggcgccgac aaggattcga tcctcgccga
      901 tgccaccgag gcggtactcg gtgccgtcta cctcgacggt ggtctcgacg tggccaccga
      961 ggtgacgacg cgctggttgt ccggcgatat cgaacgaatc gaagccggtg gcgatctcgg
     1021 cgacgcgaag tcgaagcttc aggaactcac cgcccacggc ctcgaggcgg ttccccacta
     1081 cgaagtgtgg ggtagcggac ccgagcacgc cctcgacttc caggcacggg tacggctcgt
     1141 gcccgccgag atcgatcccg cagatttcga tgttgccgta gccaccggag cggcggcgac
     1201 ggtgtggggc gaggggctcg gccgcacgaa aaaggacgcc gaacaagcag ctgcgaccgc
     1261 tgccctgaaa cgactggtcg acgaagggaa actgaacgat gagcatgcct gagctgcccg
     1321 agctggagat ccttcggcga gacctcgaca aagaggtcac cggcaagaag atcaagtcgg
     1381 tggagatcac cggaactcag accaccaaac gcggcggaac gaagaagtcg ctgctggccc
     1441 gcctcgacgg acacaagatc gattcggtga gccgcaaggg aacctggatc gttctcgggc
     1501 tcgattcggg ggagaaggcg atgatttcgc tcggtgccgg cgccgggctt cggcgaaacc
     1561 aggccaaaga cgccccgcag ccgggtcttg cggtgaacat cacgttcacc cagggtgggc
     1621 agctgcgcct gctcgacccg aagaagtcgt gtgagttttt cgtcgtcggc gaggacgagg
     1681 tggccgacgt ggtcggcgag atcggcttcg acctggtgga ggaggcgatc ccgtggacca
     1741 agttcggtga gcagctgcta cgacgcaacg ccaagctgaa ggcgatactg atggacccga
     1801 cgttcctcgt cggcatcgga ccgatgtact ccgatgagat cctcttcgag tcgggcctgc
     1861 gctacgaccg cacccccgag gggctgtcga cccaggaact tcgtcgcctc agccgtgctg
     1921 cggtcgagat cgtgcacgat tcgatcaaac acggcggtgc ctcggtcggg ccggacggct
     1981 gggtcaacct ccacggcgag gccgggtcgt acaacgaatt catcaacgtt caccatcgcg
     2041 acggcgaaat gagccctcgt gcccgcggcc cgatcgtgaa ggcgaagttc caaggcgcct
     2101 acacgtattt ctgcgagcag acccaggtct gagcggctca ggtcgtcgct gtaggctcgc
     2161 ttcgtgttcc tcaagacgct gcagatcagg ggcttcaagt cctttgctga tgtggcctcg
     2221 cttcaactcg aacccggggt caccgtcgtg gtcggcccca acggttccgg caagtcgaac
     2281 gtggtcgatg cgatcggctg ggtgctcggt gcccaggcgc cctctgcggt gcgttcccag
     2341 aagatggatg acgtcatttt cgccgggtcg gcgaaacggc cggctctcgg tcgcgccgag
     2401 gtcagcctca cgatcgacaa cacctcggcc accctgccga tcgagttcaa cgaggtcacc
     2461 atcacccgca ccctgtttcg ttcgggtgac agcgaatacc agatcaacgg cgtgtcgtgt
     2521 cgattgctcg acattcagga gcttctgagc gactcggggg tcgggcgcca acaacacgtc
     2581 atcatcagcc agggtcagat cgatgcggtg ctgaacgcca agccggagga ccggcgcctc
     2641 atcatcgagg aggcggccgg ggtgttgaaa ttccggcgtc gcaaggagaa gtccgaacgg
     2701 cgcctcaacg ccaccgaggg caacctgttg cgcatccagg acctgttgcg cgaggtgcgc
     2761 cgccagcttc gtccgctcga acgccaggcc gatgcggccc gccgccacga agcggtgatc
     2821 accgagttga cggcgttgcg gttgcacctc gtgggtcgcg aactgtttcg gctccgccag
     2881 cagttgcagg agtcgacccg cactcagaac gagcttcgca ccaccgaccg cgaaattcgg
     2941 cgccgcctcg ccggcctcga cgccgagatt ctcgcgaccg aggcgcaact gtcggcgatg
     3001 ggcggcgacg acttcggcga tgcgctcgtt cgctacgagt cgttgcgtga acgcgccaag
     3061 gggatgcagt cgttgctgat cgagcggcgc cgtggcatcg aacgcgatcg caacgcgttc
     3121 gtcgatcagg cggtcgtggc gaccctcgaa gccgaacacg cacagttgac gggtgagttg
     3181 gcccaggtgg tcgccgatga ggaggcgacc atctccaaca cggccgcgct cgaggccgcc
     3241 gaagtggagt tggccgaggc ccgccgcgaa ttcgagtccg actggggcga cggtgttgct
     3301 ccgccgccga gcgaggccgc ggagatccga ggcgagctcg cagcgttgcg cgccaccatg
     3361 gagcgcaccg aggccgacag ccgccggctc aacacccaac gcgagagcgc ccgcaccaag
     3421 ctcgagcgca tcgtcgacga ggcgcaacgc ctcgtcaccc agcacgccga ggcggaggcg
     3481 aacgttgcac agttcgggac gagcgccgag cggaccgaac gagaacgggc cgaggcggag
     3541 tccgcgatcg tcgaggtcga ggagcgcgcc gcgaacgcgt cggccgaact gcacagctgg
     3601 accgcacggg ccgaggcatt gtccctcgcc ctcgacgacg cccgtaagaa ggccggtgcg
     3661 aagcacctcg ccggactcga tggggtcgtg ggcaccctgc tcgacatcgt cgaggtcgac
     3721 gaagggtggc agagcgcatt cgaggcggcg gcgggcgagg cgctgagcgc ggtggtcgta
     3781 gccgacggca ccaccgggcg tgcggcgttg gcggcgctga acaacaacac gttgtccgga
     3841 gcggttctgg ccattaccca ggcggtgagt cagacccgcg gcgccgaggt gtccgccgga
     3901 atcggcgagg cgttgcgtca acacgtcaag gggctgagcc cggatgtgga gccgctgctc
     3961 gacgcgctgt tgagcggggt ggtggtcgtc gacggcgact ggaccgctgc cctcgacgca
     4021 gcgctggcac atccggacgc gacgatcgtg agtcgaagtg gcgaccgctt cggccgtacc
     4081 ggttggcggg tcggccaaac cggaaccggg gcgaccggcg cggcgctggt cgaggcgcag
     4141 gagcaccttg gcaccgcacg cgaggcggcc gaacagaccg cggctgagtt gaaactcgcc
     4201 aaggaacgtc tggcccagtt gcgtcgcgac gccaacgatg ccgaaaaggc cgacgaccag
     4261 aacacctcgc gggtgcgcag cctggccgat gcgctccggc gtaccgaacg cgaccgggtc
     4321 gagtccgaac gagagatggc gacggtcgac cagcacctcg ccgacctctc cgagcgcatc
     4381 gagcgcgaat cggcgcggcg caccgaactc gaacagcggc taccagcgct cgaggcggtc
     4441 gaggcggaga tgtccgagcg ggcccggaag atggccgagg cgcggcgaga gctcgaggcg
     4501 aaggcctcgc gggtcggagc gctgcgcacc gacgtgcagg tcaaggtgaa gggtctcgcg
     4561 gatcgtaagc gcttcctcac cgagcgcatc gcgcaggtgg acgagcgctt gagccgcaac
     4621 caggccgagc gcctcgctgc cgaacagcgc cgcctgcaac tcgacgcccg cgccgaggtc
     4681 accgacgtgc tcgtcgcctt cgtcgccgat cgtctcgtcg aggtcgaagc cggcctggag
     4741 gtgttgcgag atgcccggcg gagacagtcc gaaaagaccc gtgcggtgac cgccacgctc
     4801 gaggatcttc gccggtcccg cgccggggcc gaacgagaat tggaggagaa ccgcgagcgg
     4861 atgcagcgcg ccgagatcgc ggacgccgag acccgcatga ggctcgaggc cgcgaccgag
     4921 caggtccgca ccgaactcga tgccgaaccc gaggtagcga ttcgcgccga gtgccccgaa
     4981 ctccccgacg gagcgacccc gcaggcccgc ctgcgtgaac tcgaacgcga cctcaagttg
     5041 atggggccga tcaacccgct cgcgctgcag gagttcgagg cgctccagga gcgccatcag
     5101 ttcctcgaga gccagctcga agacatccgc tcgacccgca aggaactcac gaaggtgatc
     5161 cgggcgatcg acgaggagat cgtcaacgtg ttcgccgccg cgtatgcgga tgtggcgatg
     5221 aacttcaagg aactgttcga gctgttgttc cccggcggcc agggtgggtt gacgctgacc
     5281 gacccgaacg acctgctgag caccggcatc gagatcgagg cgcggccctc gggcaagaac
     5341 gtccgcaagc tgtcgctgtt gtccggcggg gagcgctcgc tggtggcgtt ggcgtatctg
     5401 tttgcggtgt tccgcagccg cccgtcgccg ttctacgtga tggacgaggt ggaggccgcg
     5461 ctcgacgacg tgaacctgca ccggttcctg tcgctcgtgg ccgagttccg ttccaccgca
     5521 cagctgatca tcgtgagtca ccagaagcgc acgatggagg ccgcagacgt gctctacggc
     5581 gtgacgatgg aacctggcgg gtcctcgaag gtgctcagcg agaaggtcgg cgccgcacgc
     5641 tcctgaactg tgcgttgagt gcggtaccgg cggcggggac agcggcggag accgcggcgg
     5701 ggacgcggat ggtcctggac gcgccgcagc gttcgcagtg gcggcctcag ttgcccgaac
     5761 gcgttccgag cgaggtggtc gttcggtccg aggcgcgcgt ggtcgtggtt cgacccgtct
     5821 cagaggtggc cccgtcgcgt gcggtcgtcg tggctcgcga ttcgcccgcg ggttcggtgg
     5881 ttgtcgattt cgacggcgag gcggtggtcg tggtcacggc ggaaatgggt tggccgtcct
     5941 catcgagctc caggccgagg agacatgccg cgatggttct cgacatcgtg cgggccgcgg
     6001 cgtcggagat ctgatcgttc gggccgccgg ttcgtcgcag ctcggccatg atgtcggcgt
     6061 cgtcgcggat gccgtcgtag gtgcatcgag cgacgtcctc tgtgaccccg ttcgcggtga
     6121 cctgcctgac aaactcgtcc tcggggaatg agtcgtcgtc acccccgcat cccgttgtga
     6181 acaagattgc ggccaccgcg agcgcaccga tgagtcgctt catgggggcc aactgtatct
     6241 ggcccgaccc atggtgccgt gcctgagcac ccgctcgctt taggggcgcg ccgatgccca
     6301 cgcagttgcg ttgtggaccg agagttggag gttcagacgg ctgggcaggt ccgggcttgt
     6361 ccgggcttgt cctgaggagt cgcaatcgcc ggttcattgg cgtatcggtg gttggggtgg
     6421 tttggtcggg gtgagtgaga
//
LOCUS       DAOOMA010000068         6423 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_14407_length_6423_cov_3.406721, whole genome shotgun sequence.
ACCESSION   DAOOMA010000068 DAOOMA010000000
VERSION     DAOOMA010000068.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 6423)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6423)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6423
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14407_length_6423_cov_3.406721"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(765..2546)
                     /locus_tag="PLP95_03120"
     CDS             complement(765..2546)
                     /locus_tag="PLP95_03120"
                     /inference="COORDINATES: protein motif:HMM:NF012688.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase"
                     /protein_id="HPB44822.1"
                     /translation="MSEHLVDRLLASGARRGDAIGLATSRDGRVAISRDQVSADLDPA
                     SVAKTIAAIDAELAPRWVMWDQRTAATLVAHGIRLVRSWDLAAADRFVHGGWATSPAL
                     IWARLHDLDPNTIPVERGPDLFNQIDPAVESASPIDPDGYLRASWSAGGWEDFDGGAV
                     TWASLALRCARLQRAATDTLPDPPRTLSALRSESSIELLCSELAADGLPLDEARANEL
                     IAGYIGPRPTTQQDEFDARAQRDAAVFAAAPGVGQVDLRSPGQVRTLLRRVGIEVPDT
                     RAWRLKELRDRHPIVEALLTWRKAERIESTFGYRWLDENVSGGRLRGVWSGCDGAAGR
                     MTASAGLHNLPAELRDAVRAEPGYVFVRADLGQIEPRVLAVVSGDPKLAEATRDDDLY
                     APVAGALGVPRDVAKVAVLGAMYGQTTGIGAEALRGLNAAYPVAMAYLERADRSAQAA
                     NDLRTFGGRLIRLSASSPSPNGTEDRAAVAARGRFGRNAMVQGAAAEFFKVWALTVRA
                     RVAPLDARIVLCLHDELLVHTPAEYADKVAAAVDAALQEAAARWAPSSDGRSTTGRSD
                     AGRSTTGHSAVRFVAETSVVTSWADAK"
     gene            2649..3182
                     /locus_tag="PLP95_03125"
     CDS             2649..3182
                     /locus_tag="PLP95_03125"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: protein motif:HMM:TIGR00589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HPB44823.1"
                     /translation="MATQTSTKAAVLTIGYRVIDSPIGPLLVAASERGVHRIVLSEPD
                     HEFALCELERRVSPKVVEESLITEAVTRELGEFFAGERREFAVRSDLSLVEGFTRTVI
                     EALAHIPYGDRWSYGAVAEAIGHPRAHRAVGTACGANPVPLIHPCHRVIRSDGSVGSY
                     GGGEWMKRTLLDLETRR"
     gene            3214..3987
                     /locus_tag="PLP95_03130"
     CDS             3214..3987
                     /locus_tag="PLP95_03130"
                     /inference="COORDINATES: protein motif:HMM:NF024922.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoglutarate-dependent dioxygenase AlkB"
                     /protein_id="HPB44824.1"
                     /translation="MSLELFPLERSATHVAPGAVHVPDYLDESTQRRLVVEARDWMRP
                     PAPMRAVRLPGGGTMTVKGTCLGRYWVPYRYTDVAEDTDGAPVKPLPGWLAQLGADAV
                     AHAYRDQEWRRRYVPDAALVNFYEPGAQMGLHQDKEEQGDEPVVSFSVGASCIFRFGN
                     TQTRGRPWTDIELRSGDLFVFGGPSRLAYHGVPRLIAGTDRGDLGIASGRLNITLRMT
                     RLADRAPRMAQRATQRATRLATQPVVDPPPRDHATGGDQ"
     gene            3984..4628
                     /locus_tag="PLP95_03135"
     CDS             3984..4628
                     /locus_tag="PLP95_03135"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: protein motif:HMM:NF015319.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008725 - DNA-3-methyladenine glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HPB44825.1"
                     /translation="MTTSDRSTTSNPDHTPEPSPEPSPDLVAGSDGVLRCPWAASSPL
                     EQHYHDTEWGRELTGEREMFERIVLEAFQAGLSWRTVLNKREALRRAFVGFDVDAVAS
                     MSDDQLDALMADESIIRNRAKIRSARTNAIATIALRDGDGLNDLIWSFAPSVPTMRGA
                     VSEIPAVTAESEALANALKRAGFSFVGPTTAYALMQAVGMVNDHLIGCHRHDAG"
     gene            4743..5198
                     /locus_tag="PLP95_03140"
     CDS             4743..5198
                     /locus_tag="PLP95_03140"
                     /inference="COORDINATES: protein motif:HMM:NF016221.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016531 - copper chaperone activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone PCu(A)C"
                     /protein_id="HPB44826.1"
                     /translation="MIRSFVSLAVVTAMMGLAAGCGSSDPAIAVSGATIPVPMNPTVA
                     ALYFVINNSGGADVLTQVATDTGATATLHRSSMNPDGTSAMETLHEVPIDEGSSLAFK
                     PGGYHVMLDNPGDLAVGQQVEVTLRFKTSDPITVSAEVVESLTEESHGH"
     gene            5188..5916
                     /locus_tag="PLP95_03145"
     CDS             5188..5916
                     /locus_tag="PLP95_03145"
                     /inference="COORDINATES: protein motif:HMM:NF014667.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HPB44827.1"
                     /translation="MATENHPRFASTPRTRRQMLKSSMSLTGAAVLGATAFGSGVLSG
                     CSRSTASDVDVNQSPAAALGLHEDAILVGPLDKPTLTWTDHNSEPYNIAEATAGRTTL
                     MFFGYTSCPDQCPVYLNNMAAAIDTLEASARAVNVLFVGVDTARDTPEVMKNYLESKD
                     RNFLGLTASPAEIDGVLASLALPSVSFPDGTEGNYTVGHPLQCVAFTRDNLCHVMYPY
                     DLRQSEWADQLKVLSTIEWPAEES"
     gene            5913..>6423
                     /locus_tag="PLP95_03150"
     CDS             5913..>6423
                     /locus_tag="PLP95_03150"
                     /inference="COORDINATES: protein motif:HMM:NF021214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase assembly protein"
                     /protein_id="HPB44828.1"
                     /translation="MRFWCSNTGENWTWSWQPYWGAWIVVALLGFAYYRAGAFRPEHG
                     KGRRIGIVVGLVLLLAGIDWPLATLGAGYLITAQMVRQIIIVMIACPLLLWAAPPAAG
                     RALVATERRKAIYRVVSSPKFTLPVALGMLIAVTAPVVVDRLITSQIGSFLMDVIWIG
                     AGILIWLPVQ"
BASE COUNT         1082 a         2141 c         2083 g         1117 t
ORIGIN      
        1 aaccgggggc gggccaccct gacccacctc accaattccg cgacggacca ctaggtaatt
       61 tcctgaactg ggtatggcca agtcaatatc aatggtactt tgccgactcc ttccctgctg
      121 gaggggcgcc gaccgcctgt aatttcaata caggaagtgc cccatggacg gggtttggca
      181 actttctcgt tgtccgttcc aactcgccaa gatggtcaaa cggtggaacc gtaaagggca
      241 ttgcgcccac ctcggcgatc gagtaaaaga cttgggcgtg catggacggg ggcgcaacag
      301 cagcccgttt cactgcatgc accacccatt tctggcccaa tgatgtcaag tctggggaag
      361 cgtgttcgtc aatggagtgg ttccgtgcca atgtcatgaa ctcagcctac tcccggtatt
      421 tgaatcttgt ttcaatgggg gacctgtacc tgacgcgggt ggcaaactct gctcccgggg
      481 ttggcgggac gttgaaccaa tgcacagccg cgccaggaat gctgaacttc tggaagtcaa
      541 attttgccgc aggtggtcca gtcgacaatt caaggactct tggcgtgacc atgcccaagt
      601 cgatcacatc atcattgatc caggtcacgg gtttgcattc ggctcagggt tcacttatga
      661 ggtgtcgggg tgtcctgcgg gaaccacatt cgaacgttgc aatctgaccg atcatctgtt
      721 ggttcgggcg ctgccaaata tctagtggtc tgtcgctatc ctacttattt ggcgtcggcc
      781 caggaggtca cgacggaggt ttctgcgacg aaacgtacgg ctgaatgccc ggtggtggaa
      841 cgcccggcat cggaacggcc ggtggtggag cggccgtcgg agctcggcgc ccagcgcgcc
      901 gcggcctcct ggagcgccgc atcgaccgca gcggcgacct tgtcggcgta ctcggcgggt
      961 gtgtgcacca gcagttcgtc gtgaaggcac agcacgatcc gtgcgtcgag cggcgccacc
     1021 cgtgcgcgaa ccgtcaacgc ccacaccttg aagaactccg ccgccgcgcc ttgcaccatg
     1081 gcgttgcgcc cgaaacgtcc ccgggccgcg accgccgcgc gatcctcggt tccattcggg
     1141 gacggggacg acgcggaaag tcggatgagg cgtccgccga aggtgcgcag atcgttcgcc
     1201 gcttgagcac tgcgatccgc tcgttcgagg taggccatcg ccaccgggta ggcggcgttg
     1261 agccctcgca gggcctccgc tccgattccc gtggtttggc cgtacatcgc gccgagcacc
     1321 gccactttgg cgacatctcg cggcacaccg agtgctccgg ccaccggtgc gtacagatcg
     1381 tcgtcgcggg tggcttcggc gagtttcgga tcacccgaca ccaccgcgag gacgcgaggt
     1441 tcgatctggc cgaggtcggc gcgcacgaac acgtaccccg gctcggcgcg cacagcgtct
     1501 cgcaactccg ccgggagatt gtgcagcccg gccgacgcgg tcatacgccc ggccgccccg
     1561 tcgcatccgc tccacacccc acgaagacgc ccgccgctca cgttctcatc gagccatcga
     1621 tatccgaagg tgctctcgat gcgttcggct ttgcgccagg tgagcagcgc ctcgacgatc
     1681 gggtgccggt cgcgcagttc cttgaggcgc caagcccggg tgtcggggac ctcgatgccg
     1741 acgcgtcgca gcagagtgcg cacctggccg gggctgcgga ggtcgacttg gccaacgccg
     1801 ggtgcggcgg cgaagaccgc ggcgtcacgt tgggcacgtg cgtcgaactc gtcttgttgt
     1861 gtggtcgggc gggggccgat gtagccggcg atgagttcat tcgctcgggc ctcgtcgagc
     1921 gggagtccat ccgcggcgag ttccgagcag agcagctcga tcgacgactc gctgcgcaag
     1981 gcggacaggg ttcgtggcgg atcggggagt gtgtcggtag cggcgcgctg cagtcgtgca
     2041 catcgcaacg cgaggctggc ccacgtcacg gcgccgccgt cgaagtcctc ccaaccaccg
     2101 gcgctccacg acgcgcgcag atagccgtcg gggtcgatcg gcgatgcgga ctcgaccgct
     2161 ggatcgatct ggttgaacag gtcgggtccg cgctcgacgg ggatcgtgtt ggggtcgagg
     2221 tcgtgcagcc tcgcccagat gagcgcaggc gacgtggccc agccgccgtg tacgaatcgg
     2281 tcggcggcgg cgaggtccca ggagcgaaca aggcggatgc cgtgggcgac gagcgtggcc
     2341 gcggtgcgtt gatcccacat cacccagcga ggtgcgagct ctgcgtcgat ggcggcgatc
     2401 gttttggcga ccgaggccgg gtcgagatcc gcgctcacct gatctcggct gatggccacg
     2461 cgcccgtcac gagacgtcgc caacccgatc gcatcgccgc ggcgcgcccc tgaggcgagg
     2521 agtcgatcga cgaggtgctc agacatctcc cgccatcctg ccaccgcact gaggaaggtt
     2581 tttcaccatc cgcatggaca gacgaacaac cgttcgataa catcgaaccc gacgcaggag
     2641 gacgtgacat ggcaactcaa acgagcacca aggcggcagt gttgacgatc ggctatcggg
     2701 tgatcgactc gccgatcggg ccgttgctcg ttgctgccag cgaacgcggg gtacaccgca
     2761 tcgtgttgtc cgaacccgac cacgagtttg cgctgtgcga actcgagcgc agggtgagcc
     2821 ccaaggtggt ggaagagtcg ctgatcaccg aggccgtcac ccgagagctc ggcgagttct
     2881 tcgctggcga gcgccgcgag ttcgcggtcc gcagcgacct gtcgctggtc gaggggttca
     2941 cccggaccgt gatcgaagcc ttggcccaca tcccctacgg cgatcgctgg agctatggcg
     3001 cagtggccga ggcgatcgga catccgcgtg cacaccgggc agtcggcact gcctgtggtg
     3061 cgaacccggt gccgctcatc cacccgtgcc atcgggtgat tcgcagcgac ggcagcgtcg
     3121 ggtcctacgg cggaggcgag tggatgaagc gcacgctgct cgacctggaa acgcgccggt
     3181 aagcgcgggc atgacgggtg gtgacgggtg gtgatgtcgt tggaactgtt cccgcttgaa
     3241 cggagcgcca cacacgtagc gccgggtgcg gtgcacgtgc ccgactacct cgacgagtcg
     3301 acccagcgcc gactcgtcgt cgaggcccgg gactggatgc gcccgccggc gccgatgcgt
     3361 gcggtgcggc tcccaggcgg cgggaccatg accgtgaagg gcacctgtct aggtcgctac
     3421 tgggtgccgt atcgctacac cgacgtcgcc gaggacaccg acggcgcccc ggtcaagccc
     3481 ctgcccgggt ggctggcgca actcggtgcc gacgcggtcg cccatgcgta ccgcgaccag
     3541 gagtggaggc gccgctacgt tcccgacgcg gcactggtga acttctacga accgggtgcg
     3601 cagatggggc tccaccagga caaggaggaa cagggcgacg agccggtggt gtcgttcagc
     3661 gtcggggcct cgtgcatctt ccgattcgga aacacccaga cgcgtggccg gccgtggacc
     3721 gacatcgagt tgcggtcggg cgacctgttc gtcttcggcg gacccagtcg cctcgcgtat
     3781 cacggggttc cccgactcat cgccggcacc gaccgcggcg acctcggtat cgcctctgga
     3841 cggctcaaca tcaccctgcg aatgacgcgc ctcgcggatc gcgcgccacg catggcacaa
     3901 cgggcgaccc aacgggcgac ccgactggcg acccaaccgg tcgtcgatcc gcctccacgg
     3961 gaccacgcca caggaggcga ccaatgacca catccgaccg ttccacaaca tccaaccccg
     4021 accacacgcc cgaaccttca cccgagcctt cccccgatct cgtcgccgga tccgatggag
     4081 tgctgcgatg cccgtgggcg gcgtcgagtc cgctcgaaca gcactatcac gacaccgagt
     4141 ggggtcggga actcaccggc gaacgggaga tgttcgaacg catcgtgctc gaagcgttcc
     4201 aagcggggct cagttggcgc accgtgctga acaagcgcga ggcgctgcgg cgcgcctttg
     4261 tcggattcga cgtcgacgcc gtcgcgtcga tgagcgacga ccaactcgac gccctcatgg
     4321 ccgacgagtc gatcatccgc aaccgtgcca agatccgatc ggctcgcacc aatgccatcg
     4381 ccaccatcgc gttgcgcgac ggcgacggtc tcaacgacct catctggtcc ttcgctccgt
     4441 ctgtgccgac catgcgtggc gccgtgtcgg agatccccgc ggtcacagcg gagtcggagg
     4501 cgctggcgaa cgcgttgaaa cgcgccgggt tcagtttcgt cggcccgacc acggcctatg
     4561 ccctcatgca ggctgttggg atggtgaacg accacctcat cggctgtcat cgccacgatg
     4621 cgggttgaaa cgcgccgatc gggcctcgac ttggcagctt ctcgccggtc gactgaactg
     4681 gtgctgtgcg cgtctttgtt cggtcccccc accacgcgac acgcggatgc cgtgaaccac
     4741 gggtgatccg ctcgttcgtg tcgttggccg tcgtcaccgc gatgatgggt ctcgccgcag
     4801 ggtgcgggtc ctcggacccg gcgatcgcgg tcagtggtgc aaccatcccc gtaccgatga
     4861 acccgacggt ggcggcgctg tatttcgtca tcaacaactc gggcggtgcc gacgtgttga
     4921 cacaggtcgc caccgacacc ggagccaccg cgaccctgca ccgctcctcg atgaaccccg
     4981 acggcacctc cgccatggaa accctccacg aggttcccat cgacgagggt tcctcgctgg
     5041 ccttcaaacc gggcggctac cacgtcatgt tggacaaccc cggcgacctc gccgtgggcc
     5101 agcaggtcga ggtcaccttg aggttcaaga cctccgatcc gatcaccgtg tcggcagagg
     5161 tcgtggagtc gctgaccgag gagtcccatg gccactgaga atcacccacg gttcgcgtcg
     5221 accccacgga cccgacgaca gatgctcaag tcctcgatgt cgctgaccgg cgcagcggtg
     5281 ctcggggcga cggcgtttgg ttccggggtc ctgtcgggct gctcgcgctc aacggccagc
     5341 gatgtcgacg tcaaccaaag tccggccgca gcgctcgggc ttcacgagga cgcgatcctg
     5401 gtcgggccac tcgacaagcc gacgctgacc tggaccgacc acaactccga gccgtacaac
     5461 atcgccgagg ccaccgctgg gcgcaccacc ttgatgttct tcgggtacac gtcatgtccc
     5521 gatcagtgcc cggtctacct caacaacatg gcggcggcga tcgacaccct cgaagcctcc
     5581 gcacgggcgg tgaacgtgtt gttcgtcggc gtcgacaccg cccgcgacac ccccgaggtg
     5641 atgaagaact acctggagtc caaggatcgc aatttcctcg ggctcaccgc ctcgccggcc
     5701 gagatcgacg gtgtcctcgc ctcgctcgca ttgccgtcgg tcagcttccc cgacggcacc
     5761 gagggcaact acaccgtcgg gcacccgttg cagtgcgtcg cgttcacccg cgacaacctc
     5821 tgccacgtca tgtatcccta tgaccttcgt caatcggagt gggcggatca gctcaaggtg
     5881 ttgtccacga tcgaatggcc ggctgaggaa tcatgaggtt ctggtgctcc aacaccggtg
     5941 agaactggac ctggagttgg cagccctatt ggggcgcgtg gattgtcgtt gccctcctcg
     6001 gtttcgccta ctaccgggcg ggtgcctttc gtcccgaaca cggcaagggc cgacgcatcg
     6061 gcatcgtggt cggcttggtg ctactcctcg ccggaatcga ctggcccctg gccacgctcg
     6121 gggccggcta tctcatcacc gcgcagatgg tccgccagat catcatcgtg atgatcgcct
     6181 gtccgctgct gttgtgggcg gcaccgccag ccgctgggcg ggcactggtg gcgaccgagc
     6241 gccgcaaggc gatctaccgc gtggtgtcga gccccaagtt cacccttccg gtcgccctcg
     6301 ggatgctcat cgcggtcacg gcccccgtgg tcgtcgatcg cctgatcacc agccagatcg
     6361 gatcgttcct catggacgtg atctggatcg gcgccggcat cctcatctgg ttgcccgtgc
     6421 agc
//
LOCUS       DAOOMA010000069         6347 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_14612_length_6347_cov_4.570725, whole genome shotgun sequence.
ACCESSION   DAOOMA010000069 DAOOMA010000000
VERSION     DAOOMA010000069.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 6347)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6347)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6347
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14612_length_6347_cov_4.570725"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..570)
                     /locus_tag="PLP95_03155"
     CDS             complement(<1..570)
                     /locus_tag="PLP95_03155"
                     /inference="COORDINATES: protein motif:HMM:NF015686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB44829.1"
                     /translation="MPNLSTPTVHLLASSAPKDAFHVPAWLWASFLVGVAALLLIDLF
                     VLNKQAHEITTAEAARTSAGWIALGVGFAAVLPFVLDDGGTAAARYLTGYVVEKSLSV
                     DNVFVWAVIFRYFGVPSKYQHRVLFWGIFGALAMRAVFILAGAALLESFEWMMYVFGA
                     ILLYTAVNVFRDDEDEIDPEANPALRLVRR"
     gene            632..4135
                     /locus_tag="PLP95_03160"
     CDS             632..4135
                     /locus_tag="PLP95_03160"
                     /EC_number="3.1.11.5"
                     /inference="COORDINATES: protein motif:HMM:NF016169.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit gamma"
                     /protein_id="HPB44830.1"
                     /translation="MLEVVASQHIETLVADLARRLIDDPLDPMTPEWIAVGSDGLQRW
                     LQLELARHLGAQRGRDDGVAANITFAYPGTLRTAVVLAGTDHAEFDPWLQDRLVWSVL
                     ETLQNLSAGAVDAADGHTASILDSGGGASHALARRVASHFAAYQLHRPAMVMSWLEGH
                     DVDAAGSALAAPQLWQPELWRLVRDHLGVPSPPERLREALERVRRGEVDLMLRGRLLP
                     RRLVFFGPSLLPTGAGFLDVAEAVAQRPGTEVIVYVNEASSALSTTLRRELGDQKISQ
                     RRTHDVSAKVATHQLLRSWGRLQRETALLLAPYDTTVLPDEPRPASSVLGAVQADIRA
                     NREPAGTFAAAADDRSVQLHFCYGATRQVEAARDEICRLLADDDTLSEDDIVVLTPDP
                     TTFLPIVQAVFGASATRTRSHTADATPLIRYRISTGSATSNPAVTALLAMLNVATGRF
                     EVTEVLALLQMPAVRDRFGWTDDHIERISGWLADTNVHWGLDPAHRERFGLPAAITGY
                     SWQEALDQLLMGALVLGDDELAIGDVVPYDPASSEIDVIGQLGSVLSTLERLTEAATT
                     PRTIDEWCALLSDVIGETLGDTSPNGWHLDGAYRLLARLASTSRLARVGGERRADGTP
                     DSSTALLTLAEVHALASDTLGESRSRSDFFRGGITVTSLGALRNVPSRVTCVVGFDQA
                     AFGSGATDSDDLIAATPRIGDRDRRAEVRQTLLDAVMSTSDHFLTFSDGRDVQTNAEI
                     PPPVLLAELIETLCATLGVDDAEQLPAGILVRHPRHAFDAAYFTDDAGGLRSFNRSAL
                     RAALARRSRDPNATLDPDWPMIRLTEPMNPGGAALEIDTLRNVMRSPTRMFLEGGVGI
                     RLPRASEQLSARLPIEFDDLQKWELGTEVLDIERTGQNLHRWSEHQRRKGVLPPLGLA
                     DQIIGDLKTEVTSIIEEAQSRGLALVEGATVGIDLTIAGDRRILGSIVEVLSAPRRGT
                     AHVHFRRPKSAHLLEAWVDLLCLTAADPAVPWEVHLASRAPSSTSKPGPPPVAMVLRG
                     RDLGAVTAVDHLEFLVGLHDTAIRQPVPFFPELSRSITQGEDLGTQTKVWNEERGRLR
                     DERLVFGDHTLRQLREFTAEGHDIVANCDGETLLDRYANAVWSRFDQSVSVESDPAAN
                     DADASPPATDDEPPHSGGQKR"
     gene            4132..>6347
                     /locus_tag="PLP95_03165"
     CDS             4132..>6347
                     /locus_tag="PLP95_03165"
                     /inference="COORDINATES: protein motif:HMM:NF012789.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HPB44831.1"
                     /translation="MNELNSDPHGDTGEVFDTSAKLPTGRVCIEASAGTGKTFTLAAL
                     AVRSLAETDVPAAGLLIVTFTRAATAELRGRIRDAINEAITHLEAAIAALDTDQPAPQ
                     TDVAHLSAIAKGTLRELVERSRRLRVALTEYDTITITTIHAFAQQTLASLGVSSGTDS
                     LVISEADSNDAVIDACADVVARAIGEGHNPAMMPTLSKLADWTKLALRSSDLILEPAL
                     TDTDADDADQMRTRLVTEAVALLARRRHDRGLASFDDVLVALRDELRHATHAPERSAI
                     IDTLRSRYRIALIDEFQDTDPVQWEIFETIFDGDESTLVLVGDPKQAIYSFRGADIAT
                     YSRAITAPATRRTRLRTNWRADGIVIDALDELLRGATFGDGIDFVHVDPAEHNVSRSA
                     RRNDGATLSPVRIRLAINDDIERTGKTGTTQTDAVRLAIANDLVAQVHDALDHLVIPD
                     QDGDRAVQPDDLAVLVSTKAEAVAMQQALVASGVPAVLSRAEDVLESPSAIQWRWLLD
                     ALTRPSDPMRTRRFVLGWFSQRDITDLTADPDAVLDEAAERLATWNEVLRDRGVAEFV
                     RRIFDDSRVLERVLAGAEGDRAATDLQHLGELFQTQSTQRPGVNELLALLDPSGLDTE
                     HGEVGVASRRVETQQPSVQIMTVWVAKGLEYPVVFVPTLYSTPTKVDAICTKPDGTRR
                     LGLSEDLRKRYSADARLHRDTERLRLLYVAMTRGRHHCVLWWSPIHQNSRAPITRL"
BASE COUNT         1126 a         2269 c         1918 g         1034 t
ORIGIN      
        1 acggcgcacc agacgcaggg ccggattcgc ctcggggtcg atctcgtcct cgtcgtctcg
       61 aaacacgttg accgccgtgt agagcaggat cgctccgaat acgtacatca tccattcgaa
      121 cgactccagg agtgccgccc ccgcgaggat gaacaccgcc cgcattgcca gcgccccgaa
      181 aattccccag aacaacaccc ggtgctggta cttggacggg acaccgaagt accgaaagat
      241 caccgcccag acgaacacgt tgtcgaccga cagcgacttt tccaccacgt acccggtcag
      301 gtagcgcgcc gcagcggtgc ctccgtcgtc gagcacgaac ggcagcaccg ccgcaaagcc
      361 gacgccgagc gcgatccacc ccgcggaggt ccgtgccgcc tcggcggtgg tgatttcatg
      421 tgcctgtttg ttcaacacga acaggtcgat gagcaacaag gccgcgaccc cgacgagaaa
      481 cgatgcccac agccacgccg gaacgtgaaa cgcgtccttt ggcgccgacg aggcgagcag
      541 atgcacggtg ggcgtcgaca ggttcggcac gagcgacgat cgtaggtcgg tctcggtcga
      601 gtgtcagagg tcctcgttag ggtctgtgtt catgctcgaa gtcgtggcgt cgcaacacat
      661 cgaaacgctc gtcgccgatc tcgcgcgccg cctgatcgac gatccgctcg atccgatgac
      721 cccggaatgg atcgccgtcg gctccgacgg gctgcagcgc tggctgcaac tcgaactcgc
      781 gcgccacctg ggcgctcagc gcggccgcga cgacggagtg gcggccaaca tcaccttcgc
      841 ctatccgggc acgctgcgaa ccgccgtcgt gctcgccggc accgaccacg ccgagttcga
      901 cccctggctt caggatcgcc tcgtgtggtc ggtgctcgaa accctccaga acctttccgc
      961 cggcgccgtc gacgccgccg acgggcacac ggcgtcgatc ctcgactcag gcgggggagc
     1021 gtcacatgcg ctggcccggc gcgtcgcgtc gcacttcgcc gcctaccaac tgcatcgtcc
     1081 agccatggtg atgagttggc tcgagggtca cgacgtcgat gccgccggga gcgcactcgc
     1141 tgcaccacag ctctggcaac ccgaactgtg gcggttggtg cgcgatcacc tcggggtccc
     1201 gagtcctccg gagcgcttgc gggaggccct cgaacgggtt cgacggggcg aagtcgacct
     1261 catgttgcgc ggacgactgt tgccacgtcg cctggtgttc ttcggcccgt cactcctgcc
     1321 gaccggcgcg gggttcctcg acgtggccga ggccgtcgcg cagcgccccg gcaccgaggt
     1381 catcgtgtat gtcaacgagg cgtcgagcgc cctatccacc accctgcgcc gtgaactcgg
     1441 agaccagaag atctcgcaac gccgcaccca cgatgtgtcc gccaaggtgg caactcatca
     1501 actgcttcgg tcctggggtc ggctccaacg cgaaaccgcc ctgttgctcg ccccctacga
     1561 caccaccgtc ctgcccgatg aaccgcggcc cgccagctcg gtgctcggtg cggtgcaagc
     1621 cgacatccgc gccaaccgcg agcccgcggg caccttcgcg gccgcggccg acgatcgctc
     1681 cgtacagctg catttctgct acggagctac ccgacaggtc gaggccgctc gcgatgagat
     1741 ctgccggctt ctcgccgacg acgacacact gagcgaggac gacatcgtcg tgctcacccc
     1801 cgacccgacg acgttcctac cgatcgtgca agcggtgttc ggcgcatcgg cgacccggac
     1861 ccgaagccac accgccgacg ccaccccgtt gatccgctac cgcatcagca ccggctccgc
     1921 cacaagcaac ccggccgtga cggcgttgct cgcgatgttg aatgtcgcca cgggcaggtt
     1981 cgaggtcacc gaagttctcg cactgttgca gatgcccgca gttcgcgatc ggttcgggtg
     2041 gaccgacgac catatcgaac gcatctccgg gtggctcgcc gacaccaacg tccactgggg
     2101 gctcgacccc gcgcatcgcg agcggttcgg ccttcccgcc gccatcaccg gttactcctg
     2161 gcaagaagcg ctcgatcagt tgctcatggg cgcactcgtg ctcggcgacg acgagttggc
     2221 gatcggcgac gtcgttccct atgaccctgc gagttccgag atcgacgtga tcggacaact
     2281 cggttcggtg ctgtcgaccc tcgaacgcct taccgaggct gcgaccacgc cccgcacgat
     2341 cgacgagtgg tgtgcgctcc tctccgacgt catcggcgag accctcggag atacctcacc
     2401 caatggttgg cacctcgacg gtgcgtaccg cctgttggcg cggctcgcat ccacctcgcg
     2461 cctcgcccga gtcggcggcg agcggcgagc cgacggcacc cccgactcct ccaccgcact
     2521 gttgaccctc gccgaggtgc acgcactggc gagcgacacg ctcggcgaat cgcgatctcg
     2581 ctccgatttc tttcggggcg gcatcacggt cacttccctg ggggcgttgc ggaacgtccc
     2641 cagtcgcgtg acctgcgttg tgggcttcga tcaagccgcg ttcggctccg gggcgaccga
     2701 cagcgacgat ctcatcgccg ccacgccacg catcggcgat cgcgatcgac gggccgaggt
     2761 ccgccagaca ctcctcgacg cggtgatgtc gacctcggat cacttcttga cgttcagcga
     2821 cgggcgcgac gtgcagacga acgcggagat tccgcctccg gtgctgctcg ccgaactcat
     2881 cgagacgctg tgcgcaacgc tcggcgtcga cgacgccgag cagcttccgg ccgggatcct
     2941 ggtgcgacat ccacgacacg cattcgacgc tgcgtacttc actgatgatg ccggtgggct
     3001 tcgatcgttc aaccgcagcg cactgcgtgc agcgctggca cgaaggtcgc gtgatccgaa
     3061 cgccacgctc gaccccgact ggccgatgat tcgccttacg gaaccgatga atccgggagg
     3121 cgctgctctc gaaatcgaca cccttcgcaa cgtgatgcgc tcacccaccc gtatgtttct
     3181 ggaaggcggg gttggcattc gccttccccg ggcctccgag cagttgtccg cacgacttcc
     3241 gatcgagttc gatgacctcc agaaatggga actcggtacc gaggtgctcg acatcgagcg
     3301 caccggccag aacctccatc gctggagcga acaccaacgc cgcaagggag tcctcccacc
     3361 gctcggattg gccgaccaga tcatcggcga cctgaaaacc gaggtcacct ccatcattga
     3421 agaagcccaa tcgcggggcc tcgcgctcgt cgagggtgca accgtcggca tcgacctcac
     3481 catcgcgggt gatcgacgca tcctcggttc gatcgtggag gtgttgagcg cgccgcggcg
     3541 cggtaccgcc cacgtgcact ttcgacggcc caagtcggcc cacctcttgg aagcctgggt
     3601 cgatctcctg tgtctcaccg cggcagatcc agccgtaccg tgggaggttc acctcgcgtc
     3661 gagggcgccg tcgagcacgt cgaagccggg cccaccgccc gtcgccatgg tgctgcgagg
     3721 ccgagacctc ggcgccgtaa cggcggtgga ccatctcgag tttctcgtcg ggctccacga
     3781 caccgccatt cgccagccgg tgccgttctt cccagagctg tctcgatcga tcacccaggg
     3841 tgaagaccta ggcactcaga ccaaggtctg gaacgaggag cgcggtcggc tccgcgacga
     3901 acgcctcgtc ttcggcgacc acacgctcag acaactccgc gagttcaccg ccgaaggcca
     3961 cgacatcgtg gcgaactgcg atggcgaaac gctcctcgac cgctacgcca acgcggtgtg
     4021 gagccgcttc gatcaatcgg tctccgtcga gtcagatccc gctgccaacg acgccgacgc
     4081 ctcgccgccg gcaaccgacg acgagccccc gcactctgga ggccagaagc gatgaacgag
     4141 ctgaactccg accctcacgg cgataccgga gaggtcttcg acacctcggc gaagcttccg
     4201 accggtcgtg tctgcatcga agcctccgcc ggcaccggca agaccttcac cctggccgca
     4261 ctggcggtgc ggtcactcgc cgagaccgac gtcccggcag ccgggctcct gatcgtcacg
     4321 ttcacacgcg ccgcaaccgc tgagctgcgc ggtcgtatcc gcgacgccat caacgaggcg
     4381 atcacacacc tcgaagcggc gatcgccgcc ctcgataccg atcagcccgc accgcaaacc
     4441 gacgttgcac acctgagtgc catcgccaag gggacactcc gggagttggt cgagcgctcc
     4501 cgacggctgc gtgtcgctct caccgaatac gacacgatca ccatcaccac cattcacgcg
     4561 ttcgctcaac agacgctcgc gagtctcggc gtcagtagcg gcaccgattc cctcgtcatc
     4621 agcgaggccg atagcaacga cgccgtcatt gacgcatgcg ccgacgttgt cgcccgggcg
     4681 atcggcgaag gccacaaccc ggccatgatg ccgacgttgt ccaaactcgc cgactggacg
     4741 aagttggcgc ttcgctccag cgacctgatc ctggagccgg cgctgaccga caccgacgcg
     4801 gacgatgccg atcagatgcg gactcgtctc gtcaccgaag cggtcgcgtt gctcgcgaga
     4861 cgccgccacg accgtgggct ggcgagtttc gacgatgtgc tcgtggccct gcgcgacgaa
     4921 cttcgacacg cgacacacgc ccccgaacga tcggcgatca tcgacacgct gcgctcgcga
     4981 tatcgcatcg cgctcatcga cgagttccaa gacaccgatc ccgttcagtg ggaaatcttc
     5041 gaaacgatct tcgacggcga cgaatccacc ctcgtgctcg tcggcgatcc gaagcaggcc
     5101 atctacagct tccgcggcgc cgacatcgcg acctattcaa gggcaatcac ggccccggcc
     5161 acccgacgca cgcgcctgcg aaccaactgg cgcgccgacg ggatcgtgat cgacgcactc
     5221 gatgaactgc tgcgcggcgc cacgttcggc gacggaatcg acttcgtcca tgtcgatccg
     5281 gccgagcaca acgtgtcgcg gagcgcccgc cgaaacgacg gtgccacgtt gtctccggtg
     5341 cggattcggc tcgccatcaa cgacgacatc gaacgcacag ggaaaaccgg aacaacccaa
     5401 acggacgcgg tgcgcctggc gatcgccaac gatctggtgg ctcaggtcca cgacgccctc
     5461 gatcacctcg tcattcccga ccaggacggt gaccgagcgg tccagcccga tgatctcgcg
     5521 gtattggttt cgaccaaggc tgaggcggtg gccatgcagc aggcgctcgt cgcgtcgggg
     5581 gtgcctgccg tgctcagccg ggccgaagac gtcctcgaat ccccctcggc cattcaatgg
     5641 cgttggctgc tcgacgccct cacacgccca tccgatccca tgcgcacgcg ccgtttcgtg
     5701 ctcggatggt tcagccaacg cgacatcacc gatctcaccg ccgacccgga tgcggtgctc
     5761 gatgaggccg ccgaacggtt ggcgacgtgg aacgaggtgc tccgcgaccg aggggtcgcc
     5821 gagttcgtgc gacgtatctt cgacgacagt cgggttctgg agcgggtact ggccggcgcc
     5881 gaaggcgatc gagccgcgac cgatctccaa cacctcggcg aactgttcca gacccagtcc
     5941 acccaacgac ccggagtcaa cgagctcctc gccctgctcg acccctcagg tctcgacacc
     6001 gaacacggtg aagtgggcgt cgcgtcgcgg cgcgtcgaga ctcaacagcc ctcggtgcag
     6061 atcatgacgg tctgggtcgc caagggcctc gagtatcccg tcgtgttcgt cccgaccttg
     6121 tactccacgc ccacgaaggt tgatgcgata tgcaccaagc cggacgggac gcgtcgcctc
     6181 ggcctatccg aggacctcag gaagcgctat tcggcggatg ccagacttca ccgcgacaca
     6241 gagcggctcc ggctgttgta tgtggcgatg acccgcggcc gccatcactg cgtgttgtgg
     6301 tggtcgccta tccaccagaa ttcgcgtgcc cctataaccc ggttgtt
//
LOCUS       DAOOMA010000070         6323 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_14674_length_6323_cov_3.803765, whole genome shotgun sequence.
ACCESSION   DAOOMA010000070 DAOOMA010000000
VERSION     DAOOMA010000070.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 6323)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6323)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6323
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14674_length_6323_cov_3.803765"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..358
                     /locus_tag="PLP95_03170"
     CDS             <1..358
                     /locus_tag="PLP95_03170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB44832.1"
                     /translation="MRAECKYFESRSYPTGETVRKCDLDLAPEAPWRCPENCSSFEPR
                     LADVNWAHGSLITPETPAEPASLGNDSSIGDLLDAAEEIVNAVAQQTRAEVDAERARA
                     ARGGLRSKLPKWFRKG"
     gene            481..1737
                     /gene="sufD"
                     /locus_tag="PLP95_03175"
     CDS             481..1737
                     /gene="sufD"
                     /locus_tag="PLP95_03175"
                     /inference="COORDINATES: protein motif:HMM:TIGR01981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HPB44833.1"
                     /translation="MPADALTPTFAESIPGPAWLSAQRLAAAQRYERGEAPTAEREEW
                     RYSRIDDVDVSKYALHDTPTEVAVSSVDLGPFAGRALLRNGQVVAVAIDDALAAKGVV
                     VASARTLTEPLGFDLPEPVGGPDSFDDLNAGFAPDPLVIIVPRGVVVDAPIHIEHHVD
                     AADALIVPRTIVSVGENASLRLVEWVGSEAVDALVVDRTVLHLDNAARVTRVSVNNLA
                     TSVTHLAGVCTSVGQQAELTMDHVALGGAYARNRFDCRLVGRGASGRISALYLGAGDQ
                     MHDLRTFQSHEARDTMSDLVFKGAVDDRAHAVYTGLIQVGEEAAGTNANQSNRVITLS
                     EHAWAESVPNLEIHHNDVKCSHATAVGPIDVEQRFYLESRGVPPEAAERLVVNGFFAE
                     VLGSIADERVVDLVAPLIADKLAGSR"
     gene            1734..2072
                     /locus_tag="PLP95_03180"
     CDS             1734..2072
                     /locus_tag="PLP95_03180"
                     /inference="COORDINATES: protein motif:HMM:NF025178.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme iron oxygenase ferredoxin subunit"
                     /protein_id="HPB44834.1"
                     /translation="MIDGSPGETTVVRLCRLDEIDDGGMARFELDGFNLAVIRIEDKV
                     YGLEDRCSHQNVPLSTGEVDTDECLVVCTKHGAAFDVETGEALILPATRPVPTYDLAV
                     EDGEVLLVLS"
     gene            2086..2883
                     /gene="sufC"
                     /locus_tag="PLP95_03185"
     CDS             2086..2883
                     /gene="sufC"
                     /locus_tag="PLP95_03185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002514361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HPB44835.1"
                     /translation="MSELIIRDLTASVAGKQILNGVNLTVRSGEVHAIMGPNGSGKST
                     LSNVLMGRPGYEVTGGTITLDGVDLIAIPTHERAAAGLFLGMQYPTEVPGVSLTDVLS
                     ESIAIDRSGADLEATLAAEAERVGLATALLDRPLNVDLSGGEKKRNETVQLALLRPKF
                     AVLDEIDSGLDVDALAQVSQRVEDATKEWGLGVIAVTHYNRLLEKLHADVVHIFARGQ
                     ILESGGPELAERLEETGYGHWLDSLPDDEPLSSPDDVIDPFADPLGL"
     gene            2930..3586
                     /locus_tag="PLP95_03190"
     CDS             2930..3586
                     /locus_tag="PLP95_03190"
                     /inference="COORDINATES: protein motif:HMM:NF016300.2"
                     /note="The SIMPL domain is named for its presence in mouse
                     protein SIMPL (signalling molecule that associates with
                     mouse pelle-like kinase). Bacterial member BP26, from
                     Brucella, was shown to assemble into a channel-like
                     structure, while YggE from E. coli has been associated
                     with resistance to oxidative stress; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HPB44836.1"
                     /translation="MIDSNQFDGPASGIQVIGRGRASSAPDVVTLTLGVSVREADAPT
                     AIAAAATAARALLDALGGHGVDERDISTDTYSIQQEWSHVDERSILDGYRVRNIVTCL
                     VRDAGAVGSVLDAATSAAGGAVEVQGLAFAKEDLTDDRDAARQAAVEDARHRGEHLAA
                     LTGRRIGAVEWVRELDVVRRPGEPMVRMAMADSGPATPLMGGEHSTEVVVEVRYSFGD
                     "
     gene            complement(3568..4020)
                     /locus_tag="PLP95_03195"
     CDS             complement(3568..4020)
                     /locus_tag="PLP95_03195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB44837.1"
                     /translation="MAQVRSAILCDFAQVREGLVFISSGGITRIAAPQPGSPVQFFAA
                     CDLEVLPFEIGEAHTLTFKVVNATTAKVAWEANLQVNTTANPDGLFPGESLHVPIAFR
                     VGPFPVEAFGPHDLKVSIDNYETELLTFYVLQMVQPPGQAAEDQSPNE"
     gene            4040..4393
                     /locus_tag="PLP95_03200"
     CDS             4040..4393
                     /locus_tag="PLP95_03200"
                     /EC_number="4.2.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008124 -
                     4-alpha-hydroxytetrahydrobiopterin dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006729 - tetrahydrobiopterin biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4a-hydroxytetrahydrobiopterin dehydratase"
                     /protein_id="HPB44838.1"
                     /translation="MVTDSAPAPRHDGTVTAETVTPDGWQRDSGALVRRFEFVDFASA
                     FAFMTVVAALAEELDHHPDWCNSYNVVTIELRSHDVGAITERDVRLAARINGLLGGVD
                     AGLALDGLVEGDGMP"
     gene            4390..6180
                     /locus_tag="PLP95_03205"
     CDS             4390..6180
                     /locus_tag="PLP95_03205"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HPB44839.1"
                     /translation="MTRSWKYLPALDGIRAFAVAAVVVFHFWPSVLPGGFIGVDIFFV
                     MSGFLITSMMNVEHERHDRIDLAAFWGRRIRRLVPAVLVLVTVCSVALWWTDTGSALY
                     IAHAGGALTWTTNLVEAFFGRSPVFLHNTNTVLDHLWSLAIEEQFYLVWPIAGLLLVR
                     KVASPSRRVMIVGVAIAASATSMAFAGPVVGYFRTDARAFELLAGAALAYSGVTQRPL
                     DARWCGAAAAAGFIVLGAVSVLVEPQDAWLYPWGFFVITAAATLLVAAATNPPPWLVG
                     VLERRAVRHVGQVSYGIYLWHIPVFRILSSGRTGIDSGWLLAAVRLAVLAAVVEASYR
                     FVEQPFRTGRVRFGWGAAGVGYAIALASLLLMVPTAREDWNRRWDSPVEMPTAAQGQR
                     RVLVTGDLSAIVLGDRLAGEPDDSDDDPGSAVAVWTIADAGCSMASGPDDNREVLRGE
                     ESERASGYCRNWPTRWTHAAAAFHPDVAVVSAGFNDTSPMVDGDQRLSPEAAEAVYVE
                     ATRAGIASLRSGHAGIERVYLVTVEDWSALALAGGVWSPERVELQRRYNDALATVADS
                     DPGVSVVTLPADDWSAFGDGAEGLGGRLWR"
BASE COUNT         1018 a         1990 c         2205 g         1110 t
ORIGIN      
        1 catgcgagcc gagtgcaagt acttcgaaag tcgctcctat ccgaccggtg aaaccgtgcg
       61 gaagtgcgac ctcgacctcg ccccggaggc gccgtggcgg tgtccggaga actgttcgtc
      121 cttcgagccg cgcctcgccg atgtcaactg ggcccacggt tcgctcatca cccccgaaac
      181 ccccgcggaa cccgcaagtc tgggcaacga ttcgtcgatc ggcgatctgt tggatgcggc
      241 cgaagagatc gtgaacgccg tggcgcaaca gacgcgggcc gaggtcgatg cggaacgtgc
      301 ccgagcggcc cgcggcgggc tgcgttcgaa gctgccgaag tggtttcgca agggttgagc
      361 tgacacgtct tggacgaagt cgtcgctttt cggcggcagg gttccctcag ctaggatttc
      421 tcctatcgcc atgggactaa ttcccgatcg gcatagatcg aacgaggacg caacgacgcc
      481 gtgcctgccg acgcactcac cccaaccttc gccgaatcca ttcccggacc cgcttggctc
      541 agcgcacagc gcctcgccgc tgcacagcgt tacgaacgtg gcgaagcgcc gaccgccgaa
      601 cgtgaagagt ggcgctacag caggatcgac gacgtcgacg tctcgaaata tgcactgcac
      661 gacacgccga ccgaggtggc ggtctcatcg gtcgacctcg gcccgttcgc cggacgcgca
      721 ctgctgcgca acggccaggt cgtggccgtt gcgatcgacg atgcgttggc cgccaaaggc
      781 gtcgtcgtgg cgtccgcccg cacgctgacc gaaccgctcg ggttcgacct gcccgaaccc
      841 gtcggtggac cggacagctt cgacgacctg aacgccggct tcgctcccga cccgctggtg
      901 atcatcgtgc cacgcggtgt cgtggtcgat gcgccgatcc atatcgagca ccacgtcgat
      961 gccgccgatg ccttgatcgt gccgcggacc atcgtgtccg tcggcgagaa cgcgtcgctg
     1021 cggctcgtcg agtgggttgg ctccgaggcc gtcgacgcgc tcgtggtcga ccgcaccgtg
     1081 ttgcacctcg acaatgcggc gagggtcacc cgcgtgtcgg tcaacaacct cgcgacctcg
     1141 gtgacccacc ttgctggggt gtgcacgtcg gtcgggcagc aggccgaact cacgatggac
     1201 cacgtcgcac tcggcggggc ctacgcccgg aaccgtttcg actgtcgact ggtcggccgc
     1261 ggtgcatccg ggcgcatctc ggcgctgtat ctcggtgcgg gcgaccagat gcacgacctg
     1321 cgcacgttcc agagccacga ggcgcgcgac accatgtcgg acctggtgtt caagggggcg
     1381 gtcgacgatc gggcgcacgc ggtgtacacc ggcctcatcc aggtgggcga ggaagcggcg
     1441 ggcaccaacg ccaaccagtc gaaccgggtg atcacactct cggagcatgc ctgggcggaa
     1501 tcggttccga atctggagat ccaccacaac gatgtgaaat gcagtcacgc gaccgcggtc
     1561 ggcccgatcg atgtcgagca gcgtttctat ctcgaaagcc ggggcgtacc gccggaggcc
     1621 gccgaacggc tggtcgtgaa cggattcttc gccgaggtgc tcggctcgat cgccgatgaa
     1681 cgcgtcgtcg acctggtcgc cccgctgatc gccgacaagc tggcgggttc gcgatgatcg
     1741 acggttcacc tggtgagacc accgtcgtgc gtttgtgccg gctggacgag atcgacgacg
     1801 gcgggatggc tcgctttgaa ctcgacgggt tcaacctggc cgtgatccgg atcgaggaca
     1861 aggtctacgg gctcgaagac cggtgttcgc accagaacgt cccgttgagc accggcgagg
     1921 tcgacaccga cgagtgtctg gtcgtgtgca cgaaacacgg tgcggccttt gacgtcgaaa
     1981 ccggtgaggc gctgatcctg cccgccaccc gtcccgtccc gacctatgac ctcgccgtcg
     2041 aggacggcga ggtgttgctg gtgttgtcat gaacgaggtg ttgtcatgag cgagttgatc
     2101 atccgagacc tgacggcgag tgtcgccgga aagcagatcc tcaacggggt gaacctcacg
     2161 gtgcgcagcg gggaggtgca cgccatcatg gggccgaacg gttcgggaaa gtcgacgctg
     2221 agcaacgtgt tgatgggccg gcccggctat gaggtcaccg gaggaacgat cacgctcgac
     2281 ggggtcgatc tcatagcgat accgacccac gaacgggccg ctgccgggtt gtttctcggg
     2341 atgcaatatc ccaccgaagt gcccggcgtg agcctcaccg acgtgttgtc ggagtcgatc
     2401 gccatcgatc gttccggagc cgacctcgag gcgacactgg ccgccgaggc cgagcgggtc
     2461 ggactagcga cggcgctgtt ggatcgtccg ctcaacgtcg acctgtcggg cggcgagaag
     2521 aagcgcaacg agacggttca gttggcgttg cttcgtccga agttcgcggt actcgacgag
     2581 atcgattcgg gcctcgacgt cgacgcgctc gcgcaggtct cccagcgggt cgaggacgcg
     2641 acgaaggaat ggggcctcgg cgttatcgcg gtgacccact acaaccgatt gctcgagaag
     2701 cttcacgccg atgtcgtgca catcttcgcc agaggacaga tcctcgagtc gggcggcccc
     2761 gaacttgccg aacggcttga agagacgggc tacggccact ggctcgactc gttgcccgac
     2821 gacgagcccc tgtcctcgcc agacgacgtg atcgacccct tcgccgaccc gctgggactc
     2881 tgagcgccgc ctgggacaga ctcggggcca acgcgacgag gagcgagaag tgatcgatag
     2941 caatcagttc gacggcccgg ccagcggaat tcaggtgatc gggcgcggcc gcgcatcgag
     3001 cgcacccgat gtcgtcacgt tgacccttgg ggtttcggtt cgcgaagccg atgccccgac
     3061 cgccatcgcg gcggcggcga cggctgctcg ggcgctcctg gacgcgctgg gcggccacgg
     3121 tgtcgacgaa cgggacatca gcaccgacac ctattccatc cagcaggagt ggagtcacgt
     3181 cgacgaacgt tcgatcctgg acggctaccg ggtgcgcaac atcgtcacct gcctggttcg
     3241 cgatgccggg gccgtgggat cggtgctcga cgcggcgacc tcagcggcag gtggagccgt
     3301 ggaggtgcag ggcctggcgt ttgccaagga agacctgacc gatgaccgcg acgcggcccg
     3361 ccaggccgcg gtcgaagatg cccgtcatcg cggtgagcac ctggcggcgc ttaccggccg
     3421 tcggattggg gccgtggagt gggttcgcga actcgatgtg gtgcgtcgcc ccggcgaacc
     3481 gatggtccgc atggccatgg ccgactccgg ccccgccacc ccgctcatgg gcggtgagca
     3541 cagcaccgag gtcgtcgtcg aggtccgcta ctcgttcgga gactgatcct cggccgcttg
     3601 gcccgggggc tggaccatct gaagcacgta gaacgtcaac agttcggtct cgtagttgtc
     3661 gatcgacact ttgaggtcgt gcggaccgaa cgcctcgacc gggaacgggc cgacgcgaaa
     3721 cgcgatcggc acgtgcaggc tctcgccggg aaagaggccg tcggggttgg ccgtggtgtt
     3781 gacctggagg ttcgcctccc acgcgacctt cgccgtcgtg gcgttgacca ccttgaacgt
     3841 cagggtgtgg gcctcgccga tctcgaatgg caagacctcc aggtcgcacg ctgcgaagaa
     3901 ctgcaccggt gagcccggtt ggggggcggc gatgcgggtg atgccgccgg aggagatgaa
     3961 gacgagaccc tctcggacct gcgcaaagtc gcagaggatt gcggaacgga cttgtgccat
     4021 ggcgccaccc taccggtcga tggtgaccga ctctgctccg gccccgcgcc atgatggaac
     4081 cgtgacggcc gaaacggtga ccccagatgg atggcagcgc gacagcggag cgctcgtgcg
     4141 gcgattcgag ttcgtcgatt tcgcttcggc gttcgccttc atgacggtgg tggccgcgtt
     4201 ggccgaggag ctcgaccatc acccggattg gtgcaactcc tacaacgtgg tgacgatcga
     4261 actgcgcagc catgacgtcg gtgcgatcac cgaacgggac gtgcgccttg ccgcgcgtat
     4321 caacgggctg ttgggtgggg tggatgcagg gctggcgctt gacgggctgg tcgagggcga
     4381 cgggatgccg tgacccgatc ctggaagtat ctccccgcgc tcgacggcat tcgcgcgttc
     4441 gccgttgccg ccgtcgtggt tttccatttc tggccgagcg tgttgcccgg cgggttcatc
     4501 ggggtcgaca tcttcttcgt catgtcaggg ttcctgatca cctcgatgat gaatgtcgaa
     4561 cacgaacgcc acgaccgcat cgacctggcg gcgttctggg gacggcggat ccgcaggctg
     4621 gtgccggcgg tgctggtgct cgtcacggtg tgttcggtcg cgctgtggtg gaccgatacc
     4681 ggatccgcgc tgtacatcgc tcatgcgggc ggagcgctga catggaccac caatctcgtc
     4741 gaggcgttct tcggccgttc gccggtattc ctccacaaca ccaacaccgt gctcgatcac
     4801 ctgtggtcgc tggcgatcga agagcagttc tacctcgtgt ggccaattgc cgggctgctg
     4861 ttggtccgca aggtcgcgtc cccgtcgagg cgtgtgatga tcgtcggcgt cgccatagcg
     4921 gcttcggcga cgtcgatggc gttcgccggt ccggtggtcg gctacttccg caccgatgcc
     4981 cgcgccttcg aactgctcgc cggagcggcc ctggcgtact cgggggtcac acagcgtccg
     5041 ctcgacgccc gttggtgcgg ggcggccgcc gcggccgggt tcatcgtgtt gggagcggtc
     5101 agtgtgttgg tcgaaccaca ggacgcatgg ctctacccat ggggattttt cgtgatcacc
     5161 gccgccgcga ccttgctggt cgccgcggcg accaatccgc caccgtggtt ggtgggagtt
     5221 ctcgagaggc gagcggttcg ccatgtcggg caggtgagct acggcatcta cctgtggcac
     5281 atcccggtgt ttcggatcct gtcgtcgggc cgcaccggaa tcgacagcgg gtggctcctg
     5341 gccgcggtga ggctcgccgt gctggcggcc gtggtggagg cgtcgtatcg gttcgtcgag
     5401 cagccgtttc gaaccggacg tgtgcgattc ggttggggag ccgcgggtgt gggctacgcg
     5461 atcgcgctcg cgtcgttgtt gctgatggtg cccaccgccc gcgaggactg gaaccggcgt
     5521 tgggactccc cggtggagat gccgaccgcg gcccagggtc agcgccgagt gctggtcacc
     5581 ggagatctca gtgcgatcgt cctcggcgat cgacttgccg gcgagcccga cgactccgac
     5641 gatgatccgg gcagcgcggt ggcggtgtgg acgatcgctg atgccgggtg ttcgatggcg
     5701 agcggtccgg acgacaaccg cgaggtactt cgcggcgagg agtccgagcg ggcatcgggg
     5761 tactgccgca actggcccac gcgctggacc catgcagcgg cggcgttcca cccggacgtc
     5821 gcggttgttt cggcggggtt caacgacacg tcgccgatgg tcgacggtga ccagcggctc
     5881 tccccggagg cagccgaggc ggtgtacgtc gaggcgactc gtgccggcat cgcgtcgctt
     5941 cggtcgggcc acgctgggat cgagcgggtc tatctggtca cggtggagga ctggtcggcg
     6001 ttggcgctcg ccggtggtgt gtggagcccg gaacgcgtcg aactacagcg tcggtacaac
     6061 gatgcgctcg ccacggttgc cgacagcgac cccggggtct cggtcgtcac actcccggcc
     6121 gacgattgga gtgcgttcgg agacggcgca gaagggttgg gtggacggct gtggaggtga
     6181 gtggataggc ggaggaacga ggcgtatcag cgtggcccct gagcgccgaa cctatgccga
     6241 ggtgagcccc tcgtcgaggg tgttccacga caacgttgcg cacttgatac gcaccgggaa
     6301 tttcaccact ccgcgaagcg cca
//
LOCUS       DAOOMA010000071         6321 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_14678_length_6321_cov_4.222630, whole genome shotgun sequence.
ACCESSION   DAOOMA010000071 DAOOMA010000000
VERSION     DAOOMA010000071.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 6321)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6321)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6321
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14678_length_6321_cov_4.222630"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..382
                     /locus_tag="PLP95_03210"
     CDS             <1..382
                     /locus_tag="PLP95_03210"
                     /inference="COORDINATES: protein motif:HMM:NF015279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HPB44840.1"
                     /translation="DNDERARRAAVDYRHALHLMAPATPTWTPPVLACSGLTNMGLAD
                     IWEQILVHRAKMSATGELDDRRRTQQVHWMWAMVKDRLLELFRDDPGVAALLVEVEAR
                     VGAGEMPATAAADRLLERFEHRST"
     gene            450..1271
                     /locus_tag="PLP95_03215"
     CDS             450..1271
                     /locus_tag="PLP95_03215"
                     /inference="COORDINATES: protein motif:HMM:NF024758.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HPB44841.1"
                     /translation="MLRRVPPSSRPPFDERTNVAKPLLVIGAAALIGAALGTALGRDA
                     NQGTGVGAQEVSVLGVSIERSEHGEDDSMIGVDVGREAWSDSVVRIRSDRCDARNTVA
                     TGVVIDGVVLTNRHVVEGAATIEVETLDGRRFDAVSMRQSTWIDLAAFSVAELDGGLE
                     LALAQSAQRQVQHPKGPTWTMPGFPEGGALVVESVAAVATRRGVAFPDPPEAVELSHR
                     VVPGQSGSPVIDSDNKVAGILYASAHDSGAGLMIDAATITQAWPQMRRVGFSSCS"
     gene            complement(1255..2223)
                     /gene="gluQRS"
                     /locus_tag="PLP95_03220"
     CDS             complement(1255..2223)
                     /gene="gluQRS"
                     /locus_tag="PLP95_03220"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF004314.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA glutamyl-Q(34) synthetase GluQRS"
                     /protein_id="HPB44842.1"
                     /translation="MSTGRFAPSPTGDLHLGNLRTAVLAWLWARRDRGGFIVRMEDLD
                     RVTSRPEFERSQLADLEALGLDWDGVVVRQSERFDRYHEVLVDLEARGLTFECFCTRR
                     EIAQAASAPNGPATSGAYPGTCRNLSEARRRRLRDEGRPAAIRLRSDHRCTVFVDEVL
                     GERSGCVDDVVLRRNDGVPAYNLAVVIDDVAQGIDLVVRGDDLVDTVPTQALLREVLG
                     AARARFAHVPLVMSERGTRLAKRDGAVTLADRHAIGDSTSAVLWWLLASIGVELDRDL
                     AHASPRELMTSALDLFDRDRLPREALRLTDEFLARPLPDGDATNRN"
     gene            2305..2964
                     /locus_tag="PLP95_03225"
     CDS             2305..2964
                     /locus_tag="PLP95_03225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HPB44843.1"
                     /translation="MPRLKEIRRADAPSPLVETMYGFLFGDRDPVEEPGTTTGTPGNW
                     WTVFAAVPDIFQHSVDGFRVYRSSRRSLDPVLRELGQTRAGWACGSQFVFSQHCKSLR
                     LLGVPESKIEAVAYWPASDEFTDIERAVLGYTDALALQQGRVHDKLFAKLQEHLSDEE
                     ILELTYITGLYIMHAIMSRALHTEWDERVDPIVEVESPDGFAGYDIGATIAGGSDKVE
                     G"
     gene            2961..4205
                     /locus_tag="PLP95_03230"
     CDS             2961..4205
                     /locus_tag="PLP95_03230"
                     /inference="COORDINATES: protein motif:HMM:NF012296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HPB44844.1"
                     /translation="MTSALTPTEADALLSAMITDPEARQDPPATFRRLHAELPAWDSA
                     LGMTVLAGYHDGLETLRSPKWGRAEDDMDLPESFGTAERGDRERIPSMLLMNPPDHTR
                     VRSLVARAFTPRMVERLRPQIEAILDPLLDCLATERECDLMTTLAVPFPVAVISALLG
                     VPPEKGADPSFVQQIRHLTSFIDSGASAEDIAAAQLAAVEVAGYFTELVEVKRADPDD
                     AMLSALIEVEEAGDRLSHEELIINSILMYAAGFETTSNLIGNGMWALLRNPDQLELLC
                     SDRSLMPAAIWEMLRFDSPVQINMRFSLEPVEFLGRTVERGHSAMVLQGAGNHDPAVY
                     DDPDRFDVGRFAAEGVPQPLSFGWGAHHCLGAHLARAEGEILFTRLLDRFGSIELVGE
                     PPRYRAHATLRGLESLHVRLAE"
     gene            complement(4286..4362)
                     /locus_tag="PLP95_03235"
     tRNA            complement(4286..4362)
                     /locus_tag="PLP95_03235"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4326..4328),aa:Pro,seq:cgg)
     gene            complement(4439..4843)
                     /locus_tag="PLP95_03240"
     CDS             complement(4439..4843)
                     /locus_tag="PLP95_03240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB44845.1"
                     /translation="MQRVLLATDSDELFAEIDSAIGSNDVAVVRVRAGVDVRPAIQEV
                     QPDIVLLDLQIGSMGAYAVTYDLRNEEGAGRIDPQNIVLLLDRDVDTFLAERSGADGW
                     LVKPLTPRRIQRGVDTVLRGEVFEEGSALFEV"
     gene            4968..5339
                     /locus_tag="PLP95_03245"
     CDS             4968..5339
                     /locus_tag="PLP95_03245"
                     /inference="COORDINATES: protein motif:HMM:TIGR00377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HPB44846.1"
                     /translation="MAGNRKEQTVVEVMIVREEAATVCRPAGELDAYSVQAFRDHLAE
                     LAGEPSVVIDLSEVPFMDSAGLGALIGGIRKIREAEGEVVVVCDRAAVLRLLHTTRFD
                     RMVSVVASCDDAYAEFNSTLD"
     gene            complement(5352..>6321)
                     /locus_tag="PLP95_03250"
     CDS             complement(5352..>6321)
                     /locus_tag="PLP95_03250"
                     /inference="COORDINATES: protein motif:HMM:NF014433.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ArsA-related P-loop ATPase"
                     /protein_id="HPB44847.1"
                     /translation="GRRAVVVTIDPAKRLADALGLDELTNEPKPIDGPWIGTLAALML
                     DTESTFNNLIARYAGSAEQAEKILSNRFYRNIAGALSGTQEYMATEKLYELAASGDFD
                     LIVVDTPPSRNALDFLDAPGRIVRFLDHPAYRMMTAPGRGIVKAVNYAAQPILRGLGK
                     AIGGEVIADAIAFFAAFDGMEEGFKERARLVDRLLGEERTEFVLVASPRRDTVIEASF
                     FANKLASMKQAVAAVVVNRMYPTFEPVADDVRTAAEAAGDGALRAHLRNLDELDALGR
                     AERLELAPLTMQVEPAPIIEVPLLSTDVHDLDSLALLADHMFGRAD"
BASE COUNT         1081 a         1979 c         2126 g         1135 t
ORIGIN      
        1 agacaacgac gagcgggccc gcagagcggc cgtcgactat cgccacgccc tgcacctgat
       61 ggcgccggcg acgccgacgt ggaccccgcc ggtgctcgcc tgttccgggc tgacgaacat
      121 gggtctggcc gacatctggg aacagatcct ggtgcaccgg gccaagatgt cggcgaccgg
      181 cgaactcgac gaccgtcggc gaacccagca ggtccactgg atgtgggcga tggtgaagga
      241 tcgtcttctc gaactctttc gtgacgatcc gggggtggcc gcgctgctcg tcgaggtcga
      301 ggcgcgggtg ggtgcaggcg agatgccggc aaccgccgcc gccgatcggc tacttgaacg
      361 attcgagcac cgatcaacct gagcgcactc ccgaatgtga tgtagtgaaa ggccttgtca
      421 ttcgttgaga aatctggtca tctcacggag tgcttcgccg tgttccccct tcgtctcgcc
      481 cgcctttcga tgagcgcacc aacgttgcga agccgctcct ggtcatcggt gcggcggcac
      541 tgatcggagc ggcgctcgga accgcactgg gccgagacgc gaatcagggc accggagtcg
      601 gcgctcagga ggtgtcggtg ctgggggtca gtatcgaacg ctctgaacac ggcgaggatg
      661 attcgatgat cggcgtcgac gtcggccgcg aagcgtggag tgactcggtg gttcgcatcc
      721 gcagcgatcg ttgcgatgct cgcaatacgg tggccaccgg cgtggtgatc gacggggtgg
      781 tcctcaccaa tcgccacgtc gtggagggtg cggcgaccat cgaggtggaa acgttggatg
      841 gccgccgctt cgatgcggtg tcgatgcggc aatcgacctg gatcgatctc gcggcgttca
      901 gcgtcgccga actcgacggg ggattggagt tggcgctggc tcagtctgcg cagcggcagg
      961 tgcagcaccc gaaggggccg acgtggacga tgccggggtt ccccgaagga ggcgcactcg
     1021 tcgtcgagtc cgtcgccgcg gtcgcaacgc gacgcggcgt ggcatttccg gatcctcccg
     1081 aggcggttga actcagccat cgcgtggtcc ccggtcagtc gggaagcccc gtcatcgact
     1141 cggacaacaa agtcgcgggg atcttgtacg cgagcgccca tgacagtggg gccggactga
     1201 tgatcgacgc cgcgaccatc actcaagcct ggccacagat gcggcgcgtc gggttcagtt
     1261 cctgttcgta gcgtccccgt cgggtagcgg gcgggccaag aactcgtccg taagtcgcag
     1321 tgcctcacgg ggtaatcgat cgcggtcgaa caggtcgagt gccgaggtca tcagttcacg
     1381 gggtgacgcg tgtgccaggt cgcggtcgag ttctacaccg atcgatgcca gaagccacca
     1441 cagcaccgca ctcgtggagt ctccgatcgc gtggcgatcg gccagtgtga ccgccccgtc
     1501 tcgctttgcg agccgcgtac ctcgctcgga catcacgagt ggcacatgcg cgaagcgcgc
     1561 gcgagccgcc ccgagcacct ctcgcaagag cgcctgagtt gggaccgtgt cgacgaggtc
     1621 gtcgccccgt acgacgaggt cgatgccctg agcgacgtcg tcgatgacca cggcgaggtt
     1681 gtaggcgggc actccgtcgt tgcgtcgcag cacgacgtcg tccacgcatc cgctgcgctc
     1741 gccgagcacc tcgtcgacga agaccgtgca tcggtggtcg cttcgcagcc tgatcgccgc
     1801 ggggcgaccc tcgtcgcgga gccgtcgacg ccgggcctcg ctgaggtttc gacaggtgcc
     1861 tgggtaggcg ccgctggtcg cggggccatt cggcgcgctt gcggcctgcg cgatctctcg
     1921 acgcgtgcag aagcactcga aggtcaatcc gcgtgcctcg aggtcgacca gcacctcgtg
     1981 atagcgatcg aatcgttccg actgacgaac gaccacaccg tcccagtcga ggccgagggc
     2041 ctcgaggtcg gcgagttgcg accgttcgaa ctcgggccgc gaggtcacgc ggtcgagatc
     2101 ctccatccgc acgatgaagc caccgcggtc gcgtcgggcc cacagccatg ccagcacggc
     2161 ggttcggagg ttgccgaggt ggaggtcgcc ggtcgggctc ggtgcgaatc tcccagtact
     2221 cacggtggcc atcatgacat cgccgaccgg aatcaaccat cgcttggcgg cgcaccatcc
     2281 ggcgcatcgc cgtagtgtgc ccacatgcct cgcctgaaag aaatccgccg agccgacgca
     2341 ccctcgccgc tcgtcgaaac gatgtacggg ttcctgttcg gagaccgtga tcccgtggaa
     2401 gagcccggaa cgaccaccgg gacccccgga aactggtgga cggtctttgc cgccgtaccg
     2461 gacatcttcc agcattcggt cgacgggttc cgggtgtatc gcagctcgcg ccgttcactc
     2521 gacccggtgt tgcgtgaact cggccagacc cgcgccggat gggcgtgtgg gagccagttc
     2581 gtgttcagtc agcactgcaa gtcgcttcgc ttgctcggcg tgccggaatc caagatcgag
     2641 gcggtggcgt actggccggc gtcggacgag ttcaccgaca tcgagcgtgc ggtgctcggc
     2701 tacaccgacg cgctcgcact gcagcagggt cgggtgcacg acaagttgtt cgccaagctg
     2761 caagagcacc tcagtgacga ggagatcctc gaactgacct acatcacggg gctctacatc
     2821 atgcacgcca tcatgagtcg cgcgttgcac accgagtggg acgagcgggt cgatccgatc
     2881 gtcgaggtgg agagccccga cgggttcgcc ggctacgaca tcggcgccac catcgcgggc
     2941 ggcagcgaca aggtggaggg atgacctccg cgctgacccc gacggaggcc gatgcgctcc
     3001 tcagcgcgat gatcaccgac ccggaggctc gccaagaccc tccggccacc ttccgacgcc
     3061 ttcatgcgga gcttccggcg tgggacagcg ccttgggtat gacggtgctc gcgggctacc
     3121 acgacggact tgagacgttg cgaagcccga agtggggccg ggccgaggac gacatggatc
     3181 tgcccgagtc cttcggtacg gccgaacggg gcgatcgcga gcggattccg tcgatgctgc
     3241 tcatgaatcc gcccgatcac actcgggttc gttcactcgt ggcccgagcg ttcaccccga
     3301 ggatggtcga acgacttcga ccgcagatcg aagccattct cgacccgctc ctcgattgtt
     3361 tggccaccga acgcgagtgt gacctgatga cgacactcgc ggtgccgttt ccggtagcgg
     3421 tgatctcggc gctgctcggt gtcccgccgg agaagggggc agatccgtcg ttcgtccaac
     3481 agattcgcca cctgacgtcg ttcatcgatt ctggggcgag tgcggaggac atcgccgcag
     3541 cccagttggc cgcggtcgag gtggccggat acttcaccga actggtcgag gtgaagcggg
     3601 ccgaccccga cgacgcgatg ctgagcgcgt tgatcgaggt ggaggaagcg ggtgatcgac
     3661 tcagccatga ggagctcatc atcaactcga tcctcatgta cgcggccggg ttcgagacga
     3721 cctcgaacct catcggcaac gggatgtggg cgttgctgcg caacccggat caactcgagc
     3781 tgctgtgttc ggatcggtcg ctcatgccgg cggcgatctg ggagatgctg cgcttcgaca
     3841 gtccggttca aatcaacatg cgtttctcgc tcgagccggt ggaattcctt ggccggacgg
     3901 tggagcgggg gcactctgcg atggtgttgc agggggcggg gaaccacgac ccggcggtct
     3961 acgacgatcc cgatcgcttc gacgtgggtc gattcgccgc cgagggtgtc ccgcaaccat
     4021 tgagtttcgg atggggcgcc caccactgtc tgggggctca cctcgcccga gctgaaggtg
     4081 agatcctgtt cactcggttg ctggaccggt tcggttcgat cgagttggtc ggcgagccgc
     4141 cgcggtatcg ggcccatgcc acgcttcgtg gcctcgaatc gctccacgtt cgcctcgcgg
     4201 aataacgaaa gaacctgttc tccgataacc agtggtcgtg atcacgacca ctggttatcg
     4261 gagaacaggc ctaacgagag tcttttggtc gggttgagag gatttgaacc tccgacctct
     4321 gcgtcccgaa cgcagcgctc taccaagctg agccacaacc cgatgcggta gggcagtgta
     4381 ccccgtcggc cggtcgagta cgaaccgcgt tcgaccgccg cgaaccgtgt ctctgtggtc
     4441 acacctcgaa gagggcggag ccttcttcga acacctcgcc gcgcaacacc gtgtcgaccc
     4501 cacgctgaat ccgacgtggc gtgagtggct tgaccagcca gccgtcggcc cccgaccgtt
     4561 cggcaaggaa cgtgtcgacg tcgcgatcca acaacagcac gatgttctgc gggtcgatgc
     4621 gaccggcacc ttcctcgttg cgcaggtcgt aggtgacggc gtaggcaccc atcgatccga
     4681 tctgcaggtc cagcaacacg atgtcgggct gcacttcctg aatcgctggg cggacgtcga
     4741 ccccggcgcg gacacgcacg acggcgacat cgttcgatcc gattgcggaa tcgatctccg
     4801 cgaacagttc atcggagtcg gtggctaaca agactcgttg cacgcacgtc agggtagttc
     4861 cctggccggg taactcccaa cctgatggtg ctcgcggggt gaacggattc tttcgggcgg
     4921 gcgcggtgtc gacatcgcgg atcggaccac taccatcgtg gaaccacatg gccggcaacc
     4981 ggaaggagca gaccgtggtc gaggtgatga tcgttcgaga agaggcggcg acggtgtgtc
     5041 gtccggcagg tgaactcgac gcgtacagcg tccaggcttt tcgagaccat ctggccgaac
     5101 tcgccggcga accctcggtg gtgatcgacc tgtcggaggt tccgttcatg gattccgccg
     5161 ggctcggagc gctgatcggc ggtatccgca agatccgtga ggctgaaggc gaggtggtgg
     5221 tggtgtgcga tcgcgccgcg gtgttgcgac tccttcacac cactcgattc gatcgtatgg
     5281 tgagcgtcgt ggcctcctgc gacgacgcct acgccgagtt caactcgacc ttggactaga
     5341 cggcacttcg gctagtcggc tcgcccgaac atgtggtcgg cgagcagcgc gagtgagtcg
     5401 aggtcgtgta cgtcagtgct caagagcggc acttcgatga tcggcgccgg ctcgacctgc
     5461 atcgtgagcg gtgccagttc aagtcgttcg gcgcgcccca gggcgtcgag ttcgtcgagg
     5521 ttgcgcaggt gggcccgcaa cgctccatcg ccagcggcct cggccgcggt tcgtacgtca
     5581 tcggccactg gctcgaaggt tgggtacatg cggttgacca ccactgcagc caccgcctgt
     5641 ttcatggagg ccagcttgtt ggcgaagaag ctcgcctcga tgacggtatc gcgacgaggt
     5701 gaggcgacga gcacgaactc ggtgcgctcc tcgccgagca aacgatcgac gagacgagcg
     5761 cgttctttga acccctcctc catcccgtcg aacgctgcga aaaaggcgat ggcatcggcg
     5821 atgacctcgc caccgatggc ctttccgagt ccgcgcaaga tcggctgagc ggcgtaattg
     5881 accgctttga cgatgccgcg cccgggggcg gtcatcatcc gatatgccgg atgatcgagg
     5941 aaccgcacga ttcgaccggg tgcgtcgagg aagtcgagcg cgttgcggct cggtggggta
     6001 tcgacgacga tgaggtcgaa gtctccgctg gcggcgagtt cgtagagttt ctcggtcgcc
     6061 atgtattcct gggtgccgct gagcgccccg gcgatgtttc ggtagaagcg gttcgacagg
     6121 atcttctcgg cctgctcggc cgaaccggcg tagcgggcga tgaggttgtt gaacgtcgac
     6181 tcggtgtcca acatcaacgc cgccaacgtc ccgatccagg ggccgtcgat cggcttgggt
     6241 tcgttcgtca actcgtcgag accgagggcg tcggcgagcc gcttcgccgg gtcgatggtc
     6301 accacgacgg cccgacgacc g
//
LOCUS       DAOOMA010000072         6280 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_14802_length_6280_cov_3.790522, whole genome shotgun sequence.
ACCESSION   DAOOMA010000072 DAOOMA010000000
VERSION     DAOOMA010000072.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 6280)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6280)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6280
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14802_length_6280_cov_3.790522"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..711
                     /locus_tag="PLP95_03255"
     CDS             <1..711
                     /locus_tag="PLP95_03255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavin oxidoreductase"
                     /protein_id="HPB44848.1"
                     /translation="RTRFWRSVVEGLRDRAPGLAVAVRLSVFDLVAFRAGADNVGEPV
                     CAVGTPRPFGATDDGLDVDLAEPTRFLELARQLGVRLVCTTAGSPYYNPHIQRPAYFP
                     PSDGYRPPEDPLVGVARQIAATAALRRASPDMTIVGSGYSYLQDWLPEVAEAVVSSGG
                     ADLIGLGRMMLSYPHLAADTLAGAERNRRLICRTFSDCTTAPRSGLVSGCYPLDDFYN
                     ERPERIELAAIKRRLREP"
     gene            868..1383
                     /locus_tag="PLP95_03260"
     CDS             868..1383
                     /locus_tag="PLP95_03260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB44849.1"
                     /translation="MRLTPLMTAAAVGALLTAAACTPSTGGGSSTTAPPTTPTTEAPQ
                     PLEPFEVSGTADKTVGCFSGLLGDDNQEVNLGSDGWWNFGFQGCSLGATETVALLGVA
                     PHVADGFKAPTGYECKLAIAGTSDETPWYTNQIGSTADTGGKVFLSSSSAWPNTTTWT
                     ITCRPLTPIPM"
     gene            complement(1472..2413)
                     /locus_tag="PLP95_03265"
     CDS             complement(1472..2413)
                     /locus_tag="PLP95_03265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF808 domain-containing protein"
                     /protein_id="HPB44850.1"
                     /translation="MAGGLVALLDDIAALAKLAAATLDDASAAALKASSKTVGIVVDD
                     AAVTPTYVQGLAAERELPIIKKIAAGSLRNKLVFILPAALLLSAVAPKLIEVILIFGG
                     GYLAYEGAHKIHARITGHGHDHDDDLPAAMSGEEAEKKTISSAVRTDLILSTEIMVLA
                     LKEVIDDPFINRLLVLVIVAFLMTAIVYGAVALIVKMDDVGLRMATVDNGRSERIGRA
                     LVAAMPKVLSTLTVVGTAAMLWVGGHILLSSAAALGWHGPYDLVHRIEQPFHDVSGVG
                     GVLGWVANTLCSAIVGLMVGLALLFAGGLVQRKRGAH"
     gene            2502..3836
                     /locus_tag="PLP95_03270"
     CDS             2502..3836
                     /locus_tag="PLP95_03270"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygenase MpaB family protein"
                     /protein_id="HPB44851.1"
                     /translation="MGVALEKLRDRVERQGIELPGMYGAFDFNAIPERLALRPEDETE
                     LDTVPRVNRADFLADQGLMDLMAAATMLGDAVCDAYVALIPTHGMHGLITMLRTACQG
                     RIDDVEDPPDELRRFITELETVPAWIDLELVAEGARLERISAALLAPFGVRGAFLATF
                     LNSYAALPMAVTGSLSDKRSAKRVNETAAFFASTVLPGGMDRNGPGFEAAAMVRLMHS
                     MVRYNALRPERNWDQSVYGVPIPQVDQMPAGLITSFLMAVEAVHAKRGYTDDERAMVE
                     MARYRCYLLGLPEVLLPDDPEGLMRVFLARAATLRRGYDDSTCGELVRGTLAAYLRPD
                     TTLKSRVAESFERSFSTAFFIRAFLGNDANRAAEMGVSLTGADKVRVALGAPFIFGRH
                     RIVSRLNGVARLQPAIDRYIVRRLNRRLRDYGHAEYRSDAASYTDRRALVGR"
     gene            3886..4920
                     /locus_tag="PLP95_03275"
     CDS             3886..4920
                     /locus_tag="PLP95_03275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HPB44852.1"
                     /translation="MTKTNPGNFFEDFTLGQVIDHAVPRTITEGDRALYTSLYPTRFA
                     LPSAATFAAGVGLAAPPVEELVGFHVAFGKTVPDVSLNAVANLGYAELRFHRPVLVGD
                     TLRSSSEVIGLKENSNGKTGVVYVRSTATNQRGEVAIDWARWVMVNKRDHSAAAPETV
                     VPELAKAVSPEDLLIPDGLDFTRYDFSAAGEPHRFDDYEVGEKIDHVDGVTLTDPEHM
                     LATRLWQNTAKVHFNTEARPDGRRLIYGGHIISMARALSYNGLANAQLIAAINAGAHT
                     NPAFADDTVYAWSEVLDKADTSAPGVGAIRLRLVATKGRDPSMTLKGEDGKYASNVLL
                     DLDLWAFMPR"
     gene            complement(4977..5378)
                     /locus_tag="PLP95_03280"
     CDS             complement(4977..5378)
                     /locus_tag="PLP95_03280"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HPB44853.1"
                     /translation="MTDELLTAMGEFGFNEALGMEFLEASGDRVVATVEIGPRHHQPY
                     GITHGGVWCSAIETCASVGGAMWYGSQGQVVGVSNHTDFLKAVRDGVVTATATPIHRG
                     RLQQLWLVEIRTDDDRLVARGQVRLQNLPAS"
     gene            complement(5394..6206)
                     /locus_tag="PLP95_03285"
     CDS             complement(5394..6206)
                     /locus_tag="PLP95_03285"
                     /EC_number="4.2.1.17"
                     /inference="COORDINATES: protein motif:HMM:NF004840.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
                     /protein_id="HPB44854.1"
                     /translation="MPEGFETNQFETVRVEVADQVAVLTMNRPQARNALNSRLTRELW
                     LAVEEVAGSDEVDAIVLTGADPAFCAGLDLRELASGTAFRDEEPDPRPPGLPMRFFPV
                     VDVPIIAAVNGPAITGGFEVALQCTFIIASERARFADTHARVGILPGAGLTALLTEAV
                     GVRRATELSLTGNFLDAQTAYELGLVNRVVPHDELMATARATAADIVSNDQRAVRALV
                     AHYRGLQDQATLSDRFALEASLHHDFGGLANPADRVNGVIERGRTQQRIATP"
BASE COUNT         1046 a         2080 c         2045 g         1109 t
ORIGIN      
        1 cgcacccggt tctggcgcag cgtcgtggaa gggttgcgcg accgcgcccc cggccttgcg
       61 gttgcggttc ggctgtcggt gttcgacctg gtggcgtttc gagcgggggc ggacaacgtc
      121 ggcgaacccg tctgcgcggt cggcaccccc cggccattcg gcgcgaccga cgacggtctc
      181 gatgtcgacc tcgccgagcc cacgcgattt ctcgaactgg cgcgacaact gggggtcagg
      241 ctcgtgtgca cgaccgcagg cagcccgtac tacaacccac acatccaacg accggcgtac
      301 tttcccccat cggacggata ccgcccaccc gaggatcccc tggtcggagt ggccagacag
      361 atcgctgcga cggcagcact tcgacgtgcg tcgccggaca tgacgattgt gggttcaggc
      421 tattcctacc tacaggactg gttgcccgag gtcgccgagg ccgtggtcag ttccggtggg
      481 gcggacctga tcggcctggg tcggatgatg ctcagctacc cgcacctcgc cgccgacaca
      541 ctcgccggcg ccgaacgtaa ccggcgactg atctgccgca cgttttccga ctgcacgacc
      601 gccccgcgct ccgggctcgt gtcggggtgc tacccgctcg acgatttcta taacgagcgg
      661 cccgaacgca tcgaacttgc agcgatcaag cgccgcctgc gcgaaccctg agccaccgcc
      721 tgaaccaccg ccccgcctgt tctccgataa ccagtggtcg tgatcacgac cactggttat
      781 cggagaacag gcggtgagcc gatgctgtgt tgggtagttc tacctgggct gggtgatcca
      841 cttgcactgg tgtttagaat tgcccccatg cgtttgactc ccctcatgac cgcagccgct
      901 gttggtgctc tgctgaccgc tgctgcctgt accccttcaa ccggcggcgg ttcgtcgacc
      961 acggccccgc ccacgacccc aaccaccgag gcgccacagc cgctcgagcc cttcgaggtg
     1021 tccggcaccg ctgacaagac ggtgggatgc ttctcgggcc ttctgggcga cgacaaccag
     1081 gaggtgaacc tcggctccga cggttggtgg aacttcgggt tccagggatg cagcctgggt
     1141 gcgaccgaaa cggtcgccct gctcggcgtc gctccgcacg tcgccgacgg cttcaaggct
     1201 ccgacgggat atgagtgcaa gctggcgatc gccggcacct ccgatgaaac cccgtggtac
     1261 accaaccaga tcggctccac cgccgatacc ggtggcaagg ttttcctgag cagcagctcg
     1321 gcctggccga acacgaccac ttggacgatc acctgccggc cgctcacccc gatcccgatg
     1381 tgattccact gcgttgaggc ccgcggagtg acgatcgcta agcgggcacc gcagtcacct
     1441 caaccgtgag ctcacctcaa cggggtgccc gctagtgggc accccgtttg cgttggacga
     1501 gcccgccggc gaacagcaac gccaacccga ccatgagacc cacgatcgcc gaacacaggg
     1561 tgttcgcgac ccacccgagc acccctccca cgccggagac gtcgtggaaa ggttgctcga
     1621 tgcggtgcac gaggtcgtag ggcccgtgcc atccgagagc ggcagcgctc gacaacaaga
     1681 tgtgaccgcc gacccagagc atcgctgcgg ttcccacgac cgtcagcgtc gacagcacct
     1741 tgggcatggc ggcgacgagc gctcggccga ttcgttccga ccgcccgttg tctacggtgg
     1801 ccattcgcag cccgacatcg tccatcttca cgatgagggc gacagcgccg tacacgatcg
     1861 ccgtcatcag aaacgcgacg atgacaagga ccagcaggcg attgatgaac gggtcgtcga
     1921 tgacctcctt gagggccagc accatgatct cggtcgacag gatcaggtcg gttcggaccg
     1981 cgctcgagat cgtcttcttc tcggcctctt cgccggacat cgcggccggt aggtcgtcgt
     2041 cgtggtcgtg gccatggccg gtgattcggg cgtggatctt gtgtgcgccc tcataggcca
     2101 ggtagccgcc gccgaagatc aggatgacct cgatgagttt cggggccacc gcgctgagca
     2161 gcagcgccgc cggcaggatg aagaccagct tgtttcgaag cgaccccgcc gcgatcttct
     2221 tgatgatcgg gagctctcgc tcggcggcga gtccttgcac ataggtcggc gtgaccgctg
     2281 catcgtcgac gacgattccg acggtcttcg agctcgcctt cagcgcggcg gcgctggcat
     2341 cgtcgagggt tgccgcggcg agtttggcga gtgcggcgat gtcgtcgagt agggctacca
     2401 ggccaccggc cacgttcacg tcctcaggtt cgaaggttga cggtgagcga tacgataccc
     2461 acgcggtgcc cagcgcaggt gccacaaccg ggggcgtagg tatgggtgtt gcactggaga
     2521 agcttcgcga ccgggtggag cggcagggga tcgagttgcc ggggatgtac ggcgctttcg
     2581 acttcaacgc gattccggag cggctggcgc ttcgaccgga ggacgagacc gagctcgaca
     2641 ccgtgccgcg agtgaatcgg gccgacttct tggcggacca gggtctcatg gatctgatgg
     2701 ccgcagcgac gatgctcggc gatgcggtgt gcgacgccta tgtcgcgctg atcccgaccc
     2761 atgggatgca cggcctcatc acgatgttgc gcaccgcgtg ccagggccgc atcgacgacg
     2821 tcgaggaccc tccggacgaa ctgcgccgtt tcatcaccga actcgagacc gtgccggcgt
     2881 ggatcgacct cgaactcgtc gcagagggcg cccgtctgga acgaatctcc gccgcgctgc
     2941 tcgccccgtt cggggttcgc ggggcgttcc tcgccacctt cttgaactcc tatgcggcgc
     3001 ttccgatggc ggtgacgggt tcgttgagcg acaagcgttc cgccaagcgc gtcaacgaga
     3061 ccgcagcgtt cttcgcgtcg acggtgctgc cgggtggaat ggatcgcaat ggtccgggct
     3121 ttgaggccgc cgcgatggtg cgtctcatgc actcgatggt gcgatacaac gccttgcgcc
     3181 cggagcggaa ctgggatcag tcggtctatg gcgtgccgat tccccaggtc gaccagatgc
     3241 cggcggggct catcacttcc tttctcatgg cggtcgaggc ggttcatgcg aaacgcggct
     3301 acaccgacga cgaacgggcg atggtggaga tggcgcggta ccgctgttac ctgctcggcc
     3361 tgcccgaggt gttgttgccc gacgaccccg aagggctgat gcgagtgttt ctcgcccgtg
     3421 ccgcgaccct gcgccgcggc tacgacgact ccacttgtgg cgaattggtg cgtgggacgc
     3481 tcgccgccta tctgcgcccg gacacgacgc tcaagagccg cgtcgccgag tccttcgagc
     3541 ggagcttctc gacggccttc ttcattcgcg cctttctcgg caacgacgcc aaccgtgcgg
     3601 ccgaaatggg tgtgtcgttg acgggtgccg acaaagtgcg tgtcgcactc ggcgccccgt
     3661 tcatcttcgg tcggcaccgg atcgtgtcgc ggctcaacgg cgtggcacgt ctgcaaccgg
     3721 cgatcgatcg ctacatcgtt cgtcggctca accgtcgcct gcgcgactac ggacatgccg
     3781 agtatcgaag cgatgctgcg agctacaccg accggcgtgc gttggtgggc agataggcgc
     3841 cgtttgtatc ctcgtggccg acatcgccga tcggatcgag gattcatgac caagacgaac
     3901 ccgggcaact tcttcgagga cttcactctc gggcaggtca tcgaccacgc cgtgccccgc
     3961 accatcaccg agggtgaccg ggcgctgtac acgtcgctgt atccgacgcg attcgcgctg
     4021 ccgtcggcgg cgaccttcgc tgcgggggtc ggcctcgctg cgcctccggt cgaggaactc
     4081 gtcgggttcc acgtcgcctt tggcaagacc gtgccggacg tgtcgctgaa cgccgtcgcc
     4141 aacctcggct acgcggaatt gcggttccac cgcccggtgc tggtgggcga cacgttgcgt
     4201 tcgagttcgg aggtcatcgg cctcaaggag aactccaacg gcaagaccgg tgtggtctat
     4261 gtgcgttcga cggcgacgaa tcagcgcggc gaggtggcga tcgactgggc gcgttgggtg
     4321 atggtgaaca agcgcgacca ttccgccgcg gcccccgaga ccgtcgtgcc cgagttggcg
     4381 aaggcggtgt cgcccgagga cctgctgatc ccggacggcc tcgacttcac caggtacgac
     4441 ttctccgcgg ccggtgaacc tcaccgcttc gacgactacg aggtcggaga gaagatcgat
     4501 cacgtcgacg gcgtgaccct caccgacccc gaacacatgt tggcgactcg cctgtggcaa
     4561 aacaccgcca aggtgcattt caacaccgag gcccgccccg acggtcgccg cctcatctac
     4621 ggcggccaca tcatctcgat ggctcgggcg ctcagctaca acggcttggc gaacgcgcaa
     4681 ctcatcgcgg cgatcaacgc cggtgcccac accaatccgg ccttcgccga tgacaccgtg
     4741 tacgcgtggt ccgaggtgct cgacaaggcg gacacctcgg cgcctggcgt cggtgccatt
     4801 cgactgcgcc tggtggcgac aaagggtcgc gacccctcga tgacactcaa gggtgaggac
     4861 ggtaagtacg cctcgaacgt gctgctcgat ctggacctct gggcgttcat gccacgctga
     4921 cctgttctcc gataaccagt ggtcgtgatc acgaccactg gttatcggag aaccggtcag
     4981 gaggcgggga gattttgcag gcgtacctgc ccgcgggcga cgagccgatc gtcatcagtg
     5041 cggatctcga ccagccacaa ctgttgcaac cggcctcgat ggattggtgt cgccgtggcg
     5101 gtcaccacgc cgtcgcggac ggctttgagg aagtcggtgt ggttcgacac gccgacgacc
     5161 tgaccctgcg atccgtacca catggcgccg ccgacacttg cgcaggtttc gatggccgag
     5221 caccacacgc cgccgtgggt gatgccgtag ggctgatgat ggcgcgggcc gatctcgacg
     5281 gtggcgacga cccgatcgcc ggacgcctcg aggaactcca tcccgagggc ttcgttgaag
     5341 ccgaattcgc ccatcgcggt gagtagttcg tcggtcatgg tgtctccagt cggttaggga
     5401 gttgcgattc gttgttgggt tcgacctcgc tcgatgacgc cgttcacccg gtccgctgga
     5461 ttggcgagcc caccgaagtc gtggtggagc gaggcttcga gcgcgaaccg atccgacaag
     5521 gtcgcctggt cctgcagacc ccggtagtgc gcgacgagcg cccgaacggc tcgctggtcg
     5581 ttgctcacga tgtcggcggc cgtcgcacgc gccgtagcca tcagctcgtc gtgagggacg
     5641 acccggttga cgagccccaa ttcataggcg gtttgtgcgt cgaggaagtt gccggtgagg
     5701 gacagttcgg tggcccgtcg gacgccgacg gcctcggtca gcaacgcggt gagtcccgcc
     5761 ccgggcaaga tgccgacccg ggcatgggtg tcggcgaaac gggctcgttc ggaagcgatg
     5821 atgaacgtgc attgcagcgc cacctcgaac ccgccggtga tcgccggtcc gttgaccgca
     5881 gcgatgatcg gaacgtcgac gacgggaaag aagcgcattg gcagcccggg cggccgcggg
     5941 tcgggttcct cgtcgcgaaa cgccgtccca gaggcgagtt ctcgaaggtc gagcccggcg
     6001 cagaacgccg gatcggcacc ggtcaacacg atggcgtcca cctcatcgga accggcgacc
     6061 tcctcgacgg ccagccacag ttcgcgggtc agccgcgagt tcaacgcgtt gcgagcctgc
     6121 ggacgattca tcgtcagcac ggcgacctga tcggcgacct ccacccggac cgtctcgaac
     6181 tggttcgtct cgaaaccttc cggcacccga cctccctcac ggtgcgaccc atcgacatga
     6241 ccctgcgacg tggcccgttg cgaccctcag ccagcgagcc
//
LOCUS       DAOOMA010000073         6259 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_14863_length_6259_cov_3.575596, whole genome shotgun sequence.
ACCESSION   DAOOMA010000073 DAOOMA010000000
VERSION     DAOOMA010000073.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 6259)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6259)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6259
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14863_length_6259_cov_3.575596"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..441
                     /locus_tag="PLP95_03290"
     CDS             <1..441
                     /locus_tag="PLP95_03290"
                     /inference="COORDINATES: protein motif:HMM:NF015436.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter associated domain-containing
                     protein"
                     /protein_id="HPB44855.1"
                     /translation="ETKRVAELLREMQARKTHIAVVIDEYGGTAGLITLEDLLEELVG
                     EITDEFDTEVDWAEQLSSGAVVVHDASSNVDDINDRFDISLPEGDWDSVGGLVFSELG
                     RVPDVGDSIEVDGYRLTVEGMDGRRVERVMIEPYEAAEEVVEES"
     gene            438..1505
                     /gene="era"
                     /locus_tag="PLP95_03295"
     CDS             438..1505
                     /gene="era"
                     /locus_tag="PLP95_03295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HPB44856.1"
                     /translation="MSQPPMFDDPLDDEDDVDDSQFDDDYLIDPGEEGRTYSVEEFFA
                     SLGSSSSRSSSSSDASGSAGSDEGIDEVPEGFRSGFVSFVGRPNVGKSTLLNRILGRK
                     VSIVSDKPQTTRHQIRGVLNRPDAQIVFVDTPGIHKPRTLMGERLNDAAIDAAGAKAV
                     DVVCLLVDATARVGRGDAWVAERVPPDAIVVVNKCDLASKDQVVEQLLRASDFERSEY
                     FPVSAVTGEGVDALVDHLVSRLPEGPRWYPTDMVTDVEEAFWVAELVREKLLAVTRDE
                     LPHSIACRVTEWDWPRVRVEILVERESQKGIVIGKKGAVLKQVGIDARREMPEGTYLE
                     LHVKVDKDWQRRPKALDRLGY"
     gene            complement(1577..2146)
                     /locus_tag="PLP95_03300"
     CDS             complement(1577..2146)
                     /locus_tag="PLP95_03300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB44857.1"
                     /translation="MSSDLAESTVSIDPTIFQIDPARLTQAAKTIDADAQPDEAACPV
                     PRALAKAMGASACPFPVGGATTEPVRRSAADNAMRRLLRVHDRPAQVSERAAVAAFQK
                     SMLISAARCTLTYVVFPVLLPMVSFARGLGPVIGILIGTFALCCDVLTVRRFFAIDHK
                     WRWYFTAVATAVMTLLTVLLVEDLINLMS"
     gene            complement(2216..3757)
                     /locus_tag="PLP95_03305"
     CDS             complement(2216..3757)
                     /locus_tag="PLP95_03305"
                     /inference="COORDINATES: protein motif:HMM:NF013584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HPB44858.1"
                     /translation="MTDSPWKGDASSLVDAFRKGEHSPREEMEATLAAIERSELNAFS
                     HIDAEAALAAADSADVWKPFGGVPFAIKELDQVAGWPDTEASLVFKGRRATETSPYVE
                     RSINDGGFAPVGLTTASEFGGLNVSVTKINGITRNPWKPSQTVGGSSAGSAAAVSGGL
                     ITMASGGDGGGSIRIPAGYTGLLGMKGTFGRIPRGPAAPSRPNTVVHGAMVRSVRDIA
                     RFYDVTCGQHPWDPLSLPNPGDWEANLDAYDLRGRKVAVIPDLGSVTLDTGVDGRIRE
                     HADALIAAAGLVKVDLELDLHNITAEWAIGNLSTLLAELDDRWPACAPLLTDAIADGL
                     RIATSFYNLNTAAVAEQKRLQLQRQMADAFEQVDFIIAATNPGPAFPAEWETSNPLPE
                     ALTKIRSGAVGRGAQRALFGGIRAATGVAPRTPLRILAQAAERAPEMFEMGGLTIPSN
                     IYGNPAVSIPAGTVDGLPIGLQVLARHHQDALLLDLARCAERHLPWPLVAPTATSATE
                     VSAGA"
     gene            3834..4505
                     /gene="thpR"
                     /locus_tag="PLP95_03310"
     CDS             3834..4505
                     /gene="thpR"
                     /locus_tag="PLP95_03310"
                     /inference="COORDINATES: protein motif:HMM:TIGR02258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008664 - 2'-5'-RNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HPB44859.1"
                     /translation="MVAAMCGGMCGGTCGGAGSGVCHDEAMRGFFAVQLPDDVVEDLD
                     EFVDPRRDATDAAPLQRDRRPPWRWIRPDNWHLTLAFMESVPDARFGELVDLADAWAR
                     RHAPLRMRLEGCGAFANPAEASTLWMGVDAEAAVVLGEWSTALRGIANAAGAMPDGTR
                     FVPHVTVARSRRRRAAGRIVQSLDTYESRWFDVEWVTLIESHLGDGPARTPRYEDRAL
                     LHLGE"
     gene            complement(4554..>6259)
                     /locus_tag="PLP95_03315"
     CDS             complement(4554..>6259)
                     /locus_tag="PLP95_03315"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:NF024703.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HPB44860.1"
                     /translation="CRQRLDEALAPQAQIGLDGIVEAADRTPGGDVRPGSVPLRVLKE
                     LEAWADETATGDRADLAEEPKAWVWSAVSVGPRECPGAAKCPRGEDCFAELARRRAAS
                     ADVVVVNTHLYGLHLGSRGMILPDHDVVVFDEAHEVEDIMSATTGLEVGPGSVTSLAR
                     IVGNLIADDALLDHLDSDAARLSALLAPEVGSRLRRGLPDEVSDTLVALRERVTQVTN
                     TVRKIDDRAGTDTATRKERVTKAATGLLDDLATIVNPPGDSVLWVGGTPDHPKLELAP
                     LDIGATLDSLLWNATESGLDIDFGDAVDPDGHDGQVDDGTYGIPDTVILTSATIPDGL
                     AERLAIPPDTVEVIDVGTPFDFENHAVLYCAAHLPDPRSQNYGAAVAEELVRLINAAG
                     GRTLALFTSYRAMNEAADAVAEQVSLPILRQGDRPKPALVEAFSEDAESCLFATMGFW
                     AGIDVPGDSLRLVAIDKIPFPRPDEPLLQARRERAAAAGFSTIDLPRAATMLAQGAGR
                     LIRTATDRGVVAVLDPRLATKKSYRWQLISALPPMHRTKEFDEVRDFLASLAGSAPDA
                     S"
BASE COUNT         1005 a         1925 c         2278 g         1051 t
ORIGIN      
        1 gaaaccaagc gtgtggcgga gttgcttcgc gagatgcagg cgcgcaagac ccatatcgcg
       61 gtcgtgatcg acgagtacgg cgggaccgcc ggcctgatca ccctcgaaga cctgctcgag
      121 gaactcgtcg gcgagatcac cgacgaattc gacaccgagg tggactgggc cgaacaactg
      181 agttccggcg cggtggtcgt gcacgatgcc tcgagcaacg tcgacgacat caacgaccga
      241 ttcgatattt cgcttcccga gggggactgg gactcggtcg gtggattggt gttctcggaa
      301 ctggggcggg tgcccgatgt gggtgactcg atcgaggtcg acggataccg cctgaccgtc
      361 gaggggatgg acgggcgccg ggtcgagcgg gtcatgatcg aaccgtacga agccgccgaa
      421 gaagtcgtgg aggagtcgtg agtcagccac cgatgttcga cgaccccctc gacgacgagg
      481 acgacgtcga cgactcgcaa tttgacgatg attacctgat cgatcccggt gaggagggtc
      541 gcacctactc cgtcgaggag ttcttcgcct cgttggggtc gtcctcctcg cggtcgtcgt
      601 catcgtcgga cgcgtctggg tcggctggtt cggatgaggg gatcgacgaa gtgccggagg
      661 gattccggtc cgggttcgtc agtttcgtgg gacggccgaa cgtcggaaaa tccaccctgc
      721 tgaatcgcat tctcggtcgg aaggtgtcga tcgtgtcgga caagccccag acgacccgcc
      781 accagatccg aggggtgctc aaccggccag atgcccagat cgtcttcgtc gacacccccg
      841 gcattcacaa gccgcgcacg ttgatgggcg aacggctcaa cgacgcggcg atcgatgcgg
      901 ccggagccaa ggccgtcgat gtcgtgtgcc tgctcgtcga tgcgacggcc cgggtcggtc
      961 gtggcgacgc ctgggtggcc gaacgcgttc cgccggacgc gatcgtcgtg gtgaacaagt
     1021 gcgacctggc ctcaaaggat caggtggtcg agcaactgct tcgggcgagc gacttcgaac
     1081 gctcggagta ctttcctgta tcagccgtca ccggcgaggg ggtggacgcc ctggtcgatc
     1141 acctcgtgag tcgtctaccc gagggcccgc gctggtatcc gaccgacatg gtcaccgacg
     1201 tcgaagaggc gttctgggtt gcggagttgg tacgcgagaa gttgctggcg gtcacccgag
     1261 atgagttgcc gcactcgata gcgtgtcggg tgaccgaatg ggattggccg cgggtccggg
     1321 tggagatcct cgtcgaacgg gagtctcaga aggggatcgt catcggcaag aagggcgcgg
     1381 tgctcaaaca ggttgggatc gacgcccgcc gtgagatgcc cgagggcacc tatctggaac
     1441 tgcacgtcaa ggtcgacaag gactggcaac gtcggcccaa ggcgctcgac cgactcggct
     1501 actgagaccg ggtcgcctac tgagaccggg tcggctaccg agaccgggtc ggctaccgag
     1561 accggacccg aggccttcaa ctcatgaggt tgatcagatc ctcgacgagc aacacggtca
     1621 acagggtcat cacggcggtg gccacggcgg tgaaatacca gcgccacttg tggtcgatgg
     1681 cgaagaaccg gcgaactgtc aacacgtcac agcacagcgc gaacgttccg atcagaattc
     1741 cgatgacggg gcctaggcca cgagcgaagc tgaccatggg caacagcacc gggaacacca
     1801 cgtaggtgag ggtgcagcga gccgccgaga tcagcatcga cttctggaag gcggcgaccg
     1861 cggcccgctc gctgacctgg gccggccggt cgtggactcg caacagcctg cgcatcgcgt
     1921 tgtcggcagc cgaccgccgc acgggttctg tggtagcgcc cccgaccggg aacgggcacg
     1981 cgcttgctcc cattgctttg gccagtgcgc gcgggacggg gcaagcagcc tcgtcgggct
     2041 gggcgtcagc gtcgatcgtc ttggccgcct gggtcaagcg ggcggggtcg atctggaaga
     2101 tggtggggtc gatcgaaacg gtggattcgg ccaaatcgga tgacatggaa agagcctcca
     2161 gggaccccag cagtctacgg cggtgaaccg gggggcgacg aaccgggccg tgggtttagg
     2221 cgccggcgga cacctcggtg gccgatgtcg ccgtgggagc gacgaggggc cacggcaggt
     2281 gacgctcggc gcaccgcgcg aggtccaaca acagggcgtc ttgatggtgt cgggcgagca
     2341 cctgcaaccc gatgggaagc ccatcgacgg tgccggcggg aatcgacacc gcagggttgc
     2401 cgtagatgtt cgacggaatg gtcagcccgc ccatctcgaa catctccggg gcgcgctcgg
     2461 cggcctgggc gaggatgcgg aggggggttc tgggtgccac gccggtggcc gcgcggatgc
     2521 cgccgaacaa tgctcgttga gcgccccgtc cgacggctcc ggagcggatc ttcgtgagcg
     2581 cctccggcag ggggtttgac gtctcccact cggcggggaa ggcgggccct gggttggtcg
     2641 cggcgatgat gaagtcgacc tgttcgaacg cgtcggccat ctgacgttgc agttgcaggc
     2701 gcttttgttc ggccaccgcg gcggtgttga ggttgtagaa cgacgtcgcg atccggaggc
     2761 cgtcggcgat ggcgtcggtc aagagtgggg cgcacgccgg ccaacgatcg tcgagttcgg
     2821 ccagcagcgt tgacaggttg ccgatcgccc actcggcggt gatgttgtgc aggtcgagtt
     2881 cgaggtcgac cttcaccagg cccgcggcgg cgatgagcgc gtcggcgtgt tcgcgaatcc
     2941 ggccatccac gccggtgtcg agggtgaccg aaccgagatc ggggatcacc gccaccttgc
     3001 ggccgcgcag gtcgtaggcg tcgaggttcg cctcccagtc gccgggattg ggcagcgaca
     3061 gcgggtccca cgggtgttga ccgcacgtga cgtcgtagaa acgggcgatg tcacggaccg
     3121 agcgaaccat cgcgccgtga acgacggtgt tgggccgtga cggtgccgcg gggccgcgcg
     3181 gaatgcgtcc gaaggttccc ttcattccca gcagaccggt gtatccggcg ggaatgcgga
     3241 ttgacccgcc gccgtcgccg cccgaggcca tggtgatgag cccaccggaa accgccgccg
     3301 cgctacctgc cgatgagcca ccgacggtct gagacggctt ccacgggttg cgggtgatgc
     3361 cgttgatctt ggtgacgctg acgttcaacc cgccaaattc gctcgccgtg gtcaacccga
     3421 ccggagcgaa cccgccgtcg ttgatcgatc gctcgacgta gggggaggtc tcggtcgctc
     3481 ggcgcccttt gaacaccaac gaggcttcgg tgtcgggcca cccggcgacc tgatcgagct
     3541 ccttgatggc gaacggcacg ccaccgaagg gcttccacac gtcggcggaa tcggcggccg
     3601 cgagcgccgc ttcggcatcg atgtggctga atgcgttgag ttcgctgcgt tcgatggcgg
     3661 cgagggtggc ctccatttct tctcgcggcg aatgttcccc tttgcggaac gcgtcgacga
     3721 ggctcgacgc atcccctttc cacggcgaat cggtcatggc gcaaagtgtt gcaccgcgac
     3781 ggttcccgga tcgaactggc ccgcccgcga tgccgatccc gagggtggca gcgatggtgg
     3841 cagcgatgtg cggcgggatg tgcggcggga cgtgcggcgg cgctggcagc ggggtgtgtc
     3901 acgatgaggc gatgcgtggg ttcttcgccg tgcagctgcc cgatgacgtc gtcgaggacc
     3961 tcgacgagtt cgtcgacccc cgtcgcgatg ccaccgacgc ggcaccgctg cagcgggatc
     4021 ggcgcccacc ctggcgttgg attcgacccg acaactggca cctcacgttg gcgttcatgg
     4081 aatcggtgcc ggatgctcgt ttcggggaac tggtcgacct ggccgatgcc tgggctaggc
     4141 gtcacgcgcc gttgcgcatg cgcctcgagg ggtgcggcgc gttcgcgaat ccggcggagg
     4201 catccacctt atggatgggg gtcgacgcgg aggccgcggt cgtgctcggc gagtggtcga
     4261 cggcgcttcg cggcatcgcc aatgctgcgg gtgccatgcc cgacgggacg cgctttgtcc
     4321 cgcacgtcac ggtggcgagg tctcggcgcc ggcgtgcggc gggacgtatc gtgcagtcgc
     4381 tcgacaccta tgagtcgagg tggttcgacg tcgagtgggt gaccctgatc gagtctcatc
     4441 tcggtgacgg tccggcgcgt acccctcgat acgaggaccg ggcgctgtta cacctcggcg
     4501 agtgaggcag cggggcgctg gggtcagccc gcgctggatc agctcgcgtc ggatcagctc
     4561 gcgtcgggcg ccgatccggc caacgatgcg agaaagtcgc gcacctcatc gaattccttg
     4621 gtgcgatgca tcggaggtag ggcggagatg agttgccagc gatacgactt cttggtggcg
     4681 aggcgcgggt cgagcaccgc gacgacgccg cggtcggtgg cggttcggat gagtcggccg
     4741 gcgccctgag cgagcatcgt cgcggcgcgg gggagatcga tcgtggagaa tcccgcggct
     4801 gcggcgcgtt cgcggcgtgc ctgcaacaac ggctcgtcgg ggcgagggaa cgggatcttg
     4861 tcgatcgcga cgaggcgcag cgagtcgccg ggaacgtcga tgccagccca gaagcccatc
     4921 gtggcgaaca ggcacgactc ggcatcctcg ctgaaggcct ccacgagcgc cggcttcggt
     4981 cggtcgccct ggcgcagaat cggcaaggaa acctgttcgg cgaccgcgtc ggcggcttcg
     5041 ttcatcgcgc gatagctggt gaacagcgcc agggttcggc caccggccgc gttgatgagt
     5101 cggacgagtt cctcggcgac ggcggcgccg tagttttgcg accgggggtc ggggaggtgt
     5161 gcggcgcagt acagcaccgc gtggttctcg aagtcgaacg gggtcccgac gtcgatcacc
     5221 tcgacggtgt ccggggggat ggcgaggcgt tcggcaagtc cgtcggggat ggtggcggag
     5281 gtcaaaatga cggtatccgg gatgccgtag gtgccgtcgt cgacctggcc gtcgtggccg
     5341 tcggggtcga cagcgtcgcc gaagtcgatg tcgaggccgg attcggtcgc gttccacagc
     5401 agcgagtcga gggtcgcccc gatgtcgagg ggtgccagtt cgagcttcgg atggtccggg
     5461 gtgccaccga cccacaacac cgagtctccg ggtgggttga cgatggtggc gaggtcgtcg
     5521 agcagcccgg tcgcggcttt cgtcacgcgt tcctttcggg tcgcggtgtc ggttccggcg
     5581 cgatcgtcga tcttgcgcac cgtgttggtc acctgggtca cccgttcgcg cagcgccacc
     5641 agcgtgtcgg acacctcgtc gggcaggccg cgccgcagcc gtgagccgac ctcgggggcc
     5701 agtaacgcgc tgagccgcgc ggcgtcggag tcgagatggt cgagcagtgc gtcgtcagcg
     5761 atcaggttgc ccacgatgcg ggccaacgag gtgaccgatc ccggtccgac ctcgagcccg
     5821 gtcgtcgcgc tcatgatgtc ttcgacctcg tgggcctcgt cgaagaccac gacgtcgtgg
     5881 tccggcagga tcatgccccg cgagccgagg tggagcccat agagatgggt gttcaccacg
     5941 acgacgtcag cgctcgccgc ccgccggcgt gccaactcgg cgaaacagtc ctcgccgcgc
     6001 gggcacttgg ccgcgcccgg acattccctc ggtcccaccg ataccgccga ccagacccac
     6061 gccttgggct cctcggcgag gtcggctcga tccccggtcg cggtctcgtc cgcccacgcc
     6121 tcgagttctt tgaggacccg caggggaacc gaccccggac gaacgtcccc gccaggggtg
     6181 cggtcggcgg cctcgacgat tccgtcgagg ccgatctgtg cctggggtgc gagcgcctcg
     6241 tcgaggcgtt ggcgacaca
//
LOCUS       DAOOMA010000074         6221 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_14995_length_6221_cov_3.243756, whole genome shotgun sequence.
ACCESSION   DAOOMA010000074 DAOOMA010000000
VERSION     DAOOMA010000074.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 6221)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6221)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6221
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14995_length_6221_cov_3.243756"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            126..734
                     /locus_tag="PLP95_03320"
     CDS             126..734
                     /locus_tag="PLP95_03320"
                     /inference="COORDINATES: protein motif:HMM:NF036410.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5317 family protein"
                     /protein_id="HPB44861.1"
                     /translation="MAILPTIVALALGVGLGLWLGGSVERARSIRPDLARTAAVALAV
                     QILAQVIGLSGGWAVFVDLVSKGALIAFAVFNLRIGGMVVLAAGLSLNWLVTILNWGM
                     PTSRAALERAGLIDPGTTGTVHLTGARHVADGDRLSFLGEVIALPTHQVISIGDVLTL
                     VGVVLVIMAVLRARVLPAESSPLRLDQITLPRGPRGLSRRHA"
     gene            809..1108
                     /locus_tag="PLP95_03325"
     CDS             809..1108
                     /locus_tag="PLP95_03325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB44862.1"
                     /translation="MSEPPTNPADAEVSADDGIEAKRARIKALSAKANRYGYLAILLA
                     MVVFVIGFVTSFSLWVTTTILALLVLSGLLLVPAMIFGYGVKYAEQDERGEKFHY"
     gene            1203..1808
                     /locus_tag="PLP95_03330"
     CDS             1203..1808
                     /locus_tag="PLP95_03330"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HPB44863.1"
                     /translation="MRLPAAERRAQLLKTAVDVFAANGFHDTSMNVIAEAAGVTKPVL
                     YQHFASKRALYLALLAELGDELRQTIAKATADAPGPRQQIEYGFVAYFTYVEGNANAF
                     RVLFGAGTRRDEEFAEAVQQVEDSIAELAAELIDVDGLSLERRRLLGNGIVGLAEGAC
                     RYWLANAVEVDATTLATEVSWLAWAGLRGIRSEVGTPSPTR"
     gene            complement(1812..2006)
                     /locus_tag="PLP95_03335"
     CDS             complement(1812..2006)
                     /locus_tag="PLP95_03335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB44864.1"
                     /translation="MSESADISYTCPRCSAEVAEAYYGPCRSCRQLLRDQYAGLEQDI
                     EAEEYAPKMNVTPNAVALKE"
     gene            2053..3396
                     /locus_tag="PLP95_03340"
     CDS             2053..3396
                     /locus_tag="PLP95_03340"
                     /inference="COORDINATES: protein motif:HMM:NF013714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HPB44865.1"
                     /translation="MTHDERVRSIVAQVRAANATPGPTHIDKGGVHHVVPLPGDRRFA
                     SRSIDTSALTNIIDIDTDALTCTAEPGVTFEDLVRATLPLGLAPTVVPELRGITLGGA
                     VAGCSVESMSFRYGGFHDSCLSYEVIGGDGEVHELSTTHRPELFHHLHGSYGTLAILT
                     RLTFRLVPAKPFVAVEYRHHTDLAKFRADMSAHCDLDGNPDNDFVDGIIHAPDQLTLV
                     VGRFTEAPGEGDLATYSGEQMFFREAARRSRDVMTAEEYFFRYDRDCHWMTATVPPLQ
                     FKPLRRAVGRWFLGSTNLIAWSNRVAPVSRRLLRRPEVVCDIFLPKSNFERFWEWYCR
                     VFDFWPLWVVPYRPATLYPWIGPHVREGFAEGDLFIDFAVYGKRNTERHRDYSELLEA
                     ATFDLGGIKTLIGRNHYSRERFWQIYDRPAYEKAKVELDPTGLFPDLYDKLGNVS"
     gene            3570..4151
                     /locus_tag="PLP95_03345"
     CDS             3570..4151
                     /locus_tag="PLP95_03345"
                     /inference="COORDINATES: protein motif:HMM:NF022589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAP family protein"
                     /protein_id="HPB44866.1"
                     /translation="MTGPAFGVAWVVGLSTITVAFVATMSAAEGEGDLTDPANAATTG
                     IGWAKLLVGGAFLALAVHKWRTSPRDGEEPILPAWMDNTSDISGSKAAGFGLLLSAAN
                     PKNLALVGAAAAAIVEQTGGATPAVEAIVVFVALGSLSVLIPIAASIVAPQRATVALE
                     DLRHFMTRNNTAIMMTVLVILGLKMVGDGLKIF"
     gene            complement(4227..5063)
                     /locus_tag="PLP95_03350"
     CDS             complement(4227..5063)
                     /locus_tag="PLP95_03350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPB44867.1"
                     /translation="MNPRDKVASDERSATAPSAGVDAPNSVMWAIRDSWNEATRHLRS
                     IPRSPELLMFTVLQPVMFVLLFYYVFGGAIQVPGSYAQFLVPGILAQTVVFGSSFTGV
                     GLAEDMSKGFVDRLRSLPINQSAVLVGRTLSDLARNSLGALIIIVIGFALGFRFNGGV
                     VDGVVATLLFLAFAYAFSWIQALVGLSVSSVEAANSAGFIWMFPLTFVSSAFVPTESM
                     PGWLRQIADANPFTQVTNAGRALYAGTDPGNSVWIAALWALGITVVFAYLATSKFKRT
                     SH"
     gene            complement(5060..6016)
                     /locus_tag="PLP95_03355"
     CDS             complement(5060..6016)
                     /locus_tag="PLP95_03355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HPB44868.1"
                     /translation="MTAMPLIEASGLVKNYGETLALAGLDLEVEAGMVLGMLGPNGAG
                     KTTTVRILTTLLKPDAGTAFIDGIDVVAEPRRIRSRIGLTGQYAAVDERLTARENLDL
                     IGRFFRLSSSEAAGRANELLDRFRLDEAGDRLVKGFSGGMRRRLDIAMSLVARPKVLF
                     LDEPTTGLDPRSRLDMWDLIDELQREGMTTLLTTQYLEEADRLADEIVVIDHGLVIAR
                     GTAAELKALIGGSKAVIEPSGATTLTEVADALASVRGGAEPTSEGGAIMVPVRLGTTT
                     PDVVRLLDAAALAVVDVAITSPTLDDVFLALTGHASDAEVSA"
BASE COUNT         1076 a         2087 c         1972 g         1086 t
ORIGIN      
        1 ccggacccgc tgagcgaggt gagaagttga tcatcggaca tgggtgcaca ggctagttcc
       61 gtggctggaa acgttggcga tgaggcgtcc accgcgaacc cgcaaaccgc cgatagattc
      121 aacccatggc gatcctcccc accatcgtcg ctctcgcgtt gggagtcggg ctcggcctct
      181 ggctgggcgg ttcggtggaa cgagcgcgaa gcattcgccc cgacctcgcc cgaaccgcag
      241 cggtcgcgtt ggcagtgcag atcctcgctc aagtcatcgg actctccggt ggatgggcgg
      301 tgttcgtcga cctggtgtcg aagggcgcgc tgatcgcatt cgccgtgttc aacctgcgaa
      361 tcggcggcat ggtggtcctc gccgcagggc tctcgctgaa ctggttggtg accatcctca
      421 actgggggat gccgacgagt cgcgccgcgc tcgaacgcgc cggcctgatc gacccgggaa
      481 cgaccggcac ggtgcacctc accggcgccc ggcacgttgc cgacggcgac cgcctcagct
      541 ttctcggcga agtcatcgcc ctgccgaccc accaggtcat ctcgatcggc gatgtgttga
      601 ccctcgtcgg agtcgtgctc gtcatcatgg cggtgctccg agcccgggtt ctgccggcgg
      661 agtcatcacc gttgcgcctc gatcagatca cgctgcctcg aggccctcga ggcctgtcgc
      721 gtcgccacgc ctgagcgccg gtcggccgtt cgtgttcgat tgatcgactg tttctcgtcg
      781 tcatcgccca cgtgccagag tggagcccgt gagcgaaccg ccgacgaatc cagccgatgc
      841 cgaagtttcc gcggacgacg ggatcgaggc caaacgagcc cgcatcaaag cgttgtcagc
      901 gaaggcgaac cgatacggct atctggcgat cctgttggcg atggtcgtgt tcgtcatcgg
      961 gttcgtcacg tcgttctcgc tctgggtcac cacgacgatc ctggcgttgt tggtgctgtc
     1021 cgggctgctg ttggtcccgg cgatgatctt cgggtacggc gtcaaatacg ccgaacagga
     1081 cgaacgaggc gagaagttcc actactgaac cgccacggct gacgaacgcc ccatcagccc
     1141 ggcgggaaac gtgacgtacg gctcagcggg atccgtagtt accgcatggt aacttacggg
     1201 ccatgcgcct acccgccgcc gaacgccgtg cgcaactgtt gaagaccgcc gtcgacgtct
     1261 tcgcggccaa cgggttccac gacacttcga tgaatgtcat cgccgaggcg gccggggtca
     1321 ccaagccggt gctctaccag catttcgcct cgaaacgggc gctctacctc gcgttgttgg
     1381 ccgaactcgg cgacgaactg cgtcagacga tcgccaaggc aaccgccgac gctccggggc
     1441 cacgccaaca gatcgaatac gggttcgtcg cctacttcac ctatgtcgag ggcaatgcca
     1501 atgcctttcg ggtgctcttc ggcgcgggca cccgccgcga cgaggagttc gccgaggcag
     1561 tgcagcaggt cgaggattcg atcgcggaac tcgcagccga actcatcgac gtcgacggcc
     1621 tcagcctgga acggcgtcgg ctgctcggca acgggatcgt cgggctcgcc gagggggcct
     1681 gtcgttactg gctcgccaac gcggtcgagg tcgacgccac gacgctcgcc accgaggtca
     1741 gttggttggc ctgggccggc cttcgcggca ttcgcagcga agtcgggacc ccgtcgccaa
     1801 cccgctgagc gctactcctt gagcgcgacc gcgttgggcg tcacgttcat cttcggtgcg
     1861 tactcctcgg cctcaatgtc ctgttcgagc cccgcgtact gatcgcgcag cagttggcga
     1921 cacgaacggc atggtccgta atacgcctca gccacctcgg cgctgcatcg ggggcaggtg
     1981 taggaaatat ctgcggactc gctcacggct ccaccttcgc gcatccgggc gtcgacgcgc
     2041 gaccatggtg tggtgaccca cgacgaacga gtacgaagca tcgtggcgca ggtgcgcgct
     2101 gccaatgcca ccccggggcc aacccacatc gacaaaggcg gggtgcacca cgtcgttccg
     2161 ctgcccggtg accgccggtt cgcctcccgg tcgatcgaca cctcagcact caccaacatc
     2221 atcgacatcg acaccgatgc cctgacctgt accgccgagc ccggagtcac cttcgaggac
     2281 ttggtccgcg ccacccttcc cctcgggctc gcaccgaccg tggtgcccga actgcgcggc
     2341 atcaccctcg gcggtgcggt cgccggatgt tcggtcgagt cgatgtcgtt tcgctacgga
     2401 ggattccacg actcgtgcct gtcctacgag gtgatcggcg gcgacgggga ggtgcacgaa
     2461 ctgtccacca cccatcgccc ggagttgttc catcaccttc acggttccta cggcaccctc
     2521 gccatcctca cccggctgac ctttcggctt gtgccggcga aacccttcgt cgccgtcgag
     2581 taccgtcacc acaccgacct cgcgaagttc cgcgccgaca tgtcggcgca ctgtgacctc
     2641 gacggcaacc ccgacaacga tttcgtcgac gggatcatcc acgcccccga ccagttgacg
     2701 ctggtggtcg gcaggttcac cgaggcgccc ggcgagggcg acctggcgac ctattcgggc
     2761 gagcagatgt tctttcgcga ggccgctcgc cgctcacgcg atgtgatgac cgccgaggag
     2821 tacttcttcc gctacgaccg cgactgccac tggatgaccg caaccgttcc gccgctgcag
     2881 ttcaaaccac tgcgccgtgc tgtcggccgc tggttcctcg gctcgacgaa cctgatcgcc
     2941 tggtcgaacc gagtggcacc ggtgtcgcgg cgcttgctgc gacgccccga ggtggtgtgc
     3001 gacatcttcc tgcccaaatc gaacttcgaa cgcttctggg agtggtactg ccgagtgttc
     3061 gacttctggc cactgtgggt cgtcccctac cgtccggcga cgctgtatcc ctggatcggc
     3121 ccccatgtcc gcgagggctt cgccgaaggg gatctgttca tcgacttcgc cgtctatggg
     3181 aaacgcaata ccgaacgcca ccgggactac tccgaactcc tggaggccgc caccttcgac
     3241 ctcggcggga tcaagaccct gatcggccgg aaccactaca gccgagagcg gttctggcag
     3301 atctacgacc ggccggcgta tgagaaggcc aaggtcgaac tcgacccgac cggattgttc
     3361 cccgacctct acgacaagct cggcaacgtc tcctgagcga caatactgag gtggctccgc
     3421 atcggagcca cctcggcatg aaagccgctc gacaggagca tcgccatgat cgactcgctc
     3481 ggagatgtcc tcccgttcgc cgccggggtt gcgctcagtc cgatcccgat catcggcgtg
     3541 atctggtgct cagtgccgac cgttcacggg tgaccggccc ggcgttcggg gtggcatggg
     3601 tggtggggct gtcgacgatc accgttgcgt tcgttgctac catgtccgcc gccgaaggcg
     3661 agggtgatct caccgacccg gcgaatgcag ctacgaccgg gatcgggtgg gccaagctgc
     3721 tggtcggcgg ggcgttcttg gccctcgcgg tccacaaatg gcgcactagc cctcgcgacg
     3781 gggaggaacc gatacttccg gcgtggatgg acaacacctc cgacatctct ggttcgaagg
     3841 ccgccgggtt cgggctgctg ttgtccgctg cgaatccgaa gaacctcgca ctcgtcggcg
     3901 cagcggcggc cgcgatcgtc gaacaaaccg gcggcgcaac cccagcggtg gaggccatcg
     3961 tcgtgttcgt cgcgctggga tcgctgagcg tgctcattcc gattgccgca agcatcgtcg
     4021 ctccgcagcg cgccaccgtc gcactcgagg accttcgcca tttcatgacc cgcaacaaca
     4081 cggcgatcat gatgacggtg ctggtgatcc tcgggctgaa aatggtgggt gacggactca
     4141 agatcttctg agcccatccg tccgaccacc gcagagcggt agcaccgaga cggtgatgcc
     4201 gagcggtgcc cgagtgcgtt gcgacctcag tggctcgtgc gtttgaactt cgacgtggcc
     4261 agataggcga agaccaccgt gattccgagc gcccacaacg ccgcgatcca cacgctgttg
     4321 ccggggtcgg tgcccgcgta cagtgcgcgc ccggcgttgg tgacctgggt gaacgggttg
     4381 gcgtcggcga tctgacgtaa ccagcccggc atcgactcgg tcggaacgaa cgccgacgac
     4441 acgaaggtca gggggaacat ccagatgaac cccgccgagt tcgccgcttc caccgatgac
     4501 accgacaacc cgacgagcgc ttgaatccaa ctgaacgcgt aggcgaacgc gaggaacaac
     4561 aacgtggcca cgacgccatc gacgacgccg ccgttgaaac ggaacccgag ggcgaatccg
     4621 atgacgatga tgatcaacgc tccgaggctg ttgcgggcca ggtccgacaa ggtacggccg
     4681 accagcaccg ccgattggtt gatcggcaat gaccgaaggc gatcgacgaa gcccttcgac
     4741 atgtcctcgg ccaacccgac tccggtgaag gacgaaccga atacgaccgt ctgcgccagg
     4801 atgccgggaa cgaggaactg cgcgtaggag ccgggaacct gaatcgcccc gccgaacacg
     4861 taatagaaca acagcacgaa catcaccggc tgcaacacgg tgaacatcaa cagctccggg
     4921 ctgcggggaa tggatcgcag gtgtcgcgtc gcctcgttcc agctgtctcg aatggcccac
     4981 atcacgctgt tgggcgcatc gactccggcg gagggcgccg tcgccgacct ttcgtcggat
     5041 gcaaccttgt ctcgcgggtt catgccgaca cctccgcgtc gctcgcgtgt ccagtcagtg
     5101 ccaggaatac gtcgtcgagg gtgggcgacg tgatggccac gtccacgacg gcgagcgcgg
     5161 cggcgtcgag gagccgcacg acgtcggggg tcgtcgtgcc cagccgtacc ggcaccatga
     5221 tcgctccccc ctcgctggtc ggctcggccc cgccgcgcac cgacgccaac gcgtccgcca
     5281 cctcggtcaa cgtggtcgcc cccgacggct cgatcaccgc cttcgatccg ccgatgagtg
     5341 ccttcagttc cgcagcggta ccgcgtgcga tgacgagccc atgatcgatg acgacgatct
     5401 catcggcgag acggtcggcc tcttcgaggt actgggtcgt gagcagcgtc gtcatgcctt
     5461 cgcgctgtaa ctcgtcgatc agatcccaca tgtcgagtcg gctgcggggg tcgaggccgg
     5521 tggtcggctc gtcgagaaac agcacctttg ggcgggcgac cagactcatc gcgatgtcga
     5581 gtcgccgacg cattccaccg gagaagccct tgaccaaccg atctccggcc tcgtcgaggc
     5641 ggaaccgatc gagcagttca ttggcccggc cggcggcctc ggaggacgag aggcggaaaa
     5701 agcggccgat gaggtcgagg ttctcgcggg cggtgaggcg ttcatcgacc gccgcgtact
     5761 ggccggtgag accgatccgt gaacggatcc gtcgcggttc ggcgaccacg tcgatcccgt
     5821 cgatgaacgc cgtgccggcg tcgggcttga gcagggtggt gaggatccgc acggtcgtgg
     5881 tcttgcccgc cccgttcgga ccgagcatcc ccagcaccat tccggcctcg acctcaaggt
     5941 cgaggccggc aagggccagg gtttccccgt agtttttgac gagcccactg gcctcgatga
     6001 gtggcatggc ggtcatgcct accgaccatt acccggcaga tccgcggaat ttccagacaa
     6061 cgttttcgtc tggtttcgac ggcccgcagg cgcgcccgcc gagttccggt gccgcggcac
     6121 gaaaccgccg cgatcgcgcg ttacggaacg cgaactcccg acgccgaacg ggcgatgacg
     6181 aggcgctgga tctcggaggt gccctcaaag atcgtcatga t
//
LOCUS       DAOOMA010000075         6216 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_15012_length_6216_cov_3.749878, whole genome shotgun sequence.
ACCESSION   DAOOMA010000075 DAOOMA010000000
VERSION     DAOOMA010000075.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 6216)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6216)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6216
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15012_length_6216_cov_3.749878"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(145..489)
                     /locus_tag="PLP95_03360"
     CDS             complement(145..489)
                     /locus_tag="PLP95_03360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB44869.1"
                     /translation="MSAEGFLQQLPDVDPQETAEWIDSLESVAATRGKPRASFLIGRM
                     EMRAEEMGIDNSGLVTTPYINTIPPNEQAWFPDDTDLERRIRAFIRWNALAMVSRSTS
                     CRASFSVPLPFS"
     gene            559..1119
                     /locus_tag="PLP95_03365"
     CDS             559..1119
                     /locus_tag="PLP95_03365"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: protein motif:HMM:NF012304.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease H"
                     /protein_id="HPB44870.1"
                     /translation="MVQVVYTDGACRGNPGPGGWAWALVEEGRGAGLSDRWASGAEAH
                     TTNQRMEVMAAHQAVLAVDGPIEVRSDSTYVVKCFNDRWYRGWLARGWLNSQKQAVAN
                     RDLWEPFIDVVLARGDVTFTWVKGHGTDRMNQFVDRLAVEASQRQRGAVGGEAGRALA
                     GESAALLLGGRATGEPGAIVAQESLF"
     gene            complement(1202..1480)
                     /locus_tag="PLP95_03370"
     CDS             complement(1202..1480)
                     /locus_tag="PLP95_03370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB44871.1"
                     /translation="MATTGHSEIIMSPECRACYDLGIEPLTDFRSDAGWRTVVSAHRA
                     IVKRYHPDRFVERSAEEMAMAVDRTTEANCAYTLLQTARRTEAPQSWY"
     gene            1595..2371
                     /locus_tag="PLP95_03375"
     CDS             1595..2371
                     /locus_tag="PLP95_03375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HPB44872.1"
                     /translation="MDITGSSAIVTGGASGLGEATSRLLAERGVKVVVADLDRQQDRG
                     DALANEIGGLFVACDVTDAEQVQAAVDAAVEMAPLKSLVNCAGIGWASRTIGKNGEPH
                     DLEIFRKVIDINLVGSFNCIRLAASAMNRNEGDADGAKGAIVNTASVAAYDGQIGQAA
                     YSASKGGVVGMTLTIARDLAAAGIRVNTIAPGLIDTPIYGEGEAAEAFKEALGKSVLF
                     PHRLGVSAEFASLALELLTNSYMNAQDIRLDGGIRMPPKA"
     gene            complement(2470..3099)
                     /locus_tag="PLP95_03380"
     CDS             complement(2470..3099)
                     /locus_tag="PLP95_03380"
                     /inference="COORDINATES: protein motif:HMM:TIGR01490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IB family hydrolase"
                     /protein_id="HPB44873.1"
                     /translation="MSIQHFPRSGLAAWDFDETITTRDTLVPFLTFIAGRSAVAKSLA
                     SHAPVLARGLRSSTQRDRAKELVLSDVLRGRHLDEVEAAGLQYARTLPRRFRRESLER
                     IAWHRAQGHRQVIVSASLVYYLRPIAEDLGFDDVIGVDMAVGDDGRLTGDLVGNNVRK
                     AEKAARLSAWIDAAGLIDPEVWAYGDSSGDVELLAMATHPTWVGRRATK"
     gene            complement(3121..3588)
                     /locus_tag="PLP95_03385"
     CDS             complement(3121..3588)
                     /locus_tag="PLP95_03385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012225929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HPB44874.1"
                     /translation="MTDDAKQVSASREIAAAPAELFKIVADASFHPVIDGSGTVRSAT
                     GKSEPLKLGSKFGMNMKMFLPYRLTSTVVEFEQDRLIAWQHPGKHIWRYTFEPLDDVD
                     GTPRTLVTETFDWRPSPIKFYIEGLGWPKRHKVNMAKTLENLDTYVTNRSSAL"
     gene            complement(3616..4500)
                     /locus_tag="PLP95_03390"
     CDS             complement(3616..4500)
                     /locus_tag="PLP95_03390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01777 family oxidoreductase"
                     /protein_id="HPB44875.1"
                     /translation="MRVAVSGATGLIGSRLVAELRSVGHEAVTISRRPGVDTIVWDPD
                     RGVLADGAIDGFDAVVNLAGEPIGAKRWTTEQKRRIVDSRVRSTELLVDAMARSSRPP
                     TRFLSGSAIGYYGDCGAQVLTENSPAGSGFLADVVRRWEAAAAPTVELGISTALLRTG
                     IVLSAEGGALKQQMLPFKLGLGGRSGNGRQFQSWIHLDDHVRAIVWLLERPVAGPVNL
                     TAPNPVTNAEFASTLASVLRRPCGVIPQFGPRLLFGPELADTLLLESQRVLPKKLDDD
                     GFAFRFPELRSALTNLIR"
     gene            complement(4552..>6216)
                     /locus_tag="PLP95_03395"
     CDS             complement(4552..>6216)
                     /locus_tag="PLP95_03395"
                     /inference="COORDINATES: protein motif:HMM:NF037085.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBG domain-containing protein"
                     /protein_id="HPB44876.1"
                     /translation="DGVITITPAPLTIQASSGNLIPTDPAPVITPLYSGLRAGDTAPA
                     TAPACSTTATTSSPYGTYPTTCVGAADANYTITTIDGTMQVAAARLTVQASSAAMTQG
                     DPLPLVTAAYSGWVDGDTAIDVGATCRPLVAANPPAGQFPTGCSGASDSKYAFTYVDG
                     TIDVAAAGLISYQNAVAVTSVAPASNGVTLPSSSISVFDRSPGTGFGDFTNLTIATTS
                     GLQIVSCTGGNPTTFTGCTGGTGTLHTGAAVTTAAPTDFALSSIIPGGKTGFHPTSVE
                     VVSDVAQQLRLVPTSVVRRGDDSYVRYLQSSWPFGSGNMTIGYCRTESVYSPASSDCA
                     TVRVSVLAPQQVTMGARVSVAGITQNQYQVLNIGSRAATVAKPGDIVGLQVAAASSQI
                     PNEQKAQDVTATVRNVRTFMSVIPIPAGFELIDAKIVGGDERTSTGSVVAVCTQDGPD
                     CTARLTGNWNQTKFPYVQASIGSTVVPGGTTITLPALDLTLRATGAAGTRGTTGLSQF
                     RNVVIVDVPVVGSTTITFDSWPSSGSNTGVTPPVAPMTPLMDTVIN"
BASE COUNT         1016 a         1906 c         2179 g         1115 t
ORIGIN      
        1 caagcccgcc ctggacgccc tgcaccgcta cgcctggccg ggcaacgtcc gcgaactgca
       61 gaacctcctc gagcgggcgc ttgtattgtc cgacggcaag atgatctccc ccgcggaact
      121 ggatctcgag gcttcggccg cagatcagga aaacggcaag ggaacgctga aggaggcgcg
      181 gcaggaagtc gagcgcgaca ccatcgcgag ggcgttccac cggatgaagg cgcggatgcg
      241 acgttcgagg tcggtgtcgt ccgggaacca cgcctgttcg ttcgggggga tcgtgttgat
      301 gtacggcgtc gtgacgagcc ccgagttgtc gatacccatc tcctcggcgc gcatctccat
      361 gcggccgatc aggaagctgg ctcggggttt gccgcgggtg gcggcgaccg attcgagcga
      421 atcgatccac tcggcggtct cctgcgggtc gacgtcgggg agctgttgga ggaagccttc
      481 tgcgctcatc gttcgtacgt cctttgtttg ctggagaagt gatctgcctc cgagtctacg
      541 aactagggtc gagcgccgat ggtgcaggtc gtgtacaccg atggcgcgtg ccgcgggaac
      601 cccgggcccg gtggctgggc gtgggcgctc gtggaggagg gtcgtggcgc cgggctaagc
      661 gaccggtggg cgagcggggc cgaggcccac accaccaatc agcgcatgga ggtcatggcg
      721 gcccaccagg cggtgttggc ggttgacggg cccatcgagg tgcgcagcga ttcaacctat
      781 gtcgtgaaat gcttcaacga ccggtggtat cggggctggc tggcgcgagg ttggctcaac
      841 agtcagaaac aggcggtggc gaaccgcgac ctctgggagc cgttcatcga cgtggtgctc
      901 gcccgtggcg atgtcacctt cacctgggtg aagggtcacg gaaccgatcg gatgaaccag
      961 ttcgtcgatc ggctcgcggt ggaggcgtcg cagcgtcaac gcggggccgt cggtggtgaa
     1021 gcaggtcggg cgctcgccgg cgagtcggct gcgctgttgt tgggcggccg cgcgactggt
     1081 gaacctggag cgatcgtcgc ccaggaatcg ctcttctaga cctcgcgcgg agagacctca
     1141 aacgtccaag ccgttccacg gatccggtgt agaacgctcc ggtttcagta ccaggccggt
     1201 ttcagtacca ggattggggc gcttcggtgc gtcgtgcggt ctgcaggagc gtgtacgcgc
     1261 agttagcttc ggtggtgcgg tcgacggcca tcgccatctc ctcggcggaa cgttccacaa
     1321 agcggtcggg gtggtagcgc ttcacgatgg ctcgatgtgc ggacacgacc gttcgccaac
     1381 ccgcgtcgga gcggaagtcg gtgagcggtt cgatccccag gtcgtagcac gcccggcact
     1441 cgggactcat gatgatttcg ctgtgacccg tcgtggccat gattgccttt cacttcgatc
     1501 ttgagtgtgc cttcacattc ttcggacagt cctagcgaaa agtaaagctt ttcccggctt
     1561 tcttgaccaa gtggtcaacc cgtagaatcc gggcatggat atcaccggat catcagcaat
     1621 cgtcaccggc ggcgcttcgg gcctcggtga ggcaacctcc cgactgcttg ccgaaagggg
     1681 agtcaaggtc gtcgtcgccg acctcgaccg tcaacaggac cggggcgacg ccctggccaa
     1741 cgagatcggc ggactgttcg tcgcctgcga cgtcaccgat gccgagcagg tccaggccgc
     1801 ggtcgatgcc gcggtcgaaa tggcgccgtt gaagtcgctc gtcaactgtg ccggcatcgg
     1861 ctgggcgagc cgcacgatcg gcaagaacgg tgagccgcac gatctggaga tcttccgcaa
     1921 ggtcatcgac atcaacctcg tcggctcgtt caactgcatc cgtctcgccg cgtcggcgat
     1981 gaaccgcaac gagggcgatg ccgacggtgc gaagggcgcc atcgtcaaca ccgcgtcggt
     2041 cgccgcgtac gacggtcaga tcggccaggc cgcctactcc gcatccaagg gcggcgtcgt
     2101 gggcatgacg ctcaccatcg ctcgcgacct tgcggccgcg gggatccgcg tgaacacgat
     2161 cgctcccggc ctgatcgaca ccccgatcta cggcgagggc gaggccgccg aggcgttcaa
     2221 ggaggcgctc ggcaagtccg tgctgttccc ccaccgcctc ggtgtcagcg ccgagttcgc
     2281 gtcgctcgca ctcgaactgc tcacgaactc ctacatgaac gcccaggaca ttcgtctcga
     2341 cggcggcatc cggatgcctc cgaaggcctg agcttcgcga cgacccctgt cggggtccga
     2401 ctgaacctgg gcgtatcgac ggtcacctac gaccgtcgat acgcccaggt tcgcgttttg
     2461 cggcggtgtc tacttggtcg ctcggcggcc aacccaggtc gggtgtgttg ccatcgccaa
     2521 caactcgacg tctccggagg agtctccgta ggcccacacc tcggggtcga tgagacccgc
     2581 ggcgtctatc cacgcggaga gccgggcggc cttttcggcc ttgcggacgt tgttgccgac
     2641 gagatcgccg gtgagtcggc cgtcgtctcc aaccgccatg tccacgccga tcacatcgtc
     2701 gaagccaaga tcctcggcga tcgggcgcag gtagtagacg agcgacgcgc tgacgatgac
     2761 ctgacggtgc ccctgggctc gatgccaggc gatgcgttcg agcgactcgc gtcgaaaccg
     2821 gcgcggcagt gttctcgcat actgaagccc agcggcttcg acctcgtcga ggtggcgccc
     2881 tcgcaaaacg tcggacagga ccagttcctt ggcgcggtcg cgttgggtgc tggatcgcag
     2941 cccccgtgcc agcacggggg catgggaagc gagcgatttt gcgactgcgg atcgtccggc
     3001 gatgaaggtg agaaacggaa cgagggtgtc gcgcgtcgtg atcgtctcgt cgaaatccca
     3061 cgccgcgagc ccggaccgtg gaaagtgctg gatgctcatc gactcagcgc gctcgtcggc
     3121 tcagagcgcg ctcgatcgat tggtgacgta ggtgtcgagg ttctccagcg tcttggccat
     3181 gttgaccttg tggcgcttcg gccacccgag cccctcgatg tagaacttga tcggcgaggg
     3241 tcgccaatcg aaggtctcgg tgacgagcgt gcggggcgtt ccgtcgacat cgtcgagcgg
     3301 ttcgaaggtg tagcgccaga tgtgcttgcc agggtgttgc caggcgatga gacgatcctg
     3361 ttcaaactcg acgacggtgc tggtcagccg gtagggaagg aacatcttca tgttcatccc
     3421 aaacttggac cccagcttca gcggttcgct cttgccggtg gcggaacgaa cggttcccga
     3481 gccatcgatc accgggtgga acgaggcatc cgcgacgatc ttgaacagtt ctgctggcgc
     3541 tgcggcgatc tcacgtgatg ccgaaacctg cttggcgtcg tcggtcatgg tcgctccgtt
     3601 tcggattctc gggggttaac gaatgagatt ggtgagtgcg cttcgcagtt cgggaaaccg
     3661 gaaggcgaag ccgtcgtcgt ccagtttctt gggaagcact cgttgacttt cgagcaggag
     3721 cgtatccgcc aactccggac cgaacagcag cctggggccg aactggggga tcacgccgca
     3781 tgggcggcgc agcaccgacg cgagcgtcga ggcgaactcg gcgttggtga ccgggttcgg
     3841 agcggtgagg ttgaccggtc cggccacggg gcgttcgagc agccagacga tggcccggac
     3901 gtgatcgtcg aggtgaatcc agctctgaaa ttgccggccg tttccgctgc gcccaccgag
     3961 gccgagtttg aagggcaaca tctgttgttt gagggcaccg ccctcggccg acaacacgat
     4021 gccggtgcgt aacaaggccg tggagatccc gagttcaacc gtcggtgcgg cggcggcttc
     4081 ccagcgccga accacgtcgg ccaggaaccc ggacccggcc ggcgagttct cggtgaggac
     4141 ttgagcgcca caatcgccgt agtagccgat cgccgacccc gacaggaacc tcgtcggcgg
     4201 tcgagaggag cgcgccatgg cgtcgaccaa caactcggtc gaacgtacgc ggctgtcgac
     4261 gatgcgtcgc ttctgctcgg tggtccagcg cttggcgccg atcggttcgc cggcgagatt
     4321 gaccaccgcg tcgaagccgt cgatcgctcc gtcggcgagt acgccccggt cggggtccca
     4381 cacgatcgtg tcgacaccgg gtcgacgcga gatcgtcacc gcctcgtgcc cgacggaacg
     4441 gagttcggcg acgagacggc tgccgatcag acccgtggca ccgctgacag cgactcgcat
     4501 ggtgtcgtga tggccgtgtc gtgatggctg tgtcatgacg aggggcgacg atcagttgat
     4561 gacggtgtcc atgagcggcg tcatgggggc caccggtggg gtcacacccg tgttcgaccc
     4621 gctgctcggc cacgagtcga acgtgatcgt ggtcgaaccg acgacgggaa cgtcgacgat
     4681 gacgacgttt cggaactggc tgagtccggt ggtgccccga gtcccggccg cgccggtggc
     4741 cctcagggtc agatcgagcg ctgggagggt gatcgttgtg cccccgggaa cgaccgtgga
     4801 accgatgctc gcctgcacgt aggggaattt cgtctggttc cagttgccgg tgagtcgggc
     4861 cgtgcaatcg ggcccgtcct gggtgcagac cgccacgacc gacccggtcg acgtgcgttc
     4921 gtcaccgccg acgatcttcg cgtcgatcaa ctcgaacccg gcggggatcg ggatcaccga
     4981 catgaaggtc cgcacgttgc gcaccgtggc ggtgacgtcc tgggcctttt gctcgttcgg
     5041 aatctgtgag gatgcggcgg ccacctgcag accgacgatg tcgccgggct tggcgaccgt
     5101 cgcggcccgt gatccgatgt tgaggacctg atactggttt tgggtgatgc cggcgaccga
     5161 gaccctcgct cccatcgtca cctgttgggg cgccaacacc gacactcgca cggtcgcaca
     5221 gtcgctgctg gcgggtgagt agaccgactc ggtgcggcag tagccgatcg tcatgttgcc
     5281 ggaaccgaat ggccagctcg attgaaggta gcgaacgtag ctgtcgtcgc cgcggcgcac
     5341 gacgctggtc ggcaccaacc gcagctgctg ggcgacgtcg ctcaccacct cgaccgaggt
     5401 cgggtggaag ccggtctttc cacccgggat gatgctggac agggcgaaat cggttggggc
     5461 ggcggtggtg accgccgcgc cggtgtgcag cgtaccggtc cctccggtgc agccggtgaa
     5521 ggtggtgggg ttgcctcccg tgcacgacac gatctgcagg ccggaggtgg tggcgatggt
     5581 gaggttggtg aagtcgccga agcccgttcc ggggctgcgg tcgaacaccg aaatcgacga
     5641 ggagggaagg gtcaccccgt tcgaggcggg ggcgacgctg gtgaccgcga cggcgttctg
     5701 gtagctgatg agcccggcgg cggcgacatc gatcgtgccg tcgacgtagg tgaacgcgta
     5761 tttcgagtcg ctcgctccac tgcagcccgt ggggaactga ccggcgggcg ggttcgctgc
     5821 gacgagtggt cgacaggtgg caccgacgtc gatggcggtg tcgccgtcga cccacccgct
     5881 gtaggccgcg gtcaccagcg ggagcgggtc gccctgggtc atcgctgccg acgatgcctg
     5941 caccgtcaac cgcgctgcgg cgacctgcat cgttccgtcg atcgtggtga tcgtgtagtt
     6001 ggcatcggcc gcaccgacgc aggtcgtggg gtacgtcccg tacggcgagg aggtcgtcgc
     6061 cgtcgtcgaa cacgccggtg ccgtggccgg tgcggtgtcg ccggcgcgaa ggccgctgta
     6121 gagcggggtg atgacgggcg ccgggtcggt cgggatcagg ttgccgctcg aggcctgaat
     6181 cgtcagtggg gccggcgtga tggtgatgac cccatc
//
LOCUS       DAOOMA010000076         6048 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_15587_length_6048_cov_3.509928, whole genome shotgun sequence.
ACCESSION   DAOOMA010000076 DAOOMA010000000
VERSION     DAOOMA010000076.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 6048)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6048)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6048
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15587_length_6048_cov_3.509928"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..548
                     /locus_tag="PLP95_03400"
     CDS             <1..548
                     /locus_tag="PLP95_03400"
                     /inference="COORDINATES: protein motif:HMM:NF021509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="oxygenase MpaB family protein"
                     /protein_id="HPB44877.1"
                     /translation="ETFWWAHATFQWAVFQLIDRFDNHRLTDAERESLYLDGIEWYRR
                     YGMGMDPVPPTYAEFVDKWEYYCREVLELNVASSRSLDMALHEKVSDMPGLPGWSNPL
                     QREVLTPLLRLTAIGGLPRTVRKRFDLPWGRHEELQMKGLELWVRQNWRFVPRHLRYA
                     PTALAGKDRVRNERRSLAHAS"
     gene            538..1176
                     /locus_tag="PLP95_03405"
     CDS             538..1176
                     /locus_tag="PLP95_03405"
                     /inference="COORDINATES: protein motif:HMM:NF037271.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HPB44878.1"
                     /translation="MRRSLTVDADGVVASLANGPVDDDADDTTTRILDAAAELYATIG
                     PRRCSVEDIAERSGLGRTTIYRRFDGRQQILEAVIGREVRRFFASILAPTAGLHRFND
                     VVVESFVHGLASSETSLLAAMVRSEPELLALLTIDGGPIIDAAREFLLDLTAQAGVGS
                     DADTTATLVEVLVRLAISLVLTPSATFPLDDPDTARAALHRVLDPILAALAP"
     gene            1279..1950
                     /locus_tag="PLP95_03410"
     CDS             1279..1950
                     /locus_tag="PLP95_03410"
                     /inference="COORDINATES: protein motif:HMM:NF024922.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoglutarate-dependent dioxygenase AlkB"
                     /protein_id="HPB44879.1"
                     /translation="MTNRAPLTIDRHAVVERLWLDETSWVDVVRRFVREPATLTASLL
                     DDVVWRPDEQFRYEVTITSPRLIATPRGESLPAAVRQIELHLAATYRRRFSGPALIQY
                     RTGRDSVAFHRDREMRWLDDTLIAIVSLGATRPFAFRPVHSRRDDDTHDHRIDLNSGD
                     LIVMGGRAQQDWLHAVPKCDASEPRVSMTWRWTSRGGRPDSSAPYRGARHFGDSGRVG
                     PQRRN"
     gene            1955..2467
                     /locus_tag="PLP95_03415"
     CDS             1955..2467
                     /locus_tag="PLP95_03415"
                     /inference="COORDINATES: protein motif:HMM:NF024966.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HPB44880.1"
                     /translation="MQNSSPTDGSRSDLDPDLAETVAYCAIERLQRRYGDAVTRQAWD
                     EVAACFDPEATVTVDLIDRDPLRFDGGNSIASFIAASVERFSFFEFSLLNSVVFDIDA
                     AHRTASGRVYIMEIRQLAETGRWSHAYGLYRDEYRHTDGQWRHSSRHYSSLARRFDDG
                     TSEVFAIPRQ"
     gene            complement(2531..4037)
                     /locus_tag="PLP95_03420"
                     /pseudo
     CDS             complement(2531..4037)
                     /locus_tag="PLP95_03420"
                     /inference="COORDINATES: protein motif:HMM:NF017591.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PhoX family phosphatase"
     gene            4532..5758
                     /locus_tag="PLP95_03425"
     CDS             4532..5758
                     /locus_tag="PLP95_03425"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR02032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HPB44881.1"
                     /translation="MTSVSHTRDLLVIGGGPGGSAAAIRAARAGAKVTLFEKGAPGRD
                     KICGDGLTPRAIRALLELDIDLTGIHRTRGLRMKAFGNARELDWPGGAFPDWGAVWPR
                     RRLDTHLVEVAEEAGAEVLWSHVATPIMDGNRVVGVEANGGRWHAPMTIVATGAPNAV
                     TKGLGADRNPDEPFGLAIRTYAATPRDTDAFLEADLTLRDDNGAPIPGYGWMFPVGDG
                     TVNIGVGALSTMKGFKKLNLNKLQDIYRASVAEEWELGPDLERPRAWRLPMSVTKTHG
                     PGWVVVGDAAGLVNPMNGEGIDYGLESGILAADLALDDPLYAPARYARGVRARYEPFM
                     DMGRRFSFLVGHPWILRTGLRFAVSTDAISKITLEVMANLVEADSPGASGWTYRTAGR
                     LFDVADPLLRATRAER"
     gene            complement(5817..>6048)
                     /locus_tag="PLP95_03430"
     CDS             complement(5817..>6048)
                     /locus_tag="PLP95_03430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB44882.1"
                     /translation="DVRFDDFMADQWGILEAIYQVADQPRSPETVAAQRAYLDSHGRA
                     RHGSVIYEADTLGIDPDALRGSFADYTARFLA"
BASE COUNT          976 a         2047 c         1991 g         1034 t
ORIGIN      
        1 ccgagacctt ctggtgggca catgccacct tccaatgggc ggtgttccaa ctcatagacc
       61 gattcgacaa ccaccgcctc accgacgccg aacgagagtc gctctacctc gatggcatcg
      121 agtggtaccg gcgctacggc atgggcatgg acccggtgcc gccgacctat gccgagttcg
      181 tggacaagtg ggagtactac tgccgcgagg tcctcgagtt gaacgtggcc tcgagtcgtt
      241 ccctcgacat ggcccttcac gaaaaggtgt ccgatatgcc cggactaccc ggctggagca
      301 acccgttaca acgcgaggtt ctgacaccgc tgcttcgcct gacggccatc ggcggactcc
      361 ctcgtaccgt ccgcaaacga ttcgacctgc cgtggggtcg ccacgaggaa ctccagatga
      421 aggggcttga gctgtgggtg cgccagaact ggcgcttcgt cccccgacat ctccggtacg
      481 ccccgaccgc gttggcgggc aaggatcgcg tccggaacga acgtcggagt ctggcccatg
      541 cgtcgtagcc tcacggtcga cgcggacggg gtcgtcgctt ccctcgccaa cggcccggtc
      601 gacgacgatg cggacgacac gacgacgcgc atcctcgacg ccgccgccga gctgtacgcc
      661 accatcgggc cgcgccggtg cagcgtcgag gacatcgccg aacgcagcgg gctcgggcgc
      721 accaccatct atcgccgttt cgatgggcgc cagcagatcc tcgaggcggt catcggccgc
      781 gaggttcgcc ggttcttcgc atccatcctc gccccgacgg cgggcttgca tcgcttcaac
      841 gacgtcgtgg tcgagtcgtt cgtacacggc ctcgcatcga gcgagacgtc gttgctcgcc
      901 gcgatggttc gctccgagcc ggagctcttg gcactgttga ccatcgacgg gggcccgatc
      961 atcgacgccg cccgcgagtt cctcctcgac ctcaccgctc aggcgggcgt cggcagcgac
     1021 gccgacacga ccgccacgct cgtcgaggtg ctcgtgcgcc tggccatctc gctggtgctc
     1081 acgccatccg cgaccttccc cctcgacgac cctgacaccg cccgcgccgc gctgcaccgg
     1141 gtgctcgatc cgatcctcgc cgcgctcgcc ccctgaccgc tcccgcgccc acccacgtcc
     1201 caaccacgcg ttctgtaggt aatcgcggcg gctatggcca ccgcgattac ctacagaact
     1261 ggcaaggtag gggctgcgat gaccaaccgg gcgcctctca ccatcgaccg ccacgctgtg
     1321 gtcgagcgtc tatggctcga cgagaccagt tgggtcgacg tcgtgcgccg cttcgtgcgc
     1381 gaaccagcga ccctcaccgc ctcgctgctc gacgacgtcg tgtggcgccc cgacgaacag
     1441 ttccgctacg aggtcacgat cacctcgcca cgcctcatcg ccacccctcg cggcgaatcg
     1501 cttcccgctg cggtgcgcca gatcgaactg caccttgcgg cgacgtatcg gcggcgattc
     1561 agcggcccgg cgttgatcca gtatcgaacc ggccgcgact cggtcgcctt tcaccgcgac
     1621 cgcgagatgc gctggttgga cgacaccctc atcgccatcg tgtccctcgg agccacccga
     1681 ccgttcgcct tccggccggt ccattcccgc cgcgacgacg acacacacga ccaccgcatc
     1741 gacctcaact ccggcgacct catcgtgatg ggaggtcggg cccaacagga ctggctccac
     1801 gccgttccaa agtgcgacgc aagcgagccg cgggtctcga tgacctggcg ttggacctcg
     1861 cggggcggtc gacccgactc gagcgcgccg tatcggggtg cccggcactt cggcgattcg
     1921 ggcagggtcg gcccgcagcg acgaaactga gccgatgcag aacagctccc ccaccgacgg
     1981 ctcccgcagc gacctcgacc ccgacctcgc cgaaaccgtt gcgtactgcg cgatcgagcg
     2041 gctgcaacgc cgctacggcg acgccgtcac ccgccaggca tgggacgagg tcgctgcctg
     2101 cttcgacccc gaggccaccg tgacggtcga cctcatcgat cgcgatcccc tgcgcttcga
     2161 cggaggcaac tcgatcgcga gcttcatcgc ggcatcggtc gagcgcttct cgttctttga
     2221 gttctcactg ttgaactcgg tcgtgttcga catcgacgcg gctcaccgaa ccgcgagcgg
     2281 gcgggtctac atcatggaga tccgccaact cgccgaaacc ggccgatggt ctcacgcata
     2341 cggcttgtat cgcgacgagt accgccacac agatggccag tggcggcact cgtcgcgtca
     2401 ctattcatcg ctcgctcgcc gcttcgacga cggcaccagc gaggtgttcg caattccccg
     2461 gcagtaacga ttcctgccgt ggccgatcag gagccgatca ggaaccgatc aggaaccgat
     2521 caggaaccga tcaggagccg atctttccgc cgccgatctt gcggatcaca accgtcgccg
     2581 ggcgcggccg gccgtcgggg tcgtagtccg gccagttgct caccgctcgt gggttggcgt
     2641 tggtcggtga accgaatgcc ggagtcgact cacccggatg ctgaatgttc acgaacatcg
     2701 tctgctggtc cggggtgatg gcgatgccgg tcagttcgca gccgcgcggg ccgacgagga
     2761 agcgacgggt ctcaccactg accgggtccg cgcacagcat ctggttgttg ccgatgttgt
     2821 cgtaggcctt cgacaccagg ttctgcgacg agttcgagat gtcggtctgg atccagatgc
     2881 ggccgtcgtc gtcgaccatg atgccgtcgg gcgacccgta gatgtcaccc acgatgtttc
     2941 cggccgctga caacgccggg taggcggggt cgccacacag cgcgaagatg ttccagtcga
     3001 aggtgaggga gcggaagtca tcgccggtct cgttccagct catgatcgag ccgtacggat
     3061 tggtgccacg tggattcgca gcgttctggt ttcctgaccc gttggtgagt gtgcagaaca
     3121 cggtgttcga caccgggctc accgcaagcc attccggacg gtgcatcggc gtcgcccgtg
     3181 ccgtcgtcgt tgaaacgcgc cacatacagc gttccttcgt cgagcgggct gcggccggcg
     3241 gcgatgagtt cctgccacgg ggcggacccg acgaacttgt agatgtattc gctgttctca
     3301 tcgtcacccg agtagaacac ggcgactccg gccgtctcgg cgtaggtcgc accctcatgc
     3361 ttgaaccgac cgagcgcggt gcgcttcacc ggcgtggagt tcgggtcaaa cggatcaatc
     3421 tccacgatcc agccgaagcg gtttgtctcg tcagggttcg cggcgagatc gaaacgcggg
     3481 ttcgactcgt gccacttgag cgacgagttc ttggtcaccc cgtgacgagc ctgatcgtcg
     3541 ctcagggtgt accccgacga cgctgtgccg aagtactggt tccagttctc ctcgcacgtg
     3601 aggtaggtgc cccagggcgt gaacccgttg gcgcagttgt tcaacgttcc ctcgggcggc
     3661 agcgaccctg cgacggcggg gtgcgacgca tcgactggac ccgagaagat gacgggggtc
     3721 gacccggtga tgcgacggtt gtaggacgaa tcgaccaggc gccacttgtt ggcgcgaagc
     3781 tcgaactgca cgaccgagac cccgtgggcc gccagtgcct ttgcgacctg ctcggccgac
     3841 ggcgaaccgg ctgacgccgg gtagatctgc gtggtgtcga cgtactcgtg gttcagcacg
     3901 agcaggccct tgcggcttgc ttgcgctccc tcgccgagcg cgaagtagtg catcccgtcg
     3961 tggttgaagc cgatctgttg agcctggtcg gcggcggtgt tcgaggcgtc ggtacgaaac
     4021 gcggggcccg aactcaagat ggggggtgcc ccacgggatc agcacctcgg cggcgtaccc
     4081 gtcggcaacg gtgatccggt cggccttgtt ggccgcgacg ctttcaaatc caaggagtgg
     4141 accggccggg accgtcgtgg tggtggtcgt cgtggtcgag cctggtgact ccggcgagca
     4201 tgcggtcagc gcgccgattc ccgcaagcga cgcggcaccg agtccgccgc cgatgagact
     4261 ccggcgagag aatctcgtcg cgatgacctc gtcgatcgga cgattggcgg atgggttcga
     4321 gggaggtgga acgggtcaca gctggtctcc tcactgcgtt ggggcgtcga catgtggtgg
     4381 tgtcgatcgg caacctacgg agccaacctg tgcgtgaagc gacctggggg tgaactcggg
     4441 gacaagcctt cgtggatttc cgtcgaccag cggcgccggt gccgcggcgc ggactggtcg
     4501 ccggggtcac gaagctgtca gggttgaggc cgtgacgtct gtgagccaca cccgcgacct
     4561 gctcgtcatc ggcggaggcc ccggtggatc ggcagcagca atccgcgccg cacgagcggg
     4621 cgcgaaggtc acgctgttcg aaaagggcgc tccggggcgc gacaagatct gcggcgacgg
     4681 cctgacgccc cgcgccattc gagcgctgct cgaactcgac atcgacctga ccggaatcca
     4741 tcgcacccgt ggccttcgga tgaaggcgtt cggcaacgcc cgtgagctcg actggcctgg
     4801 gggcgccttc cccgactggg gggccgtgtg gccgcggcgt cgtctcgaca cccacctggt
     4861 cgaggttgct gaagaagccg gggccgaggt gttgtggagt cacgtggcga ccccgatcat
     4921 ggacgggaac cgggtggtcg gggtcgaggc caacggtggt cgctggcatg ccccgatgac
     4981 gatcgtggcg acgggagcac cgaacgccgt gaccaagggt ttgggggccg atcgcaatcc
     5041 cgacgaaccc ttcggcctgg cgattcgcac ctacgcggcg acgcctcgcg acaccgacgc
     5101 gttcctcgaa gccgacctga ccctgcgcga cgacaacggc gccccgatcc cgggctacgg
     5161 ctggatgttc ccggtcggcg acggcaccgt gaacatcggt gtgggtgccc tgtcgaccat
     5221 gaaggggttc aagaagctga acctcaacaa gctccaggac atctaccgcg ccagcgtcgc
     5281 cgaggagtgg gaactcggac cggatctcga acggccccgc gcctggcggc ttccgatgag
     5341 cgtcacgaag acgcacggtc cagggtgggt cgtggtcggc gacgcggctg gcctggtcaa
     5401 cccgatgaac ggcgagggaa tcgactacgg cctcgaatcg ggaatcctcg ccgcggatct
     5461 ggccctcgac gacccgctgt atgcccccgc gcggtacgcc cgcggggtgc gtgcgcgcta
     5521 cgagccgttc atggacatgg gtcgtcgctt ctcgttcctg gtggggcacc cgtggatcct
     5581 gcgcaccggc cttcgtttcg ccgtgtcgac cgatgccatc tccaagatca ccctcgaggt
     5641 gatggcgaat ctggtcgagg ccgactcacc gggtgcgagc ggctggacct accggaccgc
     5701 gggccggttg ttcgacgtcg ccgacccgct acttcgcgcc acccgagctg aacgctgaac
     5761 gccacgctgc gccagcgttg cggcacgccg ctgcggcctt gcagcgctgg cgggaactac
     5821 gcgagaaacc tggccgtgta gtcggcgaag ctgcctcgca gcgcgtcggg gtcgatcccc
     5881 agcgtgtccg cctcgtagat caccgaaccg tgccgtgccc gcccgtggct gtcgaggtag
     5941 gcgcgctgcg cggcgaccgt ctcgggcgag cgaggctgat cggcgacctg gtagatcgcc
     6001 tcgaggatgc cccactggtc ggccatgaag tcgtcgaaac gcacgtcg
//
LOCUS       DAOOMA010000077         6045 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_15598_length_6045_cov_3.867446, whole genome shotgun sequence.
ACCESSION   DAOOMA010000077 DAOOMA010000000
VERSION     DAOOMA010000077.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 6045)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6045)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6045
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15598_length_6045_cov_3.867446"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..749)
                     /locus_tag="PLP95_03435"
     CDS             complement(<1..749)
                     /locus_tag="PLP95_03435"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HPB44883.1"
                     /translation="MSEDDCREPLVELRHVTLGYDREPVVRGIDLSICRGDVVAVLGS
                     NGSGKSTLVKGMLGLSELLDGEILLFGTPARRFSQRCRIGFVPQRNQVASNIPTTALE
                     VVESGRVARRGLWRPFTRADRDAARAALRTVGLDHVASRPVQHLSGGQQRRVAIARAL
                     AGEPELLILDEPTAGVDAETQLRLAATLQQLAEAGTTIVLIEHELGVVADLIGRVLVV
                     ADGSISYDGAPDAAHTHHADHEHHDHTSEPLR"
     gene            complement(746..1762)
                     /locus_tag="PLP95_03440"
     CDS             complement(746..1762)
                     /locus_tag="PLP95_03440"
                     /inference="COORDINATES: protein motif:HMM:NF013465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HPB44884.1"
                     /translation="MPSTALPRRPARVAAVVLLGTALIGSAACSDTSANRPASEAGAI
                     EVVAAFYAAETLAREVGGDRVNVRLLGSTGADPHHIELTPDDVIAVADAQIVVAMRGF
                     QPAVDEALAEANDDAIVDLATVIDLRSSEPHEAQHEEGDHEAADHDEAEHDEADQDEG
                     DHDHGPIDFHFWLDPTLMSDGAIAVRDALVAASPDHREEFDTNLATFQSELGELDTGY
                     RDGLATCERGEIVTTHRAFGYLADRYDFIEDSITGLTPEDEPSPAALERLADHITDSG
                     ITTVFFETLANPAIARTLAEETGATTAVLDPIEGVSEASPGTDYVSIMTANLAAIRTA
                     RGCQ"
     gene            complement(1811..3193)
                     /locus_tag="PLP95_03445"
     CDS             complement(1811..3193)
                     /locus_tag="PLP95_03445"
                     /inference="COORDINATES: protein motif:HMM:NF014244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M50 family metallopeptidase"
                     /protein_id="HPB44885.1"
                     /translation="MARPLFTVAGIPVRIDPAFIFGALLIYLWSGGGTHGIYAVVALA
                     IFLFIHEMGHAVVARHFGAKAGITVGFLGGYTSYVPPATITRGQQIAISAAGSATQLI
                     VALPALYATLLWTRSVAQSADFTSVATFSALETPANVYAAVAWAGVLLALLNLIPALP
                     FDGGRIVERLLRGRFGDATSTIMARWSYGVAAVLALMYVISGGSERNSPLYLTPPQMD
                     LFDPSFVTMMAREGVRFVNFLTSGSLFVPLIIVLGAAAEGRARAVVTPKIGRPGEAPP
                     LDAQSLLAAVESAERSGWATGTVPPFPAGFEPSPWLVTYAAQRRGDTTTAQQAPHLLS
                     DVRERRWVRLPDPNHPAILDLLQHLPPDVADSAAVLEMRVHAGSVDDFASAIQRRVTA
                     TGAPSDLYLGAAGFATRGYNDSALAWLERAVSAEPSATLLATAPEFGSLRGDPRFEQL
                     LERAQVAEGV"
     gene            3939..4298
                     /locus_tag="PLP95_03450"
     CDS             3939..4298
                     /locus_tag="PLP95_03450"
                     /inference="COORDINATES: protein motif:HMM:NF024139.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HPB44886.1"
                     /translation="MSTPDLLTITEAAVVLRIGRTTAYELARRDLATGGGEGLGVIRV
                     GGQLRVPRVALERLIGAPIVLSVETGAVDGSSGSVAAAVLPAAIPNPTPARVDHTSDG
                     PGRCRRLVSMDQLRFDS"
     gene            complement(4795..5223)
                     /locus_tag="PLP95_03455"
     CDS             complement(4795..5223)
                     /locus_tag="PLP95_03455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB44887.1"
                     /translation="MVLHPSGGVRRELCGASSVEPVFRHVSFDLVASCGELVDDLHRD
                     RRQIRDPASHRPPVDTQRGGELVAELGLVEVADGLGPVVQRPRIERCPPVVGPMNQVR
                     DHHVSVEMRVTGTRRAMPERGGDEPVTGNYLSAAVATPTP"
     gene            5363..>6045
                     /locus_tag="PLP95_03460"
     CDS             5363..>6045
                     /locus_tag="PLP95_03460"
                     /inference="COORDINATES: protein motif:HMM:NF024991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HPB44888.1"
                     /translation="MFEHLAGPDGLTRNRSTFGRREVIQGICDLLPNGAPVADIGQWA
                     DLFIESGHCTRLAGPKSAVIRTNDGRTVSARTDETRFSTPGMLATEQRLIASAVDRVE
                     AGVGIADPHHVGIAIAQRPSMSIEQADILTRICGSGRGVDLVEGVAGAGKTFALAAAN
                     QAWTDSGHTVIGCALAARAARQLQTDADIPSSTIDRFLIDLDRPERGGLAANTVIVVD
                     EAAMVGTRKV"
BASE COUNT         1042 a         1991 c         2058 g          954 t
ORIGIN      
        1 cgaaggggtt cggaggtgtg atcgtggtgt tcgtggtcgg cgtgatgggt gtgggccgcg
       61 tcgggggcgc cgtcgtagga gatcgagccg tcggccacga cgaggacccg gccgatcagg
      121 tcggccacga ccccgagttc gtgttcgatg agcacgatgg tggtgcccgc ctcggcgagt
      181 tgttgcaagg tggcggcgag gcgcaactgg gtttcggcgt cgactccggc ggtgggctcg
      241 tcgaggatga gtagctcggg ttcgccggcc agcgcacggg cgatcgccac ccgacgctgc
      301 tgtccacccg acaggtgttg caccggtcgg gacgcgacgt gatcgagccc gaccgtgcgc
      361 agcgcagcgc gggcggcatc gcggtcggcg cgggtgaagg gtcgccacag cccgcggcgg
      421 gcgacccggc cgctttcgac gacctcgagg gcagtggtcg ggatgtttga cgcaacctgg
      481 tttcgctggg gcacaaagcc gatccgacaa cgctgggaaa agcgccgtgc gggggtgccg
      541 aagagcagaa tctcaccgtc gagcagctcg ctcaatccga gcatgccctt gacgagggtc
      601 gacttgccgg agccgttgga accgagcacc gccaccacat cgccgcggca gatcgacaga
      661 tcgatgcctc gcacgaccgg ctcgcggtca tagccgagcg tgacgtggcg taactccacg
      721 agcggttccc ggcagtcgtc ctcgctcact ggcatcccct ggcggtgcgg attgcggcca
      781 ggttggccgt catgatcgaa acgtagtcgg tgccgggcga tgcctcgctc accccctcga
      841 tcgggtcgag tactgcggtg gtggcgccgg tctcctcggc caaggtgcgg gcgatggcgg
      901 ggttggccag ggtctcgaag aagacggtgg tgatgccgct gtcggtgatg tggtcggcga
      961 ggcgctccaa cgcagcgggg gagggttcgt cctcgggggt caacccggtg atcgagtctt
     1021 cgatgaagtc ataacgatcg gcgagatacc cgaacgcccg gtgggtggtg acgatctcgc
     1081 cgcgttcaca ggtcgcgagc ccgtcgcgat acccggtgtc gagttcgccg agttccgact
     1141 gaaaggtcgc caggttggtg tcgaactcct cgcgatggtc ggggctggcg gcgaccagcg
     1201 cgtcgcggac cgcgatggca ccgtcgctca tcaacgtggg atcgagccag aaatggaaat
     1261 cgatcgggcc gtggtcgtga tcgccctcgt cctgatcggc ctcgtcgtgc tcggcctcgt
     1321 cgtgatcggc cgcctcgtga tcgccctcct catgttgggc ctcatgcggc tcggaggatc
     1381 gcaggtcgat cacggtggca aggtcgacga tggcatcgtc gttcgcctcg gccaacgcct
     1441 cgtcgaccgc cggctggaat ccccgcatcg ccaccacaat ctgggcatcg gccacggcga
     1501 tgacatcgtc gggggtcaat tcgatgtggt gcgggtcggc cccggtggat ccgagcaaac
     1561 ggacattgac acgatcgccg cccacctcac gggccaaggt ttccgcggcg tagaacgcgg
     1621 cgacgacttc gatggcaccg gcctcgctgg ccggccggtt ggccgacgtg tcggagcagg
     1681 ccgctgaacc gatcagcgcc gtgcccagca gcacgaccgc ggccacgcga gctggccggc
     1741 ggggaagggc ggtggaaggc atgccaccac catacttaaa atgagaacca ttcgctatat
     1801 atgcgcgacc tcagacgccc tccgccacct gcgcacgttc gaggagttgt tcaaagcggg
     1861 ggtcgccgcg cagcgaacca aactccggag cggtggccag gagggtcgcg ctgggctcgg
     1921 ccgacaccgc ccgctcgagc caggcgagcg ccgaatcgtt gtagccgcga gtggcgaatc
     1981 ccgccgcccc caaataaagg tcgctcgggg cgccggtcgc ggtgacccgg cgctggatgg
     2041 cggaggcgaa gtcgtccacc gacccggcgt ggacccgcat ctcgagcacc gcggcgctgt
     2101 cggcgacatc ggggggcaaa tgctggagca ggtcgaggat cgccggatga ttcggatctg
     2161 gcagcctcac ccaacgccgt tcccggacat cggacagaag gtgcggcgcc tgctgcgcgg
     2221 tggtggtgtc gccgcgacgc tgtgccgcgt aggtcacgag ccacggtgag ggttcgaacc
     2281 ccgcaggaaa gggtggcacc gtgccggtcg cccagccgct tcgctcggcc gactcgaccg
     2341 cggcgagcaa cgactgcgca tccaacggtg gagcctcgcc gggccggccg atcttcggcg
     2401 tcacgaccgc acgagcacgc ccctccgccg ccgcacccaa cacgatgatc aacggcacaa
     2461 acaacgaccc cgaggtcaag aagttcacga accgaacccc ctcgcgagcc atcatcgtga
     2521 caaaggacgg gtcgaacaga tccatctgcg gcggggtgag atacagcggg gagtttcgct
     2581 cggaaccgcc cgaaatgacg tacatcaatg cgagcacggc agcgaccccg taggaccacc
     2641 gagccatgat cgtggaggtg gcatcgccaa aacggccgcg caaaaggcgc tcgacgattc
     2701 ggccaccatc gaagggcaac gccgggatca ggttcagcag tgcgagcaac accccagccc
     2761 aggcgacagc ggcgtagacg ttggccggcg tttcgagcgc actgaaggtc gcgaccgacg
     2821 tgaaatcggc ggattgtgcg accgaccgag tccacaacag ggtggcgtac aacgccggca
     2881 acgccacgat caactgggtg gccgatcccg ccgcgctgat ggcgatctgt tgaccgcggg
     2941 tgatcgtcgc gggcggcaca taactcgtgt agccgccgag aaacccgacg gtgatgcccg
     3001 ccttggcccc gaaatggcgg gcgaccaccg cgtgacccat ctcgtggatg aacaagaaga
     3061 tggccagcgc caccaccgca tagatgccat gggttccgcc gcccgaccac aggtagatca
     3121 gcagggctcc gaagatgaac gcggggtcga ttcgtaccgg gatcccggcg acggtgaaca
     3181 gcggtctcgc catggcgcaa ggctagtgcc gtgccgggcg ccggccgacg gcgcgacacc
     3241 ggcggcggct accgcgcgaa gtttgtgccg aaaccgacac cccgtgtcgc tttcggcaca
     3301 aacttcgcgc gaattgggca ctcgaaaacg acgaggcccc ctccgaagag ggggcctgtg
     3361 ctgagcggac gacgagattc gaacccgcga ccctcacctt ggcaaggtcg aaatcatcgt
     3421 ccacccggtg ccgagaagcc ctacaaactg gggtttcgtc ccactcgcta gccgacggat
     3481 gacatcgaat atcggaaggt agtcgaacgc tctacttcgt ggctggggcc agcctccgat
     3541 tgattgacca ctgacggtca agaccggcga cacgcgccgt cctgggctgt tctaccggaa
     3601 atcagggggc ccgaggttgg cggcacgtca tcctcccaga cgtcgccggc tcgagtaacg
     3661 acggcgaagc ggacagcgaa cacgacggcg aagacgacgg cgaaccggac acctccatcc
     3721 tgaccgccac cctcgggcct gctcctcggc gtcccggtcg gtctgccttg gtcagggatg
     3781 accagcgaga tctcctgacc gatttcgatc tcgcgttcag aggtgtgtcc agggtgacca
     3841 gccattgtcg gaccccggcg cggtcgtgct tcatccgctg agtcggagtc tcgatgcgcc
     3901 tagcgatacc aacagccgac aacgcttgga gtttgccgat gagtacacct gatctgttga
     3961 ccatcacgga ggcggccgtc gtgttgcgca tcggtcggac caccgcctac gagctcgccc
     4021 gacgcgacct tgccaccggc gggggagaag gcttgggggt gatccgtgtc ggagggcagc
     4081 tgcgggtccc acgggtggcg ctggagcgcc tgatcggcgc cccgatcgtg ttgtcggtgg
     4141 aaacgggcgc cgtcgacggc tcctctggga gcgtcgcagc ggcggtcttg cccgctgcca
     4201 taccgaaccc gacaccagct cgtgtcgacc acaccagcga tggccccggc cgctgtcgtc
     4261 ggcttgtgtc gatggatcag ctgaggttcg attcgtgacc agttgcgcgg tcggctggac
     4321 ggtgccttgg tcaggtgcca tttcgttcga cgcacttgca ccacggtgcc ctggcgtcag
     4381 tcacgccggt tgtcaccttt tgtggtcgcc ttgttgagtc tcggcaagct cgctgccggt
     4441 ggcggtgact actacaccgc gatggtcgct gacggcgccg aggagtagta ctacaccacc
     4501 actcgcgaag cacccggcga gtgggtgggt gcggcggcgc agcggttggg attggccggc
     4561 acggtccgac ccgaagactt cgccgctgtg ctcgagcacc gctatccggg aacggacgtc
     4621 agggtcaccg cgtcacggtc cgctccgacc gtggcggcgt ttgatgccac gttcaacgca
     4681 ccgaagtcgg tgtcggtcct ccacggtctc ggcacccccg acatccgagc tgtggtccgg
     4741 ctggcgcacg atgtcgcggt gcgtgaagcg ctcacggtgt tcgagacgga agcgtcacgg
     4801 ggtcggcgtg gccacggcgg cgctgagata gttgccggtg acgggttcgt cgccgccgcg
     4861 ttccggcatc gcacgtcgcg tgccggtgac ccgcatctcc acactcacgt ggtgatcgcg
     4921 aacctggttc atgggcccga cgaccggtgg acagcgctcg atgcgcggcc gttgtaccac
     4981 tggtccaaga ccgtcggcta cctctacgag gccgagctcc gccaccaact cgccaccacg
     5041 ctgggtgtcg actggcgggc ggtgcgacgc gggatcgcgg atctgtcggc ggtcccgatg
     5101 caggtcgtcg acgagttctc cacacgacgc cacgagatcg aagctcacat ggcggaaaac
     5161 gggttcgaca ctgctcgctc cgcacagctc gcggcgtacg ccacccgacg gatgaaggac
     5221 cattcggcga cccccgagtt actcgcacag ggctggcggc aacgggccgc cgagttgggg
     5281 ttcgactgcg agcaggtgtg cgaccgactc aacgccgaca cgctcacgcc ccgatcgacc
     5341 ggactggtcc acctcactcc ggatgttcga gcacctcgcc ggccccgatg ggctaacacg
     5401 gaaccggtcg acgttcggac ggcgcgaggt catccagggc atctgcgacc tcctgccgaa
     5461 cggcgcaccc gtcgccgaca tcggccagtg ggccgacctg ttcatcgagt ccggacattg
     5521 cacccgactc gccggaccca agtccgccgt catccgaacc aacgacggcc gaaccgtctc
     5581 ggcacgaacc gacgagactc gcttctccac gcccggcatg cttgccaccg aacaacgact
     5641 gatcgcctcg gccgtcgacc gcgtggaggc tggcgtcggg atcgccgacc cacaccacgt
     5701 cggtatcgcg atcgcacaac ggccctcgat gagtatcgag caggccgaca tactgacccg
     5761 aatctgtggg agcggccgag gtgtcgacct cgtggagggc gtcgccggcg cgggcaagac
     5821 gttcgcactc gctgccgcca accaggcgtg gaccgactct ggccacaccg tgatcggctg
     5881 tgcgctcgcc gcgagggcgg cacggcaact ccagaccgat gccgacattc cgtcgtccac
     5941 gatcgatcgg tttctcatcg acctcgaccg tcccgaacgg ggcggtctcg ccgcgaacac
     6001 tgtgatcgtg gtggacgagg ccgcaatggt cggcacccgc aaggt
//
LOCUS       DAOOMA010000078         6016 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_15703_length_6016_cov_3.598222, whole genome shotgun sequence.
ACCESSION   DAOOMA010000078 DAOOMA010000000
VERSION     DAOOMA010000078.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 6016)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6016)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6016
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15703_length_6016_cov_3.598222"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..207
                     /locus_tag="PLP95_03465"
     CDS             <1..207
                     /locus_tag="PLP95_03465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB44889.1"
                     /translation="KGVASYRTDAEVNDELLVVDDDEPVAWILGQADESDLELTDEQV
                     AAVLLQQAEYYRRIGAVGPQVATP"
     gene            275..502
                     /locus_tag="PLP95_03470"
     CDS             275..502
                     /locus_tag="PLP95_03470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB44890.1"
                     /translation="MKRLGKFEHNRYVGDKRSQVVYDIDELDDEYRSVIDELMAAETY
                     LCFGPDTLDEARNRGYRLFGPQRRTAESAAN"
     gene            517..1308
                     /locus_tag="PLP95_03475"
     CDS             517..1308
                     /locus_tag="PLP95_03475"
                     /inference="COORDINATES: protein motif:HMM:NF012770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HPB44891.1"
                     /translation="MAGEATMDSVLEVDGLRLSAHIARPTQDRGIPESLLPGVVVCHG
                     FPVRGREAPASGKSFPELADRIAHEMGWLAMTMNFRGCGRSEGDFSLSGWVQDIEAAV
                     THLQDLGASAVWLAGFGSGGALTLCAGARRPDVRGVAAVSAPADFSDWARHPRRLVEH
                     SRSVGVIKTPGYPFDFDRWAGEFRGLTAVSAAERLAPRPLLVMHGEVDDLVPSIDARA
                     IADAHGNAELRIIAGGGHELRHDPRAIAVLLGWLNRQHNAAVADA"
     gene            1387..4161
                     /locus_tag="PLP95_03480"
     CDS             1387..4161
                     /locus_tag="PLP95_03480"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012403.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HPB44892.1"
                     /translation="MTGSTFLSRPGRVHAAFIPAEPPRSSRLALWHRDPEVVDALESL
                     GARGSIDVVIPGDVGVERVEVDAVFLPMAAAIDWLMGSSNEMASTHALARVVRSALGL
                     VARGLLQPSVSDDGTDRWVVSPLTSDDLIVRSLLVTHLPPSAHCIATGVGAERMQAAA
                     EVVHGLIAAVVDSMPRTPGAAVAVDQVAWATGAAVDVSRLRSRLAGTEVHERVTVGLR
                     VIPPLDEGLAFTCEVQVRSTSDPAVISSAAQLWAGEVDWVDRSAELEVLRTLRRGAER
                     WKPLERLLELPEPVGMELDDSEAMELLANAASGLTMAGLEILVPAALTRSLRAHATVD
                     SSWQRDGVGGLDLSAVVDLTWRATVDGEPVSEEELQRLADAQRPVVKLRGEWVVVDAA
                     VMSRIGRRDTLRASDALAAALGGFVSFDGQFVEVDVAGPIADLAERVRSAGALHDAVD
                     PFGLNATLRPYQRRRVAWIDEMARLRLGGVLADDMGLGKTIQVIATLLRRLDDPDVTA
                     IGPTLVVCPASVVANWQREITRFAPSLRCVAYVGLERSLPTLGADDVVVTTYGLARRD
                     IDELVEIDWQLVVADEAQHIKNPTSATARAMRRFTSGSRLAMTGTPVENRLADLWALL
                     DWTTPGLLGSLETFRRRIAIPIERDRDPLATERFARLIAPFVLRRRKDDPDIVPELPP
                     KTETVHPVVLSAEQAGLYRAVSQELMVEIEAASGLARRGLVFKLLTALKQICNHPAQY
                     LKQEGPLEHRSGKLEAFDELVDAICDAGDSVLVFTQYVVMGNLLSNRLRGRGIDARFL
                     DGSTPLDRRADLVDRFQEREFQVFIISLKAGGTGLNLTAATHVIHYDRWWNPAVENQA
                     SDRAWRIGQDRPVQIHQLICEGTLEERIAELLDEKRALADAVVGAGEAWLAELTNDDL
                     AELVELGVQA"
     gene            4158..5357
                     /locus_tag="PLP95_03485"
     CDS             4158..5357
                     /locus_tag="PLP95_03485"
                     /inference="COORDINATES: protein motif:HMM:NF016330.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SWIM zinc finger family protein"
                     /protein_id="HPB44893.1"
                     /translation="MTGFDDGSSSLGARWIDWLERRSGIDPTRLTAGRNQARRHPMRS
                     LVIDRGEARSEDTRVEFERFTSQRWRQVLEVLGTKSGHVAALLDGELSSAILDELADA
                     GIELFATADEIASSCRCDEWGDPCRHAAAVLYRLATEFDSDPLALVTLRGLRRDDLAA
                     LWNELRGIEPQPATVEEPDAAEIWAGVSLAEAESVELPPMPAGLGALLGAPELLAAPD
                     PWEPDLAAKDPIDPHSVDELALDATERAWAVLADGASAGLVGTVHGDLARRAANLQHT
                     PGMAMLASRTRQSVETLRSWAAAWSLGGDVAVEVLAEPRSRLIDQRRLAEGRDALVEM
                     GISKRTVALNYDSLGMPGGVKLVIGADGRWYRLRSEPGVRGRPETWLLDGPGSYEVED
                     LVGDDEA"
     gene            complement(5358..>6016)
                     /locus_tag="PLP95_03490"
     CDS             complement(5358..>6016)
                     /locus_tag="PLP95_03490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB44894.1"
                     /translation="ITRPRLAAATAPLLGVVAGRMIIEGYLRLNDITISTSRTSFLSI
                     GVQGFAEHHRLSPGWLIYSLWGPLWVAVAALALWCSRSPRATGAPRHAAVLLGIGAFA
                     SLAPMLITLDQTRVYSMVSAPVLAGAAILVGIRVIPSGGTVPSTRSTSPANGSAWISA
                     AALVVALLVPGMFNAGEAYFATDLPVGPFVDFLVHGTVPDDDLTGFLLAPFPFEIPGG
                     "
BASE COUNT          946 a         1872 c         2169 g         1029 t
ORIGIN      
        1 aagggggtcg cgtcctaccg aaccgacgcc gaggtgaacg acgagctgct ggtcgtcgac
       61 gacgacgagc cggtcgcatg gattctcggc caggccgacg aatcggatct cgaactgacc
      121 gacgaacaag tcgcggcggt gctgttgcag caggccgagt actaccggcg gatcggcgcg
      181 gtcggcccgc aggtcgcaac cccgtagtcg cggaggggcg aaccggggcg cgacggtttc
      241 ggcaccggac cgaaaggcgc gtaacatcgc cgctatgaag cgcctcggta agttcgaaca
      301 caaccgctac gtcggtgaca agcgcagcca ggttgtgtac gacatcgacg aactcgacga
      361 cgagtatcgg tcggtcatcg acgagttgat ggccgccgag acgtatctgt gtttcgggcc
      421 cgacaccctc gatgaggcgc gcaaccgcgg ctatcgcctg ttcggtccgc aacgacgcac
      481 cgccgagtcc gcggcgaact agctcgtcgc cgcatcatgg ccggcgaagc gacgatggac
      541 tcggtgctcg aggtcgacgg cttacgcctt tcggcgcaca ttgcccgccc gacacaggat
      601 cgcggcatcc ccgaatcgct gctgcccggg gtcgtcgtgt gtcatgggtt tcctgttcgg
      661 ggccgtgagg ctccggcttc cggcaagtcg tttccggagt tggccgatcg catcgcacac
      721 gagatgggct ggctcgcgat gacgatgaat tttcgcggtt gcggcaggtc cgagggcgac
      781 ttctcgctca gcggatgggt gcaagacatc gaggcggccg tcacgcatct gcaggatctc
      841 ggggcgagcg ccgtgtggtt ggcgggcttc ggcagcggtg gcgccctcac gttgtgcgcc
      901 ggcgctcgtc gacccgacgt tcgcggagtg gccgccgtct ccgcaccggc ggatttcagc
      961 gattgggcac gccatccccg tcgcctcgtc gagcactctc gaagcgttgg cgtgatcaag
     1021 actcccgggt atcccttcga cttcgaccga tgggcggggg agtttcgtgg gctcacggcg
     1081 gtgtcagcag ccgagcgttt ggcgccgcgg ccgcttctgg tgatgcacgg tgaggtcgat
     1141 gacctcgttc cgagtatcga cgcgcgtgcg attgccgacg cgcatgggaa tgctgaattg
     1201 cgaatcatcg ccggaggcgg tcacgagttg cgccacgacc ctcgggcgat cgcggtgctg
     1261 ttgggatggt tgaaccgcca gcacaacgcc gccgtggcgg acgcctgagc gacggaaccc
     1321 tccggattgc cccgtagact gttcggctat gcgttggagc ggtgacgggc ggattcgggg
     1381 gcgtcgatga caggttcgac gtttctgtcc cggccaggtc gggtacacgc agcattcatc
     1441 cccgccgaac cgccgaggtc ctcgcggctg gccctgtggc accgcgaccc cgaagtcgtg
     1501 gatgcgctcg aatccctggg ggcgcgcggc tcgatcgatg tggtgatccc cggtgacgtc
     1561 ggggttgaac gggttgaggt ggatgcggtc ttcctgccga tggcggccgc gatcgactgg
     1621 ttgatgggtt cgtcgaacga gatggcctcg acccatgcgc tcgcgcgggt ggtgcggtcg
     1681 gcgctcggct tggtcgcccg cggcctgctg caaccctcgg tgtccgacga cggtaccgac
     1741 cgctgggtgg tgtcgccgct cacctccgat gacctcatcg tccgatcgct gttggtgaca
     1801 cacctgcccc cgagtgcgca ctgcatcgcg accggtgtcg gggcggagcg gatgcaggcg
     1861 gcggctgagg tcgttcatgg cctgatcgct gcggtcgtgg actcgatgcc tcgaacgccg
     1921 ggagcggcgg ttgcggtcga ccaggtggcg tgggcgaccg gcgctgcggt cgacgtgtct
     1981 cggctccgtt cccgactggc gggcaccgag gtgcacgagc gtgtcacggt ggggttgcgg
     2041 gtgatcccac ctctggatga ggggctcgcg ttcacgtgtg aggttcaggt gcgctccacc
     2101 agcgacccgg ccgtgatttc gagtgctgcg cagttgtggg ccggcgaggt cgactgggtg
     2161 gatcgcagcg cagagttgga ggtgttgcgc accctgcgcc gcggggccga gcggtggaag
     2221 cccctcgagc gtctgttgga actgcccgag ccggtcggga tggaactcga cgattccgaa
     2281 gcgatggaac tcctcgccaa cgccgcctcc gggctgacga tggcgggcct tgagattctg
     2341 gtgcccgcgg cgttgacccg gtcgcttcgc gcccatgcga ccgtcgattc cagctggcag
     2401 cgcgatgggg tcggtggcct cgacctgtcg gcggtggtcg atctgacgtg gcgcgccacg
     2461 gtcgacgggg agccggtgtc ggaagaggag ctgcagcgcc tcgctgacgc gcagcggccc
     2521 gttgtgaaac tgcgcggcga gtgggtggtg gtcgacgcgg cggtcatgtc gcgaatcgga
     2581 cgtcgtgaca ccctccgtgc ctcggacgcg ctcgccgctg cgctcggcgg attcgtgagc
     2641 ttcgacggac agtttgtcga ggttgacgtc gccggtccga tcgccgacct cgccgaacgc
     2701 gtgcgttcgg cgggggcgtt gcacgacgcg gtcgatccgt tcgggctgaa cgcaacgctt
     2761 cgtccctacc agcggcgcag agtcgcgtgg atcgacgaga tggctcggct ccgcctcggt
     2821 ggggtcctcg cagatgacat gggtctcggc aagacgatcc aggtgatcgc aacgttgctg
     2881 cgccgtctcg acgatcccga tgtgaccgcg atcggaccga cgctggtggt ctgtccggca
     2941 tcggtcgtgg cgaactggca acgcgagatc acccgattcg cgcccagcct gcgatgcgtc
     3001 gcctacgtcg gcctcgagcg cagcctgccg acgttgggtg ccgatgacgt ggtggtcacc
     3061 acctatgggc tcgcccgtcg agacattgac gagttggtcg agatcgactg gcaactcgtc
     3121 gtcgccgacg aggctcagca catcaagaac ccgacctcgg cgacggctcg cgccatgcgc
     3181 cgtttcacct ccggttcgcg cctggcgatg accggtactc ccgtcgagaa ccgactggcc
     3241 gacctgtggg cgctgctcga ctggacgaca ccggggttgc tgggttcgct ggagacattc
     3301 cgacggcgca tcgccattcc catcgaacgc gaccgcgacc cgctcgccac cgaacggttc
     3361 gcccggttga tcgccccgtt cgtgctgcga cgtcgcaaag acgaccccga catcgtcccc
     3421 gagctgccgc cgaaaaccga gaccgtgcat cccgtcgtgc tgagcgccga acaggccgga
     3481 ctgtaccgcg cggtgtcgca ggagctcatg gtcgagatcg aggcagcgag tggactggcg
     3541 cggcgaggcc tggtgttcaa actgctcacc gccctcaagc agatctgcaa tcacccggcg
     3601 caatacctca aacaggaggg gccgctcgaa catcgttccg ggaagttgga ggcattcgac
     3661 gaactcgtcg acgcgatctg cgacgccggc gattcggtgc tggtgttcac ccagtacgtg
     3721 gtgatgggca acttgttgtc gaaccggctg cggggccgtg gaatcgatgc ccgcttcctc
     3781 gacggctcga ccccgttaga tcgtcgcgcc gatctcgtgg accggttcca ggaacgcgag
     3841 ttccaggtgt tcatcatctc gctcaaggct ggcggtaccg gcttgaacct gaccgccgcg
     3901 acccacgtca tccactacga ccgctggtgg aatccggcgg tcgagaatca ggcgtcggac
     3961 cgggcgtggc gcatcggcca ggaccgcccg gtacagattc atcagctcat ctgcgaggga
     4021 acgctcgagg agcgtatcgc cgagttgctc gacgagaagc gggcgttggc cgacgcggtc
     4081 gtcggagccg gcgaggcgtg gctcgccgag ctgaccaatg acgacctggc agaactcgtg
     4141 gagttggggg tgcaggcgtg acgggtttcg acgacgggtc gtcgtcgctc ggagcgcggt
     4201 ggatcgactg gttggagcgt cgcagcggca tcgacccgac caggctcacc gccggcagaa
     4261 atcaagcgag acgtcatccg atgcgttcgt tggtgatcga ccgcggggag gctcgtagcg
     4321 aggacacccg ggtcgagttc gagcggttca cctcccagcg ctggcgtcag gtccttgagg
     4381 tgcttggcac aaagtcggga catgtcgctg cactcttgga cggcgaactg tcgagcgcga
     4441 tcctcgacga actggcggac gccgggatcg aactgttcgc gaccgccgac gagatcgcct
     4501 cgagttgtcg ctgcgacgaa tggggtgacc cgtgccgcca cgccgcggcg gtgctgtatc
     4561 gcctcgccac cgaattcgac agcgacccgt tggcgctggt gacacttcgg gggctgcgtc
     4621 gcgatgacct tgctgcactc tggaatgagc ttcgcggaat cgagccgcag ccggcgaccg
     4681 tcgaggagcc agatgccgcc gagatctggg ctggcgtgtc ccttgcggaa gccgaatctg
     4741 tcgagctccc gccgatgcca gcaggcttgg gggcgctgtt gggggctccc gagcttctcg
     4801 cggcaccgga tccgtgggaa cccgacttgg cggcgaagga cccgatcgac ccccacagcg
     4861 tcgacgagct ggccctcgat gccaccgaac gcgcctgggc ggtgcttgcc gatggcgcct
     4921 cggcgggtct cgtcgggacg gtgcacggcg acctggcgag gcgtgcggcc aatctccagc
     4981 acacgccagg gatggcgatg ctggcgtcga gaaccaggca gagtgttgaa acgctgcggt
     5041 cctgggcggc ggcgtggagc ctcggtggcg atgtcgccgt cgaggtgctt gcggagccgc
     5101 gatcgcggct catcgatcag cggcggctgg ccgaggggcg cgatgcgctc gtcgagatgg
     5161 ggatctcgaa gcggacggtc gcgctgaact acgactcgct cggaatgccg ggtggggtga
     5221 agttggtgat cggtgccgac gggcggtggt accggttgcg ctccgagccc ggggtccgcg
     5281 gtcgccccga gacctggctg ctcgacggtc cgggatcgta cgaggtcgag gacctggtgg
     5341 gcgacgacga ggcgtgatca gccgccggga atctcgaagg ggaagggtgc cagcagaaac
     5401 ccggtgagat cgtcgtcggg gacggtgccg tgcacgagga agtcgacgaa gggacctacc
     5461 ggaaggtccg ttgcgaagta ggcctcgccg gcattgaaca tcccgggaac gaggagggcc
     5521 acgacgagtg ccgccgcgct gatccaggcc gatccgttgg ccggcgaggt ggaccgcgtc
     5581 gagggaaccg tcccaccgga tggaatcacc cggatcccca ccaggattgc tgcacctgcg
     5641 agcacgggtg cgctcaccat cgaatacacg cgggtctgat cgagggtgat cagcatcggt
     5701 gccagcgacg cgaaagctcc gatgcccagg agcactgcag cgtgacgcgg ggctccggtc
     5761 gctcgtggtg aacggctaca ccacagtgcg agtgcggcaa cggcgaccca caacggcccc
     5821 cacagcgagt agatcagcca gccgggtgac aggcgatggt gctcggcgaa tccttgaacg
     5881 ccgatggaga gaaacgacgt ccttgaggtc gaaatggtga tgtcgttgag gcgaaggtag
     5941 ccctcgatga tcatgcggcc ggcgacgact ccgagcagcg gtgcggtggc cgcggccagg
     6001 cgcggtctgg taatcc
//
LOCUS       DAOOMA010000079         6014 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_15709_length_6014_cov_3.621749, whole genome shotgun sequence.
ACCESSION   DAOOMA010000079 DAOOMA010000000
VERSION     DAOOMA010000079.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 6014)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6014)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6014
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15709_length_6014_cov_3.621749"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..534)
                     /locus_tag="PLP95_03495"
     CDS             complement(<1..534)
                     /locus_tag="PLP95_03495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HPB44895.1"
                     /translation="MDQAAVDSLIVLLDLEPIEVNIFRGQSPDEERQRAFGGQVAGQA
                     LVAATRTVEAGFGVHSLHGYFLRSGDVGVPILYEVARIRDARSFCTRRVQAIQHGRPI
                     FGLTASFQRAEEGYEHQVPMPLGLPEPESLPDFRDRMAPYRHLLGSWYDRPRAIDTRY
                     CNWQPPDDRSPGTLIDDV"
     gene            619..1131
                     /locus_tag="PLP95_03500"
     CDS             619..1131
                     /locus_tag="PLP95_03500"
                     /inference="COORDINATES: protein motif:HMM:NF016060.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HPB44896.1"
                     /translation="MKLTPSGLIEHARSDEGRKQLRYAAVSVVFVPLGQVLVQVLRWV
                     FEVREYWAVLISACILTIPNYLANKFMVWRDKSKDNRTTEVIVFWSAAVLGTGFAMGF
                     VWLAGQWFPEDRGKAVHGFAIFIAQLVGYGIVWVARYVFLDRWLFKATHHGEEPPREF
                     LEDLHGELPL"
     gene            1155..1823
                     /locus_tag="PLP95_03505"
     CDS             1155..1823
                     /locus_tag="PLP95_03505"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012224985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HPB44897.1"
                     /translation="MDETLRSAAQAARGFMPIDEGDALYEAGLEATRRVPGLPLLEIG
                     SYCGKSGVYLGAAARAGEVVLFAVDHHRGSEENQPGWEWHEPDLVDPAVNKMDTLPIF
                     RRTIHDAGLERHVVAVVADSPTLSRFWTTPLALLFIDGGHGEEPAHRDYERWSAHVAP
                     GGILAIHDVFPNPSDGGRPPYEIYLRALESGNFTELSATGSLRVLARADNARVHRPEP
                     KRFD"
     gene            complement(1846..2256)
                     /locus_tag="PLP95_03510"
     CDS             complement(1846..2256)
                     /locus_tag="PLP95_03510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB44898.1"
                     /translation="MNAAGFAHRVRAFGAAAFVALAIGLFSAAPHAGAQDDGSEIATT
                     TTLAVSSATEPELRPGEAPSIIPVPHSSGDYEVQPGEPGSGEQYLVFAVIIGGLATIV
                     IAVTLESRRKREEFGTKMPDPTTIYEQDDPTAER"
     gene            complement(2253..3329)
                     /locus_tag="PLP95_03515"
     CDS             complement(2253..3329)
                     /locus_tag="PLP95_03515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB44899.1"
                     /translation="MWLHDVDGIVTADQLSETVDYIASLQLPNGMVQWFEGGHADPWN
                     HTEALMALALGGRIDEARLGFSWLRDLQRPDGAWHQYYLADRVEQDKLDANVIAYVAA
                     GVWHHWLLTGDRAWLESMWPMVDRAIGFVLELQTPRGEIIWARHADGTPWTFALLTGS
                     SSMLHSIRCAVAIADTLDQARPEWELSAARLGNTIRNVPEAFAPKHRWAMDWYYPVLC
                     GAIVGEAAQQRIDERYDAFVEESRGVRCVSDRPWITAAETCELVMALLAIDDRERGEA
                     LFGWAQRHRDDEGRYWTGIVYPDEVHFPGGEQSSYTAAAVVLGADALAAKSPASHLFT
                     DHGAFPALIDIGADDAVTEHSGDA"
     gene            complement(3316..4074)
                     /locus_tag="PLP95_03520"
     CDS             complement(3316..4074)
                     /locus_tag="PLP95_03520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HPB44900.1"
                     /translation="MLTIDYDRLDLQPGMSLLDMGCGAGRHSFEAFRRGAQVVALDYS
                     FSDLESVRDLFWAMHETDEVPRTHHAQAVRGDGYQLPFADDTFDRIICSEVLEHLNDD
                     AAAMAELFRVLKPGGRIAVSVPTWLTEKICWTLSAEYHAPLAEGGHLRIYTERLLRER
                     LETTGFTLGESHREHALHSPYWALRCIVSPTNSSHPLVKAYHDFLVWDMMKAPAITRA
                     ADRLLNPILGKAVVVYGEKPNTPTHRRSDDRVAA"
     gene            complement(4084..5361)
                     /locus_tag="PLP95_03525"
     CDS             complement(4084..5361)
                     /locus_tag="PLP95_03525"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HPB44901.1"
                     /translation="MQNNSKRRPGDDDPLRIAYLTYRGKPHVGGQGVYTRHLTKALVD
                     LGHSVEVLSGQPYPIVDPRVPLVQLPSLDIYNDHFPMRMPGLWELKNWTDFAEVTAFS
                     TGTFPEPLAFSLRAWDHLKHRVNEFDLVQDNQCLGYGLLAIESAGLPVLGTIHHPITV
                     DRRIEMEHAETTYQRLSKARWYAFTKMQTRVARRLKRIITVSQNSFDDICRTHDVSPD
                     KLFVVPVGVDPDLFKPLDDIERKPFQIISTASADVAMKGQRFLLEALAKLRAEFPELK
                     LVIIGKLKEGSVAKRTIEELGLDGVVEFVSGVPDERIVEMYNESTCAVVPSLYEGFSL
                     PAIEAMATGCPLVATTGGALPEVTGKDGDTCYSCEPANSDALAAAIRRAIENPDDSKR
                     IGIAGRERVITRWSWRHTAVRTVDHYRARLALS"
     gene            complement(5565..>6014)
                     /locus_tag="PLP95_03530"
     CDS             complement(5565..>6014)
                     /locus_tag="PLP95_03530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB44902.1"
                     /translation="AAPTTAAPTTAAPTTAAPTTTAAPAGDDVVTVAIRGGLNYNASS
                     SLTSGNLKVALTNRSASAISGSGTYPGVNGGTARVTVNASNFLWWSFGTISVNDPGAG
                     IRNLSTPLVFASPVSGSLSSARATGSWLTWNDGLVNYTVAITVADNG"
BASE COUNT         1028 a         1850 c         2033 g         1103 t
ORIGIN      
        1 gacatcgtcg atcaacgtcc ccggcgagcg gtcatcaggc ggctgccagt tgcagtagcg
       61 ggtatcgatg gcccgcggcc ggtcgtacca ggagcccaac aggtggcggt agggggccat
      121 gcggtcgcga aagtccggta gcgactcggg ttcgggcaga cccaacggca ttggcacctg
      181 atgctcatag ccctcttcag cgcgttgaaa cgacgcggtc agcccgaaga tcggacgtcc
      241 gtgttgaatc gcctgaaccc gacgggtgca gaagcttcgg gcgtcgcgaa ttcgcgcgac
      301 ctcatacagg atcgggaccc cgacatcgcc gcttcgcaga aagtatccgt gcagcgaatg
      361 gacgccgaac ccagcctcca cggtccgggt cgccgcaacg agcgcctgcc cggcgacctg
      421 gccgccgaag gcgcgctggc gctcctcgtc ggggctttga cctcgaaaga tgttcacctc
      481 gatcggttcg aggtccaaga gaacgatcaa cgaatccacg gcggcctgat ccatgccaac
      541 catcttgccc gacgccgacg gatgaacgga tcgtcgttga ttcggactgc cgtcgtcagg
      601 gcgatacgat ctcacgtcgt gaagctgact ccctcgggac tgatcgagca cgccaggagc
      661 gacgagggcc gaaagcagct tcgttacgcc gcggtctcgg tggtgttcgt gccgctcgga
      721 caggtgctgg ttcaggtgct gcgctgggtg ttcgaggttc gcgagtactg ggcggtgctg
      781 atttcggcat gcatcctcac catcccgaac tacctggcca acaagttcat ggtgtggcgc
      841 gataagtcga aggacaatcg aaccaccgag gtcatcgtgt tctggagcgc agcggtgctc
      901 gggaccggat ttgcgatggg cttcgtctgg ttggccgggc aatggttccc cgaagaccgt
      961 ggtaaggcgg tgcacggttt cgccatcttc atcgctcagc tcgtcggcta cggcatcgtt
     1021 tgggtcgccc ggtacgtgtt tctcgaccgc tggctgttca aagcgaccca ccacggcgaa
     1081 gaaccgccca gggagttcct cgaagacctt cacggcgagc ttccgctgtg acgggcaccg
     1141 gcaacgaggc ccccatggac gagaccttgc gcagtgccgc tcaggcggcg cgtggcttca
     1201 tgccgatcga cgagggtgat gccctgtatg aggcgggcct cgaggcgaca cgtcgggtgc
     1261 cgggcctccc gctgttggag atcggaagct actgcggtaa gtccggggtc tacctcgggg
     1321 ccgcggcgcg agccggtgag gtcgtgttgt tcgcagtcga ccatcaccgc ggatccgagg
     1381 agaaccaacc gggttgggaa tggcacgaac ccgaccttgt cgatcccgcg gtgaacaaga
     1441 tggacacgtt gccgatcttt cggcgcacga tccacgatgc cggtctcgaa cgccatgtcg
     1501 tggcagtcgt tgccgactct ccgaccctca gccgcttctg gacgacgccg ctggccctgc
     1561 tgttcatcga cggtgggcat ggcgaggagc cggcgcatcg tgattacgaa cgctggagcg
     1621 ctcacgttgc ccccggcgga attctcgcca tccacgacgt gtttccaaac ccttccgacg
     1681 gcggccgtcc accctatgag atctacctcc gggcgttgga gtcggggaac ttcaccgaac
     1741 tctcggccac cggctcgttg agggtcttgg cgcgagccga caacgctcgg gttcaccgac
     1801 cagagccgaa gcggttcgac tgacggccga cgcctccagg cggcgctaac gctcggcggt
     1861 cggatcgtcc tgttcgtaga tcgtcgtggg gtcgggcatc ttggtgccga actcctcgcg
     1921 tttgcggcgt gactcgaggg tcacggcgat gacgatcgtc gcgagtccgc cgatgatcac
     1981 cgcgaagacc aggtactgct ccccgctgcc cggctcgccg ggttggactt cgtagtctcc
     2041 tgaggagtgt ggaaccggga tgatcgaggg ggcctcaccc gggcgcaact ccggctctgt
     2101 cgcgctcgac accgccagag tcgtcgtggt cgcgatttcg ctgccgtcgt cttgtgcccc
     2161 agcgtgcggc gctgcgctga acagcccgat ggcaagtgcc acgaacgcgg ccgccccaaa
     2221 ggctcgcacg cggtgcgcga acccagccgc gttcacgcgt ctcctgaatg ctcggtgacg
     2281 gcgtcgtcgg ccccgatatc gatcagtgca gggaacgcgc cgtggtcggt gaacaggtgc
     2341 gatgcagggc tcttggccgc aagcgcgtcg gcccccagca ccactgcagc agcggtgtac
     2401 gacgactgct cgccacccgg aaagtgcacc tcatcggggt acacgatgcc ggtccagtag
     2461 cggccctcgt catcgcgatg ccgctgggcc cagccgaaca gggcctcgcc gcgttcgcga
     2521 tcgtcgatcg ccaacaaggc catgaccaac tcgcaggtct cggccgcggt gatccacggt
     2581 cgatccgaca cgcaccgcac gccccgggat tcctcgacga acgcgtcgta gcgctcatcg
     2641 atgcgctgtt gggcggcctc gccgacgatc gccccgcaca gcaccgggta gtaccagtcc
     2701 atcgcccaac ggtgcttggg agcgaacgcc tcgggcacgt tgcggatcgt gttgccgagc
     2761 cgcgcggcgg acaactccca ttccggtcgg gcttgatcca gggtgtcggc gatagcgacc
     2821 gcgcagcgga tgctgtgcaa catcgatgac gaaccggtga ggagtgcgaa ggtccacgga
     2881 gtcccgtcgg cgtggcgggc ccagatgatc tcgcctcgag gtgtctggag ttccagcacg
     2941 aacccgatcg cccggtcgac catcggccac atcgattcga gccaggcgcg atccccggtg
     3001 agaagccagt ggtgccatac cccagcggcg acgtacgcga tcacgttggc gtcgagcttg
     3061 tcctgttcga cgcggtcggc gaggtagtac tggtgccagg cgccatcggg gcgttgaagg
     3121 tcgcgaagcc aactgaatcc gagccgggct tcgtcgatcc gtccgccgag ggccagcgcc
     3181 atcaacgcct ctgtgtggtt ccacgggtcg gcgtgaccgc cttcgaacca ctggaccatt
     3241 ccgttcggaa gttgcaggga tgcgatgtag tcgacggttt cgctcagttg gtcggccgtg
     3301 acgatgccgt cgacatcatg cagccacacg gtcgtcgctc ctacggtgag ttggggtgtt
     3361 cggcttttct ccgtagacga cgacggcctt gcccaggatg gggttgagga ggcggtcggc
     3421 agcccgtgtg atggcaggtg ccttcatcat gtcccacacc aggaagtcgt ggtacgcctt
     3481 gacgagcgga tgtgaggaat tggttgggct gacgatgcag cgcagcgccc aataaggaga
     3541 gtgcagcgcg tgctctcgat gggactcccc gagggtgaac cccgtggttt ccaggcgctc
     3601 acggagcagt cgttcggtgt agatgcgcag gtgaccaccc tcggcgaggg gggcgtgata
     3661 ctcggctgag agggtccagc agatcttctc ggtcaaccac gtgggaacgg acacggcgat
     3721 acgcccaccc ggcttcagca cccggaaaag ttcggccatc gccgcggcgt cgtcgttcag
     3781 gtgttcaagg acttccgagc agatgatgcg gtcgaaggtg tcatccgcga acggcaactg
     3841 gtagccgtcg ccacgaacgg cctgagcatg gtgggtcctg gggacctcgt cggtctcgtg
     3901 catcgcccag aacaaatcgc ggaccgattc gaggtcgctg aaggaatagt cgagcgcgac
     3961 cacctgggcc ccgcgtcgaa acgcctcgaa actgtggcgg ccggccccgc accccatgtc
     4021 gagcagtgac atgccgggct gcaggtcgag gcggtcgtag tcgatggtca acacgggcag
     4081 gtcctagctc aacgcgagtc gcgcacgata atggtcgacg gtgcgcaccg cggtgtgtcg
     4141 ccaactccag cgggtgatga cccgttcgcg tccggcgatg ccgattcgct tcgagtcgtc
     4201 cggattctcg atcgctcggc ggatcgccgc tgcgagcgcg tcgctgttgg ccggttcgca
     4261 cgagtagcag gtgtcgccgt ccttgccggt gacctcgggc agcgccccac cggtggtcgc
     4321 gacgagaggg catccggtgg ccatcgcttc gatcgccggc aaggagaatc cctcatacaa
     4381 cgagggcacc acggcgcagg tcgattcgtt gtacatctcg acgattcgtt cgtcggggac
     4441 gccggatacg aactcgacga caccgtcgag accgagttcc tcgatcgtcc gcttcgccac
     4501 agacccctct ttgagtttgc cgatgatgac gagcttgagc tcggggaact cggctcggag
     4561 tttcgcgagg gcctcgagaa ggaaccgctg ccccttcatg gcgacgtcgg ccgaggccgt
     4621 cgagatgatc tgaaagggct tgcgctcgat gtcgtcgagc ggcttgaaca gatccgggtc
     4681 gaccccgacc ggcacgacga acaacttgtc gggactcacg tcgtgggtcc ggcagatgtc
     4741 gtcgaaactg ttctgggaga cggtgatgat tcgcttgagg cgtctcgcca ctcgggtctg
     4801 catcttggtg aacgcgtacc accgtgcctt ggaaaggcgt tggtaggtcg tttccgcgtg
     4861 ttccatctcg atgcggcgat cgacggtgat gggatggtgg atcgttccga ggactggaag
     4921 cccagcggac tcgattgcca acaggccgta gccgaggcac tgattgtcct ggacgagatc
     4981 gaactcgttc acgcggtgct tgaggtggtc ccaggctcgc agcgaaaacg cgaggggctc
     5041 gggaaaggtt ccggtgctga acgcggtcac ctcggcgaag tcggtccagt tcttgagttc
     5101 ccagagccca ggcatccgca tcgggaagtg gtcgttgtag atgtcgagac tgggcaactg
     5161 aacgagcggg acccgcgggt cgacaatcgg gtagggctgg cccgacaaca cttcgacgga
     5221 gtgcccgagg tcgacgagtg ccttggtcag gtggcgtgtg tagacgccct gcccacccac
     5281 atgcggcttc ccccgatacg tcaggtacgc gatccggagg ggatcgtcgt cacctggccg
     5341 tcgcttcgag ttgttctgca taacaaacca gcttctctgc ccaggcaggc gccggtcaaa
     5401 ccctgcgagc ggccacctcg gcgatcgatg tgttgtggaa ttctgggcgc gatgttcgag
     5461 tgatcgaatt cgtggccggg gggtggggtt gggacgtgaa acgggcgggg ttcgtatgaa
     5521 ccccgcccgc cggacgattc attcccaggg gtgttcagtt gctctcagcc gttgtccgca
     5581 acggtgatgg cgacggtgta gttgaccagg ccgtcgttcc aggtcaacca gctgccggtt
     5641 gccctggcgg aactcaacga ccctgagacc ggggaggcga agaccagggg cgtggagagg
     5701 ttgcgaattc ctgctccggg gtcgttcacc gagatggtgc cgaagctcca ccacaagaaa
     5761 ttggaggcgt tcacggtgac tcgcgccgtg ccaccgttga cgccggggta ggtgccactg
     5821 ccggagatgg cggaagccga ccgattcgtg agcgcgacct tcaagttgcc gctcgtcagg
     5881 ctgctggagg cgttgtagtt cagcccacca cggatcgcta ccgtgacgac atcatcgccg
     5941 gcgggggcag cggtcgtggt cggtgcagcg gtagttggtg cagcggtagt tggtgcagcg
     6001 gtggtcggtg cagc
//
LOCUS       DAOOMA010000080         5997 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_15781_length_5997_cov_3.957758, whole genome shotgun sequence.
ACCESSION   DAOOMA010000080 DAOOMA010000000
VERSION     DAOOMA010000080.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 5997)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5997)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5997
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15781_length_5997_cov_3.957758"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            197..273
                     /locus_tag="PLP95_03535"
     tRNA            197..273
                     /locus_tag="PLP95_03535"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:231..233,aa:Val,seq:tac)
     gene            369..2591
                     /locus_tag="PLP95_03540"
     CDS             369..2591
                     /locus_tag="PLP95_03540"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HD-GYP domain-containing protein"
                     /protein_id="HPB44903.1"
                     /translation="MDFERNPVDRTAPARPDSELSASDVVDGDVVDREALRRAMARAA
                     TERANHPAPHTVVAHRSSMVDGSVEVLEAPEAPEAQVAPEHAAAVGSVKATKFVEQRS
                     ARAGDDQSPTRSWESRPIASRLVRLGVTLVPIAVGALFAVAASRVLPQATSVVMAVLR
                     ALLVVALTTGVVRLVDKASRRFLPLAALLQLSLVFPDQAPDRMRAALKAGSSRRLARN
                     VEEARTNGLASDPGDAARQVVALISAIGDHDRRTRGHSERVRLFADLIGEELHLSKEE
                     RQKLQWGALLHDLGKLMVPDEILNKKGKPTDEEWSILQSHPAEGMRLVEPLREFLGEW
                     VYAIGGHHEKWNGTGYPEGLRGDGIPRAAAIVAVADSFEVMTAVRSYKRAMPLSEARA
                     ELTRCSGTHFSPEVVRAFLKVSLGDLRLVSGPLASLAQVPFVQSIMSAPAQVGGLASP
                     VLGAAAAAPAAAAATAAAVVAAVAGAVAVVGSGPATADEPPAPVESAIVTTVPELSET
                     VTYGSSPNAGKAPVGPEGAGTEAAPATSTTTTTTTTTTAPATTAPPTSFQVSGTVKPD
                     SPPQTPQSPTAPASGPSPSGTPDGAGGSASASNARKTPASTAAPTTAAPPSAPLPPPN
                     GQGTAATTLSLVVRQEGSSTSTSLEGANLQAGTRYVVTAMSNQMPNIKRVTYVYGDGL
                     LVQATTTAPFEFTFTATSLLAGNSTQSVTALVEVYSGSPFSKKVNFTTTSGCLLGLLC
                     "
     gene            complement(2610..3920)
                     /locus_tag="PLP95_03545"
     CDS             complement(2610..3920)
                     /locus_tag="PLP95_03545"
                     /inference="COORDINATES: protein motif:HMM:TIGR00797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0009410 - response to xenobiotic stimulus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HPB44904.1"
                     /translation="MPTTASASIDRQIGAIAIPAALALATEPLAELCDTAIVGTLGTT
                     ALGGVAIAVRVVSFASTVFIFLMFATTTAVARHLGAGDARRAAHEAVTAMWIALAIGA
                     TATVTLLIGGPTFISWLGGSGEVARDAGRYLEIVALSAPGSTLTMAGIGARRGHLDTR
                     IPLLISAVAAATNLVADLILVIGLGFGIATAAWTTTATALASGITYVVLVVRDARRAG
                     APLVADRRSLRTQLVVGRDLLVRTVALRTVLTVATALAARQGEVTLAAYAIAFQIWIF
                     CSYALDGLEAAGQSLVSHRLGSATFHELGTVTARLFTWGVRVGVALAVGIVALSWWLP
                     RVFSSDQAVIDAATTALWWVAAFAPVSAVAFVGDGMLVGASRQRFLAATMLIASCAFG
                     FVAWAAAPWGLAGIWAAISAMMVVRTAGAALGISRLRTMAASAA"
     gene            4038..4673
                     /locus_tag="PLP95_03550"
     CDS             4038..4673
                     /locus_tag="PLP95_03550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB44905.1"
                     /translation="MRNDGTRERGAASGAWAAAAVVLALGALLFGFTAHVRLNEMEAE
                     VARLSWATTTVPVGEPRVATDEGLGLGVGADSEQAGSASAGTAQAGSGDSAPSDDDRL
                     AVVRAFTTVYDASQPLSERLLSVDDPSGVDLALQAIEAGPNGELVKQVVVSVNDVKFP
                     TANSATVIYTIVVPGADPVPGRNGGAKLIDGAWKVTRATVCGDLAAVGGSC"
     gene            complement(4720..5277)
                     /locus_tag="PLP95_03555"
     CDS             complement(4720..5277)
                     /locus_tag="PLP95_03555"
                     /inference="COORDINATES: protein motif:HMM:NF013621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB44906.1"
                     /translation="MSGHRGGVGVFPGTFNPPTVAHLAVAEEARTTLELERVDLVVSR
                     IPIGKEHLSTPSLDQRVEVLERLAGRVGWMRIVVSEQRLIVDIAAGYDVVIMGADKWG
                     QVNDPVYYGGDSARRDDAVARLPRLALFDRADVAFNAGVRHITEPDAVTLRLEARYLD
                     VSSSAARAGASHLMVPEALESGLWS"
     gene            complement(5274..>5997)
                     /locus_tag="PLP95_03560"
     CDS             complement(5274..>5997)
                     /locus_tag="PLP95_03560"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HPB44907.1"
                     /translation="GSGASLRRSQQGAAEDEPVGAPAARPTGSTATSTGSAATHHALE
                     AIANPARLRRAIWAFFCLQPFSQMLVFAVNGDWFAGHLAMSNTQLSFATAALGVAELV
                     GVAWVIAYADRLGPVRVGGVAIGLAAIPSLILIAMSNSAVGNSAVFGVAMLLLMDLAM
                     EASFVATLPIVTELDVDNRGRSVAQAFVVMLVSRAVASAAGGAIYTRLGFNATLLTSA
                     TLCVAGCAALTWARRGPRLEAT"
BASE COUNT          997 a         1986 c         2120 g          894 t
ORIGIN      
        1 cgcccatccc gcatcggcgg agatcgtcga ggtcgcgtgg ctggatgcaa gctcggatgc
       61 aaccatcacc ccagagtcgc gagaggccct tgaggcgctc gaacgatctg aaggtgtcgc
      121 caccgggcga acgcctgggc agtgacgtcg tcgaatcggc cgtggggttg ccgggcggta
      181 tgctggcgaa ccgttcgggc gtatagctca gttggctaga gctcctggtt tacatccagg
      241 cggtcggggg ttcgagtccc tctgcgccca ccacccgcgt gcccactatc tcataggtct
      301 tttgggcacc ggagactgtg acgatcgccc ggtcaaactg ggcctcaggg ttgccgataa
      361 ccatcttgat ggatttcgag cgtaaccctg tggaccggac cgctcctgca cggccggaca
      421 gtgagctgtc agcgagcgac gtcgtcgacg gcgacgtggt cgatcgcgag gcgcttcgcc
      481 gagcgatggc tcgcgccgcg accgagcgcg cgaaccatcc ggcgccacac accgtggtcg
      541 ctcatcgttc gtcgatggtc gacgggtccg ttgaggtgct cgaggcaccc gaggcgcccg
      601 aggcgcaggt cgcgccggaa catgcagcgg cggtcggctc ggtgaaggcg acgaagttcg
      661 tcgaacagcg ctccgctcgt gcgggcgacg atcagagccc gacgcggtcc tgggagtcgc
      721 gtccgattgc gtcccgcctg gtgcgcctcg gtgtgacctt ggtaccgatc gccgtcggtg
      781 cgctgttcgc ggtggcggcg tcgagggtgc tacctcaagc gacgtcggtc gtgatggcgg
      841 tccttcgcgc gctcctggtg gtcgcactca ccaccggggt cgtgcgcctc gtcgacaagg
      901 cctcgcgccg gttcctgccg ttggcggcgc tgttgcagct gtcgttggtg ttcccggatc
      961 aagcaccgga ccgaatgcga gccgcactca aggccggaag ttcgcgtcgg ctcgcccgca
     1021 acgtcgagga agctcgaacg aacgggttgg catcggatcc gggtgatgcc gctcgccagg
     1081 tcgtcgcgct gatctcggcg atcggcgacc acgatcggcg cacgcgtggc cattccgaac
     1141 gcgtccggct ctttgcggac ctgatcggtg aggaactcca cctgtccaag gaggagcgac
     1201 agaagctcca atggggtgcg ctgttgcacg atctcggcaa gttgatggtt cccgacgaga
     1261 tcctcaacaa gaagggcaag cccaccgacg aggagtggtc catccttcag agccatccgg
     1321 ccgagggcat gaggctggtg gagccactgc gggagttcct cggagagtgg gtctacgcga
     1381 tcggcggcca ccatgagaag tggaacggca ccggctatcc cgaaggcctt cgcggtgatg
     1441 ggattccgag ggcggcggcg atcgtcgcgg tcgccgattc gttcgaggtg atgaccgcgg
     1501 tgcgctccta caagcgtgcg atgccgttga gcgaggcgcg agcggaactg acccgttgtt
     1561 cgggaacaca tttctcaccg gaggtggtgc gggcgttcct gaaggtgtca ttgggggatc
     1621 tgcgtctggt gtcggggccg ctcgcatcgc tggcgcaggt tccgttcgtc cagtcgatca
     1681 tgagcgctcc tgcacaagtc ggtggcttgg cgtcgccggt cctcggtgct gcggcagcgg
     1741 ctccggcggc ggccgcagcg accgcggcgg cggttgtcgc tgcggtggcg ggcgcagtag
     1801 cggtggtcgg gtccgggccc gccaccgccg atgaaccccc agcgccggtc gagtcagcca
     1861 tcgtcaccac ggtgcccgag ctcagcgaaa ccgtgaccta tggcagctcg ccgaacgccg
     1921 gcaaggcgcc ggtcgggccg gaaggcgccg gaaccgaggc tgctccagcg acctcgacga
     1981 ccacgacgac gaccacgacg accactgcgc cggcgacgac ggcgccgccg accagcttcc
     2041 aggtcagtgg cacggtcaag cccgactcgc caccgcaaac gccgcagtct ccaactgcgc
     2101 ctgcctcagg cccatctcca tcaggcacgc cggacggcgc cgggggttcg gcctcggcat
     2161 cgaatgcgcg caagacacca gcgtcgaccg cggcgccgac gaccgccgcg cccccttcgg
     2221 ctccgctgcc accgccgaat ggccaaggca ccgctgcgac aaccctttcc ctggtcgtgc
     2281 gtcaggaggg gtcgtcgacc tcgacatcgc tcgagggggc caacctccag gccggaaccc
     2341 gatatgtggt gacggcgatg tcgaatcaga tgccgaacat caaacgtgtc acctacgtct
     2401 acggcgacgg gctgttggtg caggcgacca cgacggcacc gttcgagttc accttcaccg
     2461 cgacatcgtt gctcgcgggc aacagtaccc aatcggtcac cgcgttggtc gaggtgtaca
     2521 gcgggtcacc gttctcgaag aaggtcaact tcacgaccac ctccggctgc cttctcggat
     2581 tgttgtgctg aacgccgacc cggtgaccct caggccgccg aggccgccat cgttcgcagc
     2641 cggctgatgc ccaatgcggc ccctgcggtg cgcacgacca tcatggccga gatcgcggcc
     2701 cagatccctg ccagccccca cggtgcagca gcccacgcga cgaacccgaa cgcacacgag
     2761 gcgatcagca tcgtggcggc caagaagcgt tgacgcgatg cgccgaccag catcccgtct
     2821 ccaacgaacg cgacggcgct gaccggggcg aacgctgcga cccaccacag ggccgtcgtc
     2881 gccgcgtcga tcaccgcctg atcggagctg aacacccggg gaagccacca cgacagtgcg
     2941 acgatgccaa ccgcgagcgc gaccccgacc cggactcccc acgtgaacag ccgtgcggtg
     3001 acggtgccga gttcgtggaa cgttgcggac cccaaccggt gcgacaccag cgattgtccg
     3061 gcggcctcga ggccgtcgag cgcgtacgag cagaagatcc agatctgaaa ggcgatcgcg
     3121 taggccgcca gggtcacctc accttgtcgc gccgcgagcg ccgtcgcgac ggtcaacacg
     3181 gtgcgtagcg caacggtgcg caccaacagg tctctgccga cgacgagttg cgtgcgcagg
     3241 ctccgccgat cggcgacgag cggcgccccg gcgcgccggg catcgcggac cacgagcacc
     3301 acgtaggtga tccccgatgc gagtgcggtc gcggtggtcg tccacgccgc ggtagcgatc
     3361 ccgaacccga ggccgatcac caggatcagg tcggcgacga ggttggtggc cgcggcgacc
     3421 gcactgatga gcagagggat ccgcgtatcg aggtgacccc ggcgcgctcc gatgccggcc
     3481 atcgtgaggg tggacccggg tgccgagagc gccacgatct cgaggtagcg accagcgtct
     3541 cgcgccacct cgccggatcc tccgagccat gagatgaacg tcgggccgcc gatcaggagt
     3601 gtgacggttg ccgtcgcgcc gatggcaagg gcgatccaca tcgcggtgac ggcctcgtgc
     3661 gccgcgcggc gggcatcgcc ggcaccgagg tgtcgggcga cggccgtggt ggtggcgaac
     3721 atcaagaaga tgaacaccgt cgatgcgaac gacaccactc gcaccgcgat cgccacgccg
     3781 cccagcgccg tggtgccgag ggtgccgacg atcgcggtgt cgcacagctc ggcgagcggc
     3841 tcggtggcca gcgctagcgc ggcgggaatg gcgatcgcgc cgatctgtcg gtcgatcgag
     3901 gcagacgcgg tcgttggcac gaggcgagcc tacgatgcgg aaattcgcgc agagtgcggg
     3961 tgctcggcgc tcagcgtcgg ggatcgcagg tggctaccat gacgccgtcg gtgaaacggt
     4021 tcgccaggag gtcacggatg cgcaacgacg ggacccgcga acgaggcgcg gcgtcggggg
     4081 cgtgggcagc ggcggcggtt gttctcgcgc tcggagcgtt gttgttcgga ttcaccgcac
     4141 acgttcgcct caacgagatg gaggccgagg tggcccggct gtcgtgggcc accacgacgg
     4201 tgccggtggg cgaaccgcga gtcgccaccg acgaaggcct cggcctcggc gtcggtgccg
     4261 attcggaaca ggcgggttcg gcatcggctg gtacggcgca ggcggggtcg ggggactcgg
     4321 cgcccagcga tgacgatcgc ctggcagtgg tgcgagcgtt cacgaccgtc tacgacgcgt
     4381 cgcagccgct gagcgagcgc ctgttgagcg tcgatgatcc ttccggggtc gacctggcgc
     4441 ttcaagcgat tgaggcgggg ccgaacggcg aactggtgaa acaggtcgtc gtaagcgtca
     4501 acgacgtgaa attcccgacg gcgaactccg ccacggtgat ctacacgatc gtggtgcccg
     4561 gagccgatcc ggtgccgggc cgcaacggtg gggcgaagct catcgacggg gcgtggaagg
     4621 tgaccagggc aaccgtgtgc ggggatctcg ctgcggtcgg aggcagttgc tgaacccgcg
     4681 gccgagcgtc gagcggccgc ctctacgagc ggccggcgtt cagctccaga gccccgactc
     4741 gagggcctcg ggaaccatca ggtgcgaagc tccggcgcga gcggcgctcg atgacacgtc
     4801 gaggtagcga gcctcgagcc gaagggtgac ggcgtcgggt tcggtgatgt ggcgcacacc
     4861 ggcgttgaag gccacatcgg cgcgatcgaa cagggccagg cgcggaagcc gggcgacggc
     4921 gtcgtcgcgg cgtgcgctgt cgccgccgta atacaccgga tcgttcacct gcccccactt
     4981 gtcggcgccc atgatgacga cgtcatagcc ggcagcgatg tcgacgatga ggcgttgctc
     5041 gctgacgacg atgcgcatcc atccgacgcg gcccgccagt cgttcgagca cctcgacacg
     5101 ttgatcgagg ctcggggtgc tgaggtgttc cttgccgatg gggatgcggc tgaccacaag
     5161 gtcgacccgt tcgagttcga gcgtggttcg agcctcctcg gccaccgcga gatgcgcgac
     5221 cgtgggcggg ttgaacgtgc cggggaacac ccccacgcca ccgcggtgcc cactcatgtc
     5281 gcctcgagcc tcgggcctcg ccgagcccag gtcagtgcgg cacaacccgc aacgcacaac
     5341 gtggcggagg tgagcagcgt cgcgttgaac cccaggcgcg tgtagatggc ccctccggcc
     5401 gccgatgcga ctgctcggga caccagcatc acgacgaacg cctgggcgac cgagcgcccg
     5461 cggttgtcga catcgagctc ggtcacgatc ggcagggtgg cgacgaagga cgcctccatg
     5521 gccaggtcca tcagcaacaa catggccacc ccgaacaccg ccgagttgcc gaccgcactg
     5581 ttcgacatgg cgatcaggat caacgacgga atcgccgcca aaccgatcgc gaccccgccg
     5641 acgcggaccg gtccgaggcg gtccgcgtat gcgatcaccc acgccacgcc cacgagctcg
     5701 gccaccccga gggccgcggt ggcaaaggag agttgggtgt tcgacatggc gagatgccct
     5761 gcgaaccagt cgccgttcac ggcgaacacg agcatctgcg aaaacggctg caggcagaaa
     5821 aacgcccaga tggctcggcg aaggcgtgcc gggttggcga tcgcctcgag ggcgtggtgg
     5881 gtggcggcgg agccggtcga ggtcgcggtg gagcccgttg ggcgtgcggc cggtgctccg
     5941 acgggctcgt cctcggcggc accctgctgc gagcgccgaa ggctcgcccc ggaaccg
//
LOCUS       DAOOMA010000081         5949 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_15960_length_5949_cov_4.392603, whole genome shotgun sequence.
ACCESSION   DAOOMA010000081 DAOOMA010000000
VERSION     DAOOMA010000081.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 5949)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5949)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5949
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15960_length_5949_cov_4.392603"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(230..1381)
                     /gene="ispF"
                     /locus_tag="PLP95_03565"
     CDS             complement(230..1381)
                     /gene="ispF"
                     /locus_tag="PLP95_03565"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: protein motif:HMM:NF013308.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HPB44908.1"
                     /translation="MHTDVWTIVVAAGSGQRFGAPKQFELLGDRRVVDHSLAAARRNS
                     DGVVLVTSNDGMDLGNFDADVVVGGGSTRSESVRRGLAAVPSTAEVVLVHDAARPAAG
                     DALFRRVIEAVRSGADAAVPAIAVTDTIRRRGGSTVDRDELVAVQTPQGFRASSLRAA
                     HAGGIDATDDATVVEANGGVVVLVDGESTNLKITERVDLVVAAQQLGVGRGGEHEGSG
                     VEMGLDGLRVGNGFDIHRFSDDPQRALVMGGVVFEGERALHGHSDADVVAHACADALL
                     GAVGLGDIGSHFPDTDERWRGADSMTLLAEVVRMVAAEGKVAINIDCSVIAETPKLAP
                     HKAQMEANLSAVIGAPVSVKGRRAEGIGGLGRREGIAAMATALLMDSDG"
     gene            1520..2179
                     /locus_tag="PLP95_03570"
     CDS             1520..2179
                     /locus_tag="PLP95_03570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012224916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HPB44909.1"
                     /translation="MIDAEKLASIGLFSDLGPDVLDQLLAQSSTLRLVRGDVIFSEGS
                     TADSLYIVESGRIAIGNKSFDGRESMVALMTEGDLFGEMSLFDELGRSAEARALEPSM
                     VVSIPYQPLIDLWRAQPELLWRVVGLLSQRLRNMDEALADSFFLDVTGRTAKHLLEMA
                     GDRDEFEIPITQEELAGLVGASRERVNKAISSFVRLGWLQQNDRTYRIINRRELEIRS
                     M"
     gene            complement(2213..2884)
                     /locus_tag="PLP95_03575"
     CDS             complement(2213..2884)
                     /locus_tag="PLP95_03575"
                     /inference="COORDINATES: protein motif:HMM:NF013866.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HPB44910.1"
                     /translation="MRITLSTGIAAELARPTSGDAVGGLVLWADVFDIRPLFDEMAQR
                     LADAHQWVVVCPELFPGEQHLDVAERLERTAMFVDADKVADAEAAADATGFDRVGVMG
                     FCMGGMWAMKSLASERFERAASFYGMVRLPERWADGTMGDAIDTVRSRDAQGQLKLLA
                     IFGTDDPWCPQSDIDELRTTTSAEVVVYEGAGHGWAQDPARDNYRPEDAADAFTRAEA
                     FLDPV"
     gene            complement(2891..3958)
                     /gene="disA"
                     /locus_tag="PLP95_03580"
     CDS             complement(2891..3958)
                     /gene="disA"
                     /locus_tag="PLP95_03580"
                     /EC_number="2.7.7.85"
                     /inference="COORDINATES: protein motif:HMM:NF010009.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA integrity scanning diadenylate cyclase DisA"
                     /protein_id="HPB44911.1"
                     /translation="MAARRSKALLDALAIVAPGQPLREGLDRVVKASMGALIVVGDGP
                     EVLNICSGGFLLDTAFSAQRLSELAKTDGAIILASDASRIARANVHLVPNPNTPTTET
                     GTRHRTAERVARSIGVPVISVSEDMAVLTVYIHDDKHQLEPIPGLLGRCNQALQTLER
                     YKSRLDEVAANLATAEVEDLVTVRDVVAVLQRTEIVVRIAEEIERYLVELGTDGRLVR
                     LQLRELMAGVEDDRRMVLLDYFEPDATWNLEQAMETLSDLEMEELLEPDAVASALHLG
                     VKDAGGSLSPRGYRMVSKVPRLPGDMAETIVGHFGTLDKLMRATVADLCAVDGVDEQW
                     AVTVKDALGRIAESSILDRYT"
     gene            complement(4036..5409)
                     /gene="radA"
                     /locus_tag="PLP95_03585"
     CDS             complement(4036..5409)
                     /gene="radA"
                     /locus_tag="PLP95_03585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HPB44912.1"
                     /translation="MASSPVLTRNSKKSRFACTECGAVVAKWVGRCDACGEWNTLVEE
                     RMTSSRSSSLAPAMPALPITDVSALDAVPFPTEVAEFDRVLGGGLVPGSVTVLGGEPG
                     VGKSTLLLQVLHGPASGGRTVLYVSAEESAQQVRRRAERLDTLHPSMFLAAETNLTTV
                     LDHIDACSPSVVVIDSVQTVFDPELSSAPGSVGQVRHCAHRLVTVAKERNIAVVLVGH
                     VTKEGALAGPRVLEHIVDTVLSFDGDRHHSLRVLRATKHRFGSTQEVGLFSMGEQGLD
                     AVVDPSALFLADRRPGIEGSIVVPTLEGQRPLVVELQALCVQGAAGNPRRSVSGVDPG
                     RVGLLLAVLDRRLRIPLIALDTYAMAVGGVRVMEPGADLGLGMAMVSSFRNTPLPAGL
                     VAIGEVGLGGELRSVGGIERRLVEAARLGFTTAVVPRSTPALSCGLEVSRVPTIGAAL
                     SLFGLER"
     gene            complement(5497..>5949)
                     /locus_tag="PLP95_03590"
     CDS             complement(5497..>5949)
                     /locus_tag="PLP95_03590"
                     /inference="COORDINATES: protein motif:HMM:NF012861.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HPB44913.1"
                     /translation="GGVVIVGLSIAVQSRQYNGLSLDQLARSLAGGEDVGAGSWLLKL
                     IFTAVTVGSGFLGGEVTPLCVIGATSGVAIASLVGGPIALFAAVGMVATVGSALNAPL
                     ACAAMGVEYFGVGGTAAIVGACLIARVCSGRHSLYSGHHPVPDEASLV"
BASE COUNT         1050 a         2125 c         1825 g          949 t
ORIGIN      
        1 gttcgcgggc agcgggcgcg aaatcgggcc gctccggcgc tccggctccg gccgatccgc
       61 tacgcggcgc accaccgcga cccctccccg gagcgccgga gcggcccgat ttcgcgcccg
      121 ctgcccgcga actgttgcgc ccttggcctc gcgccccacc ggacgctgcg cctcgctttt
      181 tcggtccact catcgtcggc ctctcatcat cgtgtccggc gagtctgcgt tacccgtcgg
      241 aatccatcaa cagcgccgtc gccatcgccg caattccctc gcgtcgaccc agcccgccga
      301 tcccctcggc acggcgcccc ttcaccgata ccggtgcgcc gatcactgcc gacaggttcg
      361 cctccatctg cgccttgtgc ggcgcgagct tgggtgtctc cgcgatcacc gaacagtcga
      421 tgttgatcgc caccttgcct tcggctgcga ccatccgcac gacctcggcc aacagcgtca
      481 tcgaatccgc acctctccag cgctcatcgg tgtcgggaaa gtgcgagccg atgtcgccga
      541 gcccgacggc accgagcagg gcgtcggcgc aggcgtgggc gacgacgtcg gcatcgctgt
      601 ggccgtgcag cgcccgctcc ccttcgaaca cgaccccgcc catcaccaag gcccgctgcg
      661 ggtcgtcgct gaaccgatga atgtcgaatc cgttgccgac ccgaagtccg tcaagtccca
      721 tttccactcc tgaaccttca tgttccccgc cgcggcccac gcccagctgc tgcgccgcga
      781 ccacaagatc gacacgttcg gtgatcttga gattcgtcga ctcaccgtcc accaacacca
      841 cgacaccgcc gttggcctcg accaccgtcg cgtcatcggt ggcgtcgatc ccgcccgcat
      901 gagcggcccg caacgacgac gcacgaaacc cttgcggggt ctgtacggca accagctcgt
      961 cccggtcgac cgttgagcca ccacggcgac ggatcgtgtc ggtcaccgcg atcgccggca
     1021 ccgccgcgtc cgcgccggag cggaccgcct cgatcacccg gcgaaacagc gcatcgccgg
     1081 ccgccggccg tgcagcgtcg tgaaccagca ccacctctgc ggtcgacggc accgcggcga
     1141 gcccacgccg aaccgactcc gaacgcgtcg agccgccgcc gacgacgacg tcggcatcga
     1201 agttgcccag atccatgccg tcgtttgagg tcaccaacac cacgccgtcg ctgtttcgtc
     1261 gcgccgcggc aagcgagtga tccaccaccc gccggtctcc gaggagctcg aactgcttcg
     1321 gtgcgccgaa tcgctgaccg ctcccggcgg cgacgacgat ggtccacacg tcggtatgca
     1381 tgaccggcac agcctaccgg ccgagcgtcg ccccgttctg cggccaccgc cgagtcgacc
     1441 tccccggcat tgcgccgatc gtgtgacgaa tttgactcga tgagtccccg aacccggcga
     1501 atgtcgatac cgtggatgcg tgatcgacgc tgaaaaactg gcatccatcg gactgttctc
     1561 cgatctcgga cccgatgttc tcgaccaatt gctcgcccaa tcctccaccc tccgtctggt
     1621 tcgtggagat gtgatctttt ccgagggcag caccgctgat tcgctctaca tcgtcgaatc
     1681 cggacgcatc gccatcggca acaaatcctt cgacggccgc gaatccatgg ttgcactcat
     1741 gaccgaaggc gacctcttcg gcgagatgag cctgttcgac gaactcggcc gctccgccga
     1801 agcccgggcg ctggaaccct cgatggtcgt atcgattccc taccagccgt tgatcgacct
     1861 gtggcgtgct cagcccgagt tgttgtggcg ggtggtcggg ctgttgtcgc agcggctccg
     1921 caacatggac gaagcgctgg cagattcctt cttcctcgat gtcaccggcc gcaccgccaa
     1981 gcacctgctc gagatggccg gcgaccgcga cgagttcgag attccgatca cccaggagga
     2041 actcgccggc ctggtcggag catcgcgcga gcgggtcaac aaggcgatct cgagcttcgt
     2101 tcggctcggt tggttgcagc agaacgaccg cacctaccgc atcatcaatc gacgggaact
     2161 cgaaatccgc tcgatgtagc gcgtcgacgg cgaacttcgc gaccccagcg tctcagaccg
     2221 gatcaagaaa cgcctctgcc cgggtgaacg cgtcggccgc atcctcgggt cgatagttgt
     2281 cgcgggccgg gtcttgtgcc cacccgtgtc cggcgccctc gtacacgacg acctcggccg
     2341 aggtcgtcgt gcgcaactcg tcgatatcgg attgcgggca ccacggatcg tcggtgccga
     2401 agatcgccag caacttcagt tgtccctgcg cgtcccggct ccgcaccgtg tcgatcgcgt
     2461 cgcccatggt gccgtcggcc cacctctccg gaaggcgcac catcccgtag aacgaagccg
     2521 ctcgttcgaa ccgctccgac gcgagactct tcatcgccca catcccgccc atgcagaacc
     2581 ccatgacgcc gacacggtcg aacccggttg catcagcggc ggcctcggcg tcggccacct
     2641 tgtcggcgtc gacgaacatg gcggtgcgtt cgaggcgctc ggcgacgtcg aggtgctgtt
     2701 cgccggggaa caactccggg cacacgacga cccactggtg cgcatcggcg agacgttggg
     2761 ccatctcgtc aaagagcggc cggatatcga acacatctgc ccacagcacc aggccaccca
     2821 ccgcgtcacc gctcgtcggg cgggcgagtt ccgcagcgat tccggtggac agggtgattc
     2881 tcataggacc tcaggtgtag cgatcgagga tcgacgactc ggcgattctg ccgagcgcgt
     2941 ctttcacggt gacggcccac tgctcgtcca ccccgtcgac ggcgcacagg tcggccaccg
     3001 tcgctcgcat gagtttgtcg agggttccga agtgaccgac gatggtctcg gccatgtctc
     3061 cggggaggcg gggcaccttg gacaccatgc ggtatccccg gggtgacagg ctgccaccgg
     3121 cgtctttcac cccgaggtgc agcgcactcg ccaccgcgtc gggttcgagg agttcttcca
     3181 tctcgagatc cgacagcgtt tccatcgcct gttcgagatt ccaggtggcg tcgggctcga
     3241 agtagtcgag cagcaccatt cgccgatcgt cttccactcc cgccatcaac tcgcgcaact
     3301 gcagccggac gaggcggccg tcggttccga gttcgaccag gtaccgctcg atctcctcgg
     3361 cgattcgcac gacgatttcg gtgcgctgca gcaccgcgac gacatcacga accgtcacga
     3421 gatcttccac ctcggcggtt gcgaggttgg cggccacctc gtcgaggcgg ctcttgtagc
     3481 gctcgagcgt ctgaagcgcc tggttgcaac gaccgagcag ccctggaatc ggttcgagtt
     3541 ggtgcttgtc gtcgtggatg tacacggtca gcaccgccat gtcctcggac accgaaatca
     3601 cgggaacccc gatcgaacgg gcgacgcgct cggcggtgcg gtggcgagtg ccggtttcgg
     3661 tggtcggagt attcggattg ggaacgaggt gcacattcgc tcgggcaatc cggctggcgt
     3721 cggaagccaa gatgatggcc ccgtcggtct ttgccaactc cgacaatcgc tgggcgctga
     3781 acgccgtgtc gagcaggaac ccgcctgaac agatattgag gacctctggc ccgtctccga
     3841 ccacgatgag cgcgcccatg gacgccttca ccactcgatc caggccttcg cgcaacggtt
     3901 ggcccggtgc cacgatggcg agagcgtcga gaagagcttt gcttcgacgg gctgccatgg
     3961 cggcagtcta gtggcgcgac ccagccgcat cgggcgtggg tccgacatgc ggggcagtgg
     4021 cggctgtaac cgggatcatc gttccaaccc gaacagcgag agcgccgccc cgatcgtcgg
     4081 cacccgcgac acctcgagcc cgcacgacaa cgcaggggtt gagcggggca ccaccgcggt
     4141 ggtgaaccca aggcgtgccg cctcgaccaa gcgccgctcg atgccgccca ccgaacgcaa
     4201 ttcgccaccg agaccgacct cgccgatggc gacaagccct gccggcaacg gggtgttgcg
     4261 aaacgacgag accatcgcca tgcccaagcc caggtcggct cccggctcca tcacccgaac
     4321 gccaccgacc gccatcgcgt aggtgtcgag cgcgatcaac ggaatccgga gtcgacgatc
     4381 gagcaccgcc aacagcaacc ccacccggcc aggatcgacc ccgctcaccg atcgacgagg
     4441 attccccgcc gccccttgga cacacagcgc ctgcaactcg accacgagtg ggcgctgccc
     4501 ttcgagggtc ggcaccacga ttgaaccctc gattccgggg cgtcgatcgg cgaggaacaa
     4561 cgcggatggg tcgacgacgg catcgaggcc ctgctcgccc atcgagaaca ggcccacctc
     4621 ctgggtggat ccgaagcggt gcttggtggc gcgaagcacc cgcagcgagt gatgccgatc
     4681 tccgtcgaaa ctcagcacgg tatcgacgat gtgttcgagg acgcgcggcc ccgccaacgc
     4741 gccctccttc gtgacatggc ccaccagtac cacagcgatg tttcgctcct tcgcaacggt
     4801 cacgagacgg tgtgcgcaat ggcggacctg ccccaccgat ccgggggcgg aggacaattc
     4861 ggggtcgaac acggtttgca ccgagtcgat gacgacgacc gacggcgaac aggcgtcgat
     4921 atggtcgagc acggtcgtca agttcgtttc ggcggcgagg aacatcgagg gatgcagcgt
     4981 gtccaaccgc tcggcgcgac gtcggacctg ctgggccgac tcctccgccg agacgtacaa
     5041 caccgtgcga ccaccgctcg cggggccgtg cagaacctgc aacagcaacg tcgacttgcc
     5101 gaccccgggt tcgcctccca acaccgtgac cgatccgggg accaacccac cgccaaggac
     5161 gcggtcgaac tcggcgacct cggtgggaaa ggggaccgca tcgagtgcgc tgacgtcggt
     5221 gatgggcagg gccggcatcg ccggagccag cgaggacgac cgactcgagg tcattcgctc
     5281 ttcgacgagc gtgttccatt caccgcacgc gtcgcagcgg ccgacccact ttgcgaccac
     5341 ggcaccacac tcggtgcagg cgaagcggga cttcttggag ttgcgggtga ggaccgggct
     5401 cgatgccatg tgagagaggt tagaaacgac ccctgacact cgaggttcga ggtgtccgat
     5461 cgacggccgc cacggagcgc cagtcatcgg gcaggctcac accaacgagg cctcgtcggg
     5521 caccgggtgg tggcccgaat acaacgagtg gcggcccgaa cacacccggg caatcaggca
     5581 cgcgccgacg atcgccgcgg tcccaccgac cccgaagtac tccactccca tcgccgcaca
     5641 ggcgagcggc gcgttgagcg ccgaaccgac ggttgcgacc attccgaccg cggcgaacaa
     5701 cgcgatcgga ccgccgacca acgaggcgat ggcgacaccg gaggttgcgc cgatcacaca
     5761 cagcggggtg acctcaccac cgagaaatcc cgatccgacc gtgaccgcgg tgaagatcag
     5821 tttgagcagc cacgaccctg cgcccacgtc ctcgccaccg gccagcgagc gggccaactg
     5881 gtcgagcgac agtccgttgt actgccggct ttggaccgcg atgctcaatc cgacgatgac
     5941 gacccctcc
//
LOCUS       DAOOMA010000082         5906 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_16138_length_5906_cov_3.937276, whole genome shotgun sequence.
ACCESSION   DAOOMA010000082 DAOOMA010000000
VERSION     DAOOMA010000082.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 5906)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5906)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5906
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16138_length_5906_cov_3.937276"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..516)
                     /locus_tag="PLP95_03595"
     CDS             complement(<1..516)
                     /locus_tag="PLP95_03595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB44914.1"
                     /translation="MSGRRVGREPTGHIAASDCERCRRGLVAQPVNTVSSLAFVAAGA
                     LTIRSLRRRRVDTPGEAAVGWAAMAAGLGSVAYHGPGTAFGRYVHDASLLNLLASLTL
                     ADGSRASGRPVPRSALAIVPVVSSVAAHPRTSMGAQAAFGIAAGAAEAARVATEDSSR
                     AIRVRDAAEAVT"
     gene            complement(513..1943)
                     /locus_tag="PLP95_03600"
     CDS             complement(513..1943)
                     /locus_tag="PLP95_03600"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HPB44915.1"
                     /translation="MTQHALDPDATAFATRRVTLRDRIITIVAGLLTRAFFRSVEVEG
                     SPPPPGPVVLAASHLYGFVDPVLLVAELGILPRFLAKATLWDTAVARPLLGFARVIPV
                     HRRADGASGDDNASMFADAVAALEGGSMLALFPEGTTHDDPSIRPLRTGAARIAIQAA
                     GSGVQGVSIVPVGVTYEDKVAVRGRAIVSYGTPIEVPSGALLGPDGEPLHEQVRELTD
                     HLQREIQALTPHFDTTEEALALGAAATMTLHEERAAQTAVPLAEVNSTARRLALVEPA
                     QRNALVSLVARYRMLLGFVGIDDVDVQRGISLTALARKIAVIAVVVTFLSPVALAGLL
                     ANIVPVALVLIAGLIPKAPVSKGTIRVLVAAVAFPATWLTLAFADARPGAFGDLLRSI
                     TLPVETLLGSAHHDRLGAVANLVVLVAVPLMGVLAMFVVERIRVLVDTIVRWRTFLDR
                     RGQMDEVRERRREVVELTRRMLEQAS"
     gene            2041..3420
                     /locus_tag="PLP95_03605"
     CDS             2041..3420
                     /locus_tag="PLP95_03605"
                     /inference="COORDINATES: protein motif:HMM:NF021523.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2252 domain-containing protein"
                     /protein_id="HPB44916.1"
                     /translation="MNEDLHGRAVDDAAHRRADLSEGRAARKRAPRKGLGVWDPAARQ
                     APAIERLRAQEPVRDQDLLPLRYARMASSPWAYLRGAAAVMASDLATRPHSGLTVQMC
                     GDAHVLNFGLWASPERRLLFNVRDFDETLPGPFEWDLKRLGASVFVLADSVGLPESRS
                     AAAVTAAVEGYCEAIGRYAEMGELEVWYDRIDADAVRDELAPDAIEKVERVVRKQARK
                     RSQAGAVQQFTEVVNGRRRIIEELPKRQRVAPNSIGAAGGFEEVYSMYLASIPAHLER
                     LISRFKRLDAVRQVVGVGSVGMRVWLLLMEGDSGVPVFFQIKQATHSVFEDVLEPSRF
                     ASHGERVVVGQRLMQSASDMFLGHLSAGGHDYYLRQFRDMKVIPDGDRIAGSLTEFSR
                     LCGLALAKSHARSGEPGDIADYIGKGAAFCRSIVDFSRAYAAQTHADHADLVALAAAG
                     GIEVAERPW"
     gene            complement(3431..4444)
                     /locus_tag="PLP95_03610"
     CDS             complement(3431..4444)
                     /locus_tag="PLP95_03610"
                     /inference="COORDINATES: protein motif:HMM:NF012568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HPB44917.1"
                     /translation="MALSNPLDIMPTMAADLARVELELAASASSDDPYLTEIASHLIS
                     AGGKRVRPGFCIAAAATSPNDPSFGAGASGAHIDIVRAAVSVELVHLGSLYHDDVMDD
                     AIIRRNVESVNAKWGNLKAILAGDFLLARASEIASSLGVEVAGLLASTIARLCEGQVL
                     ELRYTYDTFRSEADYLSSIDGKTAALLATACRIGGIVAELPDAQIDALTTFGSAYGMA
                     FQIVDDVLDLVATPEELGKPSGHDMLEGVYTLPVLRTLQSDSGPELRPLLTDSMSDAD
                     LATAVALVKEAGWIHGAIDVARSYANDAVSAIGSLPESMGVVGLQAATAHLVDSVESL
                     AAR"
     gene            4672..>5906
                     /locus_tag="PLP95_03615"
     CDS             4672..>5906
                     /locus_tag="PLP95_03615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease N-terminal domain-containing
                     protein"
                     /protein_id="HPB44918.1"
                     /translation="MFERFTDRARRVVVLAQEEARLLNHNYIGTEHILLGLIHEGEGV
                     AAKALESLAISLEAVRQQVEEIIGQGGSSPSGHIPFTPRAKKVLELSLREALQLGHNY
                     IGTEHILLGLIREGEGVAAQVLVKLGADLSRVRQQVIQLLSGYPGPAGASGQSGQPQQ
                     GGEKASTGGGSGDNASGSLVLDQFGRNFTQLARDKKLDPVIGREKETERMMQVLSRRT
                     KNNPVLVGEPGVGKTAIVEGLAQAIAAGNVPETLLNKQLYTLDLGALVAGSRYRGDFE
                     ERLKKVLKEIKTRGDIILFIDEIHTLVGAGAAEGAIDAASILKPMLARGELQTIGATT
                     LEEYRKYLEKDAALERRFQKIVVEEPSVAHTIEILKGLRERYENHHRVTITDQALVAA
                     ANLADRYIPDRFLPDKAIDL"
BASE COUNT         1006 a         1937 c         2027 g          936 t
ORIGIN      
        1 ggtcacggcc tcggccgcgt ccctcacccg gatcgcccga ctcgagtcct cggtcgcaac
       61 ccgggccgcc tccgccgccc ccgccgcgat gccgaacgcc gcctgtgctc ccatggacgt
      121 tcgcgggtgg gccgccaccg aggacacgac cggcacgatc gcgagtgccg acctcggtac
      181 cggccgaccc gaagcacgcg agccatcggc caaggtcaac gaagcgagca ggttcagcag
      241 tgacgcatcg tggacgtatc tgccgaacgc ggtgccgggc ccgtggtacg cgacgcttcc
      301 gagccctgcc gccatcgcgg cccaccccac ggcggcttcg cccggagtgt cgacccggcg
      361 ccgccgcagc gatcgaatgg taagcgcgcc cgcggcgacg aacgcgaggc tggacacggt
      421 gttgaccggc tgagcgacga ggccccgacg acaccgctcg caatccgatg ccgcgatgtg
      481 gccggtgggc tcgcggccga ctcggcgccc gctcacgacg cctgctcgag catgcggcga
      541 gtgagctcca ccacctcgcg acgtcgctcg cgcacctcgt ccatctggcc gcgacggtcg
      601 aggaacgtcc tccaacgaac gatggtgtcg accaggacgc ggatccgctc gacgacgaac
      661 atcgcgagaa cgcccatcag cgggacggcc accaacacga ccaggttcgc caccgcgccg
      721 agccggtcgt gatgagcgga acccaacagg gtctccaccg gcaaggtgat actgcgcaac
      781 aggtcgccga acgctccggg tcgggcgtcg gcgaaggcca gcgtgagcca ggtcgccgga
      841 aacgccaccg cggcgacgag aacgcggatg gtgcccttcg aaaccggagc tttcgggatg
      901 agcccggcga tcaacaccag ggccaccggc acgatgttcg ccagcaggcc ggcgagtgcc
      961 acgggagaca ggaacgtcac gacgacggcg atcaccgcaa tctttcgggc gagcgcggtc
     1021 aggctgattc cgcgctgcac gtcgacatca tcgatgccga cgaacccgag cagcattctg
     1081 taccgagcca ccagcgacac caacgcattg cgctgcgccg gctcgacgag ggccaaacgg
     1141 cgggcggtcg aattcacctc ggcaagcggc accgctgtct gtgcggcgcg ttcctcgtgc
     1201 aacgtcatgg ttgccgcggc cccgagggcg agtgcctcct cggtggtatc gaagtgcggg
     1261 gtcaatgcct gaatctcgcg ctggagatgg tcggtcagct cgcgcacctg ctcgtggagg
     1321 ggttccccgt cgggtccgag cagggcgccg ctcgggacct cgatcggtgt tccgtagctg
     1381 acgatcgccc gtccgcgaac cgccaccttg tcttcgtagg tgacccccac cggcacgatg
     1441 ctcactccct gcacccctga ccccgcagcc tggatcgcaa tgcgggcggc tccggtgcgc
     1501 agcggtcgaa tcgacggatc gtcgtgggtg gtgccttcgg ggaagagcgc gagcatcgac
     1561 ccgccttcga gtgcggccac cgcgtcggcg aacatcgatg cgttgtcgtc cccacttgcg
     1621 ccgtcggccc gccgatgcac ggggatgacg cgggcgaacc ccagcaacgg cctcgcgact
     1681 gcggtgtccc acagcgttgc cttggccagg aaacgaggca ggattccgag ctccgccacc
     1741 aacagaacgg gatcgacgaa gccgtacaga tggctggcgg cgagcacgac cgggccgggc
     1801 ggtggtggcg acccttcgac ttcgacggaa cggaagaacg ctcgcgtgag caacccggcg
     1861 acaatcgtga tgatgcgatc tcgcagcgtg acgcgccgag tcgcgaacgc cgtggcgtca
     1921 gggtccaagg cgtgctgtgt caccggctcc tcctcgctcg tggtcatcgg cctttgtccg
     1981 gtgaacgtct gtggatgcta ccggcgagcg ttggtggcgg cgatgcgtca cgctgttgcc
     2041 atgaacgaag atctccatgg ccgggccgtc gacgacgcgg cccatcgacg ggcggatctg
     2101 tccgagggtc gggcggcgcg aaagcgggcg ccgcgcaagg ggcttggcgt gtgggacccg
     2161 gccgcccggc aggcaccggc gatcgaacgc ctccgcgccc aggagccggt acgggatcag
     2221 gacctgctgc cgcttcgcta tgcgcggatg gcctcgtcgc cgtgggcgta tctgcgcggt
     2281 gccgccgcgg tcatggcctc ggatctggcg acccgaccgc actcggggtt gacggtgcag
     2341 atgtgtggtg atgcacacgt gctcaacttc ggtctgtggg cctcaccgga acgacggttg
     2401 ttgttcaacg tccgcgactt cgatgagacg ctgccgggcc cgttcgagtg ggatctcaag
     2461 cgtctcggcg cgagcgtgtt cgtgctcgcc gattcggtcg gcctgcccga atccaggagt
     2521 gcggcagcgg tcaccgccgc ggttgagggc tactgcgagg caatcggccg ttacgccgag
     2581 atgggggagt tggaggtctg gtatgaccgg atcgatgccg acgccgtccg cgacgaactg
     2641 gctccggatg ctatcgagaa ggtcgagcga gtcgttcgta aacaggcgcg caagcggagc
     2701 caggccgggg cggtacagca gttcaccgaa gtggtgaacg ggcgccgccg catcatcgag
     2761 gagctaccga agcgccaacg cgtcgcgccg aactcgatcg gtgccgccgg tgggtttgag
     2821 gaggtgtatt cgatgtatct ggcgtcgata ccggctcatc tggaacgtct gatttcccgg
     2881 ttcaaacgcc tcgatgccgt tcggcaggtg gtcggggtcg gcagcgtcgg aatgcgggtc
     2941 tggttgttgc tcatggaggg cgacagcggg gttccggtgt tctttcaaat caaacaagcc
     3001 acgcactcgg tgtttgagga tgtgctcgaa ccgagccgat tcgcgagcca cggcgagcgg
     3061 gtggtggtcg gtcagcggtt gatgcagtcc gccagcgaca tgttcctggg gcacctcagc
     3121 gcaggtggcc acgactacta cctccggcag ttccgcgaca tgaaggtcat tcccgacggc
     3181 gaccggattg ccgggtcgct cacggagttc agccgtctgt gcggcctggc gttggccaag
     3241 tcgcacgcgc gcagcggcga acccggcgat atcgccgact acatcggaaa gggcgcggcc
     3301 ttttgtcgct cgatcgtcga cttcagccgg gcctatgccg cccagaccca tgccgaccat
     3361 gccgacctcg tcgcgctcgc ggccgccggc ggaatcgagg tcgccgaacg gccgtggtag
     3421 cgcttggacc tcagcgcgcg gcgagcgact cgaccgagtc gacgaggtgg gcggtcgcgg
     3481 cctgcaatcc cacgaccccc atcgactccg gcagtgaccc gatcgccgac accgcatcgt
     3541 tggcgtacga gcgagcgacg tcgatggcgc cgtgaatcca accggcctcc ttcaccagcg
     3601 cgacggcggt ggcgaggtcg gcgtcgctca tcgagtcggt caggagtggc cgcaactccg
     3661 gaccgctgtc gctctgcagg gtccgcaaca ccggcagggt gtagacgccc tccaacatgt
     3721 cgtggccgga gggtttgccg agctcctcgg gggtcgccac gaggtccaac acgtcgtcga
     3781 cgatctgaaa cgccatgccg tatgccgagc cgaacgtggt gagcgcgtcg atctgggcgt
     3841 cgggcaactc ggcgacgatg cccccgattc gacaggcggt cgccaggagg gccgcggtct
     3901 tgccgtcgat cgaggagagg tagtcggcct cggagcggaa cgtgtcgtag gtgtaacgca
     3961 gttcgaggac ttgaccctca cacagacggg cgatggtgct ggccaacagc ccggcgacct
     4021 cgactccgag cgaactcgcg atctccgacg ctcgggcgag caggaaatcg ccggcgagga
     4081 tggccttgag gttgccccac ttggcgttca ccgattcgac gttgcgccgg atgatcgcat
     4141 cgtccatcac gtcgtcgtgg tacagcgagc cgaggtgcac caattcgacc gacaccgcgg
     4201 cgcgcacgat gtcgatgtgt gcccccgatg cgccggcgcc gaacgacggg tcgttgggcg
     4261 aggtggcggc cgcggcgatg caaaagcccg gacgaacccg ctttccgccg gcggagatca
     4321 ggtgcgaggc gatctcggtg agataggggt catcggagct cgccgaggca gcgagttcga
     4381 gttccacgcg tgcgaggtcg gcagccatgg tcggcatgat gtcgagaggg ttgctcaaag
     4441 ccacggcgcc caaggtagtt ggtctggcgc gcgttgcccc atgcgagggg ctgtgcccca
     4501 cgtctcgtgt ggaggcgggg ctgagtcggg cctcggctga gaacttcttg ggtcaaccga
     4561 cccgggtcgg aagaatcgcg gcgaggtgat tgttgaacca cttgccacgc ccgcaccttt
     4621 gaatgtcagg gtccccgggt agactggcgg agagtccgca ggaggcactc tgtgtttgaa
     4681 cgatttaccg accgagcccg ccgcgtcgta gtcctcgcgc aagaagaggc gcgcttgctc
     4741 aaccacaact acatcggcac cgagcacatc ctgctcggcc tgatccacga gggtgagggc
     4801 gtcgctgcca aggcgcttga atcgctggcg atttcgcttg aggccgtgcg ccagcaggtg
     4861 gaggagatca tcggccaggg cggttcgtcg ccgtctgggc acatcccctt caccccgcgt
     4921 gccaagaagg tgctcgagtt gtcgctgcga gaggcgttgc agctcggcca caactacatc
     4981 ggcaccgagc acatcctgct cggtctcatc cgcgagggtg agggcgttgc ggcacaggtg
     5041 ttggtgaaac tcggcgccga cctgtcgcgg gtgcgccagc aggtgatcca gttgctgagc
     5101 ggttatcccg gcccggccgg cgcttccggt caatccggcc agccgcaaca gggtggggag
     5161 aaggcgtcga ccggtggcgg ttcgggcgac aacgcgtccg gttcgctcgt gctcgatcag
     5221 ttcggccgca acttcaccca gctcgctcgc gacaagaagc tcgacccggt catcgggcgc
     5281 gagaaggaaa ccgaacggat gatgcaggtg ctcagccgcc gcaccaagaa caatcccgtt
     5341 ctggtcggcg agccgggtgt cggcaagacc gccatcgtcg agggcctcgc ccaggcgatc
     5401 gcggccggca acgtgcccga gacgctgctc aacaagcagc tgtacaccct cgatctgggt
     5461 gccctcgtcg ccggttctcg ctatcgcggc gatttcgagg agcgcctcaa gaaggtgctg
     5521 aaggaaatca agacccgcgg cgacatcatc ttgttcatcg acgagatcca caccctcgtc
     5581 ggtgcgggcg ccgcggaagg cgcgatcgat gctgccagca tcctcaagcc gatgctggcc
     5641 cgtggcgagt tgcagaccat cggtgcgacc acactcgagg aataccgcaa gtacctcgaa
     5701 aaagatgcgg cactcgagcg acgcttccag aagatcgtgg tggaggagcc ctcggtggct
     5761 cacaccatcg agatcctcaa gggcctgcgc gaacgctacg agaaccacca ccgcgtgacg
     5821 atcaccgacc aggcgctggt cgcggcggcc aacctggccg accgctacat cccggaccgc
     5881 ttccttcctg acaaggcgat cgacct
//
LOCUS       DAOOMA010000083         5867 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_16300_length_5867_cov_4.493462, whole genome shotgun sequence.
ACCESSION   DAOOMA010000083 DAOOMA010000000
VERSION     DAOOMA010000083.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 5867)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5867)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5867
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16300_length_5867_cov_4.493462"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..704)
                     /locus_tag="PLP95_03620"
     CDS             complement(<1..704)
                     /locus_tag="PLP95_03620"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HPB44919.1"
                     /translation="MKVLVLGGGSDIAAATVTRLARIAGNGRLSVVLAARDVDAASAT
                     MSSVAPGVNVVARRWDATQVARHQAFIDDVFDRHGPFDLVLCAVGMLGHHAGRGVGPV
                     EVDLMIRTNFSGPAAALSAVADRMAARRHGSIVVLSSIAGARARRSNYVYGSSKAGLD
                     SFAQGLGDALVGTGVEVIVVRPGFVRSKMTDGLDPAPFSRRPGDVAEAVIGALGGGST
                     IVWVPHRRALRSPPRPR"
     gene            complement(701..1336)
                     /locus_tag="PLP95_03625"
     CDS             complement(701..1336)
                     /locus_tag="PLP95_03625"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HPB44920.1"
                     /translation="MSRPRVVVVDDHALFRAGVVAELGDRVELVATADDIDAAIAVVC
                     ETLPDVVLLDVHLPSGTAEAVITACAHLDTVRFLALSVSDAPDDVITLVRAGARGYVT
                     KSISSEELCDAVDRVAAGDAVFSPRLAGFVLDAFHRSADNAVDPDLDQLTNREREVLR
                     LLARGYAYKEIARELMISVKTVETHASAVLRKLQLSNRSELSRWASERRLI"
     gene            complement(1333..2553)
                     /locus_tag="PLP95_03630"
     CDS             complement(1333..2553)
                     /locus_tag="PLP95_03630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB44921.1"
                     /translation="MSVFEPNPLADTPAHSDAVIWGICGQTAACWGVSVVVVRAAIVV
                     LAVAGGFGVVVYAALGVWLRLRAERAPGRREPSAGRDLGVAVLTAGVVWELGRWWPGV
                     RPELVVPVAFVAMGIAVGWRAPAVGDRPGAGAAVGDRTGAGAQTSRSAGFGQLAMRVF
                     GGAAMTLAGLAVVFGQRASLSELRDTGLALAVALAGVGVVMGPAAVRLVRGLGAERDE
                     RIRGQERAAVAAHLHDSVLQTLTLIQKRSEDPATMAALARQQERSLRRWLYGGGEVVA
                     SLEDIGWRGRAERIAAEIEDAYYVAVELVMVGEGESSPAIDAALLASREALVNAAKFS
                     GVADISMYCERTEGRFDVYIHDRGAGFDLDAVAPDRRGISESMIGRMERVGGRATITP
                     VVGEGTEVHLEVEE"
     gene            2686..4593
                     /locus_tag="PLP95_03635"
     CDS             2686..4593
                     /locus_tag="PLP95_03635"
                     /inference="COORDINATES: protein motif:HMM:NF015957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0080135 - regulation of cellular response
                     to stress [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HPB44922.1"
                     /translation="MTTTPPPTPPDISASPATDPPPDLPPDLPPDLPPEQPPEQPTGS
                     ATDGPSDGPSDSSPDSPADSLTGGDRSDLRRWWDSLYRIDTNRILGGVCGGLGHRFGM
                     PRWLVILGLVLIIMLGPGVPLYLGLWLLLPDEYGRRILQDSRWRDLAIAALGVVVAFT
                     ALGLGGNLFFLKELGTVAPWAVILVGIGLLTRRPGAPTASPPQSTSAHLSEAQAVSTT
                     MASTATAAATPPKATTLRGSGEPRKRRTYRTPVIGPFTWCVALIAAALLGLVALGDPR
                     SIGPGVIVAAVTIVFGAGLVFSAFRGRARGLVLPALAGICLLLGLGVIDLRADTFLSA
                     VDVRYVTQEELPRRVESGWGRTRVDLSTLELQSDTSIDIEAPAGITTVVLPAAANSEV
                     HVDIGAGDARRYGALPMNDDLRRWATSSPLPSTGDPIDTDRASRLSDQYAVAEVAIAT
                     RDSTMSVDTGSDHTLVVNISLGAGEVRVIEPVWASVPTSVMTPQRACLAAVGADAEIG
                     ATAEGGATAEGGATSEAVTDDEMVAVPCTSVEDPASLVPACYDRFTGWLADCAGPRDQ
                     WTDVVPACFNWETNIVTCEEVGVSTGLGFSSGATPPTDAVPQTEVDPNPASVPDTSLD
                     ATGGTQPEGTN"
     gene            4593..4820
                     /locus_tag="PLP95_03640"
     CDS             4593..4820
                     /locus_tag="PLP95_03640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB44923.1"
                     /translation="MKRHPLDAIALIAGLLTIGFGLLGLAHQLGAFPLGPGGVALAIC
                     LAVGFGGIGLLVLAPKTRPDVSDPRLSKRGR"
     gene            4862..5740
                     /locus_tag="PLP95_03645"
     CDS             4862..5740
                     /locus_tag="PLP95_03645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HPB44924.1"
                     /translation="MADSSTLPARVEFTVTDEGPGTETMTLVGDAHGNPSGQPVVLLH
                     GGGQTRHSWGNTAASIAHDGWRAYAVDMRGHGESGYSPSGAYTASRFGHDLVAIASHL
                     DSKPVVVGASLGGLAGLLAAGSYSPDSFAALVLVDITPRPETVGVNRVVGFMLDRAEE
                     GFATLDEAADAVGRYNPHRPRPTDLSGLRKNLRLGEDGRWRWHWDPALFNPQNGLAGV
                     QEPGQFQRAAEALAIPTLLVRGRQSDLVSPESAQEFLATVPHAEFVDVSGAGHMVAGD
                     RNDRFCDAVSEFLSRR"
BASE COUNT          930 a         2175 c         1867 g          895 t
ORIGIN      
        1 cgtggccgag gcggtgatcg gagcgctcgg cggtgcggca cccacacgat cgtgctccca
       61 ccgccgagcg ctccgatcac cgcctcggcc acgtcgcctg ggcggcgcga gaacggggcc
      121 gggtcgagcc catcggtcat cttcgagcgg acgaagcccg ggcgtacgac gatcacctcg
      181 accccggtgc ccaccagtgc gtcgccgagg ccctgagcga agctgtcgag cccggccttc
      241 gagctgccgt acacgtagtt cgagcgcctc gccctcgcac cggcgatcga gctcagcacc
      301 acgatggagc cgtgacgacg tgccgccatg cggtcggcca cggcagacaa cgcagccgcc
      361 gggcccgaga agttcgtgcg tatcatcagg tcgacctcga ccgggccgac gcctcgtccg
      421 gcatggtggc cgagcatccc cacggcgcac aggacgaggt cgaagggacc gtggcggtcg
      481 aagacatcgt cgatgaacgc ctgatgacgc gccacctggg tagcgtccca acgccgcgcg
      541 acgacgttga ccccgggcgc gacgctactc atcgtcgcac tcgcggcatc gacgtcgcga
      601 gccgcgagca ccaccgacag cctcccgttt ccggcgatgc gcgccaggcg cgtcaccgtc
      661 gccgcggcga tgtcgctgcc gccgccgagc accagcacct tcatatcagc ctccgttccg
      721 acgcccaccg gctcagctcc gagcggttcg acaactggag cttgcgcagc accgccgacg
      781 cgtgcgtctc gaccgtcttg acgctgatca tcagctcgcg cgcgatctcc ttgtacgcat
      841 agccacgtgc gagcagccgc aaaacctcgc gctcgcgatt cgtcagttga tcgaggtccg
      901 gatcgacggc gttgtcggcg ctacggtgga acgcgtcgag cacgaacccc gcgagtcgtg
      961 gcgagaagac ggcgtcgccc gccgcgaccc tgtcgaccgc atcgcacagc tcctcacttg
     1021 agatcgactt ggtcacgtaa ccccgggcgc cggcacgcac gagcgtgatc acgtcgtcgg
     1081 gggcgtcgga aacgctgagg gcgagaaacc ggaccgtgtc gaggtgggcg cacgcggtga
     1141 tcacggcctc tgccgttccc gacgggagat ggacatcgag caacacgacg tccggcaggg
     1201 tctcacacac cacggcgatc gcggcgtcga tgtcatcggc ggtggccacg agttcgaccc
     1261 gatcgcccag ttcggcgacc accccggcgc gaaacaacgc gtggtcgtcc accaccacga
     1321 ctcggggcct gctcattcct ccacctcgag gtgcacctca gtcccttcac caaccaccgg
     1381 cgtgatcgtc gcccgtccgc cgacccgttc cattcgcccg atcatcgact cgctgattcc
     1441 acgacggtcg ggagctaccg cgtcgagatc gaatcccgca ccgcggtcat gaatgtagac
     1501 gtcgaaacgt ccttccgttc gctcacagta catcgagatg tcggccactc cggagaattt
     1561 ggcggcgttg acgagcgctt ctcgcgatgc caggagcgcg gcatcgatcg ccgggctgct
     1621 ctcaccttcg cccaccatca ccaactcgac agcgacgtag taggcgtcct cgatctcggc
     1681 agcgatccgc tcggcccgac cccgccaccc gatgtcctcc agcgacgcca cgacctcgcc
     1741 gccgccgtac agccaacgcc gcagcgagcg ctcctgttgg cgggccagcg ccgccatcgt
     1801 cgcaggatcc tcggagcgct tctggatgag cgtgagcgtc tgcagcaccg aatcgtgaag
     1861 gtgagcggcc accgcggcgc gctcctggcc gcgtatccgc tcgtcgcgct cggccccgag
     1921 tccgcggacg agtcgcaccg ccgccggacc catgacgacg ccgacgccgg cgagcgcaac
     1981 ggcgagcgcc agccccgtgt cgcgcagttc cgacagtgac gcccgttggc cgaacacgac
     2041 ggccagacca gcgagggtca tcgccgcacc gccgaacacc cgcatggcga gctggccgaa
     2101 cccggccgaa cgcgacgtct gcgcaccggc gccagtgcgg tcgccgacgg cagcgccagc
     2161 accggggcgg tcgccgacgg ccggtgcacg ccatccgacc gcaattccca tggcgacgaa
     2221 tgcgaccggt accaccaatt cggggcgaac gccgggccac caccgcccga gttcccacac
     2281 caccccggcc gtgagcaccg ccaccccgag gtcgcgtccc gcactcggct cgcgtcgccc
     2341 gggcgcccgc tcggcgcgca ggcgaagcca cacaccgagc gccgcataca ccacgacgcc
     2401 gaacccgccg gcgacagcca acaccacgat ggctgcacga acgacgacga ccgacacccc
     2461 ccagcaggcc gcggtctgtc cacagatacc ccagatcacg gcgtcgctgt gggcgggggt
     2521 gtcggcgagc gggttcggtt cgaacacgga catgattggc catgattgca gccgaccgcc
     2581 gactccacat cggggacgaa ccctgagagg atctcagggt tccctcaggg tcgaaccagc
     2641 atttccccga taccgctacg cgccgcgatc atcgacgatg actccatgac caccactcct
     2701 ccaccgactc ctccggacat ctcggcgagt ccagccaccg atccgccgcc cgacctgccg
     2761 cccgacctgc cgcccgacct gccacccgag cagccgcccg agcagccgac ggggtcagca
     2821 accgacggcc cctccgacgg cccctccgac agctctcccg atagccccgc cgacagcctg
     2881 accggcggcg atcggtccga ccttcgccgt tggtgggact cgctgtaccg catcgatacc
     2941 aaccgcatcc tcggcggcgt gtgcggcggg ctgggccacc gcttcggcat gcctcggtgg
     3001 ctcgtgatcc tcggcctcgt gctgatcatc atgctcggcc cgggcgtgcc cctctatctc
     3061 ggcctctggc tgttgctgcc cgacgagtac ggccggcgga tactccagga ctcccgctgg
     3121 cgggacctgg cgatcgccgc cctcggcgtg gtcgtcgcgt tcaccgcgct cggactgggc
     3181 ggcaatctgt tcttcctcaa ggaactcggg acggtcgctc cgtgggcggt catcctcgtc
     3241 ggtatcggac tgctgacccg acggcccggg gcgccgaccg cgtctcctcc tcagtcaacc
     3301 tcggcacacc tgtccgaggc gcaggccgtc tccaccacga tggcttcgac ggcgacagcg
     3361 gcagcgacac cacccaaggc gacaacactc cgcggatctg gcgaaccccg gaagcgccgg
     3421 acctaccgca cccccgtcat cggcccgttc acgtggtgtg tcgcgctcat cgccgcggcg
     3481 ctactcggac tcgtcgcact cggcgatcca cgttcgatcg gcccgggggt gatcgtcgct
     3541 gccgtcacga tcgtgttcgg cgcggggctc gtcttcagtg catttcgcgg gcgagcacgt
     3601 ggtctcgtcc ttccggcctt ggcgggaatc tgtctactcc tcggactcgg agtgatcgac
     3661 ctgcgcgccg acacgttcct gagcgcggtc gacgtgcgct atgtaaccca ggaggagtta
     3721 ccgcggcgcg tcgaatccgg atggggccgc acgcgggtgg acctgtccac actcgaactg
     3781 cagtcggaca cgtcgatcga catcgaagct cccgccggaa tcacgaccgt cgtcctcccg
     3841 gccgccgcga actccgaggt ccacgtcgat atcggtgccg gcgatgctcg tcgttacggc
     3901 gcgttgccca tgaacgatga cctgcgacgg tgggccacct cgagcccgct tccctcgacg
     3961 ggggatccga tcgataccga tcgagcgagc cgcctgtccg atcagtatgc ggttgccgag
     4021 gtcgcgatcg cgaccagaga ctcgacgatg tcggtcgaca ccggctcgga tcacaccctc
     4081 gtcgtcaaca tctcgctcgg ggccggcgag gtccgcgtca tcgaaccggt gtgggccagc
     4141 gtgccgacgt cggtgatgac cccgcagcgg gcgtgtctcg ctgcggtcgg ggccgacgct
     4201 gagatcggcg ccaccgcgga aggcggcgcc accgcggaag gcggcgccac ctcggaagcg
     4261 gtcaccgacg atgagatggt cgccgtgccg tgcacctcag tcgaggaccc ggcgtcgctg
     4321 gtgcccgcct gctatgaccg cttcactggg tggctcgcgg actgtgcagg gcctcgagac
     4381 caatggaccg acgtggtgcc cgcgtgtttc aactgggaga ccaacatcgt gacctgtgaa
     4441 gaggtcggcg tgtcgacagg gctcggcttc tcttccggtg ccacaccgcc gaccgacgcg
     4501 gttccacaga ccgaggtgga ccccaatccg gcgtccgtgc cggacacttc tctcgatgcc
     4561 acaggcggca cccaaccgga aggaacgaac tgatgaaacg tcatcccctc gacgcgatcg
     4621 cgctgatcgc cggcctcctc acgatcggct tcggcctgtt gggactcgcc caccaactcg
     4681 gtgcgtttcc actcggcccg ggcggtgtcg cactggccat ctgtctcgct gtcgggttcg
     4741 gcggaatcgg actgctggtg ctcgctccca agacccgccc cgacgtctcg gatccccgcc
     4801 tttccaaacg cggccgctga cgccaccggc ggcccgacga ccgatcggta caatcgctgc
     4861 catggccgat tcctcaacgt tgcccgcccg agtcgagttc accgtgaccg acgaggggcc
     4921 gggcaccgag acgatgacgc tggtgggcga cgcccacggc aatccgtccg gacagccggt
     4981 ggtgctgttg cacggtgggg gtcagacccg acactcgtgg ggcaacaccg cggcgagcat
     5041 cgctcacgac ggctggcgcg cgtacgccgt ggacatgcgc ggccacggcg agagcggcta
     5101 ctcccccagc ggcgcgtaca ccgcctcgcg attcggccac gacctcgtcg ccatcgcgtc
     5161 acacctcgac tcgaagccgg tcgtcgtcgg agcgagtctg ggcggcctcg ccgggttgct
     5221 cgccgcaggc tcctactccc ccgactcctt cgccgcgctc gtgctcgtcg acatcacgcc
     5281 ccgcccggag accgtggggg tgaatcgtgt tgtcggattc atgttggacc gggcggagga
     5341 aggattcgcg accctcgatg aggcggccga tgccgttggg cgctacaacc ctcaccgccc
     5401 ccggccgacc gacctgtcgg gcctgcgaaa gaatcttcgc ctcggcgagg acggccggtg
     5461 gcgctggcac tgggacccgg cgttgttcaa tccccagaat ggtctcgccg gggttcagga
     5521 gccggggcag ttccagcgcg cggcggaggc actggcgatt ccgacgctgc tcgttcgggg
     5581 ccgacagagc gacctggtca gccccgaatc cgcacaggag ttcctggcga cggttccgca
     5641 cgccgaattc gtcgatgtgt caggtgccgg gcacatggtc gccggcgacc gcaacgaccg
     5701 cttctgcgac gccgtcagcg agttcctgtc acgccgctga cccaccgctg gcgcgctgct
     5761 ggcggcggcc ggggctcggg ctaggtgaaa ttacccacgt gtgaggcgaa cgcgtgttct
     5821 ggttcctgat actctgaaca catgttcgat cttctcacct cacctga
//
LOCUS       DAOOMA010000084         5827 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_16445_length_5827_cov_3.879764, whole genome shotgun sequence.
ACCESSION   DAOOMA010000084 DAOOMA010000000
VERSION     DAOOMA010000084.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 5827)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5827)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5827
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16445_length_5827_cov_3.879764"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..618
                     /locus_tag="PLP95_03650"
     CDS             <1..618
                     /locus_tag="PLP95_03650"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HPB44925.1"
                     /translation="PTTLRRIDPTLFERILLGGSAIPPDRPANTVATYGMTESGAGVV
                     YDGLALNGIGMQIALDGEILLRGPSMLRCYRDGTVPFDEGGWYHTGDLGTIDADTGRL
                     SVAGRADDMIITGGENVWPHLVEAVLLGHPSVEDVAVVGRDDPEWGQRVTAVVVAAAD
                     APAVRLAELREFVKADLPSWCAPKALEFVDRLPKTALGKLQRKLL"
     gene            complement(702..2246)
                     /locus_tag="PLP95_03655"
     CDS             complement(702..2246)
                     /locus_tag="PLP95_03655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB44926.1"
                     /translation="MLVGADVEELERMATRLEQRGVALRRIRREVDAALNSATWHGER
                     ANAFRRSWTQRHGAAIDSTATLAVGTARIVRHQAAQQRHTSATGAEAPPRSVALQRGV
                     AAVADGAVSGVSAALAASVSATGLVPAPATGRSPATGAPADVRPQVAAEPMPTETGSL
                     SLELSGGVLAAAHVGSTVDYVRNPDGTYTVTVSGAIGAGVDSSLRIGSRNVVDLGIEA
                     KVSGIERSTYIVEGRDLNGVLMAFGAQQVSPEFAKMLSMASSSQRWFFDAIDRLTPDW
                     LGSAAATGAKAGVLAPIPAAVSIETGLALDGSVVAKLSETVLPEPIARLLEVLPTASA
                     DVALFHDEVGGATTIEFGGALAGLVGTALGGVVGSGSSLGDSLVSGSKVGASANAAVG
                     SVNGSVSLELNRHLRPVGATITTEATRGSQVEVTRRQVTFDEASAGSAMVSLVEASAR
                     GDVAGAAGAGRRVVEGSSVSEGRAMYQTASTDAGVEVFGTGFGIDIDRRSILRTSSSS
                     SRGGSW"
     gene            complement(2358..4109)
                     /locus_tag="PLP95_03660"
     CDS             complement(2358..4109)
                     /locus_tag="PLP95_03660"
                     /inference="COORDINATES: protein motif:HMM:NF020976.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase D family protein"
                     /protein_id="HPB44927.1"
                     /translation="MADNDPISAHRIDRRSFLSGVGAAAVTVAVASCSSGDGASTGGS
                     GGGSGGASTSTSESPEVAAVPAPSAIGLPAGVFSLGVASGDPTDTSVMLWTRLVADPL
                     AAATAMGERDLEVAYDVALDEGFERLVASGLASAPARLAHSVHLDVTGLDPGSWYHYR
                     FRIGDQTSSTGRTRTMPAPGAAGVTPGGAAAEQFRFVVASCQDYQWGHYAAWRRAVET
                     PDLDAVLFLGDYIYEYSVGDRSPGETGERVWANEETHSLEQYRVRYGQVRSDPALIAA
                     HEAVPWQITFDDHEVSNNYAGDVGQDDVDQPLSRDRRLAGYQAWYEHMPVRLDGVPES
                     FESLRVHQALRFGDLVTLYAIETRQHADPAPCRVEMPEGTPPLLYSDDRPSCEQRFDP
                     ERTNLGAAQEAWLLDELAASDTTWNVLANPIMFSGMNLATREAPEYTLDTWDGYVASR
                     ARVLGALAEVSNPVIVTGDWHASFVLDVRPELIAEADDPAAVLPDGPPSSPPAMTEFV
                     FTAISSVIFAQDYRAANDHIRYFEARNGYGVVTVTPEQLGCEFVYVDDVWDPDSPATT
                     VDRWAVDAGSPQARRVG"
     gene            complement(4131..4871)
                     /locus_tag="PLP95_03665"
     CDS             complement(4131..4871)
                     /locus_tag="PLP95_03665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HPB44928.1"
                     /translation="MELRLDGKVALVTGASKGIGRAIAATFAAAGAQVMISSRKIDAL
                     EAAAAQMPGEVAVFAANAGDLDAASACVDATVERFGGIDILVNNAATNPHMGPTLSIE
                     APQFDKIVEVNLRGPLFWTKAAWNASMKDRPGGSVINISSIGGLSVESSICNYNVSKA
                     GLIHLTRTLANELAPTVRVNAIAPGLVKTDMARALWEQGEEAIASMIPARRLGETDDI
                     ANAALFLASDAASWVCGHTLVVDGGMLL"
     gene            4961..>5827
                     /locus_tag="PLP95_03670"
     CDS             4961..>5827
                     /locus_tag="PLP95_03670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB44929.1"
                     /translation="MKHKLFALLGGTIALVVVVILALSILAQRTQMVPSGPPPTLPSG
                     AEQFSVIRAFADEVAWNRPAAEFGASTRFAQYAERFWNYGSFGGWDDPDKRGALDVRF
                     GDYSMPIYDAREATGTKSVYIANFGYPPYAGYHVEIPWNDHWEPSSGNDAMMIVVNPD
                     TGEAWSTWLLLKSNPSTCLTPSNIAAGFRPFSDLCAGGASRLDNADGSVGDFRTWNSY
                     DSERGAGLPKLAMLTTPYEVRAGAIDHALALTVFNPMFGPTCTTDTIDTAAAGDSCGF
                     YVSPATRMEWPET"
BASE COUNT          967 a         2134 c         1832 g          894 t
ORIGIN      
        1 ccgacgacgc tacgacgcat cgacccgacc ttgttcgaac gaatcctcct cggcggctcg
       61 gcgatcccac ccgaccggcc cgccaacacc gtggccacct acggcatgac cgagtcgggc
      121 gcaggggtcg tctatgacgg acttgccctc aacggcatcg gcatgcagat cgccctcgat
      181 ggcgagatcc tgcttcgggg gccgtccatg ttgcgttgct atcgcgacgg caccgtcccc
      241 ttcgacgagg gcggctggta tcacaccggc gatctcggca ccatcgatgc tgacaccggc
      301 cgcctcagcg tcgccgggcg agccgacgac atgatcatca ccggcggtga gaacgtgtgg
      361 cctcacctcg tggaggcggt gctcctcggc cacccctcgg tcgaagacgt cgccgtggtc
      421 ggccgtgacg accccgagtg ggggcaacgg gtgaccgccg tggtcgttgc cgcggccgac
      481 gcgcccgccg tgcgcctcgc cgaacttcgc gagttcgtca aggccgacct cccctcctgg
      541 tgtgccccca aagccctcga gttcgtcgac cggctcccca agaccgccct cggcaaactc
      601 caacgcaagc tcctgtagcg acagaccccg cgcaccttgc ccgttctccg ataaccagtg
      661 gtcgtgatca cgaccactgg ttatcggaga acgggcgagg gtcaccagga accgcctctg
      721 gagctgctcg aggacgtgcg caggatcgaa cgacggtcga tgtcgatgcc gaagcccgtg
      781 ccgaagacct cgactccggc gtcggtcgat gcggtctgat acatcgcccg accctcggaa
      841 accgacgagc cttcgacgac tcgacgcccc gccccggccg caccggcaac gtcgccacgc
      901 gcactcgcct cgaccaggct gaccatggcc gatcctgccg acgcctcgtc gaaggtcacc
      961 tgacgcctcg tgacctccac ctgcgaccct cgcgtggcct cggtggtgat cgtcgctccg
     1021 accggccgca ggtgccggtt caactcgagg ctgaccgaac cgttgaccga accgacagcg
     1081 gcattcgccg acgccccgac cttcgaaccg gacaccagcg aatcgcccag cgaggaaccc
     1141 gatccgacca ccccacccaa cgccgttccc accaaccctg cgagcgctcc gccgaactcg
     1201 atcgtcgtcg caccgccgac ctcatcatga aacagcgcca catcggccga tgccgtcggc
     1261 agaacctcca ggagacgggc gatcggctcg ggcaatacgg tctcggacaa tttcgcaacg
     1321 accgaaccgt cgagcgccaa gcctgtttcg atgctcaccg cagccgggat cggtgccagg
     1381 acaccggcct tcgcacccgt tgccgcagca cttcccaacc agtccggggt caagcggtcg
     1441 atcgcgtcga agaaccagcg ctgggaactc gacgccatcg acaacatctt ggcgaactcg
     1501 ggcgacacct gttgggcgcc gaacgccatc aggaccccgt tgagatcgcg tccctccacg
     1561 atgtaggtcg agcgttcgat gcccgaaacc ttcgcctcga tcccgagatc gacgacgttt
     1621 ctgcttccga tgcgcagcga actgtcgacc cctgcgccga tcgcccccga cacggtgacc
     1681 gtgtaggtgc catccggatt gcgcacgtag tcgacggttg agccaacgtg tgccgccgcc
     1741 agcacaccac cgctgagttc gaggctcagc gaaccggtct cggtcggcat cggttcagca
     1801 gcgacctgcg gtcgaacgtc ggccggggca cccgtggccg gcgaccgccc ggtcgccggg
     1861 gccggcacca ggccggttgc ggagaccgac gccgcgagcg ccgccgacac ccccgacacc
     1921 gccccgtcgg cgaccgcggc caccccgcgt tgcagcgcga ccgacctcgg cggagcctcg
     1981 gcacccgtcg cgctggtgtg gcgctgctgg gccgcctggt ggcgcacgat ccgcgccgtt
     2041 ccgacggcca gcgtcgcggt cgagtcgatt gccgcaccgt ggcgttgagt ccacgaacgt
     2101 cgaaaggcat tcgcccgttc gccgtgccac gtcgcgctgt tcagcgcggc gtcgacctcc
     2161 cggcgaatgc gccgaagggc caccccgcgc tgctccaggc gagtcgccat ccgctccagt
     2221 tcctcgacat cggctccgac cagcatcgcc acgctccttt caggtgacga tgagccttca
     2281 cgacctggcc gccgatgtca gtgggggata tcccccgaga cggtctcgcg caaacgacgc
     2341 gggtcacccg acgcgggtca accgacgcga cgagcctgcg gcgaccccgc gtccaccgcc
     2401 catcggtcga ccgtggtcgc cggggagtcc gggtcccaca cgtcgtcgac gtagacgaac
     2461 tcgcaaccca actgctccgg ggtcacggtc acgacgccgt agccgtttcg ggcctcgaag
     2521 taccggatgt ggtcgttcgc cgctcggtag tcctgggcga agatcaccga ggagatggcg
     2581 gtgaacacga actcggtcat cgctggtggc gacgagggtg ggccgtccgg gaggaccgcg
     2641 gccggatcat cggcctcggc gatcaactcc ggccgaacgt cgagcacgaa gctcgcatgc
     2701 cagtcgccgg tgacgatcac cgggttcgac acctcggcga gcgccccgag cacccgcgcc
     2761 cgcgacgcga cgtacccgtc ccaggtgtcg agggtgtact ccggagcctc gcgggtggcg
     2821 aggttcatac cggagaacat gatcgggttc gccaacacgt tccaggtcgt gtcgcttgcg
     2881 gcgagttcgt cgaggagcca cgcctcctgg gcagcaccga ggttggtgcg ctcgggatcg
     2941 aatcgctgct cacaactcgg ccgatcgtcg gagtacagca gcggcggggt cccctcgggc
     3001 atctcgacgc gacacggcgc gggatcggcg tgctgtcggg tctcgatcgc gtagagcgtg
     3061 acgaggtcgc cgaagcgcag tgcctgatgc acccgcagcg attcgaacga ctccggcacc
     3121 ccgtcgaggc gaaccggcat gtgttcgtac caggcctggt agccggcgag acgtcgatcc
     3181 cggctcagcg gctgatccac atcgtcctga cccacatcgc ccgcgtagtt gttggacacc
     3241 tcgtgatcgt cgaaggtgat ctgccacggc acggcctcgt gagcggcgat gagcgccggg
     3301 tcggagcgga cctgcccata gcgcacccgg tactgctcca ggctgtgggt ctcctcgttc
     3361 gcccagaccc gctcgccggt ctcaccaggg gaacggtcgc cgacggagta ctcatagatg
     3421 tagtcgccca ggaacaacac cgcatcgagg tcgggggttt ccaccgcccg acgccacgcg
     3481 gcgtagtgcc cccattggta gtcctggcag gaggcgacga cgaagcggaa ctgctcggcc
     3541 gccgcgcccc caggcgtaac ccccgccgct cccggtgccg gcatcgtgcg ggtgcggccg
     3601 gtcgaactcg tctggtcgcc gatccggaac cggtagtggt accacgaccc cgggtcgagg
     3661 cccgtgacat cgaggtgaac cgaatgcgcg agccgtgccg gcgccgaggc caacccggac
     3721 gcgacgagtc gctcgaagcc ctcgtcgagc gccacgtcgt aggcgacctc aaggtcgcgt
     3781 tcgcccatcg cggtcgccgc cgcgagcggg tcggcgacca ggcgggtcca caacatcacc
     3841 gaggtgtcgg tcgggtcgcc actcgccacc cccaacgaga acacccccgc tggaagcccg
     3901 atagcggagg gcgcaggcac agcggcgact tccggcgatt ccgacgtgct cgtcgacgcg
     3961 ccgccggacc cgccgccgga cccgcccgtg gatgctccgt cgccgctcga acacgatgcg
     4021 accgcgacgg tgaccgcggc ggcgccgacg ccgctcagaa aactgcgacg atcgatccgg
     4081 tgtgccgaga tcgggtcgtt atctgccatg tccccccttt gccgaattca ctacagcagc
     4141 atcccgccgt cgacgaccag cgtgtggcca cacacccacg aggcggcgtc gctggcgagg
     4201 aacagcgcgg cgttcgcgat gtcgtcggtc tcgccgagtc gccgggccgg gatcatcgac
     4261 gcgatggcct cctcgccctg ttcccacaac gcccgggcca tgtcggtctt gaccagaccg
     4321 ggagcgatgg cgttcacccg caccgtcggg gccaactcat tggcgagggt cctggtcagg
     4381 tgaatcagtc ccgccttcga aacgttgtag ttacagatcg acgactccac cgacaacccg
     4441 ccgatcgagg agatgttgat caccgaccct ccgggacggt ccttcatcga ggcgttccac
     4501 gccgccttgg tccaaaacag cgggccccgc aggttcacct ccacgatctt gtcgaactgc
     4561 ggcgcctcga tactcaacgt cgggcccatg tgggggttgg tggcggcgtt gttcaccaga
     4621 atgtcgatgc cgccaaagcg ttctacggtg gcgtcgacgc acgccgatgc ggcatcgaga
     4681 tcgccggcgt tcgccgcgaa cacggcgacc tcgccgggca tctgcgccgc cgccgcttcc
     4741 aacgcgtcga tcttgcgtga ggagatcatc acctgcgctc cggcagcggc gaaggtcgcc
     4801 gcgattgccc gaccgatccc cttcgatgcg cccgtgacga gcgcgacctt tccatcgagg
     4861 cgtagttcca tgggcgcacg ttacccgctc cgcttcggag atagcctcgc cgaatgcacg
     4921 tgcaccaacc ccgccgcgaa cgaccggcgt cccggcaccg atgaagcaca agctgttcgc
     4981 actcctcggc ggcacgatcg cactggtcgt cgtggtgatc ctcgcgttgt cgatcctcgc
     5041 ccaacggacc cagatggtgc ccagcggtcc gccgccgacg cttccttccg gcgccgagca
     5101 gttctcggtc atccgggcct tcgccgacga ggtcgcctgg aaccgccccg cggccgagtt
     5161 cggggcatcg acccgtttcg cgcagtacgc cgagcggttc tggaactacg gcagcttcgg
     5221 cgggtgggac gacccagaca aacgcggagc gctcgacgtc cgcttcggcg actactcgat
     5281 gccgatctac gacgcccgcg aggccaccgg caccaagtcg gtctacatcg ccaactttgg
     5341 ctatccgccc tacgccggct accacgtgga gattccgtgg aacgaccact gggagccatc
     5401 ctccggaaac gacgccatga tgatcgtggt caatcccgac accggcgagg cgtggtcgac
     5461 ctggctgctg ttgaagtcga acccctcgac gtgtctcacc ccgagcaaca tcgccgccgg
     5521 gttcagaccg ttctcggacc tctgcgccgg tggggcgtca cgcctcgaca acgctgatgg
     5581 atcggtcggc gattttcgga cctggaacag ctacgacagc gaacgaggtg ccggcctccc
     5641 gaaactcgcg atgctcacga ccccctacga ggtgcgcgcc ggggccatcg accacgcgct
     5701 cgcgctcacc gtgttcaacc ccatgttcgg cccgacgtgc acgaccgaca ccatcgacac
     5761 cgccgccgcc ggcgactcgt gtgggttcta cgtttccccg gccacccgaa tggaatggcc
     5821 cgagacc
//
LOCUS       DAOOMA010000085         5825 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_16457_length_5825_cov_3.205373, whole genome shotgun sequence.
ACCESSION   DAOOMA010000085 DAOOMA010000000
VERSION     DAOOMA010000085.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 5825)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5825)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5825
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16457_length_5825_cov_3.205373"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1365
                     /locus_tag="PLP95_03675"
     CDS             <1..1365
                     /locus_tag="PLP95_03675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB44930.1"
                     /translation="AVGVALATVAACDTPDQPSIQPTSSIANAATSTPRPATTTPSTK
                     PAPVSPGVQLSVPATTTASLVNNAQRCAQVRAAATANPARFRATSVFNTPAACMERHP
                     ESDRWSSTWFTFANYPGRTDPSRRGELVIAFDAYGIPVYSTADATTTIKVYTAAWSYG
                     HELGGGRSIPWNPKWKAASGKDAKLIILDPASGKEWTLWGVRNPSSLECLSAANLLDG
                     YRLGTDLCTYTAYIGRNVDGSIADATNSDGFSQLGGRGMGRLLGMTLLPTLDEIERGS
                     INHAINMETYATMFGPGCTAEQISAGQAGSKCGYAVAPATKLEWTDTVLKCGSSTMAY
                     TDESRALTVPEGMRFVLNKTDAQIDAWLDAQGYVGQRRSTARIFAVALRDYGWIISGT
                     SCSRSSMAVEGIANPTARERWERLGMTDPTKNDTALLNGLITSANDVVVLRPNHDTLL
                     TAVR"
     gene            1424..1816
                     /locus_tag="PLP95_03680"
     CDS             1424..1816
                     /locus_tag="PLP95_03680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB44931.1"
                     /translation="MFRHSRQTQRPIEDTLRGLPSGFVVYVVEGTYVVVGTTGVFVIA
                     ENSIDLHAAAAAAAIGAHRLRSELADALPFVPFVDAIAVGSCATPSDLPALIVPHDMV
                     ESTIVGGPTVIDWSTLESLQHLSYPVLY"
     gene            1910..2845
                     /locus_tag="PLP95_03685"
     CDS             1910..2845
                     /locus_tag="PLP95_03685"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HPB44932.1"
                     /translation="MPTSTDSAATSSSVSASCAGSAASTDGVTCSYLRLGGRGPRVLL
                     VHATGFCATVWRPFAEALSDRFECWALDVRSHGLSTTAEDGRFEWEATGHDVLAVIEA
                     IDEREGPSPSGWRGVGHSMGAAALLLAEQYRPGTFEAMWLYEPIVFATSLRAQFPEVT
                     DNPLAQGAERRRETFDTRQAALDNFTSKAPMNVFAPGVVEGYLERGFVPVDPADPSGA
                     LTLACDRDIEAQIYRSGHLHHAWEGLGSVHCPVMVAGGPPDSDGPAALASMIAEELPA
                     GRWSRHDELAHFGPFEAPAATAAEAAAFFGEEVPR"
     gene            2842..3672
                     /locus_tag="PLP95_03690"
     CDS             2842..3672
                     /locus_tag="PLP95_03690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HPB44933.1"
                     /translation="MSLPLPSSLSPSKVSAFTDCALAFRFSAIDKVPEAPSAPAVKGN
                     LVHRALELLFLAKPDERTKAFAHACLATAWGEYASDERFLGLGLDEAAAAAFLADAEA
                     MVERYFTLEDPTAFDPIGVELLLSIELDDLLLRGIIDRLDLLPNGDLVVTDYKTGRAP
                     TERQEASKLVGVQFYALLCERLFGKRPAKVQLIYLGANPQIIVATPSEQSTRAVERKV
                     GAIWSAVKRSCESEDFRPKQSPLCSWCSFQQFCPAFGGDPDAAKTVTLDSTLAAPLAP
                     "
     gene            3694..4281
                     /locus_tag="PLP95_03695"
     CDS             3694..4281
                     /locus_tag="PLP95_03695"
                     /inference="COORDINATES: protein motif:HMM:NF013718.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HPB44934.1"
                     /translation="MTNATHPATPGPLGHLLDNRAIEHFDEVVERGAEALRGRGPADR
                     LFYGLSMVGDHGTVWHVIGAGRVALGQESLSTALNRSIALGLEALVLNGPIKWLFRRS
                     RPVVDGDRPLHLRTPRTSSFPSGHSSSGIFSAMLLARTTRVKWPWYVLGAAIGWSRVH
                     VRIHHPSDVVGGYLAGWALGRIAVRILDALDAAER"
     gene            4412..5824
                     /locus_tag="PLP95_03700"
     CDS             4412..5824
                     /locus_tag="PLP95_03700"
                     /inference="COORDINATES: protein motif:HMM:NF014998.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004144 - diacylglycerol O-acyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0045017 - glycerolipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="wax ester/triacylglycerol synthase family
                     O-acyltransferase"
                     /protein_id="HPB44935.1"
                     /translation="MEAPADEHQMSDFEALMWNLEKDPRLSSNMGNLNIFDRCPDVDR
                     LRATLERATVVFPRLRQRVAPVFGRLAPPRWELDPDFDIDHHFKVISLGGRGTRRDLD
                     DYVARSIMTPFDRRRPLWEFVLIEGLRGGKAAMVQRLHHTITDGQGGLRLSLEFLDFE
                     RDAPQRELVEAPVVHHHDPSLVDSVTDAVGHVARRQLGIVRRAASEAASGLRHSATHP
                     TSIADQASRTVEFIGSTMRQVNVGDHPLSSLWVDRSLSRAFRRLDVRLERMRSAANRF
                     EVSINDVFVAGALLGAADYHRSAGTPAERLRMAMPVSHRTDKSVGGNLFAPTQSTLPA
                     TVTTPAEAIAAVHEVLQRTKSESATAAMASFAGAINLLPTSVVTSMGFRIASTVDFVT
                     SNLRAAPFDTFMAGALVESSYPVGPLAGSAFNLTTMSFRGVLDMGLFVDTAAVADVDL
                     LAASLADAYKRIATAKPVLR"
BASE COUNT          955 a         2073 c         1806 g          991 t
ORIGIN      
        1 gccgtcggcg ttgcactggc cacggtcgcc gcgtgtgaca ctccggatca gccatccatt
       61 caaccgacgt cgagcattgc caatgccgcc acttcaacac cgcggccagc gacgacgacg
      121 ccgtcgacga agccggcccc cgtctcgccc ggggtgcagc tcagcgtgcc cgccacgacg
      181 accgcgtcgt tggtcaacaa cgcgcagcgc tgcgctcagg tgcgcgccgc cgcaaccgcc
      241 aacccggcgc ggtttcgcgc cacctcggtg ttcaatacac ccgccgcatg catggagcgg
      301 caccccgagt cggaccgctg gtcatcgacc tggttcacct ttgccaacta ccccggtcgc
      361 accgatccgt cgcgacgcgg cgaactcgtc atcgccttcg acgcctatgg catccccgtg
      421 tactccaccg cggacgccac caccaccatc aaggtgtaca ccgccgcctg gtcctatggc
      481 catgagctcg gcggaggccg ttccattccg tggaatccga agtggaaggc cgcctcgggc
      541 aaggacgcga agctcatcat tctcgacccg gcgtcgggca aggaatggac gttgtggggt
      601 gtacgcaacc cgagcagcct ggaatgcctg tcggcggcga acctgctcga cggctaccgg
      661 ctcggcaccg acctgtgcac ctacaccgca tacatcggcc gcaacgtcga cggctcgatc
      721 gccgacgcga cgaacagcga cgggttctcc caactcggcg gacgggggat gggccggctg
      781 ttgggtatga ccctgctgcc caccctcgac gaaatcgaac gcgggtcgat caaccacgcc
      841 atcaacatgg aaacctacgc caccatgttc ggacccggtt gcaccgcgga acagatctcc
      901 gcgggtcaag cgggatccaa atgcggctat gccgtcgccc cggccacgaa actcgaatgg
      961 accgacactg tcctcaagtg cggttcctcc acgatggcct acaccgacga gtcccgagcg
     1021 ttgaccgtgc ccgaggggat gcgattcgtg ctcaacaaga ccgacgcaca gatcgacgcc
     1081 tggctcgacg cacagggcta tgtcggccag cggcgctcga cggcacggat cttcgcggtg
     1141 gcgttgcgcg actacggctg gatcatctcg ggcacctcgt gcagccggtc ctcgatggcc
     1201 gtggaaggga tcgcaaatcc caccgcccgc gaacgctggg aacgcctcgg catgaccgat
     1261 cccaccaaga acgacacggc cctgctcaac gggctgatca cctccgccaa cgacgtcgtg
     1321 gtgctgcgcc cgaaccacga cacgttgctg accgcggtcc gttgatctgc aaaggatcgt
     1381 caccagcact caaggccgct gtattcgtgc cgatatcggt taggtgtttc gacactcgcg
     1441 ccagacccaa cgaccaatcg aagacacgct tcgcggcctc ccgtcgggct tcgtcgtcta
     1501 cgtggtcgag ggcacctatg tcgtggtcgg caccaccgga gtgttcgtga tcgccgaaaa
     1561 ctcgatcgac ctgcacgccg cagccgcagc cgcagcgatc ggggcccatc gcctgagaag
     1621 cgagttggcc gatgcgttgc ccttcgttcc gttcgtcgac gcgatcgcgg tcgggtcgtg
     1681 cgccacgccg agtgacctgc cggcgctgat cgtgccgcac gacatggtcg aatcgaccat
     1741 cgtcggtggc ccgacggtga tcgactggtc gaccttggaa tcacttcaac acctttcgta
     1801 tcccgtgttg tattgatcgc tcggcctcgg atgccctgac cggcgcgagg gtggtgcagc
     1861 ggcgcccgga tacctcgctg acagtggtcg gtcgtagtgt gaagaagcga tgccgacctc
     1921 caccgattcc gccgccacct cctcctctgt ctccgcctcc tgtgccggct ccgctgcctc
     1981 caccgacggc gtcacctgct cgtatcttcg gctcggcggt cgcgggcctc gcgtcttgtt
     2041 ggttcacgcg accgggttct gcgcaaccgt atggcgcccg ttcgccgaag ccctctccga
     2101 ccgcttcgag tgttgggcgc tcgacgtgcg gtcccacggc ctctccacca ccgccgagga
     2161 cggtcgcttc gagtgggaag ccaccggcca tgacgtgttg gcggtcatcg aggccatcga
     2221 cgagcgcgag gggccaagtc catccggctg gcgtggcgtg ggccactcga tgggggcagc
     2281 ggcgctgttg ctcgccgagc agtaccggcc cggcaccttc gaagccatgt ggctgtacga
     2341 gccgatcgtg tttgcgacgt cgttgcgagc gcagttcccc gaggtcaccg acaacccctt
     2401 ggcccaaggg gccgaacgcc ggcgcgaaac gttcgataca cggcaggcgg cgctcgacaa
     2461 tttcacctcc aaagccccga tgaatgtctt cgctcccggg gtcgtcgagg gatacctcga
     2521 gcgcggattc gttccggtcg accccgccga ccccagcggc gcactcaccc ttgcgtgcga
     2581 ccgcgacatc gaggcccaga tctaccgcag cggacacctc caccacgcct gggaagggct
     2641 cgggtcggtg cactgtccgg tgatggtggc cggcgggccg cccgacagcg acggaccagc
     2701 ggcgctggcg tcgatgatcg ccgaggaact tcccgcgggc agatggtccc gccacgacga
     2761 actcgcccat ttcgggccgt tcgaagcccc cgcggcgacc gccgctgaag ccgccgcgtt
     2821 cttcggcgaa gaagtccctc gatgagcctg ccgctgccct cgtcgttgtc gccctccaag
     2881 gtgtcagcgt tcaccgattg cgcgctcgcg ttccgcttct ccgccatcga caaggtgccc
     2941 gaagccccaa gcgcgcccgc ggtgaagggc aacctcgtgc accgggcact cgagctgttg
     3001 tttctggcca aacccgacga gcgcaccaag gcgttcgcac acgcatgcct cgcgacggcg
     3061 tggggcgagt acgcctccga tgagcgattc ctcggcctgg gcctcgacga ggcggccgcg
     3121 gccgcgttcc tggcagacgc cgaagcgatg gtcgagcggt acttcaccct cgaagacccg
     3181 accgcgttcg acccgatcgg cgtcgaattg ctcctgtcga tcgaactcga cgacctgtta
     3241 ctgcgcggca tcatcgaccg cctcgacctg ttgcccaacg gcgacctcgt cgtcaccgac
     3301 tacaagaccg gcagggcccc gaccgaacgc caggaggcct ccaaactggt cggagtgcag
     3361 ttctatgcgc tgctgtgcga acgcctcttc ggcaaacgac cggccaaggt tcaactcatc
     3421 tacctcggag cgaaccccca gatcatcgtg gcgacgccga gcgagcagtc gactcgcgcg
     3481 gtcgaacgga aggtgggagc gatctggagc gccgtcaaac gctcctgtga gtccgaggac
     3541 tttcgaccga agcaatcccc gttgtgctcc tggtgttcgt ttcagcagtt ctgtccggcc
     3601 ttcggtggcg accccgatgc agcgaagacc gttaccttgg actcgacgtt ggccgccccg
     3661 ttggccccct gacctcgtga acctggtgag acgatgacga acgcaaccca cccggcaacc
     3721 cctggaccgc ttgggcacct gctggataat cgtgcgatcg agcacttcga cgaggtcgtc
     3781 gaacgcggcg ccgaagcgtt gcgcggtcgg gggcccgccg accggctgtt ctacggactc
     3841 tcaatggtcg gcgaccacgg caccgtgtgg cacgtgatcg gcgccggccg ggtcgcgctc
     3901 ggacaagagt cactctcgac cgcgctcaac cggtcgatcg cgctcggtct cgaagcgctc
     3961 gtgttgaacg gaccgatcaa gtggctcttt cgtcgaagcc gaccggtggt cgacggcgac
     4021 cgtccacttc acctgcgaac cccgagaacc tcgagctttc cgagcggcca ctcgtcgtcg
     4081 ggcatcttct cggcgatgct gctcgcccgc acgactcggg tgaagtggcc ctggtacgtc
     4141 ctgggtgccg ccatcgggtg gagccgcgtt cacgttcgga tccatcatcc gtccgacgtc
     4201 gtgggcggct atctcgccgg ctgggcgctc ggtcgaatcg ccgtcaggat cctcgatgcg
     4261 ctcgatgcgg ctgaacgctg atggctgaac gctgaccacc gagccgcctg gcgattgacc
     4321 acccggcggc ctggcgacgc accgataggc accttcccca tcggcggccc tgagcgggac
     4381 gatcgccgga agggccgata gcctgacctg catggaggct cctgccgacg aacaccagat
     4441 gagcgacttc gaggcactca tgtggaacct ggagaaggat ccgcgcctgt cgtcgaacat
     4501 gggcaacctc aacatcttcg atcgctgccc cgatgtcgac cggcttcggg cgacgctcga
     4561 gcgggccacg gtcgtgtttc cccgcctgcg ccaacgggtc gctcccgtat tcgggcgcct
     4621 ggcgccgccg cggtgggaac tcgaccccga cttcgacatc gatcaccact tcaaggtgat
     4681 atcgctcggg ggtcggggga cgcgccgcga cctcgacgac tacgtcgccc ggtcgatcat
     4741 gacccccttc gaccgccgcc gtccgctgtg ggagttcgtg ctgatcgaag gacttcgggg
     4801 cggcaaagcc gcgatggtcc aacggctcca ccacaccatc accgacggcc aaggtggcct
     4861 gcgactgtct ctggagtttc tcgacttcga acgcgatgcc cctcaacgcg aactggtcga
     4921 ggctccggtt gtgcatcacc acgacccctc gttggtcgac agcgtgaccg atgccgtcgg
     4981 ccatgtcgcg cggcgccaac tcggcatcgt ccggagggcg gcgagcgagg ccgcctcggg
     5041 ccttcgccac agcgcaaccc atcccacctc gatcgccgat caggcctccc gaaccgtcga
     5101 gttcatcggg tcgaccatgc gccaggtcaa cgtcggcgat caccctctca gctccttgtg
     5161 ggtcgaccga tcgttgagcc gtgcctttcg gcgcctcgac gttcgccttg aacgcatgcg
     5221 cagcgcggcc aaccgcttcg aggtctccat caacgacgtg ttcgtcgccg gagcgctact
     5281 cggcgctgcc gactaccacc gcagcgccgg gacgcccgcc gagcgactgc ggatggcgat
     5341 gcccgtgagt catcgcaccg acaagtcggt cggcggcaac ctgttcgccc cgacccaatc
     5401 gaccctcccc gcgacggtca cgacaccggc tgaggcgatc gcggcggtgc acgaggtcct
     5461 gcagcgcacg aaatcggagt cggcgaccgc cgcgatggcc tcattcgccg gggccatcaa
     5521 cctcttgccg acctcggtgg tgacgtcgat ggggtttcga atcgcgtcga cggtcgactt
     5581 cgtcacctcg aacctgcggg ccgcgccgtt cgacacgttc atggccggcg ccctggtgga
     5641 gtcgagctac ccggtcggcc cgctcgccgg atcggcgttc aacctcacca cgatgtcttt
     5701 tcggggggtg ctcgacatgg gcttgttcgt cgacaccgca gcggtggccg atgtcgacct
     5761 gctcgctgcg agcctcgccg acgcctacaa gcgcatcgcg accgccaagc ctgttctccg
     5821 ataac
//
LOCUS       DAOOMA010000086         5823 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_16466_length_5823_cov_4.304438, whole genome shotgun sequence.
ACCESSION   DAOOMA010000086 DAOOMA010000000
VERSION     DAOOMA010000086.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 5823)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5823)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5823
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16466_length_5823_cov_4.304438"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..482
                     /locus_tag="PLP95_03705"
     CDS             <1..482
                     /locus_tag="PLP95_03705"
                     /inference="COORDINATES: protein motif:HMM:NF019982.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HPB44936.1"
                     /translation="DAEPSASCTILPTGAATEITTSTAAERVLVIGSVGDPATPYAWM
                     SQMADALGTPKRITYSGTGHGVALSQPCVSAQLVAFLTDPVAFTPTDCEQNPDERDIY
                     ATIAGQFTDLGLPGSTIECIVEGLKERISPLAIAADDGSDIDSELLTEISRIAIGCR"
     gene            611..1447
                     /locus_tag="PLP95_03710"
     CDS             611..1447
                     /locus_tag="PLP95_03710"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF013180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HPB44937.1"
                     /translation="MRRLTAIILGGLGLITGATAMLADASWLGIVAGLAALAAGVVGF
                     SETANNEALQAKAITASDETNRLRAEVERLQVELADERTKRVDAQPPLAEFSTDSAGS
                     FNRKFDDTATPLVNDPRDLSDTKLLADPETNLFSEAYFRVALDARIASARRHLRPVAV
                     AIVEVLEGDDPQFGEEADPQKVTGAIRQTLRDADTACRMTDGRYAIILEDTPENGAVW
                     TVERIRRNLTTRYGHHTMWAGVACYPAHAFSSEELLEQSTAALGAAREWKQDRIEVAT
                     SE"
     gene            1545..4154
                     /locus_tag="PLP95_03715"
     CDS             1545..4154
                     /locus_tag="PLP95_03715"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HPB44938.1"
                     /translation="MAATASATEATHDMDQGRSRVLFVTALVIAALTPMIMWLTQFQG
                     PDSALIILLVTGLSAVFNNVNIDFEFRHENHRFMFLAIPMLVGLSFLSPLTLLAIQLA
                     VAGLTYRVVENQPLMKLVVNLAIVGLDTVVAATVFWLWIGPENSGVLSWFVLLGGFVL
                     AEAAGGFVTIAVMSSFLGRLITPSGTDLVLTLIGIVADVTLAVVFVTLLRLNSAAAAL
                     VLVIGVTMYLLAREYTRISTRYRGMNRLNNFAIGISSAVNDGTVFEELLYQAAETLHA
                     ETAWITLERFGEVTRFEIRDRQLVQTPSTQLDRLLLDLAPIEPQIFTVEDETTPSAFA
                     KALNDSGHEEIVAVELTRQDSTRLVLVIADRSTEVRPFDREDADMLGTVGVHANLSLQ
                     NVDLVERLRLQVQESDYLASHDSLTDLPNRSLLLHTLDEMLGRGDDVAVILLDLDRFK
                     EVNDTLGHENGDLVLIEVAARLSSLLEDGEMAARLGGDEFAVIVRRGSRCDTGALTDD
                     AFVAARNIANNLHRSFAIGDVAIDIAASVGISLPVDPRGRDGGRRLDAGTMLRQADVA
                     MYLAKEGRTVIECYSSERDHYNPERLAMASRLRAAIDHGELVLHYQPQVHIRSGVVES
                     VEALVRWPQPDGSFVPPDSFISLAEHTGLIHQLTRFVVSSAVEQAAIWYAAGTPRRVS
                     MNLSAYNLTNESIVDLYSELLAHHRLPPELLVAELTESAVISDSRRAVAVMERIRQLG
                     IGISIDDFGTGQSSLSYLANLPATELKIDRSFITPLGTEPTAEIIVRATVELAQSLRM
                     HVVAEGIETEEQLAMLSDIGCDYAQGFYFSRALEPRGLELWVEQHRADAAQPRLETRE
                     VATNNGAPTSSFR"
     gene            complement(4183..>5823)
                     /locus_tag="PLP95_03720"
     CDS             complement(4183..>5823)
                     /locus_tag="PLP95_03720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HPB44939.1"
                     /translation="DAGNMLKPMLARGELRMVGATTLDEFRKYIEKDPALERRFQQVY
                     VGEPTVEDTIAILRGLKERYEVHHGVRIADSALVAAAMLSDRYLTGRFLPDKAIDLME
                     EAASSLRIEIDSVPTEIDVVERRIRGLDMERVALSKETDAASTERLLAIDKELADLRE
                     QADAMRAHWQSEKDAIAVIQELKERQEHLKAEADRCEREGDLSRASELRYGHLPILDR
                     EVEAASEQLAALQTDKQMLKEEVDESDIAKVVGRWTGIPVSRLVEGEMGKLLRLEEVL
                     HERVVGQDEAVEVVSNAIRRSRAGLSDPHRPIGSFLFLGPTGVGKTELARTLADFLFD
                     DERAMIRIDMSEYMDKHTVSRLIGAPPGYVGYDEGGQLTEAVRRRPYAVVLLDEVEKA
                     HPDVFNVLLQLLDDGRLTDGQGRTVDFSNVILIMTSNLPGDPIDFFRPEFINRLDDIV
                     RFRPLAQSDLTHIVDIQLAHLQQRLADRRITLTVSDAAKQRLSEQGYDPQFGARPLKR
                     LIQREIGDRLAVALLDGTVTENSTVVVDLVDDEYAITA"
BASE COUNT         1048 a         1984 c         1750 g         1041 t
ORIGIN      
        1 acgacgctga gccgtcagcc agctgcacga tcctgccgac cggtgcggcc accgagatca
       61 ccacgtcgac ggcggccgaa cgcgtcctgg tcatcgggtc cgtcggcgat ccggcgacac
      121 cctacgcttg gatgtcccag atggccgacg cgctcggcac gccgaaacgc atcacctaca
      181 gcgggaccgg acatggtgtt gctctgtcgc agccgtgtgt gtccgcccaa ctcgtagcgt
      241 tcctcaccga tccggttgcg ttcaccccca ccgactgcga gcagaaccct gacgaacgcg
      301 acatctacgc cacgatcgcg ggacaattca ccgatctcgg gcttccgggc tctacgatcg
      361 agtgcatcgt cgagggcctg aaagagcgca tctcaccgct tgcgatcgcc gccgacgacg
      421 gcagcgacat cgactcggaa ctcctcaccg agatctcacg gatcgctatc ggatgccgat
      481 gagaagtgag gaaattaatt attgagttcc gacacagacg cgtcgagaca ttctctgacc
      541 gacgacaccg aagccaccat ggcgccgatg tccggtcacc tgctgaaacg gtaactggag
      601 cgacaatcct gtgcgacgac tgactgcaat catcttgggc ggcctcggcc tgatcaccgg
      661 cgccaccgcc atgctggccg acgcgtcctg gctgggcatc gtcgccgggc tcgctgcgct
      721 cgccgccggt gtcgtcggct tcagcgagac cgccaacaac gaggccctcc aggccaaggc
      781 gatcaccgcg tcggatgaga cgaaccggct tcgggccgag gtcgagcggc tgcaggtgga
      841 actcgccgac gaacgcacca aacgcgttga tgcacaacca cccctcgcgg agttctccac
      901 cgacagcgcc ggttcgttca accgcaagtt cgacgacacc gcgacgccgc ttgtgaacga
      961 tccgcgagac ctgtcggaca cgaagttgct cgccgacccc gagaccaacc tgttcagtga
     1021 ggcctacttc cgcgtcgcgc tcgatgctcg gattgcgtcc gctcgtcgtc acctgcgtcc
     1081 cgtggccgtg gcgatcgtcg aagtactcga aggcgacgat ccgcagttcg gggaggaagc
     1141 cgatccccag aaggtgaccg gcgctatccg tcagaccctg cgggacgccg acaccgcgtg
     1201 ccggatgacc gacggccgct acgcgatcat cctcgaggac accccggaaa acggagcggt
     1261 gtggaccgtc gagcgtattc gtcgaaacct caccacccgc tacggccacc acacgatgtg
     1321 ggccggggtg gcgtgctatc cggcccatgc cttctcctcg gaggaactgc ttgagcagtc
     1381 cacggccgcc ctcggggccg cccgagagtg gaagcaggac cgcatcgagg tcgcgacctc
     1441 cgagtagggc tcccggctca cgggcaacct cacctcttcg ggcgggtgga aaattgctca
     1501 cctcataggc cagcgtgccg atagaaggca tgaggttttg tgctatggcc gcaactgcat
     1561 cggcaactga agcgacccac gacatggacc agggacgttc ccgggttctg ttcgtgaccg
     1621 cgctcgtcat cgccgcgttg acgccgatga tcatgtggct cacccaattt caggggccgg
     1681 atagcgcact gatcatcctg ctggtgaccg ggctgtcggc ggtgttcaac aacgtgaaca
     1741 tcgacttcga gttccgccac gagaaccacc ggttcatgtt tcttgccatc ccgatgctcg
     1801 tcgggttgag ttttctctcg ccactgaccc tgttagccat ccaactcgcg gtcgccgggt
     1861 tgacctatcg cgtcgtcgag aatcaaccgc tgatgaagct ggtggtgaac ctcgcgatcg
     1921 tcggcctcga caccgtcgtc gccgccaccg tgttctggct ttggatcggg ccagagaaca
     1981 gcggcgtact ctcgtggttc gtgctcctgg gcggattcgt gctggcggag gcggccgggg
     2041 gattcgtcac gatcgcggtc atgagcagct ttctcggcag gctcatcacc ccgagcggaa
     2101 ccgacctcgt tctcaccctc atcggcatcg tcgccgacgt cacgctggca gtggtgttcg
     2161 tgacactgct gcgcctcaac agcgctgctg cggcactggt gctcgtcatc ggggtcacga
     2221 tgtacctgct ggctcgcgaa tacacccgca tcagcacccg ctatcgggga atgaatcggc
     2281 tgaacaactt cgcgatcggc atctcctcgg cggtcaacga cggtaccgtt ttcgaggaat
     2341 tgctgtatca agccgccgag acccttcacg ccgagacggc gtggatcacc ctcgagcggt
     2401 tcggtgaggt gacccggttc gagattcgcg accggcagct tgtgcagaca ccgtcaacgc
     2461 aactcgatcg gcttctgctc gacctcgctc cgattgagcc ccagatcttc accgtcgagg
     2521 acgaaacgac tccgagcgcg ttcgcgaaag ccctgaacga ctccggtcac gaggagatcg
     2581 tggccgtcga gttgacccgc caagattcga ctcgcctggt gctcgtcatc gccgaccgca
     2641 gcaccgaggt tcggccattc gatcgagaag atgccgacat gttgggcacc gttggtgtgc
     2701 acgccaacct gtcgctgcag aacgtcgatc tcgtcgagag actccggctg caggtgcagg
     2761 agtccgacta cctcgcctcc cacgattcgc tcaccgacct tccgaaccga tcgctgctac
     2821 tacacaccct cgacgagatg ctcggccgtg gcgacgacgt cgccgtgatc ctgttggacc
     2881 tcgaccggtt caaggaggtc aacgacaccc tcggccacga gaacggcgac ctggtgctga
     2941 tcgaagtcgc ggcacgtctg agttccctgc tcgaagacgg cgaaatggcg gcgcgcctcg
     3001 gcggcgacga attcgccgtg atcgtgcgac gaggcagccg gtgcgatacc ggcgccctca
     3061 ccgacgatgc gttcgtcgcg gcgcgcaaca tagcgaacaa tctccaccga tcattcgcca
     3121 tcggcgatgt cgccatcgac atcgccgcca gcgtgggcat ctcgctgccg gtggatccgc
     3181 gaggccgcga cgggggacgc cgactcgatg ccggcaccat gctgcgccag gccgacgtcg
     3241 cgatgtacct cgccaaagag ggccgaacgg tcatcgagtg ctacagcagc gaacgcgacc
     3301 actacaaccc cgaacgcttg gcgatggcga gccgacttcg cgccgcgatc gatcacggcg
     3361 aactggttct gcactatcag cctcaggttc acattcgttc aggcgtcgtg gagtcggtcg
     3421 aagccctcgt gcgctggccc caacccgatg gctcgttcgt gccgcccgac tcgttcatca
     3481 gcctggccga gcacaccggg ttgattcatc agctgacccg attcgtcgtg tccagcgccg
     3541 tcgaacaggc ggccatctgg tacgccgcag gcactccacg ccgtgtttcg atgaacctca
     3601 gcgcctacaa cctcaccaat gagtcgatcg tcgacctgta ctccgaactc ctcgcccacc
     3661 accggctccc gcccgaactg ctcgtcgccg aactcaccga gtccgccgtc atctccgatt
     3721 cacgccgggc ggtcgcggtg atggagcgca tccgacagct cggcatcggc atatccatcg
     3781 acgacttcgg aaccgggcag tcatcgctgt cctacctcgc caaccttccg gcgacagaac
     3841 tcaagatcga tcggagcttc atcacaccgc tcggaacgga gccgaccgcc gagatcatcg
     3901 tgcgcgccac cgtggaactc gcccagagcc tccgcatgca cgtcgtcgcc gaaggcatcg
     3961 agaccgagga acagctggcg atgctgagcg acatcgggtg tgactacgcc cagggcttct
     4021 acttctcgcg tgcgctcgag cctcgcgggc tggagctgtg ggttgaacaa caccgcgccg
     4081 acgctgctca accccgcctc gaaacgcggg aggtcgcgac gaacaatggc gccccgacct
     4141 cctcgtttcg ctgacgatct cgacctcgac cggccgcctc gctcaggccg tgatggcgta
     4201 ctcgtcgtcg acgaggtcga ccaccacggt tgagttctcc gtcacggtgc cgtcgagcaa
     4261 cgcaacggcg agccggtcac cgatctcacg ctggatgagc cgcttgagcg gacgggcgcc
     4321 gaactgcggg tcgtagccct gctcggacaa ccgctgcttc gcggcatcgc tcacggtgag
     4381 cgtgatgcga cgatcggcga gacgctgctg gagatgggcc agctgaatat cgacgatgtg
     4441 ggtcagatcc gattgcgcaa ggggacggaa ccgaacgatg tcgtcgagac ggttgatgaa
     4501 ctccggccgg aagaagtcga tcggatctcc cgggagattc gacgtcatga tcaagatcac
     4561 gttcgagaag tcgaccgtgc gaccctgccc atcggtcaga cgcccgtcgt cgagcagctg
     4621 cagcagcacg ttgaacacgt ccgggtgcgc cttttcgacc tcatcgagca gcaccacggc
     4681 atagggccga cgccgcacgg cttcggtcag ctggccgccc tcgtcgtagc cgacgtagcc
     4741 gggaggggcc ccgatgagcc gagacacggt gtgtttgtcc atgtactcgc tcatgtcgat
     4801 gcggatcatc gcccgctcgt catcgaacag gaaatcagcg agcgttcgag cgagttcggt
     4861 cttgcccaca ccggtcgggc cgaggaacag gaacgaaccg atgggacggt ggggatccga
     4921 cagacccgca cggcttcgcc ggatcgcgtt cgacacgacc tcgaccgcct cgtcctggcc
     4981 gacgacccgt tcgtgcagca cctcttcgag ccgcaacaat ttgcccatct ctccttcgac
     5041 aagtcgcgac accgggatgc cggtccagcg tccgaccacc ttggcgatgt cgctttcgtc
     5101 gacctcctct ttcagcatct gcttgtcggt ctgaagtgcc gcgagctgct ccgacgccgc
     5161 ctccacctcg cgatcgagga tcggcagatg cccgtaacgc agctccgagg cgcggctcag
     5221 gtcgccctcg cgttcgcagc ggtcggcctc tgccttgagg tgttcctgac gttccttcag
     5281 ttcctggatc acggcgatgg cgtccttttc cgactgccag tgcgcccgca tcgcgtcggc
     5341 ctgctcgcgc aggtcagcga gttccttgtc gatcgccaac aaccgctcgg tcgaggcagc
     5401 atcggtctcc ttcgacagcg cgacgcgctc catgtcgagg ccgcgaatcc gtcgctcgac
     5461 cacgtcgatt tcggtcggaa ccgaatcgat ctcgatacgc agcgagctcg ccgcctcctc
     5521 catcaggtca atcgccttgt ccggcaggaa ccggccggtg aggtagcgat ccgacaacat
     5581 ggcggccgcc acgagcgccg aatcggcgat ccgcacaccg tgatgcacct cgtagcgctc
     5641 cttgaggccg cgcaggatgg cgatggtgtc ttccaccgtc ggttcgccca cgtacacctg
     5701 ttggaaacga cgctcgagcg ccggatcttt ctcgatgtac ttgcggaact cgtcgagcgt
     5761 ggtggcaccc accatgcgaa gctcgccgcg ggccagcatc ggcttcagca tgttgccggc
     5821 gtc
//
LOCUS       DAOOMA010000087         5821 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_16482_length_5821_cov_4.201353, whole genome shotgun sequence.
ACCESSION   DAOOMA010000087 DAOOMA010000000
VERSION     DAOOMA010000087.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 5821)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5821)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5821
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16482_length_5821_cov_4.201353"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..357
                     /locus_tag="PLP95_03725"
     CDS             <1..357
                     /locus_tag="PLP95_03725"
                     /inference="COORDINATES: protein motif:HMM:NF018028.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HPB44940.1"
                     /translation="TLGKRMCRLVVVTTSDAPIGWIRSFARFAVAFGAFALGPWIARL
                     ETGLLHDVLAVVQVAVPLIVYLPILLRPDRRGLHDLAAGTVVRSLVPPLSSLLSNPGG
                     PDGLGRQPNRASSIDQ"
     gene            complement(324..701)
                     /locus_tag="PLP95_03730"
     CDS             complement(324..701)
                     /locus_tag="PLP95_03730"
                     /inference="COORDINATES: protein motif:HMM:NF024092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HPB44941.1"
                     /translation="MDTAAMKQAVEAYTRLHSARDLDALVDLFAEDAVAYDPVDADPH
                     VGKAAIREFFSGTHQMADEFKLELTGPVRAVADFAAFPMTVTTKIGEMSMQLDIIDVF
                     TFNDAGKVVEMKAYWSMDDARFG"
     gene            824..1852
                     /locus_tag="PLP95_03735"
     CDS             824..1852
                     /locus_tag="PLP95_03735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB44942.1"
                     /translation="MSDKTKLTELVTALAMLGGDHPLAVLPVQPAELRNVDDATWTEL
                     VQSWNEPDLRPLLDAAFDNGRFFREATDALRGRHPRIIEWTGPQRSPGDEVAPIDLRV
                     DHVYLVSCKYLSKITMNASPAHLFDRLLTGGHGRRSTDWFCEVASREREILWRHCRQW
                     LHETFDDFEAPHDPGDLDRTGRRELARRLRVGSKAWPPELVEPYRELCVAVSQRSAQR
                     WSESLAKQRGAAEAMLWRLLRIGSAPYFVLGSDLSAAAPPLRVRVASPWDWRQRFQLR
                     DFSVAPLDGGQPMVTWRARYRDEHGDHEVRGHVEIRWSHGRFNGPPEAKVYLDTPFDL
                     VPGYFPIQ"
     gene            1884..2279
                     /locus_tag="PLP95_03740"
     CDS             1884..2279
                     /locus_tag="PLP95_03740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03618 family F420-dependent PPOX class
                     oxidoreductase"
                     /protein_id="HPB44943.1"
                     /translation="MTIDPRNPGPEALAFLAEYHLGVLGTHRSDHSIHLVPVGFTYDP
                     ETQLVRIITRSGSRKVRNIAARPGHRVSVSSVDGGRWLTFEGPAEVSSAPDRVAEAVE
                     RYAARYRQPGESDDRVVIEISVDRILGRA"
     gene            complement(2330..3379)
                     /locus_tag="PLP95_03745"
     CDS             complement(2330..3379)
                     /locus_tag="PLP95_03745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB44944.1"
                     /translation="MPKGQGRSGRVLGMLVAASALLAVACSDGSSSTLERSRTQPTTT
                     VIERSAPTTTVGAQTPTTRTKVDELPGLWIATEHNVQDDAGVVVAEAQDDSEVFRSPL
                     DDGAGGVIYLRCTDGSERACAIERVAAGTEDAVVLGSALRLFALGTWNERPVVVTGFV
                     DPARTADFETDRSDLLVGLVDLETGELVAAADWYGWESGPFAIDVEAAMVAVCIGEGE
                     TCELGTATDPAQMPLALEGAADGATVTSLALSADATVLTWVESEPMGGTVVAHHRDLL
                     GTATESIELRSSDASSPQEAITDGYWAAVRVDEVVYFTELLGGVGTGSKMVPIDTREV
                     ALRAGGGASGAQSKL"
     gene            complement(3461..>5821)
                     /locus_tag="PLP95_03750"
     CDS             complement(3461..>5821)
                     /locus_tag="PLP95_03750"
                     /inference="COORDINATES: protein motif:HMM:NF017984.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0036297 - interstrand cross-link repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crosslink repair DNA glycosylase YcaQ family
                     protein"
                     /protein_id="HPB44945.1"
                     /translation="PEAEEVLDPADLMLDPDEVTEVVVSMLPTTAMFASRFREAAARA
                     LLLPRPRVDKRTPLWQQRQKAATLLSVASKYPTFPILLETTRECINDEFDLPSLVDLM
                     TKIRSQEIRVVSVDTDVASPFAASLLYSWIAVYMYEGDAPLAERRAAALGLDKNLLRE
                     LLGSEELRSLLDAEVVAAVEDELQRRAQGRRARDLDELHDLLRVLGPLEVDEFGPRLE
                     PLSPLASTAALRRAIDELLASRRVIEVAIGHRRVFADAADAGRLRDGLGVALPLGLPA
                     AFTESHDAPLRSLFERYARTHGPFLTHDLVERFALSNETTEAILRAMEEDGSLLRGAF
                     RPDGVTDEWCDPEVLRTIRRRTLASLRREVEAVDPEVLGRFLPDWQRVGRFDRGIDAV
                     ADVVGQLAGVPLVASSLDRDIMATRVGDYRPADLDELCTRGEVVWVGAGSIGGSRQGS
                     DGRVRLVYRTDRALLSAAIASVDPDLAPAGDHHVVLRAALGRRGACFWADLVGEVAAA
                     GLVYDDATVGDALWDLVWNGEVTNDSFAALRARIGPKRSGSSPRRRSRGLRALAATPP
                     SAVGRWSLVTDLVASAPTSTEIAHAVAQQLLDRYGVLTREMALAEGVAAGFAGVYPVL
                     KAMEEQGQIRRGYFVAGLGAAQFASHAAVDRLRSMRSEPDDAMSAPAAVLLAATDPAQ
                     PYGAVLAWPDNDGRPTRSAGARVVLCAGEPIAWLDRNGRRVVTFPAASSSSVWVDTVV
                     DALRIGRLDKLEIVDIDGRPASAGPWAEVLRTAGFSEGYRGMVLRR"
BASE COUNT          987 a         2076 c         1863 g          895 t
ORIGIN      
        1 acgttgggca agcggatgtg ccggctcgtc gtggtcacca cctccgacgc accgatcgga
       61 tggatccgga gtttcgcccg cttcgccgtg gccttcggtg catttgcact cggaccctgg
      121 atcgcccggc tcgagaccgg ccttctgcac gacgtactcg ccgtcgtgca ggtcgcggtg
      181 ccgctgatcg tgtatctgcc gattctcctg cgacccgacc gtcgcggcct gcacgacctc
      241 gcagccggaa ccgtcgtgcg ctcgctggtg ccaccgttgt cgagcctgtt gtcgaacccg
      301 ggaggccccg acgggctggg tcgtcagccg aaccgggcgt cgtccatcga ccaataggcc
      361 ttcatctcga cgaccttgcc ggcatcgttg aacgtgaaca cgtcgatgat gtcgagctgc
      421 atcgacatct cgccgatctt cgtggtcacc gtcatcggga acgcggcgaa gtcggccacc
      481 gcacgcaccg gtccggtcag ctcgagcttg aactcgtcgg ccatttggtg cgtcccggag
      541 aagaattcgc gaatcgcggc cttgcccacg tgtgggtcgg cgtcgacggg gtcgtaggcg
      601 accgcatcct cggcgaacag atcgaccagg gcgtcgagat ctcgtgcgga gtgcagccgg
      661 gtgtacgcct cgacggcttg tttcatcgca gcggtgtcca cgtcgtcctc ctcaagcctc
      721 gaccgcggcg gccgatgcga ccgccacgat agcctccgca acccgacgag gccccgacgc
      781 gagggctcag atgcgggtgc gatccccgta ggatcggcac tcgatgagcg acaagaccaa
      841 gctgacggaa ttggtcaccg cgctcgccat gctgggcggc gaccaccccc tcgccgtgct
      901 gccggtgcag ccggccgaac tccgcaacgt cgacgatgca acctggaccg aactcgtgca
      961 atcgtggaac gagcccgatc ttcgtcctct gctcgacgcc gccttcgaca acggacgatt
     1021 cttccgcgag gcgaccgacg cactgcgcgg tcgccatccg cgaatcatcg agtggaccgg
     1081 accacaacga agccccggcg atgaggtggc tccgatcgat ctgcgcgtcg accacgtcta
     1141 tctcgtcagc tgcaagtacc tgtcgaagat cacgatgaat gcgtcaccgg cgcacctgtt
     1201 cgaccgtctc ctgaccggtg gccacggacg gcgcagcacc gactggttct gcgaggtcgc
     1261 gtcgcgtgag cgggagatcc tgtggcgaca ttgtcggcaa tggctgcacg aaacgttcga
     1321 cgatttcgaa gctccgcacg acccagggga cctcgacaga accggccgtc gtgaactcgc
     1381 ccgccgcctg cgggtcgggt ccaaggcatg gccgccggag ttggtcgagc cctatcgcga
     1441 gctgtgcgtc gccgtatcgc agcgcagcgc acagcgctgg agcgagagtc tcgcgaagca
     1501 gcgcggcgcc gccgaggcca tgctgtggcg gctcctgcgc atcggcagcg cgccctactt
     1561 cgtgctcgga agcgacctct cggcggcggc gccgccccta cgagtccgag tggcctcgcc
     1621 gtgggactgg cgacagcggt ttcagctccg cgacttctcg gtcgccccac tcgacggcgg
     1681 ccagccgatg gtgacctggc gtgcccgcta ccgcgacgaa cacggcgacc acgaagtccg
     1741 ggggcacgtt gagatccgct ggagccacgg ccgtttcaac gggccgccgg aggccaaggt
     1801 gtacctcgac acaccgttcg acctggttcc cggctacttt ccgattcagt gaccgccgat
     1861 cgcacccgaa aaggactccg accatgacga tcgatccccg caaccctggc ccggaggcgc
     1921 tggcatttct tgccgagtac caccttggag tcctcggcac tcaccgcagc gaccactcga
     1981 tccacctcgt gcccgtcggg ttcacctacg accccgagac gcaactcgtt cggatcatca
     2041 cccggtccgg ttctcgaaag gtgcgcaaca tcgcggcgag accgggacat cgcgtttcgg
     2101 tttcatcggt cgacggtggc cgatggttga ccttcgaagg ccccgctgag gtgtcgagcg
     2161 cgccggatcg tgtggccgag gcggtcgagc ggtacgcggc gaggtatcgc caacccggcg
     2221 aaagcgacga ccgagtggtg atcgagatat ccgtcgatcg catcctgggc cgggcctgag
     2281 ttcggcctcg gcacgacgaa acggtgtcga ggccacgggg cggccgatat cagagcttgc
     2341 tctgcgctcc ggacgcgccg ccgccagcgc gcagggccac ctctcgggtg tcgatcggca
     2401 ccatcttcga gcccgtcccc actccgccga gcaattcggt gaaatagacc acctcgtcga
     2461 cgcgcaccgc cgcccagtac ccgtcggtga tcgcttcctg aggtgaagac gcgtcactgc
     2521 tgcggagctc gatcgactcg gtcgccgttc cgagcaggtc gcggtgatga gcgaccacgg
     2581 tgccgcccat cggttcggac tccacccacg tgagcacggt cgcgtcggcg gacagcgcca
     2641 acgaggtcac cgtggcgccg tcggccgcgc cctcgagtgc caacggcatc tgggccgggt
     2701 ccgttgcggt gccgagctca caggtctccc cttccccgat acacacggcg accatggcgg
     2761 cctcgacatc gatagcgaat ggaccggact cccagccgta ccagtcggcg gcagcaacga
     2821 gctcgccggt ctccaggtcg acgagaccga cgagcaggtc gcttcggtcg gtttcgaagt
     2881 cagccgtcct ggccgggtcg acgaagcccg tcaccaccac cggtcgttca ttccaggtcc
     2941 cgagcgcgaa gagccgaagc gcgctgccca acacgaccgc atcctcagtc ccggcggcca
     3001 cgcgctcgat cgcacacgcg cgttcggatc cgtcggtgca tcggagatag atcactccgc
     3061 ccgccccatc gtcgagcggc gaccggaaca cctcggagtc atcctgcgcc tcagccacga
     3121 cgacccccgc gtcgtcctgg acattgtgct cggtcgcgat ccagagcccg ggcaactcgt
     3181 cgaccttcgt tcgggtcgtc ggggtctggg ccccgaccgt cgtggtcgga gcgctccgtt
     3241 cgataacggt ggtcgtcggt tgggtccggg agcgctcgag tgtcgacgat gagccgtcgc
     3301 tgcacgccac cgccaacagc gccgacgccg cgaccaacat cccaagaacc cgaccgcttc
     3361 gaccctgacc cttcggcatc gtccgatcat gacagtccgc gggtcttcgc atcgacatgt
     3421 tcctaagccc gctcgcagga cgaacggcga tcagcgtgac tcaccgccga agcaccattc
     3481 cgcggtaccc ctcggaaaac cctgccgtgc gcagcacctc cgcccacggg cctgccgacg
     3541 ccggccgacc gtcgatgtcg acgatttcca gcttgtccaa gcgcccgatg cggagcgcgt
     3601 cgacgacggt gtcgacccag accgaactcg atgaggccgc tgggaaggtc accaccctgc
     3661 ggccattgcg gtcgagccat gcgatcggct cgcccgcaca cagcaccacc cgagcacctg
     3721 cggatcgggt cggtcggccg tcgttgtcgg gccatgccag cacggcgcca taaggctgag
     3781 cggggtcggt cgcggcgagc aacaccgcgg caggcgcgct catcgcgtcg tcgggttcgg
     3841 atcgcatcga acgaagtcga tcgaccgctg cgtgcgatgc gaactgagcg gctcccaggc
     3901 cggcgacgaa atagccacgg cgaatctgtc cctgttcctc catcgccttc agcaccggat
     3961 agaccccggc aaatcccgcc gccactccct cggccaacgc catctcgcgg gtgagcacgc
     4021 catagcggtc gagcagttgt tgcgcgaccg catgggcgat ctcggtcgag gtcggggcgc
     4081 tggccaccag gtcggtcacc agcgaccatc gaccgaccgc gctcggcggg gtcgcggcca
     4141 atgcgcgaag cccacggctt cgtcgccgag gcgacgaacc ggagcgcttg ggcccgatcc
     4201 gagcgcgcaa cgcggcgaac gaatcgttgg tcacctcgcc gttccagacg agatcccaca
     4261 aagcatcgcc cacggtggcg tcgtcgtaca cgagaccggc cgcggccacc tctccgacga
     4321 ggtcggccca gaaacaggcg ccacgccgac ccagcgccgc ccgcaacacg acgtgatggt
     4381 caccggcggg ggcaaggtcg gggtcgaccg aggcgatcgc cgcgctcaac aacgcccgat
     4441 cggtgcgata caccagccga accctgccat cgctaccttg gcgcgacccg ccgatcgaac
     4501 cggctccgac ccacaccacc tcgccgcggg tgcacaactc gtcaaggtct gccggccgat
     4561 agtcacccac ccgcgtcgcc atgatgtcac ggtcgagtga cgacgcgacg agcgggactc
     4621 cggcgagttg gccgacgaca tcggcaactg catcgatccc gcggtcgaat cgtccgacgc
     4681 gctgccagtc gggcagaaac cgcccgagta cctcgggatc gaccgcctcg acctcgcggc
     4741 gcaacgacgc cagggttcgc cgccgaatcg tccgcaacac ctcggggtcg caccactcgt
     4801 cggtgacgcc gtcgggacga aacgcgccgc gtagcaacga cccgtcctcc tccatcgccc
     4861 gcaggatcgc ctcggtggtt tcgttcgaca acgcgaaacg ctcgacgaga tcgtgagtga
     4921 gaaacgggcc atgcgtgcgg gcataccgtt caaacaacga acgcaggggc gcatcgtggg
     4981 actcggtgaa cgctgcgggg agtccgagcg gtagcgccac ccccaaaccg tctcgcagtc
     5041 gaccggcatc ggcggcgtca gcgaacacgc gccggtgccc gatcgcgacc tcgatgacgc
     5101 ggcgcgaggc caacaactcg tcgatcgccc ggcgcagcgc cgccgtcgac gcgagcgggc
     5161 tcaacggttc gaggcgcggg ccgaactcgt cgacctcgag cgggccgagc acccgcaaca
     5221 ggtcgtgcaa ttcatcgagg tcgcgggcac ggcgcccctg cgcgcgacgt tgcagttcgt
     5281 cctcgaccgc ggcgacgacc tcggcgtcga gcagcgagcg caactcctcg ctgccgagca
     5341 gctctcgcaa caggttcttg tcgaggccga gcgcggcggc gcggcgctcg gcgagcggag
     5401 catctccctc gtacatgtac accgcgatcc acgagtacag cagtgacgcg gcgaacggcg
     5461 aggcgacgtc ggtgtcgacc gagacgacgc ggatttcctg cgagcggatc ttggtcatca
     5521 gatcgacgag cgacggcagg tcgaactcgt cgttgatgca ctcgcgggtg gtctccaaca
     5581 ggatcggaaa ggtcgggtac ttcgacgcga cgctcagcaa cgttgccgcc ttctgtcgtt
     5641 gctgccacag cggggttcgc ttatcgaccc gcggccgggg caacagcaac gccctggccg
     5701 ccgcctctcg aaaccgggac gcgaacatcg cggtggtcgg cagcatcgac accacgacct
     5761 cggtcacctc gtcgggatcg agcatcaggt cggccggatc gagcacctct tcggcctcgg
     5821 g
//
LOCUS       DAOOMA010000088         5809 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_16521_length_5809_cov_4.118526, whole genome shotgun sequence.
ACCESSION   DAOOMA010000088 DAOOMA010000000
VERSION     DAOOMA010000088.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 5809)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5809)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5809
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16521_length_5809_cov_4.118526"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(160..504)
                     /locus_tag="PLP95_03755"
     CDS             complement(160..504)
                     /locus_tag="PLP95_03755"
                     /inference="COORDINATES: protein motif:HMM:NF014520.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WhiB family transcriptional regulator"
                     /protein_id="HPB44946.1"
                     /translation="MSALPLTLEPDTSWHLDAACAAATPGTARLFFSDDLSDIIAAKT
                     ICSTCPVISECLDTALARQEQWGVWGGQLIVAGKVVMTKRRRGRPPKTPRPEDQMPQI
                     PFPEHLRHLQPA"
     gene            complement(542..805)
                     /locus_tag="PLP95_03760"
     CDS             complement(542..805)
                     /locus_tag="PLP95_03760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB44947.1"
                     /translation="MRITASSNALDSETRFSERAAWNTPAARSAFHDTFTAATARPLP
                     HPYQHPEGHPMYPTSNPHLATTTSTTSTAGAAKRRHDAQLRST"
     gene            complement(783..995)
                     /locus_tag="PLP95_03765"
     CDS             complement(783..995)
                     /locus_tag="PLP95_03765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB44948.1"
                     /translation="MRTATNNSMTGEATTSSRAHVRVSHLACRAVRHGWQSGTAAIAT
                     PATRIATCAAYATKCNLSALEDHRIE"
     gene            complement(1160..2260)
                     /locus_tag="PLP95_03770"
     CDS             complement(1160..2260)
                     /locus_tag="PLP95_03770"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HPB44949.1"
                     /translation="MTEIERQERLPASEEVTAVAEGIYRLQLPINLPGLGHVNCYALE
                     DERGFTVIDPGFPDPATAAALEQRFAQIGAPTSRIHTVFVTHSHPDHFGGAGRLRYTN
                     EADIVAHRHFATHFDGDDDTELQLADAAPGASFEELRGALEQAVLGDGPIPDLGALRA
                     PWGGSVPMPSRTEIEVVRSWDADTRWGFIHNEPNVRLDHDDVIRLGRREWVAVHTPGH
                     TGDHLCLVDLAEGILFSGDHVLPTITPHISGLRSHTDALAEFFNALDLVRTLPDITLT
                     LPAHGHPFTDLVGRVDAIERHHIDRLERIERIGATLGIATVEAFSRELFAERSWGSMA
                     ESETYAHLEHLVNVGRATRHHRDGFPVYEITV"
     gene            complement(2257..2808)
                     /gene="orn"
                     /locus_tag="PLP95_03775"
     CDS             complement(2257..2808)
                     /gene="orn"
                     /locus_tag="PLP95_03775"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000175 - 3'-5'-exoribonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoribonuclease"
                     /protein_id="HPB44950.1"
                     /translation="MLVWMDLEMTGLDDRTDVIVEIATLITDDDLNIVAEGPDLVIHQ
                     PPEALANMDKVVLNMHTSSGLLDAIRSSEITLEQAGAETLAFIKEHAPQPRSVPLCGN
                     SIGMDRRFLNAYLPQIENHLHYRSVDVSTIKELARRWYPEALAAAPHKATAHRAMDDI
                     RESVAELKYWRDNVFAAATEPNL"
     gene            2872..3309
                     /gene="dut"
                     /locus_tag="PLP95_03780"
     CDS             2872..3309
                     /gene="dut"
                     /locus_tag="PLP95_03780"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HPB44951.1"
                     /translation="MEIPLMRVDPSIEVPEAAKPGDAGVDLRSTIDVVIAPGGHRELV
                     PTGVAIAIPDGYAGFVQPRSGLALKHGVTVLNTPGLIDSGYRGELKVVLVNTDPSEPF
                     HVVRGERIAQLVIQAVERPVFVEVASLDDTDRGTGGFGHTGRG"
     gene            complement(3306..4592)
                     /locus_tag="PLP95_03785"
     CDS             complement(3306..4592)
                     /locus_tag="PLP95_03785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB44952.1"
                     /translation="MALAAEPLDERIESILRWAKAIDRRRAHDQSARIGALATLWALL
                     KFLVLAVGAPLWVPFALFVANTHHRRAKRDIREFPTAVSSISRGRTPSTGADVVVRRP
                     HSERIVIFSDLHRCVPGRLDWPRRQGTKSLYAEVLRRYADDGWTLCENGDVEDFWMVG
                     GSTLGATYDALRLLGGALGDDRLSTALYRQYLAAIIDNNDEIYSVIASRFAADGRYLR
                     TVGNHDDPLHHHEVARVLTDRLGPFPLADFITLDDERGTMTALIAHGHHTDGWNAPGR
                     DRLGKFSTWLGNTIIDTPGLTLPEGLPPAGVAELLRAEGPRDRLIELHPLIGATTDYD
                     SLDEELLFASMRRERLLDTWLILGHTHVPMQGPGSRFGGRWDRYVNGGSGIGERLITG
                     IEWDGSLDDPRPVLVGWTPGGTGQGSPERFEIVPTR"
     gene            4688..>5809
                     /locus_tag="PLP95_03790"
     CDS             4688..>5809
                     /locus_tag="PLP95_03790"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HPB44953.1"
                     /translation="MSASPRRRDQVGTDSVRTVVYYTDAAQIGGAETVARTLLAELDD
                     RFEVTIVGSDASVVDHIAAVRPSANTVLVPPITDRRDVAAMVKLRRVLRALRPDIFHA
                     NLSEGSSCQYGLLVALSIPGLRVVATENSPMGVRSGLSRRIKRFSAPRFDAHIGVGRR
                     AAALVEADVGLASGAVEVIPNAVPVVEHRSPAHRAPAGSDSPVRIVAISRFDPVKGLD
                     VLFDAVALLDRGSLSPFEVVVIGDGPQRGELEAHIDRLGIRDLVTLVGWVDDARQELV
                     DFDLFVLPSRLEGLPMSLLEAMHARVAVVATDVGSVREVLDSDAVGVVVAPGDAPALA
                     EAIGALIDDGQRRHGLAAAGQARALEQYTSAVNVAAYAAV"
BASE COUNT          868 a         1722 c         2175 g         1044 t
ORIGIN      
        1 cccacggccg ggaggggagc cgtgggcggt tcgcgatctg gccgatccgc caccgtccgg
       61 cggggtctga cgggacccct cgccacaact gctccggctc gtcgagcgtc accggtgcgt
      121 gtggggtcgt ggactgtggc ggaggtgcag cgggtgagat caggccggct gcaggtggcg
      181 gaggtgttcg gggaacggga tctgaggcat ctggtcctca gggcgtggag tcttcggggg
      241 tcgccctcgc cgacgcttgg tcatcacgac ctttccggcg acgatcaact gccctcccca
      301 gacgccccac tgctcttgac gggcaagtgc ggtgtcgagg cactcggaga tgacggggca
      361 ggtggagcag atcgttttgg cggcgatgat gtcggacaga tcatcggaga agaagagtcg
      421 agcggtaccc ggggtggcag cggcgcacgc ggcgtcgagg tgccaggagg tatcgggttc
      481 gagggtgagc ggaagtgcgg acatttgggt tcgaatcctt tcgccaggtt cccctggcag
      541 gtcaggtgct acgtagctgg gcgtcgtggc ggcgcttggc cgcaccggcg gtcgaggtgg
      601 tcgacgtggt cgtggcgaga tggggattcg aggtgggata catgggatgt ccttctgggt
      661 gttggtaggg gtgtgggagt gggcgggcgg tggctgcggt gaaggtgtcg tgaaacgccg
      721 aacgggccgc tggagtgttc caggcggccc gttcggagaa tcttgtttcg ctgtcgagtg
      781 cgttactcga tgcggtgatc ctcaagggcc gacagattgc acttggtcgc ataggcggcg
      841 caggtggcaa tgcgggtcgc cggagtggcg atggctgcgg tgcccgactg ccaaccgtgg
      901 cgcacagcac ggcaggcgag atgcgacacc cgaacatgcg cgcgcgacga agtcgtggct
      961 tcgccggtca tgctgttgtt cgttgcggtg cgcattgccg tgcgtccttc gggccctgag
     1021 gtgggttcag cggtgggttc agcgttggct gaatgtgggt tccagtatgg cggagttcgc
     1081 tgcgaaaccg gtgtcaaggg caccacaccg ctgcggaacc cgtcaaacga atttcgcaga
     1141 agattccaaa agtccggtct cacacggtga tttcgtagac cgggaacccg tcgcggtggt
     1201 ggcgggttgc ccggccgaca ttcacgagat gttcgaggtg ggcgtaggtc tccgattcgg
     1261 ccatcgaccc ccacgagcgt tcggcgaaca gctcgcgtga aaaggcctcg accgtggcga
     1321 tgccgagggt tgcgccgatg cgttcgatgc gttcgagacg atcgatgtga tgacgttcga
     1381 tggcgtcgac tcggccgacc aggtcggtga aggggtgccc gtgggccggc agggtgagcg
     1441 tgatgtcggg aagggtgcgc acgagatcga gagcgttgaa gaattcggcg agggcgtcgg
     1501 tgtgggaacg caggccggag atgtggggag tgatcgtcgg caggacgtga tctccgctga
     1561 acaagatgcc ctcggccaga tcgaccaggc ataggtgatc accggtatgg ccgggggtgt
     1621 gcaccgccac ccattcgcga cgccccaagc gaatgacgtc gtcgtggtcg aggcggacgt
     1681 tgggctcgtt gtgaatgaag ccccagcggg tatcggcatc ccaggaccga actacctcga
     1741 tctcggtgcg actcggcatc ggaaccgaac cgccccacgg ggcgcgcaac gctccgaggt
     1801 cggggatggg gccgtcgccg agcaccgcct gttcgagcgc gccgcgaagc tcctcgaagc
     1861 tcgcgcccgg tgcggcatcg gcgagttgga gttcggtgtc gtcgtcgccg tcgaagtggg
     1921 tggcgaaatg ccggtgggcg acgatgtcgg cctcgttggt gtagcggagg cgcccggccc
     1981 cgccgaagtg gtcggggtgg ctgtgggtga caaagaccgt gtggatccgg ctggtcggcg
     2041 cgccgatctg tgcgaagcgt tgctcaagtg cggcagcggt tgccgggtcg ggaaagccgg
     2101 ggtcgatgac ggtgaagccg cgctcgtctt cgagggcgta acagttcacg tggccgaggc
     2161 cgggcaggtt gatcggcagt tgcagacggt agataccctc ggcgacagcg gtgacctcct
     2221 ccgatgccgg gagccgctcc tggcgctcga tctcggtcat aggttcggct cggttgccgc
     2281 ggcgaaaacg ttgtctcgcc agtacttcag ttcggcgacg ctttctcgaa tgtcgtccat
     2341 cgcccggtgg gcggtcgcct tgtgtggcgc cgccgcgagc gcctcgggat accaccggcg
     2401 ggcgagttcc ttgatggtgg acacgtcgac cgagcggtag tggagatggt tctcgatctg
     2461 tgggagatag gcgttgagga agcgtcggtc catgccgatc gagttgccgc acagcggaac
     2521 gctgcgtggc tgaggggcgt gttccttgat gaacgccagg gtttcggcgc cggcctgttc
     2581 gagggtgatc tccgacgagc ggatggcgtc gagcaggccg ctggaggtgt gcatgttgag
     2641 cacgaccttg tccatgttcg cgagcgcttc gggcggctgg tggatcacca gatccggtcc
     2701 ttcggcgacg atgttgaggt cgtcgtcggt gatcagggtc gcgatctcca cgatgacgtc
     2761 ggttcggtcg tcgagtccgg tcatctccag gtccatccac acgagcatgg gctggaggct
     2821 accggcaccg gttaagaatc gccgttccgg cgctcgggta gggtcgtcgc cgtggaaatt
     2881 ccgttgatgc gtgtcgaccc gtccatcgag gtgcctgagg cggcgaagcc tggcgatgcc
     2941 ggcgtcgacc ttcgttcgac catcgacgtg gtcatcgccc cgggagggca ccgcgagttg
     3001 gtgccgaccg gcgtcgccat cgcgattccc gacgggtatg cggggttcgt tcagcctcgc
     3061 agcgggttgg ccctcaagca cggggtgacc gtgttgaaca ccccgggcct catcgattcg
     3121 gggtaccgcg gcgagttgaa ggtggtgctg gtcaacaccg acccctccga accgttccac
     3181 gtcgtgcgcg gagagcgcat cgcccagttg gtgattcagg cggtcgaacg tccggtgttt
     3241 gtggaagtgg cttcgctcga cgacaccgac cggggcaccg gagggttcgg tcacaccgga
     3301 cgcggttagc gggtcgggac gatctcgaac cgttccggac tgccctggcc cgtgccgccc
     3361 ggagtccaac cgacgaggac cgggcgcggg tcgtcgagcg atccgtccca ctcgataccg
     3421 gtgatcaggc gctcgccgat accgctgccc ccgttcacgt agcgatccca acggccgccg
     3481 aaacgtgagc cgggcccctg catcgggaca tgggtgtggc cgaggatcag ccatgtgtcg
     3541 agcaggcgtt cgcggcgcat cgacgcgaac agcagttcct cgtcgagcga gtcgtagtcg
     3601 gtggtggcac cgatgagcgg gtgcagttcg atgaggcgat cgcgtgggcc ctcggcgcga
     3661 aggagttcgg caaccccggc gggcggcaat ccctcgggca gggtgaggcc cggggtgtcg
     3721 atgatcgtgt tgcccagcca ggtcgagaac ttgcccaggc ggtcgcgtcc gggagcgttc
     3781 cagccgtcgg tgtggtgccc gtgggcgatg agtgccgtca tcgtgccgcg ctcatcgtcg
     3841 agggtgatga agtcggccag cgggaacggt ccgagccgat cggtgagcac tcgggcgacc
     3901 tcgtggtgat gcagcgggtc gtcgtggttg ccgaccgtgc gcaggtatcg accgtccgca
     3961 gcgaagcgcg atgcgatcac cgagtagatc tcgtcgttgt tgtcgatgat cgcggccagg
     4021 tattgccgat agagcgcggt gctcaggcgg tcgtcgccga gcgcccctcc gaggagccgc
     4081 agtgcgtcat aggtcgcgcc aagcgtcgac ccgccgacca tccagaagtc ttcgacgtcg
     4141 ccgttctcgc acaaggtcca gccgtcgtcg gcgtagcggc gcagcacctc cgcatagagc
     4201 gacttggtgc cctggcgtcg gggccagtcg agacgcccgg ggacgcaccg atgcaggtcg
     4261 gagaagatga cgatgcgttc gctgtgggga cggcgcacga cgacgtcggc gccggtgctc
     4321 ggcgtccggc cacgcgagat gctcgacacg gcggtcggaa actcgcggat gtctcgcttg
     4381 gctcgccggt ggtgggtgtt cgcgacgaac agggcgaacg gaacccacag cggtgcgccg
     4441 actgcgagga cgaggaattt gagcaatgcc cacagtgtgg cgagtgcgcc gattcgcgca
     4501 gactggtcgt gggcgcgccg tcggtcgatg gccttggccc accgcaggat cgattcgata
     4561 cgttcgtcga ggggttcagc ggccaacgcc atggtctcag tgtcgcgctc gcggacgtgg
     4621 cgcaccggaa tcggcgcctc ctctggcaca ctgtcgagcg ccccgcccgc cgatcacgga
     4681 gtgttccatg agtgccagtc cacgtcgccg tgaccaggtg ggaaccgact cggttcgcac
     4741 cgtcgtctac tacaccgacg ccgcgcagat cggcggggcg gagacggtgg cacgcacgct
     4801 gctcgccgag ttggacgatc ggttcgaggt caccatcgtc gggtcggacg cgtcggtcgt
     4861 cgaccacatc gcagccgtgc gaccgtcggc gaacacggtg ctggtgccgc cgatcaccga
     4921 tcgccgcgac gtcgcggcga tggtgaagtt gcgccgagtg ctgcgggcgc tgcgacccga
     4981 catcttccac gcgaatctga gcgagggatc ctcgtgtcag tacggcctgc tcgtggcact
     5041 gtcgattccg ggcctgcgag tcgtcgccac cgaaaactct ccgatggggg tccgttcggg
     5101 gttatcgcgc cggatcaagc ggttctcggc tccgaggttc gatgcgcaca tcggggtagg
     5161 ccgtcgggcc gcggcactgg tcgaggccga cgtcgggttg gcttcggggg ccgtggaggt
     5221 gatcccgaac gcggtgccgg tggtcgaaca ccgctcgccg gcccaccgtg cgccggcagg
     5281 gagcgacagc cccgtgcgca tcgtggcgat ctcccggttc gatccggtca agggcctcga
     5341 cgttctgttc gacgcggtgg cgctgctcga ccggggctcg ctttcgccgt tcgaggtcgt
     5401 cgtgatcggc gacggaccac aacgcggcga actcgaggcg cacatcgatc gtcttggcat
     5461 tcgcgacctc gtcacgctgg tcgggtgggt cgacgacgcc cgacaggagc tggtcgattt
     5521 cgatctgttc gttttgccgt cgcgcctcga aggcctgccc atgtcgctgc tcgaggccat
     5581 gcacgccagg gtggcggtgg tcgccaccga cgtcggcagc gtgcgcgagg tgttggactc
     5641 cgacgcggtg ggcgtcgtcg tcgccccggg ggatgcaccg gcgttggccg aggccatcgg
     5701 cgcgttgatc gacgacgggc agcgtcggca cggccttgcc gccgccgggc aggcccgtgc
     5761 gctcgagcag tacacgagcg ccgtgaacgt cgccgcctac gccgcggtg
//
LOCUS       DAOOMA010000089         5805 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_16537_length_5805_cov_3.812696, whole genome shotgun sequence.
ACCESSION   DAOOMA010000089 DAOOMA010000000
VERSION     DAOOMA010000089.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 5805)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5805)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5805
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16537_length_5805_cov_3.812696"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..552)
                     /locus_tag="PLP95_03795"
     CDS             complement(<1..552)
                     /locus_tag="PLP95_03795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020378226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HPB44954.1"
                     /translation="MSDSVGIYLNQIGTVPLLTAAEEIELSKAIEAGADARARKEAGE
                     RSAEINRKIAAAAAAKDRFIRANLRLVVSVARRYPLPPSMELLDLVQEGNLGLEHAVD
                     KFDWRKGFKFSTYATFWIRQSIGRALDQKASLIRLPGDRSASLRAALRAVSGDGDELD
                     SENALLHRLSTPTSLDRSVGHDES"
     gene            799..1419
                     /gene="nadD"
                     /locus_tag="PLP95_03800"
     CDS             799..1419
                     /gene="nadD"
                     /locus_tag="PLP95_03800"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: protein motif:HMM:NF000840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HPB44955.1"
                     /translation="MTTQRVAVFGGTFDPPHLGHLIVATEVGFRGGFDEVILMVANDP
                     WQKSPQRSVTPAADRLDLVAAAIAEDTDRYCGLRAGHEEVRRGGATYTIDTVEAMRRA
                     RPGVEVSLVLGADAASRLESWHRVDDLRGLVELTVVGRPGAPTPVLDPRWRCTVVDVP
                     QIEISSTEIRRRVAEGIPYAHLIPGAVVQLIRSRGLYGLGMSGTQS"
     gene            1416..2624
                     /locus_tag="PLP95_03805"
     CDS             1416..2624
                     /locus_tag="PLP95_03805"
                     /inference="COORDINATES: protein motif:HMM:NF024791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytR C-terminal domain-containing protein"
                     /protein_id="HPB44956.1"
                     /translation="MTDAVSVAPRPAPRRRRVLSSVFLVITLLAVGGFAGLAWYGVDA
                     ARNVIGGTSAEVITDPQAPGFLAVVNETRVSLVVLIGADGQLDSTLLFPEPTDDGSAS
                     VVWTLGDLLVAGDDGEERTLAQIHAGDPAEGDGGTDGTGGTAAVAEAMESVLGFRISD
                     VTEATVEDIESIAAILAPVTVRNPSSVSVSDGSETTVRYRSGELELSASEVADFVTVR
                     GAGEAPEDRGLRVKATLEALGAALSDLGLEDTGEDPRDLGAFLIRWSMADHSFVSLPV
                     DPVAFEGSYLYRPNGLAIIDELSGLVPFPASAFAGQRPLARILNGTTDTDAASRVASQ
                     VAALGAEIATVGNAAAFDADATTVLYSDEAFAPVAERIAEFLGAEATRVEEVSDATDI
                     DIELGADLAL"
     gene            2637..3035
                     /gene="rsfS"
                     /locus_tag="PLP95_03810"
     CDS             2637..3035
                     /gene="rsfS"
                     /locus_tag="PLP95_03810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HPB44957.1"
                     /translation="MTLEDPTADPIRSMAVAAARAADNKVASDIVVLEVGPIVSIYEY
                     LVIASATNPRQVAAVAQEIEDVLALEFDRRPNSVEGRDTGRWILADYGDIVVHVFHTD
                     ERDFYRLERLFGDAARVDWQPAPIGLGDPA"
     gene            3053..4009
                     /locus_tag="PLP95_03815"
     CDS             3053..4009
                     /locus_tag="PLP95_03815"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HPB44958.1"
                     /translation="MSAAVQIDGYLVGEVLGRGGSATVYDAARVGDGRRVALKVLDHR
                     VRRGERELAALVAIGEVPGVIEVLEVVETAQGATCLVLERMHESAAARLQREGPFSVV
                     EVVRIGRVVGAALEGMHRLGVAHRDVKPGNVLLGEGMGSDLEVRLADFDIALLIDVEA
                     STETVGSLTPPHAAPERFTGSDSGSPAADIWSLGSTLFTLLHGEPPFGGARDDGGVAG
                     LAHRVMSEPSAALMRSDVPAGLVAVIDRCLAKDPLDRWASAGDVVTALDGLDGVDGGR
                     DGPVEPESSVLVDGSTTTTAMTWAIWAAGVAVGLFVLWLALR"
     gene            complement(4031..4747)
                     /locus_tag="PLP95_03820"
     CDS             complement(4031..4747)
                     /locus_tag="PLP95_03820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020379361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HPB44959.1"
                     /translation="METQIEEIADGIFRISTFVEMAGMRFNQYLIDAEEPLLFHTGQR
                     ALFPLVSKAFARVKPVDSLRWVSWGHLESDESGAMNEWLAASPQATVAVGFLAAVLSV
                     NDLADRPPRLLGDDEVIDLGGKRVRFLPTPHVPHGWDAGVMFEETTGTLLCGDLFTQH
                     GDTEAIDHGDLIGPAAAAEDAFGSTALTPNTAPTIAALAELEPNTLALMHGPAWHGDG
                     ATALRDLANFYAERLAASTG"
     gene            4906..5643
                     /locus_tag="PLP95_03825"
     CDS             4906..5643
                     /locus_tag="PLP95_03825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB44960.1"
                     /translation="MLEITLDRAVHRLHPGDVRTFGRGGAGVDYVIGDDDRLHRRAGT
                     ITVSESGWDLRNDGRWLLLRIVSAERFGLDVLEPGNSLHVPWKRARVEVVVGASTVGF
                     SAEFRGQNPENDPRPPSFVGDGDPGTVTPTSIERTSGAFRALVALCEPRLRQPGTLEV
                     ATDLEIARRLNAQGDEQRRITGKTVERRLDYCRGRLDLKLRGPGGSSIGSEQRNARRH
                     LAEVALMTGTVTVDDLEVLDATPDSFR"
BASE COUNT          850 a         1822 c         2094 g         1039 t
ORIGIN      
        1 cgactcgtcg tggccgaccg accggtcgag cgaggtcggc gtcgacagac ggtggaggag
       61 ggcgttctcg ctgtccagct cgtcgccgtc acccgacacc gcgcggagcg cggcgcgaag
      121 tgaggctgac cggtcgccgg gaaggcgaat gaggctggcc ttctgatcga gggcccggcc
      181 gatcgactgg cgaatccaga acgtggcata ggtggagaac ttgaacccct tgcgccagtc
      241 gaacttgtcg acggcatgct ccaggccgag gttgccctcc tggacgaggt cgagcagttc
      301 catgctcggc ggcagcgggt agcgtcgcgc cacgctcacg acgaggcgaa ggttcgcccg
      361 aatgaagcgg tcctttgctg cggccgccgc ggcgatcttg cggttgatct ccgcactgcg
      421 ttcgccggcc tccttgcggg cacgggcgtc cgctccggcc tcgatggcct tcgagagctc
      481 gatctcctcc gccgccgtga ggaggggaac cgtgccgatt tggttcagat agataccgac
      541 ggaatcgctc atgtcgtcac ctcgtgcttc cacgcacgtg ttgcgaccgg aaattttccg
      601 atcgcagggg tggaaactcg ctgtttcacc cctacgctga atgtcgaagc gacgaatcgc
      661 ctcaggtacc tatcggcaat gtggcccaga ggttgaggcg ttcgacgaac agctgtggtg
      721 aatttctcgt cgactcctcc tgaggagcgc tttggcgcgc tggcggtgtg gccgggtcga
      781 tcgctctacg atcgtgcggt gaccacccag cgagtcgccg tgttcggcgg gacattcgat
      841 cctccgcacc tcggccacct gatcgtcgcc acggaggttg gcttccgcgg cggattcgat
      901 gaggtgatcc tgatggtggc caatgaccct tggcagaagt cgccccaacg ttcggtgacc
      961 ccggcggcgg accggctcga cctcgtcgct gcggcgatcg ccgaggacac cgatcgctat
     1021 tgcgggttgc gggccggcca cgaggaggtt cggcgcgggg gagcgaccta cacgatcgac
     1081 accgtcgagg cgatgcgcag ggcccgcccc ggggttgagg tgtcgttggt gctcggcgcc
     1141 gatgcggcat cgcggctcga gtcatggcat cgcgtcgatg accttcgagg actcgtcgag
     1201 ttgaccgtcg tcggccgacc cggcgcgccg accccggtgc tggatcctcg atggcgttgc
     1261 acggtggtcg atgttccgca gatcgaaatc tcgagtaccg agatccgccg tcgggtcgcc
     1321 gagggcattc cctacgcgca tctcatcccg ggggcggtcg tccaactgat ccgctcccgt
     1381 ggcctctatg gtttgggtat gagcggtacg cagtcatgac cgacgctgtc tccgtcgcgc
     1441 cgcggcccgc tccgcgccgt cgccgcgtgc tgagttcggt gtttcttgtc atcacgctgt
     1501 tggcggtcgg cggattcgcc gggctggcgt ggtacggcgt ggatgcggct cgcaatgtga
     1561 tcggtggcac ctcggcggag gtcatcaccg accctcaggc gccggggttt ctcgccgtgg
     1621 tgaacgagac ccgggtcagc ctggtcgtgc tcatcggagc cgacggacag ctcgactcga
     1681 ccctgttgtt tcccgaaccc accgacgatg gttcggcatc ggtggtgtgg accctcggcg
     1741 acctcctggt ggccggagac gatggcgagg aacgcacgct cgctcagatt catgcgggtg
     1801 acccggccga aggggacggc ggcaccgacg gcaccggcgg caccgcggcg gtggcggagg
     1861 cgatggaatc ggtgctcggc ttccgcatca gcgacgtcac cgaggcgacc gtcgaggaca
     1921 tcgagtcgat cgccgccatc ctcgcgccgg tgacggtgcg aaacccgagc agcgtgagcg
     1981 tgagcgacgg ctcggagacc accgtgcgct accgaagcgg tgagctcgag ttgtcggcga
     2041 gcgaggtggc ggacttcgtg acggttcgcg gcgcgggcga ggccccggag gatcgtgggc
     2101 tgcgggtgaa ggcgacgctc gaagcgttgg gtgcggcgct gtccgatctc ggcctcgaag
     2161 acaccggcga ggacccgcgg gatctgggag cgttcctgat ccgctggtcg atggccgacc
     2221 attcgttcgt gtcgctgccg gtcgacccgg tcgcgttcga aggctcctat ctctaccgtc
     2281 cgaacgggct cgccatcatc gacgagttgt cggggctggt gccgttcccg gcgagcgcgt
     2341 ttgcgggaca acgcccgctg gcgcggatcc tcaacggcac caccgacacc gatgccgctt
     2401 cgcgggtggc ctcgcaggtt gctgcgctcg gcgcggagat cgccacggtc ggcaacgccg
     2461 cagcgttcga tgccgacgcc acaaccgtcc tatattcaga cgaggcgttt gcgccggtgg
     2521 ccgaacggat cgccgagttt ctcggtgccg aggccactcg cgtggaggag gtctccgacg
     2581 ccacggacat cgacattgaa ctcggtgccg atctcgcatt gtgagtgtgt gaacccatga
     2641 cccttgaaga tccaaccgca gatccgatcc gatcgatggc ggtcgcggcg gcccgggcgg
     2701 ctgacaacaa ggttgcgagc gacatcgtgg ttctcgaggt cggaccgatc gtgtcgatct
     2761 atgagtatct cgtgatcgcg tcggccacca accctcgaca ggtcgccgcg gtcgcccagg
     2821 agatcgagga cgtgttggcc ctcgaattcg atcggcgtcc gaattcggtg gagggacgcg
     2881 ataccggccg gtggattctc gccgactacg gcgacatcgt cgttcacgtg ttccataccg
     2941 acgagcgcga tttctaccgc ctcgagcgcc tcttcggtga tgcggccagg gtcgactggc
     3001 aaccggcacc gatcgggctc ggcgaccccg cgtagccgac gctgaggcga ggttgagcgc
     3061 ggcggtgcag atcgacggct acctggttgg cgaggtgctc ggccgtggcg gctcggcgac
     3121 ggtgtatgac gcggcccggg ttggcgacgg gcgccgggtg gcgttgaagg tgctcgacca
     3181 ccgggtgcgc cggggcgaac gcgaactggc ggcgctggtc gcgatcggtg aggtgccggg
     3241 ggtcatcgag gtgctcgaag tggtcgagac cgcgcagggc gcgacctgtc tggtcctcga
     3301 gcggatgcac gaatcggcag cggcgcggct gcagcgcgag ggcccgttct cggtggtgga
     3361 ggtcgtgcga atcggccggg tggtcggcgc cgcgctcgag gggatgcacc gcctcggtgt
     3421 ggcccatcgc gacgtgaaac ccggcaacgt cttgttgggc gagggaatgg ggtcggacct
     3481 tgaggtgcgg ctcgccgact tcgacatcgc gctgttgatc gatgtggagg cgtcgaccga
     3541 gaccgtcggg tcgctcaccc cgccgcatgc ggcacccgaa cggttcaccg gttcggattc
     3601 cggcagtccc gccgccgaca tctggtcgct cggttcgacg ttgttcacgc tgttgcacgg
     3661 tgagccgccg ttcggcgggg cgcgggacga cggtggggtt gccgggttgg cacaccgggt
     3721 gatgagcgag ccgtctgcgg cgttgatgcg cagcgacgtt cccgccggcc tcgtggccgt
     3781 catcgaccgg tgcctggcga aggacccgct cgatcggtgg gcgagcgccg gggacgtggt
     3841 gaccgcgctc gatgggctcg atggagtcga tggtggccgt gatgggccgg ttgaaccgga
     3901 gtcgtcggtc ctagttgacg gctcgaccac gacgacggcg atgacctggg cgatctgggc
     3961 cgcaggagtt gccgtcgggc tgttcgtgct ttggttggcg cttcgctgag ggtcggtggg
     4021 gagcggtgag ctaaccggtg cttgcggcga gtcgctcggc gtagaagttc gccaggtcgc
     4081 gcagcgcggt ggcaccgtcg ccgtgccacg cggggccgtg catcagcgcg agggtgttgg
     4141 gttcgagttc cgccagcgcg gcgatggtcg gcgccgtgtt cggggtcaat gcggtggatc
     4201 cgaacgcatc ctcggccgcc gctgcgggcc cgatcaggtc gccgtggtcg atcgcctccg
     4261 tgtcgccatg ttgggtgaac aggtctccgc acaggagcgt tccggtcgtt tcttcgaaca
     4321 tgacgccggc gtcccaaccg tgggggacat gcggcgtcgg caggaatcgc acgcgtttgc
     4381 caccgaggtc gatcacctcg tcgtcgccga ggagccgcgg cggccggtct gcgaggtcgt
     4441 tgaccgacag gaccgccgcg aggaacccga cggcgaccgt tgcctgtggc gaggcggcca
     4501 gccactcgtt catggccccg ctctcgtcgg attccaggtg accccaactc acccagcgca
     4561 gcgagtcgac gggcttcact cgggcgaacg ctttggacac gagcggaaac agcgctcgtt
     4621 gcccggtgtg gaacaacaac ggctcctcgg catcgatgag gtactggttg aatcgcatcc
     4681 cggccatctc gacaaaggtc gagatcctga agatgccatc ggcgatctct tcgatttggg
     4741 tttccaaggt catcgcctcc ccggggaggc cggaacgcga cgctcccgat atggcgatgc
     4801 ttccacatcc gcgtcgcgga tgcgggggga agtccgcatg ggggatatcc cccattggcg
     4861 tcgtggccca cccgtgcttc gctgcccggg ttcttcggag gtcagatgtt ggagatcacc
     4921 ctcgatcgag ccgtccaccg gctccaccca ggcgatgtgc gcacgttcgg tcgtggcggc
     4981 gcgggcgtcg actacgtcat cggcgacgac gaccggctcc atcgcagagc gggcacgatc
     5041 accgtgagcg agtcgggctg ggatctgcgc aacgacggcc ggtggctctt gttgcgtatc
     5101 gtcagcgccg agcggttcgg cctcgacgtg cttgagccgg gcaattcctt gcacgtgcct
     5161 tggaagcgcg ctcgtgttga agtagtggtg ggagcttcca ccgtggggtt ctcggccgag
     5221 ttccgcggtc aaaatccgga gaatgatccg cgccccccgt cgttcgtggg cgacggagac
     5281 ccgggaacgg tgacaccgac gtccatcgag cgaacctcgg gagcgttccg ggcgctcgtg
     5341 gcgctctgtg agcctcggct gcgccagccc ggcacgctcg aggtcgccac cgacctcgag
     5401 atcgcccggc ggctcaacgc tcagggagac gagcagcggc gcatcaccgg aaagaccgtc
     5461 gaacggcgcc tcgactattg ccgtggtcgc ctcgatctca agttgcgcgg accgggggga
     5521 tcctcgatcg gttcggaaca acgcaatgcc cgtcgccatc tcgccgaggt tgccttgatg
     5581 accggaacgg tcacggtcga tgacctcgag gtgttggacg ccacgccgga ctcgttccgg
     5641 tagccggtga cggagcacaa cgtggtggtg gcgactcaac gccgcgccgc cgtcgtcgag
     5701 ttgagcgtct cgcaacgcgg gtcgaccggc gatgccgcga tcgtgaacct gggcgccttc
     5761 cgtgtcgaga tcgatgcgtc ggcgacggtg ctcgactggt gccgg
//
LOCUS       DAOOMA010000090         5764 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_16708_length_5764_cov_3.961114, whole genome shotgun sequence.
ACCESSION   DAOOMA010000090 DAOOMA010000000
VERSION     DAOOMA010000090.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 5764)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5764)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5764
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16708_length_5764_cov_3.961114"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(145..1050)
                     /gene="rpoD"
                     /locus_tag="PLP95_03830"
     CDS             complement(145..1050)
                     /gene="rpoD"
                     /locus_tag="PLP95_03830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016291595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HPB44961.1"
                     /translation="MYLREIGQVELLTGPEEVALAKRIEAGVFAESRLADLAASGEEI
                     DLAERRKLQRVVRDGERAKTELTQANLRLVVSIAKRYLGRGLAILDLIQEGNLGLMRA
                     VEKFDYSKGFKFSTYATWWIRQAVTRAIADQARTIRIPVHMVESMNKVHRHQRQLMQE
                     LEREPTIEELAAKVGLAPARVRDILLISQDPLSLDSPVGEEEDSLLADFIEDSSAIAP
                     ADAAAKQMLGQALIEALDDLNPRERQVVRLRFGLEDGQAKTLEEVGREFGVTRERIRQ
                     IESKTLAKLRNPHRSQKLRDYLDVE"
     gene            complement(1530..3488)
                     /gene="dnaG"
                     /locus_tag="PLP95_03835"
     CDS             complement(1530..3488)
                     /gene="dnaG"
                     /locus_tag="PLP95_03835"
                     /inference="COORDINATES: protein motif:HMM:TIGR01391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HPB44962.1"
                     /translation="MGIADEDIVEVRDAADIVAIINQHTTLRRVGQRWTGLCPFHNEK
                     SPSFSVNQNEGLFYCFGCQKSGDVITFVREIEHLDFVGAVEWLAAKVGIVLTYTDRDE
                     GAGRRRQKQLHDVMERAVEWYRARLNAPGPDAGAARRYLRSRGFDREMVEQFQVGWAP
                     DSWDELCRFLKVPDDVLVDCGLGFRNKASRQQDFFRARVLFPIFDDQGRPVAFGGRKL
                     PDTDGPKYQNSRETKLYNKSATLYGLNWAKADIVNANEAVICEGYTDVIGFFRAGIPR
                     AVATCGTALTEDHIRSLKRFTRRIILAYDADEAGQNAAEKVYAWEQTHDIEVHVLSLP
                     KGADPDELSRENPEALADAVSTAKPFLAFRVQRVLDAADVATPEGRARAASAALDVIA
                     EHPSPFLRDQYLMEVAGFTRVDEHQLRTQLAAGPRPKPQVETQQRRRRGETGNGSDEP
                     PPWVGSSGNHGFGDDGFGGDGYGDDGAGIGHGGGSAPASNVAPTAGSQRRSSAPLDGP
                     QLEALRMIIHRRYEIEEWLRPVLFGDVRARAVYESIAVHPDLASAIAAIDERGDPELS
                     GLVARLSVEETEAPVDDVVSLLLVDAAMRASSRFEAEARTSEDPLAYAPLISWLKLRL
                     DSLRGDEASMEFAPELLAWLDENSAELS"
     gene            complement(3488..5026)
                     /locus_tag="PLP95_03840"
     CDS             complement(3488..5026)
                     /locus_tag="PLP95_03840"
                     /inference="COORDINATES: protein motif:HMM:NF014895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-utilizing enzyme"
                     /protein_id="HPB44963.1"
                     /translation="MDTTPLSAIEAAVGGAGDEPTPAARLAALNSISPAMIDDVVFGA
                     DAARLGAAADGRVVAIGVAASPGVGVGRVAVDPVAALDSWEAGRDVVLVVDETTPADE
                     PAMRVASAIVTRRGSLASHAAIIARQYGIPAVCGVGEIEARAGEQWLVDGATGEVRHL
                     DDDSASQAGRGGPALSELPTAVKTLLAWADEVAAGGLVVMANADDENAVRTALSLGAS
                     GVGLCRTEHQFLGEAAGVVARLLAGDHEALAEFAQHQRAALRGVFSAAEDRPITVRLL
                     DAPSHEFGGATEHNPMLGVRGIRLGLMRPEILEAQAVAAAEAWCDVAEPSSAGRSAAP
                     RVRLCLGVVAFPEEVAAARSIIDGAVERVGSVRGRRIDMAASAMVETPRAALCTAMFA
                     TEVDSICFGTNDLTQLTYGLSRDDSAELVAEYRRRGILERDPFATLDHLGVARLLALA
                     AQTATAANPEIALSMCGEHAADPASIAIAAQLGITTLSVSPYRVPATRLAAARCIIEG
                     DGRR"
     gene            5153..>5764
                     /locus_tag="PLP95_03845"
     CDS             5153..>5764
                     /locus_tag="PLP95_03845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB44964.1"
                     /translation="MSEESRLKDDGMVIFPGPPDLGTIRNPPHRHHQGDCRHMTTIDL
                     PGLQRSTSVLDQIRDGVSFGLLFGGQGGDYRPRVEALIGTHRTEIAAPSLWGDALARL
                     DARLDTMLAWYPRDPRSAHLPDPPRPTTPRLHPERSDPERAHPARSHPARSHPARSHP
                     ALSVPGIAFAQLLERRALRLHGIGGSGRHDTEPAPSVAIGHSQG"
BASE COUNT          935 a         2028 c         1836 g          965 t
ORIGIN      
        1 cgacccactg attaagagtc agttgctctg ccagctgagc taccccggat tgtgctgtgc
       61 gccgcaacac acggatgaac actctagcag tggcgcaacg agtcgacgca catcggtttc
      121 gctcggccgg tcaccggcga ggacctactc gacgtcgagg taatcgcgca gcttctggct
      181 gcggtgcggg ttgcgcagct tggccagcgt cttcgactcg atctggcgga tacgttcccg
      241 ggtcacgccg aactcgcgtc cgacctcctc gagcgtcttg gcctgaccgt cttcgaggcc
      301 gaatcgcaat cgcacgacct gacgctcacg cggattgaga tcgtcgagcg cctcgatcag
      361 cgcctggccc agcatctgct tcgcagcggc atcggccgga gcgatcgccg aggagtcctc
      421 gatgaagtcg gcgagaaggc tgtcttcctc ctcgcccacc ggagaatcga gcgacagcgg
      481 atcctggctg atgagcagga tgtcgcggac ccgggccggg gcgaggccga ccttcgccgc
      541 gagctcctcg atggtgggtt cgcgttcgag ttcctgcatc aactgacgct ggtggcgatg
      601 caccttgttc atcgattcga ccatgtggac cggaatacga atggtgcggg cctgatcggc
      661 gatcgcccgg gtcacggcct ggcggatcca ccacgtggca taggtcgaga acttgaaccc
      721 cttcgagtag tcgaatttct cgaccgctcg catcaatccg aggttgccct cttgaatcag
      781 gtcgagaatc gcgagccctc ggccgaggta ccgcttggcg atcgacacga cgaggcgcag
      841 attcgcctgg gtcagctccg tcttggcccg ctccccgtcg cgaacgacgc gctgcaactt
      901 gcgccgctcg gcgaggtcga tctcctcccc cgatgccgcg aggtcggcga ggcgcgactc
      961 ggcgaacacc ccggcctcga tccgcttggc gagcgcgact tcctcggggc cggtcaacag
     1021 ctcgacctgg ccgatctcac gcagatacat gcgcaccgga tccgacgagg ttcccaccgg
     1081 cgtcgccgac gtcggcaacg tgcgggtggc gaaacgacgt accggtggtt cgacctcggg
     1141 gtcctcgggc tgcggtgtgg cggcggtcgt gccggtcgcg ccggtcgctt catcggaacc
     1201 ggagacggcg acgtcacccg cctcgggctg cgtctgcttc ggcgccgctt cgaacaaccc
     1261 ctcgaggttg acgtcgtgca gatcgacggc ctcttcgatc cgtacgccca tcggggtgaa
     1321 gtaggtgcgg atctcctcga ccacgtcgac ggtcagttcg acgccgagca ccgacaacac
     1381 gtcgtcgatc ggcgccacgc ccgaggcttt cgagcgctcg atgagtgcag ccaggtctgc
     1441 ctctggaact tcgtcgaaga gtcccacgct cgatcctttc tgcacagtcc attcatcggc
     1501 agcgacgtcc atcgcataca tctgcgctgt catgacaact ccgcggagtt ctcgtcaagc
     1561 caggctagca actcgggagc aaattccatg ctggcttcat cgccacgcag agagtccaac
     1621 ctgagtttca accaggaaat caacggggcg tacgcgagtg gatcctccga ggtgcgtgcc
     1681 tcggcttcga atctggagct ggcgcgcatg gcggcgtcga ccagcaacaa cgacacgaca
     1741 tcgtccaccg gggcctcggt ctcctcgacc gagagacgcg ccacgagtcc gctgagttcc
     1801 ggatcgccgc gctcgtcgat cgcggcgatc gccgaggcca ggtcggggtg aacggcgatc
     1861 gactcgtaca cggcgcgggc gcgaacgtca ccgaacagga ccggacgcag ccactcctcg
     1921 atctcataac ggcggtggat gatcatcctc agcgcctcga gttgtgggcc gtcgagcgga
     1981 gcggaggacc gccgctgcga acccgcggtc ggcgccacat tcgaggccgg tgccgaccct
     2041 cccccatgtc cgatgccggc accgtcatcg ccatagccat caccaccgaa gccatcatcg
     2101 ccgaatccgt ggttgcccga ggacccgacc cacggcggcg gttcgtctga accgttcccc
     2161 gtctcgcctc gccgacgcct ctgttgtgtt tcgacctgcg gcttgggtcg tggccctgcg
     2221 gcgagttggg tgcgcaactg gtgctcgtcg acccgcgtga acccggcgac ctccatgagg
     2281 tactggtcgc gcaggaacgg cgacgggtgc tcggcgatga cgtcgagtgc cgccgaggcg
     2341 gcccgggcac gcccctcggg ggttgcgacg tctgcggcgt cgagcacgcg ctgcacgcgg
     2401 aaggcgagaa acggtttcgc cgtggacacc gcgtcggcga gcgcctcggg gttctcgcga
     2461 ctcaactcgt cgggatcggc ccccttgggc agcgacagaa cgtgcacctc gatgtcgtgg
     2521 gtctgttccc acgcgtacac cttctccgcc gcgttctgcc cggcctcatc cgcgtcgtag
     2581 gccaagatga tgcgtctcgt gaaccgcttc agcgaccgga tgtgatcctc ggtcaacgcg
     2641 gtcccacacg tcgccacggc cctcgggata ccggcacgaa agaacccgat gacgtcggtg
     2701 tagccctcgc agatgaccgc ctcgttcgcg ttgacgatgt cggcctttgc ccagttgagt
     2761 ccgtacagcg tcgcgctctt gttgtagagc ttggtttcgc gcgagttctg gtatttcggt
     2821 ccgtcggtgt cgggcagttt ccgaccaccg aaggccaccg gacgcccttg gtcgtcgaag
     2881 atgggaaaca gcacccgggc ccgaaagaag tcctgttgac gactcgcctt gttgcgaaac
     2941 ccgagcccgc aatcgacgag cacgtcgtcg ggcaccttca ggaaccgaca cagttcgtcc
     3001 cacgaatccg gagcccaccc gacctggaac tgttcgacca tctcccggtc gaacccacgg
     3061 ctgcgcaggt agcgccgcgc cgctcccgcg tcggggccgg gggcgttgag ccgggcccgg
     3121 taccactcga cggcgcgttc catgacgtcg tgtaactgct tttggcgccg ccgtccggcg
     3181 ccctcgtcac gatcggtgta ggtgaggacg atgccgacct tggccgccaa ccactcgacc
     3241 gcgccgacga agtcgaggtg ttcgatttcg cgcacgaagg tgatcacgtc gccggacttc
     3301 tgacatccga agcagtagaa gagcccctcg ttctggttga ccgagaacga cggactcttt
     3361 tcgttgtgga acggacacag gccggtccag cgctgcccga cgcgccgcag ggtcgtgtgc
     3421 tggttgatga tcgccacgat gtcggcggcg tcacggacct cgacgatgtc ctcgtcggcg
     3481 atacccatca gcggcgaccg tcgccttcga tgatgcaccg ggccgcggcc agccgcgtcg
     3541 ccggcacccg atacggcgac accgacaatg tcgtaattcc gagctgtgct gcgatggcga
     3601 tgctggcggg gtcggcggcg tgttcaccac acatcgataa cgcgatctcg gggttggcgg
     3661 cggtcgccgt ctgagcggcc aacgccagca ggcgggcgac cccgaggtga tcgagggtgg
     3721 cgaatgggtc gcgttccagg attccccgac gccggtactc cgcaaccagt tcggcggagt
     3781 cgtcgcggct cagcccgtag gtgagctggg tgaggtcgtt ggtgccgaaa cagatcgagt
     3841 cgacctcggt ggcgaacatc gcggtgcaca gcgccgcacg tggcgtctcg accatcgccg
     3901 aagccgccat gtcgatgcgt cgaccacgaa ccgaacccac ccgctcgacc gcgccatcga
     3961 tgatcgaccg ggccgcggcc acctcctcgg ggaaggccac gacgccaagg cacagcctga
     4021 ccctcggcgc cgccgatcgg cccgcgctcg aaggttccgc aacgtcgcac cacgcttcgg
     4081 cggcggccac cgcctgcgcc tcgagaatct cagggcgcat caggcccagc cggataccac
     4141 gcactccgag catggggttg tgctcggtgg ctcccccgaa ctcatggctg ggggcatcga
     4201 gcagccgaac cgtgatcggg cgatcctcgg ccgcggaaaa gacgccccgc aacgccgcgc
     4261 gctggtgttg ggcaaactcc gccagcgcct cgtggtctcc ggcgaggagc cgggcgacga
     4321 cgccggccgc ctcgccgaga aactggtgct cggttcggca cagtccaaca ccgctcgccc
     4381 cgagggagag cgccgttcgc accgcgttct cgtcgtcggc gttcgccatg acgacaaggc
     4441 ctccggcggc gacctcatcc gcccaggcca acagcgtctt caccgctgtt gggagctcgc
     4501 tcaacgccgg tccacctcga ccggcttggg aggccgagtc gtcgtcgagg tgtcggacct
     4561 cgccggtcgc cccgtcgacg agccactgct cgccggcgcg ggcctcgatc tcaccgacgc
     4621 cgcacaccgc cgggatgccg tattggcgag cgatgatggc cgcgtggctt gcgagtgagc
     4681 ctcgcctggt cacgatggcc gaggccacgc gcatggccgg ttcgtctgca ggcgtggtct
     4741 cgtcgaccac gagcaccacg tcgcggcccg cctcccacga gtcgagcgcg gcgaccggat
     4801 cgaccgcgac gcgccccacc cccaccccgg gcgatgcggc gaccccgatg gccacgacgc
     4861 gcccatccgc cgcggcgccg aggcgagcgg cgtcggcccc gaacaccacg tcgtcgatca
     4921 tcgctggact gatcgaattc agtgcggcga ggcgcgccgc aggggttggt tcgtcgccgg
     4981 cgccgccaac cgctgcctcg attgcgctca gcggtgtcgt gtccacggcg cagatgctac
     5041 cgccgacctc cgacagcggg cagcggtccg gtgcccggcg gcgctgttct gcgcgcggtt
     5101 ctgcgcgctg ttccgctttg ccgttccgag gtgacttgca gcgcattgaa acatgagtga
     5161 agaatcacgt ctcaaagatg acggaatggt catcttccct ggaccgcccg acctcggaac
     5221 gatccgaaac ccacctcatc gtcaccacca aggggattgc cgccacatga ccacgatcga
     5281 ccttcccggc cttcaacgtt cgacctccgt tctcgatcag attcgagacg gcgtctcgtt
     5341 cggcctgctc ttcggcggcc agggcggcga ctaccggcct cgagtcgagg cgttgatcgg
     5401 aactcaccga accgagatcg ccgcgccctc gctgtggggt gacgcattgg ctcgcctcga
     5461 tgctcgactc gacacgatgt tggcgtggta tccccgcgat ccccgatccg cacatctgcc
     5521 cgacccgcct cgacccacca caccccgcct ccatcccgaa cgctccgatc ccgaacgagc
     5581 ccaccccgcg cgatcccatc cggcacgatc ccatccggca cgatcccatc cggcactgtc
     5641 ggtgccggga atcgcgttcg cccaactcct cgaacggcgc gccctacgcc tgcacggcat
     5701 cggagggagc ggccggcacg acaccgaacc cgccccaagc gtcgcgatcg gtcacagcca
     5761 ggga
//
LOCUS       DAOOMA010000091         5754 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_16748_length_5754_cov_4.927180, whole genome shotgun sequence.
ACCESSION   DAOOMA010000091 DAOOMA010000000
VERSION     DAOOMA010000091.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 5754)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5754)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5754
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16748_length_5754_cov_4.927180"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1478)
                     /locus_tag="PLP95_03850"
     CDS             complement(<1..1478)
                     /locus_tag="PLP95_03850"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HPB44965.1"
                     /translation="MTAVGTLLLELSESDRPALLYGDRSRSYRELVAEGRRRAAWFES
                     VRDPQRPPHIGVLLDNVPDYLFWLTGAALSGSVIVGINATYRGEQLGQLVEYTDCQAI
                     VTSSNLVGLLDDVDTAVAPEMRLVVDAEDYDQRLDAAAEVTVEYQPADDDLFMLIFTS
                     GSTGLPKAVRCTQGRFGRTGAHVANIASLGDDDVAYAPLPFFHAASMFTGWASAINAA
                     IPIATRDKFSRSATVGDLRKFGATYMTYTGKVLNYILSMPEDPTDTGLGLRLAIGNEA
                     SEHDIREFARRFNCQVRDSYGSTEGVIIVRRDETMPPGALGTANESVKVVDPETGVEC
                     PRAHVGADGQITNLDEAIGELVETAPASGFEGYYKNEAATAERFRGGWYWSGDLAYRD
                     AEGWFFFAGRTNDWLRVDGENFSAGPVEKILARFPGVRSVAVYAVPDDPVGDRVMAAL
                     EMTGQVDDGLGNAAFDPAAFDLAAFEAFLSEQPDLGPKWVPS"
     gene            complement(1475..2485)
                     /locus_tag="PLP95_03855"
     CDS             complement(1475..2485)
                     /locus_tag="PLP95_03855"
                     /inference="COORDINATES: protein motif:HMM:TIGR03559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HPB44966.1"
                     /translation="MELGLSTHGLSLAPSDLAELGRIAEDSGYDSIWTAEAWGADAFT
                     PLAFLAATTTRLKLGTAIAQVWARTPGATAMTALTLQQLSEGRLVLGLGVSGPQVVEG
                     WHGVAFRRPLAVTRDYVAIVRQALAADTKVSYEGSELSVPYRGAEATGQGRPLRSTQP
                     GWPDTPIYLAALGPNNVRLAGEVADGLLPYLWSAARWEAAWGEVLGAARPGFAVAPTV
                     VAALGDDLAACRDQVRPRIALHVGAMGSKQTNFYKSLVTRYGYGEEAERIQDLWLSGD
                     RAGSIAAVTDEMVDDMTLVGPAGHIDEQLQRWRSGPITTLIVEPTTPAAIAPLAEIWR
                     GL"
     gene            complement(2535..3542)
                     /locus_tag="PLP95_03860"
     CDS             complement(2535..3542)
                     /locus_tag="PLP95_03860"
                     /inference="COORDINATES: protein motif:HMM:NF012656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HPB44967.1"
                     /translation="MTSANEPDDEFLAAATRALHTTPGPEALDELGWWDLLGDGIALW
                     GDPDAREAVFAVFRAQGRELADTAALGALVASTFLVAAGRGPGEAVAVLPRGASKGSG
                     VDTWTLIGPVGRRPLLVDRAGSGVWLVDLAEVAVTAVELSGRFSLAEVSIPPDAGELI
                     LTEPRAAAVRDRARYLARLALATELHGLAERIVDLAVEYATVREQFSKPIGTFQAVRH
                     LLSWASTDVTAIGSVNRRGLDFADSPPARFDEVVKALAGRNARQACQRSLQVFGGIGF
                     TAEHPHHHYQSRVLMLDALLGSSAELTHGLGRWLREVNTDPGFTAAAAHLAAATSSGS
                     R"
     gene            complement(3539..4720)
                     /locus_tag="PLP95_03865"
     CDS             complement(3539..4720)
                     /locus_tag="PLP95_03865"
                     /inference="COORDINATES: protein motif:HMM:NF014790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HPB44968.1"
                     /translation="MGDAVIVSTARTPIASADEGSLADIADYSAAFEAWLSTECATLA
                     AVCEPIVDYGQRVEAMRAFMAELFEAGWARYGWPVALGGLGGTIAHRAVMWEVAARFG
                     VQGMALFEHLEILAPTLAVHGPSELIADLLPRFLSGRELWAQGFSEPNAGSDLANLRT
                     TAVLDGDSYRITGQKIWTSWARYATWCLVLARTGEPGSAHRGLSAFIVDLRSPGVDVR
                     AIEQANGTDELAEVFFDEVVVPADRIVGTVGGGWAVAMHILSNERGTFAWFRHCFLYQ
                     QLLDAAGRATARDDVAFGDALLDLAAVTATGYAGVRAHGLGHQLGVKAAYTKLLLCAS
                     EQSVNDAVLASDEGIAIGPFDAQTALRRQEYLFSRIVTVYGGSQQMQLDTIAKQILRL
                     P"
     gene            4846..>5754
                     /locus_tag="PLP95_03870"
     CDS             4846..>5754
                     /locus_tag="PLP95_03870"
                     /inference="COORDINATES: protein motif:HMM:NF013920.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold domain-containing protein"
                     /protein_id="HPB44969.1"
                     /translation="MRLLPELTPVNEWFWTSGADGVLRMQRCEDCTRIAHPPTPICPE
                     CRSRNRGIVEVSGRATVVGVTVNHHPWLPGFDPPYVIANVALAEDPTVRLTTNIVDCD
                     PDTVRIGDVVEVRFEEYGHKGSAPLHLPLFTPTGERDDRDLVGEPAIATPRAPLGSER
                     FEHSAVLSGVGRSDIGRRLMRDPLSLTVDAALAAIADAGLEIDDIDGLSTYPGPLGMG
                     MSEGGVEAVTEALRLNPTWHNGGMELPGPGGSIIAAVQAVANGLCRHVLCFRTVWETT
                     HSALGLSRGGGLTAQEEDAALAAGFVP"
BASE COUNT          985 a         2043 c         1836 g          890 t
ORIGIN      
        1 aaggacggca cccacttcgg gcccaggtcg ggctgctcgc tcaggaacgc ctcgaaggcc
       61 gctagatcga aagcagcagg atcgaaggct gcgttgccca acccatcgtc gacctgcccg
      121 gtcatctcaa gcgccgccat cacccggtca ccgaccggat cgtcgggcac cgcgtacacc
      181 gctacggaac gcacgcctgg gaatcgcgcc aggatcttct cgaccggccc tgcggagaag
      241 ttctccccgt cgacccgcaa ccagtcgttg gtgcgcccgg cgaagaagaa ccaaccctcc
      301 gcgtcgcgat aggccaggtc ccccgaccag taccacccgc ctcggaaccg ctcggccgtt
      361 gcggcctcgt ttttgtagta cccctcgaac cccgatgccg gagcggtctc gaccagctca
      421 ccgatcgcct cgtcgagatt ggtgatctga ccgtccgcac cgacatgcgc acgagggcat
      481 tcgacccccg tttcggggtc gaccaccttc accgactcgt tcgccgtgcc gagcgcgccg
      541 ggcggcatcg tctcgtcgcg tcgcacgatg atgacccctt cggtcgagcc gtagctgtcg
      601 cgcacctgac agttgaaacg tcgagcgaac tcgcggatgt cgtgttcgga ggcctcgttg
      661 ccgatagcga gccgtaaccc caacccggta tcggtcgggt cttccggcat cgacaagatg
      721 tagttcagca ccttgccggt gtaggtcatg taggtggcgc cgaacttgcg caggtcgccg
      781 acggtggcag accttgagaa cttgtcgcgg gtggcgatgg gaatcgccgc gttgatcgcc
      841 gacgcccagc ccgtgaacat cgacgccgcg tggaaaaacg gcagcggcgc gtacgcgacg
      901 tcgtcgtcgc ccaacgaggc gatgttcgcc acatgggcac cggtgcgacc gaagcgtccc
      961 tgtgtacagc gcaccgcctt gggtagcccc gtcgacccgg aggtgaagat caacatgaac
     1021 agatcgtcgt cggcaggctg gtactcgacg gtcacctccg ccgcagcgtc gaggcgttgg
     1081 tcgtagtcct cggcatcgac gacgagtcgc atctcgggtg cgacggcggt gtcgacatcg
     1141 tcaagaagac cgaccaggtt ggaggaggtg acgatcgcct gacaatcggt gtactcgacg
     1201 agttgtccga gttgttcgcc gcgataggtg gcgttgattc cgacgatcac cgagccggac
     1261 agggcggcgc cggtcaacca gaacaggtag tcaggaacgt tgtccaacaa cacgccgata
     1321 tgcgggggcc gctgcgggtc gcgaaccgac tcgaaccagg cggcccggcg acgcccctcg
     1381 gccacgagtt cgcggtacga ccgcgaccgg tcgccgtaca gcaacgccgg ccggtcgctc
     1441 tcgctcagct ccaacagcaa cgttccgacg gcggtcacag tcccctccaa atttcagcca
     1501 atggcgcgat cgccgcgggt gtcgtgggct cgacgatcag cgtggtgatc ggcccgcttc
     1561 gccaacgttg aagttgctcg tcgatgtggc cggcagggcc gacgagcgtc atgtcgtcga
     1621 ccatctcatc ggtgaccgcg gcgatcgacc cggcacggtc acccgacagc cacaggtcct
     1681 gaatgcgctc cgcttcctcg ccgtagccgt agcgggtcac gagagacttg tagaagttcg
     1741 tctgcttcga acccatcgca ccgacgtgca gggcgatgcg tggacgcacc tgatcgcgac
     1801 acgccgcgag atcgtcgccg agcgcggcca ccaccgtggg tgcgacggcg aagcccgggc
     1861 gggccgcgcc gagcacctcg ccccacgccg cctcccaacg ggccgcactc cacaggtacg
     1921 gcaacaatcc gtcagcaacc tcgccagcga ggcgcacgtt gttgggcccg agcgccgcta
     1981 ggtagatcgg cgtgtcgggc cagccgggtt gggtcgaacg aagcgggcgg ccttgaccgg
     2041 tcgcttcggc accccgatac ggaacgctca gctccgagcc ttcgtagctc actttggtat
     2101 cggccgcgag cgcctgacgc acgatcgcca cgtagtcgcg cgtcaccgcc aacgggcgcc
     2161 gaaacgccac gccgtgccat ccctcgacca cctgtggccc cgacaccccg agccccagca
     2221 ccaggcgacc ctcggacagc tgctgcaggg tcagcgcggt catcgctgtt gccccgggcg
     2281 tgcgcgccca cacctgagcg atagcggtgc cgagtttcag ccgggtcgtc gtcgcggcga
     2341 ggaacgcgag cggggtgaag gcgtcggctc cccacgcctc tgcggtccag atcgagtcgt
     2401 agcccgagtc ctccgcgatc cgcccgagct ccgccaagtc actcggagcc aaactcaacc
     2461 cgtgggtgct caacccaagt tccacgatga tctcccctca ctggcgccag caggaaaccg
     2521 acgaacggac cggtctagcg cgaccctgac gacgtcgccg ctgcgaggtg cgccgctgcg
     2581 gcagtgaatc ccggatcggt gttcacctcg cgcagccaac gtcccaaccc atgggtcagc
     2641 tcggccgagg agcccagcag cgcatcgagc atcagcacac gactctggta gtggtgatga
     2701 ggatgctcgg cggtgaaccc gatccctccg aacacctgca gggaacgctg acacgcctga
     2761 cgggcgttgc gccccgccag cgccttgacc acctcgtcga aacgcgccgg tggcgagtcg
     2821 gcgaaatcca gtccacggcg attcacgctg ccgatggcgg tgacgtcggt cgatgcccac
     2881 gacagcagat gtcgcactgc ctgaaacgtt ccgatcggct tcgagaactg ctcgcggacg
     2941 gtggcgtatt cgaccgcgag gtccacgatc cgttccgcca gcccgtgtag ttccgtcgcc
     3001 agtgcgagcc tggcgagata cctcgcacga tcccgcacgg ccgccgctcg cggttcggtg
     3061 agaatcagct cacccgcgtc gggcgggatc gagacctcgg cgaggctgaa acgccccgac
     3121 aactcgaccg cagtgacggc tacttcggcc aggtcgacga gccagactcc cgatcccgca
     3181 cgatcgacga gcaacggccg ccggcccacg ggtcctatga gggtccacgt gtcgaccccg
     3241 ctgcctttcg acgcgcctcg cgggagcact gcgacggcct cgcccggccc tcgaccggcc
     3301 gcgacgagaa aggtcgacgc gaccaacgcc ccgagggcgg cggtgtcggc gagttcacgc
     3361 ccctgggcgc gaaagaccgc aaagaccgcc tcgcgcgcgt ctgggtcgcc ccacagagcg
     3421 atcccgtcgc cgagcagatc ccaccacccg agttcgtcga gcgcttcggg ccccggcgtg
     3481 gtgtgcaacg cgcgggtcgc agcggcgagg aactcgtcgt cgggttcgtt cgccgacgtc
     3541 atgggagcct cagaatctgt ttggcgatcg tgtcgagctg catctgttgg gaccctccgt
     3601 acaccgtcac gatccgcgag aacaggtact cctggcggcg caacgcggtc tgtgcatcga
     3661 aggggccgat ggcgatgccc tcgtcgctcg ccagcaccgc gtcgttcacc gattgctccg
     3721 aggcgcacaa caacagcttg gtgtacgccg ctttgacgcc gagttggtgt ccgagcccgt
     3781 gtgcccgaac cccggcgtac ccggtggcgg tgaccgcagc taggtcgagt agcgcatctc
     3841 cgaaggcgac gtcgtcacgc gccgtcgccc gacctgcagc atccagcagc tgttggtaca
     3901 agaaacaatg acggaaccag gcgaaggtgc cgcgctcgtt gctcaagatg tgcatggcga
     3961 ccgcccagcc gccgccgacc gtcccgacga tgcgatcggc gggcacgacg acctcgtcga
     4021 agaacacctc ggcgagttcg tcggttccgt tcgcctgctc gatcgcccgc acgtcaaccc
     4081 cgggtgaacg gagatcgacg atgaacgcgc tcagtccgcg gtgcgccgat ccaggctcgc
     4141 cggttcgggc cagcaccaga caccacgtcg cgtaccgcgc ccaactcgtc cagatcttct
     4201 ggccggtgat ccgatacgag tcgccgtcga gcaccgctgt cgtgcggagg ttggcgaggt
     4261 ccgacccggc gttgggttct gagaacccct gtgcccacag ctcccgaccc gacaagaacc
     4321 tcgggagcag atcggcgatg agttcgctcg gaccgtgaac agcgagggtc ggggcgagga
     4381 tctcgaggtg ctcgaacaac gccatgccct gcaccccgaa tcgggccgcg acctcccaca
     4441 tgaccgcccg atgcgcaatc gttccgccca acccgcccag tgcgaccggc cagccgtacc
     4501 gggcccatcc ggcctcgaac agttcggcca tgaaggcccg catcgcctcg acccgttgcc
     4561 cgtagtcgac gatcggctcg cacacggccg ccaaggtcgc acactcggtg ctcaaccacg
     4621 cttcgaaggc cgcgctgtag tccgcgatgt cggcgagcga accctcgtcg gccgatgcga
     4681 tcggcgtgcg tgcggtggaa acgatgacgg cgtcgcccat cggtggctcc tcggattcgc
     4741 gcgtgaggtg gaggttacga agatgttgac atcgacgtca acatcgaacc agtggcgctc
     4801 cgcccgatac gatcgccacg gatctgcatc gaaaggaccc gtgcggtgcg cctgctgccc
     4861 gaactcaccc ccgtcaacga atggttttgg acctccggcg ccgacggcgt cctgcgaatg
     4921 cagcgttgtg aggactgcac ccggatcgct catcccccga cccccatctg ccccgagtgc
     4981 cggagccgca accggggcat cgtcgaggtc tcggggcgcg ccaccgtcgt cggagtcacg
     5041 gtcaatcacc atccgtggct tccgggtttc gacccgccct atgtcatcgc gaacgtcgcc
     5101 ctcgccgagg atccgaccgt gcggctcacg accaacatcg tcgactgcga ccccgacacc
     5161 gttcgcatcg gcgacgtcgt cgaagtccgc ttcgaggaat acgggcacaa gggttccgct
     5221 ccgctccacc tgccgctgtt caccccgacc ggggagcgcg acgaccgcga cctcgtgggc
     5281 gaaccggcca tcgcgacacc tcgggcgccg ctcggctccg agcgattcga acacagcgca
     5341 gtgctgtcgg gggtcggccg gtccgatatc ggccgacggc tcatgcgcga cccgctgtcg
     5401 ctcaccgtcg acgccgccct cgcagcgatc gcggatgccg gacttgagat cgacgacatc
     5461 gacggcctgt cgacctaccc cggcccgctc ggcatgggga tgagcgaagg cggcgtcgaa
     5521 gctgtgaccg aggccttgcg cctcaacccc acctggcaca acggtggcat ggaactaccc
     5581 ggccccggcg gcagcatcat cgccgccgtg caggccgtcg ccaacgggtt gtgtcgtcac
     5641 gtcctgtgtt ttcgcacggt ctgggagacg acgcactcgg cgctcgggct cagccgcggc
     5701 ggcgggctga cggcgcagga ggaagacgcg gcgctggccg caggctttgt cccc
//
LOCUS       DAOOMA010000092         5723 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_16861_length_5723_cov_4.786697, whole genome shotgun sequence.
ACCESSION   DAOOMA010000092 DAOOMA010000000
VERSION     DAOOMA010000092.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 5723)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5723)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5723
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16861_length_5723_cov_4.786697"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(198..1217)
                     /locus_tag="PLP95_03875"
     CDS             complement(198..1217)
                     /locus_tag="PLP95_03875"
                     /inference="COORDINATES: protein motif:HMM:NF018028.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HPB44970.1"
                     /translation="MTAIGERPDGSDVTLLRTGRIGRRLVAFGIDATVLAYCIALGLL
                     GIAFIDAIMTSTLGGCATSDAPADCEPSIWIARFIAALAAMWIAASVHLVLVVPAWRN
                     GATLGMRQVGIRIVSHHYGAVVSQRQFHLRAWVSHSSAIVLILVLFSWVIGPAGWLDR
                     VIQGVAIAGGVVIVASAVLILLPGHRSIADSLTATRVIVERPVSYIAHIAAIVCSVIG
                     QALLSVATAWTFGRPDEPDLSFDFGNRRHDDFDWASDPLERLFAWSDSATSNTIALVV
                     CGAIWLAAVGLCWAGLVDTRWHAERNAGRGVALVACLYAALPILVLTIWAVTRVASIL
                     IDAFG"
     gene            1336..1740
                     /gene="mce"
                     /locus_tag="PLP95_03880"
     CDS             1336..1740
                     /gene="mce"
                     /locus_tag="PLP95_03880"
                     /EC_number="5.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012784194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA epimerase"
                     /protein_id="HPB44971.1"
                     /translation="MILTEIDHVAIAVNDLEAAIEYYRSAFGAEVHHREIVDSDGVEE
                     ALVKVADSYIQLTAATRPDSPIAKSIEKRGEGLHHIGYRVDDCQAALDAMVAAGATPI
                     DKAPRPGSRGTTVAFIHPKGSFGTLIELVQEN"
     gene            1836..2720
                     /locus_tag="PLP95_03885"
     CDS             1836..2720
                     /locus_tag="PLP95_03885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012226157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-3' exonuclease H3TH domain-containing
                     protein"
                     /protein_id="HPB44972.1"
                     /translation="MQVHLVDGTYELFRYFYAPGGGKHLNDVGEEVGAVRSVLRSVTA
                     LLEDGATHVGVATDHVIESFRNELYAGYKDGSGIDPVLFGQFPLLEAALEAAGFTVWA
                     MVEFEADDALGAAAAVAAADPDVTRALICTPDKDLAQCVVDPKVAQFDRRAGVIRDEA
                     AVLEKFGVMPASIPDWLGLVGDTADGFPGLAGWGAKSAAAVLRRYEHIENIPLAPGQW
                     DITVRGSQKLCSVLAEHLDDALLFRTIATIRLDVPVGTPAQWRWSGVGSGFDEMCDRL
                     DAHDVHRRLTKLAGQRFG"
     gene            complement(2754..3812)
                     /locus_tag="PLP95_03890"
     CDS             complement(2754..3812)
                     /locus_tag="PLP95_03890"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003910 - DNA ligase (ATP) activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA ligase"
                     /protein_id="HPB44973.1"
                     /translation="MLSKAATSLPDGEDHGFVFEPKWDGFRCIVFRDGDDVLLGSRND
                     KPLTRYFPELMDPIREMLPERIVVDGEVVVATGRGLDFDALGQRIHPAESRIAKLSVE
                     TPASFVAFDLVALGDTDHQTTPFGERRALLESVMDPTSPPLHLSPATTDRTVAADWFT
                     RFEGAGFDGVMAKATGDPYLQNKRSILKIKHQRTADVVVAGYRVHKDGAGVGSLLLGL
                     LDDDATLHSIGVASSFAAARRVELLDELAPFVDGGLDGHPWAEWGEAEAHATSGRMPG
                     APNRWNQQRDQSWVPLRCELVAEIGYQGLTAGRLRHPAKFLRWRPDKAPDQCRYDQLD
                     APVPIELAHLFSNGEFDR"
     gene            complement(3842..4441)
                     /locus_tag="PLP95_03895"
     CDS             complement(3842..4441)
                     /locus_tag="PLP95_03895"
                     /inference="COORDINATES: protein motif:HMM:TIGR00730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HPB44974.1"
                     /translation="MTSTNKTSRPQLCAYLASRPGGDPAFSDMARRTGRAVAESGADL
                     VYGGGSSGLMGDIADACLATGGRVTGVITEHLYDLEVAHQAVTELIVVGDMPERKREM
                     FERADGFLVLPGGIGTMEELFEVWCWAALGLHPKALGLLNVNGFYDRLLEFMQRAASD
                     GLMAPSTLELLFVDDDPQRLVERVLNAVAAHHPSGTMSL"
     gene            4500..>5723
                     /locus_tag="PLP95_03900"
     CDS             4500..>5723
                     /locus_tag="PLP95_03900"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012224862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA carboxylase subunit beta"
                     /protein_id="HPB44975.1"
                     /translation="MDHSLAERVDDLLRRKEEARQPGSERSVERQRSRGKMLARERIE
                     YLLDEGSFHELDGLARSRSASATERPYTDGVVTGWGTVEGRQVFVFSQDFTLFGGALG
                     EVYAEKIHKVMDMALKVGAPVIGLNDGAGARIQEGSVSLASYGGIFYRNVKASGVTPQ
                     ISVIMGPCAGGSVYSPILTDFIFMVDETSYMFITGPDVVKQVTGEDVSQQDLGGARVH
                     SSKSGVAQFVSADDKGALDDVRFLLGFLPSNNLEEAPRVDTDDPHDRLVPELIDMIPA
                     SANQAYDMRSVVEAIVDDNEFFEYAPAWAPSIICGFARLGGQSVGIVGNQPMAFAGVL
                     DIESSSKAAKFVRTCDAFNIPLVTFVDVPGFLPGVEQEHNGIIRHGAKLLYAYCEATV
                     PRISVITRKAYGGAYV"
BASE COUNT         1022 a         1851 c         1883 g          967 t
ORIGIN      
        1 caggtcggcg aggccgccct taaagtccac cgcaaccagg ccgaaggcaa actgggtgtg
       61 ctgtgcctgg ctccggaggc cggccagggc atcaccgacc cggagttccg cgagcaggtc
      121 ggtgaggatc gcatcacgtt gtttgcccgc cacgcccgcg gcgagctgta acgaccggcg
      181 gccaggagcc gccggtttca accgaatgcg tcgatgagga tcgacgcgac cctcgtgacc
      241 gcccagatcg tcaacacgag gatcggaagt gcggcataca agcacgccac gagcgcaacg
      301 ccacggccgg cgttgcgctc ggcgtgccag cgggtgtcga cgaggcccgc ccaacaaagg
      361 ccgacggcgg ccaaccagat ggccccgcac acgacgaggg cgatggtgtt cgaggtggcg
      421 gaatcggacc aggcgaaaag tcgctcgagc ggatcgctcg cccagtcgaa atcgtcgtgg
      481 cgtcgattgc cgaagtcgaa cgacaggtcg ggctcatcgg ggcgaccgaa tgtccaggct
      541 gtcgcgaccg acagcagggc ttggccaatg accgaacaga cgatggcggc gatatgggcg
      601 atgtaggaca cgggtcgttc cacgatgacc cgtgtggccg tgagcgaatc ggcgatactg
      661 cggtgcccgg gcaacaagat gagcacggcc gaggccacga tcacgacacc accggcgatg
      721 gccacccctt ggatcacccg gtcgagccac ccagccgggc cgatcaccca tgagaacagc
      781 acgaggatca acacgatcgc cgaggaatgg gagacccagg cccgtaggtg aaactgccgc
      841 tggctgacga ccgcaccgta gtgatgcgac acgatgcgta tcccgacctg gcgcatcccg
      901 agggtcgcac cattgcgcca cgctggcacg acgagcacca ggtgcaccga cgccgcgatc
      961 cacatcgccg cgagagctgc gatgaaccgc gcgatccaga tcgacggttc acagtcggcg
     1021 ggcgcgtctg acgtcgcgca gcctcccagt gtcgaggtca tgattgcgtc gatgaacgcg
     1081 atgcccagaa gtcccaacgc gatgcagtaa gcgagaacgg ttgcatcgat tccgaaggcg
     1141 accaggcgtc gtccgatccg tccggtgcgc agcaacgtga cgtctgaccc gtctggccgc
     1201 tcgccgatcg cggtcatcgt ttccatggtt cggggaccct cctgtcctcg gtcggatcgt
     1261 acccactttg gcgccgatga ccaggactga gttctcgcat ggtcgctgcg gcatcggtcg
     1321 tggaacactg gaggcatgat tctgaccgag attgatcacg tggccattgc cgtcaacgac
     1381 ctcgaggcgg cgatcgagta ctaccgcagc gcattcggcg ccgaggtgca tcaccgcgag
     1441 atcgtcgact ccgacggtgt cgaggaggcc ctggtcaaag tggccgattc gtacatccag
     1501 ctcaccgcgg caacccgccc ggattcgccc atcgccaagt cgatcgaaaa acgcggcgaa
     1561 ggcctccacc acatcgggta ccgcgtggac gactgccagg ccgcgctcga cgcgatggtc
     1621 gccgcgggcg cgaccccgat cgacaaggcc ccacgccccg gcagccgtgg caccacggtg
     1681 gcgttcatcc acccgaaggg cagcttcggg acgctcatcg aactcgtgca ggaaaactga
     1741 tcgtcggcct ctcttgaggg cttgcggtcg acgccttggc gtgcggcgtc cgagccgttc
     1801 gagttgtcac cgtcgagttg tggcagggtt cttccatgca ggtccacctt gtcgatggca
     1861 cgtatgagct ctttcggtac ttctatgcgc cgggtggcgg caagcacctg aacgacgtcg
     1921 gtgaagaggt tggtgcggtt cgcagcgtgc tgcgctcggt caccgcgttg ctggaggacg
     1981 gggccaccca cgtcggggtg gccaccgacc atgtgatcga gagtttccgc aacgagctct
     2041 atgccggcta caaggacgga tcgggaatcg acccggtgct gttcgggcaa ttcccgctcc
     2101 tcgaggcggc gctggaggcg gccgggttca cggtctgggc aatggtggag ttcgaggccg
     2161 acgacgcgct cggcgcggcg gccgcggtcg ccgcggcgga ccccgacgtc acccgcgcgt
     2221 tgatctgcac ccccgacaag gatctggcgc aatgcgtcgt cgacccgaag gtggcgcagt
     2281 tcgatcgccg ggcaggcgtt atccgcgacg aggcggcagt gttggagaaa ttcggggtga
     2341 tgccagcgtc gattccggat tggctcgggc tggtcggcga caccgcagac ggctttcccg
     2401 gtctcgccgg ttggggcgcg aagtcggcag cggcggtgtt gcgacgctac gagcacatcg
     2461 agaacattcc cctcgccccg gggcagtggg acatcaccgt tcgcggatca cagaagctgt
     2521 gctccgtact cgccgagcat ctcgacgacg cactgctgtt ccgaacgatc gccacgatcc
     2581 ggctggatgt tcctgtcgga actccggcgc aatggcgttg gagcggggtc ggttccgggt
     2641 tcgacgaaat gtgcgatcgt ctcgacgccc acgacgtgca ccgccgactc acgaagctcg
     2701 ccgggcagcg cttcggctga ggcgggttcg cgacctgggg caacggctgg gggtcaacga
     2761 tcgaactctc cgttggagaa caggtgcgcc aactcgatcg gcaccggcgc gtcgagttgg
     2821 tcgtaacggc actgatcggg cgccttgtcg ggtcgccaac gaaggaactt cgccggatgt
     2881 cgaagccgac cggcggtaag accctgataa ccgatctccg cgacgagttc gcagcgaagc
     2941 ggcacccatg attggtcgcg ctgttggttc caacggttgg gtgcgcccgg catgcgtcca
     3001 gacgtcgcat gcgcctcggc ctcgccccat tcggcccatg ggtggccatc gagtccgccg
     3061 tcgacgaatg gggccaactc gtcgagcagt tccacccgcc gcgccgcggc aaagcttgag
     3121 gccacaccga tggaatgcaa ggtcgcgtcg tcatcgagga gacccaacag cagcgacccg
     3181 acgccggcac cgtccttgtg aacccgatag ccggccacca ccacgtctgc ggtgcgctgg
     3241 tgcttgatct tgaggatcga gcgcttgttt tgcaggtacg gatcgccggt ggccttggcc
     3301 atcactccgt cgaatccggc gccctcgaat cgggtgaacc agtcggcggc cacggtgcgg
     3361 tcggtggtcg ccggcgacag gtgcagcggc ggcgacgtcg ggtccatcac cgactccagc
     3421 agcgctcggc gctcgccgaa cggtgtcgtc tggtggtcgg tgtcgccgag ggcgacgaga
     3481 tcgaaggcga cgaagctcgc cggcgtctcg accgagagct tggcgattct cgattcggcc
     3541 ggatggattc gttggccgag cgcgtcgaaa tcgagcccac gtccggtggc gaccaccacc
     3601 tcgccgtcga ccacgattcg ctcgggaagc atctcgcgta tcggatccat caactcgggg
     3661 aagtaccgag tcagcggctt gtcgttgcgg ctaccgagca gaacgtcgtc gccgtcacga
     3721 aagacgatgc agcgaaaccc atcccatttc ggctcgaaca cgaagccgtg gtcctcgccg
     3781 tcgggaagcg acgttgctgc ctttgacagc atgggtcgaa aaggggggac aaccggcaac
     3841 gtcatagcga catcgtgccc gatggatggt gagcagcgac ggcgttcagt acgcgctcca
     3901 ccagccgttg gggatcgtcg tcgacgaaga gcaactcgag ggtggagggc gccatgagtc
     3961 cgtcggacgc cgcgcgctgc atgaactcga gcagccggtc atagaagccg ttcacgttca
     4021 acaacccgag ggccttgggg tgcagtccga gcgccgccca gcaccacacc tcgaaaagct
     4081 cctccatcgt cccgatgccg ccgggaagga cgagaaaccc gtcggcgcgc tcgaacatct
     4141 cccgtttccg ttcgggcata tcgccgacga cgatcaattc ggtcacggct tgatgcgcaa
     4201 cctcgaggtc gtagaggtgt tcggtgatga ccccggtgac gcgaccgccg gtggcgaggc
     4261 acgcgtccgc gatgtcgccc atcaacccgg agctgccgcc gccgtagacg agatcggctc
     4321 cggactcggc caccgctcga ccggtgcgtc gggccatgtc ggaaaacgcc gggtccccgc
     4381 cggggcgcga cgctaggtag gcgcagagtt gaggacgtga cgtcttgttg gtcgaggtca
     4441 tgagtcgcca cggtagcgat tcggtgcggt ttggcgtttc gccgtaatct ctcaaaccta
     4501 tggatcactc gcttgccgaa cgcgtcgacg acctcctccg ccgcaaggaa gaggcccgcc
     4561 aacccggttc cgaacgctcg gtcgagcgtc aacgttcccg gggaaagatg ctcgctcggg
     4621 aacggatcga gtacctcctc gacgaggggt cgttccacga actcgatggt cttgcccgct
     4681 cacgctcggc ctctgcaacc gaacgcccct acaccgacgg tgtggtgacc ggttggggaa
     4741 cggtcgaggg gcgacaggtc tttgtcttct cacaggactt cacgctgttc ggcggagcgc
     4801 tcggtgaggt ctacgccgaa aagatccaca aggtcatgga catggcgttg aaggtcggag
     4861 cccccgtcat cgggctgaat gacggcgccg gcgcccgtat tcaggaaggt tcggtgtcgc
     4921 tcgccagtta cggcggcatc ttctatcgca acgtgaaggc ctcgggggtc accccgcaga
     4981 tctcggtgat catgggccca tgcgcgggcg gctcggtgta cagcccgatc ctgaccgact
     5041 tcatcttcat ggtcgacgag acgtcgtaca tgttcatcac gggtcccgac gtcgtgaagc
     5101 aggtcaccgg cgaggacgtg agccaacagg acctcggtgg cgctcgggtt cacagctcga
     5161 agtccggtgt cgctcagttc gtttccgccg atgacaaggg cgccctcgac gacgttcggt
     5221 tcctgctcgg cttcttgccc tccaacaacc tggaggaagc accccgggtc gacaccgacg
     5281 acccccacga ccgactcgtg cccgagctca tcgacatgat ccccgcctcg gcaaaccagg
     5341 cctacgacat gcgttcggtg gtggaggcga tcgtcgatga caacgagttt ttcgagtacg
     5401 cgccggcgtg ggccccttcc atcatctgcg gctttgcccg ccttggcggc cagtcggttg
     5461 gcatcgtcgg caatcagccg atggcgttcg ccggggtgct cgacatcgag tcctcctcga
     5521 aggcggcgaa attcgttcgc acctgcgacg ccttcaatat tcccctcgtg accttcgtcg
     5581 atgtccccgg cttcctgccc ggcgtcgagc aggaacacaa cggcatcatc cgccacggcg
     5641 ccaagctgct gtacgcctat tgcgaggcaa ccgtgccccg tatctcggtg atcacccgca
     5701 aggcctatgg cggcgcgtac gtc
//
LOCUS       DAOOMA010000093         5672 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_17075_length_5672_cov_3.393448, whole genome shotgun sequence.
ACCESSION   DAOOMA010000093 DAOOMA010000000
VERSION     DAOOMA010000093.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 5672)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5672)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5672
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17075_length_5672_cov_3.393448"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..191
                     /locus_tag="PLP95_03905"
     CDS             <1..191
                     /locus_tag="PLP95_03905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB44976.1"
                     /translation="YELDRWRDQQAAIYGYRTVRFTHRQVTTQPDYVRATMAALLDAP
                     TFGTLATQPAPPTSPAHS"
     gene            complement(336..1211)
                     /locus_tag="PLP95_03910"
     CDS             complement(336..1211)
                     /locus_tag="PLP95_03910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA ester lyase"
                     /protein_id="HPB44977.1"
                     /translation="MTEVIVPRRSVLYMPGANDKALEKAKGLATDAIIFDTEDSVAPD
                     MKEVARAKVADAVRSGEYGKRELTIRVNGLDTQWFEDDLRSAGAAGPHGVVVPKVNSA
                     ADVATIERLLESVAGAENTRIWAMLETPTAVENAVEIATASERLAVLVMGTNDLAKEL
                     RAGLVPGRHPLLWGLGRCVNAARYAGKVILDGVYNDVRNPEGFAIEAQQGAEMGFDGK
                     TLVHPSQVEPCNEAFAPSEDEIDYSRRVIEAFEAGIAEGKGVVTVDGKMIENLHVDNA
                     RRALAIAEAIAALNS"
     gene            complement(1225..2286)
                     /locus_tag="PLP95_03915"
     CDS             complement(1225..2286)
                     /locus_tag="PLP95_03915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA ester lyase"
                     /protein_id="HPB44978.1"
                     /translation="MRSAKDFFKPLAAGAPQPVTEIPFPPSRMIHFFPPHLDKVTAKL
                     PEMATKVDILLGNLEDAIPADQKENARAGLVKVAKEMDLGDAQLWTRVNSLDSPWVLD
                     DLITLVTEVGDRLDVIMIPKVEGPEDIHYVDRLLAQLEARAELTKPLLLHAILETARG
                     VANVEEIALASPRMQGISLGPADLAASRRMKTTRVGGGHPGYLVRQDPPRGEDGPVYD
                     AERSSYQQDLWHYTISRMVDACAMAGILPFYGPFGDIKDTVACEDQFRNAFLLGCVGA
                     WSLHPVQVDIAKKVFSPSKEDIDHALHVIEEMGDGTGAVMIDGKMEDDASVKQCHVMV
                     NLAKMLAARDPELAELYGF"
     gene            complement(2385..3875)
                     /locus_tag="PLP95_03920"
     CDS             complement(2385..3875)
                     /locus_tag="PLP95_03920"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HPB44979.1"
                     /translation="MGATSASTPSPSGFASASRRAGAVRGPSMVDHALADASPTVLWL
                     DREDRPEAGPALGQTGNGADDAVDLVIVGGGYSGLWAAIQSMQDDPNRSVVVIQSGRI
                     AEQASGRNGGFCSSSLTHGLDNGASRFGEDLKRIEAEGRASFAGIRDTIEHHGIDCDW
                     HEPGQLTVATAPWLVDDLHASAELSRKHGYDIDVLDAEATRSELDSPTYHAGLWQRDG
                     EALVDPARLGWGLAAAARSLGVRIHERTTMVGLERHGAGMAVRTDRGALRCGAVILAT
                     NAFRSPLRRINATVAPVYDYVLATEPLNDDQLAAIGWANRQGVADTTNQFHYYRLAAD
                     RRIVWGGYDAVYHYGSRIDPALDQRESTHRVLAQHFFETFPQLEGLRFTHRWGGVIDT
                     STRFCVSFGTAFDGRVAYAVGYTGLGVGATRFGARVCLDLLYRPDSERLSLSLVRKRP
                     IPFPPEPIRWAGIEVTRRAMARADAAHGHRGPWLRLLDRLGLGFDS"
     gene            complement(3918..4112)
                     /locus_tag="PLP95_03925"
     CDS             complement(3918..4112)
                     /locus_tag="PLP95_03925"
                     /inference="COORDINATES: protein motif:HMM:NF025379.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HPB44980.1"
                     /translation="MDLGKITDKAKDLLGQNEEKIEDAVEKVADFIDEKTGHKHSDKI
                     DGAVDKIKDVVGEPEPKPEA"
     gene            4277..>5672
                     /locus_tag="PLP95_03930"
     CDS             4277..>5672
                     /locus_tag="PLP95_03930"
                     /inference="COORDINATES: protein motif:HMM:NF014044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HPB44981.1"
                     /translation="MSTRPEGMYVGALTDPATHERSETPLGINPDDLTTHGVIVGMTG
                     SGKTGLGIVLLEEALQHDIPTLILDPKGDMGNLLLSFPSLAPEEFAPWVPSGEDAAAV
                     AATWSEGLGGWGLGTEDIVRLRSGHRMCVYTPGSTAGVPINIVGSLAPPTNIAIDDEA
                     IHDEIEALTQGLLGLVNITSDPLSGREHVLIANLVHQAWSAGETLDLATLLGRIQDPP
                     MRKLGVIEIDTFFPRADRAALMMKLNGLLASPSFAAWGAGQAIDIDSMLWAPDGSPNA
                     AVVYLAHLSDEARQMVVTRILSKLVSWMRGQQGSPKLRVLVYMDEVYGFVPPTAAPPS
                     KKPILTLFKQARAFGVGVVLATQNPVDLDYKAISNAGTWMVGRLQTERDKARLLDGMS
                     SAAGTVDLRSVDATISGLAKREFLLHTTGGKAPRTFAVRWAMSYLAGPLSSDQVGRLP
                     GMAELKNAPTAAPAA"
BASE COUNT          953 a         1892 c         1865 g          962 t
ORIGIN      
        1 cgtacgaact cgaccgatgg cgcgaccaac aggcggcgat ttacgggtac cgcaccgttc
       61 gcttcaccca ccgtcaagtc accacccagc cggactatgt ccgggcgacg atggctgcgc
      121 tcctcgacgc accaacgttt ggcacactcg caacgcaacc ggcgccgccg acttcgcctg
      181 cccacagttg aactgtgggc aaccccgacc agctggtggc cccgtttgcc cacagttgaa
      241 ctgtgggcag ggtcgggccg ggtgaggggt gcagcgagag aaggtcgggg cgggtgaggc
      301 cagggcgggt gaggggtgca gcgaggtgac gagcctcagg agttgagggc ggcgatcgcc
      361 tcggcgatgg cgagggcgcg tcgagcgttg tcgacgtgca ggttctcgat catcttgccg
      421 tcgacggtga cgacgccctt gccctcggcg atgcccgcct cgaacgcctc gatgacgcgc
      481 cgcgagtagt cgatctcgtc ctcggacggg gcgaaggcct cgttgcacgg ctcgacctgg
      541 ctcgggtgca cgagggtctt gccgtcgaag cccatctcgg caccctgctg ggcctcgatc
      601 gcgaaccctt cgggattgcg aacgtcgttg tagaccccgt cgaggatcac cttgccggca
      661 tatcgcgccg cgttgacgca ccggccgagt ccccacaaca gcggatgacg ccccggcacc
      721 aacccggcgc gcaactcctt ggcgaggtcg ttggtcccca tgaccaacac ggcgagacgc
      781 tcggatgccg tcgcgatctc cacggcgttc tcgaccgcgg tcggggtctc gagcatcgcc
      841 cagatacgcg tgttctcggc cccggcaacc gattcgagta aacgctcgat ggtcgcaacg
      901 tcggcggccg agttcacctt cggcacgacg acgccatggg gacccgccgc gcccgccgaa
      961 cgaaggtcgt cctcgaacca ctgcgtatcg aggccgttga cccgaatggt cagctctcgc
     1021 ttgccgtact cccccgaacg caccgcgtcg gcgaccttgg cgcgggcgac ctctttcatg
     1081 tccggggcaa ccgagtcctc ggtgtcgaag atgatggcgt cggtcgcaag gcccttggcc
     1141 ttctcgagtg ccttgtcgtt ggccccgggc atgtagagca cggagcgccg tggaacgatc
     1201 acttcagtca tgtcgcgatc ctcctcagaa gccgtagagc tcggcgagct cagggtcacg
     1261 ggcggcgagc atcttggcga ggttgaccat cacgtggcac tgcttcaccg aggcgtcgtc
     1321 ttccatcttc ccgtcgatca tcaccgcgcc ggtgccgtcg cccatctcct cgatgacgtg
     1381 cagggcgtga tcgatgtcct ccttggaggg cgagaacacc ttcttggcga tgtcgacctg
     1441 aaccgggtga agcgaccatg ctccgacgca gccgagcagg aacgcgttgc ggaactgatc
     1501 ctcgcaggca accgtgtcct tgatgtcgcc gaagggcccg tagaacggca ggatgcccgc
     1561 catcgcgcag gcatctacca tgcgcgagat cgtgtaatgc cacaggtcct gttgatagga
     1621 ggagcgctcg gcgtcgtaga ccggtccgtc ctcgccgcga ggcgggtctt ggcgcacgag
     1681 gtatccgggg tggccgccgc cgacccgggt ggtcttcatc ctccgcgagg ccgcgaggtc
     1741 ggcgggcccg agcgagatgc cctgcatgcg ggggctggcg agtgcgatct cctcgacgtt
     1801 ggcgacgcca cgggcggtct ccaagatggc gtggagcagc aacggcttgg tcaactcggc
     1861 gcgcgcctcg agctgggcga ggaggcgatc gacgtagtgg atgtcctcgg ggccctcgac
     1921 cttggggatc atgatgacgt cgaggcgatc gcccacttcg gtgacgagcg tgatgaggtc
     1981 gtcgagcacc cagggagagt cgagcgagtt cacccgggtc cacagttgcg cgtcgccgag
     2041 atccatctcc ttggcgacct tcaccagccc ggcgcgggcg ttctccttct gatccgccgg
     2101 gatggcgtct tcgagattgc cgagcaggat gtcgaccttg gtcgccatct ccggcagctt
     2161 cgcggtcacc ttgtcgaggt gcggggggaa gaagtgaatc atccgcgacg gcgggaacgg
     2221 gatctcggtg acgggctggg gggcaccggc ggcgagcggc ttgaagaaat ccttggcact
     2281 acgcatatgc accaagctac gccgcgtccg cagatccggg cgaagatgaa cgcgccctca
     2341 cgtctcagac ggcggctccg tttcagacgg tggctccgtt tcgctcagga atcgaacccg
     2401 agccccaaac ggtcgagcag acgcagccac gggccccgat ggccgtgcgc ggcatcggcg
     2461 cgcgccatcg ctcgccgagt gacctcgatg cccgcccagc ggatcggctc cggcggaaac
     2521 gggatcgggc gtttacgcac gagcgacagc gacaaacgtt ccgagtcggg tcgatacaac
     2581 aggtccagac acacccgggc gccgaaccgg gtggccccga cgccgagtcc cgtgtagccg
     2641 accgcatagg cgacccgacc gtcgaatgcc gtaccgaagc tcacgcagaa cctcgtgctg
     2701 gtgtcgatga cgccacccca gcgatgagtg aaacgaagcc cctcgagttg cgggaacgtc
     2761 tcgaagaagt gctgcgccaa cacccggtgg gtcgactcgc gttggtcgag tgccggatcg
     2821 atgcgcgacc cgtagtggta caccgcgtca tagccgcccc acacgatccg acgatcggcc
     2881 gcgagccggt agtagtggaa ctggttcgtg gtgtcggcca ccccttgtcg attcgcccac
     2941 ccgatcgcgg cgagctggtc gtcgttcaag ggttcggtgg ccaacacgta gtcatagacg
     3001 ggcgcgaccg tggcgttgat ccgcctcagc ggcgaccgga acgcgttggt ggccaagatg
     3061 accgccccac agcgcagggc gccgcggtcg gttcgcaccg ccatgccggc accgtgacgt
     3121 tcgaggccga ccatcgtcgt gcgctcatgg atccgcactc cgagcgacct cgctgctgcg
     3181 gcgagtcccc aaccgagccg ggccgggtcg acgagcgcct cgccgtcgcg ctgccacaac
     3241 cctgcgtgat aggtgggcga atcgagttcc gaacgggtcg cttcggcgtc gagcacgtcg
     3301 atgtcgtagc cgtgcttgcg cgacagctcg gcgctggcgt ggaggtcgtc gacgagccat
     3361 ggagcggtgg cgaccgtgag ctgtccgggc tcgtgccaat cgcagtcgat gccgtgatgt
     3421 tcgatcgtgt cgcgaatgcc cgcgaagctc gcccgcccct cggcttcgat gcgcttgaga
     3481 tcctcgccga atcgactcgc cccgttgtcg agtccgtggg tcaacgacga tgaacagaac
     3541 ccaccgttgc gcccgctggc ctgttccgcg atccggcccg actggatgac gacgacgctg
     3601 cggttcggat cgtcctgcat cgattggatc gctgcccaca aaccgctgta gccaccaccc
     3661 acgatgacga ggtcgaccgc gtcatcggcg ccatttcccg tctgtccgag tgcggggccc
     3721 gcctcggggc gatcctcgcg gtcgagccag agcacggttg gcgaagcatc ggccaacgcg
     3781 tgatcgacca tggaagggcc gcggaccgct cccgcacggc gagacgccga cgcgaagccg
     3841 gacggagacg gcgtcgaggc cgaggtggca cccatcgtcg aaacgatagc cccgcggcct
     3901 cgacgggcct cacatggtca ggcttcgggc ttgggttccg gttcgccgac cacgtctttg
     3961 atcttgtcga cggcaccgtc gatcttgtcg ctgtgcttgt ggccggtctt ctcgtcgatg
     4021 aagtccgcca ccttttcgac ggcgtcttcg atcttctcct cgttctggcc gagaagatcc
     4081 ttggccttgt cggtgatctt gccgagatcc atttccaact ccttgtcggt ggcgcgacac
     4141 ggtgtcgctc gaccacggat gagattcttt caccagacgg acccgatggc gacgcgagcc
     4201 gcttcctctg ggcgtaatca cccacgcacg ccgtcgtgta cgccaccgga tggcgaaagc
     4261 tgctacgaac aactgcatgt cgacacgacc cgaaggaatg tacgtcggtg cgctcaccga
     4321 cccggctact cacgaacgca gcgagacgcc cttgggtatc aatcccgacg acctgaccac
     4381 ccacggggtc atcgtcggca tgaccggatc gggcaagacc ggcctcggaa tcgtgttgtt
     4441 ggaggaggcg ctgcaacacg acatccccac gctcatcctc gatccgaagg gtgacatggg
     4501 caacctcttg ttgagcttcc cgtcgctcgc ccccgaggaa ttcgcacctt gggtgccatc
     4561 cggcgaggac gccgctgcgg tggcggcgac ctggtccgag ggactcggcg gttgggggct
     4621 cgggaccgag gacatcgtgc gacttcgaag tggtcatcgc atgtgcgtgt acacgccggg
     4681 cagcaccgca ggagttccga tcaacatcgt cgggtcgctc gcaccgccga ccaacatcgc
     4741 catcgacgac gaagcgattc acgacgagat cgaagcgctc acccagggcc tcctcgggct
     4801 agtgaacatc acctcagatc cgctgagcgg ccgcgagcac gtcctcatcg ccaacctcgt
     4861 ccaccaggcc tggtcggcgg gcgaaaccct cgatctcgcc accctgctcg ggcggatcca
     4921 ggacccgccg atgcgcaaac tcggggtgat cgagatcgac acgttctttc cccgagccga
     4981 tcgtgccgcg ctgatgatga agctcaatgg cctgctcgcc tcgccctcgt tcgccgcctg
     5041 gggggcaggg caggccatcg acatcgactc gatgctgtgg gcacccgacg ggtcgccgaa
     5101 cgcagcggtg gtgtacctcg cgcacctctc cgacgaggcg cgccagatgg tcgtcacccg
     5161 gatcctgtcg aagctcgtga gctggatgcg cggacaacaa ggttcaccca aactccgggt
     5221 cctcgtctac atggacgagg tgtacgggtt cgtgccaccc accgccgctc ccccgtcgaa
     5281 aaagcccatc ctgacgctgt tcaagcaggc tcgggcgttc ggggtcggtg tggtgttggc
     5341 gacacagaac ccggtcgatc tcgactacaa ggccatctcg aatgcgggca catggatggt
     5401 ggggcgcctg cagaccgagc gcgacaaggc ccgactgctc gatggaatga gcagcgcggc
     5461 cggcacggtc gatctccggt cggtcgacgc cacgatctcg gggctcgcca aacgcgagtt
     5521 cctgttacac accaccgggg gcaaggcgcc gcggaccttc gcggtgcgat gggccatgtc
     5581 gtatctggcc ggcccgttgt cgagcgatca ggtcggccga ctgcccggca tggcggaact
     5641 caagaacgca ccgacagcag cgccggctgc cg
//
LOCUS       DAOOMA010000094         5639 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_17197_length_5639_cov_4.360315, whole genome shotgun sequence.
ACCESSION   DAOOMA010000094 DAOOMA010000000
VERSION     DAOOMA010000094.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 5639)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5639)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5639
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17197_length_5639_cov_4.360315"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            237..1142
                     /locus_tag="PLP95_03935"
     CDS             237..1142
                     /locus_tag="PLP95_03935"
                     /inference="COORDINATES: protein motif:HMM:TIGR01413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Dyp-type peroxidase"
                     /protein_id="HPB44982.1"
                     /translation="MSSTVTAAGLAQPGILAFGTTNHAYIEFDLRPEADLDTLANVLG
                     NLAGAITTGAGHMVVIGLRPELLGRIQPGACPDGVHGFNEPIVGPDGFTMPATQHDLI
                     VWLAGGSRDVVFDGAKDAIAQLADHAVVAEEEAGWAYHGHRDLTGFVDGTENPALAEV
                     PDVALFSDGPAAGASVLLVQKWAHESSWSELSDADQEAAIGRTKADDIELDPKPETSH
                     AARTDQDEIGKIVRRNLGYGTASRHGTMFVGLTNDQAVMQRMLERMAGIEGPRDELTR
                     HSHADTGSYYVLPPVAMLNSHCTLE"
     gene            complement(1199..1525)
                     /locus_tag="PLP95_03940"
     CDS             complement(1199..1525)
                     /locus_tag="PLP95_03940"
                     /inference="COORDINATES: protein motif:HMM:NF017273.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HPB44983.1"
                     /translation="MPETSYIASVLAIGFAITFALRAVPFAVLEPLRHSRFVTTMAAW
                     MPAGILAILAVSTLSDSVGTSVTSRLAAASAVAVTVAVHLLSGRRTLWSVGTGTLVYI
                     VAVNLG"
     gene            complement(1518..2225)
                     /locus_tag="PLP95_03945"
     CDS             complement(1518..2225)
                     /locus_tag="PLP95_03945"
                     /inference="COORDINATES: protein motif:HMM:NF015549.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HPB44984.1"
                     /translation="MRSEMTPSRRAEVAAGVQASLAAGLGMYPIGIAFGLLVVQAGLP
                     WWVAPALSVSVYAGSLELLLIGMITTATPLATIALTTLLVNFRHVFYAFTFPLRVVRN
                     RFARIYSMYALIDEAFAVTAANPHGWTAWRLLALQMSLQTYWVGGGLTGVAIGSLLPI
                     RLEGLEFALCALFVTLTLDACRTKSELPSLLLAAASFSFARLVVPGEALFVGLVVFVA
                     SLVARYVLVGPPEPTDA"
     gene            complement(2277..2909)
                     /locus_tag="PLP95_03950"
     CDS             complement(2277..2909)
                     /locus_tag="PLP95_03950"
                     /inference="COORDINATES: protein motif:HMM:NF018184.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HPB44985.1"
                     /translation="MADKIDFKKTLDRYRARRGEFRVLDVPDATYLMIDGHGDPNTSV
                     EFTEAIETLYPVAYKIKFASKQELGRDYVVAPLEGLWWAQVMDTFTTARDKSQWEWTL
                     MLFVPDWIDRTMFDAAIATVAAKGAPKRLGDVRMAVLNEGRCVQTLHIGSFDDEAAVL
                     ERMHEEFLPAEGFQMTGKHHEIYLSDFRRVAPDKRRTILRQPVEPIDFAR"
     gene            complement(2962..4455)
                     /locus_tag="PLP95_03955"
     CDS             complement(2962..4455)
                     /locus_tag="PLP95_03955"
                     /inference="COORDINATES: protein motif:HMM:NF000658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiU family protein"
                     /protein_id="HPB44986.1"
                     /translation="MRWNTDNSFVRELPEFYAESEPTPVRAPELVVLNEQLADQLGLS
                     VDELRSPDGVAVLGGNEIPDGAHPIAQAYVGHQFGNFNPSLGDGRAHLLAEVIDRDGN
                     RYDIGLKGSGRTRFSRNGDGRAALGAMLREYIVSEAMHSLHIATTRSLAVVATGETVI
                     RQPPSPGAVLTRVAASHIRVGTFELFRTSGDAALLARLVEYVRARHYPDLEEGDAAAL
                     LEAVVGRQAELIAAWMSVGFIHGVMNTDNMTLSGETIDYGPCAFMEGYDPDAVFSSID
                     HNGRYRYRNQPQIAVWNLARLAEVLLMLTPEPSQDDIARYEAILGAFDGRYEAARLRL
                     FRAKLGIDDRSDRGGVDGDFDAADTELIDDLLVWMAAERRDFTSTFRNLAQSLRHSNS
                     NLDAIVASSRDGETFVRWRNRWLDRLGDVDRDEIAEAMDRVNPIYIPRNHLVEAALAA
                     ANKGDLAPTNALIEAIRQPFNEREGLEAFAEPAPDSFTSGYVTYCGT"
     gene            4671..4967
                     /locus_tag="PLP95_03960"
     CDS             4671..4967
                     /locus_tag="PLP95_03960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB44987.1"
                     /translation="MPGLDGLRGVLVLGILAYHLGFAPAMFATVDGFFALSGFLITGL
                     LLDRTPSNVTELTTWWGWRIRRLVPAVSLLVLTVVVVFASTSGIARDGFATMTW"
     gene            5219..>5639
                     /locus_tag="PLP95_03965"
     CDS             5219..>5639
                     /locus_tag="PLP95_03965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB44988.1"
                     /translation="MTAGDSQAFAAAHPPIPRKDLPPYIDSVIDAGVLGSGVLVRAPG
                     WTMIDPDRGGRVSGEYCIDSAEAAEILALKSKPDWMVLFSGAFERPFDYEAPDGKVYP
                     ARSQTIRDGVVEQLTFRVERARAAGVRTALVEWSCSSL"
BASE COUNT         1073 a         1918 c         1738 g          910 t
ORIGIN      
        1 acggctggct gttgccaggc ctctcgctca gcgccaccga tttgcacctg aactcggcgg
       61 gcgaacagaa gctggccgac tgcgtcatcc aatccggact cctcgaatag ccgggcgaat
      121 agctggccgt cggtccgacg accaccggaa caatcggcgg atacgattgg ccgcggatcg
      181 agtcggcgtc gcgtcctcca ggtcgttgat cccccgacgg aaacggagac caactgatga
      241 gttccacagt caccgccgcc ggtttggccc agcctggaat cttggcattc ggcacgacca
      301 accacgccta catcgagttc gaccttcgtc ccgaagccga tctcgacacc ctggccaatg
      361 tgctgggcaa tctcgctggc gctatcacca ccggtgcggg gcacatggtc gtcatcgggc
      421 tgcggccgga actgttgggg cgcatccaac cgggggcatg ccccgacggc gtccatgggt
      481 tcaacgaacc catcgtcggg cccgacggat tcacgatgcc ggcaacccaa cacgatctca
      541 tcgtgtggct cgccggcggc tcgcgcgacg tggtgtttga cggagcaaag gacgccatcg
      601 ctcagctcgc cgaccacgcc gtcgtcgcgg aggaggaagc agggtgggcc tatcacggcc
      661 accgtgacct gaccggattc gtcgacggaa cggagaaccc cgcgctcgcc gaggtaccgg
      721 acgtcgcgct cttctccgac ggacccgccg ccggagccag cgtgttgctg gttcagaaat
      781 gggcccacga atcgtcatgg tccgaactct ccgacgccga ccaggaagcg gcgattggac
      841 gtacgaaggc ggacgacatc gaactcgacc ccaagccgga gacttcccac gctgcgcgaa
      901 ccgatcagga cgagatcggc aagatcgtcc gccgcaatct cggctatggc acggcgtcac
      961 gtcacggcac gatgttcgtc gggctcacga acgaccaggc cgtcatgcag cgcatgctgg
     1021 aacgaatggc cggcatcgag ggtccgcgcg acgaattgac ccgccacagc cacgccgaca
     1081 ccggttcgta ctacgtgctt ccgcccgtgg cgatgttgaa ctcacactgc acgctggaat
     1141 agtccgtgac gattcgacag cgtggccccc tccggcccgc attctgagcg accactgctc
     1201 agccgaggtt caccgccacg atgtagacga gggttcccgt acccacactc cacagggtgc
     1261 gacgacccga caacaggtgc accgcgacgg tgaccgctac cgcagacgcc gctgcgagcc
     1321 tgctggtgac actcgtgccg accgaatccg aaagggtgct cacggcgagg atcgcgagga
     1381 tccccgctgg catccatgcc gccatcgtcg tcacgaatcg tgaatgacgc aatggctcca
     1441 gcacggcgaa ggggaccgct cgcagggcga aggtgatggc gaacccgatg gccaggaccg
     1501 aggcgatgta ggacgtctca ggcatcggtc ggctccggcg gtccgacgag cacgtaccgg
     1561 gcgaccaagc tggcgacgaa aaccaccaac cccacgaaca acgcctcgcc ggggaccacc
     1621 agtcgcgcga acgagaagct ggcggcggcc aacagcagcg atggcaactc ggacttcgtg
     1681 cggcacgcgt cgagggtcaa cgtcacaaac agtgcgcaca gcgcgaactc aaggccttcg
     1741 agacgaatcg gaagcaacga tccgatggcg acaccggtca gaccgccacc gacccaatag
     1801 gtctgcagcg acatttgcag tgccaatagc cgccacgcgg tccatccgtg agggttggcc
     1861 gcggtgacgg cgaaggcctc gtcgatgagt gcatacatcg agtagatgcg ggcgaatcga
     1921 ttccgcacga cccgcagcgg aaacgtgaag gcgtagaaca cgtgtcggaa gttcaccagc
     1981 aacgtcgtca acgcgatcgt cgccagcggc gtcgccgtcg tgatcatccc gatcagcaac
     2041 aactccagtg agccggcata gacggacacc gatagagcag gcgccaccca ccacggcagt
     2101 ccggcctgca cgaccaacaa gccaaacgcg atgccgatgg ggtacatccc gagcccggcc
     2161 gccagcgagg cctggactcc ggcagcaacc tccgcccgcc gcgagggcgt catttccgaa
     2221 cgcacgctga cactgctttc atgggacctc accagccgag ggaacgcggg ccgaactcag
     2281 cgcgcgaaat cgatcggctc gacgggctga cgcagaatcg tacgccgctt gtcgggcgcc
     2341 accctgcgga aatcgctgag gtagatctcg tggtgcttgc cggtcatctg aaacccttcg
     2401 gcggggagaa attcctcgtg catccgctcc aacacagcgg cctcgtcgtc gaacgatccg
     2461 atgtgcagcg tctgcacaca gcgcccttcg ttcagcaccg ccatccgcac atcgcccagc
     2521 cgctttggcg ctcccttcgc cgccaccgtg gcgatcgccg cgtcgaacat ggtccggtca
     2581 atccagtcgg gcacgaacag catcaacgtc cactcccact gcgacttgtc gcgcgctgtg
     2641 gtgaaggtgt ccatgacctg cgcccaccac aatccctcca gcggcgcgac gacatagtct
     2701 cgaccgagtt cctgcttact cgcgaacttg atcttgtagg caaccgggta gagcgtctcg
     2761 atcgcctcgg tgaactccac ggaggtgttc gggtcgccgt ggccgtcgat catcaggtag
     2821 gtcgcgtcgg gaacatccag gacccggaac tcgccgcgcc gggccctgta gcggtcgagc
     2881 gtcttcttga aatcgatctt gtcggccacg atcagttcct accacgaccg cgccggtcgc
     2941 tcccgacctc tccgctctcg atcaggtgcc gcagtaggtg acgtagccgg acgtgaacga
     3001 gtcgggggct ggctcggcga acgcctcaag cccctcacgt tcgttgaacg gctgtcgaat
     3061 ggcctcgatg agcgcgtttg tcggcgcgag atcgcccttg ttcgcggcag ccaacgcggc
     3121 ctccaccaga tgattgcgtg ggatgtagat cgggttcacc cgatccatcg cttcggcgat
     3181 ctcgtcgcga tcgacgtcgc cgagtcgatc caaccaacgg ttccgccacc gaacgaaggt
     3241 ctcgccatca cgtgacgacg cgacgatcgc atcgagattc gagttggagt gacgcagcga
     3301 ctgggcgagg ttgcgaaacg tcgaggtgaa gtctcgacgc tcagctgcca tccacaccag
     3361 caggtcgtcg atcagctccg tatcggcggc atcaaagtca ccgtcgacgc cacctcgatc
     3421 cgaccggtcg tcgattccca gcttggcccg aaaaagacgg agccgcgccg cctcatagcg
     3481 gccatcgaat gcgccgagta tcgcctcgta acgagcgatg tcgtcctgcg acggttccgg
     3541 ggtgagcatc aacaacacct cggcgagacg cgccaggttc cagaccgcga tctgaggttg
     3601 gttccggtag cggtagcggc cgttgtgatc gatcgaactg aacacggcgt cggggtcgta
     3661 tccctccatg aacgcacacg gcccgtagtc gatcgtctcg cccgacagcg tcatgttgtc
     3721 ggtgttcatc acgccgtgga tgaacccgac cgacatccac gcggcgatca gttccgcttg
     3781 acgaccgacc accgcctcga gcagcgcagc ggcatcaccc tcctcgaggt cgggatagtg
     3841 gcgagcgcgc acgtactcga cgaggcgagc caatagcgcc gcatcccccg aggttcggaa
     3901 gagctcgaac gtgccgacgc ggatgtgcga cgcagcgacc cgggtgagga cggcaccggg
     3961 gctcggtggt tgacgaatca ccgtctcgcc ggtggcgacc acggcgagcg agcgggtggt
     4021 cgcaatgtgt agcgagtgca tcgcctcgct gacgatgtac tcgcgcaaca tcgcgccgag
     4081 cgccgcacgt ccgtctccgt ttcgcgagaa ccgcgtgcgg cccgagccct tcagtccgat
     4141 gtcgtagcgg ttgccgtcgc ggtcgatcac ctcggccaac aagtgggcgc gaccgtcgcc
     4201 gagcgacgga ttgaagttgc cgaactggtg gccgacgtat gcctgggcga tcggatgtgc
     4261 accgtcgggg atctcattgc cgccaagcac cgccacgccg tcggggctgc ggagctcgtc
     4321 gacactcaaa ccgagttgat ccgcgagttg ttcattgagc accaccaact cgggggctcg
     4381 caccggtgtc ggctcagact cggcatagaa ctcgggaagt tctcgaacga acgaattgtc
     4441 ggtgttccaa cgcaccgctc cagcgtgcca cgccgacggt gaccaccgag cgcgccgcca
     4501 acggagcacc ccacacggat tcgaaactcc cgaaagccca ccagcaacgt cgtgaaaatc
     4561 ctccgcgtgc gggaacagaa cccaacacga tggacacctc tggtcttgcg cgacaacatc
     4621 gtgcgatgcc ggaggcgtga cccacaccga atgtcaccgc caaggtccac atgcccggcc
     4681 tcgatggtct ccgcggcgta ctggtgctcg gcatcctggc gtaccacctc gggttcgctc
     4741 ccgcgatgtt cgccacggtc gacggcttct tcgcactctc gggatttctc atcaccgggc
     4801 tgctcctcga tcgaaccccg tccaacgtca ccgaactcac cacctggtgg ggctggcgca
     4861 tccgacgtct ggttcccgct gtctcgttgc tcgtgctcac ggtcgtggtg gtcttcgcat
     4921 ccacgtccgg aatcgcccgc gacggcttcg cgacgatgac ctggtaggcg aactggaacc
     4981 agatcttcaa cggcacgtcc tattgggcac cccagccatc accgttgcga cacgcgtgga
     5041 ccctgtcgat cgaggagcag tcggcagccg cagcactcga atcggcacgc gccgcgctcg
     5101 aggccgcatc gaccgagccg ccaacaaccg aatctcctgc aacggagtcg ccgacgcccg
     5161 cgcccctcga cactgctgca ccggatacga ccgcggctcc caggcaccga tgcgcgtgat
     5221 gaccgctggc gacagtcagg cgttcgccgc cgcccacccg ccgatcccac gcaaggatct
     5281 gccgccgtac atcgactcgg tcatcgacgc cggggtgctg gggagtgggg tcctcgtccg
     5341 agccccgggt tggacgatga tcgaccccga tcggggcgga cgtgtcagcg gcgaatactg
     5401 catcgattcc gctgaggccg ccgagattct cgcgctcaag tcgaagcccg attggatggt
     5461 gctgttctcg ggggccttcg agcgaccctt cgactacgag gcccccgacg gcaaggtcta
     5521 tcccgccaga agccagacga tccgcgacgg ggtcgtggaa cagttgacct ttcgcgtcga
     5581 gcgagccagg gccgccgggg tacgcacggc gctggtcgaa tggtcgtgct cgagtctgg
//
LOCUS       DAOOMA010000095         5632 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_17228_length_5632_cov_4.321320, whole genome shotgun sequence.
ACCESSION   DAOOMA010000095 DAOOMA010000000
VERSION     DAOOMA010000095.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 5632)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5632)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5632
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17228_length_5632_cov_4.321320"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1117)
                     /locus_tag="PLP95_03970"
     CDS             complement(<1..1117)
                     /locus_tag="PLP95_03970"
                     /inference="COORDINATES: protein motif:HMM:NF017026.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HPB44989.1"
                     /translation="MDVFESPFGPLNLERTPGRCSELRGWNAAEEYVLAHLIDRFGSA
                     ALPGRIIVVNPAAGAVPLALALGGASPVVVTDSAVDVLAMQANAVCNGIDPDRFTVVA
                     DPRTLVEVTGWGEQAADVVVVMVPKGQALLEAQLREIAGVVDDHTVVVGTGMTRHVHR
                     ATIDAFADLIGPATTSLARRKARLIQPTPDPTRCGPLAPSNGRPTIEVTDHDLRLVSE
                     PGVFGAERVDAGTRLLLDHLPPLDQYAGGPDSPVRVIDLGCGNGVIGAAIARRYPGAE
                     LTLIDESYAAIRSARATFEATVGLAVDAQHQPRRRPPRFVVGDGLRAFVPGDDATPHE
                     PGADDMVADVIITNPPFHHDHAVTDEIAWSFFHGARRV"
     gene            complement(1125..2336)
                     /locus_tag="PLP95_03975"
     CDS             complement(1125..2336)
                     /locus_tag="PLP95_03975"
                     /inference="COORDINATES: protein motif:HMM:NF024647.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4041 domain-containing protein"
                     /protein_id="HPB44990.1"
                     /translation="MVSPRNEQPMVSSGWIVASDEATASAVRIEVDAATLAMYPFNPD
                     IFAPPDPSPAPPIVVALVDVVGLDQLGADVIQPLVEFTLANGETHRLRTTAKFLDEIV
                     AALEASRTGDAPDDDLDDADLDDDDLDAFLEPAKVVRQGRSVEPLSPASELASSTGPS
                     STPEPIDVASLAELPEVPPPSSSTSKRALQGEIEALRTYIAAMGVAQRSALRSDVTAL
                     TSAVERLTEHRAEVFEQVRAATAELRDLRNDYVEVAREGRLQLLGAFRFERPVDHVVP
                     PSDELVEVRRRIERRLLDDAAIRVRPDRASSAASQGASSRDLAMLLLRTYNAEADLVV
                     RGLQPFDLTVASERLEAAAQAVLRLGSDLELAIDPDFHRLRLEELRLVAEQHAAADGT
                     HSGASAPTSHF"
     gene            complement(2383..2721)
                     /gene="mnhG"
                     /locus_tag="PLP95_03980"
     CDS             complement(2383..2721)
                     /gene="mnhG"
                     /locus_tag="PLP95_03980"
                     /inference="COORDINATES: protein motif:HMM:TIGR01300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0098662 - inorganic cation transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monovalent cation/H(+) antiporter subunit G"
                     /protein_id="HPB44991.1"
                     /translation="MAQVFVLAGAVLILFSALGVTRFNDVMAQMHFLSKATTLGIVVL
                     LVGAALGMDSANDWTSLLLAAFLQMMTSPVSANLISRSTYLRERRVHEASESGAVDDV
                     STGRTEISGS"
     gene            complement(2721..3071)
                     /locus_tag="PLP95_03985"
     CDS             complement(2721..3071)
                     /locus_tag="PLP95_03985"
                     /inference="COORDINATES: protein motif:HMM:NF015993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015075 - monoatomic ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monovalent cation/H+ antiporter complex subunit
                     F"
                     /protein_id="HPB44992.1"
                     /translation="MKTITFVVLAAAGALFMLRTVRGPSLSARVIGMDGMIVVGVGAI
                     AADAMYRADGIYLPTVVVITLVGFVSTAASARFIERRELDADADADGDGVADADGVGS
                     AGSAGREDHPGGRS"
     gene            complement(3068..3598)
                     /locus_tag="PLP95_03990"
     CDS             complement(3068..3598)
                     /locus_tag="PLP95_03990"
                     /inference="COORDINATES: protein motif:HMM:NF014012.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter subunit E"
                     /protein_id="HPB44993.1"
                     /translation="MSRVTLGLGLIVGWMMLWGSASPGTVLGGIAVVLVLFSTFPSSR
                     PKLPSVRLDLVALVRLVGYFTSELVVSNLRLSVELVRPTSRLRGEVVDVEMHTTSPSV
                     ITAVSNLAAMSPGTMVVGTSQHPPVLRIHVLVFGGREGTEESVESIRTLERLSLEAFG
                     TEADRSAYQASIGGAP"
     gene            complement(3595..5118)
                     /locus_tag="PLP95_03995"
     CDS             complement(3595..5118)
                     /locus_tag="PLP95_03995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter subunit D"
                     /protein_id="HPB44994.1"
                     /translation="MNLLLAVPILLPLLGAGLSMIVGRSRAAQRTVSLTVLTVVMGCA
                     IAIAIHVDRVGPLSLQAGGWRAPIGISLVADRLSATMLAVASVMLVAVMVYAIGQPGA
                     ERNHVGFQSVYLIMAAGVAGSFLPGDLFNLFVSFEMMLAASYVLITIGARKDQVRSGM
                     SYVVISLLASTFFVVVLALVYSATGTLNLADLSVRMGELSPGLRSGFSLMMLIVFGIK
                     AGLFPLFFWLPDSYPTAPSAVTAVFAGLLTKVGVYAIIRTQTLLFDNDSRPDRLILVL
                     AAATMVVGVLGAIAQDDVKRILSFHIVSHVGFMVMGLGFFTVAGLGAALFYIVHHIVV
                     MTTLFLTGGLIEHVGGSSRLSRVGGLVRSAPGVAVLFALPALSLAGLPPFSGFVAKVG
                     LIDAGVRSERYAIIAVSLVVSLMTLFSMTKIWSAAFWGEEPELCADDLDVLDEYEGLG
                     LIPGGHDDRPPLMVIPTAVLAAISIGIAVFAGPLHDLSTRAAEGIIDPDAYYEVVEP"
     gene            complement(5115..5537)
                     /locus_tag="PLP95_04000"
     CDS             complement(5115..5537)
                     /locus_tag="PLP95_04000"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF012636.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit K"
                     /protein_id="HPB44995.1"
                     /translation="MTITLAALAALMFGIGTYLVLQRKLSRIIIGIGLLSHGANVLFL
                     GAGRSGRPPFIKEDTAAEHAALTDPLPHAMVLTAIVISFGVTALLLALAYRSWLLTDD
                     DEVEDDVSDRVVAGGGFLDREIRDADAVIASVIDPEDV"
BASE COUNT         1092 a         1937 c         1784 g          819 t
ORIGIN      
        1 acacccggcg agcaccgtgg aagaaactcc aggcgatctc atcggtgacg gcatggtcgt
       61 gatgaaacgg cgggttggtg atgatgacgt cggcgaccat atcgtcggca ccgggctcgt
      121 gtggcgtcgc gtcgtcgccc ggcacgaacg cccgcagacc gtcgcccacc acgaatcgtg
      181 gcggtcggcg ccgcggctga tgctgcgcgt cgaccgccag cccgacggtg gcctcgaacg
      241 tggcgcgggc cgaacggatc gctgcgtagg actcgtcgat caaggtgagt tccgcgcctg
      301 ggtaccgtcg tgcgatcgcc gcgccgatga cgccgttgcc gcaccccaga tcgatcaccc
      361 gaaccggcga gtcgggcccg cccgcatact ggtcgagcgg cggaaggtgg tcgagcaaca
      421 gccgtgtgcc ggcgtcgacc cgttcggcac cgaacacccc cggctcgctg acgagcctca
      481 gatcgtggtc ggtcacctcg atcgttggcc gaccgttcga cggcgcgagc ggaccgcacc
      541 gggtcgggtc cggggtcggc tgaatgagtc gggccttgcg acgcgccagc gacgtggtcg
      601 ccggcccgat caggtcagcg aacgcgtcga tggttgcccg gtggacgtgg cgggtcatgc
      661 ctgttccaac cacgaccgtg tgatcgtcga caactccggc gatctctcgc agctgggctt
      721 cgagcaacgc ttggcctttc ggcaccatca cgacgacgac gtcggccgcc tgctcgcccc
      781 aaccggtgac ctccacgagg gtccggggat cggcgacgac cgtgaatcgg tccggatcga
      841 tgccgttgca aaccgcgttg gcctgcatcg ccaacacatc gacggcgctg tcggtgacca
      901 cgaccggcga tgccccaccc aacgcaagtg cgagcggcac cgcgccggct gcagggttga
      961 ccacgatgat gcgcccaggg agcgctgccg agccgaagcg atcgatgaga tgcgccaaca
     1021 catactcctc ggcggcgttc cagccgcgaa gctcgctgca gcgtcccggg gtccgttcga
     1081 ggttcagcgg cccgaacgga ctctcgaaaa cgtccacccg acgctcagaa gtgcgaggtc
     1141 ggagcggatg cgcccgagtg ggtgccatca gctgccgcat gctgttcggc gacgagacgc
     1201 aactcctcga gacgcaaccg gtgaaagtcc gggtcaatcg ccaactcgag atccgagccg
     1261 agccgaagca ccgcctgagc agcagcctcc agtcgttcgc ttgcaacggt gagatcgaat
     1321 ggctgcagcc ctcgcaccac aaggtccgcc tcggcgttgt aggtgcgcaa cagcaacatc
     1381 gccaggtcac gagacgacgc gccctgtgac gcggcgctcg acgcgcggtc gggtcggact
     1441 cgaatagcgg catcgtcgag taggcgacgt tcgattcggc gacgcacctc taccaactcg
     1501 tcgctcggcg ggaccacgtg atcgaccggt cgctcgaatc ggaaggcgcc aagcagttgc
     1561 agccggcctt cccgtgcgac ctccacatag tcgttgcgta gatcccgcaa ctcggccgtc
     1621 gctgctcgaa cctgctcgaa gacctccgcg cgatgctccg tcagccgttc gacagcggag
     1681 gtgagcgccg tcacgtcaga tcgcaacgcc gaacgctgcg ccacacccat cgctgcgatg
     1741 taggtgcgga gcgcctcgat ctcgccttga agcgcacgct tggaggtgct cgaactcggc
     1801 ggcggaacct cgggtagctc ggccaggctt gcgacgtcga ttggctccgg agtcgatgag
     1861 ggacccgtgg acgaggcgag ctcggaggcc ggcgatagcg gctcgaccga ccgtccctgc
     1921 ctcaccacct tggccggctc gaggaacgcg tcgagatcgt cgtcatccag atcggcgtca
     1981 tcgagatcgt cgtcaggcgc gtcgccggtt cgcgacgctt cgagcgccgc gacgatctca
     2041 tcgaggaact tcgcggtcgt gcgtagacgg tgcgtctcgc cgttcgccag cgtgaactcg
     2101 acgagcggct ggatcacatc ggcacccaac tgatccaggc caacgacatc gacaagcgcc
     2161 acgacgatcg gcggagccgg actcgggtcg ggcggcgcga aaatgtcggg attgaacggg
     2221 tacatggcca acgtggccgc atcgacctcg atccgcacgg cgctcgccgt cgcctcgtca
     2281 ctcgcgacaa tccaccccga tgacaccatc ggctgctcgt ttcgggggga aaccatggga
     2341 cccatgttac gaaggcgccg ccgccgatcg gcgcacttcg gctcaacttc ccgagatttc
     2401 cgtccgcccc gtcgacacgt cgtcgaccgc acccgactcc gaggcctcat gcacccgtcg
     2461 ctcgcgcaga taggtcgacc ggctgatgag atttgccgac accggggagg tcatcatctg
     2521 caagaacgcc gccagcaaca gcgacgtcca gtcgtttgcg gagtccattc ccagcgccgc
     2581 accgacgagc aacaccacga tcccgagcgt ggtggctttg gacaggaagt gcatctgggc
     2641 catcacatcg ttgaaccgcg tcaccccgag cgccgagaac aggatcaaca cggccccggc
     2701 caacacgaaa acctgcgcca tcacgaccga ccccccggat ggtcctcacg cccagccgaa
     2761 ccagccgaac ccacaccatc ggcgtcggcc acaccatcgc catcggcgtc ggcatccgcg
     2821 tcgagttcgc gacgttcgat gaaccgagcc gatgctgcgg tgctcacgaa acctacgagc
     2881 gtgatgacga cgacggtcgg caggtagatg ccgtcggcgc gatacatcgc atcggcggcg
     2941 atcgctccga cccccacgac gatcatgccg tccataccga tgacccgcgc cgacaacgac
     3001 ggtccccgca cggtcctcaa catgaacagt gccccggccg cggcgagcac cacgaacgtg
     3061 atcgtcttca cggcgcacct ccgatgctgg cctggtaggc gctccggtcg gcctcggtcc
     3121 cgaacgcctc gagcgagagt cgttccaacg tccggatcga ctcgaccgat tcctcggtgc
     3181 cctcgcgccc gccgaagacc agcacgtgaa tccgcagaac cggcggatgt tggctcgtgc
     3241 ccacgaccat cgttcccggc gacatcgcgg ccaggttcga caccgcggtg atgaccgacg
     3301 gcgacgtcgt gtgcatctcc acgtcgacca cttcgccgcg aagcctcgag gtcggtcgga
     3361 ccaactcgac gctcaggcgc aggttcgaca ccacgagctc cgaggtgaaa tacccgacga
     3421 ggcgtaccag ggcgacgagg tcgagtcgca cgctcggcaa tttcggtcgc gaactgggga
     3481 aggtcgaaaa caacaccagg acaacggcga tgccgccgag cacggttccc ggactcgcgc
     3541 tcccccagag catcatccac ccgacgatga ggccgagccc gagcgtgacc cggctcacgg
     3601 ctccaccacc tcgtaatagg cgtctgggtc aatgatgcct tcagccgctc gggtgctgag
     3661 atcgtgcaac ggtccggcga acacggcgat tccgatgctg atcgcggcga gcacggccgt
     3721 ggggatcacc atgaggggcg ggcgatcgtc gtggcccccg ggaatgaggc cgaggccctc
     3781 gtactcgtcg agaacgtcga ggtcgtcggc gcacaactcc ggctcttcac cccagaacgc
     3841 cgccgaccag atcttggtca tcgaaaacag cgtcatgagg ctcacgacga gcgacaccgc
     3901 gatgatggcg tagcgctcgc tgcgaacgcc ggcatcgatc agcccgacct tggcgacaaa
     3961 cccggaaaac ggcggcagcc cggcaaggct gagcgctgga agcgcgaaga gcaccgccac
     4021 ccccggcgcc gaacggacca ggccgccgac ccgactcagt cggctcgacc caccgacgtg
     4081 ttcgatgagg ccgccggtga ggaacagcgt cgtcatcacc acgatgtgat ggacgatgta
     4141 gaacagcgcc gcgcccaggc ctgcgacggt gaagaatccg aggcccatca ccatgaaccc
     4201 gacgtggctc acgatgtgga acgacaagat tcgtttgacg tcgtcttggg cgatcgcccc
     4261 caacaccccc acgaccatcg tcgctgcggc caacacgagg atcagtcgat cgggccggct
     4321 gtcgttgtcg aacaacaacg tctgggtgcg gatgatggcg tagacgccga ccttggtcag
     4381 caacccggcg aacaccgccg tgaccgccga aggtgccgtc gggtagctgt cgggcagcca
     4441 gaagaacagc ggaaacagcc ccgccttgat gccgaacacg atcagcatca tcaacgagaa
     4501 tccgctgcgc agaccgggcg acagttcgcc catgcgaacc gacaggtcgg cgagattcaa
     4561 cgttccggtg gccgagtaga ccagcgccaa caccacgacg aagaacgtcg acgccagcag
     4621 cgagatcacg acatagctca tcccggaccg aacctgatcc ttacgggccc cgatcgtgat
     4681 cagcacgtaa ctggcggcga gcatcatttc gaaactgacg aagaggttga ataggtcgcc
     4741 ggggagaaac gacccggcaa cccctgcggc catgatgaga tacaccgact ggaacccgac
     4801 gtgattgcgc tcagcgccgg gctggccgat ggcgtacacc atcaccgcca ccagcatcac
     4861 cgacgccacc gccagcatgg tcgccgatag gcgatccgcg accaggctga tgccgatcgg
     4921 cgcccgccaa ccgccggctt gcaggctcag cgggccgact cgatcgacgt gaatcgcgat
     4981 cgcgatggcg cagcccatga cgacggtgag caccgtgagg ctgacggttc gctgggcggc
     5041 gcgactccga ccgacgatca tcgacagccc ggcgcccaac agcggcaaca ggatcggaac
     5101 agccaacagc aggttcacac gtcctccggg tcgatcacgc tcgcgatgac cgcgtcggca
     5161 tcgcgaatct cccggtcgag gaatccgccg ccggcgacga cgcggtcgga cacgtcgtct
     5221 tcgacctcat cgtcgtcggt caacaaccag cttcggtacg ccagggccaa caacagcgcg
     5281 gttacgccga aggagatgac gatggcggtc agcaccatgg cgtgtggcaa cgggtcggtg
     5341 agggccgcgt gttcggcggc ggtgtcctcc ttgatgaacg gcggacggcc gcttcggccg
     5401 gcgccgagga acaacacgtt cgcaccgtgg ctcaagagtc cgatgccgat gatgatgcgg
     5461 ctgagcttgc gttgcagcac caggtaggtg ccgatgccga acatgagcgc ggccagcgcg
     5521 gcgagggtga tggtcataac acgtcccatc cgtcgacaaa acgctcaccg aacgcctcga
     5581 ccaccatgag caccaggccg atcaccacga ggtcgacgcc gccgtcgaac gc
//
LOCUS       DAOOMA010000096         5597 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_17374_length_5597_cov_3.995128, whole genome shotgun sequence.
ACCESSION   DAOOMA010000096 DAOOMA010000000
VERSION     DAOOMA010000096.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 5597)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5597)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5597
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17374_length_5597_cov_3.995128"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1583
                     /gene="ppk1"
                     /locus_tag="PLP95_04005"
     CDS             <1..1583
                     /gene="ppk1"
                     /locus_tag="PLP95_04005"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012224036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009358 - polyphosphate kinase complex
                     [Evidence IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006799 - polyphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HPB44996.1"
                     /translation="RVVDPDDSEERFARLKIPPALPRYIPTLDENRLLPIEELVSAHL
                     DRLFIGMKIEEYQTFRVTRNADLDLSEEDADDLLELVEMEIRRRRFGDAVRLEVPTSM
                     SDEMVSLLQRELEVSDVHRHAVPLDLSGLWVVAGLDRPELKFNHVPAVDSRALAGSPG
                     LDNDVFAILRRGDVLVHHPYESFSSSVEEFIRQAAIDRQVLAIKVTLYRTSGDSAIVA
                     SLVRAAEAGKQVVALVELKARFDEENNIAWARRLERAGVHVVYGLVGLKTHTKITMVA
                     RDEPDRVRVYCHVGTGNYNSRTARIYEDFGLFTASHDVADDVQNLFNYLTGYSRSVDY
                     RRLLVAPDELRSRIVSLIRNERPAPIGTTEAGAGRIVMKMNSLVDPEMIDELYAASAD
                     GVQIDLIVRGICCLRPQVPGMSDNIRVRSIVGRFLEHSRIYRFANGTGPGEAALYLGS
                     ADLMPRNLDGRVEALIGVTNPALVDRIDSVLDIELADDMLAWELRDDTWAKVPTSVGV
                     DAHVQLTELSRTWVSQAR"
     gene            1598..2011
                     /locus_tag="PLP95_04010"
     CDS             1598..2011
                     /locus_tag="PLP95_04010"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HPB44997.1"
                     /translation="MAKQYGPDGVLEAAGGVIIDDGGRVAAIYRPHRNDWSLPKGHLD
                     PGEDAKAGALREVFEETGLRCRIDSTGPEVRYVDGKGRDKRVRYFLMSVLDGEFRAND
                     EATELVWLDDDLALLTYEVDRRLIADARRPPKDRA"
     gene            complement(2018..2344)
                     /locus_tag="PLP95_04015"
     CDS             complement(2018..2344)
                     /locus_tag="PLP95_04015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB44998.1"
                     /translation="MSDYAPESTEPPTAAVTVVYLGPVAPHWDVRSDFGDRAVVDEFF
                     RRLNARLMLVPPHDPQFRRNRERVIRDAERENILLTWDPPELGEPFPPRDRDRDDRDR
                     DRRRRD"
     gene            complement(2411..3559)
                     /locus_tag="PLP95_04020"
     CDS             complement(2411..3559)
                     /locus_tag="PLP95_04020"
                     /inference="COORDINATES: protein motif:HMM:NF012318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HPB44999.1"
                     /translation="MPSAPETRPGKGRNWWGALVLGALIGAIVASIVSGAIYTASVRT
                     SDSATSGSTSNPGGNTSLDRAPTPTIVGEPLDIGSILDKARPSIVSIRIESSSVEASG
                     SGIVIDDAGTVLTNAHVISGTDGLSSAITVQFHDGASVPAQLVGSFPDQDVAIVRTVE
                     PHQSTPAELGSSEALTVGEDVIAIGNALNLGAQPSVTRGIVSALNRTLAAESVVLENL
                     IQTDAAINPGNSGGALVNSYGQVVGVNTAIINNSQSLGFALSIDSVKPLIEEILAGRG
                     DVDGDSPFLGVTTLDVQSQQSDVLARYGVVTDHGAFVTSVTAGTAAANAGMQPGDVIV
                     RVDGQQVESNIDVGTIIRGFDAGDSVEIVVERRGREMTLNATLGRRGG"
     assembly_gap    3772..3871
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            4290..4562
                     /locus_tag="PLP95_04025"
     CDS             4290..4562
                     /locus_tag="PLP95_04025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WhiB family transcriptional regulator"
                     /protein_id="HPB45000.1"
                     /translation="MYGTDYSDTSWMSQGNCSHRDPAMFFPSDGVGVEIARKACQGCP
                     VIAECLEYAISERIDHGVWGGCSERERRRIIRARAQAAKAQPVTIG"
     gene            complement(4687..4863)
                     /locus_tag="PLP95_04030"
     CDS             complement(4687..4863)
                     /locus_tag="PLP95_04030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HPB45001.1"
                     /translation="MPQGSEWIFIIIAVVVLFGGSQIPKLARSLGEAKKEFKQGLDEG
                     ASEESDDKADRASS"
     gene            4957..5577
                     /locus_tag="PLP95_04035"
     CDS             4957..5577
                     /locus_tag="PLP95_04035"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR02727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030272 - 5-formyltetrahydrofolate
                     cyclo-ligase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HPB45002.1"
                     /translation="MAWPSPDSPNSERDAQPTATDLRMTMRRRRRELDDAEQHSAAEA
                     IAVHLLSDRRVSEARRVGVYLAFDGEVRLDQAIEALQRRGIEVAAAVLGDGRLRFASI
                     GPDTPWRTNRYGIDEPDIPAALTLDGGEIDVVVVPAVAVDLRGNRVGMGAGWYDRTFA
                     GRSGESPLLVATVHDFQVVAGIASQPHDVAVDAIVTPTRWIDATLG"
BASE COUNT          916 a         1827 c         1847 g          907 t
ORIGIN      
        1 tgcgcgtcgt cgaccccgac gactccgagg agcgattcgc ccgcctcaag atcccgccag
       61 cgctgccccg ctacatcccc acgctcgacg agaaccggct gttgcccatc gaggaactcg
      121 tttccgccca cctcgaccgc ctgttcatcg gcatgaagat cgaggagtac cagaccttcc
      181 gggtgacccg aaacgccgac ctcgacctgt ccgaagagga cgcggacgat ctgttggagc
      241 tcgtcgagat ggagatccgt cgccgacgct tcggcgacgc ggttcgactc gaggtaccca
      301 cctcgatgtc ggatgagatg gtgtcgttgc tgcaacgcga actcgaggtg agcgatgtgc
      361 accgccacgc cgtcccgctc gacctgtccg ggctctgggt ggtggccggg ctcgatcgac
      421 cggaactgaa gttcaaccac gtacccgccg tcgattcgcg tgctctggcg ggaagcccgg
      481 gcctagacaa tgacgtgttc gccatcttgc gtcgtggcga cgtgctggtg catcacccct
      541 atgagagctt ctcgagttcg gtcgaggagt tcatccgcca agcggccatc gaccgtcagg
      601 tgctggccat caaggtgacc ctgtatcgca cctccggcga ctcggcgatc gtcgcgtcgc
      661 tcgtccgcgc cgccgaggcg ggcaagcagg tggtggccct ggtcgaattg aaggcccgtt
      721 tcgacgagga gaacaacatc gcctgggcgc gtcgcctcga acgagccggg gtgcacgtcg
      781 tgtacggcct cgtcgggctc aagacgcaca ccaagatcac gatggtcgcc cgcgacgaac
      841 ccgaccgggt gcgcgtctac tgccatgtcg gcaccggcaa ctacaactcc cgaaccgcca
      901 ggatttacga ggacttcggg ttgttcaccg cgtcccacga cgtcgccgac gacgtgcaga
      961 acctgttcaa ctacctgacc ggatacagcc gttcggtcga ctaccggcgc ctgctcgtgg
     1021 ctcccgacga actgcgcagt cgaatcgtgt cgctcattcg caacgagcgc ccggcaccga
     1081 tcggcaccac cgaggccggg gcggggcgca tcgtcatgaa gatgaacagc ctcgtcgacc
     1141 ccgagatgat cgacgagttg tacgcagcct cggccgatgg ggtccagatc gacctgatcg
     1201 tgcgcggcat ctgctgcctg cgccctcagg tgccggggat gtcggacaac atccgggtgc
     1261 gcagcatcgt gggccggttc ctcgagcact cgcggatcta ccgcttcgcg aacggcaccg
     1321 gccctggcga ggcggcgctg tatctcggtt cggccgacct gatgccccgc aacctggacg
     1381 gacgggtcga ggcgctgatc ggcgtcacga acccggcgtt ggtcgaccgt atcgactcgg
     1441 tgctcgacat cgagctggcc gacgacatgc tggcctggga gctgcgcgac gacacctggg
     1501 cgaaggttcc cacgagtgtc ggcgtcgatg cccacgtgca actgaccgag ctttcgagaa
     1561 cgtgggtcag ccaagctcga taacgtcgct gggcgttatg gccaagcaat acggacccga
     1621 tggggtgttg gaggctgcgg gcggtgtcat catcgacgat ggcggccgtg tcgccgcgat
     1681 ctaccgacca caccgaaacg actggtcgtt gcccaaaggg catctcgatc ccggcgagga
     1741 cgccaaggcg ggcgcactcc gcgaggtttt cgaagagacc gggctgcgct gccgcatcga
     1801 ctcgacggga ccggaggtgc gctatgtcga cggcaagggg cgcgacaaac gggtcaggta
     1861 cttcctgatg tcggtgctcg acggtgagtt ccgtgccaac gacgaggcca ccgagttggt
     1921 gtggctcgac gacgacttgg cgctgctcac ctacgaggtg gaccgccgac tcatcgccga
     1981 cgcgcgccgc ccgccaaagg accgggcgta gcgggtttca gtcgcgacgt cggcggtcgc
     2041 gatcgcggtc gtcacggtcg cggtcgcggg gtgggaacgg ctcgccgagc tccggcggat
     2101 cccaggtcag caggatgttc tcgcgctccg catcgcgaat gactcgttcg cggttgcggc
     2161 gaaactgcgg gtcgtgcgga ggcaccagca tcagccgggc gttcagccga cggaagaact
     2221 cgtccaccac ggcgcggtcg ccgaagtctg atcgcacgtc ccaatggggt gcgaccggac
     2281 ccagatacac cacggtcact gccgccgtcg gcggctcggt ggactctggt gcgtagtcag
     2341 acatcggtgt ctcctggttc gacgggcgcc agcggactgc cgcgggcgcc cacagcttac
     2401 cgactgtcga tcacccgccg cggcggccca gtgtcgcgtt gagggtcatc tcccggcctc
     2461 gacgctccac gacgatctcg accgagtccc cggcgtcgaa tccacgaatg atcgtgccga
     2521 cgtcgatgtt ggactcgacc tgttgaccgt cgaccctgac gatgacgtca ccgggttgca
     2581 tcccggcgtt cgcggccgcg gttcccgcgg ttaccgacgt cacgaacgcg ccatggtcgg
     2641 tgaccacgcc gtaacgagcc aacacgtctg actgctggct ctggacatcg agagtcgtga
     2701 cgccgagaaa cgggctgtcg ccgtcgacat cgccgcgccc ggcgaggatt tcctcgatca
     2761 acggcttcac cgaatcgatc gacaacgcga agcccaggct ctgagagttg ttgatgatcg
     2821 ccgtgttcac cccgacgacc tgaccgtagc tgttcacgag cgcgccgccc gagtttccgg
     2881 ggttgatggc cgcgtcggtt tggatgaggt tctcgaggac gacgctttcg gcggccagcg
     2941 tccggttgag ggcggaaacg atgccccgcg tcacgctcgg ctgcgcgccg agattcaacg
     3001 cgtttccgat ggcgatgacg tcctcaccca ccgtcagcgc ctcggacgat ccgagttccg
     3061 ccggcgtcga ctgatggggt tcgaccgtgc ggacgatggc gacgtcctga tcggggaaac
     3121 tgcccacgag ctgggccggc accgacgccc cgtcgtggaa ctgcacggtg atcgcgctcg
     3181 acaacccgtc ggttccggag atcacgtgcg cattggtcaa cacggttccg gcatcgtcga
     3241 tgacgatacc cgatccgctg gcctcgacgc tcgaggactc gatccgaatc gacacgatcg
     3301 acggccgcgc cttgtcgaga atgctcccga tgtcgagcgg ctcgccgacg atggtcgggg
     3361 tcggggcgcg atccagcgac gtgttgccgc ccgggttcga tgtggacccc gacgtcgccg
     3421 agtcggaggt gcgcaccgac gccgtgtaga tcgctccgga cacgatcgag gccacgatcg
     3481 ctccgatcag cgcaccgagc acgagcgcac cccaccagtt gcgacccttg cccggcctgg
     3541 tttctggtgc cgacggcatc ggccgtggcg acacaagcag gggggccgcc ggtccgttgg
     3601 ttccggtgag atacagcggc ggcgctccat cgaacgcgga tcgtttgtcg cgatcgtgcg
     3661 tcggcgcggg cgtgggcgtc ggagaccccg gtgggtgaac gccgggtggc gcataccgcg
     3721 gtgattctgc acccagtgga gcggctggcg gtggcccgct ggctggaggc gnnnnnnnnn
     3781 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3841 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ncgcggtgat tctgcaccca gtggagcggc
     3901 tggcggtggc ccgctggctg gaggcgcggc cggctgcgat gcgtacggct gcggcgcggc
     3961 gacggctggc tgtggcacgg ccaccgacgg cacggccacc gacggcaccg ctaccgacgg
     4021 cacgcccacg ggcgaggcag catcgacgtt ggcaggatcg agtggggaag ggttgtgctc
     4081 ggacgtcacc tggctcctcc ttggacgctc cagtttgccc gacccacacc gcggtggcgg
     4141 acgagaaccg ctgcgcactg tgacgacgct cagcgaaccc cgccattctg agatgcatct
     4201 cagagctacc ggccgatcgc gctcggcaac cctccatgct ggagacacgc aatctgcccc
     4261 ggccccaggc cgaagcagaa ggaggctaca tgtacggcac cgactattcc gataccagct
     4321 ggatgagcca aggcaactgt tcgcatcgcg acccggcgat gttcttcccg tccgatggag
     4381 tgggcgtcga aatcgcccga aaggcatgcc agggatgccc ggtcattgcc gagtgcttgg
     4441 agtacgccat ctccgaacgc atcgatcacg gcgtgtgggg cggatgctcc gaacgtgaac
     4501 gacgccgcat catccgggcc cgcgcccagg ccgccaaggc tcagccggtc accatcggct
     4561 gagcccggca ccgctgactc accggggcgc tccgctgggc cctccgggcg cgacaacggc
     4621 gtcccgacca tgccgtgcgg caaacggggc cgtgcggcaa tcgggacgcc ggcgaacggc
     4681 gttggattac gacgacgccc tgtcagcctt gtcgtcggat tcctcggagg ctccctcgtc
     4741 caagccctgc ttgaactcct tcttcgcttc gccgagcgag cgggcaagct tggggatctg
     4801 ggagccgccg aacaacacga cgacggcgat gatgatgaaa atccactcgg aaccctgagg
     4861 catgccctca ccctaccccc gaagttccgt cgtgcgacat cgccccgttc gagtgtgaac
     4921 tatgagcggg cggcgatgag gggttagacc ttccacatgg cctggcccag ccccgattcg
     4981 ccgaactccg aacgcgacgc gcaaccgacc gcaacggatc ttcgaatgac gatgcgccga
     5041 cgtcgccgcg aactcgatga cgccgagcag cattccgctg cggaggcgat cgcggtgcac
     5101 ctgttgagcg atcgacgggt cagcgaggcc cgccgggtgg gcgtgtatct ggccttcgac
     5161 ggcgaggtgc gcctggatca ggcgatcgaa gcgctgcagc gccgaggaat tgaggtggcc
     5221 gccgcagtcc tcggcgacgg tcggttgcgg ttcgcctcga tcggcccgga tacgccgtgg
     5281 cgcaccaatc gttacggcat cgacgaaccc gatattccgg ctgcgctcac cctcgacggc
     5341 ggcgaaatcg acgtggtcgt ggtgccagcg gtcgcggtcg atcttcgggg aaatcgcgtt
     5401 gggatgggtg ccggctggta cgaccgcacc ttcgccgggc gcagcggcga gtcgccgctc
     5461 ctggtcgcca cggtgcacga ttttcaggtg gtggcaggta tcgccagcca accccacgac
     5521 gtggccgtgg acgcgatcgt cacgccgacc agatggattg acgcgacgct cggttgaacc
     5581 gcggccgaca cgggcgg
//
LOCUS       DAOOMA010000097         5571 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_17480_length_5571_cov_3.728064, whole genome shotgun sequence.
ACCESSION   DAOOMA010000097 DAOOMA010000000
VERSION     DAOOMA010000097.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 5571)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5571)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5571
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17480_length_5571_cov_3.728064"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(56..1906)
                     /gene="dxs"
                     /locus_tag="PLP95_04040"
     CDS             complement(56..1906)
                     /gene="dxs"
                     /locus_tag="PLP95_04040"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HPB45003.1"
                     /translation="MLLDTIDGPADLRRLDAASLATLCSEIRELVVETATRVGGHLGS
                     NLGAVELTIALHRAFDFPNDILLWDTGHQAYVHKIVTGRRAEFANIRQPGGISGYPSR
                     AESPHDWIENSHASTVLSYAHGLATAQHLSGDPMRHIVAVIGDGSMTGGMAYEGLNNL
                     GHSGRKVIIVLNDNGRSYAPTVSRLGESLSRLRSNPVYRRQQARVERMLGDLPMVGGY
                     IEKGVGGAKAAVREMLEPPAFFEDLGVKYLGPFDGHDIAGLEKAFANATEIDGPVVLH
                     VRTQKGRGYAPAENDTIKHMHDMGAVKDGSYTAAFRDAMLEQGAKRPELVAITAAMPD
                     STGLLPFGEKFPERLIDVGIAEQHAVTAAAGMAMGGLRPVFAVYSTFLTRAFDQVNLD
                     VGLHGQPVVFVLDRAGITGDDGASHHGVLDMVLLTKVPNMTVFAPSSAAEVGRMLEDA
                     FELCTDGPAAIRFPKTMPPEGEDVAVGSGLNARRVRSGDEICIIGVGKMLSRSVEAAD
                     LLARDGHEVTVWDPRVVKPLDADMLTDAARHRLVITIEDGLRDGGVGSAIADALRDLA
                     PTGGPSVRVLGVPSAYLPHDKPDSLLANLGLTSEGILSEIAAWQRASADH"
     gene            complement(1946..2137)
                     /locus_tag="PLP95_04045"
     CDS             complement(1946..2137)
                     /locus_tag="PLP95_04045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB45004.1"
                     /translation="MHHPGLTFYLITIVTSSLGAPSFAMGHRVGDDDEALAEAEAEVL
                     SHHSHHSHRHDVAPPQAGT"
     gene            complement(2119..3024)
                     /locus_tag="PLP95_04050"
     CDS             complement(2119..3024)
                     /locus_tag="PLP95_04050"
                     /inference="COORDINATES: protein motif:HMM:TIGR00374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HPB45005.1"
                     /translation="MRIEPELTPPRRSILHSVPLRILLSAVMIAVLIWRLPDVKWGDL
                     GPRWTRASPLWMAGAGLAMLSAFALSTLRWQRVLAAMTTPPSYKRMLNIFLAGQFVSN
                     VLPSAFGGDVIRVARLGKRLEDRPTAFASVTIDRLTGWFVLPIITLVALSYQRELRDL
                     GHQTTVAVAIAVGTLVALSAVLLIASNRRFAKFADDAVGWRRFLIAIHVGIDALRRRP
                     AEAIEVIAIGLAFQFLQCLALWFAGHGIGIKEIEFGVVLVFFPAVAIAQNLPIGLGGL
                     GIRESMFVSFFGAVGAEPALCITLG"
     gene            3099..3956
                     /locus_tag="PLP95_04055"
     CDS             3099..3956
                     /locus_tag="PLP95_04055"
                     /inference="COORDINATES: protein motif:HMM:NF012770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HPB45006.1"
                     /translation="MEQRQVSLQAGEFVFLEAGAGGRPLMLVHGFTGSKEDFADELDR
                     LAADGFHAIAPELCGHGDSVWFDDESAYSLEAYTADMWELVDRLGWQRFSLLGHSMGG
                     MIAQMMAISAPERLEALVLMDTHHGSITGIDEELLDAALDVVRHQGLGVLHELLKFGA
                     DPAQNPAFERTCAERPGYREWAENKMLNTSPAMYAAMIRQLPTVVDRLDELEGLFVPT
                     LVMVGELDVGFVEPSKRLSERIPNARLDVLADGGHCPQFEATEAWRASLSEFLTAVVS
                     GTDAVVVDL"
     gene            complement(3963..4283)
                     /locus_tag="PLP95_04060"
     CDS             complement(3963..4283)
                     /locus_tag="PLP95_04060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB45007.1"
                     /translation="MTLAGAICLTIGIGAMVIESAGLGGGHGWIATTIGIVAAVAGLA
                     VCTPASRPSSPEATWVGLSPYRARSTGPCPVSVDANPHLPTPPERIKVVAWRDTPALP
                     AVRS"
     gene            complement(4419..4796)
                     /locus_tag="PLP95_04065"
     CDS             complement(4419..4796)
                     /locus_tag="PLP95_04065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hotdog domain-containing protein"
                     /protein_id="HPB45008.1"
                     /translation="MSLDTGLEATIELVISDADTAVAMRSGSVEVLATPRLIALFEQT
                     CVAALGDRVDAGSTTVGLSVQVDHLRPVGVGDTIAAEAHLDKIDGRRLHFSVAAKDSR
                     GLIAAGRITRVVVEVDHFMEKCR"
     gene            4868..>5571
                     /locus_tag="PLP95_04070"
     CDS             4868..>5571
                     /locus_tag="PLP95_04070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012231498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase modulator"
                     /protein_id="HPB45009.1"
                     /translation="MIDETTLQRVLAVAASGGADFAEVFAEDKRSSSAVLDDGKVEEL
                     TSGRDRGAGIRVVVGESTGFAHTADLSEASLLETARIAAAAARGSGGGTTVAELARVD
                     VARPNVVAILPGDVAKARKVELLTRANEAARSQGAAITQVMARYSDSRRRILVANTDG
                     VLGEDDQVKTVFTVGTVASGDTGMQTGRQTVGHTVGFELFDQIDVEEMGRLAALQAIT
                     KLAARPAPSGQMTVVIG"
BASE COUNT         1006 a         1706 c         1931 g          928 t
ORIGIN      
        1 cgacgcgacc gaccacgcag caggagagaa tcccttctac cactgacgcc gaggctcagt
       61 ggtcggcgct ggcgcgctgc catgcagcga tttcgctcag aatgccttcc gaggtcaggc
      121 cgagattcgc cagcagcgag tcgggcttgt cgtgagggag ataggcggag gggacgccga
      181 ggactcgaac cgatgggccg ccggtggggg caagatcgcg tagggcatcc gcgattgcgg
      241 atcccacacc gccgtcgcgc aatccgtctt cgatcgtgat gacgagacgg tggcgtgcgg
      301 cgtcggtcaa catgtcggcg tcgagcggct tgacgacacg ggggtcccag acggtgacct
      361 catggccgtc gcgggcgagc agatcggcag cctcgacgct tcgagacaac attttgccga
      421 ccccgatgat gcagatctcg tcaccggaac ggacacggcg agcgttcaga ccgcttccga
      481 ccgccacgtc ctcgccttcg gggggcatcg tcttggggaa acgaatcgcg gccgggccgt
      541 cggtgcacaa ttcgaatgcg tcttcgagca tccggccgac ctcggcggcg gaggacggag
      601 cgaacaccgt catgttgggc accttggtga gcagcaccat gtccaacaca ccgtggtgac
      661 tcgcgccgtc gtctccggtg atgccggcgc ggtcgagaac gaacaccacg ggttgaccgt
      721 ggaggccgac gtctagattc acctgatcga aggctctggt gagaaacgtc gagtacacgg
      781 cgaacaccgg ccgcagcccg cccatcgcca tgccggcagc agcggtcacc gcgtgctgtt
      841 cggcgatgcc gacgtcgatg aggcgttcgg ggaacttctc gccgaagggc aacaggccgg
      901 tcgagtcagg catcgccgcg gtgatggcga cgagttcggg acgcttcgcg ccctgctcca
      961 acatcgcgtc gcgaaatgca gccgtatagg agccgtcttt caccgcgccc atatcgtgca
     1021 tgtgcttgat cgtgtcgttc tcggccgggg cgtaaccgcg ccctttctgg gtgcgcacgt
     1081 gcaacacgac ggggccgtcg atttcggtgg cgttggcgaa cgccttttcg agaccagcga
     1141 tgtcgtggcc gtcgaagggc ccgaggtact tgaccccgag gtcctcgaag aacgccggcg
     1201 gttcgagcat ttcgcggacg gccgccttgg cgccgccgac gcccttttcg atgtatccgc
     1261 cgaccatggg gagatcgccg agcatccgct caacccgggc ctgctggcgc cggtacacgg
     1321 ggttcgatcg gagccgcgac agcgactcgc cgaggcgtga cacggtcggc gcgtagctgc
     1381 gcccgttgtc gttgagcacg atgatgacct tgcggcccga atgcccgaga ttgttcaagc
     1441 cctcataggc cataccgccg gtcatcgagc catcgccgat gaccgcgacg atgtgtcgca
     1501 tcgggtcgcc ggagaggtgt tgtgccgtcg ccagtccgtg ggcgtaggac aacaccgtcg
     1561 acgcgtggga gttctcgatc cagtcgtgtg gcgactcggc tcgcgacggg tatccggaga
     1621 ttccgccggg ttgacgaatg ttcgcaaact cggcccgtcg gccggtcacg atcttgtgga
     1681 cgtacgcctg atggccggta tcccacaaca agatgtcgtt cgggaagtcg aaggctcggt
     1741 gtagggcaat ggtcaactcg acggctccga ggttggagcc gaggtgcccg ccgacccggg
     1801 tcgccgtctc gacgacgagt tcccggattt cggaacagag tgtggcgagt gacgcagcat
     1861 cgagacggcg aagatcggcc ggcccgtcga tcgtgtcgag aagcatcagg agaggaatcc
     1921 tccgcgttga ggcagtggaa tcaatctagg tcccggcctg aggcggtgcg acatcgtggc
     1981 ggtgtgaatg atgtgaatga tgcgagagca cctcggcctc cgcctcggcg agcgcctcgt
     2041 cgtcgtcgcc cacccggtgg cccatcgcga acgacggggc gccgagggaa ctggtgacga
     2101 tggtgatgag gtagaaggtc agcccagggt gatgcacaac gccggttcgg ctccgaccgc
     2161 accgaagaac gacacgaaca tcgactcccg gattccgaga ccgccgaggc cgatcggcag
     2221 attctgtgcg atcgcgacgg cggggaagaa caccagcacc acgccgaatt cgatctcctt
     2281 gattccgatg ccgtggccgg cgaaccacaa cgccaggcat tggaggaact gaaacgccag
     2341 accgatggcg atgacctcga tcgcctctgc tggccggcgt cgaagggcgt cgatgccgac
     2401 gtggatggcg atcaggaacc gtcgccatcc gacggcgtcg tcggcgaact tcgcgaaccg
     2461 tcggttggaa gcgatcaaca ggaccgcgct gagcgcgacc agggtgccca cggcgatagc
     2521 caccgcgacc gtcgtctggt gcccgaggtc gcgtaactcc cgctgatacg aaagtgccac
     2581 cagggtgatg atgggcaaca cgaaccaacc ggtcaggcgg tcgatcgtga ccgaagcgaa
     2641 cgccgtcggt cggtcttcga gccgcttgcc gagccgcgcc actcggatca cgtcgccgcc
     2701 gaatgccgac ggcaagacgt tggagacgaa ctgtccggcg aggaaaatat tgagcatccg
     2761 cttgtacgac ggtggtgtcg tcatcgctgc gagcacccgt tgccagcgaa gtgtcgacaa
     2821 cgcgaacgcg ctcaacatcg ccagcccggc acccgccatc cacagcggcg aagcgcgggt
     2881 ccatcgcggc ccgaggtccc cccacttcac atcgggaaga cgccagatga gcacggcgat
     2941 catgaccgcg ctgagcagga ttcgaagcgg gacgctgtga agtatcgacc gccgtggggg
     3001 agtgagctca ggttcgatcc gcacagtatt tcatctgggt cgacgcttcg gggtgtaccg
     3061 cggtgcggac tcggcgaccg gcggtacggt gtgcggtcat ggaacagcga caggtgagcc
     3121 tgcaagcggg ggagttcgtg tttctggagg caggtgccgg tgggcgcccg ttgatgttgg
     3181 tgcacggatt caccggctcc aaggaagact tcgccgacga actcgatcgg ctggccgccg
     3241 acgggtttca cgcgatcgcc cccgaactct gcggccacgg tgacagcgtc tggttcgacg
     3301 acgagtcggc gtactcgctc gaggcgtata cggcggacat gtgggagctc gtcgaccgtc
     3361 tcggatggca acggttctcg ctgctcggcc attcgatggg cggcatgatc gcgcagatga
     3421 tggccatctc cgcgcccgaa cgtcttgagg cgctcgtgtt gatggacaca caccacggga
     3481 gcatcaccgg catcgatgag gagttgctcg atgctgcgct cgacgtggtg cgtcatcagg
     3541 ggctgggcgt gctccacgaa ctgctcaagt tcggcgccga cccggcccag aacccggcgt
     3601 tcgaacggac ctgcgccgag agaccgggct atcgggagtg ggccgagaac aagatgttga
     3661 atacgtcgcc tgcgatgtac gcggcgatga ttcggcaact accgacggtg gtggatcggc
     3721 tcgatgaact cgaggggttg ttcgtcccga ccctggtcat ggtcggcgaa ctcgacgtcg
     3781 ggtttgtgga accgtcgaag cgactctccg agcgaatccc gaacgcgcgc ctcgatgtgt
     3841 tggccgacgg cggtcattgc ccacaattcg aggcgaccga ggcgtggcgc gcttcgttgt
     3901 cggagttcct gacagcggtg gtgagcggaa cggacgccgt tgtcgtcgat ctgtgagcga
     3961 gcctacgagc gaacggcggg tagcgccggg gtgtcgcgcc aagccacgac tttgatccgc
     4021 tcgggagggg ttgggaggtg cgggttggca tcgaccgaaa ccgggcacgg acccgttgag
     4081 cgggcacgat agggcgaaag ccccacccac gttgcctctg gcgaggatgg tcggctggcc
     4141 ggggtgcaca ccgccaggcc tgccaccgca gcgacgatac cgatcgtcgt cgcgatccag
     4201 ccgtgcccgc cgccgagtcc ggccgactcg atcaccatcg caccgatacc gattgtcagg
     4261 cagatggcac cggccagtgt catgcaccac gcggccacct cgactgccga gcgggttccg
     4321 gtggggtgag tcgtatggcg agtgggggag ttcacctgcc gggtatcgac gtggtccgtt
     4381 gtccacctga gaaatctccg gcgaacgtca acccgtcgct accggcactt ctccatgaaa
     4441 tggtcgactt cgacgaccac ccgggtgatc cgtcctgcgg cgatcaggcc ccgggaatcc
     4501 ttcgcagcga cgctgaaatg cagacgtcgt ccgtcgatct tgtcgaggtg tgcttcggcg
     4561 gcgatcgtat cgccgactcc aaccggtcga aggtggtcga cctgcaccga aagcccgacg
     4621 gtggtgctgc cggcatcgac ccggtcgccg agcgcggcca cgcaagtctg ttcgaaaagc
     4681 gcgatcagtc gtggggtggc gaggacctcg acgctgcccg agcgcatcgc gacagcggtg
     4741 tccgcgtcgg aaatcaccag ttcgatggtg gcctcgaggc cggtgtccaa actcacgctc
     4801 gataccataa ggagtcgaac ggcgaagggc caagtgcctg cctcgaaatg ctcgaacgga
     4861 gaggtcgatg atcgacgaga caaccctgca gcgtgtgctc gcagtcgctg caagcggagg
     4921 tgctgatttc gcggaggtgt tcgccgaaga caagcgatcc tcgagcgcgg tgctcgacga
     4981 cggcaaggtt gaggagctga cctcagggcg cgaccgaggg gcgggaatcc gggtggtggt
     5041 gggcgaatca accggctttg cccacaccgc ggacctgtcg gaggcgtcgc tgttggaaac
     5101 cgcgagaatc gccgccgcag cggcacgggg gtccggcggc gggacaacgg tggccgagtt
     5161 ggcccgggtc gacgtggcgc gacccaatgt cgtggcgata ctgcccggcg atgtggccaa
     5221 ggcgcgaaag gtcgagttgc tgacccgggc gaacgaggca gcgcgttcgc agggcgctgc
     5281 gatcacgcag gtgatggcgc ggtactcgga ttcgcgacgt cgaatcctgg tggccaacac
     5341 cgacggcgtc ctcggcgagg acgatcaggt gaagaccgtg ttcaccgtcg gtaccgtcgc
     5401 gtctggggac accgggatgc agaccggtcg ccagaccgtg ggccacacgg tcggcttcga
     5461 actgttcgat cagatcgatg ttgaggagat ggggcgtcta gcggccctac aggcgattac
     5521 caaactcgcc gcccgcccgg cgccgagtgg tcagatgacc gtcgtgatcg g
//
LOCUS       DAOOMA010000098         5555 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_17562_length_5555_cov_3.374909, whole genome shotgun sequence.
ACCESSION   DAOOMA010000098 DAOOMA010000000
VERSION     DAOOMA010000098.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 5555)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5555)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5555
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17562_length_5555_cov_3.374909"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..494
                     /locus_tag="PLP95_04075"
     CDS             <1..494
                     /locus_tag="PLP95_04075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB45010.1"
                     /translation="RGMLLDTSRPADALDSVKVAEWKGGRAPGERYQLMPVDTVGVAA
                     DESFELAEPGPEPWPGSLPDPPPALVWSQPRPAEVVDDAGALVRIDRRGRRSGDPARL
                     SIESGPWQPVVAWSGPWLVDERWWDAQGHRRRARFQVCTEAGAAHLVTLEAQRWWVEA
                     TYD"
     gene            583..1263
                     /locus_tag="PLP95_04080"
     CDS             583..1263
                     /locus_tag="PLP95_04080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012227890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HPB45011.1"
                     /translation="MKVLVIEDEVALADAVARGLGAEGFEVTVANDGLEGLELARGAK
                     FDLIVLDIMMPGMNGYRVCRTLREEEIWTPILMLTAKDGEYDEAEALDTGADDFLSKP
                     FSFVVLVARLRALARRRSDANLAPLEFDDLTLEPVSRTVRRGDSDISLSPREFALLEA
                     LMRHSGEVVSKTDLLDRVWGEDFEGDPNVVEVYIGYLRRKIDRPFGRSDLETVRGEGY
                     RLGSPLPK"
     gene            1421..2893
                     /locus_tag="PLP95_04085"
     CDS             1421..2893
                     /locus_tag="PLP95_04085"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPB45012.1"
                     /translation="MSNRWASRVQRRLAYLAPRRLFGSVRVRLTFAVSVIFAGALLLA
                     SAGLIRQVESALVSDARARNDAMVHTLGKMVASGDLDALAAQDISQLLNGISDSAQAE
                     ILREGLNNSFVYVQGAGSAQISQDMSVLERIKRVVTDEEVPLWGKVLPSRIDEQQYVV
                     SRVEIPTPGGNIVLSVARSLEEVKRTVDQVRGALTFAVPSLIAAVALSAWMMTGQVLR
                     PVQAITRRAREITATTLDQRVPVPQTDDEIGQLARTMNAMLDRLQEASESQKRFVSDA
                     SHELRSPVASIRVQLETALMCPDDTDWEKVGRTVLAEDVRLSALVDNLLALARIEEGQ
                     RRHLGEVNVDELIHEQLSRQRRVTIDRSGVLAGRVYGVRDELMSVVRNLIDNGERHAQ
                     ERMRVSLVTKGPWVRLCVEDDGPGVDPALREKVFERFARLQEARNRDAGGAGIGLALT
                     KRVVEAHSGRVFVEDSDLGGASFVVELPSADFDPNDEFAD"
     gene            complement(3014..3862)
                     /locus_tag="PLP95_04090"
     CDS             complement(3014..3862)
                     /locus_tag="PLP95_04090"
                     /inference="COORDINATES: protein motif:HMM:NF024221.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HPB45013.1"
                     /translation="MSNPLLNDKHFNDPAQRSDTAARGTIDAQWNQSYDQLAHGTQAA
                     TTTGAMTMGGTVSATLVLFAFAIATAVYGWSRVSSEFLGFDATGARVYGPVGSMGWLF
                     GPMIVGFGLAMVTSFKPHLAKVLAIPYALAQGLLLGFISHYYNIAYPGIAVTAVLATA
                     GVFAAMLVLYGFRVLRVTPRMTKAVIAATFGVLVVYLVNFVVGLFTSGPSMLAGSGPI
                     SIAVSVIVVGIAAFNLLLDFDFIEKGVANRLPKEYEWFAAFGLMVTIIWLYLELLRLL
                     SKLRDN"
     gene            complement(3964..5217)
                     /locus_tag="PLP95_04095"
     CDS             complement(3964..5217)
                     /locus_tag="PLP95_04095"
                     /inference="COORDINATES: protein motif:HMM:NF012296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HPB45014.1"
                     /translation="MTARTGTAVEPTDEPAGEPVRALTGIDLLAPTWGRGVPHDQFDR
                     LRREAPVYRHHEPDGPGFWAITKHADVRRVSHDWKTFSSELGGTFIPTADEETLAQLR
                     LTILNMDPPKHHRARRLISKAFTPRMISKLTEDIEARAELVIDEVIERGECEFVEEIA
                     AQVPVQMICEMIGLERELWPRMFEISNDLIGSRDDPEYAHVNSNEASAEIFMLCDAVA
                     ADRRVTPRDDLMTALVHAEIDGERLDNTDLNLFFVTLVVAGNETTRNLINHSLLALLD
                     HPEQAEQLRSQPELWQSAVEEMLRWGSSIHNFRRTATRDTEIRGMAIAAGDKVVLYYA
                     SANRDEEVFEDPHRFDITRTPNDHVAFGGGGVHYCLGASLARAEIRATMRQVVERLVN
                     IELTSAPERLKNDFVNGIKRMHVRW"
     gene            5338..>5555
                     /locus_tag="PLP95_04100"
     CDS             5338..>5555
                     /locus_tag="PLP95_04100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB45015.1"
                     /translation="MGFNNRAMSWKEFESALSSRLPDSIQASHRGEPHPGDGSDSPAW
                     SRHRLPYEANAVRATATVPYAELHAHSN"
BASE COUNT          904 a         1717 c         1930 g         1004 t
ORIGIN      
        1 tgcgcggcat gctgctcgac acgtcgcgtc cggccgacgc gctcgactcg gtcaaggtcg
       61 ccgagtggaa gggaggtcgg gcgccggggg agcggtatca actcatgccg gtcgacacgg
      121 tcggggtcgc ggccgacgag tccttcgaac tcgccgagcc cggtccggag ccctggccgg
      181 ggagcctgcc cgacccgccg ccggcgctcg tgtggtctca gccgagaccg gccgaggtgg
      241 tcgacgacgc cggcgcgctc gtgcgtatcg accgtcgagg gcgacgaagc ggcgacccgg
      301 ctcggttgtc gatcgagtcg gggccctggc aaccggtggt ggcctggtcg gggccgtggc
      361 tggtcgacga gcggtggtgg gacgcacagg ggcatcgccg gcgtgcccgc tttcaggtgt
      421 gcaccgaggc cggcgcagcc catctggtca ccctcgaggc gcagcgatgg tgggtcgaag
      481 ccacctacga ctgagtgggt cgacttcgct cggtcagggc tgctgtgagg tgcctcatcg
      541 ctttggaacg attctcggcg gttcatccga cagaatggac ccgtgaaggt cttggtcatc
      601 gaagatgagg tcgcactcgc ggatgccgtc gcccgtgggc tcggcgctga aggattcgag
      661 gtcaccgtcg ccaacgacgg cctcgagggt ctcgagttgg cgcgtggggc aaagttcgac
      721 ctgatcgtgc tcgacatcat gatgccgggt atgaacgggt accgggtgtg tcggaccttg
      781 cgggaggagg agatctggac tccgatcctc atgctcaccg ccaaggacgg ggaatacgac
      841 gaggccgagg cgctcgacac cggggcggac gactttttgt ccaagccgtt cagcttcgtg
      901 gtgctcgtcg cgaggcttcg tgcgttggcc cggcgtcgca gcgacgccaa ccttgctccg
      961 ctcgagttcg acgatctcac cctcgaaccg gtgtctcgca ccgtgcgtcg cggcgacagc
     1021 gacatctcgt tgtcaccccg cgagttcgcg ctgctcgagg cgctcatgcg ccacagcggt
     1081 gaggttgtga gcaaaaccga cctcctcgat cgggtgtggg gcgaagactt cgaaggcgac
     1141 ccgaacgtcg tcgaggtgta catcgggtat ctgcgccgca agatcgaccg cccctttggc
     1201 cgatccgatc tcgaaaccgt tcgcggtgag ggctaccggc tcggctcacc gctgcccaag
     1261 tagcgcggag ccgaccgtga ccgatacggg cgagacgggt ggcgctgcag tctcggcagg
     1321 gccgtctgta gcctcggtcg ggccgcctgc tcatccgcct gctgcgccac ctgctgtgcc
     1381 agccgccggc gaggctccgc cgcctccgaa ggcgccactg ttgtcgaacc gttgggcctc
     1441 acgggtccag cgtcgtttgg cgtatctggc cccgcgtcgc ctcttcggtt cggtccgcgt
     1501 ccggttgacc ttcgcggtgt cggtgatctt cgccggagcg ttgttgctgg cctcggccgg
     1561 tctgatccgc caggttgaat ccgcgctggt cagcgacgct cgggcgcgca acgacgccat
     1621 ggttcacacg ttgggaaaaa tggtggccag cggagacctc gatgcgttgg ccgcccagga
     1681 catctcccaa cttctgaacg gcatttcgga ttcggcacag gccgagatcc tgcgcgaggg
     1741 actcaacaac agcttcgtct acgtgcaggg cgccggaagc gcacagattt cgcaggacat
     1801 gtcggtgctc gaacggatca agcgagtcgt gaccgacgag gaggtgccgc tgtggggcaa
     1861 agtgcttccg agccgcatcg atgaacagca atacgtcgtg agtcgtgtgg aaatcccgac
     1921 gcccggtggc aacatcgtgt tgagtgtcgc ccggtccctt gaggaggtga aacgcaccgt
     1981 cgatcaggtt cgcggggcac tcaccttcgc cgtgccctcg ttgatcgccg cggtcgcgct
     2041 ctccgcgtgg atgatgaccg gccaggtgtt gcgtccggtc caggcgatca ctcgccgtgc
     2101 ccgggagatc acggcgacga ccctcgatca gcgggtcccc gtgcctcaga ccgacgacga
     2161 gatcggccag ctcgcccgca cgatgaacgc catgttggat cggctccaag aagcctcgga
     2221 gtctcagaag cgattcgtgt ccgacgccag ccacgagttg cgatcgccgg ttgcatcgat
     2281 tcgggtacaa ctcgaaacag cgctcatgtg ccccgacgac accgattggg agaaggtggg
     2341 acgcacggtg ctggccgagg acgttcgcct ctcggcgctc gtcgacaatc tgttggcact
     2401 cgcccgcatc gaagaggggc agcgccgtca cctcggcgag gtcaacgtcg acgaactcat
     2461 ccacgagcag ttgtcgcggc agcgccgggt gacgatcgat cgttccgggg tgctcgccgg
     2521 acgcgtctat ggggtgcgcg atgaactgat gagcgtggtg cgcaacctca tcgacaacgg
     2581 cgaacgccat gcccaggaac gcatgcgggt ctcgctggtc accaaggggc catgggtgcg
     2641 gttgtgcgtc gaagacgacg gcccaggggt cgaccctgcg ttgcgcgaga aggtgtttga
     2701 gcgcttcgcc cgcctgcagg aggcccgcaa ccgcgatgcg ggcggggctg ggatcgggct
     2761 ggcgctcacc aagcgggtcg tcgaggccca cagcggccgg gtcttcgtcg aagattccga
     2821 cctcggcggc gcatcgttcg tggtggagtt gccgtcggcc gactttgacc cgaacgacga
     2881 gttcgccgac tgaccccgac tgaccccgac tgcccgcccc cgcccgcttt acagttttgt
     2941 aggtactttc tgcggtcctg gccgcagaaa gtacctacaa aacgccgaat gtgggggccg
     3001 gtggggcgtc gacctagttg tcgcggagct tggacagcag gcgcagcaac tcgaggtaca
     3061 gccagatgat ggtgaccatc aaaccgaagg cggcgaacca ctcgtattcc ttcggcaggc
     3121 ggttggcgac gcccttttcg atgaagtcga agtccaacaa caggttgaac gcggcgatcc
     3181 cgaccacgat gacgctgacc gcgatgctga tcggccccga tccggcgagc atggacggcc
     3241 cgctggtgaa gagcccgacg acgaagttca ccaggtagac gacgagcact ccgaaggtgg
     3301 cggcgatgac cgccttggtc atgcgaggcg tgacgcgcag cacccggaat ccgtacagca
     3361 ccaacatggc ggcgaacacg ccggcggtgg ccagcacggc ggttaccgca atgccggggt
     3421 aggcgatgtt gtagtagtgc gagatgaacc cgagcagcag accctgggcg agggcgtagg
     3481 ggatggcgag caccttggcg aggtgcggtt tgaacgacgt gaccatcgcc aggccgaagc
     3541 cgacgatcat cgggccgaac agccagccca tcgacccgac cgggccgtag acccgtgcgc
     3601 cggtggcgtc gaagccgaga aattcgctgc tcacccgcga ccagccgtag acggcggtgg
     3661 cgatggcgaa tgcgaacagc accaacgttg cgctgacggt gccgcccatc gtcatcgccc
     3721 cggtggtggt ggcggcctgg gtgccgtggg cgagctggtc atagctctgg ttccactggg
     3781 catcgatcgt gccgcgcgcg gcggtgtcgc tgcgttgggc cggatcgttg aagtgtttgt
     3841 cattgagtag cgggttgctc acggagttac ctcgtctcgg gaatggggca aggttaccgg
     3901 ccggcggggg tgtgccggtg gcgctgcttg agaggtcgcg tggacggccg ggtgccgccc
     3961 cggtcaccaa cgtacgtgca tgcgtttgat gccgttcacg aagtcgtttt tgagtcgttc
     4021 gggggcactg gtgagttcga tgttcacaag ccgttcgacg acttgtctca tggtggcgcg
     4081 aatctctgcc ctcgccaggc tcgccccgag gcagtagtgc acaccgcctc cgccgaatgc
     4141 gacgtgatcg ttcggcgtgc gggtgatgtc gaagcgatgc ggatcctcga agacctcctc
     4201 gtcgcggttc gccgaggcgt aatagaggac caccttgtcg cccgcagcga tcgccatccc
     4261 gcggatctcg gtgtcgcggg tggcggtgcg gcggaagttg tggatcgacg aaccccaccg
     4321 cagcatttcc tccacggcgc tttgccacag ctcgggttgg ctgcgcagct gttcggcttg
     4381 ttcggggtga tcgagcagag cgagaagtga gtggttgatc agattgcggg tggtctcgtt
     4441 gccggcgacc acgagggtga cgaagaacag gttgaggtcg gtgttgtcga gccgctctcc
     4501 gtcgatttcc gcgtggacca gggcggtcat gaggtcgtcg cggggagtga cccggcgatc
     4561 ggcggctacg gcatcgcaca acatgaagat ctcggcggag gcctcattgg aattcacgtg
     4621 tgcgtactcg gggtcgtcgc gcgagccgat gaggtcgttg gagatctcga acatgcgggg
     4681 ccacagctcg cgttcgagcc cgatcatctc gcagatcatc tgcaccggga cctgggcggc
     4741 gatctcctcg acgaattcgc actcaccccg ctcgatcacc tcgtcgatga ccagctcggc
     4801 gcgcgcctcg atgtcctcgg tgagtttcga gatcatgcgc ggggtgaagg ccttcgagat
     4861 caaccggcgc gcacggtggt gtttcggcgg gtccatgttg aggatggtga gacgcagttg
     4921 tgcgagggtt tcctcgtcgg cagtggggat gaaggtgccg ccgagttcac tggaaaaggt
     4981 cttccagtcg tggctgaccc gtcgcacgtc cgcgtgtttg gtgatcgccc agaagccggg
     5041 gccgtccggt tcgtgatgtc gatacacggg cgcctcgcgg cggagccgat cgaactggtc
     5101 gtggggaacg ccccgacccc acgtcggggc cagcaggtcg ataccggtca gcgccctaac
     5161 cggctcgccg gctggttcat cggttggttc gacggccgtt ccggtcctcg cagtcacatc
     5221 caccccatta ccttgtgttc ttcgacctct cgtggtcgcc ccggccactg gctatcacag
     5281 atccgggcca cggacgcacc gccgcaagcg aacgaatgtt cgtctatggt ggggcggatg
     5341 gggttcaaca acagggcgat gtcgtggaag gagttcgaat cggcgttgag tagccgactg
     5401 cccgactcga ttcaggccag ccatcgcggc gaaccccacc ccggcgacgg ttccgacagc
     5461 ccggcgtggt cgcgccaccg tctgccctat gaggccaacg ccgtgcgggc gacggcgacg
     5521 gtgccctatg ccgagttgca tgcccattcc aattt
//
LOCUS       DAOOMA010000099         5545 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_17596_length_5545_cov_4.419854, whole genome shotgun sequence.
ACCESSION   DAOOMA010000099 DAOOMA010000000
VERSION     DAOOMA010000099.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 5545)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5545)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5545
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17596_length_5545_cov_4.419854"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1285)
                     /locus_tag="PLP95_04105"
     CDS             complement(<1..1285)
                     /locus_tag="PLP95_04105"
                     /inference="COORDINATES: protein motif:HMM:NF040160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5719 family protein"
                     /protein_id="HPB45016.1"
                     /translation="MIRRVMIIVMSVVAIAAMNQFVEPEGIGQPSPNPVVGGETSGRG
                     SGSTWFCPFGTSTAGEAAAPTNSILITTEDRAVVAEVTMYRTAALEDGSTTVSETVNA
                     AANTVTEVRLDDRVDGGATVEISGGSATVAQKLVAPNRSDQADCVTEAAQQAYFAAAQ
                     TTTGSFAQLWLVNPFPTSLSVDVRVTIEGGVRIPGPVSGVIVPARSSRLLDLGTIPEV
                     AELREQFSIAVEGRSGRFVAGLAQTVAGNGLRATIGVQHPATNWLIPYSFTGSDFSEK
                     LWIFNPSSEDTAVIASVFPQGVATEMMPEPFTFDLPGRQYTLIDLTAEGRLPTTELRW
                     IHVESLSDVGIVVNSVVSITAASGTGEPNTRPALAGGLAAQVGATVQATSWIVSSVDP
                     ASESQSVVAVANPSPESIAVLTITRIARSGDGAPAE"
     gene            complement(1325..4591)
                     /locus_tag="PLP95_04110"
     CDS             complement(1325..4591)
                     /locus_tag="PLP95_04110"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HPB45017.1"
                     /translation="MSARRPASPTSGSRRRRSDTTRSDRPSSSSSVESPASADEEWNP
                     DATPFVEGYDAADEAESPVAGVGVPAVVAVIVTRNPGARFDDLLASISAQEYANLVTL
                     VVDAGSDSDPTERVAEVLPTAFVKRIDAGGFAVAANAALGTVDGASFYLVLHDDVELG
                     KGAVQAMVEEAFRSNAGIVGAKLLDGADRRTLTDVGASIDKFGFLWPYAEPGELDQSQ
                     HDAIREAFVVSGAAMLVRCDLFRDLGGFSTDIDGSGEELDLCWRARVAGARTVVMPAA
                     QVFHHAESTFDKAHERGLRLALRHQARIMLACYSALTLLRILPQAMVLGVLDLVGALL
                     RGRWRQAGDVFTAFMWNLGHLPRTLSIRRSTQKSRRTPDDEIRRLQIKGSARFTNFFH
                     AYTARDRSLGQAVAAAARGMSGPNDAHNTGIWGIGAFLIAAVVLVGGSRSLITGGVPV
                     VREFVTNSPSGDLLREWWSGWRSAGLGQSGGSPTVAFVVGALNWMTFGSEGLVRTVVF
                     LAPLGVGALGAWRLLRGVGSPLARTAVLVAYLANPIPYNAIAEGRWQALVVWALAPSI
                     VARLAAAGAFEPFADTERAERSVLQQTFALGLIVAIGATVAPVIVLVVALLAVVMAVV
                     VGAGSRGNLGRLGVVTLGSLAVSALVHLPWTISVLGGANRVEVLLGNAAGRGAPIGVS
                     EALVFDTGAHGTFLSGGLLVAIAAAVLITVGPVFRWTLFGLVSALVGFGLVALAGLLA
                     PSVALPVPEVLLVPAALGAALCVGVAAEAITTTLSGTAFGIRHVAAVLCAVGVLVAVL
                     PLLLDSLNGRWDAPTSDVDDALESLSTGAEIENFRTLWIGDADAVAQAGWRFGDGMKF
                     AVSTGVSSGLPDLFPPAKGRRSGAGTPSQRRSSRRDEPCRSLPCAVRYPLCGGAGAAR
                     AVALRANAVRDRCGDEGTASPAVRPVVDRCGPGYRGVREPGRASGLVDHRCRCRVGVE
                     RRGGAEPRRSGRAATGRHVGSAPDTAGWRTVGRPDVDAVGRVGRSLDDEGRRRRGRTR
                     FGERLGVDRADRHRWQRVVGVSNPADSGRDAPRAVARVGGGGGQSPRNAVVDRTCAPA
                     "
     gene            4759..5433
                     /locus_tag="PLP95_04115"
     CDS             4759..5433
                     /locus_tag="PLP95_04115"
                     /inference="COORDINATES: protein motif:HMM:NF024248.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HPB45018.1"
                     /translation="MAGALAPIDLEHMRQHRALGDPTRHAVFVHLREAERPVDVAELT
                     AKFGLNHNAIRQHLARLADAGLITDELRASTGPGRPAKLYRVVPGAAQRWGGTSPHET
                     LADMLLEMVRTGRSALEIGRDTGRRLAADNAPAGSAVEILDGVARRLGFEPCVELTER
                     GADIVLGRCPFVDPATAAPEVVCALHRGIAEGIAEATDTDDVIEDLVMRPPATAGCRI
                     RVASSN"
BASE COUNT         1041 a         1986 c         1753 g          765 t
ORIGIN      
        1 cctcggcggg agcgccgtcc cccgaacggg cgattcgtgt gatcgtcaat acggcgatcg
       61 actccggcga tgggttcgcc accgccacga ccgactggct ctccgaagcc gggtcgacgc
      121 tgctcacgat ccacgaggtc gcctgcaccg tcgccccgac ctgcgcagcg aggccaccgg
      181 cgagcgccgg gcgcgtattc ggctcgccgg tgccagaggc cgcggtgatc gacacgacgc
      241 tgttcaccac gatgccgacg tcggacagcg actcgacatg aatccagcgc aactcggtgg
      301 tcggaaggcg cccttcggcg gtgagatcga tgagcgtgta ctggcggccg gggagatcga
      361 acgtgaacgg ttcgggcatc atctcggtcg cgacgccctg gggaaacacc gacgcgatca
      421 ccgcggtgtc ctcactgctc gggttgaaga tccacagctt ctcgctgaag tccgagcccg
      481 tgaacgaata aggaatcagc cagttcgtcg ccgggtgctg caccccgatc gtcgcacgaa
      541 gcccgttgcc cgcgaccgtc tgggcgaggc ccgccacgaa gcgcccggag cggccttcca
      601 ccgcgatcga gaactgttca cgcagctctg cgacctcggg aatcgtgccg aggtccaaca
      661 ggcgcgacga tcgcgccggc acgatgaccc ccgacaccgg ccccggaatg cgcaccccgc
      721 cttcaatcgt gacgcggaca tcgacgctga gcgacgtggg aaacgggttg acgagccata
      781 actgggcgaa cgacccggtc gtcgtctgcg ccgcggcgaa atacgcttgc tgggctgcct
      841 cggtcacaca atcggcctga tcgcttcggt tgggtgccac gagcttctga gcgaccgtgg
      901 ccgaacctcc cgagatctcc accgtcgccc cgccgtcgac ccgatcgtcg aggcgcacct
      961 ccgtgacggt gttggccgca gcattcaccg tttcggacac ggtcgtcgac ccgtcctcga
     1021 gcgcagcggt gcgatacatc gtcacctcgg cgaccacggc tcggtcctcg gtcgtgatca
     1081 gaatcgagtt cgtcggcgcc gcagcctcgc cagccgttga ggttccgaac gggcagaacc
     1141 acgtcgaacc actgccacgc ccgctcgtct cgccgccgac gaccgggttc ggtgaaggct
     1201 gcccgatccc ctcgggttcg acgaactggt tcatcgccgc gatcgcgacg accgacatga
     1261 cgatgatcat cacccggcga atcacaagat ctcctcggag gcgagcagtt cggccatctc
     1321 cggatcatgc gggcgcgcac gtccgatcca ccaccgcatt ccgcggcgat tgaccaccac
     1381 caccgccaac gcgagcaact gcacgaggtg catcgcgacc tgagtcggcg gggttcgata
     1441 ctccaacgac gcgttgccac cggtgccggt cggcgcggtc cacgcccagt cgctcaccga
     1501 atcgagttcg acctcgtctc cgccgacctt catcgtccag cgatcgtcca acccgcccaa
     1561 cagcatcgac gtctggtcgg ccgacagttc gccatccagc cgtgtcgggg gccgacccga
     1621 cgtgtcgacc ggttgcagcc cgacccgacc gacgaggctc agccccacct cgtcgctcca
     1681 ctccgacgcg acatcgacat cggtggtcga ccaaaccgga agcgcggcca ggttctcgta
     1741 cacctcgata cccggggcca catcgatcga cgacaggtcg aactgctggc gaagccgttc
     1801 cttcatcacc gcatcgatct cgtacagcgt tcgcccgtag ggcaacggcg cgagccgctc
     1861 ctgcaccacc acatagcgga taccgaactg cgcaagggag gctccgacac ggctcgtctc
     1921 gccgcgacga acgtcgctga gaaggcgtgc cagctccgct tctccggccc ttcgcaggcg
     1981 ggaacagatc cggcaacccc gacgacacgc cggtcgacac cgcgaacttc atgccgtcgc
     2041 cgaacctcca acccgcttgg gcaacagcgt ccgcgtcgcc gatccacaag gtccggaagt
     2101 tctcgatctc ggccccggtg ctcaacgact cgagcgcatc gtcgacgtcg ctcgtcggcg
     2161 catcccatcg accgttgagc gaatcgagca acaacggcag gaccgcaacc agaaccccga
     2221 cggcgcacag aaccgccgcg acgtggcgga tgccgaaggc ggttcccgac agggttgtcg
     2281 tgatcgcttc ggcagcgacc ccgacgcaca gcgcagcgcc caacgctgcc ggaaccagca
     2341 gaacctcggg caccggcagc gccaccgacg gggccaacag gccggcgagt gcaacgagcc
     2401 cgaaacccac aagcgccgag acgagtccga acaacgtcca acgaaacacc ggaccgaccg
     2461 tgatcaggac ggcggcagcg atcgccacga gcaacccgcc gctcaggaag gtcccatggg
     2521 cacccgtgtc gaacaccagc gcctcagaga cgccgatcgg agcgccacgc ccggcggcat
     2581 tgcccaacaa gacctcaaca cggttcgccc cgcccaacac cgagatggtc cacggcaggt
     2641 gcaccaacgc cgacactgcg agcgaaccga gggtgacaac accgagccgg ccgagattgc
     2701 cgcgggaccc ggcgccgacc acgaccgcca tcacgacagc caacagcgcc accaccaaga
     2761 cgatcaccgg cgcgacggtg gcgccgatgg cgacgatcaa cccgagcgcg aacgtctgtt
     2821 gaagcaccga acgctcggca cgttcggtgt cggcgaacgg ttcgaacgct ccggccgcgg
     2881 cgaggcgcgc cacgatcgac ggggcaagcg cccagacgac gagcgcctgc catcgcccct
     2941 cggcgatggc gttgtacgga atcggattgg cgaggtaagc gaccaaaacg gccgtccgag
     3001 cgagcggcga cccgacgccg cgcagcaggc gccaggcgcc gagtgccccg acgccgagcg
     3061 gggcgaggaa caccacggtt cgaaccagcc cctccgaacc gaacgtcatc cagttcaagg
     3121 caccgaccac gaaagccacc gtgggggacc ctccggattg gccgagaccg gcgcttcgcc
     3181 agccgctcca ccactcccgc agcagatcgc cggagggaga attggtgacg aactcgcgaa
     3241 ccaccggaac cccgccggtg atcagcgacc gcgaccctcc cacgagcacg accgcggcga
     3301 tgaggaaggc accgatgccc caaattcccg tgttgtgtgc atcgttgggg cctgacatcc
     3361 ctcgcgctgc ggcggcgacc gcctggccga gcgaacgatc tctcgcggtg taggcgtgga
     3421 agaagttggt gaagcgcgcg cttcccttga tctgcagacg tcggatctcg tcatcggggg
     3481 tacgccggga tttctgggtg gaacggcgaa tcgaaagggt ccgcggtaga tgcccgaggt
     3541 tccacatgaa tgcggtgaac acgtcgccgg cctgacgcca acgaccgcgc aacagcgccc
     3601 cgaccaggtc gagcaccccg agcaccatgg cctggggaag gattcgcagc agcgtcaggg
     3661 cgctgtaaca ggccaacatg atgcgcgcct ggtgccgcaa ggccagacgc aggccacgct
     3721 catgggcctt gtcgaaggtc gattcagcgt gatgaaacac ctgtgcggcg ggcatgacca
     3781 ccgtgcgtgc tccggcgacc cgggcacgcc agcacaggtc gagttcctcg cccgagccgt
     3841 cgatgtcggt ggagaacccg ccgagatccc gaaagagatc gcagcgcacc aacatggccg
     3901 cacccgacac cacgaacgcc tcacgaatgg cgtcgtgttg ggactggtcg agctcgcccg
     3961 gttcggcgta gggccacagg aagccgaact tatcgatcga ggcgccaacg tcggtcagcg
     4021 tccgacggtc ggcgccgtcg agcagcttcg ctccgacgat tccggcgttg gagcggaacg
     4081 cctcctcgac catcgcctgc accgcacctt tgcccagttc cacgtcatcg tgcagcacca
     4141 ggtagaacga cgcaccatcg acggtgccga gcgcggcgtt cgcggccaca gcgaagccgc
     4201 cggcgtcgat ccgcttgacg aacgccgtcg gcaacacctc agccacccgt tcggtggggt
     4261 cggagtcgga accggcatcg accacgaggg tgacgaggtt ggcgtactcc tgagccgaga
     4321 tcgacgccag aagatcgtcg aagcgagcgc ccgggttgcg ggtgacgatg acggcgacca
     4381 ccgccggaac cccaactccg gcgacgggcg attcggcctc gtcggcagcg tcgtagccct
     4441 ccacgaacgg cgttgcgtcg gggttccact cctcgtcggc ggacgcgggc gactcgacgg
     4501 aggaactcga cgatggacgg tccgatcgcg tcgtgtcgga gcgacgacgc cgagaaccgg
     4561 aagttgggga agctggacgg cgtgcagaca tagatgaagg gtgtggtgcg actgagcgct
     4621 gtgaatagta gaccgcgacg acttcacggc ggctccgcga cgactccgcc ccaacttcag
     4681 cggcaggccg ccccagtcga ccggaggttt ctctatcggc tggtaggatt tcgaggtcaa
     4741 cgcaccctcg gagctgccat ggcaggagcc ctcgccccaa tcgacctcga acacatgcga
     4801 cagcaccgcg cactcggcga cccgacccgc catgccgtgt tcgtccacct tcgcgaagcc
     4861 gaacgccctg tcgatgtggc cgaactcacc gccaagttcg ggctcaacca caacgccatc
     4921 cgccagcacc tggcgcgcct ggccgacgca ggtctgatca ccgacgagct ccgggcctcc
     4981 accggcccgg gacgaccggc aaagctctat cgcgtcgttc ccggtgcggc ccagcgttgg
     5041 ggcgggacca gcccgcacga gacgctggcg gacatgttgc tcgagatggt ccgaaccgga
     5101 cgctcagcgc tcgagatcgg gcgcgacacc ggtcgtcggc tcgctgccga caacgccccg
     5161 gcgggttccg ccgtcgagat cctcgacggg gtggccaggc gacttggctt tgaaccgtgc
     5221 gtcgaactca ccgaacgcgg agccgacatc gtgttggggc gctgcccatt cgtcgacccg
     5281 gccaccgcag cacccgaggt cgtgtgtgcg ttgcaccggg gcatcgcgga gggcatcgcc
     5341 gaggcgaccg acaccgacga cgtcatcgag gacctcgtca tgcgcccacc cgccaccgcc
     5401 gggtgccgca tcagggtcgc ttcgtcgaac tgacgggggt cgcgacgcgt tcaacctcag
     5461 gacggagcgg gtccaacgtc ggctacgcca tcctcgtcgt cgccatcctc gtcctcctcg
     5521 tcgtcgaaca agtgcgccca acacg
//
LOCUS       DAOOMA010000100         5528 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_17668_length_5528_cov_4.830075, whole genome shotgun sequence.
ACCESSION   DAOOMA010000100 DAOOMA010000000
VERSION     DAOOMA010000100.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 5528)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5528)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5528
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17668_length_5528_cov_4.830075"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..614
                     /gene="recR"
                     /locus_tag="PLP95_04120"
     CDS             <1..614
                     /gene="recR"
                     /locus_tag="PLP95_04120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HPB45019.1"
                     /translation="YAQSIQELIDAFGRLPGVGPKSAQRIAFHLLKVDKAESQRLIAA
                     IAAAIDKVGFCDRCFNFAEAELCAICADPGRDAHLICVVEEPRDVVAIERTREFKGRY
                     HVLGGAIDHLQGVGPEQLKIRELLTRIGAEEVTEVILCTNPNIEGEATALYLQRMLQI
                     PDLTVSRIASGLPVGGDLEYADELTLGRALEGRRVIGEQAGAR"
     gene            625..1032
                     /locus_tag="PLP95_04125"
     CDS             625..1032
                     /locus_tag="PLP95_04125"
                     /inference="COORDINATES: protein motif:HMM:NF013228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HPB45020.1"
                     /translation="MSAERQLISSGSPWELTYGYSRAVRFGSRVFVAGTAPQYEDGVC
                     PQDPILQARRCFEIVEKALADAGASFKDVVRTRMYLVDVGDFDAVAAVHGEIFHDVRP
                     ANTTVVVAALLDPAWKVEVEVEAIINKDRSPHF"
     gene            complement(1103..2410)
                     /locus_tag="PLP95_04130"
     CDS             complement(1103..2410)
                     /locus_tag="PLP95_04130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="YncE family protein"
                     /protein_id="HPB45021.1"
                     /translation="MIRTRRGPNLATRLAALSAGVVVVCSGCVGAYEVTTEDKSDHAA
                     LQVATADATSGEAEAADAPAGPPEQSSTDPATDGDDPAVIDSTVEAAAPAVLHPVPSD
                     QRQMAKLSVITDGPLTPKSVEASANGLFFAQNMMYSHTITVYDRSFALVKSIPDSINP
                     ADWGYPDHAGTTTGSPVEMAFSHDGTKAYVSNYEMSGPGFSNPGNDKCSKGSWDNSYL
                     YRVDTASLEIDQVIEVGPVPKYVAVTPDDATVLTTNWCGYDLSVVDAATGKETRRIPI
                     GRFPRGIAVMPDNTTVFVAVMGTKDIAVVNLVDDSVQWITGVGQGPRHLVLDPAGEFL
                     YATLNGEGKVAKIDVATRTVVAKVASPSQPRSMTIAPDGRSLYVVNYDSDAVSKIRTS
                     DMTQIQRLPTGHHPIGITYDRTDNRVWVACYGGSLYVFDDTAS"
     gene            complement(2496..4790)
                     /locus_tag="PLP95_04135"
     CDS             complement(2496..4790)
                     /locus_tag="PLP95_04135"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfatase"
                     /protein_id="HPB45022.1"
                     /translation="MPARIGMTIAESTPSFSPRPTPPPGAPNVVVIVLDDVGFGQLGC
                     FGSDIDTPNIDALAGEGLRYNRFHVTALCSPTRAALLTGRNHHSVGMGFLSDVPIGFP
                     GYDGRIPSSAATLPRILKDNGYSTFAVGKWHLIPRWEQTAAGPFDQWPLGLGFERYYG
                     FLAGDANQWTPELVCDNTFVDPPSTPEDGYHLTEDLVERSRQLILDQKHARPAKPFFL
                     YFAPGAMHAPHQAPQPWIDRYAGHFDDGWDRWRERMFAKQVTEGVVPADTVLTERPPW
                     VQEWASLCDDERRLFARQMECFAGFLSHTDDQIGRLLSVLDDIGERDNTIVMLISDNG
                     TSAEGGPIGSHNEHRFTHDRLDDFDDSLSRIDELGGVRAYNHYAWGWAWAGNTPFRLW
                     KRFAWLGGTRTPLIVSWPGAIESTERGSVRSQFCHAIDLFPTLLDAIGLQAPSTVDGV
                     DQQPVHGASLVASLNDVDAADPRDTQYFEMLGSRAIFHDGWKATTDHVGRQVSIEQEL
                     VTGSQDFATDRWSLFDLRNDFSESTDLAADHPETLRTLTDRWWVEAGRHQVLPLDDSF
                     IGRAIALEPNPNPPVHLSRYLPGAGPINEDALPGMGGGIVVHANVQVGDRADGIIAAL
                     GDWNSGWALYLIEGAPTVTFNLFGNPVTLRAPISLDPGDHRVTVRYRRDRHGSGGGPI
                     GIEVDGAEVAAGSIDDDLPFRWQIGGAGLRIGHDAGLPVCDDYEIPFTFDGTIVDVVF
                     EQPMFAPRTSAPPSEEIGAALHRE"
     gene            complement(4899..>5528)
                     /locus_tag="PLP95_04140"
     CDS             complement(4899..>5528)
                     /locus_tag="PLP95_04140"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012224825.1"
                     /note="Catalyzes the synthesis of acetoacetyl coenzyme A
                     from two molecules of acetyl coenzyme A. It can also act
                     as a thiolase, catalyzing the reverse reaction and
                     generating two-carbon units from the four-carbon product
                     of fatty acid oxidation; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HPB45023.1"
                     /translation="RAAAAQKDGKFDAEIVKVEIPQRKGDPLVFAEDEGVRAETTLES
                     LASLRPAFDPAGNITAANASQISDGGAAVVVASKAAADKLGVTPLGEIIGNGQVAGPD
                     TSLLTQPSRAIFQALERAGLGVGDVDLFELNEAFAAVGVASMNDLGITADVTNVNGGA
                     IALGHPIGMSGTRIVLTLLNELARRGGGTGAAALCGGGGQGDALLVKTI"
BASE COUNT          911 a         1707 c         1934 g          976 t
ORIGIN      
        1 actacgccca gtcgattcag gagttgatcg acgcgtttgg tcgccttccc ggggttggcc
       61 cgaagtcggc acagcgcatt gcctttcatt tgctgaaggt cgacaaggct gaatcccagc
      121 gcctgatagc ggccatcgcg gcggcgatcg acaaggtcgg attctgcgac cgctgtttca
      181 acttcgcgga agccgaactg tgcgcgatct gcgccgatcc aggccgtgac gctcatctca
      241 tctgcgtggt cgaggaaccc cgagacgtcg tcgccatcga gcgcactcga gagttcaagg
      301 gccgctatca cgttctcgga ggggcgatcg accacctcca aggcgtcggc ccagagcaat
      361 tgaagatccg cgagctgctc accaggatcg gtgccgagga ggtcaccgag gtgatcctgt
      421 gcaccaaccc caacatcgag ggcgaggcca ccgcgctgta tctccagcgg atgttgcaga
      481 taccggatct gacggtgtcg aggatcgcga gcggtctgcc ggtgggcgga gacctcgagt
      541 acgccgacga attgacgctc ggtcgagcgt tggagggtcg acgggtcatc ggggagcagg
      601 ctggcgcgag gtaacgtcgc ccccatgtcc gcagaacgtc agctgatctc ctccggctca
      661 ccctgggaac tcacctacgg ctactcacgg gccgtgagat tcggcagccg cgtgttcgtc
      721 gccggcacgg cgccgcagta cgaggacgga gtatgcccgc aggacccgat actgcaggcg
      781 cgccgatgtt tcgagatcgt ggaaaaggcg ctcgcagatg ctggcgcgtc gttcaaagac
      841 gttgtgcgca cgcggatgta cctcgtcgat gtgggcgatt tcgatgcggt cgcggcggtg
      901 cacggcgaaa ttttccacga cgtccgtccg gcgaacacca ccgtggtcgt cgcggcgctg
      961 ctggacccgg cgtggaaggt tgaagtcgag gtcgaggcca tcatcaacaa ggaccgctcg
     1021 ccccatttct gaccatctct gagccgctga ggtcgtcggt cgagtggtcg cgccgctggc
     1081 accgccttgg gaggcgtggt ggtcaggagg ccgtgtcgtc gaagacgtag aggcttccgc
     1141 cgtaacaggc cacccatacc cggttgtcgg tacggtcgta ggtgatgccg atcgggtgat
     1201 gaccggtcgg caggcgttga atctgggtca tatccgaggt gcgaatcttg ctgaccgcgt
     1261 ccgagtcgta gttcaccacg tacaacgagc ggccgtcggg cgcgatggtc atcgagcggg
     1321 gttggctcgg ggaggcaacc ttggcaacga cggtgcgggt ggccacgtcg atcttggcga
     1381 ccttgccctc accgttcagc gtcgcgtaga gaaactcccc ggcggggtcc aacacgagat
     1441 gtcggggacc ctggccgact ccggtgatcc actgcaccga gtcgtcgacg aggttgacca
     1501 cggcgatgtc tttggttccc atcaccgcca cgaacacggt cgtgttgtcg ggcatcacgg
     1561 cgatgccgcg ggggaaacga ccgatcggga tccgacgggt ctctttgccc gtggcggcgt
     1621 cgaccacgct gaggtcgtac ccacaccagt tggtggtgag caccgttgcg tcatcagggg
     1681 tgaccgccac gtacttcgga accggcccaa cctcgatgac ctgatcgatc tccaagctcg
     1741 cggtgtcgac gcggtacagg tacgagttgt cccacgatcc cttgctgcac ttgtcgtttc
     1801 ccggattgga aaagccaggg ccggacatct cgtagttgga gacataggcc tttgtgccgt
     1861 cgtgggagaa cgccatctcg accggtgagc cggtcgttgt ccccgcgtgg tccggatacc
     1921 cccagtcggc cgggttgatc gagtcgggga tcgacttcac gagcgcgaac gaccggtcgt
     1981 acaccgtgat cgtgtggctg tacatcatgt tctgggcgaa gaacagcccg ttcgccgagg
     2041 cttcgaccga cttgggggtg agcggcccat cggtgatgac cgagagcttg gccatctggc
     2101 gctgatccga tggcaccggg tgcagcaccg ccggagccgc ggcctcgacc gtcgagtcga
     2161 tcacggctgg atcatcgccg tcggtcgccg ggtcggtgct cgactgttca ggtggcccag
     2221 ccggagcgtc cgccgcttcg gcctcgccgg acgtcgcgtc agcggttgcg acctgaaggg
     2281 cagcatggtc ggacttgtcc tcagtagtga cctcgtaggc gccgacgcac ccgctgcaga
     2341 ccacgacgac gccagcggac agtgccgcga gtcgggtcgc gaggttggga ccgcggcggg
     2401 ttcgaatcac ggcgacagtc ttgcatccgc cgtcgccggc cagcgacgtg gtgggctcac
     2461 accatggttc cgcaccgagg ttcggcgacg gtcggctact cacgatgcaa cgctgcccca
     2521 atctcctcgg agggtggagc cgaggttcgc ggcgcgaaca tcggctgttc aaagaccacg
     2581 tcgacgatgg tcccatcgaa ggtgaacgga atctcgtagt cgtcgcagac cggcaggcct
     2641 gcatcgtggc cgatccgcag cccggcaccg ccgatctgcc agcggaacgg taggtcgtcg
     2701 tcgatcgatc ccgcggcgac ctccgctccg tcgacctcga tgccgatcgg tccgccaccg
     2761 gagccgtgac ggtctcggcg gtacctcacg gtgacgcgat ggtcgccggg atcgaggctg
     2821 atcggcgccc gcagggtgac cgggttgccg aacaggttga acgtcaccgt cggtgcccct
     2881 tcgatcaggt agagcgccca cccgctgttc caatctccga gcgcggcgat gatgccgtcg
     2941 gcgcggtcgc cgacctgcac gttcgcgtgg accacgatgc ctcctcccat cccaggcagc
     3001 gcatcttcat tgattgggcc ggcgccgggg aggtaccgcg acaggtggac gggcggattc
     3061 ggattgggtt cgagcgcgat cgctcgtccg atgaagctgt cgtcgagcgg gagtacctga
     3121 tggcgccccg cctcgaccca ccatcgatcg gtgagtgtcc gcagggtctc cgggtggtcc
     3181 gcggcgaggt cggtcgattc ggagaagtcg ttgcgcaggt cgaacagcga ccatcggtcg
     3241 gtggcgaaat cctggctgcc ggtgaccagt tcctgttcga tgctgacctg gcggcccacg
     3301 tggtcggtgg tcgccttcca gccgtcgtgg aagatcgctc ggctcccgag catctcgaag
     3361 tactgcgtgt cgcggggatc cgcggcatcg acatcgttga gcgaggcgac gagtgaagcg
     3421 ccgtgaaccg gttgctggtc gaccccatcg acggtgctcg gagcctgcag gccgattgcg
     3481 tcgagcaggg tggggaacag gtcgatggca tgacagaact ggctgcgaac agaaccgcgc
     3541 tcggtcgact cgatggctcc gggccacgaa acgatcagcg gggttcgggt cccgccgagc
     3601 caggcgaagc gtttccacag tcgaaacggc gtgttgccgg cccaggccca accccacgcg
     3661 tagtggttgt aggcacgcac gccaccgagc tcgtcgatgc gcgagagcga gtcgtcgaag
     3721 tcgtcgaggc gatcgtgggt gaagcggtgc tcgttgtggc tcccgatcgg cccgccctcg
     3781 gcgctggtcc cgttgtcgct gatgagcatc acgatcgtgt tgtcgcgttc gccgatgtcg
     3841 tccagcaccg acaacaggcg tcctatctgg tcgtcggtat ggctgaggaa cccggcgaag
     3901 cactccatct gacgggcgaa caggcgacgc tcgtcatcgc agagcgatgc ccactcctgc
     3961 acccacggcg gacgctcggt caacaccgtg tcggcgggga ccactccctc ggtcacctgc
     4021 ttggcgaaca tgcgctcgcg ccaacgatcc caaccgtcgt cgaagtggcc ggcgtaccga
     4081 tcgatccacg gctgcggggc ctgatgcggt gcgtgcatcg ctcccggagc gaagtagaga
     4141 aagaacggtt tggccggtcg ggcgtgcttc tggtcgagga tgagctggcg cgagcgttcg
     4201 acgaggtcct cggtgaggtg gtagccgtct tcaggagtcg acggcgggtc gacgaaggtg
     4261 ttgtcgcaca ccagctcggg ggtccattgg ttcgcgtctc cggcgaggaa cccgtagtag
     4321 cgctcgaaac cgagaccgag gggccactgg tcgaaaggtc ccgccgcggt ctgttcccat
     4381 cgggggatga ggtgccactt gccgacggcg aaggtggagt acccgttgtc tttcaggatt
     4441 cgcggcaacg ttgccgccga cgaggggatt cgcccgtcgt agccgggaaa cccgatcgga
     4501 acgtcgctga ggaatcccat ccccaccgag tgatgattgc gcccggtgag cagcgcggca
     4561 cgggtcggag aacacagcgc cgtgacgtga aagcggttgt agcgcaagcc ttcgccggcg
     4621 agcgcgtcga tgttcggtgt gtcgatgtcg gaaccgaagc agccgagctg gccgaaaccg
     4681 acgtcgtcga gcacgatcac cacgacgttg ggggcgccgg gtggcggagt cggccgcggc
     4741 gagaagctcg gcgtcgattc ggcgatggtc attccgatgc gtgcgggcat ggggcctcca
     4801 tggagatggg cagcgcgtcg gggggtggtg agggtcgttc tgtagtcgtt tggggctgcc
     4861 atggcggccc caaacgacta cagaacactg acggtacgct agatggtctt gacgaggagg
     4921 gcgtcgccct ggccgccgcc gccgcacaac gcagcagcgc cggtgccgcc gccacgccgg
     4981 gcgagttcgt tgaggagggt caacacgatt cgcgtgccgg acatgccgat cgggtgaccg
     5041 agggcgatgg cgccgccgtt gacgttggtg acgtccgcgg tgatgccgag gtcgttcatc
     5101 gaggcgacgc cgaccgcggc gaaggcttcg ttgagttcga acaggtcgac gtcgccgacc
     5161 ccgagcccgg cgcgctcgag cgcctggaag atggcgcggc tcggctgggt gagcagcgag
     5221 gtgtccgggc cggcaacctg gccgttgccg atgatttcgc caaggggcgt cacgccgagc
     5281 ttgtccgcag cggccttcga cgcgaccacg acagcggctc cgccgtcgga gatctgcgac
     5341 gcgttggcgg cggtgatgtt gccggcaggg tcgaacgccg ggcgaagtga ggcgagggat
     5401 tcgagcgtgg tctcggcacg aacaccctcg tcctcggcga acacgagcgg gtcgcccttg
     5461 cgctggggaa tttcgacctt cacgatttcg gcgtcgaact ttccgtcctt ctgagcggcc
     5521 gcagcgcg
//
LOCUS       DAOOMA010000101         5376 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_18318_length_5376_cov_4.600639, whole genome shotgun sequence.
ACCESSION   DAOOMA010000101 DAOOMA010000000
VERSION     DAOOMA010000101.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 5376)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5376)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5376
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18318_length_5376_cov_4.600639"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..971
                     /locus_tag="PLP95_04145"
     CDS             <1..971
                     /locus_tag="PLP95_04145"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HPB45024.1"
                     /translation="LTASPFRPWRAMMHAAAQWTSLSWLFCGGKVAMVPGSFDPLKTW
                     QMIETHKVGLVIIVGDAMAKPLIDAWDEHGPFDISSMYAVGSGGAPLNPVYKKRLFEI
                     APNAMITDGFGSSETGAQGSHRIFPGDEADGKTRFSPMDAGTKVLSDDGREVEAGSGV
                     VGKVALTGHIPLRYYNDEAKTAETFVEVDGTRWVVTGDMATVEADGVITLLGRGNQVI
                     NTGGEKVYPEEVESVLRTHPDVFDAVVTGVADERFGQCVCAVVAVVDGAEVTLEGLRE
                     YARSELAGYKLPRELVLVDQVVRSPVGKADYVWASTTASAALGSRS"
     gene            968..2134
                     /locus_tag="PLP95_04150"
     CDS             968..2134
                     /locus_tag="PLP95_04150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA-transferase family protein"
                     /protein_id="HPB45025.1"
                     /translation="MNERPGALAGIKVIELGSLGPGPFCAMMLADHGADVLRVERPGG
                     TTASPIAKSDRFAAWDVMSRGRRSVGIDLKHPQGRDLIKRLAADADVLIEGFRPGVAE
                     RLGLGPDDLRDANERLIYGRMTGWGRGGPLSPRAGHDINYLSIAGVAAHIGRNGEVPT
                     PPLNLVADFGGGAMMLAFGICAALVSRSVSGLGQVVDAAMIDGAALLMAPLVGAHAMG
                     FWSEERGTNLLDSGAPFYDFYETSDGGYVSVGAIEPQFFAALVTTLGLDDSWCGRQND
                     EAAWPALRAELVERFGSRTRDDWAAIFAEVDACVAPVLTMAEAPHHPHNAAMQNWIEL
                     GGVRQPAPAPRLERTPAPMPTLPEAPAASTDSKLVDWGVTASEVARLREVGAIG"
     gene            complement(2170..3081)
                     /locus_tag="PLP95_04155"
     CDS             complement(2170..3081)
                     /locus_tag="PLP95_04155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB45026.1"
                     /translation="MTCRQPSAPQKSSRPERRVRFAIATLILAWATGAGFVPAGASTA
                     AGEVPVDQTEILSNVGTVAPEATPSAKSIGSVVLSGDKMAVVIPLADERAFPLGSEAT
                     FQRRGSTEASHDCYVAARSPRGGVAALRCRGLDGAGFRAVRPAVAAGAMLHVVTDSIL
                     DATASGYDTTSGRLNVVVQAGCCLPGTPVLNPDMDLVGLVAAATQPDQTPTVSTSAVH
                     GNAVASIAQDAVAHRDKLIWQALAVSKWWVVLSLLAGALLGFGWALLARRPYLWRMIG
                     VALLGGAVAVTVVAALSQGDNPGRFLG"
     gene            complement(3035..3787)
                     /locus_tag="PLP95_04160"
     CDS             complement(3035..3787)
                     /locus_tag="PLP95_04160"
                     /inference="COORDINATES: protein motif:HMM:NF018066.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetoacetate decarboxylase family protein"
                     /protein_id="HPB45027.1"
                     /translation="MAGLTPQPDGSYLVMGQRVAMPVLVRKAKQAAVMFLVDHNAAQR
                     MVAPTGLKVVRQLGGKAIVTLALVDYIDNDLGTYTELGLVFMVHDSGATPTSANYGNR
                     KAVTTYIHRLPVNGEFTKEAGYGIWGFPKWVADLSVDFDRNGVTAQLKSGDEVVMTVR
                     AKRGPIRVPRRAMPMNAYTCDEDGIVRRTPWTTDGTQKQQVRLGGATVEVGYGHPLAD
                     ELRALGFPKRCLATIFDDDMQATFGAPEIVSA"
     gene            complement(3787..>5376)
                     /locus_tag="PLP95_04165"
     CDS             complement(3787..>5376)
                     /locus_tag="PLP95_04165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012230046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HPB45028.1"
                     /translation="RTALVDRDRRLTFAELDERANRVAHLLTELGIGPGDHVGIYAQN
                     SLEWFETMMGAFKISAVPVNINYRYVADELTYLFDNADLKAVVFDAQYAPLIAEVSER
                     IPALTTFVHYEVFPEEGAAATHAPVLAALGSVSLDEGTAAASPSRDGLPTRSGDDIFM
                     IYTGGTTGMPKGVMWRHEDVYFALGQGINSVTGERVDSDHEMADKAKANDFFLSIFFI
                     APFMHGATQLGSLGQMLQGNTIVVTPKFDPHDVWATVERDGVNTIMITGDAMGRPLVE
                     HLEANPGTYDTSALIALSSSAAIFSPTIKDRFLELFPNLVMTDAIGSTESGSTGIRFV
                     GKGAAQNPNGGPTVTPREDTVILDDEGGLLAPESLGTGILGRGGNIPLGYYKDDKKTA
                     ETFITAADGKRYAVSGDYARWEPSGEITLLGRGSTVINSGGEKIHPEEVEQALKDHPA
                     VYDCIVVGVPDERWGQRVAAVVQFREGQTAELDQLADHCRSFVAGYKVPRQLTVVDHI
                     ERSPSGKPDYPWAVNLAKGDR"
BASE COUNT          877 a         1633 c         1883 g          983 t
ORIGIN      
        1 cgctgacggc aagcccgttc aggccgtgga gagcgatgat gcatgcagcc gcgcagtgga
       61 cctcgttgtc gtggctgttt tgcggcggca aggtcgcgat ggttccggga agcttcgatc
      121 cgttgaagac ctggcagatg atcgagaccc ataaggtcgg gctggtcatc atcgtcggcg
      181 acgccatggc gaagccactg atcgatgctt gggatgaaca cggccccttc gacatctcct
      241 cgatgtacgc ggtcgggtcc ggtggtgcgc cgctgaaccc cgtctacaag aagcgactct
      301 tcgagattgc tccgaatgcg atgatcaccg acggcttcgg gtcctcggag acgggcgccc
      361 aggggtcgca tcgaatcttt cccggcgacg aagccgacgg gaagacccgg ttttctccca
      421 tggacgcagg taccaaggtc ctctccgacg acggccgcga ggtcgaggcc ggttcggggg
      481 tcgtcggcaa ggtggcgctc accggccaca tcccgcttcg gtattacaac gatgaggcca
      541 agaccgccga aacctttgtc gaagtcgacg gcacgcgatg ggtggtgacc ggcgacatgg
      601 ccaccgtcga ggccgacggg gtcatcacct tgttgggccg tggcaaccag gtgatcaaca
      661 ccgggggcga gaaggtgtat cccgaggagg tcgagtcggt gctgcgcacg catcccgacg
      721 tgttcgacgc cgtcgtcacc ggagtcgcag atgaacgatt cggacagtgt gtttgcgcgg
      781 tggtcgccgt cgtcgatggt gcggaggtca ccctcgaggg gctccgcgaa tacgcccgtt
      841 cggagcttgc cggttacaag ttgccgcgcg aactcgttct ggtcgatcag gtggtccggt
      901 caccggtcgg caaggccgac tatgtgtggg cgtcgaccac agcgagtgcc gcgctcggct
      961 cacgttcgtg aacgagcgac ccggtgcgct tgccgggatc aaggtgatcg aactcggttc
     1021 gctcgggccg gggccgtttt gcgcaatgat gctcgcggat cacggcgcgg atgtccttcg
     1081 ggtcgaacgg ccgggcggca ccactgcgtc gccgatcgcc aagagcgacc ggtttgccgc
     1141 gtgggacgtc atgagccgcg gtcgtcgcag cgtgggaatc gatttgaagc acccgcaggg
     1201 ccgcgacctg atcaagcggc tcgccgctga cgcggatgtg ttgatcgaag ggttccgacc
     1261 cggggttgcc gaacgcctcg ggctcggccc cgatgacctc cgcgacgcga atgaacggct
     1321 gatctacgga cggatgacgg gctgggggcg cggcggtccg ttgtcgccgc gtgcaggcca
     1381 cgacatcaac tacctgtcga ttgctggcgt tgcggcgcac atcgggcgca atggcgaggt
     1441 cccgaccccg ccattgaacc tcgtcgccga tttcggcggc ggtgcgatga tgctggcgtt
     1501 tggaatctgc gcggcattgg tgtcgcgctc ggtgagcggg ctcggccagg tggtcgacgc
     1561 ggcgatgatc gatggggcag cgttgttgat ggccccactc gtcggcgcac acgcgatggg
     1621 gttctggtcc gaagaacgtg gcacgaacct gctcgattcg ggagcgccgt tctacgattt
     1681 ctacgagacc tccgacggcg gttacgtatc ggtcggggcc atcgagccgc agttctttgc
     1741 tgctctggtg acgacgctag ggctcgacga ctcgtggtgc ggccgacaaa acgacgaggc
     1801 ggcgtggccc gcgttgcgtg ccgagttggt cgagcgcttc gggtcgcgga ctcgcgacga
     1861 ctgggccgcg atcttcgccg aggtcgatgc ctgcgttgct cccgtgttga cgatggcgga
     1921 ggccccacat cacccccaca acgctgcgat gcagaactgg atcgaactcg gcggagtgcg
     1981 acagccagcc ccggcgccgc ggctcgaacg cacccctgct cccatgccga ctctgccgga
     2041 ggcccctgcc gcgagtaccg actccaagct tgtcgactgg ggtgtgacgg cgagcgaagt
     2101 ggcgcggttg cgtgaggttg gggcgatcgg ctgacccgtc gcagcggagc gggttgctct
     2161 gcgtgacaat cagccgagga agcggcccgg gttgtcgccc tgggacagcg ctgcgacgac
     2221 ggtgacggcg accgcgccac ctagaagtgc gaccccgatc atgcgccaca gatagggtcg
     2281 tcgtgccagc agcgcccacc cgaaccccaa cagtgcgccg gcgagcagcg acagcaccac
     2341 ccaccatttg gagacagcaa gcgcctgcca gatgagcttg tcccgatggg cgacagcgtc
     2401 ttgtgcgatc gaagcgaccg cattgccgtg gaccgcagag gttgaaacgg tgggggtctg
     2461 gtccggttgg gttgccgcgg cgaccagccc gacgaggtcc atgtcgggat tcagcaccgg
     2521 ggtcccgggc aggcaacagc ccgcctgtac gaccacgttg aggcggccgc tcgtcgtgtc
     2581 gtagccggag gcagtggcgt cgagaatgga gtcggtgacg acgtggagca tcgcgccggc
     2641 ggcgacggcc gggcgaacgg cacgaaagcc agcgccgtcg agtccccggc aacgcagtgc
     2701 ggccacgccc ccgcgcggtg accgtgcggc gacgtagcag tcgtgagacg cctcggtgga
     2761 acccctccgt tggaaggttg cctcgctccc taacggaaac gctcgttcgt cggcgagcgg
     2821 tatgaccacg gccatcttgt cgccggagag cacgacggat ccgatggact tggccgacgg
     2881 ggttgcttcg ggggcgacgg taccgacgtt ggagagaatt tcggtctgat cgacgggcac
     2941 ttcgcctgcg gcggtcgagg cgccagcggg tacgaatccc gctccggttg cccaggcgag
     3001 gatcagcgtc gcgatcgcga agcgaacacg acgctcaggc cgagacgatt tctggggcgc
     3061 cgaaggttgc ctgcatgtca tcgtcgaaga tggtagccag gcagcgcttc ggaaacccca
     3121 gtgcgcgcaa ctcgtcggcg agcgggtggc cgtagccgac ttcgacggtg gccccgccga
     3181 gtcgaacctg ctgcttctgg gttccgtcgg tggtccaagg tgtccggcgc acgatgccgt
     3241 cctcgtcgca ggtgtaggcg ttcatcggca tggcgcggcg cggaactcgg atgggtccgc
     3301 gtttcgcgcg cacggtcatt accacctcgt cgccggattt gagctgcgcg gtgaccccgt
     3361 tgcggtcgaa atcgacgctc agatcggcga cccacttcgg gaatccccag atgccgtagc
     3421 cggcctcctt ggtgaattcc ccgttcacgg gaagccgatg gatgtaggtc gtgacggcct
     3481 tccggttgcc gtagttggcc gaagtcgggg tggccccgct gtcgtgcacc atgaacacca
     3541 ggccgagttc ggtgtaggtg ccgaggtcgt tgtcgatgta atcgacgagg gccaaggtca
     3601 cgatcgcctt gcctccgagt tgtcggacga ccttgaggcc ggttggtgcc accatgcgtt
     3661 gggcggcgtt gtggtcgacg agaaacatca ccgcggcctg tttggccttg cgcaccagaa
     3721 ccggcatggc gacgcgttgt cccatgacga ggtagctacc gtccggctga ggggtgagtc
     3781 cggccatcag cgatctccct tggcgaggtt gacggcccag gggtagtccg gcttgcccga
     3841 gggtgagcgc tcgatgtggt cgacgacggt gagttggcgc ggcactttgt atccggcgac
     3901 gaaggaccgg cagtgatctg cgagctggtc gagttcggct gtttggcctt cgcggaactg
     3961 cacgacggcg gcgacgcgtt gtccccaccg ctcatctggc acgccgacga cgatgcagtc
     4021 gtacacggcc gggtggtcct tgagcgcctg ttccacctct tcggggtgga tcttttctcc
     4081 gcccgagttg atgacggtcg agccccgacc gagcaacgtg atttccccgg agggttccca
     4141 tcgtgcgtag tctccgctaa ccgcgtagcg cttgccatcg gcggcggtga tgaaggtttc
     4201 tgccgtcttc ttgtcgtcct tgtagtaacc cagcggtatg ttgccgcctc gcccgagaat
     4261 gccggtaccc aacgattccg gggccaggag gccgccttca tcgtcgagga tcacggtgtc
     4321 ctcgcgagga gtcacggtcg gtccgccgtt ggggttctgc gcagcgccct tgccgacgaa
     4381 gcggatgccg gtggagccgg actcggtgga tccgatggca tcggtcatca ccagattcgg
     4441 aaacaactcg aggaagcggt ccttgatcgt ggggctgaag atcgcggccg aggaggacaa
     4501 cgcgatgagc gccgaggtgt cataggttcc ggggttggcc tcgaggtgtt cgaccaacgg
     4561 tcggcccatg gcatctccgg tgatcatgat cgtgttgacc ccatcgcgct cgacggtcgc
     4621 ccagacgtcg tgcgggtcga acttgggggt gacgacgatc gtgttgccct gaagcatctg
     4681 cccgagagat ccgagttggg tcgcgccgtg catgaacgga gcgatgaaga agatcgacag
     4741 aaagaaatcg ttcgctttgg ccttgtcggc catctcgtga tcgctgtcca cccgctcgcc
     4801 ggtgaccgag ttgatgccct gcccgagagc gaaatacacg tcctcatggc gccacatgac
     4861 ccctttgggc atcccggtcg taccgccggt gtagatcatg aagatgtcgt cgccgctgcg
     4921 tgtgggcaag ccatcgcgcg acggggaagc cgcggcggtt ccctcgtcga ggctcaccga
     4981 accgagtgcg gcgaggaccg gcgcgtgagt agctgccgcg ccctcctcgg ggaacacctc
     5041 gtagtgaacg aacgtcgtca gagcgggaat tcgctcgctc acctcggcga tgagtggagc
     5101 gtactgggcg tcgaatacca ccgccttgag gtcggcgttg tcgaacaggt acgtgagctc
     5161 gtcggcgacg tagcggtagt tgatgttgac cggaacggca ctgatcttga aagcgcccat
     5221 catggtttcg aaccactcga ggctgttctg ggcgtagatg ccgacgtggt cgcctggccc
     5281 gatgccgagt tcggtcagaa ggtgtgcgac gcgattcgcc cgctcatcga gttcggcgaa
     5341 ggtcagccgc cgatcgcgat cgactagcgc ggtccg
//
LOCUS       DAOOMA010000102         5375 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_18328_length_5375_cov_3.590789, whole genome shotgun sequence.
ACCESSION   DAOOMA010000102 DAOOMA010000000
VERSION     DAOOMA010000102.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 5375)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5375)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5375
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18328_length_5375_cov_3.590789"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2812)
                     /gene="hrpA"
                     /locus_tag="PLP95_04170"
     CDS             complement(<1..2812)
                     /gene="hrpA"
                     /locus_tag="PLP95_04170"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR01967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase HrpA"
                     /protein_id="HPB45029.1"
                     /translation="MSSLPQPGASPDPQPTPAAPSVGERSASVVAPDLPIHERHDEIV
                     AAIRQHQVVVIAGETGSGKSTQLPKLCLEAGRGAVDPDTGRARWIGHTQPRRIAARSI
                     AERVAEELDETIGGIVGYKVRFNDEVGASTRIKVMTDGILLAEVQRDRNLRAYDTVII
                     DEAHERSLNIDFLLGYLKRLLPKRPDLHVIITSATIDTARFSAHFDDAPVIVVEGRTY
                     PVEVRYRPLDPAEALAAAGVANAGDDLGDPLDIAEGICAAVDELSSEIDGDVLVFCSG
                     EREIREAAAALEDLKLPDTEVFPLFARLSAAEQHRVFEPHRGRRIVLATNVAETSLTV
                     PGIRAVIDPGTARISRYSNRTKVQRLPIEEVSQASANQRAGRCGRVGPGICIRLYSEE
                     NFVARPEFTEPEIRRTNLAAVILQMANLGLGDASRFPFVEAPSERNIRDGIALLEELG
                     AVDPRRHGTRRWLTPIGRQLVRMPVDPRFGRMVLEAVDNGALDEVMIIAAAMSIQDPR
                     ERPFDNRTAADQSHARFKHPDSDFLTYLNLWNHLVAEREARSGNQYRRMCRQEFLNYN
                     RIREWQDIYGQLRRSTKALGFIPNTAPAAADQVHISLLAGLLSHIGMRNNAQRSGVET
                     RQHRSHGHREGGDRRASRDYIGARNARFTIAQGSSLTKSPPEWVMAGELVETDRMWAR
                     TLTKIDPAWAERLGEHLCKYSYGEPWWDRGRGAALVTERVTLYGLPIVGDRRVQLASV
                     DPALARDLFIQHALVEGDWDAHHKFIDRNDRVASEAAQVEARARRGDLVVTHHEIFDF
                     YDERVPPTVTNTAAFNEWWKAERRTNGKLLDLTLDDLLVDDPDEIIDHDAFPDTWNVD
                     GHDLAVTYEFDPLSDHDGATITIDVGVLGQIQPDVFDWNVPGFRDEMVDALIRTLPKQ
                     LRKLFVPVPETVELIRDDLEANG"
     gene            2933..4240
                     /locus_tag="PLP95_04175"
     CDS             2933..4240
                     /locus_tag="PLP95_04175"
                     /inference="COORDINATES: protein motif:HMM:NF014680.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HPB45030.1"
                     /translation="MEWLVVILLVALIVLTTLSLVRREGGGVSGADMAAVQVEAARRE
                     GELREALARAEASRASDQQQLAAFGAVSQQALAGQSQQFLQLAETRYGALANGTSTLL
                     NQHSERIAAALDALGARLSSLERERSQATNSLESMVRELTQATQATRSEAARLHSALR
                     DSRVRGTWGEVQLRRVLELAGLERHTDFVEQQSSVADGQTIRPDVVVGLPNGRSIVID
                     AKVPLDRYLEAANSDDPDAERQLLVEHARSLAGHVNALSNRNYTSGVDGAIDLVVLFL
                     PGDAFLTAALDADPSLFETSATKGVVLTTPSSLLPLLRGIALGWRERRAEEEAAEIQR
                     LGAELHERIANFLDGYAAVGARLNSAVESYNRSVGSLEGRMLVTARKLAEVGAGSTKK
                     VPEMATVAALARTPTVPVLASGGSGGGDPDAEPGGELSLPSGE"
     gene            complement(4218..>5375)
                     /locus_tag="PLP95_04180"
     CDS             complement(4218..>5375)
                     /locus_tag="PLP95_04180"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HPB45031.1"
                     /translation="ASGRNGGWCSALFATSSSTLAHRFGAQAAAAMQAAMRATVDEVA
                     RAAAEDDIDCDFAKGGTLTVATLPQHVDAIRSAALAETDAHWLDPTAATARFATASNH
                     GAAYTPHCAAVHPGKLVRGLAATVGERGVRIAERSPVAKVLPGRLLLRDGTVVRANNI
                     IRATEGYTSTITGFRRRVVPIYSLMIATEPLADSVWDEIGLRSRETFTDGRRVIIYGQ
                     RTADGRIAFGGRGAPYHFGSRIRPGFDIDRRVFDQIETTLRTLLPQIGGARITHRWGG
                     PLGVPRDWMSSVGFDAVSRIGWAGGYVGDGVSTTNLAGRTLADLVLGRPTELTSLPWV
                     QHRSPNWEPEPARWIGINAGRALAEAADRIENRGHHVPALLERTLTRLTGG"
BASE COUNT          832 a         1633 c         1958 g          952 t
ORIGIN      
        1 tgccgttcgc ctcgaggtcg tcgcgaatga gttcgaccgt ctcggggacg gggacgaaca
       61 gcttgcgaag ttgcttcggc agcgttcgga tgagtgcgtc gaccatctcg tcgcggaatc
      121 ccgggacgtt ccagtcgaac acgtcgggct ggatctggcc gaggaccccg acgtcgatcg
      181 tgatggtcgc accatcgtga tcgctgaggg ggtcgaattc gtaggtgaca gcgaggtcgt
      241 ggccgtcgac gttccacgta tcgggaaagg cgtcgtggtc gatgatctcg tcggggtcgt
      301 cgacgagcag gtcgtcgagt gtgaggtcca agagcttgcc gttggtgcgc cgttcggcct
      361 tccaccattc gttgaacgct gcggtgttgg tgaccgtcgg aggcacgcgc tcgtcgtaga
      421 aatcgaagat ctcatggtgg gtgaccacga ggtcgccacg gcgtgcgcga gcctcgacct
      481 gtgcggcctc ggacgcgacc cgatcgttgc ggtcgatgaa cttgtggtga gcgtcccagt
      541 cgccttcgac gagagcgtgt tggatgaaca ggtcgcgggc gagcgctggg tcgacggacg
      601 cgagttgcac acgacggtcg ccgacgatcg ggaggccgta cagggtgacg cgttcggtga
      661 cgagtgccgc tcctcgcccg cggtcccacc acggctcgcc gtaggagtac ttgcacagat
      721 gctcgccgag ccgttcggcc cacgctggat cgatcttggt gagcgttcgc gcccacatcc
      781 ggtcggtctc gacgagttca cccgccatca cccattcggg cggagacttc gtcaacgagg
      841 atccctgggc gatggtgaag cgagcgtttc gtgcgccgat gtagtcgcgt gatgcccggc
      901 ggtcgccgcc ttctcgatgg ccgtggcttc ggtgctggcg ggtttcgacg ccgctgcgtt
      961 gggcgttgtt gcgcatgccg atgtgggaca gcagcccggc gagcagcgag atgtggacct
     1021 gatcggctgc cgccggggcg gtattgggga tgaagccgag agctttggtg gatcgtcgga
     1081 gctgtccgta gatgtcctgc cactcgcgga ttcggttgta gttgaggaac tcctggcgac
     1141 acatcctgcg gtactgattg cccgaccgcg cctcgcgctc tgcgacgagg tggttccaca
     1201 ggttgagata cgtcaggaag tcgctgtcgg ggtgcttgaa ccgagcgtgc gactggtcgg
     1261 ccgcggtgcg attgtcgaac gggcgctcgc gtgggtcctg gatcgacatg gcggcggcga
     1321 tgatcatcac ctcgtcgagc gcgccgttgt cgaccgcttc gagcaccatg cgtccgaacc
     1381 gtgggtccac cggcatgcgc acgagttggc ggccgatcgg ggtgagccac cggcgggtac
     1441 cgtgtcggcg cggatcgacg gcgccgagtt cctcgagcag tgcgatgccg tcgcggatgt
     1501 tgcgttccga tggggcctcg acgaacggaa acctcgacgc gtcgccgagc ccgagattcg
     1561 ccatctgaag gattaccgcc gcgaggttgg tgcggcggat ttcgggttcg gtgaactcgg
     1621 gccgggcgac gaagttctcc tcgctgtaga gccggatgca gatacccggg cccacccgac
     1681 cacagcgtcc ggcccgttgg ttcgccgagg cttggctgac ctcttcgatg gggaggcgct
     1741 ggactttcgt gcgattcgag taacgggaga tgcgggcggt tccggggtcg atgacggccc
     1801 ggatgccggg gacggtcagc gaggtctcgg ccacgttggt ggccaacacg atgcgccgcc
     1861 ctcggtgagg ttcgaaaacg cggtgctgtt cggccgcaga gaggcgtgcg aacagcggga
     1921 agacctcggt gtcgggcagt ttcagatcct caagcgctgc cgcggcctcg cggatttcgc
     1981 gttcgcccga gcagaagacg agcacgtcgc cgtcgatttc ggaggacaat tcgtcgaccg
     2041 cggcgcagat gccctcggcg atgtcgagtg gatcgccgag gtcgtctccg gcgttggcga
     2101 caccggcggc cgcgagggcc tcggcgggat ccagcggtcg gtagcggacc tccaccggat
     2161 aggtgcgacc ttcgacgacg atgacgggcg catcgtcgaa atgggcactg aagcgtgcgg
     2221 tgtcgatcgt cgcggaggtg atgatgacgt ggagatcggg tcgtttcggt aggaggcgct
     2281 tgaggtatcc aaggaggaaa tcgatgttga gcgatcgctc gtgggcctcg tcgatgatga
     2341 ccgtgtcgta ggcgcgcagg ttccggtcgc gttgaacttc ggccaacaag atgccgtcgg
     2401 tcatcacctt gatgcgcgtg gatgcgccga cctcgtcgtt gaaccggacc ttgtagccca
     2461 cgatgccgcc gatggtctcg tcgagttctt cggcgacgcg ttcggcgatc gaccgggcgg
     2521 cgatgcggcg gggctgggtg tggccgatcc agcgggctcg gccggtgtcg ggatcgacgg
     2581 caccgcggcc ggcctcgaga cagagcttcg gaagctgggt ggacttgccc gatccggtct
     2641 ccccggcgat gaccaccacc tggtgttgtc gaatggctgc cacgatctcg tcgtgtcgtt
     2701 cgtggatcgg taggtcgggt gcgacgacgc tcgcggagcg ttcgccgacg gacggtgcgg
     2761 ccggggtggg ttgagggtcc ggtgatgcgc ccggttgggg gaggctggac atggcgtcac
     2821 caggctacgt gggcgcggtc gagtccctgg cgccagttac tgagcttgct ggcggcgggg
     2881 cttggggccg ccgcatcttg agtgtcaggg gttgcccgta ccgtgcgaag ccatggaatg
     2941 gctcgtggtg atcctgttgg tcgcactcat cgtgttaacg acgctctcgt tggtgcgtcg
     3001 cgagggaggt ggcgtatcgg gtgccgacat ggccgcggtg caggtcgaag ccgccagacg
     3061 cgaaggcgaa ctgcgcgagg cactcgctcg ggccgaggct tcacgggcta gcgatcaaca
     3121 acaactcgcg gcattcggtg ccgtgtcgca gcaggcgctt gccgggcagt cgcagcagtt
     3181 tcttcaactg gcggagacgc gctatggggc gttggcgaat ggaacttcga cgctgctgaa
     3241 ccagcacagc gagcgcatcg ccgcggcgct cgacgcgttg ggggcgaggc tgtcctcgct
     3301 cgaacgcgag cggtcccagg cgacgaactc actggagtcg atggttcgcg agttgaccca
     3361 ggcgacccag gcaacccgaa gcgaggccgc gcgccttcac agcgcgctcc gagacagccg
     3421 ggtacgcggt acctggggtg aggtgcagct tcggcgagtc ctggaactgg cggggctgga
     3481 gcgacacacc gacttcgtcg agcagcagtc ctcggttgcc gatggccaga cgattcgccc
     3541 cgatgtcgtc gtcggactgc ccaatggccg atcgatcgtg atcgacgcca aggtgccgct
     3601 cgaccgctac ctcgaggccg ccaacagcga cgaccccgac gcagagcggc aattgctcgt
     3661 cgagcacgct cgctcgctcg ctggccatgt caacgcgctg tcgaaccgca actacaccag
     3721 cggggtcgac ggggcgatcg atctcgtcgt gttgtttctc cccggcgacg cgttcctcac
     3781 cgccgcgctc gatgccgacc cgtcgttgtt cgagacgtcc gcgaccaagg gggtggtgct
     3841 gaccaccccg agctcgctgc ttccactgct tcgcggaatc gcgctcggtt ggcgcgagcg
     3901 tcgcgccgag gaggaggccg ccgagattca acggctcggt gccgaactcc acgaacggat
     3961 cgcgaatttc ctcgatggat acgcggcggt cggagctcgg ctgaacagcg ctgttgagag
     4021 ctacaaccgg tcggtcggct cgctcgaagg caggatgctg gtaacggccc gcaagctggc
     4081 cgaggttggg gccggatcga ccaagaaggt gccggagatg gcaacggtgg cggcgctcgc
     4141 tcgcactccg acggtcccgg tcctggcgtc gggcggaagc ggcggcggcg acccggatgc
     4201 tgagccgggt ggagaactca gcctcccgtc aggcgagtga gcgtgcgttc gagcagggcc
     4261 ggaacgtgat ggccgcggtt ctcgatccgg tcggctgctt cggcgagcgc gcggccggca
     4321 ttgattccga tccaccgagc cggttcgggt tcccagttgg gggagcggtg ttgcacccag
     4381 ggcaacgagg tcaactcggt cggacgccca aggaccagat cggcaagggt ccgccctgcg
     4441 aggttcgtcg tcgacactcc gtcgcccaca tatccgcccg cccagccgat ccgcgagacc
     4501 gcgtcgaacc cgaccgagga catccagtcg cgtgggaccc cgagcgggcc tccccaccgg
     4561 tgggtgattc gagcaccccc tatctggggc aacagggttc gtaacgtcgt ctcgatctga
     4621 tcaaagaccc gtcggtcgat gtcgaagccg ggtcggatcc gagagccgaa atggtacgga
     4681 gctccgcgtc cgccgaacgc gatgcggcca tcggcggtgc gttggccgta gatgatgacg
     4741 cgacgcccgt cggtgaatgt ctcgcgggac ctcagcccga tctcgtccca cacggagtcg
     4801 gcgagcggtt cggtcgcaat catgagggag tagatcggga ccacccgccg tcgaaacccg
     4861 gtgatggtgg aggtgtagcc ctcggttgcg cggatgatgt tgttggcgcg caccaccgtg
     4921 ccgtcccgca gaaggaggcg gcccggaagc actttggcca ccggtgaacg ttccgcgatg
     4981 cggacgccgc gttcgccgac ggtcgcggcg agaccgcgaa cgagcttgcc cggatgaacc
     5041 gccgcacagt gtggcgtgta ggcggcccca tggttggagg cggtagcgaa tcgggcggtt
     5101 gccgcggtcg gatcgagcca gtgcgcgtcg gtctccgcga gcgcggccga ccgaattgca
     5161 tcgacgtgtt gcggcagggt cgcgacggtg agggttccgc ccttggcgaa gtcgcagtcg
     5221 atgtcgtcct cggccgcggc gcgggcgacc tcgtcgacgg tcgcgcgcat cgctgcctgc
     5281 atcgcagcag ccgcctgcgc cccaaaccgg tgggccagtg tcgacgacga ggtcgcgaac
     5341 aacgccgaac accacccacc gttgcgaccc gatgc
//
LOCUS       DAOOMA010000103         5367 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_18363_length_5367_cov_3.798758, whole genome shotgun sequence.
ACCESSION   DAOOMA010000103 DAOOMA010000000
VERSION     DAOOMA010000103.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 5367)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5367)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5367
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18363_length_5367_cov_3.798758"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1483
                     /locus_tag="PLP95_04185"
     CDS             <1..1483
                     /locus_tag="PLP95_04185"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HPB45032.1"
                     /translation="DDMTLRLRLTLAIVALTSVGLIVFSVVLTSFYAASQYDRLDERL
                     STTGPEAINLLAAEIGLEAPTPGRLGTGRHRVLGPGLDVSVELRDRNGEVLGSAVLLG
                     TENPPEFPATVNPERGTNLFFNVRSDTGERWRVFATRMAFDRVGLVAVPLSEVTAAIR
                     RLVVLEALAGIGLVTALGIGSWLILRRGLRPLENIASTASSITAGDLRHGSITARVAP
                     ANTQSEVGQLGLAIDTMLDEIDVALRERDATEHKLRQFLADASHELRTPLTSIQGFAE
                     LFRLGAADDPEQLAVVLGRIESESQRMSTLVEELLLLARLDQMRPIERRPVDLTVLGA
                     DACTDARATDPDRAVTLDASTSVVVAGDAMHLRQAIANLVTNALRHTPDSTPIEVAVT
                     SDGATATLSVRDHGAGLDPEALERVFDRFWQADSARVGTGTGLGLAIVHAIAAEHGGG
                     VEARNHPDGGAEFLIRIPVAPAGDPVPAPAPAPASNANPSEST"
     gene            1480..2397
                     /locus_tag="PLP95_04190"
     CDS             1480..2397
                     /locus_tag="PLP95_04190"
                     /inference="COORDINATES: protein motif:HMM:NF012517.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HPB45033.1"
                     /translation="MKIRIGVGTGVGQTLEQPSQFGEVVDLVESLGFDSLWVSDRVVG
                     ESLDPTVSLAIAAGRTSRIKLGANVWVLPGRDPYLMAKQLASLSLLSDGRLLPAFGLG
                     SPHPADREPFGVAKGRRVAMFEEGLSVVRALWAGESIPHPHGGSALRLKPRPAEPLDI
                     WFGARSEAALARTGRLADGWIGSFQTPRRARHCREVVLRGAEDAGRTFDLEHFGMTIM
                     YARHQRTELGRFILSVLGGDDRSDSTDGGGDGYEAACYPCGADELVAVLNQHIDAGIS
                     KFVLSPADAPEDWGDELRWLREVTGPLET"
     gene            complement(2435..3388)
                     /locus_tag="PLP95_04195"
     CDS             complement(2435..3388)
                     /locus_tag="PLP95_04195"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HPB45034.1"
                     /translation="MGIPLPTLAADEIHPNAPAWFRRALAVPGTIEQVEVDGVSVEYL
                     AWGERGRPGLVFVHGGGAHAYWWAPVAARFAGECRVAAITLSGHGDSGRREVYTLESW
                     TDEVAAVIEDADMAPHPVAVGHSMGGMVSIATAARHGHRLGGVIIVDSPVDRPDPEVR
                     SFHLREAFGKKRVYPSVDAAVARFRTIPAQEHYLDFVLDYVARRSVRAVEGGFAWKFD
                     DRIFEQLGTAVRSVALPYLPHIACRVALLRSEYGLINPEIRERMFEAMGRRTPIIELP
                     EAGHHPMLDVPQILVTALRALIADWDFSIPSVVPPPPLSVD"
     gene            3430..4083
                     /locus_tag="PLP95_04200"
     CDS             3430..4083
                     /locus_tag="PLP95_04200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020378189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HPB45035.1"
                     /translation="MDVTVFLVDDHEIVRRGVRTLIDNESDLTVIGEASTVVEALAQI
                     PEAGPRVALLDLHLPDGTGVELCRAVREQCPDIACLILTSYEDDGAIVEATAAGAAGY
                     LLKQIRGGEILSAIRAAAQGQTVLDAARASGGHRAPRRDQGEAELDARAESLSPQERR
                     ILDHLATGASNREIADELGLAEKTVKNYVSGLLSKMGMTRRTEAAVYAVRLAERRRG"
     gene            complement(4103..4669)
                     /locus_tag="PLP95_04205"
     CDS             complement(4103..4669)
                     /locus_tag="PLP95_04205"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HPB45036.1"
                     /translation="MTAVQRPPLTRRRQATFDGLLDSARRLLDEDGYDGLTVRAVALA
                     AGVTHTTAYSFVPSKAQLVAELYLREMERLDPLVLSPSASPADRAAEAIEGPALLFSG
                     EPAVGHAVQVALMMGDGDPALMQLRERIAAITLGRLRQALPGVDAEVIGVIFAAYNGA
                     MVTVGMGHGTHADIVRTVRRLVELIGLS"
     gene            complement(4666..>5367)
                     /locus_tag="PLP95_04210"
     CDS             complement(4666..>5367)
                     /locus_tag="PLP95_04210"
                     /inference="COORDINATES: protein motif:HMM:NF013530.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HPB45037.1"
                     /translation="VLEAAADNGVRRVVYTSSLAVFLPTPDEVITDASALAEPLSAYG
                     RSKRDAELLVRAAQDDGRDIVTLYPGGVYGPISTTLDNGFAAIIGAMNFAMLCPPGGM
                     GVIDVRDVADLIVAALERPQGANRYLCGGHFVTWMQWTEALRQAAGTEVSAVEVTAEA
                     MIEMGRDFDRQREAGAPSSPLSEEAAIIMCAGRPTDDSRTLAELNMSYRPVVETFADV
                     VEYLRGLGELPTIDR"
BASE COUNT          862 a         1903 c         1790 g          812 t
ORIGIN      
        1 cgacgacatg acgttgcgcc tccgactcac cctcgccatc gtcgccctga cctcggttgg
       61 cctcatcgtg ttctcggtcg tgctgacgag cttctatgcc gccagccagt acgaccgact
      121 cgacgaacgc ctcagcacca ccggcccgga ggcgatcaat cttcttgccg cggagatcgg
      181 cctcgaagcg ccgacacccg ggcgtctggg caccgggcgc caccgcgtgc tcggacccgg
      241 cctcgacgtc tcggtcgagc ttcgcgaccg caacggtgag gtgctcggat ctgcggttct
      301 gctcggcacg gagaacccgc cggagttccc cgcgacggtg aaccctgaac ggggcaccaa
      361 cctgttcttc aacgttcgaa gcgacaccgg ggaacgctgg cgggtcttcg ccacgaggat
      421 ggccttcgac cgcgtcgggc tcgtagcggt gccgctgtcg gaggtcaccg ctgccatccg
      481 cagactcgtc gtgctcgagg cgctggccgg aatcgggctg gtgacggcgc tcggcatcgg
      541 gtcgtggctg atcctgcgcc ggggactgcg cccgctcgaa aacatcgcgt cgaccgcgtc
      601 gtcgatcacg gccggcgatc tccgccatgg gtcgatcacg gcgcgggtcg cgccggccaa
      661 cacccaaagc gaagtcggac aactcgggct cgcaatcgac acgatgctcg acgagatcga
      721 tgtcgcgctc cgcgaacgcg acgccaccga acacaagttg cgccagtttc tcgcggatgc
      781 ctcccacgag ttgcgcaccc cgctgacctc gatccagggc ttcgccgaac ttttccgcct
      841 cggggcggcg gacgaccccg aacagctcgc ggtggtgctg ggccgcatcg agtcggaatc
      901 tcagcgcatg agcaccctgg tcgaggagtt gctgttgctg gctcgcctcg atcagatgcg
      961 cccgatcgaa cgccgcccgg tcgatctcac cgtgctcggc gccgacgcgt gcaccgacgc
     1021 acgggcgacc gaccctgatc gagcggtcac cctcgacgcc agcaccagcg tcgtggtggc
     1081 cggcgacgcc atgcacctgc gccaggcgat cgccaacctc gtcacgaatg cgctgcgcca
     1141 cacccccgac tcaacgccga tcgaggtcgc cgtcacctcc gacggggcaa cggccacgct
     1201 gagcgtgcga gaccacggcg ccggcctcga ccccgaggcg cttgagcggg tcttcgaccg
     1261 gttctggcag gccgacagcg ccagggtggg aacgggcacc ggactcgggc tggccatcgt
     1321 tcacgccatc gccgccgagc acggtggcgg tgtcgaggcg cgcaatcacc ccgacggcgg
     1381 tgccgaattc ctgatacgaa tcccggtagc cccggccggc gacccagtcc ccgccccagc
     1441 cccagccccg gcgtcgaacg ccaacccatc ggagtccaca tgaagatcag gatcggcgtc
     1501 ggaaccgggg tcggccagac cctcgagcag ccgagccagt tcggcgaggt tgtcgacctc
     1561 gtcgagtcgc tcgggttcga ctcgctgtgg gtgtcggacc gtgtcgtcgg cgagagcctc
     1621 gaccccacgg tttcgcttgc gatcgctgcc gggcgcacca gccgcatcaa actcggcgcc
     1681 aacgtgtggg tactgccggg ccgcgacccc tacctcatgg caaagcaact cgcctcgttg
     1741 tcgctgctga gcgacgggcg gctgctgccc gcattcgggc tcggctcacc gcatcccgcc
     1801 gatcgcgagc cgttcggggt tgcgaagggt cgccgcgtcg cgatgttcga agagggcctg
     1861 tccgtcgttc gggcgctgtg ggccggcgag tcgattcccc atccccatgg gggatccgcg
     1921 ctgcgactca agccgcgacc cgccgagccg ctcgacatct ggttcggtgc gagaagcgag
     1981 gccgcgctcg cacgaaccgg gcgcctggcc gacggttgga tcggcagctt tcaaaccccg
     2041 cggcgcgccc gtcactgtcg tgaggtggtg ttgcgtggcg ccgaggacgc cggccggacc
     2101 ttcgacctcg aacacttcgg catgacgatc atgtacgcgc gccaccagcg caccgaactc
     2161 ggcaggttca tcctgtccgt gctcggcggc gacgaccgca gcgacagcac cgacggcggc
     2221 ggcgacgggt acgaggccgc gtgctacccc tgcggcgccg acgaactcgt cgcggtgttg
     2281 aaccaacaca tcgacgccgg catctccaaa ttcgtcctgt cccctgccga tgcgcccgag
     2341 gactggggcg acgagttgcg ctggctgcgc gaggtcaccg gcccactcga aacctgagct
     2401 gcgaccgctc agtcgaaacc tgagctgcga ccgctcagtc caccgacagc ggcggtggcg
     2461 gcaccaccga ggggatcgag aaatcccaat cggcgatcag cgcccgcagc gcagtcacga
     2521 ggatctgcgg cacgtcgagc atcgggtgat ggccggcttc gggcagttcg atgatcgggg
     2581 tgcgacgccc catcgcttcg aacatccgct cgcggatctc cgggttgatc agtccgtact
     2641 cggaacgcaa cagcgcgacg cgacaggcga tatgtggcag gtacggcagc gccacgctac
     2701 gaacggcggt gccgagctgc tcgaagatcc ggtcgtcgaa cttccaggcg aagccgccct
     2761 cgacggcgcg caccgaccgg cgcgcgacgt agtcgagcac gaagtccaga tagtgctcct
     2821 gggccggaat cgtgcgaaat cgagccacgg cagcgtcgac ggagggatag acccgcttct
     2881 tgccgaacgc ttcgcgcagg tggaacgaac gcacctccgg gtcggggcgg tcgaccgggg
     2941 aatcgacgat gatcacccca ccgaggcggt ggccgtgacg cgctgcggtg gcgatcgaga
     3001 ccatgccgcc catgctgtgg cccaccgcca ccggatgtgg cgccatgtcc gcgtcctcga
     3061 tgaccgcggc gacctcatcg gtccagctct ccaaggtgta gacctcgcga cggccgctgt
     3121 cgccgtggcc cgacagggtg atcgccgcga cacgacattc gccggcgaac cgtgcggcga
     3181 ccggcgccca ccagtaggca tgggctccgc cgccgtgcac gaacaccagc cccggccggc
     3241 cgcgctcccc ccacgccagg tactccaccg agacgccgtc aacctccacc tgttcgatcg
     3301 ttccgggcac cgccagcgcc cgtcggaacc aggcgggggc gttgggatgg atctcatcgg
     3361 cggcgagggt cggcagcggg atgcccatgt cgtggcaccg tagccgactc tgtcggccac
     3421 actggtgtga tggacgtgac ggtattcctc gtcgacgatc acgagatcgt ccggcgaggt
     3481 gtgcgaaccc tcatcgacaa cgaatccgac ctgacggtga tcggtgaggc atcgaccgtg
     3541 gtcgaggcgc tcgcgcagat ccccgaggcc ggcccccgcg tggcgctgtt ggacctccac
     3601 ctgcccgacg gcaccggtgt cgagctctgc cgcgccgtac gcgagcaatg ccccgacatc
     3661 gcctgtctca tcctcacctc ctatgaggac gatggggcga tcgtcgaggc aaccgcggcg
     3721 ggcgctgcgg ggtacctcct caagcagatc cgcggcggcg agattctgtc ggccatccgc
     3781 gccgcggctc agggccaaac ggtgctcgat gcggcgcgcg cttcgggtgg ccatcgggcg
     3841 cctcgacgag accagggcga agccgaactc gatgcccgag cggagtcgct gtcgcctcag
     3901 gagcggcgca tcctcgatca cctcgcgacc ggagcctcca accgggaaat cgccgacgaa
     3961 ctcggtctcg ccgagaagac ggtgaagaac tacgtgtcgg gcctgttgtc gaagatgggc
     4021 atgacccgcc gcaccgaggc cgcggtctac gccgttcgtc tcgccgagcg tcgccgcggc
     4081 tgagcgcctc aggtgagcat ggtcagctca ggccgatgag ctcgacgagt cgccgcaccg
     4141 tgcgcacgat gtcggcgtgg gttccgtgcc ccatcccgac ggtgaccatg gcgccgttgt
     4201 aggcggcgaa gatcaccccg atcacctcgg cgtccactcc gggcaacgcc tggcgaagcc
     4261 gaccgagggt gatcgccgcg atccgttcac gcagttgcat gagcgctggg tcgccgtcgc
     4321 ccatcatcaa ggcgacctgc acggcgtggc cgaccgcggg ttcgccggag aacaacaacg
     4381 cgggcccctc gatcgcctct gctgcccggt cggccggcga ggccgacggt gagagcacca
     4441 gcgggtcgag gcgttccatc tcgcgcaggt acaactcggc gacgagttgc gccttggatg
     4501 gcacgaagga ataggccgtc gtgtgggtca cccccgcggc gagtgcaacc gcacgtaccg
     4561 tcagcccgtc gtagccgtct tcgtcgagga gccgccgagc cgagtcgagg aggccgtcga
     4621 aggtcgcctg gcgccgtcgg gtcagcggtg gccgttgcac tgcggtcatc ggtcgattgt
     4681 cggaagttcg ccgagcccgc gcaagtactc gaccacatct gcgaaggtct cgacgaccgg
     4741 ccggtacgac atgttgagct cggcaagggt tcgcgagtcg tcggtcgggc ggcccgcgca
     4801 catgatgatc gccgcctcct ccgaaagcgg actcgacggt gcgccggcct ctcgctgacg
     4861 gtcgaagtcc ctgcccatct cgatcatcgc ctccgcggtg acctcgaccg cggacacctc
     4921 ggtgccggcg gcctgtcgaa gcgcctcggt ccattgcatc caggtcacga agtgcccacc
     4981 gcacaggtac cggttcgctc cttggggacg ctcgagcgcg gccacgatca ggtcggccac
     5041 gtcgcgcacg tcgatcactc ccattccgcc gggcggacac aacatggcaa agttcatcgc
     5101 cccgatgatc gccgcgaacc cgttgtcgag ggtcgtcgaa atcgggccgt agaccccgcc
     5161 cggatacagg gtgacgatgt cgcggccgtc gtcctgggcg gcgcgaacca gcagttccgc
     5221 gtcgcgtttg gaccgcccgt aggccgacag cggttcagcc aaggcgctgg cgtcggtgat
     5281 gacctcgtcg ggggtgggca ggaacaccgc gaggctcgag gtgtacacga cccgacgcac
     5341 cccgttgtcg gcagccgcct ccaacac
//
LOCUS       DAOOMA010000104         5334 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_18537_length_5334_cov_4.083917, whole genome shotgun sequence.
ACCESSION   DAOOMA010000104 DAOOMA010000000
VERSION     DAOOMA010000104.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 5334)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5334)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5334
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18537_length_5334_cov_4.083917"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..273
                     /locus_tag="PLP95_04215"
     CDS             <1..273
                     /locus_tag="PLP95_04215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPB45038.1"
                     /translation="AVARALMTRPTIVFGDEPTGNLDSRSSAEVLQFMRTTVDQAAQT
                     VVIVTHDPVAASYTDRVVFMADGRIVDSIESPTTESVIDHMLQFGS"
     gene            279..2777
                     /locus_tag="PLP95_04220"
     CDS             279..2777
                     /locus_tag="PLP95_04220"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HPB45039.1"
                     /translation="MFSIAMKTLRANLSRFIATLVAIAIGVAFLVAGNMLTTSIRNSL
                     GGEIDRQYEHVDAAVTLRNEELAGSGLMSGAGIDKVLVETISALPHVEAAIGDISGMS
                     RFAEDAFADDLSIMDMQGLTVRPWYDNDLNAVTIIEGRPPKAEGEVTFDRGTMKDREL
                     SLGDTVSLTSLDGAVDVTVVGVTSFGTTDSVDSTGTVMAAPDWAFTLGANAEQTFTRV
                     LVQSDGTVSQGEFAESIRVTLPDGFTAVTGDTFRESMRSVLNEVLNVLGPVLTGFSLL
                     ALFVCGFVIFNTFSVVVQQRTRELALMRAVAATPRQIRRSLRLEGFGIGLIGSVIGLL
                     LGTLLTVGLTAVLNAFDLDLPPASIVITPGIVITAFAAGIIVTMVSVLIPAWRAGRTA
                     PVEAMREAAIERNRLGRIRLGAAVVLFLAGVGLCLTRSGWFVGIGAFLLVIGVFLSGP
                     ALTVLVAWATRPLVSLTGTPGRLSSENLSRNPKRTATTMNALVIGVMLVTLVSIAGNS
                     LKSTVLSYVEDQQSTDVYVGAMTGAISPEMVEEIEAVDGVETSVRIRQYNVTIDGAVG
                     VVSTTDDLIALSRLGITPVSMSWDELGDRAVSLPMDANSMTLGPKTITNALGTSITVE
                     LADYLESTFDAGSLGYVLSPELFDEIAPADNGVNYMMIAFEPTRKSEATREIKEITQG
                     LGNIFVFEGNIFGRIIESVFNFLINAVNGLLGMSVVIALIGLVNTLSLSIFERRRELG
                     LLRAVGMTRREVRRMVRFEAVQMSLLGTIIGLASGALVAWLLLRATELADIAFGWKQL
                     AGIFVLGIVLGVVAAIAPTRRASRLDILDAVKVE"
     gene            complement(2850..3515)
                     /locus_tag="PLP95_04225"
     CDS             complement(2850..3515)
                     /locus_tag="PLP95_04225"
                     /inference="COORDINATES: protein motif:HMM:NF022182.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjN domain-containing protein"
                     /protein_id="HPB45040.1"
                     /translation="MGDNENDAIDLEHSNGWLAFEMLGTLLEEEEFHPEQLSGTTAYR
                     FGVSGTNGQFRVVAHCNVELEQLYVYVLTDVRVPEPRRGAVAELIARANYGMRIGNFE
                     LDMRDGEVRYKSSLDFEHNELTPSLVHNAMMPALATMDRYFAVVMSVAFGSVSPADAI
                     TVLEPAPQVPADEFPAEGFDEEGFDDDELLDDEFPEFELGEADVSNDASTDPAEDDHP
                     AED"
     gene            complement(3630..>5334)
                     /gene="uvrB"
                     /locus_tag="PLP95_04230"
     CDS             complement(3630..>5334)
                     /gene="uvrB"
                     /locus_tag="PLP95_04230"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HPB45041.1"
                     /translation="EKDSSVNDEIDRLRHAATSALLTRRDVIVVASVSCIYGLGSPAE
                     YAARMVVMRVGEEHEQRNLLRRLVAMRYERNEMNLTRGKFRVKGDTLEVHPAYDETAV
                     RIEFFGDEIEAISVFDPLTGERLRQLDEFILFPATHYTTSDDRMTAAIGRIEDELQQR
                     LAYFEREGKLLEAQRLRMRTQFDLENMAEMGFCNGIENYSAPIDGRAPGEPPFTLLDF
                     FPDDYLTIIDESHVTIPQLHGQFEGDRSRKQTLIEHGFRLPSAADNRPLRFEEWVERA
                     GQTIFLSATPGPWEREHSGAIVEQVVRPTGLIDPVVEVRGTKGQIDDLLAEINKRAEL
                     GERTLVTTLTKKMAEDLTDYLLEMGVRVRYLHSEVDTIARIELIRDLRLGEFDVLVGI
                     NLLREGLDIPEVSLMAILDADKEGFLRSESSLIQMIGRAARNSEGVVVMYADSMTKSM
                     DRAIGETNRRRELQLAYNAEHGIVPQTIRKQVRDIVADLRGRGDTPVPGKDRRRQRAA
                     TSTLREEFGSLAPQDLGTLIETLEMEMNEASADLRFEYAARLRDEIKELKKELRDAGA
                     A"
BASE COUNT          858 a         1808 c         1660 g         1008 t
ORIGIN      
        1 gcggtcgcca gggcgctcat gacccggccc acgatcgtgt tcggcgacga gcccaccggc
       61 aacctcgact cgcgttcgag cgccgaggtg ttgcagttca tgcgcacgac cgtcgatcag
      121 gccgcccaga ccgtggtgat cgtcacccac gatccggtcg ccgccagcta caccgatcgc
      181 gtcgtgttca tggccgacgg ccgcatcgtc gacagcatcg aatctcccac caccgaatcc
      241 gtcatcgacc acatgttgca gttcgggagc tgagcgccgt gttctccatc gccatgaaga
      301 ccctccgcgc caacctgtcg cggttcatcg ccaccctcgt cgccatcgcc atcggggtgg
      361 cgttcctcgt cgccggcaac atgttgacga cctcgatccg caactccctc ggcggagaaa
      421 tcgaccggca gtacgaacac gtcgacgccg cggtgaccct gcgcaacgag gaactcgccg
      481 ggtcgggact catgtcgggt gcgggcatcg acaaggtcct cgtcgagacc atctccgcgc
      541 tcccccacgt cgaggcggcg atcggcgaca tcagcgggat gtcgcgcttc gccgaggacg
      601 cgttcgccga cgacctgtcg atcatggaca tgcaggggct caccgttcgc ccgtggtacg
      661 acaacgacct gaacgcggtg accatcatcg aggggcgccc accgaaggcg gaaggcgagg
      721 tcaccttcga ccgcggaacg atgaaggatc gcgaactgtc ccttggcgac accgtgtcgc
      781 tgacctccct cgacggagcc gtcgacgtga ccgtcgtcgg ggtcaccagc ttcggcacca
      841 ccgactcggt cgattccacc ggcacggtga tggccgcccc cgactgggca ttcaccctcg
      901 gagccaacgc cgaacagacc ttcacccgcg tgctcgtgca gagcgacggc accgtgtctc
      961 agggcgaatt cgccgagtcg atccgtgtca ccctccccga cggattcacc gccgtcaccg
     1021 gggacacgtt ccgcgaaagc atgcgaagcg tcctcaacga ggtgctcaat gtgctcgggc
     1081 ccgtactcac cgggttctcg ctgttggcgc tgttcgtctg cggattcgtg atcttcaaca
     1141 ccttctcggt cgtcgttcag caacgcaccc gcgaactcgc gttgatgcgc gccgtcgcgg
     1201 ccaccccacg ccagattcga cgttccctgc gactcgaagg attcggcatc ggcctgatcg
     1261 gatcggtcat cggactactc ctcggcacgt tgctcacggt ggggctcacc gccgtgctca
     1321 acgcgttcga cctcgacctt ccaccggcgt cgatcgtcat cacccccggg atcgtcatca
     1381 ccgcgttcgc cgccgggatc atcgtcacca tggtgtcggt gctgatcccc gcctggcggg
     1441 ccggacgaac cgccccggtc gaagccatgc gcgaggccgc catcgagcgc aaccggctcg
     1501 gtcgaatccg cctcggcgcc gccgtcgtgt tgttcctcgc cggcgtcggc ctgtgcctca
     1561 cccgctccgg gtggttcgtc ggcatcggtg cgttccttct ggtgatcggc gtgttcctct
     1621 ccggccccgc cctgaccgtg ctcgtggcgt gggcaacccg cccgctcgtc tcgctcaccg
     1681 gaacgccggg tcgactgagc tcggagaacc tgtcgcgaaa ccccaaacgc accgccacca
     1741 cgatgaacgc gctcgtgatc ggggtcatgc tcgtcacgct ggtgtcgatc gccggcaact
     1801 cgctcaaatc gaccgtgctg tcctacgtcg aggaccagca gagcaccgac gtctacgtcg
     1861 gggcgatgac cggggcgatc agcccggaga tggtcgagga gatcgaagcg gtcgacggcg
     1921 tcgaaacctc ggttcgaatc cggcagtaca acgtcacgat cgacggggcg gtcggcgtcg
     1981 tgagcaccac cgacgacctc atcgccctct cgcggctcgg catcacgccg gtgtcgatga
     2041 gctgggacga gctcggcgac cgagccgtgt cgttgccgat ggacgcgaac tcgatgacgc
     2101 tcggccccaa gacgatcacg aacgcgctcg gcacctccat caccgtggag cttgccgact
     2161 atctggaaag taccttcgac gccgggtcgc tcggctacgt gttgtcaccc gaactgttcg
     2221 atgagatcgc tcccgccgac aacggcgtga actacatgat gatcgccttc gaaccgaccc
     2281 ggaagtccga ggcgacccgc gagatcaagg agatcaccca agggctcggc aacatcttcg
     2341 tgttcgaggg caacatcttc gggcggatca tcgagtcggt cttcaatttc ctgatcaacg
     2401 ctgtgaacgg cctgctcggg atgagcgtag tgatcgccct gatcggcctg gtcaacacgc
     2461 tgtcgttgtc gatcttcgaa cgccgccgcg aactcggact gctgcgcgcc gtgggaatga
     2521 cccgacgcga ggtgcgtcgg atggtgcgtt tcgaggccgt gcagatgtcg ctgctcggca
     2581 cgatcatcgg cctcgcaagt ggcgcgcttg tcgcctggct gttgttgcga gccaccgagt
     2641 tggccgacat cgcattcggg tggaaacagc ttgcgggcat cttcgtgctc gggatcgtcc
     2701 tgggtgtcgt cgctgcgatc gcccccaccc gccgggcctc gcgtctcgac atcctcgacg
     2761 cggtcaaggt cgaatagccg acagacgtga ccgcctggcg acgcgtccgc ctggcggtcg
     2821 gtcgtcctgg cggtcggtct tcctggcggt cagtcctcgg ccgggtgatc gtcctcggcc
     2881 gggtcggtcg acgcgtcgtt cgacacgtcg gcctctccga gttcgaactc cgggaactcg
     2941 tcgtccagaa gctcgtcgtc gtcgaagccc tcctcgtcga acccctcggc tgggaactca
     3001 tccgcgggga cctggggtgc aggctccaga acggtgattg cgtcggccgg ggagacggac
     3061 ccgaacgcaa cgctcatcac cacggcgaag tagcgatcca tcgtggcgag cgccggcatc
     3121 atcgcgttgt gcaccaggct cggcgtcaac tcgttgtgtt cgaagtcgag gctcgacttg
     3181 taccggacct cgccgtcgcg catgtcgagt tcgaaattgc caatacgcat gccgtagttc
     3241 gcccgagcga tcagttcggc caccgccccc cgacgcggtt cgggtactcg aacgtcggtg
     3301 agcacgtaga cgtagagctg ttcgagttcc acgttgcagt gcgcgaccac tcggaactgt
     3361 ccgttggttc cggaaacccc gaatcggtac gccgtcgttc cgctgagttg ttcgggatgg
     3421 aactcctctt cctcaagcag cgtgcccagc atctcgaacg cgagccagcc attgctgtgt
     3481 tcgagatcga tggcgtcgtt ttcgttgtcg cccatgacgg caggttatgc cccgacgctg
     3541 cgtttcgtcg ttcggatatt cagcgctctc acagcacctc gctgcgtttc ggattctgac
     3601 ccaccaactc gccgcccaac cctggatgct caggccgccc cggcatcgcg caactccttt
     3661 ttgagttcct tgatctcgtc tcgcagccgt gccgcgtact cgaagcgcag gtcggccgag
     3721 gcctcgttca tctccatctc gagggtttcg atgagcgtgc cgaggtcctg tggggccagc
     3781 gacccgaact cctcgcgcag ggtcgaggtt gcggcgcgct ggcgacggcg gtcctttccc
     3841 ggcaccggcg tgtcgcccct gccgcgaaga tcggccacga tatcgcgcac ctgtttgcga
     3901 atggtctggg gcacgatgcc gtgttcggcg ttgtaggcga gttgcagctc gcggcgacgg
     3961 ttcgtttcgc cgatcgcccg gtccatcgac ttggtcatcg aatccgcata catgaccacg
     4021 acgccctcgg agtttctcgc cgcgcgaccg atcatctgga tgagcgagga ctccgatcga
     4081 aggaagccct ccttgtcggc gtcgaggatg gccatcagcg acacctcggg aatgtcgagt
     4141 ccctcgcgca gcaggttgat gccgaccagc acgtcgaact cgccgaggcg caggtcgcgg
     4201 atgagttcga ttcgggcgat cgtgtcgacc tcggagtgca ggtagcgcac ccgcaccccc
     4261 atctccaaca gatagtcggt gaggtcctcg gccatcttct ttgtcagcgt cgtgaccaag
     4321 gtccgctcac cgagttcggc ccgcttgttg atttcggcga gcaggtcgtc gatctgcccc
     4381 ttcgtcccgc gcacctcgac gaccggatcg atcaggcccg tcgggcgcac gacctgttcc
     4441 acgattgccc cggagtgctc gcgctcccac ggccccggag tggccgacaa gaagatggtc
     4501 tggccggccc gctccaccca ttcctcgaac cgcagcgggc gattgtcggc ggccgagggc
     4561 agccggaaac cgtgctcgat cagcgtctgc ttgcggcttc gatctccctc gaactggccg
     4621 tggagttgag gaatggtgac gtgactctcg tcgatgatcg tcaggtagtc gtcggggaag
     4681 aaatcgagca gcgtgaacgg cggctcgccg ggggcgcgac cgtcgatcgg ggccgagtag
     4741 ttctcgatcc cgttacagaa ccccatctcc gccatgttct cgaggtcgaa ctgggtgcgc
     4801 atccgcaggc gctgggcctc gagcagcttt ccttcccgct cgaaatacgc cagccgctgc
     4861 tgcaactcgt cttcgatccg gccgatggcc gcggtcatcc ggtcgtcgga ggtcgtgtag
     4921 tgggtggctg gaaagaggat gaactcgtcg agctggcgca gccgttctcc ggtgagcggg
     4981 tcgaacaccg agatcgcctc gatctcgtcg ccgaagaact cgatacggac cgccgtttcg
     5041 tcatacgcgg ggtgaacctc gagggtgtcg cccttcaccc ggaacttgcc gcgggtcagg
     5101 ttcatctcgt tgcgctcata acgcatggcc acgagccggc gcagcaggtt ccgctgctcg
     5161 tgctcctcgc cgacgcgcat gaccaccatt ctcgcggcat attcggcggg tgagccgagg
     5221 ccgtagatgc acgaaacgga cgcgacgacg atcacgtcgc gccgcgtcag cagtgccgag
     5281 gtggcggcgt ggcgcaaccg gtcgatttcg tcgttcaccg aagagtcttt ttcg
//
LOCUS       DAOOMA010000105         5327 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_18567_length_5327_cov_3.805197, whole genome shotgun sequence.
ACCESSION   DAOOMA010000105 DAOOMA010000000
VERSION     DAOOMA010000105.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 5327)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5327)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5327
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18567_length_5327_cov_3.805197"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..447
                     /locus_tag="PLP95_04235"
     CDS             <1..447
                     /locus_tag="PLP95_04235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB45042.1"
                     /translation="RTPPTTTTIDRAEELVAIFEELQSTGSGWLNIVPELPEGTEVPA
                     TPNSLAIFSRRGPVVPLATWTTTEPTELGLLHGTSQRARFALADTVAAIPDGWRVLAD
                     HPRKGLVVVPGAGLGHRVVARWLLDALTVLCLPPHTGRFVVARYPG"
     gene            complement(467..1249)
                     /locus_tag="PLP95_04240"
     CDS             complement(467..1249)
                     /locus_tag="PLP95_04240"
                     /inference="COORDINATES: protein motif:HMM:NF012596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HPB45043.1"
                     /translation="MLTILDDARVRLLTLDRPDALNSFNEALYDALAEALIDAANDDS
                     VAVVVITGSGRAFSAGQDLLEMGERIANPDFVEGAHGFPGCVDQLIGFPKPLLLAING
                     LGLGIGTTIIGFADLAFMSTDAKLKCPFTSLGVAPEAASSLLFPRLMGHQNAAWVLLS
                     SEWILAPEAKELGLVWKITSPEDLLEVTMDHARRLAAMPISSLQAVKATMTAGRAEET
                     RAARDREDAQFVRLMGGPANMEALAAFAEKRPADFTNLAPGW"
     gene            1300..2394
                     /gene="menC"
                     /locus_tag="PLP95_04245"
     CDS             1300..2394
                     /gene="menC"
                     /locus_tag="PLP95_04245"
                     /EC_number="4.2.1.113"
                     /inference="COORDINATES: protein motif:HMM:TIGR01928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate synthase"
                     /protein_id="HPB45044.1"
                     /translation="MRISTVEIIPLRLPLLRPWHTIGGLMTEREVLMVRVRTDGADGW
                     GECSAFATPFYTAEFIASAHIVLRDRLAPLLTAAESVTSEGLGALFAPVAGHRMAKSA
                     LEMAVLDAELRTAGESFAEFLGSEVESVNAGVSIGFAESPSDLVDTVAEFAANGYRRV
                     KLKVAPGRDLDHIEAVREQFPAMSLQIDANGAYVRADVEHLAKFDGFGLTLIEQPLAS
                     DDLVGHAAVAKRLHTSICLDESLVSLRSVETALAVGACDVVNLKPSRVGGYHEALRIH
                     DWCFERGVGMWVGGMLETGLARAANIALASLPGFTLTGDLSGSDRFYADDIITEPLTV
                     TNGALAVPQGHGFGVDIDLDAIKRFRLSSS"
     gene            complement(2366..2914)
                     /locus_tag="PLP95_04250"
     CDS             complement(2366..2914)
                     /locus_tag="PLP95_04250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB45045.1"
                     /translation="MLGAEFVDEDDAMNVAAHAALIDPDRFQFAEQAERDGVFAAPAD
                     GAERSATRVIDGACIFLNRAGFAGGAGCALHLGALDYEDAPHEWKPLVCWQVPFQLDR
                     VTEPGSSVEVSILRRKRRVDFSSTGDEADAVAWICTEDPAAYGEVTPVLLRQREEFED
                     WLGTDVVEHLAERLSSTTAGNA"
     gene            complement(2875..5067)
                     /locus_tag="PLP95_04255"
     CDS             complement(2875..5067)
                     /locus_tag="PLP95_04255"
                     /inference="COORDINATES: protein motif:HMM:NF016612.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neutral/alkaline non-lysosomal ceramidase
                     N-terminal domain-containing protein"
                     /protein_id="HPB45046.1"
                     /translation="MSGSALDSVAHLRSGRASFEVKPAAPVRLDHVDGVLAGAASVDI
                     TPPPGMPKAGYSSNAHDGEGFRSRLRARVVHLRAGRSSLAIVHCDLLGGSSVLQYLVA
                     DAISAQCDVPLAGLMIGATHTHAGAGQFNGTDFYNRFASNRSGFDPAWSAFLVERIAS
                     AVIEATQTRRPAKVGFGSIDVWGATRNRSLDPHVTNATVRDKRQEPQRKFAAINPQLR
                     LLRVDDAADSTPIGASVIFSVHGTGISQHSREYNADVWAYLVDELAHHIESSRGAGGV
                     VGAIEGTHADVAPALYPNSAGYRDTKRVGRAIGHKAAELYDSLGDRLSDSVELGCAFE
                     EVSLVGGARRRANGTVAQLPNRPAVGAALAAGAMENLTPVIHRLPLFRAGMPRRFLKN
                     GPQGAKHVIGGRLQPLVLPLKAFPRSIPVQVLRIDSTAVVGLPFEVTVESGRRITAAV
                     LDAVAGPDEGLIDDVVVSSVANEYCGYVATAEEYSRQFYEGGHTLYGPNTERFLAACA
                     ADLASALAAGGDVAVQRTPPTRSWNLRVHRYLIESTTVQRSPQVVVEPQFHDYTDRID
                     GAWEVIWSDAEPGALCWHSTIVRVEVSRDGGDTWAPARHDGRRVDDGGTEMEVRHLGL
                     FDGAHRYAARWYDPAFGDDRLHRFVIVDADGSVLLSTEGFNGSPRSDGTSSSRSGANG
                     GASISARSGRDGVAGGGRGAAASATKPTPPTPWGVACSGRNSSTRTTP"
     gene            complement(5064..>5327)
                     /locus_tag="PLP95_04260"
     CDS             complement(5064..>5327)
                     /locus_tag="PLP95_04260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB45047.1"
                     /translation="VADPLYCGIRGAALSAAMAMGEVRPDEVRDLVSIKATYRPNALH
                     TAIYDRHYKEFKGLYKRQKSFFRRMNSAAKSAAKSAAKSAAKS"
BASE COUNT          880 a         1891 c         1687 g          869 t
ORIGIN      
        1 cgaacgccac cgacgaccac cacgatcgac cgcgccgagg agttggtcgc catcttcgag
       61 gaactccagt caaccggttc cggctggctc aacatcgtcc ccgaactccc cgagggcacc
      121 gaagtcccgg cgacaccgaa ctcgctcgcg atcttttccc ggcgaggccc ggtggtcccc
      181 ctcgccacct ggaccacgac cgagccgacc gaattggggc tcctccacgg cacctcccag
      241 cgcgcgaggt tcgcgctcgc cgacaccgtc gccgccatcc ccgacggctg gcgggtcctg
      301 gccgaccacc cgcgcaaggg cctcgtcgtc gtgcccggtg ccggactcgg acatcgcgtc
      361 gtggcccgat ggctactcga cgcgctcacg gtgctgtgcc tgcccccgca cacgggacgc
      421 ttcgtcgtcg cccgctaccc gggctgagtt gcgcggggcc tggctctcac cacccaggtg
      481 ccaggttggt gaagtcggcg ggacgctttt cggcgaacgc cgccaacgct tccatgttcg
      541 ccgggccgcc catgagtctg acgaactgtg cgtcttcacg gtctcgggcg gcgcgggtct
      601 cctcggctcg gccggcggtc atcgtggcct tcaccgcctg cagcgaactg atcggcatcg
      661 ccgcgagccg ccgagcgtga tccatcgtca cctccaacag gtcttccggc gaggtgatct
      721 tccacacgag gccgagttcc ttcgcctccg gggcgaggat ccattccgag ctcaacagca
      781 cccaggccgc gttctgatgc cccatcaggc gcgggaacag cagcgaggac gccgcttctg
      841 gagcgacccc gagcgaagtg aagggacact tcagcttggc gtcggtcgac atgaacgcca
      901 ggtccgcgaa cccgatgatc gtcgtgccga ttcccaggcc caggccgttg atcgccagca
      961 acaacggctt cggaaagccg atgagctgat cgacgcaacc cggaaacccg tgtgcaccct
     1021 cgacgaaatc ggggttggcg atccgttcgc ccatctccaa caggtcctgc cccgccgaaa
     1081 acgctcggcc ggaccccgtg atgacgacga cggccaccga atcatcgttc gccgcatcga
     1141 tgagtgcctc ggccagcgcg tcgtacagcg cctcgttgaa ggaattgagg gcgtcggggc
     1201 gatcgagggt gagcagacgg acacgagcat cgtccaaaat cgtcaacatg gaacctgttc
     1261 tagttcgttt cgcggcaaag gtgcgctaaa cccgtgggaa tgcgtatctc gacggtggaa
     1321 atcatcccgc ttcgactccc gcttcttcgg ccgtggcaca cgatcggcgg actcatgacc
     1381 gaacgtgagg tgctgatggt gcgcgtccgc accgacggtg ccgacggctg gggcgaatgc
     1441 tcggccttcg ccaccccgtt ctacacagcg gagttcatcg cgtcggccca catcgtcctt
     1501 cgcgaccgcc ttgcgccgct gctgaccgct gcggaatcgg tgacctccga aggtctcggt
     1561 gccttgttcg cccccgtcgc cggccaccgc atggccaagt ccgcgctcga aatggcggtg
     1621 ctcgacgccg agctgcgcac cgcaggtgag tccttcgccg aattcctcgg cagcgaggtc
     1681 gagtcggtga acgccggggt ctcgatcggc ttcgccgagt ccccctccga cctggtcgac
     1741 accgtcgccg agttcgccgc caacgggtac cggcgggtca agctgaaggt tgcgccgggc
     1801 cgcgacctcg accacatcga ggcggtgcgc gaacagttcc ccgccatgtc cctgcagatc
     1861 gacgccaacg gagcatatgt gcgcgccgac gtcgaacacc tcgcgaagtt cgacgggttc
     1921 ggcctcacgc tcatcgaaca accactcgct tcggacgacc tcgtcggcca cgccgccgtc
     1981 gccaaacgac tgcacacgtc gatctgcctc gacgaatcgc tcgtgtcgtt gcggtcggtg
     2041 gaaaccgccc tcgccgtcgg agcgtgcgac gtcgtgaacc tcaagccctc acgcgtcggc
     2101 ggctaccacg aggcgttgcg catccacgac tggtgtttcg agcgcggcgt cggcatgtgg
     2161 gtcggcggca tgttggaaac cggactcgca cgggccgcga acatcgcgct ggcctcgctt
     2221 cccggattca cgctcaccgg cgacctgtcg ggcagcgacc gcttctatgc cgacgacatc
     2281 atcaccgagc cgctcacggt gaccaatggt gcactcgcag tcccccaggg tcacggtttc
     2341 ggcgtcgaca tcgatctcga cgccatcaag cgtttccggc tgtcgtcgag ctgagccgtt
     2401 cggcgaggtg ttcgacgaca tcggttccca gccagtcctc gaactcctcg cgttggcgaa
     2461 gcaacaccgg ggtcacctcc ccgtacgctg cggggtcctc ggtgcagatc cacgccacgg
     2521 cgtcggcctc gtcgccggtt gaggagaagt cgacgcgacg cttgcgccgc agaatcgaca
     2581 cctcgaccga cgacccgggt tcggtcaccc ggtcgagttg aaacggcacc tgccaacaca
     2641 ccaacggctt ccactcatgc ggtgcatctt cgtagtcgag cgcgcccaga tgaagagcac
     2701 atcccgcgcc acccgcgaac ccggcccggt tcaagaagat gcatgcgcca tcgatcaccc
     2761 gcgtcgccga tcgctcagcc ccgtcggccg gcgccgcaaa gacgccgtcc cgttcggcct
     2821 gttcggcgaa ctggaatcgg tcggggtcga tcagcgcggc gtgggccgcc acgttcatgg
     2881 cgtcgtcctc gtcgacgaat tccgccccga gcacgcaaca ccccagggtg tgggtggggt
     2941 cggcttcgtc gccgatgccg cggcaccacg acccccaccc gcaactccat cccgacccga
     3001 gcgcgccgag atcgacgcgc caccgttcgc cccgctccga gacgaactcg tgccatcgct
     3061 cctcggggat ccgttgaatc cctcggtgct caacaggacc gagccgtcgg catcgacgat
     3121 cacgaagcgg tgcaaccggt cgtcgccgaa ggccgggtcg taccaacgcg ccgcgtaacg
     3181 gtgagccccg tcgaacagcc ccaggtgtcg aacctccatc tcggtaccac cgtcgtcgac
     3241 acgtcgaccg tcgtgacgag cgggcgccca cgtgtcgccg ccgtcgcggg acacctcgac
     3301 gcgaacgatc gtcgaatgcc aacacaacgc acctggttcg gcgtcgctcc agatgacctc
     3361 ccaggccccg tcgatccggt cggtgtagtc gtgaaattgc ggctccacga ccacctgggg
     3421 agatcgctga accgtcgtcg actcgatcag atatcggtgc acccgaaggt tccacgatcg
     3481 cgtcggcggt gtccgttgca ccgcgacgtc acctcccgcg gcgagggccg aggcaagatc
     3541 cgcggcgcag gccgcaagaa atcgctcggt gttgggtccg tacagggtgt ggccgccctc
     3601 atagaactgc cgtgagtatt cctcggcggt tgcgacatag ccgcagtact cgttcgccac
     3661 cgacgacacc accacgtcgt cgatcaggcc ctcatccggc ccggcgacgg cgtcgagcac
     3721 cgcagcggtg atacggcgcc ccgactcgac cgtgacctcg aagggcaacc cgaccaccgc
     3781 ggtcgaatcg atgcgcagca cctgcacggg aatcgatcgt gggaatgcct tgagcggcaa
     3841 caccagcggc tgcaatcgac cgccgatgac gtgtttggct ccctgcggac cgttcttcag
     3901 gaaccgtcgc ggcatgcccg ctcgaaacag cggaagccgg tggatcaccg gcgtgaggtt
     3961 ctccatcgcc cccgccgcca gcgctgcgcc caccgccggt cggttcggaa gctgggcgac
     4021 ggtaccgttg gcccgccgcc tggctccccc gacgagcgac acctcctcga aggcacaccc
     4081 caactcgacc gagtcgctca accgatcgcc gagtgagtcg tagagctccg cggccttgtg
     4141 cccgatcgcc cggcccaccc gcttggtgtc gcgatagccc gcactgttgg gatacagcgc
     4201 tggcgcgaca tcggcgtgag ttccctcgat ggccccgacg acaccgccgg caccgcggct
     4261 ggattcgatg tggtgggcca attcgtcgac gaggtaggcc cacacatccg cgttgtactc
     4321 gcgggaatgc tgggagatgc cggtgccgtg cacgctgaag atgaccgagg cgccgatcgg
     4381 ggtggaatct gcggcgtcat cgacgcgcag caggcgaagc tgcggattga tcgccgcgaa
     4441 cttgcgttgt ggctcctggc gcttgtctcg cactgtcgcg ttggtgacgt gtgggtcgag
     4501 cgaccggttc cgcgtggctc cccacacgtc gatcgagccg aacccaacct ttgccggccg
     4561 ccgcgtctgg gtggcctcga tgaccgccga tgcaatgcgc tcgacgagga acgcactcca
     4621 ggccggatcg aagccgctgc ggttggacgc gaaacggttg tagaagtccg ttccgttgaa
     4681 ctgcccggcc ccggcgtggg tgtgcgttgc gccgatcatg agcccggcga ggggcacgtc
     4741 gcactgcgcc gagatggcat cggccaccag gtactgcagc accgaggatc caccgagcag
     4801 atcgcagtgc acgatcgcga gcgatgatct cccggcccgc aggtgcacga cccgggcgcg
     4861 gaggcgggac cggaatccct cgccgtcgtg ggcgttggag ctgtaaccgg ccttgggcat
     4921 gccgggcggc ggggtgatgt cgacgctcgc ggccccggcg agcaccccgt cgacgtggtc
     4981 gaggcggacc ggtgccgccg gtttcacctc gaacgacgcc cggcccgacc gcagatgcgc
     5041 cacggagtcg agggcagacc cgctcacgat ttagccgccg atttagccgc cgatttggcc
     5101 gccgatttgg ccgccgagtt catgcggcgg aagaacgact tctgtcgctt gtagaggccc
     5161 ttgaactcct tgtagtggcg gtcgtagatc gccgtatgga gtgcgttggg gcgataggtc
     5221 gccttgatcg acaccaggtc gcgcacctcg tcgggacgaa cctcgcccat cgccatcgcc
     5281 gcgctcagcg ctgcgcctcg gataccgcaa tagagcgggt cggcaac
//
LOCUS       DAOOMA010000106         5298 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_18701_length_5298_cov_3.776464, whole genome shotgun sequence.
ACCESSION   DAOOMA010000106 DAOOMA010000000
VERSION     DAOOMA010000106.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 5298)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5298)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5298
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18701_length_5298_cov_3.776464"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..189
                     /locus_tag="PLP95_04265"
     CDS             <1..189
                     /locus_tag="PLP95_04265"
                     /inference="COORDINATES: protein motif:HMM:NF012797.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrmH family RNA methyltransferase"
                     /protein_id="HPB45048.1"
                     /translation="APLDGAMAIVLGNEAHGLDEAAIAACDSAVTITMDGATESLNVA
                     MAGTVLAFESMRRRLTAT"
     gene            complement(295..1209)
                     /locus_tag="PLP95_04270"
     CDS             complement(295..1209)
                     /locus_tag="PLP95_04270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB45049.1"
                     /translation="MGAWNRTDDASPDRANGRLRRWRYRLTRAREQFLLSWADYSLAQ
                     RVKGIGLSGAVAAIAIVVIVGGRGPSGAIIIAQDSARSESSAEASPEETAPPATVQLD
                     GETLAVEEIDATALSDEELIEILHQMNAAALAEVMGATEERTDSGTETTTRDGNTTTA
                     KKAKQPSRADLEALFAAMQREAAAATAPAPAPTASATRAPATTRPPRATTTDEPTTTK
                     PPKTTTTDETTTTKPPKTTTTEAPTTTKPPKTTTTEAPTTTRPPRTTTEAPTTQPPTT
                     EPPTTQRPTTQPPTTEPPEGPGEPGPED"
     gene            1363..1755
                     /locus_tag="PLP95_04275"
     CDS             1363..1755
                     /locus_tag="PLP95_04275"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005065009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HPB45050.1"
                     /translation="MTVVRINAIEVPEGLGATLEERFAARLHAVDQAPGFRGFEMLRP
                     TGEAEKRYFIVTHWATAEDFDNWVNSADFAKGHGHADGAGGAAGGHPGGHPGGHPGGA
                     VGGHPGAGPVATGSAVLEFEVVLESKPT"
     gene            complement(1833..3008)
                     /locus_tag="PLP95_04280"
     CDS             complement(1833..3008)
                     /locus_tag="PLP95_04280"
                     /inference="COORDINATES: protein motif:HMM:NF013253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HPB45051.1"
                     /translation="MANHGDWQLGIYFDGLEGVTPEFPMTFDELEAAAHEALAPHLWS
                     YVSGGAGNEYTQRANVSAFERWGLIPRILAGAAQRDLGVEMFGVAYDTPLMLAPVGVI
                     GICEQSGHGDITTAHAAAATNTPMIASTLMQDPMEEVAAALGDTPGWFQLYPPSDRDV
                     CESLIRRAEAAGYQAIVVTVDTLTLGWRPRDLTIASFPQLNGLCLANYFSDPVFRSKL
                     AVPPEQDPTAATGAWAFGFGNPGMDWNDLEWIRSVTDLPLLVKGVNHPDDARKALDHG
                     VNGIYCSNHGGRQANGGIPAIDLLPDVVAACGDAPVVFDSGIRSGTDMAKALALGATA
                     VAIGRPYTYGLAIGGQAGVEHVIRSLLAELDLLMAVDGYASVSDLTPDAMRRVSPPA"
     gene            3139..4176
                     /gene="pheS"
                     /locus_tag="PLP95_04285"
     CDS             3139..4176
                     /gene="pheS"
                     /locus_tag="PLP95_04285"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012226834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HPB45052.1"
                     /translation="MSDSPSGSSSGGDLLAQIASIVEAAQQAIGVADSLDALRHADSD
                     HLGKASKLSALKSQMGTLDHEGKRSVGKALNEARTTLEALVAQRRDAFEAAERASRLE
                     AERLDLSEAIVDASVGHMHLISQTLERLEDVFCGLGFTVAEGPEVETDWYNFEALNMP
                     EGHPARSMWDTLYLEAGEPETILLRTHTSPVQVRTMMRQEPPIYIVAPGRAFRSDTAD
                     ATHLPVFHQIEGLVVDRGITFADLQGTIEAFVKALYGPDRRTRLRPSYFPFTEPSAEF
                     DMTTASGEWVELGGCGMVNPNVLRSCGLDPEEWSGFAFGFGIERMAMQRHGMDDIREF
                     VTNDVRFLAQF"
     gene            4283..>5298
                     /locus_tag="PLP95_04290"
     CDS             4283..>5298
                     /locus_tag="PLP95_04290"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:NF015448.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HPB45053.1"
                     /translation="MKVLHNWLAEYVPGLLDHPIGGDAEALGERMTELGLCCEEVVRL
                     GNLEGIVVGEVLERSPHPNADRIQLVRVDIGAGGEPLHICCGATNFEVGDKVPLATLG
                     TTMPGGMVIEKRKLRGELSEGMMCSGRELEIGDDHSGILILDESAAIGAPIAGVLGIE
                     ADALFDLDLTPNRPDAMSVIGIARDVAAGLGLEFVLPEIDVAESGSPAADVASVSISD
                     PDLCGRFTARVLTGVKIGTSPEWMQQRLIHLGMRPINSVVDISNYVMLEYGQPNHTYD
                     LAKVSGGALGVRRAVEGETIVTLDGQTRTLVSRDGVIVDGADVAVGIAGVMGGADTEI
                     GDST"
BASE COUNT          804 a         1683 c         1851 g          960 t
ORIGIN      
        1 gctccgctgg atggcgcgat ggcgatcgtg ttgggtaacg aggcacacgg tctcgacgag
       61 gcagcgatcg ccgcgtgcga ttcggcggtc accatcacca tggacggagc gaccgaatcg
      121 ctcaacgtcg cgatggccgg cacggtgttg gccttcgagt cgatgcgcag gcgcctgacc
      181 gcgacctgac gcaaggtcgt gcctgacgcg ttgcggcttg ggggctgagg tcggcggcca
      241 gcgggttcag cggccagcgg gttcagcggc cagcgggttc ggcagctggc aggttcagtc
      301 ctccgggccc ggctcgccag gtccctccgg cggttcggtc gtcggaggtt gagtggttgg
      361 gcgctgggtg gttgggggct cggtcgtcgg cggttgggtg gttggggcct cggtggtggt
      421 ccgcggcggc cttgtggtgg tgggagcctc agtcgtcgtg gtcttgggcg gcttggtggt
      481 cgtgggcgcc tcggtcgtgg tggtcttggg cggcttggtg gtggtcgtct cgtcggtcgt
      541 ggtggtcttg ggcggcttgg tggtggtggg ctcgtcggtc gtcgtggctc tcggcggcct
      601 ggtcgtggca ggggcgcgtg tggccgacgc cgtgggagcc ggggccggcg ccgttgcggc
      661 agccgcctcc cgttgcatcg cggcgaacaa cgcttcgagg tcggctcggc tcggctgctt
      721 ggccttcttg gcggtggtgg tgttcccgtc gcgggtggtc gtttcggtgc ccgagtcggt
      781 tcgctcctcg gtcgctccca tgacctcggc gagggccgcg gcgttcatct ggtgcaggat
      841 ctcaatcagt tcttcatcgc tcaatgccgt cgcatcgatt tcctccaccg cgagggtctc
      901 cccgtcgagt tgcaccgtcg ccgggggagc ggtttcttcg gggctggcct cagcgctgct
      961 ctcggagcgg gcggagtcct gggcgatgat gatcgcacct gaagggcctc gtccaccgac
     1021 gatgacaacg atggcgatgg cggcgacggc ccccgagagc ccgatgccct tgacccgttg
     1081 ggcgagcgaa tagtcggccc atgacaggag gaattgctcg cgggcgcggg tcaggcgata
     1141 gcgccacctc cgcaatcgcc cgttcgcccg atcgggcgat gcgtcgtcag ttcgattcca
     1201 cgcacccatg cctcagaagc tcctcaaaac ccggctcgac gatcttcagc agtttagaca
     1261 gagtttcaac tcatacaagg gcatcctcgg cgctctgatg cccctcagtg tcggccgtca
     1321 cagtcggccg tgcgcggcgt cgctgcgtcg taggctcgcc ccatgactgt cgtacgaatc
     1381 aatgccatcg aggttcccga gggattggga gccacactcg aggagcgttt cgccgctcgc
     1441 ctgcacgctg tggaccaggc gcccgggttc cgcgggttcg aaatgctgcg gcccacgggc
     1501 gaagcggaga agcgctactt catcgtgacg cactgggcga cggccgagga cttcgacaac
     1561 tgggtgaact cggcggattt cgcaaagggt cacggccatg ctgacggcgc gggtggcgct
     1621 gctggaggtc atcctggtgg tcatcctggt ggtcatcctg gtggtgctgt tggaggtcat
     1681 ccgggcgcag ggccggttgc cacggggtcg gctgttctcg agttcgaggt cgtgctcgag
     1741 tcgaagccga cctgacccgc ggcgcgctga cccgcggcgc gctggcgcgg gcagctccca
     1801 cggcgggttg ctgcgatgcg gtgtcggaac cgtcaggcgg gtgggctgac gcggcgcatg
     1861 gcgtcgggcg tgagatccga caccgacgcg tagccgtcga ccgccatgag gaggtcgagt
     1921 tcggcgagca ggctgcggat gacgtgttcg acaccggcct gtccgccgat cgccaacccg
     1981 taggtgtagg ggcggccgat ggcgacggcg gtcgcgccga gggccagcgc cttggccatg
     2041 tcggtacccg agcggatacc cgaatcgaac acgaccggcg catccccgca cgcggccacg
     2101 acgtcgggta acaggtcgat cgccgggatt ccgccgttgg cttgtcgtcc gccgtggttc
     2161 gaacagtaga tgccgttcac cccatgatcg agcgccttgc gggcgtcgtc gggatgattc
     2221 acccccttca cgagcagcgg tagatcggtg accgaacgga tccactccag gtcattccag
     2281 tccatgccgg ggttgccgaa tccgaacgcc cacgcgccgg tcgctgcggt tgggtcctgc
     2341 tcgggcggca ccgcaagttt ggaccgaaac accgggtcgc tgaagtagtt ggcgaggcac
     2401 aaaccgttga gctgcgggaa gctcgcgatg gtgaggtcgc gggggcgcca gccgagggtg
     2461 agcgtgtcga cggtgacgac gatggcctgg taaccggcgg cctcggctcg gcggatcagg
     2521 ctctcacaga cgtctcgatc cgagggaggg tagagctgga accaccccgg agtatcgccg
     2581 agcgcggcgg ccacctcctc catcgggtcc tgcatgagcg tcgacgcgat catcggggtg
     2641 ttcgtggcgg cggcagcgtg cgccgtcgtg atgtcgccgt ggccgctttg ttcgcagatc
     2701 ccgatcaccc ccaccggggc gagcatcagc ggagtgtcgt aggcgacccc gaacatctcg
     2761 acgccgaggt cgcgttgcgc ggccccggcg aggattcgcg ggatcagccc ccagcgctcg
     2821 aacgcgctga cgttggcccg ttgggtgtat tcgtttcccg caccgcccga cacataggac
     2881 caaagatgtg gtgccagtgc ctcgtgcgcg gcggcctcga gttcgtcgaa ggtcatgggg
     2941 aactcggggg tcaccccctc aagtccgtcg aagtagatgc cgagttgcca atctccgtga
     3001 ttcgccacgt gcgccccctt gaagccgttc cccatgatgg ccgatgtcga cggggcgggg
     3061 gagtgtcgac gggtttgcgc cgtcggcctc gccgcaatgg gttcgcggcg tcggcggctg
     3121 agcgggcaga atggacggat gtccgattct ccatccggtt cctcgtccgg cggtgacctc
     3181 ctcgcccaga tcgcgtcgat cgtcgaggcg gcgcagcagg ctatcggcgt cgccgactct
     3241 ctcgatgcgc ttcgccacgc cgacagcgac cacctcggca aagcgtcgaa gctgtcggca
     3301 ctgaagtctc aaatgggcac gctcgatcac gagggcaagc ggtcggtcgg caaagcgctc
     3361 aacgaggccc gcacgacact cgaggcattg gtggcgcagc gtcgagacgc attcgaggcc
     3421 gccgagcgag cctcgcgtct cgaggccgaa cgcctcgatc tgtccgaggc aatcgtcgac
     3481 gcgtcggtcg gccacatgca cctgatctcc cagaccctcg aacggcttga ggacgtgttc
     3541 tgcgggttgg ggttcaccgt cgccgaaggg cccgaggtcg agaccgactg gtacaacttc
     3601 gaggcgctca acatgcccga ggggcacccg gcccgctcca tgtgggacac gctgtatctc
     3661 gaggcgggag agcccgagac gatcctgttg cgcacgcaca ccagccccgt gcaggtccga
     3721 acgatgatgc gccaggagcc tcccatctac atcgtggccc cgggccgggc gtttcgttcc
     3781 gataccgccg atgccaccca cctgccggtg tttcaccaga tcgaagggct cgtcgtcgac
     3841 cgtggtatta cgttcgccga cctgcaagga acgatcgagg cgttcgtgaa ggcgttgtac
     3901 ggccccgatc gacgcacccg ccttcggccg tcgtatttcc cgttcaccga gccgtctgcc
     3961 gagttcgaca tgacgaccgc atcgggggag tgggtcgaac tcggcggctg cggcatggtc
     4021 aaccccaacg tgttgcgttc ctgtgggctc gaccccgagg agtggtcggg gttcgcgttc
     4081 gggttcggga tcgaacggat ggcgatgcaa cgccacggca tggacgacat ccgcgagttc
     4141 gtcaccaacg acgtccgatt cctggcgcag ttctagccgc gcagacccaa ccgcgcagtt
     4201 ccaaccgcac agttctcacc accaccgcct accaccatcg ctcatcacca ccgcttagaa
     4261 ggcacagctc agaaagcaca cgatgaaggt tcttcacaac tggctcgccg agtacgttcc
     4321 aggactcctc gatcatccga ttggcggcga cgccgaggcg ctcggcgaac gcatgaccga
     4381 gctcggtctc tgctgcgagg aggtcgtgcg actcggcaat ctcgagggaa tcgtcgtcgg
     4441 cgaggtcctc gagcgctcgc cgcacccgaa cgccgatcgc atccaactcg tccgcgtcga
     4501 catcggtgct ggcggcgagc cgctgcacat ctgctgcggc gccacgaatt tcgaggtcgg
     4561 agacaaggtg ccgctcgcaa cgctcggcac caccatgccc ggcgggatgg tcatcgagaa
     4621 gcgcaaactt cgcggtgaac tctccgaggg catgatgtgt tcgggccgcg agttggagat
     4681 cggcgacgac cactccggaa tcctgatcct cgatgaatcg gcggcgatcg gcgctccgat
     4741 cgccggcgta ctcggcatcg aagccgacgc gctgttcgac ctcgacctca ctccgaatcg
     4801 ccccgacgcc atgtcggtga tcggtatcgc ccgcgatgtc gctgccggcc tcggcctgga
     4861 gtttgtgctt ccggaaatcg atgttgccga atcgggttcg ccggcggccg acgtcgcgtc
     4921 ggtgtcgatc tcggatccgg acctttgcgg tcgattcacc gctcgggtgc ttacaggcgt
     4981 caagatcggc acctcgccgg agtggatgca acagcgactc atccacctcg ggatgcgccc
     5041 gatcaacagc gtcgtggata tctcgaacta cgtgatgctc gagtacggac agccgaacca
     5101 cacctacgac ctcgccaagg tgtccggcgg tgcgctgggc gtgcgccggg ccgtggaagg
     5161 ggaaacgatc gtcaccctcg acgggcagac ccgaaccttg gtctctcgag acggggtgat
     5221 cgtcgatggt gcggatgttg cggtcggaat cgccggcgtg atgggtggtg ccgacaccga
     5281 gatcggcgac agcaccga
//
LOCUS       DAOOMA010000107         5284 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_18749_length_5284_cov_4.059667, whole genome shotgun sequence.
ACCESSION   DAOOMA010000107 DAOOMA010000000
VERSION     DAOOMA010000107.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 5284)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5284)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5284
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18749_length_5284_cov_4.059667"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..703)
                     /locus_tag="PLP95_04295"
     CDS             complement(<1..703)
                     /locus_tag="PLP95_04295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012226367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HPB45054.1"
                     /translation="METRAAVLWNVGEEWKIENITLDDPGPGDVLVEMKVAGMCHSDE
                     HVVTGDMLMPHYPCIGGHEGAGVVLAVGSHVTSVEVGDHVSMSFIPSCGRCKYCASGR
                     QNLCNEGAKLFDLGMMSDGRVAHHIAGTDTPASRMCQVGTFAEHVLVSENSVVKVDKD
                     LPWHAVALVSCGVATGYGSAVHRAGVKPGDNVAVVGCGGIGMNAVQGAKLAGAKRVIA
                     IDPVEFKREQAMEFGA"
     gene            958..1599
                     /locus_tag="PLP95_04300"
     CDS             958..1599
                     /locus_tag="PLP95_04300"
                     /inference="COORDINATES: protein motif:HMM:NF015513.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HPB45055.1"
                     /translation="MPTPGRPGRGARWFDPDDPGDPRRFGRRPPGPKGGGSFGGGPRG
                     GGPGGRGRARRGQIRTALLALLAEAPMHGYEMIRTLDERSGGRWRPSPGAVYPTLSQL
                     EDEGLIEANESDGKRVFRLTEPGREAAESLAAQDREPWAGDDVAAGFELRGLVAQLGH
                     AVRGVAMGADASQQAKVAEVLVEARRSIYRILADEPTADSGDEGGDDPDGIDA"
     gene            complement(1651..2199)
                     /gene="sufT"
                     /locus_tag="PLP95_04305"
     CDS             complement(1651..2199)
                     /gene="sufT"
                     /locus_tag="PLP95_04305"
                     /inference="COORDINATES: protein motif:HMM:TIGR03406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative Fe-S cluster assembly protein SufT"
                     /protein_id="HPB45056.1"
                     /translation="MSAAWNPIHVSRDTRASTVPAGNPVVLAEGTEVTIIQQLGGSVT
                     VRNQMGALLRIDGADADALGIEIEDIGLRARSDGPFEMSQVLEALGEVYDPEIPVNIV
                     ELGLVYRCEEHQRADGGRRIEIDMSMTAPGCGMGDVLRLDAERAVAAVPGVDEVVVEV
                     VWFPPWNMGLMSDAARLQLGLM"
     gene            complement(2196..3473)
                     /gene="sufD"
                     /locus_tag="PLP95_04310"
     CDS             complement(2196..3473)
                     /gene="sufD"
                     /locus_tag="PLP95_04310"
                     /inference="COORDINATES: protein motif:HMM:TIGR01981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HPB45057.1"
                     /translation="MTTPASLLDRLAPQVNTGVRAERGRTWFRAHGFPNPRQEAWRYT
                     PVDEIARVIAEGTPGPHATAGIDAATIDRLAGSHGGPRMVFLNGALARELSDLDSEIQ
                     GLVLAGVDDLRPRRRPDQLPPTDEPADGFHALNWAAGRDVAGIIVESDTTPDVPVHVV
                     HLAVPGESVSVSHPRTVIAIRPRSRATVIESFVGLGGRAVTNSSTRIVVGDDATLTYH
                     RLMAEPEGTIHVARTAMMQSRGSTVTGRSIICGGDIVRNAIEVTLSGDGATSNLEGLY
                     LPIGSERHDNMITVDHAASHGRSMQRFKGIVDDHGRGSFSGHVIVSHGTVGNDADQSN
                     PNLVLTPAAQADSRPWLEIYADDVACNHGATVGRLDSDALFYLRSRGIPATEARSMLV
                     AAFAAEILDAVEPTSLREWLDAVIARRHGSRTA"
     gene            complement(3470..4219)
                     /gene="sufC"
                     /locus_tag="PLP95_04315"
     CDS             complement(3470..4219)
                     /gene="sufC"
                     /locus_tag="PLP95_04315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HPB45058.1"
                     /translation="MLSIHQLTASVDGRPILKGLDLEIGPGEVHAIMGPNGAGKSTLS
                     NVLAGRDGYEVGGTATLDGIDLLTIDPEARAAAGLFLSFQYPVEIPGVGNMYFLRTAL
                     NALRRSRGVSEVSAVDFLSLARQHMAQLEMDPAFLSRGVNEGFSGGEKKRNELLQMSL
                     LEPRLAILDEIDSGLDIDALRIVAAGVERLRNPQRSMLIITHYPRLLEAVRPDRVHVL
                     ADGRITHSGGYELALELEEHGYSLTAESGAA"
     gene            complement(4219..>5284)
                     /gene="sufB"
                     /locus_tag="PLP95_04320"
     CDS             complement(4219..>5284)
                     /gene="sufB"
                     /locus_tag="PLP95_04320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012224420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HPB45059.1"
                     /translation="VDAIIDSVSVATTFKAKLAEVGVIFCSFSDAVQEHPELVRKYLG
                     TVVAPRDNYFAALNSAVFSDGSFAYIPAGVACPMELSTYFRINAKETGQFERTLIVAE
                     EGASVSYLEGCTAPMRDENQLHAAVVELVALDDSSIKYSTVQNWYPGDAEGRGGIYNF
                     VTKRGRAANRAKISWTQVETGSAITWKYPSVILQGDDSVGEFYSVAVTNNCQQADTGT
                     KMIHIGRNTSSTIVSKGISAGRAQNTYRGQVKVLRSATGARNYTRCDSLLIGGDCGAH
                     TFPYIEVKNDSAEIEHEASTSKISDDQLFYCRQRGLTEEDATSMIVNGFCREVFDELP
                     MEYAVEAQALMRVSLEGAVG"
BASE COUNT          879 a         1619 c         1797 g          989 t
ORIGIN      
        1 tggcgccgaa ctccatcgcc tgttcgcgct tgaactcgac cgggtcgatg gcgatgacgc
       61 gtttggctcc ggcgagcttt gctccctgaa ctgcgttcat tccgatacca ccgcaaccga
      121 ccaccgccac gttgtcgccg ggcttgactc cggcgcgatg taccgccgag ccgtatccgg
      181 tggcgacccc gcagctgacc agcgccactg cgtgccatgg tagatccttg tcgaccttga
      241 cgacggagtt ttcgctgacc agcacgtgtt cggcaaatgt gccgacctga cacatgcgcg
      301 acgcgggggt gtcggtgccg gcgatgtggt gggcgacgcg tccgtcactc atcatcccga
      361 ggtcgaacag cttggcgccc tcgttgcaga ggttctggcg tccggatgcg cagtacttgc
      421 agcgtccgca gctcgggatg aacgacatcg acacgtgatc gcccacttcg acgctggtga
      481 catgcgagcc gacggcgagc acgaccccgg cgccttcgtg gccaccgatg cacgggtagt
      541 gcggcatcaa catgtcaccg gtgacgacat gttcgtccga gtggcacatg ccggcgacct
      601 tcatctcgac gaggacatcg ccgggtccgg ggtcgtcgag ggtgatgttt tcgatcttcc
      661 actcttcgcc cacgttccac aggactgctg cgcgtgtttc catcggtttc ccccttgttg
      721 gatcgaccgc tcggcccctg cacgaacgtc gaacgttgtt gtctctttct acacccccga
      781 tggtgggtcg gggtggcgag gaggtgtgag ggaacagata gttggtatat gacgatatat
      841 cgtgatatag attactccgg tcgatggaac gtccacgacc gatgaaagga gtacgtcatg
      901 aacacacgac gtgagaatga agaaacttgt gcaccggcga tgttcgtctt cggtgcaatg
      961 ccaactccgg gccggcctgg ccgcggtgct cgttggtttg accccgacga ccctggagac
     1021 ccgcgacggt tcggtcggcg accgcctggt ccgaagggtg gtgggtcatt cggcggcggc
     1081 ccgcgaggcg gcggtccggg tgggcgagga agggcgcgcc gagggcagat tcgcaccgcg
     1141 ctgctcgcgt tgctggccga agcgccaatg cacggctacg agatgatccg taccctcgac
     1201 gaacgcagcg gcgggcggtg gcggccgagt ccgggcgctg tgtatccgac cctgagccaa
     1261 cttgaggacg aggggttgat cgaggcgaac gagtccgacg gcaagcgcgt ctttcggttg
     1321 accgaaccgg gtcgcgaggc cgccgaatcg ctcgctgcgc aagaccgcga accgtgggcc
     1381 ggcgacgacg tcgccgccgg tttcgaactc cgagggctcg tggctcaact cggccacgcg
     1441 gttcgagggg tggcaatggg tgccgatgcc tcgcagcagg ccaaggtggc cgaggtgctc
     1501 gtggaggcaa ggcggtcgat ctatcgaatc ctcgccgatg aacccacggc ggattccggt
     1561 gatgagggcg gcgacgaccc cgacgggatc gacgcgtagg tgacgacgtc ggcgcggcga
     1621 gcggggtact tcggtgttgt gccccgctcg ctacatcaag ccgagttgga gccgggcggc
     1681 gtctgacatc aggcccatgt tccacggtgg gaaccacacc acctcgacca ccacctcgtc
     1741 gacgcccggc accgcggcca cggcccgttc agcatcgagg cgtaacacat cgcccatccc
     1801 gcaacccggg gcagtcatcg acatgtcgat ctcgattcga cgtccgccat cggctcgttg
     1861 gtgttcctca catcgataga cgaggccaag ttcgacgatg ttgaccggga tttcggggtc
     1921 gtaaacctcg ccgagggcct cgagcacctg actcatctcg aaggggccgt cgctccgggc
     1981 gcggaggccg atgtcttcga tctcgatacc gagcgcatcg gcatcggcgc cgtcgatgcg
     2041 caacagggca cccatctggt ttcgaaccgt gaccgagcca ccgagttgtt ggatgatcgt
     2101 gacctcggtg ccctcagcaa ggaccacggg gttgccggcg ggaaccgtcg aagcgcgggt
     2161 gtctctggag acatggatgg ggttccacgc cgcgctcatg cggtcctgct tccgtgacgt
     2221 cgtgctatca cggcatcgag ccattctcgc agcgatgtcg gctcgaccgc gtcgaggatc
     2281 tcggcagcga acgccgccac gagcatcgag cgggcctcgg tcgccggaat gccgcggctg
     2341 cgcagataga acaacgcgtc ggagtcgaga cgaccgaccg ttgccccgtg attgcaggca
     2401 acgtcgtcgg cgtagatctc gagccatggt cggctgtcgg cctgagcggc cggggtgagc
     2461 accaggttcg gatttgattg gtcggcatca ttgccgaccg tgccgtggct cacgatgaca
     2521 tggccgctga acgagccgcg accgtgatcg tccacgatgc ccttgaaccg ttgcatcgac
     2581 cggccgtggg aggccgcatg atcgacggtg atcatgttgt cgtgacgctc tgatccgatc
     2641 gggagataca gaccctcgag attcgaggtt gcgccgtctc cgctcagggt gacctcgatg
     2701 gcatttcgca cgatgtcgcc gccacagatg atgctgcgcc cggtgaccgt cgatccgcgg
     2761 gactgcatca tggcggtgcg tgcgacgtgg atggtgccct cgggttcggc catcagccgg
     2821 tggtaggtca gggtggcgtc gtcgccaacc acgattcggg tcgacgagtt cgtcaccgcc
     2881 cgacccccga gacccacgaa ggactcgatg accgtggcgc ggctgcgggg ccggatcgcg
     2941 atgacggttc gtgggtgcga gaccgaaacc gattcgcctg ggactgcgag gtgaacgacg
     3001 tgaacgggta catcgggggt cgtatcggac tcgacgatga ttcctgcgac gtcgcgtcct
     3061 gccgcccagt tgagggcatg gaagccgtcc gcaggttcat cggtaggggg caactgatcg
     3121 ggtcggcgtc gagggcgcag gtcatcgacc ccggcgagga ccagcccctg aatctcggaa
     3181 tcgagatcgg acagttcgcg agcgagcgct ccgttgagga acaccatgcg tggtccgccg
     3241 tggcttccgg ccaggcggtc gatggtcgca gcatcgattc cggcagtggc gtgaggacca
     3301 ggagttccct cggcgatcac ccgggcgatt tcatcgaccg gcgtgtagcg ccacgcctct
     3361 tggcgtgggt ttggaaaccc gtgagcgcga aaccaggtcc tgccgcgctc ggctcggaca
     3421 cccgtgttga cctgcggtgc gagtcggtcg agcagtgagg ccggggtggt catgcggcgc
     3481 cactttcggc ggtcagggag tagccgtgct cctcgagttc gagcgcgagt tcatagccgc
     3541 ccgagtgggt gatgcgcccg tcggccagca cgtggacccg gtcgggtcgc accgcttcca
     3601 acagacgcgg gtagtgggtg atgatcagca tcgaccgttg cggattccgg aggcgttcga
     3661 cccccgccgc aacgatgcga agggcgtcga tgtcgaggcc tgagtcgatc tcgtcgagga
     3721 tggccaatcg gggttcgagc agcgacatct gcaggagttc attgcgcttc ttctccccgc
     3781 cggagaaccc ctcgttgacc cctcggctga ggaaggccgg atccatctcc agctgggcca
     3841 tgtgctgtct ggcgaggctg aggaagtcga cagcggagac ctcggagact ccccggcttc
     3901 ggcgtagcgc attcagcgcg gtacgcagga agtacatgtt gccgactccg gggatttcca
     3961 cggggtattg aaaggacaga aacaacccgg cggcagcgcg tgcctcgggg tcaatggtca
     4021 acaggtcgat cccgtcgagg gtggcggtgc cgccgacctc gtagccatcg cggccggcga
     4081 gcacgttgga cagggtggac ttcccggccc cgttcgggcc catgatcgca tggacctctc
     4141 cggggccgat ctcgaggtcg agacccttga ggatcggtcg gccgtcgacc gatgcggtga
     4201 gttggtggat ggacagcatc accccaccgc cccttcgagg ctcacccgca tgagcgcctg
     4261 tgcttcgacg gcgtattcca tggggagttc atcgaacacc tcgcggcaga atccgttcac
     4321 gatcatggat gtcgcatcct cctcggtcag cccccgttgt cggcaataaa agagctggtc
     4381 gtcgctgatc ttcgaggtgg aggcctcgtg ttcgatctcg gcgctgtcgt tcttcacctc
     4441 gatgtagggg aaggtgtgcg caccacagtc gcctccgatg aggagcgagt cgcagcgggt
     4501 gtagttgcgg gcgccggtgg ccgagcgcag caccttgacc tgcccgcggt aggtgttctg
     4561 tgccctgcca gccgagatgc ccttggaaac gatcgtgctc gacgtgttgc ggccgatgtg
     4621 gatcatcttg gttccggtgt cggcctgctg gcagttgttg gtgaccgcca ccgagtagaa
     4681 ctcgccgacg gagtcgtctc cttggaggat cacgctcggg tatttccacg tgatggcgga
     4741 tccggtctcg acctgggtcc aggagatctt ggcccggttc gcggcgcgcc ctcgcttggt
     4801 gacgaagttg tagatcccgc cccttccttc ggcatcgccg gggtaccagt tctgcacggt
     4861 cgagtacttg atggaggagt cgtcgagcgc gaccagttca accacggcgg cgtggagttg
     4921 gttctcgtcg cgcatcggcg cggtgcaccc ctcgaggtag ctgacgctgg ctccctcttc
     4981 cgcaacgatg agtgtccgtt cgaactggcc ggtctccttg gcgttgatcc ggaagtaggt
     5041 cgacagttcc atcgggcagg caacccccgc agggatgtag gcgaacgagc cgtcggagaa
     5101 caccgccgag ttcagtgcgg cgaagtagtt gtcgcgcggt gcaacgaccg ttccgaggta
     5161 cttgcgaacg agctcggggt gttcctgaac cgcgtcggaa aaggagcaga agatgacgcc
     5221 gacctcggcg agtttcgcct tgaaggtggt ggccaccgat accgaatcga tgatcgcgtc
     5281 cacc
//
LOCUS       DAOOMA010000108         5263 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_18840_length_5263_cov_4.449885, whole genome shotgun sequence.
ACCESSION   DAOOMA010000108 DAOOMA010000000
VERSION     DAOOMA010000108.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 5263)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5263)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5263
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18840_length_5263_cov_4.449885"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..490)
                     /locus_tag="PLP95_04325"
     CDS             complement(<1..490)
                     /locus_tag="PLP95_04325"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: protein motif:HMM:NF013421.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HPB45060.1"
                     /translation="MNSSDGNVVASTTTSATASAPSHRGLRTYLPMIAIALGVLVIDQ
                     ITKAWANGDWIFSGLDRCNSLVERPALGFCLAFNEGMAFSIGWGSGALISIVVVSVIV
                     TLAVFSRNVSLAQRLVMGAIVGGALGNLIDRGLRPAAPGTLDRGFMGGAVVDFIYSRY
                     WAT"
     gene            complement(500..1315)
                     /locus_tag="PLP95_04330"
     CDS             complement(500..1315)
                     /locus_tag="PLP95_04330"
                     /inference="COORDINATES: protein motif:HMM:NF013427.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA C4-type zinc finger protein"
                     /protein_id="HPB45061.1"
                     /translation="MPAKKATTSKAATSAKKAAPAKKAPAKAPAAKKAPAKKAPAKAP
                     AAKKAPAKKAPAKAATKSPAKAKAPAKAPAKKAATAKAPAKAPAKKAATAKAPAKKAP
                     AKTAAKTAATAKAPAKTAPPVSPVRSVLGSLPRPGANPEPRLTTSPYDAKFLESQKAM
                     LLDERATYSRQAEVLFEEAQSLTANREPGDVQFDEESGEGDTLAVARDQDLELAARAR
                     STVEEIDAALERIANKTYGICAASGLAIPEERLKAIPWATERVEYKVGGFGRL"
     gene            complement(1480..2919)
                     /locus_tag="PLP95_04335"
     CDS             complement(1480..2919)
                     /locus_tag="PLP95_04335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HPB45062.1"
                     /translation="MDQTPQRLPYDFNEKFRGYDIDEVDAYLLQLSGQLDALGLERDD
                     ALARAGSAEQEIVRLREALSAAQAAAAVPAQAVVAPSPSSVSDEATAAEAARTLMLAS
                     QTAERAVAEAKSDAAKLVADATTNADRITRDARVEADRVVSAARASADQMTQEASEQA
                     AREYGARRDEVLAEIATLDSERASSVTQLAAIETRVEEYRSALSGLVDHITAALDSPE
                     RMLRPPLPGIELPAPAQFRDTATDTAVDTGTGGASSPQSPTFLASAETGSSVSDPVVA
                     AEPGEAQPAPAIADTFDSAESAEAESGAYGTDAGAPVPLQYLDPEAESASVDPGSFVV
                     ETDPVDFDTPSPLPAAEAFGEDSPAASPVDHAGFDTGFDEGWESPSEPQSQGASDPWA
                     PGSWREVSGLSMTSDGFTPEPQPDYGVDAFAGDEPVAAVGLPEIGEDRYAQDLHEALA
                     VSDGGEDEAMERFFGGDDERQTRRFGRRR"
     gene            complement(3007..3660)
                     /locus_tag="PLP95_04340"
     CDS             complement(3007..3660)
                     /locus_tag="PLP95_04340"
                     /inference="COORDINATES: protein motif:HMM:NF016363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HPB45063.1"
                     /translation="MSGMLRKGMSWLGLGPDDGYDGYDDYDDYDDDDVYYDEPQVAPA
                     PSNPVVRAVPNPAQRGAGPRSGGARGPSQRAASSRVEPDDDWGDSAEPSAVRVLPTAA
                     RTTTSAEPVRPRSSAVRPIPATPASARPHTVTPSSFNDAQEVGDHFKKNQPVIVILNE
                     LDRGLARRMLDFSSGVAYGLGGSVERLAASVYLLTPADVELSSEERRRLRERGILDD"
     gene            complement(3737..4483)
                     /locus_tag="PLP95_04345"
     CDS             complement(3737..4483)
                     /locus_tag="PLP95_04345"
                     /inference="COORDINATES: protein motif:HMM:NF014619.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphenol oxidase family protein"
                     /protein_id="HPB45064.1"
                     /translation="MALRATFESPGRQPVSLAFSEAADGDFAIDSEAGVLGRSRQRLM
                     AGPWTALRQVHGDRVMIVDAPGDHLGAEGDGLVTSVPGVVVAVQSADCVPVLFASVDG
                     AEVVAAAHAGWRGLYEGIIERTVESMRELGAGAIRAWIGPCISAPHYEFSEADLTTMA
                     FRFGPEVIAATVASRPALDVVAAARVALAGVGVDEISDEFAWCTATSTVNGEPQFASH
                     RSRAEARRQAAAIRLGVPEATPAPGDHDTA"
     gene            complement(4487..>5263)
                     /locus_tag="PLP95_04350"
     CDS             complement(4487..>5263)
                     /locus_tag="PLP95_04350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012224643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HPB45065.1"
                     /translation="APVVANIAKETVGALTIAVVTRPFLFEGRRRSVQAEQGITKLKE
                     NVDTQIVIPNDRLLSVANDKTSVLDAFRMADEVLLQGVQGITDLITTPGLINTDFADV
                     KMIMSAAGTALLGVGYGSGEGRATAAARQAISSPLLESEIDGARGILLNICGGSALTL
                     HEVNEAATVVHEVAHAEANIIFGTTIDDDMGDEVRVTVIGAGFERWEDPKAAVPTRRA
                     PSNLSERRSRSVESEPPIADVFGDDDDDDETEFDIPSFLK"
BASE COUNT          846 a         1826 c         1728 g          863 t
ORIGIN      
        1 aggtcgccca gtagcggctg tagatgaagt ccaccactgc gccgcccatg aaaccgcggt
       61 cgagcgttcc gggcgcagcc gggcgcaatc cccggtcgat gaggttgccg agggctcctc
      121 cgacgatcgc tcccatgacg aggcgctgcg cgagcgagac attgcgcgag aacaccgcga
      181 gggtcacgat caccgacacg accacgatcg agatgagcgc gccggacccc cagccgatgc
      241 tgaacgccat cccctcgttg aacgccaggc aaaagccgag cgccggacgc tcgaccaacg
      301 aattgcagcg atcgagaccg ctgaagatcc agtcgccgtt cgcccaggcc ttggtgatct
      361 gatcgatcac caaaacgccg agcgctatgg cgatcatcgg caggtaggta cgcagacctc
      421 ggtgcgacgg cgccgacgcc gtggccgaag tcgtcgtcga cgcgacgacg ttgccgtcgg
      481 aggagttcac gtccggagac tacagacggc cgaagccgcc gaccttgtat tcgacccgct
      541 cggtcgccca cggaatcgcc ttgaggcgct cctcgggaat cgccaggccg gaggcggcac
      601 agatgccgta ggtcttgttg gcgatgcgtt cgagcgcagc gtcgatttcc tcgaccgtcg
      661 atcgggcacg cgctgccagc tcgaggtcct ggtcgcgggc caccgccaag gtgtcgccct
      721 caccggactc ttcgtcgaat tgcacgtcgc cgggttcacg attcgcggtc agcgactgtg
      781 cctcttcgaa caggacctcg gcttgacgcg agtaggtcgc tcgctcgtcg agcagcatcg
      841 ccttttgcga ctccaggaac ttggcgtcgt agggcgatgt ggtcaggcgg ggctcgggat
      901 tcgcacccgg acgaggaagc gagcccaaca ccgacctgac cggagagacc ggcggggccg
      961 tctttgccgg tgccttggcc gtcgcggccg tctttgcggc cgtcttcgcc ggcgccttct
     1021 tggccggggc cttcgccgtc gcggccttct ttgccggagc cttggccggg gccttcgccg
     1081 tcgcggcctt ctttgcaggt gccttggccg gggccttcgc cttcgccgga gacttggtcg
     1141 ccgccttcgc cggagccttc ttggccggag ccttctttgc ggcgggagcc ttggccggag
     1201 ccttcttggc cggagccttc tttgcggcgg gagccttggc cggagccttc ttggccggag
     1261 ccgccttctt cgccgatgtc gctgccttgg acgtcgtcgc cttcttcgct ggcatgctcc
     1321 cccttcagtc tcggaacctg acaagccacc tgcgtcgttc gccggtcggc caggatgctg
     1381 actcgaaagc gaagatgcgc gggcggcagc atagacgccc cgcagacggt ttgcgactca
     1441 tctcctcgca gccaggtgat tcatcgcccg gcccaacggc tatcgacgac ggccgaagcg
     1501 acgcgtctga cgctcgtcgt cgccgccgaa gaaccgctcc atcgcctcgt cctcgccgcc
     1561 atcggagacg gcgagcgcct cgtgaaggtc ctgggcgtag cgatcctcac cgatctcggg
     1621 cagaccgacc gcggccacgg gttcgtcgcc cgcgaaggcg tcgacgccgt agtcgggctg
     1681 cggctcgggg gtgaacccgt cggaagtcat cgacaacccg ctgacctcgc gccagctccc
     1741 gggggcccat ggatcgctcg caccctggct ctggggctcc gacggggact cccatccctc
     1801 atcgaaaccc gtatcgaacc cggcatggtc aaccggcgac gcagcggggc tgtcctctcc
     1861 gaacgcctcg gcggccggaa gtgggctggg ggtgtcgaag tcgaccgggt cggtctcgac
     1921 gacgaacgaa ccgggatcga cactcgccga ctccgcctcc ggatcgagat actgcaacgg
     1981 aaccggcgca ccggcatcgg tgccataggc acccgattcc gcctcggcgc tctcggccga
     2041 atcgaaggtg tcggcgatcg ccggcgcagg ctgcgcctcg ccaggttccg cagcaactac
     2101 cggatcggag acggaggacc cggtttcggc actcgccagg aaggttgggc tctgcgggga
     2161 ggaagcgccg ccggtgcccg tgtcgacggc cgtatcggta gccgtgtcgc ggaactgcgc
     2221 aggcgccggg agctcgattc cgggcagcgg cggacgaagc atccgctcgg ggctgtcgag
     2281 cgccgcggtg atgtgatcga ccagcccgct caacgcagag cggtactcct ccacccgcgt
     2341 ctcgatggcg gcgagttggg tgacgcttga ggcccgctcc gaatcgagcg ttgcgatctc
     2401 cgcgagcacc tcgtcgcgcc gagcgccata ctcgcgtgcg gcctgctcgg acgcctcctg
     2461 agtcatctgg tcggccgacg cccgtgcggc gctgaccacg cggtccgcct cgactcgagc
     2521 atcgcgggtg atcctgtcgg cgttggtggt cgcgtcggcc acgagcttgg ccgcatcgga
     2581 cttggcctcc gcaaccgcgc gctccgcagt ctgcgacgcc aacatcaggg tccgcgccgc
     2641 ctcggcagcg gtcgcctcgt cggataccga cgaagggctc ggcgcaacca cggcctgcgc
     2701 cggcaccgcc gccgcagcct gcgctgcact caacgcctcg cgaaggcgca cgatctcctg
     2761 ttcggccgaa ccggcacgtg cgagcgcatc gtcgcgttcc aacccaagtg cgtcgagctg
     2821 accgctcagc tggagcaggt acgcgtcgac ctcatcgatg tcatagccgc ggaacttctc
     2881 gttgaagtca tacggaagtc gctgtggagt ctggtccatg cccgcatgct agcgacgtgg
     2941 tcgacaaaac cggcgcacgc cgagccgatt tcgtggcgag gtgccgtcgt gggcacccac
     3001 ggccattcag tcgtcgagaa tgccgcgctc gcgaaggcga cgacgctcct cgctcgacag
     3061 ttcgacgtcg gctggagtca gcaggtacac cgatgccgcc agccgttcga ccgagcctcc
     3121 gaggccatac gccacaccac tcgagaaatc gagcatccgg cgtgccaacc cacgatcgag
     3181 ttcgttcaga atcacgatca ccggctgatt cttcttgaag tggtcgccaa cctcttgggc
     3241 gtcgttgaac gagctcgggg tcaccgtgtg cggacgggcg ctggcgggag ttgccgggat
     3301 gggccgaacg gcgctggagc gcggacggac cggctcggcc gacgtggtgg tgcgagccgc
     3361 ggtgggcaac acccgaaccg ccgacggttc ggccgaatcg ccccagtcgt catcgggctc
     3421 gacgcggctc gacgccgctc gctgcgacgg cccacgcgcc ccgccggaac gaggaccggc
     3481 gccgcgttgg gcagggttgg gaaccgctcg caccaccggg ttcgaaggcg ccggcgcgac
     3541 ctgaggctcg tcgtagtaga cgtcgtcatc gtcgtaatcg tcgtagtcgt cgtagccgtc
     3601 atagccgtca tcgggaccca gccccagcca tgacattccc ttgcgcagca tccccgacat
     3661 caactcctcc tcggcaggcg tccacagtct tgcgcattga ggcggcacac aagcggacct
     3721 gagggcctac aacctactac gcagtgtcgt ggtcgccggg tgcgggcgtc gcttcaggta
     3781 ctccgaggcg aattgctgcc gcctggcggc gcgcctcagc gcggctccga tgggacgcga
     3841 actgcggttc cccgttcacc gtcgaggtcg cggtacacca cgcgaattcg tccgaaatct
     3901 cgtcaacccc gacaccggcg agcgcgactc tggcggccgc gacgacatcg agcgctggac
     3961 gggatgcgac ggtggcagcg atcacctcgg gaccgaaccg gaacgccatc gtcgtcaggt
     4021 cggcctcgga gaactcgtag tgcggcgccg aaatacaggg tccgatccac gctcgaatcg
     4081 ctcccgcgcc gagctcgcgc atcgactcaa ccgtgcgttc gatgatgccc tcatacagtc
     4141 cgcgccaacc tgcgtgggct gcggcgacca cctcggcacc gtcgaccgat gcgaacagga
     4201 ccggcacgca gtccgcgctc tgcaccgcga ccaccacgcc gggaaccgag gtgacgagcc
     4261 cgtcgccttc ggcgccgaga tggtcacccg gcgcgtcgac gatcatcacg cggtcgccgt
     4321 ggacctgacg caacgcagtc cacggccccg ccatcagacg ttgccgggaa cgcccgagga
     4381 caccggcctc ggagtcgatg gcgaagtcac cgtcggcggc ctcggagaac gcgaggctga
     4441 ccggctggcg ccccggtgat tcaaacgtcg cccgaagggc cacgggctac ttcaggaacg
     4501 aggggatgtc gaactcggtc tcgtcgtcgt catcgtcgtc accgaacaca tctgcgatgg
     4561 gcggctcgga ttcgacagaa cgcgaacgcc gttcgctcag gttcgacgga gcacggcgcg
     4621 tcggaaccgc agccttcgga tcttcccagc gctcgaatcc ggcgccgatc accgtcaccc
     4681 gcacctcgtc gcccatgtcg tcgtcgatgg tggtgccgaa gatgatgttg gcctcggcgt
     4741 gagccacctc gtgaacgacc gtcgcggcct cgttcacctc gtgcagggtg agcgcggaac
     4801 cgccgcagat gttcaacaag atgccgcgtg cgccgtcgat ctcgctttcg agcagcggcg
     4861 aactgatcgc ctggcgcgcc gccgcggtgg cacgaccttc gcccgagccg taaccgacac
     4921 ccagcagcgc ggtgccggcc gcactcatga tcatcttcac atccgcgaag tcggtgttga
     4981 tcagacccgg ggtggtgatc aggtcggtga tgccttgaac gccttgcaac aacacctcat
     5041 cggccatgcg gaacgcgtcg agcaccgagg tcttgtcgtt ggcaaccgac agaagccgat
     5101 cgttcggaat gacgatctgg gtgtcgacgt tctccttcaa cttggtgatg ccctgttcgg
     5161 cctgcaccga gcggcgacgc ccctcgaaca ggaacggacg ggtgaccacg gcgatcgtga
     5221 gcgctccgac cgtttccttg gcgatgttgg ccaccacggg agc
//
LOCUS       DAOOMA010000109         5256 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_18867_length_5256_cov_4.096904, whole genome shotgun sequence.
ACCESSION   DAOOMA010000109 DAOOMA010000000
VERSION     DAOOMA010000109.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 5256)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5256)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5256
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18867_length_5256_cov_4.096904"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1337)
                     /locus_tag="PLP95_04355"
     CDS             complement(<1..1337)
                     /locus_tag="PLP95_04355"
                     /inference="COORDINATES: protein motif:HMM:TIGR00196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HPB45066.1"
                     /translation="MIPVLSPAAMAAVDRHAADSLDELVERAGAAVAHAAIEMLGTVY
                     GRRVIVIAGSGNNGADGRVAAQILRTRGAAVEVREPSDRRKVKGFDLLIDAAFGTGFR
                     GTWDAPVTDGVAVLAVDIASGVNALSGAACGTPLRATRTVTFAAMKPGLFFADGPSLC
                     GEITLADIGLDLAPGDRVDRAPLHLLEADDLVALVPDRDRAAHKWNAAVLVVAGSPRM
                     PGAAALAAMGAQRGGAGYVRVASPGIDGPVGFAGAQGGHATLPVECVRVDAAEADWSD
                     DVLSECDRVRSIVLGPGLGRDQATGRAIRAVLRDASVPVVVDADALRAIGDIDSGFTE
                     LPENSVLTPHDGEFAAMADEPPGADRVADARDLAERTGAVVVLKGPTTVVAAPNGRAV
                     LVNAGDERLATAGTGDVLSGVIAALLARGANAFDAASAGALAHGLAARRLPLVG"
     assembly_gap    1467..1566
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1567..1864
                     /locus_tag="PLP95_04360"
     CDS             <1567..1864
                     /locus_tag="PLP95_04360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB45067.1"
                     /translation="HPVGWMPPMASTSDPAVTAIVDGTAVTLSHRSAAVLEALADGTV
                     VSREQLIRHAGLHDLSQRRCEGIIVELRKALGPDAIVNVRRRGWRLVTPVEITR"
     gene            complement(2008..2457)
                     /locus_tag="PLP95_04365"
     CDS             complement(2008..2457)
                     /locus_tag="PLP95_04365"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HPB45068.1"
                     /translation="MTPAHGLEPEGLEGLEGLEGLVGVVGVVAVGIDAVDVARMRSAL
                     DVTPSLIDRAFTESERAYALRQRDPAQRFAARWAMKEAVVKCLGGGVPGIDLRCIDVD
                     RGEGGEPSVRLSGRAAELAAQRGIVRWLVSMTHTDTTAMAIAVGLSA"
     gene            complement(2454..>5256)
                     /locus_tag="PLP95_04370"
     CDS             complement(2454..>5256)
                     /locus_tag="PLP95_04370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HPB45069.1"
                     /translation="YKAAFEIGELGDNTAELRRQIRSDALLHRVLSGPEVEALVLGHT
                     RWASVGIISEPNAHPQCSDELEATGGSLWTAVLNGDVDNHADLVADEDLKIAAAITTD
                     AKVIPALVSRRQMQGLDAVEAFRESVAVMEGSVAIAANDARDPQTLLLALRGSGQALY
                     VGLADDAYIVASEPYGVVEETVSYVRMDGETASNPDNSTASRGQVLRLDASGAGTTEG
                     ITRWSYDGTELPLTDDDVATAEITTRDIDRGDFPHFLLKEISDAPGSFRKTLRGKLSE
                     VDGRLRVTVPDSTLPQSVRDDLANGAITKILVIGQGTAAAAGSSLAVALSEFIAETPV
                     RVEAVLATELSGFGLRSDMSDTLVVAVSQSGTTTDTNRTVDLVRSRGAKVVAIVNRRN
                     SDLTDKSDGVLYTSDGRDIEMSVASTKAYYSQIAAGLVLATALADLVPGATSSDSPDR
                     QALLAALRELPAKMAETLERRDVIGAAAQRFAPSRRYWAIVGNGPNALAARELRIKLS
                     ELCYKSIACDATEDKKHIDLSAEPMILVCAAGLVGSTADDVSKEVAIYKAHKAAPIVI
                     ASDGENRFDAALAVIAVPETHERLAFVLSAMAGHLFGYEAALAIDAQANPLREARGVI
                     ENLVTDHAADLQHNDRATALLEGTAKELRPLAQTFMDGLRSGAYNGHLDASTAVRVSS
                     LFRYASGLSSLDAYQSEFGKIGTPGVVLDDLTSALTEGIEELTRPVDAIKHQAKTVTV
                     GISRSDETLLQVALVGDVLEAGAPRDRLSYATLRSLAELSPAVVDVVGFTRYRVENGE
                     HDDATAVVIDRGGVSLNLTSRTERDPRLRGTKHLVAREHELMVARGRGDGRTVLIVPE
                     TKDGQTTGLTLLHLRLVDRLPAATARAVLSGYRRRYQALRDAVTETEDHFRDDLLAEQ
                     DVLDLLCDPILDLADRWRA"
BASE COUNT          808 a         1817 c         1738 g          793 t
ORIGIN      
        1 cccaccagcg gcaggcgccg ggctgcgaga ccgtgggcca gggccccggc gctcgcggcg
       61 tcgaacgcgt tggcgccgcg agccaacagc gctgcgatca cccccgacaa cacgtcgccg
      121 gtgccggcgg tggccaggcg ctcgtcgccg gcgttcacca gaaccgcccg tccattgggt
      181 gcggcgacga cggtggtggg gcctttcagg accacgaccg cccccgtgcg ctcggcgaga
      241 tcgcgggcat cggccacacg atcggccccg ggaggctcgt cggccatcgc cgcgaactcg
      301 ccgtcgtgag gggtcagcac agagttctcc ggaagttccg taaaccccga gtcgatgtcg
      361 ccgatcgccc gcaacgcgtc ggcgtcgacg acgaccggga ccgaggcgtc gcgcagcacc
      421 gcccggatcg cccgaccggt ggcttggtcg cgtcccaacc cggggcccaa cacgatggaa
      481 cgcacgcggt cgcattcgct cagcacgtcg tcggaccagt ccgcctcggc agcgtcgacg
      541 cgcacgcatt ccaccggcag tgttgcgtgg ccgccctgag cgccggcgaa accgaccggg
      601 ccgtcgatgc cgggagacgc cacccgcaca tatccggcac cgccgcgctg ggcccccatc
      661 gccgcaagcg ctgccgcacc cggcattcgc ggtgaaccgg cgacgaccag gaccgcagcg
      721 ttccatttgt gcgcggcgcg atcgcggtcg ggtaccaacg cgacgaggtc gtcggcctcg
      781 agcaggtgca gcggcgcacg atcgacgcgg tcgcctgggg cgagatccaa cccgatgtcg
      841 gcgagggtga tctccccgca caacgacgga ccgtcagcga agaacagtcc gggcttcatg
      901 gccgcgaacg tcacggtgcg cgtcgcacgc aacggcgtgc cgcaggctgc tccactcagt
      961 gcgttgaccc ccgaggcgat gtcgaccgcc agcaccgcaa cgccgtcggt caccggcgca
     1021 tcccaggtgc cgcgaaaccc cgtaccgaac gcagcatcga tgagcaggtc gaaccccttc
     1081 accttgcgcc gatcggaggg ttcgcgaacc tccacggccg cgccgcgggt gcggaggatc
     1141 tgtgcggcca cccgaccgtc cgctccgttg ttgcccgacc cggcgatcac gatcacccgt
     1201 cggccgtaga cggttccgag catctcgatc gcggcgtggg cgaccgcagc accggcgcgc
     1261 tcgaccagtt cgtcgagcga gtcggcggca tgacgatcga ccgcggccat ggcggcagga
     1321 ctcagcacag gaatcacccg gacattctgc ccgcttcgtc aacacctcga catcgttcat
     1381 cgaggcgtaa caatgagcgc gcgaacgcga aacacccggt tggttggatg cctcccatgg
     1441 cttcgacctc cgatcccgcc gtcaccnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1501 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1561 nnnnnnacac ccggttggtt ggatgcctcc catggcttcg acctccgatc ccgccgtcac
     1621 cgccatcgtc gacggcaccg ccgtcacgtt gtcccatcgc agcgccgccg tattggaagc
     1681 cctcgccgac ggcacggtgg tgagccgcga acagctcatc cgccatgccg ggctgcacga
     1741 tctgtcgcag cgacgatgcg agggcatcat cgtcgagctc cgcaaggcgc tcggacccga
     1801 tgccatcgtg aatgtgcgcc gccgcggctg gcggttggtc accccggtgg agattacgcg
     1861 ctgacccgcc ccggcgcccg ccagcccgcc agccgttctg cccgccaggc gttctgtagg
     1921 taatcggtgt tgctatagca acaccgatta cctacagaac gggccggggc ggcggggccg
     1981 ggtcggggcg gggccgggac ggcggggtca ggcgctcagg cccaccgcga tcgccatggc
     2041 cgtggtgtcg gtgtgggtca tcgacaccaa ccaacggacg atgccgcgtt gcgccgcgag
     2101 ttcggccgct cggccgctca gccgcaccga cggctccccg ccctcgcccc ggtcgacgtc
     2161 gatacacctc aggtcgatcc caggaacgcc ccctccgaga cacttgacca ccgcctcctt
     2221 catcgcccat cgggcggcga agcgttgcgc cgggtcgcgc tggcgcagcg cgtaggcgcg
     2281 ttcggactcg gtgaaggccc ggtcgatcag gctgggggtc acgtcgagcg ccgatcgcat
     2341 gcgtgcgaca tcgaccgcgt cgatccccac cgccacgaca cccacgacac ccacgaggcc
     2401 ctcaaggccc tcaaggccct caaggccctc aggttcgaga ccgtgggccg gcgtcatgcc
     2461 ctccagcggt cggccaggtc gaggatcggg tcgcacaaca gatcgagcac gtcctgttcc
     2521 gccaacaagt cgtcgcggaa atggtcctcg gtctcggtca ccgcatcgcg cagcgcttgg
     2581 tatcgacggc gatagcccga caacaccgct cgggcggttg ccgccggaag ccgatcgacc
     2641 agacggaggt gcaacaacgt cagcccggtg gtttggccgt ccttggtttc gggcacgatc
     2701 aacaccgtgc gtccgtcgcc gcgcccacgc gccaccatga gttcgtgctc tcgtgccacg
     2761 aggtgcttgg tgccgcgcag ccgaggatcg cgctcggttc gcgaggtcag gttcagcgag
     2821 accccgccgc gatcgatgac caccgcggtc gcatcgtcgt gctcgccgtt ttcgacccgg
     2881 tagcgggtga agccgaccac gtcgaccacc gccgggctga gttcggcgag cgaccgcaac
     2941 gtcgcgtagc tgagccgatc acgcggcgct cccgcctcga gcacatcgcc gacgagcgcc
     3001 acctgcaaca gcgtctcatc ggaacggctg atgccgacgg tcacggtctt ggcctgatgc
     3061 ttgatcgcgt cgaccgggcg ggtgagttcc tcgatcccct cggtcagcgc cgaggtgagg
     3121 tcgtcgagca cgaccccggg ggtgccgatc ttgccgaact cgctctggta ggcgtcgagg
     3181 ctcgacagcc ccgacgcgta gcggaacagc gacgacaccc gcaccgcggt cgacgcgtcg
     3241 aggtgaccgt tgtaggcacc cgatcgcagg ccgtccatga aggtctgggc gagcggccgc
     3301 agttccttgg cggtgccttc caacagcgcc gtggcccggt cgttgtgttg cagatcggcc
     3361 gcgtggtcgg tgaccaggtt ctcgatcacc cctcgcgcct cacgcaacgg attcgcctga
     3421 gcgtcgatcg cgagggccgc ctcgtagccg aacaggtgcc cggccatggc cgacagcacg
     3481 aacgccaggc gctcgtgggt ttcaggcacc gcgatcaccg cgagcgcagc gtcgaaacgg
     3541 ttctccccgt cggacgcgat gacgatcggc gcagccttat gggccttgta gatcgccact
     3601 tccttgctga cgtcatccgc ggtcgagccg accaggccgg ccgcacacac gaggatcatc
     3661 ggttcggcgg acaggtcgat gtgtttcttg tcctcggtgg cgtcgcacgc gatcgacttg
     3721 taacacagct ccgacaactt gatgcgcagc tcgcgggccg ccagggcgtt gggcccgttg
     3781 ccgacgatgg cccagtagcg acgagacggg gcgaagcgct gggccgcggc accgatgacg
     3841 tcgcgccgct cgagggtctc ggccatcttg gcgggaagtt cacgcagcgc ggccaacagg
     3901 gcctggcgat ccggcgaatc tgaactcgtc gcgcccggga cgaggtccgc cagcgcggtg
     3961 gccagcacca ggccggccgc gatctggctg tagtaggcct tggtggaggc caccgacatc
     4021 tcgatgtcgc ggccatcgga ggtgtagagc acgccgtcgg atttgtcggt gaggtcgctg
     4081 tttcgccggt tgacgatcgc gacgaccttg gcgccgcggg agcgcacgag gtcgaccgtg
     4141 cggttggtgt cggtggtggt gcccgattgg ctgaccgcca cgaccaacgt gtcgctcatg
     4201 tcgcttcgga ggccgaaccc cgacagttcg gtggcgagca ccgcctcgac gcgaacgggg
     4261 gtctccgcga tgaactcgct gagggctacc gccagcgagg atcccgccgc ggccgccgtg
     4321 ccttggccga tgacgaggat cttggtgatg gcgccgttcg ccaggtcgtc gcgcacagat
     4381 tggggaagcg tcgagtcggg aaccgtgacc cgcaaacgac cgtcgacctc ggagagcttg
     4441 ccgcggaggg tcttgcggaa cgacccgggg gcatcggaga tctccttcaa caggaaatgc
     4501 gggaaatcgc cgcggtcgat atctcgggtg gtgatctcgg cggtcgccac gtcgtcgtcg
     4561 gtcaacggca actcggtgcc gtcatagctc caacgggtga tgccttcggt ggttccggcg
     4621 ccgctggcgt cgaggcgcag cacctggccg cggctcgccg tcgagttgtc ggggttcgac
     4681 gcggtctcgc cgtccatacg gacatagctc acggtctcct caaccacgcc gtaaggctcg
     4741 gaggcaacga tgtaggcgtc gtccgcgaga cccacataga gggcctggcc gctgccgcgc
     4801 agcgccaaca acagggtctg ggggtcgcgc gcatcgttcg ctgcgatggc gacggagccc
     4861 tccatcaccg ccaccgactc acggaacgcc tcgaccgcat cgaggccctg catctgacga
     4921 cggctcacca gcgcggggat gaccttggcg tcggtggtga tcgccgccgc gatcttgagg
     4981 tcctcatcgg ccacgagatc ggcatggttg tcgacgtcgc cgttgagcac cgccgtccac
     5041 aggctcccgc cggtcgcttc gagttcatcg gagcactgcg ggtgggcgtt gggttcggag
     5101 atgatgccca ccgaggccca tcgggtgtgg cccaacacga gcgcctcgac ctcggggccg
     5161 ctcaggacgc ggtgcaacag cgcatcggat cggatctggc ggcgcagctc cgcggtgttg
     5221 tctccgagtt caccgatctc gaacgcggcc ttgtac
//
LOCUS       DAOOMA010000110         5242 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_18942_length_5242_cov_3.893580, whole genome shotgun sequence.
ACCESSION   DAOOMA010000110 DAOOMA010000000
VERSION     DAOOMA010000110.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 5242)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5242)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5242
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18942_length_5242_cov_3.893580"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..766
                     /locus_tag="PLP95_04375"
     CDS             <1..766
                     /locus_tag="PLP95_04375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB45070.1"
                     /translation="WASGGRASARRLLEACKAGAATLHGTGVHPSYMVERLVLTMCQP
                     LDSVSHIRFVEAANFQGAPGDMWGGLGAMGFGVDIDEIGPQHPVAAGGDLYYGDVIGN
                     VARELFDADPAEVRVERSYRGIPAEQDVVIGNETIRRGTAGAVHLVHRGYLGDHHFFT
                     NEECWYLRDAVTFRGDDLPFGGFTTSASYTIEITGEPANLRSQLEFQPTLKGRDAITN
                     GSVRAVLAAIPAVCAAEPGILVDDSAPSYRLISADD"
     gene            complement(838..2052)
                     /locus_tag="PLP95_04380"
     CDS             complement(838..2052)
                     /locus_tag="PLP95_04380"
                     /EC_number="2.8.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024860.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA];
                     GO_function: GO:0050656 - 3'-phosphoadenosine
                     5'-phosphosulfate binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HPB45071.1"
                     /translation="MWQPPERTAAASAAIAAADQGRLARPDRYLLDADHLASVAARRD
                     GNTERFAAGWREGLERYVESASEDGRLNAFGTAMAARTAVSRLRSGVALSRFREANPN
                     VADRPPTPPIFITGGWRSGTTFLFRLLASDPHLRAPLPAELAEPMSMASLSGEERERR
                     IDASAAAHDMLHALNPTLRAIHDSGARLPEECGLAMGADLRSWSLSATTRLESYSEWL
                     AGQDLGPSYRDYASVLGVLGDGDDRRWVLKAPPHLAELGALAQAFPGAVVVILHRDIV
                     ETIASGASLFAVFRSTYSDDVDPRDVGRFQSDQTELWMRRAHEFRTSALASTITMLDV
                     DYGDLVSDPAGTLDRVYAAADREPPADLEAFVADFNAAHPRHAQGTHRYSPEDFGLNP
                     AELRERFAEFAR"
     gene            complement(2055..3734)
                     /locus_tag="PLP95_04385"
     CDS             complement(2055..3734)
                     /locus_tag="PLP95_04385"
                     /inference="COORDINATES: protein motif:HMM:NF018452.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HPB45072.1"
                     /translation="MATTPRRRLSAEARRDQLLDACAAVVDAEGFPAVSIDAIAAACD
                     VTRTVLYQQFGGLEGMLEALVERSTLRAAAAIDTTGTATGARTLLDSMAHVLDAVDAD
                     PATWRMFLIAPNVGPRSLSDNLAAGRALLRTQLAGMIEQRTGDADADLTARMLQAAAD
                     ELVRLRLADPDRYPTERLLDQFESLADALAGPAQPAEPAQPAEPHRGWTALVDGLRSA
                     GDQVANLAETGEADDQSDAFRALLRGLTNQLGRFEVDRDRPELVAFNGWREKMFMDNP
                     DFRYWVADVRDDRRYRITGNIADAVYMSVTAYSSSGTLDASAGARLDSDTIRLADDGE
                     FTITMSSEDPGDGTDWLALPPRANAVWVRQFHRDVANETHGWCLIDPLDSPPPPARSL
                     DSERFGTKLTRLGERLAAFPAIWAAATSDDFNAPNTVRHWTEMAGGAAFTEPGIHYLR
                     GSWQLEPGEALVIEGTPPPCRYWNVMLYDRFLNSLDYRARTVSYTGATATLSDGAYRF
                     ILAGDDPDGSGDWLDTEGRSFGLFVMRFLQPAVEPPLPHLRPYPIEHLRER"
     gene            complement(3791..>5242)
                     /locus_tag="PLP95_04390"
     CDS             complement(3791..>5242)
                     /locus_tag="PLP95_04390"
                     /inference="COORDINATES: protein motif:HMM:NF015094.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/UbiB family protein"
                     /protein_id="HPB45073.1"
                     /translation="TTMNDSIRGPLSNGPTPDMLEVDAPPLHEFRLGDVARLIVVFFV
                     IATSTLNALARRGLRIVGRTVSVKLGRTPSTEPVAGLIDAACNGLIDGFIRLGPTFVK
                     LGQIIASSGALFPDQLVAAARRCLDQVPTFPAEVARATIAEDLGHPIESVFAAFVDEP
                     LSAASIGQVHAVTLHDGREAVVKVQRPGIREQMAGDLRNAARVAWLFEKTPWGKASGA
                     VAIIEDLHSVTFQELNPVREAWQQDRFRSNIWAFGDNTMITAPEVYWDFCGRRTICME
                     RVSGMPMDRFDELAERGLDLQLVVRRGAKVWAEAAMIHGPFHGDMHAGNIWVLDDGRG
                     CFLDFGIMGELSGEWQDLMKDLFYTCAIDLDFRRVVESYRRVGAIPAEFSDDTQLSMF
                     LTNVLGTMLADGFGGVDIGALVTESVKMLKTYNASVPRELVLIAKQLLYIDRYTKHLA
                     ADYSITSDPFIIKNVFPEEAAKKAADLGVAMPI"
BASE COUNT          845 a         1666 c         1851 g          880 t
ORIGIN      
        1 ctgggcgtcg ggtggccggg catcggctcg gcggctgctc gaggcgtgca aggccggtgc
       61 cgcgacgctg cacggcacgg gagttcaccc ctcctacatg gtcgagcgac tggtgctgac
      121 catgtgccaa ccactcgaca gcgtgagcca catccggttc gtcgaggccg cgaacttcca
      181 gggcgccccc ggcgacatgt ggggtgggct cggtgcgatg ggcttcgggg tcgacatcga
      241 cgagatcggt cctcaacatc cggtggccgc aggaggcgat ctctactacg gcgatgtcat
      301 cggcaacgtc gcccgtgaac tcttcgacgc ggatccggcc gaggtgcgcg tcgagcgcag
      361 ctaccgcggc attccggccg aacaggacgt ggtcatcggc aacgagacga ttcggcgcgg
      421 caccgcggga gcggtccacc tcgtgcaccg cggctatctc ggagaccacc atttcttcac
      481 caacgaagag tgctggtacc tgcgcgacgc ggtgacgttt cggggggacg atctcccctt
      541 tggcggattc accacctccg cctcctacac gatcgagatc accggcgaac cggcgaatct
      601 gcgttcacaa ctcgagtttc agccgacact caaggggcgc gacgcgatca ccaacggatc
      661 ggtacgcgcc gttctcgccg ccatcccggc cgtatgtgcc gcggagccag gcattcttgt
      721 ggacgactcg gcccccagct accggttgat ctccgccgac gactgacctc caccgacgac
      781 tgagccccgc cgacatctga ggtcctgcgc gagggaggcc tgcgcgaccg aggcccgtca
      841 gcgggcgaac tcggcgaacc gttcacgtag ctccgcgggg ttgagcccga agtcctcggg
      901 ggagtagcgg tgcgttccct gtgcatgtcg ggggtgtgcc gcgttgaagt cggcgacgaa
      961 agcctccagg tcggccgggg gttcccgatc ggccgcggcg tacacccgat cgagggtgcc
     1021 cgcagggtcg gagacgagat cgccgtagtc gacgtcaagc atcgtgatgg tgctggccaa
     1081 ggcgctggtg cggaactcgt gcgcccggcg catccacagc tcggtctgat cgctctgaaa
     1141 ccgtccgacg tctcgggggt cgacgtcatc gctgtaggtg gaacggaaca ccgcgaacag
     1201 gctggctccc gacgcgatcg tctcgacgat gtcgcgatgc agaatcacca cgaccgcgcc
     1261 gggaaacgcc tgagcgagcg caccgagctc cgcgaggtgt gggggagcct tcagcaccca
     1321 gcggcgatcg tcgccgtcgc cgagcacgcc cagcaccgac gcgtagtccc gataggacgg
     1381 acccaggtcc tggcccgcaa gccactcgga gtacgactcg agccgggtgg tcgccgagag
     1441 tgaccagctt cgaaggtcgg ctcccatagc gagcccgcat tcctcgggca gccgcgcccc
     1501 ggaatcgtga atcgctcgta gcgtggggtt gagggcatgc aacatgtcgt gggctgcggc
     1561 gctggcatcg atgcgccgct cgcgttcctc gccgctgagg ctcgccatcg acatcggttc
     1621 ggcgagttcg gctggcagcg gcgcgcgcaa gtggggatcg gaggccagga gccgaaacag
     1681 aaacgtcgtt ccgctgcgcc acccgccggt gatgaagatc ggtggcgtcg gcgggcgatc
     1741 ggcgacgttc gggttggctt cgcgaaagcg cgagagcgcc accccggagc gaagacgcga
     1801 gacggcggtg cgcgctgcca tcgccgttcc gaacgcgttg agccgtccat cctcgctcgc
     1861 cgattccacg tagcgttcga ggccctctcg ccagccggcg gcgaaccgtt cggtgttgcc
     1921 atctcggcgg gctgcgacgc tcgcgaggtg atcggcgtcg agcagatacc gatccggacg
     1981 cgccaggcgg ccctggtcgg cagcggcgat cgcagcgctc gcggcggcgg ttcgttcggg
     2041 cggttgccac atggtcaacg ctccctcagg tgttcgatcg ggtacggtcg aaggtgcggt
     2101 agcggcggct cgacggcggg ttgcaggaac cgcatgacga acagcccgaa cgagcggccc
     2161 tcggtgtcga gccaatcgcc ggagccgtcg gggtcgtcgc cggcgaggat gaatcggtag
     2221 gcgccgtcgc tcagggtggc ggtggcgccg gtgtaggaca cggtgcgcgc ccggtagtcg
     2281 agtgagttga ggaaccggtc gtacaacatc acgttccagt agcgacacgg cggtggtgtg
     2341 ccctcgatca cgagcgcctc gcccggctcg agttgccacg agccccgaag gtagtgaatg
     2401 ccgggttcgg tgaacgccgc gccgccggcc atctcggtcc agtggcgcac ggtgttcggt
     2461 gcgttgaagt catcgctggt ggcggccgcc cagatggcgg gaaacgccgc cagtctttcg
     2521 ccgaggcggg tgagtttggt gccgaatcgt tcgctgtcga gcgaccttgc gggcggcgga
     2581 ggggaatcga ggggatcgat gagacaccag ccgtgtgtct cgttggcaac gtcgcggtgg
     2641 aattgtcgga cccacactgc gttcgcgcga ggcggcaagg cgagccagtc ggtgccgtcg
     2701 cccgggtcct cgctggacat ggtgatggtg aactcaccgt cgtcggcgag tcggatcgtg
     2761 tcggagtcga ggcgtgcgcc ggccgatgcg tcgagggtcc ccgacgagga gtaggccgtc
     2821 accgacatgt acaccgcatc ggcgatattg ccggtgatcc ggtagcggcg gtcgtcgcgg
     2881 acgtcggcca cccagtaacg aaagtcagga ttgtccatga acatcttctc gcgccatccg
     2941 ttgaaggcga cgagttcggg gcgatcgcgg tcgacctcga agcgcccgag ctgattggtc
     3001 aagccgcgca gcagtgctcg aaacgcgtcc gattggtcgt cggcctcacc ggtttcggcg
     3061 aggttcgcca cctggtcgcc ggccgagcgc agcccgtcga ccagtgcggt ccagcctcgg
     3121 tggggttcgg ccggttgggc gggttcggcc ggttgggctg ggcctgcgag tgcgtcggcc
     3181 agcgactcga actgatcgag gaggcgttcg gtggggtagc ggtccggatc ggcgagtcgg
     3241 agtctgacta gttcgtccgc ggcggcctgc agcatgcgcg cggtcagatc ggcatcggcg
     3301 tcaccggttc gttgttcgat cattccggcg agctgtgttc gcagcagcgc tctgcccgct
     3361 gcgaggttgt cgctcaggct gcgcggcccg acgttcgggg cgatgaggaa cattcgccac
     3421 gtcgccgggt cggcgtcgac cgcgtcgagc acatgggcca tcgagtcgag cagcgttcgc
     3481 gccccggtgg ccgttcccgt cgtgtcgatg gctgccgccg cccgcagtgt cgagcgttcg
     3541 acgagcgcct cgagcatgcc ctcgaggcca ccgaactgtt ggtagagcac cgtgcgggtg
     3601 acatcacacg ccgccgcgat ggcgtcgatg gacacggccg ggaaaccctc ggcgtcgacc
     3661 acggcggcgc atgcgtcgag gagttggtcg cggcgtgctt cagcgctgag ccgacgtcgt
     3721 ggggtcgttg ccatggcgac acgctagaga cctcacccgc atctttcaat gtgtaagttg
     3781 tgcggttcgg tcagatgggc atcgcgaccc cgaggtcggc ggccttcttg gcagcttcct
     3841 cggggaacac gttcttgatg atgaacgggt cagaggtgat ggagtaatcc gccgccaggt
     3901 gtttggtgta gcggtcgatg tagagcaact gcttggcgat cagcaccagc tcgcggggta
     3961 ccgatgcgtt gtaggtcttg agcatcttca ccgactcggt caccagcgcc ccgatgtcga
     4021 cgccgccaaa gccgtcggcc agcatcgttc ccaagacgtt ggtcaggaac atcgacagct
     4081 gagtgtcgtc gctgaattcc gccgggatgg cgccaacacg gcggtacgac tccaccaccc
     4141 gtctgaaatc gaggtcgatg gcgcaggtgt agaacaggtc cttcatgagg tcctgccact
     4201 cgccggacaa ctcgcccatg atcccgaaat ccaggaaaca accccggccg tcgtcgagca
     4261 cccagatgtt gccggcgtgc atgtcgccgt gaaacgggcc gtggatcatc gccgcttcgg
     4321 cccacacctt cgcgccgcgg cgcacgacga gttgcagatc gagcccccgt tcggcgagtt
     4381 cgtcgaaacg gtccatcggc atgccggaga cccgttccat gcaaatggtg cgacggccgc
     4441 agaagtccca gtacacctcg ggtgcggtga tcatcgtgtt gtcgccgaag gcccagatgt
     4501 tggaacggaa ccgatcctgc tgccacgcct cgcgcaccgg gttgagctcc tggaacgtca
     4561 ccgaatggag gtcttcgatg atcgcgacgg cgccgctcgc cttaccccag ggggtctttt
     4621 cgaacagcca agcgactcgg gccgcgttgc ggaggtcgcc cgccatctgc tcgcggatac
     4681 cggggcgctg aaccttgacg acggcctcgc ggccgtcgtg gagcgtcacc gcgtggacct
     4741 ggccaatcga cgctgcggac agtggctcgt cgacgaacgc cgcgaacacg ctttcgatcg
     4801 gatgcccgag gtcctcggcg atcgttgccc tcgcgacctc ggcgggaaac gtcggcacct
     4861 ggtcgaggca ccgacgtgcg gcggcgacca actggtcggg aaacagcgca ccggaggacg
     4921 cgatgatctg tccgagtttc acaaacgtcg gcccgaggcg gatgaatccg tcgatcaggc
     4981 cgttgcaggc cgcatcgatg aggccggcga cgggctcggt ggacggggtg cgaccgagct
     5041 tgacgctgac ggttcggccg acgatgcgca atccgcgccg cgcgagcgcg ttcagcgtgg
     5101 acgtggcgat gacgaagaac acgacgatga ggcgcgccac gtcgccgagg cggaactcgt
     5161 gaagcggcgg cgcgtcgacc tccaacatgt cgggggtcgg cccgttgctg agtgggccgc
     5221 gaatcgaatc gttcatggtg gt
//
LOCUS       DAOOMA010000111         5221 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_19045_length_5221_cov_3.634727, whole genome shotgun sequence.
ACCESSION   DAOOMA010000111 DAOOMA010000000
VERSION     DAOOMA010000111.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 5221)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5221)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5221
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19045_length_5221_cov_3.634727"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..485)
                     /locus_tag="PLP95_04395"
     CDS             complement(<1..485)
                     /locus_tag="PLP95_04395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB45074.1"
                     /translation="MSATTRRLRSLALDRTFGGGFAGFAASVAIGWGIDQLRKVGDPV
                     TIDATDLRPGEQYVVTARPAMKRKERKVFAKQQAARRRLNTATAPSRSTVRVARKLSR
                     AQRQADRRPTGSPRQQRAVRRSERLGAAYDRRVVPSRKVARLQRNADALDAELTALRA
                     A"
     gene            complement(482..688)
                     /locus_tag="PLP95_04400"
     CDS             complement(482..688)
                     /locus_tag="PLP95_04400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB45075.1"
                     /translation="MSTEKITPDDIKAKLSAIQDEATGTVEGAKSQLITVGGIVALGV
                     LVIAFILGRRVGVRKNTIIEVRRA"
     gene            complement(685..1032)
                     /locus_tag="PLP95_04405"
     CDS             complement(685..1032)
                     /locus_tag="PLP95_04405"
                     /inference="COORDINATES: protein motif:HMM:NF018980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HPB45076.1"
                     /translation="MADANAPRTKPADHVNELKGLVVGYAKQETVDPLKSLGRYLGYG
                     LGGAFLVGVGMVFLLMALLRGLQSAPWFDHNSGAASLVPYAATFVAAIIVIAVAGYLG
                     FKNDPNKKKDAAS"
     gene            complement(1069..1737)
                     /locus_tag="PLP95_04410"
     CDS             complement(1069..1737)
                     /locus_tag="PLP95_04410"
                     /inference="COORDINATES: protein motif:HMM:NF014269.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LON peptidase substrate-binding
                     domain-containing protein"
                     /protein_id="HPB45077.1"
                     /translation="MTDAVDGDSDPIGTTDPESLPMFPLGTVLMPTGLLPLRLFEPRY
                     LRFIEDVLAAGNGFGVVLIERGSEVGGGDQRCMIGCRAVVHRADQALDGTWQVLAIGT
                     ERIEVVDWLADDPYPRALVRPLPDRSSPVAADDDPRWLELDTSFRHLVRLVSERTGRG
                     PIEEIELADEPALATFQMAAVLPLNPFDRQRILASCDVDERRRILLECFEDLAVLVSG
                     EENH"
     gene            complement(1734..3167)
                     /gene="dacB"
                     /locus_tag="PLP95_04415"
     CDS             complement(1734..3167)
                     /gene="dacB"
                     /locus_tag="PLP95_04415"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:NF014200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004175 - endopeptidase activity [Evidence
                     IEA];
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine
                     carboxypeptidase/D-alanyl-D-alanine-endopeptidase"
                     /protein_id="HPB45078.1"
                     /translation="MGAGMVVAGLLVGGWEWSDESARSDAATPATSSAANSPSATTAA
                     LSARRVPKFLVAPQADRRLNNAIAPKLAALSDTSCFEVRDGERVIVSHGTDQALAVAS
                     NQKLLTAWVALEVLGADHTFTTRFTTTAPIDGGVINGDLWIIGGGDPLLESDTYRSTF
                     KWGKGAHTSIEAIADELAALGITAITGSVVGDDSFFDAERTVSTWPSRMLAQGQVGPL
                     SALSVNDARTYSPIPGAGGGAQPASDPAAWAADAFTQLLTARGIAVGGAPASGQAPET
                     TNTVLEIASRPVWQLVAQMLTFSDNNTAELLVKAIGREVSGEGSTAAGLVAMVDRLGG
                     LGTDMSQISLLDGSGLDDGSRVTCDLILATLNGDDSDPDPSQGVLAAGLPTANGEVGT
                     LRDRFHNSPGAGRVFAKTGTLRSVTALSGWAQAREERISFATVINTVGREVTGADTNA
                     EDALAAALIDYPDSIDPALVGPSVPRP"
     gene            complement(3343..4818)
                     /locus_tag="PLP95_04420"
     CDS             complement(3343..4818)
                     /locus_tag="PLP95_04420"
                     /inference="COORDINATES: protein motif:HMM:NF024994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HPB45079.1"
                     /translation="MPTDEQFKALFEPRGVLIAGASTHPGKFGFVSLHNVLACGFPGK
                     VFATNRDGAEVLGVQSVPDIDELPAGEIDLVFVCTPKDANPDLLRKCAAKGVKAAFLT
                     SAGYGEAGEQGRRDEADLVALCEELGILLAGPNGQGVVSTPAQLCAQIVAPYPPAGPI
                     GVASQSGNFISSFLNLSLATGVGISRAVSAGNAAATGVADFLGFYESDPATKVGLAYV
                     EGLEDGRGFFDAVRQITPTMPVVVVKGGATAGGAKAASSHTGSLATDDRVFDGACRQG
                     GLTRARTVEEAFEAAATLATQPLPAGNRVVVMTTAGGWGVVTADAISASRTLELVELP
                     DDLLAAIDEHLPPRWSRNNPVDLAGGETRDTIPTVMELIARHPSVDSIVYLGLGIQSN
                     QAAMMVDGPFYPDHGLERIVEYHRRQDARFAHAAAEISEATGKPILCCTELAISDPTN
                     AGPAAVRASGRVCYATANRAVTALEHLTRYAEFRRHHEQNA"
     gene            complement(4818..>5221)
                     /locus_tag="PLP95_04425"
     CDS             complement(4818..>5221)
                     /locus_tag="PLP95_04425"
                     /inference="COORDINATES: protein motif:HMM:NF024938.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HPB45080.1"
                     /translation="DGEVGVLVAAMISGTRELIAGVARDPQFGLTVMVGIGGVLAEAI
                     GDVAVGLVPITTADAHEMIDALESVALLGEFRGEPAVDREALAAVLVALSDAAASNPA
                     VHSIDLNPLIIANGTPIAVDALVELTTEGTR"
BASE COUNT          800 a         1736 c         1806 g          879 t
ORIGIN      
        1 ttcgccgcgc gcagtgcggt gagttcggcg tcgagggcat cggcgttgcg ttgaagcctc
       61 gcgaccttgc ggctcggtac gacacggcgg tcataagccg ccccgaggcg ttccgagcgg
      121 cgcaccgccc gctgctggcg aggtgagccg gtgggacgcc gatcggcctg gcgttgtgca
      181 cgcgaaagct tccgggcaac gcgaaccgtc gaccgcgacg gcgccgtggc ggtgttgagg
      241 cggcgacgag cggcctgctg tttggcgaag acctttcgtt ccttgcgttt catcgcgggt
      301 ctcgcggtca cgacgtactg ctcgccgggg cgcaggtcgg tcgcatcgat agtgaccgga
      361 tcgccgacct tgcgaagctg gtcgattccc caaccgatgg caaccgacgc ggcgaagcct
      421 gcgaatcctc cgccgaaggt tcggtcgagc gcgagggaac ggagccgtcg cgtcgtggcg
      481 ctcacgcgcg cctcacctcg atgatcgtgt tctttcggac cccgacccgg cggccgagga
      541 tgaaggcgat gaccaacaca cccaacgcga cgatgccgcc gacggtgatc aattggcttt
      601 tcgcgccttc gaccgtgccg gtggcctcgt cctgaatcgc cgacagcttg gccttgatgt
      661 cgtcgggggt gattttctcg gtgctcacga cgctgcgtct ttcttcttgt ttggatcgtt
      721 cttgaagccc agatagccgg cgacggcgat gacgatgatg gcggcgacga aggttgccgc
      781 atacggcacg agcgacgccg caccgctgtt gtggtcgaac catggcgccg actgcaggcc
      841 gcgaagcaac gccatcagca agaacaccat cccgaccccc acgagaaacg ccccgccgag
      901 cccgtagccc aggtatcgcc cgagggattt gagcggatcg acggtctctt gtttcgcgta
      961 gccgacgacg agtcccttca attcgttgac gtgatcggcc ggcttcgtgc gtggtgcgtt
     1021 ggcatcagcc atcggttctc ctcggattcg tcacgacggc ttcctttcct agtggttctc
     1081 ctcgccgctc acaagcacgg cgaggtcctc gaaacattcg agcaggatgc ggcgacgctc
     1141 atccacgtcg catgatgcca ggatccgttg gcgatcgaac gggttgagcg gcaggaccgc
     1201 ggccatctga aaggtcgcca gcgccggttc gtcggcgagt tcgatctcct cgatcgggcc
     1261 gcgaccggtt cgttcggaca cgagtcgcac caaatggcga aacgacgtgt ccagctccag
     1321 ccaccgcggg tcgtcgtcgg ccgctaccgg cgaggaacgg tccggcagcg gtcgaacgag
     1381 tgcccgcggg taggggtcgt cggccagcca gtcgaccacc tcgatgcgtt cggtgccgat
     1441 cgccaggacc tgccacgtgc catcgagggc ctggtcggct cgatgcacga cggcgcgaca
     1501 gccgatcatg caccgttggt cgccgccgcc cacctcgctg ccgcgctcga tcaacacgac
     1561 gccgaacccg ttgcccgccg cgagcacatc ctcgatgaaa cgcagatagc ggggttcgaa
     1621 caggcgaagt ggtagcaatc cggtcggcat gaggacggtt ccgagcggaa acatcggcag
     1681 cgactcgggg tcggtcgtgc caatcgggtc gctgtccccg tcgacggcat cggtcatggg
     1741 cgcggcacgc tcgggccgac gagcgccggg tcgatcgaat cgggatagtc gatcagcgcg
     1801 gccgccaacg catcttcggc gttggtgtcc gcgccggtca cctcgcgccc gacggtgttg
     1861 atcaccgttg cgaagctgat tcgttcctcg cgcgcctggg cccagccgct caacgccgtg
     1921 accgatcgca gcgtgcccgt cttggcgaag acccgtccgg ccccagggct gttgtggaac
     1981 cggtcgcgca aggtgcccac ctctccattg gcggtcggca accccgcggc gagcacgccc
     2041 tgggacgggt cggggtcgga gtcgtcgccg ttgagggttg ccaggatcag atcgcaggtg
     2101 acccggctgc cgtcgtcgag cccgctgccg tccaacagcg agatctgcga catgtcggtg
     2161 ccgagaccgc cgagccggtc gaccatggcg accaaccccg cagcggtcga tccctccccc
     2221 gacacctcgc ggccgatggc cttcaccagc agttccgcgg tgttgttgtc gctgaaggtc
     2281 agcatctgcg ccacgagttg ccatacggga cgcgaggcga tctccaacac ggtgttcgtc
     2341 gtctcgggag cctggccgct cgccggcgcg ccgccgacag cgatgccacg agcggtcagc
     2401 agttgggtga atgcgtcggc ggcccaggcg gcagggtctg aggcaggttg cgcgcccccg
     2461 ccagcacccg gaatcgggct gtaggttcgg gcatcgttta cgctgagcgc gctgagcggg
     2521 ccgacctgtc cctgggccag catccggctc ggccacgtcg acacggttcg ctcggcgtcg
     2581 aagaacgagt cgtccccgac gaccgacccg gtgatggcgg tgatcccgag cgccgcgagc
     2641 tcatcggcga tcgcctcgat actcgtgtgt gcccccttgc cccacttgaa cgtcgaccgg
     2701 tacgtgtcgg actccagcag cggatcgccg ccgccgatga tccagaggtc gccgttgatg
     2761 acgccaccgt cgatcggagc ggtcgtggtg aaacgggtcg tgaaggtgtg gtcggctccg
     2821 agcacctcga gcgcgaccca cgccgtgagc agcttctggt tcgatgccac ggcgagtgcc
     2881 tgatcggtgc cgtgggacac gatgacgcgc tcgccgtctc ggacctcgaa acagctcgtg
     2941 tcgctcaggg cagcgagctt cggagcgatg gcgttgttca gccgccggtc cgcttgggga
     3001 gcgacgagga acttggggac ccgtcgagcc gacagagccg cggtggtggc gctcggcgag
     3061 ttggcggcgg acgaggtcgc gggagtcgcc gcatcggagc gtgccgattc gtcactccac
     3121 tcccaccctc cgaccaggag tccggcgacg accatcccgg cgcccaccag cactgccgac
     3181 tggcttcggc gcggccgggc accgggtgag gcgtcgacgc gcccgactcg cccggtgggc
     3241 ccggcgtgct gcgaccgtcg tcgcgttggt tgcggcggtt gctgctgcgg ggcctgatgc
     3301 aagaagtcgt cggtcggcgg gcggtcggtc atggtcggcg ggtcacgcgt tctgttcgtg
     3361 gtggcggcgg aactcggcgt agcgggtcag gtgctcgagt gcggtgaccg cacgattggc
     3421 ggttgcgtaa cacacacggc ccgatgccct caccgccgcg ggaccggcgt tggtcggatc
     3481 ggagatcgcg agctcggtgc aacacaggat cggcttgccg gtggcctcgg agatctcggc
     3541 cgcggcgtgg gcgaagcggg cgtcttggcg gcggtggtac tcgacgatgc gctcgaggcc
     3601 gtgatccgga tagaacggtc cgtcgaccat catcgccgcc tggttcgatt ggatgccgag
     3661 tccgagatag acgatcgaat ccaccgaggg gtgccgggcg atgagttcca tcaccgtcgg
     3721 aatcgtgtcg cgggtttcgc cgccggcgag gtcgaccggg ttgtttcggc tccagcgcgg
     3781 cggaaggtgc tcatcgatcg ctgcgagcag atcgtcgggc aactcgacga gttcgagggt
     3841 gcggctggcg gaaatggcat cggcggtgac cactccccac ccacctgcgg tggtcatgac
     3901 caccacgcga ttgcccgctg gcaacggctg cgtggccagg gtggccgccg cttcgaaggc
     3961 ctcctcgacg gtgcgcgccc gggtgaggcc gccttgtcga catgcgccat cgaagacacg
     4021 atcgtcggtg gcgagcgagc cggtgtggct cgacgcggcc ttcgctcccc cggcggttgc
     4081 gccgcccttc acgacgacca ccggcattgt cggggtgatc tgtcgaacgg cgtcgaagaa
     4141 gccgcgacca tcctcgagcc cttcgacata ggcgagcccg accttggtcg ccgggtcgga
     4201 ctcgtagaac ccgaggaaat ccgccacgcc ggtggcggct gcgttgcctg ccgacacggc
     4261 ccggctgatg cccacgccgg tggccagcga caggttgaga aacgacgaga tgaagttccc
     4321 cgactgtgat gccacgccga tcgggccggc gggcgggtag ggcgcgacga tctgtgcgca
     4381 gagttgtgcc ggggtcgata ccaccccttg gccgttcggt ccggccagca ggattccgag
     4441 ttcctcgcac aacgcgacga gatcggcttc gtcgcggcgt ccctgttccc ccgcctcgcc
     4501 gtagccggcc gaggtcaaaa acgccgcctt gacgcccttc gccgcgcatt tgcgcaacag
     4561 gtccgggttg gcgtctttgg gggtgcagac gaagaccagg tcgatctcgc cggccggaag
     4621 ctcgtcgatg tcgggcaccg actgcacgcc gagcacctcg gccccgtctc gattcgtcgc
     4681 gaacaccttg ccggggaaac cgcacgccag cacgttgtgc agcgacacga acccgaactt
     4741 gccggggtgg gtcgaggctc cggcgatgag cacgccgcgc ggctcaaaga gcgccttgaa
     4801 ttgctcgtcg gtgggcatca gcgggttcct tcggtggtga gttcgaccaa cgcgtcgacc
     4861 gcgatcggcg tgccattggc gatgatgagc gggttcaggt cgatcgagtg caccgccggg
     4921 ttcgaggctg cggcatcgct caacgcgacc agcactgcgg ccagtgcctc ccggtcgacc
     4981 gctggttcgc cccggaactc accaagcaac gccaccgact cgagcgcatc gatcatctcg
     5041 tgggcatcgg ctgtggtgat gggcacgagc ccgacggcga catcgccgat cgcctcggcg
     5101 agcacaccgc cgatcccgac catgacggtc aacccgaact gcgggtcgcg ggccactccg
     5161 gcgatgagtt cccgggtgcc gctgatcatc gcagcgacga gcacgccgac ctctccgtcg
     5221 t
//
LOCUS       DAOOMA010000112         5213 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_19090_length_5213_cov_3.779178, whole genome shotgun sequence.
ACCESSION   DAOOMA010000112 DAOOMA010000000
VERSION     DAOOMA010000112.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 5213)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5213)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5213
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19090_length_5213_cov_3.779178"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..201)
                     /locus_tag="PLP95_04430"
     CDS             complement(<1..201)
                     /locus_tag="PLP95_04430"
                     /inference="COORDINATES: protein motif:HMM:NF024591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB45081.1"
                     /translation="MIISGGENVYCAEVENTLFAHPDIVDVAVYGRADDKWGEVPVAA
                     VVLAPGAQLTIDELEVWMDDKLA"
     gene            complement(202..1386)
                     /locus_tag="PLP95_04435"
                     /pseudo
     CDS             complement(202..1386)
                     /locus_tag="PLP95_04435"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
     gene            1607..3196
                     /locus_tag="PLP95_04440"
     CDS             1607..3196
                     /locus_tag="PLP95_04440"
                     /inference="COORDINATES: protein motif:HMM:NF024258.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HPB45082.1"
                     /translation="MGSEATPHSVRDDVSRSASARDPRWVKVAVVLFAAVVAILAMPM
                     TSSASREVGPGVVSARIAPSWPGETVLELPPLGRLAAATHRGPTQVALRLDEVDVRAA
                     QSLTTSTGAFDREHLNSLVEDDLPGLFWRVGLRLGVLSALVGAAAALLLPGRHRDRGW
                     LWRRTLMGSVAAVVAVSAVAASTAVGYSTDAFKQPRTSGPLSMVSPLIAQAGDNFTDG
                     LKQIDDRSKVLAQKLADLYSSTIDADVAASSGETVILHVSDIHLNPVGISLARELAST
                     FDVDAVIDTGDLTSFGQEPEARMVSELSKFDVPYYFVAGNHDGFEARKAIAAIDGVTA
                     IDGDVVTVGDVRILGVEDPTQTALRSIPRADLQRRYEAQYPRIEELLRRHEPDVLAIH
                     NPVQAVPAMGKVATVIGGHVHRFTLSESDGTVLATVGSSGATGLGALLVETRENYAFE
                     LLRFSGKRLVAIDTIELRGTRGEFVSRRHLIGPGVSYGDAAEVIDEMVIEPSADQFAT
                     TTTGSEPEPASESESTTTLQE"
     gene            3290..4327
                     /locus_tag="PLP95_04445"
     CDS             3290..4327
                     /locus_tag="PLP95_04445"
                     /inference="COORDINATES: protein motif:HMM:NF019346.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HPB45083.1"
                     /translation="MSEQFSSESHPHPQLHRDPTEIAAALVEALGRSVKGKSAQIGLL
                     VAGLLSGGHVLLEDLPGSGKTLLAKSLARATGGVVGRVQCTPELLPADIVGTSVWQPS
                     SSQWQFHPGPVFANVLLVDEINRASPRAQAALLEPMEERQVTVDGATYRLPDPFFCIA
                     TQNPHGQVGTYPLPESQLDRFSLVFSLGWPDRDAAREILVGAGGVSQLPAVVAVTNPT
                     ELANCIAEVRQVHVAASLVDYLLTLVDATRHHRSIDVGVSPRVAASLLALARAHAVVC
                     GRDFVTPEDFQTVFGPACAHRVRLGATDGASAAPAAAGIAGVPEIVAPSFGAAWNVLM
                     AVLASVPVPER"
     gene            4350..>5213
                     /locus_tag="PLP95_04450"
     CDS             4350..>5213
                     /locus_tag="PLP95_04450"
                     /inference="COORDINATES: protein motif:HMM:NF013998.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HPB45084.1"
                     /translation="MGNTARSWVRNAGDRSGTPGRVRPTAFGATLLVALVAAVVLRPG
                     VVDREAAAMVLVAVGALLVVGVAMPLVALRSLTVELEPLVTELDVAQVMRLRCTVFGS
                     AGVGPLRVRVASVGEPVPLALTAPSELQFPAPKRGRFETLEVEVVTEGPIGVVRLARR
                     FEVTLPRPLIVGPEVVAAQLPWQDLVVGTESSSSRQDEDGAVRAVRPYQFGDTISRVH
                     WRATARRGDVLVREYESEAHRRVTLAVDLGACDDPEAVASLAAGLALDGLRRGAHVVL
                     AVNDGRPHSTEV"
BASE COUNT          782 a         1623 c         1875 g          933 t
ORIGIN      
        1 ggcgagtttg tcgtccatcc agacctcgag ttcgtcgatg gtgagttgcg cgccgggggc
       61 caacacgacc gcggcgaccg gcacctcacc ccacttgtcg tcggcgcgcc cgtagacggc
      121 gacatcgacg atgtcgggat gggcgaacag cgtgttttcg acctcagcgc agtagacgtt
      181 ctcgccgccc gagatgatca tgtccttggc ccggtcgacg atccacacga acccctcctc
      241 gtcctggcga acgaggtcgc cggagtggaa ccacccgcct ctgaaggctt cggcggttgc
      301 gtcgtcgcgg cgccagtatc cctgcatgag gttcgggccg aggtagacga tctcacccac
      361 gtctccgacg gcgacgtcgt tcatctccgc atcgacgacc cggtacatca aggtgggaag
      421 cggcttgccg accgacccca gctttcgaag cgaatcttcg ccggacagtg cgcaggtgac
      481 ggggctcatc tcggtctggc caaacacggc gatgttggtg gcgttgggga agcactcggt
      541 catctggcgc aacaaggttt ccgacgcggg ggcggcgccc cagctgatga tgcgcagcgc
      601 cagatcggcc tctttgacgt aaggctcggc gacgatggcc tgccactggg tggggacgtt
      661 gaacacgacc gtggcgcgct cggcctccca ggccttgatc gtgtcgagcg cgttgaaagc
      721 gccgagggga tggatgatcg aagggatgcc gaggaggaac gaggtggcga tcgagccgag
      781 acctgcgatg tggaagaacg gggcggtcac gaaggcgacg tcatcggggt ggtcgccgtt
      841 gatgcgcacc atcgacgtgg cctggctgaa caggttgagg tgatcgagca tcgcaccctt
      901 gggccggccc gtggtcccgg aggtgtacat gatgagcgcc ggggtggact cggcgacatc
      961 gggggcctcg aagccgtcga gtggttcggc gatcagatcg ttcgaggact cgtggtcgct
     1021 gtcggggctg gcgtcgccga ggatgatgat ccgcttcagg cgcgacgtga tctgttgggt
     1081 ggcgccgatg ccctactggc cccgctcatc ggcgatgatc acgtccgcgt cggcgtcgtc
     1141 gatgatgaaa gcgatctcgg gtggggtcag gcgaatgttg atcggcaccg ccatcgcacc
     1201 gagggtgttg acggcaaaca ccgcttcgag cacctcggtg gagttcaggg tcaacaacat
     1261 gacgcggtcg ccgaagccga cgccgcgccg ctgaagggct gcggcgagtg attgcacgcg
     1321 atcgtggaac tcgctccagg tcgtcaccgc gccgaggtgc ttgaacgccg tcgcgtcggg
     1381 acgcattcgc gcatggactg cgacgtggtt catccagtgg ttgcgacgaa cggaccgtgg
     1441 aatgaccgag tcggacatgg gaccccctcc atgaacacta ttggcgatgc gccaacagta
     1501 gagggcggac gtgttgagag ccagcgatgt cctcggtcgg cggtggtcac cgccgtcggc
     1561 ggcgaccgca cgcggatgcg gtaggtcggg tggctacgct tcggccatgg gttccgaggc
     1621 gactccacac tcggtgcgag atgacgtctc gcgctcggcg tcggctcgcg acccacggtg
     1681 ggtcaaggtg gcggtggtgc tcttcgctgc ggtcgtcgcg atcttggcga tgcccatgac
     1741 ctcgtcggca tctcgcgagg tcggccccgg ggtcgtgagc gctcgcatcg caccgagttg
     1801 gccgggcgag acggtgttgg aacttccgcc gctcgggcgt ctcgcggctg cgactcaccg
     1861 tggtccgacg caagtcgcgc tgcgcctcga cgaggtcgat gttcgtgcgg ctcaatcgtt
     1921 gacgacctcg acgggggcgt tcgatcggga acatctgaat tcgttggtcg aggacgatct
     1981 tcccggactc ttctggcgtg tcgggctgcg gctcggggtg ctgtcggccc tcgtcggggc
     2041 agcggcggcc ctgctgttgc caggccgaca ccgagaccgg ggttggttgt ggcgccgcac
     2101 gttgatggga tcggtggccg cggtcgtggc ggtttcggcc gtcgctgcga gcacggcggt
     2161 gggctattcg accgacgcgt tcaaacaacc gaggaccagt ggtccgctgt cgatggtcag
     2221 cccgttgatc gcccaggctg gcgacaactt caccgacggt ctcaagcaga tcgacgatcg
     2281 ttcgaaggtg ctggcccaga agctcgccga cctgtactcc tcgacgatcg acgccgatgt
     2341 ggcggcctcg agcggcgaaa ccgtcatctt gcacgtgtcc gacattcacc tgaatccggt
     2401 gggaatctca ctggctcgcg agctggcgtc gaccttcgac gtcgacgccg tgatcgacac
     2461 cggcgacctg acgtcgttcg gccaggaacc cgaggcgcgc atggtgtcgg agctgtccaa
     2521 gttcgacgtc ccgtactact tcgtcgccgg caatcacgac gggttcgaag ctcgcaaggc
     2581 gattgcggca atcgacggag tgacggcaat cgacggcgac gtggtcaccg tcggcgacgt
     2641 tcggatcctc ggagtggagg acccgaccca gacggcgctt cggtccattc cccgagcgga
     2701 ccttcaacgc cgctacgaag cccagtaccc gagaatcgag gaactgttgc gtcgacacga
     2761 acccgacgtg ttggcgattc acaatccggt tcaggcggtt cccgcgatgg gcaaggtggc
     2821 gacggtcatc ggtggccacg ttcatcggtt cacgctgagt gaatcggacg gcaccgtgct
     2881 ggcgaccgtg gggtcgagcg gcgccaccgg gctcggcgcg ctcttggtgg aaacccgcga
     2941 gaactacgcg ttcgaactgt tgcggttctc cggcaaacgc ctggtggcca tcgacacgat
     3001 cgagttgcgt ggaacccgcg gcgagttcgt ctcgcgccgt catctcatcg gtccaggcgt
     3061 gtcctacggt gatgcggccg aggtgatcga cgagatggtg atcgagccgt ccgccgatca
     3121 gttcgccacc acgaccacgg ggtccgagcc cgagccagcg tccgaatccg agtcgacgac
     3181 gacactgcag gagtaatgcc gtctgaccga cggtgcgaca gtgaggtgtg agcgacccga
     3241 ggcgccttcg ccgtcgatcc ggcatcgccg cgtgtaaagc tgtgcggcga tgagcgaaca
     3301 gttctcgagc gagtcgcacc cgcacccgca gctgcaccgt gatccgaccg agatcgcggc
     3361 ggcgctcgtc gaggcgctcg gtcggtcggt gaagggcaag tcggcacaga tcggcctctt
     3421 ggtggcgggg ctgttgagcg ggggccacgt cctgctcgaa gacctccccg gctcgggaaa
     3481 gacgctgctg gccaaatcgc tggcccgcgc taccggtggc gtggtcgggc gtgtgcagtg
     3541 caccccggag cttctgccgg ccgacatcgt cggcacctcg gtgtggcagc cgagttcctc
     3601 gcaatggcag tttcatccgg gtccggtgtt cgcgaatgtg ctgctcgtcg acgagatcaa
     3661 ccgggcctcg ccgcgggcgc aggcagcact gctcgagccg atggaggagc gccaggtcac
     3721 cgtcgatggc gccacctatc ggttgcccga cccgttcttt tgcatcgcca cgcagaaccc
     3781 gcacggccag gtcggcacct atccgcttcc ggagagccaa ctcgacaggt tctcgttggt
     3841 gttctcgctc ggatggcccg accgcgacgc agcaagggag attctggtcg gggcgggagg
     3901 ggtcagtcag cttccggccg tcgtggcggt gaccaacccg accgagttgg cgaattgcat
     3961 cgcggaagtt cgtcaggtgc acgtggctgc gtcgttggtc gactacctct tgaccctggt
     4021 cgacgcaacc cgacaccatc gctcgatcga cgtgggcgtt tcgcctcgcg tcgcggcgag
     4081 ccttctggcg ctggcgcggg cccatgcggt ggtgtgcgga cgtgacttcg tgacccccga
     4141 ggacttccag acggtattcg gcccggcgtg cgcccaccgg gtgcggcttg gtgccaccga
     4201 cggcgcgtcg gctgcgcctg ctgcggcggg catagctggt gtgcccgaga tcgtcgcccc
     4261 gtccttcggc gctgcatgga acgtactcat ggcggtcctg gcctcggtgc cggtgcccga
     4321 gcggtaggga tcggcggcga gggtccggcg tggggaacac agcgaggtcc tgggtccgaa
     4381 acgccggcga ccgttcgggc acccccgggc gcgttcgacc gaccgcgttc ggtgcgacgt
     4441 tgctggtggc gctcgtcgcc gcggtggtgc ttcgaccggg cgtggtcgac cgggaggctg
     4501 cggccatggt gctcgtcgcg gtgggcgcgc tcctcgtggt cggggtcgca atgcccttgg
     4561 tcgcgctccg gtcgctcacg gtcgaactgg agccgctcgt caccgagctc gatgtcgctc
     4621 aggtcatgag attgcgctgc accgtgttcg ggtcggcggg ggtcgggccg ctgcgagttc
     4681 gcgtggcgtc ggttggcgag ccggtgccgc tcgcgttgac ggcgccgagc gagctgcagt
     4741 tcccggcgcc gaagcgcggg cgctttgaga cgctcgaggt tgaggtcgtc accgaaggcc
     4801 cgatcggggt ggtgcgattg gcgcgacgct tcgaggtgac cctgccacga cccttgatcg
     4861 tcggcccgga ggtggtggcg gcgcaattgc cgtggcagga cctcgtcgtc ggcaccgaat
     4921 cctcgtcgtc gcgacaggat gaggatggtg cggtgcgggc cgtgcggccc tatcagttcg
     4981 gcgacacgat cagcagggtg cactggcgtg cgaccgcccg ccgaggcgat gtcctcgttc
     5041 gagagtacga gtccgaggcc catcgccggg tgacgctggc ggtcgacctc ggagcttgcg
     5101 atgaccccga ggctgtggcg tcccttgcgg cggggctggc gctggacggg ttgcgacgag
     5161 gggcgcacgt cgtgctcgct gtcaacgacg gtcggccaca cagcaccgag gtt
//
LOCUS       DAOOMA010000113         5161 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_19350_length_5161_cov_4.044849, whole genome shotgun sequence.
ACCESSION   DAOOMA010000113 DAOOMA010000000
VERSION     DAOOMA010000113.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 5161)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5161)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5161
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19350_length_5161_cov_4.044849"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            138..353
                     /locus_tag="PLP95_04455"
     CDS             138..353
                     /locus_tag="PLP95_04455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB45085.1"
                     /translation="MTVPSQEQRLGVAVAEVVVARLDGDRGAAVAAADRLSQRSLARV
                     EVTYDALVAARRSADDAGADDAGAVGR"
     gene            350..1369
                     /gene="holA"
                     /locus_tag="PLP95_04460"
     CDS             350..1369
                     /gene="holA"
                     /locus_tag="PLP95_04460"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR01128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HPB45086.1"
                     /translation="MSEPIIVLKGSDAVLLGEAAQATLKALVGDRDADEVVDLFSGDD
                     YDVADAVMAATAVSMFGDRVIVMRHASRFNVEALGPLVAYASDPNPTSQLLVVWDKPL
                     ASGAHAHSVPKKLSDAVKACGGRVVDTGVAQTAKGRQSWLDERMAAASVSLTPAAKQV
                     VIERLGEDVSRLGALLRLLESTFPDGAKLEPRDVEPFVGEAGSVPPWELTDAIDSGDV
                     GGAVNKVRRMMHAGQRHPLQLMASLQAHFQRMLKLDGAAVRSEKDAADLLGMKGSTFP
                     AKKAMNQARSLGSARVSRAIVLLASADVDLRGRSALTPEATMEVLVARLAQLSRGSGT
                     ARGRR"
     gene            complement(1463..1735)
                     /gene="rpsT"
                     /locus_tag="PLP95_04465"
     CDS             complement(1463..1735)
                     /gene="rpsT"
                     /locus_tag="PLP95_04465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012230722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HPB45087.1"
                     /translation="MANIKSQIKRNKQNEKARLRNKAVRSNLKTRVKNAEAAAAAGAE
                     ETEATLRTAISTIDRAATKGVIHKNAAARKKSRLVKRIAVLQAAEG"
     gene            1917..3704
                     /gene="lepA"
                     /locus_tag="PLP95_04470"
     CDS             1917..3704
                     /gene="lepA"
                     /locus_tag="PLP95_04470"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006303521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HPB45088.1"
                     /translation="MTDLSRIRNLSIIAHIDHGKSTLADRILEICGAVDPRDMRAQYL
                     DSMDLERERGITIKLQSVKLDYDDHVIHLIDTPGHVDFGYEVSRSLAACEGVILVVDA
                     SQGIEAQTLANCYLALENDLEIVACLNKIDLPAADPDRYASELEQTLGIPADEILRIS
                     AKTGEGVAELLDAVIERIPAPVGNPDEPLQALIFDSHFDTYRGVVSSVRVVNGFLRTG
                     TRLKFMQSGSTHEAIEVGGRRPENTPVPALGPGEVGYLIAGIKDVGEARSGETITEFS
                     RPALEPLPGYMEPKPMVFCGLYPVDGDAFDDLRDALEKLRLNDSSFSYEPEVSGALGF
                     GFRVGYLGLLHMEIVRERLEREFGLNLIATAPSVEYRIVMNTGDEVVVDNPSDLPSPG
                     EFREIAEPIFACSILTPTDYVGTLMDLCQKRRGVMKKMEYISPERVELQYSIPLAEVV
                     IDFFDQLKSRTQGYASLDYEPAGYQAGQLVRVDILLNGEQVDAFCQIVHKDKAYDYGK
                     KMCEKLKEIIPRQQFDVPIQAAIGGRFISRQTVKAYRKDVTAKLYGGDISRKKKVLAK
                     QKEGKKRMKNIGRVELPSDAFITALRLDD"
     gene            3742..4743
                     /gene="hrcA"
                     /locus_tag="PLP95_04475"
     CDS             3742..4743
                     /gene="hrcA"
                     /locus_tag="PLP95_04475"
                     /inference="COORDINATES: protein motif:HMM:TIGR00331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HPB45089.1"
                     /translation="MLDDRKSAILRAVVQTYIETAQPVGSGHVVEAAAIGVSAATVRS
                     EMTVLEREGYLVQPHTSAGRIPTEKGYRFFVDEMGPARLGPEQTRTVSGFFAHVHGEL
                     EAMLQSTSRLLSRLTSTTAMVIGEVAETRKVRSVQLVALSERSYLTLVVLSAGTVLKH
                     LVERSTPVTADDLDEVGRLLGRALLDRPVTDVSTLDLAVPESLAALAAEASEAIRLAA
                     TESGRTLHIDGASNIAAAFDASDTLREVLSLLDQQMSVVTLIRDVLDRGLTVAIGSET
                     GVAPLAECSLVVAPFQVGGENAGAIGVLGPTRMNYQETLAAVAVVSNRLGHLLTEGE"
     gene            4754..>5161
                     /locus_tag="PLP95_04480"
     CDS             4754..>5161
                     /locus_tag="PLP95_04480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HPB45090.1"
                     /translation="MSADHYETLGVARTATAEEIKKAYRRLAREHHPDANQGDVAAET
                     RFKEIALAYEVLSDPDKRAHYDRFGDSSMGGMGGAGGDPFGGVGDIFDAFFGGGSPFG
                     GGRSRGPSGPPRGPEVEAVMTLDFVDAVFGGAHE"
BASE COUNT          893 a         1637 c         1742 g          889 t
ORIGIN      
        1 cgcgcaggac ccggacacgg tccgtgattc gttggcgctg cctccggacg ggttcgtcat
       61 cggtggcaga accgtggtgt tgagcgatga cggaacgctc ggattcgccc ccgatcccgg
      121 cggctaacgt tgcatcgatg acggtgccat cacaggaaca gcgcttgggg gtggccgtcg
      181 ccgaggtggt cgtggcccga ctcgacggcg atcgcggtgc agcggtggcc gcggccgacc
      241 gattgagtca gcggtcgctg gcccgggtcg aggtgaccta cgacgccctg gtcgccgcac
      301 ggcgctcggc cgatgatgct ggagccgatg atgccggagc cgtcggccga tgagcgaacc
      361 gatcatcgtg ttgaagggct ccgacgcggt gctcctcggc gaggcggcgc aggcgacgct
      421 caaggcgctc gtcggcgatc gcgacgccga cgaggtggtc gacctgttca gtggtgacga
      481 ctatgacgtc gccgatgcgg tgatggccgc cacagcggta tcgatgttcg gcgatcgcgt
      541 catcgtcatg cgccacgcca gccggttcaa cgtcgaggcg ctcggcccgc tcgtcgccta
      601 tgcctccgat cccaacccga cctcgcaact gctggtcgtg tgggacaagc cattggcgtc
      661 gggggcgcac gcccattcgg ttccgaagaa gctgagcgat gcggtcaagg cgtgtggggg
      721 gcgcgtcgtc gacacggggg tcgcgcaaac cgccaagggt cgccagagtt ggctcgacga
      781 gcggatggcg gcggcgtcgg tgagcctgac cccggccgcg aagcaggtgg tgatcgaacg
      841 gctcggcgag gacgtgtcgc ggctcggagc gctcctgcgg cttctcgagt cgaccttccc
      901 cgacggggcg aaactcgaac cccgcgacgt cgagccgttt gtcggtgaag cgggatcggt
      961 tccaccgtgg gaactcaccg atgccatcga ctcgggcgat gtcggtggtg cggtgaacaa
     1021 ggtgaggcgc atgatgcacg caggccagcg ccacccgcta caactcatgg catcgcttca
     1081 ggcgcatttc cagcgcatgc tcaagctcga cggtgcggcc gtgcgaagtg agaaagacgc
     1141 agccgacctg ctcgggatga agggctcgac cttcccggcc aagaaagcaa tgaaccaggc
     1201 tcgcagcctg ggaagtgcac gcgtcagccg cgccatcgtc ttgctcgcca gcgcagatgt
     1261 cgatctacgc ggccgtagcg cactcacccc ggaggcgacg atggaagtgc tggtcgcccg
     1321 cctcgcgcag ctgtctcgag gttcgggtac cgcgcgcggt cgccgttaac ccgcccgtct
     1381 caaccggttt tgtaggtact ttctgcggct ccagcaacag aaagtaccta caaaacgctg
     1441 gttgagggtc gctggtcgga gatcagccct cggcggcctg aagcacggcg atgcgcttga
     1501 ccaggcggga cttcttgcgg gccgcagcgt tcttgtgaat gacgcccttg gtggcggcgc
     1561 gatcgatcgt ggagatcgcg gtgcgcagcg ttgcctcggt ctcttccgca ccggcagcgg
     1621 cagcggcctc ggcgttcttg acgcgggtct tcagattgga gcggacggcc ttgttgcgca
     1681 ggcgggcctt ctcgttttgc ttgttgcgct tgatctggct cttgatgttg gccacggtga
     1741 accttgcagt ctcgggaatg aacgaacgtc gggtgtgcgc gcgagagcgg ctgcacagga
     1801 ctgaaagagc atatcggcca acgctagggc tcgtcacgtt ggcgtgcggc ccggccgcag
     1861 gttcttggta gcttcgaagg accgcccgct ggagggcggt tcgtgaaagt accgctgtga
     1921 ccgacctctc ccgaatccgc aacctctcga tcatcgccca catcgatcat gggaaatcca
     1981 ccctcgccga ccggattctg gagatctgcg gtgccgtcga tccacgcgac atgcgtgcgc
     2041 agtacctcga ctcgatggac ctcgaacgcg agcgtggcat caccatcaag ctgcaatcgg
     2101 tcaagctcga ttacgacgac cacgtcatcc acctgatcga cacccccggc cacgtcgatt
     2161 tcggctatga ggtgtcgcga tcgcttgcgg cgtgcgaggg cgtgatcctg gtggtcgacg
     2221 cgtcgcaggg catcgaggcg cagaccctcg ccaactgtta cctggcgctc gagaacgatc
     2281 tcgagatcgt ggcctgcctc aacaagatcg accttcccgc tgccgatccc gaccggtacg
     2341 caagcgagct cgaacagacc ctcggcatcc cggcggacga gattctgcgc atctccgcga
     2401 agacggggga gggggtggcg gagctgctcg acgcggtgat cgaacggatt ccggcgccgg
     2461 tcggcaatcc cgacgagccg ctgcaggcgc tgatcttcga cagtcacttc gacacctacc
     2521 ggggcgtggt cagctcggtg cgggtcgtga acgggtttct ccgcaccggg acgcggctga
     2581 aattcatgca gtccggttcg acccacgaag cgatcgaggt cggaggccga agacccgaaa
     2641 acacgccggt gccggcgctc gggccgggcg aggtcggcta tctcatcgcc ggcatcaagg
     2701 acgtcggcga ggcgcgttcg ggtgagacga tcaccgagtt ctcgcgcccg gcgctcgagc
     2761 cgctgccggg atacatggag cccaaaccga tggtgttctg cgggctttat ccggtcgacg
     2821 gtgacgcgtt cgacgacctg cgcgacgcgc ttgagaagct gcgtctcaac gacagctcgt
     2881 tctcctatga gcccgaggtg tcgggtgcgc tcggtttcgg gttccgggtc ggctacctcg
     2941 ggctgttgca catggagatc gtgcgagagc gcctcgagcg cgagttcggc ctcaacctga
     3001 tcgccaccgc accgtcggtc gaataccgga tcgtcatgaa caccggcgac gaggtcgtgg
     3061 tcgacaaccc gtcggatctg cccagcccgg gcgagttccg cgagatcgcc gagccgatct
     3121 tcgcgtgttc catcctcaca cccaccgact atgtcggcac gttgatggac ctgtgccaga
     3181 agcgccgtgg ggtgatgaag aagatggagt acatcagccc tgaacgagtc gagttgcagt
     3241 actcgatccc gctcgcggag gtggtcatcg actttttcga tcagctcaag agccgcaccc
     3301 agggctacgc ctcgctcgac tacgaacctg cggggtatca ggccggacaa ctcgttcgtg
     3361 tcgacatctt gttgaacggc gaacaggtcg acgcgttctg tcagatcgtg cacaaggaca
     3421 aggcgtacga ctacggcaag aagatgtgcg agaagctgaa ggagatcatt ccccgccagc
     3481 aattcgacgt accgatccag gccgcgatcg gcggacggtt catctcgcgt cagacggtca
     3541 aggcctaccg caaggacgtg accgccaagc tgtacggcgg cgacatcagc cgcaagaaga
     3601 aggtgttggc caagcagaag gaaggcaaga agcggatgaa gaacatcggg cgggtcgagt
     3661 tgccctccga tgcgttcatc accgcgttga gactcgatga ctgagcatct cattggcact
     3721 cgcgtagact gagtgccaat catgctcgac gatcgcaagt ccgccatcct ccgagccgtc
     3781 gtccagacct acatcgagac cgcccagccg gtgggctcgg gccatgtggt cgaggcggcg
     3841 gccatcggag tgtctgccgc caccgtgcgc agcgagatga cggtgctcga acgcgagggc
     3901 tacctggttc agccacacac cagcgccggg cgcattccca ccgagaaggg ctaccggttt
     3961 ttcgtcgacg agatgggtcc cgcccgactc ggacccgaac aaacccgaac cgtcagcggg
     4021 ttcttcgccc acgtgcacgg cgagctcgag gccatgttgc agtcgacctc acgactgctg
     4081 agtcggctga cctcgaccac ggcgatggtg atcggcgagg tcgccgagac ccgcaaggtg
     4141 cgctcggtgc aactcgtcgc cctcagcgag cggtcgtatc tgaccctggt ggtgttgtcc
     4201 gccggcacgg tgctcaaaca cctcgtcgag cgatcgactc cggtgaccgc cgacgacttg
     4261 gacgaggtgg gccgtctgct cggccgggca ttgctcgacc ggcccgtcac cgacgtgtcg
     4321 actctggatc tcgccgtccc cgagtcgctc gccgcactgg cggccgaggc cagcgaggcg
     4381 attcgcctgg cggccaccga gtcgggccga accctgcaca tcgacggagc gtcgaacatc
     4441 gccgcagcct tcgacgcgtc cgacacgctg cgagaagtgc tgtcgctgct cgatcagcaa
     4501 atgtcggtcg tgaccttgat ccgcgacgtg ctggatcgcg gtctgacggt ggcgatcggc
     4561 tccgaaaccg gggtcgctcc gttggcggag tgctcgctgg tggtggcccc gttccaggtc
     4621 ggcggcgaga acgccggggc catcggcgtg ctcggcccaa cgcgcatgaa ctaccaggag
     4681 acgctggccg ccgtggcggt cgtcagcaac cgactcggtc acctgctgac cgaaggggag
     4741 taacgagaca cccgtgagtg cagatcatta cgagaccctt ggcgtggcgc gcacggccac
     4801 cgccgaggag atcaagaagg cctatcgacg cctcgctcgg gagcatcacc ccgacgcgaa
     4861 ccagggcgac gttgcggccg aaacccgttt caaggagatc gcgctcgcgt acgaggtgct
     4921 gagcgatccc gacaaacgcg cccactacga ccgtttcggc gacagctcga tgggtggaat
     4981 gggcggtgct ggcggcgacc cgttcggcgg cgtcggagac atcttcgacg cgttcttcgg
     5041 cggaggctca ccgtttggcg gcggacgttc ccgagggccg tcggggccgc cgcgcggtcc
     5101 cgaagtcgag gcggtgatga ccctcgactt cgtcgacgcc gtgttcggcg gcgctcacga
     5161 g
//
LOCUS       DAOOMA010000114         5148 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_19425_length_5148_cov_3.835068, whole genome shotgun sequence.
ACCESSION   DAOOMA010000114 DAOOMA010000000
VERSION     DAOOMA010000114.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 5148)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5148)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5148
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19425_length_5148_cov_3.835068"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..164)
                     /locus_tag="PLP95_04485"
     CDS             complement(<1..164)
                     /locus_tag="PLP95_04485"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF014873.2"
                     /note="Produces ATP from ADP in the presence of a proton
                     gradient across the membrane. The beta chain is a
                     regulatory subunit; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HPB45091.1"
                     /translation="MTATETTLSDGRIVAIAGPVVDVEFPSDSIPQINDALEFTITVD
                     GVDVRVMAEVA"
     gene            complement(242..1225)
                     /locus_tag="PLP95_04490"
     CDS             complement(242..1225)
                     /locus_tag="PLP95_04490"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF004145.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HPB45092.1"
                     /translation="MPGAQERVLRQRIASTQNTKKITRAMELIAATRVVKAQMAANEA
                     RPYSEQITSVIANLAQGGVEVAHPLLRQSTDVRKVGVLVLSADRGLAGAYNSTVIRAA
                     ERVIQKAKDSGQSYSLVTVGRKAQAYFEFRNYRIEAAFEGMSDRPNYEFAREIAGKVT
                     GLFNDDVDVIELVYTRFISMGTQEAVVRRFLPLETSAADDAAGSGADDAGPSAQVEFE
                     PSPAEVLESLLPRYVESRIFAALLDSAASELASRQRAMKSATDNAQELIIKLQRKMNQ
                     ARQDAITTEISEIVGGANALNDDADDPEDLLLDHISSDPFPALTAKKHTNI"
     gene            complement(1229..2854)
                     /gene="atpA"
                     /locus_tag="PLP95_04495"
     CDS             complement(1229..2854)
                     /gene="atpA"
                     /locus_tag="PLP95_04495"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004723422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HPB45093.1"
                     /translation="MAELTINIADITAAIQKNLDGFNPDVSVGQVGRVIEVGDGIARV
                     SGLPDATVNEILEFESGKLGLALNLDEHSIGAVVLGEVDDIEEGQAVKATGEILSIPV
                     GDGMLGRVVNTLGQPIDGKGPLTNVVTRRVEVQAPGIMGRQPVGEPMQTGIKAIDSLI
                     PIGRGQRELIIGDRKTGKTTVAIDAIMNQKGLGVKCIYVAIGQKASTVAQTVARLEEA
                     GAMEYTVVVVAPASDPAPFKYLAPYAGCAMGQHWMVNGDHGLIIYDDLSKQAEAYRQV
                     SLLLRRPPGREAYPGDVFYLHSRLLERAAKLSDELGAGSLTALPVIETKAGDVSAYIP
                     TNVISITDGQVFLQDDLFKSGVRPAIDVGISVSRVGSAAQIKAMKTAVGTLKSDLQQF
                     RELEAFAAFGSDLDAVSKAQLERGYRLTELLKQGVGEPVPVQEQVVILYAGTRGFLDN
                     VDIADVRRFEAELVEWFRTRHGDLLAAIKETGKVDEETLDAGVKAFATQFRATQAAAA
                     VEATAEGDLASTGTAHGSNHLPEGESVLADDEA"
     gene            complement(2913..3440)
                     /gene="atpH"
                     /locus_tag="PLP95_04500"
     CDS             complement(2913..3440)
                     /gene="atpH"
                     /locus_tag="PLP95_04500"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF012439.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit delta"
                     /protein_id="HPB45094.1"
                     /translation="MSEAVNNAYAEAIGAVARAEGQASTVERELVAVASAITGSDELR
                     TTLGDTKIPAARRIQIVEDLLGGNAAPATVSIVSLIVANGRVGDLEAIAKAVLNRGAA
                     ARGEEVAEVRSAVALNDEQIERLAAALKAKLHRDVTIRNIVDPSVLGGIITQVGETLL
                     DGSVRTRMTQLREAF"
     gene            complement(3455..3976)
                     /gene="atpF"
                     /locus_tag="PLP95_04505"
     CDS             complement(3455..3976)
                     /gene="atpF"
                     /locus_tag="PLP95_04505"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HPB45095.1"
                     /translation="MIAFLAAGNGNWLPHDINEVYWGTAAFLIVFGLIIWKAGPAIKT
                     AIAARPARIAEELGSAATARASGEAKVAEVKNALADSDKEAARIIDEARQTASRLSSD
                     TVARAEAEAVAIRERGAADLVAARRQAQADLTNEVSRLALGAAERVVDANLTDDTQQA
                     LIDSYISQVGATN"
     gene            complement(3987..4532)
                     /gene="atpF"
                     /locus_tag="PLP95_04510"
     CDS             complement(3987..4532)
                     /gene="atpF"
                     /locus_tag="PLP95_04510"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HPB45096.1"
                     /translation="MLAVALSLLSEGGEAAEKVANPVLPTVPEMVWGAIAFFSLLALM
                     KFVLLPPIKQSTRKREERIRADEEAAERAIVESEQIRRDYDATLAEARAEAARIIDEA
                     RESAEAKRSEIIRAAEDEVANARQGALAELETERGSALDSLRTQVATIAVSAAGKVIQ
                     KPLDVAANQAVVDAHVSRADA"
     gene            complement(4673..4888)
                     /gene="atpE"
                     /locus_tag="PLP95_04515"
     CDS             complement(4673..4888)
                     /gene="atpE"
                     /locus_tag="PLP95_04515"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015799191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HPB45097.1"
                     /translation="MTAAGGAGIAYGLAAIGPAIGIGYLVGQSVQAIARQPEAAGQVQ
                     TTMFLGIAFTEALALIGFVVFILLKFV"
BASE COUNT          837 a         1595 c         1767 g          949 t
ORIGIN      
        1 gcgacctcgg ccatgacccg cacgtcgacg ccgtccaccg tgatggtgaa ctcgagcgcg
       61 tcgttgatct gcgggatcga gtcggatggg aactccacgt cgaccaccgg gccggcaatg
      121 gcgacgatac ggccgtcgga gagcgtggtt tcggttgcgg tcatggcgtc gtttcctcgt
      181 tggtcttcgg gagatggtcg tgacgggagt ggcaacccga gaatccgggg cgccgaatcg
      241 ttcagatgtt ggtgtgtttc ttggcggtca gcgccgggaa cggatcggag ctgatgtggt
      301 cgagcagcag atcctcggga tcgtctgcat cgtcgttgag tgcgttcgca ccgccgacga
      361 tctcggagat ctcggtggtg atggcatcct ggcgagcctg gttcatcttg cgctgaagct
      421 tgatgatgag ctcctgtgcg ttgtccgttg ccgacttcat cgctcgctgg cgactcgcca
      481 gttccgacgc cgccgagtcg agcaacgccg cgaaaatgcg gctttcgacg tagcggggaa
      541 gcagcgactc gagcacctcg gccggcgagg gttcgaattc gacctgggcg ctcgggccgg
      601 catcgtcggc gcctgaaccc gctgcgtcat ctgcggcgga ggtctcgagc gggaggaaac
      661 gacgaacgac cgcctcctgg gtgcccatcg agatgaacct cgtgtacacc agctcgatca
      721 catcgacatc gtcgttgaac aacccggtca ccttgccggc gatttcccgg gcgaattcgt
      781 agtttggacg atccgacatg ccctcgaagg ccgcttcgat gcgatagttg cggaactcga
      841 aatacgcctg cgccttacgc ccgaccgtga cgagcgaata gctctgaccg gagtctttgg
      901 ccttttggat cacacgctcc gcggcccgga tgaccgtcga gttgtaggcg ccggcgaggc
      961 cgcgatcggc gctgagcacc agcaccccca ccttgcgcac gtcggtcgac tgtcgcaaca
     1021 gcgggtgggc cacctcaact ccgccctgag cgagattggc gatcaccgag gtgatctgct
     1081 cgctgtaggg gcgagcctcg ttggccgcca tctgggcctt cacgacgcgg gtggcagcga
     1141 tcagctccat ggcgcgggtg atcttcttgg tgttctgggt gctggcgatt cgctggcgga
     1201 gtacccgctc ttgtgcacct ggcatggatc aggcctcgtc gtcggcgagg accgactcgc
     1261 cctcggggag gtggttggaa ccgtgggcgg tgccggtgga ggccaagtcg ccctcggcgg
     1321 tcgcttccac cgctgctgcg gcctgcgtcg cccggaactg cgttgcgaac gccttcacgc
     1381 cggcgtcgag ggtctcctcg tcgaccttgc cggtctcctt gatcgcagcg agcaggtcgc
     1441 cgtgacgggt acggaaccac tcgacgagct cggcttcgaa ccggcgaaca tcggcgatat
     1501 cgacgttgtc gaggaatccg cgggtgccgg cgtagaggat gaccacctgc tcctgaaccg
     1561 gaaccggttc acccacgccc tgcttgagca actcggtgag acgataccca cgctcgagct
     1621 gcgccttcga cacggcatcg aggtcagaac cgaaggcggc gaacgcctcg agctcgcgga
     1681 actgctgcag atcggacttc agcgtgccga ccgcggtctt catcgccttg atctgtgcgg
     1741 ccgaacccac gcgtgacacc gagatgccca cgtcgatcgc ggggcgtacg cccgacttga
     1801 acaggtcgtc ctgcaggaag acctggccgt cggtgatcga gatcacgttc gtcggaatgt
     1861 aggcggacac gtcgccggcc ttggtctcaa tgaccggaag cgcggtgagc gaaccggcac
     1921 ccagctcgtc agagagcttg gcggcacgct caagcagacg gctgtgcagg tagaacacgt
     1981 cgcccggata ggcctcgcgg cccggcggac ggcgcagcaa cagcgacacc tgacggtagg
     2041 cctcggcctg cttggagaga tcgtcgtaga tgatgaggcc gtgatcgccg ttcaccatcc
     2101 agtgctgacc catggcgcaa ccggcatagg gagcgaggta cttgaacgga gccgggtccg
     2161 aggccggagc caccacgacg accgtgtatt ccatcgcacc ggcctcttcg aggcgggcca
     2221 cggtctgcgc gacggtcgag gccttctgcc cgatcgccac gtagatgcac ttcacgccca
     2281 gacccttctg gttcatgatg gcgtcgatcg cgacggtggt cttacccgtc ttacggtcac
     2341 cgatgatcag ctcacgctgc ccacgtccga tcgggatcag cgagtcgatc gccttgattc
     2401 ccgtctgcat gggttcaccc acgggctggc ggcccatgat tccgggcgcc tgcacctcga
     2461 cgcgtcgggt gacgacgttg gtgagcgggc ccttgccgtc gatcggctgg ccgagggtgt
     2521 tgaccactcg gcccaacatt ccatcgccca ccggaatcga caggatctcg cccgtcgcct
     2581 tcacggcttg tccctcttcg atgtcgtcga cctcgccgag gaccacggcg ccgatcgagt
     2641 gctcgtcaag gttgagcgcg aggccgagct tgcccgactc gaattcgaga atctcgttga
     2701 cggtcgcgtc cggaaggccc gacacgcggg cgataccgtc gccgacctcg atgacacggc
     2761 caacctggcc gacgctcacg tcggggttga acccatcgag gttcttttgg atggctgccg
     2821 tgatgtcggc gatgttgatg gtcaactcgg ccatggtgtg tcgttgctcc tgagcaggtg
     2881 aaaggtgcgg gctggcgggc gacgccgagg cgtcagaagg cctcgcgcag ctgggtcatg
     2941 cgggtacgga cagaaccgtc gagcagcgtc tcaccgacct gggtgatgat gccgccgagg
     3001 acggacgggt cgacgatgtt gcggatggtg acgtcgcgat ggagcttcgc cttgagcgcg
     3061 gcggcgagcc gctcgatctg ctcatcgttc aacgcgaccg cggaacgaac ctcggcgacc
     3121 tcttcgccgc gggcagccgc tccacggttg agcacggcct tggcgatcgc ttcgagatcg
     3181 ccgacgcggc cgttcgcaac gatgagcgac acgatcgaca ccgtcgccgg cgccgcattg
     3241 ccgccgagga gatcctcgac gatctgaatg cgacgggccg cggggatctt ggtgtcgcca
     3301 agggtggtgc gcaactcgtc ggagccggtg atcgcggagg cgacggcgac gagctcgcgc
     3361 tcgacggtgc tggcctgacc ctcggcacgt gcgacggccc cgatggcctc tgcgtaggcg
     3421 ttgtttacag cttcggacat ggtgattcct cgggtcagtt ggtcgcgccg acctggctga
     3481 tgtagctgtc gatgagcgcc tgttgggtgt cgtcggtgag gttcgcgtcg acgacccgtt
     3541 cggctgctcc gagtgcgagg cggctcacct cgttggtgag atcggcttgg gcctgacggc
     3601 gtgccgcgac caggtcggcg gcgccgcgct ctcggatggc gaccgcctcg gcctcggcgc
     3661 gggccacggt gtcggaactg agccgtgacg cggtctggcg tgcctcatcg atgatgcggg
     3721 cggcttcctt gtcggaatcg gccagggcgt tcttcacctc ggccaccttg gcctcaccgc
     3781 tcgcacgagc cgtcgcggcg gaaccgagtt cctcggcgat gcgcgccggc cgagcagcga
     3841 tagcggtctt gatggcggga cccgccttcc agatgatgag cccgaacacg atgagaaacg
     3901 ctgcggttcc ccagtagacc tcgttgatgt cgtgtggcaa ccagttgccg ttgcctgcgg
     3961 cgagaaatgc gatcatctcg tactccttag gcgtcagcgc ggctgacgtg ggcgtcgacg
     4021 acggcctggt tggcggcgac gtcgaggggc ttttggatga ccttgccggc ggccgaaacg
     4081 gcgatcgtgg cgacctgggt acgcagcgaa tcgagcgccg agccacgctc ggtctcgagt
     4141 tcggcgagcg caccctgtcg ggcgttagcg acctcgtcct cggcagctcg gatgatctcg
     4201 gaacgcttgg cctcggcgga ctcgcgtgcc tcgtcgatga tacgagcggc ttcggcacgg
     4261 gcctcggcga gcgtcgcgtc gtaatcgcgg cggatctgtt cggactcgac gatggcgcgc
     4321 tcggcggctt cctcatcggc acgaatgcgc tcttcgcgct tgcgcgtcga ctgcttgatc
     4381 ggcggcagaa gcacaaactt catgagggcg agcagcgaga agaatgcgat cgcaccccag
     4441 accatctcag ggacggtcgg aagaacgggg ttggctacct tttcggcggc ttcaccaccc
     4501 tcgctgagga ggctgagcgc tacggcaagc atgggttctc ctgttgaaga aatgacggac
     4561 aaagtttcgg ttggcgactc gtggtggtgg gggtgcaaca cccccacccc actaccaaca
     4621 actcacagtt cgcgatgcgc ggcggtcggg ccacctcgca tccgtgctga gatcagacga
     4681 acttgagaag aatgaagacc acgaagccga tcagcgcgag ggcttcggtg aaggcgatgc
     4741 cgaggaacat cgtggtctgc acctggccgg cggcctcggg ctggcgagcg atcgcctgga
     4801 ccgactgacc gacgaggtag ccgatgccga tggccgggcc gatggcggca agaccgtacg
     4861 cgatgccggc gccgccggct gcggtgatgc ccttgacatc ggcggcggtc agttcctcag
     4921 agttgaactg cgacgtcgcg tcgatggcgt gctgggcgat ggagctgagc gtggacatgg
     4981 ttgttggttt ctcctgtgag ttccggtgcc gtgcaccaga ggttggtcag gtctcggtgg
     5041 ggagcaacgc tcccccacga atttgttgag tgaagcagtg cgccgggtgc agggcgccac
     5101 cgatgtacac accggtgagc agggcgaaga tgtaggccag aaggaagg
//
LOCUS       DAOOMA010000115         5143 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_19453_length_5143_cov_3.962068, whole genome shotgun sequence.
ACCESSION   DAOOMA010000115 DAOOMA010000000
VERSION     DAOOMA010000115.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 5143)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5143)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5143
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19453_length_5143_cov_3.962068"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1010)
                     /locus_tag="PLP95_04520"
     CDS             complement(<1..1010)
                     /locus_tag="PLP95_04520"
                     /inference="COORDINATES: protein motif:HMM:NF017536.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HPB45098.1"
                     /translation="MAHEQNSTTGGSTTTDPILQVVELAQQWPTIDPFLFTAHHLDDF
                     PAGDDRLAPVESTAGRPIGSDFTGLNGWSMYHGSAVPGFPQHPHRGFETVTYVRTGHI
                     DHHDSMGAAARFGKGDTQWMTAGRGVVHSEMFPLIEQDRRNPTELFQIWLNLPADDKM
                     VDPYFTMFWGEDTPKVSIVNEHGASATVTVVAGPFGDATPKAPPPNSWAARPEADVAI
                     WHLVLDEHASLDLPPAQSDETVRTLYVFHGAGARIAGRDIEASRGVLVDASAPLKIEA
                     ATGPVEILVLQGRPIGEPVARYGPFVMNTKAQIQQAFDDYQRTQFGGWPWPTDDPAHP
                     RD"
     gene            1253..2332
                     /locus_tag="PLP95_04525"
     CDS             1253..2332
                     /locus_tag="PLP95_04525"
                     /inference="COORDINATES: protein motif:HMM:NF013219.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HPB45099.1"
                     /translation="MSSTASKSDLASATSARVSVTAVFGVLIAIVVLGTIYSAGRGQL
                     PISPGEVVGSILRKVGISGGAEMTHPNGDVTLWNVRFPRIAMALLVGAALAVAGAVMQ
                     GIFGNPLAEPSVIGVSSGAAVGACAAIVWKVAWFGAYTTPVFAFVTSLITTFAVYAMS
                     RRDGKAEVVTIVLMGIAVNALASGLVGWFVFMGDTAAREQIVFWQLGSLNGSRWQQVW
                     AVAPLIGIGLIGSVLIARQLDLLALGERSARHLGVNVERLRLIAIVLSSLLVGPAVAF
                     SGIIGFVGLIVAHLIRMLIGPAHRRLIPAAALGGASVLLIADVIARTAKDYADLPIGM
                     LTAVVGGPTFFFLIRKTRKHAGGWA"
     gene            2329..3180
                     /locus_tag="PLP95_04530"
     CDS             2329..3180
                     /locus_tag="PLP95_04530"
                     /inference="COORDINATES: protein motif:HMM:NF010068.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter ATP-binding protein"
                     /protein_id="HPB45100.1"
                     /translation="MTRMIFGATPRVPSPVELGDVVLSAKDLSVTIAGNTILDKVSLE
                     VRSGEIVALVGPNGAGKSTLLAAITGDRDCDSGTVEIGNAPASEWTQLELAMRRSVLL
                     QRIEMSFPFAVAEVIRMGREPWRNTDLEDEDDEAMAWAVEATDVGHLLDRSFTTLSGG
                     EQARAALARTLSQRTALLMLDEPTAALDIAHQEQVLRVARERARLGDAVVVVLHDLAL
                     AASYSDRVVILSEGRVVASGSPIDVMTDELLSSVYGCRIETMAHPRTGALLIFPVRDD
                     VDSFAQG"
     gene            3202..4440
                     /locus_tag="PLP95_04535"
     CDS             3202..4440
                     /locus_tag="PLP95_04535"
                     /inference="COORDINATES: protein motif:HMM:NF015451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03862 family flavoprotein"
                     /protein_id="HPB45101.1"
                     /translation="MSFSAIVVGGGPGGLMAAEHLAAAGGSVRLFERTPSVGRKLLLA
                     GRSGLNLTHSEPTDELLARYGAATGRLSEAIRRFDADALREWARGLGETTFVGSSGRV
                     FPEAMRANGLLKAWIARLDSLGVEIVVGHRWIGWGDDGVLRFETSDGDVVEAGADIVV
                     LALGGASWPSVGSDGRWVEAVSEAGIAVAPLRAANSGFEVAWTPQFLNKHEGEPIKNV
                     AVTCGDVSSRGEAVITESGVEGGVIYAVGAVLRDVAEANGSATAMMNLHPDLSRERLI
                     ERLARRRAKDTLSTTLKRSGLEPVKAALVNEVLRAPGQLGGRDAPGASDVEALADLVM
                     ALPLRVLGPTPIDRAISTAGGISFDELDERFMIRRFPGTFAVGEMLDWEAPTGGYLLQ
                     ATFATAVAAANGALDWLSER"
     gene            complement(4502..>5143)
                     /locus_tag="PLP95_04540"
     CDS             complement(4502..>5143)
                     /locus_tag="PLP95_04540"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HPB45102.1"
                     /translation="ADQLAYAAALPKEAEPGTVFNYSSGVSLLLGEVIAQSTGMSADD
                     YAREKLFDPMQFSKADWWQDAAGHTLTYCCVDTTSRDFARFGWLYLNDGNWDGTQLVP
                     ASWVEESLTGAPTSPDQYGYQWWLEDYPGVPDDMFMARGFDGQYIFAIPSLDLVVVRN
                     GTYVKDPGPAVADPNLFAVYPPNGFIPGKGTIAPSDGWDTAGFIAGVVAAIHD"
BASE COUNT          771 a         1550 c         1860 g          962 t
ORIGIN      
        1 tcgtcacggg gatgcgccgg gtcgtcggtc ggccacggcc accctccgaa ttgagtgcgt
       61 tggtagtcgt cgaaggcctg ttgtatctgc gccttggtgt tcatcacgaa cggcccgtag
      121 cgcgccaccg gttcgccgat cgggcggccc tgcaggacga gaatctcgac cgggcccgtc
      181 gccgcttcga tcttgagcgg cgcgctggca tcgacgagga cgccgcggga tgcctcgatg
      241 tcgcgcccgg cgatccgcgc ccccgccccg tgaaagacat acagggtgcg aacggtctca
      301 tcgctttgcg ccggcgggag atcgagcgac gcgtgctcgt cgagcacgag gtgccaaatg
      361 gcgacatcgg cctcggggcg agcggcccac gagttgggcg gtggggcctt cggcgtcgca
      421 tcaccgaacg gtccggcgac gacggtcacc gtcgcgctcg cgccgtgttc gttcacgatc
      481 gacaccttgg gcgtgtcctc tccccagaac atggtgaagt aggggtcgac catcttgtcg
      541 tccgctggga ggttcaacca gatctggaac agttcggtcg ggttgcgtcg gtcctgttcg
      601 atcagcggaa acatctcgct gtgcacgacg ccgcgaccgg cggtcatcca ctgtgtgtcg
      661 cccttgccga acctcgctgc cgcgcccatc gagtcgtgat ggtcgatgtg tccggtgcgt
      721 acgtaggtga ctgtttcaaa tccgcggtgc gggtgttgcg ggaaccccgg aaccgcgctg
      781 ccgtggtaca tcgaccagcc gttgagaccg gtgaaatcgg agccgatcgg tcgcccggca
      841 gtcgactcga cgggagcgag gcggtcgtca cccgcgggga agtcgtcgag gtggtgagcg
      901 gtgaagagga acgggtcgat cgtgggccac tgctgtgcaa gttcgaccac ttggagaatc
      961 ggatcggtcg tcgttgagcc gccggtggtg gaattctgct cgtgtgccat gccataccca
     1021 acagctccga gacccccggt attccggcgg tccggggcct gcgggccggg tcgggccgac
     1081 cgtcgagacg gccgagtgtg gcaccacagc cagcttcgcc tgagtttgtg acgggcgcgg
     1141 cgacgtgtag tgttaggccc gccttattcg ggccggaccg acctgcagcg gacgggcgcc
     1201 gaggcgacaa accttccgaa gtcccgaacc cgccgaggag cgagtcaccg cgatgtcgtc
     1261 cactgcgtcg aaatccgatc tggcgtcggc cacctccgct cgtgtgagcg tcaccgcggt
     1321 gttcggagtg ctgatcgcca tcgtggttct cggcacgatc tactcggccg ggcgcggtca
     1381 gttgccgatc agccccggcg aggtggtcgg atcgattctg cgcaaggtcg ggatcagcgg
     1441 cggtgcggag atgacccacc ccaacggtga cgtgacgctg tggaacgttc gctttccacg
     1501 aattgcgatg gcgttgctgg tgggcgccgc cctcgcggtg gcgggtgcgg tgatgcaggg
     1561 aatcttcggc aatccgctcg ctgaaccatc ggtgatcggc gtgtcgtcag gggcggcggt
     1621 cggagcctgt gcggccatcg tgtggaaggt cgcatggttc ggtgcgtaca cgacgccggt
     1681 cttcgcgttc gtcacgagcc tcatcacgac gttcgcggtc tacgccatgt cgcgacgcga
     1741 tgggaaggct gaggtggtca cgatcgtgtt gatggggatc gccgtcaacg ccttggcctc
     1801 gggtctggtg ggctggtttg tgttcatggg cgataccgcg gcccgcgagc agatcgtgtt
     1861 ctggcaactc ggaagcctca acggcagcag gtggcaacag gtgtgggcgg tcgcaccgct
     1921 gatcggcatc ggattgatcg gctcggtgct catcgcccgt caactcgacc tgctcgcgct
     1981 cggtgaacgg tcggcccgtc acctcggggt caacgtcgaa cggcttcgcc tcatcgccat
     2041 cgtgttgtcc tcgctgctcg tcggtccggc ggtggcgttc tcgggcatca tcgggttcgt
     2101 cgggctgatc gtggctcacc tgatccgcat gctcatcggg ccggcgcatc gacgtctgat
     2161 ccccgcggcc gcccttggtg gtgccagcgt gttgttgatc gccgacgtga tcgcccgaac
     2221 cgccaaggac tacgcagatc tccccatcgg catgctcact gcggtggttg gcggcccgac
     2281 gttcttcttc ctcatccgca agacacgcaa gcacgcgggc ggctgggcat gacgcgcatg
     2341 attttcggcg ccacgccgag ggtcccgtcg ccggtcgaac tcggcgatgt cgtgttgtca
     2401 gcgaaggacc tcagcgtcac gatcgcgggc aacacgatcc tcgacaaggt gtccctcgag
     2461 gtccgatccg gtgagatcgt cgcgctcgtg gggcccaacg gggccggcaa gtcgacgctg
     2521 ctcgcagcga tcaccggtga tcgcgactgc gacagcggca cggtcgagat cggcaacgcg
     2581 ccggcatcgg agtggaccca actcgagttg gccatgcgtc gcagcgtgct gttgcagcgc
     2641 atcgagatgt cgtttccgtt cgcggtcgcc gaggtcattc ggatgggacg cgagccgtgg
     2701 cgcaacaccg accttgaaga cgaggacgac gaggcgatgg cttgggcggt cgaggcgacc
     2761 gacgtcggcc acctgctcga tcggtcgttc acaacgttgt cgggtggcga gcaggcgaga
     2821 gctgcgctgg ccagaacact ttcgcagcgc acagcgctgt tgatgctcga cgagccgacc
     2881 gctgcgctcg acatcgccca ccaggaacag gtgttgcggg tcgcccgtga gcgggcccga
     2941 ctcggcgatg cggtggtggt ggtgctgcat gatctggcgc ttgccgcgtc gtactcggac
     3001 cgtgtggtga tcttgtccga gggacgcgtg gtggccagcg gatcgccgat cgacgtgatg
     3061 accgatgagt tgttgagctc ggtgtatggc tgtcgtatcg aaaccatggc tcatccccgc
     3121 accggcgcac tgttgatctt cccggtgcgc gacgacgtcg attcgttcgc tcagggctga
     3181 gggcgcggca acactgttcc gatgtcgttc agtgcgatcg tcgtcggcgg aggaccgggt
     3241 gggctcatgg ccgccgaaca cctggcagcc gccggcggat cggtgcggct gttcgagcgg
     3301 acgccgtcgg tcggccgcaa gctgttgctc gccggtcgat cagggctcaa cctgacccat
     3361 tcggaaccga ccgacgagtt gcttgcccgt tacggagcgg caaccggccg gctgtcggaa
     3421 gcgattcgtc gtttcgacgc cgacgcgttg cgtgagtggg cgcgaggcct gggggagacg
     3481 acctttgtgg ggtcgagcgg ccgtgtgttt cccgaggcga tgcgagccaa cggattgctg
     3541 aaggcgtgga tcgcccgact cgattccctc ggtgtggaga tcgtcgtcgg tcatcgttgg
     3601 atcggctggg gcgacgatgg ggtgttgcgc ttcgagacaa gcgacggtga cgtggtcgag
     3661 gcgggcgcgg acatcgtggt gttggcgctc ggcggtgcga gttggccgag cgtgggttcg
     3721 gatggccggt gggtcgaagc ggtcagcgag gccggcatcg cagtcgcgcc actacgtgca
     3781 gcgaacagcg gcttcgaggt ggcatggact cctcagtttc tgaacaagca cgaaggcgag
     3841 cccatcaaga acgtcgccgt gacctgcggt gatgtgtcgt cccgagggga ggcggtcatc
     3901 accgagtccg gggtcgaagg cggggtgatc tatgcggtcg gagcggtgct gcgcgacgtg
     3961 gcggaggcga acggttcggc gaccgcaatg atgaacttgc accccgacct gtcgcgtgag
     4021 cgtctcatcg agcggttggc gcggcggcga gccaaggaca cgctgagcac gacgctgaag
     4081 cgcagtggcc tcgagccggt gaaggcggcg ctggtgaacg aggtgctgcg tgctcccggg
     4141 cagctcggtg ggagagatgc tccgggggcg agtgatgtcg aggcacttgc ggacctggtg
     4201 atggcattgc cgttgcgggt gctgggcccg acgccgatcg accgtgcgat cagcaccgcc
     4261 ggcggcatct cgttcgacga gctcgacgag cgtttcatga ttcgtcggtt ccccggaacc
     4321 ttcgctgtcg gcgagatgct cgattgggag gccccgaccg ggggctacct cttgcaggcg
     4381 acgttcgcga cggcggtcgc cgcggcgaac ggtgcgctcg actggctgag cgaacgctga
     4441 gcgagcgctg agcaggtggt gttgtcgacg cgcccttggt cggttcagtc gtgggccgcg
     4501 ttcagtcgtg gatcgcggcg accacgccgg cgatgaaccc ggcggtgtcc catccgtcgc
     4561 ttggagcgat ggtgcccttg ccagggatga acccgttcgg tgggtacacg gcgaacaggt
     4621 tcggatccgc cacggcgggg cccggatcct tcacgtaggt cccgttgcgg acgacgacga
     4681 ggtcgaggct tgggatagcg aagatgtact ggccgtcgaa accgcgggcc atgaacatgt
     4741 cgtcgggaac tcccgggtag tcctcgagcc accattggta cccgtactga tccggagacg
     4801 tcggggcgcc ggtgagcgat tcctccaccc agcttgccgg tacgagttgg gttccgtccc
     4861 agttgccgtc gttgagatag agccagccga acctggcgaa gtcgcggctc gtggtgtcga
     4921 cgcagcagta ggtgagcgtg tggccggcgg cgtcctgcca ccagtcggct ttggagaact
     4981 gcatcgggtc gaacagcttc tcgcgggcgt agtcgtccgc cgacatgccg gtcgattggg
     5041 cgatcacctc gccgaggagg agcgacacgc cgctcgaata gttgaacacc gtgcccggtt
     5101 cggcttcctt cggtagtgct gcggcatacg cgagctggtc ggc
//
LOCUS       DAOOMA010000116         5117 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_19601_length_5117_cov_5.546424, whole genome shotgun sequence.
ACCESSION   DAOOMA010000116 DAOOMA010000000
VERSION     DAOOMA010000116.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 5117)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5117)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5117
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19601_length_5117_cov_5.546424"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..570)
                     /locus_tag="PLP95_04545"
     CDS             complement(<1..570)
                     /locus_tag="PLP95_04545"
                     /inference="COORDINATES: protein motif:HMM:NF014518.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar cap protein FliD N-terminal
                     domain-containing protein"
                     /protein_id="HPB45103.1"
                     /translation="MAIDGLASGLDTAKIITSLMAIERIPVNQMEARQAKAQSAIDAY
                     NSILTKMTAVTTAANNLDRANDWSLSATTSSNIALATAQVSSGATTAAGSLTFTVSQL
                     AAAHTLTSAQVADRTTSMTAGGSSTISLTVAGTTTSLSVGSGSIDEIASAVNNAGLGV
                     RATVVNTGSGFRLQMSSASSGAASTFDLAG"
     gene            complement(646..1458)
                     /locus_tag="PLP95_04550"
     CDS             complement(646..1458)
                     /locus_tag="PLP95_04550"
                     /inference="COORDINATES: protein motif:HMM:NF012874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin"
                     /protein_id="HPB45104.1"
                     /translation="MRINNNVAAFNAWRNLSSTNTSMGKSLEKLSSGFRINRAADDAA
                     GLVISQNLRAQISGLKQATRNAQDGISVAQTAEGALTEVHSMLNRMRDLAVGAANTGT
                     MDANAIAANQAEFDELALQIDDIATKTKFGSQALLAGGFTGRVFQVGANNGDTITVSI
                     GDLQSGTLGVDTLSLTGAGAAAAITALDAAITTVSTERGKLGATQNRFESVINNLQVT
                     TENLVSSESRIRDVDMASEMTNFTKNQVLMQAGTSMLGQANSLSQSVLSLLQ"
     gene            complement(1738..2550)
                     /locus_tag="PLP95_04555"
     CDS             complement(1738..2550)
                     /locus_tag="PLP95_04555"
                     /inference="COORDINATES: protein motif:HMM:NF012874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin"
                     /protein_id="HPB45105.1"
                     /translation="MRINNNVAAFNAWRNLSSTNTSMGKSLEKLSSGFRINRAADDAA
                     GLVISQNLRAQISGLKQATRNAQDGISVAQTAEGALTEVHSMLNRMRDLAVGAANTGT
                     MDANAIAANQAEFDELALQIDDIATKTKFGSQALLAGGFTGRVFQVGANNGDTITVSI
                     GDLQSGTLGVDTLSLTGAGAAAAITALDAAITTVSTERGKLGATQNRFESVINNLQVT
                     TENLVSSESRIRDVDMASEMTNFTKNQVLMQAGTSMLGQANSLSQSVLSLLQ"
     gene            2800..3330
                     /locus_tag="PLP95_04560"
     CDS             2800..3330
                     /locus_tag="PLP95_04560"
                     /inference="COORDINATES: protein motif:HMM:NF016985.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FlgN"
                     /protein_id="HPB45106.1"
                     /translation="MTVADNTTTDIAASLDELSRVLWRQRELIELLDYRLEVQRLVLM
                     GGRPDHLQRALGDVETAMDEIRRLERNRDLVVRECAGRIGLAPDATLSMLKDSVAEPW
                     SSRLAEHQNALLALVAATERNAAANREIAAQGAAETRGVIDEITGTPSLSGYGPNGTR
                     NSGGGLSRPALVDREV"
     gene            3332..4753
                     /gene="flgK"
                     /locus_tag="PLP95_04565"
     CDS             3332..4753
                     /gene="flgK"
                     /locus_tag="PLP95_04565"
                     /inference="COORDINATES: protein motif:HMM:TIGR02492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
                     /protein_id="HPB45107.1"
                     /translation="MSTFGGLNTAVSGLNAHKRSIEVIGHNVANVNTEGFSRRQVVLE
                     PSVGLRTASRHNTGFSWSNLGVNIATVHRVRDAFLDARARSSAAGSSEANKLTEVLGT
                     LEAAMPEPGDNGLNTKLANFWNAFSDAANQPGATPQRTSVLAQGESLATGIRNAAESL
                     QVAHDNYSRELGDVIAEVNSLSDQVADLNSQIRQSTISGMNAADMMDQRDVLIDRLAA
                     LTGATTRPGEYNQTTVVLGGSTLVSGELREHLAVREIGPLNPPLNNLDPQMTQVQWAR
                     DGYAAASLGGTTGGIVAALNDVIPRYMNELDTVAQTLISTVNAQHQLGEGQDAVSGRN
                     FFDVSGPGPVSKRIMLSSDVAGQPQNIALAASGGGALDGSNGYALAALMTSATGANAS
                     YESMLGRLGIEGQAAASRAETQDQFAQQTETQRTSVAGVNVDEEMTNLMMSQRAYEAS
                     ARMLTAIDELLDVLINRTGIVGR"
     gene            4765..>5117
                     /locus_tag="PLP95_04570"
     CDS             4765..>5117
                     /locus_tag="PLP95_04570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB45108.1"
                     /translation="MMTRVATYGGSRSTLANIQRSFVRLDRAQEQVVTGKRVNRVSDN
                     PTDGVSSVINRTLLKRLEQYERNATEADAWLLANDSALADSATSLISARSRLVQVGGA
                     PDAVARKAIADELRAT"
BASE COUNT          844 a         1537 c         1802 g          934 t
ORIGIN      
        1 ccctgcgaga tcgaaggtgg atgccgcccc ggaggaggcg gaggacatct ggaggcggaa
       61 tccggatccg gtgttgacca cggtggcacg caccccgagc ccagcgttgt tgaccgccga
      121 ggcgatctcg tcgatcgatc cggaacccac cgaaaggctg gtcgtggttc ctgcgacggt
      181 gagggagatg gtgctcgaac ctccggcggt catcgacgtg gttcgatcgg ccacctgggc
      241 gctggtgagg gtgtgggcgg cggcgagttg ggagacggtg aaggtgagcg accctgccgc
      301 ggtcgtcgct ccgctggaga cctgggcggt ggcgagggcg atgttcgacg aggtggtggc
      361 gctcaacgac cagtcgttgg cgcggtcgag gttgttcgcg gcggtcgtca ccgcggtcat
      421 tttggtcagg atggagttgt aggcgtcgat cgccgactgc gccttggcct ggcgcgcctc
      481 catctggttg accgggatcc gctcgatcgc catcaggctg gtgatgattt tggcggtgtc
      541 gagaccgctg gccagaccgt caatcgccat ggcgggccac ctttcgtggg tgagcgccta
      601 cgggatgcga ggcaccatgt ctcgcatccc gtagtgctgg tggggttact gcaggaggct
      661 gagcaccgac tggctgaggc tgttggcctg gccgagcatg gaggttccgg cctgcatgag
      721 gacctggttc ttggtgaagt tggtcatttc ggaggccatg tcgacgtcgc ggatgcgtga
      781 ctccgaggac acgaggttct cggtggtgac ctgcaggttg ttgatgaccg attcgaagcg
      841 gttctgggtg gcgccgagct tgccgcgttc ggtcgacacg gtggtgatgg ccgcatcgag
      901 tgcggtgatc gccgccgcgg caccagcgcc ggtcagcgac agggtgtcca caccgagggt
      961 tccggattgc agatcgccga tcgacaccgt gatggtgtcg ccgttgttgg cgccgacctg
     1021 gaagacccga ccggtgaacc cgccggcgag cagcgcctgg gagccgaact tggtcttggt
     1081 ggcgatgtcg tcgatctgca gcgccagctc gtcgaattcg gcctggttgg cggcgatcgc
     1141 gttcgcgtcc atggtgccgg tgttggcggc gccgacggcg aggtcgcgca tgcggttcag
     1201 catggagtgg acctcggtga gcgcaccttc agcggtctgt gcgaccgaga tgccgtcttg
     1261 ggcgttgcgg gtggcctgct tgagtccgga gatctgggct cgcaggttct gggagatgac
     1321 caggcctgcg gcgtcgtccg ccgcgcggtt gatgcggaat cccgaggaga gcttttcgag
     1381 gctcttgccc atcgaggtgt tcgtgctgga caggttgcgc caggcgttga aggcggcgac
     1441 gttgttgttg atacgcatgg ttgtccaatc ctccgtgatt gggtgtggtg gagcggccac
     1501 gctcctcgtg gccgttgtcg gctcgggtcg agtcggcagg tgatgtatcg gccggcggcc
     1561 gccggttctg aagcactcat ccggacaatc tcggcaaatg ggccgctcgg cccgggcgag
     1621 tcgtcgagtt catcggctcc ggcggcggtc ttcgccaaag cgcgctcaag gggagcgagg
     1681 cgttgtgctt cgctcccctc gagtgttggg gcttcgggtt cctgggtgct ggtggggtta
     1741 ctgcaggagg ctgagcaccg actggctgag gctgttggcc tggccgagca tggaggttcc
     1801 ggcctgcatg aggacctggt tcttggtgaa gttggtcatt tcggaggcca tgtcgacgtc
     1861 gcggatgcgt gactccgagg acacgaggtt ctcggtggtg acctgcaggt tgttgatgac
     1921 cgattcgaag cggttctggg tggcgccgag cttgccgcgt tcggtcgaca cggtggtgat
     1981 ggccgcatcg agtgcggtga tcgccgccgc ggcaccagcg ccggtcagcg acagggtgtc
     2041 cacaccgagg gttccggatt gcagatcgcc gatcgacacc gtgatggtgt cgccgttgtt
     2101 ggcgccgacc tggaagaccc gaccggtgaa cccgccggcg agcagcgcct gggagccgaa
     2161 cttggtcttg gtggcgatgt cgtcgatctg cagcgccagc tcgtcgaatt cggcctggtt
     2221 ggcggcgatc gcgttcgcgt ccatggtgcc ggtgttggcg gcgccgacgg cgaggtcgcg
     2281 catgcggttc agcatggagt ggacctcggt gagcgcacct tcagcggtct gtgcgaccga
     2341 gatgccgtct tgggcgttgc gggtggcctg cttgagtccg gagatctggg ctcgcaggtt
     2401 ctgggagatg accaggcctg cggcgtcgtc cgccgcgcgg ttgatgcgga atcccgagga
     2461 gagcttttcg aggctcttgc ccatcgaggt gttcgtgctg gacaggttgc gccaggcgtt
     2521 gaaggcggcg acgttgttgt tgatacgcat ggttgtccaa tcctccgtga ttgggtgtgg
     2581 tggagcggcc acgctcctcg tggccgttgc cggctcgggt cgagtcggca ggtgatgtat
     2641 cggccgccgg ccgccggttc tgaagcactc atccggagaa atcgaacggt tgagtcgcgc
     2701 gacccggctg aggcgacggt cgtgggtccg atggccctcg cccgattgca gaggtcgccg
     2761 atcgctcagg ttctgaccga gctggccgat aacgacacca tgactgttgc cgacaacacg
     2821 acgaccgaca ttgccgcatc gctcgacgag ctgtcgcgcg tgttgtggag acagcgcgaa
     2881 ctcatcgagc tgctcgacta ccgcctcgag gtgcaacggc tcgtgctcat gggcggccga
     2941 cccgaccacc tccagcgtgc actcggcgac gtggagacgg cgatggacga gatccgccga
     3001 ttggagcgca accgcgacct cgtcgtacgc gaatgtgccg gtcgaatcgg tctcgcacca
     3061 gatgcgacac tgtcgatgct caaagattcc gttgccgaac cgtggtccag tcgactcgcc
     3121 gagcaccaga acgcattgct ggcgctcgtg gcggcgaccg aacgcaacgc tgcggccaat
     3181 cgcgagatcg cagcgcaggg ggctgcggag acccgagggg tgatcgatga gatcaccggc
     3241 accccgtcgt tgtccggtta cggccccaac ggaacccgaa acagcggtgg tggcctcagc
     3301 cgtccggcgt tggtcgatcg ggaggtttga gatgtcgaca ttcggcggac tcaacacggc
     3361 ggtcagcggg ctcaatgcgc acaagcgctc catcgaggtc atcggccaca acgtcgccaa
     3421 cgtcaacacc gaagggtttt cgcggcgaca ggtcgtgctc gagccgtcgg tcggtctgcg
     3481 gaccgcgtcg aggcacaaca cgggattctc ctggtcgaac ctcggggtga acatcgcgac
     3541 ggttcaccgg gtgcgcgacg ccttcctcga cgctcgcgcc cgttcgagcg cggccggatc
     3601 gtcggaggcc aacaagctga ccgaggtgct cggcacgctc gaagcagcga tgcccgaacc
     3661 aggtgacaac gggttgaaca ccaagctcgc caacttctgg aacgcgttct cagatgcggc
     3721 gaaccagccg ggggccaccc ctcaacgcac gagcgtgttg gcccaggggg agtcgttggc
     3781 gaccgggatc cgaaacgctg cggagagcct ccaggtcgct cacgacaact attcgaggga
     3841 gctgggcgat gtgatcgccg aggtgaactc gctcagcgat caggtggcag acctcaacag
     3901 ccagattcga cagtccacga tctcgggcat gaacgcggcg gacatgatgg accagcgcga
     3961 cgtattgatc gatcgacttg ccgcgttgac cggagcgacg acacgtccgg gcgagtacaa
     4021 ccagacgacc gtggtactcg gaggtagcac tctcgtgtcc ggagagttac gcgaacacct
     4081 cgccgtacgc gagatcggtc cgctcaaccc cccgctcaac aacctcgacc cgcagatgac
     4141 ccaggtgcag tgggcccgtg acggttatgc ggcagcgtcg ctcggcggca cgaccggcgg
     4201 catcgttgca gcattgaacg acgtcattcc gcgctacatg aatgaactcg acaccgtcgc
     4261 tcagacactg atctccacgg tcaacgcaca acaccaactc ggcgagggcc aagacgccgt
     4321 ctccggccgg aacttcttcg acgtcagcgg cccggggccg gtctccaagc ggatcatgtt
     4381 gagctccgac gtcgccgggc agccgcagaa catcgcgttg gcggcatcgg gaggcggtgc
     4441 cctcgacggg tcgaacgggt acgcgctcgc ggcgctcatg acctccgcta ccggcgccaa
     4501 cgcgtcgtat gaatcgatgc tcggtcgact cgggatcgag gggcaggccg ccgcttcccg
     4561 ggcggagacc caggaccagt tcgcacaaca gaccgaaacc caacggacgt cggtggccgg
     4621 ggtcaacgtc gatgaggaga tgaccaacct catgatgtcc cagcgcgcct atgaggcatc
     4681 cgcgcggatg ctgaccgcca tcgacgaact gctcgacgtg ttgatcaacc gcaccggcat
     4741 cgtcggacgt taggagccca tctcatgatg accagagtcg caacctacgg cggatcacgg
     4801 tcgacgttgg cgaacatcca gcggtcgttc gtccgcctcg accgcgccca ggaacaggtc
     4861 gtgaccggca aacgtgttaa ccgtgtgtcg gacaacccga ccgacggggt gtcgtcggtg
     4921 atcaatcgaa ccctgttgaa acgcctcgag caatacgagc gcaacgcaac cgaagccgac
     4981 gcctggctgc tcgccaacga ttcggccctc gccgacagtg cgaccagcct gatctcggct
     5041 cgcagccgac tcgtgcaggt cggtggtgcg ccggacgccg tcgctcgaaa ggccatcgcc
     5101 gacgaactcc gtgcgac
//
LOCUS       DAOOMA010000117         5097 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_19709_length_5097_cov_3.842919, whole genome shotgun sequence.
ACCESSION   DAOOMA010000117 DAOOMA010000000
VERSION     DAOOMA010000117.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 5097)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5097)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5097
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19709_length_5097_cov_3.842919"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            72..1046
                     /locus_tag="PLP95_04575"
     CDS             72..1046
                     /locus_tag="PLP95_04575"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016615 - malate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006108 - malate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HPB45109.1"
                     /translation="MRVAVTGAAGQIGYSLLFRIASGAMLGDDQPVILQLLEITPALE
                     ALRGVAMELDDCAFPLLAGIEQTDDANVAFGDIDVALLVGAMPRKDGMERSDLLSANG
                     GIFKPQGQALSANAKKDAKILVVGNPANTNALIAMNNAPDLDPGRFTAMTRLDHNRAM
                     TQLAQRTNTTVNDVKKMTIWGNHSATQFADLFHAEVNGQNAAELVNDQDWYANEYIPT
                     VAQRGAAIIKARGLSSAASAANAAIDHIRSWHLGTDEGDWVSMAIPSDGSYGVPEGLI
                     SSFPCVCKDGKYEIVQGLDINEFSQERIDKTVGELAEERDAVKELGLI"
     gene            complement(1176..1997)
                     /locus_tag="PLP95_04580"
     CDS             complement(1176..1997)
                     /locus_tag="PLP95_04580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB45110.1"
                     /translation="MSDNSDPTSLEPTGLEPTMNDGDEQRDLILAGAPRWADPTQVMQ
                     VDPHPTEPIAVAWGGAPVPTTAPAIASPTTSPQAGTLAGTLALPEQPAQPAMSPPYPP
                     FPLGGYPTPSSNAEELQARLAAAEAALVDQAARAHRRNVILIAGVVLFTVAASVGAVV
                     ITSWNNQRGDAATPVLPAPTTAAPRPTTAPTTSPRSTAPSTTAPRSTAPSTTEPSSSA
                     PAVPPAAPGNESTSGFDQWLDESGVGEYWDRIEKWSRDLEREADAWLNETFGGGR"
     gene            complement(2162..3001)
                     /locus_tag="PLP95_04585"
     CDS             complement(2162..3001)
                     /locus_tag="PLP95_04585"
                     /inference="COORDINATES: protein motif:HMM:NF013328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase family
                     protein"
                     /protein_id="HPB45111.1"
                     /translation="MPELPEIRVHAERLEAAYAGRVLTRTQAISFSVLKTFSPTPDAA
                     LGEALDSVGTRGKFFLLRFPSAVFVVHLMQGGRLTPDSKLSTKPRGGLFRWHFDQGPA
                     LLLTEPGTERKAGVWVIAPGDETVPPLDQQGPEADQVTPAMLDAILTEAKGARLHGVL
                     RDQHRIAGIGRRLANEVCWAAQISPFAPANKLGDDDRVRLMTALDRVIAAGFEDERTR
                     DHMANSADRASCVHRHTGDACPRCGDTIREVTYNAYQVNYCPSCQTKGKVLADNTTSK
                     FLK"
     gene            complement(3067..3498)
                     /locus_tag="PLP95_04590"
     CDS             complement(3067..3498)
                     /locus_tag="PLP95_04590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB45112.1"
                     /translation="MALQYLSDDWLDEAAALLARIVIEPPIDGPALALDTIVTGSDSG
                     DGSDDSAGDTVRFRYLFDGASVTLIREIPGEDADISVVRITQSRRVAIDIARGERSAQ
                     EAFLATDIQLGGDVTRLIQLAGLLDQVGDALAPLRATTEFR"
     gene            3541..4491
                     /locus_tag="PLP95_04595"
     CDS             3541..4491
                     /locus_tag="PLP95_04595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB45113.1"
                     /translation="MGTPSPSDERRHTPDDQPLWNESYYLDWFTDDGALGGYLRIGFY
                     PNLNRVWYWGCIVGEGRRLVMVTSHDAELPKDPQSLEVRADGLWADHSVDAPLEQFSV
                     NLESFALELDDPADMYHDQRGTLVPFGFELDFTTDRAAYLWPPIQTRMEIPCKVHGQV
                     MVGSDTIDIDAWGQRDHSWGGPRDWWMMGWMWSAGRLDDGTRFHAVGGFHEPGGGVSD
                     GTFGIAYLLDPDASSSTEFDEFDEVRVSAAFGHEGLADSATLEFGGLTLDVEPIAWAP
                     VLLVHDDGREARFPRALARFTARDGRRGHGWIEFNQPPTT"
     gene            complement(4552..5016)
                     /locus_tag="PLP95_04600"
     CDS             complement(4552..5016)
                     /locus_tag="PLP95_04600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB45114.1"
                     /translation="MGSPRRVLVGLGLVAALGCGLSGCSGDEDAKSDPTTTAADAVSG
                     QDGGGTSGDAPYDSSCVDASFVVSPSNAMSSMDEGVAAKMLENSQAFLETLTDEQREA
                     FTFVGQKANEVFVNGELDEQALAAQGSDPELQAAQAIVSGWFTEHCTVQAEG"
BASE COUNT          851 a         1720 c         1700 g          826 t
ORIGIN      
        1 aacctcggca tatgcgggcg gcatgggcgt cgcccaacaa acgaggagac tctgtgagca
       61 caaaagcccc catgcgtgtt gctgtcaccg gtgcggccgg ccagatcggg tacagcctgc
      121 tgtttcggat cgccagcggc gcgatgctcg gtgacgacca gcccgtgatc cttcaactgc
      181 tcgaaatcac cccggccctc gaggcgcttc gcggcgtggc gatggaactc gatgactgcg
      241 cattcccgct gctcgccggc atcgagcaga ccgacgatgc caacgtcgcg ttcggtgata
      301 tcgacgtcgc tctcctcgtc ggcgccatgc cgcgcaagga cgggatggag cgctccgatc
      361 tcctgtcggc caacggcggc atcttcaagc cgcagggcca ggcgctgtcg gccaacgcca
      421 agaaggacgc gaagatcctc gtcgtcggca acccggccaa caccaatgcg ctcatcgcca
      481 tgaacaacgc gcccgacctc gacccgggcc ggttcaccgc catgaccaga ctcgaccaca
      541 accgtgcgat gacccagctc gctcagcgca ccaacaccac ggtcaacgac gtcaagaaga
      601 tgacgatctg gggcaaccac tccgccaccc agttcgcgga tctgttccac gccgaggtca
      661 acggccagaa cgccgcagaa ctcgtcaacg accaggactg gtacgcgaac gagtacatcc
      721 ccaccgtcgc ccagcgaggc gccgccatca tcaaggcccg cgggctcagc tcggcggcct
      781 cggcggccaa cgctgcgatc gaccacatcc gcagctggca cctcggcacc gacgagggcg
      841 actgggtgtc gatggcgatc ccgtcggacg gttcctacgg cgtgcccgag ggactcatct
      901 cgtcgttccc gtgcgtgtgc aaggacggca agtacgaaat cgtgcagggg ctcgacatca
      961 acgagttctc ccaggagcgc atcgacaaga ccgtcggcga actcgccgag gaacgcgatg
     1021 cggtgaagga actcggcctg atctgaggcc gaccgttggc gcgctcgtgc gcctggacgc
     1081 ttaaccggta ggtagtgaat tggggcgcct ccttcgtggg gcgccccagt cgcgtcgttg
     1141 aacctcagtg gctcggaacc gcagggctca gtacctcagc ggcctccgcc gaaggtctcg
     1201 ttgagccagg catcggcctc gcgctcaagg tctcggctcc acttctcgat gcgatcccaa
     1261 tactcgccaa cgccgctctc atcgagccac tggtcgaacc cggaggtgga ctcgttgccc
     1321 ggcgcagcag gcggaacagc tggggccgaa ctcgacggct cggtcgttga cggggccgtc
     1381 gaacgcgggg cggtcgtcga cggggccgtc gaacgcggag acgtcgtcgg cgctgtggtg
     1441 gggcgggggg ctgcggtggt gggtgcgggc aggacggggg tggcggcgtc acctcgctgg
     1501 ttgttccagg aggtgatcac gacggcgccg accgacgcgg ccacggtgaa cagcacgact
     1561 ccggcgatga gaatgacgtt tcgccggtgc gcccgggctg cctggtcgac gagtgcggcc
     1621 tcggctgcgg ccaaccgggc ctgcaattcc tccgcgttcg aactcggggt cggatagccg
     1681 ccgagcggga acggcgggta cggcggcgac atcgccggct gagcaggctg ctccggcaga
     1741 gccagtgtcc cggccagtgt cccggcctgc ggcgaggttg ttggcgaggc gatcgctgga
     1801 gcggtcgtgg gaacgggcgc cccgccccag gccaccgcaa tcggttcggt cgggtgaggg
     1861 tcgacctgca tgacctgcgt cggatccgcc caccgaggcg ctccggcgag gatgaggtcg
     1921 cgttgctcgt cgccgtcgtt catcgtgggc tcgaggccgg tcggctcgag gctggtcgga
     1981 tcgctgttgt cggacatggc acacctaaac ggggtcaggg ccagcggccc gcggtcttcg
     2041 gcagccttcc ccttgtcgtc ggtcgttgcc agccgcgtcg ccaagagcgc tctcagacgt
     2101 cgaggatgtg ccgcccgcca ccgaccacag ccgccacgcc caccgccgag cgagcagcga
     2161 gctatttcag gaacttcgag gtggtgttat cggccagcac cttgcccttc gtctggcagc
     2221 tcgggcagta gttcacctgg taggcgttgt aggtgacctc gcggatcgtg tcgccgcagc
     2281 gaggacatgc gtcgccggta tggcggtgga cacaactcgc tcgatccgcc gagttcgcca
     2341 tgtggtcccg cgtgcgttcg tcctcgaagc cggcagcgat gacccggtcg agcgcggtca
     2401 tcagtcgaac acggtcgtcg tcgccgagct tgttcgccgg cgcaaacggc gagatctgtg
     2461 cggcccaaca cacctcgttg gcaaggcgcc tgccgatccc cgcgatccgg tgctggtcgc
     2521 gcaacacccc gtgaagacga gcccccttcg cctcggtcag gatcgcgtcg agcatcgccg
     2581 gggtgacctg atcggcctcg gggccctgct ggtcgagcgg cggcaccgtt tcgtcacccg
     2641 gagcgatcac ccagaccccg gccttgcgtt cggtcccggg ctcggtgagc aacagcgccg
     2701 gcccctggtc gaagtgccag cgaaacaagc cccctcgagg tttggtcgac agcttcgagt
     2761 cgggggtgag gcgcccgccc tgcatgaggt gcacgacgaa gaccgcactg gggaatcgca
     2821 acaggaagaa cttgccgcgt gtacccaccg agtccagcgc ttcgccgagc gcggcgtcgg
     2881 gagtcgggga gaaggtcttg agtaccgaga acgagatcgc ctgtgtccgt gtcagcaccc
     2941 gtcctgcgta cgcggcctcg aggcgttcgg cgtgaactcg gatctcgggc aactccggca
     3001 tcgcgccaac gtatcgcgcc ggggcacgcg ctcggccagc ggccagcggc cagcggcgtg
     3061 cggtcgctag cggaactcgg tggttgcgcg tagcggtgcc aacgcgtcgc cgacctgatc
     3121 gagcagcccg gcgagttgga tgagacgggt gacgtcgccg ccgagttgga tgtcggtcgc
     3181 caggaaggcc tcctgggcgc tgcgttcgcc tcgggcaatg tcgatcgcca cccgtcgcga
     3241 ctgggtgatt ctcacgaccg agatgtcggc atcctcaccc ggaatttccc gaatcaacgt
     3301 caccgacgca ccatcgaaca ggtaccggaa acgcaccgtg tcaccggccg aatcgtcgga
     3361 gccgtcgccg gagtcggagc cggtcacgat cgtgtcgagg gccagcgctg gcccgtcgat
     3421 cggcggttcg atcacgattc gggccaacag ggcggcggcc tcgtcgagcc aatcgtcgga
     3481 cagatattgc agcgccatcg gccacgcagc ctacttggcg accggttatg gtcggggcgc
     3541 atgggaaccc cgtcgccatc cgacgaacgc cgccacacac ccgacgacca gccgctgtgg
     3601 aacgagtcgt actacctcga ctggttcacc gacgacggcg cgctcggcgg ctacctccgc
     3661 atcggtttct accccaacct caatcgggtc tggtactggg gttgcatcgt cggcgagggc
     3721 cgtcggctcg tcatggtgac ctcccatgac gccgagctgc cgaaggatcc gcaatcgctg
     3781 gaggttcgcg ccgacgggct gtgggccgat cacagcgtcg acgccccgct cgagcagttc
     3841 tcggtcaacc tcgagagttt cgccctcgaa ctcgacgacc ccgccgacat gtaccacgac
     3901 cagcgcggaa cgttggtgcc cttcgggttc gaactcgact tcaccaccga cagagccgcg
     3961 tatctctggc cgccgatcca gacccggatg gagatcccct gcaaggtgca cggccaggtg
     4021 atggtcggct ccgacacgat cgacatcgat gcctggggcc agcgcgacca ctcgtggggt
     4081 gggcctcgcg actggtggat gatgggttgg atgtggagcg ccggacgcct cgacgacgga
     4141 acccgctttc acgcggtcgg cggattccac gaaccgggag gcggcgtgtc cgatggcacc
     4201 ttcggaatcg cctacctact cgaccccgac gcctcctcgt ccaccgagtt cgacgagttc
     4261 gacgaggtgc gggtgtcggc cgcgttcggt cacgaaggtc tcgcggactc ggcgacgctc
     4321 gaattcggcg gcttgaccct cgatgtcgaa ccgatcgcct gggctcccgt gttgttggtg
     4381 cacgacgatg gccgcgaggc gcgttttccg agggcactgg cgcgattcac cgcacgcgac
     4441 ggacgccgcg gacacggttg gatcgagttc aaccagccac cgaccacctg accgacgccc
     4501 ggtaaccggg cgccgtggag gcctcagccg ggagaccgca gccgggaggc ctcagccctc
     4561 ggcttggacg gtgcagtgct cggtgaacca gcccgacacg atcgcctggg cggcctgcaa
     4621 ctcggggtcg ctgccctgcg cagcgagcgc ctgttcgtcg agttcaccat tgacgaagac
     4681 ctcgttcgcc ttctggccga caaacgtgaa cgcttctcgc tgctcgtcgg tgagggtctc
     4741 gaggaacgcc tgggagttct ccagcatctt ggctgcgacc ccttcgtcca tcgatgacat
     4801 cgcgttggac ggtgacacca cgaacgaggc gtcgacacac gaagagtcgt acggggcgtc
     4861 accgctcgtg ccgccgccat cttgtcccga gaccgcgtcc gcggcggtag tggtcgggtc
     4921 ggatttggcg tcctcgtcac cgctgcatcc gctcaggccg caacccaacg ctgcgacgag
     4981 accaagaccg acaagaacgc gacgagggct acccatggca gccagcgtat cggtgaggta
     5041 ccccaaaaac gccgttctcc gataaccagt ggtcgtgatc acgaccactg gttatcg
//
LOCUS       DAOOMA010000118         5095 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_19717_length_5095_cov_4.136111, whole genome shotgun sequence.
ACCESSION   DAOOMA010000118 DAOOMA010000000
VERSION     DAOOMA010000118.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 5095)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5095)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5095
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19717_length_5095_cov_4.136111"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1271
                     /locus_tag="PLP95_04605"
     CDS             <1..1271
                     /locus_tag="PLP95_04605"
                     /inference="COORDINATES: protein motif:HMM:NF024594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4012 domain-containing protein"
                     /protein_id="HPB45115.1"
                     /translation="IALDHAPALLGAGGERRYLVLLGNPAELRDLGGHLGNWAELTIA
                     DGAINLTEVGRPSELSQPQLDAAVADDANVPESMVGLQPARYPQNWSGSLDFSLVAQM
                     SSRLFTAATGRSVDGVLYADPHVVSALLAITGPVEAPGLGRTIGSGDVVGFLTKDQFA
                     DYGTDSEGDDAVTEFIETVFERFQSSTLPGPRSIGDTFSPLVHAGRLRMISFHDDDQP
                     LLDLTGLSNTFDDTVTDYLAVVNRNANPSKIDAYLTRDNDYRVLWNPATGEVTATVTV
                     TLRNTAPATGLPDVVIGNGFGAPWGTNRTDLAVITPLGLKGVSVDNAEVGAMPLVEGR
                     GWRHTVRVDVPAGGERTVRFELKGRLQPARAYDLFIAAQPTLNPGSTNVTITSTDGSF
                     RPADGLDVLETEATTTVEDLGEHRLSAALR"
     gene            1453..2577
                     /locus_tag="PLP95_04610"
     CDS             1453..2577
                     /locus_tag="PLP95_04610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB45116.1"
                     /translation="MNVKKRTTAALSASAIVVALGAGLIPAGPVAAQGGTATTSCSEY
                     VAASLSVSNPSPNPGDTITVSGSTAPNSTVSIQLVIGSNVYLLGNVVTNASGSFSTSV
                     TIPADAPAGPARIQANDPACNGPLSVDITIGGGGTTTTTPTVGECGTDPITIHFDGTR
                     LIDWDGYNPKVEYTGIEPISLAAGTWNIIEAVSRDSYPGRVTQTQTSEIWEIEFLSGS
                     TVVARSAPTEDLADMIANAEWIGPLGSVTLTSAIDGVRAHHLPDQFPDAPWGQANSVD
                     PISFTICTEGGAAASTTTQAGQTTTTQAGQTTTTQAGDVDGNEGTAPSSAPSTTAGSG
                     VGGSNLAWTGSTTSVLLRIGAAAVMVGALILLKSRRQLAS"
     gene            complement(2856..4658)
                     /locus_tag="PLP95_04615"
     CDS             complement(2856..4658)
                     /locus_tag="PLP95_04615"
                     /inference="COORDINATES: protein motif:HMM:NF014740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate sugar
                     epimerase/dehydratase"
                     /protein_id="HPB45117.1"
                     /translation="MTFSTALVRFRFALQALFDATSWFVAIMAATLLRYDFDPSRIDF
                     VSVARFGLGIGLFQVVVGRFIGLYIHRWRYGTFEEVAAAIRSAAIVTVVSTLLNPVFD
                     RLVPVSVPILAGPIALCAMFGGRSAWRVIRDRTLRPDAETAKRVVVFGAGEGGAGLIR
                     SMLNNPNSPYVPVALLDDSPDVRNLWIRSVPVEGGRDDLAEVAKRHGATGLVIAIPSA
                     SGDLIRELVARADEAHLTTMVLPPVGELLGQLPSVGDVRPLTEADLLGRRELNTDIES
                     VAGYLTGRRVLVTGAGGSIGSELCRQIVRFAPESLVMLDRDESALQAVQVSIEGRGLL
                     DSRNLVVASIRDLDRMIEVFEEHQPDVVFHAAALKHLSLLEMHPEEGFKTNVLGTYNM
                     LEVAKRFGVSTFVNISTDKAADASSVLGRTKRLAERLTAHYAVGSDQSYLSVRFGNVL
                     GSRGSVIPLFRAQIARGGPVTVTHPDVTRFFMTIEEACQLVIQAGASSASGEVLVLDM
                     GEPVKITDLANRLIAESDRPIDIVYTGLRPGEKLHEVLFGPDEEAQARTSEHPLISRV
                     PVPALDPALLHDHIAEAVLPVRPATPVRPAHGTE"
     gene            4849..>5095
                     /locus_tag="PLP95_04620"
     CDS             4849..>5095
                     /locus_tag="PLP95_04620"
                     /inference="COORDINATES: protein motif:HMM:NF015666.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB45118.1"
                     /translation="MQRVVVIGQGYVGLPVAVRATEVDYDVVGFELDQRKVDGLNAGR
                     SHVEDITDERLGAAIATGRYRASADPAGSPPCAASALP"
BASE COUNT          948 a         1898 c         1543 g          706 t
ORIGIN      
        1 gcatcgccct cgaccacgcc cccgccctgc tcggcgccgg aggcgaacgc cgctacctcg
       61 tgttgttggg caacccggcg gagttgcgcg acctcggcgg acacctcggc aactgggccg
      121 aactcaccat cgccgacggc gccatcaacc tcaccgaagt cggtcgcccc tcagaactct
      181 cacaacccca gctcgacgca gcggtcgccg acgacgccaa cgtgcccgaa agcatggtcg
      241 ggttgcagcc cgcccgctac ccccagaact ggagcggatc gctcgacttc tccctcgtcg
      301 cacagatgtc gtcgcgcctg ttcaccgccg ccacggggcg cagcgtcgac ggcgtcctgt
      361 acgccgaccc ccacgtcgtg tccgcgctgc tcgccatcac cggccccgtc gaggcccccg
      421 ggctcggccg caccatcggc tccggcgacg tggtcggctt cctcaccaaa gaccagttcg
      481 ccgactacgg caccgactcc gagggcgacg acgccgtcac cgaattcatc gaaaccgtct
      541 tcgaacgctt ccagagctcc accctgcccg gcccccgaag catcggcgac accttctcac
      601 cgctcgtgca cgccggacgc ctccgcatga tctcatttca cgacgacgac cagccgctcc
      661 tggacctcac cgggctgagc aacaccttcg acgacaccgt caccgactac ctcgccgtcg
      721 tgaatcgcaa cgccaacccc tccaagatcg acgcctacct cacccgagac aacgactacc
      781 gggtgctgtg gaacccggcc accggcgagg tcaccgccac cgtcaccgtc acgctgcgca
      841 acaccgcacc ggccaccggg cttcccgacg tcgtcatcgg caacggattc ggagccccgt
      901 ggggaacgaa ccgcaccgac ctcgccgtca tcactcccct cgggctgaaa ggggtgagcg
      961 tcgacaacgc ggaggtcggc gccatgccgc tcgtcgaggg gcgcgggtgg cgccacaccg
     1021 tgcgcgtcga cgtccccgcc ggcggcgaac gcaccgtgcg attcgaactg aagggccgcc
     1081 tgcaacccgc acgcgcgtat gacctgttca tcgcggccca acccaccctg aatcccggct
     1141 cgacgaacgt caccatcacc agcaccgacg gatcgttccg gccggccgac gggctcgacg
     1201 tcctcgaaac cgaggcgaca accacggtcg aagacctcgg cgagcaccgc ctgtcggcag
     1261 cactcagatg aaccgcccca caccgcggtt cgagaacggg ccatttcacc tagcccccag
     1321 ccttgacccg aacctccgag tcgttcagac tctaagtcga atacgtcggg atagcatgag
     1381 agttcatcgc tcacccgacg gggctgttcg gcattcgaaa atgccggcgg cgggaaccac
     1441 aagggagtcc acgtgaacgt caaaaagcgc acaactgcag ccctttcggc ctctgcaatc
     1501 gttgtcgctc tcggtgccgg gctgatcccc gccggccccg tcgcggccca gggcggcacc
     1561 gccaccacca gctgttcgga atacgttgcg gccagcctgt cggtcagcaa cccgtctccc
     1621 aaccccggcg acacgatcac cgtcagcggc tccaccgcgc cgaacagcac cgtgtccatc
     1681 caactcgtca tcgggtcgaa cgtctacctg ctcggcaacg tcgtcacgaa cgcgagcggc
     1741 agcttctcca cctcggtgac catccctgcc gatgcccccg ccggcccggc gcgcatccaa
     1801 gcgaacgacc ccgcctgtaa cgggccgctc agcgtcgaca tcaccatcgg cggcggcggc
     1861 acgaccacca cgacgccgac cgtcggcgag tgcggcaccg acccgatcac catccatttc
     1921 gacggcaccc gcctgatcga ctgggacggc tacaacccca aggtcgaata cacgggcatc
     1981 gaaccgatca gcctggcggc aggcacctgg aacatcatcg aggccgtctc gcgagacagc
     2041 taccccggtc gagtcaccca gacccagacc tccgagatct gggaaatcga gttcctttcc
     2101 ggcagcacgg tcgtggcccg ctcggcaccc accgaagacc tcgcagacat gatcgccaac
     2161 gccgaatgga tcggaccgct cgggtcggtc acgctcacga gcgccatcga cggcgtgcga
     2221 gcccaccacc ttcccgacca gttccccgac gccccgtggg gccaggccaa cagcgtcgac
     2281 ccgatcagct tcaccatctg caccgagggc ggcgcagcgg cgagcaccac cacccaggcc
     2341 ggccagacga ccacgaccca agcgggtcag accaccacga cccaggccgg cgatgtcgac
     2401 ggcaacgagg gcacggcccc atcctcggct ccgagcacca cggctggtag cggagtcggc
     2461 ggcagcaacc tcgcctggac cggctcgacc accagcgtgc tgttgcgcat cggtgccgcg
     2521 gccgtgatgg tcggtgcgct catcttgttg aagtcccgac gccagctcgc ttcctgaggc
     2581 tgagcctcgc gcttggcgcc aagcgggtag cgctggcatc taacgctcaa acttcctgca
     2641 gaaagtgaca ccacgtgtca ctttctgcag gaagtttgcc gttcaggcac cccgagcggt
     2701 acgcgcctgg gcactcggca ccacccccag cccgaacgcc caccccagcc cgaacgcgcg
     2761 aagtttgtgc cagaagtgac accacgtgtc acttctggca caaacttcgc gcaggccccc
     2821 cgccggagcc gcccgaccga ctcggaggcg ggcgtttact cggtgccgtg agccgggcgg
     2881 accggagtgg ccggacgcac cggaaggacc gcctcggcga tgtgatcgtg caacaacgcc
     2941 gggtcaagcg ccggcaccgg cacccgcgaa atcagcgggt gctcggaggt gcgggcctga
     3001 gcctcctcgt cggggccgaa cagcacctcg tgcagcttct cacccggacg cagcccggtg
     3061 tacacgatgt cgatcgggcg atcggactcg gcgatcagac gattcgccag atcggtgatc
     3121 ttcaccggtt cgcccatatc gagcaccagc acctcacccg aggccgagct cgcgcccgcc
     3181 tggatgacca actggcaggc ctcctcaatc gtcatgaaga agcgggtgac gtcggggtgg
     3241 gtaaccgtca ccggaccgcc gcgtgcgatc tgcgcacgaa acagcgggat caccgaaccg
     3301 cggctgccca gcacgttgcc gaagcgcacg ctcagatacg actgatccga cccgaccgcg
     3361 taatgggccg tcagacgctc ggcgaggcgt ttggtgcgcc cgagcaccga ggacgcgtcg
     3421 gccgccttgt cggtggagat gttgacgaag gtcgacaccc cgaagcgctt cgccacttcc
     3481 aacatgttgt aggtgcccaa cacgttggtc ttgaacccct cctcggggtg catctcgagc
     3541 agggacaggt gcttgagcgc cgcggcgtgg aacacgacgt cgggctggtg ctcctcgaac
     3601 acctcgatca tccggtcgag gtcgcggatc gacgccacga cgaggttgcg cgaatcgagc
     3661 agcccgcggc cctcgatcga cacctgcacc gcctgcaacg ccgactcgtc gcggtccaac
     3721 atgacgagcg actcgggtgc gaaccgcacg atctggcggc acaactccga accgatcgat
     3781 ccgccggcgc cggtcaccag tacccgccgg ccggtcaggt agccggccac ggactcgatg
     3841 tcggtgttca actcacgtcg gcccagcaga tcggcctcgg tgagggggcg aacgtcgccc
     3901 accgacggaa gctgacccaa cagttcacca accgggggaa gcaccatcgt ggtcaggtgt
     3961 gcctcgtcgg cgcgtgccac caactcgcga atcaggtcgc ccgacgcgct cggaatggcg
     4021 atcaccagcc cggtcgcccc gtggcgtttc gccacctcgg cgaggtcgtc gcggccgccc
     4081 tcgacgggca ccgaccggat ccacaggttg cgcacgtcgg ggctgtcgtc caacaacgcc
     4141 accgggacat agggcgagtt gggattgttc agcatcgacc ggatcagccc ggccccgccc
     4201 tcgccggcgc cgaacacgac gacacgcttt gcggtctccg catcggggcg aagggtgcgg
     4261 tcgcggatga cgcgccacgc cgatcggcca ccgaacatcg cgcacagggc gatcgggccg
     4321 gccaggatcg gcaccgacac cggcaccaga cggtcgaaca ccgggttcag cagcgtcgaa
     4381 accacggtga cgatcgccgc cgaacggatg gcggcggcga cctcttcgaa ggtgccgtaa
     4441 cgccagcggt ggatgtagag cccgatgaag cggccgacca ccacctggaa caggccgatg
     4501 ccgagcccga aacgggccac cgacacgaag tcgatgcggc tcgggtcgaa gtcgtagcgc
     4561 aacaacgtcg ccgccatgat cgccacaaac cagctggtgg cgtcgaacaa cgcctgcagg
     4621 gcaaacctga aacgcaccag cgcggtggaa aaagtcatcg aagtggatcc ccagttgtgt
     4681 cccgaaccgt gcaataagct tcccccgcct gcggcgagga ttcaagaggc acgtcaaact
     4741 catctgcggc tgggtcgatc gaccctgtgg gcccgggtcg accgtcctgt tgaccctgcc
     4801 cgccacgctc cgcagactga tgcggcagcc gaaaaacgga ggacgttggt gcagcgagtt
     4861 gtcgtcattg gacagggtta cgtcgggctt ccggtggcgg tgcgcgccac cgaggtggac
     4921 tacgacgtcg tcgggtttga actcgaccag cgcaaggtcg acgggctcaa cgccgggcgc
     4981 tcccatgtcg aagacatcac cgacgaacgc ctcggcgccg cgatcgccac ggggcgctac
     5041 cgggcatcgg ccgaccccgc cggaagcccg ccctgcgcgg cctcggcgct gccca
//
LOCUS       DAOOMA010000119         5083 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_19780_length_5083_cov_3.510541, whole genome shotgun sequence.
ACCESSION   DAOOMA010000119 DAOOMA010000000
VERSION     DAOOMA010000119.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 5083)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5083)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5083
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19780_length_5083_cov_3.510541"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..733)
                     /locus_tag="PLP95_04625"
     CDS             complement(<1..733)
                     /locus_tag="PLP95_04625"
                     /inference="COORDINATES: protein motif:HMM:NF016923.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HPB45119.1"
                     /translation="MADISRAELSTLIEDAYSHVLLDSAQTTSAVLSAFTTVDMGTKT
                     SHLPVLATLPEASWVSETDTKPTAQVTWADKTLVAEELAVIIPVHENVVDDATVDVIS
                     QLSIQGGAAIGRALDAAVIFGTNKPVSWTSDDLLTSATHASQTFTVGADEDDLAGSIF
                     QAAGAVDEAGWDPDRLLVPRGIRYRLANLRSDDNAPIFLPSLSAAGGQDDVAGLTPAY
                     VSGRVWNRNEAEAIVVDSSRVIIGVR"
     gene            complement(779..1315)
                     /locus_tag="PLP95_04630"
     CDS             complement(779..1315)
                     /locus_tag="PLP95_04630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB45120.1"
                     /translation="MADEDQPAEDGGTDDSPADELNEGGKRAIDAERRKARDAEKRAR
                     TAETKLKDLEDAGKTETQKLRDDLADRDRQIAELPKQVRQQAVRFASEAARQGFLDPE
                     DAFAFLPADLDLDDTDAVKAALEDLAGRKPHLVRQAPAPKATARPTATGGEHLGSTQA
                     NESAKERAAAALRAFRST"
     gene            complement(1372..2109)
                     /locus_tag="PLP95_04635"
     CDS             complement(1372..2109)
                     /locus_tag="PLP95_04635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB45121.1"
                     /translation="MSVAGDLTIAQKTQQSALIDATLTVAARIWANLFDEKDPNGSWQ
                     RIEPLLVGLIQQRQPISSAVASNFIAALRSALEVPGRYVPTVAPAPTADDIVPWLRAA
                     GPAKAIELLNADRTGIAADVLESINGSITRQVLDGGRGTVSLNVDRDPRCLGYERHAS
                     GSCCSFCAMLTGRIYRSAESAGEGRDWHLKCRCGAVPVYSRDQAPPPNTQHYADLWAT
                     STRGLRGADARLAFRQAIEGREITAHP"
     gene            complement(2106..3440)
                     /locus_tag="PLP95_04640"
     CDS             complement(2106..3440)
                     /locus_tag="PLP95_04640"
                     /inference="COORDINATES: protein motif:HMM:NF016988.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HPB45122.1"
                     /translation="MLDAREAIELAGTLRKDAAPEWARLNRFSRYATGRQKGPWLPDS
                     AETEYKDIAKKSASNWLDLVVRSTAQGLYVVGYGDRSDESVTWEEGWQRNGMDARQHA
                     LFRAALVHGYAYLIVMPSDDGVWCRPDSATNVWAHFDDPFDDWAEYAVREVRKNHLEL
                     YDDEGRYSIIDGEVTFAPHRFGRCPVVRVLKAIDLIGPPQGEIEPVIPIQDRIVDATF
                     NLQMVSKYGAFPQRWIAGLDPSAPLVDESGLPLRDDDGNLIYPKIKAYVDSILTAVDP
                     NTKFGQFTAADLAQFVNALEAHIRHLAAITQTPPHYLLGSLVNLSAEALAAAEAGLQR
                     KIKEIRDVLGEGIEQAMRLMAHELGDEVAAEDPTARVRWRDVESRSFAQVADALVKLD
                     SIGVPKRKLFEMLPGWTDDDVEEAVAESKTELGMAAFAEIVTPAPQTEAPAA"
     gene            complement(3451..4944)
                     /locus_tag="PLP95_04645"
     CDS             complement(3451..4944)
                     /locus_tag="PLP95_04645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HPB45123.1"
                     /translation="MTASTRRLSEVARHLVIPEGIVTTGWPQVEAQCAEWGDEFDEWQ
                     RGLGKVMLGRRESGEYASTVGGITLSIPRQVAKTFLVGRTVFGLSAQFPGLKVLWTAH
                     RTRTATNTFRSLEGYAKRKAVAPHIKPGRRDGMGIRAANGEQEIPFRNGSNIMFGARE
                     TGFGRGFDEVDVIVFDEAQILTERALEDMVATTNQSRHPHGALLFFMGTPPRPIDPGE
                     AFTARRNEALAGEMVDGVYVECSADEKCNPDDRAQWAKANPSFPHRTPERSLLRLRKN
                     LPSNESWMREALGVWDDTSTKSAIPAAVWADLFDIDTAQPHPFALGVSMSADRKWTTI
                     GLAGHRSDGLIHLELLKVGRGSTWVIERLHQMERKPVGTGVLTTGPAGSLLPDIKNGR
                     GMTATELAAGCGRLLDSLGIRIAEDPERTVGEEAPLAEPTIRHSGQPVLNRAAGAVRL
                     KTLPKGAVTWLEPEGVDCSPIEAVTLALQVLTDGKSTPRSGRVQGIR"
BASE COUNT          719 a         1710 c         1790 g          864 t
ORIGIN      
        1 ggcggacacc gatgatgacc cgggacgagt cgacgacgat cgcctccgcc tcgttgcgat
       61 tccacacccg accgctcacg taggcggggg tgaggccggc gacgtcgtcc tgaccaccgg
      121 ccgccgagag cgacgggagg aagatcgggg cgttgtcgtc ggaccgcagg ttcgcgagcc
      181 ggtaccggat gccgcgaggc accaggagcc gatcggggtc ccagccggcc tcgtcgacag
      241 cgccggcggc ctggaagatc gaccctgcga ggtcgtcctc gtcggcgccg accgtgaagg
      301 tctggctggc gtgggtcgcc gaggtgagca ggtcgtcgga ggtccacgac accggcttgt
      361 tggtgccgaa gatcaccgcg gcgtcgaggg cccggccgat cgccgcgccg ccctggatgg
      421 acagctgcga gatcacgtcg accgtcgcat cgtcgaccac gttctcgtgg acagggatga
      481 tgacggcgag ctcctcggcg acgagggtct tgtcggccca cgtcacctgg gcggtgggct
      541 tcgtgtcggt ctccgagacc caggaggcct cggggagggt ggcgagcacc gggaggtgcg
      601 atgtcttggt ccccatgtcc accgtggtga acgcggagag gacggccgag gtcgtctgtg
      661 ccgaatcgag cagcacgtgt gagtacgcgt cctcgatcag cgtcgacagt tcggcgcggg
      721 agatgtcggc cattggggcc tcctttgtgg ttgacccgcc gtttggccga cggggttgct
      781 aggtggagcg gaacgcgcgc agggctgcgg cggcccgttc cttggcgctc tcgttcgcct
      841 gggtcgagcc gaggtgctca ccgccggtcg ccgtggggcg ggcggtggcc ttcggtgcgg
      901 gggcctgacg tacgagatgc ggcttgcggc cggcgaggtc ctcgagggcg gccttcacgg
      961 cgtcggtgtc gtcgaggtcg aggtcggcgg ggaggaacgc gaacgcgtcc tcgggatcga
     1021 ggaagccttg tcgtgcggct tcggatgcga aacggaccgc ctgctggcgg acctgcttgg
     1081 gcagctcggc gatctgccga tcacggtcgg cgaggtcgtc gcggagcttc tgggtctccg
     1141 tcttgccggc gtcctcgagg tccttgagct tggtctcggc ggtccgtgcg cgcttctcgg
     1201 cgtctcgggc cttgcggcgc tcggcgtcga tggcgcgctt gccgccctcg ttgagttcgt
     1261 cggcggggga gtcgtcggtc ccaccgtctt cggcgggctg gtcttcgtcg gccatcgcgg
     1321 cctccttgtt ttggtgcctc acgtcgcgtg aggacttggt gtgctcaccc ctcacgggtg
     1381 ggcggtgatc tcgcggccct cgatcgcctg tcggaaggcg agacgggcgt cggcgcctcg
     1441 gaggcccctc gtggaggtgg cccacaggtc ggcgtagtgc tgcgtgttcg gcggcggcgc
     1501 ctggtcccgg gagtacaccg gcacagcgcc gcaacggcac ttgaggtgcc agtcgcggcc
     1561 ttcgccggcg gactctgcgg atcggtagat gcggcccgtg agcatggcgc agaacgagca
     1621 gcaggagccg ctggcgtgac gttcgtagcc gaggcacctg ggatctcggt cgacgttgag
     1681 cgacacggtg ccacggccgc catcgagaac ctgacgggtg atcgacccgt tgatgctctc
     1741 gagcacatcg gcagcaatgc cggtgcggtc ggcgttgagc agctcgatgg cctttgctgg
     1801 gccggcggcg cggagccacg gcacgatgtc atcggctgtc ggggctggtg cgacggtcgg
     1861 gacgtagcgg ccgggcacct cgagcgccga gcggagagcc gcgatgaagt ttgacgcgac
     1921 ggcggaggag atcggctggc gttgctgaat gagcccgacg aggagcggtt cgattcgctg
     1981 ccacgaccca ttcgggtcct tctcgtcgaa caggttggcc cagatcctgg ccgccacggt
     2041 cagggtcgcg tcgatgaggg cggactgctg ggtcttctgg gcgatcgtga ggtcgccggc
     2101 gacgctcacg ccgccggcgc ctccgtctgc ggggctggcg tgacgatctc cgcgaaggca
     2161 gccatgccca gctcggtctt agactcagcg acggcttcct cgacgtcgtc gtcggtccag
     2221 ccggggagca tctcgaacag cttgcgcttc gggacgccga tggaatcgag cttcaccagc
     2281 gcgtcggcga cctgggcgaa cgaccgggat tcgacgtcac gccaccgcac acgggcggtg
     2341 gggtcttctg ccgcgacctc gtcgccgagc tcgtgggcca tgagacgcat cgcctgctcg
     2401 atgccctccc cgaggacgtc gcggatctcc ttgatcttgc gctgcagccc cgcctcggcg
     2461 gcggccaacg cctccgccga gaggttgacg agtgacccga gcaggtagtg gggcggggtc
     2521 tgggtgatcg ctgcgaggtg cctgatgtgc gcttcgaggg cgttgacgaa ctgggcgagg
     2581 tcggcggcgg tgaactggcc gaacttcgtg ttggggtcga cggcggtgag gatgctgtcg
     2641 acgtacgcct tgatcttcgg gtagatcagg ttcccgtcgt cgtcgcggag cgggaggccg
     2701 ctctcgtcga cgaggggtgc ggacgggtcg aggccggcga tccaccgctg cggaaaggcc
     2761 ccgtacttcg acaccatctg caggttgaac gtcgcgtcaa cgatccgatc ctgaatcggg
     2821 atgacgggct cgatctcgcc ctggggcggc ccgatgaggt cgatggcctt caggacccgg
     2881 accacggggc agcgcccgaa ccgatggggc gcgaacgtca cctcgccgtc gatgatcgag
     2941 tagcggcctt cgtcgtcgta gagctcgaga tggttcttgc ggacctcgcg cacggcgtac
     3001 tcggcccagt cgtcgaacgg atcgtcgaag tgagcccaca cgttcgtcgc cgaatcgggg
     3061 cggcaccaca cgccgtcatc ggagggcatg acgatcaggt aggcgtaacc gtgcaccagc
     3121 gcagcccgga acagggcgtg ctggcgggcg tccatcccgt tgcgctgcca gccctcttcc
     3181 cacgtcacag actcgtcgga gcggtcccca tagcccacca cgtagaggcc ctgggcggtt
     3241 gagcgaacga cgaggtcaag ccagttggaa gcggacttct tggcgatgtc cttgtactcg
     3301 gtctcggcgc tgtccggcag ccacgggcct ttctggcgcc cggtggcgta gcgggagaac
     3361 cggttgaggc gcgcccactc gggggctgcg tccttgcgga gggtgccggc cagctcgatg
     3421 gcctcgcgtg cgtcgagcat ggtcacctcc tcaccgaatc ccctgcaccc gccctgagcg
     3481 tggcgtcgac ttgccgtcgg tcaacacctg cagggcgagc gtcaccgcct cgatcggcga
     3541 gcagtccacg ccttcgggct cgagccacgt caccgccccc tttgggagcg tcttcaatcg
     3601 cacggcgccg gcggcacggt tcaacaccgg ctgcccggag tgccggatcg tcggttctgc
     3661 gagcggtgct tcctcgccca cggtgcgttc ggggtcctcg gcgatgcgga tgccgagcga
     3721 gtcgaggagc cgtccgcacc cggcggcgag ctcggtggcg gtcatgcctc gcccgttctt
     3781 gatgtccggc agcagcgacc cagccggccc ggtcgttagg acgccggtac cgaccggctt
     3841 gcgttccatc tgatgcagcc gctcgatcac ccacgtcgac ccacggccca ccttgaggag
     3901 ctcgaggtgg atcagcccgt cggaccggtg accggccagg ccgatcgtcg tccacttcct
     3961 gtccgccgac atcgacacgc caagggcgaa cggatgcggc tgggcggtgt cgatgtcgaa
     4021 caggtccgcc cacacggcgg ccgggatggc cgatttcgtg gaagtgtcgt cccacactcc
     4081 gagggcttcg cgcatccacg actcgttcga cggcaagttc ttacgcagcc gcaacaacga
     4141 ccgctcaggc gtccgatgcg ggaacgacgg gttcgccttc gcccactggg cccgatcgtc
     4201 ggggttgcac ttctcgtccg ccgaacactc gacgtacacg ccgtcgacca tctcgccggc
     4261 caacgcctcg ttccgacggg ctgtgaacgc ctcgccaggg tcgatcggcc gcggcggcgt
     4321 ccccatgaag aacaggagcg ccccgtgcgg gtgccgcgac tggttcgtcg tcgccaccat
     4381 gtcctcgagc gcccgctccg tcaggatctg cgcctcgtcg aacacgatca cgtcgacctc
     4441 gtcgaaccca cgcccgaacc cggtctccct ggccccgaac atgatgttcg acccgttgcg
     4501 gaacgggatc tcctgctccc cgttcgccgc ccgaatcccc atcccgtccc gcctgccagg
     4561 cttgatgtga ggggcgaccg ccttgcgttt cgcgtacccc tccaacgatc ggaacgtgtt
     4621 cgtcgccgtc ctcgtccggt gcgccgtcca caacaccttc aggcccggga actgggccga
     4681 cagaccgaac acggtccgac ccacgaggaa cgtcttcgcc acctgccggg ggatcgacaa
     4741 cgtgatcccg ccaaccgtcg acgcgtactc cccggactcc cgccggccca acatgacctt
     4801 gccgagaccg cgctgccact cgtcgaactc gtcgccccac tccgcacact gcgcctcgac
     4861 ctgcggccaa cccgtcgtca cgatcccctc ggggatcacg agatggcggg cgacctcaga
     4921 tagccgacgc gtcgaagcgg tcatccaccg aggccggcac caccgcacca ggatcagggc
     4981 gggccgtcag cgtctcgatc tccttcgaga tcatcgacaa ctgccggtgc agcgccgcct
     5041 tcgccggccc cttctcgtcg ggcagcgact tcacgatgtc gcg
//
LOCUS       DAOOMA010000120         5079 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_19798_length_5079_cov_2.975916, whole genome shotgun sequence.
ACCESSION   DAOOMA010000120 DAOOMA010000000
VERSION     DAOOMA010000120.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 5079)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5079)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5079
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19798_length_5079_cov_2.975916"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(6..1688)
                     /locus_tag="PLP95_04650"
     CDS             complement(6..1688)
                     /locus_tag="PLP95_04650"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPB45124.1"
                     /translation="MERTWTALRPYVPALGIVVLQVFVWPMSAGMAVSGAIMGGLGAM
                     IALGMAVIFRANNTVSFAQAELGALPATMAIVVMEVWGFTYWMSLVGGLFVALVVGTV
                     VEFLFVRRFFAAPPLLGTVATIGVAQLLLFASVSFPMIWNLAPKIRTYDAPFDVRWQL
                     GAVVFDANDLIAAVVAPTVMVALWAMLRFTNTGVALRASAANHQRASMLGIAVLRLQT
                     EVWAGAAAMSFLAVFLQAGITGLPPGGVMSWTILLRALAAMVLGNMVNLGAIAASSIA
                     LGILQTGMERSGDGAWVGPVLLAVVVIALLMQRRSEGRAGAGPSALSIGAEPHRLRGR
                     IAADRRVRALRWGIGVVVAMLVLAVPSMMGTAGTLKASAVVIFAIIGLSMVVLSGWVG
                     QVSLGQMTFAGIGAAVSAWAIVDRSLDPVVAMVLAAVCGAAVAVVIGVPALRLRGLHL
                     AVVTLALAVAASGAVFSNGIGEWIPTAGFSRPALAGRWSIDSPARLYYLAVAVAVAAG
                     LAVRGLRASRFGRVLIAQRDNERAAMSFGTGVVAAKLAAFGISGKVAFIGFS"
     gene            complement(1784..3499)
                     /locus_tag="PLP95_04655"
     CDS             complement(1784..3499)
                     /locus_tag="PLP95_04655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020378222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acyl-AMP ligase"
                     /protein_id="HPB45125.1"
                     /translation="MTEAPESTTPFRSDVPFDPKILTVAERLEVAATKGKNITVIDGD
                     THVTTSWAELHETAKEFAAALQARGVSEGTHVAIMGPTTRDLLVTIQAVWLAGGVLVM
                     LPLPMRMASVEGFIAQTRARIEFAKVSLVVLDPQLAAFLEPQEGDPAFVGYDDLRAAP
                     TGLSAADWKPPTPDPDALALLQFTSGSTSEPKGVMITHRQLCANMDAAAERARFTLDD
                     IVVSWLPLYHDMGLIGLCTIPMTTGTELVHAAPQDFLSKPARWLRWMHDFRGTITAGP
                     NFAYALATRSLKRAEGLDLSRIRFLLSGAEPIDAPTYRGFLDAAAVFGLDPNAAFPAF
                     GMAEVCIAATFPHPFTGLRTDVVEADALETDRRAVPAVLGGEVATRELARLGTPVRGM
                     RMRIVDVDTGEELDERRVGELQLAGTSLTPGYYENPEATADLFDGDWLHTGDLAYVAD
                     GELVICGRIKDVIIVGGRNIYPQDIEAAVGEVDGIRTGNAIAFGVDDSNSSQHIVVVA
                     EARTDDAVGLAKAVSRAVVAEVGVPPREVVLVKPGTIAKTSSGKLQRSQCRHEYRDGT
                     LERLG"
     gene            complement(3598..>5079)
                     /locus_tag="PLP95_04660"
     CDS             complement(3598..>5079)
                     /locus_tag="PLP95_04660"
                     /inference="COORDINATES: protein motif:HMM:NF012361.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HPB45126.1"
                     /translation="GTYGTGAIMAVPGGDQRDHDFAKAYGLDIIATVQPPADFEGEAY
                     AGDGEVINSEWLNGLSVDEAKAKAIEFLRSEGIGDAKVTFRLRDWLLSRQRFWGCPIP
                     MVYCDDCGLQPVPAQQLPVLAPDDVEFRPNGQSPLTFHEGFLNTSCPSCGGPARRETD
                     TMDTFVDSSWYYLRFADFQNDAAQMSPEVIAKWLPVDQYIGGAEHAVLHLMYARFFTK
                     ALSDLGLVPADLREPFKRLFTQGMIRLAGSKMSKSKGNLVAPEEIIDNQGADALRLAQ
                     LGLKPPQDDVDWEDVSIDGALRFLGRVWRLSVPGNDVIGEVRSGEPTAADQAIERARN
                     VVISEVTSDFDRWSYNTAVAKFMAFTNDLYRYVQSETGANGEVLQRSIDTLLLLMAPA
                     TPHVTAELWKRRVEAGISSGNVHIHRESWPTFEESKLVADTVTMVVQINGKVRERLEV
                     APDITEADAVAAALGTAKVAELVGGAEPRKVIARPPKLVNIVL"
BASE COUNT          889 a         1790 c         1623 g          777 t
ORIGIN      
        1 cgatgtcagg agaagccgat gaacgcgacc ttgcccgaga tcccgaacgc ggccagcttg
       61 gccgccacca cacccgtccc aaaggacatc gccgcgcgtt cgttgtcgcg ctgagcgatg
      121 agcacgcgac cgaatctgct cgcccgcagc ccccgaaccg ccaaccccgc cgccaccgcc
      181 acggccactg ccaggtagta gagccgcgcc ggcgagtcga tcgaccaccg cccggccaac
      241 gcgggtcgcg agaaccctgc ggtggggatc cactcgccga tcccgttcga aaacaccgcc
      301 cccgatgccg ccacggccag cgcaagggtc acgaccgcga gatgcagtcc gcgcagccgc
      361 agcgccggca ccccgatcac caccgccacc gccgctccgc acaccgccgc caacaccatc
      421 gcgaccaccg ggtcgaggct gcgatccacg atggcccagg cgctgactgc ggcgccgatt
      481 ccggcgaagg tcatctgccc gagcgacacc tgtccgaccc acccggacaa cacgaccatc
      541 gacagcccga tgatggcgaa gatcacgacc gccgaggcct tgagcgtgcc tgcggtcccc
      601 atcatcgacg gcacggcgag caccagcatc gccaccacga cgccgattcc ccaccgcagt
      661 gcccgaaccc ggcggtccgc agcgatacgg ccacggagtc gatgcggctc ggcaccgatg
      721 ctcaacgcgg agggtccggc accggcgcgg ccttcgctac ggcgttgcat caacagggcg
      781 atgaccacca ccgccaacag caccggtccc acccaggcgc cgtcgccgga tcgttccatg
      841 ccggtctgca agatgccgag ggcgatcgaa ctcgcggcaa tcgctccgag gttcaccatg
      901 ttgcccagca ccatggcggc cagcgctcga agcaggatcg tccagctcat gacaccgccc
      961 ggaggtaatc cggtgattcc ggcctgcagg aacacggcga gaaacgacat cgctgctgcg
     1021 ccggcccaca cctcggtctg caatcgcaac acggcgatac ccagcatcga tgcccgctga
     1081 tggttcgccg cgctcgcccg cagcgcgacg ccggtattgg tgaacctcag cattgcccac
     1141 aacgccacca tcaccgtcgg ggcgaccacc gccgcgatga ggtcgttggc gtcgaaaacc
     1201 accgccccga gttgccagcg cacatcaaac ggagcgtcat aggtgcgaat cttcggtgcc
     1261 aggttccaga tcatcgggaa gctgaccgac gcaaacaaca acagctgggc gaccccgatc
     1321 gtcgcgaccg ttcccagcag cgggggcgcg gcgaagaagc gtcgcacgaa gagaaactcc
     1381 accacggtcc ccaccacgag cgcgacgaac aggcctccga ccagcgacat ccagtaggtg
     1441 aatccccaga cctccatgac gacgatcgcc atcgtcgccg gcagcgcgcc gagttcggcc
     1501 tgggcgaaac tcacggtgtt gttcgcccgg aagatcaccg ccatacccaa cgcgatcatg
     1561 gcgccgagac cacccatgat cgcccccgaa acggccattc ccgccgacat cggccacacg
     1621 aacacctgca gcacaacgat gcccaacgca ggaacatagg gacgcaacgc cgtccaggtt
     1681 ctctccatct cgcacccctc cgatccaacc tccccccgaa ggtgtgcacg gatcgtgtcg
     1741 ggcactgtac cggttcggcc cggccacgcg gcgctttgtg cgactacccg aggcgttcga
     1801 gcgtgccgtc tcgatactcg tgtcggcact gcgaacgctg aagcttgccc gaggaggtct
     1861 tggcgatcgt gcctggcttg accagtacga cctcccgtgg cggcaccccg acctcggcga
     1921 ccaccgctcg cgaaacggcc ttggccaggc cgacagcatc gtcggtgcga gcctcggcga
     1981 ccacgacgat gtgctgtgac gagttcgagt cgtcgacccc gaaggcgatc gcgttgccgg
     2041 tccgtatgcc gtcgacctcg cccacggcgg cctcgatgtc ttgggggtag atgttgcggc
     2101 cgccgacgat gatgacgtcc ttgatccgac cgcagatgac cagttcgccg tcggcgacgt
     2161 aggcgagatc gcccgtgtgc agccagtcgc cgtcgaagag gtccgcggtc gcctcggggt
     2221 tttcgtagta ccccggggtc agcgaggtgc ccgcaagttg cagctcgccg actcggcgct
     2281 cgtcgagttc ctcaccggtg tcgacgtcga cgatgcgcat gcgcatgccg cgcaccgggg
     2341 tgccgagacg agcgagctcg cgggtcgcca cctcgccgcc gagcaccgcc ggcaccgccc
     2401 gacggtcggt ttcgagcgcg tccgcctcca cgacatcggt gcgcaggccc gtgaacgggt
     2461 gcggaaacgt tgcggcgatg cacacctccg ccatcccgaa ggcgggaaag gccgcgttcg
     2521 gatcgaggcc gaacaccgcg gccgcgtcga gaaacccgcg ataggtcggg gcgtcgatcg
     2581 gctcggctcc ggacagcaga aagcggatgc gcgacaggtc gaggccctcg gcgcgtttga
     2641 gcgaccgggt ggcgagcgcg tacgcgaagt tcggcccggc ggtgatcgtg ccgcgaaagt
     2701 cgtgcatcca ccgcagccac cgcgccggct tggacaagaa atcctggggg gcggcatgga
     2761 cgagttcggt gcccgtggtc atcgggatcg tgcacagccc gatcagaccc atgtcgtggt
     2821 agagcggcaa ccaggacacc acgatgtcgt cgagggtgaa ccgcgcccgt tcggccgcag
     2881 cgtccatgtt ggcgcacagc tgccggtggg tgatcatcac ccccttcggc tccgaggtcg
     2941 atccgctggt gaactgcaac agtgcgaggg catcggggtc gggtgtcggg ggcttccagt
     3001 cggcggcgct caacccggtc ggcgcggccc gcagatcgtc gtagccgacg aaggccgggt
     3061 cgccctcctg cggttcgagg aaggccgcca gttgggggtc gagcaccacc aacgacacct
     3121 tcgcgaactc gatgcgggca cgggtctgag cgatgaaccc ctcgacgctc gccatgcgca
     3181 tcggcaacgg caacatcacc aacactcccc ccgccagcca caccgcctgg atggtgacga
     3241 ggaggtcgcg ggtcgtcggc cccatgatgg cgacgtgggt tccctcgctc acgccgcggg
     3301 cctgcagtgc ggccgcaaac tccttggcgg tctcgtgcaa ctcggcccac gatgtcgtca
     3361 cgtgggtgtc gccgtcgatg acggtgatgt tcttcccctt ggtcgcagcg acctcaagtc
     3421 gctcagcgac ggtaaggatt ttcgggtcga acggaacatc gctccgaaac ggcgtcgtcg
     3481 attcgggcgc ctcggtcacg aacacgggta cctccgcggt gttgttgcat ctggccgttg
     3541 cggccgacct cacaactcac cccggcgggt cggctccggc gacccacggt agtgcgctca
     3601 cagcacgatg ttgaccaact tcggcggtcg ggcgatgacc ttgcggggct ccgccccacc
     3661 gacgagttcg gcgaccttgg cggtaccaag cgccgcagcg acggcatcag cctcggtgat
     3721 atcgggggcg acctcgaggc gctcgcgcac cttgccgttg atctgcacga ccatcgtcac
     3781 ggtatccgcc accagcttcg actcttcgaa ggtgggccag gactcccggt ggatgtgaac
     3841 gttgccgctg ctgatcccgg cctcgacgcg ccgcttccac agctccgcgg tgacgtgcgg
     3901 ggtggccgga gccatgagca acagcagcgt gtcgatcgag cgttgcagca cctcaccgtt
     3961 ggcacccgtt tcggactgca cgtagcggta caggtcgttg gtgaaggcca tgaacttcgc
     4021 caccgcggtg ttgtagctcc aacggtcgaa atccgaggtg acctcgctga tgaccacgtt
     4081 gcgggcgcgt tcgatcgcct ggtccgctgc ggtcggttct cccgagcgaa cctcgccgat
     4141 cacgtcgttt ccgggcacgg agagtcgcca cacgcgaccc aggaaacgca gcgcaccgtc
     4201 gatcgagacg tcctcccagt cgacgtcgtc ctgtggcggc ttgagtccga gttgtgcgag
     4261 gcgcagcgcg tcggccccct ggttgtcgat gatctcctcg ggggcgacga ggttgccctt
     4321 ggacttcgac atcttggatc cggcgaggcg gatcattccc tgggtgaaca ggcgtttgaa
     4381 cggctcgcgc aggtccgccg gcaccaggcc caggtccgac agcgccttgg tgaaaaagcg
     4441 cgcatacatg aggtgcagca ccgcgtgttc ggcgcccccg atgtactgat cgaccggcaa
     4501 ccacttggcg atcacctcgg ggctcatctg cgcggcgtcg ttctggaagt cggcgaaacg
     4561 taggtagtac cacgacgagt cgacgaaggt gtccatcgtg tcggtctcgc gtcgggccgg
     4621 ccccccacaa ctcggacagc tcgtgttcag gaagccttcg tggaaggtca gcggagactg
     4681 gccgttcggt cggaactcca cgtcgtcggg ggccaacacc ggcaactgct gcgcaggcac
     4741 gggctgcagg ccgcaatcgt cgcagtagac catcgggatg gggcaccccc agaaccgctg
     4801 gcggctcaac agccagtcgc gcaaccggaa ggtgaccttg gcgtcgccga ttccctcgga
     4861 gcgcaggaac tcgatcgcct tggcctttgc ttcgtcgacg ctgagcccgt tcagccactc
     4921 cgagttgatg acctccccgt cacccgcgta ggcctcaccc tcgaaatcgg cgggcggctg
     4981 aacggtcgcg atgatgtcga gtccgtaggc cttggcgaag tcgtggtcgc gctggtcgcc
     5041 gccgggaacc gccatgatgg cgcccgtgcc gtaggtgcc
//
LOCUS       DAOOMA010000121         5078 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_19804_length_5078_cov_3.428628, whole genome shotgun sequence.
ACCESSION   DAOOMA010000121 DAOOMA010000000
VERSION     DAOOMA010000121.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 5078)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5078)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5078
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19804_length_5078_cov_3.428628"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..763
                     /locus_tag="PLP95_04665"
     CDS             <1..763
                     /locus_tag="PLP95_04665"
                     /inference="COORDINATES: protein motif:HMM:NF014822.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alpha amylase C-terminal domain-containing
                     protein"
                     /protein_id="HPB45127.1"
                     /translation="HDTLDYLQRDSVHRRYHHNDLTFRAMYGTSERFVLPLSHDEVVH
                     GKASLASKMSGDDWQRAANLRMLFGMQWTTPGVPLLFMGGELAMNEEWNHDAELPWWL
                     LEFDKHRGVQRWVGELNSLQRAFAALRAPSTDGETFRWLDCADTDHSVVSWLRRSPAG
                     AGAGAGAGAGAGAGAGDVVVVANFTPTPHWGYRIALPGPGNWKLVANSDDRRWWGSGH
                     HVEPVLTAHEVGWAGWNWSTEMTLAPLAITIWSNR"
     gene            760..2190
                     /locus_tag="PLP95_04670"
     CDS             760..2190
                     /locus_tag="PLP95_04670"
                     /inference="COORDINATES: protein motif:HMM:NF015052.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3536 domain-containing protein"
                     /protein_id="HPB45128.1"
                     /translation="MTVYLCVHGHAYQPPRENPETGVVDRQPSAEPFHDWNERITDEC
                     YRRFGRAEVHGPDGGIATVMNLWGEVSFDLGPTLGVWMDRQTPDVAEMIAGGEATFGG
                     AMAHPWVHAILPLTPFWDRSTIVRWGIDDFEHRFGRQPAGMWLPETAVDVSSLEALVD
                     AGITWTLLAPHQVRRVGDAGTGGSAPGGSAPGGSAPGHRPLDVELPSGRSITVVPYDG
                     GLSHGIAFNGLLNDGVALAEAFLEAADRANDGDLIVAATDLETFGHHHAFGEMALAKA
                     VEVWVETDGVELISPERYLALAAQQGEPFERGELVAFTSWSCAHGVERWRSNCGCRFE
                     EVEGQDQSWRAPLRDALDTVAEVTRRILVQEAGAEFHDVWAARNAYGRVLAAGSSDAE
                     RDRRVQEFLAAPLVPGADAGRAIGWMEAERLRLEAWSSCAWFFDSLDRIETQQVIDEA
                     EVALEHYSELSGRPLNGLALADLVAS"
     gene            complement(2212..3387)
                     /locus_tag="PLP95_04675"
     CDS             complement(2212..3387)
                     /locus_tag="PLP95_04675"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: protein motif:HMM:NF012699.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid desaturase"
                     /protein_id="HPB45129.1"
                     /translation="MPSTTSLLLEDSRPPESSTRPERTLPDRLPTRGEVRAAIPDHCF
                     ERSTTRSFAWLGFSLAIVAALVAGAAALLPLSWAWAPVWVLYSVATGTAATGLWVIAH
                     ECGHGAFSRHDWVQNTVGFVLHSALLVPYFSWQRSHAVHHAKTNHLDEGETHVPKRIT
                     HANGRTAMRLRARLGRRGYTLVILVSTLLFGWPLYLLAGATAGPARRRANHFWPAAPF
                     RSELFPDRWVARVWMSSLGVLAAIALLITAGATFGWGLVAALYVGPYLVTNAWLVLYT
                     WLQHTDSDVPHFGGDDWSWMLGAFQTIDRPYPAVIDRLHHHIGTTHVLHHIDHRVPHY
                     HAREATEALKAAFPEWYRFNPTPIARATWQVAGDCVAVDLDADHSHNGWTFNAAERS"
     gene            complement(3618..>5078)
                     /gene="glgP"
                     /locus_tag="PLP95_04680"
     CDS             complement(3618..>5078)
                     /gene="glgP"
                     /locus_tag="PLP95_04680"
                     /inference="COORDINATES: protein motif:HMM:TIGR02094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucan family phosphorylase"
                     /protein_id="HPB45130.1"
                     /translation="GVSLLHGDVSRRMFSPLWPGIEIDDVPIGAVTNGVHAPTFMADD
                     IADIVARTVGDDWQTCDHERFSALSRVAPAELWAARSAGRSRLVDFVRERARRSVGRQ
                     LALNAAEAHGVASSNGDGAWIDTLLDPDTLTIGFARRFATYKRATMLLSEPDRLRHLL
                     TDPQRPLQLVFAGKAHPADEPGKDFLQAVARIAEDPVLRSKVVFLEDYDLDAGRMMTR
                     GVDVWLNTPLRPMEACGTSGMKAALNGVLNLSVRDGWWDEAYTPEVGWAIPTTDHAHL
                     DPVERDRIESTWLYEIIERQVVPLFYDRDPAGVPLGWTSMMASCIAALAPQFQAGRMV
                     RDYTLRHYVPAAERSTRLTADDASGARALAAWKTRVAAAWHEVHLAPAEPPVAAHVDE
                     EIEVHASLYLGSLSPDDVEVSVLVGDVAPDGDIASDEWLTLTDRGIDPDDGACRRYSA
                     HVVLDSPGSMGFAVRVVPRHPLLDRWSELALIRTAS"
BASE COUNT          794 a         1588 c         1879 g          817 t
ORIGIN      
        1 gcacgacacg ctcgactatc tgcagcgcga cagcgtgcat cggcgctacc accacaacga
       61 cctgacgttt cgtgcgatgt atgggacctc ggagcgtttc gtgttgccgc tgtcccacga
      121 cgaggtggtg cacggcaagg cctcgctggc atcgaagatg tccggcgacg actggcaacg
      181 cgccgccaac ctgcggatgt tgttcgggat gcagtggacg accccggggg tgccgctgtt
      241 gttcatgggc ggcgagttgg cgatgaacga ggagtggaac cacgacgccg agttgccgtg
      301 gtggctgctg gagttcgaca agcatcgtgg cgtgcagcgt tgggtgggcg aactcaactc
      361 gttgcagcgg gccttcgccg cgctgcgcgc ccccagcacc gatggcgaga ccttccggtg
      421 gctggactgc gccgataccg accacagcgt cgtgtcctgg ctccggcgaa gccccgcagg
      481 tgctggtgct ggtgctggtg ccggtgcggg tgccggtgcg ggtgccggtg acgtggtcgt
      541 cgtggcgaac ttcacgccga caccacactg gggctatcgc atcgcgctgc cggggccggg
      601 gaactggaag ttggtcgcca acagcgacga ccgacgttgg tggggatcgg gtcatcatgt
      661 cgaaccggtt ctgacggcgc acgaggtggg gtgggccggt tggaactggt cgaccgagat
      721 gaccctggca ccgctggcga taacgatctg gagcaaccga tgacggttta tctgtgcgtg
      781 cacggtcacg cctatcagcc cccgcgcgag aaccccgaga cgggggtcgt cgaccgccag
      841 ccgagtgccg agccctttca cgactggaac gaacggatca ccgacgagtg ctaccggcgt
      901 ttcgggcgcg ccgaggtgca tggccccgac ggtgggatcg ccacggtgat gaacctctgg
      961 ggggaggtga gtttcgacct cgggccgacg ctcggggtgt ggatggaccg ccaaaccccc
     1021 gacgtcgccg agatgatcgc cggtggggaa gcgaccttcg gcggggcgat ggctcatccc
     1081 tgggtgcacg cgatccttcc gctcacgccg ttctgggatc gttcgaccat cgtgcgatgg
     1141 ggcatcgacg atttcgagca ccggttcggc cgccaacccg ccgggatgtg gctgcccgag
     1201 acggcggtgg acgtgtcgag cctcgaggcg ctggtggacg cgggcatcac ctggaccttg
     1261 ttggcgccgc atcaggtgcg tcgggtcggc gacgccggga ccggtggttc ggctcccggc
     1321 ggttcggctc ccggtggttc ggctcccggg catcgaccgc tggacgtcga attgccgtcg
     1381 ggacgctcga tcaccgtcgt tccctacgac ggtgggctca gccatggcat cgcgttcaac
     1441 ggcctcctca acgacggcgt ggcgttggcc gaagccttcc tggaggcggc ggaccgagcg
     1501 aacgacggcg acctcatcgt tgccgccacc gatctggaga ccttcggtca tcaccatgca
     1561 ttcggcgaga tggccctggc caaggccgtc gaggtgtggg tcgaaaccga cggggtcgaa
     1621 ctgatttcac ccgagcgcta tctcgcgctc gctgcgcagc agggcgagcc attcgagcgc
     1681 ggtgaactgg tggcgttcac gagctggagt tgcgcccacg gggtcgagcg gtggcgttcg
     1741 aactgcggat gtcggttcga agaagtcgag gggcaggacc agtcgtggcg tgctccgttg
     1801 cgcgacgcgc tcgacacggt ggcggaggtg acccgtcgca tcctcgtgca ggaggcgggg
     1861 gccgagttcc acgatgtgtg ggcggcgcgc aatgcctacg ggcgggtgct ggcggcgggg
     1921 tcgtccgatg ccgagcggga ccggcgcgtt caggagttcc tcgcggcccc tctggtgccg
     1981 ggcgccgacg ccggccgggc gatcggttgg atggaggccg agcggctgcg ccttgaggcg
     2041 tggtcgagtt gtgcgtggtt cttcgactcg ctcgaccgca tcgagaccca acaggtgatc
     2101 gacgaagccg aggtcgcgct ggagcactac agcgaactca gcgggcgtcc cctcaacggc
     2161 ctggcgctcg ccgacctcgt ggcctcttga cccacgcggt gctcgcgtcg gctaggaccg
     2221 ctcggcggcg ttgaaggtcc acccgttgtg gctgtggtcc gcgtcgaggt cgacggcgac
     2281 gcagtcgccc gccacctgcc aggtcgcacg ggcgatcggg gtggggttga agcggtacca
     2341 ctcgggaaag gccgccttga gcgcctcggt cgcctcccga gcgtggtagt ggggaacccg
     2401 atgatcgatg tgatgcagca catgggtcgt gccgatgtga tggtgcaggc ggtcgatcac
     2461 cgcggggtaa gggcgatcga tcgtctggaa ggccccgagc atccacgacc agtcgtcgcc
     2521 gccgaagtgc ggaacgtcgc tgtcggtgtg ctgcagccag gtgtagagca cgagccaggc
     2581 gttcgtcacc agatacgggc cgacatagag ggccgcgacc aggccccatc cgaatgtcgc
     2641 ccctgcggtg atgaggagcg cgatcgcggc caggaccccg agactcgaca tccagaccct
     2701 ggcgacccag cggtcgggga acagctcgct gcgaaacgga gcggccggcc agaagtggtt
     2761 ggcccgtcgc cgtgccggcc ccgccgtggc cccggcgagt aggtacagcg gccatccgaa
     2821 caacagggtg gaaacgagga tcaccagggt gtagccgcgt cgacccagcc gggcccgcaa
     2881 ccgcatcgcg gtacgcccgt tggcgtgggt gatccgcttc gggacatggg tttcgccctc
     2941 gtcgaggtgg ttggtcttcg catggtggac ggcgtggctg cgctgccagg agaaatacgg
     3001 caccagcaac gccgaatgca gcacgaatcc gaccgtgttc tgcacccaat cgtggcgcga
     3061 gaacgcaccg tgcccgcatt cgtgggcgat gacccagagc cccgtggccg ccgtgccggt
     3121 ggccaccgag tacaacaccc acaccggagc ccacgcccag ctcaacggga gcagggcggc
     3181 ggcgccggca accagagcgg cgacgatggc gagtgagaac ccgagccacg cgaacgagcg
     3241 ggtggtcgag cgttcgaaac agtgatccgg gatggcggcg cggacctcgc cccgggtcgg
     3301 gagccggtcg ggcagggtcc gctcagggcg ggtcgaggat tccggcggac gggaatcctc
     3361 gaggaggagc gaggtcgtcg atggcatggg cgacaaggct agtgccgtgt ctgcgaacgt
     3421 ttgcacgttg cgacggccag atgactccgc tcgctgcgtt tcggactcct tgaattgggg
     3481 caagaccagt cctgcgtcgc cgcgccttgc gagcaccgcc cctggctcgc cgcaccatcg
     3541 caaacgtttc ttgccacggc actagatgcc tcagcaccgg gcgacagatt ccgacatgtt
     3601 cgtgacggtg tttcgtctca ggaagcggtg cggatgagtg ccaactccga ccaccgatcc
     3661 aggagcggat ggcgaggtac gacccgaacg gcgaacccca tagagccggg gctgtcgagc
     3721 acgacatgcg cgctgtatcg gcggcaggcg ccgtcgtcgg ggtcgatgcc gcggtcggtg
     3781 agcgtcagcc attcgtcgct ggcgatgtcg ccgtcggggg cgacatcgcc cacgagcacg
     3841 gagacttcga catcgtcggg gctgagcgac ccgagataca gcgaggcgtg cacctcgatc
     3901 tcctcgtcga cgtgcgccgc gaccggaggt tccgccgggg cgaggtggac ctcgtgccag
     3961 gcggcggcga cgcgcgtttt ccatgcggcg agtgctctgg cccccgacgc gtcgtcggcg
     4021 gtgagccgtg tggatcgttc ggccgcgggg acgtagtggc gcagcgtgta atcgcgaacc
     4081 atgcgtcccg cctggaactg cggggcgagc gcggcgatgc aggaggccat catggaggtc
     4141 caaccgagcg gcactccggc ggggtcgcgg tcgtagaaca acgggacgac ctgacgttcg
     4201 atgatctcgt agagccacgt cgactcgatg cggtcgcgtt cgacggggtc gaggtgggcg
     4261 tggtcggtgg tgggaatcgc ccagccgacc tctggggtgt aggcctcgtc ccaccagccg
     4321 tcgcgcaccg acaggttcaa cacgccgttg agcgccgcct tcataccgga ggtgccacac
     4381 gcctccatcg gacgcagcgg agtgttcagc cacacgtcga cgccccgggt catcatccgg
     4441 ccggcatcga ggtcgtagtc ttcgaggaac acgaccttgg agcgcagcac ggggtcttcg
     4501 gcgattcggg cgacggcctg aaggaagtcc ttgcccggct cgtcggcggg gtgggccttg
     4561 ccggcgaaga cgagttgaag cgggcgttgg gggtcggtga ggaggtggcg gagtcggtcg
     4621 ggctcgctca acagcatcgt cgcccgtttg taggtggcga agcggcgggc gaagccgatc
     4681 gtgagggtgt cggggtcgag gagggtgtcg atccaggccc cgtcgccgtt gctcgaggcc
     4741 accccgtgcg cctcggcagc gttgagtgcc aactggcgcc cgaccgagcg ccgggcgcgt
     4801 tctcgcacga agtcgaccag gcgtgatctg ccggcagatc gggccgccca cagttcggcg
     4861 ggggcgacgc gcgacagtgc cgagaaacgc tcgtgatcgc aggtctgcca gtcgtcgccg
     4921 acggtgcggg ccacgatgtc ggcgatgtcg tcggccatga acgtcggggc gtgcacgccg
     4981 ttggtgaccg ctccgatcgg cacgtcgtcg atttcgatcc ccggccacag cggcgagaac
     5041 atgcggcggc tgacgtcgcc gtgcagcagt gacacgcc
//
LOCUS       DAOOMA010000122         5053 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_19923_length_5053_cov_3.786715, whole genome shotgun sequence.
ACCESSION   DAOOMA010000122 DAOOMA010000000
VERSION     DAOOMA010000122.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 5053)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5053)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5053
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19923_length_5053_cov_3.786715"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..663
                     /locus_tag="PLP95_04685"
     CDS             <1..663
                     /locus_tag="PLP95_04685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB45131.1"
                     /translation="EIHEILCGGAARDPAEVPARPPAEVPAAPRPSLRWVIDTCSTAY
                     EGMEEHNALTFTPVEHPNPQVRQVGFDLEHPYVERCWSAVVGPSSMLLLRRVAALWVD
                     DVPARIDAAELSRSLGLGASVSGRSRLVNTLDRLVRFGLAQPARDGAGLDVHRQVAPL
                     SGRQLDRAPEWTRRAHDALLGTHLDQLATTPTQPLSMTARLDRLQHSPTPSDGPGQAV
                     GL"
     gene            803..4153
                     /gene="mobF"
                     /locus_tag="PLP95_04690"
     CDS             803..4153
                     /gene="mobF"
                     /locus_tag="PLP95_04690"
                     /inference="COORDINATES: protein motif:HMM:NF020332.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MobF family relaxase"
                     /protein_id="HPB45132.1"
                     /translation="MKFTVTPLGGGRADTARVVDSIVRYLQPPPKTTSASPEAAAGAG
                     GEGGAERYYADRGEQPGRWLGRTATVMGLTGEVHRSDFASVLAGRDPHSGERLISAQG
                     SAGRRAALGAGAHTKLSASGVRLYGVADAAAVLGVTHDEVERLLDAGAALHDVRREDP
                     GVAGHDEQGPARVPAEVPVGDRVAHPPPAEHPAEPPVWASGGGSSAGCVPGGSYLIPT
                     VETDGTRWVTDTELTRCSDARDAGVHPDEVRALGAPDDILTIAEAARLAGVTARYLRG
                     RARYHDEHRVEIERSITAGRTPRRAYLVAQRGVNGRWFVTRDDLAGFLERRRPPAVRV
                     AYDLTLTTEKSLGVLALLSDNTTREAVLGSIQAGNDWALNWIEDRALARVEGQPVPAG
                     GLAIASFQHLTSRALDPFPHHHNVIANTVQLPDGTHRGVWSRSLFVNAHGASALATAE
                     MRHRLSTQLGVRWRPGRTGGWEIAGITNAVIREFSQRRGEIDDALAELEAEIGRGAHP
                     GEIEHIVLRTRPAKNHTPADELITTWRKRAARHGLDPAACARITGHEPAEQHVDTATL
                     FASLAEPDGICAGGSVFTRTDALTALTNHPIPSGDDGVVQPLLCGADRLIELTDDFLA
                     SPHVVALTDTDDPLFTTTEMLAVQDRIATRFGNGLHRGAHQVAELHLDAAFATHPHLS
                     GEQRQLAIGWCQGGHRFQAAIGRAGAGKTTTVAACADAWTAAGYRVMGAAVKGEATRT
                     LAQATKIECETVAWYLAHTDPQSLPLDARTVLVVDEASTLSDRDLDALMDMAATTGAS
                     LRLIGDPAQHGAIAAGGMFRVLCERHEHATPELTTTHRLQHPHDRAAAQALREGRIDE
                     AFDLLAAAGHLHVVADDLAMYRHVLGRWWDAHRAGLEHPMVDRRNSTRRQLNRLAHLL
                     RRVNGELGSDPISASGDRTFAVGDRVTARAPNRDLHVNGDRHAYVRNGSLGTIIAVHP
                     HQRDAARDALTVDFDGIGRIDLPRSFFDHHRTTTGRGEVGIDHAYALTSYAVQGSTRD
                     VSTSRVDATSTRAETYVDITRGRGENHLYLTAVVDPLDGEALPKVPPAPADLAVAERL
                     HRSSGEKTAWELAHHADQPAAAERSIGI"
     gene            4225..4428
                     /locus_tag="PLP95_04695"
     CDS             4225..4428
                     /locus_tag="PLP95_04695"
                     /inference="COORDINATES: protein motif:HMM:NF024139.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HPB45133.1"
                     /translation="MTDTDTSVEVLTIDEAAAVLRISRNAAYAAARQWRDTRGEHGLP
                     CIEIGRTLRVPRADLDRMLGRPA"
     gene            complement(4470..>5053)
                     /locus_tag="PLP95_04700"
     CDS             complement(4470..>5053)
                     /locus_tag="PLP95_04700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB45134.1"
                     /translation="GGAETHLAEVADGPGVPLDTRATRVPRFVGRWSDSTLCGRTWNR
                     MAADADELLPEWRDPAFAPTCRGCLRILDSWFPTADTPSGVWLLAAVAAEEVTRFSST
                     YVTGVPVEHVEATRAAIRKVLRANGLGSSTRVVEGVVHVWSDDAYEAIDPAEIRDSVT
                     SAIERMTTGVDTAPDRDPDSTPGPIDWHTWVIE"
BASE COUNT          874 a         1990 c         1532 g          657 t
ORIGIN      
        1 gaaatccacg agattctgtg cggcggcgca gcgcgggatc ccgccgaggt tcccgctcga
       61 ccacccgccg aagttcccgc tgcaccccgc ccctcgctgc ggtgggtcat cgacacgtgc
      121 tcgacggcct acgagggcat ggaagaacac aacgccctca ccttcacccc cgtcgaacac
      181 ccgaacccgc aggtgcgaca ggttggcttc gacctcgagc acccctatgt cgaacgctgc
      241 tggtccgcag tcgtcgggcc cagctcgatg ctgctgctgc gccgcgtcgc cgccctgtgg
      301 gtcgacgacg tccccgcccg catcgacgca gctgagctgt cacgctccct cgggctcgga
      361 gccagcgtca gtggccgcag ccggctggtg aacacgctgg accgactcgt gcgcttcgga
      421 ctcgcccagc cggcccgcga cggcgccggc ttggacgtgc accgccaggt cgcacccctc
      481 tctggccgtc agctcgaccg ggcccccgag tggacccgac gagcccacga cgcgctgctc
      541 ggcacacacc tcgaccagct cgccacaaca ccgacccagc cgctgtcgat gaccgcccgc
      601 ctcgaccgtc tccagcactc acccacgccc tcggacggcc ccggacaagc cgtcggcctg
      661 tgaacccccc ttacccccaa gggcaccccg gaacacccgg ttgccggtcc cgtcacatcg
      721 acgcaagacg gcaggtccgg cgcgcacaca ttgacaggtt cccctccggg gaacctgttg
      781 aacctcccgc gcgggtcgtc tcgtgaagtt cacggtgacc ccactcggcg gcggcagagc
      841 cgacaccgcc cgcgtcgtgg actcgatcgt ccgctatctc caaccaccac cgaagaccac
      901 gtcggcgtcg ccggaagcag cggcgggcgc gggcggcgaa ggtggagcgg agcgctacta
      961 cgcggatcgt ggcgagcagc ccggccggtg gctcggccgc accgccaccg tgatgggact
     1021 caccggagag gtgcaccgca gcgacttcgc gtcggtgctc gcgggacgtg acccccacag
     1081 cggcgagcga ctcatctcgg cgcagggttc cgcagggcga cgagctgcgt tgggtgcagg
     1141 cgcccacacc aagctcagcg ccagcggtgt ccgcctttac ggcgtcgcgg acgccgctgc
     1201 ggtcctcggc gtcacccacg acgaggtcga acgtctgctc gacgccggtg cagcactcca
     1261 cgacgtacgc cgggaagatc ccggcgtagc cgggcacgac gagcagggac ccgcccgggt
     1321 tcccgctgag gttcccgtcg gcgatcgcgt ggcacaccca cctcccgctg aacatccggc
     1381 tgaacctccc gtttgggcgt ccggtggcgg aagttctgcc ggttgcgtcc cgggtgggtc
     1441 gtacctgatc ccgaccgtcg agacggacgg cacccggtgg gtgaccgaca ccgagctcac
     1501 gcggtgcagc gatgcccgcg acgcaggagt gcacccagac gaggtccgag cgctcggcgc
     1561 cccagacgac atcctgacga tcgccgaagc agctcgcctc gcaggagtca ccgcccgcta
     1621 cctgcgtggg cgtgcgcgct accacgacga acaccgcgtc gagatcgaac gatccatcac
     1681 agcgggacgc acaccgcgcc gcgcgtacct cgtcgcccaa cgcggtgtga acggccggtg
     1741 gttcgtgacg cgcgacgacc tggccgggtt cctcgaacgc cgacggccgc ccgccgtgcg
     1801 tgtcgcctac gacctcacct tgaccaccga gaagtccctc ggcgtactcg ccctcctcag
     1861 cgacaacacg acacgagagg ctgtgctcgg ttcgatccag gcgggcaacg actgggcgct
     1921 caactggatc gaagaccgcg ctctcgcccg cgtggagggc caacccgtgc cggcgggcgg
     1981 gttagcgatc gcatcgttcc agcacctcac ctcccgggcg ctggacccgt tccctcatca
     2041 ccacaacgtc atcgcgaaca ccgtgcaact ccccgacggc acacaccgcg gcgtctggtc
     2101 acgctccctg ttcgtgaacg cgcatggcgc ctcggcgctc gcgaccgcgg agatgcgtca
     2161 ccgactctcc acccagctcg gcgtgcggtg gcggcccggt cgaaccggcg gctgggagat
     2221 cgcgggcatc accaacgcgg tgatccgaga gttctcccag cgccgcggtg agatcgacga
     2281 cgccctcgca gaactcgaag cagagatcgg ccgaggggcg caccccggcg agatcgaaca
     2341 catcgtgctg cgcacccgac cagccaagaa ccacaccccc gccgacgaac tcatcaccac
     2401 ttggcgcaag cgcgcagcgc gccacgggct cgaccccgca gcgtgcgcca ggatcaccgg
     2461 acacgaaccc gccgaacagc acgtcgacac cgcgacgttg ttcgcgtcac tcgccgaacc
     2521 ggacgggatc tgcgccggag gctcggtgtt cacccgcacc gacgcgctca ccgcactcac
     2581 caaccacccc atcccaagcg gcgacgacgg tgtggtccag ccgttgctgt gcggcgccga
     2641 ccggctgatc gaactcaccg acgacttcct cgcgtcaccc cacgtcgttg cgctcaccga
     2701 caccgacgac ccattgttca ccaccactga aatgctcgcc gtgcaggacc gcatcgccac
     2761 acgcttcggc aacggcctgc accgcggcgc ccaccaggtc gccgaactgc atctcgacgc
     2821 cgcgttcgcc acgcaccccc acctcagcgg cgaacaacgc caactggcca ttgggtggtg
     2881 ccagggtggc caccgcttcc aagcagcgat cggccgcgcc ggtgccggca agaccaccac
     2941 cgtcgccgcg tgcgccgatg catggaccgc agccggctac cgggtgatgg gcgccgcggt
     3001 gaaaggcgaa gccacccgca ccctcgccca agcaaccaag atcgagtgcg agaccgtcgc
     3061 ctggtacctc gcgcacaccg acccccagtc cctccccctc gacgcccgca cggtgttggt
     3121 cgtcgatgag gcgtccacgt tgtcggaccg tgacctggat gcgctcatgg acatggccgc
     3181 caccaccggc gccagcctgc gcctcatcgg cgacccagcc caacacggcg ccatcgccgc
     3241 gggcggtatg ttccgcgtgc tctgcgaacg ccacgaacac gccaccccag agctgaccac
     3301 cacccaccgg ctccagcacc cccatgaccg cgccgccgcc caagcgttgc gagaaggtcg
     3361 tatcgacgaa gcgttcgacc tgcttgcagc ggccgggcac ctgcacgtcg tcgcggacga
     3421 cctcgccatg taccgccacg tcctcggccg ctggtgggac gcgcaccgcg ccgggctcga
     3481 gcacccgatg gtcgaccggc gcaacagcac cagacgccaa ctcaaccgcc tcgcccacct
     3541 cctgcgacga gtcaacggcg aactcggcag cgaccccatc tccgccagcg gcgaccggac
     3601 cttcgctgtc ggagatcggg tgaccgctcg ggcaccgaac cgcgacctcc acgtcaacgg
     3661 cgaccgccac gcgtacgtcc gcaacggctc gcttggaacc atcatcgccg ttcaccccca
     3721 ccaacgcgac gccgctcgcg acgcgctcac cgtcgacttc gacgggatcg gacgaatcga
     3781 ccttccccga tcgtttttcg accaccaccg caccaccacc ggccgcggcg aggttggtat
     3841 cgaccacgcg tacgcgctca cctcgtacgc cgttcaaggc tctacccgcg acgtgtccac
     3901 cagccgagtc gacgccacct ccacacgcgc cgagacctac gtcgacatca ctcgcggccg
     3961 cggggagaac cacctctacc tcaccgccgt agtcgacccc ctcgacggcg aagccctccc
     4021 caaagtcccc ccggcacccg ccgacctcgc ggtcgcagaa cgcctccacc gatccagcgg
     4081 cgagaagacc gcgtgggaac tcgcccacca cgccgaccag ccagcagcgg cggagcggtc
     4141 gatcgggatc tgaccaccgc acgcgacacc acgggccccg catcggctta cccagcacga
     4201 ccctcgatca gccaggagaa gaacatgacc gacaccgaca ccagcgtcga ggtgctcacc
     4261 atcgacgaag cagcagccgt gctccgcatc agccgaaacg ccgcctacgc agccgcacgc
     4321 cagtggcgag acacccgcgg tgaacacggc ctcccctgca tcgagatcgg ccgcactctc
     4381 cgtgtccccc gcgccgacct cgaccgcatg ctcggcagac cagcctgacg gcggtctcag
     4441 ccggtcaagc cgaccctcgg gctcaggagc tattcgatga cccaggtgtg ccagtcgatc
     4501 gggccgggcg tcgagtcggg atcgcgatcg ggcgcagtgt cgaccccggt cgtcatccgt
     4561 tcgatggcag aggtcacgct gtcacggatc tcggccgggt cgatcgcctc gtaggcgtcg
     4621 tcggaccaga catgcacgac gccctcgacg acccgtgtgc tggaaccaag gccgttggcg
     4681 cgaagcacct tgcggatcgc ggcgcgtgtc gcctcgacgt gctccaccgg gacaccggtg
     4741 acgtacgtcg agctgaacct ggtgacctcc tcggccgcca ccgcagccag cagccacaca
     4801 cccgagggag tgtcggctgt cgggaaccaa gagtcgagga tccgaaggca tccacgacag
     4861 gtcggagcga acgcgggatc gcgccactcc ggcaacaact cgtcggcatc ggcagccatc
     4921 cggttccagg tccggccaca cagcgtggag tcgctccagc gtccgacaaa gcggggcacc
     4981 ctcgtcgccc tcgtgtcgag cggaacaccc ggcccgtcgg cgacttcggc gagatgggtc
     5041 tcggcgccac cac
//
LOCUS       DAOOMA010000123         5032 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_20037_length_5032_cov_4.358047, whole genome shotgun sequence.
ACCESSION   DAOOMA010000123 DAOOMA010000000
VERSION     DAOOMA010000123.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 5032)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5032)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5032
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20037_length_5032_cov_4.358047"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1340)
                     /locus_tag="PLP95_04705"
     CDS             complement(<1..1340)
                     /locus_tag="PLP95_04705"
                     /inference="COORDINATES: protein motif:HMM:TIGR00976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HPB45135.1"
                     /translation="MTFTIVAVLAACVPPSPPDLSDTTGEYGVVVTRNLEIPLADGRV
                     LRADLYSPTDPLTGVPAPGPFPVIIGFTPYGKSGAATSPTGSDGVNLSLVRHGYLAAA
                     VDIPGTGASDGAFDLFDHHEAVAGAEVVDWAAQLPRSNGAVGMLGLSYMAIVQLFTAA
                     EVGPDSPLKAIFPMAATVDPYRDLYTSGGALNVLSPLGLLFGYGITRSVTPFTELWND
                     PIAALRHAGSNFGRLGEFEAVMADDMFNNGPRRYDGEWWDQRRPGRILQRIADNGVAV
                     YVVGGLYDVFQRGEPLIYSGLQNAWSGRDVEAPMAPDQPVSDRFQLLTGPWTHGTIGD
                     GLDMTAIQLSWFDRWLKGRNDSTHPLTSPAASPAAPLHVVEPGGASYDASTYPLDDAR
                     TERLWLREHGGLATGGPEAPEPDDVIYYSPLADPCTASTTQFAAGLFAECLAPVR"
     gene            complement(1543..3096)
                     /locus_tag="PLP95_04710"
     CDS             complement(1543..3096)
                     /locus_tag="PLP95_04710"
                     /inference="COORDINATES: protein motif:HMM:NF013300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004560 - alpha-L-fucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-fucosidase"
                     /protein_id="HPB45136.1"
                     /translation="MSKKRPDDDLPRRKRRVPAWWADAKLGIFVHWTPASVPGFAPTG
                     RDIGSLMVDPGPHPLSEIPYSEWYENSLRFENSSVARFHREHYGSRPYRAFADEFVAG
                     LERWDPTAWARTFAATGARYAVLVTKHHDGWCLWPSEVTNPHRVGWQSQRDLVGELGE
                     AVRGEGMRFGVYYSGAYDWSFDDSAIGTVGSGLRAIPGGEYVDYAEAHVRELIDRYRP
                     SVLWNDIGWPAPKARLDELFRYYFAAVPDGVVNDRWMPTGRLLKLAANPRLSGLVDRA
                     LRASAEKSGGLVPPEPPFYGYQTPEFVEFDEIRSTPFEIVRGFDRGFGYNQTSTEDDF
                     ISRRDLELLLVGAAATGGNLMANVGPRGIDASIPEEQMRRLRWLAEIEPLIAMALRDT
                     RPWVIPKFCDPHGTEVRFVARGESVFAWIWPSRTTTDGAAAETTTGSLVLPLRATART
                     RLLVADPDERTVDGSPSDGIAWCPLSIDTVHLGSRIVVPNDVMTPPATDDPRPRVLRV
                     DHAVAMPFS"
     gene            complement(3223..4770)
                     /locus_tag="PLP95_04715"
     CDS             complement(3223..4770)
                     /locus_tag="PLP95_04715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HPB45137.1"
                     /translation="MYPGDFARTTPDKAAIIMAATGAVTTYADLDEKANRLSHVLYDA
                     GLRPGDHVALCLENHEEFFPIMWGAHYLGLYYTALSSRLTTEEMAYIINDCGAKAFIT
                     SAYKADQAAELRDQMPEVSLRLMIDTELEGYDSYETAVAAASPEPLPDRTEGNDMLYS
                     SGTTGRPKGVKVPRSETPLGEVDDAVTFLCMMLFGANADTMYLSPAPLYHAAPLRYCR
                     SVQRLGGTVVVMDKFDPEAYLRLVEQYRITFSQIVPTMLIRMLKLPAQVRERYDVSSL
                     NCMVHAAAPMPVAAKEQGIEWFGPVIHEYYAGTEGNGFVYTNSEDWLAHRGTVGKAVV
                     GAIHIVGDDGEEVPTGESGTIYFESEALFEYHNDPTKTADSRDPKGRGWTTLGDVGRL
                     DEDGFLYLTDRKAYMIITGGVNVYPQEAENLLALHPKVADAAVFGVPNEDFGEEVKAV
                     VQPAEGVDGSPELEAELIAYCREHLADVKCPRTVDFRDELPRHPTGKLYKRVLRDEYW
                     AEAGRTI"
     gene            complement(4829..>5032)
                     /locus_tag="PLP95_04720"
     CDS             complement(4829..>5032)
                     /locus_tag="PLP95_04720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB45138.1"
                     /translation="TVAARLRGQQQTGIVRTDVDPDALADGSVGIILSVLMAVLQFGP
                     MAISTFGPGIVAMFDAALLPPEA"
BASE COUNT          827 a         1580 c         1775 g          850 t
ORIGIN      
        1 cgaaccgggg cgaggcactc ggcgaacagt ccggcggcga actgcgtcgt cgaagcggtg
       61 caggggtcgg cgagcgggga gtaatagatg acgtcgtcgg gctcgggggc ctcgggaccg
      121 cccgttgcga ggcctccgtg ctcgcgcagc cacagccgtt cggtgcgggc atcgtcgagc
      181 ggataggtcg atgcgtcgta actggcgccg ccgggctcca cgacgtgcag cggcgctgcg
      241 ggggacgcgg ccggcgacgt caacggatgg gtcgaatcgt tgcggccctt cagccatcgg
      301 tcgaaccagg agagttggat tgcggtcatg tcgaggccgt cgccgatcgt tccgtgggtc
      361 caggggccgg tcagcaattg gaaacgatcg gaaaccggct ggtcgggagc catgggagcc
      421 tcgacgtcgc ggccggacca cgcgttttgc aggccgctgt agatgagcgg ctcgccgcgc
      481 tggaagacgt cgtacagccc gccgacgacg tagaccgcaa caccgttgtc ggcgatgcgc
      541 tgcaggattc ggccaggtcg tcgttggtcc caccactcgc cgtcgtaccg gcgcgggccg
      601 ttgttgaaca tgtcgtcggc catgaccgct tcgaactcgc cgagacggcc gaagttgctg
      661 ccggcgtgac gaagcgcggc gatcggatcg ttccagagtt cggtgaacgg tgtgaccgag
      721 cgggtgattc cgtatccgaa caacagtccg aggggagaca gaacgttgag ggcaccgccg
      781 ctggtgtaaa gatcccgata cgggtcgacc gttgccgcca tcggaaagat cgccttgagc
      841 ggcgaatccg gcccgacctc ggcagcggtg aacagttgaa cgatcgccat gtaggaaagg
      901 ccgagcatcc cgactgcgcc gttcgaacgc gggagttgcg ccgcccagtc gaccacctca
      961 gcgccggcga ccgcttcgtg atggtcgaac aggtcgaatg ctccatccga tgccccggtg
     1021 ccgggaatgt cgaccgcggc cgccaggtag ccgtggcgca cgagcgacag gttgaccccg
     1081 tcggagccgg tcggtgaggt ggccgcgccc gacttgccgt atggggtaaa cccgatgatg
     1141 acgggaaagg gcccgggggc gggaaccccg gtgagtggat cggtcgggga gtagaggtcg
     1201 gcgcgaagca cgcgcccatc cgcgagggga atctcaaggt tgcgggtcac caccacgccg
     1261 tactcgccgg tggtgtccga caggtcgggc ggcgacggcg gaacgcacgc tgcgaggacc
     1321 gcgacgatcg tgaacgtcac cgtcacgcgg atcgagcaac gaagccggtt cttcgcgttc
     1381 cgttccttgg tagtcgtcgc gtccaacacg tcaccccgat cgtggtcgcc ggcgcagcca
     1441 tcccccgaga tgccccaccc tcggaggggc ggtgcgcctg tgcgcgccga agctacaggc
     1501 tcgtcgcttc ggctgcctcc gatgatcgag gcggcagagt gatcagctga atggcatcgc
     1561 taccgcgtgg tcgacgcgaa ggaccctcgg tcgggggtcg tcggtcgctg ggggtgtcat
     1621 cacgtcgttc ggcacgacga tgcgtgagcc gagatggacg gtgtcgatcg acaggggaca
     1681 ccaggcgatc ccatcgcttg gggaaccgtc gacggtacgc tcgtcggggt ccgcgacgag
     1741 cagcctcgtg cgcgccgtgg cgcggagcgg cagcaccaag gaacccgtgg tggtttccgc
     1801 tgcggcgccg tcggtggtcg tacggcttgg ccagatccac gcgaacaccg attcgccgcg
     1861 ggccacgaaa cgcacctcgg tcccgtgggg atcgcagaac ttggggataa cccacggtcg
     1921 ggtgtcgcgc agcgccatgg ctatgagtgg ttcgatctcc gcaagccagc gcagtcgtcg
     1981 catctgttcc tcggggatcg acgcgtcgat gccgcgcggt ccgacgttcg ccatgagatt
     2041 gccgcccgtt gccgccgctc ccacgaggag cagttcgagg tcgcggcgcg agatgaagtc
     2101 gtcctcggtc gacgtctggt tgtacccgaa cccccggtcg aacccgcgca cgatctcaaa
     2161 cggcgtcgag cgaatctcat cgaactcgac gaactcgggg gtctggtatc cgtagaacgg
     2221 cggctcgggt gggacgaggc cgccggactt ctcggcgctg gcccggagcg cacggtcgac
     2281 cagaccgctg aggcgcgggt tggccgcaag tttgagcaga cgaccggtcg gcatccaccg
     2341 gtcgttcacg acgccgtcgg gaaccgctgc gaagtagtag cgaaacaact cgtcgagccg
     2401 cgccttcggc gccggccagc cgatgtcgtt ccacagcacc gatggccggt agcggtcgat
     2461 gagttcgcgc acgtgggcct cggcgtagtc gacgtactcg ccacccggaa tggcgcgaag
     2521 tccggagccg accgttccga tggcggaatc gtcgaaactc cagtcgtacg cgccggagta
     2581 gtagacgcca aaacgcattc cctcgccgcg aaccgcctcg ccgagctcgc cgacgagatc
     2641 gcgttgcgac tgccatccga cacgatgggg gttggtgacc tcagagggcc acagacacca
     2701 gccgtcgtgg tgcttggtga ccaacacggc gtagcgggcg ccggtggcgg cgaaagttcg
     2761 cgcccaggcg gttgggtccc accgttcgag gccagccacg aactcgtcgg cgaaggcgcg
     2821 gtagggccgc gagccgtagt gctcacgatg gaaccgggcg accgagctgt tctcgaaacg
     2881 caacgagttc tcgtaccact ccgaatacgg gatctcgctg agcggatgcg gtcccggatc
     2941 gaccatgagg ctgccgatgt cgcgcccggt cggtgcgaac cctggcaccg acgccggtgt
     3001 ccagtgcacg aagatgccga gcttggcatc cgcccaccac gccggaacgc ggcgcttgcg
     3061 gcgggggagg tcgtcatcgg gacgcttctt ggacatcggt cgtctccaat cgccgttctc
     3121 cggcctaccg cctgtttccc gataaccagt ggtcgtgatc acgaccactg gttatcggga
     3181 aacaggctgg ggtggtcggg ctggggcggt caggtcgtgc ggttagatgg tgcggccggc
     3241 ctcggcccag tactcgtcgc gaaggacgcg tttgtagagc ttcccggtcg gatgccgcgg
     3301 gagctcgtcg cggaagtcga ccgtgcgggg gcacttcaca tccgcgaggt gttctctgca
     3361 gtaggcaatg agttcggctt cgagttccgg gctcccgtcg acgccttcgg ccggctgcac
     3421 cacggccttg acctcctcgc cgaagtcctc gttcgggacc ccaaagaccg cggcgtcggc
     3481 caccttcggg tgcagcgcga gcaggttctc cgcctcctgc ggatacacgt tcaccccgcc
     3541 ggtgatgatc atgtacgcct tgcggtcggt gagatacaaa aacccgtcct cgtcgagccg
     3601 gccgacatcg ccgagggtcg tccagcctcg acccttggga tcacgtgagt cagccgtctt
     3661 ggtcgggtcg ttgtggtact cgaacagcgc ctcggactcg aagtagatcg tgcccgattc
     3721 gccggttggg acctcttcgc cgtcgtcgcc gacgatgtgg atcgcgccca ccaccgcctt
     3781 tccgaccgtg ccgcgatggg ccaaccagtc ctcggagttc gtgtagacga atccgttgcc
     3841 ctccgtgccg gcgtagtact cgtggatgac ggggccgaac cactcgatac cctgttcttt
     3901 ggccgcgacc ggcatcggcg ccgccgcgtg caccatgcag ttcagactcg acacgtcgta
     3961 gcgctcgcgg acctgagcgg ggagcttgag catgcggatg agcatcgtcg gaacgatttg
     4021 gctgaaggtg atgcggtact gctcgacgag gcgcaggtag gcctcggggt cgaacttgtc
     4081 catcaccacg acggtgccgc cgagtcgctg aaccgagcgg cagtagcgga gcggcgcggc
     4141 gtgatagagc ggggccggag acagatacat cgtgtcggcg ttcgcgccga acagcatcat
     4201 gcacaggaag gtgacggcgt cgtcgacctc gccgagcggg gtctccgaac gcggcacctt
     4261 cacacccttc ggccgaccgg tggtgcccga gctgtacaac atgtcgttgc cctcggttcg
     4321 atcggggagc ggttcgggcg atgccgcggc gacggcggtt tcgtaggagt cgtagccctc
     4381 gagttcggtg tcgatcatga ggcgcagtga aacctccggc atctggtcgc gaagctcggc
     4441 tgcctggtcg gccttgtagg cggaggtgat gaacgccttc gcgccgcaat cgttgatgat
     4501 gtaggccatc tcctcggtgg tgagccgcga gctcagcgcc gtgtagtaga gcccgaggta
     4561 gtgcgcaccc cacatgatgg ggaagaactc ctcgtggttc tccaggcaca gcgcgacgtg
     4621 atcgcccggc cgaagcccgg cgtcgtagag gacgtggctg agccggttcg ccttttcgtc
     4681 gaggtcggcg taggtggtga cggcaccggt cgcggccatg atgatggcgg ccttgtctgg
     4741 tgtggttcga gcgaagtctc ccgggtacat ggcagaaccc taggtgcctc gatgccagtg
     4801 gcgtcgtcga tccgggtcgg ggtggcgttc aggcctcggg cggcagcagg gcggcgtcga
     4861 acatcgccac gatgccgggg ccgaatgtcg agatggccat cgggccgaac tgaagcaccg
     4921 ccatgaggac cgacaagatg atgcccaccg acccatccgc gagcgcatcg gggtcgacgt
     4981 cggtgcgaac gattccggtc tgttgttgtc cccggagcct ggccgccacg gt
//
LOCUS       DAOOMA010000124         5028 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_20065_length_5028_cov_3.755279, whole genome shotgun sequence.
ACCESSION   DAOOMA010000124 DAOOMA010000000
VERSION     DAOOMA010000124.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 5028)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5028)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5028
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20065_length_5028_cov_3.755279"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..873
                     /locus_tag="PLP95_04725"
     CDS             <1..873
                     /locus_tag="PLP95_04725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HPB45139.1"
                     /translation="LAEITADILPPGVLNVVCGTGASVGSALVNHPRVDMISLTGGVA
                     TGKRIAAEAAASLKRLHLELGGKAPVVIFEDADLDLVVETLTEMSYYNSGQDCTQPAR
                     YLAAASIYDELVERMTAAVAGLATGDPFDPDTAVGPIVSAAHQQRVTDMIARAKADGA
                     TVTTGGAAIDGPGFFVEPAVIAAAAQDSEIIQREIFGPAVTIQRFGSEDEALAMANDC
                     DYGLASSVWTGDVGRAMRFSRDLHFGAVWVNDHIPIVSEMPHGGYKQSGYGKDMSIYA
                     VEHYTELKHVMIRH"
     gene            951..1430
                     /locus_tag="PLP95_04730"
     CDS             951..1430
                     /locus_tag="PLP95_04730"
                     /inference="COORDINATES: protein motif:HMM:NF021221.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4870 domain-containing protein"
                     /protein_id="HPB45140.1"
                     /translation="MSFDPNASGTPTPPGWYPDGTGSQRYWDGTQWTEHLAPGAPQHG
                     GMQPYGGGGDDTTFATLVHILALFTSFIGPLIIYLVKGEESPFVKHHAAEALNFGITV
                     FIAYAVSAVLMIVLIGFVLLPLVAIWGIVMPIMAALAANKGEWYRYPLTLRLVPGGR"
     gene            complement(1468..2454)
                     /locus_tag="PLP95_04735"
     CDS             complement(1468..2454)
                     /locus_tag="PLP95_04735"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HPB45141.1"
                     /translation="MTFVTARPITVKDPTYGSITLSVHEQGDGPAVVFSHGFPELAYS
                     WRHQLPAVAAAGFHAIAPDQRGYGASSAPEEIRAYGLGQLTGDLVGLLDALDIERAVF
                     VGHDWGGFVVWAMGVLHPQRVAGIVGVCTPYTPFPGTEFLKMVFGDDVEKQYMLWFQK
                     PGVAERVLDPRGRLVFEKLMRGGVDPTNFLKSAATGGGEAKRPAMNPFINIETIDPGG
                     KPIATAEEIGRYVAEFERTGFRGGINWYRNIDANGEAFPEIGKTVIDVPTLMILAEWD
                     PALPPALAKNMPSVCSDLEMATIAKAGHWVQQESPDEVNAVLIDWLTRRFGG"
     gene            2558..3706
                     /locus_tag="PLP95_04740"
     CDS             2558..3706
                     /locus_tag="PLP95_04740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB45142.1"
                     /translation="MAASRFGQSTSIAGERWALSLGVTDEVLNLAAAFYIGDGIAAGE
                     LNRRFTLPLMGEVDLSLALTITGVEFTSSPDHDGRLHASIRAAGSVDFGAGAAMPALP
                     GRALVRADVLVSPRVELRPDGRFVAALDLEGAELLRTVMEGIEGSEVDPGTAAVMGDM
                     LFASIGGDIFSGLAAQMGRIGIELEPHQAQPLVDLGVRAAPGDVVIDEGHMTVGLIAV
                     DSVEGHAIAPLRPGQDVVVGLASGSLTQLALRLAEDSLGVPLPFEVDLQTHSSEVAAR
                     IRNRRLTSLGFLPDLRTGVRASVAARLLDDRIELSPREAWVELPLVPSFVNRFNQALG
                     SLASLTPFQVSLPAKVSVPIDDSTTVAVRATELAMRPDGVVCVLEADL"
     gene            3703..4530
                     /locus_tag="PLP95_04745"
     CDS             3703..4530
                     /locus_tag="PLP95_04745"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: protein motif:HMM:NF024744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004721 - phosphoprotein phosphatase
                     activity [Evidence IEA];
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein phosphatase"
                     /protein_id="HPB45143.1"
                     /translation="MSNPEPQTPSDLDPEAPSAGETNQPIPAIPGAFNLREFGGYRVA
                     GGAIRPGLLYRSDLLNRVDPRRAHDWLGVAGVATLVDLRTEAERGADGFVESNNDLES
                     IHRSLLDEVWSWRDEDESDSEWFLRDRTISMYEQHSDRIVVVLEDLVARDGAALFHCT
                     AGKDRTGVVASALLGVLGASDEVIIADYSKSTEAMVGIVGWYRDRMIEQARAQGLDAA
                     PPGRLDEEEIIRRAAAPATMRGVIDALKARHGSFEGWAVDIGVDRRLIDALRAKFVH"
     gene            4579..>5028
                     /locus_tag="PLP95_04750"
     CDS             4579..>5028
                     /locus_tag="PLP95_04750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB45144.1"
                     /translation="MTETLPQIDLLNGYLYAADPTPTYRWLRHHAPVYRDDINRLWGI
                     SRYHDIVEVEKRPRRYSNRNSYRPIPVGTYDDRSMIGLDDPDHQAQRRLVARRFTPRA
                     VVDHDAEITEVIDGLIDEALAAGTVDAVEALAAPLPAMMIAKYLGFGI"
BASE COUNT          823 a         1650 c         1720 g          835 t
ORIGIN      
        1 ctcgccgaga tcaccgccga catcctgccg ccgggcgtgt tgaacgtcgt gtgcggcacc
       61 ggggcgagcg tcggatcggc gctggtcaat cacccccgcg tcgacatgat cagcctcacc
      121 ggtggggtcg ccaccggcaa acgcatcgcg gcggaggccg ccgcgagcct caaacgcctg
      181 catctcgaac tcgggggaaa ggcaccggtg gtgatcttcg aggacgcgga cctcgacctc
      241 gtcgtcgaga ccctcaccga gatgagctac tacaactccg gccaggactg cacccaaccg
      301 gcccgctacc tcgcggcggc gtcgatctac gacgagttgg tcgagcggat gacggcggca
      361 gtggccggcc tcgcgacggg agatccgttc gatccagaca ctgcggtggg gccgatcgtg
      421 agcgcggccc accagcaacg ggttaccgac atgatcgcca gggccaaggc ggacggcgcg
      481 acggtcacca cgggtggtgc cgcgatcgac ggtcccgggt tcttcgtcga accggcggtg
      541 atcgccgcag cggcgcagga ctccgagatc atccagcggg agatcttcgg cccggcggtc
      601 accatccagc ggttcggatc cgaggacgag gcgttggcga tggccaacga ctgcgactac
      661 gggctggcgt catcggtgtg gaccggtgac gtcggccggg cgatgcggtt cagccgagat
      721 ctgcacttcg gtgcggtgtg ggtgaacgac cacattccga tcgtgtcgga gatgccccac
      781 ggcggctaca agcagtcggg gtacggcaag gacatgtcga tctatgcggt cgagcactac
      841 accgagttga agcacgtcat gatccgccac tgaaccggcg tcgacccgtc ggcgagaacg
      901 ccgggcaaac gaggctccgc tcggcggaac cacggcgata cgctgacgcg atgagcttcg
      961 acccgaacgc atccggaacc ccgacgcccc ctgggtggta ccccgacggc accggaagtc
     1021 agcgctattg ggacgggacg cagtggaccg aacacctcgc gccgggggca ccgcagcacg
     1081 gcggcatgca gccctacggc ggtggaggag acgacacgac gttcgcgacg ctcgttcaca
     1141 tcctggcgct gttcacgtcg ttcatcggcc cgctgatcat ctacctggtc aagggtgagg
     1201 aatcaccgtt cgtgaagcac cacgccgcgg aggcgttgaa cttcggtatc accgtgttca
     1261 tcgcgtacgc cgtgtcggcc gtgctgatga tcgtgctgat cggatttgtg ctgttgccgc
     1321 tggtggccat ctggggcatc gtgatgccga tcatggcagc gctcgccgcg aacaagggcg
     1381 agtggtaccg ctacccgctg acgttgaggc tcgtcccggg cggccgctag gccggtaggc
     1441 cagccccgac gggccgaccc cgtgcgctca gccgccgaag cgacgggtga gccagtcgat
     1501 caacacggcg ttcacctcgt ccggcgattc ctgttgcacc cagtggccgg ccttggcgat
     1561 ggttgccatc tcgaggtcgg agcacacgga cggcatgttc ttggccagcg ccggaggcag
     1621 cgccggatcc cactcggcca ggatcatgag cgtcggcacg tcgatgacgg tcttgccgat
     1681 ctcggggaag gcctcgccgt tggcgtcgat gttgcggtac cagttgatac cgccgcgaaa
     1741 gccggtccgt tcgaactcgg cgacgtagcg gccgatctcc tcggccgtcg cgatcggctt
     1801 gcctccgggg tcgatggtct cgatgttgat gaacgggttc atcgccggtc gtttcgcctc
     1861 gccgccgccg gtcgcggccg acttcaggaa attcgtcggg tccacgccgc cgcgcatgag
     1921 cttttcgaac acgagccggc cccgcgggtc gagcacccgc tcggcgacgc cgggcttttg
     1981 aaaccacaac atgtactgct tttcgacgtc gtcgccgaag accatcttga gaaactcggt
     2041 gccaggaaac ggcgtgtagg gcgtgcacac cccgacgatc ccggcgacgc gttgtggatg
     2101 caacaccccc atcgcccaca ccacaaagcc gccccaatcg tggccgacga acacggcgcg
     2161 ttcgatgtcg agcgcgtcga gtaggccgac gagatccccc gtcagctgac ccaacccgta
     2221 ggcgcggatc tcctcggggg cactcgaggc cccgtagccg cgttggtcgg gggcgatggc
     2281 gtggaacccg gcggcggcga cggcggggag ttggtggcgc cacgagtacg ccaactcagg
     2341 aaatccgtgg ctgaacacca cggcgggccc gtctccctgt tcgtgaaccg acagggtgat
     2401 ggagccatat gtcgggtctt tcaccgtgat ggggcgtgct gtgacaaagg tcatcttcga
     2461 tatttgagca ggtctgaacc ctggccgcgg acaaatcggt gtcggcgaag cggccgcggc
     2521 gccaccggcg gcactcccaa gctgatagaa gagagccatg gctgcgtcac gattcgggca
     2581 atccacctcc atcgcgggcg aacgttgggc cttgtcgctc ggggttaccg acgaggtgct
     2641 caaccttgcg gcggcgttct acatcggcga cggcatcgcg gcgggcgaat tgaaccgtcg
     2701 cttcaccctg cccctgatgg gcgaggtcga cctgtcgttg gcgctgacga tcaccggagt
     2761 cgagttcacc tcctcgcccg atcacgacgg ccgcttgcac gcgtcgattc gtgccgccgg
     2821 aagcgtcgat ttcggagcgg gcgcggccat gccggcgctg ccgggtagag cgctcgtgcg
     2881 agccgatgtg ctcgtgtccc cgcgggtcga attgcgtccc gacggtcggt tcgtggcggc
     2941 gctcgacctc gaaggcgccg agctgttgcg cacggtgatg gaggggatcg agggatctga
     3001 ggtcgacccg ggcaccgccg cggtcatggg cgacatgttg ttcgccagca tcggcggcga
     3061 catcttcagc ggacttgcgg cacagatggg gcgcatcggc atcgaactcg agccccatca
     3121 ggcccaaccg ctcgtcgatc tcggagtgcg cgccgctccc ggcgatgtgg tgatcgacga
     3181 gggccacatg accgtcgggc tcatcgcggt cgacagcgtt gaaggccacg ccatcgcacc
     3241 ccttcggccc ggccaggacg tcgtggtcgg tctggcgtcg gggtcgctga cccagttggc
     3301 ccttcgcctg gccgaggatt cgctcggggt tccgctcccc ttcgaggtcg acctgcagac
     3361 ccacagctcc gaggtcgcag cacgcatccg taaccgccgg ctcacgagtc tcgggttcct
     3421 gcccgacctg cgcaccgggg tgcgggcctc ggtcgcggcg cggctgctcg acgaccgcat
     3481 cgagctcagc ccgcgcgagg cctgggtgga actgccgctc gttccgtcgt ttgtgaaccg
     3541 gttcaaccag gcgctcggat cgcttgcctc cctgacgccg tttcaggtgt cgctgccggc
     3601 gaaggtgtcg gtgccgatcg acgactcgac gactgtcgcc gtgcgggcca ccgagttggc
     3661 gatgcgtccc gacggagtgg tgtgcgtgtt ggaggcggac ctgtgagcaa cccggagccg
     3721 cagacacctt ccgacctcga ccccgaggcg ccgtcggcgg gcgaaaccaa tcagccgatc
     3781 cctgccatcc ccggggcgtt caacctgcgg gagttcggcg gctatcgggt cgccggggga
     3841 gcgatccggc cggggttgct gtaccgaagt gacctcttga accgtgtcga tccacgtcgg
     3901 gcgcacgact ggctgggggt ggcgggcgtc gccacgctgg tcgatctgcg caccgaggcg
     3961 gaacgcggcg cggacggatt cgtcgaatcg aacaacgacc tggagtcgat ccaccggtcg
     4021 ttgctcgacg aggtgtggag ctggcgcgac gaggacgagt ccgactcgga gtggttcctg
     4081 cgagaccgca ccatctcaat gtacgagcag cattccgatc gcatcgtcgt cgtgcttgag
     4141 gacctggtcg cccgagacgg ggcggcgctg ttccactgca ccgctggcaa ggacagaacc
     4201 ggggtcgtcg ccagcgcgtt gctgggggta ctgggtgcct ccgacgaggt gatcatcgcc
     4261 gactattcca agagcaccga ggcgatggtc ggcatcgtgg gctggtaccg ggaccggatg
     4321 atcgaacaag cgcgggccca gggcctcgat gcggcgccgc ccgggcgcct cgatgaggag
     4381 gagatcattc gccgagccgc cgctccggcg accatgcgcg gcgtgatcga tgcgttgaaa
     4441 gcccgccacg gctccttcga ggggtgggcc gtcgatatcg gcgtcgaccg tcggctcatc
     4501 gatgcgctgc gggccaagtt cgttcactga ggacgattcg ccatgccgct cgctgcggcg
     4561 tcacaccaag aggacccgat gaccgaaacg cttccacaga tcgacctgtt gaacggatac
     4621 ctctatgcag ccgacccgac cccgacgtat cgatggttgc ggcatcacgc cccggtgtat
     4681 cgcgacgaca tcaaccgcct gtgggggatc agccggtacc acgacatcgt ggaggtcgag
     4741 aagcggcctc gccgatattc gaaccgcaac agctatcggc cgatcccggt gggaacctac
     4801 gacgaccgtt cgatgatcgg tcttgacgac cccgaccatc aggctcaacg tcggctcgtc
     4861 gctcgacgtt tcacgccccg ggccgtggtc gaccacgacg cggagatcac cgaggtgatc
     4921 gacggcctga tcgacgaagc gctcgccgcc gggacggtgg acgcggtcga ggcgctcgcc
     4981 gcgcccctgc cggcgatgat gatcgcgaag tacctcggct tcggcatc
//
LOCUS       DAOOMA010000125         5021 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_20109_length_5021_cov_3.545711, whole genome shotgun sequence.
ACCESSION   DAOOMA010000125 DAOOMA010000000
VERSION     DAOOMA010000125.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 5021)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5021)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5021
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20109_length_5021_cov_3.545711"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1483
                     /gene="leuA"
                     /locus_tag="PLP95_04755"
     CDS             <1..1483
                     /gene="leuA"
                     /locus_tag="PLP95_04755"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020379258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HPB45145.1"
                     /translation="PSASQTDFDFVRQLVEEDLIPDDVWIQVLVQCREELIDRTYESL
                     EGAPRAIVHFYNSTSILQRRVVFGMDREGITAIATDAARYCKKLEREYPDTVFRYEYS
                     PESYTGTEVEYAVEICNAVAEVIEPTPEHPLIMNLPATVEMYSPNIYADTIEWFLRHV
                     NNRDSIVLSLHPHNDRGCAVAAAELGVLAGADRVEGCLFGNGERTGNVDVVTLALNLF
                     SQGVDPELDISDIDALRRTAEYCNRLPVHERHPYVGDLVYTAFSGSHQDAIKKGFAQL
                     YTAEETRGDSPNTYRKWAVPYLPIDPAHVGRNYEAVIRVNSQSGKGGVAYIMEAEHGF
                     ALPRRLQVEFSKTIQSVAEDTGTEITPNAMWQQFQEMYLPDEPTIVLRSHELTSDADD
                     LTTVTAQVVVDSEPQTWKGSGNGPIAAFVDGLNHALGIELDVVDYQEHSTGRGADATA
                     VAYVETVADDNTIRWGIGRHPNISTASLLAVVSAAERVRRERR"
     gene            complement(1819..2700)
                     /locus_tag="PLP95_04760"
     CDS             complement(1819..2700)
                     /locus_tag="PLP95_04760"
                     /inference="COORDINATES: protein motif:HMM:NF016212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HPB45146.1"
                     /translation="MVNSADGPTIAEVRRLAGEALRATEFADKETVLERLAAAALELG
                     DPAGAAGARDMGGPELADGVDLVDVRRPGRPAAVRLVAPRELARRTWGTESGRVATLH
                     ALAHIEANAVNLALDAAHRFAAMPAKYYVDWTRVALEEFGHFRMLESRLAEHEIAYGD
                     LGCHDGLWDMAVSTATDARSRMALVPMVFEARGLDVTPGLIERFRRHGDDESADVLEV
                     VLADEVGHVAVGVEWFRFLCDRDGVDRSIEFERLVAEASLKLVPPFNHDARRRAGFDM
                     DDLRRWEMVFTGTQRNA"
     gene            2800..>5021
                     /gene="valS"
                     /locus_tag="PLP95_04765"
     CDS             2800..>5021
                     /gene="valS"
                     /locus_tag="PLP95_04765"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020378738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HPB45147.1"
                     /translation="MNTAPQPRPERSVPEKPGLEGLEAKWGALWEEQGTYRFDRSATR
                     DDVFSVDTPPPTVSGSLHVGHVFSYTHTDTIARFHRMCGKQVFYPMGWDDNGVPTERR
                     VENFFGVRCNATLAYDPAFTPPDKPAKDRKDFVEVSRRNFIELCERLTHDDEKVFEDL
                     WRKLGLSVDWTMTYETIGAKARLVSQKAFLDDLGRGDAYSQDAPSMWDVTFQMAVSQA
                     EVEDHERPGAYHDIVFPFSDETTEASAEFTHGDRRGVKISTTRPELLVSCVAIVCHPD
                     DERYASLIGTNVTTPLFDVEVPIVAHRLADPDKGTGAAMICTFGDNTDVIWWRELNLP
                     ARAVIERDGRFAADAPEWVTSESGRAAYGQFARKASGGARNVVVELLRDAGALVGEIR
                     QITHPVKFYEKGDKPLEIVQSRQWYLRNGGRDSDLRDLLIRRGDELDWHPPYMQARYT
                     DWVNGLNGDWLVSRQRYFGVPIPVWYRLDAEGEADYDHPLVPTADQLPVDPQIDVPAG
                     YSESQRNQPGGFRADPDILDTWATSSLTPQIACGWGTDDDLFARTFPMDLRPQGHDII
                     RTWLFSTMVRSNAQQGRAPWSNASLSGWILDPDRKKMSKSKGNVVTPAHLLDSYGSDG
                     VRYWAASGRPGTDTAFDEKEMKVGRRLAIKLLNASRFALSFGGDPLGAVNEPVDVALL
                     AELSALVAEATSAFERFDYARALERTETFFWSFCDNYLELVKIRAYGDSDASASAANA
                     "
BASE COUNT          917 a         1772 c         1542 g          790 t
ORIGIN      
        1 cccatcggcc tcccagaccg atttcgattt cgtacgccag ctcgtcgaag aggacctgat
       61 ccccgacgac gtttggattc aggtgctggt ccagtgccga gaggaactga tcgaccgcac
      121 ctacgagtcg ctcgagggcg cgcccagggc gattgtgcac ttctacaact ccacctcgat
      181 tctgcagcgc cgcgtcgtgt tcggcatgga tcgcgaaggc atcaccgcga tcgccaccga
      241 tgccgctcgt tactgcaaaa agctcgagcg cgaatacccc gacacggtgt ttcgctacga
      301 gtactcgccg gagtcctaca cgggaaccga ggtcgagtac gcggtcgaga tctgcaacgc
      361 ggtggccgag gtgatcgaac cgacccccga gcacccgctc atcatgaacc tgccggcgac
      421 ggtcgagatg tactcgccga acatctacgc cgacacgatc gagtggttcc tgcgtcacgt
      481 caacaaccgc gactccatcg tgttgtccct gcatccccac aacgaccgcg gctgtgcggt
      541 ggcggcggcc gaactcggcg tcctggcggg tgccgaccgc gtggaggggt gcctgttcgg
      601 caatggcgaa cgaaccggca acgtcgacgt cgtgaccttg gcgctcaatc tgttcagtca
      661 gggggtcgac cccgaactcg acatctccga catcgatgcg ttgcgccgca ccgccgaata
      721 ctgcaaccgc ctgccggtcc acgaacgcca cccctacgtc ggcgatctgg tgtacaccgc
      781 gttttcgggc tcgcaccagg acgccatcaa gaaagggttc gcccagctct acaccgccga
      841 ggaaacccgc ggcgactcgc ccaacaccta ccgcaagtgg gccgtgccgt atctgccgat
      901 cgacccggcc cacgtcgggc gcaactacga ggcggtcatc cgggtgaaca gccagtccgg
      961 caagggcggc gtcgcctaca tcatggaagc cgaacacgga ttcgcgttgc cgcgacggct
     1021 gcaggtcgag ttctccaaga ccattcagtc ggtcgccgag gacaccggaa ccgagatcac
     1081 cccgaacgcc atgtggcagc agttccagga gatgtatctc ccggatgagc cgacgatcgt
     1141 gttgcgcagc cacgaactga ccagcgacgc cgatgatctc accacggtga ccgcccaggt
     1201 cgtcgtggat agtgaaccgc agacgtggaa gggcagcggc aacggtccga tcgccgcgtt
     1261 cgtcgacggg ctcaatcacg cgctcggcat cgaactcgac gtcgtcgact accaggagca
     1321 ttcgaccggg cgcggagccg atgccaccgc ggtcgcgtac gtcgagaccg tcgccgacga
     1381 caacacgatc cgttggggaa tcggccgaca ccccaacatc agcacggcct cgctgctcgc
     1441 cgtggtgtcc gccgccgagc gagttcgacg cgagcgccgc taggcgctcg cgctgcgcgg
     1501 cctggccgca cggcctggcg agccacaggt gcacgcgtac gcgaaagggg ccgagtcgga
     1561 tgactcggcc cctttcgcgt cggacgatac gcccgactcg ctgtgtcagg tcaactactt
     1621 gaaggtgcag ttgacgttga gtgggccttc aaggatcgtg acgatgcacg ggccgaccgg
     1681 aatcttgcac gcgctcgcgg cgaacggtat cgccatgatc attgaggcaa ggatgaccct
     1741 gctggtacga cgcataattg tttccccccc ctgtagttgt caggacaccc gctactacag
     1801 tccaccgttc gcgccgcgtc aagcatttcg ctgcgttccg gtgaatacca tctcccaacg
     1861 ccgcaagtcg tccatgtcga agcccgcccg acgccgggca tcgtggttga acggcggcac
     1921 cagtttcagc gaggcctcgg ccacgagacg ctcgaattcg atcgaccggt cgacgccatc
     1981 tcgatcacac aggaaccgga accactccac cccgaccgcc acatggccga cctcgtcggc
     2041 caggaccacc tcgaggacgt ccgcgctctc gtcgtcaccg tggcgacgga accgctcgat
     2101 gagcccggga gtgacgtcga gaccccgtgc ctcaaacacc atcggaacca gggccatacg
     2161 cgaacgcgcg tcggtggcgg tgctcaccgc catgtcccac aggccgtcgt ggcatccgag
     2221 gtcgccgtag gcgatctcgt gttccgccaa cctggattcg agcatccgga agtgaccgaa
     2281 ctcctcgagc gcgacccgtg tccagtcgac gtagtacttc gccggcatcg ccgcgaaacg
     2341 atgcgccgca tcgagcgcga gattcacggc attggcctcg atgtgggcca acgcgtgcag
     2401 cgtggcaacc cgccccgact cggtccccca ggtccgcctc gccaactccc gcggggcgac
     2461 gagcctcacc gccgccggcc gccccggacg gcggacatcc accagatcga caccgtccgc
     2521 gagctcggga ccgcccatgt cacgggcccc ggcggccccg gcgggatcgc cgagctccaa
     2581 cgccgcggcg gccaagcgct cgagcaccgt ctccttgtcg gcgaactccg ttgcccgcaa
     2641 tgcctcgccc gccaaccgac ggacctcggc gatcgtcggg ccgtccgcgc tgttgaccat
     2701 cgcaccgttc taccccatcg acggagccga cacgtgggtc ggcccgggcc cgcaacccct
     2761 caggcccacc tggcgacaca cggtacgatt ggcgcaacta tgaataccgc accacagccc
     2821 cgccccgaac gcagcgtccc cgaaaaaccg gggctcgaag ggctcgaggc caagtggggt
     2881 gcgctgtggg aggaacaggg cacctaccgt ttcgatcgct ccgcgacccg tgacgacgtc
     2941 ttttccgtcg acaccccgcc gccgaccgtg tcgggctccc tgcacgtcgg ccacgtgttt
     3001 tcctacaccc acaccgacac catcgcccgc ttccaccgca tgtgcggcaa gcaggtgttc
     3061 tatccgatgg gctgggacga taacggggtg cccaccgagc gacgggtcga gaacttcttc
     3121 ggggtccgct gcaatgcgac cctcgcctat gaccccgcct tcaccccacc ggacaagccc
     3181 gccaaggacc gcaaggactt cgtggaggtc agccgacgca acttcatcga actgtgcgag
     3241 cggctcaccc acgacgacga gaaggtcttc gaggacctgt ggcgaaagct cggtctgtcg
     3301 gtcgactgga ccatgaccta tgagacgatc ggggccaagg cccgcctcgt cagccagaag
     3361 gccttcctcg acgacctcgg ccgcggcgat gcctacagcc aggatgctcc ctcgatgtgg
     3421 gacgtgacgt tccagatggc ggtgagtcag gccgaggtgg aggaccatga acgccccggc
     3481 gcgtatcacg acatcgtgtt cccgttcagc gacgagacga ccgaagcctc cgccgagttc
     3541 acccacggcg accgtcgggg cgtgaagatc tcgacgactc gacccgaact gctggtgagt
     3601 tgtgtggcga tcgtgtgtca ccccgacgac gaacgctatg cgagcctcat cggaacgaac
     3661 gtgaccaccc cgctgttcga cgtcgaggtg ccgatcgtcg ctcaccgtct cgccgacccc
     3721 gacaagggca ccggcgcggc gatgatctgc acgttcggcg acaacaccga cgtcatctgg
     3781 tggcgcgagt tgaaccttcc ggcgcgggcc gtcatcgagc gcgacggccg tttcgcggcg
     3841 gacgcgcccg agtgggtcac ctccgagtcc ggacgagcgg cgtacggaca gttcgcgcga
     3901 aaggcctcgg gcggggcgcg caacgtggtg gtcgagttgt tgcgcgacgc cggcgcgctc
     3961 gtcggcgaga tccgacagat cacccacccg gtcaagttct acgaaaaggg cgacaaacca
     4021 ctcgagatcg tccagtcgcg gcagtggtac ctgcgcaacg gcggtcgaga ttccgacctg
     4081 cgcgacctgc tgatccgccg cggcgacgaa ctcgactggc atccgcccta catgcaggcc
     4141 cgctacaccg actgggtgaa cgggctcaac ggcgactggc tggtgagccg ccagcggtac
     4201 ttcggcgtgc cgatccccgt gtggtaccgc ctcgacgccg agggcgaggc cgactacgac
     4261 cacccgctcg tgccgaccgc cgatcaactg ccggtcgatc cgcaaatcga cgtgccggcc
     4321 ggctacagcg aatcccaacg caaccaaccg ggcgggttcc gggccgatcc cgacatcctc
     4381 gacacgtggg cgacctcctc gctcaccccg cagatcgcat gtggctgggg aaccgacgac
     4441 gacctgttcg ctcgcacgtt tccgatggat cttcgcccgc agggccacga catcatccgg
     4501 acgtggctgt tttccacgat ggtgcgttcg aatgcccagc agggtcgcgc cccgtggtcg
     4561 aacgcatcgt tgtcggggtg gatcctcgac cccgaccgca agaagatgtc gaagtcgaag
     4621 ggcaacgtcg tcaccccggc gcacctgctc gacagctacg gctccgacgg ggttcggtac
     4681 tgggcggcat cgggtcggcc cggcaccgac acggccttcg acgaaaagga aatgaaggtc
     4741 ggccgtcgcc tggccatcaa gttgctcaac gccagccggt tcgcgctcag cttcggtggc
     4801 gacccgctcg gggcagtgaa cgaacccgtc gacgtcgcac tcctcgccga actgtccgcg
     4861 ctcgtcgctg aggccacctc cgcgttcgaa cgcttcgact atgcccgtgc gctcgaacgc
     4921 accgaaacgt tcttttggag cttctgcgac aactatctcg aactcgtgaa gatccgggcc
     4981 tacggcgaca gcgacgcatc cgcctcggcg gcgaacgcgt t
//
LOCUS       DAOOMA010000126         5006 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_20192_length_5006_cov_3.798627, whole genome shotgun sequence.
ACCESSION   DAOOMA010000126 DAOOMA010000000
VERSION     DAOOMA010000126.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 5006)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5006)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5006
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20192_length_5006_cov_3.798627"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1033)
                     /locus_tag="PLP95_04770"
     CDS             complement(<1..1033)
                     /locus_tag="PLP95_04770"
                     /inference="COORDINATES: protein motif:HMM:NF014790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HPB45148.1"
                     /translation="MDFGDSSAEAQFRQRFRDWLADNNPGLPTSSTDDEYWAGMAAWH
                     QSLHGAGFFGMSWPKSVGGQDLASVYDVIIDDELAAAGAPPRPSLGYLVQGFLEHGSP
                     EVQQRFLPGIVDGTQRWCQGFSEPDAGSDLASLRTRADLDGDEYVITGHKVWTSYSDD
                     ADWCLVLARTNHEVPKHKGISAFAVPMHQSGIEQRPLKMINGITKEFGEVIFDEARVP
                     AANMIGDPGEGWHMAMTVVSHEREPGELGFVARYRKLVKDLTKRVATEGGHSEQVRDL
                     GWAIVESEMLRLHVSRRLSDRLDGIVTGVEAPTLPLLSPREEAHADLWATGIAADAEA
                     TRFVREHLDS"
     gene            complement(1020..2123)
                     /locus_tag="PLP95_04775"
     CDS             complement(1020..2123)
                     /locus_tag="PLP95_04775"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HPB45149.1"
                     /translation="MDVRLSSEQHALRDAAARIVDRHGVSAVGQLDDTERRVKLRDAI
                     VESGWLELRTPDDDGSALASAVEAAIVAEELGRGLADAAYLGPTLAVELRRLAGAPPA
                     AGPETVAMTSDLSRLAESGSTAGSRALAIDSDGAESALAVTSHGMTTSLVSLPVAPFN
                     DPDAQVDLTRSCAAVHGGDLDGEATLDGGDVDGGDLDGDDLTRWTALALALTSADLVG
                     VMRGAIDLSVDYAKERRQYGSPIGSFQAVQHLLADAFVAMEGSRSVALHAAWAVDALS
                     PSEALAAGALAKAYAARAARNVCEIAVQVHGGIGNTWECLAHVYLRRALVSTDIAGGA
                     GINLDRVLASRGVGADVRPVQQLQPTGAGHGLR"
     gene            complement(2123..3778)
                     /locus_tag="PLP95_04780"
     CDS             complement(2123..3778)
                     /locus_tag="PLP95_04780"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate-forming enzyme family protein"
                     /protein_id="HPB45150.1"
                     /translation="MSTSILDRSLTRAAQRFGDRVAVRAAEHSWSFEALDTAVSDAAA
                     ALGRRGAQPGARVAVMMANRVEFLTSVLGIVRAGGVAVLISPAWKSTEVANAVALTAP
                     VLGIGDDDSIGVLCAQLGAERVVNVTDGEFGSGQSETEAVAAQRADAAADADADVDID
                     ADADADADADADAVLVFSSGTTGMPKAVRHTHRSLGHATRHWVEALGLGPDDRFQVAT
                     PPSHILGLLNLLAAIESGATVRLHRRFDLDEVLACIEAERMTLEMAVAPIALALANHP
                     NLEAYDLSSLRYIMWGATPVTPSVAETVTRRSGVRWLAAYGASELPVLACNPVDRPDQ
                     WRLDSAGLAPRGVELRVVDLDGGEVLASGEVGEIQARGESLMAGYLPAEATDEVFVDG
                     WYRTGDVGWIEPDGWVHLTDRSKEMIKVSGFQVAPAEIEAVLLSHDAVVDCAVFGVAD
                     PVAGEVPAAAVQLAAEAVIREGELEGLVADELATYKRLRHVVVVDEVPRLPSGKVLRR
                     VLRDRFAEPVGETLAGETLAGSKAGSKSGSNAGSNAAEAEQEE"
     gene            3842..>5006
                     /locus_tag="PLP95_04785"
     CDS             3842..>5006
                     /locus_tag="PLP95_04785"
                     /inference="COORDINATES: protein motif:HMM:NF012656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HPB45151.1"
                     /translation="MSSPEGSTPTGATDPEVRREIVETVRRFVAKEVIPVAGDLEHAD
                     EFPADIVAKMRDLGLFGVTIPESLGGLGLDLLTYVAIIEELAYGWMSLTGIINTHTMC
                     ATLIMYHGNEEQQRRILPTMASGERRGALSLSEPDAGSDTRNISCKATRDGDEFVLNG
                     TKAWVTNGERASVVALAARTEAGISTFVVEKDPGPVFEGVSTSKNVGKLGYKGVETVE
                     MAYVDHRIPAANLIGTDGRGLPQILSVLEVGRINIASRAVGVARAAFDAALSYSQQRS
                     TFGKPIAEHQAIQLKIADMATRLEAARLLTQNAANRKAAGERCDVEAGMAKLFASETA
                     LELSMEAMRIHGGVGYTTEFPVERYYRDAPLMVIGEGTNEIQRLVIARGLLARA"
BASE COUNT          852 a         1799 c         1590 g          765 t
ORIGIN      
        1 ggctgtcgag gtgctcgcgc acgaagcggg tggcttcggc gtcggcggcg atgccggtgg
       61 cccacaggtc ggcgtgggcc tcctcccggg gcgagagcag gggcagggtg ggggcctcga
      121 cgccggtgac gatgccgtcg aggcggtcgg acaggcgacg actcacgtgc aggcgcagca
      181 tctccgattc gacgatggcc cagccgaggt cccgcacctg ttcggagtga ccgccctcgg
      241 tggcgacgcg tttggtgagg tccttgacga gcttgcggta tcgggcgacg aagcccaact
      301 cgccgggctc gcgctcgtgg ctcaccacgg tcatggccat gtgccacccc tcgcccgggt
      361 cgccgatcat gttcgccgcc ggcacgcggg cctcgtcgaa gatgacctcg ccgaactcct
      421 tcgtgatgcc gttgatcatc ttgagcggac gctgctcgat gccgctctga tgcatcggca
      481 ctgcgaatgc cgaaatgccc ttgtgtttgg ggacctcgtg gttggtgcgg gccaacacga
      541 ggcaccagtc ggcgtcgtcg gagtagctcg tccacacctt gtggccagtg atgacgtatt
      601 cgtcgccgtc gaggtcggct cgggtgcgca gcgaggcgag atcggacccg gcgtcgggct
      661 cgctgaaccc ctgacaccac cgctgggtcc cgtcgacgat gcccggcagg aagcgctgtt
      721 gcacctcggg ggaaccgtgc tcgagaaacc cctgcacgag atagccgagg ctcggtcgag
      781 ggggcgcccc ggccgcggcg agttcgtcgt cgatgatgac gtcgtacacg ctcgcaaggt
      841 cctgaccgcc caccgatttc ggccacgaca tcccaaagaa cccggccccg tgcagggact
      901 gatgccacgc cgccatgccg gcccagtact cgtcgtcggt cgacgaggtc ggcagacccg
      961 ggttgttgtc ggccagccag tcgcggaagc gttggcggaa ctgcgcctcg gcgctcgaat
     1021 caccgaagtc catggcctgc tcctgtcggt tgaagctgtt gaaccggtcg cacatcggcc
     1081 cccacgcctc gcgaggccag cacccggtcg aggttgatgc ccgctccccc ggcgatgtcg
     1141 gtcgaaacca acgcccggcg caggtagacg tgagcgaggc actcccacgt gttgccgatg
     1201 cctccgtgga cctgcaccgc gatctcacac acgttgcgag cggcgcgcgc ggcgtacgcc
     1261 ttggcgagcg cgccggcggc gagcgcctcg gaggggctta gcgcgtcgac agcccaagcc
     1321 gcgtggaggg cgacgctgcg cgagccttcc atcgccacga aggcgtcggc caacaggtgt
     1381 tgcaccgctt gaaacgaacc gatgggcgac ccgtattggc ggcgctcctt ggcgtagtcc
     1441 accgacagat cgatggctcc gcgcatgacc ccgacgagat cggcactcgt gagcgctagc
     1501 gcgagggcgg tccaacgggt gaggtcgtcg ccgtccagat ccccgccgtc cacatccccg
     1561 ccgtccaggg tcgcttcgcc gtcgagatcc ccgccatgga ccgcagcgca cgatcgcgtg
     1621 agatcgacct gcgcgtcggg gtcgttgaac ggagcaaccg gaagcgacac caacgatgtc
     1681 gtcatcccgt gcgaggtcac cgccaacgcc gactccgccc cgtccgagtc gatcgccagt
     1741 gcccgcgaac cggccgtgga ccccgactcg gccagacgcg acaagtccga ggtcatcgcc
     1801 acggtctcgg gccccgccgc cggcggggca ccggcaagtc gccgcaactc gaccgccagg
     1861 gtcggaccga gataagcggc atcggccaac ccacggccca gttcctccgc cacgatcgcc
     1921 gcttccaccg ccgaggccaa cgccgacccg tcgtcatcgg gcgtgcgcaa ctccagccac
     1981 cccgactcga cgatcgcgtc gcgcaacttg acgcgccgct cggtgtcgtc gagttggccc
     2041 acggcgctca ccccatgacg atccacgatc cgcgccgcgg cgtcacgcaa cgcgtgttgt
     2101 tccgacgaga gtcgaacgtc catcactcct cctgttctgc ctcggcggca ttcgaaccag
     2161 cattcgaacc agatttcgag ccagctttcg agccggccag cgtttcgcca gccagcgttt
     2221 cgccgaccgg ttcggcgaat cgatcgcgta gcacgcggcg aagcaccttg cccgagggca
     2281 gtctcggaac ctcgtccacg accacgacat gacgcaatcg cttgtaggtg gcgagctcat
     2341 cggcgacgag cccttcgagt tcgccctcgc ggataacggc ttcggcggcg agttgcaccg
     2401 cggcggccgg gacctcgccg gcgacgggat cggcaacgcc gaataccgcg caatcgacga
     2461 cggcatcgtg tgacaacaac accgcctcaa tctcggcagg tgcgacctga aacccgctca
     2521 ccttgatcat ctccttcgac cgatcggtga gatgtaccca cccgtcgggt tcgatccatc
     2581 cgacgtcacc ggtgcggtac cacccatcca cgaacacctc gtcggtcgcc tccgccggca
     2641 gataccccgc catcaacgac tcgccgcggg cctgaatctc ccccacctcg ccggaagcaa
     2701 gcacctcacc gccgtcgagg tcgaccacgc gcaattcgac gccgcgtggg gcgagtcctg
     2761 cggagtcgag acgccactgg tcgggtcgat cgaccggatt gcaggccagc acgggcaact
     2821 cgctggctcc ataggccgct agccatcgca cgccgcttcg ccgggtcacg gtctcggcga
     2881 cactcggggt gacgggagtc gctccccaca tgatgtagcg cagcgaggac aggtcgtagg
     2941 cctcgaggtt cgggtgattg gcgagggcca gggcgatcgg cgcgaccgcc atctccaggg
     3001 tcatgcgctc ggcctcgata caggccagca cctcgtcgag atcgaagcgg cggtgcaggc
     3061 gaaccgtcgc accggattcg atcgccgcga ggagattgag cagcccgagg atgtgcgacg
     3121 gcggggtcgc gacctggaac cgatcgtcgg ggcccagccc caacgcctcg acccagtgtc
     3181 gtgttgcgtg gcccaacgaa cgatgggtgt ggcgcaccgc cttgggcatg ccggtcgtgc
     3241 ccgaactgaa caccagcacc gcgtcggcgt cggcgtcggc gtcggcgtcg gcgtcggcgt
     3301 cgatgtctac gtcggcgtcg gcgtcagctg cggcgtcagc gcgctgggcc gcaacggctt
     3361 ctgtttcgct ctggccggac ccgaactcgc catcggtgac gttcacgacc cgctcggcgc
     3421 ccaactgcgc gcagagcacg cctatcgagt cgtcgtctcc gatgcccagc accggtgccg
     3481 tcagcgcgac cgcattggcg acttcggtgc ttttccatgc cgggctgatg aggaccgcaa
     3541 cgcccccggc gcgaacgatg ccgagcaccg aggtgaggaa ctcgacgcgg ttggccatca
     3601 tgaccgcgac ccgggcaccc ggctgggctc cacgtcggcc caacgccgca gcagcgtcgc
     3661 tcacggcggt gtcgagcgcc tcgaaggacc aggagtgctc cgccgcgcga acggcgacgc
     3721 ggtcgccgaa ccgttgcgca gcccgagtca gactgcgatc caatattgac gtcgacatcg
     3781 gaaacatcat tctcgctttt tgagaggcac gtcaaccccc acggtcggta ccattctcgc
     3841 catgagcagc cccgaaggtt ccacccccac cggagcgacc gatcccgagg ttcgccgcga
     3901 gatcgtcgag accgtccgcc gtttcgtcgc caaggaggtc atcccggtcg ccggcgacct
     3961 cgaacacgcc gacgagtttc ccgccgacat cgtggccaag atgcgcgacc tcggcctgtt
     4021 cggcgtcacc atccccgaat cgctcggtgg cctcggcctc gatctgttga cctatgtcgc
     4081 catcatcgaa gaactcgcct acggctggat gtcgctcacc ggcatcatca acacccacac
     4141 gatgtgcgcc acgctcatca tgtatcacgg caatgaagaa cagcagcgcc gcatcctgcc
     4201 gacgatggcc tctggcgaac ggcgtggagc gctgtcgctg tccgaacccg acgccggcag
     4261 cgacacccgc aacatctcct gcaaggcgac ccgcgacggc gacgagttcg tcctcaacgg
     4321 aaccaaggcg tgggtgacca acggcgaacg ggcatcggtg gtcgccttgg ccgcacgcac
     4381 cgaggccggc atctcgacct tcgtcgtcga aaaggacccg gggccggtct tcgaaggggt
     4441 ctccacatcc aagaacgtcg gcaaactcgg ctacaagggc gtcgaaaccg tcgagatggc
     4501 ctacgtcgat caccgcatcc cggccgccaa cctgatcggc accgacggcc gcggcctgcc
     4561 ccagatcctc tcggtgctcg aggtgggccg catcaacatc gcgtcgcggg ccgtcggcgt
     4621 ggcacgggcc gcgttcgacg ctgccctcag ctactcccaa cagcgatcca ccttcggcaa
     4681 accgatcgcc gaacatcagg ccatccaact caagattgcc gacatggcca cccggctcga
     4741 agccgcacgc ctcctcaccc aaaatgccgc caaccgaaag gccgccgggg aacgctgcga
     4801 cgtcgaagcc ggcatggcaa agctgttcgc cagcgagacc gccctcgaac tgtcgatgga
     4861 ggccatgcgc atccacggcg gtgtcggcta caccaccgag ttccccgtcg agcgctacta
     4921 ccgcgacgcg cccctcatgg tcatcggcga gggcaccaac gaaatccaac gactcgtcat
     4981 cgcccgcggc ctgctcgcac gcgctc
//
LOCUS       DAOOMA010000127         4987 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_20290_length_4987_cov_4.565085, whole genome shotgun sequence.
ACCESSION   DAOOMA010000127 DAOOMA010000000
VERSION     DAOOMA010000127.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 4987)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4987)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4987
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20290_length_4987_cov_4.565085"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(128..1795)
                     /locus_tag="PLP95_04790"
     CDS             complement(128..1795)
                     /locus_tag="PLP95_04790"
                     /EC_number="1.8.7.1"
                     /inference="COORDINATES: protein motif:HMM:NF013259.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0050311 - sulfite reductase (ferredoxin)
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite/sulfite reductase"
                     /protein_id="HPB45152.1"
                     /translation="MASINIPAAKRAGLPVDLERLRAEGDSWLTAEDRFALKTYGACT
                     QLQDGVFMVRVRIAGGVLPTTHARALARIANRHAEDWLHLTTRQSCELHWVEDRRVPD
                     VLDELARAGLSTRSACGHTVRNVMCSEDAGVGLDEPFDCFPDARLISDTLVARSATLN
                     VTLPGRINISLGGSPRCRHDALVNDVGLVSIVRDGEAGYEIWAGGSLGKAPSLGVMLA
                     PFVPRRQLPAAIDAIVDLYVAHGDFDTPAKGRMKFVVERLGVDGFRGAYLEAFREAQL
                     RPQPTVPPVEVLSDEATTVILAETPPGGWSIGVRPQRSAGLATVTIDLPLGDTNSSEF
                     RLLCDLADTYADGHLTFTRDQNIALRNVSLAGVAAIRNAIAQRGLFLLGEAPNAQIRA
                     CTGATVCALGITDSPGLGKLLSTSASLRRNSALSVFISGCPNSCAQHQVSDIGLAGSK
                     VRVNGRTSDGYQVFVGAVLDDHLIGEVVGRVAERDLPTALDAIVGTWEALRHPNESLG
                     RTVRRLGLDAFSLQIAAVLEDRWAPGPEEVNTATIEPDLTTTAVSLP"
     gene            1949..3226
                     /locus_tag="PLP95_04795"
     CDS             1949..3226
                     /locus_tag="PLP95_04795"
                     /inference="COORDINATES: protein motif:HMM:NF012861.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HPB45153.1"
                     /translation="MLSTVRFSHPAREVILGVFTRRRLAGLVVAAGLVGGLIGAAYLF
                     VLELVSERLLPEHWSAAGHLGVMIVVGVVVTGLIRLLGPPSDVELLVDNIHVSGGAEG
                     VRSLRSLIPVSLICVGAGGPLGPEAPLVTATGTMASWMGGRAGLDREPLRVLSITGMA
                     AGFTVLFGAPLGAAVFALEILHRRGLEYYEALLPASVGSLSGYAVSIVLSGAGLEPIW
                     SFPSPERLVSGDLAIAVAAGVVGALIGYLFTYLTVAMRRLAAWVAPGARPVVGAVVLS
                     GLAALSPYALTNGEVQMQHLSATTVTVGALLVGAAAKLVASAVAMATGWRGGFIIPLF
                     FVGFCLGRALDGHLPFGNSWVLVAALMVAANVAVTKTPIGSTLVVTEMAGMVLLPTVL
                     IAAIVALLLSSPVSLIESQQRRFDPSKPGEGDT"
     gene            3223..3660
                     /locus_tag="PLP95_04800"
     CDS             3223..3660
                     /locus_tag="PLP95_04800"
                     /inference="COORDINATES: protein motif:HMM:NF024854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HPB45154.1"
                     /translation="MTNRNSRDDGLRDEEYRALARFRHALRTFESFSSNAAKTLGLSP
                     AQHQLLLAIRGHEAPPPSISDIAERLLIRVHSATELVARADANGLVRRLTDPADGRRI
                     ILELTDQGERVLAALSVVHRDELRRFRQEMNDVLGELEPASGI"
     gene            complement(3725..>4987)
                     /locus_tag="PLP95_04805"
     CDS             complement(3725..>4987)
                     /locus_tag="PLP95_04805"
                     /inference="COORDINATES: protein motif:HMM:NF014581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /protein_id="HPB45155.1"
                     /translation="PGCGKSSTLNTVLSGYNGPVVATTTKTGEWEQSYVTIRAERGPI
                     CLFDPANKVADPHPSARRIRFDVVQLCTSYAEARRIAWQIAEGADRNSLTAADFWISY
                     AQSLLGPLLFAANLDSGGIADVLSWNVADRLDRPIEILTDHGHEPEAAQVRAIVETDS
                     RTRDSVLATVTALLAPFSTGDAARLSTQGHRFDPFADWIEPSGTVLLCLDPTDTAQLG
                     LLIEIFLSWLIRARTTLADNGWSGPSCLLGGDEIAHVCALSTLPRLIGEGPGRGVSAI
                     VCLQDLSQAHARWGASGDLFVTATGNRVLFPGVTSTALLNWVTPLLGRGTSRPAGFVN
                     RNRSGFDLDDDHEPSDVENVGQVIHPGRNAAVLITPDSSVYSAQQARAQDDPLYCQAI
                     AESQATLISWSILPPAPDEREPPAGSDT"
BASE COUNT          771 a         1545 c         1767 g          904 t
ORIGIN      
        1 gcctggggga tggcgttggg catgatcgct cctggggctt cctgtccggg gggttgaacg
       61 attcgaaggt gggtgagggt tgaaggtact ggaggctggg gtggggtcgt cgggctcgcg
      121 gcggcgctca ggggagactg accgcagtgg tggtgaggtc gggttcgatc gttgcggtgt
      181 tcacctcctc aggtccgggt gcccatcggt cctcgagcac ggcggcgatt tgaagtgaga
      241 aggcatcgag gcccaggcga cgtacggttc gaccgagcga ttcgttcggg tggcgcaggg
      301 cctcccaggt tccgacgatg gcgtcgagtg cggtggggag gtcgcgttcg gccacgcgcc
      361 cgaccacttc accgatgaga tggtcgtcga gcaccgctcc gacaaatacc tggtagccgt
      421 cgctggtgcg cccgttcaca cgcactttcg atccggcgag tccgatgtcg ctgacctggt
      481 gttgggcaca ggagttcggg cagccggaaa taaacaccga gagtgctgaa ttgcggcgca
      541 acgatgcgct ggtcgacagc agcttgccca acccggggga gtcggtgatg cccagtgcgc
      601 acacagtcgc cccggtgcag gcgcggattt gggcattcgg cgcctcgccg agcaggaaca
      661 ggccgcgctg ggcgatggcg tttcggatgg cggcgactcc cgccaggctc acgtttcgaa
      721 gtgcgatgtt ctggtctcgg gtgaaggtga ggtggccatc cgcgtaggtg tcggcgaggt
      781 cgcagagcag tcgaaactcc gaggagttcg tgtcgccgag gggcaagtcg atggtgacgg
      841 tggccagccc tgcgctgcgc tgagggcgta cgccgatgct ccaacctccg ggaggcgtct
      901 cggccagaat cacggtcgtg gcctcgtcgg aaagcacttc gacgggtggg accgtgggtt
      961 gtggtcgcag ctgtgcttca cggaacgctt cgaggtaggc gccgcggaat ccatcgactc
     1021 cgaggcgttc gaccacgaac ttcatccgcc ctttggccgg ggtgtcgaag tcgccgtggg
     1081 caacgtagag atcgacgatc gcatcgatgg ctgccggaag ctgtcgtcga ggcacgaagg
     1141 gcgccaacat cacgccgaga ctcggggcct tgccgaggct gccgcccgcc cagatttcgt
     1201 atcccgcctc gccgtcgcgc acgatcgaca cgagaccgac gtcgttgacg agcgcatcgt
     1261 ggcggcatcg tggcgagccg cccagggaga tgttgatgcg ccccgggagg gtcacgttca
     1321 gggtcgccga gcgtgcgacg agggtgtcgg agatcaaccg ggcatcgggg aagcagtcga
     1381 agggttcgtc gaggccgacc ccagcgtctt ctgaacacat gacgttgcgc acggtgtggc
     1441 cgcaagcgga gcgggtggag agtccggcgc gggcgagttc gtccaggacg tcgggcaccc
     1501 gtcggtcctc gacccagtgc agttcgcaac tctggcgggt cgtgaggtgc aaccagtcct
     1561 cggcatgacg gttcgcgatg cgagccagcg cccgggcatg ggtggtcggc agtaccccgc
     1621 cggcgatgcg gacccgcacc atgaacacgc cgtcttgaag ctgggtgcac gccccgtagg
     1681 tcttgagggc gaagcggtcc tctgcggtga gccacgaatc gccctcggcc cgaaggcgtt
     1741 cgaggtcgac cggaaggccg gcacgctttg ctgcggggat gttgatcgaa gccatgcgat
     1801 gaccgtacgg aggcgatgtt tcgcaaccgt tctcccggcg tgacggttcc ctcaccgtgc
     1861 cgcatcgccg cctgtgagga gttgggggag cgatctggtc cccatcggag ttcgctccaa
     1921 ctaatacatc gtgatacgat ctaatcccgt gctcagtacc gtacgattca gtcatccggc
     1981 ccgcgaggtc atcctcgggg tcttcacccg tcgacgactc gccgggctcg tcgtggcggc
     2041 aggcctcgtt ggtggactca tcggagccgc ctacctcttc gtactcgagc tggtctccga
     2101 acggctgttg cccgagcact ggagcgcggc cgggcacctg ggcgtgatga tcgtcgtcgg
     2161 cgtggtggtg acggggttga ttcggctgct cggtccgccg agcgacgtcg aactactcgt
     2221 cgacaacatc cacgtttcgg gaggcgccga aggagtgcga agcctccgat cgctcatccc
     2281 cgtgtcgttg atctgcgtcg gagcgggcgg tccgctcggc ccagaagcac cactggtcac
     2341 cgcgaccgga acgatggcgt cctggatggg tggccgggcg gggctcgatc gggagccgtt
     2401 gcgcgtgttg tccatcaccg gtatggcggc cgggttcacg gtgctgttcg gcgctccact
     2461 cggtgcggcc gtattcgccc tcgagatcct gcatcgtcgc ggcctggagt attacgaggc
     2521 gctcttgccg gcctcggtcg ggtcgttgtc gggctatgcc gtgtcgatcg tgttgtcggg
     2581 ggccggactc gaaccgatct ggtcatttcc cagccccgag cgactcgtgt cgggcgacct
     2641 ggccatcgcc gtcgctgccg gcgtcgtcgg agcgctcatc ggatacctgt tcacctacct
     2701 gacggtggcg atgcggcgat tggcggcgtg ggtggcgccg ggagcgcgtc ccgtggtggg
     2761 tgccgtggtg ttgtccgggc tggcggcgct gtcgccgtat gcgctcacca acggcgaggt
     2821 ccagatgcaa cacctcagcg ccacgaccgt caccgtcggg gcgctactgg tgggggcggc
     2881 ggcgaagctc gtggcgagcg cggttgcgat ggccaccgga tggcgtggcg ggttcatcat
     2941 cccgctgttc ttcgtcgggt tctgtttggg acgtgcgctc gatggccacc ttccgttcgg
     3001 aaactcctgg gtcctcgtgg cggcgctgat ggtggccgcc aacgtcgccg tcacgaagac
     3061 gcccatcggc tcgacccttg tggtcaccga gatggccggc atggtgctgt tgccgaccgt
     3121 actgatcgca gcgatcgtgg cgctgttgtt gtcctcgccg gtttcactga ttgaatctca
     3181 gcagcgacgc ttcgatccgt cgaagccggg ggagggcgac acatgaccaa tcgcaactcg
     3241 cgcgacgatg gcctgcgcga cgaggagtac cgggcgttgg cgcggtttcg ccatgcgctt
     3301 cgcaccttcg aatcgttctc gagcaatgcc gcgaagaccc tcgggctttc gccggcacaa
     3361 caccagttgc tgctcgccat tcgcggccat gaggccccac ccccgtcgat ctccgatatc
     3421 gccgaacgcc tgctcatccg ggttcactcg gcgaccgagc tcgtggcccg ggccgatgcg
     3481 aacgggctcg tgcgccgtct caccgacccc gccgatgggc gacgcatcat tctcgaactc
     3541 accgaccagg gagagcgggt gctcgcggcg ctgtcggtgg tgcatcgcga cgaactgcgc
     3601 cggttccgcc aggagatgaa cgacgtgctc ggagagctcg aaccggccag cggcatctga
     3661 cctcgggcga ccgaccaccg gcgaccgacg tcggcatcgg gcgaacttcc tgccggggct
     3721 gacatcacgt gtcgcttccg gcaggaggtt cgcgttcgtc tggcgccggc gggaggatgc
     3781 tccacgagat caacgtggcc tgcgattcgg caatggcctg gcagtacaac gggtcgtctt
     3841 gggcccgggc ctgttgtgcg gaatacaccg acgagtcggg ggtgatcagc accgctgcgt
     3901 tccggccggg atgaatgacc tgtccgacgt tttcgacgtc gctcggctcg tgatcgtcgt
     3961 cgagatcgaa acctgaacgg ttccggttga cgaagcccgc aggcctcgac gtgccgcgcc
     4021 ccagcagcgg ggtcacccag ttgagcagcg ccgtgctggt gacgccggga aacaacacgc
     4081 ggttgccggt cgcggtgaca aacagatctc ccgacgcacc ccaacgtgcg tgcgcctgac
     4141 tcaaatcctg gagacacacg atggcggaca cgccgcggcc aggcccctcg ccgatgagac
     4201 gcggcagggt gctgagcgcg cacacgtggg cgatctcgtc gccaccgagg agacacgacg
     4261 gtccgctcca accgttgtcg gcgagtgtag ttcttgcacg gatcagccag ctcaggaaga
     4321 tctcgatcag cagcccgagc tgagcggtgt cggttgggtc gaggcacaac agcaccgtgc
     4381 cgctgggttc gatccagtcg gcgaacggat cgaaccggtg cccctgtgtc gacaggcgtg
     4441 ccgcgtcgcc ggtcgagaat ggagcgagga gcgccgtgac ggttgccagc acgctgtcgc
     4501 gggttcgcga atcggtctcg acgatcgcgc gcacctgcgc agcctccggt tcgtgaccgt
     4561 gatcggtgag gatttcgatc ggtcgatcga gccgatcggc gacgttccac gacagcacat
     4621 cggcgatgcc gccagagtcg agattcgcgg cgaacaacag cggcccgagc aggctctggg
     4681 cgtaggagat ccagaagtcg gcggccgtca acgagttccg gtcggcgcct tcggcgattt
     4741 gccaggcgat tcgcctcgcc tcggcatagg aggtgcacaa ctgcacgacg tcgaagcgga
     4801 tgcgacgggc cgatgggtgt ggatcggcga ccttgttggc cgggtcgaag agacagatcg
     4861 gacctcgctc ggcccggatg gtcacatagg actgctccca ctcacccgtc ttcgttgtcg
     4921 tcgccacgac ggggccgttg tatccggaca ggaccgtgtt gagcgtgctc gacttgccgc
     4981 atcccgg
//
LOCUS       DAOOMA010000128         4975 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_20370_length_4975_cov_4.438821, whole genome shotgun sequence.
ACCESSION   DAOOMA010000128 DAOOMA010000000
VERSION     DAOOMA010000128.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 4975)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4975)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4975
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20370_length_4975_cov_4.438821"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1131)
                     /locus_tag="PLP95_04810"
     CDS             complement(<1..1131)
                     /locus_tag="PLP95_04810"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013106.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HPB45156.1"
                     /translation="MRRISFAAVLVLVAAVGGAFAFLNSVDVPDVERNIKTTSFVCLG
                     DVADGECTAANSKAQFSEGGNKRIQIGIDEISPNLINAVVAAEDRSFFEHDGVDLFGI
                     GRAAYRGIVGSASRQGASTITQQYVKMVYLSNERTYTRKLREAAIALKIERTMSKREI
                     LEAYLNEAPFGRGAVGIEAAARSYFDKDAGSLSISEAAYLVGLLRAPSYADEPTDPSK
                     PDEATEAKRRRRTVLVGMRELGYITDEEFTAADAEAFEDHVLASAPSFDGTQVRADFE
                     SMGGRYIIDWVRKQLIEDETASMYLGSSALYGGGLRIYLTVDERLQYTAQLATQQVLT
                     AYPGPSAALMSVDDHGQVKAMIGGQDHAASKVNLALGKAGGGS"
     gene            complement(1276..1686)
                     /locus_tag="PLP95_04815"
     CDS             complement(1276..1686)
                     /locus_tag="PLP95_04815"
                     /inference="COORDINATES: protein motif:HMM:NF036456.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5318 family protein"
                     /protein_id="HPB45157.1"
                     /translation="MRGAVSQSRGHGRPGAHPGTVDYRLDRRRLLRAVRAGEVDREEV
                     CDAQLELLRVGRDYSTAAPTPCPLCATRKLRHVRFVFGPRLPKEGRAINSKRELERLS
                     QRTGEYRCYLVEVCMSCRWNHLLSASPLGAQRSA"
     gene            1872..2603
                     /locus_tag="PLP95_04820"
     CDS             1872..2603
                     /locus_tag="PLP95_04820"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HPB45158.1"
                     /translation="MAASVPETSLSTLTYPVGAPFNPTRVRFGPGLADEALDRLIGTP
                     EGKRILELGAGDGSNAIALARRGARVFVVDSSAERLAVARANADAAGVRVEFRHSDLA
                     ELADVRADRLDGCLAVYSLARVVDVARVFRQVHRLLRTEAPLVVSLPHPLELLTHDSS
                     PNEGPPTLTRSAFDDSRIFVGPEQIPVEPHHTADIVTALTRSSFHVDAVLEPPMGSAD
                     GDVFRAPRHDYIPATAIYRGRKLGT"
     gene            complement(2622..3899)
                     /locus_tag="PLP95_04825"
     CDS             complement(2622..3899)
                     /locus_tag="PLP95_04825"
                     /inference="COORDINATES: protein motif:HMM:NF013871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HPB45159.1"
                     /translation="MTSPLDEVTALSTPAATELFEQCRPLGEAFATAGYRLYIVGGSV
                     RDLLLGDTSVEPDLDFTTDALPSESKRLLAPLAQSLWTQGERFGTIAAHVDDRLVEVT
                     THRAESYEPDSRKPNVAYSTEVAVDLGRRDFTINALAIEVTQPEPALIDPFHGLADLC
                     ARRLATPLSPEESFSDDPLRMMRAARFVARFDLVAQRELVDAVESMHHRLSVVSVERI
                     RDEFEKLLSLRDPQRGLAFLIDHSLADGFVPELTESVLGRIDWSLLRRDPHVRLAAIL
                     AVDPRVDATARLRRLRHSKTTVQRVGGVVRGVRTLIDHAGPWSAGDVRRFVAGRFDLV
                     GDIVEVAGALGAAGATELDSAIRELSVRENLAEMSIPIDGRGVMEDLGLAAGRQVGDA
                     LAHLTAHRLEVGPFDRAEATEVLREWLARRSES"
     gene            complement(3896..4900)
                     /locus_tag="PLP95_04830"
     CDS             complement(3896..4900)
                     /locus_tag="PLP95_04830"
                     /inference="COORDINATES: protein motif:HMM:NF013046.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase"
                     /protein_id="HPB45160.1"
                     /translation="MLVLVTDPSSVDHQTGRYHPESPRRYDSVMSAARSPMVRDAVLV
                     AAPRAARPIELAKVHDEAMLERLEHIRGHHVQLDSDTVASPHSVDVAVRSAGTGLTAI
                     DLLDSGAAVAAFCLSRPPGHHATRAQSMGFCLLNNVAVAAVALRDRGDRVAIVDFDVH
                     HGNGTQDIFYDDPNVLYVSWHQSPLYPHTGRLDEIGGPNALGATVNVPLPPMVTGAAY
                     YETLDRVIGPLIERFDPTWLLLSAGFDAHRDDPLSDMGLSSGDFGELTMRLMQVVKPR
                     HTIAFLEGGYDLDALTLSAEATMAALLGLRHHPEAPTSNHEFDPNDLIRMIETTQGYQ
                     "
BASE COUNT          917 a         1719 c         1528 g          811 t
ORIGIN      
        1 cgaaccaccg ccggccttac cgagcgccag attcactttt gaggccgcat gatcctgccc
       61 cccgatcatc gccttcacct gaccatgatc atcgaccgac atcagcgcgg cgctcggtcc
      121 ggggtaggcc gtgagcacct gttgggtggc gagttgcgcc gtgtattgca gccgttcatc
      181 gaccgtcagg tagatgcgaa gaccgccgcc atacagcgct gagctcccga ggtacatcga
      241 cgccgtctca tcctcgatga gttgcttgcg cacccagtcg atgatgtagc gaccgcccat
      301 cgattcgaaa tccgcacgca cctgcgtgcc gtcgaagctc ggcgccgacg ccaacacgtg
      361 atcctcgaac gcttctgcat cggcggcggt gaattcttcg tcggtgatgt agcccaactc
      421 ccgcatgccc acgagcaccg tgcggcggcg gcgcttcgcc tcggtggcct cgtcgggctt
      481 cgacggatcg gtcggctcat cggcataact cggcgcacgc aacaatccca cgagataggc
      541 ggcctccgag atcgacagcg aaccagcgtc cttgtcgaaa tacgagcggg ccgccgcctc
      601 gataccgacc gcaccacgcc cgaacggggc ctcattgaga tacgcctcaa gaatctcccg
      661 cttggacatc gtgcgttcga tcttcaacgc gatcgccgcc tcgcgcagct ttcgcgtgta
      721 ggtccgttcg ttggacaggt acaccatctt cacgtactgc tgggtgatcg tggatgcgcc
      781 ctgccgggac gcagacccga cgattccgcg atacgcggcc cgaccgatgc cgaaaaggtc
      841 gacgccgtca tgttcgaaga aggatcggtc ctccgcagcg accacggcat tgatcagatt
      901 gggtgagatc tcgtcgattc ctatctggat tcgcttgttc ccaccctcag agaactgcgc
      961 ctttgagttc gccgcggtgc attcaccatc tgcgacgtcg ccgagacaca cgaacgaggt
     1021 ggtcttgatg ttgcgttcga catcgggcac gtcgacggag ttgagaaatg cgaaggcccc
     1081 accgaccgcc gcgaccagca ccaacaccgc tgcgaagctg atgcggcgca cccatccggt
     1141 tgcgccgacc ggtcgacgac gcgtcggcga cgcccggccg cggcgtggcg gtgttgacgg
     1201 cgcgggccgc gaacctttgg aggcctggcg cttcgggggc ccggacggac gtcgcgaatc
     1261 ttcggacacg agcgtctagg cgcttcgctg tgccccgagc ggcgacgcgc tcagaaggtg
     1321 gttccagcga cacgacatgc acacctccac gaggtagcag cgatactcgc ccgtacgttg
     1381 cgacagtcgt tcgagttctc gtttggagtt gatcgcccgc ccctccttgg gcagccgcgg
     1441 gccgaacacg aaccgtacgt ggcgcaactt gcgcgtcgcg cacagtgggc acggcgtcgg
     1501 tgcggcggtg gagtagtcgc gaccgactcg caacaactcc agttgggcat cgcacacctc
     1561 ttcgcgatcg acctcgccgg ccctcacggc gcgaagcaac cgtcgacggt cgagtcgata
     1621 gtcgaccgtg cccggatggg cacccgggcg gccatggcca cgcgactggg aaactgcacc
     1681 ccgcaagact accgaccctc actgacacga ccacggcgcc gaacctgagg accgtgtcat
     1741 cgcacgacgc cgccgatcgg cgaggggtcc ccgaccgagc aggttccatc gggcaaagtg
     1801 agaacgtggc tcaacgacct caacgacgac gtcacccctc cgcgggaaac ctgaatgaac
     1861 cgccggaggt cgtggccgcc tccgtgcccg agacatcgct gagcaccctg acctaccccg
     1921 tgggcgcacc gttcaatccg acacgggttc ggttcggtcc cggcctcgcc gacgaggcgc
     1981 tcgaccggct gatcggtacc cccgaaggaa agcgcatcct cgaactcggg gcgggcgacg
     2041 gttccaacgc catcgccctc gctcgccgcg gtgcgcgcgt ttttgtcgtc gactcctcgg
     2101 ccgaacgcct cgccgtcgcg agggccaacg ccgacgccgc aggggtacgg gtcgagtttc
     2161 gccacagcga cctcgccgaa ctcgccgacg ttcgtgccga tcgtctcgat gggtgtctcg
     2221 cggtctattc gctggcgcgc gtcgtcgacg tcgcccgcgt ttttcgccag gtgcaccgac
     2281 tcctgcgcac cgaggcgccc ctcgtggtgt cgctgcccca cccgctcgaa ctcctcaccc
     2341 acgactcgtc gccgaacgaa ggtccgccga ccctgacccg gtcggcgttc gacgactcgc
     2401 ggatattcgt cggacctgaa cagattcccg tcgagcctca ccacaccgcc gacatcgtca
     2461 ccgccctgac ccgctcgagc tttcacgtcg acgccgtgct cgaaccaccg atgggctccg
     2521 ctgacggcga cgtgtttcgc gctccgcgcc acgactacat cccggcgacc gccatctacc
     2581 gaggtcgcaa gctcggaacg taaccggggc accccgggcg gttatgactc cgaccgccgg
     2641 gcgagccatt cacgcaacac ctcggtcgcc tcggcacgat caaacggacc cacctcaaga
     2701 cggtgggccg tcaggtgagc caaggcgtca ccgacctgtc gtcccgccgc caagcccagg
     2761 tcctccatca ccccgcgacc gtcgatcgga atcgacatct ccgcaaggtt ctcccgaacg
     2821 gacagttccc gaatcgccga gtcgagttcc gtcgctccag cggcgccgag cgcgcctgcc
     2881 acctcgacga tgtcgccaac caggtcgaag cgaccggcca cgaaacgtcg cacatcgccc
     2941 gcactccagg gcccagcgtg gtcgatcagg gttcgcaccc cgcgaacaac gccaccgact
     3001 cgctgcaccg ttgtcttcga atggcgcaga cgccgtagcc gggcggtggc gtcgactcgc
     3061 gggtcgaccg ccaggatggc cgcgaggcga acgtgcgggt cacggcgcaa caacgaccag
     3121 tcgatacgcc cgagcaccga ttcggtgagt tcgggcacaa agccatcggc cagcgagtgg
     3181 tcgatcagga acgcaaggcc acgctgcgga tcgcgcaacg acagcagctt ttcgaactcg
     3241 tcgcgaatcc gctcgacgct caccaccgaa agacggtggt gcatcgactc gacggcatcg
     3301 acgagctccc gctgggcgac gagatcgaac cgggcgacga atcgagccgc tcgcatcatc
     3361 cgtaacgggt cgtcagaaaa cgactcctca ggcgacagcg gcgtggcaag acgacgagcg
     3421 cacagatccg cgagcccgtg gaatggatcg atgagcgccg gttctggttg agtcacctcg
     3481 atcgccaacg cgttgatcgt gaagtcgcga cgacccagat cgaccgccac ctcggtgctg
     3541 taggccacat tcggcttacg gctgtcgggc tcataggact cggcgcgatg cgtcgtcacc
     3601 tcgacgagtc gatcatcgac atgcgcagcg atggtgccga aacgctcacc ctgtgtccac
     3661 aacgattgcg ccaacggagc caacaaccgt ttggactccg agggcagcgc gtcggtggtg
     3721 aagtcgaggt cgggctcgac actcgtatca cccagcaaca ggtcgcgaac gctgcctccg
     3781 acgatgtaga gacggtaccc ggcggtcgcg aacgcttcgc caagcggtcg gcactgttcg
     3841 aagagttcgg tggctgccgg ggtcgagagg gcggtgacct cgtcgagcgg cgaggtcatt
     3901 ggtatccctg tgtcgtctcg atcattcgaa tgaggtcgtt cggatcgaac tcgtgattcg
     3961 aggtcggtgc ctcagggtga tggcgaagac ccaacagcgc cgccatcgtc gcctccgcgc
     4021 tcaaggtcaa cgcgtcgagg tcgtagccgc cctcgagaaa cgcgatcgtg tggcgtggct
     4081 tcaccacctg catcaggcgc atcgtcaact caccgaagtc gcctgagctc aagcccatgt
     4141 cgctcaacgg gtcatcgcga tgagcgtcaa agcccgccga caacagcagc cacgtcgggt
     4201 cgaaccgttc gatcagcggt ccgatcacgc gatccagggt ttcgtagtac gcggctcccg
     4261 tcaccatggg aggcaacggc acgttgaccg tggccccgag cgcgttcggc ccgccgatct
     4321 catcgagacg tcccgtgtgt gggtacagcg gagactggtg ccacgacaca tacaagacgt
     4381 tcggatcgtc ataaaagatg tcctgggtgc cattgccgtg atggacatcg aaatcgacga
     4441 tcgccacgcg atcgccacgg tcgcgaagcg cgactgcggc caccgccacg ttgttcagga
     4501 ggcaaaaccc catcgattgt gcccgcgtcg cgtgatggcc gggaggacga gacagacaaa
     4561 aagccgccac cgccgcgccg gaatcgagca gatcgatcgc cgtcaacccg gttccggcac
     4621 tgcgaaccgc tacgtcgacc gagtgaggtg aggcgaccgt gtcggaatcg agttgaacat
     4681 ggtgcccgcg gatgtgttcg aggcgttcga gcattgcctc gtcgtgaacc ttcgccagtt
     4741 cgatcggtcg cgccgcacgc ggcgctgcca ccagaactgc gtcacgcacc atcggcgaac
     4801 gcgccgccga catcaccgag tcgtagcgcc gcggcgactc ggggtgatag cggccggtct
     4861 gatggtcgac cgagctcgga tccgtgacca agaccaacat cggccaccag cgtacgatgg
     4921 ctgggttccc tccgggaact gctggcgcac atcgcctgca ccgacgaccc aaccg
//
LOCUS       DAOOMA010000129         4949 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_20507_length_4949_cov_3.033919, whole genome shotgun sequence.
ACCESSION   DAOOMA010000129 DAOOMA010000000
VERSION     DAOOMA010000129.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 4949)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4949)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4949
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20507_length_4949_cov_3.033919"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..851)
                     /locus_tag="PLP95_04835"
     CDS             complement(<1..851)
                     /locus_tag="PLP95_04835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB45161.1"
                     /translation="MPALPSRPHRSDLVAAVILAATLLWAALAGRRLWFFSDDWNIIA
                     QFPSGDLLEPFNSHLSLVPIGIYQLLFHTVGLESYLPYRIVGLAAYAVLGLMVWRYAS
                     RNLGAVAGAAALTLVLWNAAGVTNVMFPFLMNFSIPVAALAAAWWHLDRTSLRGEVAA
                     SVWLGVALATSGLGLMVAAALGAELLWSRAPVRRWLILAPGPVLWLIWYLAAGTSSPS
                     SGGLRAVAIYAARMLIAGGNGLTGTAGVGGWLVCAALGALLATCLGFGVLNGRIVGAF
                     VAPIVFAV"
     gene            946..2355
                     /locus_tag="PLP95_04840"
     CDS             946..2355
                     /locus_tag="PLP95_04840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB45162.1"
                     /translation="MMLRDRSLDRPRLAVALGAIAVRVGAVVALSRPPEGLHDSLLYL
                     RFAQGISNGQGYVSWYGHKTSYYPPGYPWFLGVLHRVVTAVGLEAHFVMIVGFVQAVL
                     GGIAAWAIVGVAARVVGHDAQRPARSRCARVAGLSPSDSSTALCNTRFGHSAVLLSEG
                     LFVAAFSVSLLGFARLLGDHRADGRSTMTRGWWGDAAIAGVALGVATLTRPQVLTVVV
                     AVGLAAFANQRTARERIALIVAPCLIAAVVVLPWSVRNVIVFDRFVAVSTNGGDNLCI
                     GFNPSATGHFGVPEHCDTGEFYIDGPEAEARRNAETARRARAWAIDHAGELPLLSLKK
                     LRYTYDRDDDALKAIESFGDDEFLSSGARTVLTWISNGTYVVLMVGVVVGAVGLVGAI
                     RRNDPERSLAVLILVGGAVSAVLPIAFFGEARFKVPTTPFFAVMVGYAIARADLHRRG
                     RSDTVDDLPTEPAVQGSEP"
     gene            2352..4070
                     /locus_tag="PLP95_04845"
     CDS             2352..4070
                     /locus_tag="PLP95_04845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB45163.1"
                     /translation="MRRRFDLQTTIALLLLAVAVALPIPGLMGYQGPPMEEGFMLAFP
                     QRILAGAVPHVDFLHLYGPGSLYVLAGVYKVFGDTLEVERIVGIVQHCLLIFAMFWFA
                     RPWGRVSALFAGLITLVITISPLGLSAMAWNGALGLAMAGIAVLVPGLDGGGRVRLST
                     AGVCFGAALLYRPDMIVAIGLTCVIAWLMLGRDPAARRDRWYGLAGAVVTSALIVPFL
                     IAVGFERAIEGMFLQPVFDLRDGRTLPVPPSWSEPDGYLQKAGGLRVSRWPLPMLEIG
                     PQIALWFWLVPVSVLFVVFAAWRCCKVQPERLRPRGLAVGAAFALGLLPQAFQRPDTA
                     HLSWVSCVSFALVPLAILELWTIRTPLAGEVPSGVTVKQGIVGLVPALRRSLARPVAA
                     VLPILFVLVAVIPFYPLRTYADLVGQSFGHNVFGYEIVRGNRRFFYGSEEAAANAQRI
                     VDRLDEESNAGQRLIVAPYDLALTPYSDAFFYYLFPELVPGTRYIEMDPGLADAVGSG
                     LAEELANNDWLILSDVWSGWEEPNSSVERGDERPNEIVARDYCEVLDAAPFRLLQRCD
                     RTRRGS"
     gene            4202..>4949
                     /locus_tag="PLP95_04850"
     CDS             4202..>4949
                     /locus_tag="PLP95_04850"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenol monophosphomannose synthase"
                     /protein_id="HPB45164.1"
                     /translation="MRTALVVPTYQEAGNIERFLREVRAALPEADVFVFDDNSPDGTG
                     ELAERVAEELGRIDVVHRPSKEGLGAAYRHGISHVLGLGYEIVMQMDVDFSHDPAVLP
                     RLRQAILDGADVAVGSRYVPGGATPDWPLRRRMLSKYGNTYSRVMLGLVFNDATAGFR
                     AYRAPVLEQISFHTTRANGYGFMIETGFRLSDAGATVTEVPIIFRDRTVGESKMAIPT
                     MVETMSMVTWWGLCRRAPKLTGRFRTTRLGE"
BASE COUNT          764 a         1627 c         1677 g          881 t
ORIGIN      
        1 acggcgaaca cgatcggcgc gacgaacgcc ccgacgatgc gcccgttcag caccccgaaa
       61 ccgagacacg tcgccaacaa cgcacccaac gctgcacaca ccaaccagcc gcccaccccc
      121 gccgtgccgg tgagtccgtt tccgccggcg atcagcattc gcgcggcata gatcgcgacg
      181 gcgcgcaacc cgcccgaact cggcgatgag gtgccagcag cgagatacca gatcagccag
      241 agcaccggcc cgggggcgag gatcagccag cggcgaacgg gtgcacgcga ccacaacagt
      301 tcggcaccca gggcggccgc caccatcaac ccgagcccgc tcgtcgccaa ggcgacaccg
      361 agccagaccg aggcggcgac ctcgccccgg agcgaggtgc ggtcgagatg ccaccaggcc
      421 gctgcgagcg ccgcgacggg aatcgagaag ttcatgagga acgggaacat cacgttggtc
      481 accccggccg cgttccacag caccaacgtc agcgctgcgg ctccggcgac cgcaccgagg
      541 ttcctactcg catagcgcca gaccatgagt cccagcaccg cgtaggcggc gaggccgacg
      601 atgcggtacg gcaggtacga ctcgaggccg accgtgtgga acagaagttg gtagatcccg
      661 atcggaacca gcgacaggtg actgttgaac ggttcgagaa ggtctccgct cggaaactgg
      721 gcgatgatgt tccagtcgtc gctgaagaac cagagccgac gaccggccag ggcggcccac
      781 agcagtgttg cggcgaggat caccgccgcg acgaggtcgc tgcggtgagg ccgtgagggc
      841 agtgctggca cgaccgcgat tctcgcagaa ccagtcgacc acgtcgcgca tacgcggcgg
      901 cacccggtcg acgaagtcct atgcggtgcc aactaacctc gccgcgtgat gttgcgtgat
      961 cggagcctcg accgtccccg gctcgcggtc gccctcggcg cgatcgcggt gcgagtcggc
     1021 gcggtcgtgg cgctgtcgcg tccccctgag ggccttcacg attcgctgct gtacctgcgg
     1081 ttcgctcaag gtatttccaa cggccagggt tacgtcagtt ggtacggcca caagacctcg
     1141 tactacccgc ccgggtaccc gtggtttctc ggcgtcctgc atcgtgtcgt gaccgcggtg
     1201 ggcctcgagg cccactttgt gatgatcgtg ggctttgtcc aggcggtgct cggcggcatc
     1261 gccgcgtggg cgatcgtcgg ggtcgcagct cgcgtcgtcg gccacgatgc gcaacggccc
     1321 gctcggagcc gatgcgcccg cgtggcgggg ttgagcccct cggacagcag cacggcgctg
     1381 tgcaacacca ggttcggcca cagcgccgtg ctgctgtccg aggggctctt cgtggccgcc
     1441 ttttcggtgt cgctgctcgg cttcgcccgc cttctcggcg accaccgggc agatgggcgt
     1501 tcaacgatga cccgcggttg gtggggcgac gccgcgattg ccggggtcgc actcggggtg
     1561 gccacgctga cccgaccgca ggtgctcacc gtggtcgtcg ccgtgggcct cgcagcgttc
     1621 gccaaccagc gaacggcgcg ggaacggatc gctctcatcg tcgctccctg cctgatcgcc
     1681 gcggtcgtcg tgctgccgtg gagcgttcgc aacgtcatcg tgttcgaccg cttcgtcgcc
     1741 gtgtcgacca atggcggtga caacctctgc atcgggttca acccgagtgc gaccgggcat
     1801 ttcggcgtac ccgagcactg cgacaccggc gagttttaca tcgacggccc ggaggccgag
     1861 gcgcggcgta acgccgagac cgcccggcgc gcccgagcct gggcgatcga ccatgccggc
     1921 gagttgccgc tgctgagcct caagaagctg cgctacacct acgaccggga cgacgacgcg
     1981 ctcaaggcca tcgaatcctt tggcgacgac gagtttctgt cgtccggggc gcgcaccgta
     2041 ctcacgtgga tcagcaacgg cacctacgtg gttctgatgg tcggggtggt cgtcggtgcc
     2101 gtcgggttgg ttggggcgat ccggcgcaac gaccctgagc ggtcgctggc cgtgttgatc
     2161 ctcgtggggg gtgccgtgtc ggcggtgttg ccgatcgcct tcttcggcga ggcccgtttc
     2221 aaggtcccca cgaccccgtt tttcgcggtg atggtcggct acgcaatcgc ccgcgcggac
     2281 cttcaccgtc gtggccggtc cgatacggtc gacgacctgc caaccgagcc agccgtgcaa
     2341 ggaagcgaac catgaggcgc cgattcgatc tgcagaccac gatcgccctg ctgttgctgg
     2401 ccgtcgccgt ggcgctgccc atacccggtc tcatgggcta tcagggaccg ccgatggagg
     2461 aggggttcat gttggccttc ccccaacgga tcctcgccgg cgcagtaccc cacgtcgact
     2521 tcctgcacct ctacgggccg ggatcgctgt acgtgctcgc cggtgtctac aaggtgttcg
     2581 gagacaccct cgaggtcgaa cgcatcgtcg gcatcgtcca gcactgcctg ttgatcttcg
     2641 cgatgttctg gttcgccagg ccctgggggc gggtctccgc gttgttcgcc ggcctcatca
     2701 ccctggtgat caccatctct ccgctcggat tgagcgccat ggcgtggaac ggggcgctcg
     2761 gtctggcgat ggcgggaatc gcggtgctgg tccccggcct cgacggtggc gggcgggtgc
     2821 ggctttcgac ggcgggcgtg tgcttcggcg ctgcgctgct gtaccggccc gacatgatcg
     2881 tggcgatcgg gctgacctgt gtgatcgcct ggctgatgct cggccgagac cccgccgccc
     2941 gccgcgatcg gtggtacggc ctggcgggag cggtggtgac atcggcattg atcgtgccgt
     3001 tcctgatcgc cgtcggattc gaacgggcca tcgagggaat gttcctgcaa cccgtgttcg
     3061 acctgcgcga cggacgcacg ctgccggttc ccccaagctg gtcggaaccc gacggctacc
     3121 tgcaaaaggc cgggggattg cgtgtgtcga ggtggccgct gccgatgttg gagatcggcc
     3181 cacagatcgc gctgtggttc tggctcgtgc cggtttcggt gctgttcgtg gtgttcgccg
     3241 catggcgttg ttgcaaggtg caacccgaac ggctgcggcc tcgggggctc gcggtgggcg
     3301 ccgcgtttgc actcggcctg ttgccccagg cgttccaacg gccagacacc gcccacctgt
     3361 cgtgggtgtc gtgtgtgagc ttcgcgctcg tgccgctcgc gatcctggaa ctgtggacca
     3421 tacgtacccc gctcgctggt gaggtcccct ccggggtgac ggtgaaacag ggcatcgtcg
     3481 gcctcgtccc ggcgcttcga cggtcgctcg ccaggccggt ggccgccgtg ctgccgattc
     3541 tgttcgtgct tgtggccgtc attccgttct acccgctgcg aacctacgcg gacctcgtcg
     3601 ggcagagctt cggccacaac gtgttcggct acgagatcgt gcgcggaaac cgacgcttct
     3661 tctacggcag cgaggaggcg gcagcgaacg cacaacgcat cgtcgaccgg ctcgacgagg
     3721 agtcaaatgc agggcaacgg ctgatcgtcg ctccctatga tctggcgctc acgccgtaca
     3781 gcgacgcgtt cttctactac ctgttccccg aactcgttcc tggcacgaga tacatcgaaa
     3841 tggacccggg gctcgcggat gcggtgggtt caggacttgc cgaggagttg gcgaacaacg
     3901 actggctgat cctgtccgac gtctggagcg gctgggagga acccaactcg agcgtcgagc
     3961 gtggcgacga acgaccgaac gaaatcgtcg cccgggacta ttgcgaggtg ctcgacgcag
     4021 cgccgttccg gttgcttcaa cgttgtgacc ggacccggcg aggatcgtga ccgaccccac
     4081 gtgacgccgc ctgtgcccgc caacgggtgg gtgcatgtat ccggtgggtg catgtatccg
     4141 gtgggtgcat gtatccggtg ggtgcatgta tcgggtgggg cgcatcagta gcattgcccg
     4201 aatgcgtacc gctctggtcg ttcccaccta ccaagaggcc ggcaacatcg aacgtttctt
     4261 gcgcgaggtc cgcgcggcgt tgccagaagc cgacgtgttc gtgttcgacg acaacagccc
     4321 cgacggtacc ggcgagttgg ccgagcgggt cgctgaggaa ctgggccgga tcgacgtggt
     4381 gcaccgaccg tccaaggagg gcctcggcgc cgcgtaccgt cacggaatct cccacgttct
     4441 cggcctcggc tacgagatcg tgatgcagat ggacgtcgat ttctcccacg acccggcggt
     4501 gcttccccgg cttcgtcagg cgatcctcga cggcgccgat gtcgcggtcg ggtcgcggta
     4561 tgtgcccgga ggcgccaccc cagactggcc gcttcgacgt cggatgttgt cgaagtacgg
     4621 caacacctat tcccgggtca tgctggggct ggtgttcaac gatgccaccg cgggttttcg
     4681 cgcctaccgc gcaccggttc tggaacagat cagctttcac accactcgcg ccaatgggta
     4741 tggattcatg atcgagaccg gatttcggct cagcgacgcg ggggcgacgg tcaccgaagt
     4801 gccgatcatc tttcgggatc gcacggtcgg cgagtcgaag atggcgatcc cgacgatggt
     4861 cgagaccatg tcgatggtga cgtggtgggg tctgtgtcgg cgcgcgccga aactcacggg
     4921 acgattccgc accacccgcc tgggcgagc
//
LOCUS       DAOOMA010000130         4815 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_21231_length_4815_cov_4.176471, whole genome shotgun sequence.
ACCESSION   DAOOMA010000130 DAOOMA010000000
VERSION     DAOOMA010000130.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 4815)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4815)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4815
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21231_length_4815_cov_4.176471"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..212
                     /locus_tag="PLP95_04855"
     CDS             <1..212
                     /locus_tag="PLP95_04855"
                     /inference="COORDINATES: protein motif:HMM:NF014608.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HPB45165.1"
                     /translation="RRFRPWWGLLREKRVDRGIQAITRIELRPTVEWSGDAPTADVVD
                     RLHHEAHRRCNIANSIKAEVTISSR"
     gene            complement(239..715)
                     /gene="smpB"
                     /locus_tag="PLP95_04860"
     CDS             complement(239..715)
                     /gene="smpB"
                     /locus_tag="PLP95_04860"
                     /inference="COORDINATES: protein motif:HMM:NF003843.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HPB45166.1"
                     /translation="METPEIKVVATNRAARHDYHLLDEFEAGIVLRGSEVKAMREAQV
                     QIKEAFCQISNGELWIHNLHIAPYAFSQTHSGHDPLRVRKLLLHRREIDKIRRKQMAD
                     RLSIVPTRIYFKDGRIKVEIALAKGKTKGDRRQDIAKKDAEREARNEMGRRLKYGA"
     gene            774..1193
                     /locus_tag="PLP95_04865"
     CDS             774..1193
                     /locus_tag="PLP95_04865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015289775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M67 family metallopeptidase"
                     /protein_id="HPB45167.1"
                     /translation="MLRLTVDIHRAMLAHAFDGLPDEACGLMVGSLGGDDGVASLVRF
                     EPCRNAAQSSRIYSIDPKEWMRIERDADDDELDIVGVMHSHTHTEPYPSVTDVDQAPD
                     PAWHYVIVSLRDSSPILRSYRIVDGEIIEEPVEIVPA"
     gene            1289..2236
                     /locus_tag="PLP95_04870"
     CDS             1289..2236
                     /locus_tag="PLP95_04870"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase family protein"
                     /protein_id="HPB45168.1"
                     /translation="MTIFSSVFDMIGNTPMVDVSRLSPNPDVRILAKMEGQNPGGSVK
                     DRIARQMILEAEKDGRLTPGATLLEPSSGNTGIALAMICQERGYHLKILMPESVSIER
                     RQMLEVFGAEITLTPGGEGSNGAVRRAVALAEEHPEWVFLYQYANEANPRAHYLTTGP
                     EIWADCPEITHFVAGLGTSGTLLGVGTYLKERNANVQILAVEPPAGELVEGLRSLDDG
                     YIPPVFEKWKGLELLNGKSIVRPLESLEYARKLTTVGVFPGISAGAAFAGACKVANRI
                     DSGTIVFIVSDYGWKYLSTGAFTDDLEQAAANAESIIYF"
     gene            2402..>4815
                     /locus_tag="PLP95_04875"
     CDS             2402..>4815
                     /locus_tag="PLP95_04875"
                     /inference="COORDINATES: protein motif:HMM:NF024642.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HPB45169.1"
                     /translation="MIEQLTRTRAPSNGETDEPAVESTTAASTAAATEALSLFQATND
                     EEFSRVRLDNEQVHADEVAATEAAGRAGERPTARPVLFGRLDLVDPEGLTETVYLGPA
                     GRWDAKSGRQLVVDWRSDLGATFNRATRHDPMGVGRRRVVAMRDDEVVGVHDETLTDG
                     FHAGDPGDAMQLVLDLTDGSNPPSLTQPVTSAVTAAVTPVGTFDLGLSTDGDVSGPAA
                     EWANSAGRPSVWASEVELDRIGVSLRHPVGLVSALSRPTAQRRGHVLETMQADQDWLV
                     RCDPTTAIVIEGPAGTGKTTVGLHRVAWVLHELGVGAPGTAASALVLTPNESARQHAL
                     EVLEGLGVSSASVDTIETLLGHKAGDPAAGAQTLSRPRADDEAEALKGDVRLAGLLRV
                     AVWMGCRANAVTVVERGVRAVLGRARVEAIIESVFDTGVSYDDGRHGVMATLATELAD
                     QIIARKVDGVYGGAFGLAPQADQNAASASERRVAMVQRIARELRSSGQLDALLPDQQA
                     AEVLERLLGDDGFRQRANDDLSAAEVALLGRSKGAIRAWTAADLVLIDELSFILHGRS
                     DRFDHVFVDDATDLSPMQWRCVERRLLGESITIAGDLGQDVSPWSDGTTDRSWSRIGE
                     LLELENGLDVKRCVLAYRRPLDLDDPVAVLEPIPIEQRSVEPGEVEQWILQTVIERAE
                     TGGSVVVITEESNVIRIDRLLRRPVLNDADISVVADRDISGHEADHVVLVKPTEVVRS
                     DGERGRRRLRRALSRATHSVTVIAERDSVSARDLVSRSVPRPAATGASELLEFALLVR
                     LRAALSV"
BASE COUNT          797 a         1569 c         1609 g          840 t
ORIGIN      
        1 tccgtcggtt tcggccatgg tggggcttac tgcgcgagaa acgcgtcgat cgcgggatcc
       61 aggcgatcac caggatcgaa ctgcgcccga ccgtcgaatg gtccggcgac gccccgacgg
      121 ccgacgtcgt cgaccgcctg caccatgagg cccatcggcg ctgcaacatc gccaactcca
      181 tcaaggccga ggtcaccatc tcctcgcgct gagccggcgg cgaccctccg acgaccgatc
      241 aggcgccgta cttgaggcgt cggcccatct cgttgcgggc ctctcgctcg gcgtccttct
      301 tcgcgatgtc ctggcggcgg tcgcccttgg tcttaccctt ggccagggcg atctccacct
      361 tgattcgccc atctttgaag tagatccgcg tcggcacgat cgagagccga tcggccatct
      421 gcttgcggcg gatcttgtcg atctcgcgcc gatgcaacag cagctttcgg acccgaagcg
      481 gatcgtggcc tgaatgggtc tggctgaacg cgtacggcgc gatgtgcaga ttgtgaatcc
      541 acagttcgcc gttggagatc tgacagaacg cctctttgat ctgcacctgc gcctcgcgca
      601 tcgccttcac ttcgcttccg cgcaaaacga tgccggcctc gaactcgtcg aggaggtgat
      661 agtcgtggcg ggcggcccga ttggtcgcga ccaccttgat ctcgggcgtt tccataaggg
      721 agccatgcta cccgtggccc gagcgcgccg atcgagcggc ctatcgtggt gacgtgttgc
      781 gcctcaccgt cgatatccac cgtgcgatgc tcgcccacgc cttcgacgga ctgcccgacg
      841 aggcctgcgg gctgatggtc gggtcgctcg gaggcgacga cggcgtcgcg tcactcgttc
      901 gcttcgagcc atgtcgcaac gcggcgcagt cgtcgcggat ctactcgatc gatcccaagg
      961 agtggatgcg catcgagcgc gacgccgacg acgacgaact cgacatcgtc ggggtgatgc
     1021 acagccacac ccacaccgag ccgtatccgt cggtcaccga cgtcgaccag gctcccgacc
     1081 ccgcgtggca ctacgtgatc gtgtcgctgc gcgattcgtc gccgatcctg cgttcctatc
     1141 gcatcgtcga cggtgaaatc atcgaagaac cggtggagat cgttcccgcc tgacccggcc
     1201 gacgccagcg gggaacctgc tcggatttct taccaaaagg gtcgggattt agactgggcg
     1261 agtcccggca gtcgcgaggt atgcccgaat gaccatcttc tcttccgtgt tcgacatgat
     1321 cggcaatacg ccgatggtgg acgtgtcgcg tctcagcccg aatcctgacg tgcggatcct
     1381 ggcgaagatg gaggggcaga accctggcgg ctcggtgaag gaccgcatcg cccgccagat
     1441 gatcctcgag gccgaaaagg acggacgcct cacccccggg gcaacgctgc tcgaaccgtc
     1501 ctcgggcaac accggcatcg cgctcgccat gatctgccag gagcgcggct accacctcaa
     1561 gatcctcatg cccgagtcgg tgtcgatcga acgccgccag atgctcgagg tgttcggagc
     1621 cgaaatcacc ctcaccccgg gcggcgaagg ttcgaacggc gcggtgcgtc gggccgtcgc
     1681 gctcgccgag gaacatcccg agtgggtgtt cctctaccag tacgcgaacg aggccaaccc
     1741 gcgggcccac tatctgacga ccgggccgga gatctgggcg gactgccccg agatcaccca
     1801 tttcgtggcg ggccttggca cctcggggac gctgctcggc gtcggcacct acctcaagga
     1861 acgaaacgcg aacgtgcaga tcctcgccgt cgagcctccc gccggtgagc tcgtcgaagg
     1921 gcttcgctcc cttgatgacg gctacatccc gccggtgttc gagaaatgga agggtctcga
     1981 gttgctgaac ggcaagtcga tcgtgcgccc actcgaatcg cttgaatacg cccgcaagct
     2041 caccaccgtg ggggtgtttc ccggcatctc ggcgggtgcg gcgttcgccg gagcctgcaa
     2101 ggtcgccaac cgcatcgact cgggcacgat cgtgttcatc gtcagcgact acggctggaa
     2161 gtacctctcc accggagcct tcaccgacga cctcgaacag gccgcggcca acgccgagtc
     2221 gatcatctac ttctgagccc gcacttcctc ggcggagaac caccaatttg cggtgtggcc
     2281 gccctaatgt cgaccgtact gtcggctggg agggttgctc gtgcgagcgc gaggttctcg
     2341 aggacgagtt ggtcgtggag gtgtgttcgt cggcgcgggc gtgcgcagcc gaatcgaccc
     2401 cgtgatcgaa cagttgaccc gcacccgagc cccgtcgaac ggtgaaaccg acgagccggc
     2461 ggtggaatcg acgacagcgg catcgacggc cgcggcaacc gaggccttgt cgctgtttca
     2521 ggcaaccaat gacgaagagt tcagccgcgt tcgtctcgac aacgaacagg tccatgcgga
     2581 cgaagtggcg gcgaccgagg ctgcgggtcg agccggcgag cgcccgaccg ctcgtccggt
     2641 gttgttcggg cgcctcgatc tggtcgaccc cgaaggtctc accgagacgg tgtatctggg
     2701 cccggcgggg cgatgggacg cgaagtcggg acgtcagctc gtcgtcgact ggcgcagcga
     2761 tctgggtgcg accttcaacc gcgcaacacg gcacgacccc atgggcgtcg gccggcgcag
     2821 ggttgtggcg atgcgcgacg acgaggttgt cggcgttcac gacgagacgt tgaccgacgg
     2881 gttccacgca ggcgacccgg gcgacgccat gcaactcgta ctcgacctga ccgacggctc
     2941 gaaccctccg tcgctcacgc agccggtcac ttcagcggtt accgcagcgg tcacgccggt
     3001 gggaaccttc gatctgggtc tctccaccga cggcgatgtc agcggtcccg cagcggagtg
     3061 ggccaactcg gccggtcggc catcggtgtg ggcgagcgag gtcgaactcg accgaatcgg
     3121 tgtttcgctt cgccacccgg tcgggctcgt ttcggcgctg tcccggccga ccgcacaacg
     3181 ccgtggccat gtccttgaga ccatgcaggc cgaccaggac tggctcgtgc gttgcgaccc
     3241 gaccacggcg atcgtgatcg agggcccggc cggcaccggc aagaccacgg tcgggttgca
     3301 ccgtgtggcc tgggttctgc atgaactggg ggtcggcgca ccaggtacgg ccgcgagcgc
     3361 gctcgtgttg acgcccaacg agtcggcgcg ccaacacgcg ctcgaagtgc tcgaagggct
     3421 cggcgtgtcc agtgcttcgg tcgacacgat cgaaacgctc ctcgggcaca aggcaggcga
     3481 cccggcggcg ggtgctcaaa cgctttcgcg gccccgagcc gacgacgaag cggaagccct
     3541 caaaggcgac gtgcgactcg ctgggctact gcgggtcgcg gtctggatgg gctgtcgcgc
     3601 caacgcggtc acggtcgtgg aacgcggcgt gcgcgccgtg ctcgggcggg cgagggttga
     3661 ggcgatcatc gagtcggtgt tcgacaccgg ggtgtcctac gacgacggcc gccacggcgt
     3721 gatggcgacc cttgccaccg agctcgccga tcagatcatc gcccgtaagg tcgatggcgt
     3781 ttacggcggt gccttcggtc tcgcccccca agccgaccag aacgccgcga gcgcgtcgga
     3841 acgacgcgtg gcgatggtgc agcgaatcgc tcgagagttg cgctcgtctg ggcaactcga
     3901 tgcgctgttg ccggatcagc aggcggccga ggtgttggaa cggctgctgg gcgacgacgg
     3961 tttccggcag cgggccaacg acgatctgag cgccgctgag gtggcgctcc tgggtcgttc
     4021 gaagggtgcg atacgcgcct ggaccgccgc ggatctggtc ttgatcgacg agctttcctt
     4081 catccttcac ggccgatccg atcgcttcga tcacgtcttc gtcgacgatg cgaccgacct
     4141 gtcgccgatg caatggcggt gcgtggagcg gcgcctgctc ggcgaatcga tcacgatcgc
     4201 cggcgatctc ggccaggacg tttcgccgtg gagcgacggc accacagacc ggtcgtggtc
     4261 gcgcatcggc gaactgcttg agttggaaaa cggcctggac gtgaagcgtt gtgtgctcgc
     4321 ctaccgtcgc ccgctcgatc tcgacgatcc cgtcgcggtg ctcgaaccga taccgatcga
     4381 gcagcgcagt gtcgagccgg gcgaagtgga gcagtggatt ctgcagacag tcattgaacg
     4441 ggcggaaacc ggggggagtg tggtcgtgat caccgaagaa tcaaatgtca tacgtatcga
     4501 ccgtctgttg cgacgaccgg tgctcaacga cgctgacatc agcgtggttg ccgatcggga
     4561 catcagcgga cacgaggccg accacgtggt gctcgtgaaa cccaccgagg tcgttcgctc
     4621 tgacggagag cgcggtcgcc gccgacttcg cagggcgttg agccgagcga cgcattcggt
     4681 gacggtgatc gcggagcgtg actcggtttc ggctcgcgat ctcgtgtctc gaagcgtgcc
     4741 gcgcccggcc gctaccgggg cgtcggagtt gctcgagttc gcgttgctgg tccgcctgcg
     4801 cgcagccctg tcggt
//
LOCUS       DAOOMA010000131         4809 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_21267_length_4809_cov_2.826252, whole genome shotgun sequence.
ACCESSION   DAOOMA010000131 DAOOMA010000000
VERSION     DAOOMA010000131.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 4809)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4809)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4809
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21267_length_4809_cov_2.826252"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1375
                     /locus_tag="PLP95_04880"
     CDS             <1..1375
                     /locus_tag="PLP95_04880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB45170.1"
                     /translation="LERRLFDEIGGFDERLVLTGNDVELSVDALRRGLRNVLTSGARL
                     RHFESVTRGNSTVWDDTFRSYWRFRHWIAAGDPRFSQNLRIIGPTPSWTRPWDPAPLQ
                     LMEQHFHRRLDPRLDDDLPSTALQAAWNELPAATHRRVDGPLSTVVWVASHLTGPTDP
                     RVEIALEVAARLAHMGIESVFAVAAVEDPLWFEAIVSGASRGCPSHRVVHLGSHAERP
                     LPDGDALVAIDPAIRPLIAADPRRGLAIAVDPDEVIRDLDLLCNNAAAASAHAALGGR
                     SLVIEPHIAAVPRPAPAPGAPAAQPTTRSIIFDVDGAADRVAWEATIETVGSLKAQLG
                     TGIRLVVSADELDEAVKLRLGDAEIVIRPDERSLWRYCQSGDLVVCLSSPLTQPRRAR
                     WWRAATPDVAVASAPGSPEGGHRSHHKPLSAPLVQAWLNGEAPIEPLEHAEPVDPQRL
                     IDFLRGT"
     gene            complement(1438..2655)
                     /locus_tag="PLP95_04885"
     CDS             complement(1438..2655)
                     /locus_tag="PLP95_04885"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPB45171.1"
                     /translation="MADAAISVEGVSKQFRLYTESTRSIKEQFTRRKTGRYNEFWALQ
                     DVDLTVERGEVFGFVGHNGSGKSTLLRCIAGIYRPTKGRVATNGRISALLELGAGFHP
                     ELTGRENIFMNATILGFSRSEIEPLVPKIAEFTGLGEFLDVPVKVYSSGMFVRLGFAV
                     AVNVQPEILIIDEVIAVGDEEFQRKCFEHLYQLRKQGVTIVMVSHGLGLIQTMCDRAM
                     WLERGKVQSIGPALEVVEDYIHKVNRDETQSDVAAPTGGMAEYGRQGSGEIAVTSVQF
                     LDRTGDQVGFLPSRDPLTIRMFYRASEPVESPAFAISVHSDAGFHLTGQWCGEDFGRV
                     SGEGYVDFHIDDMRLMPGDWEVSTSIYDRNRGHVFDRLERAFHLTIRPNGDSDAFGHV
                     KTIGEWSIHRGAS"
     gene            complement(2655..3476)
                     /locus_tag="PLP95_04890"
     CDS             complement(2655..3476)
                     /locus_tag="PLP95_04890"
                     /inference="COORDINATES: protein motif:HMM:NF013245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPB45172.1"
                     /translation="MVVSNSPVRIWQYRNLIYHLAQRDLKARHKKSILGWLWSLINPA
                     ATLGIYTLVFGVFLKVEPPVAGDGSTKVFALYLFCALIAWNFFNSVINGSMMAFQASG
                     GMLTKVYFPPECPAIANMCVALVQFGIELCIALAFLIGLGVVSWTAVFALVTIVQLAM
                     FSIGIGLVVSLWNVRYRDVSYLIGIGLQLLFYATPIVYRAEIIPEKLGGWFPIRRVFE
                     LNPMTQFVTNMRKALYTLEAPSLGNLAVMSVVSLVVACGGWLLFARSAPGVIEEL"
     gene            complement(3476..>4809)
                     /locus_tag="PLP95_04895"
     CDS             complement(3476..>4809)
                     /locus_tag="PLP95_04895"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HPB45173.1"
                     /translation="KVSAVIDAHYEDIDYIYTDEDKLTEDGFRHNPFFKPDWSPERFR
                     YQMYTCHFATMRASLVREVGGFRAEYNGSQDFDLVFRVTERARRIVHIPEILYGWRII
                     SGSAAGDVNAKPYAWIAGQKAIQSHCDRTGFEAHVTHDLAQPGWYRLEPRLTQSPLVS
                     IVIPTAGSAKLIRGAVEPVATACLRTIDEVSTYENYEVVVVADIHTDPRVRAVAEERF
                     GGRLTWVEFDKPFNFSEQINLGVLNSRGDHLLLLNDDIEITTPNWIESMLMYSAQEAI
                     GAVGVKLVYGDGRLQHAGVTTANAESGPAHVCMGFPGDAPGSFSVLRLGNNYLAVTAA
                     AMMTRRDAFFEVGGFSEQFPSSYNDVDYCLKLRETGYRVAFNPEVELVHHESASRDHN
                     VSPVETRDFNERWSHYIGNDPFYNPNLVFNPSDFGIPGSYVLNERRPAVRS"
BASE COUNT          917 a         1603 c         1447 g          842 t
ORIGIN      
        1 gctcgaacgt cggcttttcg acgagatcgg aggcttcgac gagcgactcg tgctgaccgg
       61 aaacgacgtc gagctctccg tggacgcgct gcgccgcggt ctgcgcaacg tcctcacctc
      121 gggggcccgg ctgcgccact tcgagtcggt gacccgaggc aacagcaccg tgtgggacga
      181 cacgtttcgc agctactggc gctttcgaca ctggatcgca gcaggtgacc cacgcttcag
      241 ccaaaacctg cgcatcatcg gcccgacccc gtcatggacg cgtccgtggg atccggcacc
      301 tctgcagctg atggagcagc acttccatcg tcgcctcgat ccgcgactcg acgacgacct
      361 gccctcaacc gccctccagg cagcgtggaa cgagctccct gccgctaccc accgacgtgt
      421 cgatggtccg ctgtccacgg tcgtctgggt cgccagccat ctcaccggcc ccaccgaccc
      481 ccgcgtcgag atcgcgctcg aggtggccgc gcgcctcgcc cacatgggca ttgagtcggt
      541 gttcgccgtc gctgcggtcg aggacccgct gtggttcgaa gccatcgtgt caggggcatc
      601 tcgcggctgt ccgtcgcatc gcgtcgtcca cctcggctcc cacgccgaac ggccgcttcc
      661 cgacggcgat gccctcgtgg cgatcgaccc ggccatccgt ccactcatcg cggccgatcc
      721 gcgtcgaggc ctcgccatcg ccgtcgaccc cgacgaggtg attcgtgacc tcgatctcct
      781 ctgcaacaac gccgcggcag catcggctca cgccgcactc ggcggtaggt cgttagtcat
      841 cgaaccgcac atcgccgctg ttccccgccc tgctcccgcc cctggtgctc ctgccgctca
      901 gccgacgaca cgctcgatca tcttcgatgt cgatggcgcc gccgaccgcg tggcctggga
      961 agcaacgatc gagacagtgg gctcactcaa ggcccagctt ggcaccggta tccgactcgt
     1021 ggtgagcgcc gacgaactcg acgaagccgt caaactcagg ctcggtgacg ccgagatcgt
     1081 cattcgcccc gatgagcggt cattgtggcg atactgccag tctggagatc ttgtcgtgtg
     1141 cctgtcctcc ccgctgaccc agccgcggcg ggctcgttgg tggcgtgccg ccacaccaga
     1201 cgtcgccgtc gcgagcgccc ccggttcccc cgagggcggt caccgcagcc atcacaagcc
     1261 tctcagcgcc ccactcgtac aggcgtggct gaacggagag gccccaatcg aacctcttga
     1321 acacgccgag cccgtcgacc cacagcggct catcgacttt ctccgaggaa cctgacaccc
     1381 tgcggccacc tcgagcgcta gcctcagcgc cggcatcccg gctcagggtg tctcggctca
     1441 ggacgcgcca cggtggatcg accactcacc gatggtcttc acatgcccga acgcatcgct
     1501 gtccccgttg ggacggatgg tgaggtgaaa cgcccgttcg agccgatcga acacgtggcc
     1561 gcggttgcgg tcgtagatcg acgtcgacac ctcccaatca ccgggcatca agcgcatgtc
     1621 gtcgatgtga aagtcgacat acccctcacc gctcacgcgt ccaaaatctt ccccacacca
     1681 ttgaccggtc aggtggaaac ctgcatcgga gtggaccgag atcgcgaagg cgggggattc
     1741 gacgggttcg gacgcccggt agaacatccg gatcgtcaac ggatcacgcg agggcaaaaa
     1801 gccgacctgg tcgccggtcc ggtcgaggaa ctgaaccgag gtcaccgcga tctcgccgga
     1861 tccctggcgc ccgtactccg ccattccacc cgtaggcgcc gcaacgtcgg actgggtctc
     1921 gtcgcggttg accttgtgga tgtagtcctc gacgacctcg agagccggcc cgatggactg
     1981 caccttgccc cgttcgagcc acatcgcacg atcacacatc gtctggatca gtcccaaccc
     2041 gtgagacacc atcacgatgg tgactccctg tttgcggagc tgatagaggt gctcgaagca
     2101 tttgcgttgg aactcctcgt caccgacggc gatcacctcg tcgatgatca ggatctccgg
     2161 ctggacattg accgcgacgg cgaaaccgag ccgcacgaac atgcccgagg agtacacctt
     2221 gaccggtacg tcaagaaact caccgagccc ggtgaattcg gcgatcttcg gcacgagcgg
     2281 ctcgatctcg gaacgcgaga agccgaggat ggtcgcgttc atgaaaatgt tctcgcgccc
     2341 agtcagctcc gggtgaaacc cggcaccgag ttcgaggagg gccgagatgc gcccgttggt
     2401 ggcgacccgg cccttcgtcg gccggtagat cccggcgatg cagcgcaaca gggtcgattt
     2461 acccgaaccg ttgtggccga cgaaaccgaa aacctcgccc cgttcaaccg tcaggtcgac
     2521 gtcctgtagc gcccaaaact cgttgtagcg gccggtcttg cggcgcgtga actgttcttt
     2581 gatcgaccgg gtgctctcgg tgtagagccg gaactgcttc gagacgccct cgacggagat
     2641 cgctgcgtca gccatcacaa ctcctcgatc acgccgggtg ccgaccgggc gaacagcagc
     2701 cacccgccac acgccaccac gagcgaaacg acactcatca cggcaagatt ccccagagag
     2761 ggcgcttcca gcgtgtacaa cgccttacgc atgttcgtca cgaactgcgt catggggttc
     2821 aactcgaaga cccgacggat cgggaaccat ccgcccagct tctcgggaat gatctcggcc
     2881 cgatagacga tgggcgtcgc gtagaaaagc aactgcagtc cgatcccgat gaggtagctc
     2941 acgtcgcgat accgaacatt ccacaggctc acaacgaggc cgattccgat cgagaacatc
     3001 gccaactgca caatcgtcac gagcgcgaac accgcagtcc acgagacgac tcccaggccg
     3061 atcaggaacg ccagcgcgat acacagctca attccgaact ggaccaacgc cacgcacatg
     3121 ttcgcgatcg ctgggcattc cggagggaag tacaccttgg tgagcatgcc gccggacgct
     3181 tggaaggcca tcatcgagcc attgataacc gagttgaaga agttccaggc gatcagagcg
     3241 cagaacaggt acaacgcgaa gaccttggtg gaaccatcgc cagcgacggg cggctcgacc
     3301 ttcaggaaga cgccgaacac aagcgtgtag ataccgagcg tcgctgccgg gttgatgagc
     3361 gaccacagcc agcccaggat gctcttcttg tgacgagcct tgaggtcccg ctgagcaagg
     3421 tggtagatga ggttccggta ctgccagata cgcaccggag agttggagac gaccatcagc
     3481 tcctcaccgc aggacggcgt tcattcaaaa cgtaggatcc cgggattccg aagtcgctcg
     3541 ggttgaacac cagattcggg ttgtagaacg gatcgtttcc gatgtagtgg ctccaccgtt
     3601 cattgaagtc acgggtctcc accggcgaga cgttgtgatc gcggctcgcg gactcgtgat
     3661 gcaccagctc gacctcgggg ttgaacgcga cccggtaccc ggtctcgcga agcttgaggc
     3721 aataatcgac gtcgttgtag gaggacggaa actgctcgct gaatcctccc acctcgaaga
     3781 acgcgtcgcg tcgcgtcatc atcgctgccg cagtcactgc taggtagttg ttccccagcc
     3841 gtagtaccga gaatgaaccc ggcgcatcac cggggaagcc catgcagaca tgtgccggcc
     3901 cggactcggc attggccgtg gtcacgccgg cgtgttgcag gcgtccgtcg ccgtagacga
     3961 gtttcacgcc taccgcgccg atggcctcct gggcggagta catcagcatc gactcgatcc
     4021 agttgggcgt cgtgatctca atgtcgtcgt tcaacaacag gaggtgatct ccacgcgagt
     4081 tcaggactcc gaggttgatc tgctcggaga agttgaacgg cttgtcgaac tcgacccacg
     4141 tgagtcggcc gccgaagcgt tcctcggcga ccgcccgcac ccgcgggtcg gtgtgaatgt
     4201 ccgcgaccac gacgacctcg tagttctcgt aggtcgagac ctcgtcgatc gtgcgaaggc
     4261 aagccgtcgc caccggctcg accgccccgc gaatcaactt ggcgctcccg gccgtcggaa
     4321 tcacgatgct gacaaggggc gactgcgtca ggcgcggttc gaggcggtac caaccgggtt
     4381 gggccaaatc gtgggtgaca tgggcctcga acccggtccg gtcgcaatgc gactggatgg
     4441 ccttctggcc ggcgatccat gcgtacggtt tggcgttgac atcacctgct gccgaaccgc
     4501 tgatgatgcg ccacccatac aggatctcgg gaatgtggac gatccgcctc gcccgctcgg
     4561 tgacgcgaaa gaccaggtcg aagtcctggc tgccgttgta ttcggcccgg aatccgccga
     4621 cctcgcgcac gagcgatgcc cgcatcgtgg cgaaatggca ggtgtacatc tggtagcgga
     4681 agcgctcggg agaccagtcc ggcttgaaga acgggttgtg gcgaaagccg tcctcggtca
     4741 gcttgtcctc atcggtgtag atgtagtcga tgtcctcgta gtgggcgtcg atcaccgccg
     4801 agaccttgg
//
LOCUS       DAOOMA010000132         4807 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_21281_length_4807_cov_3.696970, whole genome shotgun sequence.
ACCESSION   DAOOMA010000132 DAOOMA010000000
VERSION     DAOOMA010000132.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 4807)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4807)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4807
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21281_length_4807_cov_3.696970"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..727
                     /locus_tag="PLP95_04900"
     CDS             <1..727
                     /locus_tag="PLP95_04900"
                     /inference="COORDINATES: protein motif:HMM:NF013079.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HPB45174.1"
                     /translation="PTVCLVGKGLTFDSGGLNIKVGDFMTNMKNDMGGGAAVYGAMKV
                     AAELQLPVHLVGLVPSAENMPSGRSFRPGDVVTTLSGSTIEVLNTDAEGRVILSDGLH
                     YAQRYNPAAIVELSTLTGAAIIALAHHATGLMSTDQSLADALLAAGESAGDRAWQLPL
                     WEEYREMVKSEIADVKNLAGPAGGSITAGAFLAHFAGDVPFAHLDIAATGWLDKPVKP
                     YQAAGATGAGVRIVAQYLRDLVG"
     gene            779..2446
                     /locus_tag="PLP95_04905"
     CDS             779..2446
                     /locus_tag="PLP95_04905"
                     /inference="COORDINATES: protein motif:HMM:NF015746.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015558 - secondary active
                     p-aminobenzoyl-glutamate transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1902604 - p-aminobenzoyl-glutamate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AbgT family transporter"
                     /protein_id="HPB45175.1"
                     /translation="MNDTAAVAPEGESSGFLDKIETIGNKVPHPVIMFLYLIAGVAVL
                     SMVLSIFDVSVTEEVVTPVPKAELHDLQGQLGGSIVAWDLLTGQEAELPDYTVQEVTY
                     PVRNLLSIDGIRYILSTFVNNFAGFAVVAVTFVAMAGVGVAEQAGMMDALIRRLVSVA
                     PSGAIAFSLIFIGVLSSVASDAGYLILIPLSAAAFHSLGRHPLAGLAAAYAGVGCIFG
                     VNLLITPVDSMLTEITNEAIGSSGEHLDVTANLYFGIVSSFLLAAVAVLVTQRIVERR
                     LGAYDAAEGDPAYVGGGAASGDEVDEEAERRGLKFAFRAMVAMLLAIVVLVAPPGAPL
                     RHPVTDAIIGNTPFMASLIFLITLVFLACGIAYGIGAKTITASDDVIAAVTKTFAGLS
                     GLIFMLLMISQFIAYFNYTNIPRVLAVVLAGVLESANVGALPLLIAMILVILLLDFIL
                     PGVVPKWAIFAPVFIPIFMRLGVAPQTVLAAYRVGDGPVNVMTPLMVYLPFIVTVAQR
                     YQRKAGMGTIVALMLPYVVIISVAWILLFGVFWVFGIPLGPGAPISL"
     gene            2471..4195
                     /locus_tag="PLP95_04910"
     CDS             2471..4195
                     /locus_tag="PLP95_04910"
                     /inference="COORDINATES: protein motif:HMM:NF021588.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta-hydrolase family protein"
                     /protein_id="HPB45176.1"
                     /translation="MTENEFETEDTSSNSNGSHRAQHSFGGWVRWLVRPRLTFGGLVV
                     GLWFALWSLSPSLLPRGWVTQSVVTGIAAVCGYGIGSGLSAALRRWVVRREPSPLVKR
                     RAWMALGAISVIGLATMLWKSRGWQDELRTKVGLEPDPALTRAYVVVVAAVVAVLVLF
                     ISRLVRSGTRALIRPLNRVVPPRVSMAVGSFIMLLLIVGFAQGVLARLAVSTMQSMFS
                     TIDHSTREGMYQPTNAERSGGPGSVVDWDELGFQGRNFIGSGPRLAELESFGGDDCCE
                     EPIRVYVGLAADDSLAERARLAVEELERTGAFEREVLVLFTATGTGYINPYAADAIEY
                     LYGGDTALVAMQYSYLPSWITVLIDDKPPGDTTRAMWDAVMEHLDEMDPGDRPTVLGF
                     GESLGSFGLERGLETVEEIQTLSDGALLIGPTYDNPLRQELTRHRVEGSPEWQPKPSV
                     PGVFFAQEPADLPGPPSADDPPRIVYLQNASDPVTWWNWGLWALEPDWNERPYAVDRS
                     PAFGWRPVVAFWQIVGDLPGAGEVPAGHGHIFGENVVDGWLAVRPPEGWVEADTARLR
                     DLLRDNLG"
     gene            4223..>4807
                     /locus_tag="PLP95_04915"
     CDS             4223..>4807
                     /locus_tag="PLP95_04915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB45177.1"
                     /translation="MTDLSCGLSAASLNAAATAGWYSAVAGLLSGFALLAILIPLDHT
                     DDDCDSNDLSAGAEVFTAAFFTLLILSLTYARLAGETGADVDRGRLTHEMMLNGAALS
                     MSALLLLFALRSILGAYSAARALFVRARGLILIATAVVGPVIALSSQFASSLDLERYR
                     IAAGRTTCEGALPAGVAINLTILLAGWAIIAGLWW"
BASE COUNT          714 a         1526 c         1631 g          936 t
ORIGIN      
        1 cccgaccgtg tgcctcgtcg gcaagggtct gaccttcgac tcgggtggtc tcaacatcaa
       61 ggtcggcgac ttcatgacca acatgaagaa cgacatgggc ggcggagcgg cggtctacgg
      121 ggcgatgaag gtcgccgccg aactgcagtt gccggtccac ctcgtcgggc tcgttccgtc
      181 ggccgagaac atgccgagcg gccggtcctt ccgcccgggc gacgtcgtca cgaccctgtc
      241 ggggtcgacc atcgaggtgc tcaacaccga cgccgaaggc cgggtgatcc tgagcgacgg
      301 tctgcattac gcacagcgct acaaccccgc ggcgatcgtg gaactgtcca cgctcaccgg
      361 cgccgcgatc attgcgctgg cccaccacgc gaccgggctc atgtcgaccg atcagagcct
      421 tgccgacgcg ttgctcgccg ccggcgagtc cgccggcgac cgcgcctggc agctcccgct
      481 gtgggaggaa taccgcgaga tggtcaagag cgagatcgcg gatgtgaaaa acctcgcggg
      541 gccggccggc ggttcgatca ccgcaggggc tttcctcgct catttcgccg gcgacgttcc
      601 ctttgcccac ctcgacatcg cagcgacggg ttggctcgac aagccggtca agccgtacca
      661 ggcggccgga gcgaccggag ccggcgtgcg catcgtcgcc cagtacctcc gcgatctggt
      721 cggctgaccg agacgtcggt gatcaggccc taacactgtc aggcacagga gcccagcaat
      781 gaacgacaca gcggcagtgg cccccgaagg cgagtcctcg ggcttcctcg acaagatcga
      841 gacgatcggc aacaaggtcc cgcacccggt gatcatgttc ctgtacctca tcgccggtgt
      901 ggcggtgttg tcgatggtgc tgtccatctt cgatgtgtcg gtcaccgagg aagtcgtgac
      961 gccggtcccc aaagccgaac tccacgatct ccagggccaa ctcggcggct cgatcgtcgc
     1021 gtgggatctc ctgaccgggc aggaagccga acttcccgac tacacggttc aggaagtcac
     1081 ctacccggtt cggaacctgt tgtcgatcga tgggatccgc tacatcctca gcacctttgt
     1141 taacaacttc gccgggttcg cggtcgtcgc cgtgacgttc gtggcgatgg cgggcgtcgg
     1201 ggtcgccgag caggcgggga tgatggatgc gctcatccgt cgactggtct cggtggcgcc
     1261 gtcaggagcg attgccttca gcctcatctt catcggtgtg ctctccagcg tcgcatccga
     1321 tgcgggctac ctgattctga tacctctatc tgccgcggca ttccattcgc tcgggcgaca
     1381 cccgctggcg ggtctcgccg cggcctacgc cggagtcggg tgcatcttcg gggtgaacct
     1441 gctcatcacc ccggtcgact cgatgctgac cgaaatcacc aacgaggcga tcggttcgtc
     1501 cggcgaacac ctcgacgtga cggccaacct gtatttcggc atcgtgtcgt ccttcctgct
     1561 cgcagcggtg gcggtgctgg tgacccagcg gatcgtggaa cgtcgcctcg gcgcctacga
     1621 cgcggctgag ggcgacccgg cctacgtcgg cggcggcgca gcatccggcg acgaggtcga
     1681 cgaagaggcc gagcgccgcg ggctcaagtt cgccttccgg gccatggtcg cgatgcttct
     1741 cgcgatcgtc gtgctggtcg cacccccggg tgcgcccctg cgccaccccg tcaccgacgc
     1801 catcatcggc aacacgccgt tcatggcgag cctgatcttc ctgatcaccc tggtgttcct
     1861 cgcctgtggc atcgcctacg gcatcggcgc caagacgatc acggcaagcg acgacgtgat
     1921 cgcggcggtg acaaagacct tcgccgggct gtccggcctg atcttcatgt tgctgatgat
     1981 ttcccagttc atcgcctact tcaactacac gaacatcccc cgggtcctcg cggtggtgtt
     2041 ggccggcgtg ctcgagtcgg cgaacgtcgg tgcgttgccg ttgctcatcg ccatgatcct
     2101 cgtgattctc ctgttggact tcatccttcc cggggtcgtg ccgaagtggg cgatctttgc
     2161 cccagtgttc atcccgatct tcatgaggct cggcgttgcg cctcagacgg tgctggcggc
     2221 gtaccgagtt ggggacggcc cggtcaacgt catgaccccg ctcatggtgt atttgccgtt
     2281 catcgtgacg gtcgctcagc gctatcagcg caaggccggc atgggcacca tcgtggcgtt
     2341 gatgttgccg tacgtggtca ttatttcggt ggcgtggatc ctgttgttcg gcgtgttctg
     2401 ggtctttggg atcccgctcg gtcctggagc ccccatctcc ttgtgatcta ctgatcacag
     2461 aggagtgggt gtgactgaga acgagttcga gaccgaggac acgtcgagca acagcaacgg
     2521 gagccaccgc gcccagcatt ccttcggggg ctgggtgcgg tggttggttc gtccgcgcct
     2581 gacctttggc gggctggtcg tcggtctgtg gttcgcgttg tggtcgctca gcccgtcgtt
     2641 gttgccgcgc gggtgggtca cccagagcgt ggtcaccggc attgccgcgg tgtgcgggta
     2701 tggtatcggg tcgggcctca gcgcagcgtt gcggcgatgg gtcgtacgac gcgagccgtc
     2761 accgctggtc aagcggcgcg cctggatggc cttgggtgcg atctcggtca tcggtttggc
     2821 gacgatgctt tggaaatcgc gcggatggca ggacgaactg cgcaccaagg tcggcctgga
     2881 gccggacccg gcgctgacgc gggcgtatgt ggtcgtggta gccgccgtgg tggcggtgct
     2941 ggtgttgttc atctcccgct tggtgcgctc gggaacccgg gcgttgatcc gtccactgaa
     3001 ccgggtcgtc ccgccacggg tgtcgatggc ggtcggatcg ttcatcatgt tgttgctgat
     3061 cgtcgggttc gcccaggggg ttctggcccg gctcgccgtg tcgaccatgc agtccatgtt
     3121 ctccacgatc gaccactcga cacgcgaggg catgtatcaa ccgacgaatg ccgaacgctc
     3181 cggagggccg ggttcggtgg tggactggga cgagttggga ttccaaggcc gcaatttcat
     3241 cgggtccgga cctcggctcg ccgagttgga aagcttcggt ggtgacgact gctgcgagga
     3301 gccgatccgg gtgtacgtcg ggttggccgc cgacgattcg ctggccgaac gggcccgcct
     3361 cgcggttgag gagctcgagc gaaccggagc gttcgagcgc gaggtcctgg tgttgttcac
     3421 cgctacgggt accggataca tcaaccccta tgcggcggat gccatcgagt atctgtacgg
     3481 cggcgacacg gcgctggtgg cgatgcagta ttcctacctg ccgagttgga tcaccgtgct
     3541 gatcgacgac aagccaccgg gcgacaccac ccgggccatg tgggacgcgg tgatggagca
     3601 tctcgacgag atggaccccg gcgaccgccc gacggttctg ggattcggag aaagcctggg
     3661 ctccttcggg ttggagcgcg ggctcgagac cgtcgaagag atccagacgc tcagcgacgg
     3721 ggcgttgctc atcggcccga cctacgacaa cccgttgcgt caggaactga cccgccatcg
     3781 ggtcgagggt tcgcccgagt ggcagccgaa gccgtcggtg cccggggtgt ttttcgccca
     3841 ggagcccgcc gatcttcccg ggccaccgag cgcggatgat ccgccgcgaa tcgtgtacct
     3901 ccagaacgca tcggatcccg tgacgtggtg gaactggggt ctgtgggcgc tggagccgga
     3961 ttggaacgag cggccgtatg ctgtcgaccg gtcaccggcg ttcgggtggc gtccggtcgt
     4021 ggcgttctgg cagatcgtcg gggacctgcc cggtgctggc gaggtccctg ccgggcacgg
     4081 ccacatcttc ggcgagaacg ttgtcgacgg ctggttggcg gttcggccgc ccgagggctg
     4141 ggttgaggcc gacacggccc ggctgcgcga cctgctgcgc gacaacctgg gatagggcga
     4201 cggtcgacgt gggcgcttcg acatgaccga cctcagctgt ggactgagcg cggccagcct
     4261 gaacgcagcg gcgaccgctg gctggtattc cgcggttgcc gggctcctga gcgggtttgc
     4321 gctgctggcg attctgattc cgctcgacca caccgacgat gactgcgaca gcaacgatct
     4381 gtcggccggg gcagaggtgt tcaccgctgc cttcttcacg ttgctcatct tgagcctcac
     4441 ctacgcgcgg ctcgcgggcg agacaggcgc cgacgtcgac cgaggccgcc tcacacacga
     4501 gatgatgctc aacggtgccg cgctgtcgat gtcggcgttg ttgttgctgt ttgcgttacg
     4561 cagcatcctg ggggcctaca gcgcggcacg ggcgctcttc gtgcgagcga gagggctgat
     4621 cctcatcgct actgcggtcg tgggcccggt gattgcgctg tcctcgcagt tcgccagctc
     4681 cctcgacctc gagcgctacc gcatcgccgc aggccgtacc acctgcgagg gagcgctgcc
     4741 ggccggtgtg gcgatcaatc tcaccatcct gctcgcgggt tgggcgatca tcgccgggtt
     4801 gtggtgg
//
LOCUS       DAOOMA010000133         4784 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_21437_length_4784_cov_4.113343, whole genome shotgun sequence.
ACCESSION   DAOOMA010000133 DAOOMA010000000
VERSION     DAOOMA010000133.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 4784)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4784)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4784
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21437_length_4784_cov_4.113343"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            285..878
                     /locus_tag="PLP95_04920"
     CDS             285..878
                     /locus_tag="PLP95_04920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HPB45178.1"
                     /translation="MANPLPISELSGGFDPISDVSSKLLKNRIVILGSEVNDQVANLI
                     SAQLLYLDGQDDSSDIWLYINSPGGSVTAGLAIYDTMNFIKPNVGTVCMGLAASMGQF
                     LLSAGAKGMRFALPSSRIMMHQPSGGFQGNAADIKIQAEQMIYIKQMLSERIAEHTGQ
                     TVDQIIKDSDRDRWFTAPEAKDYGIIDQVIVRRGEMH"
     gene            complement(1154..1597)
                     /locus_tag="PLP95_04925"
     CDS             complement(1154..1597)
                     /locus_tag="PLP95_04925"
                     /inference="COORDINATES: protein motif:HMM:NF012415.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HPB45179.1"
                     /translation="MHATIRTTSTSRRTVALVLTTLIALLASTGCDRAMAGRSQTLAN
                     EARNARGTHSLVWDEQAATKAQAWANHLASQGSLAHSRLTDGMSGYSALAENVGYASS
                     VEAVHDLFMNSSQHRKNILGGQYTSIGVGVAQANGSYYVVQVFKG"
     gene            complement(1688..2116)
                     /locus_tag="PLP95_04930"
     CDS             complement(1688..2116)
                     /locus_tag="PLP95_04930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB45180.1"
                     /translation="MKPRRPFATRTIAAAASFLLVGLTASACTSDTDRFCNDLRDIES
                     LSSLITAIERRDERSITNGLDQLRKLQDIAPSEIFDDFRALVDALSATVQITLNTPDE
                     MGTTGQVDATELTAQLEKIDAPAHNVAEFAEENCGISLNP"
     gene            complement(2124..2825)
                     /gene="rpe"
                     /locus_tag="PLP95_04935"
     CDS             complement(2124..2825)
                     /gene="rpe"
                     /locus_tag="PLP95_04935"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HPB45181.1"
                     /translation="MISTPGTDRPIQIAPSVLPANFARLGEECQALEKAGVDRIQWDV
                     MDGVFVPNLTFGPDVIASVRDLVSVPFEAHLMVVEPDKLMGRYIDAGCEIVIVHAEAC
                     LHLHRTLDAINKAGAKAAVALNPATPASAIRHVMDLLDMVLVMTVNPGFGGQAYISTM
                     EPKVAEIRGWVVEEGYDCDIEVDGGVGPATASGPASAGANVLVAGSALFNHPGGLAAA
                     TTEIRAIGQAAAATR"
     gene            2882..3715
                     /locus_tag="PLP95_04940"
     CDS             2882..3715
                     /locus_tag="PLP95_04940"
                     /inference="COORDINATES: protein motif:HMM:NF018076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HPB45182.1"
                     /translation="MESLEFVVEADEVEFACGLCWSLGVAGIEERDEPDGRVRLLVGV
                     ESDAADDAVDELGTRWDVTRSEMNVEAYLDAWRPFARAVRVGERLVIQPPWVDPIARP
                     GDLVVSVDPGRAWGHGAHPTTVLCVEAILRIGDLDGWRVLDVGCGSGVLSVASALLGA
                     KAVVGIDVDFAAVDATLANALVSGVPELVEASTTPVGSIVETFEIVLANIGLGTLCEL
                     AGPIGARVAANGILILSGLLVEQVDDALEAYGDFEPVWRRDLDGWSTVALRRRSFRVG
                     R"
     gene            complement(3758..>4784)
                     /gene="serB"
                     /locus_tag="PLP95_04945"
     CDS             complement(3758..>4784)
                     /gene="serB"
                     /locus_tag="PLP95_04945"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036424 - L-phosphoserine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoserine phosphatase SerB"
                     /protein_id="HPB45183.1"
                     /translation="TRDLESMGVDRGLQISVAATDPTPTRRDHGMVVTVVARSITSTH
                     FNAVARAIATAGGNIDRIVRLARYPVSAYEIQVQRGDEEALRTQLVAVSREFHIDVAI
                     QPGGLARRAQRLVVLDVDSTLIQDEVIELIAELCGCGEQVRDITERAMAGELDFEQSL
                     RERVRLLKGQRCEDLATALGRVRLTPGARTFTRTLRRLGFRIAIVSGGFTYFTDALKA
                     DLELDYAFANELEVVDGRLTGEVVGPVVDRARKAELLRHVAELEGIALDQTVAVGDGA
                     NDLDMLATAGLGIAFNAKPVVRDAAAATLNVPYLDAVLFMLGVSRGEVEAADAAEGIV
                     EEPPPVN"
BASE COUNT          801 a         1453 c         1656 g          874 t
ORIGIN      
        1 gcgttctcgc cgcgacggaa agcatctgaa tcgatttgag atgcaccggc gtagtcgtac
       61 atatcgccat agtatcgaac aggcgtacga tacgcaagtc gaaatgcgga agaaccaaac
      121 aaaagttcga tccgaagtgt gggcgtcgtc tgggtgcaag ggcagcgcag gcagcaaggg
      181 cagcacaggc cccggcggcg cggaggtgct ctcgcgccgc ccgcgttggc tcgttcgctc
      241 gaccggtgcc ggaatcgtca aggtgccgac taaccttcac tccaatggct aacccacttc
      301 cgatctccga actgagcggc ggcttcgacc cgatctccga cgtgtcgagc aagctgctga
      361 agaaccgcat cgtgatcctc ggctccgagg tcaacgatca ggtcgcaaac ctgatctcgg
      421 cgcagctcct gtacctcgac ggacaggacg acagctccga catctggctg tacatcaaca
      481 gcccgggcgg atcggtcacc gccggtctgg ctatctacga cacgatgaac ttcatcaagc
      541 cgaacgttgg cacggtctgc atgggactcg cggcgtcgat ggggcagttc ctgctgtcgg
      601 cgggagcgaa gggcatgcgg ttcgcgttgc cgagcagtcg gatcatgatg catcagccct
      661 ccggcgggtt tcagggcaac gcggccgaca tcaagatcca ggccgagcag atgatctaca
      721 tcaagcagat gctgtccgag cgcatcgccg agcacaccgg ccaaaccgtc gatcagatca
      781 tcaaggactc cgatcgcgac cgttggttca ccgctcctga ggcgaaggat tacggcatca
      841 tcgaccaggt gatcgtgcgc cgcggcgaaa tgcactgagt ttccttggcg gggccagggc
      901 cggcggttgg cgcgcccgta ccccgcgagc tgccgcatcg cagacggcac tcgcgctgca
      961 ctcgcgctgc gcatcgcaga ccggcagttg cgactccatg acggacgtgt tgcgacgcga
     1021 gtgttgcaat agtcacgatt gaatgccggg tcgaatattg caaacgtcaa acggcccgct
     1081 cgagatgagc gggccgtttc atgtcatttc gacgagttgt gtcgggtcgg tggcccggcc
     1141 gggccgccgt ggctcagccc ttgaagacct ggaccacgta ataggagccg ttggcctggg
     1201 cgacgcccac cccgatggag gtgtactggc caccgaggat gttcttgcgg tgctgggagg
     1261 agttcatgaa cagatcgtgc accgcttcga cgctcgacgc gtagccgacg ttttctgcca
     1321 atgccgagta gccggacatc ccgtccgtca ggcggctgtg ggctagcgac ccctgtgatg
     1381 caaggtgatt cgcccaagcc tgggccttgg ttgcggcctg ttcgtcccac accagcgagt
     1441 gcgttcctcg cgcattgcgg gcttcgttgg ccagcgtctg tgagcgaccc gccatggccc
     1501 ggtcgcagcc tgttgaggca agtagtgcga tcagggtggt gagcaccaac gcaacggttc
     1561 ggcgtgatgt tgacgtggtg cggatggtgg cgtgcatgct gtgattgctc ccctcagctc
     1621 gccctagtct ggcgtgtaaa cactctacca cgctattgca atagtgttgc aattatgtga
     1681 cggaatgtta cggattgagc gagattccac agttctcttc ggcaaattcc gcaacgttgt
     1741 gagccggagc gtcaatcttt tcgagctgag cggtgagttc ggtcgcatcg acctggcccg
     1801 ttgtacccat ctcgtcgggg gtgttcaggg tgatctgcac cgtcgcactc aacgcgtcca
     1861 cgagggctcg gaaatcgtcg aagatctcgg acggcgcgat gtcttgcagc ttgcggagct
     1921 ggtcgaggcc gttggtgatg ctgcgctcat cacgccgttc gatcgccgtg atgaggctcg
     1981 acaggctctc gatgtctcga aggtcgttgc agaaccggtc cgtatcgctg gtgcatgcgg
     2041 acgcggtcag gccgacgaga aggaacgagg cggcggccgc aatcgtgcgc gtggcgaagg
     2101 gacgacgagg cttcaccggt tcgttatcga gttgccgcgg cggcttggcc gatcgctcga
     2161 atctcggtgg tcgccgcagc gagcccgccg gggtggttga acagggcgct gcccgcaacc
     2221 aggacgttcg cgccggcact cgccggcccg gatgcggtgg ccggcccgac cccgccgtcg
     2281 acctcgatgt cgcagtcgta gccctcctcg accacccagc cgcgaatctc ggcgaccttc
     2341 ggctccatgg tggagatgta ggcctgccca ccgaaaccgg gattcaccgt catgaccagg
     2401 accatgtcga gcagatccat gacgtggcga atggccgagg ctggggttgc ggggttgagc
     2461 gcgaccgccg ccttggcgcc ggccttgttg atcgcgtcga gggtgcggtg caggtgaagg
     2521 caggcttcgg cgtgcacgat gacgatctcg cagccggcat cgatgtagcg gcccatcaac
     2581 ttgtcgggct cgaccaccat caggtgggcc tcgaacggca cgctgacgag atcgcgcacc
     2641 gacgcgatga catcggggcc gaaggtgagg ttcgggacga acacgccgtc catcacatcc
     2701 cattggatcc ggtcgacacc cgccttctcc aacgcctggc attcctcacc gagtcgggcg
     2761 aagttcgctg gcaacaccga gggggcgatc tggatcggtc ggtcggttcc gggcgtggag
     2821 atcatggggg ccacgctagt tggctgcgat ggcaggggcg ttcgcgccgt agcctcaggt
     2881 gatggaatcg cttgagttcg tggtcgaagc ggacgaggtg gagttcgcct gtggcctgtg
     2941 ctggtcgttg ggagtcgccg ggattgagga gcgcgacgaa cccgatggcc gggtgcgtct
     3001 gctcgtgggg gtcgaatccg acgcggccga tgacgccgtc gacgaactcg ggacccgttg
     3061 ggatgtcacg cgcagcgaga tgaacgtcga ggcgtacctc gatgcgtggc gcccgttcgc
     3121 ccgtgcggtg cgagtggggg agcggctggt gattcaaccg ccatgggtgg acccgatcgc
     3181 tcgccccggc gacctggtcg tgtcggtcga tcccggacgt gcctggggcc atggagcaca
     3241 tccgacaacc gtcctgtgcg tcgaggcgat tctgaggatc ggagacctcg acgggtggcg
     3301 ggtgctcgac gtcggctgtg gcagcggcgt gctgagcgtg gcgagcgcgt tgttgggggc
     3361 gaaggcggtg gtcgggatcg acgtcgactt cgcagcggtc gacgcaacgc tcgcgaatgc
     3421 cctggtcagt ggcgttcccg agttggtcga agcctccacg accccggtgg gttccatcgt
     3481 cgagaccttc gagatcgtgc tcgcgaacat cggcctgggg acactgtgcg agttggccgg
     3541 accgatcgga gctcgcgtcg ctgccaacgg gatcttgatc ctgagcgggc tgctcgtcga
     3601 acaggtcgac gatgcgctcg aagcctacgg agacttcgaa ccggtatggc ggcgggatct
     3661 cgacggttgg agcactgtgg cgttgcggcg gcgcagcttc cgcgtcggac gttgatcgcg
     3721 ccggcagccg ccggcagccg ccgtgggacg tgtccgttca gttcacgggc gggggttcct
     3781 cgacgatgcc ctcggccgca tcggcggcct cgacttcgcc gcggctgaca cccaacatga
     3841 acagcacggc atcgagatag gggacattca aggtcgccgc cgcggcgtct cgaacgaccg
     3901 gcttggcgtt gaatgcgatc cccagcccgg cggtggccaa catgtcgagg tcgttcgcgc
     3961 cgtcgccgac ggccacggtc tggtcgaggg cgataccttc gagttcggcc acgtggcgca
     4021 gcagttcggc cttccgcgcc cggtcgacca ccggaccgac gacctcgccc gtcaaccggc
     4081 cgtcgacgac ctcgagctcg ttggcgaacg cgtaatccag ttcgaggtcg gccttgagcg
     4141 cgtcggtgaa ataggtgaat ccgccggaga cgatcgcgat gcggaaccca aggcgtcgca
     4201 aggtgcgggt gaaggtgcgg gctccggggg tgaggcggac tcggccgagc gcggtggcca
     4261 ggtcttcgca acgttgcccc ttgaggagcc ggacccgctc gcgtagggac tgctcgaaat
     4321 cgagctcgcc ggccatcgcc cgttcggtga tgtctctgac ctgttcgccg cagccgcaca
     4381 gctcggcgat gagttcgatg acctcgtctt ggatgagggt cgagtcgacg tccagcacca
     4441 cgagccgttg cgctcggcgt gccaggccac cgggctggat ggcgacgtcg atgtggaact
     4501 cccgtgacac ggccacgagc tgggtccgaa gcgcctcctc gtcgccgcgt tggacctgga
     4561 tctcataggc cgaaaccgga tagcgcgcca ggcggacgat gcgatcgatg tttccgccgg
     4621 cggttgcgat ggcccgggcg acggcgttga aatgcgtcga ggtgatcgag cgggcgacca
     4681 cggtgacgac catgccgtgg tcgcgtcgcg tcggcgtggg atcggttgct gcgacgctga
     4741 tctgcagccc acggtcgacg cccatggact cgaggtcacg ggtg
//
LOCUS       DAOOMA010000134         4782 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_21450_length_4782_cov_3.744447, whole genome shotgun sequence.
ACCESSION   DAOOMA010000134 DAOOMA010000000
VERSION     DAOOMA010000134.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 4782)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4782)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4782
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21450_length_4782_cov_3.744447"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..947)
                     /locus_tag="PLP95_04950"
     CDS             complement(<1..947)
                     /locus_tag="PLP95_04950"
                     /inference="COORDINATES: protein motif:HMM:NF012488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HPB45184.1"
                     /translation="MLTTLVGDDVTVPCLDGVERTYLCLDAGASTPAFQAVADAVTGF
                     LPWYSSVHRGAGAKSQFATRRYEEARDAVMRWVGRSDTDDIAVLCRNTTEAINIAAHR
                     IPLGPNDTIVTTVVEHHANLLPWQRAATRSGATISYVECGRDGTFSPDDVAAAVNATV
                     AEGRRPAVVAITGAANVTGWMPDLDPIIDHAHQHGALVLVDAAQLAPHRPLHTVRADF
                     IAWSGHKMYAPFGSGVLIGPRRVFEHGDPFLVGGGAVNLVDLDGAMWTEAPDREEAGS
                     PNVVGAVALHAAIDTFETLRWEAIVEHELAMAKALRHGLA"
     gene            1171..2271
                     /gene="ald"
                     /locus_tag="PLP95_04955"
     CDS             1171..2271
                     /gene="ald"
                     /locus_tag="PLP95_04955"
                     /EC_number="1.4.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000286 - alanine dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine dehydrogenase"
                     /protein_id="HPB45185.1"
                     /translation="MPREVKDSEDRVGLTPDGVDELVRAGARVLVEASAGEGIGVHDE
                     EYRGAGASIVGVDAVWEDSDLVIKVKEPQPAERKRLHHGQTLFAYLHLAPDPQQAQDL
                     LASGAACIAYETVTDAAGALPMLAPMSQVAGRMSVQAAAHFLEGPHGGSGRLLGGVPG
                     VPAAKVVIIGGGTAGENAASIAVGMGADVTVLDRNPAVLDHLDRRFGPAISTQYSTGG
                     AIDRLVVGADVVIGAVLIRGARAPRLVTRDHVARMRPGSVIVDIAIDQGGCCETSRPT
                     THSEPTYVVDGVVHYCVTNMPGGVAATSTAALTHATLAAAVALVRFGTEEALRRDPHL
                     RDGLNVYRGRITDAAVAEALGLSYVDPLEALA"
     gene            complement(2406..3344)
                     /locus_tag="PLP95_04960"
     CDS             complement(2406..3344)
                     /locus_tag="PLP95_04960"
                     /inference="COORDINATES: protein motif:HMM:NF012517.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HPB45186.1"
                     /translation="MQISIWPSSERPWGEVRDDVVRADSLGWDGVWLADHFMPNAPVD
                     DSAGRRGPYLEAWSCLGALAALTSNVRLGSLVSSVTYRHPAVLAAIACTVDEISTRPH
                     GSGRVVLGIGAGWQENEHEAYGLTLGSLRERSDRLAEAATVLRSLLSDERTTFAGRFF
                     QLDDAPNEPRPTGRPIPLLIAGKGELRTIPTAARLGDEWNAWASPETFAHKYRVLCDA
                     AEAAGRDIRSMRCSTQAFVVFGDRPEVVESITSMGRPVVSGSDAQILDVLATYRSAGV
                     DEFIVPDWLWDDATERAEHYHHFYEVTRPLHVEGGP"
     gene            3459..4205
                     /locus_tag="PLP95_04965"
     CDS             3459..4205
                     /locus_tag="PLP95_04965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB45187.1"
                     /translation="MPGRGRKSVVARRVAAAASVAVALVVTTPELTKASPSRPSPYAE
                     VVVPAGAHHPLANEASRALGAESVDPPAPDAVPQLGLIETDTDPASTPAPPTAPTTTL
                     APRPVGDRVLAAIAFPWQAYLPGWTIEFVGPRRGFRGITYTAKRRIEIFVRPDLGFEE
                     LVHVTAHELGHALDVTLLDDEQRDWWMHYRGGTESWWVSDGASDFASGAGDWAECFAW
                     WQTQRGGFFSEVAGPPDVASIQLMADMIGG"
     gene            4262..>4782
                     /locus_tag="PLP95_04970"
     CDS             4262..>4782
                     /locus_tag="PLP95_04970"
                     /inference="COORDINATES: protein motif:HMM:NF013226.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiA family prenyltransferase"
                     /protein_id="HPB45188.1"
                     /translation="MANVASASPNESVPNSPHPGPGAVLRGAVRACRPKQWAKNALVF
                     IAPVAANAIVSKGPDGIEFHGRPFLQTMLAFVVFSMVASATYLINDSVDVEADRRHPT
                     KRNRPIAAGIITVRLAVALAIVLGVAGLALAYWRSPQFCVVVAIYAVQTTLYSFWLKN
                     EPVLDLVVLSGGF"
BASE COUNT          741 a         1580 c         1679 g          782 t
ORIGIN      
        1 gcgaggccat ggcgtaacgc cttggccatc gcgagttcgt gttcgacgat ggcctcccat
       61 cggagtgttt cgaaggtgtc gatcgccgcg tgcagggcga ccgctcccac gacgttggga
      121 gaccccgcct cctcgcgatc gggggcctcg gtccacatcg ccccgtcgag gtcgaccagg
      181 ttgacggcgc cgccgccgac gaggaacggg tcgccgtgtt cgaacacccg gcgcgggccg
      241 atcaggaccc cggagccgaa tggcgcgtac atcttgtgcc ccgaccacgc gatgaaatcc
      301 gcccgaaccg tgtgcagcgg acgatggggc gccaactgtg cggcgtcgac gagcaccaac
      361 gcaccgtgtt gatgagcgtg atcgatgatc gggtccaggt cgggcatcca cccggtgaca
      421 ttcgcggcgc cggtgatggc gaccaccgcc ggccgccgac cttctgcaac ggtcgcgttc
      481 actgccgcgg cgacgtcgtc ggggctgaag gtgccgtctc ggccgcactc gacatagctg
      541 atcgtggcgc cgctgcgtgt ggcggcgcgc tgccacggca acagattcgc gtggtgttcg
      601 acgacggtgg tgacgatggt gtcgttcggg ccgagtggga tccggtgggc cgcgatgttg
      661 atcgcctcgg tggtgttgcg gcacaacacc gcgatgtcgt cggtgtcgga gcggccgacc
      721 catcgcatca ccgcgtcgcg tgcctcctca tagcggcggg ttgcgaactg ggacttcgcc
      781 ccggcgccgc ggtgcacact cgaataccag ggcagaaacc ccgtcaccgc atcggcgacc
      841 gcttgaaaag ccggggtcga ggcgccggca tcgaggcaca ggtaggttcg ctcgaccccg
      901 tcgaggcaag ggaccgtgac gtcgtcgccg acgagtgtgg tgagcatggt gtggccttgg
      961 tgattcaacg gtggtgggcg ggacggtcgc aagcttgcac gcgttcgcaa gctcgaaagc
     1021 gttcttttgg ccctccattg tcgcgccgtg cgaggccgga gccgcgcccg gcgcgtgccc
     1081 ggcgcgtcca gtcgcgctcg gttcataaac tcgcacgggt ccgcacaacg gcgtgcgggc
     1141 ggaaagcgag acggtcccgt gatcgtgggc gtgcccaggg aagtgaagga ttccgaggat
     1201 cgcgtggggc tgacccccga cggggtcgac gaattggtcc gggccggtgc ccgcgtcctg
     1261 gtcgaagcct cggcggggga ggggatcggc gtgcacgatg aggagtatcg cggcgccggt
     1321 gcctcgatcg tcggcgtcga cgcggtgtgg gaggactccg acctggtcat caaggtgaag
     1381 gaacctcagc cggccgaacg caagcgcctg catcacggcc agacgctgtt cgcctacctt
     1441 cacctcgccc ccgatccaca gcaggctcag gatctcctcg ccagtggtgc ggcgtgtatc
     1501 gcatacgaga cggtgaccga cgcggccgga gcgctgccga tgctcgcccc gatgtcgcag
     1561 gtggcgggtc gcatgtcggt gcaggcggcc gcccacttcc tcgaggggcc acacggcggt
     1621 tcgggtcgac tgctcggcgg ggttcccgga gtgccggcgg cgaaggtggt gatcatcggc
     1681 ggcggaacag cgggggagaa cgcagcgtcg atcgccgtcg gcatgggcgc cgacgtgacg
     1741 gtcctcgacc gcaacccggc ggtgctcgat catctggatc gacgcttcgg cccggcgatc
     1801 tcgacgcagt actcgaccgg cggggcgatc gaccggctgg tcgtcggggc cgatgtggtg
     1861 atcggtgcgg tgctcatccg gggcgcccgt gcaccgcggc tggtcacccg cgaccacgtc
     1921 gcgcgaatgc gtccgggttc ggtcatcgtc gatatcgcca tcgaccaagg tggctgctgt
     1981 gagacctcgc ggcccacgac gcactccgaa ccgacctacg tggtcgacgg ggtggtgcac
     2041 tactgcgtga cgaacatgcc gggaggggtg gccgcgacgt cgaccgccgc gctgacccac
     2101 gccaccctgg ccgcggcggt ggcgctcgtc cggttcggaa ccgaggaggc gttgcggcgc
     2161 gacccgcacc ttcgcgacgg gctcaacgtg taccgcggaa ggatcaccga cgccgcggtg
     2221 gccgaggcgc tcgggttgtc ctacgtcgac ccgctcgagg ctctcgcttg aaaaccggcg
     2281 cgcatgccca tgccccggcc atgccccgcg cccgcctgtt ctccgataac cagtggtcgt
     2341 gatcacgacc actggttatc ggagaacagg ccggggaggc ggcaggctgt tgcggcggca
     2401 gacggtcagg ggccgccctc gacgtggagg gggcgggtga cctcgtagaa gtggtggtag
     2461 tgctcggcgc gttcggtggc gtcgtcccac agccagtccg gcacgatgaa ctcgtcgact
     2521 ccggctgacc ggtaggtcgc caacacgtcg aggatctggg cgtcggatcc cgacacgacc
     2581 gggcgcccca tcgaggtgat cgactcgacc acctcgggcc gatcgccgaa caccacgaag
     2641 gcctgggtcg agcatcgcat cgaccggatg tcgcgcccgg cggcctcggc ggcgtcgcag
     2701 agcactcggt acttgtgggc gaacgtctcg ggcgaggccc atgcgttcca ttcgtcgcca
     2761 aggcgcgccg cggtcggaat cgttcgcagt tcgccctttc cggcgatcag caggggaatg
     2821 ggtcgacccg ttgggcgcgg ctcgttggga gcgtcgtcga gctggaagaa ccggccggcg
     2881 aacgtcgtgc gttcgtcgct cagcagcgag cgcagcaccg tcgcggcctc ggcgagacga
     2941 tcggagcgct cgcgaagcga accgagggtc aacccgtagg cctcgtgttc gttctcctgc
     3001 cagcctgcac cgattcccaa caccacccgg ccgctgccat gagggcgagt gctgatctcg
     3061 tcgaccgtgc aggcgatggc ggcgagcacc gcgggatggc ggtaggtgac cgaactgacc
     3121 agcgaaccga gccgtacgtt gctcgtcagc gccgccaatg cgccgaggca ggaccacgct
     3181 tcgagatacg ggccacgtcg accagcggaa tcgtcgaccg gggcgttggg catgaagtga
     3241 tcggccagcc agaccccgtc ccagccgagc gaatcggcgc gaacgacatc gtctcggacc
     3301 tcaccccacg ggcgttcgga actcggccag atactgatct gcatgggtgc agcctagaca
     3361 cgcggccagc tcggagaccg tccccctagt agtgcatgac gcaatgagag gcagtgaaga
     3421 actgggagat gcattgacag cctccgtacc atcgccgcat gcctggtcgt ggtcgcaaga
     3481 gcgtcgtggc gcgtcgtgtc gccgcagcgg cctcggtggc cgtggcgctc gtcgtgacaa
     3541 cccctgaact caccaaggcg tcgccaagtc ggccatctcc gtacgcggag gtcgtcgtgc
     3601 cggctggtgc acaccacccg ctggccaacg aagcgagccg ggcactcggc gcagagtcgg
     3661 tcgatccgcc ggcgccggat gctgtgccgc agttggggtt gatcgagacc gacacggacc
     3721 cggcatcgac cccggcaccc ccaaccgcgc ccaccacgac cctggccccg cgtccggtcg
     3781 gggaccgggt gttggcggcg atcgcctttc cctggcaggc gtacctgccg ggttggacga
     3841 tcgaattcgt cggaccgagg cgcggctttc gaggaatcac ctacaccgcc aagcgacgaa
     3901 tcgagatctt cgtgcgcccc gacctgggct ttgaggagct cgttcatgtc accgctcacg
     3961 aactgggcca tgcgctcgac gtcaccctgc tcgacgacga acaacgcgac tggtggatgc
     4021 actaccgcgg tggcaccgag agctggtggg tgagcgacgg ggcctccgac tttgccagcg
     4081 gcgcgggcga ctgggccgag tgtttcgcct ggtggcagac ccaacgcggc ggattcttct
     4141 ccgaggtggc cgggcctccc gacgtcgcat cgattcagct catggcggac atgatcggcg
     4201 ggtgaagact tggtggcgtc acggtcgggt ctgagcatcc tccgttacag tgaacacacc
     4261 catggccaac gtcgcgtccg catcccccaa cgaatccgtt cccaattcac cgcacccggg
     4321 tccgggtgcg gtgcttcgtg gcgccgtgcg ggcttgtcgg cccaagcagt gggcgaagaa
     4381 tgcgctggtg ttcatcgccc cggtcgcggc gaacgccatc gtgtcgaagg gccccgacgg
     4441 catcgagttc cacggccggc cgtttctgca gaccatgctc gccttcgtgg tgttttcgat
     4501 ggtcgcgagt gcgacgtatc tgatcaacga ctcggttgac gtcgaggccg accgtcgcca
     4561 cccgaccaaa cgcaatcgcc cgatcgccgc gggcatcatc accgtgcgtc tcgcggtcgc
     4621 gctcgcgatc gtgctcggcg ttgccggcct ggcgctggcg tactggcgaa gcccgcagtt
     4681 ctgcgtggtc gtcgccatct atgccgtgca gaccacgctg tattcgttct ggctgaaaaa
     4741 cgaaccggtg ctcgacctcg tcgtgttgtc gggcgggttc at
//
LOCUS       DAOOMA010000135         4778 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_21476_length_4778_cov_4.288588, whole genome shotgun sequence.
ACCESSION   DAOOMA010000135 DAOOMA010000000
VERSION     DAOOMA010000135.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 4778)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4778)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4778
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21476_length_4778_cov_4.288588"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            351..779
                     /locus_tag="PLP95_04975"
     CDS             351..779
                     /locus_tag="PLP95_04975"
                     /inference="COORDINATES: protein motif:HMM:NF039728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6325 family protein"
                     /protein_id="HPB45189.1"
                     /translation="MGPLDILIIGFPDGKPDPVGFDRLAALVDAGTIRILDLEFVLRD
                     EGVAYLAKSTDLPPVDGFDADFWAGASSHLLDRDDLDHLAADLEEGELGLVVLIEQSW
                     MLGLIDEWVGNGAHLIADDGLPSRDLVDALDAAERREGSD"
     gene            779..1060
                     /locus_tag="PLP95_04980"
     CDS             779..1060
                     /locus_tag="PLP95_04980"
                     /inference="COORDINATES: protein motif:HMM:NF021378.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SHOCT domain-containing protein"
                     /protein_id="HPB45190.1"
                     /translation="MGLIKTATRVAVASSVHGRVQRRQQERWAAQPPQPPAAPPMPAP
                     HVAPAPPAPAPPAVDLQQQVDLLRQLGELRDAGIIDEAEFAAKKAKILG"
     gene            1111..1509
                     /locus_tag="PLP95_04985"
     CDS             1111..1509
                     /locus_tag="PLP95_04985"
                     /inference="COORDINATES: protein motif:HMM:NF015953.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HPB45191.1"
                     /translation="MVRLLATAVVAFLSNAVGLIVAALILDDVRLGTLGFVIAALIFT
                     GVSVLTEPLLRQAAMKRAPALLGSTALLTVLVSLIVTAIISDSLSINGLVTWVLATIV
                     VWLISFVGRLVFPLLVFKKTLSAARSSRSA"
     gene            1748..2437
                     /locus_tag="PLP95_04990"
     CDS             1748..2437
                     /locus_tag="PLP95_04990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020378467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase cytochrome b subunit"
                     /protein_id="HPB45192.1"
                     /translation="MTSASLKVAMAVSGSVLMAFVFVHMVGNLKVFLGRDELNHYAAF
                     LREFGVPLMPHGWFLWIVRVLLTVCVVTHMASAAVLTSRARAAGRNRTTRGHQVSWWR
                     SFTSRTMAVSGVVITAFVGFHLLDLTLGVAAADGFRHPATVDGATAYFAYENLVGSFQ
                     RPAAAALYVLAMLVLGAHLSHGSWSVIHDLGVTGARARRTALIVLSVVAAAVVVGNVS
                     IPLAVQAGWVS"
     gene            2439..4391
                     /locus_tag="PLP95_04995"
     CDS             2439..4391
                     /locus_tag="PLP95_04995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020378468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase/succinate dehydrogenase
                     flavoprotein subunit"
                     /protein_id="HPB45193.1"
                     /translation="MGEPLTLDDLCDVGADIDGNAPTGADPAALWAERKLSYRLVGPL
                     NRRRFRVVVVGTGLAGGAAAAALGELGYQVECLTYHDAPRRAHSVAAQGGINAARSRK
                     VDNDSVARMVIDTVKGGDFRGREADCYRLAEESLRVIDHLDAIGAPFAREYGGALRTR
                     SFGGVQVSRTYYTRGQTGQQLQVAVTQALLRQVAAGTVKLSTRHEMLDLIVEDGRAQG
                     VVARDLVTGELKAITGHAVILCTGGYGNVFFQSTLAKSSNATAAWRAHRRGAYFASPS
                     FVQFHPTALPVNAPWQAKTTLMSESLRNDGRVWVPLRAGDDRPANEIPESERDYYLER
                     KYPSYGNLAPRDISSRSAREQIDAGHGVGPLRNSVYLDFRDSLERLGRDVIAERYGNL
                     FQLYREATGEDPFVVPMRIAPGAHFTMGGLWCDYDQRTSVPGLFVGGEAANNYHGANR
                     LGANSLLSASVDGWFTLPYSVPDYLAGLLGTAPLDPAGPSASAAIAEVEAGIERLCRI
                     GGTRPASVFHRQLGELLYRHCGVTRSAESLVVGIEAIRALRAEFWRDLAVVGGAAEFN
                     QELERAGRVADYLELAELMCIDALDRDESCGAHFRTEHQSADGEAVRNDEDYAFVSAW
                     EYADRPIRHREPLRFEHVGVQTRSYA"
     gene            4388..>4778
                     /locus_tag="PLP95_05000"
     CDS             4388..>4778
                     /locus_tag="PLP95_05000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020378469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HPB45194.1"
                     /translation="MRLNLRVWRQHDAHSPGEFTVYEVDDASPDMSLLELLDHLNERL
                     VTRGTAPIAFDSDCREGICGACGITVDGRAHGPVPNVASCHQHLRSFTDGATVTLEPF
                     RTGAFPVVRDLAVDRSALDRLIEAGGFV"
BASE COUNT          751 a         1579 c         1574 g          874 t
ORIGIN      
        1 gggccgctgt caatcaacga ggtcggtgcg caggtgtacc tgtccggtca cacggtgaag
       61 tggtacatca gccgcatcta tcgaaagctg cacgttcaca gtcggagcga ggcggtcgaa
      121 cgcgcgagga ccctcgggct tttcgacacc gcgcgttagg acccctgcga cgtccggaac
      181 gggacggtct ggaggatccg gaaaaccacc cgggtggaat cgaagaccac ccgcgagcgt
      241 ccacaggggg tcgccggtcg gtggtagtcc attccacacc gctccctgag gagcgtttag
      301 gtcgcttgga aagggtcgcg atgacagttg acagcgcttt ggatggaggc ttgggacccc
      361 tcgacatcct cataatcggc tttccggacg gcaaacccga tccggtcggt ttcgatcggt
      421 tggctgccct tgtcgacgcc ggaacgattc ggatcctcga cctcgagttc gttctacgtg
      481 acgagggagt ggcctacctc gccaagtcga ccgatctacc gccggtcgac ggcttcgatg
      541 ccgacttctg ggcgggggcg agttcccacc tgctcgaccg cgacgatctc gatcatctcg
      601 ccgccgactt ggaagaggga gaactggggc tcgtcgtgct gatcgagcag agctggatgc
      661 tcggattgat cgatgaatgg gttggtaacg gcgcgcatct catcgcggat gacggccttc
      721 cgtcccggga tctcgtcgac gcactcgacg ccgcggaacg acgcgaaggg agcgactgat
      781 ggggctgatc aaaaccgcca cgcgggttgc ggtcgccagc agcgttcacg gacgggttca
      841 acgccgccag caggagcgat gggcggccca gccccctcag ccgccagctg ctccaccgat
      901 gcccgcccct catgtggcac ctgcgccgcc ggcacctgca ccgccggcgg tcgatctgca
      961 acagcaggtc gatttgttgc gccagctcgg cgaacttcgc gacgcgggca tcatcgacga
     1021 ggccgagttt gctgcgaaga aagccaagat cctcggctga caacagcggt atcgttcggc
     1081 gaaccgcccg acgtatgaaa gaggcccgcc atggtccgac tccttgcgac tgctgtcgtc
     1141 gccttcttat ccaacgccgt cggtctgatc gtggcggcgc tgattcttga cgatgtccgt
     1201 ctcgggacgt tgggattcgt catcgctgcg ctcatcttca ccggcgtgtc ggtgctgacc
     1261 gaaccgctcc tgcgtcaggc cgcgatgaag cgagctcctg cgttgctcgg cagcaccgcg
     1321 ctccttacgg tgctcgtcag cctcatcgtc actgcgatca tcagcgacag cctctccatc
     1381 aacggtctcg tcacctgggt gcttgccacc atcgtcgtgt ggctcatctc gtttgtagga
     1441 cggctcgtgt tccccctgtt ggtgttcaag aagacgctgt cggcagcgag gtcgtcgcgc
     1501 tcggcatgat gcagcggttg gcgctcaccg cgtcgctgat ctcggccggc gtcgcgtgaa
     1561 gcgtcgttgc ctcatcggcg gttgccacca ggtcctcggt tgaggtcgat cggcttgccc
     1621 ggtcgggtgc gctgagcgcg atcgggccca aagaccctgc atcgcaacgc accctcgggt
     1681 catcatgggt tccatgatga cccgccaccc aacgggggtc gcccgtcggt cggcgacaac
     1741 tcccagcgtg acgagtgcca gcttgaaggt cgccatggcg gtgtcgggat ccgtgctcat
     1801 ggccttcgtg ttcgtgcaca tggtgggcaa cctcaaggtc tttctgggtc gcgacgaact
     1861 caaccactac gccgccttcc tgcgcgagtt cggggtgccg ctcatgccgc acgggtggtt
     1921 cctctggatc gtgcgagtgc tgctcacggt ctgcgtggtc acccacatgg ccagcgctgc
     1981 ggtgctcacc tcgcgggcgc gcgccgccgg ccgcaaccgg acgactcgtg gccatcaggt
     2041 gagctggtgg cggtcgttca cctcccgaac gatggccgtg agcggggtgg tcatcaccgc
     2101 gttcgtcggg ttccatcttc tcgatctcac gttgggggtg gccgcggccg acggatttcg
     2161 ccaccccgcc accgtcgatg gcgccaccgc ctacttcgcc tacgagaacc tcgtcggaag
     2221 cttccaacgg ccagcggccg cggcgctgta cgtcttggcc atgttggtgc tgggagcgca
     2281 tctttcgcac gggtcgtggt cggtcatcca cgatcttggc gtgaccggtg cccgggctcg
     2341 gcgaactgcg ttgatcgtgc tcagcgttgt ggccgctgcg gtggtggtcg gcaacgtctc
     2401 gatcccgctt gcggtccagg cggggtgggt gagctgacat gggcgagccg ctgacgctgg
     2461 acgacttgtg tgatgtcggc gccgacatcg acggcaacgc tcctaccggg gcagatcccg
     2521 ctgcgctgtg ggccgaacgc aaactctcgt accgtctcgt cggcccgctc aaccgtcgac
     2581 gcttccgagt ggtggtcgtc ggcaccggcc tcgcgggcgg cgccgcggca gccgcgcttg
     2641 gcgagctcgg ctatcaggtg gagtgtctca cctatcacga cgctcctcgg cgggcgcact
     2701 cggtcgcggc gcagggtggc atcaacgctg cacgttcacg caaggtcgac aacgactcgg
     2761 tcgcccgaat ggtgatcgat acggtcaaag gcggcgactt tcgtggacgc gaagccgact
     2821 gctaccggct cgccgaggaa tcgttgcggg tgatcgacca tctcgatgcc atcggggccc
     2881 cgttcgcccg cgagtacggc ggggcgcttc gaacgcgctc gttcggggga gtgcaggtca
     2941 gccgcaccta ttacacgcgc ggccagaccg gccaacagct gcaggtcgcc gtaacccagg
     3001 cgctgctgcg ccaggtcgcg gccgggacgg tgaagctgtc gacccgccac gagatgctcg
     3061 acctgatcgt ggaggacggc cgtgcgcagg gcgtcgtcgc ccgcgacctc gtgacgggtg
     3121 aactcaaggc gatcacggga cacgcggtga ttctgtgcac cggcggctac ggcaacgtgt
     3181 tcttccagtc gacgctcgca aagagttcca acgccaccgc ggcatggcgg gcacaccggc
     3241 gcggcgccta cttcgcgtcg ccgagtttcg ttcagttcca ccccacggcg ctgcccgtga
     3301 acgccccgtg gcaggcgaag accactctga tgagcgagtc gctacgaaac gacggtcgcg
     3361 tgtgggttcc gcttcgggcc ggcgacgacc ggccggccaa cgagattccc gagtccgaac
     3421 gcgactacta cctcgaacgt aaatacccga gctacggaaa cctcgcgccg cgcgatatct
     3481 cgtcgcggtc ggcacgagag cagatcgatg ccggacatgg cgtcggcccg ctgcgcaaca
     3541 gcgtgtatct cgactttcgc gactcgctcg aacgcctcgg tcgcgacgtc atcgcggagc
     3601 gttacggcaa tctgtttcag ctctatcgcg aggcgaccgg ggaggacccg tttgtcgtac
     3661 cgatgcggat cgcacccggt gctcacttca cgatgggagg gttgtggtgc gactacgacc
     3721 aacgcacctc ggtgccgggg ctgttcgtcg gaggagaagc agcgaacaac taccacggcg
     3781 cgaatcggct cggcgccaac tcgctgctgt ccgcctcggt ggacggctgg ttcacccttc
     3841 cgtactcggt tccggactac ctcgccgggc tcctcggcac cgcgccgctg gatcccgccg
     3901 gcccgagtgc gagcgccgcg atcgccgagg tcgaagccgg tatcgagcgg ctctgccgta
     3961 tcggcggcac ccggccggca tcggtgtttc accgtcaact cggcgaactt ctctaccggc
     4021 actgcggcgt cacccgaagc gcggagtcgc tcgtcgtcgg catcgaagcc attcgtgccc
     4081 ttcgggcgga gttctggcga gacctcgcgg tcgtcggcgg tgcggcggag ttcaatcagg
     4141 aactcgaacg cgccggcaga gtcgccgatt acctcgagct cgccgagctg atgtgcatcg
     4201 acgcgctcga tcgcgacgag tcctgtggcg cgcatttccg caccgaacac cagagcgccg
     4261 atggtgaagc cgttcgaaac gacgaggact atgcgttcgt gtcggcctgg gagtacgcgg
     4321 atcggccgat tcggcaccgc gaaccccttc ggttcgaaca cgtcggtgtg cagacgagga
     4381 gttacgcgtg agactgaacc tgcgcgtctg gcgtcagcac gacgcacaca gccccggcga
     4441 gttcaccgtc tacgaggtcg acgatgcgtc gcccgacatg agcctgctcg aactcctcga
     4501 ccacctcaac gaacgtctcg ttacccgggg gacggccccg atcgccttcg attccgactg
     4561 ccgtgagggt atctgcgggg cgtgtgggat cacggtggat ggccgcgccc acggcccggt
     4621 cccgaacgtg gcgagttgcc accagcatct gcgcagcttc accgacggtg cgaccgtgac
     4681 cctcgaaccg ttccgcaccg gggcctttcc tgtcgtgcgc gacctcgccg tcgaccgatc
     4741 ggcgctggac cggctcatcg aagcgggcgg cttcgtga
//
LOCUS       DAOOMA010000136         4762 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_21580_length_4762_cov_3.751009, whole genome shotgun sequence.
ACCESSION   DAOOMA010000136 DAOOMA010000000
VERSION     DAOOMA010000136.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 4762)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4762)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4762
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21580_length_4762_cov_3.751009"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..213)
                     /locus_tag="PLP95_05005"
     CDS             complement(<1..213)
                     /locus_tag="PLP95_05005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB45195.1"
                     /translation="MTPVWPVVSARSATAAAPPSPHARHDRAGGSTVHTALNRTDGRS
                     TMKLHLDAATTAIVTMECQRGVIGDLS"
     gene            212..850
                     /locus_tag="PLP95_05010"
     CDS             212..850
                     /locus_tag="PLP95_05010"
                     /inference="COORDINATES: protein motif:HMM:NF014591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphatase"
                     /protein_id="HPB45196.1"
                     /translation="MADRLVLASTSRYRAEVLASAGYSFDVEAPEIDERAFDDRFESW
                     GPQRYVMELATAKAEAVARRLVGDGPELSQRHLVLGCDQIAVLGGDDRPTLLHKPGSA
                     ERAVAQLMEMSGRTHRLINAFVLVDASDGERWSEIDVHHVTMRSYTVGEACEYVDRFE
                     PLDCAGSYRIEDDADLLDRVEGEHRSGVIGLSIPSVARLLDAAGSGSSIPRS"
     gene            930..1382
                     /locus_tag="PLP95_05015"
     CDS             930..1382
                     /locus_tag="PLP95_05015"
                     /inference="COORDINATES: protein motif:HMM:NF014062.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HPB45197.1"
                     /translation="MDDPETMRWVWLIATAVFLGGELLTAGALVLLPFALGTGVAATI
                     AFAGVDVEWQWLMFLLVAIVTSFAFLPLRRRLDRAPQHVGVGAGRLLNQEAVVLETIE
                     EGPTGVGMVRVGRETWRATSGDGRRFDIGTHVTVTYVEGTGVVVAERT"
     gene            1472..2482
                     /locus_tag="PLP95_05020"
     CDS             1472..2482
                     /locus_tag="PLP95_05020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012228736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HPB45198.1"
                     /translation="MVTAVVILVLAGLLLVFLVSTIKIVRPYQRGVVERLGRYHGTVE
                     PGMRIIYPFIDKLRYVDMREQVIDVPPQEVITADNVVVSVDAVVYYEPTDPQRLIYNV
                     ANFILAVTKLAQTNLRNLIGDLQLDQALTSRDSINMSLRSILDDATDTWGVRVKRVEI
                     QRIDPPVDVMSAMHEQMKAERTRRATVTTAEGERQAQITRAEGERQAAILRAEAVREQ
                     QRLEAEGQATAIKELADAERYKQIAEADGEADAIRAIYNAIHEGNPTNDLLAIRYLDT
                     LAKMSNGRATKIFLPADLSSGLSLLGSLGEMFRDSNLNPSGASDPGGPRQIPQGSVPP
                     QH"
     gene            2559..4067
                     /gene="guaB"
                     /locus_tag="PLP95_05025"
     CDS             2559..4067
                     /gene="guaB"
                     /locus_tag="PLP95_05025"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012225895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HPB45199.1"
                     /translation="MAPVSDDDLGGDAAFSARFGRIGLTFDDVLLLPAHSDVLPHEAD
                     TSTMFTPDIELALPIVSAAMDTVTEARMAIALARLGGIGVLHRNLSPEEQAAHVDRVK
                     RSESGTITDPVTLQPHLPVQAALDLMARFSISGVPITNEDNKLVGILTNRDLRFETNY
                     ARPISEVMTSENLHTAPVGTTLDEAQEMFRHIKVEKLPVVDADGTLRGLITIKDLQKR
                     VEFPNATKDSEGRLRVAAAVGTGADVFERAKALIDAGVDALVIDTAHGHATSVADTVR
                     EMRRRWDGPLVAGNVATAAAAEALIDAGADAIKVGIGPGSICTTRVVAGIGVPQISAV
                     FDCARVASARGRTVIADGGIQFSGDVAKALAAGADTCMFGSLLAGTDESPGETVLYQG
                     ERYKIYRGMGSLGAMKARSFSKDRYFQEHVVDAEKLVPEGIEGRVAYVGTVSGIVHQL
                     VGGLQQAMGYVGAPTIAELHNAEFIRMTSAGLREAHPHDITITEQAPNYRKF"
     gene            4200..4652
                     /locus_tag="PLP95_05030"
     CDS             4200..4652
                     /locus_tag="PLP95_05030"
                     /inference="COORDINATES: protein motif:HMM:NF013234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannose isomerase type II C-terminal
                     cupin domain"
                     /protein_id="HPB45200.1"
                     /translation="MSESMRIDEATPEVGFPKLVVNSMNGEVRSNHSNSPMYEDRPWG
                     SYTVLSDEPDHKVKSITVAPGQRLSYQRHSHRSEHWFVITGDATVTLDGVVFQVGPGH
                     YADVPQGVAHRLENQGDEPLVIIEVQYGDYFGEDDIIRLSDDYGRTDD"
BASE COUNT          821 a         1453 c         1601 g          887 t
ORIGIN      
        1 cgacaggtcg ccgatgaccc cgcgttggca ttccatggtg acgatcgcgg tggttgcggc
       61 atcgaggtga agcttcatcg ttgaccttcc gtcggttcgg ttgagggcgg tgtgcacggt
      121 actgccgccg gcgcggtcgt gccgagcgtg tggcgatggc ggtgccgccg ccgtggcgct
      181 gcgggctgac acgaccggcc agactggggt catggccgat cgcttggtgc tggcgtcgac
      241 gagtcgctat cgagccgagg tgttggcctc ggcgggatac tcctttgacg tcgaagcccc
      301 cgaaatcgac gagcgggcct tcgacgaccg gttcgagtcg tggggcccgc agcgctacgt
      361 gatggagttg gccacggcga aggccgaggc ggtcgcccga cgtctggtgg gggatggacc
      421 ggagctgtca caacgccatc tcgtgttggg gtgcgaccag atcgcggtgc tcggcggcga
      481 cgaccgaccc acgctgttgc acaagccggg gtcggccgag cgggccgtgg cccaactgat
      541 ggagatgtcg ggccgaaccc accggctgat caacgcgttc gtcctggtcg acgcctcaga
      601 cggtgagcgt tggagcgaga tcgatgttca tcacgtgacc atgcgctcgt acaccgtggg
      661 ggaggcctgc gagtacgtcg atcgtttcga gccgctcgat tgcgccggct cgtaccggat
      721 cgaagacgat gccgacctgt tggatcgggt cgagggggaa caccgaagcg gcgtcatcgg
      781 gctgtcgatt ccgagcgtcg ctcgactgct cgacgccgcc ggaagcggtt ccagcatccc
      841 gcgatcctga cgtcgcaccc gaactccagc cgaagcggcc gagccgaaac tccaagccga
      901 aacagccgat gttccggtag cctctcggca tggacgatcc ggagacgatg cgctgggtgt
      961 ggctgatcgc cacggcggtg ttcctcggcg gcgagctgtt gacggccgga gcgctggtgt
     1021 tgttgccatt cgcgttgggc accggggtgg cggcgaccat cgcgttcgcc ggcgtcgacg
     1081 tcgagtggca gtggttgatg tttctgctgg tcgccatcgt gacgtcattc gcgttccttc
     1141 cccttcgtcg gcggctggac cgggcaccac aacacgtcgg ggttggcgcc ggtcggttgc
     1201 tgaaccagga agcggtcgta ctcgaaacga tcgaagaggg tccgaccggg gtcggcatgg
     1261 tgcgggtcgg gcgagagacg tggcgggcga cgagtggcga cggtcgtcgt ttcgacatcg
     1321 gcacccacgt caccgtcact tacgtcgagg gcaccggtgt ggtcgtcgcc gaacgcactt
     1381 gagcccgttg cccgctcgta gcccggtcca tcacatccgt ccgtagccaa cccgttcgtc
     1441 accacatcca ccaccaaccg aaagggcctc gatggttacc gctgtcgtca tcctcgtact
     1501 cgccggactg ctgctggtct ttctcgtctc gacgatcaag atcgttcggc cataccaacg
     1561 cggcgtggtc gagcggctcg gtcgatacca cggcaccgtc gaacccggca tgcggatcat
     1621 ctatccgttc atcgacaagt tgcgctacgt cgatatgcgt gagcaggtca tcgacgttcc
     1681 gccacaggag gtgatcaccg ctgacaacgt ggtggtgagt gtggacgcgg tggtctacta
     1741 cgaacccacc gaccctcagc ggctcatcta caacgtcgcc aatttcatcc tggcggtgac
     1801 gaagctggcg cagacgaacc ttcgaaacct gatcggtgac ctgcaactcg accaggcgct
     1861 cacctcgcgt gattcgatca acatgagcct gcgatcgatc ctcgacgacg ccaccgacac
     1921 ctggggtgtg cgggtcaagc gcgtggaaat ccagcgcatc gatccgccgg tcgacgtgat
     1981 gagcgcgatg cacgagcaga tgaaggccga acgtacgcgt cgtgccaccg tcaccaccgc
     2041 cgagggtgag cgtcaggccc agatcacccg ggcggagggt gagcgtcagg ccgcgatttt
     2101 gcgggccgag gccgtgcgtg aacagcagcg gctcgaagcc gagggtcagg cgacggcgat
     2161 caaggaactc gcggatgccg agcggtacaa gcagatcgcc gaggccgacg gcgaggccga
     2221 cgcgattcgg gcgatctaca acgcgatcca tgaggggaac cccaccaacg atctgttggc
     2281 gattcgctac ctcgacacgc tcgcgaagat gtcgaacggt cgggcgacca agatcttcct
     2341 gccggccgat ctcagttcgg ggctgagcct gttgggcagt ctcggcgaga tgtttcggga
     2401 ttcgaacctg aacccgtccg gtgcgtccga ccccggtggt ccacggcaga ttccccaggg
     2461 ttcggtcccg cctcagcact gacccgacgg cggtggctcc gccagcaccg cggaaatcct
     2521 gtgtgaacgg tgcccctcgc tgaggggtat cgttacgtat ggcacctgtt tccgacgacg
     2581 atctcggcgg cgacgccgca ttctctgcac gattcggccg gatcggcctg accttcgacg
     2641 acgtgttgtt gttgccggcg cacagtgacg tgttgcccca cgaggccgac acctcgacga
     2701 tgttcacccc cgatatcgag ttggcgctgc cgatcgtcag cgccgcgatg gacaccgtca
     2761 ccgaggcgcg catggcgatt gccttggccc gtctcggtgg catcggggtg ttgcaccgca
     2821 atctttcgcc cgaggaacag gccgcccatg tcgatcgggt gaagcgttcc gagtcgggga
     2881 cgatcaccga cccggtgacg ctgcagccac atctaccggt tcaggccgcc ctcgatctga
     2941 tggcacgttt ttcgatttcg ggtgtgccga tcacgaacga ggacaacaag ctcgttggaa
     3001 tcctgaccaa cagagacctg cgattcgaaa cgaactatgc gcggccgatc tccgaggtga
     3061 tgacctcgga gaacctgcac accgctccgg tcggcacgac gctcgatgaa gctcaggaaa
     3121 tgtttcgtca catcaaggtc gaaaagctgc cggtggtcga cgccgacggc acgttgcgcg
     3181 ggttgatcac gatcaaagac ctccaaaagc gcgtcgagtt cccgaacgcc accaaggact
     3241 ccgaggggcg cttgcgggtt gcggccgcgg tcggtaccgg cgcggatgtg ttcgaacggg
     3301 ccaaggcgct catcgacgcc ggtgtcgacg cgctcgtcat cgacactgcg cacggccatg
     3361 cgacctcggt tgcggacacg gtgcgcgaga tgcgccgccg ctgggatggt ccgctcgtgg
     3421 ccggcaacgt cgcaacggcg gctgcagccg aggcgctcat cgatgcgggg gccgacgcca
     3481 tcaaggtcgg gatcggtccg ggctcgatct gcacgacccg ggtggtcgcc ggcatcggtg
     3541 tgcctcagat cagtgcggtg ttcgactgtg cccgggtggc gagcgctcgc ggtcgtacgg
     3601 tgatcgcgga tggcggtatt cagttctccg gcgacgtggc caaggcgctg gcggcggggg
     3661 ccgacacctg catgttcggg tcgctgctcg ccggcaccga cgaaagcccc ggcgaaacgg
     3721 tgttgtacca gggggagcgc tacaagatct atcggggtat gggcagtctc ggggcgatga
     3781 aggcgcggtc gttttcgaag gaccggtatt tccaggagca cgtcgtcgac gcggaaaagc
     3841 tggtgccgga ggggatcgaa ggccgtgttg cgtatgtcgg gacggtgtcg gggatcgttc
     3901 atcaactcgt cggtggtctg cagcaggcca tgggttatgt gggggcgccg acgatcgccg
     3961 aactgcacaa cgccgagttc atccgcatga cctccgcggg gcttcgcgag gcgcatcccc
     4021 acgacatcac gatcaccgaa caggcgccca actaccgcaa gttctgatcg gccgagttcg
     4081 cattggtgcc acctgattcc cgtacacttg tgcgctaagt gcctgcggtc ggcaggatca
     4141 cgttgaatcg cttgcgattc ggtgctgtgc gatgaccggt cgcagatgga ggtgtaacgg
     4201 tgagtgaatc gatgcgaatc gacgaggcaa ctccggaagt tgggtttccg aagctggtgg
     4261 tcaactcgat gaacggcgag gtccgatcca accactccaa ctcgccgatg tacgaggatc
     4321 gcccgtgggg ttcctacacg gtgttgtccg acgagcccga ccacaaggtg aagtcgatca
     4381 ccgtcgctcc cggccagcgg ctgagttatc aacgtcattc gcatcgttcg gagcactggt
     4441 tcgtcatcac tggcgacgcg accgtgacgt tggacggtgt ggtgtttcag gtggggccgg
     4501 gtcactatgc cgatgtgccc caaggcgttg cgcatcgcct cgagaaccag ggcgatgaac
     4561 cgctcgtcat catcgaggtg cagtatggcg actacttcgg tgaggacgac atcatccgtt
     4621 tgtccgatga ttacgggcgt accgacgact gagcgctgct ctacactcga tctgtgaccg
     4681 catttcacgc cctttcgctg ttgttggcgc atccggaact cgatgatccg aactttcgtc
     4741 gcagcgtcgt gtatctgttg ga
//
LOCUS       DAOOMA010000137         4760 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_21593_length_4760_cov_3.669288, whole genome shotgun sequence.
ACCESSION   DAOOMA010000137 DAOOMA010000000
VERSION     DAOOMA010000137.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 4760)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4760)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4760
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21593_length_4760_cov_3.669288"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(24..1616)
                     /locus_tag="PLP95_05035"
     CDS             complement(24..1616)
                     /locus_tag="PLP95_05035"
                     /inference="COORDINATES: protein motif:HMM:TIGR00350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HPB45201.1"
                     /translation="MGNRRRRRGRRSWGQRLLIAFNSLLALACLAAAGAFHVARAKAA
                     EVPVYHIDSIANARISDTEVRNILMVGTDDSSGLDDDDPTKKGRGSEHLADTIMILRV
                     DPVAKTAAILSIPRDTWVPVAPNWSSTKINGALNRTEGPKQLIATIRHSFGISIDNYV
                     EVDLGGFKEVYRALGGIRVYNKYPIKDPKTSLWLPETGCITLDPEQALAYARTRDLWW
                     QDSDTYTKGGKWKHDTTVDYGRIARQQDLIKQTAQSAIERGIRNPITAYKLVNAAIGS
                     VTTDDTINADWVVDLIQTFREFPVDQLATHRLPTSSTNKGGASAESVQWDEAADLLRF
                     FRGVPAEGEVRASDVIVSVPDSFEAADGLVSALDGAGFNATAESAKAFNGSTTAKKDT
                     IRYGADGAEAAAVLAAHLDLDPAREIEFVLDEDLPGRRLEFIPRLNSEPSLRPEPLPT
                     DMVRQAAEIVVPTTTTTTTTMRTSQAGNHPSTTDASATTTSVAETVVGNTTTTSSLDQ
                     DESEPATVGMIPFDAAMAASCR"
     gene            1717..2757
                     /locus_tag="PLP95_05040"
     CDS             1717..2757
                     /locus_tag="PLP95_05040"
                     /inference="COORDINATES: protein motif:HMM:NF012640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate-binding protein"
                     /protein_id="HPB45202.1"
                     /translation="MAKRFDPAPVAVVGDRLCSELADVTSDFTALDSSGFWAVVIPYD
                     GEAVCARFATVRPAVPWAGAPWRGPALDSWHSSLDRDGFEAGVRSIRESIAAGDVYQV
                     NLTRRLRARLTDPEHQRIEALGAALALGNPAPFSAVVSLPDHDIAVASASPELFLARD
                     GDVVRSSPIKGTAPTPEEFLDKDRAENVMIVDLVRNDLGMVCDWGTVTVPSLLATEEH
                     PGLFHLVSTVEGRLRSGLGWADAIGATFPPGSVTGAPKISACSIIDRLEPDSRGVYCG
                     AIGWVDADSRRGALNVAIRTFWIEGDELNFGTGGGITWGSDPTGEWNETELKARRLLG
                     VASATTSRGDTT"
     gene            2754..3590
                     /locus_tag="PLP95_05045"
     CDS             2754..3590
                     /locus_tag="PLP95_05045"
                     /inference="COORDINATES: protein motif:HMM:NF013247.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HPB45203.1"
                     /translation="MSNPTNPSVWIDGHLVSADAARVPYDDHGITVGDGVFETVKLTE
                     SGPFALSRHLERLRRSADAMKIPAPSDEVVRAAIAEVVATHEGDGFLRVTLTAGPGPL
                     GSNRDELTPRLIVAVRPGEVRVAPTTVVLVGYTRNERGALAGVKSTSYGENVVALAAA
                     AESGASEALFANTVGDLCEGTGSNVFVGFGDRLVTPPLHSGCLDGITRALLLEAGVGT
                     EQDIPVNSLGDATEMFLVSTAREVQPVTAMGDLVLPLAPGPLTTRAREVWLERVAERL
                     DP"
     gene            complement(3604..4404)
                     /locus_tag="PLP95_05050"
     CDS             complement(3604..4404)
                     /locus_tag="PLP95_05050"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HPB45204.1"
                     /translation="MDLTRATSALAGTRFSRITWVGETGSTNADLLDAARRGEGEQVL
                     GAEFQTAGRGRLGRDWSAGAGASILCSVLVRPAPETSSPHLATAAVGLAAAEAIEHVV
                     GWRVGIKWPNDLVAAAASTGTHDDRKLSGILAEAVVVDGRVDAVVVGIGINVNWPELP
                     AELSDTATALNHLAGHDVDRTDVVIELIRRTAMHLDAAAGPDGRVGLRDAVSARSATL
                     GRRVRIEQPNGLLEGMAVALGPDGELVVETATGPVTVSVGDVVHLRPI"
     gene            4421..>4760
                     /locus_tag="PLP95_05055"
     CDS             4421..>4760
                     /locus_tag="PLP95_05055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020378269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin carboxylase N-terminal domain-containing
                     protein"
                     /protein_id="HPB45205.1"
                     /translation="MFSKVLIANRGEIAVRVIRACKELGISTVAVYSDLDRDALHVRL
                     ADEAYALGGQTAAESYINTDKLLEVIKQSGAEAVHPGYGFFSENSDFAKAITEMGVKF
                     IGPRPEAIDVM"
BASE COUNT          760 a         1639 c         1532 g          829 t
ORIGIN      
        1 ctcccacaac aacccggtcg ggtttaccga catgacgctg ccatcgcagc atcgaacggg
       61 atcataccga ccgttgccgg ttccgattca tcttggtcaa ggctagaggt ggtcgtcgta
      121 tttccgacca ccgtctccgc caccgacgtt gtagttgccg acgcgtcggt cgtcgatggg
      181 tgattacccg cctggctggt cctcatggtg gtcgtcgtgg tcgtcgtggt cggcacgacg
      241 atctccgccg cttgacgaac catgtcggtc ggcagcggct cgggacgaag cgacggttcg
      301 ctgttgagtc tcgggatgaa ctcgagacgc cgcccaggaa ggtcctcgtc caacacgaac
      361 tcgatctcgc gggccgggtc caggtcgaga tgggccgcca acaccgctgc cgcttccgct
      421 ccgtcggccc cgtagcgaat cgtgtccttc ttggcggtgg tcgacccgtt gaaggccttg
      481 gcgctctccg ccgtcgcgtt gaaacccgct ccgtcgagcg ctgagaccaa gccgtcggcg
      541 gcttcgaagg aatcgggcac cgacacgatc acatcgctag ccctcacctc accctcggcc
      601 ggcacgccgc ggaagaagcg caacaggtcg gccgcttcat cccactgcac gctctcggcc
      661 gacgctccgc ccttgttcgt tgaactcgtc ggcaaacgat gcgttgcgag ctggtcgacc
      721 gggaactccc ggaaggtctg gatgagatcg acgacccagt ctgcgttgat cgtgtcatcg
      781 gtggtcaccg acccgatcgc cgcattcacg agcttgtacg cggtgatcgg gtttcgaatc
      841 ccacgctcga tggccgactg ggccgtctgc ttgatcaggt cctgctgtcg ggcgatccgg
      901 ccgtaatcca ccgtggtgtc gtgcttccac ttcccgcctt tggtgtaggt gtcgctgtcc
      961 tgccaccaca gatcgcgggt tcgggcgtac gccaaggcct gttcggggtc gagggtgatg
     1021 cagccggtct cgggcagcca caggctcgtc ttggggtcct tgatcgggta cttgttgtag
     1081 acgcgaatgc caccaagtgc ccggtacacc tccttgaaac cgccgaggtc gacttcgacg
     1141 tagttgtcga tcgagatccc gaagctgtgg cggatcgtgg cgatcagttg cttcgggcct
     1201 tcggtgcggt tgagggcacc gttgatcttg gtgctcgacc agttcggagc gaccggaacc
     1261 caggtgtctc gcgggatcga caggatcgcg gcggtcttgg ccaccgggtc gacccgcagg
     1321 atcatgatcg tgtcggcgag atgctcggaa ccacgaccct tcttcgtggg gtcgtcgtca
     1381 tcgagccccg acgaatcgtc ggtgcccacc atcaagatgt tgcgcacttc ggtatcggag
     1441 atccgggcgt tcgcgatcga gtcgatgtga tacacgggga cttcggcagc tttggcccgc
     1501 gccacatgaa atgctccggc cgctgccagg caggcgagtg ccagcagcga gttgaacgcg
     1561 atgaggaggc gttgacccca actgcgacgt ccgcgacgtc gacggcggtt acccatggtg
     1621 ggcgatccta cccgtggcta tggcgtcacc tgaggctggc acctgagaac gccgcgcacc
     1681 gactgacgct tccacggctg ccctagtgtc ggggcggtgg ccaagcgttt cgaccccgca
     1741 cccgtcgccg ttgtcggcga ccgtttgtgc tccgaactcg ccgatgtcac ctccgacttc
     1801 accgcactcg attccagcgg cttttgggca gtggtcatcc cctacgacgg cgaggcggtc
     1861 tgcgcacggt tcgcgacggt gcgccccgct gtgccctggg ccggggcacc gtggcgcgga
     1921 ccggcgctcg actcatggca cagcagcctc gaccgcgacg ggttcgaggc cggagtccgc
     1981 tccatccgcg agtccatcgc tgcgggcgac gtctaccagg tgaacctcac ccgccgactt
     2041 cgtgcccgcc tcaccgaccc tgagcaccaa cgcatcgagg cactcggcgc cgcgctggcg
     2101 ctgggaaacc cggccccatt cagcgcggtc gtgtcgctgc ccgaccacga cattgcggtg
     2161 gcgtcggcct ctcccgagct ctttctcgcc cgcgacggcg acgtcgtgcg ctcgtcgccg
     2221 atcaaaggta cggcgccgac cccggaggag ttcctcgaca aggaccgcgc cgagaacgtc
     2281 atgatcgtcg acctcgtgcg caacgacctc ggcatggtgt gcgactgggg aacggtgacg
     2341 gtgccgtccc tgctggcgac cgaagagcat ccggggctgt tccacctggt gtcgaccgtt
     2401 gaaggacgcc tccgttccgg cctcggatgg gccgatgcca tcggcgcgac gtttcccccg
     2461 ggatctgtca cgggtgcacc caagatcagc gcgtgttcga tcatcgatcg gctggaaccc
     2521 gactcgcgcg gcgtgtactg cggtgccatc ggctgggtcg atgcggactc ccgccgaggc
     2581 gcactgaacg tcgccatccg gaccttctgg atcgagggcg acgagttgaa cttcggcacc
     2641 ggcggcggaa tcacgtgggg atccgacccg accggcgaat ggaacgaaac cgaactcaag
     2701 gcgagacgac tcctcggggt cgcctcggcg acgacctcac gaggagacac cacatgagca
     2761 acccgaccaa cccgagcgtc tggatcgacg gccacctcgt gtcggccgac gccgcgcgcg
     2821 tgccctacga cgaccacggc atcaccgtcg gcgacggcgt tttcgagacc gtcaagctga
     2881 ccgaatccgg gccgttcgcg ttgtcccgcc accttgagcg actccgccgt tcggccgacg
     2941 ccatgaaaat tcccgccccg tccgatgagg tggttcgcgc cgcgatcgcc gaggtggtcg
     3001 ccacccacga gggcgacggg ttcctgcgcg tcacgctgac cgccggaccg ggaccgttgg
     3061 ggtcgaaccg cgacgagctc acgcctcggc tgatcgtcgc cgtgcggccc ggcgaagtcc
     3121 gcgtcgcccc gacgacggtg gtgctcgtgg gctacacccg caacgagcgc ggcgccctcg
     3181 ccggggtgaa gtccacctcg tatggggaaa acgtcgtggc gcttgcggcc gccgccgagt
     3241 cgggcgcctc ggaggcgctg ttcgccaaca cggtcggaga tctgtgcgag ggcaccggtt
     3301 cgaacgtgtt cgtcggcttc ggcgaccgac tcgtcacccc accgctgcac tcggggtgcc
     3361 tcgacggcat cacgcgggcg ctgctcctcg aagccggggt cggcaccgag caggacatcc
     3421 cggtcaattc cctcggcgac gcgaccgaga tgttcctggt atcgaccgca cgagaggtgc
     3481 agcccgtgac cgctatgggc gacctggtgc tccccctcgc gccgggcccc ttgacgacgc
     3541 gagcgcgcga ggtgtggctg gagcgcgtcg ccgagcgcct cgacccctga cggtcctgcc
     3601 gcctcagatg ggccgtaggt gcaccacgtc gcccaccgac acggtcaccg gacccgtcgc
     3661 ggtctcgaca acgagttccc cgtcggggcc gagtgcgacg gccatgcctt ccaacaatcc
     3721 gtttggttgc tcgattcgaa cacgccgacc caacgtcgcc gagcgggcgc tcaccgcatc
     3781 gcgcaaccca acccgtccgt cgggacctgc cgctgcgtcg aggtgcatgg cggtgcgtcg
     3841 gatcagctcg atcaccacgt cggttcgatc gacgtcatgg cccgcgaggt gattcagcgc
     3901 cgttgccgta tcactcaatt cggcgggcag ctcgggccag ttcacgttga tgccgatgcc
     3961 gacgacgacc gcatcgacac gcccgtccac gacgaccgcc tcggcaagta ttcccgacag
     4021 cttgcggtcg tcatgggtcc cggtcgatgc cgcagcggcc acgagatcat tgggccactt
     4081 gatcccgacc cgccagccga cgacgtgttc aatcgcctcg gcagccgcca acccaaccgc
     4141 ggccgtcgcc aggtggggac tcgaggtctc cggggcggga cgaacgagta ccgaacacag
     4201 aatcgacgca ccggcacccg cgctccagtc gcgacccagg cggccgcgac ccgccgtttg
     4261 gaattcggcg ccgagcacct gctcgccttc cccgcgccgt gccgcatcga ggaggtcggc
     4321 gttggtcgaa cccgtctcac cgacccaggt aatccgggaa aatcgggtcc cggcaagcgc
     4381 tgatgtggca cgcgtgaggt ccattcggcc acgatagagc gtgttttcca aagtcctgat
     4441 cgcaaaccgt ggtgagatcg ccgtccgagt cattcgggca tgcaaggaac tgggcatctc
     4501 caccgtcgcc gtctactccg acctcgaccg ggacgcgctg cacgtgcgac tcgccgatga
     4561 ggcctatgcc ctcggcgggc agaccgccgc cgagagctac atcaataccg acaagttgct
     4621 cgaggtcatc aagcaatccg gtgccgaggc cgtgcatccc ggctatggct tcttttccga
     4681 gaacagcgac ttcgccaagg ccatcaccga gatgggcgtg aagttcatcg ggccgcgccc
     4741 cgaggcgatc gacgtcatgg
//
LOCUS       DAOOMA010000138         4717 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_21839_length_4717_cov_5.790648, whole genome shotgun sequence.
ACCESSION   DAOOMA010000138 DAOOMA010000000
VERSION     DAOOMA010000138.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 4717)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4717)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4717
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21839_length_4717_cov_5.790648"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1085)
                     /locus_tag="PLP95_05060"
     CDS             complement(<1..1085)
                     /locus_tag="PLP95_05060"
                     /inference="COORDINATES: protein motif:HMM:NF039907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HPB45206.1"
                     /translation="MSQGTQPACGNLSAMTPDDLFQVADSVLDAARGGEQIEVVVSHA
                     RETEVRAYDGDVEAFTSSESRGVGIRVVHDGRQGFAYAGSIEADVVFETLAEARDNAM
                     FSTGDEFVGLVSPDGVEVVEMDLYDPRVAAMSPEEKIALAIELEQRVLEGDPRILGVE
                     MAEYSDSIDTAVLASTTGIRSASSETANALIVYSLAGDDDEVTTGFGFSVGRGPDALD
                     AEAAAHQAVDRALRLLGASRVASQRLTVVFDPWVTAQFLGVIGESFSGIEVFRGRSFL
                     GGRIGERIASPMVTLVDDPTDRRWPTASPIDDEGLATRRNVLIDGGVLNGFVHDGYSA
                     RANGVASTGSAVRAGFKSTPAAGVQALS"
     gene            1084..2073
                     /locus_tag="PLP95_05065"
     CDS             1084..2073
                     /locus_tag="PLP95_05065"
                     /inference="COORDINATES: protein motif:HMM:NF012517.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HPB45207.1"
                     /translation="MLFGLRFDLRNPAFANTTMTERYEAALEMAQWADERGAAFICVS
                     EHHSSPDGYLPSALTMAAAMIARTRTTRIWVNAIAAPLHHPIHLAEQCAVLDNLSGGR
                     FDLTVGAGYVRGEFEMFGVAMNRRPSLMTETVTALRQAWSGEPFEFRGRTVRVTPTPE
                     SKRGPGLTMGGSSEAAARRAARLGVGFIPSEPALWEFYRDELLQLGLRDPGPFVSGAD
                     TSVVILADDVSAGWRELAPYFLHETNAYGQWREPDDVATSYRPVETVEDLQDLGQYRV
                     LTPDQWRDELEAAGPFSLALLHPMVGGIPPKRGWEHLRMFERDVLDFDAAKPE"
     gene            2226..3074
                     /locus_tag="PLP95_05070"
     CDS             2226..3074
                     /locus_tag="PLP95_05070"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HPB45208.1"
                     /translation="MGLPIGGFRTIFSARTDANVLSLTFDDGPDPEFTEHVLTVLDRY
                     DVGATFCMMGHNALEHRSLAQSVVSHGHEVANHSLTHLDQTRLSPRELHDEIALGHQA
                     VVEATGSPVRYFRPPRGRLVGRASRVLAELNSDALLWSISGSLRAPTTTAKLTKLVID
                     ALHPGAIVCFHDGIGRGTFAREQRFARELKARRNAEIEALPAIIEAVIDRGYSFATVT
                     ELLGFEATNPYESEITSLDEPQIVDGEDSVIDWDDGSDDVDSPLNAADPEPVAAEETA
                     LESGVG"
     gene            complement(3103..3657)
                     /locus_tag="PLP95_05075"
     CDS             complement(3103..3657)
                     /locus_tag="PLP95_05075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB45209.1"
                     /translation="MLIGVGALLTVVLIVAGALLTWGANFSNDYVRDELASQNVFFPD
                     ADALKAEGRDDLLEFAGLQVTTGSHAERYASYIQGHLDGMADGKTYSQIEDRAARAAV
                     QEAIENGEDEAVIDELQAEADRLTSLRDSLFKGETLRGLLLTSYAWATIGQIAAIAAW
                     VAFGAALLMAVLVIAGLAHIRKAE"
     gene            complement(3735..4574)
                     /locus_tag="PLP95_05080"
     CDS             complement(3735..4574)
                     /locus_tag="PLP95_05080"
                     /inference="COORDINATES: protein motif:HMM:NF033580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
                     /protein_id="HPB45210.1"
                     /translation="MRALDREVIDAVWKSFEPLLPEHVDNHPKGGHRPRTPDRIVFHL
                     ILVRLVCGCSWVDAEALCGWQVSDTTARARFKLWVCHDVFRRAFEHALDAYDRIIGLD
                     LSEVAVDGSLHKAPQGGDGTGPNPTDRGRSGWKWSIGTDRNGIPVGWATDGANRHDIP
                     LLAPTLDSIAGRHLLADIDTIHLDRGYDANTVRATLAGYGLDDAVIAKRRKPGDPPHT
                     KVGLGLRWPVERTNSWLVNYGQLRRNTDRKRAHREAQLALAIVFILAAKLIDWRNRWD
                     PPT"
BASE COUNT          826 a         1550 c         1500 g          841 t
ORIGIN      
        1 gacaacgcct gcaccccggc ggccggcgtc gacttgaacc cggcccgcac ggccgagccg
       61 gtcgacgcga ccccgttggc ccgcgccgag tacccgtcgt ggacgaagcc gttcaacacg
      121 ccgccgtcga tgagcacgtt tcgccgcgtc gccaacccct catcgtcgat cggcgacgcc
      181 gtcggccacc gacgatcggt tgggtcgtcg acgagggtga ccatcggcga ggcgatcctc
      241 tcgccgatcc gaccgcccag aaacgaccgg ccgcgaaaca cctcgatgcc ggagaacgac
      301 tcgccgatca ccccgaggaa ctgggcggtg acccatgggt caaacaccac ggtgagccgc
      361 tgagacgcga cccgcgaggc ccccaacagc cgcaatgccc gatcgacggc ctggtgtgcc
      421 gccgcctcgg cgtcgagcgc gtcgggccca cgacccaccg aaaacccgaa ccccgttgtc
      481 acctcgtcgt cgtcgccggc gaggctgtag acgatcaacg cgttggcggt ctccgaactg
      541 gcgcttcgaa tgccggtcgt ggatgccagt accgcggtgt cgatcgaatc gctgtattcg
      601 gccatctcga cgccgaggat gcgcggatct ccttcgagca cccgctgttc gagttcgatc
      661 gccaaggcga tcttctcctc cggggacatc gccgccacgc gagggtcgta aagatccatc
      721 tcgacgacct caaccccgtc ggggctcacg agtccgacga actcgtcacc ggtcgaaaac
      781 atcgcgttgt cgcgcgcctc ggccaacgtt tcaaacacaa cgtccgcttc gatcgatccc
      841 gcataggcga agccctgtcg cccgtcgtgc accacccgga taccgacacc gcgagactcc
      901 gacgaggtga acgcttcgac gtccccatcg taggcgcgca cctcggtctc gcgggcgtgg
      961 ctgaccacca cttcgatctg ttcgccaccg cgagctgcat cgagcacgct gtcggccacc
     1021 tgaaagaggt cgtcgggagt catggcggac agattgccac aggcgggctg ggtaccctgt
     1081 gacatgctct tcggcctccg tttcgacctc cgcaacccgg cgttcgccaa tacgacgatg
     1141 accgaacgtt atgaggcggc gctcgagatg gcgcagtggg ccgacgaacg tggggcggcg
     1201 ttcatctgtg tgtccgaaca tcacagttcg cccgacggct atctcccttc ggctctgaca
     1261 atggcggcgg cgatgatcgc ccgcactcga accacccgca tttgggtgaa cgccatcgcg
     1321 gcaccgctac accatccgat tcacctcgcc gaacaatgcg cggtgctcga caacctcagt
     1381 ggcggccgtt tcgacctcac ggtcggcgcg ggctacgtgc gaggcgagtt cgagatgttc
     1441 ggggtcgcga tgaaccggcg ccccagtctc atgaccgaaa ccgtcaccgc ccttcgacag
     1501 gcctggagtg gcgagccgtt cgagttccga ggtcgcaccg tgcgtgtcac cccaacgccg
     1561 gagtcgaagc gagggcctgg tctcacgatg ggaggcagca gcgaggctgc ggcacgtcgg
     1621 gcagcacgcc tcggcgtcgg gttcatcccc tccgagcctg cgctgtggga gttctatcgt
     1681 gatgaactcc tccagctcgg gctgcgcgat cccggcccgt tcgtctctgg agcagacacg
     1741 agcgtggtca tcctcgccga cgatgtcagc gccggctggc gggaactcgc cccctacttt
     1801 ctccatgaga cgaacgccta tggccagtgg cgcgaacccg acgatgtcgc gacgtcctat
     1861 cgcccggtgg aaacggtgga ggaccttcag gaccttggcc agtaccgggt gctgacaccg
     1921 gatcagtggc gcgacgaatt ggaggcggcc gggccgtttt cgctggccct cctccacccg
     1981 atggtcggcg ggatcccgcc gaaacggggc tgggaacatc ttcgaatgtt cgaacgcgac
     2041 gtcctcgact tcgatgccgc caagcccgaa tgaggatcga acgacgaaca tttctcaagc
     2101 gggcgagtgc ttcggcgatc gcgctcggcg ggctcaacgg agcctacgaa gtcggtcggc
     2161 gccgagggca cagccgggca cgcgaggcgg ccttagccct agcgtcgtca ggtccgagca
     2221 ccgagatggg cttgccgatc ggagggtttc gcacgatctt ttcggcacgc accgacgcca
     2281 acgtcctctc gctcaccttc gacgatggtc ccgatccgga gttcacagaa cacgtcctga
     2341 ccgtgctcga tcgctacgac gtcggtgcga cgttttgcat gatgggacac aacgcgctcg
     2401 aacatcggtc gctcgcacaa tccgtcgtgt cccacggcca cgaggtcgcc aatcattccc
     2461 tgacccatct cgatcaaacg cgactgtcgc ctcgcgagct tcacgacgag atcgcgctcg
     2521 gacaccaagc cgtcgtggaa gcgaccggct ctcccgtgcg ttacttccgt ccgccgcgcg
     2581 gtcgactcgt cggacgggcg agccgggttc tcgccgagct caacagcgac gcgttgttgt
     2641 ggtcgatcag cggttcgctc cgtgccccca cgacgaccgc gaagctgacc aaattggtca
     2701 tcgatgccct tcaccccgga gccatcgtgt gctttcacga tgggatcggt cgcggaacgt
     2761 tcgcccgaga gcagcgcttc gcccgggaac tgaaggcgcg ccgcaacgcc gagatcgagg
     2821 ccctccctgc aatcatcgaa gcggtcatcg accgcggcta ttcgttcgcg acggtcactg
     2881 agctgttggg cttcgaagcg acgaacccat acgagtcgga gatcacctca ctcgacgagc
     2941 ctcagatcgt cgatggagag gactcggtca tcgattggga cgacggttcc gacgacgtcg
     3001 actcaccgtt gaacgcggcc gaccccgaac ccgtcgctgc cgaggagaca gcgcttgagt
     3061 ccggggtcgg atgaagcgaa cgcgtgcttg gttctgtccg attcactcgg ctttgcggat
     3121 gtgcgcgagg ccggcgatca cgagcaccgc catcagcaac gctgcaccaa aggcaaccca
     3181 ggccgcgatc gccgcgatct gaccgatcgt cgcccacgcg taactcgtca acaacaggcc
     3241 acgaagcgtc tcgcccttga acaagctgtc gcgcagtgac gtcagacggt cggcctcggc
     3301 ctgcaattcg tcaatcaccg cctcgtcctc gccgttctcg atcgcttctt gcaccgcagc
     3361 ccttgccgcg cggtcctcga tctggctgta ggtcttgcca tcggccatac cgtcaaggtg
     3421 gccctggata tagctggcgt agcgctcggc gtgagaaccg gtggtgacct gcaggccagc
     3481 gaactccagc aggtcgtcac gtccttccgc cttcaatgca tccgcatcgg gaaagaagac
     3541 gttttgtgaa gcgagttcat cacgaacgta gtcattcgag aaattggcgc cccacgtcag
     3601 caacgcgccg gcgacgatca gcaccaccgt caagagtgca cccaccccga tcaacaagcg
     3661 atcgatggtc cgtcgggtca gtcggatctc tggttcgcca gacatcgtgt ctccttagag
     3721 gatgcgcggg tagatcaggt tggtgggtcc cagcggttgc gccagtcgat cagtttggcg
     3781 gcgaggatga acacgatcgc taacgcgagt tgggcttcgc ggtgggcgcg tttgcggtcg
     3841 gtgtttcggc ggagttggcc gtagttgacc agccaggagt tggtgcgttc gacaggccaa
     3901 cgcaggccga gaccgacttt ggtgtggggc gggtcgcctg gtttgcgtcg tttcgcgatg
     3961 accgcgtcgt caagcccgta gccggccagg gtcgctcgaa cggtgttggc gtcataaccg
     4021 cggtcaaggt ggatcgtgtc gatgtcggcg aggaggtgtc ggccagcgat cgagtccaac
     4081 gtgggggcca atagtggaat gtcgtgacgg ttggcgccgt cggtcgccca gccgaccggt
     4141 atcccgttgc ggtcggtacc gatggaccat ttccagccgg agcgtccccg gtcggtgggg
     4201 ttggggccgg tgccgtcgcc gccttggggg gctttgtgga gggagccgtc aacagctact
     4261 tcgctgaggt ccaagccgat gatgcggtca taggcgtcga gggcgtgttc gaacgctcgt
     4321 cggaacacgt cgtggcagac ccatagtttg aaccgtgctc gggcggtggt gtcggaaact
     4381 tgccagccgc aaagcgcttc ggcgtcgacc catgagcagc cacaaacgag gcggaccagg
     4441 atcaggtgga acacgattcg gtctggggtg cggggccggt ggccgccttt gggatggttg
     4501 tcgacgtgtt cgggaaggag cggttcgaaa ctcttccaga cggcatcgat gacttcgcgg
     4561 tccagagcgc gcatactggt ggggtcctcc ggtttggttg ggttggtgca cccgcgattt
     4621 catcacgggc aaccttccaa ccggtggacc accaacactg tcgggcgaga cacggcgctt
     4681 cgcagcgcac accccgatct atccgcgcga cctctta
//
LOCUS       DAOOMA010000139         4716 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_21848_length_4716_cov_4.303583, whole genome shotgun sequence.
ACCESSION   DAOOMA010000139 DAOOMA010000000
VERSION     DAOOMA010000139.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 4716)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4716)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4716
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21848_length_4716_cov_4.303583"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2056)
                     /gene="polA"
                     /locus_tag="PLP95_05085"
     CDS             complement(<1..2056)
                     /gene="polA"
                     /locus_tag="PLP95_05085"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020376885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HPB45211.1"
                     /translation="MTTLMLIDGNSLTYRAFYALPTDMATASGQVTNAVFGFTSMLIN
                     LVRDHQPDLLAVTFDRPEPTFRHKMVESYKAQREASPDILRQQMGLVRQLVETLKIPI
                     LEQAGVEADDIIATLATQAADDGIDVIVVTGDRDSYQLVADPHIRVLYNKRGVSDYAL
                     YDEAGIEERTGVKPSQYVYYAALRGDPSDNLPGVPKVGEKTAAKLVTAYGDMDGIYAH
                     TADQTPALRKNLEENEAQARSNVEMMTLKRDVELDLAPSELRQGDVDTDAVRDLFKFL
                     EFGTLMGRLGAAFGDTTTAELDTARNVIEAEVHEVSGDEAAALLRSFADRSEPIVLAP
                     SWHSQPGRSDLAGLAVVSDRDGAEVSWFGAEVLAAHGDALDDLLANGPEVIAHDIKPL
                     LRRWLTEGRPLPRLAMDTKIAAYLLDPADTRYGIAELVERYTDAELAQGDATPAGQLD
                     FDAGSEPVGTAAAREALATAFVATPITEALEAQGLSSLADTIEYPLITALARMEFLGI
                     GVDRDALAALNEHFTSEAARLRGAVVAEAGEEFNVNSTPQLRTILFDKLGLTTQKKTK
                     TGYSTDAASLEKIRDQHPIVEYLLQYREMEKLRSTYGTGLLAEVGPDGRIHATFNQTV
                     ARTGRLSSDQPNLHNIPVRSEEGRRFREVFIPSPGSVFIVADYNQIELRCIAHLAHDP
                     GLI"
     gene            complement(2158..2751)
                     /locus_tag="PLP95_05090"
     CDS             complement(2158..2751)
                     /locus_tag="PLP95_05090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HPB45212.1"
                     /translation="MSEPRAPLRIVVAEDDAIIRMDLCAILEEQGYEVVADVGQGDLA
                     VEAVRQHEPDVAVLDVKMPVMDGITAAGEIASERLCAVVLLTAFSQRKLIEDARDAGV
                     MAYLVKPFQPEELMPAIEMAVGRYVEMAALADRSATLEEQLETRKLTDRAKAVLMNDH
                     GLSEVQAFRFLQRGAMDRRSTMKAIAQAVVDGELSPS"
     gene            complement(2748..3047)
                     /locus_tag="PLP95_05095"
     CDS             complement(2748..3047)
                     /locus_tag="PLP95_05095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB45213.1"
                     /translation="MSWFVERRLRSVAQNLRSARDDLAVTDEQLDQLVDEAEDAALRS
                     IVSDDRSAVLDSNDAIRHRDALVRHRQGLVDKIASLEARQDELLDEMNQRRSGRS"
     gene            3406..3603
                     /locus_tag="PLP95_05100"
     CDS             3406..3603
                     /locus_tag="PLP95_05100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB45214.1"
                     /translation="MTEEPFERVLTREEFNEMLRDAPPPTSDDVSITSDGRRLDSKEA
                     VIAFFTQLEAELAGDRTTADS"
     gene            3614..4138
                     /locus_tag="PLP95_05105"
     CDS             3614..4138
                     /locus_tag="PLP95_05105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB45215.1"
                     /translation="MTEGPPLDRARIIECLDDHGVEYLVVGGIGAQLHGATRPTADFD
                     SLPSTSKENLGRLAAAMKELNAYLRVEGLTDEEARALPTRLDEESLGRMDISTWRTDA
                     GDLDVLTAIPTRDGGRAFYDDLSRRATRMQVAGAVVWVASLPDIIASKEWADRPKDRE
                     ALPELRQLSEEAGE"
BASE COUNT          761 a         1560 c         1553 g          842 t
ORIGIN      
        1 cgatgaggcc cgggtcgtgt gccaggtggg cgatgcaacg cagttcgatc tggttgtagt
       61 cggccacgat gaacaccgat ccgggtgacg ggatgaacac ctcgcggaag cgtcgacctt
      121 cctcggaacg caccggaatg ttgtgcaggt tcggctgatc cgaactcagc cgaccggtgc
      181 gggccacggt ctggttgaag gtggcgtgaa tcctcccgtc cgggccgacc tcggcgagca
      241 gcccggtgcc gtaggtgctg cgcagcttct ccatctcccg gtactgcaac aggtactcga
      301 cgatcggatg ttgatcgcga attttctcca gcgaggcggc atcggtggaa tagcccgtct
      361 tggtcttctt ctgagtggtc aggccgagct tgtcgaacag gatggttcgc agctgcgggg
      421 tcgagttcac gttgaactcc tcccccgcct cggccaccac cgcgccccgc aggcgcgccg
      481 cttcggaggt gaagtgttcg ttgagcgccg cgagcgcgtc gcgatcgacg ccgatgccca
      541 ggaactccat gcgggccaac gcggtgatca aggggtattc gatcgtgtcg gccaatgaac
      601 tcaggccttg tgcctcaagg gcctcggtga tcggcgtcgc gacgaacgcc gtggcgaggg
      661 cctcgcgcgc cgctgcggtc cccaccggtt ccgaaccggc atcgaaatcg agttgcccgg
      721 cgggggtggc gtcgccctgg gccagttcgg cgtcggtgta tcgctcgacc aactcggcga
      781 tgccgtaccg ggtgtcggcc gggtcgagca ggtaggccgc gatcttggtg tccatcgcca
      841 atctcggcag tggacggccc tcggtcagcc accggcgaag cagcggcttg atgtcgtgag
      901 cgatcacctc cggaccgttc gccaacaggt cgtcgagggc gtcgccatgc gcggcgagca
      961 cctcagcgcc gaaccacgac acctcggcgc cgtcgcggtc gctcaccacc gcgagcccag
     1021 ccagatcgct tcgaccgggc tggctgtgcc acgagggcgc caacacgatc ggttcggaac
     1081 ggtcggcgaa gctccgcagc aacgcggcgg cctcatcgcc ggacacctcg tgaacctcgg
     1141 cctcgatgac gttgcgtgca gtgtcgagtt ccgccgtggt cgtgtcgccg aacgctgcgc
     1201 ccaaacggcc catcaacgtg ccgaactcga ggaacttgaa caggtcgcgc accgcatcgg
     1261 tgtcgacgtc gccttgccgt aactccgacg gcgcaaggtc gagttcgacg tcgcgcttga
     1321 gggtcatcat ctcgacgttg ctgcgggcct gagcctcgtt ttcctcgagg ttcttgcgaa
     1381 gggccggggt ctgatccgcg gtgtgcgcgt agataccgtc catgtcgccg tacgcggtca
     1441 cgagtttcgc cgcggtcttc tcccccacct tgggcacgcc gggcagattg tcggaggggt
     1501 cgcctcgcag cgccgcgtaa tagacgtact gcgatggttt gacgccggtg cgctcctcga
     1561 tgccggcctc gtcgtagagc gcgtagtcgc tgacgccgcg cttgttgtac aacacgcgga
     1621 tgtggggatc cgccacgagt tggtagctgt cgcgatcgcc ggtcacgacg atgacgtcga
     1681 tgccgtcatc ggccgcctgg gtggccaggg tggcgatgat gtcgtcggct tcgacccccg
     1741 cctgttcgag gatcgggatc ttcaacgtct cgacgagttg gcgcaccagc cccatctgct
     1801 gtcgcaagat gtcggggctg gcctcgcgct gggccttgta ggactcgacc atcttgtggc
     1861 gaaaagtcgg ttcggggcga tcgaaggtga ccgcgagcag gtcgggctgg tgatcgcgca
     1921 cgaggttgat gagcatcgag gtgaacccga acaccgcatt ggtgacctgc cccgaggccg
     1981 tggccatgtc ggtcggcagg gcgtagaagg cccggtaggt gagggagttc ccgtcgatca
     2041 gcatcagcgt cgtcatcggc gccgatgcta ctcatgaccc ccgacccagc ctgttctccg
     2101 ataaccagtg gtcgtgatca cgaccactgg ttatcggaga acggatgtgg cggcgggtca
     2161 ggacggggag agttccccgt cgaccaccgc ctgggcgatt gccttcatcg tcgaacgtcg
     2221 gtccatcgcg ccccgctgca gaaagcggaa cgcctgcacc tcgctgagcc cgtggtcgtt
     2281 catcaacacg gccttggcac gatcggtgag ctttcgggtc tcgagttgct cctcgagcgt
     2341 cgccgaccga tcggcgagcg ccgccatctc gacgtagcga ccgaccgcca tctcgatcgc
     2401 cggcatgagt tcctcgggct ggaacggctt cacgaggtac gccatcaccc cggcgtcgcg
     2461 ggcgtcttcg atcagcttgc gctggctgaa cgcggtgagc agcacgactg cgcacagtcg
     2521 ttccgaggcg atttcacccg ccgcggtgat gccatccatg accggcatct tgacgtcgag
     2581 gacggcgaca tcgggctcgt gttgtcgcac ggcctccaca gcgaggtcgc cctggccgac
     2641 atcggccacc acctcatagc cctgctcttc gagaatggcg cacaggtcca tccggatgat
     2701 ggcgtcgtcc tcggccacca cgatgcgcaa cggtgcgcgc ggttcgctca tgatctcccc
     2761 gatcggcgct ggttcatctc gtcgagcaac tcgtcctggc gcgcctcgag cgaggcgatc
     2821 ttgtcgacga ggccctggcg atgacgaacg agcgcgtcgc gatgacggat cgcatcgttg
     2881 gagtcgagca cggcggaacg gtcgtcgctc acgatcgacc gcagcgcggc gtcctcggcc
     2941 tcatcgacga gttggtcgag ctgttcgtcg gtcaccgcca gatcgtctcg ggcactgcgc
     3001 aggttctgtg cgacggaccg taaccggcgt tccacaaacc aagacatgcg gcccaagtgt
     3061 aggcgacgcc tttgcgcgca cggatcggac gggggctccg gcgggttcgc acgacgcgac
     3121 tcaccccgaa cgtttcggtc cggggtgagg tgatcgtgcg tgctcgcttt tggtgcccgg
     3181 ggtgggactc gaactccccg gcagaacgta cctactggta cctccccgtg cgaatcagta
     3241 ccgctgaaca ggagcttcgt acgggacccg tgtcagccga tgctggccgc tgacggcccg
     3301 atccaccacg ttcgtgacca actcgtgacc aacggcgcgc cgcgccagag gccgtgaccg
     3361 gtcgtccacg ccttcttggt gagtggccgg gcatagcatg gagccatgac cgaggagccg
     3421 ttcgagcggg tgctgacccg tgaggagttc aacgagatgc tccgcgacgc gccgccgccc
     3481 acgtccgacg acgtgtcgat caccagcgac ggccgcaggc tcgactcgaa ggaagcggtc
     3541 atcgcgttct tcacccagct cgaggcagag ctggccggcg accgcaccac cgccgactcg
     3601 taggcgcgcc gacgtgacag agggtccgcc gctggatcga gcccggatca tcgagtgcct
     3661 cgatgatcac ggcgtggagt acctggtcgt tggcggcatc ggtgcccagc tccacggagc
     3721 gacccgcccg accgccgact tcgactcgct gccgtcgacc tccaaggaga acctcggccg
     3781 cctcgctgcc gcgatgaaag agctgaacgc ctacctgcgc gtcgaaggcc tcaccgacga
     3841 ggaggcccgc gcccttccca cgaggctcga tgaggagtcg ctcgggcgca tggacatctc
     3901 gacctggcga actgatgccg gtgatctcga cgtgttgacc gcgatcccca cgagggacgg
     3961 tggccgcgcc ttctacgacg acctctcgcg gcgggccaca cggatgcagg tggcgggagc
     4021 cgtggtgtgg gtcgcctcgc tcccggacat catcgcctcg aaggagtggg ccgatcgccc
     4081 caaagaccgg gaggccttgc ccgagcttcg gcagctcagc gaggaggcgg gtgagtagcc
     4141 gtagcgctgg tgctgaaggc caagatcccc gccgagttcg acacccactg cgagctcgag
     4201 ccacggccta ctacccaccg gacccagact cgcctggcga gccgacgacc cggcacctct
     4261 cagtcatcca taagatcgcg ttcgccgatc tcgccgtaga tgtgcggcca gaggtacggc
     4321 cccatcctct cgcggtggtc agcgtatgag ggctggccat caagccaggg ccagctgcga
     4381 tcagctaacg cccgatctgc tatgggatcg agaccgagtt cgatccgcat ctcattccgc
     4441 agtagctcgc gcaggccctt cgccatctgc tgggccggcc aatggtcgct gtagatggca
     4501 ggcgtgatcc tgctttcgcc tggtccgagg tgttggggct gagggactgc tgcaagcacc
     4561 ccatgagccg ctcttgcagc gacatccgcg accctcggtg tggcaaagta gctcaatttc
     4621 gctgagcatc ggctggagct ccatgtccca gcactccatc cactcctggg acatctcccg
     4681 aacgagtcgt tcgtcgtcgt cctccatcgc cggcgc
//
LOCUS       DAOOMA010000140         4712 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_21872_length_4712_cov_3.593730, whole genome shotgun sequence.
ACCESSION   DAOOMA010000140 DAOOMA010000000
VERSION     DAOOMA010000140.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 4712)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4712)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4712
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21872_length_4712_cov_3.593730"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            83..2434
                     /locus_tag="PLP95_05110"
     CDS             83..2434
                     /locus_tag="PLP95_05110"
                     /inference="COORDINATES: protein motif:HMM:NF014215.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HPB45216.1"
                     /translation="MGVMSLRSTRTATVAMAVAALLMTGACTNTDEGGTDSDTERIEL
                     IDSATASFVVTGSVNQVFVSDAKPEEELLLVDADGAVVDTATTDDQGSLIYRLVDEGD
                     NMRVVSAQDNTVQSETIDVLDADESQPDQSFYDNQTLTEGFHYITTRDGTTLSAAVYL
                     PPGDGPFPTVVEYSGYDPSDPTQNLRGTVEDLGLNPDDLCSTIKIICAKPAQPSSLLA
                     AAAGYAVVAVNVRGTGCSGGAYDFFEPLQLLDGYDVIETVAAQEWVRGKVGMVGLSYP
                     GISQLFVASTNPPNLAAITPMSVFHDTARGVLSPGGIYNEGFALSWADNVLDDAVAFG
                     QGWTETVTEGGDTTCAANQRFRGQNVDATKKALANQYYTDDVAMPLDPSTFVDRIDVP
                     VFMTGSWQDEQTGPSFSRLWDRFTKAPVTKFMAMNGAHGDGYSPETYAELKMFLDFYV
                     AGQRTPLPDFLRSLGPSLLADTFGVTVQYPPERLIDGEFDDLRAQYEAEPDVTVLWDR
                     GGDPSNLGAPESLGRTQFDTWADRDVAAQRWFLGPGGRLDDAKSTASGGFTSFNTDLA
                     RAQRTTLPGDRIDEAFDALPTYDWVQDSEGAAAVFQTEPLERDTVILGGGSADLWIRA
                     DGEEAHVGVTLSEVRPDGTEVFIQVGQLKASRRAPGPNATEFDPDHTLRREDVEALKP
                     GEWTEARVEIFPVGHVLRAGARLKLSVHTPGGDQARWAWIIDDTEPASIDIGTDAAHP
                     SSIVLPIGTAVTGYRPEFPACPGLRAQPCRAAQAFTNTDRNGD"
     gene            complement(2470..3087)
                     /locus_tag="PLP95_05115"
     CDS             complement(2470..3087)
                     /locus_tag="PLP95_05115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB45217.1"
                     /translation="MGAMQRVDDLQQHLLESVAEAQRGVVTVQQVRQLLGSASAELRF
                     RSDPRWEQVSSRLHRRVDSESTLEQRCVIAVLELGGCTLVSHRSAAMLYGLYERDDQL
                     VHVTTTSKRRQHGGVARLHRVDEVPLEQQRVLGGVPIVSPALLAMQAFGSLPSDDAVD
                     LTTTMMNLGMLERHELEELLERWGERGRNGAGGLRAFLGRRPMAV"
     gene            complement(3181..4410)
                     /locus_tag="PLP95_05120"
     CDS             complement(3181..4410)
                     /locus_tag="PLP95_05120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HPB45218.1"
                     /translation="MSIDFTLTPELDDLRLRVRAFIDEVVKPGEARIGNPDDIERDEY
                     VRLLFSMRDQARAAGLWLPHMPVEWGGMGLGHVELAMVQAEAAKTYYGPFVLNCMAPD
                     EGNMHTLLHWATDAQKEKYLRPLCEGMAMSCFAMTEPEVAGSDPTLIRTTAVQDGDEW
                     VINGHKWFISNARRAQFAILVCRTEDNPDLPQAANTAFLVDIPSEGWNRVREVETMHG
                     GSGHSEIVIEDLRVPAANMLGGRGQGHLLGQYRLGPARLAHCMRWIAQAETALDMMVD
                     RSLNRYAHGSILAEKQGIQWMIADSTMELYQSKLMVLHAAYRIDNGLEFKSEVSMAKH
                     FVANMLNRVIDRSIQVHGALGYSTDTPLAHMYQHARWARFADGADEVHQMRIAQRTIA
                     AFRDEGSTRRATGDLPL"
     gene            4490..>4712
                     /locus_tag="PLP95_05125"
     CDS             4490..>4712
                     /locus_tag="PLP95_05125"
                     /inference="COORDINATES: protein motif:HMM:NF013633.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein"
                     /protein_id="HPB45219.1"
                     /translation="MADSAGLDSVRVDSYLWAIRLYKTRSSATDAVKGGHVRVNGHPA
                     KPATKLKVGDRIECRAQGRTFVVEAVRLLD"
BASE COUNT          793 a         1577 c         1556 g          786 t
ORIGIN      
        1 gacccacgtc gtgcccaagt agtgtcgcct gcggccgccg gagacacctc cgagcgcagt
       61 ccgatctcga gggggagagc cgatgggcgt gatgagtctg aggtcgacgc ggacggcgac
      121 ggtcgccatg gcggtggcgg cgctgttgat gaccggtgcg tgcaccaaca ccgacgaggg
      181 tggaaccgac tcggacaccg aacgcatcga gctcatcgac tcggcgacgg cctcgttcgt
      241 cgtgacgggt tcggtgaacc aggtcttcgt cagtgacgcc aagcccgagg aggaactact
      301 tctcgtcgat gccgacggcg cggtcgtcga caccgccacc accgacgacc agggatcgct
      361 gatctatcga ctcgtcgacg aaggcgacaa catgcgggtc gtcagtgcgc aggacaacac
      421 cgtgcaatcc gaaacgatcg acgtgctcga cgcggacgaa tcgcagcccg atcagagttt
      481 ctacgacaac cagaccctta ccgaggggtt ccactacatc accacccgcg acggtacgac
      541 cctgtccgcc gcggtgtatc tgccccctgg ggacggcccc ttccccaccg tcgtggagta
      601 ttccggctac gacccgagcg acccgaccca gaacctgcgc ggcaccgtgg aggacctcgg
      661 cctcaacccc gacgacctct gttccacgat caagatcatc tgcgcgaagc ccgcccagcc
      721 gagttcgttg ctcgctgcgg ccgccggtta cgcagtggtt gcggtcaacg tgcgcggcac
      781 cggatgttcg ggcggggcct acgacttctt cgaaccgctg caactcctcg acggctacga
      841 cgtgatcgaa accgtcgccg cccaggagtg ggtgcgcggc aaggtcggca tggtggggct
      901 cagctacccg ggcatctcgc agcttttcgt cgcgtccacc aatccgccga acctcgcggc
      961 gatcaccccg atgtcggtct ttcacgacac cgcccgcggc gtgttgtcgc ccgggggcat
     1021 ctacaacgaa gggttcgcgc tgtcgtgggc ggacaacgtg ttggacgacg ccgttgcgtt
     1081 cggccagggc tggaccgaga cggtgaccga aggtggcgac accacgtgtg ccgccaacca
     1141 gcggttccgc ggccagaacg tcgatgccac caagaaggcg ctcgccaacc agtactacac
     1201 cgacgacgtg gccatgccgc tcgacccgtc gaccttcgtc gatcggatcg atgtgccggt
     1261 gttcatgacc gggtcgtggc aggacgaaca gaccggcccg agtttctcgc ggctgtggga
     1321 ccgattcacc aaagcgccgg tcacgaagtt catggcgatg aacggggctc atggtgacgg
     1381 ttactccccc gagacctatg cggaactcaa gatgttcctc gatttctacg tcgctgggca
     1441 gcgcacgccg ttgcccgact tcctgcgctc cctcgggccc tcgctgctgg ccgacacctt
     1501 cggggtgacg gttcagtacc cgccggagcg tctgatcgac ggggagttcg acgacctgcg
     1561 cgcgcaatac gaggccgagc ccgatgtgac ggtgctgtgg gaccgcggcg gcgacccgtc
     1621 caaccttggc gcccccgaat cgttgggccg aacccagttc gacacgtggg cggatcgaga
     1681 cgtcgctgcg caacgctggt tcctcggccc gggcggccga ctcgacgacg ctaagtcgac
     1741 ggcgtccggc ggcttcacct ccttcaatac cgacctcgcg cgcgcccagc gaaccaccct
     1801 gccaggcgac cggatcgacg aggccttcga cgcgctgccg acctacgact gggtccagga
     1861 cagcgagggg gccgcggcgg tgttccaaac cgaaccgctc gagcgcgaca cggtgattct
     1921 cggcggaggc tccgcggatc tgtggatccg ggccgatggc gaggaggcgc acgtgggcgt
     1981 cacgctcagt gaggttcgcc ccgacggcac cgaggtattc atccaggtcg gccagctgaa
     2041 ggcctcgcgg cgagcacccg ggccgaatgc caccgaattc gatcccgacc acacgcttcg
     2101 ccgtgaggac gtcgaggcgc tgaaacccgg cgagtggacc gaggcgaggg tggagatttt
     2161 cccggtcggc catgtgctgc gcgccggcgc ccgcctgaag ctgagcgtcc acacccccgg
     2221 cggcgatcag gcgcgttggg cctggatcat cgatgacacc gaacctgcga gcatcgatat
     2281 cggcaccgac gcggcgcatc cctcctcgat cgtgttgccg atcggaactg cggtcaccgg
     2341 gtatcgcccc gagttcccgg cgtgtcccgg attgcgggca cagccgtgcc gcgctgccca
     2401 ggcgttcacc aacaccgatc gaaacggcga ctgatcaacg cggccggagc gggccgcggg
     2461 caacgctact cacaccgcca tcggccgacg gccaagaaac gcccgcagtc cgccggcgcc
     2521 gtttcggccg cgttcgcccc agcgttccag gagttcctcg agttcgtggc gctcaagcat
     2581 cccaaggttc atcatcgtcg tggtcagatc gaccgcatcg tcgctcggca gcgaaccgaa
     2641 ggcctgcatg gccagcagcg ccggcgacac gatcggcacg ccgccgagca ctcgctgttg
     2701 ttcgagcggc acctcgtcga cccggtgcag gcgggcgacc cctccgtgtt ggcggcgttt
     2761 ggaggtggtg gtgacgtgca cgagttggtc gtcgcgttcg tacagcccgt acaacatggc
     2821 ggcgcttcgg tgcgacacca gggtgcatcc tccgagttcg agcaccgcga tgacacagcg
     2881 ctgttccagg gtcgactcgg agtcgactcg ccggtggagc cggctcgaca cctgctccca
     2941 gcgcgggtcg gaacggaacc gcagttccgc cgaggccgag ccgagaagtt ggcggacctg
     3001 ttgcaccgtc accacgccgc gttgggcctc ggcaacggat tcgagcaggt gttgttgaag
     3061 gtcatccacg cgttgcatgg cgcccatgaa aggcgacccg atgcggcgtg cctatggggg
     3121 acgtccccct ccccgacccg aggaccgcgg gcgggtcagc gagcgggcag gagctcgccg
     3181 ttacagcggc aggtcgccgg tggcgcggcg ggtggaaccc tcgtcgcgga acgcggcgat
     3241 cgtgcgctgg gcgatgcgca tctgatgcac ctcatcggca ccgtcggcga accgtgccca
     3301 acgggcgtgc tgatacatgt gggccagcgg cgtgtcggtc gagtagccga gggcgccgtg
     3361 cacctggatc gatcggtcga tcacccggtt caacatgttg gcgacgaagt gcttggccat
     3421 cgacacctcg cttttgaact ccagcccgtt gtcgatgcga taggcggcgt gcagcaccat
     3481 caacttggac tgatacagct ccatcgtcga atccgcgatc atccactgga tgccctgctt
     3541 ttcggcgagg atcgaaccgt gggcgtagcg gttcagcgac cgatcgacca tcatgtcgag
     3601 cgcggtttcg gcctgggcga tccaacgcat gcagtgagcg aggcgggccg gaccgaggcg
     3661 gtattgaccc agcagatgcc cctggccgcg gccgcccaac atgttcgcgg cgggcactcg
     3721 gaggtcttcg atgacgattt cggagtgccc cgatccgccg tgcatcgttt ccacctcgcg
     3781 cacccggttc cagccctccg acgggatgtc gacgagaaac gccgtgttgg ccgcctgcgg
     3841 gagatcgggg ttgtcctcgg ttcggcacac caggatggcg aactgtgccc ggcgggcatt
     3901 cgagatgaac cacttgtgcc cgttgataac ccattcgtcg ccgtcttgga ccgcggtcgt
     3961 acggatcagc gtggggtcgg atccggctac ctccggttcg gtcatggcga agcaactcat
     4021 cgccattccc tcgcacaacg gccgcaggta cttctccttc tgagcgtcgg tggcccagtg
     4081 caacaacgtg tgcatattgc cctcgtcggg ggccatgcag ttcaacacga atgggccgta
     4141 gtacgtcttg gccgcctcgg cctgaaccat cgccagctcc acgtggccga ggcccatgcc
     4201 accccattcg acaggcatgt gcggcaacca caaccccgca gcgcgagcct gatcgcgcat
     4261 ggagaacagc aggcgcacgt actcgtcacg ttcgatgtcg tcggggttgc cgatccgcgc
     4321 ctcgccgggc ttgacgacct cgtcgatgaa cgctcgcacc cgcagccgga ggtcgtcgag
     4381 ttcgggggtg agggtgaagt cgatggacat gtggcctcca caggtcgagt ggcgttgggt
     4441 tcgtgccacc gtaggccacg agcggtgccg gaccgctacc gtcgcgtgta tggccgattc
     4501 cgctggtctc gattcggttc gggtcgactc ctatctctgg gcgatccggc tctataagac
     4561 ccgctccagc gcgaccgacg cggtgaaggg cggccacgtt cgggtcaacg gtcacccggc
     4621 gaagccggcg acgaagctca aggtgggcga ccgcatcgaa tgtcgggcac agggccgaac
     4681 gtttgtggtc gaggcggtcc gtctgctcga ca
//
LOCUS       DAOOMA010000141         4693 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_21983_length_4693_cov_4.094222, whole genome shotgun sequence.
ACCESSION   DAOOMA010000141 DAOOMA010000000
VERSION     DAOOMA010000141.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 4693)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4693)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4693
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21983_length_4693_cov_4.094222"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..676)
                     /locus_tag="PLP95_05130"
     CDS             complement(<1..676)
                     /locus_tag="PLP95_05130"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HPB45220.1"
                     /translation="MLTGTTLWQLINDRVEATPDALLVVDEDGRTMTFAEYAVASERA
                     AAGLAALGVGEGSVVTWQLPTWIESMVLVAALSRLGATQNPVLPIYREREVGFCTKQA
                     GADLIVVPSEWKGFNYQQMATEIVNANGHGAVLVADKRLPDGDPSTLAPLADTDSDPV
                     RWLFYTSGTTSDPKGARHTDASVAAVAASMGRRMNVTAEDRSAVVFPFTHIGGITWLF
                     CALQFGH"
     gene            847..1617
                     /locus_tag="PLP95_05135"
     CDS             847..1617
                     /locus_tag="PLP95_05135"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020376783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HPB45221.1"
                     /translation="MDRETFGSMFDLTGRVAIVTGGTRGIGLAIAEGFLLAGARVVVS
                     SRKADACAAAQEHLESLGGDVLGVPAHMGDLNALQNLVDVTIDQFGRLDIVVNNAANA
                     LAQPAGHFTADAYQKSFDVNVRGPVFLTQYALPHLEASGHGAVINMVSAGAFLFSEHL
                     ALYSAGKAAMVSFTKSLAGAFAPKNIRVNAIAPGPIDTDMVRANPIEFQQAMGASTLL
                     NRIASADEVVPTALLLASDAGSYITGQVFSVDGGLVPR"
     gene            complement(1844..2791)
                     /locus_tag="PLP95_05140"
     CDS             complement(1844..2791)
                     /locus_tag="PLP95_05140"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF016115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HPB45222.1"
                     /translation="MAISEHVLATAAKVSNWGRWGDDDELGCGNLLTPEAAQRGAACV
                     RTGKRFSLAVDLRNDGIQVGQPAGRYNPILTFTSMNERDKMAPGIWEGCDDLVMMSTC
                     AATHIDALSHISYDDLLYNNRPKRSIGAHNGATWCGAEKLPSVVTRGILLDVAKLHGS
                     DRVDHGHAVTAEDLDRALEATGLSIEPGDAICVRTGDLKWYFEGDRRRYAVGKDWRTV
                     GFGFSCVEWMADHDVAGAFIDSYTYEVMPPESGNWDDLLCVHMVQLRDMGLLQGQNWN
                     FESLAEDCAEDGVYEFLLVAAPEPFVGATSAPVVPIATK"
     gene            complement(2798..3883)
                     /locus_tag="PLP95_05145"
     CDS             complement(2798..3883)
                     /locus_tag="PLP95_05145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-dependent alcohol dehydrogenase"
                     /protein_id="HPB45223.1"
                     /translation="MKALVFDGKTATVRNDVTIRGVRPGEVRVRIAAAGLCHSDVSVL
                     NGTIQFPPPVVLGHEGAGEVIEVAPDVTNVAVGDHVVMSTLGNCGQCAQCDRGKPTFC
                     RATLGKLNRPYLIGEGEDAKKGFQFANLGVFAEETVIKATQAVRIPNDVPLTTACLIG
                     CGVLTGVGAAFNRAQVAQGESAVVIGVGGIGLNALQGAALSGALPVIAVDTNPGKEAF
                     ARQFGATHFICPDGPDFDLVAAVKEICPNGVDHVFECVGSTALIKTSTELLDWGGKLV
                     MLGVPKMGSEASFVVNTMYNDKTIMGCRYGSARPQYDIPLMVRLYEAGRLKLDELVSA
                     TYPIEDFQKALDELHEGKLARGVLTMG"
     gene            complement(3944..>4693)
                     /locus_tag="PLP95_05150"
     CDS             complement(3944..>4693)
                     /locus_tag="PLP95_05150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB45224.1"
                     /translation="RRPKVLFVLCSVGLVVAGTAFWWEATREVDTVAVRDGSPEITSY
                     GSDGSGNWECGPHRGEGETPTWAVSAEPGIEEAVAAVANARIAPVIGRGFLAEPGTIS
                     FGGGSCGVGIRLAGAQHHGWMQVYDGEDGDWRFGGISAGRTAFGDGSMPWAAEITSTV
                     SRSTISVLPPVSAIQGVEVAPGEMLVWFAQSRSVASLTADPEASEADGARWTGVLPDG
                     RNPGEDAWLLITWVDDVGALVGFEMVPQPVN"
BASE COUNT          828 a         1607 c         1470 g          788 t
ORIGIN      
        1 tgtggccgaa ctgcagggcg cagaacaacc aggtgatgcc tccgatgtgg gtgaacggga
       61 acacgacggc gcttcgatcc tctgcggtga cgttcatccg ccgccccatc gacgccgcca
      121 ccgcggcgac cgatgcatca gtgtggcggg cacccttcgg atccgatgtc gtgccggagg
      181 tgtaaaagag ccaccggacc gggtcgctgt cggtgtcggc cagcggcgcc agtgtcgacg
      241 gatcgccgtc gggcaaccgc ttgtcggcca ccagcacggc accatggcca ttggcgttca
      301 cgatctcggt ggccatctgt tggtagttga accccttcca ttccgagggg acgacgatga
      361 gatcagcgcc ggcctgcttg gtgcagaacc cgacctcgcg ctcgcggtag atcggcaaca
      421 ccgggttctg ggtggcgccg aggcggctga gcgcggcgac gagcaccatc gactcgatcc
      481 acgtgggcaa ttgccaggtc accaccgagc cttcgccgac gccgagcgcc gcgagtcctg
      541 cggcggcccg ctcggacgcc acggcatact cggcgaaggt catggtccgg ccgtcctcgt
      601 cgacgacgag cagcgcgtcg ggagtcgcct caacccgatc gttgatgagc tgccacaacg
      661 tggttccggt caacatggct ccccctctcg ccgcgaacga tcggcggcgc tgaatgcgat
      721 ctgacggatc gtcagcttag ccccgccagt tccgcgcccc cgcttgttct ccgataacca
      781 gtggtcgtga tcacgaccac tggttatcgg agaacaagcc tgaggggcgg catagagtga
      841 gcggccatgg atcgcgaaac ctttggttcg atgttcgacc tgacgggacg tgtggccatc
      901 gtcacgggag gaacccgcgg catcggcctc gccatcgccg aggggtttct gttggctggc
      961 gcccgagtgg tggtgtcgag ccgcaaggcc gacgcctgtg ccgccgcgca ggaacacctc
     1021 gaatccctcg gcggcgacgt gctcggagtc cccgcgcaca tgggcgacct gaacgcgttg
     1081 cagaacctcg tcgatgtcac catcgatcag ttcggacgcc tcgatatcgt ggtgaacaac
     1141 gccgcgaacg cgctcgctca accggccggc cacttcaccg ccgatgcgta ccagaagtcg
     1201 ttcgacgtca acgtccgcgg gccggtgttt ctcacccagt acgcactccc gcatcttgag
     1261 gcgtcgggac acggcgcggt gatcaacatg gtttcggccg gagcgttctt gttctctgaa
     1321 cacctcgcgc tctactcggc cggcaaggcc gccatggtga gtttcaccaa gtcgctcgca
     1381 ggagcgttcg cccccaagaa catccgcgtc aacgccatcg cccccggacc gatcgacacc
     1441 gacatggtgc gagcaaaccc aatcgagttt caacaggcga tgggagcttc cacactgctc
     1501 aatcgcattg catcggccga cgaggtcgtg ccgacggccc tactgctcgc gtcggatgct
     1561 ggctcctaca tcaccggtca ggtattcagc gtcgacggcg gtctggtgcc ccgctgagcc
     1621 gacgaccgga tcgcccggat cggacagggt cgccagcgcg ccgaggcccg aagtcaggag
     1681 tgccgcgatc gccaccgccc cgagaaccgc gcgtcgccgt tgcttcggag ttggagcgag
     1741 gagttcgtcg gcgtagccgt gcatgcgcaa aagccttgca ccctggaccc cggttggaaa
     1801 gcgaaagaaa tgtgatggcg acgcgcgcca ccggcgcgag ccgttacttg gtggcgatcg
     1861 gcacgaccgg cgccgaggtg gctccgacga acggctcggg tgctgcgacc agcagaaact
     1921 cgtagacccc gtcttcggca cagtcctcgg ccaggctttc gaaattccag ttctgaccct
     1981 gcaacagacc catgtcgcgc aactgcacca tgtggacaca caacaggtcg tcccagttgc
     2041 ccgactcggg cggcatgacc tcgtaggtgt acgaatcgat gaacgcgccg gcgacgtcgt
     2101 gatcggccat ccactcgaca caactgaacc cgaagccgac cgttcgccag tccttgccca
     2161 cggcgtagcg gcgtcggtcc ccctcgaaat accacttcag gtcgccggtg cgcacacaga
     2221 tggcgtcgcc cggttcgatg ctcagccccg tcgcctcgag cgctcggtcg agatcttccg
     2281 cggtcaccgc gtggccatga tcgacccgat ccgagccgtg gagcttcgcg acgtccaaca
     2341 ggatgcctcg cgtcacgacc gacggcagct tctcggcccc gcaccacgtc gccccgttgt
     2401 gagcaccgat cgaacgcttg ggccggttgt tgtagaggag gtcgtcgtag gagatatggc
     2461 tgagggcatc gatgtgggtg gccgcacacg tcgacatcat cacgagatcg tcgcatccct
     2521 cccaaatgcc cggcgccatc ttgtcgcgct cgttcatcga ggtgaacgtg aggatcgggt
     2581 tgtagcggcc ggccggctgg ccaacctgga tgccgtcgtt tcgcaggtcg accgcgagac
     2641 tgaaccgctt accggtccgc acgcacgccg ccccgcgctg ggcggcctcg ggggtcagga
     2701 ggttgccaca cccgagttcg tcgtcatcac cccaacgccc ccagttggag accttggccg
     2761 cggtggcgag cacgtgttct gagatcgcca tgaccactca gcccatcgtc agcacgccac
     2821 gggccaactt gccctcgtgc aactcgtcga gcgccttttg gaagtcctcg atcggatagg
     2881 tggcactcac gagttcgtcg agcttgagac gaccggcctc gtagagccgc accatgagcg
     2941 gaatgtcgta ttggggtcga gccgaaccgt agcgacaccc catgatcgtc ttgtcgttgt
     3001 acatggtgtt gacgacgaag ctcgcctcgg agcccatctt cggcacgccc aacatgacga
     3061 gcttgccacc ccaatcgagc agttcggtcg aggtcttgat gagcgccgtc gacccgacgc
     3121 actcgaagac atggtcgaca ccgttcgggc agatttcttt caccgcggcg accaggtcga
     3181 agtctggtcc gtcggggcag atgaaatgcg tcgcgccgaa ctgcctggca aacgcttcct
     3241 tgcccgggtt ggtgtcgacc gcgatcaccg ggagcgcacc gctgagggcg gcaccctgca
     3301 gggcgttgag cccgatgccg ccgacgccga tcacgaccgc cgactcgccc tgagcgacct
     3361 gcgctcggtt gaacgctgcg ccgacgccgg tgagcacgcc gcagccgatg agacacgctg
     3421 tggtgagcgg tacgtcgttg gggatgcgca cggcctgggt tgccttgatg acggtctctt
     3481 cggcgaacac cccgaggttg gcgaactgga agcccttctt ggcgtcctcg ccctcaccga
     3541 tgagataggg gcggttgagc ttgccgaggg ttgcccgaca aaacgtcggc tttccacggt
     3601 cgcactgggc gcactggccg cagtttccga gggtcgacat gacgacgtgg tcgccgaccg
     3661 caacgttggt cacatcgggg gcgacctcga tgacctcgcc tgcgccctcg tggcccaaca
     3721 cgaccggcgg agggaactga atcgtcccgt tcaacaccga cacgtcgcta tggcagagcc
     3781 ctgcggcggc gatgcgcacc cgcacctccc ccggacgcac accgcggatg gtgacgtcgt
     3841 tgcgaactgt tgcggtcttg ccgtcgaaga cgagcgcttt catcagaccc ccaaagagct
     3901 gtttgtggag aagaccatgt tacgacaccg cctagaacgc gtttcagttc accggctgtg
     3961 gcaccatctc gaaaccgacc agagcgccga cgtcgtcgac ccacgtgatg agcaaccacg
     4021 cgtcctcacc ggggttccgc ccgtcgggga ggactccggt ccaccttgcg ccgtcagcct
     4081 cagaggcctc cggatcggcg gtcagcgaag cgaccgaacg cgactgggca aaccacacga
     4141 gcatctcacc aggggcaacc tcgaccccct ggatggcgga gaccgggggc agtacggaaa
     4201 tcgtggacct cgagacggtg gaggtgatct cggcggccca gggcatggag ccatcgccga
     4261 acgcagtgcg ccccgccgat atcccgccaa agcgccagtc accgtcctcg ccgtcgtaga
     4321 cctgcatcca cccgtgatgc tgcgcgccgg cgagtcgaat gccgacgccg cagctaccac
     4381 cgccgaagct gatcgttccc ggttccgcca ggaacccgcg gccgatgacc ggggcgatcc
     4441 gtgcgttcgc gaccgctgct accgcctctt cgatgcccgg ttcggccgaa acggcccacg
     4501 tcggggtctc gccctccccc cgatgcgggc cgcattccca gttgcccgac ccgtccgagc
     4561 catagctcgt gatctcgggc gacccgtcac gcaccgcaac ggtatcgacc tccctggtcg
     4621 cctcccacca gaacgcagtt cccgccacaa cgagcccaac cgaacacagc acgaagagaa
     4681 ccttggggcg acg
//
LOCUS       DAOOMA010000142         4690 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_22007_length_4690_cov_3.732039, whole genome shotgun sequence.
ACCESSION   DAOOMA010000142 DAOOMA010000000
VERSION     DAOOMA010000142.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 4690)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4690)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4690
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22007_length_4690_cov_3.732039"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1729
                     /locus_tag="PLP95_05155"
     CDS             <1..1729
                     /locus_tag="PLP95_05155"
                     /inference="COORDINATES: protein motif:HMM:NF012905.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cation-transporting P-type ATPase"
                     /protein_id="HPB45225.1"
                     /translation="VETLGSTSVICSDKTGTLTMNRMSAVELQSAGQRHEITTDGDSV
                     DIDAASFDEPRIRDLLELAVLSSDAIVTLGEGARDVGDPTEVGIVRLAAQVGIDAETL
                     RRDRPRVAEVPFDSGRKYMVAVIDEGDPDNVRFAIKGAIDRLIGRCSAMRDGDRTVPL
                     GEEQRDLVSAAVDAAARQGRRVLAVCTTTAPRSVIAGANQERLVELSRELELVGLVGL
                     ADPPRPEAADAIARAQRAGISLKMITGDHPTTATAIAQEIGIVGDTVSGTELDGWSDE
                     HLTERVEDIGVFARVSPEHKLRIVRSLQARGNVIAMTGDGVNDAPSLKQADVGVAMGI
                     TGTEVSKEAADVVLADDQLGSIVAAVGQGRAIYDNIVTFVRFQVATNIGAILTLVGSQ
                     LIGLPKPFAAIQLLWINIIMDGPPAMALGTDKPKPGVMRRKPRRPDEIILNRHRLGVI
                     GLHGAIMAAVTLSILSYGEREWGEAVGATMAFTVFVLFQIVNAFNVRAVGTLFQPHTF
                     TNKILWGALALVTVLQIMVIEAPFMNNLFETTNLTLGQWGICVAAALTLGVVDEVRRI
                     ITFKRGQSD"
     gene            complement(1787..3217)
                     /locus_tag="PLP95_05160"
     CDS             complement(1787..3217)
                     /locus_tag="PLP95_05160"
                     /EC_number="1.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015798022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminobutyraldehyde dehydrogenase"
                     /protein_id="HPB45226.1"
                     /translation="MTDTIRNFIGGQQVDAASGETTDLVDPCTGDVFASAVRSGVDDV
                     DRAARAAADAFAQWGRTTPSERQAALLKIADAIESRAAEFVAAESRNTGKPVELTMSE
                     EIGPMVDQIRFFAGAARTGAVPATGEYLSGHTSSIRREPIGVVAQVAPWNYPMMMAVW
                     KWAPALAAGNTVVLKPSDTTPVTTVMMAELMNEFLPDGVFNVICGDRDTGRALVAHEI
                     PQLVSITGSVRAGMEVAQQAAATLKRCHLELGGKAPVVVFGDADLDAAAEAIAVAGYF
                     NAGQDCTAATRVLVADGAYDDFVERLTEQARNTSTGFPDDPDVLYGPLNNAAQFARVT
                     GFFERVPDHAQVRIGAERVGESGFFVAPTVISDLRQTDELIQNEIFGPVITVQRFRDE
                     PEAVAYANGTPYGLASSVWTSDHGTAMRMSRALDFGCVWINTHIPLVAEMPHGGFKMS
                     GYGKDLSAYALDDYTRIKHVMSNIDA"
     gene            3405..>4690
                     /locus_tag="PLP95_05165"
     CDS             3405..>4690
                     /locus_tag="PLP95_05165"
                     /inference="COORDINATES: protein motif:HMM:NF012428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HPB45227.1"
                     /translation="MPTPSNPVPIERLTANAKAIAETEMATVLANTTASKVLYERAVR
                     SMPGGVASSFQLGDPYPIYLSRGVGANVWDVDGTEYVDFHNGFGSIAVGHAHPAVADA
                     VCTAARSGMHFAVTVEKTVALAEELCRRFGVDQVRFTNSGTESNMSAIRLARAATGRE
                     VIAKIEGSYHGHVDQLMYSCTPGAESMGGRDTPAKTPKSKGMPKDLDQWIRIVPFNDA
                     DAFERLLEAEGDQIAALIMEPVMMNIGIVVPEPGYLERIRELCTRHGVVLIFDEVKCG
                     VTIAAGGAAERFGVKPDLASWAKSIGGGAAIGAFGGRADLMDHINHGAAHQGTYNGNP
                     LSVAASLATLTEVLTDDAYVHFAALGERLTAGMNEAIAAYGIPAHTVDLGAKGCVTYR
                     SQPLTNYRDFLDTNMDLYLASYPWMINRGVFMTPGD"
BASE COUNT          843 a         1600 c         1475 g          772 t
ORIGIN      
        1 cgtggaaacc ctcgggtcga cctcggtgat ctgctcggac aagaccggca ccttgacgat
       61 gaatcgcatg tcggcggttg aattgcagtc ggccgggcaa cgccacgaga tcacgaccga
      121 cggcgattcg gtcgatatcg acgcagcctc gttcgacgag ccgcgcatac gcgacctcct
      181 cgagttggcg gtgttgtcct cagacgccat cgtcacgctc ggcgagggcg cccgagatgt
      241 cggcgacccg accgaggtcg gcatcgtgcg gctcgccgcc caggtcggca tcgacgctga
      301 gacgcttcgc cgagaccgcc ctcgcgttgc cgaagtcccg ttcgactccg gtcgaaagta
      361 catggtcgcg gtcatcgacg aaggagaccc cgacaacgtt cgcttcgcga tcaagggcgc
      421 catcgaccgc ctgatcggcc gatgttccgc gatgcgcgac ggcgaccgca ccgttcccct
      481 cggtgaggaa caacgcgatc tcgtctcggc agcggtcgac gccgcagcgc gccaaggccg
      541 ccgggtgctc gccgtgtgca ccaccaccgc gccgcggtcg gtcatcgccg gcgcgaacca
      601 ggagcgactc gtcgaactgt cccgcgagct cgaactcgtc ggcctcgtcg gcctcgcaga
      661 tcccccgcga ccagaggccg ccgacgcaat cgcccgcgcc caacgcgccg gaatctcact
      721 gaagatgatc accggcgacc accccacgac cgcgaccgcc atcgcccagg agatcggcat
      781 cgtcggcgac acggtctcgg ggaccgaact cgacggctgg agcgacgagc atctcaccga
      841 acgggtcgag gacatcggcg tgttcgcacg ggtctcgccc gaacacaagt tgcgtatcgt
      901 gcgctcactg caggcacggg gcaacgtcat cgcgatgacc ggcgacgggg tcaacgacgc
      961 accctcgctg aagcaggccg atgtcggcgt ggccatgggc atcaccggca ccgaagtctc
     1021 caaggaggcg gccgacgtcg tcctcgccga tgaccaactc ggcagcatcg tcgccgcggt
     1081 cggtcagggc cgggcgatct acgacaacat cgtcaccttc gtgcgcttcc aggtggcgac
     1141 caacatcggg gcgatcctca ccctcgtcgg atcacaactc atcggcttgc ccaaaccctt
     1201 cgccgccatc caactgctgt ggatcaacat catcatggac ggccccccgg cgatggcgct
     1261 cggcaccgac aagcccaagc cgggcgtcat gcggcgcaag ccgcgccgac ccgacgagat
     1321 catcttgaat cgccaccgac tcggcgtcat cggcctccat ggcgccatca tggcggcggt
     1381 cacgctgtcg attctcagct acggcgaacg cgaatggggc gaggccgtcg gcgccacgat
     1441 ggccttcacg gtgttcgtgt tgttccagat cgtcaatgcg ttcaacgtgc gagccgtcgg
     1501 cacgctcttt cagccacaca cgttcaccaa caagatcctg tggggggcgc tcgcactggt
     1561 cacggtgttg cagatcatgg tgatcgaggc gccgtttatg aacaacctct tcgagacgac
     1621 caatctgacg ctcggccaat ggggcatctg cgtcgcggcc gcgctcacgc tcggtgtcgt
     1681 cgacgaggtg cgtcgcatca tcacgttcaa acgcggccaa agcgactgat cggccacacc
     1741 ggtggatctt gggtgccccg ccgccggggc acccaagatc gcctcgtcag gcgtcgatgt
     1801 tgctcataac gtgcttgata cgggtgtaat cgtcgagagc gtaggccgac aggtccttgc
     1861 cgtagcccga catcttgaac cctccgtggg gcatctcggc cacgagcgga atgtgggtgt
     1921 tgatccacac gcaaccgaag tcaagagcgc gactcatgcg catcgcggtt ccgtgatcgc
     1981 tggtccacac cgaactcgcc agcccgtacg gcgtgccgtt cgcataggcg acggcctcgg
     2041 gctcgtcgcg aaaccgctgc accgtgatga ccggaccgaa gatctcgttc tgaatgagct
     2101 cgtcggtctg gcgcaggtca ctgatgaccg tgggggccac gaagaatccc gactcgccca
     2161 cgcgttcggc gccgatgcgc acctgcgcgt ggtcgggaac ccgctcgaag aagccggtga
     2221 cccgggcgaa ctgagcggcg ttgttcaacg ggccgtacaa cacgtcgggg tcatcgggaa
     2281 atccggtgga ggtgttgcgc gcctgttcgg tgagccgttc gacgaaatcg tcgtaggccc
     2341 catcggcgac gagaacacgg gtggccgccg tgcagtcctg accggcgttg aagtagcccg
     2401 ccaccgcgat ggcctcggcc gccgcatcca agtcggcatc gccgaacacc acgaccggcg
     2461 cctttccacc gagttcgagg tggcatcgct tcaacgtcgc cgccgcctgt tgtgccacct
     2521 ccattccggc tcgcaccgat cctgtgatgg acacgagttg tgggatctcg tgtgcgacca
     2581 gcgcccggcc ggtatcgcgg tcgccgcaga tgacgttgaa caccccatcc gggagaaact
     2641 cgttcattag ttccgccatc atcaccgtgg tgaccggggt cgtgtcggag ggcttgagca
     2701 ccacggtgtt gccggcggcg agcgcagggg cccacttcca caccgccatc atcatcggat
     2761 aattccacgg cgcgacctgg gccacgacgc cgatcggctc gcgtcggatg gaactggtgt
     2821 gacccgacag gtactcgccc gtcgccggca cggcaccggt acgggcggcg cctgcaaaga
     2881 agcggatctg gtcgaccatc gggccgatct cctcgctcat cgtgagctcg accggcttgc
     2941 cggtattgcg cgactccgca gcgacgaact ccgcggcgcg ggattcgatc gcgtcggcga
     3001 tcttcaacag cgcggcctgg cgctcgctcg gggtggtgcg cccccactgt gcgaacgcat
     3061 cggcggccgc ccgcgctgcc cggtcgacgt cgtccacacc cgaacgaacc gccgacgcga
     3121 agacgtctcc ggtacatgga tcgacgagat cggtcgtctc ccccgacgca gcgtcaacct
     3181 gttgtccacc gatgaagttg cgaatcgtgt ccgtcatgag cgaagccttg cccatcgcca
     3241 cgcggtttgc aacgagtcgc ctcacaccgc cgatgttgcc ccatcgtccc cattggtcag
     3301 gttgataggg ttgccgagca accggtgagc cgggcgagtc gacgcggcta attgatgccc
     3361 aaccgccgac accactcgcc acggcccaat ctgagggagc gaacatgcca acaccgtcca
     3421 accccgtccc gatcgagcgg ttgaccgcga acgccaaggc gatcgcggag actgaaatgg
     3481 caacggtgct cgccaacacg accgcgtcaa aggtgctgta tgagcgggcg gtgcgctcga
     3541 tgcccggcgg cgtcgcgtcg agcttccaac tcggcgaccc gtaccccatc tatctgagcc
     3601 gcggcgtcgg cgcgaacgtg tgggacgtcg acggaacgga atacgtcgac tttcacaacg
     3661 gattcggatc catcgccgtc gggcatgctc atccggcggt tgccgatgct gtctgcactg
     3721 cggcgcgcag cggcatgcac ttcgcggtga cggtcgaaaa gacggtggcg ttggcggagg
     3781 aactgtgtcg acgtttcggc gtcgatcagg tccggttcac caattccggc accgagtcga
     3841 acatgagcgc catccgcctc gcccgcgccg cgaccggacg agaggtcatc gccaagatcg
     3901 aaggctccta tcacggccac gtcgatcaac tgatgtacag ctgcacgcct ggggccgaat
     3961 cgatgggcgg gcgcgacacc ccggccaaga ccccgaagtc caagggcatg cccaaggacc
     4021 tcgaccagtg gattcgcatc gtgccgttca acgacgctga tgccttcgaa cgactgctcg
     4081 aggccgaggg ggatcagatc gccgccctca tcatggaacc ggtgatgatg aacatcggca
     4141 tcgtggtgcc cgaaccgggc tacctcgaac gcatccgcga gctgtgcacc cgccacggcg
     4201 tggtgctgat tttcgatgag gtgaagtgcg gggtcacgat cgccgctggc ggagccgcgg
     4261 aacgcttcgg cgtgaagccg gacctggcga gctgggcgaa atccatcggg ggtggcgcag
     4321 ccatcggtgc cttcggcggc agagccgacc tcatggatca catcaaccac ggggctgctc
     4381 accaaggcac ctataacggc aacccgttgt cggtcgcggc gtcgctcgcc acgctcaccg
     4441 aagtcctcac cgacgatgcg tacgtacact tcgcggcgct cggtgagcgg ctcaccgccg
     4501 gcatgaacga ggcgatcgcg gcctacggca tcccggccca caccgtcgac ctcggcgcca
     4561 agggctgtgt gacgtaccga tctcagccgc tcaccaacta ccgcgacttc ctcgacacca
     4621 atatggatct gtacctggcg tcgtaccctt ggatgatcaa ccgcggcgtg ttcatgactc
     4681 cgggcgacga
//
LOCUS       DAOOMA010000143         4675 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_22095_length_4675_cov_4.114935, whole genome shotgun sequence.
ACCESSION   DAOOMA010000143 DAOOMA010000000
VERSION     DAOOMA010000143.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 4675)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4675)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4675
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22095_length_4675_cov_4.114935"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..509)
                     /locus_tag="PLP95_05170"
     CDS             complement(<1..509)
                     /locus_tag="PLP95_05170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB45228.1"
                     /translation="MRRRTFLASGFAAVAAPSLMACSTSDPTEPPGSGASTTGSAATT
                     GSGTGPGGFAARAARYLSVAAQHEPASDASRARVWRATAQAVLGQTPELSVADLETIT
                     ERFARFEDTTDFDMVALINLWYRSDFGAMVPEETAAHLRRLILEFKYWYTEPQPEGIT
                     DERWYWSEN"
     gene            757..1785
                     /locus_tag="PLP95_05175"
     CDS             757..1785
                     /locus_tag="PLP95_05175"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HPB45229.1"
                     /translation="MASLRLGVIGIEHLHLFEMVDQLAATGFETVCHVGQGSLEELYR
                     NWRGNSELRTPADIIGDDSLDLVVLAGVPSTRAATAVAAMHAGRHVLVAKPAVTSADD
                     LALIDRTARSTGRRWWVFFSERLSNRAITKAVELVGAGAIGDLVSISGWAPHSLQPTS
                     RPDWFFNPMLSGGILVDLAAHQGDQLLALAGPGITEVASATVRNAASPTCPQFQDIGR
                     MSLRHTTASGRVVLSDHHVDWLSPSGLGTWGDVRLNLVGAAGTIEVRSNIDVTGIDGS
                     EHLILVDGDGARRIDCTATDVDWAARLSADIASGSDSLMSHEHAVSACELTLRAQAVA
                     DGSYSSGG"
     gene            1787..2818
                     /locus_tag="PLP95_05180"
     CDS             1787..2818
                     /locus_tag="PLP95_05180"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HPB45230.1"
                     /translation="MSPNRLRVGVVGLGIGQAHLLAYVLDPDRFEVVAIAEPDRERRD
                     RGAADWGVPAVPSLDDLLNFELDVVDLCTPPALHEQQCLTVLESGRHVICEKPLVATL
                     GALERLEAAEVAATGRLMPIFQYRFGRGISQAKAVIDAGLGGPLRLATSETSWCRGSD
                     YYSAAWRGTWSGEGGGAVFGHGTHAHDLISYLAGPLTSVTGQVATLVNDIETEDTATG
                     FGRTTDGGLVSMAVTLGSVHEITRLRFSFQHVSIESSTAPYEPGDGSWRFDWDDDALA
                     DEAEGIWAAMRETPSGFGGQLEAFHRAVTTGADLPVTLADARRALELVMGWYESARTG
                     SAVPLPSSR"
     gene            2923..4023
                     /locus_tag="PLP95_05185"
     CDS             2923..4023
                     /locus_tag="PLP95_05185"
                     /inference="COORDINATES: protein motif:HMM:NF024589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPB45231.1"
                     /translation="MDPRSNPYNPGAGLRPFALTGRDDDIEAVEVLADRATRGLMSRS
                     MVFSGLRGVGKTVLLNELAGRTQQRNWFTVQIEADRSHPERFARSLAVEFINGARSQR
                     RWFSKRNEQVRDALRTITSFQISLGLDGFRFDIDLDRSSARANTGDLYIDLADLATSV
                     GVAASESGIGVALFIDEMQDLTAEQMSAVCRSCHLAAQRHLPWFVIGGGLPNLPKALA
                     EAESYAERLFEYRTIDRLGDEAARSALVLPTAAADVVWANDACEFALSESGGYPYFIQ
                     QFDHGFYASRWERATPTERRFLEAMAPDDGASSASSDVAGRLGKSLSSLGPARASLIN
                     KGIVYSPEHGSIAFTVPGMADFVRRRARAERP"
     gene            4081..>4675
                     /locus_tag="PLP95_05190"
     CDS             4081..>4675
                     /locus_tag="PLP95_05190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HPB45232.1"
                     /translation="MSAPALLTERDGHALVITLNRPERQNAINGEMLVRLYDALVEAE
                     QDPEIRVAILTGAGGNFCSGMDLKEMAGGFNGDSGEIDVPARLGADPDLHWKALLRSW
                     KPAVPLIIAVEGTAIAGGTELLGATEIRVAGESARLGIAEVRRALYPMGGSAVRIPRQ
                     IPWARAAELLLCGNTITAQEGYDLGLINHVVPDGEALT"
BASE COUNT          761 a         1607 c         1501 g          806 t
ORIGIN      
        1 tggttctccg accagtacca gcgctcgtcg gtgatcccct cgggttgggg ttcggtgtac
       61 cagtacttga actcgaggat cagtcgacgc aggtgcgcgg ctgtctcctc cgggaccatg
      121 gcgccgaagt cgctgcgata ccagaggttg atgagcgcca ccatgtcgaa gtcggtggtg
      181 tcctcgaacc gagcgaaccg ctcggtgatc gtctccaggt cggccaccga cagttccggc
      241 gtttgtccaa gcaccgcctg agccgtcgca cgccataccc gggctcgact ggcgtcggat
      301 gcgggctcgt gctgcgccgc aacgctcaag tagcgggccg cccgcgccgc gaacccgccc
      361 ggcccggtgc cgcttccggt ggtcgccgcg gagccggtcg tcgaggcgcc cgatccgggc
      421 ggttctgtgg gatccgaggt cgagcaggcc atcagcgagg gcgcggcgac ggccgcgaag
      481 ccggaggcga gaaacgtgcg tcggcgcatc gcagcaacct accgaactcg ccccgtgcgc
      541 gcctcggctc gggaccacgc ccccgactgt cggcaggcgc gaacctgtgg tgattcggcg
      601 ccggagaact cacggcctca ccggcgcagc gatcgagtca cgccggcacc ccctacactc
      661 tgcgcatggt cgagggcacc agccaacgga tcctcccggc gggggcgcgg gccgtcgaac
      721 ggcactcagc gtcgacccgg gctaccggat agcgccatgg cctcgctgcg actgggcgtg
      781 atcggtatcg agcatctcca tctcttcgag atggtcgacc agctcgccgc cacaggcttc
      841 gagacggtgt gccacgttgg ccagggatcg ctcgaggagc tgtaccgaaa ctggcgcggt
      901 aactcagagc tgcgaacccc cgccgacatc atcggcgacg acagcctcga cctcgtcgtc
      961 ctcgccggcg tgccgtcgac gcgggccgcc accgccgtcg ccgcgatgca cgccggtcgt
     1021 cacgtgctcg tcgccaaacc ggcggtgacc tcggccgacg acctggcgct catcgatcgc
     1081 acggcacggt cgaccgggcg gagatggtgg gtgttcttct ccgaacgcct gagcaaccgg
     1141 gcgatcacca aggccgtcga gttggtcggc gcgggagcca tcggagacct ggtctcgatc
     1201 agtggatggg cgcctcattc gctgcaaccc acgagtcggc ccgactggtt cttcaacccg
     1261 atgctctccg gcgggattct ggtggatctt gccgcccatc aaggcgatca actcctcgca
     1321 ctcgccggcc ccggaatcac cgaggttgcc agcgccaccg tccgcaacgc cgcgtcgccc
     1381 acctgcccgc agtttcaaga catcggacgg atgtcgcttc gacacaccac ggcgagtggc
     1441 cgggtggtgc tgagcgatca tcacgtcgac tggctctcac cgagcggtct cggcacctgg
     1501 ggcgatgttc ggctcaacct cgtgggtgcg gcgggcacca tcgaggttcg gtcgaacatc
     1561 gacgtcaccg ggatcgacgg ttccgagcat ctgatcctcg tcgatggcga cggcgctcga
     1621 cgcatcgact gcaccgccac cgatgtcgac tgggcagcgc ggctctcggc cgacatcgcc
     1681 tccggcagcg attcgttgat gagccacgaa cacgccgtgt ccgcgtgcga gttgaccctg
     1741 cgagcacagg cggtcgccga cggctcgtac tcctccggag gttgagatga gtccgaaccg
     1801 tttgcgagtt ggcgtggtcg gccttgggat cggccaggct cacctgctgg cctatgtgct
     1861 ggatcccgac cgtttcgagg tcgtcgcgat cgccgaaccg gaccgggagc gccgcgaccg
     1921 cggtgcagcc gactggggtg tgcccgccgt gccatcgctc gatgacctgt tgaatttcga
     1981 actcgacgtg gtcgatctgt gcacgccccc ggcgctgcac gaacaacagt gcctcaccgt
     2041 ccttgaatcc ggacgccacg tcatctgcga aaagccgctg gtggcaaccc tcggcgcgct
     2101 cgagcgtctc gaggccgccg aggttgcggc gaccggacgg ctcatgccga tcttccagta
     2161 ccggttcggg cgcgggattt cacaggccaa ggcggtcatc gacgccgggc tcggaggccc
     2221 gcttcggctc gcgacctccg agacctcatg gtgtcgcggt tcggactatt actccgctgc
     2281 ctggcggggc acgtggtcag gcgagggtgg cggcgcggtg ttcgggcatg gaacccacgc
     2341 gcacgatttg atcagctacc tcgccgggcc gctgacctcg gtgaccggac aggtggcgac
     2401 gctcgtcaac gacatcgaga ccgaggacac cgcgaccgga ttcgggcgca ccacagacgg
     2461 cggcctcgtc tccatggccg tgaccttggg gtcggtgcac gagatcacgc gactccgctt
     2521 cagtttccag cacgtcagca tcgagtcctc caccgctccc tacgaacccg gcgacggatc
     2581 atggcgattc gattgggacg atgacgcgct cgccgacgag gccgaaggga tctgggccgc
     2641 gatgcgcgag accccatcgg ggttcggcgg tcagctcgaa gcgtttcacc gtgccgtcac
     2701 caccggtgcc gacctgccgg tgaccctcgc ggatgcccga cgagcgctcg aactggttat
     2761 gggttggtac gagtcggccc gcaccggatc agcggtgccg ttgccctctt cgcggtgact
     2821 tcggtcgcgt ccgatcacga gtgatgcagc ccaatctatc gatctgtagg catttctagc
     2881 atttttccta tacttgccta gatatcgcta tatcctcgag tgatggaccc tcgttccaat
     2941 ccgtacaacc ctggggccgg gcttcgaccc ttcgccctca ccggacgaga cgacgacatc
     3001 gaggcggtcg aggtactcgc cgaccgcgcg actcgcgggc tgatgtcgcg cagcatggtc
     3061 ttcagcgggc ttcgcggcgt gggcaagacc gtgctgttga acgaactggc gggacgaacc
     3121 caacagcgca attggttcac cgtgcagatc gaggccgatc gttcccaccc cgagcgtttc
     3181 gcccgttcgt tggccgtcga attcatcaac ggagcccgca gtcagcgccg ctggttctcc
     3241 aagcggaacg aacaggtccg agacgcactc cgaacgatca cctcatttca gatatcgctt
     3301 ggcctcgacg gattccggtt cgacatcgac ctcgaccggt cctccgcccg ggcgaacacc
     3361 ggcgacctct acatcgacct ggccgacctg gccacgtccg tcggagtcgc cgcatctgaa
     3421 tcgggaattg gggtggcgct gttcatcgac gagatgcagg acctcacagc ggaacagatg
     3481 tcggcggtat gccgatcctg ccacctcgcc gcgcaacgcc acctgccctg gttcgtgatc
     3541 ggtggcggtc ttcccaacct ccccaaggcg ctcgccgaag ccgagagtta cgccgagcgg
     3601 cttttcgagt atcggaccat cgaccggttg ggcgacgagg cggcgcgttc ggcgctggtc
     3661 ttgccgactg cagcggccga tgtcgtttgg gccaatgacg cctgcgaatt cgcgttgagc
     3721 gaatcgggcg ggtaccccta tttcattcag cagttcgatc acggcttcta cgcgtcccgt
     3781 tgggagcgag cgacgccgac cgaacgacgc tttctcgaag cgatggcccc cgacgacgga
     3841 gcatcgtcgg ccagcagcga cgtcgccggc cggctcggca agtcgctgag ctccctcggc
     3901 cccgcgcggg cgagtctcat caacaagggc atcgtctatt cgcccgaaca cggctccatc
     3961 gccttcaccg ttccaggcat ggccgacttc gttcgccgcc gcgccagggc cgaacggcca
     4021 tgaacgcggt cgctgacagc ccgatagaac gcgttctcgt tttcttctaa cctgcgaacc
     4081 atgtctgcac ccgcactgct caccgagcgc gacggtcacg cgctcgtcat caccctgaat
     4141 cgccccgaac gacaaaacgc catcaatggc gagatgctcg tgcggctcta cgacgccctt
     4201 gtcgaggcgg aacaggatcc cgagatccgc gtcgccatcc tcaccggcgc cggcggcaac
     4261 ttctgttcgg gaatggacct caaggagatg gccgggggct tcaacggtga ctccggtgag
     4321 atcgacgtcc cggctcgcct cggcgcagac cccgacctgc actggaaggc gctgcttcgg
     4381 tcgtggaagc cggcggtgcc gctcatcatc gcagtcgaag gaaccgccat tgccggtggt
     4441 accgaactgc tcggcgccac cgagattcgc gtcgccggcg aatccgcgcg actgggcatt
     4501 gccgaggttc gccgggcgct gtaccccatg ggcggatcag ccgtgcgcat tccacgccag
     4561 atcccgtggg cccgtgccgc ggaactgctg ttgtgcggca acacgatcac tgcgcaggag
     4621 ggctacgacc tcggcctcat caaccacgtg gtacccgacg gcgaggcgct gacca
//
LOCUS       DAOOMA010000144         4675 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_22098_length_4675_cov_3.756494, whole genome shotgun sequence.
ACCESSION   DAOOMA010000144 DAOOMA010000000
VERSION     DAOOMA010000144.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 4675)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4675)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4675
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22098_length_4675_cov_3.756494"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            59..1834
                     /locus_tag="PLP95_05195"
     CDS             59..1834
                     /locus_tag="PLP95_05195"
                     /inference="COORDINATES: protein motif:HMM:NF016086.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="circularly permuted type 2 ATP-grasp protein"
                     /protein_id="HPB45233.1"
                     /translation="MAQIGDRAERVHVIMRLVSSERCDPLELATSTEAGVPGLVEACR
                     RGTVTLANPLGSGIGESTALLHFLPELTRTLLGEDPLIEAAPTWWCANDQHRRHVLAN
                     LDRLLIRSSGGGSGRRTLFGRMLSSDERALLASEIEAAPHRFVGQQEVEFEPQAVFDG
                     DRLTHAPVVTRAYLVAGNSGFEAMPGGLSITATTTDAITFDAPVRVKDTWVYEAEGVA
                     SDMNPRQVVVAATRQPPVDLRDSITSRAAASMYWIGRNLERALTTVRLVRSVALLHES
                     RHDLWSDTSPMVDAGIDLLIDSIVGAAPNPSFVGDGRGRLTNEVLPAALTDSGRPRSL
                     ASSLYYLLSNVESVRQLFSADSWRALQRITERYRSLLNDGNVSTTQDALDTFSPLLEL
                     SALIAESMVRDPGWWFLDLGRRVEGAQLATGSIGATLQRDPPEEIAASVWNALLGTWD
                     GVFAYRRRYRSDVDPSLLIDLLLCDTSNPHSVCGNLAGLTRALDHLHSLRGAGPNRSP
                     AAQQCAALLESLQGANVEALAAVGADRTRRSLSEFTTGLTTAIDAVAEDIDLTYFVQL
                     SSRHLEGSRLPTEAAGVASVGQASA"
     gene            1831..2724
                     /locus_tag="PLP95_05200"
     CDS             1831..2724
                     /locus_tag="PLP95_05200"
                     /inference="COORDINATES: protein motif:HMM:NF013961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase family protein"
                     /protein_id="HPB45234.1"
                     /translation="MRFRVSHTTAYRSRLPVVMGYHLAYLTPRDCPGQRVRSHELLVT
                     PTPADRAGHVDVFGNRFTYFCIEEPHVLFEVTATSEVEVSGRRRFADPQADWHSGVER
                     ATDPLLADFVAFSDFVTSSPSVRDYAALSFPPGRPLLEALEELNRRIFDDFAYDPGWT
                     TVSTPVEVVFATRRGVCQDFAHLMLACVRSMGLAGRYVSGYLENRPPDGTDALAGADA
                     SHAWVSVLSGDGIWIDLDPTNGLVEPAHHITVAWGREYSDVAPLRGVVTAPGDPATLS
                     VAVEVERIDYRSPRRHHRNPR"
     gene            2755..3876
                     /locus_tag="PLP95_05205"
     CDS             2755..3876
                     /locus_tag="PLP95_05205"
                     /inference="COORDINATES: protein motif:HMM:NF013775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HPB45235.1"
                     /translation="MNQNDTPRVTKSSRDHGELRRRFESWLREQLGHDANPVITSFEA
                     PESNGMSSETILVSAVWDSPDGRGDHDLVVRIAPPSDSMPVFSRYDLSLQYDVMALVG
                     ERTDVPIPRLLWNEPSPEPLGAPFFVMGRSIGDVPPDLMPYTFGSWVSEATDSDRERM
                     ERTTVDVLTKVAAIADPQVSVPSLAPEPDDGRSALRCHVDAARAWLDWSVGDEASPLL
                     DRCFEWLESNWPSDEGETVLSWGDARIGNIMYRDFEPVAVLDWEMAALGPRELDLAWL
                     IFLHRFFDDIAVQMGLQAMPDFLRRDRMESLYEELSGHCPQNMDWYLMYSAVRHGIVF
                     TRISRRMAHFGDQEMPEDPDDAILHRAALEAMVEGTYWS"
     gene            3952..4036
                     /locus_tag="PLP95_05210"
     tRNA            3952..4036
                     /locus_tag="PLP95_05210"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3985..3987,aa:Leu,seq:taa)
     gene            complement(4160..>4675)
                     /locus_tag="PLP95_05215"
     CDS             complement(4160..>4675)
                     /locus_tag="PLP95_05215"
                     /inference="COORDINATES: protein motif:HMM:NF019371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Abi family protein"
                     /protein_id="HPB45236.1"
                     /translation="EYGKRYPLWVLADHLDFADSSRLYEGLPARLQWAVAERIGVRID
                     LSKLSKRQQEKAKKHHPLVRWLEQLTIVRNTSAHHSRPWNRSFTPASTAGLASIPGLS
                     TLPDGQSERIYGAALVTAQILTTVSPGSSWAPKLRSLILESLEAIPRRRAGEMGCPAG
                     WDSAVESPLAK"
BASE COUNT          776 a         1582 c         1493 g          824 t
ORIGIN      
        1 gacgcctcgg gttcaccctc gtcgaaccgc ccgacctcac cgtgcgcgac ggacgcgtgt
       61 ggctcaaatc ggtgaccggg ctgagcgggt tcatgtcatc atgcggctcg tctcctcgga
      121 gcgttgcgat ccgctcgagc tcgccacgag caccgaggca ggggtgcccg ggctggtcga
      181 ggcctgtcgc cgcggcacgg tcacactcgc caacccactc gggtcgggaa tcggagagtc
      241 gaccgccctg ctgcacttcc tccccgaact cacccggacc ctgctgggag aagacccgtt
      301 gatcgaggcc gccccgacct ggtggtgcgc caacgaccag caccgacgcc acgtcctggc
      361 caacctcgac cgattgctga tccgctccag cggtggcggc tccggtcggc ggaccctgtt
      421 cggccgcatg ctgtcgagcg acgagcgggc gctgctcgcg agcgagatcg aggccgctcc
      481 acatcgcttc gtcggccaac aagaagtcga gttcgagcca caggccgttt tcgacggaga
      541 ccggctgacg catgctccgg tggtcacgcg ggcctacctg gtcgccggca attccggatt
      601 cgaagccatg ccgggcgggt tgtcgatcac cgccacgacc accgacgcca tcaccttcga
      661 tgctcccgtc agggtgaaag acacctgggt ctatgaggcc gaaggcgtcg cgtccgacat
      721 gaatccccgc caggtcgtcg tcgccgccac ccgtcagcct cccgtcgatc tgcgcgactc
      781 gatcaccagc cgggccgcag cttccatgta ctggatcggc cgcaacctcg agcgtgccct
      841 caccacggtg cgccttgtcc gctcggtcgc attgctccac gagtcccgcc acgacctgtg
      901 gtccgacacg agcccgatgg tcgatgcggg aatcgacctc ctgatcgact cgatcgtcgg
      961 cgccgcaccg aatccatcgt tcgtcggcga cggccgcggc cgcctgacca acgaggtgct
     1021 ccccgccgcg ctcaccgaca gcgggcgccc gcggagcctc gcttcgtcgt tgtactactt
     1081 gttgagcaac gtcgaatccg ttcgtcagct cttctccgcc gactcgtggc gagcccttca
     1141 gcgaatcacc gaacggtacc ggtcactgct gaacgacggc aacgtgtcga ccacccagga
     1201 cgcactcgac acgttctcac cgttgctcga gctgtcggcg ttgatcgccg agtcgatggt
     1261 gcgagaccca ggatggtggt tcctcgacct cgggcgtcgc gtcgagggcg cccaactcgc
     1321 gaccggctcg atcggagcga cgttgcagcg cgacccgccg gaggagatcg cagcctcggt
     1381 gtggaacgcg ctcctgggca cctgggacgg ggtctttgcg tatcgacgtc gctaccgaag
     1441 cgacgtcgat ccgtcgctcc tcatcgacct gttgctgtgc gacacctcga acccgcattc
     1501 ggtatgtggc aatctcgccg ggttgacgcg ggctctcgat cacttgcact cgttgcgcgg
     1561 cgccggaccc aaccggtcgc cggcggcgca acagtgtgcg gcgctgctcg agtcgctcca
     1621 gggcgcgaat gtggaggcgc tcgcggcggt gggagcagac cgaacccgcc gctcgttgag
     1681 cgagttcacc accggcctca ccacggccat cgacgccgtg gccgaggaca tcgacctcac
     1741 gtacttcgtg caactcagct cgcggcatct tgaagggtcc cgcctaccca ccgaggctgc
     1801 cggcgtggcg tccgttggcc aggcaagcgc atgagatttc gggtgtcgca cacgacggca
     1861 taccgcagtc ggctcccggt cgtgatgggc tatcacctcg cctatctcac cccacgggac
     1921 tgtccgggac aacgagtgcg gtcccacgaa ctcctcgtca ctccgacgcc agcggaccgt
     1981 gcgggccatg tcgacgtgtt cggcaaccga ttcacgtact tctgcatcga agaacctcac
     2041 gtgttgttcg aggtcaccgc gacctcggag gtggaggtct ccggccggcg tcgcttcgcc
     2101 gaccctcagg cggattggca ttccggcgtg gaacgcgcca ccgaccctct cctcgccgac
     2161 ttcgtggcgt tcagcgactt cgtcacctcg tcgccgtcgg ttcgcgacta tgcagcgctg
     2221 tcgtttcctc cggggcgccc gttgctcgag gcgctcgagg agctcaatcg ccgcatcttc
     2281 gatgacttcg cctatgaccc cggctggacg acggtgtcga caccggtgga ggtggtgttc
     2341 gcaacacgtc gcggcgtgtg ccaggacttc gctcatctca tgttggcgtg cgtacggtcg
     2401 atgggtctgg ccggcaggta tgtctcgggc tacctcgaga atcgccctcc ggatggaacc
     2461 gacgcgctcg caggtgccga tgcgtcacac gcctgggttt cggtgctgtc gggtgacggg
     2521 atctggatcg acctcgaccc caccaacggc ctcgtcgaac ccgcccacca catcaccgtc
     2581 gcgtgggggc gcgagtacag cgacgtcgca cccttgcgcg gggtggtaac cgcaccgggc
     2641 gacccggcga cgctgtcggt tgccgtcgag gtggaacgca tcgattaccg aagcccgcgc
     2701 cgccatcacc gcaacccgcg gtagcgttct gaggttcgaa atgggggcac ttcgttgaat
     2761 cagaacgaca caccacgggt caccaaatcc agccgcgacc acggcgaact gcgacgacgt
     2821 ttcgaaagct ggctgcgcga acagctcggc cacgacgcca acccggtcat cacgtcgttc
     2881 gaagctccgg agtccaacgg catgtcgagc gagacgatcc tggtgagcgc cgtgtgggat
     2941 tcacccgatg ggcgaggcga ccacgatctg gtggtgcgca tcgccccacc gagcgactcg
     3001 atgccggtgt tttcccgcta cgacctgagc ctgcaatacg acgtcatggc gctcgtcggc
     3061 gaacgaaccg atgtgccgat tccccggctg ttgtggaacg agccgtcccc cgaaccgctc
     3121 ggagcgccgt ttttcgtgat ggggcgaagc atcggcgacg ttccccccga tctcatgcca
     3181 tacaccttcg gcagttgggt gtcggaggca accgacagcg accgcgagcg gatggagcgc
     3241 acgacggtcg acgtgttgac caaggtcgcc gccatcgcag acccacaggt ttcggtgccg
     3301 agcctcgccc cggaaccgga cgatgggcga agcgcattgc gttgccacgt cgatgccgct
     3361 cgcgcctggc tggactggtc ggtcggcgac gaggcctcgc cgctgttgga ccgctgtttc
     3421 gaatggctcg aatccaactg gccctccgat gagggcgaaa ccgtgctgag ctggggcgac
     3481 gcccggatcg gcaacatcat gtatcgcgat ttcgaaccgg tggcggtact cgactgggaa
     3541 atggcggcgt tggggccacg cgaactcgac cttgcctggc tgatcttcct ccaccgcttc
     3601 ttcgacgaca tcgccgttca gatggggttg caggcaatgc ccgacttcct gcgacgcgac
     3661 cgcatggaat cgctctatga agaactgagc ggacattgcc cgcaaaacat ggactggtat
     3721 ctcatgtaca gcgcggtgcg ccacggcatc gtgttcacac gaatctcacg acggatggcg
     3781 catttcggcg accaggaaat gcccgaggac cccgacgacg ccatccttca ccgcgcagcc
     3841 ctcgaagcca tggtcgaagg aacttactgg tcctgagcga cccgattccg ataccgcaag
     3901 gggcggcgcc tcactacgat gcgcaacgtc gcgaacagcg accgacacga tgcccgggtg
     3961 gcgaaatggc agacgcagag ggcttaaacc cctcagctcc gaaaggggcg tgcgggttcg
     4021 agtcccgccc cgggcacgat gtgttttcaa gggttttgaa aaccgacatg gatgccaccc
     4081 tcctggctag cggcgaccgc cgatctggtg tgtcggcccc gacggtcgaa cgccgagcgt
     4141 gtccttgtgg tgccgggcgc tacttggcga gcggggactc gacggctgag tcccacccgg
     4201 cagggcaccc catctcgcct gcgcgacgac gaggaatcgc ctcaagcgac tcgaggatca
     4261 ggctccgcag cttgggcgcc cacgacgatc cgggcgagac agtggtcagg atctgcgctg
     4321 tgaccagcgc ggctccatag atgcgttcgc tctggccgtc gggcagcgtc gaaagccctg
     4381 ggatgcttgc cagccctgcc gtgctggctg gtgtgaagga ccgattccag ggcctcgagt
     4441 ggtgggcact ggtgttgcga acgatggtga gctgctcaag ccatcggacg agtgggtggt
     4501 gcttcttggc cttctcctgc tggcgcttgc tgagctttga caggtcgatg cgaacgccga
     4561 tccgctcggc gacagcccac tggagacgag cagggagccc ctcgtagagt cgcgagctgt
     4621 cggcgaagtc gaggtgatcg gccagtaccc acagcgggta tcgctttccg tattc
//
LOCUS       DAOOMA010000145         4668 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_22136_length_4668_cov_4.608064, whole genome shotgun sequence.
ACCESSION   DAOOMA010000145 DAOOMA010000000
VERSION     DAOOMA010000145.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 4668)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4668)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4668
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22136_length_4668_cov_4.608064"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..632
                     /locus_tag="PLP95_05220"
     CDS             <1..632
                     /locus_tag="PLP95_05220"
                     /inference="COORDINATES: protein motif:HMM:NF012318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HPB45237.1"
                     /translation="RVGPSVVAVNTEVVQRRGPFTQTGSGAGTGVILTSDGQVLTNAH
                     VVSGASRVTVTVVVDGEEVEYPATVIAGDSSNDLALLQVDADTDLPAATIGESSTAAV
                     GDDVVAIGNALALEGGMSVTRGIVSGLDRAITAGDAYSSEQLTGLIQTDAAISSGNSG
                     GPLVNAAGQVIGINTAVASSSSGTQASNVGFVIPIDKAMSIVEQMRQQI"
     gene            complement(693..1628)
                     /locus_tag="PLP95_05225"
     CDS             complement(693..1628)
                     /locus_tag="PLP95_05225"
                     /inference="COORDINATES: protein motif:HMM:NF015431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HPB45238.1"
                     /translation="MKFHQLSTFVAVVDHGSVTAAADALSVSQPAVSQTIRSLERDLG
                     VAVFDRIGRNVRLNAAGEALLPGARQALRDLQIARDAVASVSALRSGRLDLVSLPTLG
                     VSPVAELIGEFHRRHPDISVRLVEPESAARIGSVVASGEAEIGFTELTSQGAVPFDPA
                     TGPDRLESVELDHQVYVVVYHRSVELDEVHAPDGAPVPLTELASLPLVTAIEGTSTRR
                     LIDEAFAYVGVAPRIAVETELREVIAAVVAAGGGYSILPRVVAEAIGSPDGDIVIAQV
                     APPIRRRVGVIFRPGAMSPAGRAFLEVVEESKRRG"
     gene            1699..2469
                     /locus_tag="PLP95_05230"
     CDS             1699..2469
                     /locus_tag="PLP95_05230"
                     /inference="COORDINATES: protein motif:HMM:NF014035.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HPB45239.1"
                     /translation="MSPHLTLTIILAGLAVGVLYGIFGVGSAFATPMLSIIGVPGLAA
                     VSGPLPAMLPGSATGAWSYVQAGNVDWRVAKRSIIGAVPASILGAIASQWVGGPALIA
                     MSGVVLFVVGVRVLRPTRAAAVLDTPSWADRHPVLLSAAAALVGFLSGLLANGGGFLL
                     VPMFLLMVGLDMNKATGTSLVVATLLTVPTLVTRAFIGDIDWLVAGAFAIGMIPGTRI
                     GALSAARFDVARLQRAFGVLLLVFAVWFLARELPHLIA"
     gene            complement(2483..3733)
                     /locus_tag="PLP95_05235"
     CDS             complement(2483..3733)
                     /locus_tag="PLP95_05235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB45240.1"
                     /translation="MDLRNLGRGELAVLIPEWLLGGQLIDRAGMPHAIGAFGREVMGA
                     VAIDEWMGASPIYSKRMQRFLRFEGDTVECIFKGMQLDIGAPPQFMDFRYQLNSEYSG
                     EFWLDHCGALVDVEPMGEEYVVTMCHDIEDPTFDATAIATNPRAQIRPIHRPPREPAD
                     RHPHCHWTVIIDDSHPEVPFPEQATQMMRRAIATIELDEPDRSEDGLTDYSGPLLADI
                     VWSEFGRTTLVRLAEEIAVEWHLLAISFLEAVRARAESEKSALRMYRNQGIGIAGVMA
                     QRLKAAFDLAPDLDGLEAVFQLHPMMHPLAYTAMATRRDGDSLVVSFDRRAAATADEA
                     WMSFWGDGHLDALQAVATEVNQRFVVALDEASGADSLVVRITEGDAEVAVHSSVELTR
                     FSTGAGFVFEERSTPITITPRSRS"
     gene            3836..4552
                     /locus_tag="PLP95_05240"
     CDS             3836..4552
                     /locus_tag="PLP95_05240"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HPB45241.1"
                     /translation="MTEYATPHPTAPSPTELPAAVLWDLDGTLVDTEPAWMRAEVLLA
                     NRHGVNWGPQDALQLIGTSLPNAGRIFQDLGVPGSVAEIVDELIGVVVETLGNHIEWQ
                     PGAQRILAELRDAGVRQALVTMSYRDMADLIIAGAPADTLTTSVTGDEVTNGKPHPEP
                     YLAAADLLGVDIAQCVAVEDSPTGIASALASGARVIGVQRQVPVAPEPGLSRLASFDA
                     LTVDTFRRVASGETIDELLA"
BASE COUNT          762 a         1629 c         1509 g          768 t
ORIGIN      
        1 atcgcgtcgg cccctcggtc gtggcggtga acaccgaggt ggtccagcgt cgaggcccct
       61 tcacccaaac gggatcgggc gccggcaccg gggtcatcct cacctccgac ggacaggtgc
      121 tcaccaacgc ccacgtcgtc agcggagcat cccgggtgac cgtcaccgtg gtcgtcgacg
      181 gcgaggaggt cgagtacccg gcgacggtga tcgctggcga ttcgagcaac gacctggcgc
      241 ttctccaggt cgacgccgac accgaccttc ccgcggcgac gatcggggaa tcctccaccg
      301 ccgccgtcgg cgacgatgtc gtggcgatcg gcaatgcgct cgcccttgag ggcgggatgt
      361 cggtgacccg cggcatcgtg tcgggcctcg atcgagcgat caccgccgga gacgcctact
      421 cgagcgagca gctcaccggc ctcatccaga ccgacgctgc gatcagttcg ggcaactcgg
      481 gtggcccgct ggtcaacgct gccggccagg tgatcggcat caacaccgcg gtggcgtcct
      541 cgtcgagtgg cacccaggcc tccaacgtgg gcttcgtcat cccgatcgat aaagccatga
      601 gcatcgtgga acagatgcgc cagcagatct agagacctgc ctgcgggtgc cgacccgcaa
      661 cacgttcgcc cgggctcctc caccgggtgc gttcagcccc gccgtttcga ctcctcgacc
      721 acctcgagga atgctcgacc ggcggggctc atcgcacccg gacgaaagat gactccgacc
      781 cgccggcgaa tcgggggcgc gacctgcgcg atcacgatgt ctccatcggg ggaaccgatc
      841 gcctcggcca cgacccgagg caggatcgaa taccccccgc cggcagcgac gaccgcggcg
      901 atcacctcac gcagttccgt ctccaccgca atgcgaggtg cgacccccac gtaggcgaag
      961 gcctcgtcga tgagccgacg ggtcgaggtc ccttcgatgg ccgtgacgag cgggagtgac
     1021 gccagttcgg tgagcgggac cggcgccccg tcgggggcat ggacctcgtc gagttcgacg
     1081 cttcggtgat acaccacgac atacacctga tggtcgagtt cgaccgattc gagccggtca
     1141 ggcccagtcg ccgggtcgaa gggcaccgca ccttgcgagg tcagctcggt gaacccgatc
     1201 tcggcctccc cgctggcgac caccgatccg atccgggccg ccgattccgg ctcgacgagc
     1261 cggacgctga tgtcggggtg acgccggtgg aactcgccga tcagctcggc caccggagac
     1321 accccgaggg tcggcagcga caccaggtcg agccgtccgc tgcgcagcgc cgacaccgac
     1381 gccaccgcat cgcgggcgat ctgcaaatcc cgaagcgcct ggcgcgcacc gggcaacagc
     1441 gcctcgcctg cggcgttgag ccggacgttg cgtccgatcc ggtcgaagac cgcgacgccg
     1501 agatcgcgtt cgaggctgcg aatcgtctgg ctcaccgcgg gctgcgacac gctcaacgcg
     1561 tccgccgccg cagtgacgga accgtgatcg accaccgcca cgaaggtcga cagttggtgg
     1621 aacttcataa gtgaagctta tcgcttcaat catttaccgc tcttggacat atgagtcgaa
     1681 atcggtgatc cttgaagcgt gtccccccac ctcaccctga cgatcatcct cgcgggcctc
     1741 gccgttggcg tgttgtacgg catcttcggc gtcggatccg cattcgccac gccgatgctg
     1801 tcgatcatcg gcgttcccgg cctcgccgcc gtgtcggggc cgctgcccgc catgctcccc
     1861 ggatcggcga ccggcgcgtg gtcctacgtt caggccggaa acgtcgactg gcgggtcgcc
     1921 aaacggtcga tcatcggcgc cgtccctgcg tcgattctcg gggcgatcgc ctcgcagtgg
     1981 gttgggggcc cagcgctcat cgccatgagc ggggtggtgt tgttcgtcgt cggagtccgg
     2041 gtgttgcgcc cgacccgtgc cgccgccgtc ctcgatacgc cgagttgggc cgaccggcac
     2101 ccggtgctgt tgtcggcggc ggcagcgctc gtcggctttc tgtccggcct tctggccaac
     2161 ggcggggggt tcctgctggt gccgatgttc ctcctgatgg tcggcctcga catgaacaaa
     2221 gcgaccggca ccagcctcgt cgtcgccacg ctgctgaccg tcccaaccct ggtgacgcgc
     2281 gcgttcatcg gcgacatcga ctggctggtc gccggagcgt tcgcgatcgg aatgatcccc
     2341 ggaacgcgca tcggtgcgct cagcgcggct cgattcgacg tcgcccgact ccagcgagcc
     2401 ttcggggtgc tgttgttggt cttcgccgtg tggtttctcg cccgcgaact cccgcacctg
     2461 atcgcctgag tagcgcgaac ctctaggacc tcgaccgtgg ggtgatcgtg atcggggtcg
     2521 agcgctcctc gaagacgaat cccgcgccgg tcgagaacct cgtcagctcg accgaactgt
     2581 gcaccgccac ctcggcgtct ccctcggtga tgcgcacgac gagcgagtcg gcacccgacg
     2641 cctcatcgag ggccaccacg aagcgctggt tcacctcggt ggcgacggcc tgcagcgcgt
     2701 caaggtggcc gtcgccccag aacgacatcc acgcctcgtc cgcggtcgca gcggcccggc
     2761 gatcgaagct caccaccaac gagtcgccgt cacgacgcgt cgccatcgcg gtgtaggcca
     2821 gcgggtgcat catcgggtgc agctggaaca ccgcttcgag gccatcgagg tcgggggcca
     2881 gatcgaaggc cgctttgagg cgctgggcca tcacccctgc gatgccgatg ccctgattcc
     2941 ggtacatccg cagcgccgac ttctccgact cggcccgtgc ccgtaccgct tccaggaacg
     3001 agatcgccag caaatgccac tcgacggcga tctcctcggc gaggcgaacc agcgtcgtgc
     3061 gaccgaactc gctccacacg atgtcggcca ataacggccc cgaatagtcg gtgagcccgt
     3121 cctcgctgcg atcgggctcg tcgagttcga tggtggcgat cgcccgccgc atcatctgag
     3181 tggcctgctc aggaaacggg acctcggggt gcgagtcgtc gatgatcacg gtccagtgac
     3241 agtgcgggtg gcgatccgcc ggttcgcgag gcgggcgatg aatcggacga atctgggcgc
     3301 gcggattggt ggcgatggcg gtggcatcga aggtcgggtc ctcgatgtcg tgacacatcg
     3361 tcacgacgta ctcctccccc atcggttcaa cgtcgacgag cgccccgcaa tggtccagcc
     3421 agaactctcc ggagtactcc gagttgagct ggtagcggaa gtccatgaac tgcggcggcg
     3481 ctccgatgtc gagctgcatc cctttgaaaa tgcactcgac ggtgtcgccc tcgaaacgca
     3541 ggaatcgctg catccgcttg gagtagatcg ggctggcacc catccactcg tcgatcgcca
     3601 cggcccccat gacctctcgg ccgaacgcac cgatggcatg gggcatgccg gcgcgatcga
     3661 tgagttgacc gccgagtagc cactcgggga tcaacaccgc gagctcgccc cggccgagat
     3721 tcctgagatc catgggcgaa gcgtagccca gccggacctt caggtttaca cctgttcata
     3781 ttttgtatcg cagcgtccgc tcacccaatc tcgaagcgtc gaccacactg gtgcggtgac
     3841 tgagtacgcg accccgcacc cgaccgcccc ctcgccgacc gaacttccgg ccgcggtgtt
     3901 gtgggatctc gacggcaccc tcgtcgacac cgaaccggca tggatgcgcg ccgaggtgtt
     3961 gctggcgaat cgccacgggg tgaactgggg gccacaggac gcgctgcagc tcatcggcac
     4021 ctcgctgccc aacgccggtc ggatctttca ggacctcggg gtgcccggct ccgtcgcaga
     4081 gatcgtcgac gagctgatcg gcgtggtggt cgagaccctc ggcaaccaca tcgagtggca
     4141 gcccggggcg cagcgcatcc tcgcggagct acgagatgcc ggagttcgcc aagcactggt
     4201 cacgatgagc tatcgggaca tggccgacct catcatcgcc ggcgctcccg ccgacacgtt
     4261 gaccaccagc gtcaccgggg acgaggtgac caacggaaag ccccaccccg agccctacct
     4321 tgccgccgcc gacctcctcg gagtcgacat cgcacagtgc gtcgcggtcg aggactcccc
     4381 caccgggatc gcgtcggcgc tcgcctccgg ggcgcgggtg atcggcgtgc agcgtcaggt
     4441 gccggtcgcg ccggaacccg ggctctcgcg cctcgcgagc ttcgacgccc tcaccgtcga
     4501 tacctttcgc cgcgttgcca gcggcgaaac gatcgacgaa ctgcttgcct aatctacgga
     4561 ttcttcggcc aggagccgac gtcgaaagag atcgagcacg agatcgagtg cgtcacgggt
     4621 cggttggccg ggcgcgtcga tgaggtgctc ggtcaacacc ccgtgagg
//
LOCUS       DAOOMA010000146         4665 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_22155_length_4665_cov_3.946855, whole genome shotgun sequence.
ACCESSION   DAOOMA010000146 DAOOMA010000000
VERSION     DAOOMA010000146.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 4665)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4665)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4665
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22155_length_4665_cov_3.946855"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(155..496)
                     /locus_tag="PLP95_05245"
     CDS             complement(155..496)
                     /locus_tag="PLP95_05245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB45242.1"
                     /translation="MNRRVKKLSSVAALVVLLAAGACSRPDEDPPVRITGDPTGANPG
                     FTTSTTSTTEPPVTTTTIFWAPNGPGGSMSSESTEPAESEADESAETTAGGAPSSSTT
                     SMVEASGNSDN"
     gene            complement(608..1117)
                     /locus_tag="PLP95_05250"
     CDS             complement(608..1117)
                     /locus_tag="PLP95_05250"
                     /inference="COORDINATES: protein motif:HMM:NF020297.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016840 - carbon-nitrogen lyase activity
                     [Evidence IEA];
                     GO_process: GO:0016051 - carbohydrate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formaldehyde-activating enzyme"
                     /protein_id="HPB45243.1"
                     /translation="MWNGMLVGSGSEGSGSSIALVDVALGDDKSTVAQAWLNGFASPG
                     GSGAPFLITVSPGVPVKPFALFVPKVRIGTRDHFEATHGPAHAGVALGILDAVRTGVV
                     PVDVAERAFLICSVWVDEGASTAHSDALVANNRSATLEALDNAGHEWPEAASFLEAPT
                     QVRNLYYQG"
     gene            complement(1174..2628)
                     /locus_tag="PLP95_05255"
     CDS             complement(1174..2628)
                     /locus_tag="PLP95_05255"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HPB45244.1"
                     /translation="MSSAPSSIRPAYQLVIGGEQCDAADGNTFTVENPATGEALATIA
                     QAGSADLDRALRIATEEFDRGTWRRTNATARGKVLLRASQLFRERAEEFAMAEVHGAG
                     HPIGNARWEAGAMADVFEFFAGAANKHYGQVIPVQDDGLDVALRDPIGVCGLIVPWNF
                     PMLITSWKVAPALACGNPVVIKPASLTPISALLMAEVLVEAGVPAGAISVIPGPGATV
                     GDALARDPRVAKLSFTGDSSTGAQIMRASSDNITRLSLELGGKSACIVFDDADLDTAI
                     ASTPLSVFDNTGQDCCARSRFLVQRSVYDRFVEGFVAGTEALVVGDPTDEATQLGPMI
                     SAGQRRTSLEYLEIAAAEGATRVCGGEATGPGHYLSPAVLTGVDNAMRVAQEEIFGPV
                     ASIIAFDDEDDAIRIANDSPYGLSGSLWTRDMGRAIRVTRAVRTGVMSVNSARSVRTE
                     APFGGYKRSGFGRELGMAAMEHYTETKNVFFSAE"
     gene            2719..3495
                     /locus_tag="PLP95_05260"
     CDS             2719..3495
                     /locus_tag="PLP95_05260"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020379021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HPB45245.1"
                     /translation="MGRLDDKVAVITGGGAGMGRIACERFASEGAKVVVADLDAALAA
                     EVVEAITASGGEAASAIVDVRDAQQVEAMIATAVERWGGLDILYNNAGVSPGDDDTPL
                     TTSEETWQFTFDVNVKGLFLCCKYGIDAMLASGGGSIINVASFVAHMGAATPQIAYTS
                     SKGAVLAMTREIAAIYARQGIRANALCPGPVLTPLLAKFLSDAEKRERRLVHIPMGRF
                     GEAEEMVNGALFLASDESSFMTGQSLLIDGGITSTYTTPL"
     gene            3513..>4665
                     /locus_tag="PLP95_05265"
     CDS             3513..>4665
                     /locus_tag="PLP95_05265"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamate-ammonia ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase family protein"
                     /protein_id="HPB45246.1"
                     /translation="MLTTERLRDLHDLGEIDTVLVVFPDMQGRFMGKRVVASFFLAEL
                     ERNGGAIEACDYLLAVDVDMNVLDGFRFANWETGYGDFVCRPDLSTLRLVPWLERTAL
                     VIGDLVDHHGSPIEVSPRALLRRQIDRANRLGYDVKAGTELEFFLFQESMEQAWKQDF
                     RDLTPSSPYIIDYHILNTSIDEGLIRKIRNDMMGAGIPVENSKGEAARGQHELNLRFD
                     DALVMADNHTVYKNGAKEIAQLAGRAITFMAKFRMDESGSSCHIHSSLWDRETDRSVM
                     ADESGAGLSDTFRWYLGGLLSTAADFAICWAPYINSYKRFQPGSWAPTAVGWGTDNRT
                     LGFRVVGHGQGMRVESRIPGADCNPYIALAATIAGGLHGVENRIEPPDAY"
BASE COUNT          789 a         1611 c         1500 g          765 t
ORIGIN      
        1 gaacgcccgc ggatgcacct cgaaggtctg atatggcggc ggttcgaccg cgccgagctg
       61 gggacgagcg atgagtgggt ccacgaaggt gaccgccttc aaccaggtat caacatctgg
      121 tgcgggcgac gcgagcgacg ctgtcgcagc gacgtcagtt gtcggagtta cccgacgcct
      181 ccaccatgga ggtcgtcgac gacgacgggg ccccgcccgc tgtcgtctcg gccgactcgt
      241 ccgcttccga ctcggccggc tcggtcgact ctgaactcat cgatcctccg ggcccattgg
      301 gggcccagaa aattgtggtc gtggtcacgg gaggttcggt ggtcgaggtg gtcgaggtgg
      361 tgaacccggg gttggctccg gtcgggtctc cggtgatccg caccggcgga tcctcgtcgg
      421 ggcgagagca ggctccggcg gcgagcagga ccacgagagc ggcgacgctg ctgagcttct
      481 tcacacgacg attcattcga ttctctccag agtgatccgg gttctcggga cgtcacactc
      541 tggcccacac cgcctgcggc gcgccagatg acccgaggtg gtccggctgg tcgttcagcg
      601 tagccgctca accctggtag tagaggtttc tcacctgggt gggcgcttcc agaaaactgg
      661 ccgcctcggg ccactcgtga ccggcgttgt cgagcgcctc caacgtggcc gagcggttgt
      721 ttgccaccag cgcatcgctg tgggcggtcg atgcgccttc gtccacccac accgaacaga
      781 tcaaaaaggc ccgctctgcc acatcgacgg gtaccacgcc ggtgcgaacg gcgtcgagaa
      841 ttcccagcgc aaccccggcg tgggccgggc catgggtcgc ctcgaaatga tcgcgggtcc
      901 cgatacggac cttcggcacg aacaaggcga acggcttgac cggaaccccg ggagaaacgg
      961 tgatcagaaa cggtgcaccc gaaccacccg gtgacgcgaa tccattgagc caggcctgag
     1021 cgaccgtgct cttgtcgtcg ccgagcgcca cgtcgacgag cgcgatcgac gatccgctcc
     1081 cctcgctgcc cgaacccacc aacatcccgt tccacatgga cggcgacgat atcggctttc
     1141 gggccctgcg cctactccac gcccactcgg ctcctactcg gcgctgaaga acacgttctt
     1201 ggtttcggtg tagtgctcca tcgccgccat tccgagctcg cgaccgaacc cggaacgctt
     1261 gtagccgcca aagggcgcct cggttcgaac gctccgcgcg ctgttcacgc tcatcacccc
     1321 ggtgcgcacc gcacgggtca cccgaatcgc acgccccata tcgcgggtcc acaacgaccc
     1381 cgacaggccg taggggctgt cgttggcgat gcggatggca tcgtcctcgt cgtcgaaggc
     1441 gatgatcgac gcgaccggcc cgaagatctc ctcctgggcg acgcgcatcg cgttgtcgac
     1501 cccggtgagc acggccggac tcaggtagtg acccggcccg gtcgcctcgc cgccgcacac
     1561 ccgcgtcgcg ccctccgccg cggcgatctc gagatattcc aggctggtcc gccgctgccc
     1621 ggcggagatc atcgggccga gctgggtggc ctcgtcggtc gggtcgccca ccacgagggc
     1681 ctcggtgccg gcgacgaacc cctcgacgaa tcggtcatag acgcttcgct gcaccaggaa
     1741 tcggctgcgg gcacagcagt cctggccggt gttgtcgaac accgacagcg gagtcgaggc
     1801 gatcgcggtg tcgaggtcgg cgtcgtcgaa cacgatgcac gccgatttgc caccgagttc
     1861 gagcgacagc cgggtgatgt tgtcgctcga cgcccgcatg atctgggcgc cggtcgaact
     1921 gtcgccggtg aacgagagtt tcgccacgcg gggatcgcgg gcaagggcgt cgcccaccgt
     1981 ggcgccggga cccggaatca ccgagatggc accggcggga accccggcct cgacgagcac
     2041 ctcggccatc aacagggcgc tgatcggggt gagcgacgcc ggtttgatca ccaccgggtt
     2101 gccgcacgcg agcgcggggg cgaccttcca gctcgtgatc aacatgggga agttccaggg
     2161 cacgatcagg ccgcacaccc cgatcgggtc gcgcagggcg acgtcgagtc cgtcgtcttg
     2221 caccgggatg acctgcccgt agtgcttgtt ggccgcaccg gcgaagaact cgaaaacatc
     2281 ggccatcgcg cccgcctccc agcgggcatt gccgatcggg tgcccggcac cgtgaacctc
     2341 ggccatcgca aactcctcgg cgcgttcgcg aaacagctgg ctggcccgca acagcacctt
     2401 gccccgtgcg gtcgcgttgg tccgccgcca cgtcccacgg tcgaactcct cggttgcgat
     2461 gcgcagcgcg cgatccagat cggccgagcc ggcctgcgcg atggtggcga gcgcctcgcc
     2521 cgtcgccgga ttctcgacgg tgaacgtgtt gccgtcggcg gcgtcacact gctcgccgcc
     2581 gatcacgagc tggtaggcgg gacgaatcga ggatggcgca gatgacatgg ccgaatcgta
     2641 tccccactcg gtattgtccc cgtgcggccg tcgcggcccg ccacccagga tcccccatcc
     2701 cactttcagg agcaaaacat gggtcgtctc gacgacaagg tcgctgtcat caccggtgga
     2761 ggcgccggca tgggccgcat cgcgtgcgag cgattcgcat cggagggcgc gaaggtcgtc
     2821 gtcgccgacc tcgacgccgc gctcgccgcc gaggtcgtcg aggccatcac cgcatcgggc
     2881 ggcgaggccg cgtcggccat tgtcgatgtc cgcgacgcac aacaggtcga ggccatgatc
     2941 gccaccgccg tcgagcgctg gggcggcctc gacatcttgt acaacaacgc cggggtgtcg
     3001 cccggcgacg acgacacccc gctcaccacc tccgaggaga cgtggcagtt caccttcgac
     3061 gtgaacgtca agggcctgtt cctgtgctgc aagtacggaa tcgacgccat gttggcctcc
     3121 ggcggcggct cgatcatcaa cgtcgccagc ttcgtcgccc acatgggcgc cgccaccccg
     3181 cagatcgcct acacctcctc aaagggagcg gtgttggcca tgacccgcga gatcgccgcg
     3241 atctatgccc gacagggcat tcgagccaac gcactctgcc ccggcccggt gctcaccccg
     3301 ctgctggcca agttcctctc ggatgcagaa aagcgcgagc gtcgcctcgt gcacattccg
     3361 atgggccgtt tcggcgaagc cgaggagatg gtcaacgggg cgctgttcct cgcgagcgac
     3421 gagagctcgt tcatgaccgg acagtcgctg ctgatcgacg gcggcatcac ctccacctac
     3481 accaccccgc tgtaagcgcc gagaggacct tcatgctcac caccgaacgc cttcgggacc
     3541 tccacgacct cggcgagatc gacacggtgc tcgtcgtgtt tcccgacatg cagggccgtt
     3601 tcatgggcaa gcgggtggtg gcctcgttct tcctggccga actcgaacgc aacggcggag
     3661 cgatcgaggc ctgcgactac ctgttggccg tcgacgtcga catgaacgtt ctcgacggtt
     3721 tccggttcgc caactgggag accggctacg gcgacttcgt gtgccggccc gacctgtcga
     3781 ccctgcggct cgtgccgtgg ctggaacgca cggcgctggt gatcggcgac ctcgtcgatc
     3841 accacggatc cccgatcgag gtgtcgccgc gggcgctgct tcgccgccag atcgatcggg
     3901 ccaaccgact cggctacgac gtcaaggcgg gcaccgaact cgagttcttc ttgttccaag
     3961 agtcgatgga acaggcgtgg aagcaggact ttcgcgacct caccccctcc agcccctaca
     4021 tcatcgacta tcacatcttg aacacctcga tcgacgaggg gctcatccgc aagatccgca
     4081 acgacatgat gggcgccggt atcccggtgg agaactccaa aggcgaagcc gcccgcggcc
     4141 aacacgagtt gaacctgcgt ttcgacgacg ccctcgtcat ggcagacaac cacacggtct
     4201 acaagaacgg cgccaaggag atcgctcaac tcgccgggcg ggccatcacg ttcatggcga
     4261 aattccgcat ggacgagtcg ggaagctcgt gccacatcca ctcgtcgttg tgggaccgcg
     4321 agaccgaccg ttcggtgatg gccgacgagt ccggcgccgg cctgagcgac acgttccgtt
     4381 ggtacctcgg cgggttgcta tcgaccgcgg ccgacttcgc catttgctgg gctccctaca
     4441 tcaactccta caagcggttt cagcccggat cgtgggcacc caccgcggtc ggctggggca
     4501 ccgacaatcg cacgctcggc ttccgggtgg tggggcatgg tcagggcatg cgggtcgaga
     4561 gccgcatccc gggtgccgac tgcaacccct acattgcgtt ggccgcgacg atcgccggtg
     4621 gactccacgg ggtggagaac cgcatcgagc cgcccgacgc gtatc
//
LOCUS       DAOOMA010000147         4655 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_22213_length_4655_cov_3.366304, whole genome shotgun sequence.
ACCESSION   DAOOMA010000147 DAOOMA010000000
VERSION     DAOOMA010000147.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 4655)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4655)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4655
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22213_length_4655_cov_3.366304"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..242)
                     /locus_tag="PLP95_05270"
     CDS             complement(<1..242)
                     /locus_tag="PLP95_05270"
                     /inference="COORDINATES: protein motif:HMM:NF013168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HisA/HisF-related TIM barrel protein"
                     /protein_id="HPB45247.1"
                     /translation="MKLYPAIDLRGGRCVRLYQGDYDRETHYGDDPVSQARQFADAGA
                     PWIHVVDLDAARSGDATNREVIAAICAAVEVPVQSGG"
     gene            complement(304..927)
                     /gene="hisH"
                     /locus_tag="PLP95_05275"
     CDS             complement(304..927)
                     /gene="hisH"
                     /locus_tag="PLP95_05275"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020376913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HPB45248.1"
                     /translation="MKLAVLDYGIGNLRSAQKAFEHVGAEARLTADPAEVEAADAVAL
                     PGVGAFGACVDALVGSGLDRVALGAIAAEMPFIGICVGMQLLYAGSEESPDHSGLGVF
                     DSTISLLPPTVKRPQMQWNRLTPTVADHPMFVGLEDPWVYFVHSYFATVDADTVATCE
                     YGATVGAAVARDNVWATQFHPEKSAATGLAILSNFLRWAEPRRSTIA"
     gene            complement(924..1559)
                     /gene="hisB"
                     /locus_tag="PLP95_05280"
     CDS             complement(924..1559)
                     /gene="hisB"
                     /locus_tag="PLP95_05280"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase HisB"
                     /protein_id="HPB45249.1"
                     /translation="MSTDPMSPDPRPARTAPRTVRRSRTTKETDITIELNLDGTGVVD
                     VSTGIPFFDHMLDQIGRHGGFDLTVRAEGDLHVDGHHTVEDTGILLGEVLREALGDKA
                     GVRRFASGLYPLDEALIGVALELSGRRYLVWNVTFGEILPLGNPPFNPEMTEHFFRSL
                     ADSSAVTLHVTSKGGVTAHHIIEATFKGVARALRDAVRVEGDQLPSTKGVL"
     gene            complement(1556..2638)
                     /gene="hisC"
                     /locus_tag="PLP95_05285"
     CDS             complement(1556..2638)
                     /gene="hisC"
                     /locus_tag="PLP95_05285"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020376916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HPB45250.1"
                     /translation="MSRPKVRDDVAMMEGYHSPQLDVSIRLNTNEAPQPPPPEFTRRL
                     AEAIGSIEWHRYPSRSASALRARIAEVEGVEADQVFAANGSNEVLQTVLLTYGGPGRT
                     ALTFEPTYALHSHLSRIAGTAVVEGERTDEFELDLDVVETLIARHRPAVTFLCSPNNP
                     TGRFESEATIRRVLELVEAVDGLLIVDEAYGQFAPWSAKELLSEDRNLVVTRTYSKTW
                     SMAAARLGYALAPSWLVAELDKVVLPYHLDASKQIAGLLALDYLDDMSARVAALVEER
                     GRVQSALADLDVDQWESGANFILFRPRHRSGAQVWQDLVDRSILVRNCASWPRLDDCL
                     RLTLGTAEENTAFLDALAEILETPTP"
     gene            complement(2635..3978)
                     /gene="hisD"
                     /locus_tag="PLP95_05290"
     CDS             complement(2635..3978)
                     /gene="hisD"
                     /locus_tag="PLP95_05290"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF013013.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HPB45251.1"
                     /translation="MLNRLDLRHVDRDVIAHLPRPEVAGDEPVAAVRSILAAVREQGD
                     EALLRFTREFDGIEMESLRVSAEQLTAAFERTPAEVREALAVAAGRIEAFHRTQVHGE
                     TTYGDGGYIRSFNVAVDRAGCYVPGGRAAYPSTVLMTAIPAKVAGVGSVVVCVPPDRT
                     TGAVTDVTLAACLVAGVDEVYAVGGAQAIAAMAYGTDTIAAVDVICGPGNKYVAIAKR
                     EVAGVTGIASAFAGPSEIVVVADSSAPAAFAAVDVVVQAEHGPDGLAWLVTWDEAVAD
                     AVSAEVERITEASPRRDEIVSTLATAGYAVLCRDAEQAMEVANAIAPEHLQIMTADPE
                     ALVGLVRNAGAIFCGPWSPASMGDYVAGPSHVLPTDGTARFSSALTVADFQKHHHVIT
                     FTAEQSAALAPVVDVLASAEGLDAHALSARMRLEAAAAVNPNATHPNAAQAGERP"
     gene            4047..4247
                     /locus_tag="PLP95_05295"
     CDS             4047..4247
                     /locus_tag="PLP95_05295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB45252.1"
                     /translation="MSSSADLASVASTLEQLMARVGGAVDEFTGTADEDVSIALMDVE
                     RSLRSANRRLDRLVKELRRRGL"
BASE COUNT          771 a         1570 c         1568 g          746 t
ORIGIN      
        1 cccccggact gcaccggaac ctccaccgcc gcgcagatgg cggcgatgac ttcgcggttg
       61 gtggcgtctc ccgagcgggc agcgtcgagg tcgaccacgt ggatccacgg ggcgccggca
      121 tccgcgaact ggcgagcctg actcaccggg tcgtcgccgt agtgggtttc tcggtcgtag
      181 tcgccctggt acagccgcac acagcggccg ccgcgcaggt cgatggcggg atacaacttc
      241 atggcctgtg cctcatcagg gtcggggatg gcgtcacgtc gctgcggatc acgtcgctgc
      301 ggatcaggcg atcgttgatc ggcggggctc ggcccagcgc agaaagttcg acaagatcgc
      361 caaaccggtg gcggcggatt tctcggggtg gaactgggtc gcccagacat tgtctcgggc
      421 aaccgcggca ccgacggtcg ctccgtactc acatgtggcc acagtatcgg catcgacggt
      481 ggcgaaatag ctgtgcacga aatacaccca cgggtcctcg aggccgacaa acatcggatg
      541 gtcggccacg gtcggcgtca aacggttcca ctgcatctgt ggacgcttca cggtgggcgg
      601 caacagcgag atcgtcgagt cgaacacgcc caggcccgaa tggtcgggcg actcctcgct
      661 gcccgcatac agcagctgca tgcccacgca gatgccgatg aagggcatct ctgcggcgat
      721 ggcgccgagc gccacccggt cgagccccga ccccacgagc gcgtcgacac acgcgccgaa
      781 ggcacccacc ccgggcaggg ccaccgcatc ggccgcctcc acctctgccg gatcggcggt
      841 gagtctggcc tcggcgccga cgtgttcgaa cgccttctgg gccgaacgca ggttgccgat
      901 gccgtagtcg aggaccgcca gcttcacagc acgcccttgg tggagggcaa ctggtcgcct
      961 tccactcgca ccgcgtcgcg cagggcacgc gccaccccct tgaaggtggc ctcgatgatg
     1021 tgatgcgccg tgaccccacc cttgctggtg acgtgcaacg tgacggccga ggagtcggcc
     1081 aacgatcgga agaagtgctc ggtcatctcc gggttgaacg gcgggttgcc gagcggcagg
     1141 atctcgccga aggtcacatt ccagacgaga tagcgacggc ccgacagctc gagggccacc
     1201 ccgatgagcg cctcgtcgag cggatagagc cccgacgcga accgtcgcac cccggccttg
     1261 tcgccgagag cctcgcgcag cacctcaccg aggagaatgc cggtgtcctc gacggtgtga
     1321 tggccatcga cgtgcagatc gccctcggcg cgcacggtga ggtcgaaccc accgtggcgg
     1381 ccgatctggt cgagcatgtg gtcgaagaac gggatgccgg tggacacgtc gacgaccccg
     1441 gtgccgtcga ggttcaactc gatcgtgatg tcggtttcct tggtggttcg gctgcggcgg
     1501 accgtgcgtg gggccgtgcg cgccggcctg ggatcggggc tcatgggatc ggtgctcatg
     1561 gggtcggggt ctccagaatc tcggcgaggg cgtcgaggaa cgccgtgttc tcctcggcgg
     1621 ttcccagggt caggcgcagg cagtcatcga gccgcggcca cgacgcacag ttgcgaacaa
     1681 ggatcgaacg gtcgaccaag tcctgccata cctgcgcgcc cgaacggtgc cgtggccgaa
     1741 acaggatgaa gttcgcaccc gattcccact gatcgacgtc gaggtccgcg agcgccgact
     1801 gcacccgacc gcgttcttcc acgagggcgg cgacacgggc cgacatgtcg tcgaggtagt
     1861 cgagggccaa cagtccggcg atctgcttcg aggcgtcgag gtgatacggc aacacgacct
     1921 tgtcgagttc ggccacgagc caagacggcg ccagcgcgta gccgagccgg gcagccgcca
     1981 tcgaccaggt cttggagtag gtgcgggtga cgacgaggtt gcggtcctcg ctcagcaatt
     2041 ccttcgccga ccacggcgcg aactgcccgt aggcctcgtc gacgatgagg agcccgtcga
     2101 ccgcctccac cagttcgagc acccgtcgaa tcgtcgcctc cgattcgaac cgcccggtcg
     2161 gattgttcgg cgagcacaga aacgtcaccg ctggacggtg gcgggcgatg agcgtttcga
     2221 ccacatcgag gtcgagttcg aactcgtcgg tgcgctcccc ttcgaccacc gccgtgccgg
     2281 caatccgact gaggtggctg tgtaacgcat aggtcggctc gaaggtgagc gcggttcgcc
     2341 ccggaccgcc gtaggtcaac agcacggtct gcagcacctc attggagccg ttggcggcga
     2401 agacctgatc ggcctcgacg ccctcgacct cggcgatgcg agcacgcagt gccgaagcgc
     2461 tgcggctcgg gtagcggtgc cactcgatcg acccgatggc ctcggccagg cgccgggtga
     2521 actccggggg cggcggttgc ggggcctcgt tggtgttgag ccggatgctc acgtcgagtt
     2581 ggggcgagtg gtacccctcc atcatcgcga cgtcgtcgcg taccttcggc cggctcatgg
     2641 ccgctccccc gcttgcgccg cgttcgggtg cgtcgcgttc gggttcaccg cggcggccgc
     2701 ttccaggcgc atccgagcgc tcagcgcgtg ggcgtcgagc ccctccgcgg aggcgagcac
     2761 gtcgacgacc ggagccaacg ccgccgattg ctcggcggtg aaggtgatga cgtggtggtg
     2821 cttttggaag tcggccacgg tgagcgccga gctgaaccgg gcggttccgt cggtgggcag
     2881 cacatggctc ggcccggcga cgtagtcacc catcgacgcg ggcgaccagg gaccacagaa
     2941 aatggccccg gcgttgcgca ccaggcccac gagcgcctcg ggatcggcgg tcatgatctg
     3001 caggtgttcc ggtgcgatgg cattggcgac ctccatcgcc tgttcggcgt ctcggcacag
     3061 caccgcgtag ccggccgtgg cgagggttga cacgatctcg tcgcgccgcg gcgacgcctc
     3121 ggtgatgcgt tccacctcgg cgctgacggc atcggccacc gcctcgtccc aggtcaccag
     3181 ccaggcgagc ccgtcggggc cgtgttctgc ctggacgacg acgtcgaccg cggcgaaggc
     3241 cgccggagcg gacgagtcgg cgacgacgac gatttcggag ggaccggcga aggccgatgc
     3301 gattccggtc acgccggcaa cctcgcgctt ggcgatggcg acgtacttgt ttcccggccc
     3361 gcagatcacg tcgaccgccg cgatcgtgtc ggttccgtag gccatcgcgg cgatcgcctg
     3421 tgccccgccg accgcataga cctcgtcgac cccggccacg agacacgccg cgagggtcac
     3481 gtcggtgacc gcaccagtgg tgcgatcggg tggaacgcac acgacgaccg aacccactcc
     3541 cgcgaccttg gccggaatcg ccgtcatcag caccgtggag gggtaggccg cccggcctcc
     3601 ggggacatag cagccggccc ggtcgaccgc gacgttgaac gaccggatgt atccgccgtc
     3661 gccataggtc gtctcgccgt ggacctgggt gcggtggaag gcctcgatgc gcccggccgc
     3721 cacggcgagc gcctcgcgta cctccgccgg agtgcgctca aatgcggcgg tcagctgttc
     3781 ggcgctcacc cgcagcgact ccatctcgat gccgtcgaac tccctggtga accgcagcag
     3841 cgcctcatcg ccctgctccc gtacggcggc gaggatcgag cgaacggcgg ctaccggttc
     3901 gtcaccggcc acctctgggc ggggaaggtg ggcgatcacg tctcggtcga cgtgacgcag
     3961 gtcgaggcga ttcaacacgg ctccaacgct accggttgag cgcgaccccc ctcgaatggg
     4021 tttgcctcgc tgctagacac gtcgccatga gttcctcggc cgacctggca tctgtcgctt
     4081 ccaccctcga acaattgatg gcccgcgtcg gcggggcggt cgacgagttc accggtactg
     4141 cggatgagga cgtgagcatc gcactcatgg acgtcgagcg gtcgttgcgc agcgcgaacc
     4201 gacgcctcga ccggctggtg aaggaactgc gtcgccgcgg cctctagccc accggccgtc
     4261 acatctggtc gattgcggca ggcaaaatca ccttcgagca aaaatgcgac cggcgcctcc
     4321 tgatggggcg ccggtcgggc gaaacgggtg gcgggatccc attatcgcct tctccggatg
     4381 gcggacatcc ggagcggtta aaaccgtact gccaaccccg aaacttgtcg agtcaagcgg
     4441 caattattcc tatgtgacgt ttgtcacata ggaacgaaac ctaagaaaaa cgccttacgc
     4501 cagacccccg gaattctggg gttttgtcac tgcacactgc cgacacattt tctccgcgta
     4561 cgctgacacc atgaccaaat ctcccccatt tgaccgcccc ccgcaggagc cggatcccag
     4621 cgcgattcga gccctcgccg agcgcttcgg cgggg
//
LOCUS       DAOOMA010000148         4648 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_22265_length_4648_cov_4.005443, whole genome shotgun sequence.
ACCESSION   DAOOMA010000148 DAOOMA010000000
VERSION     DAOOMA010000148.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 4648)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4648)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4648
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22265_length_4648_cov_4.005443"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..254
                     /locus_tag="PLP95_05300"
     CDS             <1..254
                     /locus_tag="PLP95_05300"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020379584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HPB45253.1"
                     /translation="SRSREYEADASAARLLGTGEPLACALEKLEIGAQSIPAEVNEAQ
                     AQAYIVNPLRGGGQSLARMFSTHPSTADRISRLRDQSWR"
     gene            complement(293..781)
                     /locus_tag="PLP95_05305"
     CDS             complement(293..781)
                     /locus_tag="PLP95_05305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012225403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HPB45254.1"
                     /translation="MPSFDISSEIDEQEVRNAVDQAQREVSNRFDFKGTDSSIELSPT
                     EITLRTVSADRLTALRQVLEEKFAKRKLSMKSLDYGKVEDAAGATVRQVATLVAGINS
                     ERAKDINKFIKTLGIKGLQSQTQGEQIRVIAKKRDDLQAVIAACKEHDFGIPLQFGNF
                     RD"
     gene            932..1017
                     /locus_tag="PLP95_05310"
     tRNA            932..1017
                     /locus_tag="PLP95_05310"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:966..968,aa:Tyr,seq:gta)
     gene            1103..1606
                     /locus_tag="PLP95_05315"
     CDS             1103..1606
                     /locus_tag="PLP95_05315"
                     /inference="COORDINATES: protein motif:HMM:NF015999.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HPB45255.1"
                     /translation="MDTVDWPDSVQRVRRRLAHLGYEGEVTMLAESARSAQQAADAVG
                     AEVGAIAKAIIFRRSGDDVPVLVITSGANRVDIAKVEELVGEVGKADAAYVRERTGFA
                     IGGVSPIGMDPDAVVLFDEDLTRFDTVWAAAGHPNSVFEATPAQLAAYAGARVEDIRV
                     DDGESDD"
     gene            1599..2747
                     /locus_tag="PLP95_05320"
     CDS             1599..2747
                     /locus_tag="PLP95_05320"
                     /inference="COORDINATES: protein motif:HMM:NF013232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB45256.1"
                     /translation="MTDMAGLGEELCDELEAIYERGHYPALTVQRPWSQHNPTLVGEL
                     ARPAALRWYLIREIERLAEAGATLSATASRARIALDDPRMFRQGDESNWDLKRKKLFL
                     FPPERMELSLNRLSHYTGTEAETFQRYVLFTNYHMHVEAFLEIFPDCVKPSRSNVQMP
                     AYHHVMDDHSGITLVNIGVGPSNAKTISDHVAVLRPDLMLMIGHCGGLRNRQDIGDFV
                     LASTYVRADQVLDDVLAGYVPIVSNFQLNQILHDELSRRRLPFRMGTVFTTSNRNWEF
                     NQAQPLEQISASRAVATDMESATIAANGFRYRVPSATLLVVSDKPLHGQPKLEGPAKE
                     FYEASKRQHVEVAISAVNEVNQRWPGGLPSADLRSTDEPLIGGPAEVD"
     gene            complement(2838..4118)
                     /locus_tag="PLP95_05325"
     CDS             complement(2838..4118)
                     /locus_tag="PLP95_05325"
                     /inference="COORDINATES: protein motif:HMM:NF013086.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HPB45257.1"
                     /translation="MNEGLDVDVVVVGAGLAGLSASIAAARTGASVVLVDARDEIGGR
                     ARSDRAGDAIVNHGPHALYSAGEATRLLREYGVSVAGSMPLQRGFGWWRHGERRRALD
                     RQATGGLLGVTTLLHSMRRRCVDEAQGRSLRQWLDAEVPADARPLVEMMLRTTTYAAD
                     PDHTDAAAALAQLRRGARGVRYLDGGWQRIVDGLVAVALELGVRIEHDRVVEVTAGDD
                     AVRVTASHNSIRAGAVVVATGGPGHAHRLTGQRSEQLGMWAKRARPVQAYCLEVVLAS
                     RTGRGTALYGTDVPLYLVDHATDARLAPDSQAVLHGLIYEPDLCPDLDPRAELETALD
                     AVEPGWRDRTIKIIERRRMVVAHDRPQPHASGADTPQIATADLPGVWFAGDWITAKGW
                     LADAAMSSGDDAGRAAATRATATTAAPSPLPFCR"
     gene            4201..>4648
                     /locus_tag="PLP95_05330"
     CDS             4201..>4648
                     /locus_tag="PLP95_05330"
                     /EC_number="1.13.12.-"
                     /inference="COORDINATES: protein motif:HMM:NF015047.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016703 - oxidoreductase activity, acting
                     on single donors with incorporation of molecular oxygen,
                     incorporation of one atom of oxygen (internal
                     monooxygenases or internal mixed function oxidases)
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HPB45258.1"
                     /translation="MNDVEEAPPRLRTALTDRLGIDHPILLAAMGDSAGGRLAAAVSN
                     AGALGMVGGGYADPVWLERELQLVGDARVGIGFVTFALDERPDTLRQALEAAPVAVQL
                     SFGDPRPYADAIKATGAALICQVQRDDEVDQAIEAGADVLIAQGQDS"
BASE COUNT          790 a         1574 c         1541 g          743 t
ORIGIN      
        1 tgagccgaag ccgagagtac gaagccgacg cctcggccgc ccgactgctc ggcaccggcg
       61 aaccgctcgc ctgtgcgctc gagaaactcg agatcggggc acaaagcatt ccggccgagg
      121 tcaacgaagc gcaggcacag gcctacatcg tcaatccgtt gcgcggaggc gggcagagtc
      181 tcgcccgcat gttctcgacc cacccgtcga ccgcggaccg catctcccgc ctgcgcgacc
      241 aatcctggcg ctgaactgcc ggccgcagcg ggctagtcgc ggtaagcgac ggctagtcgc
      301 ggaagttgcc gaactgcagc ggaatgccga aatcgtgctc cttgcacgcg gcgatcacgg
      361 cctgcagatc gtcgcgcttc ttggcgatca cgcgaatctg ttcgccctga gtctggctct
      421 gcaaaccctt gatcccgagg gtcttgatga acttgttgat gtccttggcc cgctcggagt
      481 tgatgccggc gaccagcgtc gccacctgtc gaaccgtcgc cccggcagcg tcttcgacct
      541 tgccgtaatc cagcgacttc atcgacagct tgcgcttcgc gaacttctcc tcgagcacct
      601 ggcgaagcgc ggtcagacgg tcggcgctga ccgtgcgcaa cgtgatttcg gtgggggaga
      661 gctcgatgct cgagtcggtg cccttgaagt cgaaacgatt cgagacctca cgctgggcct
      721 gatcgacggc attgcgcact tcctgttcgt cgatctcgga actgatgtcg aaactgggca
      781 cggagggctc cttcaaaaga cgctggccac gggcgggctg cggcgacgaa cgaggcgaga
      841 ttcccgcaac gcaggcggtt tcgccggata cggccaacca cgctatccaa tttcgcaacc
      901 gcgatcggtg gcgctaccat gtggcgtcct tgggtcagtg cccgagtggc caaagggagc
      961 aggctgtaaa cctgccggcg taagcctacg gaggttcaaa tccttcctgg cccaccaagg
     1021 atgcccacca aaccccgcct tcgggcgggg tttcgtgctg ttcggacgcg tgtcggcgac
     1081 cgccgcgacc aaactggaac ccatggacac tgtcgactgg cccgactcgg tgcagcgcgt
     1141 tcgccggcgg ctcgcccatc ttggttatga gggcgaggtg acgatgctcg ccgaatcggc
     1201 caggagcgcg cagcaagccg cggacgcggt gggagccgag gtgggggcga ttgcgaaggc
     1261 gatcatcttt cggcgcagtg gggatgacgt gcccgtgctg gtcatcacca gcggagcgaa
     1321 ccgggtcgac atcgccaagg tcgaagagct cgtcggcgaa gtcggcaaag cagatgccgc
     1381 ctatgtgcgg gaacggaccg gctttgcgat cggcggggtg agccccatcg ggatggaccc
     1441 cgacgccgta gtgctgttcg acgaggacct gactcgattc gacacggtgt gggcggccgc
     1501 cggtcacccc aactcggtgt tcgaggcgac gcccgctcag ctcgccgcat atgccggggc
     1561 gcgcgtcgaa gacatccggg tcgacgacgg cgagtcggat gactgacatg gccggcctcg
     1621 gcgaggaact gtgcgacgag ctcgaggcca tctacgaacg cggccactac ccggcgctga
     1681 cggtgcaacg accgtggtcg cagcacaacc ccacactcgt cggcgaactc gctcgacccg
     1741 ccgcgctgcg gtggtatctc atccgtgaaa tcgagcgact cgccgaggcc ggggcgaccc
     1801 tcagcgcgac cgcgtcacgg gcacgcatcg cgctcgacga tccccgcatg tttcgccagg
     1861 gcgacgagtc gaactgggac ctgaagcgca agaagctttt cctgttcccg cccgaacgca
     1921 tggaactctc gctcaaccgg ctgtcgcact acaccggcac cgaagccgag acgtttcagc
     1981 gctacgtgtt gttcaccaac taccacatgc acgtggaggc gttcctcgag atcttccccg
     2041 actgtgtgaa gccgagccgg tcgaacgtgc aaatgcccgc gtaccaccac gtgatggatg
     2101 atcactcggg catcacgctg gtcaacatcg gtgtcggtcc gtcgaatgcc aagacgattt
     2161 cggatcacgt ggccgtcctg cgacccgacc tcatgttgat gatcggccac tgcggcggac
     2221 tgcggaaccg ccaagacatc ggtgacttcg tgctggcgag cacctatgtg cgcgccgatc
     2281 aggtcctcga cgacgtcttg gctggctacg tgccgatcgt gtcgaacttc cagctgaacc
     2341 agatcctcca cgacgagttg agccgacgac gcctgccgtt tcggatgggc accgtgttca
     2401 cgacctcgaa ccggaactgg gaattcaacc aggcccaacc gttggaacag atctccgcat
     2461 cgcgggcggt ggccaccgac atggaatcgg ccaccatcgc cgccaacgga ttccgctacc
     2521 gggtgccctc ggcgacgctg ctcgtcgtga gcgacaagcc actgcacggt caaccgaaac
     2581 tcgagggacc ggccaaggag ttctacgagg cctccaagcg ccaacacgtc gaggtggcga
     2641 tctccgcggt gaacgaggtg aaccagcgtt ggccgggcgg attgccgtcg gcagacctgc
     2701 gatcgaccga cgagccgctc atcggcggtc cggcggaagt ggactgagcg cgcctcggtt
     2761 cgcgcccgca ccccgcgccc agcgccgcgc cccagtccgc gttctgtagg taatcgcggt
     2821 ggctatgacc gccgcgatta cctacagaac ggcagtggtg agggggcagc agtggtggcc
     2881 gtcgcgcggg tggcggcggc ccggccggcg tcgtcgccac tcgacatcgc cgcatctgca
     2941 agccaaccct tggccgtgat ccaatcgccg gcgaaccaca cgcccggcag gtccgcagtg
     3001 gcgatctgcg gggtgtccgc ccccgaggcg tgtggttgcg ggcgatcgtg ggcgacgacc
     3061 atgcggcggc gttcgatgat cttgatcgtg cgatcacgcc agcccggctc caccgcgtcg
     3121 agcgcggtct cgagttcggc gcgagggtcg aggtccgggc acaggtctgg ttcgtagatc
     3181 aacccgtgca gcaccgcctg cgaatccggc gcgaggcggg cgtcggtcgc gtggtcgacg
     3241 aggtagagcg gaacgtcggt tccgtacagc gcggtgccgc ggccggttcg tgaagccagg
     3301 accacctcca ggcagtacgc ctgaaccggc cgcgctcgct tcgcccacat gcccagttgc
     3361 tcgctgcgct gcccggtgag gcgatgggca tgtcctggtc cgccggttgc gacgacgacc
     3421 gcaccggcgc gtatcgagtt gtgtgatgcc gtgacgcgca ccgcgtcgtc gccggccgtc
     3481 acctcgacga ctcggtcgtg ttcgatccgc accccgagct caagcgcgac ggcgaccaga
     3541 ccgtccacga tgcgctgcca tcctccgtcg aggtacctca cgccgcgggc tccgcgccgc
     3601 aactgggcga gggccgccgc agcgtcggtg tggtcgggat cggcggcata agtggtggtc
     3661 cgcaacatca tctcgaccag tggccgggcg tcggcgggaa cctcggcatc gagccactgt
     3721 cgcagcgagc ggccctgcgc ctcgtcgacg catcgccgtc gcatcgaatg aagcagcgtc
     3781 gtgaccccca acaacccgcc ggtcgcttgg cgatcgagcg cacggcgccg ctcaccgtgg
     3841 cgccaccagc cgaacccgcg ctggagcggc atcgagccgg ccacgctcac cccgtactcg
     3901 cgtagcagtc gtgtcgcctc gccggcgctg tagagggcgt ggggtccgtg gttgacgatc
     3961 gcgtcgccag cgcggtcact gcgcgcccga ccgccgatct cgtcgcgcgc atcgaccaac
     4021 accaccgacg ccccggtgcg ggcagcggcg atggacgcgc tcaacccggc gaggcctgcg
     4081 cccaccacga cgacgtcgac gtcgagtccc tcgttcatcg cgacctcctg gcgttctgta
     4141 ggtactctca ttggctggcg caacagaact tacctacaga accaaagagg gaagggtccg
     4201 atgaacgacg tcgaggaagc accgccgcga ttgcggaccg ccctcaccga tcggctcggc
     4261 atcgatcatc cgatcctgtt ggcggccatg ggcgacagcg cgggtgggcg gctcgcggcg
     4321 gcggtgtcga atgccggtgc gctcggcatg gtgggcgggg gatacgccga ccccgtgtgg
     4381 ctggagcgcg aactccaact cgtgggcgac gcccgggtcg gcatcgggtt cgtgaccttc
     4441 gcgctcgacg aacgccccga cacgttgcgc caagcgctcg aggccgcacc cgtcgcggtg
     4501 caactgtcct tcggcgaccc gcgcccgtac gcggacgcca tcaaggccac cggtgccgcg
     4561 ctgatctgcc aggtgcaacg cgacgacgag gtcgaccagg cgatcgaagc cggcgccgac
     4621 gtgttgatcg cgcagggaca agactccg
//
LOCUS       DAOOMA010000149         4637 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_22334_length_4637_cov_3.860323, whole genome shotgun sequence.
ACCESSION   DAOOMA010000149 DAOOMA010000000
VERSION     DAOOMA010000149.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 4637)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4637)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4637
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22334_length_4637_cov_3.860323"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..902)
                     /locus_tag="PLP95_05335"
     CDS             complement(<1..902)
                     /locus_tag="PLP95_05335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012222739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ThiF family adenylyltransferase"
                     /protein_id="HPB45259.1"
                     /translation="MPSFRDLLNSTKGEIVEVSTSEAEELRDAGAIVLDVREADEFEQ
                     GSIPGAIFIPRGNLESNIENRISDKDAKIVVHCAGGVRSAFAAKTLAELGYRDVVSMA
                     GGFNKWKDEGREWSAPKVLTPEQRNRYQRHLLVPEVGEEGQQKLLGAKVFLLGAGGLG
                     SPAALYLAAAGVGTIGIVDMDVVDASNLQRQIVHNTDRIGMRKVDSARETIEKLNPDV
                     KVITYDQRLDASNIEEIISGYDIVVDGADNFPVRYMLNDASVKLGIPVAHGSIFRFEG
                     QVTVFHPLEGPTYRDMLPSPPPPE"
     gene            1103..1654
                     /locus_tag="PLP95_05340"
     CDS             1103..1654
                     /locus_tag="PLP95_05340"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR00636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HPB45260.1"
                     /translation="MQIYTRKGDDGTTGLYFGGRVPKDSPRPQAYGDVDETQSAIGLA
                     RAHAVQLGVHDVAELDRILIGVQRDLWVVMADLATGSDNRGKLTPGRTSVTAEMVTAL
                     EQLIDSTATRFEPPTEFVVPGGDVVSAALDVARTVCRRAERSAVAIDDPGSHATGYLN
                     RLSDLLWMLARWQEGSALTVRSI"
     gene            1728..3167
                     /locus_tag="PLP95_05345"
     CDS             1728..3167
                     /locus_tag="PLP95_05345"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF002073.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HPB45261.1"
                     /translation="MTTLKHARTLSTRTAVVARLVPSDALDAAGVDAALLKRLSFKGE
                     AGQSATVTSEGTVIRFVGLGASGEVNPTAIRKATANAVRELSGHASVAVDLGVLATSA
                     SLDLEAVVQAAGEGAALGAYRFTEYKAASDTPYVATVTLVAPGGKAGLTRAIAISDSV
                     NWARDLVNEPGGTLTPTVFAQRVKERASAAGLKVEVLTAKQIAAKGLGGLLAVNRGSV
                     EEPRFVKLTYNPATAAARKRPSVGLVGKGITFDSGGLSIKPVAGMMTMKCDMAGAAAV
                     VATMVALPALGVGVPVTSYTPLTDNMPGPDATRPGDIYRAHNGTTVEVLNTDAEGRLV
                     LGDALSIASDDGHRTIIDLATLTGACMVALGDQIAGLMGTDDELIEALKTAAERAGEK
                     VWHLPLPAEYRKLIDSPIADIKNIGGQYGGTLTAGLFLKEFVGDDIAWAHLDIAGPAF
                     TEAANDEISRGGTGFGVRTLIELLEARAR"
     gene            complement(3248..>4637)
                     /locus_tag="PLP95_05350"
     CDS             complement(3248..>4637)
                     /locus_tag="PLP95_05350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB45262.1"
                     /translation="PVGSPLLHVVDLLLPGPLMPVSRFVTMAELFAAVAGSVMLATGR
                     GLKTLRPIDVAVIPAGAVISAHTFPLMAWHTVDGLLVGMASLLMLRSGVRRSSVSAVA
                     IGGALAGFAPLVKQSYAAVPIIALAWFIVQVRRRDPLPRWRPLAVAAATGLWVLRVGP
                     ARLPVTVHLSGAHRAAAGLAALALVAMAWSVWDEALSLGGSWGAAVWWAAFATMVAAS
                     ASRGRFDDAGAALLALGWCGSLSWGYANVNLFAGALAAYVAVRGVDLVVRVVPKPSLA
                     VVAPSLAVVVIALSVACVAWASGVRGENTYRDLPESELHSHLSDAATAFGYIRTNVGT
                     ATYVKAAAECRRRHPSGGFAILPDNAVLPTIFGARNPLPGDWWYILELPSHRERIMAQ
                     VGEAGRRGDYLVLFQTFSLDALAHQSAMPTATADSTPFDYEGGMVRDIYEALPGDRVE
                     CGPFIGIYKPAG"
BASE COUNT          826 a         1623 c         1524 g          664 t
ORIGIN      
        1 atctccggcg ggggcggtga gggcaacatg tcgcggtagg tcgggccctc gagtgggtga
       61 aacaccgtga cctgcccctc gaagcggaag atcgacccgt gcgccaccgg gatgccgagt
      121 ttcaccgacg cgtcgttgag catgtagcga accgggaagt tgtcggcacc gtcgacgacg
      181 atgtcgtagc ccgagatgat ctcttcgatg ttggaggcat cgaggcgttg gtcataggtg
      241 atgaccttca cgtccgggtt cagtttctcg atggtttcgc gggctgaatc gaccttgcgc
      301 atgccgatgc gatcggtgtt gtgaacgatc tggcgttgca ggttggaggc gtcgacgacg
      361 tccatgtcga cgatgccgat cgtcccgaca ccagcggccg ccagatacag cgccgccggc
      421 gaaccgaggc caccggcgcc gagcagaaac accttggcac cgagcagctt ctgttgcccc
      481 tcttcgccca cctcggggac gagcagatgg cgttggtacc ggttgcgttg ctcgggggtc
      541 agcaccttgg gcgcactcca ctcgcggccc tcgtccttcc acttgttgaa cccacccgcc
      601 atcgacacga cgtcgcggta tccgagttcg gccagcgtct tggcggcgaa cgccgagcgg
      661 acaccaccgg cgcagtgcac cacgatcttg gcgtccttgt cgctgatgcg gttctcgatg
      721 ttcgactcaa ggttgccgcg ggggatgaag atcgccccgg gaatcgaccc ctgctcgaat
      781 tcgtcggcct cgcggacatc caacacgatg gcaccggcgt cgcggagttc ctccgcctcc
      841 gacgtcgaga cctcgacgat ctcacccttg gtgctgttca acaaatcacg aaacgatggc
      901 atggcagctc tctttgcgaa gtacgcgacg cccggatggg ggaacacccc cgcgagcggc
      961 acgaaatcct agtgaattgg tcaacaattg aaaaagccag ctgattcccg cgctcgacga
     1021 ccgcggccga cagccagcag ccgtcgtacc cggcgccagc agcggcaccg cccgcggcgt
     1081 gtcggcgggt agggtcgcag cgatgcagat ctacacccga aagggcgacg acggcaccac
     1141 ggggctgtat ttcggtggcc gggtgcccaa ggactctccg cgaccgcagg cctacggcga
     1201 cgtcgacgag acccaatcgg ccatcggtct ggccagggcc catgccgtgc aactgggcgt
     1261 tcacgacgtc gcggaactcg accggatcct catcggggtg cagcgcgacc tgtgggtcgt
     1321 gatggccgac ctggcgaccg gaagcgacaa ccggggcaag ctcacgcctg ggcggacctc
     1381 ggtcaccgcc gagatggtca ccgctctgga gcaactgatc gattccaccg ccacccgttt
     1441 tgaaccaccg acggaattcg tcgtcccggg aggcgacgtc gtgtcggccg cgctcgacgt
     1501 cgcccgaacg gtgtgccgtc gcgcggaacg ctccgcggtc gcaatcgacg accccggctc
     1561 gcacgcgacc ggctacctca accgcctgtc cgaccttctc tggatgctgg cgcgttggca
     1621 ggagggctcg gcgctcaccg ttcgctccat ctagtggcag cctgacaaac ccattcgtat
     1681 tcaccccttc cccaccgaca cccaccgacg cacccccaag gagcaacgtg accacgctca
     1741 agcacgcccg cacactcagc acccgcacgg cggtggtcgc acgtctcgtc ccgagcgacg
     1801 cactcgatgc cgccggcgtc gacgctgcgc tactgaagcg cctctcgttc aagggtgagg
     1861 ccgggcaatc agccaccgtc acctctgagg gcaccgtcat tcgattcgtg ggcctcggcg
     1921 catctggcga ggtcaacccc accgcaatac gcaaggcaac ggccaacgcc gtacgcgaac
     1981 tcagcggtca cgcgtcggtc gccgtggacc tgggggtgct cgctacatcg gcatcgctcg
     2041 acctcgaggc ggtcgtgcag gcagccggcg agggcgcagc actcggcgca tatcggttca
     2101 ccgagtacaa ggcggcctcc gacaccccgt acgtcgcgac ggtcaccctc gtcgccccgg
     2161 gcggcaaggc cgggctcact cgagcgattg cgatctccga ttcggtgaac tgggcccgcg
     2221 acctcgtcaa cgagccgggg ggcaccctga ccccgaccgt gttcgcccaa cgggtgaaag
     2281 aacgagcctc cgcggcgggg ctcaaggtcg aggtgctgac cgccaagcag atcgccgcca
     2341 aaggcctcgg cgggctcctg gcggtcaacc gcggctcggt cgaagagccc agattcgtca
     2401 aactcaccta caaccccgcc accgccgcgg cgcgcaagcg cccctcggtc gggctggtcg
     2461 gcaaggggat cacgttcgac tcgggcggac taagcatcaa gcccgtcgcg gggatgatga
     2521 cgatgaagtg cgacatggcc ggggccgccg ccgtcgtggc gaccatggtg gccctaccgg
     2581 ccctcggagt cggcgttccg gtcaccagct acacgccgct caccgacaat atgcccggac
     2641 ccgacgcgac gcggcccggc gacatctacc gggcccacaa cggcacgacc gttgaggtcc
     2701 tcaataccga cgccgagggt cgactcgtgc tcggcgacgc gctctcgatc gcctccgacg
     2761 acggccaccg caccatcatc gacctcgcca cgctcaccgg agcctgcatg gtggcgctcg
     2821 gcgaccagat cgccggactc atgggaaccg acgacgaatt gatcgaagca ttgaagaccg
     2881 ctgccgaacg tgccggtgag aaggtctggc acctgccctt gcccgccgag taccgcaagc
     2941 tgatcgactc cccgatcgcc gacatcaaga acatcggtgg ccaatacggg ggaacactga
     3001 ccgccgggtt gttcctcaag gagttcgtcg gcgacgacat cgcctgggcg catctcgaca
     3061 tcgccggtcc ggcgttcacc gaggcggcga acgacgagat ttcgcgcggc ggcacgggat
     3121 tcggcgtccg caccctcatc gaactgctcg aggcccgcgc tcggtagccg cgccgaggcc
     3181 gagcgacgtc gccgcccgct gcgactccac cggcccgatc accggcgggg tgaggtcacg
     3241 cgtggactca ccccgccggt ttgtagatcc cgatgaacgg cccgcactcc acgcggtcgc
     3301 cgggaagcgc ttcgtagatg tcgcggacca tgcccccctc gtagtcgaag ggggtgctgt
     3361 cggccgtcgc cgtcggcatc gccgactgat gagcgagtgc gtcgagactg aacgtctgga
     3421 acaacacgag gtaatcgccg cgacgcccgg cctcgccgac ctgcgccatg attcgctcgc
     3481 gatgtgacgg gagctcaagg atgtaccacc agtctccggg gagcggattg cgggccccga
     3541 agatggtggg caacaccgcg ttgtccggca ggatcgcgaa cccgcccgat gggtggcgac
     3601 ggcgacactc cgcggcagcc ttgacgtagg tggcggtccc cacgttggtg cggatgtagc
     3661 cgaatgcggt cgccgcgtcc gagaggtgcg agtgaagttc ggattcggga aggtcgcgat
     3721 aggtgttctc cccgcgaacc ccgctcgccc aggcgacgca cgcgaccgac agggcgatga
     3781 cgacgacggc gagcgacggc gcgaccacgg ccaggctcgg cttcgggacg actcgtacga
     3841 cgaggtcgac accgcgcacc gcgacgtagg ccgcgagcgc tccggcgaac aggttcacat
     3901 tggcgtaccc ccacgacagc gagccgcacc accccaaggc caggagtgcg gcgccggcgt
     3961 cgtcgaaacg gccacgactc gcggacgctg cgaccatggt cgcgaacgcc gcccaccaca
     4021 cggcggcacc ccaggagccg ccgagcgaaa gcgcctcgtc ccacacggac caggccatcg
     4081 ccaccagcgc cagcgctgcg agtccggcgg cggccctgtg ggctccgctg aggtgcaccg
     4141 tcaccgggag gcgggcgggg ccgacgcgaa gcacccacag cccggtcgct gcggccacgg
     4201 cgagcggacg ccacctcggc aacggatcgc ggcgacggac ctgaacgatg aaccaggcga
     4261 gcgcgatgat cggcacggcg gcgtagctct gtttgaccag cggcgcgaag ccggcgagcg
     4321 ccccgccgat cgccaccgcg ctcaccgatg accggcggac accgctgcgg agcatgagca
     4381 ggctcgccat accgaccagc aggccgtcga cggtgtgcca cgccatcagc gggaaggtat
     4441 gcgccgagat caccgcaccg gccggaatca cggcgacatc gatcgggcgc agggtcttca
     4501 ggccacgtcc cgtcgcgagc atgaccgaac ccgccacggc ggcgaacagc tccgccatgg
     4561 tgacgaatcg tgacaccggc atcagggggc cgggaaggag aaggtccacg acgtgcagca
     4621 gcggcgagcc gacgggg
//
LOCUS       DAOOMA010000150         4614 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_22491_length_4614_cov_4.031367, whole genome shotgun sequence.
ACCESSION   DAOOMA010000150 DAOOMA010000000
VERSION     DAOOMA010000150.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 4614)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4614)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4614
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22491_length_4614_cov_4.031367"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1605)
                     /locus_tag="PLP95_05355"
     CDS             complement(<1..1605)
                     /locus_tag="PLP95_05355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HPB45263.1"
                     /translation="MGDTPSGTAHTDQARPKDRPWLMRTYSGHSTAKASNELYRTNLA
                     KGQTGLSIAFDLPTQTGYDSDHVMARGEVGKVGVPVSHLGHMRTLLDGIPQGEMNTSM
                     TINATAAWLLGLYVANAEDHGVDSKVLRGTTQNDIVKEYLSRGTYIFPPEASRRLIVD
                     MFAYCNRNIPHWNPMNVCSYHLQEAGATPTQEIAYSLACAIGVLDAVRDSGQVSPDAM
                     SEVVASVSFFVNAGIRFIEETCKMRAFTQMWDRLTEERYGITDPKARRFRYGVQVNSL
                     GLTEAQPENNIQRIVLEALGVTLSKSARARSLQLPAWNEALGLPRPWDQQWSLRIMQV
                     LAYETDLLEYGDIFEGSKVVEARTNELIESAQAELDEILELGGAFEAIDEMKGRLVRS
                     HTERMRRIESGEQTVVGLNSFTETEPSPLGGEDNIMKVDPAVEQAAIDELQQWRKQRD
                     NGAVEAALDELERVARSDENIMDATIALAHAGGTTGEWAGCLREVFGEFRAPTGVAAA
                     TGTASTIPAMAQLAERLKTVAGGPPRI"
     gene            1889..2143
                     /locus_tag="PLP95_05360"
     CDS             1889..2143
                     /locus_tag="PLP95_05360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB45264.1"
                     /translation="MVSASFLAQDDLTLFLGDDLIPWLMLAVGAALAVANIAAYVRPP
                     LVDPKDPDSPRRPPAPLARVVPFAVVGVVLALWALATLLS"
     gene            complement(2160..3758)
                     /locus_tag="PLP95_05365"
     CDS             complement(2160..3758)
                     /locus_tag="PLP95_05365"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HPB45265.1"
                     /translation="MTTPNTTESPAPDDSAASTPRPVRDTIAQFAHPIVTAVLALTAA
                     ALVLGRFFVDTPMWLDEALSVNIAKLPLGDIADALRHDGHPPLYYVLLHGWMSVFGDS
                     DAAVRSLSTVISIATLPLAYLAGRRLGGRRVGVTAMLLLALSPYAFRYGSEARMYSLL
                     TLIAFAGFIAVSAALETPRPLRLVAVAVATAAGLWTQYWSLWLIATAGLLVVWVAVRS
                     HRNGNPSNARGAWMTAVAMCVGGLAFVPWLPTMLYQSAHTGTPWAPSFRITNLVVTSL
                     VEFTGGAYSEAQLGSFVLVCILLIAITGRSVSANAIELRTAINSDALVPLVLLCGTVV
                     IASAVALVNGMAFAPRYASVYLPFVILLAALGINRFANGRARDAVLIAVALLSVAGVF
                     LSNRQDRSQVGVAADAIRSRSDRGVVVVCPDQLGPSMSRQLDENQFDVVRYPDFGDPH
                     FVDWVDYAERNANNDPAAFGAAVLELAQGRPLFAVFGDGFLTLEGQCGAVVETLASQR
                     APERLISPKMEDFYEPVTVMAFAG"
     gene            complement(3877..4461)
                     /locus_tag="PLP95_05370"
     CDS             complement(3877..4461)
                     /locus_tag="PLP95_05370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB45266.1"
                     /translation="MLGDELTYLIERLRLAATLTHAIPHVGLELAPRHGQALVISHDR
                     SLSPDMTPCEFRSLVARMVSNHDIVRDLGWIGDLAAIRLGGHRVTTNGVSVAVVNPSD
                     QRRISAFATTLHADAVMDSARAMARDAIANDPDAREALRDVQLRVEHDPQLGASTVIM
                     PWAAEEAIEDLLQLTDAIAQRCWVDELVTAVSVH"
BASE COUNT          774 a         1409 c         1615 g          816 t
ORIGIN      
        1 aatgcgcggc ggcccgcccg ctacggtctt gagacgctcg gccagttgcg ccatggccgg
       61 aatcgtcgaa gcggtgccgg tggcagcggc aactccggtc ggcgcccgaa actcgccgaa
      121 gacttcgcgc agacaccctg cccactcacc ggtggtacct ccggcatggg ccaacgcaat
      181 cgtggcgtcc atgatgtttt cgtcgcttcg agcaacgcgc tcgagctcat cgagagcggc
      241 ttcgaccgcg ccgttgtctc gctgcttacg ccactgctga agttcgtcga tggccgcctg
      301 ctccaccgca gggtcgacct tcatgatgtt gtcttcgccg ccgagcggtg acggctcggt
      361 ttcggtgaaa ctgttgagcc cgaccacggt ttgttcgccc gactcgattc ggcgcatgcg
      421 ttcggtatgg ctgcgaacca gacggccctt catctcgtcg atggcctcga acgctccgcc
      481 gagttcgagg atctcgtcca gttccgcctg agcggattcg atcaactcat tggtccgggc
      541 ctcgacgacc ttggacccct cgaagatgtc cccgtactcc agcaggtcgg tttcgtacgc
      601 gagcacctgc atgatgcgaa gcgaccattg ttgatcccag gggcggggaa ggcccaaggc
      661 ttcgttccag gccggaagct gcaatgaccg ggcgcgtgcc gacttcgaca acgtcacccc
      721 cagggcctcc aacacgatgc gctggatgtt gttttccggt tgggcttcgg tgagtccgag
      781 gctgttgacc tgcacgccgt agcggaaacg acgcgccttg gggtcggtga tgccgtagcg
      841 ctcctcggtg aggcggtccc acatctgggt gaaggcccgc atcttgcagg tttcctcgat
      901 gaagcggatt ccggcattga cgaagaacga caccgacgcc acgacctcgg acatggcatc
      961 gggcgaaacc tggcccgaat cgcgcaccgc gtcgagcacg ccgatggcgc aggcaaggga
     1021 ataggcgatc tcctgggtgg gcgtcgcacc ggcctcttga aggtgatacg agcacacgtt
     1081 catggggttc cagtggggga tgttgcggtt gcagtacgca aacatgtcca cgatcaaccg
     1141 gcgcgatgcc tcgggcggga agatgtacgt gcctcgggac aggtactcct tgacgatgtc
     1201 gttctgggtg gttccgcgaa gcaccttgga atcgacaccg tggtcctcgg cgttggcgac
     1261 gtacaacccg agcagccagg cggccgttgc gttgatcgtc atcgaggtgt tcatctcgcc
     1321 ctgagggatg ccgtcgagca gcgtgcgcat gtggccgaga tgcgaaaccg gaacgccgac
     1381 cttgccgacc tcgccgcggg ccatcacgtg gtcggagtca tatccggtct gagtcgggag
     1441 gtcgaacgcg atcgacagcc cggtctgacc ctttgcgagg ttggtgcggt acagctcgtt
     1501 ggaggccttc gccgtgctgt gccctgaata ggtgcgcatc aaccaggggc gatcctttgg
     1561 ccgggcctga tcggtgtgcg cggttccgct cggtgtgtca cccatgtcga cattgtccaa
     1621 catcgatcgg tgtcgcggcg aatggtgtcg gtagacgtga gcgatcgctc atgtggggca
     1681 accgtctgca gcggtgcgaa ttacagctcc ggggcggagc gagggtcacg ttgcgccagc
     1741 acctggaaag cactggtggc gatgcgtttg gagggagaga tgtgtccccc actccatagc
     1801 cccggctgtt tgtccagtgg gggcgaatcc actggcacaa tgtgtatcga cgtctcagcg
     1861 gagaccttga gtagaaagtt ccgatttcgt ggtttcggcc tcattcctgg ctcaggacga
     1921 cctcacgctc ttcctgggtg acgacctgat tccgtggctg atgctcgcgg tgggcgcggc
     1981 gctcgccgtc gcgaacatcg cggcctatgt gcggcccccg ttggtcgatc cgaaagatcc
     2041 ggattccccg cgccgcccac ccgccccgct ggcacgggtc gtgccctttg ccgtcgtcgg
     2101 ggtggtgttg gcgctctggg cgctggcgac cttgctgagt tgacggattc gctctggggt
     2161 cagccggcga atgccatgac ggtgaccggc tcgtagaaat cttccatctt cggtgagatg
     2221 agccgctcgg gagcccgttg ggacgcgagg gtctcgacca cggcgccaca ttggccttcg
     2281 agcgtgagga agccgtcgcc gaacacggca aacagcggtc ggccctgggc cagttccaac
     2341 accgcggccc cgaacgctgc cgggtcgttg ttggcgttgc gctcggcgta gtcgacccag
     2401 tccacgaagt gcggatcacc gaaatcgggg tagcgcacga cgtcgaattg gttctcgtcg
     2461 agttggcgcg acatcgacgg cccgagttga tcgggacaca cgacgacgac cccgcgatcg
     2521 gatcgggagc ggatcgcatc cgcggcgacg ccgacctggg agcggtcctg gcggttggac
     2581 aggaacaccc cggcaacgct caggagcgcg accgcgatca acacagcgtc tcgcgcccga
     2641 ccgttggcga agcgattgat tccgagcgcg gcgaggagga tgacgaacgg gaggtacacc
     2701 gacgcgtacc gcggcgcgaa cgccatgccg ttgacgagcg cgaccgcgct ggcgatcacc
     2761 acggtgccgc acaagagcac cagcggtacc agcgcgtcgg agttgatcgc cgtgcgcaac
     2821 tcgatggcgt tggccgaaac gcttcgcccg gtgatggcga tcaggaggat gcaaacgagc
     2881 acgaagctgc caagctgcgc ctcgctgtac gcgccaccgg tgaactcgac cagcgacgtc
     2941 acgaccaggt tggtgattcg aaacgacggc gcccacgggg tgccggtgtg tgcgctctgg
     3001 tagagcatcg tcgggagcca cgggacaaac gcgaggccgc caacgcacat cgccacagcc
     3061 gtcatccagg cgccgcgtgc gttcgacggg ttgccgttgc ggtggctgcg tacggcgacc
     3121 cacacgacga ggagcccggc ggtggcgatc agccacaacg accagtactg cgtccacagc
     3181 ccggctgcgg tggcgacagc gaccgcgacg agccgcagtg gcctgggtgt ctcgagggcc
     3241 gcgctgacag cgatgaagcc ggcgaaggcg atcagggtca acagtgagta catgcgggct
     3301 tcgctgccgt atcggaacgc gtagggcgac aaggccaaca agagcatcgc ggtgacgccg
     3361 acgcgccgtc cgccgaggcg acgtccagcg aggtaggcga ggggaagtgt ggcgatcgag
     3421 atgaccgtcg acagcgaacg aaccgcggca tcgctgtctc cgaacaccga catccagccg
     3481 tgaaggagga cgtagtacag gggcggatgg ccgtcgtggc ggagtgcgtc ggcgatgtct
     3541 ccgagcggga gcttcgcgat gttgacgctg agtgcctcgt ccagccacat cggcgtgtcg
     3601 acgaaaaacc gtccgaggac gagtgcggcc gccgtcaacg ccagcacggc ggtgacgatc
     3661 gggtgggcga actgtgcgat ggtgtcgcgt acagggcggg gcgtgctcgc ggcggagtcg
     3721 tcaggagcag gagactccgt ggtgttgggt gtggtcatgg tgggcgatcg tctgtggtcg
     3781 gcgaagcggc tcgcgtgccg gtgtcggtgc gcggcgtggg gcgcgctgaa cgatagtgcc
     3841 cggcgcgtcc gctgccgaag tgcccggggg cgagggtcag tgaaccgaca cggcggtgac
     3901 caactcgtcg acccagcacc tctgggcgat ggcgtcggtg agctgcaaca agtcttcgat
     3961 cgcctcctcg gcggcccacg gcatgatgac ggtcgaggcg ccgagctgag ggtcgtgttc
     4021 gacgcgcagt tgtacgtcgc gaagtgcttc gcgagcgtcg gggtcgtttg cgatggcgtc
     4081 tcgggccatc gcccttgcgg agtccatgac cgcgtccgcg tgcaacgttg tggcgaacgc
     4141 ggagatgcgc cgctggtcgg agggattcac gacggcgacc gacacgccgt tggtggtgac
     4201 ccggtggcca ccgaggcgga tcgctgcgag gtcgccgatc cagccgaggt cccgcacgat
     4261 gtcatgattc gagaccattc gggccacgag cgagcggaac tcacacggcg tcatgtcggg
     4321 gctgagcgac cggtcgtgac tgatcacgag tgcctggccg tggcgcggtg ccagctccag
     4381 tccgacgtgg gggatcgcgt gcgtgagcgt cgctgcgagc cggagccgtt cgatgaggta
     4441 cgtcagttcg tcgccaagca tgcacaacag tatgcgttag ggcaccctaa cgtgcaagct
     4501 cctgctggtt actcgtcgcg cgatgtcgtg gtggtaatcc ctgtcagggt gtcggtggag
     4561 actctcgccg tccgtcgctg tgcagcgcga agcgcgcctc gtcacgggga tgcg
//
LOCUS       DAOOMA010000151         4606 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_22536_length_4606_cov_4.204570, whole genome shotgun sequence.
ACCESSION   DAOOMA010000151 DAOOMA010000000
VERSION     DAOOMA010000151.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 4606)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4606)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4606
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22536_length_4606_cov_4.204570"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            201..1223
                     /locus_tag="PLP95_05375"
     CDS             201..1223
                     /locus_tag="PLP95_05375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB45267.1"
                     /translation="MAILALETTPGRSPDSAVQFLIAQQCPAGGFRLNYSTTAACAND
                     AEADTDASGLALSALLAVERTPEVSASLTRGTAWLLGKQQGDGSFGGTGPAAAANTNS
                     TGIIAQFLRAAGHLQAADRAAVWITGCCTVTTATAAGTALVSQLGAIAYSPAAFAAGT
                     ADGITTQSGDQWRRSTTQAVLALGLAPYGRVDVTPIVDSPTSSTAAPTTSSSTTASST
                     TSTSSTTASSTTSTSASSTTSTSSSTTEATTTTAATTSTTSTPTTSSVPTSVLGQQVS
                     RNEPPAATSGSTFGSNRASSGTDGAGGASRLATTGSNVAGLLSLAAGTILLGTAVLSR
                     RPKVAR"
     gene            1259..2335
                     /locus_tag="PLP95_05380"
     CDS             1259..2335
                     /locus_tag="PLP95_05380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB45268.1"
                     /translation="MVTAAVTALIAALTMPLLADAAAAEPVHAQAWTDGACATAAGVT
                     VVIDFGDLGGGVHVRCAPGAVSSGFDALNKVGVEFRTAIRQPGFLCRIAGQPADDPCI
                     DTSPASAYWSYWLAPRGGAWCYSNWGAGNRVPPQGSVEGWSFSRSHTSSNVPPPGLAP
                     PALAGATAPALAASDCDPKPSAPKAPVGTDSSANATTAPNTTAKAPVTAVPNGSNGSN
                     GSINSPAAANTAPNAAPSANSAGTARGATTPPAPPGSDSGPGAQTPAMAEPNPAAEVE
                     AQVAAETTVPAEDSNGSSDPTDSTSPPDSTGSDDNDDRATGAPIELSAGTDRGSPVGV
                     AVGGSLIAAMGLVALVLRKRGRAH"
     gene            2412..3590
                     /locus_tag="PLP95_05385"
     CDS             2412..3590
                     /locus_tag="PLP95_05385"
                     /inference="COORDINATES: protein motif:HMM:NF014420.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CbiQ family ECF transporter T component"
                     /protein_id="HPB45269.1"
                     /translation="MRLRRDLHPGAWWIWALAMATATTRTTNPLLIGLTLAVVALVVS
                     ERRSDAPWAKGFGAYFAFGLVIIGIRLVFRMFLDGQHGTHILFTLPEIPLPEAAAGIR
                     IGGPVSLEGMLAALYDGLRLATMLICIGAANVLANPKRLLKSVPSALYEVGSAITVAL
                     SVAPQLVESGRRVARARRLRGEVGRRTHWVRQVAIPVMTDALDRSLALAAAMDSRGYG
                     RTAGASRGSRRVTGALMLVGLAGLCAGTYGLLDTTAFGPLTAPMLIGGVAACAAGIRA
                     SGSQVQRTRYRPDPWALPEWGVAAVGTGVAVVIVVTSSIDPNQLNPSLQPLVWPSLPP
                     IPTIAILCGALAAVIAPPPAWASRSTGFDLDDDADLGTHAGVSTPDQLAALLRPKVSR
                     "
     gene            3587..>4606
                     /locus_tag="PLP95_05390"
     CDS             3587..>4606
                     /locus_tag="PLP95_05390"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HPB45270.1"
                     /translation="MIRFDHVSVTYPDTDGPTLRDVSLTIAEGELCVVIGSTGAGKST
                     LLGAINGLVPHFTGGLLSGTVTVNGKSTRTHPPRELAEVVGVVGQDPLAGFVTDIVED
                     ELAYGMEQLAIGPDVMRKRVEETLDLLGIADLRHRALRTLSGGQQQRVAIGSVLTNHP
                     SVIVLDEPTSALDPTAAEDVLATVTRLVHDLGVTVVMAEHRLERVIHYADSALYLPGD
                     GRVVAGTPAEVLEISTIAPPIVHLGRLAGWSPLPLSVRDARRAAAPLRDRLGATAPPE
                     RRPTTAEVAPIASRPPSDATAVTMSGLTVVFGRTTAVNQVDLSLFDGEIVALMGRNGS
                     GKSTLM"
BASE COUNT          734 a         1716 c         1438 g          718 t
ORIGIN      
        1 ccttcatggc tgtcaacgta atccttctcc gtcaggaacg cgagatcctt ccctttgccg
       61 agataactgg tggcgctgta tgcctttacg aagttttggc cgatgtggag gccgagaatt
      121 ctgaaaaagg cgtaattcat tatgttagat gcaaacagga ccgttttagg agtcgtcctg
      181 cgaacggatt cggtcaggcc ttggcgatcc tggcgctgga gaccaccccc ggccgatccc
      241 ccgactccgc agttcagttc ctgatcgcgc agcaatgccc tgccggaggg tttcgcctga
      301 actactcgac cacggccgcc tgcgcgaacg acgccgaggc cgacaccgac gcgtcgggtc
      361 tggcgctttc cgcgctgctc gccgtcgaac gcacccccga ggtcagcgcc tcgctcaccc
      421 gcggcaccgc gtggctgttg ggcaagcaac aaggcgatgg atccttcggg ggcaccggac
      481 cggccgctgc cgcgaacacg aacagcaccg gaatcatcgc ccagtttctg agggcggcgg
      541 gccatcttca ggccgccgac cgggctgccg tatggatcac cggatgttgc accgtcacga
      601 ccgcgactgc tgccggcacc gcactcgtca gccaactcgg ggcgattgcc tactcgcccg
      661 cagcatttgc cgccggaacg gccgacggca tcacgaccca atccggcgac caatggcgtc
      721 gctccaccac ccaggcggtc ctggcgctcg ggctcgcacc ctacggtcgc gtcgacgtca
      781 cgccgatcgt cgactcgccc acctcgagca ccgcggctcc cacgacctcg tcgagcacca
      841 ccgcgtcgag cacgacgagc acgtccagca ccaccgcgtc gagcacgacg agcacctcgg
      901 cctcgtcgac cacctcgacc tcgtcgagca ccacggaagc aacaaccaca actgcggcga
      961 ccacctcgac cacctcgact cccacgacct catcggtgcc gaccagcgtg ctgggacaac
     1021 aggtgtcccg caacgaaccg ccggccgcga cgagcggctc aaccttcggg tcgaaccgtg
     1081 cctcctcggg caccgacgga gcaggcggcg cgagtcgact cgccaccacg gggtccaacg
     1141 tcgccgggct tctcagcctc gccgccggaa cgatcctgct gggcaccgcc gtgttgagtc
     1201 gtcgcccgaa ggtcgctcgg tgagccgccg cccatcgagg accgcgtcgg tcaccgcgat
     1261 ggtaaccgcg gcggtcaccg ccctcatcgc cgcgttgacg atgccgctcc tcgccgacgc
     1321 cgccgcggcc gaaccggttc atgcacaggc ttggaccgat ggcgcgtgcg cgacggcggc
     1381 aggcgtgacg gtcgtcatcg acttcggtga cctcggtggt ggggttcacg tgcgctgcgc
     1441 acccggagcg gtgagttccg ggttcgatgc actcaacaag gtcggcgtcg agttccgcac
     1501 cgcgatccgc caacccgggt tcctctgcag aattgccggg cagccagcgg acgacccgtg
     1561 catcgacacc tcgcctgcct cggcctactg gtcctattgg ctggcccctc gcggtggcgc
     1621 gtggtgctac agcaactggg gcgccggaaa ccgggttcca ccgcaagggt cggtggaagg
     1681 atggtccttc tcccgcagtc acacgagttc gaacgttccg ccgccgggcc tggcaccgcc
     1741 agcactcgcc ggcgcaaccg ccccggcact ggcagccagc gattgcgatc cgaagccctc
     1801 cgctccgaag gcgccggtcg ggaccgacag ctctgcaaac gcgaccaccg ccccgaacac
     1861 caccgcgaag gcaccggtca cggccgttcc caacggatcc aacggatcca acggctcaat
     1921 caactcaccc gcggcggcaa acacggctcc aaatgcagcg ccctcggcaa attccgctgg
     1981 caccgcccgc ggggcgacca cgccgccggc accgcccgga tccgattccg gtccgggcgc
     2041 ccagacccca gcaatggccg agccgaaccc tgctgccgag gtcgaggctc aggtagcggc
     2101 cgagacgacc gttcccgccg aggattcgaa cgggtcctcc gatccgaccg actccacatc
     2161 accaccggac tcgaccggca gcgacgacaa cgacgaccgc gccaccggcg caccgatcga
     2221 gttgtcggca ggcaccgatc gtggttcacc ggtcggcgtc gccgtcggcg gatcgctcat
     2281 cgcggcgatg gggctggtcg cgctcgtgtt gaggaagcgt ggcagagcgc actagccctc
     2341 caccggtttc accccccgcc gcgagtcgca ctgcatttcg caccgtggtt cgtccggcgg
     2401 ttcgcactcc ggtgcggctc cgtcgcgatc tgcaccccgg cgcctggtgg atctgggcgc
     2461 tcgcaatggc aacggccacg acccgcacga ccaacccact cctcatcggc ctcaccctcg
     2521 cggtcgtcgc actcgtggtg tccgaacgac gcagcgatgc gccgtgggcc aagggattcg
     2581 gggcctattt cgcgttcggc ctcgtcatca tcgggatccg cttggtcttt cggatgttcc
     2641 tcgacggcca acacggaacg cacatcctgt tcacccttcc cgagatcccg ttaccggagg
     2701 ctgccgccgg gattcgcatc ggaggcccgg tctccctcga aggcatgctg gccgcgctct
     2761 acgacgggct ccggctcgcc acgatgctga tctgcatcgg ggccgcgaac gtcctcgcca
     2821 atccgaaacg cctgctcaag tcggtgccga gcgcgctcta cgaggtgggc tcggcgatca
     2881 ccgttgcgct cagcgttgca ccacaactcg tcgaaagcgg ccgccgcgtc gctcgagcgc
     2941 ggcgacttcg cggcgaggtg ggtcggcgaa cccactgggt gcgccaggtc gccatcccgg
     3001 tcatgaccga tgccctcgac cgctccctcg ccctcgccgc cgccatggat tcgcgcggct
     3061 acggccgcac cgccggggcc agccgcgggt cgcgccgggt gaccggagca ctcatgttgg
     3121 tcggcctcgc ggggctgtgt gccggaacct acggcctgct cgacaccacc gcattcggcc
     3181 cactcaccgc ccccatgctc atcggcggtg tggccgcgtg tgccgccggg atccgggcca
     3241 gcggatcgca ggtccagcga acccgctatc ggcccgaccc gtgggccctc ccggagtggg
     3301 gggtcgccgc agtcggcacc ggcgtcgccg tcgtcatcgt cgtcacctcg tcgatcgatc
     3361 cgaaccagct caatccgtcc ctgcaaccac tcgtgtggcc gagcctgcct ccgataccga
     3421 cgatcgccat tctgtgcggt gcactcgccg ccgtcatcgc tccacccccc gcctgggcga
     3481 gccggtcgac cggattcgac ctcgacgacg acgccgacct tggcacccac gccggggtct
     3541 cgacccccga tcaactcgcc gccctgctac gcccgaaggt ctcgcgatga tccgcttcga
     3601 ccacgtctcg gtcacctatc ccgacaccga cggaccgaca cttcgcgacg tctcactgac
     3661 gatcgccgag ggggagctgt gcgtcgtgat cggctccacg ggagccggca agtcgaccct
     3721 gctcggagcg atcaacggcc tggtgcccca cttcaccggc ggactcttgt cgggaacggt
     3781 gaccgtgaac ggaaaaagca cccgaaccca cccgcctcgc gagctggcag aggtcgtcgg
     3841 tgtcgtcgga caggatccgc tcgcggggtt cgtcaccgac atcgttgaag acgagctcgc
     3901 ctacgggatg gaacagctcg ccatcggtcc tgacgtgatg cgtaagcggg tcgaggagac
     3961 actcgacctg ctcggaatcg cggatctgcg ccaccgtgcg ctgcgcacgc tgtcgggggg
     4021 ccagcaacag cgggtcgcga tcggctcggt gctcaccaac catccgagcg tcatcgtgtt
     4081 ggatgaaccc acatcggccc tcgatccgac ggcggccgag gatgtcctgg ccaccgtgac
     4141 ccgcctggtt catgacctcg gggtcacggt cgtcatggcg gaacaccgtc tcgaacgggt
     4201 catccactat gcggacagcg cgctgtatct ccccggcgac ggccgggtcg tcgccggcac
     4261 gccagcggag gtcctcgaga tctccacgat tgcacccccg atcgttcacc tcggccggtt
     4321 ggccggctgg tcgccgctac cgctgtcggt gcgcgacgcg cgccgagcgg ccgctccgct
     4381 gcgcgatcgc ctgggcgcca cggctccccc agaacgtcga ccgacgacgg cggaggtggc
     4441 tccgatcgca agtcgaccgc ccagcgatgc gaccgcggtg accatgtccg ggctcacggt
     4501 cgtcttcgga cggaccaccg cggtgaacca ggtcgacctg tcgctcttcg acggtgagat
     4561 cgtcgcgctg atggggcgca acggatccgg taagtcgacg ctgatg
//
LOCUS       DAOOMA010000152         4605 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_22545_length_4605_cov_3.976264, whole genome shotgun sequence.
ACCESSION   DAOOMA010000152 DAOOMA010000000
VERSION     DAOOMA010000152.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 4605)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4605)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4605
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22545_length_4605_cov_3.976264"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(9..1208)
                     /locus_tag="PLP95_05395"
     CDS             complement(9..1208)
                     /locus_tag="PLP95_05395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012226693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavin oxidoreductase"
                     /protein_id="HPB45271.1"
                     /translation="MVDHTPTPDPFAPATLGNLTLRNRIIKAATFEGVMPRGKVSEEL
                     IEFHRNVAAGGVAMTTVAYCATSMSGRVHRDTMVMSDAIVPDLTRLTDAVHAEGAKAQ
                     AQIGHAGYVANQVSNGTKTLGPSTRFAAPAMGIVKAATVEQIRGIIADYVNAARVARD
                     SGFDSVEVHMGHNYFVSSFFSPNLNKRTDAYGGSIENRARLAREILEAIRAEVGTEIA
                     LTGKLNMVDGVAKGLWINESLQIAELLQDDGHLDALQLTGGSSLLNGMYFFRGPVPMQ
                     EFVDAQPKMVGLGLKVYGPRIFPTYPFEEGFFLPEARQFRDRLTMPLILLGGINKVET
                     IHHAMDEGFEFVAMGRALLREPDLINKMQAGRDESLCIHCNKCMPTIYSGTRCVITDP
                     DPIRVAR"
     gene            1329..2627
                     /gene="tyrS"
                     /locus_tag="PLP95_05400"
     CDS             1329..2627
                     /gene="tyrS"
                     /locus_tag="PLP95_05400"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HPB45272.1"
                     /translation="MTQRSADPATPTPSEPDLDPPTAHDAAVPAEFVEAANELSVGVA
                     EVLPADGLAPRLARAASERRPLRVKLGIDPSGTELTLGHAVVLRKLRQFQDLGHTAVL
                     IVGDFTGRVGDPTGRSEVRKVQSEEQVRANAATYLDQVKRILREDRLEVRYNSEWLAT
                     MDFSEVLGYTSLMTVARLLERDDFNKRYKAGQPISLMEFMYPLMQGLDSIFIDADIEL
                     GGTDQTYNNLVGRQLQTAMKRLPQLVITVPLLRGTDGADKMGKSLGNYVAIGEAPSEQ
                     YGKIMSISDDVTPHYAELTTSWNRSELEDFARLQADDPYAAKRRVAARVVELYWGADA
                     AAKAAEEFTNRFSKKQIDAEALAAHVVVADGDSVGLIALLVAAGLATSNGDARRTLQQ
                     GGIKLDGVKLDPSCETLEVAGLDGAVLQRGKRQAVRLALD"
     gene            2704..3123
                     /gene="trxA"
                     /locus_tag="PLP95_05405"
     CDS             2704..3123
                     /gene="trxA"
                     /locus_tag="PLP95_05405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015798962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HPB45273.1"
                     /translation="MNGPTSGPDPKGTHDMATIDLTKDNFESTVTGDGTVLVDWWASW
                     CGPCRAFAPIYGEASERYPDITFAKIDTEAQQELAGAFGIQSIPTIMAFRDGILLFSQ
                     PGALPQAALEDLIGQIEALDMDAVRAEIAESTGAASS"
     gene            complement(3189..>4605)
                     /locus_tag="PLP95_05410"
     CDS             complement(3189..>4605)
                     /locus_tag="PLP95_05410"
                     /inference="COORDINATES: protein motif:HMM:TIGR00350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HPB45274.1"
                     /translation="QFRGISRADVDLAAGETFEPTNFLIVGSDTRDLDVEVDGGRSKE
                     TPDGQRADTILIARADPELGAVEMLTIPRDLWISDGKGGHRRINSAYSDGPQGLIDAI
                     DESLGIPVHHYLEVNFESFQGLVDALGGIGMYFDHPVYDERSGLNIKKKGCYTLDGSQ
                     ALAFARSRYLNYSNGVKWIADPTGDLGRATRQQVFLRRALSKVSSLGLNNVGSIKVLV
                     TLARDYLTIDDDMGLREMMDLMGRFSEFDPSSMAIHRLATDSVREGNALVEQLNERKS
                     QPVIDVFTGAAPMQTPEQLALAAVKPENVLVDVLNGTDTPGLAKQGAGALEQLDFGIG
                     NIADGEAVTETTITYAEGYEKEALLVASKIQPTPTLEVDSTLTDGRVVLTLAGTLTVP
                     GVNAPATTVPTGVMGAGSTPTTASSAGAATSSAGRGDAGATTAVAAVDGDSGGSSAAA
                     IVDEVIGLQIGDPPAGITCDP"
BASE COUNT          785 a         1571 c         1473 g          776 t
ORIGIN      
        1 ccggtggctc agcgagccac cctgatcggg tcggggtcgg tgatgacgca ccgcgtgccg
       61 ctgtagatgg tcggcatgca cttgttgcag tggatgcaca gcgattcgtc gcgccccgcc
      121 tgcatcttgt tgatcaggtc gggttcgcga agcagcgccc gccccatcgc cacgaactcg
      181 aaaccctcgt ccatcgcatg gtggatcgtc tcgaccttgt tgatgccacc gagcaggatc
      241 aacggcatcg tcaaacggtc gcggaactga cgggcctcgg gaaggaagaa cccctcctcg
      301 aacgggtacg tcggaaagat ccgcggcccg tagaccttga ggcccagacc gaccatcttc
      361 ggctgcgcgt ccacgaactc ctgcatcgga accgggccgc ggaagaaata catgccgttg
      421 agcagcgagc ttccgccggt gagttgaagc gcatcgagat ggccgtcgtc ctgaagtagc
      481 tcggcgatct gaagcgactc gttgatccag aggcctttgg ccacgccgtc gaccatgttg
      541 agtttgccgg tgagtgcaat ttcggtgccg acctcggcgc ggatggcttc gaggatctcc
      601 cgggcgagcc gggcccggtt ctcgatcgaa cccccatagg cgtcggtgcg tttgttgagg
      661 ttggggctga agaagctcga cacgaagtag ttgtggccca tgtgaacctc gaccgagtcg
      721 aatcccgaat cgcgggcgac cctcgcggcg ttcacgtagt cggcgatgat cccgcggatc
      781 tgttcgaccg tcgccgcctt cacgatgccc atggcgggcg ccgcaaatcg cgtggacggc
      841 cccagggtct tcgtaccgtt cgacacctgg ttcgcgacgt agccggcgtg gccgatctgt
      901 gcctgggcct tggccccctc ggcgtgaacc gcgtcggtga gacgcgtgag gtcgggcacg
      961 atcgcgtcgc tcatcaccat cgtgtctcgg tgcacccggc cgctcatcga cgtcgcgcag
     1021 taggcgaccg tcgtcatcgc gacgccaccg gcagcgacgt tgcggtggaa ctcgatgagt
     1081 tcctccgaca ccttgccgcg cggcatgacc ccctcaaagg tggccgcctt gatgatgcgg
     1141 ttccgaagtg tcaggttccc gagggtcgcg ggagcgaagg ggtccggcgt tggggtgtga
     1201 tcgaccatgg ccggagccta caggcgtcgc ccgtgcggtt ctgtgcgaga ccgacctggg
     1261 gtcggtggcg gcgaccgggg atcggctcac cgggctacgg cgacgccgcc ggtatcctga
     1321 agggcgccat gacgcaacga agtgccgacc ccgccacgcc aaccccctcc gaacccgacc
     1381 tcgacccgcc cacggcacac gatgctgcgg tacccgccga gttcgtcgaa gccgccaacg
     1441 aactcagcgt cggcgtcgcc gaggtgctcc ccgccgacgg cctggcccca cgcctggccc
     1501 gcgctgcgag cgagcggcgt ccgttgcggg tcaagctggg gatcgacccg tcgggaaccg
     1561 agttgaccct cggccacgcc gtcgtgttgc gcaagctccg ccagttccag gacctgggcc
     1621 acacggcggt tctgatcgtc ggcgacttca ccggtcgggt cggcgatccg acgggtcgca
     1681 gcgaggtccg caaggtgcag tccgaggagc aggtgcgcgc caacgccgcg acctatctcg
     1741 atcaggtgaa gcgcatcctt cgcgaagatc gcctcgaggt gcgctacaac tccgagtggc
     1801 tcgccactat ggatttctcc gaggtgttgg ggtacacgtc gctgatgacg gtcgcccgcc
     1861 tgctcgaacg cgacgacttc aacaagcgtt acaaagctgg ccagcccatc tcgttgatgg
     1921 agttcatgta cccgctcatg cagggcctcg actcgatctt catcgacgcc gacatcgaac
     1981 tcggcggcac cgatcagacc tacaacaacc tcgtcggccg ccaactccag acggcgatga
     2041 agcgcctgcc ccagctcgtg attacggttc cgctgctgcg cggcaccgac ggtgccgaca
     2101 agatgggaaa gtcgctcggc aactacgtcg cgatcggcga ggcaccctcg gagcagtacg
     2161 gcaagatcat gtcgatctcc gacgacgtca ccccgcacta cgcggagctc acgacgagct
     2221 ggaaccggtc cgaactcgag gacttcgccc ggcttcaggc cgacgacccc tatgccgcca
     2281 agcgccgggt cgccgcgcgt gtcgtggagc tgtactgggg tgctgacgcc gccgccaaag
     2341 ccgccgagga gttcaccaat cgattctcca aaaagcagat cgacgccgag gcgcttgccg
     2401 cccacgtcgt cgtggccgac ggcgactccg tcgggttgat cgccctcctc gtcgccgccg
     2461 ggcttgcgac gtcgaacggc gacgcgcgac ggacgctgca acaaggcggc atcaaactcg
     2521 acggggtgaa actcgacccc tcgtgcgaaa ccctggaggt cgccggcctc gacggcgcag
     2581 tgttgcagcg cggcaaacgt caggccgtcc gtctggccct cgactgaccc ctcgcaccgc
     2641 cggaccctcc cccacaagcg atgaacccac ctccgggaat acccctgggg gtttaagggt
     2701 tgcatgaacg gaccgacctc cggtcccgac ccgaaaggca ctcacgacat ggccacgatc
     2761 gacctgacca aggacaattt cgaatccacg gtgaccggcg acggcaccgt gctcgtcgac
     2821 tggtgggcgt cgtggtgcgg gccgtgccgc gcctttgccc ccatctacgg cgaggcgtcc
     2881 gagcgctacc ccgacatcac cttcgccaag atcgacaccg aggctcaaca ggaactcgcc
     2941 ggagcattcg gcatccagtc gattcccacg atcatggcgt ttcgcgacgg catcttgttg
     3001 ttcagccagc cgggggcact cccccaggcc gcgctcgagg atctgatcgg gcagatcgag
     3061 gccctcgaca tggatgcggt ccgcgctgaa atcgccgagt cgaccggcgc cgccagttcc
     3121 tgacgagccc cggcctgcga ctcgggcgct gcggctcccg accggcgctg cgaggttcgc
     3181 gagcggacct acggatcgca ggtgatcccc gccgggggat cgccgatctg gagtccgatc
     3241 acctcatcga cgatcgccgc agcgctgctc ccgccgctgt cgccgtcgac cgcggcgacc
     3301 gcggtcgtgg cgcccgcatc gccacgaccc gcagacgaag tcgcagcgcc tgcagaactc
     3361 gccgtcgtcg gggtcgatcc cgcgcccatc actccggtcg gcaccgtcgt cgccggcgca
     3421 ttcaccccgg gcacggtcag ggtgccagca agcgtgagga ccacacgccc atcggtcagc
     3481 gtggagtcga cttccagggt cggcgtcggc tggatcttcg aggccaccag caacgcctcc
     3541 ttttcgtagc cctcggcgta ggtgatcgtc gtctcggtga cggcctcgcc gtctgcgatg
     3601 ttgccgatac cgaagtcgag ctgctcaagc gctcccgcac cctgcttggc gagcccgggg
     3661 gtgtcggtgc cgttcaacac atcgacgagc acgttctcgg gcttgaccgc tgcgagcgca
     3721 agctgctcgg gggtctgcat cggtgcggcg ccggtgaaca cgtcgatcac cggctgggac
     3781 ttgcgctcgt tcagctgttc gacgagcgcg tttccttcgc gcaccgaatc ggtggcgagg
     3841 cggtgaatcg ccatgctcga cgggtcgaac tcggagaacc tgcccatgag gtccatcatc
     3901 tcgcgaaggc ccatgtcgtc gtcgatggtc aagtagtcgc gagccagcgt caccagcacc
     3961 ttgatggagc cgacgttgtt gaggccgagc gaggaaacct tcgacaacgc ccgacgcaag
     4021 aacacctgct ggcgcgtggc acgacccaaa tcccccgtgg ggtcggcgat ccatttcaca
     4081 ccgttcgaat aattcagata acgggatcgg gcgaacgcca acgcctgaga gccatccagg
     4141 gtgtagcacc ccttcttctt gatattcagc ccggaccgtt cgtcatagac cgggtgatcg
     4201 aagtacatcc cgatgccgcc cagcgcgtcg acgaggccct ggaagctctc gaagttcacc
     4261 tcgaggtagt ggtgcaccgg gattccgaga gactcatcga tcgcgtcgat aaggccctgg
     4321 gggccgtcac tgtaggcgga gttgatacgg cgatgcccgc ccttgccgtc cgagatccac
     4381 agatcgcggg gaatcgtcag catctccacg gcgccgagct ccgggtcggc ccgagcgatg
     4441 aggatcgtgt cggcccgctg accatcgggg gtctccttgg aacgcccgcc gtccacctcg
     4501 acgtcgaggt cgcgggtgtc gctgcccacg atcaggaaat tcgtcggctc gaacgtttcg
     4561 ccggccgcca ggtcgacatc ggcccgcgag ataccccgga actgc
//
LOCUS       DAOOMA010000153         4599 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_22585_length_4599_cov_4.091769, whole genome shotgun sequence.
ACCESSION   DAOOMA010000153 DAOOMA010000000
VERSION     DAOOMA010000153.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 4599)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4599)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4599
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22585_length_4599_cov_4.091769"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1111
                     /locus_tag="PLP95_05415"
     CDS             <1..1111
                     /locus_tag="PLP95_05415"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HPB45275.1"
                     /translation="ADLPQRAVSFEAMLRGEPIEAPATVDPCAPAIVAFTSGTTRDPK
                     GVIHTHETIGFETRQLNQMFPGGGPAPITGAPVGHFIGMVNAFLVPLLRERPVNLVDV
                     WDPATILRLMQERNLGVAGGATYFLTSLLDHPDFTDAHLANMPFAGLGGSTVPGAVME
                     RLERMGIAAFRSYGSTEHPSITGCLLADDAAKRLYTDGRALGGVEVRLGADGEIFSRG
                     PDCFMGYIDASMTPKVVDDQGWYRTGDVGVLDADGFLTITDRVSDVIIRGGENISAVE
                     VEELLMGLAQVAEVAVVAVPDDRLGERAAAVMRLREGASMPTIEEVRAHLSAAGLAKQ
                     KWPERLVEVEEFPRTPSGKVQKFRLRERLRDESSA"
     gene            1215..2048
                     /locus_tag="PLP95_05420"
     CDS             1215..2048
                     /locus_tag="PLP95_05420"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF014698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051213 - dioxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TauD/TfdA family dioxygenase"
                     /protein_id="HPB45276.1"
                     /translation="MTTLTTEALTETVGVEILGVDGDRLLNDPDLPEQVLSILDANGV
                     VLFRELHLDDETQVAFSKRLGRVETFRNNPELPELFRVSLDPSVNPTARYLRGTFDWH
                     IDGMTEDVPISATILSAHVIAASGGETEFSSTYQAYEDLSEEEKAEFETIRVVHTVEA
                     SQRLHTPNPTEEQVKMWRSRPSKTHPLIWTHESGRKSVVLGATADHIEGRDPEEGRQF
                     LSDLLERVTVPERVYRHHWTVGDMVIWDNTGVLHRACPYDPSAGRDMHRTTLYGNEAI
                     R"
     gene            2045..2809
                     /locus_tag="PLP95_05425"
     CDS             2045..2809
                     /locus_tag="PLP95_05425"
                     /inference="COORDINATES: protein motif:HMM:NF009466.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HPB45277.1"
                     /translation="MSRWDGPRTAIVTGGASGIGLAIAQRLAADGASVAIFDVNGEAA
                     TTAAEGIAVEGGAALGLTVDVTDRAQIDAGVAQVAERFATPTILVNNAGIEGFDRFLS
                     ISLERWNRVIEVSLTGTFQCSQAVVPGMIERGWGRIVNISSSSAQGGQQMMTHYVAAK
                     AGVIGFTKALALELGPKGITVNTIPPGFIDTPMLRASAEKGLLGATVEHHAGLTPVRR
                     AGLPQDIAAACSFLCADEASYITGQVIGVNGGRNTG"
     gene            2928..3629
                     /locus_tag="PLP95_05430"
     CDS             2928..3629
                     /locus_tag="PLP95_05430"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HPB45278.1"
                     /translation="MKTNPAVETADEPVSWRDQAVARSLDSARTRAENRVQRFIDAAI
                     ELMESNDGKDFTVQQVVERSGQSLRSFYQYFGGKHELLLALFEESIRKTADQLRANCD
                     EEADPAAKLRRFVIDYHRVCRSPAPKKRVVPSRKPVAPVLVEFAQQLLTEHPAEAARS
                     FQPLVALFDEVLDEAQAAGVIRDGLNRRRIAGVVLEAIMFNAFSATISGTPVTSTAED
                     GEALYDFVLNGLGVR"
     gene            3656..4510
                     /locus_tag="PLP95_05435"
     CDS             3656..4510
                     /locus_tag="PLP95_05435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015798154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HPB45279.1"
                     /translation="MMEFAGVSAIVTGGAGGLGAAVVARFVALGASVVLVDRDERRSL
                     EVAEAVGGTGAVGGAAVGSGPGTVSVVVGDALDDATVHEAIDAARSLGRLSVLVNLAG
                     GAVGGGSTVRGDATPHSQGSFVDTLMLNVAGTFNVARLVAAAMAHDAGTMAHDAGAAS
                     ADGTEVPERGVIINTASIAAFEGQRGQVAYAAAKAAIVGMTLPMARDLAPFGIRVCTI
                     APGPMATPVMERVLDKLEVDPAKGVVFPKRMGDPAEFALAVEMIVRNPYLNGETIRLD
                     AALRLAGD"
BASE COUNT          752 a         1454 c         1625 g          768 t
ORIGIN      
        1 ggccgacctg ccgcaacggg cagtgtcgtt cgaggcgatg ttgcggggcg aaccgatcga
       61 agcccccgcc accgtcgacc cctgcgcgcc ggccatcgtc gccttcacct ccggaacgac
      121 ccgcgacccg aaaggggtca ttcacaccca cgagacgatc gggttcgaga cccgccagct
      181 caaccagatg tttccgggcg gcgggccggc tccgatcacc ggcgctccgg tcggtcactt
      241 catcggcatg gtgaacgcgt tcctggtccc gctattgcgt gagcggcccg tgaaccttgt
      301 cgatgtctgg gatccggcga cgatcctgcg tttgatgcag gagcgcaacc tcggcgtcgc
      361 cggcggggcg acctacttcc tcaccagcct gctcgatcat cccgacttca ccgacgccca
      421 tctcgccaac atgcccttcg ccggcctcgg cggttcgacg gtgcccggag cggtgatgga
      481 acggctcgag cggatgggta tcgctgcgtt tcgttcctac ggcagcaccg aacacccgtc
      541 gatcaccggg tgtctgctgg ccgacgatgc ggccaagcgc ctctacaccg acgggcgtgc
      601 gctcggcgga gtcgaggttc gactgggtgc ggacggcgag atcttcagcc gaggccccga
      661 ctgtttcatg ggctacatcg acgcctcgat gaccccgaag gtggtcgacg atcaggggtg
      721 gtatcgcacc ggagacgtcg gtgtgctcga cgcggacggg ttcctcacca tcaccgaccg
      781 ggtgtccgat gtgatcatcc gcggcggcga gaacatcagc gccgtcgagg tcgaggagtt
      841 gttgatgggg ctcgctcagg tggccgaggt ggcagtggtg gcggtgccgg atgatcgcct
      901 cggtgagcgt gccgcggcgg tgatgcgcct gcgcgaaggg gcgtcgatgc cgacgatcga
      961 ggaggtgcgg gcgcacctga gtgccgcggg tctggcgaaa cagaagtggc cggagcggtt
     1021 ggtcgaggtg gaggagtttc cacgcacccc ctcgggcaag gtgcagaagt tccggcttcg
     1081 tgagcggttg cgcgacgagt cgtcagcgtg agcgcggcgg cgccgatgat ggcggtgctg
     1141 ggtatggaaa tgacgttcta tctttgcgat aatgacgctc tacaaccggg tgaccgaaga
     1201 atcaggggga ctgcatgacc acgttgacca ccgaagcgct caccgaaacg gtgggggtgg
     1261 agatcctcgg cgtcgatggc gatcgattgc tgaacgaccc cgatctcccc gaacaggtgc
     1321 tgtcgattct cgacgcgaac ggcgtcgtgt tgtttcgcga gctccacctc gacgacgaga
     1381 cccaggtggc gttctccaag cggttgggac gtgtggagac cttccgcaac aaccccgagc
     1441 tgcccgaact gttccgggtg tcgctcgacc cgagcgtcaa cccgacggcc cgatacctgc
     1501 gcggcacctt cgactggcac atcgacggta tgaccgaaga cgtgccgatc agcgccacca
     1561 tcttgagcgc ccatgtgatc gcggcatcgg gcggcgagac cgagttctcc agcacctacc
     1621 aggcgtatga ggatttgagc gaggaggaaa aggccgagtt cgagacgatt cgagtcgttc
     1681 acaccgtcga agcctcccag cgactccaca ccccgaaccc gaccgaagag caggtcaaga
     1741 tgtggcgctc gcgccccagc aaaacccacc cgctcatctg gacccacgag tcgggtcgca
     1801 agtcggtggt gttgggggcg acggcggatc acatcgaagg gcgtgacccc gaagaggggc
     1861 gccagttcct gagcgacctg ctcgagcggg tgaccgtgcc cgagcgggtg tatcgccacc
     1921 actggacggt cggcgacatg gtgatctggg acaacacggg cgtgttgcac cgggcgtgtc
     1981 cgtacgaccc gagcgccggg cgagacatgc accgcacgac cctgtacgga aacgaggcca
     2041 tccgatgagc cgatgggatg gcccgcgcac cgcgatcgtg accggcggtg cgtcgggcat
     2101 cggcctggcg attgcccaac gcctcgcggc cgacggcgcc tcggtggcca tcttcgacgt
     2161 gaacggtgag gccgcgacga ccgccgccga gggaatcgcg gtcgagggtg gcgccgcact
     2221 cgggctgacc gtcgatgtca ccgaccgcgc tcagatcgac gccggggtcg cacaggtcgc
     2281 cgagcgtttc gccacgccga cgatcctggt gaacaacgcc ggtatcgagg ggttcgaccg
     2341 gttcttgtcg atctcgctcg agcggtggaa ccgggtgatc gaggtgagtc tgacgggcac
     2401 gttccagtgc agtcaggcag tcgtgccggg catgatcgaa cggggctggg ggcgcatcgt
     2461 gaacatctcg tcgtccagcg cgcagggtgg ccagcagatg atgacccact acgtcgcggc
     2521 gaaggcgggg gtgatcgggt tcaccaaggc gctggcactc gagctcgggc ccaaggggat
     2581 caccgtcaac acgattcccc cggggttcat cgataccccg atgttgcggg cgagcgccga
     2641 gaaggggctg ttgggcgcga ccgtcgagca tcacgcgggc ctgaccccgg tgcgccgggc
     2701 gggcctgccg caggacatcg cggcggcgtg ctcgttcctg tgcgcggatg aggccagcta
     2761 catcaccggg caggtgatcg gggtgaacgg cgggcgcaac acgggctgag tcgggtgtgg
     2821 ggagcgccat cctccatgag atgagaacga ccgttctcat catggggtcg tcggtgtgac
     2881 acatggcggt atcgacgttc tccccagccg ccggtaggct tctcctcgtg aagacgaacc
     2941 ccgcagtcga gaccgccgac gagccggtga gctggcgcga ccaggcggtc gcacgctcgc
     3001 tcgactccgc acgaacccgg gccgagaatc gcgttcagcg gtttatcgac gccgccatcg
     3061 agctgatgga atcgaacgac ggcaaggact tcaccgttca acaggtcgtc gagcgttcag
     3121 ggcagtcgct tcgcagcttc tatcagtact tcggcggcaa gcacgagctg ttgctcgcgc
     3181 tgttcgagga atcgatccgc aagacggccg atcagcttcg tgccaattgc gacgaggagg
     3241 ccgacccagc ggccaagctg cgccgtttcg tcatcgacta tcaccgcgtc tgccgctcgc
     3301 ccgcaccgaa gaagcgggtg gtgccgtcgc gcaagccggt agcgccggtg ctggtggagt
     3361 ttgcgcagca gctgctcacc gaacaccccg cagaagcggc gcgttcgttc caaccgttgg
     3421 ttgcgctgtt cgacgaggtg ctcgacgagg ctcaggccgc cggggtgatc cgtgacggac
     3481 tgaaccggcg acgcatcgcg ggcgtggtgc tcgaagcgat catgttcaat gccttctcgg
     3541 cgaccatctc gggcacgccg gtcacctcga cggcggagga cggcgaggcg ctctacgact
     3601 ttgtgctgaa cggcctcggg gtccgctgag cggctcggcg tcggcaccgg gggcgatgat
     3661 ggaattcgcc ggcgtcagcg ccatcgtgac gggaggggcc ggaggcctcg gggcggcggt
     3721 tgttgcgcgg ttcgtcgcct tgggcgcgtc ggtggtgctc gtcgatcgcg acgagcggcg
     3781 ctccctcgag gttgctgagg ccgtcggcgg gactggtgct gtcggcggtg ccgccgttgg
     3841 gtcgggcccg ggcacggtga gcgtggtcgt cggcgacgcg ctcgatgacg cgacggtgca
     3901 cgaggcgatc gacgcggccc gctcgctcgg ccgtttgtcg gtgctggtga acttggcagg
     3961 cggcgctgtg ggaggtgggt cgacggtacg cggcgacgcc acgccgcatt cacaggggtc
     4021 cttcgtcgac accctcatgt tgaacgttgc cggaaccttc aacgtggcgc gcctggtcgc
     4081 tgctgcgatg gctcacgacg ccggcaccat ggctcacgac gccggcgccg cgtcagctga
     4141 tggcaccgag gtgccggagc gaggcgtcat catcaacacc gcgtcgatcg ccgcgttcga
     4201 gggtcaacgc ggccaggtcg cctacgccgc ggcgaaggct gcgatcgtgg ggatgaccct
     4261 gccgatggcc cgcgaccttg cgccattcgg gatacgcgtc tgcacgatcg cccccggtcc
     4321 gatggcaacc ccggtgatgg agcgggtgct cgacaaactc gaggtcgatc ccgccaaggg
     4381 cgtggtgttt cccaagcgga tgggcgaccc ggccgagttc gccctcgccg tggagatgat
     4441 cgtgcggaac ccctacctga acggcgagac cattcgtctc gacgccgcgt tgcgcctcgc
     4501 tggagattga tcctcgccac gacggaccac attcgggtac attcgggttc tcagacgaga
     4561 gaatggcatt ctcgcccggg ggtggagcca tgcggcgtt
//
LOCUS       DAOOMA010000154         4565 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_22808_length_4565_cov_4.295344, whole genome shotgun sequence.
ACCESSION   DAOOMA010000154 DAOOMA010000000
VERSION     DAOOMA010000154.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 4565)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4565)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4565
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22808_length_4565_cov_4.295344"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(31..615)
                     /locus_tag="PLP95_05440"
     CDS             complement(31..615)
                     /locus_tag="PLP95_05440"
                     /inference="COORDINATES: protein motif:HMM:NF013662.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB45280.1"
                     /translation="MNAFLLPKTPITSIDEYLATDVGGVGIQTAQRLGAAATIDVITA
                     SGLRGRGGAGFPTGRKWAGIAGQQTSRRYVVCNAAEGEPGTFKDRALLRANPYQVVEG
                     LLVAAHAVDATEAFLCLKASFEHEVEAITRAVREMQSAGICTDYSVTIVSGPDEYLFG
                     EEKAMLEVIEGKPPLPRWFPPYEHGLEKNRESIG"
     gene            756..1466
                     /locus_tag="PLP95_05445"
     CDS             756..1466
                     /locus_tag="PLP95_05445"
                     /inference="COORDINATES: protein motif:HMM:NF013209.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HPB45281.1"
                     /translation="MSTIQKQARALGDPTRHGIFMLIAEADHAVGVSELTGHFGLNHN
                     AIRQHLAHLVAAGLVIERKGRSAGRGRPPHEYLIDPSAQNRWGVVGSYEQISRLLAEV
                     ITSGLAPEEVGRRSAQHLRAPDTSVDPIADLSIAMARQGFQPEVREVPGGADVVLHSC
                     PFASTASVAREVVCSMHLGIAEGLIEGTDLRVEELVACDPDSANCVLRVRYDASTEPE
                     LRRGKLTLAGDTAPDEPG"
     gene            complement(1514..2029)
                     /locus_tag="PLP95_05450"
     CDS             complement(1514..2029)
                     /locus_tag="PLP95_05450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB45282.1"
                     /translation="MTIDNTALSTPDAHTAVRLGQRWPAIRAFSWIGAICIVAGGLVA
                     AVTRPTGFAMGSWLAAFLVLVGGVAQLGLGIGQGWIATSLPEPDLVRSELTTWNLGAA
                     LTIAGSLTKSPVLTTVGSLSVAAALLLFLRGVSVSPPELQRQRLAYRLLVWFVLLSTP
                     VGIALAWIRHG"
     gene            complement(2026..3252)
                     /locus_tag="PLP95_05455"
     CDS             complement(2026..3252)
                     /locus_tag="PLP95_05455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012224788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HPB45283.1"
                     /translation="MPVPVPAGYDLTNPDIFATGVPLEEYAWLRRTAPVFWNAQTAAE
                     SSFDDGGLWILSRLADVKAVSCARTGWSSEENTAIPRFDGKTVGYQEREVQKHMMLNM
                     DDPRHARVRGVVSRGIFSPRSVDKLHDLLGRWAEKIILDAKEKGSGDFVADIACELPL
                     QAIAELVGFPQEDRKKIFDWSNQMMAYDDPDYDVDPAVAAAEILGYATVMGEDRKANP
                     RGDIVSRLVAAADDEVLSTEEFGFFILILAVAGNETTRNAISHGMQGFFDNPDQWELY
                     KRERPTTTADEIVRWGTPVQIFQRTALEDVEIGGQLITGGQRVGLLYGSANYDEEVFD
                     DPNRFDISRNPNPHVGFGGGGVHYCPGSHLAKLEIDLIFNAIADHLPDIASAGPQRRL
                     RAGWLNGIKKMPVTYR"
     gene            3427..>4565
                     /locus_tag="PLP95_05460"
     CDS             3427..>4565
                     /locus_tag="PLP95_05460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HPB45284.1"
                     /translation="MFDLNASLESGPGYDNAEDTFGNDRAHVFHSWSAQKLINPLPVA
                     RAEGSWIYDYKGNRYLDFSSQLMNVNLGHQHPKLVAAIQEAAGRLCTIAPFHANAERS
                     EAARLIAEVAPDGLEMVFFTSGGAEANENAIRMARLHTGRHKVLAAHRSYHGATHGAI
                     TLTGDPRRWPSEPAISGVVRYWGPYPYRSSFHSQSPEQETERALAHLEELIMFEGRDN
                     VAAIILESVVGTNGILVPPPGYLAGVREICDRYGIVMIADEVMAGFGRCGEWFAVQHW
                     DVTPDLICFAKGVNSGYVPLGGVVISEAIAVTFAERAYPGGLTYSGHPLAAAAAVASI
                     NIFRDEDILGQARRLGSEVIGPELASMSERHRSIGEVRGLGVFWA"
BASE COUNT          768 a         1491 c         1504 g          802 t
ORIGIN      
        1 tttctttctc aatcgggcaa atgccaacgg ctacccgata gattcccgat ttttttccag
       61 gccgtgttcg tagggtggga accaacgggg cagcggcggc ttgccttcga tcacctcgag
      121 catcgccttc tcttcaccga agaggtattc gtcggggccg ctcacgatcg tcacgctgta
      181 gtcggtgcag atgcccgccg attgcatctc tcggaccgcg cgggtgatcg cttcgacctc
      241 gtgttcgaag gaggccttga gacagagaaa ggcctcggtt gcgtcgacgg cgtgtgcagc
      301 gacgagcagg ccctcgacga cctggtaggg gttggcccgc agcagggcgc ggtccttgaa
      361 cgtgccgggt tcgccctctg ccgcgttgca cacgacgtag cggcgtgagg tctgttggcc
      421 ggcgatcccc gcccacttgc gcccggtcgg aaagccggcg ccaccccgcc cgcgaaggcc
      481 ggacgcggtg atgacgtcga tggtggccgc ggctcccagg cgttgggcgg tttggatccc
      541 gacgcctccg acgtcggtgg cgaggtactc atcgatcgag gtgatgggcg tcttgggtaa
      601 caggaacgcg ttcatgtcac tcctcggttg tgtcggcggt atccgccggg gtgctcaagg
      661 gccacggtag gccccggccc ggcggcgcgt cgagattttc tctagtctga gacggaattt
      721 actgcgggag ctcaccgacg ccggggggac cgaccatgtc gaccattcaa aagcaagccc
      781 gagcccttgg agacccgacc cgccacggaa tcttcatgct gatcgccgag gccgaccacg
      841 ccgtcggcgt cagcgaactc accgggcact tcggcctcaa ccacaacgcc atccgccaac
      901 accttgccca cctggtggca gcgggcctgg tgatcgaacg caaaggccgt tcggccgggc
      961 ggggccgacc gccgcacgag tatctcatcg atccgtcggc gcagaaccgg tggggggtcg
     1021 tcggctccta cgagcagatc agtcgactcc tcgccgaggt catcacctcc ggcctggcgc
     1081 ccgaggaggt tgggcgacgc tcagcgcagc acctgcgggc accggacacc tcggtcgacc
     1141 cgattgccga tctgagcatc gccatggccc gtcagggttt ccagccggag gtccgcgagg
     1201 ttcccggtgg tgccgacgtc gtgttgcact cctgcccctt cgcatccacg gcgagcgttg
     1261 ctcgagaggt ggtctgttcg atgcatctcg ggatcgcaga ggggctcatc gaggggaccg
     1321 acctgcgggt ggaggaactc gtcgcttgcg atcccgacag cgcgaactgc gttctgcggg
     1381 tccgctacga cgcgtcgacc gaaccggagc ttcgccgcgg gaagttgacc ctcgcagggg
     1441 acacggctcc ggacgaaccc ggatgaaccc tgagcaacgc gcgcgggagc agcggcggcc
     1501 atacggcggg tggttatccg tgacggatcc acgccagcgc gatgccgacc ggagtggaca
     1561 acaggacgaa ccaaaccagc aatcggtacg ccaaacgctg gcgctggagt tccggcggtg
     1621 agacggagac gccccggagg aacagcagca gcgccgccgc cacgctgagc gaaccgaccg
     1681 tcgtgagcac cggcgatttc gtcaggcttc ccgcaatcgt caacgcagcg ccgaggttcc
     1741 acgtggtcaa ctcgctgcgc acgaggtcgg gttcggggag gctcgtcgcg atccacccct
     1801 ggccgatgcc caggcccaac tgcgccacgc cgccgacgag caccagaaat gcggcgagcc
     1861 atgaccccat cgcaaatccg gtcggccgcg taaccgccgc cacgaggccg ccggccacga
     1921 tgcagatggc cccgatccag gagaacgcgc gaatcgccgg ccaacgttgc cccagacgaa
     1981 ccgctgtgtg cgcgtcaggg gttgaaaggg cggtgttgtc gatcgtcacc ggtaggtgac
     2041 cggcatcttc ttgatgccgt tgagccaacc ggcgcgcagc cgtcgctgag ggccggccga
     2101 cgcgatgtcg gggaggtgat ccgcgatcgc gttgaagatg agatcgatct cgagcttcgc
     2161 aaggtgcgat ccggggcagt agtgcacgcc gccaccgccg aatccgacgt gcgggttcgg
     2221 gttgcggctg atgtcgaaac ggttcggatc gtcgaagacc tcttcgtcgt agttcgccga
     2281 accgtacagc aggccaaccc gctgaccgcc ggtgatcaac tgcccaccga tctcgacgtc
     2341 ttcgagcgct gtgcgctgga agatctgcac gggggtaccc caccgtacga tctcatcggc
     2401 cgtggtcgtc ggacgctccc gcttgtacaa ctcccactgg tcggggttgt cgaagaaccc
     2461 ctgcatgccg tggctgatgg cgttgcgggt cgtctcgttg ccggcgaccg caaggatgag
     2521 gatgaagaac ccgaactcct cggtgctgag cacctcatcg tcggcggcag ccaccagccg
     2581 cgacacgatg tcgccgcgcg ggtttgcctt tcggtcttcg cccatgaccg tcgcgtaccc
     2641 gaggatctcc gcagcggcga cggccggatc gacgtcgtag tcggggtcgt cataggccat
     2701 catctggttg gaccagtcga agatcttctt gcggtcctcc tgcggaaagc cgaccagttc
     2761 ggcgatggct tgcagcggaa gctcgcacgc gatgtccgcg acaaaatcgc ccgacccctt
     2821 ctctttggcg tcgaggatga tcttttcggc ccaccggccg agcaggtcgt ggagcttgtc
     2881 caccgaccgc ggcgaaaaga tgccccggga cacgacgccg cggacgcggg catggcgtgg
     2941 atcgtccatg ttcagcatca tgtgcttttg gacctcgcgc tcctggtagc ccacggtctt
     3001 gccgtcgaag cgaggaattg ccgtgttctc ctcgctcgac cagccggtgc gggcacacga
     3061 cacggccttc acgtctgcga ggcgcgacag gatccagagc ccaccgtcgt cgaagctgct
     3121 ctccgccgcg gtctgtgcgt tccagaacac cggcgccgtg cgtctgagcc aggcgtattc
     3181 ctccagcggc accccggttg cgaagatgtc ggggttcgtc aggtcgtaac cggcgggtac
     3241 aggtacaggc atttgcgcgc ctccctcgtc gtcctgtgga cagcgcctgt ctatcaagcg
     3301 gtctgccccg tcacaaccgg tcgttcgtca cctcgatgta gtggcacgat gtggaggctc
     3361 tcacctgtgc ccggcgcatc catcgcccag cgaccccggg cagagccgac cacgaaggga
     3421 acctcgatgt tcgatttgaa tgcctcgctc gaatccggcc ccggttacga caacgccgag
     3481 gacaccttcg gcaacgaccg ggcgcacgtg tttcattcgt ggtcggcaca aaagctcatc
     3541 aatccgttgc ccgtcgctcg ggcagagggt tcgtggatct acgactacaa gggcaacaga
     3601 tacctcgact tctcaagtca gctgatgaac gtgaatcttg gccatcagca cccgaagctg
     3661 gtcgcagcga tccaagaggc ggccgggcgt ctgtgcacca tcgccccgtt tcatgccaat
     3721 gcggagcgct cggaggctgc ccgcctcatc gccgaggtcg cccccgacgg attggagatg
     3781 gtgttcttca cctccggtgg tgcggaggcg aacgaaaatg ctatccggat ggctcggctc
     3841 cacaccggtc gccacaaggt gctggcggcg caccggagct atcacggggc aacccacggg
     3901 gcgatcaccc tcaccggcga tcctcgtcgc tggccgtcgg aaccggcgat ctccggggtg
     3961 gtgcggtact gggggccgta tccgtaccga tcctcgttcc actcccagag cccggaacag
     4021 gagaccgagc gtgcgctcgc tcacctggag gaactcatca tgttcgaggg gcgcgacaac
     4081 gtcgcggcga tcatcctcga atcggtcgtc ggcaccaacg gcattctggt cccgcccccc
     4141 ggctacctcg cgggagtgcg tgagatctgt gatcgctacg gcatcgtcat gatcgcggat
     4201 gaggtgatgg ccgggttcgg gcgatgcggc gaatggtttg ccgtgcagca ctgggacgtc
     4261 acccccgacc tcatctgctt cgccaagggg gtcaactcgg gatatgtgcc actcggcggt
     4321 gtcgtgatct ccgaggcgat cgcggtcacc ttcgccgagc gtgcctatcc cggaggactg
     4381 acctacagcg ggcatccgct ggcggcagcg gcggcggtcg cctcgatcaa catcttccgg
     4441 gacgaggaca tcttgggaca ggcgcgtcgg ctcggaagcg aggtgatcgg gccggaactc
     4501 gcgtcgatgt ctgagcggca tcggtcgatc ggcgaggtgc gcggcctcgg ggtgttctgg
     4561 gcgat
//
LOCUS       DAOOMA010000155         4563 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_22830_length_4563_cov_3.958296, whole genome shotgun sequence.
ACCESSION   DAOOMA010000155 DAOOMA010000000
VERSION     DAOOMA010000155.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 4563)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4563)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4563
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22830_length_4563_cov_3.958296"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            570..1613
                     /locus_tag="PLP95_05465"
     CDS             570..1613
                     /locus_tag="PLP95_05465"
                     /inference="COORDINATES: protein motif:HMM:NF012472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HPB45285.1"
                     /translation="MTEHSSPADEGAASSVAGHRNADPLVTLPGTDVRFRPLGLGTWA
                     WGDRYTWGMGGYDTSLTEATIAGAFSASLDGGITLLDTAEVYGKGESERIIGRLLRTG
                     DVDRSAVVLATKWFPSPRQINVKSAMRSALEASLERLGVDQVDLYQVHGPTSLRGHNV
                     IAEGLASLVADGLTKSVGVSNYSVREMMSMQAQLARRGTALASNQIEFSLLRDLPLTS
                     GMLQRCRELGIVVLAYSPIGQGRLSGKYSAQNPPPGKRGFAPHPMETVDRVVAELRSI
                     GNRHGRAPSQVALRWLIQHGTVPIPGAKNAEQAMQNAGALGWQLTSEEMEQLDRASLP
                     PRWTISNRVWQHG"
     gene            complement(1638..1805)
                     /locus_tag="PLP95_05470"
     CDS             complement(1638..1805)
                     /locus_tag="PLP95_05470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB45286.1"
                     /translation="MDENNALDVASAVVVDETELAVEVIGAKRASIEAIRAWSSAGIV
                     TLSCIGRNART"
     gene            1804..2466
                     /locus_tag="PLP95_05475"
     CDS             1804..2466
                     /locus_tag="PLP95_05475"
                     /inference="COORDINATES: protein motif:HMM:NF014600.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HPB45287.1"
                     /translation="MLAGLTGDTGSSGLLGQTGLATSGLLGQTGLGTSALGLYGTGTN
                     SPLIANALAARNASALNSIGGVSATNASAASNGYGTAAQTRLATKSEIAAIEPTLTNG
                     RLADSQLAPLGVGSHKLLPQAANGFKQLRAAAAAEGVTIGITDSYRSYDAQVDVARRK
                     GLYSQGGLAAKPGTSDHGFGLAVDLDLDSEALAWMRTNASRFGFVEDTPRESWHWKFN
                     GV"
     gene            2515..2907
                     /gene="msrB"
                     /locus_tag="PLP95_05480"
     CDS             2515..2907
                     /gene="msrB"
                     /locus_tag="PLP95_05480"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012228601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HPB45288.1"
                     /translation="MGTPPIDNDAWRDKLNDEQFRILRECGTEPAWSGELLHEKRPGV
                     FHCGACDAALFGSETKFDSGSGWPSFYDVLNNEALTLIEDRSYGMVRTEVRCANCDSH
                     LGHVFPDGPAPTGLRYCINSVSLSFDPD"
     gene            3042..4514
                     /locus_tag="PLP95_05485"
     CDS             3042..4514
                     /locus_tag="PLP95_05485"
                     /EC_number="1.11.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004096 - catalase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catalase"
                     /protein_id="HPB45289.1"
                     /translation="MTNLSQRPPLTNEAGTPVADNQNSQTAGPNGAVMLQDQHLIEKL
                     ARFNRERIPERVVHARGSGAYGTFTVTADVTKYTSADFLSEVAKQTEVFVRFSTVAGG
                     RGAPEAVRDPRGFAVKFYTAEGNYDLVGNNTPIFFLKDPLKFPDFIHSQKPDPFTNKQ
                     EAENVFDFFSHSPEATHMFTWLFGDRGIPASYRHMDGFGSHTFQWVNADGERTWVKYH
                     FKTDQGIKTLTTEEAAELAGRAPESHNTDLVEAIERGEFPTWTLKMQFMSDADAKRYR
                     INPFDVTKVWNYADFPLVEVGKLELNRNADNFFGEVEQAGFDPGSFVPGIGPSPDRML
                     QGRLFAYGDAHRYRLGINHTQLPVNAPHATTAQNYGRDGLMALGTNGGRSRNYEPNSF
                     YGPAQSNVALYAGIDSDGATGSYEWEQRETDYFTQAGDLYRLIDEDAKARLVDNIAST
                     LAQIDAADARGIIERSVSYFRAADPDYGARIEARIAELRG"
BASE COUNT          885 a         1638 c         1323 g          717 t
ORIGIN      
        1 tcaacgagct cgacgaccgg ccggtctttc gaccacgcac gagatccaca tccttcacac
       61 aacgccgttg tcgctgttgt ttcgacatgc acccagatcg ggccggtgag accggcttcg
      121 acagcgacga tggtcacgcc gggcaacccg acaagcagtg cgcacatgcg cgtagggtcc
      181 aattccacga gggctctccg acatctggct ggcgtaagac accgtcagat tcgcagagcc
      241 ctcgttcgcg tcagcggacc cctacacccc gctcaaatcc gaagagtccg tttacttcga
      301 gagatggaaa gtatgacaac atttccatct gggcagggtc gacttttgcg aactacctcc
      361 gcgccactgg gtggccagtt cgcacggaaa cataggttgt tcgcgatgcc ctggcggcgc
      421 gtcaacccca tcttccgcca cgactgcacc cacgcctccc acacgcacaa ggcgcccaag
      481 tcgtcatgtc gaacggcgag ttcagccgtt cactggcgac aggtacgggt acacgacagt
      541 cgcgcgttgc cgaggggtag ggtcacacca tgacggagca ttcctccccc gccgacgaag
      601 gtgcagccag cagcgtcgcc ggccaccgca acgccgaccc gctggtgacg ttgcccggca
      661 ccgacgttcg gtttcgacca ctcggcctgg gaacctgggc gtggggggac cgctacacat
      721 gggggatggg cggctacgac acctcgctga cggaggccac catcgccggt gccttctcgg
      781 cctccctcga cggtgggatc acccttctcg acaccgccga ggtctacggc aagggggaga
      841 gcgagcggat catcggccga ctccttcgca ccggcgacgt cgaccgctcg gccgtggtcc
      901 tcgccacgaa gtggtttccg agtccgcggc agatcaatgt caagagcgcg atgcggtccg
      961 cgcttgaggc gtcgctcgaa cgtctcggcg tcgaccaggt cgacctctac caagtgcacg
     1021 ggccgaccag ccttcgcggc cacaacgtca tcgccgaggg actggcctcg ctcgtcgccg
     1081 acggactcac caagtccgtc ggtgtctcca actattcggt tcgcgagatg atgtcgatgc
     1141 aggcgcaact ggcccgtcgc ggcacggccc tcgcatccaa ccagatcgag ttctccctcc
     1201 tgcgcgacct tcccctcacg agcgggatgc tgcagcgatg tcgcgaactc ggcatcgtcg
     1261 tgctcgccta ctcccccatc gggcaaggac gactcagcgg gaagtacagc gcgcagaacc
     1321 ctcctcccgg caaacgcgga ttcgccccac atccgatgga gaccgtcgac cgagtggtgg
     1381 ccgaactgcg ctccatcggc aaccgccacg gccgcgcccc gtcacaggtg gcgctccgct
     1441 ggctcatcca acacggcact gtccccattc ccggggcaaa gaacgccgag caggcgatgc
     1501 agaacgccgg agcgcttggc tggcaactca cgtcggagga gatggagcag ctcgatcgcg
     1561 catcgctgcc gccgaggtgg acgatctcca accgcgtctg gcagcacggc tgacgccaag
     1621 atcgtcaaac cctgccttta cgtccgggca tttcggccga tacaagacag cgtgacgatg
     1681 cccgccgaac tccaagcgcg aatcgcctcg atcgaggcgc gtttcgcccc gatcacctca
     1741 accgccaact cggtctcgtc gacgacgacg gcagatgcga cgtcgagtgc gttgttttcg
     1801 tccatgctcg ccgggctcac aggcgatacc ggctcctcgg ggctgctcgg ccagacagga
     1861 ctcgccacct cgggcctgct cggccagaca ggactcggca cctcggcgct cgggctctac
     1921 ggcacgggaa cgaactcgcc gttgatcgcc aacgcgctcg ccgcacgcaa cgcctcggcg
     1981 ctgaactcga tcggcggggt gtccgccacg aacgcctccg cagcctccaa cggatacggc
     2041 accgcagccc aaaccaggct cgcaacgaag tccgaaatcg ccgcgatcga gccgacgctc
     2101 accaacggcc gactcgccga ctcacaactg gcgccgctcg gggtgggaag ccacaagctg
     2161 ttgccgcagg ccgcgaacgg gttcaaacaa ctccgcgccg cagcagcggc cgaaggtgtc
     2221 acgatcggta tcaccgactc ctatcgcagc tacgacgctc aggtcgacgt cgcccgacgc
     2281 aagggcctct acagccaagg cgggctcgcc gcgaaaccgg ggacaagtga ccacggcttc
     2341 ggcttggccg tcgacctcga cctcgactct gaagccctcg cttggatgcg caccaacgcg
     2401 tcgcgcttcg gcttcgtcga ggacacccct cgcgagtcgt ggcactggaa attcaacggg
     2461 gtctgaccca caacgtgacc gtgtcggcgc gcccgcaccg ggcagactga ccccatggga
     2521 actcctccga tcgacaacga cgcctggcgc gacaaactca acgacgaaca gttccgcatt
     2581 cttcgcgagt gcggcaccga gccggcatgg tccggcgaac tgctccacga gaagcggccg
     2641 ggcgtctttc attgcggggc gtgtgacgcc gcgctgttcg gctccgaaac gaagttcgac
     2701 tcgggctccg gctggccgag cttctatgac gtgttgaaca acgaggccct caccctcatc
     2761 gaggatcgca gctacggcat ggtccgcacc gaggtccgct gcgccaactg cgattcacac
     2821 ctcggccacg tcttccccga cggccccgcc cccaccggtc ttcgctactg catcaactcg
     2881 gtgtcgctgt cgttcgaccc agactgatcg accggcaccc cttccttaca ctcgccgaca
     2941 cttcgatagg cgacgcctat gaggctgaga cgatcaaagt attggaatag ctctcggccc
     3001 gagacgacga tggtcgtaca ccaacacccc aggagcgaat cgtgacgaac ttgagccaac
     3061 gaccaccact gaccaacgag gccggcaccc cggttgccga caatcagaac tcccagaccg
     3121 caggccccaa cggcgcggtg atgttgcagg accaacacct gatcgagaaa ctggcccgct
     3181 tcaaccgtga gcgcatcccc gagcgagtcg tgcatgcccg cggttcgggt gcatatggca
     3241 cgttcaccgt caccgccgat gtcacgaagt acacctcagc cgacttcctc tccgaggtcg
     3301 ccaagcagac cgaagtgttc gtccgcttct cgaccgtcgc cggcggacgt ggcgcccccg
     3361 aagcggtgcg cgacccccgc ggcttcgcgg tgaagttcta cacggccgag ggcaactacg
     3421 acctcgtcgg caacaacaca ccgatcttct tcctgaagga cccgctgaag ttccccgact
     3481 tcatccactc ccaaaagccc gacccgttca ccaacaagca ggaagccgag aacgtcttcg
     3541 acttcttctc ccacagcccc gaggccaccc acatgttcac gtggctgttc ggcgaccgcg
     3601 gcatccccgc ctcgtatcgc cacatggacg gcttcggcag ccacacgttc cagtgggtga
     3661 acgcggatgg cgaacgcacc tgggtcaagt accacttcaa gaccgaccag ggcatcaaga
     3721 cgctcaccac cgaggaggcc gccgagttgg caggccgtgc ccccgagtcg cacaacaccg
     3781 acctcgtcga ggccatcgaa cgcggcgagt tcccgacatg gaccctcaag atgcagttca
     3841 tgagcgacgc cgacgcgaag cgctatcgca tcaacccctt cgacgtcacc aaggtctgga
     3901 actacgccga cttccccctc gtcgaggtcg gaaagttgga actcaaccgc aacgccgaca
     3961 acttcttcgg cgaagtggaa caagccggat tcgaccctgg ctcgttcgtg ccgggaatcg
     4021 gaccgtcccc cgaccgcatg ttgcagggcc gactgttcgc ctacggcgac gcacatcgct
     4081 accgcctcgg catcaaccac acgcaactgc cggtcaacgc accgcacgcc acgaccgcgc
     4141 agaactacgg ccgtgacggg ctgatggcgt tgggcaccaa cggtggtcgg tcgaggaact
     4201 acgagccgaa cagcttctac ggaccggcgc aaagcaacgt cgctttgtat gccgggatcg
     4261 attccgacgg agcgaccggc agctacgaat gggaacagcg cgagaccgac tacttcaccc
     4321 aggccggtga cctgtaccgg ttgatcgacg aggacgcgaa ggcccgcctc gtcgacaaca
     4381 tcgcctccac cctcgcccag atcgacgccg cggatgcccg cggaatcatc gagcggtcgg
     4441 tgtcgtactt ccgagccgcc gatcccgact acggcgcccg catcgaagct cgtatcgccg
     4501 agctccgcgg ctgatccggc ggttctcgtc aatccgggtg gctgcccgcg gtgccctcac
     4561 cgc
//
LOCUS       DAOOMA010000156         4554 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_22894_length_4554_cov_4.741498, whole genome shotgun sequence.
ACCESSION   DAOOMA010000156 DAOOMA010000000
VERSION     DAOOMA010000156.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 4554)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4554)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4554
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22894_length_4554_cov_4.741498"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1191
                     /gene="nhaA"
                     /locus_tag="PLP95_05490"
     CDS             <1..1191
                     /gene="nhaA"
                     /locus_tag="PLP95_05490"
                     /inference="COORDINATES: protein motif:HMM:NF018648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaA"
                     /protein_id="HPB45290.1"
                     /translation="WSAGYEALWSTPVRFEIGTYAFREDLGHVVNDLLMAVFFFVVGM
                     EIKRELVTGELRDRRAVALPAIAALGGMVVPATIYLAVNAGGDGSAGWGIPMATDIAF
                     ALGIVALLGRRVPGTVKVMLLTLAIVDDIGAIVVIAVFYTDRVEWNFVLAAAGCAALL
                     AAMHRGRVLYMPVIGLVGLVLWVLVYESGIHATIAGVVMGLLTPARPAQPALETERII
                     DVLEGDDDLDAEEVRAAAFAIRDSVSACDRLIEALHPWTSYVIVPIFALANAGIALSR
                     ESIGSPSRVFVGVFVALVAGKLIGISAASWLAVRFGLARLPAGATWGHVVGVGALGGI
                     GFTVSLFITGLAFDDPGLVVDAKVGVLLASITAAGVGAAIFASTSSTTSQPLVSEPRV
                     RRGN"
     gene            complement(1351..2961)
                     /locus_tag="PLP95_05495"
     CDS             complement(1351..2961)
                     /locus_tag="PLP95_05495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB45291.1"
                     /translation="MSRINDTGSLGGVGGVIAFQLRRDRWWLPAWVLSNTLVTFVLVN
                     AVKSSYATTEAIAGYSSAMGASPASRMMSGRQAALDTLGGITVSEATVISVIMVSLMA
                     LFTVMRHTRGDEEAGRTDLLRSTAVGPQAIPSAAITVSVLASLVTGALNSLVFVNGGL
                     PLAGSIGFGAGLGLTGVVFTASSAVAGQIAASARGALGLGGSFIGAMFIIRGIAAVSD
                     SWWGWLTPFRVAQEVRPFGNERWWPLGILLAAATVGFVLTATLSSRRDLGAGLRPPRP
                     GPAHARGALGTPIGLAIHEQRGLAVGWLAGLAAAAALFGAITTDITASLESNPDLAEF
                     LLASSGSGDLSDGFATFSFQILAILLTVFATAAALRLRSGESQGFAEVVLATATSRTR
                     WVAATLAATAISTVLISIGIGLSFGATYAAVDGDGSHVAASILTALSQMPAVLVMPAT
                     ALAIWAWTRSALGWLLLAFAIAPTFLGEVLKLPEWMSAISPFWHTPSFPATGSTPSIA
                     GLAVLAGVAGSFGIIGVIGINRRDIVST"
     gene            complement(2958..3851)
                     /locus_tag="PLP95_05500"
     CDS             complement(2958..3851)
                     /locus_tag="PLP95_05500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPB45292.1"
                     /translation="MTDHALELTSVTKCFGGVRALDQLDLVVPAGEVYGFLGPNGAGK
                     STAMRVLLGLLRSDSGTARLLGGDPWADAADLHRRIAFVPGDVALWPGLTGGEIIDVI
                     GRLRGGHNPATRSALIERFQLDPTKRARTYSKGNRQKVALVAAFAADADLYLLDEPTS
                     GLDPLMEVVFQECVIEANRRGATVLLSSHILAEVETLCDRIGIIRDGRMVRTATLDEL
                     RHMGRTTVSATVHSPLNLGALCGIHTPEVNGDRVTFSVDSEHLGAALAALYEAGVSSL
                     NAHPPTLEELFLAHYDTEVVQ"
     gene            4043..>4554
                     /locus_tag="PLP95_05505"
     CDS             4043..>4554
                     /locus_tag="PLP95_05505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB45293.1"
                     /translation="MVAMPSSFLHEPRPADAPPVDRLDRILAYGFAVVVLVEGVVRPD
                     VAWRVFVTVAALALSPALLLRRRSPLVSVAIGFGVAGVLSILQLATRSDELGLYSMMV
                     VPVLLYSLVRWGSGREVVVGTAFVTVVVATGMYASATGWADVFGGIVFLLLFVCLGAV
                     FRFRSALWQR"
BASE COUNT          739 a         1466 c         1521 g          828 t
ORIGIN      
        1 tggagcgctg ggtatgaagc gttgtggtcg accccggttc gattcgagat cggtacctat
       61 gcgtttcgcg aggaccttgg ccatgtggtc aacgacctgt tgatggcggt gttcttcttc
      121 gtcgtcggca tggagatcaa acgcgaactc gtcaccggcg aactgcgcga ccgacgcgcc
      181 gtcgcgctcc cggcgatcgc cgcactcggt ggaatggtgg tgcccgcgac gatctacctg
      241 gccgtcaacg ctggcggcga tggaagcgcc ggctggggaa ttccgatggc gaccgacatc
      301 gcgttcgcac tcggtatcgt cgcgttgttg ggtcggcgcg ttccgggcac ggtcaaggtg
      361 atgctgctca cactcgcaat cgtcgacgac atcggtgcaa tcgtcgtgat cgcggtgttc
      421 tacaccgatc gagtcgaatg gaacttcgtc cttgccgcag cgggttgcgc cgctttgctg
      481 gccgcgatgc accgaggtcg ggtcctgtac atgccggtga tcggccttgt cgggctggtg
      541 ttgtgggtgc tggtctacga gtccggtatt cacgccacca tcgccggagt cgtcatgggg
      601 ctgctgaccc ccgcccgtcc ggcacaaccc gcgttggaga ccgaacggat catcgacgtg
      661 ctcgaaggcg acgacgacct cgacgccgaa gaggtgcggg ctgccgcgtt cgcgatccga
      721 gattcagtct cggcatgtga ccgcttgatc gaggcgctcc acccgtggac cagttatgtg
      781 atcgtgccga tcttcgcgtt ggccaacgcc gggatcgccc tgtcccgcga atcgatcggt
      841 tcgccctcga gggtgttcgt cggtgtgttc gtcgcactgg ttgccggcaa actcatcggc
      901 atttcggcgg cgagttggct cgcggtgcgg ttcggcctcg cccggctccc cgccggagcc
      961 acttggggcc acgtcgtcgg agttggcgcg ctcggcggca tcgggttcac cgtgtcgttg
     1021 ttcatcaccg gactcgcttt cgacgatccg ggactcgtcg tcgacgccaa agtcggcgtg
     1081 ctgctggcgt cgatcacggc cgccggtgtc ggggccgcga tcttcgccag cacgtcgtca
     1141 acaacttccc agccccttgt gtccgaaccc cgcgtcagga gggggaacta gtgccctgtc
     1201 tgcggacgtt tgcacgttgc gatggccaga tgacttcgct cgctgcattt cggactcctt
     1261 gaattggggc aagaccagtc ctgcgtcgcc gcgccttgcg agcaccgccc ccggctcgcc
     1321 gcaccatcgc aaacgtttct tgccacggca ctaggtcgag acgatgtcgc ggcggttgat
     1381 cccgatgact ccgatgatcc cgaaggatcc ggcaacacca gcgaggacgg ccaacccggc
     1441 gatggacggc gtcgatccgg tcgccgggaa gcttggggtg tgccagaacg gcgagatcgc
     1501 cgacatccac tcgggaagct tcaacacctc accgaggaag gtcggagcga tcgcgaaggc
     1561 aaggagcaac caaccgagcg cggagcgggt ccacgcccag atcgccaacg cggtcgccgg
     1621 catcaccagc accgccggca tctggctcag cgcagtgagg atcgacgcgg ccacgtgcga
     1681 gccgtcgccg tcgacagcag cgtaggttgc gccgaagctc agcccgattc cgatgctgat
     1741 caacacggtg gagatggccg tggccgcgag cgtcgcagcg acccatcggg tccgggaggt
     1801 cgcggtggcg agaaccacct cggcgaagcc ctgggactcc ccagatcgga gtcgaagcgc
     1861 cgcagcggta gcgaacaccg tgagcaggat cgccaggatc tgaaacgaga acgtagcgaa
     1921 accgtcgctc agatccccgg accccgacga ggccaggagg aattcggcga ggtcggggtt
     1981 ggactcgagc gacgcggtga tatcggtcgt gatggcgccg aacagcgcgg cagccgccgc
     2041 gagcccagcc aaccagccga ctgcgaggcc gcgctgctca tgtatggcca gaccgattgg
     2101 cgttccgagc gcgccacgtg cgtgcgctgg gcccgggcgt ggaggtcgga gtccggcgcc
     2161 caagtctctt cgcgacgaca gcgtcgccgt cagaacgaag ccgaccgtgg ccgccgcaag
     2221 cagaatgccg agcggccacc aacgctcgtt cccaaacggt ctgacctcct gggccacgcg
     2281 aaacggtgtc agccaacccc accaggaatc gctcaccgcg gcgatgccac ggatgatgaa
     2341 catggcgccg atgaagctgc caccgaggcc gagagctcca cgggcactcg cagcgatctg
     2401 tccagcgacc gctgaacttg ccgtgaacac gacgccggtc aacccgagcc cggcgccgaa
     2461 accgatcgac cccgcgagtg gcagtccacc gttgacgaaa acgagcgagt tcagcgctcc
     2521 ggtcacgagc gaggcgagca cactgacggt gatcgcggca gatgggatcg cctgtggccc
     2581 gaccgcggtc gatcgcaaca gatcggtgcg accggcttcc tcgtcgcctc gcgtgtggcg
     2641 catcacggtg aacagggcca tcagcgacac catgatcacc gagatcacgg tcgcttcact
     2701 cactgtgatc ccgcccaggg tgtcgagggc ggcctgtcgt cccgacatca ttcgcgacgc
     2761 tggtgacgcg cccatcgccg acgaatagcc ggcgatcgcc tcggtcgtcg cgtacgacga
     2821 cttcaccgcg ttgaccaaca cgaaggtgac cagcgtgttg ctcaacaccc acgccggcaa
     2881 ccaccaacgg tcgcgccgca gttgaaacgc gatcacaccg cccaccccgc cgaggctgcc
     2941 ggtgtcgttg atgcggctca ttggacgacc tctgtgtcgt agtgggccag aaacagttcc
     3001 tcgagagtcg gtggatgcgc gttcaacgag ctgaccccag cctcgtacag cgctgccaac
     3061 gcggcgccga gatgttcgga atccacgctg aaggtgactc gatctccgtt gacctccggc
     3121 gtgtggatcc cgcagagcgc ccccaggttc agcggcgagt gaaccgtcgc ggacacggtg
     3181 gtccggccca tgtgccgaag ttcgtccaac gtcgcggttc gtaccatccg accgtcgcgg
     3241 atgatgccga tccggtcgca cagcgtctcg acctccgcga ggatgtggct cgataacaac
     3301 acggttgcgc cacgacggtt cgcttcgatc acgcactcct gaaagacgac ttccatgagc
     3361 gggtcgagcc ccgaggtcgg ttcgtcgagc aggtacaggt cggcatccgc tgcgaatgca
     3421 gcgaccagcg cgaccttctg acgattgcct tttgagtagg tgcgcgcgcg tttggtcgga
     3481 tcgagttgga agcgttcgat cagggcggaa cgtgtggctg ggttgtgacc gccgcgcaac
     3541 cggccgatca cgtcgatgat ctcgccgccg gtgagccccg gccacagggc gacatcgccg
     3601 ggcacgaagg cgattcttcg gtgaaggtcg gcggcgtcgg cccatggatc gccgccgagg
     3661 agtcgcgccg tgccgctgtc gcttcgcaag aggccgagca ggacccgcat cgcggttgac
     3721 ttcccggcgc cgttcggacc gagaaagccg tacacctcgc cggccggcac gaccaggtcg
     3781 agttggtcga gtgctcggac accgccgaaa cacttggtga ccgaggtcaa ttccagtgcg
     3841 tggtcagtca tgtgactccg ttctgtcggg ttcgtcgaag ccgggcagtg ccgtgggcga
     3901 ccgcagcccg tcctggacac cgtcgcgtgg gaatcggttc ggcgaatcgg tcgggaggtc
     3961 gaatttctct ccgccgttcg gccgcaagtg gctcgtactt tcggctgatg cgaggttgcc
     4021 ggtcgacccc tacgctgagg tcgtggtcgc catgccgtct tcattcctgc acgagccgcg
     4081 acccgccgat gcgccacccg tcgatcggct cgaccggatc ctggcgtacg gattcgcggt
     4141 cgtggtcctc gttgagggcg tggttcgacc ggatgtggct tggcgggtgt tcgtcacggt
     4201 ggcggcgctg gcactgagtc cggcattgct gttgcggagg cgatcgccgc tcgtctcggt
     4261 ggcgatcgga ttcggcgttg ccggcgtgct ttcgatactc cagttggcga cgcgatccga
     4321 tgagctcggc ctctactcca tgatggtggt cccggtgctg ctgtactccc tggtgcggtg
     4381 ggggtcgggt cgtgaggtcg tcgtcggaac ggcgttcgtg accgttgtgg tcgcgactgg
     4441 gatgtatgcc tcagctaccg ggtgggccga cgtcttcggt ggcatcgtct ttctgttgtt
     4501 gttcgtctgc ctcggcgcgg tgtttcgttt ccgctccgcc ttatggcaac gcca
//
LOCUS       DAOOMA010000157         4553 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_22903_length_4553_cov_4.761005, whole genome shotgun sequence.
ACCESSION   DAOOMA010000157 DAOOMA010000000
VERSION     DAOOMA010000157.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 4553)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4553)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4553
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22903_length_4553_cov_4.761005"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..595)
                     /locus_tag="PLP95_05510"
     CDS             complement(<1..595)
                     /locus_tag="PLP95_05510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB45294.1"
                     /translation="MVDTTQPIDADNHYYEALDAFTRHLDPKFKDRGVKPVNDGKRVK
                     LLMGGKVNSFIPNPTFDPIIVPGCLDPLFRGQIPEGVDPRTLMQVEPLREEYRNRDKR
                     LAVMDAQGLGAALMFPTLGCGVEEALKGDVDATMASLTAFNRWLDEDWGFAYQDRIFA
                     APMISLADPDAALAEVAYVLEHGARVVHMRPAPVPAPN"
     gene            793..1440
                     /locus_tag="PLP95_05515"
     CDS             793..1440
                     /locus_tag="PLP95_05515"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: protein motif:HMM:NF015410.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HPB45295.1"
                     /translation="MAGELAVDVSHPIRSVIPSVHGDVLAVLARTDVPLTGRGIARLT
                     DGVSDSGVKNALRALTAAGIVLVESHPPANLYRLNRRHLAASAISELATMRSRLIDAM
                     REHMATWDPAPWGAWLFGSTARGDGSVESDVDVLIVRPDTIDETDERWTDQLNRFIDD
                     VGAWTGNACAVVEYTRSEFERLPATSRRLFNGLTSDGIALTGQRLPSAAPARSAV"
     gene            1437..1583
                     /locus_tag="PLP95_05520"
     CDS             1437..1583
                     /locus_tag="PLP95_05520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB45296.1"
                     /translation="MSALSDARAHLAKARSQYQTVDIARTDAVKAVEWARKLLDGAAV
                     IVAE"
     gene            1703..3331
                     /locus_tag="PLP95_05525"
     CDS             1703..3331
                     /locus_tag="PLP95_05525"
                     /inference="COORDINATES: protein motif:HMM:NF016724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HPB45297.1"
                     /translation="MSVFTTIGRENWVGENDLAFAIRDRFAVSPGHTLIITKRVTPTW
                     FDATPEEQRAIFELVDRVKADLDAEVHPDGYNVGFNAGAAAGQTVDHLHVHVIPRFDG
                     DMDDPRGGVRHVIPSKGNYLADATLEPDTVEPPSHDLVTTQGRRMLLELLRCFANDEF
                     NRIDLLVSFVKRSGIKHLLHDLDRAVANGAQVRLLTTNYLAITDARALRILLNRSQTG
                     EGVTTPTEPARGSLEIRVFNDPSTSFHPKAYLFTSDEAAVRVGFVGSSNLSDSALTSG
                     VEWNLRTRDTSVLTSEFERLWSDGRSVVLTHEWLVGYEAFQAERAGLPAPASSSGPTA
                     STLDVDIVEDETLEDVPAPRGVQPDVLAALSATRLDGHQAGLVVMATGLGKTWLAAFD
                     VAEVNPTRTLFVAHRKEILTQARDIFAQVLPDKRSSLWVDGERDLSGDIVFASIAGLA
                     GVLPQLDPAAFDYVIVDEFHHADAPSYRRLLVRLRPQFLLGLTATPERADGADLLALC
                     SDNLVHDVGLRTGIELGLLVPFRYRGIVDVADYT"
     gene            complement(3358..4493)
                     /locus_tag="PLP95_05530"
     CDS             complement(join(3358..4226,4226..4493))
                     /locus_tag="PLP95_05530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611204.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HPB45298.1"
                     /translation="MTKKRRRHTPEQIIRKLDEGHRQLAAGKELDEVCRHLEIAESTW
                     HRWLAQYGGMKANDAKRLKELESENARLKKLLAEAELDKAMLKEIGGGKLLTPNRKRA
                     AVSRLRDRFGVSERRACRVVGQHRSTQRLDPPPISDDERQLREFLRAFSTERPRWGWR
                     RAAKAARKAGWQVNDKRIRRLWREEGLRVPQKRRKQPLRGIGAPVGAMCPIRPDALWA
                     LDFQFDVTVDGRTLKLLNIIDEFTREALAIEVDRSINADHVVGVLDRLAAERGRAPAF
                     VRFDNGPEFVAHAVADWCRFNDCDSVYIDPGSPWQNAWIESFNGRFRDELLNGRQWDS
                     LLEARVIIEDWRIDYNLNRPHTAHGELSPAEFAAAWTINQPQAA"
BASE COUNT          730 a         1574 c         1432 g          817 t
ORIGIN      
        1 cgtttggcgc gggaaccggg gccggccgca tgtgcacgac gcgggcgccg tgttcgagca
       61 cgtaggcgac ctcggcgagt gccgcgtccg gatcggcgag tgagatcatc ggggcggcga
      121 agatgcggtc ctgatacgcg aagccccagt cctcgtcgag ccagcggttg aacgcggtga
      181 ggctcgccat cgtcgcgtcg acgtcgccct tgagcgcctc ctccacgcca catccgagcg
      241 tcgggaacat gagcgccgcg ccgaggccct gcgcatccat caccgcaagg cgcttatcgc
      301 ggttgcggta ctcctcgcgg agcggctcga cctgcatcag cgttcgggga tcgacgccct
      361 cggggatctg cccacgaaac agcgggtcga ggcagccggg aacgatgatc gggtcgaagg
      421 tcggattcgg gatgaacgag ttgaccttgc cgcccatcag cagcttcacc cgcttgccgt
      481 cattgactgg cttgacgccg cggtccttga acttcgggtc gaggtgacgg gtgaacgcgt
      541 cgagcgcctc gtagtagtgg ttgtccgcgt cgatcggttg cgtggtgtcg accatcgtga
      601 atcgctcccc ctcaggattg gtgtggagtg ccaagcgacg ctccggtaac gccgctatac
      661 ctgaaagtaa agtagcactt tccactcggt tgggcgaggt tgtggaccag tacggctacc
      721 acccgcacgg cggggttgcc tacgaagccc cgcttgggca tattattgtc catatggata
      781 ttattgtgtc gattggcagg tgagcttgct gtggacgtct cccaccccat cagatccgtc
      841 atcccgtcgg tccacggcga cgtactcgcc gtgctagcgc gcaccgacgt cccacttact
      901 gggcgcggaa tcgctcgact gaccgatggg gtcagcgact ccggtgtgaa gaacgcgctt
      961 cgcgcgctca ccgccgcggg gattgttctc gtcgagtccc atccgcccgc caacctgtat
     1021 cgactcaacc gtcgccatct cgcggcctcg gccatctccg agctagcaac gatgcgttca
     1081 cgactcatcg acgccatgcg cgagcacatg gccacttggg atccggcccc ttggggagcc
     1141 tggctcttcg ggtcgacggc tcgtggagac ggctcagtcg agagcgacgt tgacgttctc
     1201 atcgttcgcc ccgacaccat cgacgagacc gacgaacgat ggactgatca gctcaaccga
     1261 ttcatcgacg atgtcggcgc ctggaccggc aacgcgtgtg ccgtcgtcga atacacacgc
     1321 agcgagttcg agcgccttcc tgcaacctcc cggcgactct tcaacggact cacatccgac
     1381 ggaatcgccc tcaccgggca gcggcttccg agcgctgccc cggcacggtc ggctgtatga
     1441 gcgccctcag cgacgcgcgg gcacacctcg cgaaggccag gtcccaatat caaaccgtcg
     1501 acatcgcgcg caccgacgct gtgaaggctg tcgagtgggc acggaagctg ttggacgggg
     1561 cggcggtcat cgtcgccgaa taggccaccg atactcagcc gccgtacagc gccgccagcg
     1621 ctgccgccaa gcgcagcggc tgccgagcgc agcggctgcc gagcgcagcg gctgccgagc
     1681 gccgcccacc tacactcgcc acgtgtcggt gttcacgacg atcgggcggg agaactgggt
     1741 cggcgagaac gacctggcgt tcgcgatccg cgatcgcttc gccgtctcgc ccgggcacac
     1801 gctcatcatc accaagcggg tgacgccgac ctggttcgac gcaacgcccg aggagcaacg
     1861 ggcgatcttc gagctcgtcg atcgggtcaa ggccgacctc gatgccgaag tccatcccga
     1921 tggctacaac gtggggttca acgccggcgc ggcggcgggt cagacggtcg atcatctcca
     1981 tgtccacgtc atcccgcgtt tcgacggcga catggacgac ccgcgcggcg gggttcgcca
     2041 cgtcatcccg tccaagggca actatctggc ggacgccacc ctcgagcccg acaccgtcga
     2101 gccgcccagc cacgacctcg tcaccacgca aggtcgtcga atgctgctgg aactgctccg
     2161 ctgtttcgcg aacgacgagt tcaaccggat cgatctgctg gtgagcttcg tcaaacgctc
     2221 agggatcaag cacctgctgc acgatctcga tcgggccgtc gccaacggcg cacaggtccg
     2281 gctcctcacc accaactacc tcgccatcac cgacgctcgc gcgctacgca tcctcctcaa
     2341 ccggtcacaa accggcgaag gcgtgacaac acctacggag cccgctcggg gttcgctcga
     2401 gatccgcgtc ttcaacgatc cctcgacaag ctttcacccc aaggcgtacc tgttcacctc
     2461 cgacgaggct gcggtccgcg tcggcttcgt cggcagcagc aacctcagcg actccgcgct
     2521 caccagcggc gtcgaatgga acctccgaac ccgcgacacc tcggtgctca cgtcggaatt
     2581 cgaacgcctg tggtctgatg gccgttcggt ggtgctcacc cacgaatggc ttgtcggcta
     2641 cgaggcgttc caggctgagc gcgccggact ccccgccccc gcttcgagtt ccggtcccac
     2701 tgcttcgacc ctggatgtcg acatcgtcga ggacgaaacc ctcgaggacg tccccgcgcc
     2761 caggggtgtg caacccgacg tgctcgccgc actcagcgcc acccgcctcg acggccacca
     2821 ggccggcctc gtcgtcatgg ccacggggct cggcaagacg tggctggcgg ccttcgacgt
     2881 cgcagaggtc aacccgacgc gaaccctgtt cgtcgcccat cgcaaggaga tcctcaccca
     2941 ggcccgcgac atcttcgccc aggtgctgcc cgacaaacga tcctcgctgt gggtcgacgg
     3001 cgaacgggac ctcagcggcg acatcgtgtt tgcctcgatc gcggggctcg ccggcgtgtt
     3061 gccccaactc gacccggccg ccttcgacta cgtgatcgtc gacgagtttc accacgccga
     3121 tgcaccgtcg tatcgacggc tgctcgtccg ccttcggccc cagttcctcc tcggcctcac
     3181 cgcgaccccc gagcgggccg acggtgcgga cctgttggcc ctgtgttcgg acaatctggt
     3241 tcacgacgtc gggttacgga ccggcatcga gctcggcctg ctggtcccgt ttcgctaccg
     3301 cggcatcgtc gacgtcgccg actacacctg agggggaccc gatgactggt ccagtcgcta
     3361 tgcggcttgg ggttggttga tggtccaggc ggcggcgaac tcggcggggc tgagctcacc
     3421 gtgggcggtg tggggccggt tcaggttgta gtcgatccgc cagtcttcga tgatcacgcg
     3481 ggcctcgagc aggctgtccc attggcggcc gttgagcagc tcgtcgcgga aacgtccgtt
     3541 gaagctctcg atccaggcgt tctgccacgg cgacccgggg tcgatgtaca cgctgtcgca
     3601 gtcgttgaag cggcaccagt ccgcgacggc gtgagcgacg aactcgggcc cgttgtcgaa
     3661 gcggacgaac gccggcgctc gaccgcgttc ggcggcgagt cggtcgagga cacccacgac
     3721 gtgatcggcg ttgatcgacc ggtcgacttc gatcgctagg gcttcgcgag tgaactcgtc
     3781 gatgatgttc aacagcttca gggtgcggcc gtcgacggtg acgtcgaact ggaagtcgag
     3841 cgcccacagc gcgtcgggtc gaatcgggca catcgcgccg accggtgcgc cgatcccacg
     3901 cagcggctgc ttgcgccgtt tctgcgggac gcgtaggccc tcctcacgcc acagacgccg
     3961 gatccgtttg tcgttcacct gccagccggc cttccgggcg gccttggctg ctcgtcgcca
     4021 tccccaccgg ggacgctcgg tcgagaacgc ccgcaggaac tcgcgcagct ggcgctcgtc
     4081 gtcgctgatc ggtggcgggt cgagacgttg agtggagcga tgctgaccga ccactcggca
     4141 ggctcgtcgt tcggagaccc cgaaccggtc acgcagcctg ctcacggctg cgcgtttgcg
     4201 gttcggggtc agaagtttcc ctccgcgatc tccttgagca tcgccttgtc cagctcggcc
     4261 tcagccagca gcttcttcaa ccgggcgttc tcgctctcga gttccttgag ccgcttggcg
     4321 tcattggcct tcatcccgcc gtactgggcg agccatcgat gccacgtcga ctccgcaatc
     4381 tcgagatggc ggcacacctc gtcgagttcc ttgcccgcag cgagttggcg gtggccctcg
     4441 tcaagcttgc ggatgatctg ctccggcgtg tgccggcggc gcttcttcgt catgtcgttg
     4501 ctcctctgtt cagagtcgca caccgactgg accatcaggc gggtctcacc tca
//
LOCUS       DAOOMA010000158         4534 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_23040_length_4534_cov_3.533378, whole genome shotgun sequence.
ACCESSION   DAOOMA010000158 DAOOMA010000000
VERSION     DAOOMA010000158.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 4534)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4534)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4534
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23040_length_4534_cov_3.533378"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1734
                     /locus_tag="PLP95_05535"
     CDS             <1..1734
                     /locus_tag="PLP95_05535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="error-prone DNA polymerase"
                     /protein_id="HPB45299.1"
                     /translation="VLNSPRHLGIHSGGMVLCDRPVNEVCPTEWARMENRSVLQWDKE
                     DCAAAGLVKFDLLGLAMLGAIHETIDLIAQHHGVEVDIAHLPQDDDVYDMICAADTIG
                     VFQIESRAQMATLPRLRPRTFYDLVVEVALIRPGPIQGGSVHPYIRRRNGDEPVTYLH
                     PLLEKSLAKTLGIPLFQEQLMQMAIDVAGFTPAEADQLRQAMGSKRSVERMERLRRRL
                     HAGMAANGVTPEVAEVIYSKLVAFANYGFPESHSVSFAHLVYVSAWLKYHYPAAFCAG
                     LLRSQPVGFYSPHSLVQDARRHGVVVRSPEVNASSWQANLEECETSTGGVALRLGLNS
                     VRHVGDELAQELDSARPFASIEDVARRGSSAEGGVKRNVMEALATAGAFGCFSAGAGA
                     DAGAGAGADAGRVRGRREDLWTAGATVAAGPGRLAGVVLGLDAPQLPLMDARQEALAD
                     LWATGVSAYGHPTVFLRDELDRRAVLSAQRLKTAQPDTNVRVAGVVTHRQRPATAGGT
                     LFINLEDETGLINVIVSSGCWQRYRAIARRAPALLVSGRLERADGVTNVIAHRLEALP
                     LAGAGGSRDFR"
     gene            1863..3257
                     /locus_tag="PLP95_05540"
     CDS             1863..3257
                     /locus_tag="PLP95_05540"
                     /inference="COORDINATES: protein motif:HMM:NF014600.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HPB45300.1"
                     /translation="MSPSPSVSPMRRRIVAAAVAVLTAVVPVIAAPGASGQSVSGTST
                     GASTGASTGASKKSTKKKTADDDIDLLRSDAETVRAEIDKTEAARTEALATLGAVTVE
                     VERARQAERVAAEAVQEAQDLVDEIDAKVAMAMRAAYTSNTVDYPIELAYLVDSNQAS
                     MRRRYMEHWAEKLDVLLDEQERARSELAVERERAADATKAVEQRQSEAQVQLTAIEEL
                     ESNQIQLVSAIEQRLDSALAEAAAIEELDKVMAQQIVAREQELHAAAVASAGADRGAP
                     AGTAAVAGNTPTKGAPVATGGPGSTAPPASAAPAPGPVASTAPPATAATPTTLPSGTS
                     SVDVVYVRGIPVARSIAANFEAMLSAASSAGLVISGSGYRDISVQIGLRAKNCGPSDF
                     EIWFKPSSQCSPPTAIPGRSMHEKGLAVDLTCDGVLIPSRTNKCFVWLAANAATYGLK
                     NLPSEPWHWSTNGN"
     gene            3377..>4534
                     /locus_tag="PLP95_05545"
     CDS             3377..>4534
                     /locus_tag="PLP95_05545"
                     /inference="COORDINATES: protein motif:HMM:NF012656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HPB45301.1"
                     /translation="MSFEVPSHIRPLREAVHAFMTERVEPAEPVLHRGGEEARALIRT
                     LQAEAKAEGLWALGHPAELGGGGLAFMDYVYVNEVQGRSEYGQIALGTYTLQDTLMLN
                     RYSEGAIRQTYLQALADARIGPSFAMTEPEVASSDPTQLKTRAVLDGDEWVITGHKWF
                     TTGAAQAAYTTVMCRTEDESVSPYAAFSMIIVPTDTPGYEIVRETPVLGLDHGHYELR
                     FNEVRVPKENLLGPRGQGFAIAQERLGPGRIFHCMRWLGQAQRAYELLCSRLNERTAF
                     GEPLAKKQLMQSHVFESYAEIQAARLLTLHAAHKIDQGDQARVEIGAIKVVGARMLHN
                     VIDRAIQAYGAAGLTDDTPLGYMYRTARFARIYDGPDEVHIHTVARRILKEY"
BASE COUNT          763 a         1439 c         1584 g          748 t
ORIGIN      
        1 gtgctcaact cgccgcggca cctcgggatt cattcgggag gcatggtgct gtgcgaccgg
       61 ccggtcaacg aggtgtgtcc aaccgaatgg gcgcgcatgg agaaccgctc ggtgctgcag
      121 tgggacaagg aggactgcgc tgcggccgga ctggtgaaat tcgacctgtt ggggctggcc
      181 atgttggggg cgatccacga gacgatcgac ctgatcgcgc aacatcacgg ggtggaggtg
      241 gacatcgccc acctgcccca ggacgacgac gtgtacgaca tgatctgtgc cgccgacacg
      301 atcggggtgt tccagatcga gtcccgtgcc cagatggcga ccctgccacg ccttcgcccg
      361 cggacgttct atgacctcgt ggtcgaggtg gcactcatcc gcccggggcc gatccagggc
      421 ggttcggttc acccttacat ccgccggcgc aacggcgacg aaccggtcac ctacctgcac
      481 ccgttattgg agaagtcgct cgccaagacc ctcgggatcc cgctgtttca agaacaactc
      541 atgcagatgg cgatcgacgt ggccgggttc acgccagccg aggccgacca gctacgccag
      601 gcgatggggt cgaagcgctc ggtcgagcgg atggaacggc tgcggcggcg cctgcacgcg
      661 gggatggcgg ccaatggggt cacccccgag gtcgccgagg tgatctactc gaagctggtg
      721 gccttcgcga actacgggtt tcccgaaagc catagcgtca gttttgctca cctggtctat
      781 gtgtcggcct ggttgaaata ccactatccg gcggcgtttt gtgccgggct gttgcggtcg
      841 cagccggtgg ggttctattc gccgcattcg ctggtccaag acgcacgccg ccatggggtg
      901 gtcgtgcggt cccccgaggt gaacgcatcg tcgtggcagg cgaacctcga ggagtgcgag
      961 acctcgaccg ggggtgtggc gcttcgtctg gggttgaact cggtgcgcca tgtcggcgac
     1021 gagttggccc aggaactcga tagcgcccgg ccgttcgcgt cgatagagga cgtggcccgg
     1081 cgcgggagtt cggctgaggg gggagtgaag cgcaacgtga tggaggccct cgccaccgcg
     1141 ggagcgttcg ggtgcttcag cgctggtgcc ggcgcggatg ctggtgcggg tgcgggtgcg
     1201 gatgccgggc gggtgagggg ccgacgcgag gacttgtgga cagcgggggc gacggttgcg
     1261 gcggggcctg gtcgactcgc cggagtggtg ctcgggttgg acgccccaca gttgccgctc
     1321 atggacgctc gacaagaggc gttggcggac ctgtgggcca ccggggtgtc ggcctatggg
     1381 caccccacgg tctttcttcg cgacgaactc gatcgacgcg cggtgttgtc cgcacagcgg
     1441 ctgaagacgg cccagcccga caccaacgtt cgtgtggccg gagtggtgac ccaccgtcag
     1501 cgaccggcca ccgccggggg gaccctgttc atcaacctcg aggacgagac cgggctcatc
     1561 aacgtcatcg tgtcgagcgg gtgctggcag cgctaccggg cgatcgctcg tcgtgccccg
     1621 gcgttgttgg taagcgggcg gctcgaacga gccgacgggg tcaccaacgt gatcgcccac
     1681 cgtctcgagg cgctgcccct cgccggtgcc ggaggttccc gggactttcg gtagcgatcc
     1741 ggccgaccga cgtgcgggcc cgtcaccacg tgggccggtt cggcgggcta gggtcacgac
     1801 atcgcgaacg acagtcgttc gaatgcctca acgtacggtc tctcaaggcc gatgctgttc
     1861 acgtgagtcc ctctccgtcc gtttccccga tgcgccgcag aatcgtggcc gccgccgtgg
     1921 ctgtgctcac cgcggtggtt ccggtcatcg ccgcgcccgg tgcgtcgggg caatcggttt
     1981 cgggaacgtc gaccggtgcg tcgaccggtg cgtcgaccgg agcgtcgaag aagtcgacga
     2041 agaagaagac cgccgacgat gacatcgacc tgctgcgttc cgacgccgaa accgttcgag
     2101 ccgagatcga caagaccgag gccgctcgta cggaggccct ggcgacgctc ggtgcggtga
     2161 ccgtcgaagt ggagcgtgcc cgccaagccg agcgggtcgc ggccgaggcg gtgcaggagg
     2221 cccaggatct cgtcgacgag atcgatgcga aggtggcgat ggcgatgcgc gctgcctaca
     2281 cctccaacac ggtcgactac ccgatcgagc tcgcctatct cgtcgactcg aaccaggcat
     2341 cgatgcggcg gcgatacatg gagcactggg ccgagaagct cgacgtgctc ctcgacgaac
     2401 aggagcgtgc ccgttcggag ttggcggtcg aacgcgagcg ggcggccgac gccaccaagg
     2461 cggtggaaca acgtcagtcc gaagctcagg tgcagctcac cgcgatcgag gaactcgagt
     2521 ccaaccagat tcagttggtc agtgcaatcg agcagcgcct cgactcggcg ctggccgaag
     2581 cggcagcgat cgaggaactc gacaaggtga tggctcaaca gatcgtcgcg cgggaacagg
     2641 aactgcacgc ggctgccgtc gcatcggccg gggcggaccg tggtgcgccg gcgggtaccg
     2701 cggccgtcgc ggggaacacc ccgacaaagg gcgccccggt ggccacgggt gggcctgggt
     2761 cgacggcacc gccagcgtcg gcggcgcccg cgcccggccc ggtggcgtcg accgccccgc
     2821 cggcgaccgc cgcaactccg accacgctgc cgtcggggac ctcgagtgtc gatgtcgtct
     2881 acgtgcgcgg catccccgta gctcgtagca tcgccgcgaa cttcgaggcg atgctgtcgg
     2941 ccgcctcgtc cgccggattg gtcatcagcg gtagcggcta tcgcgacatc agcgttcaga
     3001 tcgggctgcg ggccaagaac tgcggtccgt ccgatttcga gatctggttc aagccatcct
     3061 cacagtgcag cccaccgacg gccatccccg ggcgttcgat gcacgaaaag ggtcttgcgg
     3121 tggacctgac ctgtgacggt gttctgattc cgagtcgtac caacaagtgt ttcgtgtggc
     3181 tcgccgccaa cgctgcaacc tacggcttga agaacctgcc gtcggagccg tggcactggt
     3241 ctacaaacgg aaactgacag cccggcaacc gacaccgccc gttctccgat aaccagtggt
     3301 cgtgatcacg accactggtt atcggagaac gggctggcgg gggttcgggg cggtgggcga
     3361 caggtagcgt tcggggatgt cgttcgaggt gccttcccac atccgtccgc ttcgagaagc
     3421 ggttcacgcg ttcatgaccg agcgggttga acccgccgaa ccggtgttgc atcgcggcgg
     3481 cgaggaggcc cgagcgctga ttcgaacgct gcaggccgag gcgaaggccg agggactgtg
     3541 ggcgctcggg catccggccg aactcggcgg cggtgggctg gcgttcatgg attacgtgta
     3601 cgtcaacgag gtgcagggca ggagcgagta cggccagatc gcgctcggca cctacacgtt
     3661 gcaggacacg ctgatgctga atcgatacag cgagggcgcg atccgacaga cctacctcca
     3721 ggccctcgcc gatgcgcgga tcggtccgag tttcgccatg accgaacccg aggttgcgag
     3781 ttcggatccg acccagctca agacccgggc ggtgctcgac ggggacgaat gggtgatcac
     3841 cggccacaag tggttcacca ccggtgctgc tcaggccgcc tacaccacgg tgatgtgccg
     3901 caccgaggac gagtcggtgt cgccgtacgc agcgttttcg atgatcatcg tgccgaccga
     3961 cacccccggt tacgagatcg tccgcgagac ccccgtgctc ggcctcgacc acggccatta
     4021 cgaacttcgc ttcaacgagg tgcgtgtccc caaggagaat ctcctcggac ctcggggcca
     4081 ggggttcgcc atcgcacagg agcgcctcgg cccgggccgg atctttcatt gcatgcgttg
     4141 gttgggtcag gcgcagcggg cctatgagtt gctgtgttcc cggttgaacg agcgcacggc
     4201 cttcggagag cctctcgcga aaaagcaact catgcagtcc cacgtctttg agagctacgc
     4261 cgagatccag gccgcacgcc tgctgaccct gcacgccgcc cacaagatcg accagggcga
     4321 tcaggcccgt gtggagatcg gtgcgatcaa ggtggtcggg gcgcgcatgt tgcacaacgt
     4381 gatcgatcgg gcgatccagg cctacggcgc ggcggggctc accgacgaca ccccgctcgg
     4441 ctacatgtac cgcacggcgc ggttcgcgcg gatctatgac ggccccgacg aggtgcacat
     4501 ccacaccgtc gcccgacgga tcctcaagga gtac
//
LOCUS       DAOOMA010000159         4519 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_23141_length_4519_cov_3.870296, whole genome shotgun sequence.
ACCESSION   DAOOMA010000159 DAOOMA010000000
VERSION     DAOOMA010000159.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 4519)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4519)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4519
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23141_length_4519_cov_3.870296"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            106..1401
                     /locus_tag="PLP95_05550"
     CDS             106..1401
                     /locus_tag="PLP95_05550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB45302.1"
                     /translation="MTIDSPEGDSPDSANTGPSENKRPEATARTDKRPRGAADRVVRT
                     HRQTRPTVAITVFAMAAAFGAWALGGSSWVAIHLMAAVGLVTIISGVSLMLTVTWSAA
                     PAPPDAAVLVQRFCIAIGAVAVIAGRHFDVGAPVVVPGAALYLTGLALLAALLALTVR
                     RGVERRYDPAVIAYVAALCAGCAGVVVGTVMALDGSTPARVDTHITLNVLGLVGLVVV
                     GTLPFFTATVGRSRMAPKATASRIVTVVAWQSVAVAVISTAMSTQRAPVAAAGLAAYG
                     VGLVAVLALLPRPTRRQLQWAGPRLIAIWAGTLWWAVAVVASAVLVGSDGHATFSPQW
                     VWTLVVAGYGQILWGSLAYLMAVLRGGGHRLLAEGFAATRSWLGLVAANLTGVALATS
                     QPKIAAATTTVWLADTAWRAARVGFGRRALTGEHGNQER"
     gene            complement(1369..1968)
                     /locus_tag="PLP95_05555"
     CDS             complement(1369..1968)
                     /locus_tag="PLP95_05555"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HPB45303.1"
                     /translation="MSENRWLQMIEQNPGHSEWYIERFRAMAAAGDDLDGEARLVDAM
                     VPRGSRILDAGCGPGRVGGRLAALGHSVVGVDIDPALIAAAQGDHPAATWVSADLAEF
                     DLEAMGLPHEFDLAVCAGNVMTFLDPTTRRDVLVRLHSHLAEGGRCVIGFGADRGYEF
                     EEFRADAAAAGFDEELLLSAWDVRRFDASSTFLVAVLAR"
     gene            2120..3028
                     /locus_tag="PLP95_05560"
     CDS             2120..3028
                     /locus_tag="PLP95_05560"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HPB45304.1"
                     /translation="MKALITGASGFVGPHLAAHLEASGDEVVCTDRASGADVCDEAQI
                     ADVFATHRPEVVFHLAGDSDVGGSWHHPRSTFLANAVGTLNVMEAARAAGVRRVVAIG
                     SADVYGKVAVDELPLTEHSALRPASPYAASKIAADAVTQQAWLGHGLETIRVRAFNHL
                     GPGQSDRFVAPAIATRIARNELDAATSVPVGNLEARRDITDVRDVVRAYRLLAVSGEP
                     GEVYNVCSGTTVAIAELAEQLVAMARHPMELVVDESLLRPVDIPVLCGDNTKLCERTG
                     WEPELSLTTTLSDLMDDCRARLAASV"
     gene            3116..4096
                     /gene="gmd"
                     /locus_tag="PLP95_05565"
     CDS             3116..4096
                     /gene="gmd"
                     /locus_tag="PLP95_05565"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012222881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008446 - GDP-mannose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HPB45305.1"
                     /translation="MTKRALITGITGQDGSYLAEFLLDQGYEVFGMVRRSSTVTFERI
                     AHLQDDVTMVYADLLDQASMIEVLNKYRPDEVYNLAAQSFVPTSFSQPVLTGEVTALG
                     VTRLLDAIRLVNPDIRFYQASSSEMFGKVVEVPQTESTPFYPRSPYGVAKLYGHWITI
                     NYRESYDLHASSGILFNHESPRRGLEFVTRKISYGVAKIKLGRQSELRLGNLDAQRDW
                     GFAGDYVKAMWLMMQQDTADDYVVSTGETHEVREFCQIAFGHAGLNWEDHVVVDPEFF
                     RPAEVDLLIGDPTKARTKLGWEPETSFEQLVTMMVDADIAFLEGKLKGIS"
     gene            4118..>4519
                     /locus_tag="PLP95_05570"
     CDS             4118..>4519
                     /locus_tag="PLP95_05570"
                     /inference="COORDINATES: protein motif:HMM:NF014042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-phospho-L-lactate transferase CofD family
                     protein"
                     /protein_id="HPB45306.1"
                     /translation="MQITTIAGGVGAARMLRALGATWGPSSLTAIVNVADDELVNGLP
                     VSPDIDSILYTLAGANDEQRGWGLGGETWQAMSMLRRYAEANGRSDIGWFNLGDLDLG
                     SHMYRAVRLAEGATLDVVTAEMARAWGLELRI"
BASE COUNT          721 a         1605 c         1470 g          723 t
ORIGIN      
        1 gtgccgccat ctcccggttg ccgattcacc agccaacccc ctagcgtttt tccatcacct
       61 ggtagtgaaa ctatcaccgg tggaatcgag accgtcgggg gtgccatgac gatcgactcg
      121 cccgaaggcg actcaccaga cagcgcaaac actggcccga gcgagaacaa gcggcccgag
      181 gcgaccgcac gcaccgacaa gcgaccccgc ggtgcggccg accgcgtggt gcgaactcat
      241 cgccaaaccc gcccgacggt ggcgatcacg gtgttcgcca tggcggccgc gttcggcgcc
      301 tgggcactcg gcgggtcgtc gtgggtggcg atccacctca tggcggcggt cggactcgtc
      361 acgatcatca gcggcgtctc gctcatgttg acggtcacct ggtcggcggc tcccgctccg
      421 cccgatgccg cggttctggt gcagcggttc tgcattgcga tcggagcggt agcggtcatc
      481 gccgggcgcc atttcgacgt cggagccccc gttgttgtcc ccggtgccgc tctgtatctc
      541 acggggttgg cgctcctggc cgcgttgttg gcattgaccg tgcggcgcgg cgtcgagcgg
      601 cgctacgacc cggcggtcat cgcgtacgtc gccgcactgt gcgcgggatg cgccggtgtc
      661 gtggtcggca ccgtcatggc cctcgacggg tcgacgcccg cacgggtcga cacccacatc
      721 accctcaacg ttctgggcct cgtcggcctc gtggtggttg ggaccttgcc gtttttcacc
      781 gccacggtcg gacgcagccg gatggcaccg aaggccaccg cgtcgcgcat cgtgacggtc
      841 gtcgcgtggc aaagcgtcgc ggtcgccgtc atcagtaccg cgatgtcgac tcagcgggcc
      901 ccggtcgcgg ccgctggcct cgccgcctac ggcgtaggcc tcgtcgcggt tctcgcgttg
      961 ttgccgcgcc ccactcgacg gcagttgcaa tgggccgggc cgcgcctcat cgcgatctgg
     1021 gcgggcaccc tgtggtgggc ggtcgcggtg gtggcctccg cggtgctcgt gggtagcgac
     1081 ggacacgcca cgttcagtcc gcaatgggtg tggacgctcg tcgtggccgg atacggccag
     1141 atcctgtggg ggtcgctcgc ctacctcatg gcggtgcttc gcggtggcgg acaccggctt
     1201 ctcgccgagg ggtttgccgc gacgcggtcg tggttgggtt tggtggcggc gaacctcacc
     1261 ggcgtcgcac tcgcgacctc gcagccgaag atcgcggccg cgacgaccac ggtgtggctc
     1321 gccgacaccg cgtggcgcgc cgcccgagtc ggtttcggcc gccgggcgct aacgggcgag
     1381 cacggcaacc aggaacgttg aggacgcatc gaaccggcgg acatcccacg ccgacaggag
     1441 caactcctca tcaaagccag cggccgcagc gtcggcacgg aactcctcga actcgtaccc
     1501 gcgatccgcg ccgaacccga tgacgcagcg accaccctcg gcgaggtgcg aatgcaatcg
     1561 caccagcaca tcgcgccggg tcgtcggatc gaggaaggtc atcacatttc cggcgcacac
     1621 ggccaggtcg aactcgtgtg gaagccccat cgcctcgagg tcgaactccg cgaggtcggc
     1681 ggacacccag gtcgcggcgg ggtgatctcc ctgcgcggcc gcgatgagcg ccgggtcgat
     1741 gtcgacgccg acgaccgaat ggccgagggc ggcgaggcgg ccaccgaccc ggcccggccc
     1801 acagccggca tcgagaatcc gcgaaccgcg cggcaccatc gcgtcgacga gtctcgcctc
     1861 gccgtcgagg tcgtcacccg ctgcggccat cgcccggaac cgttcgatgt accactccga
     1921 atggccgggg ttctgctcga tcatctgcag ccaacggttc tcgctcatgg tctatgaccg
     1981 taccaaccgg gtcggcccgc aacgaccggc cccggaaccg gcaccgaccg ccaccgggta
     2041 gactgccgac gatgttcgcg cacgaaccga cccgtcgttg cggcccgcga tgtgccgata
     2101 cccggggtcg cgcgctccgg tgaaggctct gatcaccggc gcctccggtt tcgtcggacc
     2161 gcacctcgcc gcacacctcg aagcctccgg cgacgaagtg gtctgcaccg accgtgcaag
     2221 tggggccgac gtgtgtgacg aggcccagat cgccgacgtg ttcgcgaccc accggcccga
     2281 ggtcgtgttc cacctcgccg gagacagcga cgtcggcgga agctggcacc acccacgctc
     2341 cacgttcctc gcgaacgccg tcggaacgct caacgtgatg gaagcggctc gggccgccgg
     2401 cgttcggcgg gttgtcgcca tcggcagcgc cgacgtgtac ggcaaggtgg ccgtcgacga
     2461 gttgccgctc accgaacact cggcgttgcg tccggcaagt ccctatgcgg cctcgaagat
     2521 cgcggccgac gccgtcaccc agcaggcgtg gctcggacac gggctcgaaa cgatccgcgt
     2581 gcgggcgttc aaccatctcg gtccggggca gagcgaccgg ttcgtcgccc cggcgatcgc
     2641 cacccgtatc gcccgaaacg aactcgacgc cgcaacctcg gtgccggtcg gcaacctcga
     2701 ggcccgccga gacatcaccg acgtccgcga cgtggttcgc gcctaccgcc tcctcgccgt
     2761 gagcggcgaa cccggcgagg tctacaacgt ctgcagcggc accacggtcg cgatcgccga
     2821 actcgccgaa caactcgtcg ccatggcccg ccaccccatg gagctcgtgg tggacgaatc
     2881 gctgcttcgc ccggtcgaca tccccgtgct gtgcggcgat aacaccaaat tgtgcgagcg
     2941 caccggctgg gagccggagc tctcactcac caccaccttg tccgacctca tggacgactg
     3001 ccgcgcccga ctcgcggcct cggtgtgatc ccccggtgcg gggccccgac ggatccgtgg
     3061 atcggcccgc catcaccact actgtcaaga atccgctgat cgggagttcc cagcaatgac
     3121 caagcgcgcg cttatcaccg gtatcacggg ccaggacggc tcctatctcg ccgagttcct
     3181 gctcgaccag ggctatgagg tcttcggcat ggtccggcgc tcctcgacgg tgaccttcga
     3241 gcgcatcgcc caccttcaag acgatgtgac gatggtgtat gcggacctgc ttgatcaggc
     3301 gtcgatgatc gaggtcctca acaagtaccg gcccgatgag gtgtacaacc tcgcggcgca
     3361 gagcttcgtg cccaccagct tcagccagcc ggtcctcacc ggtgaggtca ccgccctcgg
     3421 cgtgacccgc ctcctcgacg ccatccgtct ggtgaacccc gacatccgct tctaccaggc
     3481 ctcaagttcg gagatgttcg gcaaggtcgt cgaggtccct cagaccgagt ccaccccgtt
     3541 ctacccgcgc agcccttacg gcgtggcgaa gctgtacggc cactggatca cgatcaacta
     3601 ccgcgagtcc tacgacctgc atgccagctc cggcatcctg ttcaaccacg agtcgccgcg
     3661 ccgcggcctt gagttcgtga cccggaagat ctcctacggg gtggccaaaa tcaagctcgg
     3721 gcgccagtcc gaactgcgcc tcggcaacct cgacgcccaa cgcgactggg gcttcgctgg
     3781 cgactacgtc aaggcgatgt ggctcatgat gcaacaggac accgccgatg actacgtggt
     3841 ctcgaccggc gaaacgcacg aggtgcgcga gttctgccag atcgcttttg gccatgccgg
     3901 gctgaactgg gaggaccacg tcgtggtcga cccggagttc ttccgccccg cggaggtcga
     3961 cctgctgatc ggggatccga ccaaggcccg aaccaagctc ggctgggaac ccgagacgag
     4021 tttcgaacag ctcgtgacga tgatggtcga cgccgacatc gccttcctcg agggcaagct
     4081 caaaggcatc agctgaggcc ggagcctcac catcaccatg cagatcacca cgatcgccgg
     4141 gggggtcgga gcggcgcgca tgttgcgtgc cctcggcgca acctggggac cctcgtcgct
     4201 gaccgcgatc gtcaacgtcg ccgacgacga gcttgtcaac gggcttcccg tctcccccga
     4261 catcgactcg atcctctaca ccctcgccgg ggcgaacgac gagcagcgcg gctgggggct
     4321 cggcggcgaa acctggcagg cgatgtcgat gcttcgccgc tacgcggagg caaacgggcg
     4381 cagcgatatc ggctggttca atctcggaga tctcgacctc ggcagccaca tgtatcgggc
     4441 ggtccgcctc gctgaggggg cgaccctcga cgtcgtcacc gcggagatgg cccgggcgtg
     4501 ggggctcgag ctgcgaatc
//
LOCUS       DAOOMA010000160         4515 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_23166_length_4515_cov_4.415022, whole genome shotgun sequence.
ACCESSION   DAOOMA010000160 DAOOMA010000000
VERSION     DAOOMA010000160.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 4515)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4515)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4515
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23166_length_4515_cov_4.415022"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..854
                     /locus_tag="PLP95_05575"
     CDS             <1..854
                     /locus_tag="PLP95_05575"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HPB45307.1"
                     /translation="SLIMMETFHPTETAEVLSRENVTLAGSGTPFHMAYLAAQKASST
                     PIFPNVRAFPGGGAPKPPQLVTDIREAFGAPVLSGYGLTECPILTMASIDDSDAELAI
                     SEGKPMPGVEIKLVKTDGTIAGPGEDGEIRAKAPQLMKGYLDESLNVDGFDEEGYFRT
                     GDLGRFDDHGNVIITGRLKDIIIRKGENISAKELEDMLFTHPKVADVAVIGLPDAASG
                     ERACAVIQTADGQSDITFDELVEFMLAEGLMKQKLPEQLEIVDVIPRNASGKILKQNL
                     QAQYSKA"
     gene            complement(987..1169)
                     /locus_tag="PLP95_05580"
     CDS             complement(987..1169)
                     /locus_tag="PLP95_05580"
                     /inference="COORDINATES: protein motif:HMM:NF016828.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp family type IVb pilin"
                     /protein_id="HPB45308.1"
                     /translation="MLTSFEFISAYLVAKTKTERGASLVEYALLVALIAVVCIAAVTT
                     LGRKAANNFSSVAGAI"
     gene            1458..2372
                     /locus_tag="PLP95_05585"
     CDS             1458..2372
                     /locus_tag="PLP95_05585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012227274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HPB45309.1"
                     /translation="MGLLEGKVAIVTGAGHGIGRGHALELAKHGAKVVVNDLGVSVTG
                     DGGGKDADLTVDIIKARGGEAVGNYENVADFEGAGRMIQQAIDTYGRLDILVNNAGIV
                     RDSTIVKMSEADFDAVFNVHVKGTWGPAKHAAEYWTAQFKETGKPTGGRIINTTSGAG
                     LTGNFGQSNYASAKAAIASMTQTLSLELFKAGVTVNCVGPAAATRITGTMPGAPKVIE
                     PDEVPEDEWNRMDPAVSSPLVAWLASDEADHVTGQVIRAVAENIILMKGWSDGPTINN
                     GSKRWDATKLGAQLATDVFFTRAPGLRY"
     gene            2439..3671
                     /locus_tag="PLP95_05590"
     CDS             2439..3671
                     /locus_tag="PLP95_05590"
                     /inference="COORDINATES: protein motif:HMM:NF012431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB45310.1"
                     /translation="MQRHYGAIGVGDLELVEVTHADLALLCADADGRISTGWGAALLD
                     QQLLHLSSQPGGTARPRVVTGVEELVHLLVEAELDRTPRTLAFALDFDLDAPAPTDAD
                     FRGRPDGGVASTLSPSLEGLDIVVVVGPGVSREGRETISEFAARGGFGVFNTFGAKGV
                     FRWDSPFHFGTIGLQARDVELAGLRDVDLIIASGLDPDEFDATQLVGRAPVVDIAAWQ
                     LPLALADWPRKHRQVSQRPPLYGLLASVVQPLYEREDAPISPARAALHLSGAAPDGGV
                     VVADAGMAGFWIARTFPTGIENSVVVPALDQPGFAAAAAFVAGLAQRPCVAVIDGPPD
                     DSTLMVLEAAARHQVPVQVQAWVDGDTSVDEHLSLTQRAFGSDEPHLDVVGVDGSATE
                     LLLDVAGEITAWGAGSGS"
     gene            3824..4372
                     /locus_tag="PLP95_05595"
     CDS             3824..4372
                     /locus_tag="PLP95_05595"
                     /inference="COORDINATES: protein motif:HMM:NF036523.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HPB45311.1"
                     /translation="MLNVASRLAREGGYDAVQMRAVANEADVALGTLYRYFPSKEHLL
                     VSAMLRQIESLHERLAVKPPQGLAAMERLVDVLRRANGALQRQQQFTVAVVRALVSGD
                     EMVAPVVRDVRDLMGGIILSALETPDSSEPSVPTQRALLVTEILQEVWLSSLVAWISG
                     VEPASSVDRKLEAAVHLLFGEA"
BASE COUNT          815 a         1412 c         1516 g          772 t
ORIGIN      
        1 acagcctcat catgatggag acgttccacc cgaccgagac cgccgaggtg ctcagccgcg
       61 agaacgtgac cctcgcgggc tccggaaccc cgttccacat ggcctacctc gccgcccaga
      121 aggcatcgtc gacaccgatc tttccgaacg ttcgggcctt cccaggcggt ggcgccccga
      181 agccgccgca actcgtcacc gatattcgtg aagccttcgg cgccccggtg ctgtcgggat
      241 acggcctcac cgaatgcccg atcctgacga tggcgtcgat cgacgacagc gacgccgaac
      301 tcgcgatctc ggagggcaag ccgatgcccg gagttgaaat caagctcgtg aaaaccgacg
      361 ggaccatcgc cggacccggc gaggacggcg agatccgcgc aaaggctcct cagctcatga
      421 aggggtacct cgacgagtcg ttgaacgtcg atgggtttga cgaggagggc tacttccgca
      481 ccggcgacct cggccgtttc gacgatcacg gcaacgtcat cattacgggc cggctcaagg
      541 acatcatcat ccgcaagggc gagaacatct cggccaaaga actcgaggac atgctgttca
      601 cgcatcccaa ggttgcggac gtcgcggtga tcgggcttcc cgatgcggca tcgggggagc
      661 gggcctgcgc ggtgattcag accgccgatg gccaaagcga catcaccttc gacgaactcg
      721 ttgaattcat gctggcggag ggattgatga agcagaagct gcccgagcaa ctcgagatcg
      781 tcgacgtgat cccccgcaat gcgtcgggca agatcctcaa acagaacctc caggcgcagt
      841 acagcaaggc ctaaagggcg cgaccctgcc gttggcgtca cagcaacgaa cgccgcggcg
      901 aacagggcga aaacggcaac gagggccggt ccaggtggac cgaccctcgt cttgctcgaa
      961 cggcgttgcg ccgtccgtcg gctcgctcag atcgcgcctg cgacggagct gaagttgttg
     1021 gcggccttac ggccgagggt cgtcacggca gcgatgcaga ccacggcgat gagcgccacg
     1081 aggagtgcgt actccacgag ggaggcgccc cgctcggtct tggtcttcgc aacgaggtag
     1141 gcggagatga attcgaagct ggtcaacatg tcggatattc cttgcttggg gttgtggtgc
     1201 tttcctgggt cgccatgtcg atcgtgcccg tagggatcat cgttctcagg ctcgcaagtt
     1261 gtatcggggt ggcagtggac gttcttgagg ttcttgaacg ggttactcgg gtgatttggt
     1321 ccgttggccc acaaaccgac gtccgggctg gtccgagggg cctaccgcgc tcgggtcgct
     1381 cacccacgcc tgacacgcgt agaatctgac ggctcgtcag attcagccga atcactcgtt
     1441 acaacccgga gggaaccatg ggcttgctcg aaggcaaggt cgcaatcgtc accggtgccg
     1501 gtcacggcat cggccgtggg catgcgctgg agcttgccaa gcacggggcc aaggtcgtcg
     1561 tcaatgacct gggggtctcg gtcaccggcg acggcggcgg taaggacgcc gacctgacgg
     1621 tcgacatcat caaagctcgc ggaggcgagg ccgtcggcaa ctacgaaaac gtggccgact
     1681 tcgaaggtgc cggacgcatg atccaacagg cgatcgacac gtacggtcgc ctcgacatcc
     1741 tggtcaacaa cgccggcatc gtgcgcgact ccaccatcgt caagatgagc gaggccgatt
     1801 tcgacgcggt gttcaacgtg cacgtcaagg gaacctgggg tcccgccaag cacgccgccg
     1861 aatactggac tgcgcaattc aaagagacgg gcaaaccgac cggtggccgc atcatcaaca
     1921 cgacctcggg cgctgggctc accggcaatt tcggccagag caactacgcc tctgccaaag
     1981 ccgcgatcgc ctcgatgacc cagaccctca gcctcgagct cttcaaggcc ggcgtgacgg
     2041 tcaactgtgt tggcccagcg gcagcgaccc gcatcacggg caccatgccc ggcgccccga
     2101 aggtcatcga acccgacgag gtgcccgagg acgagtggaa ccggatggac ccggccgtaa
     2161 gttctccgct ggtggcctgg ctggcctccg acgaggcgga tcacgtcacc ggtcaggtca
     2221 tccgagcggt cgccgagaac atcatcttga tgaagggctg gagcgacggc ccgaccatca
     2281 acaacggctc gaagcgttgg gacgccacca agctgggtgc ccagctggcc accgacgtgt
     2341 tcttcacccg agcccccgga ctgcggtact aggggcgagc cctgagttcc gcgccgacca
     2401 acgcagcggt cgtcgccgac gcgctgcgag cacttggggt gcagcgtcac tacggcgcga
     2461 tcggcgtggg tgacctcgaa ctcgtcgagg tcacccacgc cgatctcgcg ctcctgtgcg
     2521 ctgatgccga cggcaggatc agcaccggtt ggggtgcggc gctgctggat cagcagctct
     2581 tgcatctgtc ctcgcagccc ggaggaaccg ctcgcccgcg ggtggtcacc ggtgtcgagg
     2641 aactcgtaca cctgctcgtc gaagccgaac tcgaccgaac cccgcgcacg ttggccttcg
     2701 cgctcgattt cgatctcgac gccccggccc cgaccgacgc ggactttcga ggccgccccg
     2761 acggcggtgt cgcgtccacc ctgtcgccgt ccctcgaagg cctcgacatc gtggtggtcg
     2821 tcgggccggg cgtcagccga gaggggcgtg agacgatcag tgagttcgcg gcccgcggtg
     2881 gcttcggtgt attcaacacc tttggcgcca agggagtgtt tcgctgggac tcaccgtttc
     2941 acttcggcac gatcgggttg caggcccgcg atgtcgaact cgccggactc cgagacgtcg
     3001 acctgatcat cgcgagcgga ctcgacccgg acgagttcga cgcgacacaa ctcgtgggtc
     3061 gagcgccggt cgtcgacatc gcggcgtggc aacttccgct ggcgctcgcc gactggccac
     3121 gcaaacaccg gcaggtttca caacggcctc cgctgtacgg actgctcgcg tcggtcgtgc
     3181 agccgctcta tgaaagagag gacgctccga tctcgccggc gcgagcggcg cttcacctgt
     3241 caggggccgc tccggacggt ggggtcgtgg tcgcggacgc agggatggcg gggttctgga
     3301 tcgcacgaac cttcccgacc ggcatcgaga actcggtggt tgtcccagcg ctcgaccaac
     3361 cggggttcgc cgccgcagca gcattcgtcg cggggctggc tcaacgaccg tgcgtcgcgg
     3421 tcatcgatgg cccaccagac gattcgaccc tgatggtgct cgaggcggcg gcgcgacacc
     3481 aagtgccggt tcaggtgcag gcctgggtcg acggtgacac ctcggtcgac gagcacctgt
     3541 cgctgacgca gcgggccttc ggttcggacg agccgcacct cgatgtggtt ggcgtcgatg
     3601 gatcggcgac cgagttgctg ctcgatgtgg ctggggagat caccgcgtgg ggggcgggat
     3661 cgggaagttg aacgcggatc tccggtcggg aaattgtacg agtttccccg aaatcatccg
     3721 atgaagatgc ggaggtgtta gaacaggttc tcattttggg gacgaacggg ggacgggagg
     3781 tgtcgtgtcg ctgacgaagc cacaacagga caggcgtgag cgaatgttga acgtcgcctc
     3841 gcggttggcg cgcgagggcg gctacgacgc ggtgcagatg cgtgcagtgg ccaacgaagc
     3901 cgacgtcgcg ctcgggacgc tgtatcggta cttcccttct aaggaacatc tgctcgtctc
     3961 agccatgctt cgtcagatcg agtcgctgca cgagcggttg gcggtgaagc cgccgcaagg
     4021 actagcggcg atggaacgcc tcgtcgacgt gttgcgtcgc gcgaacggag cgttgcaacg
     4081 tcagcagcaa ttcaccgtcg ccgtggtgcg ggcgctggtg tcgggcgatg agatggtggc
     4141 tcccgtcgtg cgcgacgtgc gcgacctgat gggcggcatc atcctgtcgg cacttgagac
     4201 ccccgactcc tcggagccgt cggtgcccac gcagcgagcg ctgctcgtca ccgagattct
     4261 ccaagaggtc tggttgtcgt cgctggtggc atggatcagc ggagtcgagc cggcgtcgtc
     4321 ggtggaccgt aagcttgagg cagcagtgca tctgctgttc ggggaggcgt agtaggacca
     4381 tggtcgttgt ggaaagtccc aaatgggagc cgttcgagcg gccggtgccc gaaggcgtgt
     4441 cgggtatcga tctcccgctc gggcgaaggc tgaagttgcc ggggcgggga accaccttct
     4501 atcgagaggt tcccg
//
LOCUS       DAOOMA010000161         4462 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_23518_length_4462_cov_4.471296, whole genome shotgun sequence.
ACCESSION   DAOOMA010000161 DAOOMA010000000
VERSION     DAOOMA010000161.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 4462)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4462)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4462
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23518_length_4462_cov_4.471296"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..274
                     /locus_tag="PLP95_05600"
     CDS             <1..274
                     /locus_tag="PLP95_05600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB45312.1"
                     /translation="QLFDFFQRNAATGLVLRGDIGHTPTVLSNDGTTAMVDDCADDRL
                     GVYRVADDSRVDTDDPARRTYTATLRQVDGVWRVERVDAEGTPCAE"
     gene            292..1089
                     /locus_tag="PLP95_05605"
     CDS             292..1089
                     /locus_tag="PLP95_05605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB45313.1"
                     /translation="MTAAVCCLAVLAPSASADGMGGADVDDSGIDYGIITTGANSGGS
                     TIGGDSGTGPTCTYRLMGGPENFPVYDTDGTLIEVQAGGAWYEKTCDGAFVGAVYLTA
                     TPETVNPATLAAGVLRRMTIPIPDVSLSPAVDQIVNLPSWLWISNWAPLTGSATLGGV
                     TVTVTAQPSTARWVFGDGNTSTCAPGVAWSPDANPAEACTHTWTKSSAAERSDSYWLR
                     LAVNWTATYTVSGGAGGGTLPPLTRTAELPVRVAEVQAINGHVGGSR"
     gene            1086..1760
                     /locus_tag="PLP95_05610"
     CDS             1086..1760
                     /locus_tag="PLP95_05610"
                     /inference="COORDINATES: protein motif:HMM:NF020250.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAF domain-containing protein"
                     /protein_id="HPB45314.1"
                     /translation="MTTTTRAPASNGNTPTRTADGAARQSAVKPPRRPNTARIALGAV
                     VVGLSALGAVTLFSNAADRVAVIAVARDVAPGAAVTEADLRNVSISTGEGLDTIDVGD
                     VDRVIGRTAVHGLTAGSLVTPAQLADGPAYADGSVLVGALLSSGQFPVDLEVGDTVQI
                     IETIAPDATGSGDALARGVGTVAALTEALEGQSDRTVSIAVPGDDAAAISAAGAAVRV
                     SLVVTP"
     gene            1757..2482
                     /locus_tag="PLP95_05615"
     CDS             1757..2482
                     /locus_tag="PLP95_05615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB45315.1"
                     /translation="MTGLVCVAAGPGAPGATTVALAAALTAECGSALLVDADGDGGSL
                     AAHLGAATTPGLVTLAAATRHSYSGSELKRHIQPVTGEVGLLAAPSNPEQVSAALIAL
                     GRPFAESLTAHTAVADVGRWRPDNPAECLVGVADATVLAVRPTLAGVASARWSYRDLS
                     ARCAHVVVATRGERPYGPDEVAAAVGCPPSDVVVLPVDRAGASLITNSVSSRWLRRAA
                     LARTIRAMALELWPVASDDGVLV"
     gene            2479..3798
                     /locus_tag="PLP95_05620"
     CDS             2479..3798
                     /locus_tag="PLP95_05620"
                     /inference="COORDINATES: protein motif:HMM:NF012652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HPB45316.1"
                     /translation="MSVAETVDDDLVRSLRTGVAEELSVTARRISADGRRLEGADEEM
                     LARQLIAQRLEQLASDSIADGSPVLSQSEEATLAEAVFNRLFHLGRLQPLLDDESIQN
                     VVANGYDRVFVEYAGGLKIEGPPVAGSDDELIEQLREIGRRYGLSEREFNPSRPQLNL
                     QLPDGSRLFAVAWVCSRPSVSIRRHRYLKLTLADLIGLGAIDEGLSSFLAAAVRSRQQ
                     IIVAGATGAGKTTMLRALASEIPTSERIVTIETELELGLDRFHDIHPDCVALEAREAN
                     VEGAGEVTAADLVRMSLRMNPDRVIVGEVRGDEIVPMLNAMSQGNDGSMCTVHSDSSA
                     TVFNKMALYALQSAERLPLEATCQLAAAAVDLVVYISKSSEGRWVSSVRQVVGADGST
                     VVTNELFRPGHDGRAVPGVPVPHDLAAVLSSHGWDSMMHERREGWWQ"
     gene            3795..>4462
                     /locus_tag="PLP95_05625"
     CDS             3795..>4462
                     /locus_tag="PLP95_05625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB45317.1"
                     /translation="MTVLVVVTVVAAAAGVWLVATGLRPAAPDDGLVGPNAAPASTPG
                     VDRRLRDRALRAAGCALVLAVVSRWPTAVLAGALLGWFSPELFASRHNRDAALARTEA
                     IAAWTEMLRDTIASAHGLEAAITTTAPIAPAAIRPEIEQLARSLKHMPLTDALRGLAV
                     DLAHPISDLVVASLTVAANGAVRDLADLLGMLADSARDEAAMQMRVEASRARMRTAVR
                     VISTV"
BASE COUNT          681 a         1511 c         1539 g          731 t
ORIGIN      
        1 gcagctgttc gacttcttcc aacgcaacgc tgcgactgga ctcgtgttgc gcggcgacat
       61 cggccacaca ccgacggtcc tgtcgaacga cggaaccacc gcgatggtcg acgactgcgc
      121 cgatgaccgc ctcggcgtct accgcgtcgc tgacgacagt cgcgtcgaca ccgacgaccc
      181 ggccagacgg acctacaccg cgacgctgcg ccaggttgac ggcgtgtggc gcgtggaacg
      241 agtggacgca gaggggacgc catgcgccga gtgactcatg cccttctcac tgtgaccgcc
      301 gccgtgtgct gcctcgctgt gctcgctccg tcggcgtccg ccgatgggat gggcggcgcc
      361 gacgtggacg actccggaat cgactacggc atcatcacca ccggtgccaa cagcggtggc
      421 tcgaccattg gtggcgacag cggtaccggc ccgacgtgca cctatcgact tatgggcggc
      481 ccggagaact ttccggtgta cgacaccgac ggcacgctga tcgaggtcca ggccggtggc
      541 gcctggtacg agaagacctg cgacggcgcg ttcgtcggag cggtctacct caccgccaca
      601 cccgagacgg tgaaccctgc gacgctggcc gcgggtgtgc tgcgcaggat gacgatcccg
      661 atccccgatg tctcactcag ccccgcggtc gaccagatcg tgaacctgcc gtcgtggttg
      721 tggatctcca actgggcgcc gctcaccggc tccgcgacgt tgggcggcgt gacggtcacc
      781 gtcaccgcgc aaccctcaac tgcgcggtgg gtgttcggcg acgggaacac ctcgacgtgt
      841 gcgccgggtg tggcgtggag cccggacgcg aaccccgccg aggcgtgcac ccacacatgg
      901 accaagtcgt cagccgccga gcgatccgac tcctactggc tgcgacttgc cgtcaactgg
      961 accgcgacgt acacggtcag cggcggtgca gggggcggca ccctcccccc gctcacacga
     1021 acggcggagc tgccggtgcg cgtcgccgag gttcaggcca tcaacggtca cgttggagga
     1081 tcacgatgac gaccacgact cgcgccccgg cgagcaacgg aaacacgccg acacgaacag
     1141 ccgacggcgc agcacggcag tcagcggtga agccgccacg gcgacccaac accgcacgca
     1201 tcgcactggg tgctgtcgtg gtcggactga gcgcgctggg tgctgtcacg ttgttctcga
     1261 acgctgctga ccgcgtcgct gtgatcgccg tcgctcgtga cgtggcaccc ggcgcagccg
     1321 tgactgaggc cgacctgcgg aacgtgtcga tctcgaccgg agagggactc gacaccatcg
     1381 atgtgggaga cgtcgatcgg gtcatcggcc gtaccgccgt gcacggccta acggcagggt
     1441 cgctggtgac accggcgcag ctcgccgatg gccctgcgta cgcggacggc tcggtgctcg
     1501 tcggagcgtt gctgtcgtct ggccagtttc ccgtcgatct cgaggtcggc gacaccgtcc
     1561 agatcatcga gacgatcgct cccgatgcaa ccggttccgg tgacgcgctg gcgcgaggcg
     1621 tcgggacggt cgcggcgctg acggaggcgt tggaggggca gagcgatcgc accgtgtcga
     1681 ttgcggtgcc cggggatgac gctgcagcga tctcggctgc tggcgctgcg gtccgggtga
     1741 gcctggtggt gactccgtga ccggcctcgt ctgtgtcgcc gccgggcccg gcgctccggg
     1801 cgcgaccacg gtggcgctcg cggccgcact gaccgcagag tgcggttccg cgctgctcgt
     1861 cgatgcggac ggcgacggcg gttcgctcgc tgcgcatctc ggcgctgcga caacgccggg
     1921 gctggtgacg ttggccgcgg cgactcgcca cagctacagc ggctccgagc tgaaacgtca
     1981 catccagccg gtcaccgggg aggtcgggct acttgcagcg ccgtcgaacc cggaacaggt
     2041 atcagccgcg ctgatcgctc tcggccgacc gttcgccgag tcgctgaccg cgcacacagc
     2101 ggtagccgac gtcgggcgct ggcgccccga caaccctgct gaatgcctcg tcggtgtcgc
     2161 agatgcgacg gtgctcgcgg tgcgcccgac cctggcgggg gtcgcgtcgg cacgctggtc
     2221 ctaccgggac ctgtccgcac gctgcgccca cgttgtcgtc gcgacacgtg gtgaacgccc
     2281 ctacggtccc gacgaggtcg ccgcggcggt cggttgccca cccagtgacg tggtggtgct
     2341 gccggtggat cgggcagggg cgtccctgat caccaactcg gtgagcagtc gttggctccg
     2401 tcgcgccgcg ttggcgcgca cgatccgtgc aatggcgctc gagctgtggc cagttgcatc
     2461 tgacgacgga gtgttggtgt gagtgtcgcc gagactgtcg atgacgacct ggtccgctcg
     2521 ctgcgcaccg gggtggccga ggagctgtcg gtcacagcac ggcgtatatc cgccgacggt
     2581 cgccgactcg agggcgcgga cgaggagatg cttgcccgcc agctcatcgc acagcgcctc
     2641 gaacagctcg cgtcggactc gatcgctgac ggcagtcccg tgctgtcgca gagcgaggaa
     2701 gcgacgctcg cggaggcggt gttcaaccgg ctgttccacc tcgggcggct gcagccgctg
     2761 ttggacgacg agtcgatcca gaacgttgtc gccaacgggt acgaccgtgt gttcgtcgag
     2821 tacgcgggcg gcctgaagat cgaagggcca ccggtcgcgg ggtccgacga cgagctgatc
     2881 gagcaactcc gggagatcgg ccgccgctac ggcctgtcag agcgcgagtt caacccctct
     2941 cggccgcagc tcaacctgca gctgccggac ggatcacgac tcttcgcggt cgcgtgggtg
     3001 tgctcgcggc catcggtgtc gatccgccgt caccgatatc tgaagctcac cttggcggac
     3061 ctgatcggcc tcggcgcgat cgacgagggc ctctcctcgt tcctggctgc ggcggttcga
     3121 tcgcgtcagc agatcatcgt cgcgggagcg acaggagccg ggaagaccac gatgttgcgg
     3181 gcgcttgcat cagagatccc gacgtcggag cggatcgtca cgatcgaaac ggagctcgag
     3241 ctcggactgg accgctttca cgacatccac cctgactgcg tcgcccttga agcgcgcgaa
     3301 gccaacgtcg agggcgcagg cgaggtgacc gctgcggacc tcgttcgcat gtcgttgcgg
     3361 atgaacccgg accgggtgat cgtcggcgag gtccgcggtg acgagatcgt cccgatgctg
     3421 aacgcgatgt cgcagggcaa cgacgggtcg atgtgcactg tgcactcgga ctcctcggcg
     3481 accgtcttca acaagatggc gctctacgcg ctgcagtcgg ccgagaggtt gccgctcgag
     3541 gcaacgtgtc agcttgctgc cgcagcagtc gacctcgtcg tctacatctc aaagtcgagc
     3601 gagggccgtt gggtgtcctc ggtgcgacag gtggttggtg cggacggttc gaccgtcgtc
     3661 accaacgaat tgttccgacc cgggcacgac ggccgtgcgg tgccgggcgt tcctgtgcca
     3721 catgacctgg cagccgtgct ctcctcgcat ggctgggact cgatgatgca cgagcggcgt
     3781 gaggggtggt ggcagtgact gtcctcgtcg tcgtgaccgt cgtcgcggcg gcagctgggg
     3841 tgtggctggt cgctaccggc ctccggccag ccgctccgga tgacggactc gtcggaccga
     3901 acgctgcgcc ggcgagcaca ccaggtgtcg atcgacggtt gcgggaccga gcgctgcgcg
     3961 ccgccggatg tgcgctcgtg ttggcggtcg tgtcgcgctg gccaaccgcc gtcctcgctg
     4021 gcgcgctgct cggttggttc tcaccagagc tcttcgcgtc ccgccacaac cgcgacgccg
     4081 cactcgctcg caccgaagcc attgcagcgt ggaccgagat gctgcgcgac accatcgcgt
     4141 ccgcccacgg tctcgaagcc gcaatcacca ccactgctcc gatcgcgcct gcagcgatcc
     4201 gacccgagat cgaacaactc gcccggtcgc tcaagcacat gccgcttacc gacgcactgc
     4261 gaggcctcgc tgtcgacctc gcccacccga tctctgacct cgtcgtcgcg tcgctcacag
     4321 tcgcagcgaa tggggcggtc cgagatctcg ctgatctgct cgggatgctc gccgactctg
     4381 cacgggacga agccgccatg cagatgcgag tcgaagcgtc gcgggcacgc atgcgcacgg
     4441 cggtgcgagt catctccacc gt
//
LOCUS       DAOOMA010000162         4455 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_23564_length_4455_cov_4.513409, whole genome shotgun sequence.
ACCESSION   DAOOMA010000162 DAOOMA010000000
VERSION     DAOOMA010000162.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 4455)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4455)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4455
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23564_length_4455_cov_4.513409"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(163..696)
                     /locus_tag="PLP95_05630"
     CDS             complement(163..696)
                     /locus_tag="PLP95_05630"
                     /inference="COORDINATES: protein motif:HMM:NF024812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase family protein"
                     /protein_id="HPB45318.1"
                     /translation="MEIRAATVDDAEAIREIYNQEVLTSIVTFDLVPRTASEQQAWIT
                     DRSGAHAAIVATDGDDIIGFGSLSPYRSRSGYSTSVEDSVYVHRDHQGRGVGRSLLTA
                     LLEQAERHGFHAVFARIVDGHEASIELHRRLGFEVIGVEREVGRKFGRFLDVVVMETI
                     LGGRVTPTEARVPGPIA"
     gene            complement(736..2115)
                     /locus_tag="PLP95_05635"
     CDS             complement(736..2115)
                     /locus_tag="PLP95_05635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB45319.1"
                     /translation="MRIERLVTESESHTVALDFHPRLTVIGGLDAHDRSRFAEEIVGA
                     LEGYRSGVHLELLSDRGVRSAVFRPARGRHRVVEIDSRSDVTEAFADEAERIDLLARC
                     GLNRTDAAAAMCRSSADLLAVTDHDRLVQGLANVNQSELWVAGEALSASQRRLEAIAN
                     ASAEGSSDNEAAEVIEDQHEELEALRSKATGFFKGTAAVTGVALIGSLVALFIDQLIV
                     IPPLFVMAVAMFARSALLRRAVQSRAREESDALAEFGAQSYLGFHIQRVNGLFDSEQT
                     RRSLMSAAEAHRDAFARWTAIAGDTELAWALRHRSEIDAAARVRRDVFGPDVPTDSPD
                     SMKVIRLAHSTVRHLSELRRVGASGESLPTLFDEVFDGIEAEVLAPLLELLVRSSEQQ
                     QIILLTNSAAVMDWARVEALTGALSVLEPNGAAPGTVDPSGGTSAGTTSGATSGATNE
                     PGASPGVLS"
     gene            2288..2360
                     /locus_tag="PLP95_05640"
     tRNA            2288..2360
                     /locus_tag="PLP95_05640"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2321..2323,aa:Thr,seq:ggt)
     gene            2423..2496
                     /locus_tag="PLP95_05645"
     tRNA            2423..2496
                     /locus_tag="PLP95_05645"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2457..2459,aa:Met,seq:cat)
     gene            2533..3720
                     /gene="tuf"
                     /locus_tag="PLP95_05650"
     CDS             2533..3720
                     /gene="tuf"
                     /locus_tag="PLP95_05650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001040566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HPB45320.1"
                     /translation="MAKEKFERNKPHVNVGTMGHIDHGKTTLTAAISKTLSDRVAGNT
                     ATDFENIDKAPEERERGITINVAHVEYETDARHYAHVDMPGHADYIKNMITGAAQVDG
                     AILVVSAADGPMPQTREHVLLARQVGVPAIVVALNKVDMVDDEELLELVEMEVRELLD
                     SYDFPGDEIPVMPVSALKALEGDSAAQDQVMALMEQVDTYIPEPVRDLDKPFLMPIED
                     VFSITGRGTVVTGKIEQGILHTGDEIEIIGLKDTQKTTCTGVEMFRKLLDEGQAGDNI
                     GALLRGTKKEEVQRGQVLAKPGSITPHTEFEANVYVLGKDEGGRHKPFFSNYRPQFYF
                     RTTDVTGNITLPAGTEMCMPGDNTEMHVELIAPIAMDEGLRFAIREGGRTVGAGRVTK
                     ILK"
     gene            4029..4190
                     /gene="rpmG"
                     /locus_tag="PLP95_05655"
     CDS             4029..4190
                     /gene="rpmG"
                     /locus_tag="PLP95_05655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HPB45321.1"
                     /translation="MANDKRVHVTLECTTCKRRNYITTKNKNNQRERIELKKYCRWDR
                     AHTVHKETR"
     gene            4289..4361
                     /locus_tag="PLP95_05660"
     tRNA            4289..4361
                     /locus_tag="PLP95_05660"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4322..4324,aa:Trp,seq:cca)
BASE COUNT          807 a         1338 c         1501 g          809 t
ORIGIN      
        1 gccctcgcca ctgccgtcgc cgctgccgtt ctgtaggtaa tcgcggcggt tatagccacc
       61 gcgattacct acagaacgcg aacgggggcg ggcgcgggcg ggcgcgggcg ggcgcgggcg
      121 ggcgcgggcg ggcgcgggcg ggcgaacggg ggcgggcgca ggtcaggcga tcgggcccgg
      181 gacgcgggcc tcggtggggg tgacccggcc gccgaggatc gtctccatca cgacaacatc
      241 gaggaaacgc ccgaacttgc ggccgacctc tcgctcgacg ccgatcacct cgaagccgag
      301 tcgtcggtgg agttcgatcg acgcctcgtg gccgtcgacg atccgtgcga acaccgcgtg
      361 gaacccgtgt cgttcggcct gctccaacag ggcggtcaac agcgaccggc ccacaccgcg
      421 gccctgatgg tcgcgatgca catacacaga gtcctcgacg ctcgtcgagt acccggatcg
      481 gcttcggtag ggcgacagcg acccgaaccc gatgatgtcg tcaccgtcgg tcgcaacgat
      541 cgcggcgtgg gctccggacc ggtcggtgat ccaggcctgc tgttcgctcg cggtccgtgg
      601 gacgaggtcg aaggtgacga tcgaggtcaa cacctcttgg ttgtagatct cccggatcgc
      661 ctcagcatcg tcgacggtcg ccgctcggat ctccacggcg ccaagcgtac ctacccgcca
      721 accctgcgga gttcgctacg acaacacgcc agggctcgcg cccggttcgt tcgtcgcccc
      781 gctcgtggcc ccgctcgtcg tcccggcgct ggttccgccg ctgggatcga cggtgcccgg
      841 cgccgcgccg ttcggttcga gcacgctgag cgccccggtc aatgcctcga cccgcgccca
      901 gtccatcacc gcagcgctgt tggtcaataa gatgatctgt tgttgctccg agctgcgcac
      961 caacagttcg agcagcggcg ccaacacctc ggcttcgatg ccgtcgaaca cctcgtcgaa
     1021 cagggtcggc aggctttccc cgctcgcccc cacccggcgc aactcgctca gatggcgcac
     1081 cgtcgaatgg gcgaggcgga tcaccttcat cgagtcgggc gagtcggtgg ggacatcggg
     1141 gccgaacacg tcgcgccgaa cccgggccgc cgcgtcgatc tcgctgcggt gccgcaacgc
     1201 ccacgccagt tcggtgtcgc cggcgatggc ggtccaccgg gcgaacgcgt cgcggtgggc
     1261 ctcggccgcg ctcatcagcg aacggcgggt ctgctcggag tcgaacaggc cgttcacccg
     1321 ctggatgtgg aacccgaggt agctctgggc gccgaactcg gcgagcgcat ccgattcctc
     1381 gcgggcccgg ctctgcaccg ctcggcgcaa caacgcagag cgggcgaaca tcgccacggc
     1441 catcacgaac aacggcggaa tcacgatgag ctggtcgatg aacaacgcca ccagcgatcc
     1501 gatcagggcc acgccggtca ccgcagcggt gcccttgaag aaccccgtcg ccttcgaacg
     1561 caacgcctcg agttcctcgt gttggtcctc gatcacctca gcggcctcgt tgtcgctcga
     1621 cccttcggcc gacgcgttcg caatggcctc gaggcggcgc tgcgaggccg acaacgcctc
     1681 gccggcgacc cacaactccg actgattgac gttcgccagc ccctgcacga ggcggtcgtg
     1741 gtcggtgacg gccaacagat cggcgctgct gcggcacatc gctgcggcgg cgtcggtgcg
     1801 gttcaacccg cagcgggcca acaggtcgat gcgctccgct tcgtcggcga acgcctcggt
     1861 gacatcggaa cgcgagtcga tctcgaccac acgatgacgg cccctcgccg ggcgaaacac
     1921 cgcggagcgg accccccggt cggagagcaa ctcgagatgg acgccgctgc gatacccctc
     1981 gagcgcaccg acgatctcct cggcgaagcg gctgcggtcg tgggcatcca acccgccgat
     2041 gacggtcagg cgcggatgaa agtccagggc gaccgtgtgg gattcggatt cggtgaccag
     2101 tcgctcgata cgcacggtgg gtacttcggc gggggcggcg atgggcttga ggacttgttt
     2161 tgatcggacc cgcaaatggg cgcgcggccg gtccggggcg gtaggttcga aggtcgctga
     2221 cgggttttgt gcgcggcgta cgagccgcta tagtgacccc tcggtcgtcg cccgcgcgcg
     2281 gcgtctcgcc cccttagctc agtcggcaga gcgtttccat ggtaaggaaa aggtcgtcag
     2341 ttcgattctg acagggggct ctcagatttc gaaacgtgtg gcgcaaggcg aatcgccgag
     2401 gtccacccgg tcgaaaccat gtggtggcgt agctcagtcg gtgagagcgc tcgactcata
     2461 atcgagaggt cgtcagttcg agtctgaccg tcactacctc ccccagtagc accgaattca
     2521 aggagattgg ccatggccaa ggaaaagttt gagcggaata agccgcatgt gaatgttggc
     2581 acgatgggcc atatcgatca tggcaagacg acgttgactg ctgcgatttc gaagacgttg
     2641 tcggatcgtg ttgctgggaa cacggcgacg gattttgaga acattgataa ggctccggag
     2701 gagcgtgagc gtggtatcac gatcaatgtt gctcacgttg agtatgagac cgatgcacgg
     2761 cattacgcgc acgtggatat gccgggtcac gcggattaca tcaagaacat gatcacgggt
     2821 gctgcgcagg tggatggcgc gatcttggtg gtgtcggctg ctgatggtcc gatgccgcag
     2881 actcgtgagc acgtgttgtt ggctcgtcag gttggtgtgc cggcgattgt tgttgctctg
     2941 aacaaggtcg acatggtcga tgatgaggag ctgttggagc tggtggagat ggaagttcgt
     3001 gagcttcttg attcctatga cttccctggt gatgagattc cggtgatgcc ggtgtcggcg
     3061 ttgaaggccc tggagggtga ttcggcggcg caggatcagg tgatggcgtt gatggagcag
     3121 gtggatacct atattccgga gccggtgcgt gatttggata agccgttcct gatgccgatt
     3181 gaggatgtgt tctcgatcac gggtcgtggc acggtggtga cgggcaagat cgagcagggg
     3241 attttgcata cgggtgatga gatcgagatc attggtctga aggacaccca gaagacgacg
     3301 tgtactggtg tggagatgtt ccgcaagttg cttgatgagg gtcaggctgg ggacaacatt
     3361 ggtgcgttgc ttcgtggtac gaagaaggaa gaggttcagc gcggtcaggt gttggcgaag
     3421 cctggttcga ttactccgca tactgagttc gaggcgaacg tgtatgtgtt gggtaaggat
     3481 gagggtggcc gtcataagcc gttcttttcg aactatcgtc cgcagttcta tttccgtacg
     3541 accgacgtga cgggcaatat cacgttgccg gctggtacgg agatgtgcat gcctggtgat
     3601 aacaccgaga tgcatgtgga gttgattgct ccgatcgcga tggatgaggg gttgcggttc
     3661 gcgattcgtg agggtggtcg tacggtcggc gcgggccgtg tgaccaagat cctcaagtag
     3721 ggctcacccc ctggttcttg agcggaaccc gctgccgacg acgcacatgc gctcgctcgg
     3781 cggcgggttc cgccgttttt cgtcggggtt cgccggaacc tgacgacaac ctgacaacaa
     3841 cctcgatgac aatgtcgtcg agaaccgagt tggtggcgcg aagtgtgatc gcgtagtgtg
     3901 gtcgctcgtg cctcgggccc tcccgagcgc gcagcgtgca cgctgcgcct gcttccccgg
     3961 accgcaacgg acccggcgag ccccgacatc cggcacgaac gagatctgac gactgaggac
     4021 gactccgaat ggcaaacgac aagcgagttc acgtcacgct cgaatgcacg acctgcaagc
     4081 gacgcaacta catcacgacg aagaacaaga acaaccagcg cgagcgcatc gagctcaaga
     4141 agtactgccg ctgggatcgg gcccacacgg tccacaagga gacccgctga tccatcgggt
     4201 cgttggtgcg cacgacggcg tcgatgacgt ctgcggcgca ccgcggttgc ggcgttcgag
     4261 ccagtaggct ccagcgtcgg cgccaccgag ggcagtagct caattggtag agcaacggtc
     4321 tccaaaaccg taggttgggg gttcgagtcc ctcctgccct gcaaccacga tcgcaggtgc
     4381 agcttccgtc ggcagcgcaa cagacgcacc cacaacattg gtagtgacat ccacatggca
     4441 atgaaccgag agcaa
//
LOCUS       DAOOMA010000163         4442 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_23641_length_4442_cov_4.027354, whole genome shotgun sequence.
ACCESSION   DAOOMA010000163 DAOOMA010000000
VERSION     DAOOMA010000163.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 4442)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4442)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4442
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23641_length_4442_cov_4.027354"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(218..1204)
                     /locus_tag="PLP95_05665"
     CDS             complement(218..1204)
                     /locus_tag="PLP95_05665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020378441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine kinase"
                     /protein_id="HPB45322.1"
                     /translation="MSEPTIDVVGIGNALVDVISSESHEFVASQGLVPGSMNLIDEDR
                     REELYSAMGVGTEVSGGSGANTTVGVASLGGTAHYLGRVRADQLGEVFAHDIKAAGVG
                     YSTPLATDGPATGCCLILVTGDGQRTMNTYLGASSLFCESDVDAEVIRSGRILYLEGY
                     LFDPPAAQQAFRYAAAVAHEAGRQVALTLSDGFCVDRHRDAFRALFDSHVDLVFANES
                     ELCSLYETSDFDAALEQIRDDVPLAVITRSEKGSVIVNGVDTVTVAAAPVERVVDTTG
                     AGDLYAAGFLYGLARGYDLERCGTVASIAAAEVISHVGPRPQVPLAGLVAGR"
     gene            1232..2932
                     /locus_tag="PLP95_05670"
     CDS             1232..2932
                     /locus_tag="PLP95_05670"
                     /EC_number="5.4.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF014877.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016868 - intramolecular transferase
                     activity, phosphotransferases [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HPB45323.1"
                     /translation="MDPRPAEQLESLLRAADAWLAIDPDPATRAELHAVVERTIHHGD
                     ASELDERLSGRLGFGTAGIRGTLGAGPNRMNRLVVRQVAAALASTLHRHVPDLAGRFV
                     LVGRDARHQSEAFAHDVVDVFAAAGISSRLIDGYAPTPLVAWGVRHLGAVAGLVITAS
                     HNPPQDNGIKVYWSDGAQIIPPIDGWVGAAIAECATRDPLGTPVSPEFAPATLADAYV
                     DMVGALIDSGAPPAVRRSEMTIAATALHGVGAHLLGRCLATVGFDDVTFVSEQELPDP
                     DFTTVGSPNPEEPAATERLLFLAERIDADVALALDPDADRLAVAIADGDRRFRVLSGD
                     ELGALLAIGLLERRSPKSAAPALLVTSVVSSRLLAAIAADAGAEFRETLTGFKWLCRP
                     GMERPDLDQVLAYEEAMGYAVGGLRDKDGISAAVATCDLFAGWAGDDRCPQQILDDLA
                     LRYGAHVTHNFSIRPTGPEGRSRVEAATRSLIADPPIAFAEVDVASHDRPAPDVLRWF
                     LANGERIVIRPSGTETKLKCYIEVIEQAAGPHDVQAARTRGERRCRELAEDLRTRLDP
                     "
     gene            complement(2997..3842)
                     /gene="rsmI"
                     /locus_tag="PLP95_05675"
     CDS             complement(2997..3842)
                     /gene="rsmI"
                     /locus_tag="PLP95_05675"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: protein motif:HMM:TIGR00096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HPB45324.1"
                     /translation="MTRPTPTPISLVATPIGNLGDISSRAVKALTDATLIACEDTRRT
                     GQLLRHLGIGHRPFVVLNDHTEREVVDRLVAAATGGERVALVSDAGMPAISDPGYLLV
                     RAAIAAGVDVEVIPGPSASLAALVVSGLATDRFVFEGFLPRKGAERSRRLADVASQPR
                     TVVLYEAPHRVRRTVADLVDVCDPERRVALARELTKLHEEVWRGTLAQAVVHLDEREP
                     RGEYVVVLEGAAAEMATADDVAAELSALLESGMSTKGAAAEVAARHGVPKRDLYGLAL
                     ELQQR"
     gene            complement(3839..4165)
                     /locus_tag="PLP95_05680"
     CDS             complement(3839..4165)
                     /locus_tag="PLP95_05680"
                     /inference="COORDINATES: protein motif:HMM:NF014678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF192 domain-containing protein"
                     /protein_id="HPB45325.1"
                     /translation="MAWLVRDGDVLASLEIADSRRTRMRGLIGLDGFDGALYLPKVTS
                     VHTFGMRFPIDVAFVDEDMAVIRVATLKRWRVTPFVRGAAGVIEAEAGLFQKWGICCG
                     DELGIR"
     gene            complement(4246..>4442)
                     /locus_tag="PLP95_05685"
     CDS             complement(4246..>4442)
                     /locus_tag="PLP95_05685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002183294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HPB45326.1"
                     /translation="MRQLASEGMTMLVVTHEMGFAREVADHVVFMDQGVICEQGPPTE
                     FFANPTRDRTRQFLERVLDH"
BASE COUNT          763 a         1526 c         1418 g          735 t
ORIGIN      
        1 ccaaccaaag ccaggaccca acgaaacatc acacacagcg agcggttccg aacgcaaccg
       61 tgtcaaccct cccggtggga ccgagtccct ctggtgcggt tggcctccga gtcgttcggg
      121 ctgtgggctc gagcgctcgg cggctgcggg tgaacaccgt acaccagatg ttcaccggat
      181 gcacaattcg agacgaagta ttttcctgtg accgtcgtca ccgacccgcg accaggccgg
      241 caagcggcac ctgcgggcgg gggccgacgt ggctgatgac ctcggcggca gcgatggatg
      301 cgaccgttcc gcaccgctcg aggtcgtatc ccctagccag cccatacagg aatccggccg
      361 cgtagaggtc gccggctccc gtcgtgtcga cgacccgctc gacgggcgca gcggcaaccg
      421 tgaccgtatc gaccccgttg acgatgacgg aacctttttc gctgcgggtg atcaccgcca
      481 gcggaacgtc atcgcggatc tgctcaagtg cggcgtcgaa gtcggaggtc tcgtacaacg
      541 agcacaattc cgactcgttg gcaaagacca ggtcgacgtg gctgtcgaac aaggcccgga
      601 acgcgtcgcg atgccgatcg acacagaacc cgtccgagag cgtcaatgcg acctgtcgtc
      661 ctgcctcgtg ggcgaccgcg gctgcgtacc tgaatgcctg ctgcgccgcg ggcgggtcga
      721 agagataccc ctcgaggtag aggatccgcc cggacctgat cacctcggcg tccacgtcgg
      781 attcgcaaaa caggctcgat gccccgaggt aggtgttcat cgtgcgttga ccatcgccgg
      841 taacgaggat caaacagcac ccggtcgcag gtccgtcggt cgccaacggt gtcgaatagc
      901 cgacgccggc ggccttgatg tcgtgggcga acacctcgcc gagttgatcg gcgcgcaccc
      961 gtccgaggta gtgggcggta ccgcccagcg aggcgactcc caccgtcgtg ttggcacccg
     1021 agccgccgct cacctcggta ccgaccccca tcgctgagta gagctcctcg cggcggtcct
     1081 cgtcgatcag attcatcgac cccgggacca ggccttggga agcgacgaat tcgtggcttt
     1141 cggaggagat gacatcgacg agggcgttgc cgatcccgac aacatcgatc gtgggttcac
     1201 tcatggcgag aagcgtagta ggaagacctc catggacccg cggccggccg agcaactcga
     1261 atcgttgttg cgagcggcgg acgcctggct cgcaatcgat cccgacccgg cgacccgcgc
     1321 cgagcttcac gcagtcgtgg agcgcacgat ccaccatggc gatgcctctg aactcgacga
     1381 gcgcctctct ggtcgactcg ggttcggcac cgcaggtatc cggggaaccc tcggcgccgg
     1441 ccccaaccgg atgaatcgcc tcgtcgtacg acaggtcgcg gctgccctcg cgtcgacgct
     1501 gcatcgccac gttcccgacc ttgccggccg gttcgtcctg gtcggccgcg atgcccgtca
     1561 ccagtcggag gccttcgctc atgacgtcgt cgacgtcttc gcagctgcgg gcatctcgag
     1621 tcggctgatc gacggctacg ccccgacgcc gttggtggcg tggggtgtac ggcacctcgg
     1681 tgcagtcgcc ggcctcgtga tcaccgcatc gcacaacccg ccacaggaca acggcatcaa
     1741 ggtgtactgg tccgacggcg cgcagatcat cccgccgatc gacgggtggg tcggtgcggc
     1801 aatcgccgag tgcgcgacac gggatccact cgggactccc gtgtcgcccg agttcgcgcc
     1861 cgcgacactc gccgatgcgt acgtcgacat ggtcggtgcg ctcatcgatt caggcgcacc
     1921 gccagcagtt cgacgctctg agatgacgat cgctgcgacg gcacttcacg gcgtcggggc
     1981 tcacctgctc ggccgctgtc ttgcaacagt cgggttcgac gacgtgacct tcgtctccga
     2041 acaggaactg ccagacccgg acttcacgac cgtcgggtcc ccgaaccccg aggagcccgc
     2101 ggccaccgag cgactcctct tcctcgccga acgcatcgat gcggacgtgg ccctcgcgct
     2161 cgaccccgac gccgaccgtc tcgctgtggc catcgctgat ggcgatcgcc gattccgggt
     2221 gttgagcgga gacgagttgg gtgcgctgtt ggccatcgga ctgctcgagc gtcgctcccc
     2281 caaatctgcg gcgccagcac ttctggtgac ctccgtggtg tcgtcgcggt tgctcgcagc
     2341 gatcgcggct gatgcgggtg cggagtttcg cgaaacactc accgggttca aatggctgtg
     2401 tcggccggga atggagcgcc ccgatctcga tcaggtgctc gcgtacgagg aggcgatggg
     2461 ctatgcggtc ggcggcctcc gcgacaaaga cggcatctcc gcggcggtgg cgacctgcga
     2521 tttgttcgcc ggctgggctg gcgacgatcg gtgcccgcag cagatcctcg atgatctcgc
     2581 gctccgctac ggggcgcacg tgacccacaa cttctcgatc cggccgaccg gacccgaggg
     2641 ccgctctcgt gtcgaggcgg cgacccggtc gctcatcgcc gatccgccga tcgccttcgc
     2701 cgaggtggac gtcgcttccc acgatcgccc cgcccccgat gtgttgcggt ggtttttggc
     2761 caacggcgag cgaatcgtga ttcggccgtc gggcaccgag acgaagctca agtgctacat
     2821 cgaggtgatc gagcaggccg ccgggccaca cgatgtgcaa gccgcccgca ctcgaggtga
     2881 acgccgctgc cgggaactcg ccgaagatct tcgaacccga ctcgatcctt agcgctgggc
     2941 gacagcccga cgccaagggg atgaaggtca acgtttcggc caacgcctca cgctggtcaa
     3001 cgttgctgca gttcgagtgc gagaccgtac agatcccgtt tgggaactcc gtgacgggcc
     3061 gcaacctcgg ctgcggcgcc cttcgtgctc atccccgatt ccagcagtgc cgaaagctct
     3121 gcggccacat cgtcggccgt cgccatctcc gccgcggctc cttccaggac gaccacatac
     3181 tccccccggg gttcgcgttc gtcgaggtgc actaccgcct gagcgagggt gccgcgccac
     3241 acctcctcgt ggagcttggt gagctcccga gcgagcgcaa cgcgacgctc tggatcgcag
     3301 acgtcgacaa ggtcagccac ggttctgcgc acccggtggg gagcctcgta gagcacgacg
     3361 gtacgcggtt gcgatgcgac gtcggcgagg cggcgcgaac gctcagcacc tttacgcgga
     3421 aggaaccctt cgaacacgaa ccgatcggtc gccagtcctg aaacgaccag agcagccaac
     3481 gatgctgacg ggcccggtat gacttcgaca tcgaccccgg cggcgatcgc ggctcgcacc
     3541 agcaggtagc cgggatccga aatcgccggc attccggcat cggagaccaa tgcgacccgc
     3601 tcgccaccgg tcgccgccgc aaccaaacgg tcgaccacct cgcgttcggt gtgatcgttg
     3661 agcacgacga aggggcgatg gccgatgccg agatgccgga gcagttggcc ggtgcgcctc
     3721 gtgtcctcgc acgcgatcag tgtcgcgtcg gtcagggcct tcaccgctcg tgagctgatg
     3781 tcgccgaggt ttccgatggg agtggcgacg agtgagatcg gggtcggggt cgggcgggtc
     3841 atcgaatacc caactcgtcg ccgcagcaga ttccccactt ctggaacagg ccggcctcgg
     3901 cctcgatcac cccggcggcc ccccgcacga acggggtgac ccgccaccgc ttcagagttg
     3961 cgacccgaat cacggccatg tcctcgtcga cgaaggccac atcgatcgga aaccgcatcc
     4021 cgaacgtatg aaccgaggtg accttcggca ggtacagggc gccgtcgaat ccgtcgagac
     4081 cgatcagtcc gcgcatacgc gtccgccttg agtcggcgat ctccagactc gccaggacgt
     4141 caccatctcg tacgagccaa gccacaacgc gagcctaccg aagccgccat gcagcatccg
     4201 ggcgccgatc ggccgttgcg ccggagtcgc cgttgcgccg gagtctcagt ggtcgagcac
     4261 ccgttcgagg aactgccgcg tacggtcccg ggtcgggttc gcgaagaact ccgtcggcgg
     4321 cccctgttcg cagataaccc cctggtccat gaacaccacg tggtccgcaa cctctcgggc
     4381 aaatcccatc tcgtgggtga ccaccagcat cgtcatgcct tccgaggcga gttgacgcat
     4441 ca
//
LOCUS       DAOOMA010000164         4440 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_23656_length_4440_cov_3.080274, whole genome shotgun sequence.
ACCESSION   DAOOMA010000164 DAOOMA010000000
VERSION     DAOOMA010000164.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 4440)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4440)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4440
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23656_length_4440_cov_3.080274"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            83..679
                     /locus_tag="PLP95_05690"
     CDS             83..679
                     /locus_tag="PLP95_05690"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HPB45327.1"
                     /translation="MTAPDVSRRPRSTVRLVLISTMVSMSVALLVTWVFVVRPAARDS
                     ATISPADLVTKLETGVAKVGEPAPVVELTTFDGTTVSSSTFIGKPTVINFWASTCVPC
                     VTEMPMIESVYNDLGDEVNFVGVDVFEAASLGAEMIERTGVTYLQTRDETHEVLGKFA
                     GTAMPHTVVLDAEGRVTAMKSAALRDEAELRAMIDDAR"
     gene            693..1622
                     /locus_tag="PLP95_05695"
     CDS             693..1622
                     /locus_tag="PLP95_05695"
                     /inference="COORDINATES: protein motif:HMM:NF014713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcdA"
                     /protein_id="HPB45328.1"
                     /translation="MNGAALTFAFSAGMVAAINPCGFALLPAYLSYYLGLESATGPES
                     AATGTGTDAPSRSGPQPVIRALSVSAAMTAGFVLVFGLIGVVWTSVSSAIGHRLPWVV
                     VGVGVALVVLGVAMLFGFVPVVRIPKMSTSSGSQSALSAFIFGVSYGVASLSCAIGPF
                     IAAVTTTFRQHNLLDGFMTLLMYALGMGVIIGVLTLAVTFAKVGIINRLRSLIPHMTR
                     LSGVLLIISGFVAIYFGWYEARILTGATPDNSLGRLVTSWRDWVAEFIHNTGEVRIGV
                     IVAIVVLAALSISLVLRHATPPETDAGDSSPAS"
     gene            1679..2413
                     /locus_tag="PLP95_05700"
     CDS             1679..2413
                     /locus_tag="PLP95_05700"
                     /inference="COORDINATES: protein motif:HMM:NF037471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB45329.1"
                     /translation="MTLEFRTAMQAAFPGSIPAADYLDTVRSSLGAVGFDPETTLPLV
                     SICRDELTTNFFARVQEQWGPAFTLAGLGGVPALGATGWGAAFAHIPYTSGRGSLLVF
                     GLPHIGIEDDGTVGMTVREGQDSPTSTCGALTAIYDMSQRGTIPNEVDLDDFEATKLA
                     LRLVVDGHKPDSLVDLTVAALDATEVDLWAAIDAQEIWKDHNVAVCCGVQIHGHGGKD
                     WVWPRSISLTDATGERRQIDAFTPTA"
     gene            complement(2410..3090)
                     /locus_tag="PLP95_05705"
     CDS             complement(2410..3090)
                     /locus_tag="PLP95_05705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020378922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chlorite dismutase family protein"
                     /protein_id="HPB45330.1"
                     /translation="MEPSTPSVGLCVSHLFWMSSPLADADAALEAIATLEAGDDQVVT
                     VSMLGHKSDLCTLVLSRDLWRIRAFQTAMAAAGFDLVDSYQSVTETSEYATHVPEEAK
                     QARLYPQLPIVGKDVFCFYPMSKRRNVGANWYALDYDERMRLMMEHGKSGRSFAGRVT
                     QLITGSTGLDDYEWGVTLFCVHVDDVKEIVYTMRYDEASAQYGEFGTFYIGTVASPAE
                     VLDSLGVG"
     gene            3237..>4440
                     /locus_tag="PLP95_05710"
     CDS             3237..>4440
                     /locus_tag="PLP95_05710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB45331.1"
                     /translation="MFERDGASQHNPDSMNPDSIRPDEISDPSGGWGPLADAVGSCDA
                     LTAQAADLAADVFASLRMMETVNSSLPTGCRESIAAKLSVAAARLAHAAQRLHVAAVG
                     VAASEQVHHRDGVTTIAYWVALHHGLTRRDAQRLLRAGLTTERYEQVRASFEAGDITL
                     GHVDAISSIIPDRYTGERLDAAIDAVRDIEPLLLGTASRCSLNQFERFCRSIRDRLDQ
                     DGPSDPNAEPSRISLSRTFNGRWHLNGDLTADDGAIVATILHDLINRQARDNTNDTSP
                     NSTPPNDTTPSETTAGPEHATAAEPEAGAGDSGAGEAGAGEAERGPTMPERRASAFRD
                     ALIAAAGITRPGRAGVYVHIDLDKLNRADEGLARLFSERHGEPDVSGPAHTETNLDIT
                     DETLWALLA"
BASE COUNT          774 a         1529 c         1335 g          802 t
ORIGIN      
        1 cggcacccga acgccgggtg gctccgacac gattcacagg tccgattcct cgctacgaga
       61 acaccctggg taccgttgat gcgtgaccgc gcccgacgtt tcacgccgcc cccgctcaac
      121 cgtgcgcctc gttctgatct cgacgatggt gtcgatgagc gtcgcgctct tggtgacgtg
      181 ggttttcgtc gtgcgcccgg cggcccgcga ctcggcgacc atctcaccag ccgatctcgt
      241 cacgaagctc gaaaccggcg ttgccaaggt tggcgaaccg gctccggtgg tggagttgac
      301 caccttcgac ggcacgaccg tttcgagttc aaccttcatc ggaaagccga cggtcatcaa
      361 cttctgggcg tcgacctgtg tcccctgtgt caccgagatg ccgatgatcg aatcggtgta
      421 caacgacctc ggagacgagg tgaatttcgt cggagtcgac gtgttcgaag cggcatcgct
      481 cggcgccgag atgatcgagc gaaccggcgt cacctatctg cagacacgcg acgaaaccca
      541 cgaggtgctc ggcaaattcg ccggcaccgc gatgccccac accgtcgtgt tggatgccga
      601 aggccgggtg accgcgatga aaagcgctgc gcttcgcgac gaggccgagc tgcgagccat
      661 gatcgacgac gcacgatgaa gcgtcggctc acatgaacgg agcggcactc acctttgcct
      721 tctccgccgg gatggtggcc gccatcaacc cgtgtgggtt cgccctgttg cccgcctacc
      781 tctcctatta cctcggcctc gaatccgcaa cgggccccga atccgccgcc accggcaccg
      841 gcaccgatgc gccgtcacgg agcggacctc agcccgtcat tcgagcactc tcggtcagcg
      901 ccgcaatgac ggcagggttc gtcctggtgt tcggactcat cggggtggtg tggacctcgg
      961 tgtcctcagc gatcggacac cggttgccat gggtcgtggt cggggtcggg gtcgcgctcg
     1021 tcgtcctcgg ggtagcgatg ctcttcgggt tcgttccggt cgttcgcatc ccgaaaatgt
     1081 ccacctcgtc ggggtcacaa tcggcgttga gcgccttcat cttcggggtc agctacggcg
     1141 tcgcctcgtt gagttgtgcg atcggcccgt tcatcgctgc ggtcaccacc acgtttcggc
     1201 aacacaacct cctcgacggg ttcatgaccc tgttgatgta cgcactcggc atgggagtca
     1261 tcatcggtgt tctcacgttg gcggtgacct tcgccaaagt cggaatcatc aaccggctgc
     1321 gctcactgat cccccacatg acccgtctga gcggcgtgct gttgatcatc tccgggttcg
     1381 tcgcgatcta cttcggctgg tacgaggctc gaattctcac cggagccacg cccgacaaca
     1441 gcctcgggcg tctggtgacg tcttggcgtg actgggttgc cgagttcatt cacaacaccg
     1501 gcgaagtacg aatcggtgtc atcgtcgcca tcgtcgtcct cgctgctttg agcatttcgc
     1561 tggtgttgcg ccacgcgacc ccacccgaaa ccgacgcggg cgactcgtcc cccgcttcgt
     1621 gacgattgat cggcccgctt ggcgccggcg cgtgccacgg gttcagactg gatatttgat
     1681 gacactcgaa tttcgtaccg ccatgcaagc agcgttcccc ggctcgattc ccgcagccga
     1741 ctatctcgac accgttcggt cgtcgttggg ggcggtcgga ttcgatccgg agaccacgtt
     1801 gccgctcgtc agcatctgcc gcgacgaact caccacgaac tttttcgccc gggtccagga
     1861 gcagtggggt ccggccttca ccctcgccgg cctcggcgga gttccggcac tgggcgcgac
     1921 cgggtggggg gcggcgttcg ctcatattcc gtacacgagc ggacgcggaa gcctgctggt
     1981 gtttggactt ccccacatcg gcatcgaaga tgacggcacc gtcggcatga ccgtgcgcga
     2041 ggggcaggac tcccccacgt ccacctgcgg cgcactcacc gcgatctacg acatgtcaca
     2101 acgaggcacc atcccgaacg aggtcgacct cgacgacttc gaagcgacca agctcgccct
     2161 gcgcctcgtc gtcgatggcc acaagcccga ctcgctcgtc gacctgaccg tcgccgcgct
     2221 cgacgcgacc gaagtcgacc tctgggcagc gatcgacgca caggagatct ggaaggacca
     2281 caacgtcgcg gtctgttgcg gcgtgcagat ccacggccac ggcggcaagg actgggtgtg
     2341 gccgcgaagc atctccctga ccgacgccac cggtgagcgc cgtcagatcg acgccttcac
     2401 cccgaccgcc taaccgaccc caaggctgtc gagcacctct gcgggcgagg caaccgtgcc
     2461 gatgtagaag gtgccgaact cgccgtactg ggccgaggcc tcgtcatacc gcatcgtgta
     2521 gacgatctcc ttgacgtcat ccacatgcac gcaaaacagg gtgacacccc actcgtagtc
     2581 gtcgaggccg gtggagcccg tgatgagttg ggtgacccgt ccggcgaagc ttcgcccact
     2641 cttgccgtgc tccatcatca gccgcatccg ctcgtcgtag tcgagcgcat accagttggc
     2701 tcccacattt cgacgcttcg acatcggata gaagcaaaag acgtccttgc cgacgatcgg
     2761 caactgtgga tacaggcgcg cctgcttggc ttcctcgggg acgtgggtgg cgtactcgga
     2821 ggtttcggtg acggactggt agctgtcgac gagatcgaac ccagccgcag ccatcgccgt
     2881 ttggaacgct cgaatccgcc acaaatcccg ggagagcacg agggtgcaca ggtcggactt
     2941 gtgtccgagc atcgacacgg tgacgacttg gtcgtcgccc gcttcaagcg tggcgatggc
     3001 ctcgagcgcg gcgtcggcgt ccgccagcgg cgacgacatc cagaacagat gagacacaca
     3061 caggccgacc gagggagtgg agggttccat aggccttgag cctacgtgag cgccgccgcc
     3121 ggctcggtgc gcagcgtggg ctggcctggg acggacccca cctttaggtc gaacatttgt
     3181 tttgtttctc ctcgttttcg cttgctgatc gtactgttgt tcgatattct cgtaccatgt
     3241 tcgagcgcga cggggcctcc caacacaacc cagattcgat gaacccagat tcgatccgcc
     3301 ccgacgagat cagcgaccct tcgggcggtt ggggtccgct cgccgacgcc gtcggctcct
     3361 gcgatgcact caccgcgcaa gctgccgacc ttgccgccga cgtgtttgcc tccctgcgga
     3421 tgatggagac cgtgaacagc tccttgccga cgggatgtcg ggagtcgatc gctgccaaac
     3481 tttccgtcgc cgctgcccgg ctcgcccacg ccgcccaacg gctccacgtc gccgcggtcg
     3541 gggtcgccgc atccgagcag gtccatcatc gcgacggggt caccaccatc gcctactggg
     3601 tcgcgttgca ccatggcctg acccgccgcg acgctcaacg actgctccgt gccgggctca
     3661 ccaccgaacg ctacgagcag gtacgcgcct cgttcgaagc cggcgacatc acactcggcc
     3721 acgtcgacgc gatctcatcg atcatccccg acaggtatac gggtgagcgt ctcgacgcag
     3781 ccatcgacgc agttcgcgac atcgaaccgc tgctgctcgg caccgcatct cgatgctcac
     3841 tcaaccagtt cgaacgcttc tgtcgctcca tccgcgaccg actcgaccag gacggcccca
     3901 gcgacccgaa cgcggaaccc tcgcgtatct cgctgtcgcg gacgttcaac ggacgttggc
     3961 acctcaacgg cgacctcacc gccgacgacg gggcgatcgt ggcaacgatc cttcacgacc
     4021 tcatcaaccg gcaggcccgc gacaacacca acgacacgtc gcccaacagc accccgccca
     4081 acgacacaac gcccagcgaa accactgccg ggcctgagca cgcgactgcg gctgagccgg
     4141 aggctggtgc tggcgactct ggagctggcg aggccggtgc tggcgaggcc gaacgcggac
     4201 cgacgatgcc cgaacgcagg gcctcggcgt ttcgcgatgc gttgatcgcc gctgccggaa
     4261 tcacacgacc cgggcgcgcc ggggtctacg tccacatcga cttggacaag ctcaaccgag
     4321 ccgatgaagg tctcgcgcgg ctgttcagcg aacgacacgg cgaacccgat gtttccggtc
     4381 ccgcgcacac cgaaaccaac ctcgacatca ccgacgagac cctgtgggcc ctcctcgcca
//
LOCUS       DAOOMA010000165         4436 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_23682_length_4436_cov_3.942022, whole genome shotgun sequence.
ACCESSION   DAOOMA010000165 DAOOMA010000000
VERSION     DAOOMA010000165.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 4436)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4436)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4436
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23682_length_4436_cov_3.942022"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..764
                     /locus_tag="PLP95_05715"
     CDS             <1..764
                     /locus_tag="PLP95_05715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB45332.1"
                     /translation="TNTTGLAQVGSTVTVTGTGFDPALITPPGTPANAAVAGVYVAIG
                     IGAGPVPTAYTSAKYIRPTGPSPETASSAKLEADGSFSATINTVALFNGQGQTVNCYI
                     DACNVYVWSAHMGTVPSWSFSAPVTFAAPTETQMLVSKSADLANGETVTVSGVGYQAA
                     GPGIYVGQMPWTQSALPADWSTNSAAWGPTKFLPFPSQVNASGAWRTTLATAKNIGEV
                     DCSTADSCSIGSVRAHGTADPTGQNTAFATITFAP"
     gene            complement(891..1604)
                     /locus_tag="PLP95_05720"
     CDS             complement(891..1604)
                     /locus_tag="PLP95_05720"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HPB45333.1"
                     /translation="MARSLGRLLAAALLIISLVGCSDSTPQSGTHPSAGCDGEARVMF
                     AGDSIMVETVARLGFPTGVVWGSVATGSSGFTRPLTGDDSTSRNIGERVLAALDSCNS
                     EVDLVVFNGGINDLMSAQPLAPLVDAVTALDDELAARGIATVWLPITPWVVSTLTPFD
                     HRYERRLEYNDWLRAAKGNDRVLECNDVLASPGAAVEQMNSAYRTMADLVTPDRVHPS
                     SAGYQVYANCLTPKLAALY"
     gene            1750..2577
                     /locus_tag="PLP95_05725"
     CDS             1750..2577
                     /locus_tag="PLP95_05725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MTAP family purine nucleoside phosphorylase"
                     /protein_id="HPB45334.1"
                     /translation="MFPLPPSDDPALAEPVDLAIFGGSGFYSFVEGLTPIPLDTPYGP
                     PSSVPMVGSVDGRRVAFIARHGIHHTIPAHRVNYRANVWAFARLGARAVISPFACGAL
                     RPDLGVGDLVVVDQLVDRTSRREGTFFDGPETVHLTFADPYDPTMGRELADAADGLGL
                     RVHRGGTVVVIDGPRFSTRAESRWHAAQGWDLVNMTQAPEVALVREAAMASLGVGIIT
                     DHDAGLEGEPDVAPVTGEQVLATLRHNAEVLKSLLRTTIGSLTLPDSVGAQDQSWEA"
     gene            complement(2559..4130)
                     /locus_tag="PLP95_05730"
     CDS             complement(2559..4130)
                     /locus_tag="PLP95_05730"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit N"
                     /protein_id="HPB45335.1"
                     /translation="MLTHILQDPGITPISADGVELATPKIEWGYMTPLLVLFVGVVVF
                     LVAMSLIRKMLTPAVAAGLTIVIAAASLVSTIPLWRRVENPAEGPIGAIGGVFGVDRF
                     SLYVTGLICVAVILVALLLADYMRREEIVGPELFLLMILSAAGGVIMAGANDLIVLFI
                     GLEVLSISAYVMCAIHDRRLSSQEAGFKYFVLGGFASAFLLYGVAMVYGATGSTSLLH
                     ISDFLADNLLEHDGLLLAGLALMLVGFGFKIAGAPFHMWAPDVYQGAPSPISGFMSSA
                     VKVAAFAGLLRVFVVGFSTQAEQWRPIVFAIAVLSMLVGAIMAVTQTNVKRMLAYSSI
                     NHAGFILLGVEAATARGTSASLFYLAAYTVMTIGSFGVVTLVGRKGDGRHNLSDYRGL
                     GHSRPGVALVFTVFLLAQAGTPFTAGFVAKVGVISAATSVGHWAIAIVAMVSATISCF
                     LYLRIVASMFFIGGGDHGDEPLELEGPPVRVPVSAALSLAIALVITVGMGVLPGIVSG
                     WSGDAEPALVYASQD"
     gene            complement(4130..>4436)
                     /locus_tag="PLP95_05735"
     CDS             complement(4130..>4436)
                     /locus_tag="PLP95_05735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015439980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB45336.1"
                     /translation="IFASLYLLWAYQRVFHGEPSEDNESFGELKASEAMGLLPLLVLI
                     VVLGVYPKPVLDRIEPSVDALMEHMGTHESSIDVTDVVSADLTGAESAAADGEEGGH"
BASE COUNT          831 a         1636 c         1336 g          633 t
ORIGIN      
        1 tcaccaacac caccggtctg gcccaggtcg gatccaccgt cacggtgacc ggcaccggct
       61 tcgacccggc gttgatcacc ccgcccggga cccccgccaa cgcggctgtc gcaggcgtgt
      121 acgtcgccat cggcatcggc gctggtccgg ttcccaccgc ctacacgagc gcgaagtaca
      181 tccgacccac gggtccgtcg ccggaaaccg cgtcgagcgc caagctggag gccgacggaa
      241 gcttctcggc caccatcaac accgtggcgc tgttcaacgg ccagggccag accgtcaact
      301 gctacatcga cgcctgcaac gtctacgtgt ggtcggcgca catgggcacg gtcccgtcgt
      361 ggtcattctc ggccccggtg accttcgcgg caccgaccga aacccaaatg ctggtctcga
      421 aatcggccga cctcgcaaac ggcgagaccg tgaccgtgag cggcgtcggc taccaggccg
      481 ccggaccggg catctacgtc gggcagatgc cttggaccca atccgcgctc cccgccgact
      541 ggagcaccaa ctccgccgcc tggggtccca ccaagttcct gcccttcccc agccaggtga
      601 acgcgtccgg cgcatggcgc accaccctcg ccaccgccaa gaacatcggc gaggtcgact
      661 gctccaccgc cgactcgtgc agcatcggct cggtgcgcgc ccacggcacc gctgacccga
      721 cgggccagaa caccgcgttc gcgacgatca ccttcgcgcc gtagccgaca gccaccagcc
      781 gacagccgac agccgacagc caccaggcaa ctggcgcccg gctacccggg tacccagcta
      841 cacggtttca gggtctgcgt cgcaacgccg gccctgaaac cgtgcccggc tcagtagagc
      901 gctgcgagtt tcggagtcag gcagttcgca tacacctggt agccggcgga ggagggatgc
      961 acccgatcgg gggtcaccag atccgccatc gtgcgatatg cgctgttcat ctgctcgacg
     1021 gcggcgccgg ggcttgccag cacgtcgttg cactcgagca cacggtcgtt gcccttcgct
     1081 gcacgaagcc agtcgttgta ctcgaggcgg cgctcgtagc ggtgatcgaa cggggtcaac
     1141 gtcgacacca cccagggggt gatcggcagc cacacagtcg caatgccgcg ggcggcaagc
     1201 tcgtcgtcga gtgcggtcac cgcgtcgacg agcggtgcga gcggttgagc cgacatcagg
     1261 tcgttgatgc cgccgttgaa caccacgaga tcgacctcgg agttgcagga atcgagcgcc
     1321 gccagcaccc gttcgccgat attgcggctc gtcgagtcgt cgccggtgag cggacgggtg
     1381 aacccggagc tgccggttgc gacgctgccc cacacgacac cggtggggaa cccgagccgg
     1441 gcgacggttt cgaccatgat cgagtcgccg gcgaacatga cccgggcctc gccatcgcag
     1501 ccagcgctcg ggtgcgtgcc cgactggggc gtgctgtcgc tgcaacccac gagagaaatg
     1561 atcaacaatg cggcggcaag cagccttccc aacgaacgcg ccacgctgta actctcccac
     1621 ctcggggaca cccgttccgg gtctccgtgc cctttgatga tactgagaac gatctcagag
     1681 atccagaggt tgtttcggtg cggatgctcc aatgccgccg atctccgggc cgcgcctacc
     1741 attcgcccca tgttcccact gccgccctcc gacgaccccg ccctcgccga accggtcgac
     1801 ctcgccatct tcggcgggtc cggcttctac tccttcgtcg aagggttgac cccgatcccg
     1861 ctcgacaccc cctacggtcc gccctcctcg gtgccgatgg tcggctcggt cgacgggcga
     1921 cgcgtcgcct tcatcgcccg ccacggaatc catcacacca tccccgccca ccgggtgaac
     1981 taccgagcca acgtctgggc cttcgcccgg ctcggcgccc gcgccgtcat ctcgccgttc
     2041 gcctgcggag ccctgcgacc cgacctcggc gtgggagacc tcgtcgtcgt cgaccaactc
     2101 gtcgaccgga cttcacggcg cgagggcacc ttcttcgacg gacccgaaac cgtgcacctc
     2161 accttcgcgg acccctacga cccgaccatg ggccgcgaac tcgcggacgc cgccgatggg
     2221 ctcggccttc gcgtgcaccg cggcggaacc gtcgtggtga tcgacggtcc tcggttctcc
     2281 acccgcgccg agtcgcgctg gcacgccgct cagggatggg acctcgtcaa catgacccag
     2341 gcccccgagg tggcgctcgt tcgcgaggcc gccatggcct cgctcggcgt cgggatcatc
     2401 accgatcacg acgccggcct tgaaggcgaa cccgacgtcg cgccggtgac cggcgaacag
     2461 gtgctcgcca ccctgcgcca caacgccgag gtgttgaagt cgttgctgcg aaccacgatc
     2521 ggttcgctca cgctccccga ctcggtcggg gcccaggatc agtcctggga ggcgtaaacc
     2581 agcgccggtt ccgcatcgcc cgaccaaccg gacacgatgc cgggcagcac gcccatcccc
     2641 acggtgatca cgagtgcgat ggccagcgac agcgccgcgc tcaccggcac ccgcaccggc
     2701 gggccttcga gttcgagcgg ctcgtcgccg tggtcgccgc cgccgatgaa gaacatcgac
     2761 gccacgatgc gcagatacag gaaacacgaa atggtcgcgc tcaccatcgc cacgatcgcg
     2821 atcgcccagt ggcccacgct cgtcgccgcc gagatcaccc cgaccttcgc cacgaacccg
     2881 gccgtgaacg gcgtgccggc ctgagccaac agaaacaccg tgaacaccag cgccacaccc
     2941 ggacgcgaat ggccgaggcc ccggtagtcc gacaggttgt gacggccgtc gcccttgcgg
     3001 ccgaccaggg tcaccacgcc gaagcttccg atcgtcatca ccgtgtaggc ggccaagtag
     3061 aacagcgagg ccgaggtgcc ccgcgccgtg gcggcctcga cccccaacag gatgaacccg
     3121 gcgtgattga tcgagctgta ggccaacatg cgcttcacgt tggtctgggt gaccgccatg
     3181 atcgccccga cgagcatcga tagcaccgcg atggcaaaca cgatcggacg ccactgctcg
     3241 gcctgggtgg agaacccgac gacgaacacc cgcaacagcc ccgcaaatgc cgcgaccttc
     3301 accgcggaac tcatgaaccc gctgatcggc gacggcgcac cctgatagac gtcgggagcc
     3361 cacatgtgga acggggcacc ggcgatcttg aatccgaacc cgaccaacat gagcgccagg
     3421 ccggccaaca acagcccgtc gtgttccaac aggttgtcgg ccaggaaatc cgagatgtgc
     3481 aacaggctgg tcgaaccggt cgccccgtac accatggcca cgccgtagag caggaacgcc
     3541 gaggcgaagc cgcccaacac gaagtacttg aagccggcct cctggctgct gaggcgacga
     3601 tcgtggatcg cacacatcac ataggccgag atcgacaaca cctcgaggcc gatgaacaac
     3661 acgatcaggt cgttcgcccc cgccatgatc accccgccgg ccgcggacag gatcatcaac
     3721 aggaacagct cggggccgac gatttcttcg cggcgcatgt agtcggccaa caacagggcc
     3781 accaagatga ccgccacgca gatcaaaccg gtgacataca acgagaaccg gtcgacgccg
     3841 aacacgccgc cgatcgcacc gatcgggccc tcagccgggt tctccacccg gcgccacaac
     3901 ggaatcgtcg acaccagcga cgccgcggcg atcacgatcg tcaacccggc agcgaccgcc
     3961 ggggtgagca tcttgcggat gagcgacatg gcgacgagga acaccaccac gccgacgaac
     4021 aacaccagta gcggggtcat gtacccccac tcgatcttcg gagtggccaa ctcgacgccg
     4081 tcggccgaga tcggggtgat ccccggatcc tgcaggatgt gcgtcaacat cagtgtccac
     4141 cttcttcacc gtcggcggcc gcgctctcgg cgccggtaag gtccgcgctc acgacatcgg
     4201 tcacgtcgat cgacgactca tgggtgccca tgtgctccat cagcgcgtcc accgacggct
     4261 cgatgcgatc gagtaccggc ttcggataca cgccgagcac cacgatcagc accagcagcg
     4321 gcaacaaccc catggcctcg gaggccttca gctcgccgaa ggactcgttg tcctccgacg
     4381 gctcgccgtg aaacacccgc tgataggccc acagcaggta cagcgaagcg aagatg
//
LOCUS       DAOOMA010000166         4398 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_23970_length_4398_cov_3.629289, whole genome shotgun sequence.
ACCESSION   DAOOMA010000166 DAOOMA010000000
VERSION     DAOOMA010000166.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 4398)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4398)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4398
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23970_length_4398_cov_3.629289"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..600)
                     /locus_tag="PLP95_05740"
     CDS             complement(<1..600)
                     /locus_tag="PLP95_05740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015799332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIR synthase related protein"
                     /protein_id="HPB45337.1"
                     /translation="MAAPVDVPVHQALGLTDSELDDIIETLGREPNHLELAMYSVMWS
                     EHCSYKSSRVHLKRLPTEAEWVMVGPGENAGVVDVGDGIALAMRIESHNHPSAIEPYQ
                     GAATGVGGILRDIFTMGARPIALMDPLRFGSLDDARSRWIAEGVVSGISGYGNSVGVP
                     TVGGETVFDDTYADNPLVNVLCLGLLPRERLVLGSATGVG"
     gene            899..1279
                     /locus_tag="PLP95_05745"
     CDS             899..1279
                     /locus_tag="PLP95_05745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB45338.1"
                     /translation="MSDAHKEALAAGRHQSRAVKTYLTALQSNKPKRGRKRTTESITK
                     RLGAIADELPTADALQEVLLLQEQANLEKELSTIDTDVDITELEAEFIKVGQAYSKAK
                     GIEYSTWRKVGVSAEVLAKAGIKR"
     gene            complement(1347..2015)
                     /gene="purQ"
                     /locus_tag="PLP95_05750"
     CDS             complement(1347..2015)
                     /gene="purQ"
                     /locus_tag="PLP95_05750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HPB45339.1"
                     /translation="MAVTVGVVQFPGTNCELDVIEGVASLGAQGSILWHGDDSVGSVD
                     AVVIAGGFAHGDYLRPGAIARFSPIMAAVAQFAADGGPVVGICNGFQVLTEAGLLPGA
                     LQKNAGLKFKCAETELRVETTDSVLTGDARAGQVLRMPINHFEGNYTCSAETLAQLRD
                     EDRVVVRYVDNPNGSIDNIAGVCSAGRNVVGLMPHPERATHELLGSTDGRVLLGSLLS
                     AAAQ"
     gene            complement(2017..2271)
                     /gene="purS"
                     /locus_tag="PLP95_05755"
     CDS             complement(2017..2271)
                     /gene="purS"
                     /locus_tag="PLP95_05755"
                     /inference="COORDINATES: protein motif:HMM:NF004630.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HPB45340.1"
                     /translation="MIFDCHVDVQLRPGIADPQGSTIERAMPALGFDGVSGVRVGKSI
                     RFQVEASDEASARSEVEDMCARFLTNPVIEDAVIDLVERS"
     gene            complement(2345..3229)
                     /locus_tag="PLP95_05760"
     CDS             complement(2345..3229)
                     /locus_tag="PLP95_05760"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HPB45341.1"
                     /translation="MIDLGLPHIYSGKVRDIYDAGDGRWVMVASDRISAFDVVMNEPV
                     PNKGRVLTAMSAFWFEQFADLVGSHLISTDLADLPEGARNDEIAGRFMLVRKAEMLPI
                     ECIVRGYITGSAWKEYKNHSTMHGQRLAAGLQESDKLPEPVFTPSTKAEVGDHDENIS
                     FERAVDIVGAELASKARDLSIELYRRGSELAAQRGIIIADTKFEMGIIDGELVLADEV
                     LTPDSSRFWPVDSWIPGTTPPSFDKQPVRDYLETLDWDKTPPPPQLPGEVVAASSQRY
                     AEAYERVSGRRLSEWPGA"
     gene            complement(3263..>4398)
                     /locus_tag="PLP95_05765"
     CDS             complement(3263..>4398)
                     /locus_tag="PLP95_05765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018555222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lyase family protein"
                     /protein_id="HPB45342.1"
                     /translation="QRLLGEFADPIAQLQIRLALELVRDRMVSALAKLGSRVGEFAEQ
                     PLTGRSHNVAAQTTTLGKRFANAGQELLVAFDRIEDLLERYPLRGLKGPVGTQQDQMD
                     LLGGDARAMGELEARVAAHLGFGHVLTNVGQVYPRSLDFDVVAALVQAAAAPSSLATT
                     MRLMAGHDLVTEGFKPGQVGSSAMPHKMNSRSCERICGFHTILNGYLTMVGGLAGDQW
                     NEGDVSCSVVRRVALPDAFFAIDGLFETLLTVLDELGAYPAVIQRELDRYLPFLATTK
                     VLMAAVQHGVGREVAHEVIKEHAVAAALAMREHLDGSNDLVERLETDDRLGLAPGTLA
                     ALFAEPLRFTGNAVAQSAAFVDQVEALARRYPAAAAYAPAPIL"
BASE COUNT          856 a         1427 c         1348 g          767 t
ORIGIN      
        1 ccccacgccg gtggccgatc ccagcaccag acgttcccga ggcagcaacc ccaggcagag
       61 gacgttgacg agcggattgt ccgcataggt gtcgtcgaag accgtctcgc cgccaacggt
      121 gggcacgccg actgagttgc cgtatccgga gatcccggag accacacctt ctgcgatcca
      181 ccggctgcgg gcgtcgtcga gcgacccgaa ccgcaacggg tccatcaacg cgatcggccg
      241 tgctcccatc gtgaagatgt cgcgcagaat cccacctacc ccggtcgctg ctccctgata
      301 cggctcgatc gcggaggggt ggttgtggct ctcgatacgc atcgccagcg cgatcccgtc
      361 gccgacatcg accactccgg cgttctcgcc aggccccacc atcacccact cggcctcggt
      421 ggggaggcgc ttcaggtgga cccgcgacga cttgtaggaa caatgttccg accacatcac
      481 cgagtacatc gccaattcca gatggttggg ttcgcgtccc agcgtctcga tgatgtcgtc
      541 aagctcactg tcggtgagcc ccaacgcttg gtggacggga acatctacgg gtgcggccat
      601 ggcgaaaatc tagcgggcaa ccggcggtgg gtcggccgtc gcggttgcga caccgggtga
      661 tgccgccttc gcgatttcgg taatgcggtg aaatacggtt gatggagatg ctggcgaagt
      721 cagatcaaca aatcaacggg tgtccaccgg caccgaaata ggccgtgtaa catttggacg
      781 ttcgctcgat ctgacgatgg cctggtaagt gtgcggatcg gcccccgttg gcgacgagcg
      841 tcgccccaag taatccatga cggagatgtg aataatgcaa gacggtacga cacgtcctat
      901 gagcgacgcg cacaaggaag cattggccgc cggacgccac cagagccgag cggtcaagac
      961 gtatctcacc gcgcttcagt ccaacaagcc gaagcgtggc cgcaagcgca ccacagaatc
     1021 gatcaccaag cgtctcggcg cgatcgccga cgaacttccg accgcagatg cgctgcaaga
     1081 agttctcttg ttgcaggagc aggccaacct cgaaaaggaa ctctcgacca tcgatacaga
     1141 tgtcgatatc accgaactcg aagccgagtt catcaaggtt ggtcaggcat actccaaggc
     1201 caaaggcatc gagtattcga cctggcgaaa ggtcggcgtg agcgccgagg tcttggccaa
     1261 ggccggcatc aagcgctaaa gccccgcggg cgatccgccg acgaccggtc catcccgttc
     1321 gtcggcggat cgaacaccgc cgggctttac tgtgctgctg cgctcagaag cgatcccagc
     1381 aacacgcgac catcggttga gcccaacaac tcgtgggtgg cccgttcggg gtgcggcatc
     1441 agccccacga cattgcgacc ggcggaacag accccggcga tgttgtcgat cgagccattc
     1501 ggattgtcga cgtaacgcac cacgacgcgg tcctcgtcac gcagttgagc caacgtctcg
     1561 gccgaacagg tgtaattgcc ctcgaaatga ttgatgggca tgcgaaggac ctgaccagct
     1621 cgggcgtctc ccgtcaacac tgaatcggtc gtctcaaccc gcagttccgt ctcggcgcac
     1681 ttaaatttga gaccggcgtt tttctgcaac gccccgggca gcagccctgc ttcggtcaga
     1741 acctggaacc cgttgcagat cccgacgacc gggcctccat ctgccgcgaa ttgggcgacc
     1801 gccgccatga tcggcgaaaa acgggcgatg gccccggggc ggaggtaatc gccatgggcg
     1861 aacccaccag ctatcaccac ggcatccacc gaccccaccg agtcatctcc gtgccacaga
     1921 atggaacctt gggctccgag cgaggcgacg ccttcgatca cgtcgagttc gcagttcgtc
     1981 ccagggaact gaaccacccc aaccgtgacg gccatatcag ctgcgctcca cgagatcgat
     2041 gacggcatcc tcaatgaccg ggttggtcaa gaatcgggcg cacatgtcct cgacctcgct
     2101 tcgagcggat gcctcgtcgc tcgcctcgac ctggaaacgg atgctctttc cgacgcgcac
     2161 cccactcact ccatcgaaac ccagtgcggg catcgcccgt tcaatcgtcg atccctgcgg
     2221 gtcggcgatg ccggggcgaa gctgcacatc cacgtgacag tcgaagatca tgttggaaac
     2281 tctttcacat tgacgggggc gacatggacc gtgattcggt ccgggccggt ttgaaccggg
     2341 aggatcaggc tccgggccac tcgctcagcc gccgaccgga gacccgttcg tacgcctcgg
     2401 cataacgctg gctcgatgca gcgaccacct caccgggaag ttgcgggggc ggcggagtct
     2461 tgtcccagtc gagggtctcg agatagtcgc gaaccggctg cttatcaaag ctcggagggg
     2521 tggttcccgg gatccacgaa tcgacgggcc agaaccgaga cgagtccggc gtcagcacct
     2581 catcagcgag cacgagttca ccatcgatga ttcccatctc gaacttcgtg tcggcaatga
     2641 tgatgccccg ttgcgccgcc agctcggaac cacgccgata caactcaatc gacaggtcgc
     2701 gagccttgct ggccaactcg gcgccgacga tgtcgaccgc ccgctcaaag ctgatgttct
     2761 cgtcgtggtc cccgacttcg gccttggtcg agggggtgaa taccggctcg gggagcttgt
     2821 ccgattcctg tagcccggcg gcgagccgct gtccgtgcat cgtggagtgg ttcttgtatt
     2881 ccttccatgc cgatccggtg atatatccgc gcacgatgca ctcgatcggc agcatctccg
     2941 ccttgcggac cagcatgaac cgtccggcga tttcatcgtt tcgggcgccc tccggcagat
     3001 cggcgagatc ggtggagatg agatgactcc cgacgagatc ggcgaactgt tcaaaccaga
     3061 acgccgacat tgcggtgagg accctgccct tgttgggaac gggctcgttc atgaccacgt
     3121 cgaaggcgct gatgcggtct gaagcgacca tgacccagcg cccgtcgccg gcgtcataga
     3181 tgtcgcggac cttgccggag tagatgtggg ggagaccgag atcaatcatg ggtggccctt
     3241 tctggggcgg gaggagaagc gactacagga tcggggccgg agcgtaggct gcggctgctg
     3301 ggtaacgacg tgccagggcc tcgacctggt cgacgaacgc cgcgctctgg gcgacggcgt
     3361 tgcccgtgaa acgcaacggt tccgcgaaga gcgcggcgag cgtccccggg gccaagccga
     3421 ggcgatcatc cgtctcgagg cgctcgacga ggtcgtttga accgtccagg tgttcgcgca
     3481 tcgccagagc tgcggccacg gcatgctcct tgatgacctc atgggcgacc tcgcgcccga
     3541 caccgtgttg aaccgccgcc atgagcacct tggtcgtggc gaggaatggc agatagcggt
     3601 cgagttcgcg ctggatcacc gccggatacg cgccgagttc gtcgagcacc gtcaacaacg
     3661 tctcgaaaag accatcgatg gcgaagaacg cgtcgggcaa cgccacgcgg cgcaccaccg
     3721 aacaactgac gtctccctcg ttccactggt ctcccgcgag gcctccgacc atcgtgagat
     3781 acccgttgag gatggtgtga aacccacaga tgcgttcgca gctgcggctg ttcatcttgt
     3841 gcggcatcgc cgacgaaccc acctggcccg gcttgaaccc ctcggtcaca agatcatgac
     3901 cggccatcaa gcgcatggtc gtcgccaggc tggagggagc cgccgcggcc tgcaccaatg
     3961 ccgcaacgac atcgaagtcg aggctacgtg ggtagacctg accgacgttg gtgagcacgt
     4021 gcccgaaccc gaggtgtgca gcaactcgag cttcgagttc acccatcgcc ctcgcatcgc
     4081 ctcccagcag atccatttga tcctgttggg tgcccaccgg gcctttcaat ccccgcagcg
     4141 ggtagcgctc caacaggtct tcgatgcggt cgaacgcgac gagcagctcc tggccggcgt
     4201 tggcaaagcg tttgcccagc gtggtcgtct gcgccgccac gttgtggctc cggccggtca
     4261 gcggctgctc ggcgaattcg ccgacccgac tgcccagctt cgcgagcgcg ctcaccattc
     4321 ggtcgcgcac caactcgagc gccaggcgga tctgaagctg ggcaatcggg tcggcgaatt
     4381 cgccgagcag ccgctgac
//
LOCUS       DAOOMA010000167         4373 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_24139_length_4373_cov_3.326077, whole genome shotgun sequence.
ACCESSION   DAOOMA010000167 DAOOMA010000000
VERSION     DAOOMA010000167.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 4373)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4373)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4373
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24139_length_4373_cov_3.326077"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            149..724
                     /locus_tag="PLP95_05770"
     CDS             149..724
                     /locus_tag="PLP95_05770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HPB45343.1"
                     /translation="MTSAPTIRPGTAPNTGLDDVAEIANVGEESEVDCSIARALGVIG
                     DRWTILIMRDAFRGIRRFDDFRTDLGIARPVLADRLKKLVEAGVMVRRQYQTHPQRFE
                     YRLTPMGMELSPALVALMRWGDRWLNDGSPPTVLVHEACGRELEQGFWCRTCKTTFTP
                     RDIASLHPDHDLSDHELSDRSPVATGDADDH"
     gene            714..3155
                     /locus_tag="PLP95_05775"
     CDS             714..3155
                     /locus_tag="PLP95_05775"
                     /EC_number="2.5.1.147"
                     /EC_number="4.3.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020379530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional FO biosynthesis protein CofGH"
                     /protein_id="HPB45344.1"
                     /translation="MTTESMTTESITTELLSLPLDELLKRAAAVRDRRYGTRVTYSPK
                     VFIPLTMLCQDKCGYCTFAQPPARLEAPYLTPEQVLDIARAGAAVGCHEALFTLGERP
                     ELRYPVAAQWLADHGYASTVEYLVAMCRLVRDEVGLLPHANAGALSRDELALLRPVSA
                     SQGMMLESLNADLDCHRGSPDKVPQRRLDTLEAAGELQIPFTTGILCGIGESRQDRVD
                     ALVAIAEAHRRWGHVQEVIVQNFLPKAGTAMHKSPPCPHDDYLEAIALARLILPEDIH
                     LQVPPNLTDDFGELLNAGIDDWGGVSPVTADHVNPERPWPALERLTDVTEVAGHVLAP
                     RLTIYPEFALDPERWLAEDMRFGVLDRSDAEGLGRDRDTATGDPSAEVWYSGADTEPP
                     LVVEAPNVSATGALAELMRPATAAAGSAVRGAVAEVLDGVRMGQVLGEDEIVTLFGAR
                     GPEVRAVAALADELRREIVGDEVTWVSNRNINYTNVCTFKCRFCGFSKGPLSLNLRGN
                     PYNLSLEEIAERVVEAKALGATEVCLQGGIHPKFDGDYYLAVTRIVHEAVPDMHIHGF
                     TALEVTEGAKRNQEPLADYLRRMKVAGLKTLPGTAAEILDDEVRQVLCADKINTEEWL
                     HAHRTAHEVGLRSNVTIMFGSIEHPRSWARHLLITRALQAETGGFTEFVPLPFVHMAS
                     PIYLQRASRRGPTFREVVLMHAIGRIVYRGAIDNVQASWVKLGLGGVRQLLRAGVNDL
                     GGTLINENISRAAGAEHGQMATEADFRALVEPLGRRLVQRTTLYGRVDDAGHARIDTE
                     ASRNTGLNSALVAGA"
     gene            3152..4198
                     /locus_tag="PLP95_05780"
     CDS             3152..4198
                     /locus_tag="PLP95_05780"
                     /inference="COORDINATES: protein motif:HMM:TIGR00730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HPB45345.1"
                     /translation="MSEQRHRRNPPPRTGDPTADAAVRDLVAVSGVQSDNDLLTEMLG
                     NVVRLCRDGADRGELKLISLALRDLRRASAQFKPYIGQPKCSVFGSARTKPGEPAYTA
                     AVELGAALAADDWMVITGGGPGIMTAAVEGAGADNSFGVTIRLPFEPMEGGGIVPDER
                     LVRFRHFFSRKLTFMRQSAAYVVFPGGYGTLDETFELLTLIQTGKVTPAPVVLFEPDG
                     DGYWKRWRDFIEYELEDASLISPDDVDLVFITSSVADAVRYLDEFYRVYHSMRWVGDQ
                     LVIRLRRQLTDEELAAVNREYADLVVDGAIERCDPLAPEVDDADLLHLPRLVMKFDQR
                     SFGRLHQMVRSLGR"
BASE COUNT          730 a         1470 c         1434 g          739 t
ORIGIN      
        1 gttctccgat aaccagtggt cgtgatcacg accactggtt atcggagaac gggcgtttgg
       61 ggtggtttgc gggcgcttgg gctggttgtt gggggtgggt ttgtgaaacg aacccactcg
      121 gttcgctttt cgaactatgg tgcggcccgt gacttcagct cccaccattc ggccgggtac
      181 cgccccgaac accggcctcg acgacgtcgc cgagatcgcg aacgtcggcg aggaatccga
      241 ggttgattgt tcgatcgctc gcgccctcgg ggtgatcggc gatcgttgga cgatcctgat
      301 catgcgcgat gcctttcgcg gcatccgccg attcgatgac ttccgcaccg acctgggcat
      361 cgctcggccg gtgctggcgg atcggctcaa gaagctcgtc gaagccgggg tgatggtgcg
      421 gcgccaatac cagacacatc cgcagcgctt cgagtaccgc ctcaccccga tgggtatgga
      481 actcagcccg gcgctcgtcg cgctcatgcg ctggggcgac cgttggctca acgacgggtc
      541 gccgccgacg gtgctcgtgc atgaggcctg cgggcgcgaa ctggaacagg ggttctggtg
      601 ccggacctgc aagacgacct tcacccctcg cgacattgct tcgctccacc ccgaccacga
      661 cctgtccgac cacgaactgt ccgaccgctc ccctgtcgcc accggagacg ccgatgacca
      721 ctgagtcgat gaccaccgag tcgatcacga ctgaacttct ttccctgccg cttgacgaac
      781 tcctcaagcg cgctgcagcg gtgcgcgacc gacgctacgg aactcgggtg acctattcgc
      841 cgaaggtgtt cataccgctg acgatgctgt gtcaggacaa gtgcgggtac tgcaccttcg
      901 cccagccgcc ggcccggctc gaggcgccct acctcacccc cgaacaggta ctcgacatcg
      961 cccgagccgg agcggcggtc ggatgccacg aggcgctgtt caccctcggc gagcggcccg
     1021 agttgcgcta tccggtggcc gcacagtggc tcgccgatca cgggtacgcg tcgacggtcg
     1081 agtacctggt ggcgatgtgt cggttggtgc gagacgaggt cgggctcctg ccgcatgcga
     1141 acgcgggtgc attgagccgg gacgaactgg cgctgctgcg gcccgtttcc gcaagccagg
     1201 gcatgatgct cgaatcgctc aacgccgacc tcgattgcca ccgaggatcg ccggacaagg
     1261 tgccacagcg tcgtctcgac accctcgagg cggcgggcga actgcagatc ccgttcacga
     1321 ccggaatcct gtgcggcatc ggcgagtccc gtcaggaccg cgtcgatgcg ctcgtggcga
     1381 tcgcggaggc ccatcgccgc tggggccatg tccaggaagt gatcgtgcag aatttcctgc
     1441 caaaggccgg caccgcgatg cacaagtcgc cgccctgccc tcacgacgac tacctcgaag
     1501 ccatcgcgct cgcccggttg atcctgcccg aggacatcca cctgcaggtg cctccgaacc
     1561 tcaccgacga cttcggtgaa ctgctgaacg ctggcatcga cgactggggc ggcgtgtcgc
     1621 cagtgacggc ggaccacgtc aatcccgagc gaccgtggcc ggcgctcgaa cgtctcaccg
     1681 acgtcaccga ggtggcggga cacgtcctcg ctccgaggct cacgatctac cccgaattcg
     1741 cgctcgatcc cgagcggtgg ttggccgagg acatgcgctt tggcgtcctc gaccgcagcg
     1801 acgccgaggg cctcggccgc gaccgcgaca ccgccacggg cgatccctcc gccgaggtct
     1861 ggtattcggg ggccgacacc gaaccgccac tcgtcgtcga ggccccaaat gtgtctgcga
     1921 ccggcgcact cgccgagttg atgcgaccag ccaccgccgc cgctggctcg gcggttcggg
     1981 gcgcggtcgc cgaagtgctc gacggggtac ggatgggtca ggtgctcggc gaggacgaga
     2041 tcgtcaccct gttcggcgcg cgtggtcccg aggtgcgtgc cgtcgccgcc ctggccgacg
     2101 agttgcgccg cgagatcgtc ggcgacgagg tcacctgggt ctcgaaccgc aacatcaact
     2161 acacgaacgt atgcaccttc aagtgccgct tctgcggatt ctccaaaggc ccgctgtcgc
     2221 tcaacctgcg cggcaacccc tacaacctct cgctcgagga gatcgccgaa cgcgtcgtcg
     2281 aagccaaggc gctcggcgcc accgaggtgt gcctccaggg cgggatccac ccgaaattcg
     2341 acggcgacta ttacctggcg gtcaccagga tcgtgcacga ggccgtgccg gacatgcaca
     2401 ttcatgggtt caccgcgctc gaggtcaccg agggggccaa gcgcaaccag gaacccctgg
     2461 ccgactacct gcgccgcatg aaagtagccg ggctcaagac cctgcctggc accgcggcgg
     2521 agatcctcga cgacgaggtc cgccaggtgc tctgcgccga caagatcaac accgaggaat
     2581 ggctgcatgc ccaccgcacg gcccacgagg tggggctgcg gtcgaacgtg acgatcatgt
     2641 tcggttcgat cgaacacccc cgaagctggg cccggcacct gctgatcacc cgggcgttgc
     2701 aggccgagac gggcggattc accgagttcg tgccgctgcc gttcgtccac atggcctcgc
     2761 cgatctacct gcagcgggcc tcacggcggg gcccgacctt ccgggaggtg gtgttgatgc
     2821 acgccattgg acgcatcgtg taccggggag cgatcgacaa cgtgcaggcc tcgtgggtga
     2881 agctcggcct tggcggggtg cgccagttgt tgcgcgccgg agtgaacgac ctgggaggaa
     2941 cgctgatcaa cgagaacatc agccgagcgg cgggtgctga acacgggcag atggccaccg
     3001 aggccgactt ccgggcactg gtcgaaccgc tcggtcgtcg actggtccag cgcacgaccc
     3061 tttatggacg cgtcgacgac gctggccatg cccgcatcga caccgaagcg tcgcggaaca
     3121 ccgggttgaa cagcgcactg gtggccggcg catgagtgag caacgtcatc gccgcaatcc
     3181 acctccccgc accggcgatc cgaccgccga tgccgccgtc cgcgacctgg ttgcggtcag
     3241 cggggtgcag tctgacaacg acctgctcac cgagatgctg ggcaatgtgg tgcgcctgtg
     3301 ccgagacggt gcggaccggg gcgagttaaa gctgatcagt ctcgctctgc gcgacctccg
     3361 ccgcgcctcg gcccagttca agccgtacat cggtcagccg aagtgttcgg tgttcggctc
     3421 ggcgaggacg aaaccgggtg aacccgcgta caccgctgcg gtcgaattgg gggcggcgct
     3481 cgccgccgac gattggatgg tcatcaccgg cggtggcccc ggcatcatga ccgccgcggt
     3541 ggaaggtgcc ggtgctgaca acagcttcgg ggtcacgatc cgccttccgt tcgaaccgat
     3601 ggagggcggc ggcatcgtgc ccgacgaacg cctggttcgg tttcggcact tcttcagccg
     3661 caagctgacc ttcatgcgtc agtcggccgc gtacgtcgtg ttccccggtg gttacggcac
     3721 cctcgatgaa accttcgagt tgctgacgct gatccagacc ggcaaggtga ccccggctcc
     3781 ggtggtgttg ttcgaaccgg atggcgacgg ctactggaag cgttggcgcg acttcatcga
     3841 atacgaactt gaggacgcat cgctgatctc ccccgacgat gtcgacctcg tgttcatcac
     3901 ctcgtcggtc gccgacgccg tgcgatatct cgacgagttc taccgcgtct atcactcgat
     3961 gcgctgggtg ggcgaccaac tcgtcatccg gctgcgccgt cagctgaccg acgaggaact
     4021 cgcggcggtc aaccgcgagt acgccgacct ggtcgtcgac ggggcgatcg agcgatgcga
     4081 cccgttggca cccgaggtcg acgacgcaga cctgctgcac ctgccccggt tggtgatgaa
     4141 gttcgatcaa cgcagtttcg gacggctcca tcagatggtg cgctcgctgg gccgctgaca
     4201 aaccgggccg agcagcgcac tgggccgagc agcgcgctgg gcttcaccaa tcggtggcgg
     4261 cagaccctga gcccccgctc tcgagcagat tggccgcctt gagcaccgag cgtcgagccc
     4321 ggccgagcag cttgtcgtcg acgggtagtt catcgcctcc ggcgtcgatg gcg
//
LOCUS       DAOOMA010000168         4360 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_24224_length_4360_cov_3.860163, whole genome shotgun sequence.
ACCESSION   DAOOMA010000168 DAOOMA010000000
VERSION     DAOOMA010000168.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 4360)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4360)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4360
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24224_length_4360_cov_3.860163"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..238
                     /locus_tag="PLP95_05785"
     CDS             <1..238
                     /locus_tag="PLP95_05785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020379136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HPB45346.1"
                     /translation="RLDGIDHGPEGSVDKLLMTQVEQAVGHASTSIGGLGHGGDDDTW
                     LKVYLYSRAQSVMGGTSQIQRNLIAQRILGLPTK"
     gene            235..1056
                     /locus_tag="PLP95_05790"
     CDS             235..1056
                     /locus_tag="PLP95_05790"
                     /inference="COORDINATES: protein motif:HMM:NF027439.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HPB45347.1"
                     /translation="MTDTSTTHSVSDNYGLPAEIQVEAHGTIRVVRLNRPEALNASNH
                     VLHEAIADLFERLGADPDITAIVLTGNGRAFSAGGDFEYLDELTKDDALREQTLSSGR
                     RIVTGMVACRPPIIAAVNGPAVGLGCSLVALSDIVFMAESAHLADPHVALGLVAADGG
                     PVVWPLLTSMQLAKEYAITGDRIPAKRAAEIGLANHVCPDGEVFDQAMACATRISKLP
                     KRTVQDTKRVMNLHLERAVLGCLDYALAAEDRSFRSPELRANLDHLKAKQDAASK"
     gene            complement(1199..2185)
                     /locus_tag="PLP95_05795"
     CDS             complement(1199..2185)
                     /locus_tag="PLP95_05795"
                     /inference="COORDINATES: protein motif:HMM:NF012656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HPB45348.1"
                     /translation="MDGDDRELFERSLRHAAQTCSGAELDRALIELGWIEALEFDERL
                     AVSAWFETQGASTTSSGALELVLMHALGLHDIGSDTALVLPQLGSTSVPGQVVEGRLN
                     VHGLVLGSPGADGHDRHSSYVVVWNTGSTTVAHLATSAAVTLRPISGIDPALGLLRVR
                     GESMTPDDAPRLDTDPLEWGRAVAAAQVALGHELVGASRTMLAQAVEHALGRIQFGRP
                     ISQFQAVRHRLAEVLIAIETADAMLDAAWQDRTATSAAMAKSLSGRAARLAARNCQQV
                     LAGVGFTTEHPLHLTIRRVLVLDELLGSARCLTRELGDSLIESRALPPLPLL"
     gene            complement(2241..3371)
                     /locus_tag="PLP95_05800"
     CDS             complement(2241..3371)
                     /locus_tag="PLP95_05800"
                     /inference="COORDINATES: protein motif:HMM:NF014790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HPB45349.1"
                     /translation="MELEEFREGLDAWLDANAALLAAHHEGAGTLDEQMAHLSQVKRE
                     TFDAGWMRYGWPTEVGGLGGSTLLRAYLGEALAARDLVEPGIYSMTEVLAPTMIDYAS
                     PELAAQMVPRLLRGDETWCQGFSEPDTGSNLASLACRGVRSDDGWHVTGQKVWTSLAQ
                     YAQRCVLLVRTGTVESAHRGITALFVDMDTPGITVRPIETMHGVEEFSEVFFDDVVVP
                     FDRMLGEEGQGWAVAMDLLPFERSTALWHRAAFLHQRLRQILDSAGPGTLDPVRVGEV
                     TQLLYAFRSRSRSTQYRLHDGERLGAETSIDKVLVATVEQAVFDLAEDGLAESITIGD
                     DPLSARWRSEFLYSRAATIYGGSAEIQRNIIARRLLDLGNDL"
     gene            complement(3381..3878)
                     /locus_tag="PLP95_05805"
     CDS             complement(3381..3878)
                     /locus_tag="PLP95_05805"
                     /inference="COORDINATES: protein motif:HMM:NF024966.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HPB45350.1"
                     /translation="MESPSPDTADLHNVIGLHDVIEVQQLLARYAVGMTKNDVESVMD
                     VFTSDGTYSAFGDTYVLADFPTLVAAAPKGLFMVGLPVVDLDAGAGTGTGEQPLCFVD
                     QTNHHMRIGWYTDTYLRTERGWRLRTRSMTFLRKSGARDSGRAHDPTRPTPTAASGSA
                     DATAG"
     assembly_gap    3940..3949
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            4024..>4360
                     /locus_tag="PLP95_05810"
     CDS             4024..>4360
                     /locus_tag="PLP95_05810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB45351.1"
                     /translation="MNGRDPGAERRRWNLRTVPEALARRYREEGHWDDSTLGATVAEG
                     LARRSRLEFNVHSAVRPWSGTFGEVDRMARTLAGSFRRAGVGPGDVVMFQMPNWLEAG
                     VTFWASAYLG"
BASE COUNT          716 a         1386 c         1536 g          712 t
ORIGIN      
        1 ccgcctcgac ggcatcgacc atggccccga ggggtcggtc gacaagttgt tgatgaccca
       61 ggtcgaacag gcggtgggtc acgcctcgac gtcgatcggc ggactcggtc acggcggaga
      121 tgacgacacc tggctgaagg tgtatctcta cagccgcgcc caaagcgtca tgggcggaac
      181 ctcacaaatc caacgaaacc tgatcgctca acgaatcctg ggactgccaa cgaaatgacc
      241 gacacatcca cgacccattc cgtctccgac aactacggcc tgcccgcgga gattcaggtg
      301 gaggcgcacg gcacgatccg cgtcgtgcgg ctgaatcgtc ccgaagcgct gaacgcgtcg
      361 aatcacgtcc tgcacgaggc catcgccgat ctgttcgaac gcctcggcgc cgaccccgac
      421 atcaccgcga tcgtgttgac cggcaacggt cgtgcgttct cggcaggcgg cgactttgag
      481 tacctcgacg aactcaccaa agacgacgca ttgcgtgagc agaccctgag ttcggggcga
      541 cgcatcgtca ccggcatggt ggcgtgtcgg ccgccgatta tcgccgcggt caacggcccg
      601 gcggtggggc tggggtgcag cctggtggcg ctcagcgaca tcgtgttcat ggccgagagc
      661 gcgcatctcg ccgacccgca cgtcgcgctc ggcctggtgg ccgcggacgg tgggcccgtg
      721 gtgtggccgc tgctgacgag catgcagttg gccaaggaat acgccatcac cggcgatcgc
      781 attccggcca aacgggccgc ggagatcggc ctcgccaatc acgtgtgccc cgacggcgag
      841 gtgttcgacc aggcgatggc gtgtgcgacg cgcatctcga agttgcccaa acgaacggtg
      901 caggacacca agcgggtgat gaacctgcac ctcgaacgcg ccgtgctcgg gtgcctcgac
      961 tacgcgctcg cggcggagga ccggtcgttt cgttcccccg agcttcgagc caacctcgat
     1021 cacctcaagg ccaaacagga cgccgcctcc aaatagggca ccagcccgcc ggcccgccgg
     1081 cactgcccgc ctcgcccgct tccctcgggt tctgtaggta attccggtgg ctatggccac
     1141 cgcaattacc tacagaacgc tcggtgggca gaacgcccgg tggacagggc gggcgggcct
     1201 acagaagcgg gaggggtggc agtgcgcggg actcgatgag cgagtcgccg agttcgcggg
     1261 tcaggcagcg ggccgacccg aggagttcgt cgagcaccag cacccgtcgg atcgtgagat
     1321 gcagcggatg ctcggtggtg aatccgaccc cggcgagtac ctgctggcag ttgcgggccg
     1381 cgagtcgtgc cgcccgtccg gagagggact tggccatggc cgcggaggtt gccgtgcggt
     1441 cctgccaggc ggcgtcgagc atcgcgtcgg cggtttcgat ggcgatgagc acctcggcga
     1501 ggcggtggcg caccgcctgg aactgcgaga tggggcgccc gaactggatc cggccgagcg
     1561 cgtgctcgac ggcctgggcg agcatcgttc gtgaggctcc gaccagttcg tggccgagcg
     1621 ctacctgagc ggcggccaca gcgcgccccc actcgagcgg gtcggtgtcg aggcgtggcg
     1681 cgtcatcggg ggtcatcgac tcgccacgca cccgaaggag gccgagcgcc gggtcgatgc
     1741 ccgagatcgg ccgcagcgtc accgcggcgc tggtcgccag atgcgcgacc gtggtcgagc
     1801 ccgtgttcca gacgacgaca taggagctgt ggcgatcgtg cccgtcggcg ccgggggatc
     1861 cgaggacgag gccgtgcacg ttcagccggc cctcgaccac ctgcccgggc accgaggtgg
     1921 atccgagttg gggaagcacg agtgcggtat cggatccgat gtcgtggagt ccgagcgcgt
     1981 gcatgaggac gagttcgagg gcgcccgaac tcgtggtcga ggcaccctgg gtctcgaacc
     2041 atgcggacac ggcgagccgt tcgtcgaact cgagggcctc aatccagccg agctcgatga
     2101 gggcccggtc gagttcggcc cccgaacagg tctgggccgc gtggcgaagg cttcgctcga
     2161 agagttcgcg gtcgtcacca tccatcagca gcacctcacg atcagcgccc tcagcagcgc
     2221 cccggtcagc agcaccccgg tcacagatcg tttccgaggt cgaggagtcg gcgggcgatg
     2281 atgtttcgct gaatctcggc gctgccgccg tagatcgtcg ccgcccggga gtacaggaac
     2341 tcgctgcgcc atcgagcgct gagcgggtcg tcgccgatcg tgatcgactc ggccagtccg
     2401 tcctccgcga ggtcgaatac ggcctgttcg acggtggcga ccagcacctt gtcgatggag
     2461 gtttccgcgc cgaggcgttc gccgtcatgc agtcggtact gcgtcgaacg cgaccgggat
     2521 cggaaggcgt agagcaactg ggtcacctca ccgacccgca ccggatcgag ggtgccggga
     2581 ccagcgctgt cgaggatctg gcggagccgc tgatggagaa aagccgctcg atgccacagc
     2641 gccgtgctgc gttcaaacgg caacaggtcc atggccacgg cccagccctg gccctcctcg
     2701 ccgagcattc tgtcgaaggg gacgacgaca tcgtcgaaga acacctcgga gaattcctcc
     2761 acgccgtgca tcgtctcgat cggccgtacg gtgatcccag gggtgtccat gtcgacgaac
     2821 agcgcggtga tgccacgatg ggccgattcg acggttccgg tccgcaccaa caacacgcag
     2881 cgctgcgcgt actgggcgag gctcgtccac accttctggc cggtgacgtg ccagccgtcg
     2941 tcgctgcgaa cgccacggca cgcgagcgat gcgaggttcg atccggtgtc gggctcggag
     3001 aacccctgac accacgtctc gtcgcctcgc aacaagcggg gcaccatctg cgccgccagt
     3061 tcgggcgagg cgtagtcgat catcgtcggg gccagcacct cggtcatcga gtagatgccg
     3121 ggttcgacga ggtcgcgcgc tgcgagtgct tcgccgaggt aggcgcgcag cagcgtcgat
     3181 ccgccgaggc cgccgacctc ggtgggccag ccgtagcgca tccatccggc gtcgaaggtt
     3241 tcgcgtttga cctgtgacag atgggccatc tgctcgtcga gtgtgcccgc tccctcgtgg
     3301 tgggcggcga gcagtgcggc attggcgtcg agccaggcgt cgaggccctc gcgaaactcc
     3361 tcgagttcca cggccgccgc tcagccggcc gtcgcgtcgg cggagccgga cgcggcggtc
     3421 ggggtcggtc gggtggggtc gtgtgctcgc ccggagtctc gtgccccgct cttgcgcagg
     3481 aacgtcatcg aacgggtgcg gaggcgccac ccccgctcgg tgcgcaggta ggtgtcggtg
     3541 taccagccga tgcgcatgtg gtggttggtc tgatcgacga aacacagcgg ctgttcgccg
     3601 gtgccggttc cggccccggc gtcgaggtcg accacgggaa gcccgaccat gaagagcccc
     3661 ttgggtgcgg cggcgaccaa cgtcgggaag tcggcgagta cgtaggtgtc tccgaacgcg
     3721 ctgtaggtgc catcggaggt gaacacgtcc atcaccgact cgacgtcgtt cttggtcatg
     3781 ccgaccgcgt aacgggcgag cagttgctgc acctcgatga catcgtgcag gccgatgacg
     3841 ttgtggaggt cggcagtgtc gggggagggt gactccataa cgtgacgata accaagttct
     3901 cgcattcgga caacgacctt tccggtaatg aggtggccgn nnnnnnnnnc gtaggatgtg
     3961 cgcttgagtt gacgctagac gaggcgaatc gccgttcgcg gtgattgtcg cgagggggca
     4021 gaggtgaacg gtcgcgatcc gggtgctgag agacgacgtt ggaacctgcg aaccgttcct
     4081 gaagcgttgg ctcgtcgcta tcgcgaggaa ggccactggg atgactcgac cctgggcgcc
     4141 acggtggcgg aggggctggc gaggcggtcg cgtctggaat tcaacgtcca ctcggcggtt
     4201 cggccatggt cgggaacctt cggcgaggtc gaccgcatgg ctcgcacgct ggccgggtcg
     4261 tttcggcgtg ccggggttgg cccgggcgat gtggtcatgt ttcagatgcc gaactggctt
     4321 gaagccgggg tgaccttttg ggcctcggcg tatctcggtg
//
LOCUS       DAOOMA010000169         4338 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_24390_length_4338_cov_4.234882, whole genome shotgun sequence.
ACCESSION   DAOOMA010000169 DAOOMA010000000
VERSION     DAOOMA010000169.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 4338)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4338)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4338
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24390_length_4338_cov_4.234882"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..926
                     /locus_tag="PLP95_05815"
     CDS             <1..926
                     /locus_tag="PLP95_05815"
                     /inference="COORDINATES: protein motif:HMM:NF015155.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HPB45352.1"
                     /translation="SHRPVVANPGSATISGALAELPGVSQTTPPLPNEEGTAAIVQVT
                     PDSGPEDEATEDLVRAIRDQADLEESTGARIDVTGATAFYIDISQRLNDRLPIFIAAV
                     VSLSFLLLTAVFRAPVVAAKAAVMNLLGIGAAYGVVVAVFQWGWAKDLIGLEQTVPII
                     SFLPMGLFAVLFGLSMDYEVFILSRIREAWIVDHDNESAIVHGLSASARVITAAAAIM
                     FAVFASFVAGDSAEIKMFGLGLAVAVLLDATVIRMLVVPASMKVLGNANWWIPNWLDR
                     LLPNLDIEGSEADEELDPPPPTPHPTETASA"
     gene            923..1636
                     /locus_tag="PLP95_05820"
     CDS             923..1636
                     /locus_tag="PLP95_05820"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF015048.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HPB45353.1"
                     /translation="MNLDEPPAQLAARTALASAMQRLGHSLVAHQVDTDLAVELAEVA
                     ERYTSTVQRGAPRDRATEMLSSPRVTAALNGKRAIIDDGAEIDLFRDSFVSGRTNPMG
                     IGVRVVRDGDTAVATTTLGPAFEGAPGRAHGGAVGAILDETMGYLLPIIGEMAYTANL
                     SIDYIAPAPLGVEVSFTARLRDRADRKLWIEAVGESADGVFVRAEALFLTVDLSRFTN
                     ASAEGDSVNAADAQAVELP"
     gene            1633..2529
                     /locus_tag="PLP95_05825"
     CDS             1633..2529
                     /locus_tag="PLP95_05825"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HPB45354.1"
                     /translation="MTVAARPERFDLPGADLRLAADRWGPRTDDVVVFLHGAGQSRRA
                     WDDAAEAVAAIGWHAITVDHRGHGDSEWPIEADYDWDRFADDVIAIVEHLGTPPVLVG
                     ASLGGMSALLAQARTDEQLFRAVVLVDVTPRMELGGVQRIVGFMSAHPDGFDSLDAAS
                     AIIAEYTGRPRPPTPDGLRQVLAHGSDGRWRWRWDVRFLEGRADMIIGGDIEALREIQ
                     VRERLHEGARRIKVPALVVRGAESDLVSTEGVREFVDAVPGAQYVDVADAGHMVAGDQ
                     NDTFTAAVLTFLSEVRTGTTRR"
     gene            2526..3419
                     /locus_tag="PLP95_05830"
     CDS             2526..3419
                     /locus_tag="PLP95_05830"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HPB45355.1"
                     /translation="MTTDPAAPTVHRIPTRNGDELRVLDWGGSGPPVVLLHPNGFCGG
                     LYEPVAATLLGTARAVAIDLVGHGGSTTPTDPTRYRFASMANDVLDVLDHLDLCGIAG
                     VGGSLGGAVAVLVDQLDQGRWSQLLLAEPVAFPPTLFRTTTPNPMAVAARRRRRLFAT
                     RAEMHDQYCDKAPLSQLSDDALDAYLRWGTVDCGDGVHLACDPDTEALIFEMSATSYG
                     APAAWEHLEHLACPAVIVAGEDTFLPAIFDQQAERANATLLTVPGGHFVLHEDTRRTE
                     ALIREHVLTQPAPSPATGGSR"
     gene            3416..>4338
                     /locus_tag="PLP95_05835"
     CDS             3416..>4338
                     /locus_tag="PLP95_05835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB45356.1"
                     /translation="MTTRHRYTRDELMSDPPYAERIERNGVRFHGGYDTEGTYLPPRS
                     LHRAAAIDAWTRQLEAAGHPTSVVDTDAVKRSFVPNATQAKMLLSNDATGAMTRILTL
                     IGVVEGFGNDGIKLIPVVDLAEHIVEPTDDTCVGHLFNGLLEAHGNDEAGTDGEAGHD
                     QMWFAVRDAALADPPVTLDMFQELPIAPPPGYSGPASPAAEAISVGSMIGGMRDDVSP
                     ELQLLVRAMVQILVIELLAYHTFAWASDVLGDPSCSHDAAFARTTIDHIRADEDLHVG
                     YLQCALAELATMTVRRTDGGTVRGADLVVAAS"
BASE COUNT          757 a         1586 c         1344 g          651 t
ORIGIN      
        1 tgagccacag accagtcgtg gcgaatcctg gatccgccac gatctcggga gcgctcgcgg
       61 agctcccagg tgtcagtcag acgacgccgc cgctgcccaa cgaggagggc acggcggcca
      121 tcgtgcaggt gacgcccgac agcggccccg aggacgaagc cacagaggac ctggtccgtg
      181 ccatccggga ccaagccgac ctcgaggaga gcaccggcgc tcgcatcgac gtcacgggtg
      241 ccacggcgtt ctacatcgac atctcccagc ggctcaatga tcgcctgccg atcttcatcg
      301 ccgccgtggt gtcgctgagc ttcctgctgc tcaccgcagt gttccgagcg ccggtcgtcg
      361 cagcgaaggc tgcggtcatg aacctgctcg gcatcggcgc ggcctacggc gtcgtcgtgg
      421 cggtcttcca atggggatgg gccaaagatc tcatcgggct ggaacagacc gtgcccatca
      481 tctcgttcct tccaatggga ctcttcgcgg tgctgttcgg cctctcgatg gactacgagg
      541 tgttcatcct gtcgcgcatc cgagaggcgt ggatcgtcga ccacgacaac gagtcggcca
      601 tcgtccacgg actctctgcc agcgcgcgcg tcatcaccgc ggccgcagcg atcatgttcg
      661 cggtgttcgc cagtttcgtc gccggcgaca gcgccgagat caagatgttc gggctcggcc
      721 tcgccgtggc ggtcctgctc gacgccaccg tcattcgcat gctcgtcgtg ccggcgtcga
      781 tgaaggtcct gggcaacgcg aactggtgga tcccgaactg gctcgaccgg ctgctgccca
      841 acctggacat cgagggctcc gaagccgacg aagagctcga ccccccgcca ccgacacctc
      901 accctacgga gacagcatca gcgtgaacct cgacgaacca ccagcacaac tcgccgcccg
      961 cacagcgctc gcgtccgcaa tgcagcgatt gggccactcg ctcgtcgccc accaggtcga
     1021 caccgacctg gcggtcgagc tcgcagaggt cgccgagcgc tacacctcca ccgtgcagcg
     1081 tggtgcaccg cgggaccggg ccaccgagat gctctccagt cctcgcgtca ccgcagcgct
     1141 caacgggaag cgagccatca tcgacgacgg tgcggagatc gacctgttca gggactcgtt
     1201 cgtctccggg cggaccaacc ccatgggcat cggagtccgg gtcgttcgcg acggcgacac
     1261 cgccgtggcc accaccaccc tcggaccggc gttcgagggg gcgcccggcc gagcccacgg
     1321 cggcgccgtc ggcgccatcc tcgacgagac catggggtac ctgttgccga tcatcggcga
     1381 gatggcctac accgccaact tgtccatcga ctacatcgca ccagcaccgc tgggtgtcga
     1441 agtgtccttc accgcgcggc ttcgcgaccg ggcggaccgc aagctgtgga tcgaagccgt
     1501 cggcgaatcc gccgatggcg tgttcgtgcg agctgaagcg ctgttcctca ccgtcgacct
     1561 gtcgcgcttc accaatgcgt ccgccgaagg cgacagcgtc aacgcggcgg acgcccaggc
     1621 ggtggagctt ccatgaccgt cgctgcgcgc cccgagcggt tcgacctccc gggcgccgac
     1681 ctgcgattgg ccgcggaccg ctggggtccc cgcaccgacg atgttgtggt gttcctgcac
     1741 ggtgcgggcc agagtcgccg agcatgggac gacgccgcag aagcggttgc agcaatcggc
     1801 tggcacgcca tcaccgtcga ccacaggggc catggtgaca gcgaatggcc gatcgaggcc
     1861 gactacgact gggaccgctt cgccgacgac gtgatcgcca tcgtcgagca cctcggcacg
     1921 ccacccgtcc tcgtcggcgc gtcactgggc gggatgtcgg cgctgttggc gcaggctcga
     1981 actgacgaac agctcttccg agcggtggtt ctggtcgatg tcaccccgcg gatggagctc
     2041 gggggcgtac aacgcatcgt cggtttcatg tcagcgcacc ctgacggctt cgactccctc
     2101 gacgccgcgt cagcgatcat cgctgagtac accgggcgac ctcggccgcc gacaccggac
     2161 ggtctgcgcc aggtcctcgc tcatggctcc gacggccgct ggcggtggcg ctgggacgtc
     2221 cgcttcctcg aaggacgcgc cgacatgatc atcggcggcg acatcgaagc gttgcgggag
     2281 atccaggtgc gcgaacggct ccacgaaggt gctcgccgca tcaaggttcc cgcgctcgtc
     2341 gtgcgcggcg ccgagagcga cctcgtctcc accgaaggcg tccgagagtt cgtcgacgcg
     2401 gtaccgggcg cccagtacgt cgacgtggcc gacgccggtc acatggtcgc cggggaccag
     2461 aacgacacct tcaccgcagc ggtcctcacg ttcctgtccg aagtgcgcac gggcacgacc
     2521 agacgatgac gacggacccc gctgcgccga ccgtgcaccg gatccccaca cgcaacggcg
     2581 acgagctgcg cgtgctcgac tggggcggca gcggaccacc tgtggtgcta ctgcacccga
     2641 acgggttctg cggaggactc tacgaaccgg tcgccgcaac gctgctcggg accgcccgag
     2701 cggtcgcgat cgacctggtc ggccacgggg gcagcacgac accaacagac ccgacgcgct
     2761 accggttcgc gtcgatggcc aacgacgtgc tcgacgtgct cgaccacctc gacctctgcg
     2821 gcatcgccgg cgtcggcggg tcactcgggg gagcagttgc ggtcctcgtc gaccagctcg
     2881 accaagggcg gtggtcacag ctcctgctcg ccgaaccagt cgcgtttccc ccaacgctgt
     2941 tcagaaccac gacgcccaac ccgatggcgg tcgctgctcg tcgccgccgc cggctattcg
     3001 ccacccgagc cgagatgcac gaccagtact gcgacaaggc gcccctgtcc caactcagcg
     3061 acgatgcgct cgacgcgtac ctgcgatggg ggaccgtcga ttgcggcgac ggtgtgcatc
     3121 tcgcgtgtga ccccgacacc gaagcgctca tcttcgagat gtctgccact tcgtacggcg
     3181 cccccgccgc ctgggaacac ctcgagcacc tcgcatgccc cgctgtgatc gtcgccggag
     3241 aggacacctt cctgcccgcc atcttcgacc agcaggccga acgggccaac gccacattgc
     3301 tcaccgttcc cggcgggcac ttcgtgctgc acgaggacac ccgccgtacc gaagcactca
     3361 tcagagagca cgtgctcacc caaccagccc catccccagc aaccggagga tcccgatgac
     3421 cacacgacat cgatacaccc gcgacgagct catgagcgac cctccctacg ccgaacgcat
     3481 cgagcgcaac ggagtccgct tccacggcgg ctacgacacc gaaggcacct acctgccacc
     3541 caggtcgctg caccgagcag cagccatcga cgcatggaca cgccagctcg aggccgcagg
     3601 acacccgacc agcgtcgtcg acaccgacgc ggtcaaacgg tcgttcgtcc ccaacgccac
     3661 ccaggcgaaa atgctgctga gcaacgacgc gaccggcgcc atgacccgga tcctgaccct
     3721 gattggcgtc gtcgaggggt tcggcaacga cggcatcaaa ctcatccccg tcgtcgacct
     3781 cgccgagcac atcgtcgaac caaccgacga cacctgcgtc ggtcacctgt tcaacggcct
     3841 gctcgaagca cacggcaacg acgaagcagg aacggacgga gaagcaggac acgaccagat
     3901 gtggttcgcc gtgcgcgacg cagcgctcgc cgacccgccc gtcacgctcg acatgttcca
     3961 ggagctcccg atcgccccgc ctcccggcta cagcggcccc gcaagcccag ccgcagaagc
     4021 gatcagcgtc ggatcgatga tcggcggaat gcgcgacgac gtctcccccg agctgcagtt
     4081 gctggtgaga gccatggtgc agatcctcgt gatcgagctg ctcgcctatc acaccttcgc
     4141 ctgggcctcg gatgtgctcg gcgacccgtc gtgcagccat gacgccgcgt tcgcacgcac
     4201 gaccatcgac cacatccgcg ccgacgagga cctccacgtc ggctacctgc aatgcgccct
     4261 agcggaactt gccaccatga ccgtacgtcg caccgacggc ggaaccgtcc gtggtgccga
     4321 tctcgtggtc gccgcatc
//
LOCUS       DAOOMA010000170         4328 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_24473_length_4328_cov_4.643576, whole genome shotgun sequence.
ACCESSION   DAOOMA010000170 DAOOMA010000000
VERSION     DAOOMA010000170.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 4328)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4328)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4328
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24473_length_4328_cov_4.643576"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..772
                     /locus_tag="PLP95_05840"
     CDS             <1..772
                     /locus_tag="PLP95_05840"
                     /inference="COORDINATES: protein motif:HMM:NF012488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HPB45357.1"
                     /translation="RYGRVSPDELLDAVRSDTAVVHLQWANHEVGTIQPVAEVAARVA
                     ELDDVLFHVDAAQAAGRTAISFKRSGADLLSVSGHKLGGPTGVGALLVRRGVRLDPLI
                     TGGDQERARRAGIENVVAIAGFAAALNAATATLAAETERLEALRSRIVDWARDAAGVG
                     VLGHPDERLAQLVCLEIDGIEPQPILIGLDRVGIAVHSGNSCSSEALEPSPVLAAMGA
                     DAQHSLRISMGHLTTDEDIDRLLAELPRVLEQLWALRR"
     gene            complement(922..1380)
                     /locus_tag="PLP95_05845"
     CDS             complement(922..1380)
                     /locus_tag="PLP95_05845"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HPB45358.1"
                     /translation="MGIRVMIATDGTDESVEAAQRALDYIPVDAEVYLVSIIEGKYDP
                     ALDAGGFAGPLMDEDEAEERFQEAKEEGREAIERTERMLGARSVVERIVPSSASVPDA
                     IASVAREDDVDLIVVGSTQTGWFERHLLGGTDERLLRKAPCPVLVMTGDD"
     gene            complement(1495..1968)
                     /locus_tag="PLP95_05850"
     CDS             complement(1495..1968)
                     /locus_tag="PLP95_05850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB45359.1"
                     /translation="MSGRARFRTHPGSVLMWGVAHGALWALLALVLGFVEFVDVWKAV
                     AFGAVIGIGHSVLQWSTTSLSVEDGTLVSRNFTWVRRVPISEIELVQRARHRRWSGVV
                     VRLRDGDEIVLAAPVSSVMNPNPEFDEELRRLCASIVLGESEHDAEHDPDRRWDD"
     gene            2008..2508
                     /locus_tag="PLP95_05855"
     CDS             2008..2508
                     /locus_tag="PLP95_05855"
                     /EC_number="5.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate mutase family protein"
                     /protein_id="HPB45360.1"
                     /translation="MTIFLVRHGSAGHRNDSDPNDLDRHLDSTGLVQAERLAAVFSGA
                     EQLTGLGDLGVPAVERVWSSPAARCVETIEPTASRLGVSLSTSPHLLEGADIDGAWEL
                     IESLARESGDSVMCSHGDMIPELIRRARLRGMLVPTKSGCSKGSVWALSWDGDRFISG
                     SYATTR"
     gene            complement(2570..3793)
                     /locus_tag="PLP95_05860"
     CDS             complement(2570..3793)
                     /locus_tag="PLP95_05860"
                     /inference="COORDINATES: protein motif:HMM:NF027964.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase NADP-binding
                     domain-containing protein"
                     /protein_id="HPB45361.1"
                     /translation="MKILVIGSGGVGSAFATIVSRRGFFETLVLADIDLARAQRAATR
                     TGDARVSAMAVDASDSAAVAAAIANAGATLVLNAADPRFVMPIFEGCFAAGVTYIDMA
                     MSLSSPHPGDPHAVCGVKLGDEQFARHDAWAERGLLALCGMGVEPGLADVFARYASDH
                     LFSSIDEIGVRDGANLEVAGYDFAPTFSIWTTIEECLNPPVIWERERGWYTTEPFSEP
                     ETFVFPEGIGPVECVNVEHEEVLLVPRWIDCNRVTFKYGLGDEFIDVLKTLHKLGLDR
                     VDPVEVRGQRVSPRDVVAAVLPNPADLTDEISGKTCAGTWVSGTGTDGEHKEVYLYHV
                     VDNAESLARDGAQAVVWQTALFPAVAAELIAEGVWHGAGVLGPEAFDAVPFLDMVSGE
                     HRSPWGIEDRTATAS"
     gene            complement(3887..>4328)
                     /locus_tag="PLP95_05865"
     CDS             complement(3887..>4328)
                     /locus_tag="PLP95_05865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB45362.1"
                     /translation="GEATSSAGDGSAGDSPDGDALAKAATTLADAVKCFEGELDAHRD
                     NAQFLTLRSFTLGLLATIDNELAAATLPTALASADEALVIDPQDPTALLLRATWNMWL
                     GDYDASLADLDALGDREISPLVSPYVGAAEIRAEVTRQASASGG"
BASE COUNT          737 a         1538 c         1385 g          668 t
ORIGIN      
        1 ccgctacggc cgggtgtcgc ccgacgagct gcttgacgcg gtgcgcagcg acaccgccgt
       61 ggtgcacctg cagtgggcga accacgaagt cggcacgatc cagccggtcg ccgaggttgc
      121 agcacgcgtc gccgagctcg acgacgtgtt gtttcacgtc gacgccgccc aggccgccgg
      181 gcgcaccgcg atctcgttca agcgcagcgg tgccgacctg ctgtcggtgt cgggccacaa
      241 actcggagga cccaccggtg tgggagcgct gctcgtgcgg cgcggcgtac ggctcgaccc
      301 gctcatcacc gggggcgacc aggaacgagc gcggcgcgcc ggaatcgaaa acgtcgtagc
      361 catcgccggg ttcgccgccg cgctcaacgc tgcgacggcg acactcgcag cggagaccga
      421 gcggctcgaa gcgctgcgat cccgcatcgt cgactgggcc cgcgatgccg cgggggtcgg
      481 cgtgctcggc caccccgacg aacgcttggc gcaactcgtg tgtctcgaga tcgacggaat
      541 cgaacctcag ccgatcctga tcgggctcga tcgtgtcgga atcgccgtgc actcgggcaa
      601 ctcgtgcagt tccgaagcac tcgagccgtc cccggtgctc gcggcgatgg gagccgacgc
      661 ccagcactcg cttcggattt cgatgggtca cctcacgacc gacgaggata tcgatcggct
      721 gctcgccgaa cttccacggg tactcgagca gctctgggca ctgcgccgct gagccactcc
      781 gctgcaccga acactgcgcc gaaacagttc tgggcgcgct agcgagtcgg atgtgacccg
      841 cgagcgcgcc cagaactcgt ccaggcccgg cctcctgccg gaaaagccgc ccggcccgcc
      901 caagcaggac ggcggaaccg atcagtcgtc gccggtcatc accagcaccg ggcacggggc
      961 cttgcgcaac agtcgctcat cggtgccgcc gagcaggtga cgttcgaacc atccggtctg
     1021 ggtggagccg acgacgatga ggtcgacgtc gtcctctcga gcgaccgagg cgatcgcgtc
     1081 ggggaccgaa gccgacgatg gcacgatccg ctccacgaca cttcgtgcgc ccagcattcg
     1141 ttcggtgcgc tcgatggcct cgcgtccctc ttccttcgcc tcctgaaatc gttcctcggc
     1201 ctcgtcctcg tccatcagcg gaccggcgaa accgccagcg tcgagcgccg ggtcgtattt
     1261 cccttcgatg atggacacga gatacacctc ggcatcgacc gggatgtagt ccaacgcccg
     1321 ctgggcggcc tcgaccgatt cgtcggtacc gtcggttgca atcatcacac gaatacccac
     1381 agccgctcct cacgtcgtgc cttgttacga acgccaacct cgcgtgtacc tcgtggcgtc
     1441 agtgcaacgg tagcgcccac aaccgggggg cgtggcgtag gcggagcgcc ggactcagtc
     1501 gtcccaacgg cggtcggggt cgtgttcggc gtcgtgttcg gattcgccga gcacgatgct
     1561 ggcgcacagc cgccgcaact cctcgtcgaa ctcgggattg gggttcatta ccgaactcac
     1621 cggcgcggcc aacacgattt catcgccgtc gcgcagccgc accaccaccc ccgaccatcg
     1681 ccgatgacgc gcccgctgga cgagttcgat ctcggaaatc ggcacgcgac gaacccacgt
     1741 gaaattccga ctcacgaggg ttccgtcctc gaccgacaag ctggtggtcg accattgcaa
     1801 caccgagtgg ccgattccga tgaccgcgcc gaacgccacg gccttccaga catcgacgaa
     1861 ctcgacgaac cccaagacga gtgccagcag cgcccacaac gccccgtggg ccacacccca
     1921 catcaacacg ctgcccgggt gggttcgaaa tcgcgcacgg ccgctcacgc ttccacatta
     1981 cgttcgcggg ttgcctagtc tgcgtcggtg acgattttcc tcgttcgcca cggctcggcg
     2041 ggccaccgca atgattccga tcccaacgac ctcgaccgcc atctcgactc gacggggctg
     2101 gttcaggctg agcgattggc cgcagtcttt tccggtgcgg agcaactgac cggactcggc
     2161 gacctcgggg tcccggccgt cgagcgggtg tggtcctctc cagcggcacg ctgcgtggaa
     2221 accatcgaac cgaccgcgag ccgcctcggt gtgtcgttgt ccacctcgcc ccacctgttg
     2281 gaaggcgccg acatcgacgg agcgtgggaa ctgatcgaat cgctcgcccg cgagtcgggc
     2341 gactcggtga tgtgttccca cggcgacatg atccccgaac tcatccgacg cgcccggctg
     2401 cgcgggatgc tggttccgac gaagtcggga tgttcgaaag gctcggtgtg ggcgctgtcg
     2461 tgggacgggg atcgcttcat cagcggctcg tacgcaacga cccgctgacc accgaccacc
     2521 gaccgccggc cgcgcgacaa cgctgcccac ctggaccggg acgggctgct cagctcgccg
     2581 tagcggtgcg atcctcaatc ccccacggcg agcgatgctc gccggacacc atgtccagaa
     2641 acggcacggc atcgaaggcc tccggaccga gcaccccggc accgtgccac actccctcgg
     2701 cgatgagttc agcggcaacg gccgggaaca gcgccgtctg ccacacgacc gcctgtgcgc
     2761 cgtcgcgcgc cagcgactcg gcgttgtcga cgacgtggta caggtacacc tctttgtgct
     2821 cgccgtcggt gccggttccg ctgacccagg tgccggcaca tgtcttaccc gagatctcat
     2881 cggtgaggtc ggccggattc ggcagcactg cggccacgac gtcgcgcggc gacacccgct
     2941 ggccgcgtac ctcgaccgga tcgacccgat ccaacccgag cttgtgaagg gtcttgagca
     3001 cgtcgatgaa ctcgtcgccg agcccgtact tgaacgtcac gcggttgcag tcgatccaac
     3061 ggggcaccaa cagcacctcc tcgtgttcaa cgttgacgca ttcgaccgga ccgatgccct
     3121 cgggaaacac gaaggtctcc ggctcgctga acggctcggt ggtgtaccac ccgcgctcgc
     3181 gctcccagat caccggcggg ttgaggcact cctcgatcgt ggtccagatg gaaaatgtcg
     3241 gcgcgaagtc gtagccggcg acctcgaggt tggccccgtc gcgcacgccg atctcatcga
     3301 tggagctgaa gaggtgatcc gacgcgtacc gggcgaacac atcggcgaga cccggctcca
     3361 cacccatccc gcacaaggcg agcagcccgc gttctgccca ggcatcgtgg cgcgcgaact
     3421 gttcgtcacc cagcttcacc ccgcacacgg cgtgggggtc gcccggatgg gggctcgaca
     3481 gcgacatcgc catgtcgatg taggtcacac ccgccgcaaa gcagccctcg aagatcggca
     3541 tgacgaagcg aggatccgcg gcgttcagga ccaacgtggc ccccgcgttg gcaatggccg
     3601 cggcgaccgc cgccgagtcg gacgcgtcga ccgccatggc actcaccctg gcatcgcccg
     3661 tgcgggtcgc agcgcgttgc gctcgcgcaa gatcgatgtc ggcgaggacc aacgtctcga
     3721 agaacccccg ccgggacacg atcgttgcga acgcggaacc gacaccgccg gaaccgatca
     3781 cgaggatttt catggcgcga ctctacggga gcgtcgcact gaagcgtcgc gaaaagctcg
     3841 gcgcgtcgaa tgatctccgc gccgcggccc accccgaggc gcgcgatcaa ccccccgacg
     3901 cggatgcctg ccgggtcacc tcggcgcgga tctcagcggc gccgacgtag ggcgacacca
     3961 gcggggagat ctcgcgatcc ccgagcgcat cgaggtcggc caacgacgcg tcgtaatcgc
     4021 ccagccacat gttccaggtt gcccgcaaca gcagcgcggt cgggtcctgt gggtcgatca
     4081 cgagcgcctc gtcggcgctg gcgagggccg tgggcagggt cgccgcggcg agttcgttgt
     4141 cgatggtggc caagagcccc agggtgaagg accgcagggt caggaactgc gcgttgtcgc
     4201 ggtgagcgtc gagttcgccc tcgaagcact tcaccgcgtc ggccaacgtc gtcgcagcct
     4261 tcgccagcgc gtcgccatcg ggtgagtcac cggccgaacc gtctccggcc gacgaggtcg
     4321 cctcgccg
//
LOCUS       DAOOMA010000171         4299 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_24688_length_4299_cov_3.185674, whole genome shotgun sequence.
ACCESSION   DAOOMA010000171 DAOOMA010000000
VERSION     DAOOMA010000171.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 4299)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4299)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4299
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24688_length_4299_cov_3.185674"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            135..899
                     /locus_tag="PLP95_05870"
     CDS             135..899
                     /locus_tag="PLP95_05870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB45363.1"
                     /translation="MTDTDHTPNATTSPRSAHRRRRLAAVLTAAICAASVPAATPASA
                     QAVPVSVVVRTSPERPVPGDDVDVLVTVEGCPVGPITAEVYLETADGAKRSAVRIARA
                     AVDTSVTMRSTGAVRLPDAFEGWYGVRVLCGTFRPPRRSMTATLFHVRSSEAMTLQAP
                     EVQSLSDAFTMSGQRCPGGTVEWMVNSGFGGTTPFDPDGTVLVDDSGTWTVNAPWPEA
                     ITLGPMRASARCGYGLDTMTPRYLYYQPRWDLTAAP"
     gene            complement(925..2883)
                     /locus_tag="PLP95_05875"
     CDS             complement(925..2883)
                     /locus_tag="PLP95_05875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB45364.1"
                     /translation="MGESILDDVLGADNADSTAGVDGDRRLRIDRRRLLQGAAVASAG
                     GAVATLFQGRADAQSANKLEGYANLTSVDPGQTIRFFVTNGLAAGVRSTAAFAIKRVD
                     HPNDVQVHTASVSVEGQVTPANAWAVGCNWNETTSFTVPTSWPTGLYNAYFSEGSTRS
                     CIPFVVKPAVLGAASKILCQVPFTTLQAYNGWGGKCLYEFRSSNKVRSYKVSFDRPHD
                     NPEYGDLYQWVRPFIRFARRRGIEIDYVSTVDLHDKASLLDSYQMFVTVGHDEYWSRE
                     MRNNLDRFVRNGGNAAVFSGNTMYWQIRFEPNAAGAANRVITCYKDWNADPIADPARK
                     TVEWEEFGPSYKRALGTFSQHSSIGLGWERGGVWSGTGPRPANPFVIERPEHWAFAGT
                     GLAKGAQLAGELVGYETDALKWSRVNGVATPTGTDGAPSNFKILGIANLDSWTAVGQK
                     GGEAVMGTFRRNGTVFNVGTVDWAKGLTSPTAEVEKITMNVIEALSRVKTPRERRLQE
                     LYQYHAVNADGGWRFVFAPSPFFTQKNTGWIYDGPAFWVYTSPPADVEGVVEIKQYHA
                     VNPDGGWNIRYQLPEVATPEGYVYDGPAFFAHGTAGTGRYPIYLYTAPSAGSFDRVVY
                     SPTPNLGRGWNLEGVAFYAPTPPSRLSD"
     gene            complement(2991..3698)
                     /gene="alr"
                     /locus_tag="PLP95_05880"
     CDS             complement(2991..3698)
                     /gene="alr"
                     /locus_tag="PLP95_05880"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HPB45365.1"
                     /translation="MRLAGLATHLATADEVAPSAGAALCDAQLAAFSEVDAQVAAMVE
                     GRSGSATDRSTNGDRGYVRHVANSPAALTRPQAHLDMVRCGIAVYGIAPSPAQPIPVE
                     LTPAARLVSAVTWTKRVGADEAVSYGARYRTPRETTIATVPVGYADGVARSLTGAGAE
                     VLINGRRAPIVGTVTMDQLMIDVGDEPVAIGDEVVLIGRAGADEITANDLAETIGTIG
                     YEVVTRLSARLPRVVVP"
     gene            complement(3710..4177)
                     /locus_tag="PLP95_05885"
                     /pseudo
     CDS             complement(3710..4177)
                     /locus_tag="PLP95_05885"
                     /inference="COORDINATES: protein motif:HMM:NF013345.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
BASE COUNT          696 a         1535 c         1397 g          671 t
ORIGIN      
        1 cggtcacaca ctactggcac cgcgacccgc catccctcga caccccaccc gttcacaccc
       61 ctgcggtggc gacgttggtt cgctgcgaga aacgccgtac gctcatgcat cggatcccgc
      121 ctccatggaa tgacatgacc gacaccgacc acacgccgaa cgccacgact tccccgcgct
      181 ccgcgcaccg ccggcgccgc ctcgccgcgg tgctcaccgc agcgatctgc gcggcgtcgg
      241 ttccggccgc gaccccggcg tcggcgcagg cggtgccggt ttcggtggtg gttcggacct
      301 cccccgagcg cccggttccc ggcgacgacg tggacgtcct cgtcaccgtc gagggttgcc
      361 cggtgggccc catcaccgcc gaggtgtacc tcgagaccgc cgacggagcg aaacgttcag
      421 ccgtgcggat cgcgcgagcg gcggtggaca cctcggtgac gatgcgttcg acgggcgccg
      481 tgcggctccc cgacgcgttc gaggggtggt acggggtgcg ggtgttgtgc ggcacgtttc
      541 gtccgccgcg ccgctcgatg accgccacgt tgttccacgt ccgctccagc gaggcgatga
      601 cgctgcaagc ccccgaggtt caatcgctgt cggacgcgtt cacgatgtcg gggcaacggt
      661 gccccggcgg cacggtcgag tggatggtga acagcggatt cggcggtacc accccgttcg
      721 accccgacgg cacggtcctg gtcgacgaca gcggaacgtg gaccgtgaac gcgccctggc
      781 ccgaggccat caccctcggg ccgatgcggg catcggcgcg atgcggctac ggcctcgaca
      841 cgatgacccc tcgctacctc tactaccagc cccgctggga cctgacggcg gccccctgag
      901 cgcagcgctc aggagcacca gcggtcagtc ggacagtcgc gacggcgggg tcggggcgta
      961 gaaggcgaca ccctcgaggt tccacccgcg accgagattc ggggtgggcg agtagacgac
     1021 ccgatcgaac gagcccgccg acggcgccgt gtacagatag atcgggtagc ggccggtgcc
     1081 ggcggtgccg tgcgcgaaga acgctggccc gtcgtacacg tagccctctg gagtggcgac
     1141 ctcggggagc tgatagcgga tgttccaacc gccatcgggg ttcaccgcat ggtactgctt
     1201 gatttccacg acgccttcga cgtctgccgg tggcgaggtg tagacccaaa acgccgggcc
     1261 gtcgtagatc cagccggtgt tcttctgggt gaagaacggc gagggggcga acacgaaacg
     1321 ccatccccca tccgcattga ccgcgtggta ttgatacagc tcctgaaggc ggcgctcgcg
     1381 cggggtcttc accctcgaga gcgcctcgat cacattcatc gtgatctttt cgacctcggc
     1441 ggttggcgag gtcagaccct tcgcccagtc gaccgttccg acgttgaaca ccgttccgtt
     1501 tcgacggaac gtccccatga cggcctcgcc gcctttttgc cccaccgccg tccaggaatc
     1561 gaggttggcg atgccgagaa tcttgaaatt cgacggggca ccatcggtgc cggtcggcgt
     1621 tgcaacgccg ttgacccgcg accacttgag ggcgtcggtc tcatagccga ccagctcgcc
     1681 ggcgagctgc gcacccttcg ccagtccggt gccggcgaag gcccagtgtt ccggtcgctc
     1741 gatgacgaac gggttggccg gacgcggtcc ggtaccggac cagaccccgc cacgctccca
     1801 gccgagcccg atcgaggagt gctgcgagaa cgtccccaac gcgcgcttgt agctcggccc
     1861 gaactcctcc cattcgacgg tctttcgcgc cgggtcggcg atcgggtcgg cgttccagtc
     1921 cttgtagcag gtgatgaccc gattcgccgc gcccgcggcg ttcggctcga agcggatctg
     1981 ccagtacatg gtgttgccgc tgaacaccgc cgcattgccg ccgttgcgca cgaaccggtc
     2041 gaggttgttg cgcatctccc gcgaccagta ctcatcgtgg ccgacggtga cgaacatctg
     2101 atagctgtcg agcagcgacg ccttgtcgtg aaggtcgacc gtcgacacgt aatcgatctc
     2161 gatgcctcgc cgacgagcga accggatgaa cggacgaacc cactgataaa ggtcgccgta
     2221 ctcggggttg tcgtggggac ggtcgaagct gaccttgtac gagcgcacct tgttggagga
     2281 ccggaactcg tacaggcact tgccgcccca gccgttgtag gcctgaagcg tcgtgaaggg
     2341 aacctggcag agaatcttgc tggcggcgcc cagcaccgcg ggcttcacga cgaaaggaat
     2401 gcacgagcgg gtggaaccct cgctgaagta cgcgttgtac agccccgtcg gccacgaggt
     2461 cgggacggtg aagctcgtgg tctcattcca gttgcatccg accgcccacg cgttcgccgg
     2521 agtcacctga ccttcgacgc tgacggacgc cgtgtggacc tgaacgtcgt tcgggtgatc
     2581 gacgcgcttg atggcgaacg ccgccgtcga acggactccg gcggccagac cgttggttac
     2641 gaaaaagcga atcgtctggc ctgggtcgac cgaggtgagg ttggcgtagc cctcaagctt
     2701 gttggccgac tgggcatcgg cccgaccctg aaacagcgtc gcgacggcgc cgccggccga
     2761 ggccaccgcc gcgccctgga gcagccgacg acggtcgatg cgcaggcgcc gatccccatc
     2821 tacgccagca gtgctgtcgg cgttgtcggc gccgagaacg tcgtcgagaa tcgattcacc
     2881 catctggtcg acgatacgga tcgacaccaa attccacaac cgccctggga agttttcccg
     2941 agtgacccgg gccgaccggc ccgagatcgt cggcaccttt gggcgatgtc ctacggcacc
     3001 acgaccctcg gcaaccgggc cgacaatcgg gtgaccacct catagccgat cgtcccgatc
     3061 gtctcggcca gatcgttggc ggtgatctca tcggcgccag cgcggccgat cagcacgacc
     3121 tcgtcgccga tcgccaccgg ttcgtcgccg acgtcgatca tcagttgatc catcgtcacc
     3181 gttcccacga tcggggcgcg acgaccgttg atgagcacct ccgcgccggc tccggtcagc
     3241 gacctggcga cgccatcggc atagcccacc gggaccgtcg cgatcgtcgt ctcacgaggt
     3301 gtgcgatacc gagccccgta cgacacggcc tcatcggcac ccacccgttt ggtccatgtc
     3361 accgccgaga cgagtcgagc ggccggggtc aactcgaccg gaatcggctg cgccggcgac
     3421 ggggcgatgc cgtagaccgc gatgccacat cgcaccatgt cgaggtgcgc ctgcgggcgg
     3481 gtcagtgccg ccggcgagtt cgccacgtga cgcacatagc cgcggtcgcc gttcgtcgat
     3541 cggtccgtcg ccgacccgga gcggccctcg accatcgccg cgacctgcgc gtcgacctcg
     3601 gaaaaggcgg ccagttgggc gtcgcacagc gccgcaccag cgctcggcgc gacctcatcc
     3661 gcggtggcga ggtgggttgc gagcccggcg aggcgcaccc cggcgtgtcg aggatcaggc
     3721 cggcgagttc gagcgcatcg ccgacctcgc agccgacgcg ccgcatgccg gtgtcgacct
     3781 tcagatgaag ccccacctcc acctcgcctc gtgcgccgcc gacggacccc gccccagcgg
     3841 ccgccgcggc cgcctcgatg tcgtcgcgcg agtacacgaa cgactcgagc cccaactccg
     3901 ccaccgtcgc ccagtcgtcg cgctggggcg ccgacaacac caggatcggc gcgtcgattc
     3961 cggcctcgcg cagctccacc gcctcctcca ccaaggccac ggccaacacg ctcgctccgc
     4021 cggcgagcgc ctcgcgtgac gccagcacgg ccccgtgtcc gtacgcgttg gctttcacca
     4081 ccgcgcacac cgcggccgga gcacacaact cgacgagcgc ctcgacgttc gaacgaagcg
     4141 ccgacgccga cacctcgatg cgactcggtc gcgtcatcgt cgcgtccgcc gcgggcccgt
     4201 cgcacgttgt tcggcgacgg cctcggacaa cacctcggaa atcaccggtg ggagatcgcc
     4261 ggcgaccgtg cccaccagcg gcaggcgccg ggctgcgag
//
LOCUS       DAOOMA010000172         4291 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_24745_length_4291_cov_3.902502, whole genome shotgun sequence.
ACCESSION   DAOOMA010000172 DAOOMA010000000
VERSION     DAOOMA010000172.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 4291)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4291)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4291
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24745_length_4291_cov_3.902502"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1627
                     /locus_tag="PLP95_05890"
     CDS             <1..1627
                     /locus_tag="PLP95_05890"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     ATP-binding protein/permease"
                     /protein_id="HPB45366.1"
                     /translation="LTIPIGLVFAIPALRTRGVNLAVVTLALGFLVSEVVFANPNYLG
                     EPLDGGTRIGTITLFGMEVDALDHPHAWAVVCLVFLVGLSLMVANLRRSRTGHRLLAV
                     RTNERAAASLGISVFGVKLYAFAMSAGLAAVAGILFAFRSQVITYHEFNLLASIGSLG
                     NAVIGGLGYVLGAPMAAPMVNGGLGTRVMEDWIGLANQWDQIVGAVLVFVILIFNQNG
                     VADVATRRRQIWEKLHLVRPRARRTELGAVNEEMVAVDGSVLKVSGLSVTFGTVQALH
                     DVSLEVRPGEVVGLIGPNGAGKTTFIDAVTGFVDSSAGQIDLGDHRLDKLSATRRARL
                     GLRRSFQSLELFEDVSVEQNILAGADIKEPLHSWFTDLFWPTRHELPPAAVAAVRSFE
                     LEADLDSSPEELSYSRRRVVGIARTVASGPSVTLLDEPAAGLDEHESMEFAGLIRALA
                     DQRNMGVLLVEHDMNLVMSICDRLVVLEFGQVIASGTPEEIRNNAAVRAAYLGESVDV
                     ESVGSGAESGGAVGAESDGAVASADTPIEEVGS"
     gene            1624..2313
                     /locus_tag="PLP95_05895"
     CDS             1624..2313
                     /locus_tag="PLP95_05895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPB45367.1"
                     /translation="MNGSLIEAVGVSSGYGKVSVLRGVDLSVAAGEVVALLGGNGAGK
                     TTTLLTLAGELTPTEGEVRFLGSSCTDPMHVRCRNGLSYLTEDRSVIMEMTVKDNLRL
                     ANVSPEVATEYFPALERILGRRAGLCSGGEKQMLALARCLGRNPKVLLADELSLGLAP
                     LVVGGLLETLRRVADEQGIGVLLVEQHIRQALEIADRAYVMERGRIVLSGTAAEVSEQ
                     IGKVQSAYLAG"
     gene            complement(2427..3032)
                     /locus_tag="PLP95_05900"
     CDS             complement(2427..3032)
                     /locus_tag="PLP95_05900"
                     /inference="COORDINATES: protein motif:HMM:NF012493.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C-terminal helicase domain-containing protein"
                     /protein_id="HPB45368.1"
                     /translation="MNKNRFEILKSLSLLRQLSLAPGLIDDEYDYIGSAKIDRLVEDL
                     GQILAEGHRALVFSQFTSFLSRVRDRLDDEGITYAYLDGRTRRRDEAISSFKNGDSPV
                     FLISLKAGGFGLNLTEADYCFVLDPWWNPATEAQAIDRTHRIGQTNPVIVYRYVSAGT
                     IEEKVMELKERKAALFDSVMSDDGLSGALTEADIRNLFSDT"
     gene            complement(3075..>4291)
                     /locus_tag="PLP95_05905"
     CDS             complement(3075..>4291)
                     /locus_tag="PLP95_05905"
                     /inference="COORDINATES: protein motif:HMM:NF012403.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140658 - ATP-dependent chromatin
                     remodeler activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SNF2-related protein"
                     /protein_id="HPB45369.1"
                     /translation="AGSGDSTIISPFNDWFDLSIEVTIDGHEVPFAQLFTALALGEAT
                     LILDDGSWVSTDRPEFEQLRALIEEARGLDGLADDASSVRINRFQTDWWDEITRLGVV
                     ASQSDRWQRSVAQLGELTAPTPVPTPLSLDAKLRDYQHVGLDWLAFLQRSHLGGVLAD
                     DMGLGKTVQVLALFLHLLEEHEFASAEFASGGIEADAGGSPGSKTPERPRFLVVAPTS
                     VVRNWQHEAERFAPDLVVRCVTETEARRLAPLSAVVDGADVVVTSYTLFRLEFDDYAE
                     HRWTTLVLDEAQMVKNHRSKAYLCVRRLEADTKIAITGTPLENSLMDLWSLLSITAPG
                     LYPDPKRFAEVYQRPIERGFAPERLATLRRRIAPLMRRRTKDEVLAELPPKTELEVPV
                     DLSPKHKKLYDT"
BASE COUNT          703 a         1307 c         1480 g          801 t
ORIGIN      
        1 gttgaccatc ccgatcgggc tcgtgttcgc catccctgca ttgcgaaccc gcggcgtcaa
       61 cctggcggtc gtcacgttgg cgctcgggtt cctcgtgtcc gaagtggtgt tcgccaaccc
      121 gaactacctc ggcgaacccc tcgacggtgg aacgcggatc ggcaccatca ccttgttcgg
      181 catggaggtc gacgccctcg atcatccaca cgcctgggcc gtggtgtgtc tggtgttcct
      241 cgtcgggctg tcgctgatgg tggcgaacct gcgccggtcc agaaccgggc accgcctgtt
      301 ggcggttcgc acgaacgaac gggccgccgc gtcgttggga atctcggtgt tcggggtcaa
      361 gctctatgca ttcgccatgt ccgcaggcct cgccgcggtg gccggcatcc tgttcgcgtt
      421 tcgcagccag gtcatcacct atcacgagtt caacctgttg gcgtccatcg gcagcctcgg
      481 caatgcggtg atcggcggcc tcggctatgt gctgggcgca cccatggcgg cgccgatggt
      541 caacggcggt ctgggaacgc gagtgatgga ggactggatc ggcctcgcca accagtggga
      601 tcagatcgtc ggagccgtcc tggtgttcgt catcctcatc ttcaaccaga acggtgtcgc
      661 cgacgtggca acccggcgcc ggcagatctg ggagaagtta catctcgtca ggccgagggc
      721 acggcgcacc gagttgggtg cggtgaacga ggagatggtc gcggtcgatg ggtcggtgtt
      781 gaaggtgtcg ggcctttcgg tgaccttcgg gacggtgcag gcgttgcacg acgtgtcgct
      841 cgaggtccgc cccggcgagg tcgtgggact gatcgggccg aacggtgccg gaaagacgac
      901 gttcatcgac gccgtcacgg gtttcgtcga ttcctcggcc ggccagatcg acctgggcga
      961 ccatcgcctc gacaagctga gcgccaccag gcgtgcgcgt ttggggcttc gccggtcgtt
     1021 tcaatcgctc gagttgttcg aagacgtgag cgtcgaacag aacatcctcg ccggtgccga
     1081 catcaaagag ccgctgcact cgtggttcac ggacctgttc tggccgaccc gccacgagct
     1141 gccgcccgcg gcggtcgctg cggttcgcag tttcgaactc gaggccgatc tcgattcgag
     1201 tcccgaggag ctttcctaca gtcgccggcg ggtcgtgggc atcgcccgca cggtggcctc
     1261 gggtccgtcg gtcacgctgc tcgatgagcc cgccgccggc ctcgacgaac acgaaagtat
     1321 ggaattcgcg gggctcatca gggcactggc ggatcaacga aacatgggcg tgctgctggt
     1381 ggaacacgac atgaatctgg tcatgtcgat ctgtgatcgc ctggtggtgc tggagttcgg
     1441 acaggtgatc gcgagtggca cgcccgagga gatccgcaac aatgctgcgg ttcgagcggc
     1501 gtacctcggc gagagcgttg acgtggaaag cgtcgggtcc ggcgccgagt ccgggggtgc
     1561 tgtgggcgcc gagtccgacg gtgctgtcgc aagtgccgac acgccgatcg aggaggttgg
     1621 atcatgaacg gaagcctcat cgaagcggtt ggcgtttcgt cgggatacgg caaggtctcg
     1681 gtgttgcgcg gcgtcgattt gagcgttgcc gccggcgagg tcgtggcgtt gctcggcggc
     1741 aacggtgccg gaaagaccac cacgttgttg accctcgccg gcgagttgac ccccaccgag
     1801 ggcgaagtgc gtttcttggg ctcgagttgc accgacccga tgcacgtccg ctgccgcaac
     1861 gggctcagct atctcaccga ggaccgttcg gtgatcatgg agatgacggt gaaggacaac
     1921 ctgcgactcg ccaacgtctc gccggaggtg gccacggagt acttcccggc gttggaacgg
     1981 atcctcggcc gccgtgcggg gctgtgttcc ggtggcgaga agcagatgct ggctctcgcg
     2041 cgttgcctcg gccgtaaccc gaaggtgctc ctcgcggacg agttgtccct cggcctcgca
     2101 cccctcgtgg tggggggact gctggagacg ctgcgtcgcg tggccgacga gcaggggatc
     2161 ggggtgttgc ttgtcgaaca acacatccgc caagcgttgg aaatcgccga tcgtgcctat
     2221 gtgatggagc gcggacgcat cgtgttgagc ggtaccgcag ccgaggtcag cgagcagatc
     2281 ggcaaggtcc agtcggcgta cctcgccggc tgaccagcgc ccaggccacc cgagttgccc
     2341 gttctccgat aaccagtggt cgtgatcacg accactggtt atcggagaac aggtagggcg
     2401 gctccgggga acaggtttgg ggcggctcaa gtgtcggaga acaggttgcg gatgtccgcc
     2461 tccgtcaacg cgcccgacag cccgtcgtcg gacatgacac tgtcgaacag cgcggccttg
     2521 cgctccttca actccatgac cttctcctcg atggtgcccg ccgagacgta gcggtagacg
     2581 atcaccgggt tggtctggcc gatgcggtgg gtccggtcga tggcctgcgc ctcggtcgcc
     2641 gggttccacc aggggtcgag cacgaagcag tagtcggcct cggtgaggtt caacccgaac
     2701 ccgcccgcct tgaggctgat gagaaagacc ggggaatcac cgttcttgaa cgaggagatc
     2761 gcctcgtctc gacgtctggt cctgccgtcg aggtaggcgt aggtgatgcc ctcgtcgtcg
     2821 aggcgatccc ggaccctcga caggaagctc gtgaactggc tgaagaccag cgcccggtgc
     2881 ccctccgcga gaatctgtcc gaggtcctcg acgagtcggt cgatcttggc cgagccgatg
     2941 tagtcgtact cgtcgtcgat gaggcccggc gcgaggctca actggcgcag caacgacaac
     3001 gatttcagga tctcaaaccg gttcttgttc atgtcgccga cgagacccaa cacctttcgt
     3061 cgctgctgtt gcagttaggt gtcgtagagc ttcttgtgtt tcggactgag gtcgaccggt
     3121 acctcgagtt cggtcttcgg tgggagctcc gcgagtacct cgtccttggt gcgacgtcgc
     3181 atgagtgggg cgattcgccg ccgcagcgtc gccaaccgct cgggcgcgaa cccgcgctcg
     3241 atcgggcgtt ggtagacctc ggcgaatcgt ttcggatccg ggtagagccc cggagcggtg
     3301 atcgagagca atgaccacag gtccatcagg ctgttttcga gcggcgttcc ggtgatggcg
     3361 atcttggtat cggcctcgag tcgacgcaca cagaggtacg ccttggagcg gtggttcttg
     3421 accatctgcg cctcatcgag cacgagcgtg gtccagcgat gttcggcgta gtcgtcgaac
     3481 tcaagacgaa acagcgtgta ggaggtgacg acgacgtctg ccccatcgac gacggcgctc
     3541 agcggggcga ggcggcgggc ctcggtctcg gtcacgcagc gtacgacgag gtcgggagcg
     3601 aatcgctcgg cctcgtgttg ccagttgcga acgacactcg tcggggcgac gacgaggaaa
     3661 cgcggacgct caggggtctt cgagccgggg ctcccaccgg cgtcggcctc aatcccgccg
     3721 gacgcgaact cggcggaagc gaattcgtgc tcctcgagga ggtggagaaa cagcgccagg
     3781 acctgcacgg tcttgccgag gcccatgtcg tcggccaaca ccccgccgag gtgcgatcgc
     3841 tgcaggaaag ccagccagtc gaggccgacg tgttggtagt cgcgaagctt ggcgtcgagc
     3901 gacagcgggg tcgggaccgg tgtcggcgcg gtcagctcgc cgagttgggc aacgctgcgc
     3961 tgccatcggt cggactgcga tgcgacgact ccgagccggg tgatctcgtc ccaccagtcg
     4021 gtctgaaacc ggttgatacg caccgacgat gcgtcatcgg cgagcccgtc gaggcctcgg
     4081 gcttcctcga tcagggcacg cagctgttcg aactccgggc gatcggtgct gacccatgag
     4141 ccgtcgtcga gaatcagtgt cgcctctccc agtgccagcg ccgtgaacag ctgggcaaat
     4201 ggcacctcgt gaccgtcgat cgtgacctcg atggacaggt cgaaccagtc gttgaacggg
     4261 ctgatgatcg tcgagtcgcc ggagcccgcg g
//
LOCUS       DAOOMA010000173         4281 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_24826_length_4281_cov_4.412683, whole genome shotgun sequence.
ACCESSION   DAOOMA010000173 DAOOMA010000000
VERSION     DAOOMA010000173.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 4281)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4281)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4281
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24826_length_4281_cov_4.412683"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(68..1381)
                     /locus_tag="PLP95_05910"
     CDS             complement(68..1381)
                     /locus_tag="PLP95_05910"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HPB45370.1"
                     /translation="MTAAPVITRSRYRGRSWDPLIRPLAVAALALCLLFNSRAVLAID
                     GVLIPNTVPLLLGLGAIAMAIIVPRPETVIIPIAPTLLMLLLIASVGWSADPDNAILW
                     LRSDMIVAVGVAALVLALPNRDVIAGLKLFVGVALAVTLLAVVVDPLARMHIDPTGEA
                     PDLTGWHGWFIHKNTMAGFMVVALPTVMAFYRTRWARWTGYAVIVVLMIGSDSTTGRS
                     ALVVVAAVRVWVAFNRRLSSRGSATFLVSTIALVALAIIAAVSSLATLADAAGKDLTF
                     TGRTQIWSAVLNAWAERPILGYGIKGLFAVPLSSMSLQLMREIGFKAGHAHSGPLDVG
                     LQLGLVGLIAVIGVLVGIYRASIRELRRSLSVPMFCLTVIVGITIMSVGESALLGPNI
                     AVFFILRAMLLKTARANDLAEVARLDELRRGPQVVDATVGPGPAR"
     gene            complement(1387..2316)
                     /locus_tag="PLP95_05915"
     CDS             complement(1387..2316)
                     /locus_tag="PLP95_05915"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HPB45371.1"
                     /translation="MSGAVEPRRVVVSICTYRRNEPLRRLLGALAAQANSIETMTVGV
                     SIIDDSPEAGARSVVSQFEGRFSGGIGYTSTASGNISTARNAAIATALDMDADWVMFI
                     DDDCLPHERWIEIMASTQQRTGADLVTGPVEDIAPPNAPAWLTQQPFLNLIAQYTDGE
                     EPPYGTTANVFISAAWLRAHPDVRFRSQLGELGGEDMVWFDTARSAGIVHRYSLHAPV
                     YEQVPLERCRFGYQLRNKLWFGNTMYVTSSHRGEAPNRLLLRGIKYLVNGLAMPLRRI
                     VRREDPHLRYSLATICIGIGLVGGRFGLRLKHH"
     gene            complement(2313..3290)
                     /locus_tag="PLP95_05920"
     CDS             complement(2313..3290)
                     /locus_tag="PLP95_05920"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HPB45372.1"
                     /translation="MTPRVTIFIPTYERAAYLRDAIDSALAQTMPDFELLIGDNSRTD
                     GTEEVVRTYDDPRIRYVRHPENLGQQGNWLWLIENASAPFVASLHDDDVWCPDFLADL
                     LPVIEADESVSMVFSDYDLIDGEGSVLTEETAELRERSHRDRLPAGRLDIDLAGALRL
                     IAVWNAPQPAYCAILRRTAVLDCEFPPEIDPVYDIWLSYQIARRSESFAYVPGVHTLY
                     RWHGGSSTTVGWAPQEDEIFSRIIVENHGLGPVIEEIERYWSFIRWGRGVRMMEDRSQ
                     CAESRRTLRAAAPRLDPARKVLATAASRSPHMWNGLSALRSRSRQIRSR"
     gene            complement(3405..>4281)
                     /locus_tag="PLP95_05925"
     CDS             complement(3405..>4281)
                     /locus_tag="PLP95_05925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HPB45373.1"
                     /translation="VQSAVVLAGLGYEVELCAEFQPDAINVVSSVDLRISDRTAHAFV
                     VAFRADWARPILGDLTLTMNETIATRPTEFALPHWTQTGLVRRDPDRPSRVENLVFKG
                     DLVNLDARFRSDSFGRELARRDIRFRCDVFDHATQTSGWNDYRDVDAVLAVRDIPPTE
                     TATKPASKLINSWAAGVVAILGNEPAYRELRRHDLDYFEVSTPQQALDALDRLNRNPR
                     FYDAVVGQGRIRAADYTNERTAERWMALLNGVVADAFERWLAENPLHRTARHAVRAVG
                     QRVENAVFRRRQATR"
BASE COUNT          852 a         1393 c         1325 g          711 t
ORIGIN      
        1 ctgcccgacg tagcggcgat gaacgagttc accatctggc aatcccaggc tcccaccatc
       61 tgggcgttca gcgtgctgga ccaggcccga cggtagcgtc gacgacctgc ggaccgcgcc
      121 tcaactcgtc aaggcgggct acctcggcga gatcgttggc cctcgcggtc ttgagcagca
      181 tcgcacgcag gatgaagaac accgcgatat tggggccgag caacgctgac tccccgaccg
      241 acatgatcgt gatcccgacg atcaccgtga ggcagaacat cggaaccgac aacgaccggc
      301 ggagctcacg gatcgacgcc cggtagatcc ccaccaacac gccgataacc gcgatcagtc
      361 cgaccagacc gagttgcagc cctacgtcga ggggaccgct gtgggcgtga ccggccttga
      421 aaccgatctc gcgcatgagt tggaggctca tcgaactcag cggtacagcg aaaagaccct
      481 tgatgccgta cccgaggatc gggcgctcgg cccacgcgtt caacacggcc gaccagatct
      541 gcgttcgccc ggtgaacgtc aggtctttgc cggcagcgtc ggcgagtgtg gccaacgaac
      601 tcaccgcggc gatgatggca agcgcgacga gcgcgatcgt ggacaccagg aaggtggccg
      661 agccacgact cgacagtcga cggttgaacg ccacccagac ccgcaccgct gccaccacga
      721 ccaacgccga cctgccggtg gttgagtccg atccgatcat cagcaccaca atcacggcat
      781 aacccgtcca ccgtgcccaa cgggtgcggt agaaggccat gaccgtcgga agcgcgacga
      841 ccatgaaccc ggccatggtg ttcttgtgga tgaaccaccc gtgccagccg gtgaggtccg
      901 gcgcctcgcc agtcggatcg atgtgcatcc gcgcgagcgg atccaccacg accgccaaca
      961 gggtcaccgc aagcgcaact cccacgaaca gcttcaatcc ggcgatcacg tcacggttcg
     1021 gcaacgccag caccaacgcc gccaccccaa ccgcgacaat catgtcggaa cgcagccaca
     1081 ggatcgcgtt gtccggatcg gccgaccatc cgaccgatgc gatcaacaag agcatcaaca
     1141 acgtcggagc gatcggaatg atgacggtct cgggtcgggg aacgatgatc gccatcgcga
     1201 tcgccccgag gccgagcaac aacggcaccg tgttgggaat caagacgccg tcgatcgcca
     1261 gaacggcgcg actgttgaac aggaggcaca atgcgagcgc agcgaccgcc aagggacgta
     1321 tcaacgggtc ccacgagcgt ccccgatatc tggatctggt gatgacgggc gcagcggtca
     1381 tcggcatcag tggtgtttca accgtaggcc gaaccgcccg ccgaccagcc cgatgccgat
     1441 gcagatggtt gccagggaat agcgaaggtg ggggtcctca cgtcggacga tgcgacgcaa
     1501 cggcatggca agaccgttca ccaggtactt gatgccgcgt aacagcaggc gattcggcgc
     1561 ctctccgcga tgcgacgacg tgacgtacat cgtgttgccg aaccacagct tgttgcgcag
     1621 ttggtatccg aaacgacacc gctcgagcgg gacctgttcg tacaccggtg catgcaggct
     1681 gtagcggtga acgatccccg ctgagcgcgc cgtgtcgaac cacaccatgt cctcgccacc
     1741 gagttctccg agttgcgagc gaaaccgcac gtcgggatgg gctcgcaacc acgctgccga
     1801 gatgaacacg ttggccgtcg tcccgtaagg cggctcctcg ccatcggtgt actgcgcgat
     1861 gaggttgaga aacggttgtt gcgtcagcca tgccggggcg ttcgggggag cgatatcttc
     1921 cacgggcccg gtgaccaggt cggcaccggt tcgctgctgt gtcgacgcca tgatctcgat
     1981 ccagcgttcg tggggaagac agtcatcgtc gatgaacatc acccagtcgg catccatgtc
     2041 gagcgcggtg gcgatcgctg cgttcctcgc cgtggagata ttgcccgaag ccgtcgaggt
     2101 gtaaccgatc cctccggaga atcgcccctc gaattgcgac acgacgcttc gagcgcccgc
     2161 ttcggggctg tcgtcgatga tcgacacacc gacggtcatg gtctcgatcg aattggcctg
     2221 tgcggcgagc gcaccgagga gccgtcgcag gggttcgtta cgccggtacg tacagatcga
     2281 caccacgact cgccgtggct cgacagcccc actcatcgtg accgaatctg tcgcgatcgg
     2341 gagcgcaatg cgctcaaacc gttccacatg tgcggggaac ggctcgcggc ggtcgccaac
     2401 actttccgag ccggatcaag ccgcggcgcc gctgcccgga gcgtgcgccg ggattcggca
     2461 cattgcgagc ggtcttccat catgcgcact ccccgccccc aacggatgaa cgaccagtac
     2521 cgctcgattt cctcgatcac cggaccgagc ccatggttct cgacgatgat tctggagaag
     2581 atctcgtcct cctgaggggc ccaaccgacg gtggtcgagg aaccgccgtg ccaccggtac
     2641 agcgtgtgaa ccccggggac gtaggcgaac gattcgctgc gtcgggcgat ttggtaggac
     2701 agccagatgt catacaccgg gtcgatttcc ggcgggaact cgcagtcgag cactgccgtc
     2761 cggcgcagga tcgcgcaata ggcgggctgc ggagcgttcc acaccgcaat cagacgaagt
     2821 gccccggcga ggtcgatgtc caggcggccg gcgggcaagc ggtctcggtg agagcgctcg
     2881 cggagctcgg cggtctcctc ggtcaacact gaaccctcac cgtcgatcag gtcataatcg
     2941 gaaaacacca tggaaacgga ctcatcggct tcgatgaccg gcaacagatc cgccaggaag
     3001 tcgggacacc agacatcatc gtcgtgcagc gacgcgacga acggcgccga ggcgttctcg
     3061 atcagccaca accagttgcc ctgctggccg aggttctccg ggtggcggac atagcgaatc
     3121 cgcgggtcgt cgtaggtccg aacgacctcc tcggtgccat cggttcgact gttgtcgccg
     3181 atcaacagct cgaagtccgg catcgtctgc gccaacgccg aatcgatcgc gtcgcgcagg
     3241 taggccgctc gttcataggt tggaatgaag atggtgaccc tcggggtcat ggttgctgcg
     3301 ctcctccggc tccggtcaat cgctctacca atgctgtcgg cgttcagcgc cgacaaataa
     3361 gtgctgtccg gctcatccgc accggccgtt cgacctagtg tcgttcaccg cgtcgcctga
     3421 cgccgtcgaa agaccgcgtt ctcgacgcgc tgacccaccg ctcgcacggc gtgccgcgcc
     3481 gtgcgatgca acggattttc agccaaccac cgctcgaacg catccgccac cacgccgttg
     3541 agcaacgcca tccaacgctc ggcagtgcgc tcgttggtgt agtccgcagc acgaatgcgg
     3601 ccttggccaa cgacagcgtc atagaacctc ggatttctgt tgagccgatc caacgcatcg
     3661 agcgcctgtt gtggtgtgga aacctcgaaa taatcgaggt catggcgtcg cagctcgcga
     3721 taggcaggct cgttgccgag gatcgccacg accccagccg cccaggagtt gatcagtttg
     3781 ctggccggct tggtagcggt ttcggtcggg gggatgtctc gcacggccag caccgcgtcg
     3841 acgtctcggt agtcattcca gccgctcgtt tgtgtggcat ggtcaaaaac gtcgcagcgg
     3901 aaccggatgt cgcgacgggc gagttcccgg ccgaacgagt cgcttcgaaa ccgggcgtcg
     3961 aggttcacga gatcgccttt gaacacgaga ttctcaactc gactcggccg atcgggatcg
     4021 cgcctcacca gtcccgtttg cgtccagtga ggtagagcga actcggtcgg ccgagtggcg
     4081 atggtctcgt tcatggtgag cgtcagatcg ccaagaatcg gccgcgccca atcggcgcgg
     4141 aacgcgacga cgaaagcgtg ggcggtgcga tccgagatcc gcaggtcgac actgctcacg
     4201 acattgatcg catcaggctg gaactcggcg cacaattcaa cttcgtagcc caagcccgcc
     4261 aagaccaccg cgctctgcac g
//
LOCUS       DAOOMA010000174         4268 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_24915_length_4268_cov_4.254925, whole genome shotgun sequence.
ACCESSION   DAOOMA010000174 DAOOMA010000000
VERSION     DAOOMA010000174.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 4268)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4268)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4268
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24915_length_4268_cov_4.254925"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            13..165
                     /locus_tag="PLP95_05930"
     CDS             13..165
                     /locus_tag="PLP95_05930"
                     /inference="COORDINATES: protein motif:HMM:NF012656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HPB45374.1"
                     /translation="MQIHGGMGYAEEYDVSRYWVDARVLSIFEGADETLCLKVIARQL
                     VANHKG"
     gene            complement(216..1427)
                     /locus_tag="PLP95_05935"
     CDS             complement(216..1427)
                     /locus_tag="PLP95_05935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB45375.1"
                     /translation="MSDSDIDSPVRRYVELGLRLGRHNPDIVDSYYGPKQWRDRIDAE
                     PVRPAEELRDAAGELIAEFDADTHLGSARARWLRSQTVGLHTVARHLAGERIEYLDEV
                     ELCYGVRPERITTDEVMAAQHALDRALKGASDEASAPLSQRLAAMRERHVIPVDKLRG
                     VIDNLAQELRARTRAAFGLPDEEGVSFDLVSDKPWSGFNVYQGGFHSLVTINTDLPVL
                     STSIAHLIAHEAYPGHHTEGCRKELGLVRERGQLEQSIALIGTPSCLLAEGLADLGLE
                     ALVGPDTHAATEPMLRDAGVHIELESVQALSVFSETLARARGTLALMLHADAAPVDDV
                     VRTAQQWFVIDEARATQMVRFLTDPTWRAYVFCYAEGHRLCRAFMAGDPRRFARLLDE
                     QFTPQELAAAA"
     gene            complement(1424..2107)
                     /locus_tag="PLP95_05940"
     CDS             complement(1424..2107)
                     /locus_tag="PLP95_05940"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013866.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HPB45376.1"
                     /translation="MGSIVEFPSNGSTARGYYAPAEGGGPGVVVIQEWWGLVPHIEEV
                     CDRFAAAGFSALAPDLYHGETTTEPDEAGKLMMALNIGQAVKDMAGAIAFLQADESVT
                     SEKVGVIGFCMGGGLALVLAASRPDAIGACAPFYGLIPWEGATPDWSSVTAPVRGHFA
                     ELDGFFGPDLARGLEETMRNAGVDAQITIHPGVEHAFFNDHRPEVYNAEASSAAWDST
                     LAFLSDALG"
     gene            complement(2213..2728)
                     /locus_tag="PLP95_05945"
     CDS             complement(2213..2728)
                     /locus_tag="PLP95_05945"
                     /inference="COORDINATES: protein motif:HMM:NF013412.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HPB45377.1"
                     /translation="MAGRRDLIRMNPDEVSEFLGGRRTMNLASYGPDGNIHLVAMWYG
                     FLDSTNVWDRAVGFGDGHGGGEIVIETFAKSQKIKNFQRDNRFTAIVEAGDRYNELQG
                     VELVGRVEILEDRATVVESCKAVLTRYEDFPDDGAITFAAEFAANKRFCVKLHVEKIV
                     SWDHRKLDTDY"
     gene            complement(2795..3589)
                     /locus_tag="PLP95_05950"
     CDS             complement(2795..3589)
                     /locus_tag="PLP95_05950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB45378.1"
                     /translation="MHPIERLRYVARADGSPQSTIVAESARALTAFADDPAGMVAAVQ
                     RLIAHHPTAGAMWWMGGRVLTAPNGAREAFALSEEIEDDPTARELTKALDPEVTVLVV
                     GWPPEIGEALARRGSGRVMVVDTGRESAGLHQFLDHRGVDVLLVPLEGLAAAVRASDI
                     VAIECEAGGPDAGLFGAASASAAAVARCAGVEVWATVGVGRMLPQRLWDALIARWERV
                     DEPWELAEDLVEWTMTDRVVGPGGPIEPHAVTTLTTAALAPELLRF"
     gene            3663..4157
                     /locus_tag="PLP95_05955"
     CDS             3663..4157
                     /locus_tag="PLP95_05955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB45379.1"
                     /translation="MTTLPADLVMHPLGGEDRTAEAQLTLFHLVAVVLDPYTYESSWL
                     LDAAARVLDEFSGADCRTAWVVAADEDGAKSFLGDLATRHLTFADPDREFVKAIGANH
                     LPALVHVALDRSVVGLAEGWNPDTWQPIVDNLARIMSWNAPKVLKSGDPAPYPGTPAV
                     PETV"
BASE COUNT          718 a         1445 c         1395 g          710 t
ORIGIN      
        1 acccgcgagg ccatgcagat ccacggcggc atgggctatg ccgaggagta cgacgtcagc
       61 cgctactggg tggatgcccg tgtgctgtcg atcttcgaag gggccgatga gaccctgtgt
      121 ctcaaggtga tcgcccgcca actcgtcgcc aaccacaagg gctgagcacg gggcctgacc
      181 accaggcctg accaccaggc ctgaccgccc gggcttcacg cagcagcggc gagttcctgt
      241 ggagtgaatt gctcgtcgag cagtcgggca aagcgacgcg ggtcacccgc catgaatgcc
      301 cggcacagcc ggtgaccttc ggcgtagcaa aagacgtacg cgcgccacgt cggatcggtg
      361 aggaagcgaa ccatctgcgt ggcgcgggcc tcgtcgatga cgaaccattg ctgcgccgtg
      421 cgcacgacgt cgtccaccgg agcggcgtcc gcgtgcagca tcaacgccag ggtgccgcgc
      481 gctcgcgcca gcgtctcgga aaacactgac agcgcctgca ccgactcgag ttcgatgtga
      541 acacccgcgt cgcgcagcat cggttcggtg gccgcatggg tgtcggggcc gacgagggct
      601 tcgaggccga ggtcggcaag cccctccgcc aacagacacg agggcgtgcc gatgagcgcg
      661 atcgactgtt ccagttggcc acgctcccgg acgagtccga gctccttgcg acatccctcg
      721 gtgtgatggc ccgggtaggc ctcgtgggca atgagatggg cgatggaggt cgacaacacc
      781 ggcaggtcgg tgttgatcgt gacgagcgaa tggaaaccac cctggtacac gttgaacccc
      841 gaccacggct tgtcgctcac cagatcgaag ctcacaccct cttcgtcggg aagcccgaac
      901 gccgcacggg ttcgggcacg aagttcctgg gcaaggttgt cgatgacccc gcgaagcttg
      961 tcgacaggaa tcacgtggcg ttcgcgcatg gctgccagcc gctgcgacaa cggggcggac
     1021 gcttcatcgg aggccccctt caacgcacgg tcgagcgcgt gctgagcggc catcacctcg
     1081 tcggtggtga tccgctccgg gcgaacgccg taacagagtt cgacttcgtc gaggtactcg
     1141 atccgctccc ccgcaaggtg tcgtgcgaca gtgtgaagtc cgacggtttg gctccgcagc
     1201 cagcgcgccc gagcggatcc gaggtgggtg tcggcgtcga actcggcgat caactcgccc
     1261 gcagcgtcgc gcagttcctc cgcgggccgc acgggctcgg catcgatccg atcacgccac
     1321 tgcttggggc cgtagtagga atcgacgatg tcgggattgt ggcgaccgag gcgaagcccg
     1381 agttcgacgt agcgacgaac cggcgaatcg atgtccgagt cgctcatccg agcgcgtcgg
     1441 acaagaacgc caacgtcgag tcccacgccg ccgacgacgc ctcggcgttg tacacctcgg
     1501 gacgatgatc gttgaagaag gcgtgctcga cacccggatg gatcgtgatc tgggcgtcga
     1561 cgccggcgtt gcgcatcgtc tcttccaacc ctcgggcaag gtcggggccg aagaagccgt
     1621 cgagttcggc gaagtgccca cgcaccggtg cggtgacgct cgaccagtcg ggggtcgcgc
     1681 cctcccacgg gatgagtccg taaaacggtg cgcaggcgcc gatcgcgtcg ggtcgcgatg
     1741 ccgcgagcac gagcgccaag ccgccgccca tgcagaatcc gatgacgccg accttctcgg
     1801 aggtgaccga ctcgtcggcc tgcaggaagg cgatggcgcc ggccatgtcc ttgaccgcct
     1861 ggccgatgtt gagcgccatc atcaacttgc cggcctcgtc gggttcggtc gtcgtctcgc
     1921 cgtggtacag atcgggtgcg agcgcgctga acccggctgc agcgaaccgg tcgcacacct
     1981 cctcgatgtg aggcacgagc ccccaccatt cctgaatgac gacgactccg ggcccgccac
     2041 cttcggccgg ggcgtagtac ccccgtgcgg tcgacccatt gctcggaaat tcaacgatgc
     2101 tgcccatcga cacaacctac cgccgacgat gaccacgcgg cggcgcagct accgaatggt
     2161 tccgctggcg acgacttggt gcgtccgcct ctcgacgaat cgctggaagc tctcagtagt
     2221 cggtgtcgag cttgcggtgg tcccagctca cgatcttttc gacgtgcagc ttcacgcaga
     2281 accgtttgtt ggcagcgaac tccgcggcga aggtgatggc gccgtcgtcc gggaagtcct
     2341 cgtagcgggt gagcacggcc ttgcaactct cgaccacggt cgcacggtct tcgaggatct
     2401 ccacccgtcc gactaactcg acaccctgca gttcgttgta acggtcgccg gcctcgacga
     2461 tggcggtgaa acggttgtcg cgctggaagt tcttgatctt ctggctcttt gcgaaagtct
     2521 cgatgacgat ctcgcccccg ccgtgcccgt cgccgaaacc aaccgcacgg tcccacacgt
     2581 tggtggagtc aaggaacccg taccacatgg ccacgaggtg gatgttgccg tcgggaccgt
     2641 aactggcgag attcatggtt cggcgtccac cgagaaactc ggagacctcg tcggggttca
     2701 tgcggatcag gtcgcgacgt ccggccatcg ggtctccttc aacgtcttgt tggcgcgggc
     2761 tgcgcctcgt cggcagccta cccagttcgc tgcttcagaa ccgaaggagc tccggcgcca
     2821 aagcggcggt cgtcaacgtc gtcacggcgt gcggctcgat cggaccgccg gggccgacca
     2881 cacggtcggt catcgtccat tcgacgagat cctcggcgag ttcccacggc tcgtcgacgc
     2941 gctcccagcg agcgatcagc gcgtcccaga ggcgttgggg caacatccgt ccaacgccga
     3001 ccgtcgccca cacctcgacg ccggcacaac gggcgactgc cgcagcggag gccgacgctg
     3061 caccgaagag cccggcgtct gggcctccgg cctcgcattc gatcgccacg atgtcgctcg
     3121 cccgaaccgc agcggcgaga ccttcgagag gaaccaggag aacgtcgaca ccacgatgat
     3181 cgaggaactg gtgcaggccg gcggactcgc gcccggtgtc gaccaccatg actcgcccgc
     3241 tgcctcgacg ggcgagtgct tcgccgatct ccggaggcca gccgaccacg agtaccgtca
     3301 cctcggggtc gagcgccttg gtgagctcac gagcggtcgg gtcgtcctcg atctcctcgc
     3361 tgagcgcaaa cgcctcgcgg gccccattcg gggcggtgag cacccgccca cccatccacc
     3421 acatggcccc ggcggtcggg tggtgggcaa tcagccgttg caccgcggcg accattcccg
     3481 ccggatcatc cgcgaatgcc gtcagcgccc gggccgactc ggcgacgatt gtcgactgcg
     3541 gtgagccatc ggctcgagcc acgtagcgaa gccgttcgat cgggtgcatg cccggatcgt
     3601 agcgccgcga ccctccgtgc gaatctgcct cacccggtgg aggtgggcta tctttcgatg
     3661 tcgtgacgac ccttcctgcc gatcttgtga tgcaccccct gggtggagag gaccgcaccg
     3721 ccgaagcgca gttgacgctc ttccatctcg tggcggtcgt gttggacccc tatacctacg
     3781 agagttcctg gctgctcgac gccgccgcac gcgtgctcga cgagttttcg ggggccgatt
     3841 gccgcaccgc ctgggtcgtc gctgcggacg aggacggcgc caagtcgttc cttggcgacc
     3901 tggccactcg tcatctcacc ttcgccgacc ccgaccgtga gttcgtcaag gcgatcgggg
     3961 caaatcacct gcccgcgctg gtgcatgtcg cactcgatcg ttcggtcgtc gggctggccg
     4021 aaggatggaa tcccgacacc tggcagccga tcgtcgacaa cctggcacgc atcatgagct
     4081 ggaacgcccc gaaggtgttg aagtccggcg atccggcgcc ctacccgggc accccagcgg
     4141 tccccgaaac cgtctgacct ccgtcggccc tacggtcgac aggtccggcc ggccatcagg
     4201 tccgggctat ggcggcgctt cgcggatgag cggggtgtga gggtcccggg cgcgtagcag
     4261 gggccctg
//
LOCUS       DAOOMA010000175         4267 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_24931_length_4267_cov_3.215337, whole genome shotgun sequence.
ACCESSION   DAOOMA010000175 DAOOMA010000000
VERSION     DAOOMA010000175.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 4267)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4267)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4267
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24931_length_4267_cov_3.215337"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            128..670
                     /locus_tag="PLP95_05960"
     CDS             128..670
                     /locus_tag="PLP95_05960"
                     /inference="COORDINATES: protein motif:HMM:NF012709.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HPB45380.1"
                     /translation="MSASAIATLLASDVSFALPANIAFGITAAVMVYAALRVVTTKNV
                     VHAALWLIVVLLGVAVNFVLLQAEFLAATQVMVYVGAIVVLLLFGVMLTRAPLGVSDD
                     LDNPKAKPGAILIAIVMFVLMAGTSIASWGDDKIGFVTENDIGAVSDSIFGNYLVPFE
                     VVSVLLLAALIGAIVLARKD"
     gene            675..971
                     /gene="nuoK"
                     /locus_tag="PLP95_05965"
     CDS             675..971
                     /gene="nuoK"
                     /locus_tag="PLP95_05965"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012225137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HPB45381.1"
                     /translation="MLLNQFLILSAALFSIGIYGVLVRRNGVLVLISVEMILNSVNIN
                     LVAFGAHHGISGQVFALFVLAVAAAEVGIGLAIVLLIYRNRRSIDFDELDELKG"
     gene            982..3315
                     /locus_tag="PLP95_05970"
     CDS             982..3315
                     /locus_tag="PLP95_05970"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HPB45382.1"
                     /translation="MLHHVWILPALMALSFVLILFFGKRTPGQGHWIGIASIGACLIM
                     SLVIGAQWMQRANEPAHTQAEAFADVNPSCSATAAGFLDGTLHEASHGEEADHAATEA
                     GAEGDHAAGATATAAADGHGRSRAAVSSGSAVSSGSAAAASEGEASYSRPIVKCVHWF
                     NSGGVDIKIGTMLDGASALMLIVVSIVSLLVHVYSTDYVGGDRRYTHYFAFLSLFTAS
                     MMFYVISENTLQMIVGWELVGLCSFALIGHWWEERPNTDAALKAFLTNRVGDVGLLVG
                     MIMLFFLAGDYFGSNGTFNVIDINYAANNGGIEHKMLLIASVALVAAVMSKSGQFILH
                     TWLPDAMAGPTPASALIHAATMVVAGIFMVARLYPVFFDGLAIGGSSINYLAVVGSVT
                     ALFGALMGFVQKDIKKALAYSTVSQLGFMVASLGVGAWTAALFHLFTHAMFKACLFLG
                     AGSLSHAAHHSFNMVDDMGGLRKVMPKTYWTYLIATLSLAGVFPLAGFWSKDEIIAGT
                     GGMNTATGAYTFVLIMLLAAAFCTAAYMTRTIYYAFFGEYRGHGHPHESGPRLTVPLM
                     ILAFLGAVAGFANLPHKFFGLEFGAAAQQFTKFVEPMGDYFPAHAQGFTHAEFNGGLA
                     LLATVIGLAGIAIPAAFYAGKLPFLENLASRNAVAGFFKRILENKYYLDWLYTDVITA
                     FVKGPLARAANWTNQHILDGIVNGAGRYAARTGKWVYSNIDQGVVDGTVNGLGRVASA
                     SGGELRRLQTGNIQQYASLLFAAAALLAGVIVIVVGS"
     gene            3344..>4267
                     /locus_tag="PLP95_05975"
     CDS             3344..>4267
                     /locus_tag="PLP95_05975"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HPB45383.1"
                     /translation="MNTWLNNWSLALVTFLPLVGAALMWLVPKQEEQMHKLIALVASL
                     ATAATGIWIFTNFNYDNGHFQFVIDKSWIEVINARFIMGLDGLSLPLLGLTLAVVPLV
                     IVYSWNHIPSPGNPKAFLSLMLVLETGMIGSFLAEDLVLFFVFFEVVLLPMYFMIGVW
                     GGEDRKYASFKFFIYTMFGSALMLLAFLSLYFLAKDPTTGELARTFDMRALPGLTGGI
                     AHGTQLLIFGGMFVGFGIKVPMFPFHTWLPDAHTQAPTVGSVILAAVLLKLGTYGFVR
                     IAIPLLPQAAVKWAPAIAVLATIGIIYGALGS"
BASE COUNT          691 a         1499 c         1229 g          848 t
ORIGIN      
        1 gcatcgcgat cctggacgtc gacccgcagg gcagcctcgg cgaatggttc gagaccgtgc
       61 cggacttcga accccatgaa gccggatccg agttcaagca gaagcccgtc tcgcgaaagg
      121 gctccaaatg agtgcgtcag cgatcgcaac gctgcttgcg tcagacgtga gcttcgcgct
      181 ccccgccaac atcgcgttcg gcatcaccgc agcggtgatg gtgtacgcag cgttgcgggt
      241 cgtcaccacc aagaacgtcg tgcacgccgc cctgtggctc atcgtcgtgc tgttgggcgt
      301 cgccgtgaac ttcgtgctgc tccaggccga attcctcgcc gccacccagg tgatggtcta
      361 cgtcggcgcc atcgtggtgc tgttgttgtt cggcgtcatg ctcacccggg cacccctcgg
      421 cgtcagcgac gacctcgaca acccgaaggc caagcccgga gccatcctca tcgccatcgt
      481 gatgttcgtg ctcatggccg gcacctcgat cgcctcgtgg ggcgacgaca agatcggctt
      541 cgtcaccgaa aacgacatcg gagcggtctc cgactcgatc ttcggcaact acctggttcc
      601 cttcgaggtc gtctcggtgc tgttgttggc tgcgctcatc ggcgccatcg tcctggcgag
      661 aaaggactga cgccgtgctg ttgaaccagt tcctcatctt gtccgccgcc ctgttctcca
      721 tcggcatcta cggcgtgctc gtgcgccgca acggtgtgtt ggtgctcata tcggtcgaga
      781 tgatcctcaa ctcggtgaac atcaacctgg tcgccttcgg tgcccaccac ggaatctccg
      841 gacaggtatt cgcactgttc gtgttggccg tcgccgctgc cgaggtcggt atcggtctgg
      901 cgatcgtgct gctcatctat cgcaaccgac gctccatcga cttcgacgaa ctcgacgagt
      961 tgaagggctg aggggagagg catgcttcac cacgtttgga tccttccggc gctcatggcg
     1021 ctgtcgttcg ttctgatcct gttcttcggc aagcgcaccc ccggccaggg ccactggatc
     1081 ggcatcgcca gcatcggcgc ctgtctcatc atgtcgctgg tcatcggcgc ccagtggatg
     1141 caacgggcca acgaacccgc ccacacccag gccgaggcct tcgcagatgt gaacccgagc
     1201 tgttcggcca ccgcggcggg attcctcgac ggcaccctgc atgaagcctc acacggcgaa
     1261 gaggccgacc acgcggcgac cgaagcaggc gccgagggcg accacgccgc tggcgcgacc
     1321 gcgaccgccg ctgccgacgg ccacggccgg tcacgcgcgg cagtctcgtc gggctccgcg
     1381 gtctcgtcgg gctccgcggc cgcggccagc gagggcgagg cgtcctacag ccgtccgatc
     1441 gtcaagtgtg tccactggtt caactcgggc ggcgtcgaca tcaagatcgg caccatgctc
     1501 gacggcgcca gcgcgctgat gctcatcgtc gtgtcgatcg tgtcgctgct cgtgcacgtc
     1561 tactccaccg actatgtcgg cggcgaccgt cgctacaccc actacttcgc cttcctcagc
     1621 ctgttcacgg cctcgatgat gttctacgtc atctccgaaa acacgctgca gatgatcgtg
     1681 ggctgggaac tcgtcgggct ctgttcgttc gccctcatcg gccactggtg ggaggagcgg
     1741 cccaacaccg acgccgcgct caaggccttc ctcaccaacc gtgtcggcga cgtcggcctg
     1801 ctcgtcggca tgatcatgtt gttcttcctc gccggcgact acttcgggtc caacggcacc
     1861 ttcaacgtga tcgacatcaa ctatgcggcc aacaacggcg gcatcgaaca caagatgttg
     1921 ctcatcgcca gcgtcgccct cgtcgcggcg gtcatgtcga agtcgggtca gttcatcctg
     1981 cacacctggc tgcccgacgc catggccggc cccaccccgg catcggcact catccacgcc
     2041 gccaccatgg tggtcgcggg catcttcatg gtcgcccgcc tgtatccggt gttcttcgac
     2101 ggcctcgcca tcggcggttc gtcgatcaac tacctcgccg tcgtcggctc ggtcaccgcc
     2161 ctgttcggcg ccctgatggg cttcgtgcaa aaggacatca agaaggcgtt ggcctactcg
     2221 acggtgtcgc agctcggatt catggtggcc tccctggggg tcggcgcctg gaccgcagcg
     2281 ctgttccacc tattcaccca cgccatgttc aaagcctgtc tgttcctcgg cgccggctcg
     2341 ctcagccacg ccgcacacca cagcttcaac atggtcgacg acatgggtgg tcttcgaaag
     2401 gtcatgccca agacctactg gacctacctc atcgccaccc tgtcgctggc gggcgtgttc
     2461 cccctcgccg ggttctggtc gaaagacgag atcatcgccg gcaccggcgg catgaacacc
     2521 gccacgggtg cctacacctt cgtgctcatc atgttgctgg ccgcagcgtt ctgcaccgcc
     2581 gcctacatga cccgcaccat ctactacgcc ttcttcggcg agtaccgcgg ccacggccac
     2641 ccacacgagt cgggtccgcg cctcaccgtg ccgctgatga tcctcgcctt cctgggcgcg
     2701 gtcgccggct tcgccaacct gccccacaag tttttcgggc tcgaattcgg cgccgccgca
     2761 cagcagttca ccaagttcgt cgaaccgatg ggcgactact tcccggccca cgcacagggc
     2821 ttcacccacg ccgagttcaa cggtgggctc gcgctgttgg ccaccgtcat cgggctcgcc
     2881 ggcatcgcca tccccgcagc gttctacgcc ggcaagttgc cgttcctcga gaaccttgcg
     2941 tcacgcaacg cggtcgccgg attcttcaag cggatcctcg aaaacaagta ctacctcgac
     3001 tggctctaca ccgacgtcat caccgcgttc gtcaaaggcc cactcgcccg tgccgccaac
     3061 tggaccaacc aacacatcct cgacggcatc gtcaacggtg ccggccgcta tgcggctcgc
     3121 accggcaagt gggtgtacag caacatcgac cagggcgtcg tcgacggcac ggtcaatggt
     3181 ctcggacggg tcgcaagcgc cagcggtggc gagttgcgca gactccagac cggaaacatt
     3241 cagcaatacg catcgctgct ctttgcagcg gcagcacttc tcgccggcgt catcgtgatc
     3301 gtcgtcggta gttgagccca gtgaggaaga caaggaacct ttcatgaaca cctggctgaa
     3361 caactggagc ctcgctctgg tcaccttcct accgctggtc ggcgcagcac tcatgtggct
     3421 cgtgcccaag caggaagagc agatgcacaa gctcatcgcg ctcgtggcgt cgttggccac
     3481 cgccgcgacc ggcatctgga tcttcaccaa cttcaattac gacaacggac acttccagtt
     3541 cgtcatcgac aagagctgga tcgaggtgat caacgcccgg ttcatcatgg gcctcgacgg
     3601 cttgtcgctg ccgctgctgg gcctgaccct cgcggtggtg ccactcgtca tcgtgtactc
     3661 gtggaaccac atcccgtctc cgggtaaccc caaggcgttc ctgtcgctga tgctcgtgct
     3721 cgagaccggc atgatcgggt cgttcctcgc cgaggacctc gtgttgttct tcgtgttctt
     3781 cgaagtcgtg ctgttgccga tgtacttcat gatcggcgtg tggggcggcg aagaccgcaa
     3841 gtacgcgtcg ttcaagttct tcatctacac gatgttcggc tcggccctca tgttgttggc
     3901 cttcctgtcg ctgtacttcc tcgcaaagga cccgaccacc ggcgaactcg cccgcacctt
     3961 cgacatgcgt gcgcttccgg gcctcaccgg cggcatcgcc cacggcaccc agttgctcat
     4021 cttcggcggc atgttcgtcg gcttcggcat caaggtcccg atgttcccgt tccacacctg
     4081 gctgcccgac gcccacaccc aggccccgac cgtcggttcg gtcatcctgg ccgccgtgct
     4141 gctgaagctg ggcacctacg ggttcgtacg catcgccatc ccgctgctgc cccaggcggc
     4201 cgtgaagtgg gcgccggcca tcgccgtgct ggccaccatc ggcatcatct acggtgccct
     4261 cggttcg
//
LOCUS       DAOOMA010000176         4260 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_24992_length_4260_cov_3.942925, whole genome shotgun sequence.
ACCESSION   DAOOMA010000176 DAOOMA010000000
VERSION     DAOOMA010000176.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 4260)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4260)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4260
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24992_length_4260_cov_3.942925"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1383)
                     /locus_tag="PLP95_05980"
     CDS             complement(<1..1383)
                     /locus_tag="PLP95_05980"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HPB45384.1"
                     /translation="MSIFQPRMLDAAPSMSELCRLVTDLPDGVLLMSPIGEVLWANHA
                     AEQIFQMTFVEAIERGITGLDLLHPDDLALAHAAMTSVQNKEVGTPIEMRVKTPDGWC
                     LCEMVGATKDYGIVLVIRDITYRRRWEIAHDQEARFRALVSNSDVLTLLMTADGLIEQ
                     SSVVVTRLLGHDQETIEGMPFEQIVSEEDRPAWRELLHFLVSGHGHSAESESLDLELL
                     AADGRHVPFLVTVKNLLDDQSVAALVLSGHDISDRVAVERALRGANSVLAATFESSAD
                     GICVTSPAGEITMWNRRFLEVWGFDREVLYSSSRKELHKLFTSQLADPDEFLTSFNDL
                     SERPDAELSDELHFADGRRIERITVPQRVDGEVVGRVWCYRDVTERRQLHEQLTRQAF
                     HDSLTGLANRTLFRQRVELAIGRFRPADAPVAVLFIDLDDFKTVNDSLGHLVGDEMLI
                     SVSNRLNSCLR"
     gene            complement(1555..2154)
                     /locus_tag="PLP95_05985"
     CDS             complement(1555..2154)
                     /locus_tag="PLP95_05985"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HPB45385.1"
                     /translation="MIYLVRHGHTASNASGLLLGHANPSLDDVGRSQAAAVAVSLSGP
                     AGVELTRVVCSPLARTSQTAVEIARVSGLDGPVTDERFIELDYGDWDGRPISSVSAQE
                     WARWRSDAQFAPPGGESLVDLHTRVTAGLADLLEDAQDHDIAIVTHVSPIKSAVAWAL
                     GVDIAVSWRMFVQPAAVTRIALRGGVASLYGFNDVSHLS"
     gene            complement(2210..2713)
                     /locus_tag="PLP95_05990"
     CDS             complement(2210..2713)
                     /locus_tag="PLP95_05990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB45386.1"
                     /translation="MLFWFAATSVAIVWVVFRSPAVDYRMVALGSLIALVETPFGMGP
                     LQSLTGSVVAMIVVMALTVGRRLVRRRYLGIPIGMFLHLVLGGAWTNANAFWWPFAGW
                     SFGDDRSPIVAWGIWSFMLELIGIMVALWLWNEFGLDDPDRRRRFWSTGQLDRAFLRE
                     RGARIDD"
     gene            complement(2720..3433)
                     /locus_tag="PLP95_05995"
     CDS             complement(2720..3433)
                     /locus_tag="PLP95_05995"
                     /inference="COORDINATES: protein motif:HMM:NF014630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-type zinc ribbon domain-containing protein"
                     /protein_id="HPB45387.1"
                     /translation="MDFEALLTLQTLDTRLDQLEYRRTHDETIAARDLAQAAVIEAQA
                     PIEAAEAQRHTVQRELKRFEDEAALTEDKISKHEATLYGGTVTAHKELEAIQAEIAML
                     KKRLSEIEDHELEQMELLEPLDAQIAELSVQRDERAALLDTAERAVTVAMTELEVEVD
                     AARAERSEIAATVDPSLVARYEDLRTRLGGQGAARLAPGGRCEGCHLTLPSAEYSDIK
                     RRPADEIVQCPECDRILVR"
     gene            complement(3544..>4260)
                     /locus_tag="PLP95_06000"
     CDS             complement(3544..>4260)
                     /locus_tag="PLP95_06000"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HPB45388.1"
                     /translation="VSEVAARMGITTIDADFNEVPITENPPINLPLGTREVHAVDMAA
                     AYGAAENDGVYHAPYYIDRITDARGEVLYEHLDPGSRVMSSDTAKLVTEILAKNVTGG
                     TGRRAAIKGQVSAGKTGTTQNNADAWFIGYTPYAVTAVWLGNPTSQDDIVINGRRIQG
                     ADYPSRVWGAFNTAYHQDLESRAFPEAPDTRSGKSIKYRNKYDDGQPKVAATTTTVPG
                     EAPADPGAAATPAASAPDAN"
BASE COUNT          702 a         1399 c         1411 g          748 t
ORIGIN      
        1 gcgaagacac gagttcagcc ggttgctgac cgagatcaac atctcgtcgc cgacaaggtg
       61 cccgaggctg tcgttgacgg tcttgaagtc gtcgaggtcg atgaacagca ccgcgacggg
      121 cgcgtccgcg ggccggaatc gaccgatcgc cagctcgact cgttggcgaa acagcgtgcg
      181 gttggcgaga cccgtcaacg agtcgtgaaa ggcctggcgg gtgagctgtt cgtggagttg
      241 gcgacgctcg gtgacgtccc ggtaacacca gacgcgaccg acgacctcgc cgtcgacccg
      301 ctgcggcacc gtgatacgtt cgatgcgccg cccatcggcg aagtgcaact cgtcgctgag
      361 ttccgcgtcg ggtcgctcgg acaggtcgtt gaacgaggtc aagaactcgt cggggtctgc
      421 gagctgggag gtgaacagtt tgtggagttc cttgcgcgac gaggagtaga gcacctcgcg
      481 gtcgaacccc cacacctcga gaaagcgccg gttccacatg gtgatctcgc cggcggggct
      541 ggtgacgcag attccgtccg ccgaggattc gaaggtggcg gcgagcaccg agttggcgcc
      601 gcgaagcgca cgctcgaccg cgacgcgatc ggagatgtcg tggccgctca gcaccagcgc
      661 tgcgaccgac tggtcatcga gcagattctt cacggtgacg aggaagggca cgtgacggcc
      721 gtccgcggcg aggagttcga gatcgaggct ctccgactcg gccgagtggc cgtgtcccga
      781 cacgaggaag tgcaagagct cccgccacgc cggacgatcc tcctcgctca cgatctgctc
      841 gaagggcatg ccttcgatcg tttcctggtc gtggcccagc aatcgggtga ccacgaccga
      901 actctgctcg atcaggccgt cggcagtcat gagcagggtc agcacatcgg aattggagac
      961 gagcgcacgg aatctcgcct cctgatcatg ggcgatctcc caccgacgtc gataggtgat
     1021 gtcgcggatc accagcacga ttccgtagtc cttggtcgct ccgaccattt cgcacagaca
     1081 ccaaccgtcc ggggtcttga cccgcatttc gatcggggtg ccgacctctt tgttctgcac
     1141 cgaggtcatc gcggcgtgag ccagtgccag gtcgtcgggg tgcaacaggt ccagcccggt
     1201 gatgccgcgt tcgatggctt cgacgaaggt catctgaaag atctgttcgg ccgcatggtt
     1261 ggcccaaagg acctcaccga tcgggctcat caacagcacg ccgtcgggca ggtccgtcac
     1321 gaggcgacag agctccgaca tgcttggtgc tgcatcgagc atgcgcggct gaaagatcga
     1381 cacatcaacc tttcggtagt tcaggggccg acctgacctg ttttgtgcag aacacatcgc
     1441 tcgacccacg cgcgcccgga gccgcggcgg gcgtttcgcg gagccgatcc ggccgagggc
     1501 tccggccaac gccgtcggtg tctgggtcgg tgtctgggtc ggtgtctggc agcttcagga
     1561 caggtgcgac acgtcgttga acccatacag gctcgcgaca ccgccccgca gcgcgatccg
     1621 cgtgaccgcc gccggctgca cgaacattcg ccacgacacc gcgatgtcga cgccgagtgc
     1681 ccacgcgacc gcggacttga tcggcgacac gtgagtcacg atggcgatgt cgtggtcctg
     1741 ggcgtcctcg aggaggtccg ccaaccccgc ggtcacccgc gtgtgcaggt cgaccagcga
     1801 ttcgccaccg ggcggggcga actgcgcatc gctgcgccac cgcgcccact cctgcgcgct
     1861 gaccgacgag atcggtcgcc cgtcccagtc gccgtagtcc agctcgatga accgctcgtc
     1921 ggtgaccggg ccatcgaggc cgctgacccg ggcgatctcg accgccgttt ggctggttcg
     1981 ggccagtggc gagcacacca cccgggtcaa ctcgacgccg gcggggcccg acagtgacac
     2041 ggcgaccgcg gccgcctgcg acctgccgac atcgtcgagc gacgggttgg cgtggccgag
     2101 taggagcccc gacgcattcg aagcggtgtg tccgtgtcga acgagataga tcatcgtggc
     2161 ccctcagtca tccctgcccc ctcaatcatc gatcctcaat catcgatcct cagtcatcga
     2221 tccgtgcgcc gcgttcgcgc agaaaggcgc ggtcgagctg gccggtgctc cagaaccgcc
     2281 gccgtcggtc ggggtcatcg aggccgaact cgttccagag ccacagcgcc accatgatgc
     2341 cgatcagctc caacatgaac gaccagattc cccaggcgac gatcggcgag cggtcgtcgc
     2401 cgaaggacca gccggcgaac ggccaccaga acgcgttcgc gttcgtccag gcaccgccca
     2461 gcaccaggtg caggaacatg ccgatgggga ttccgaggta gcggcgccgc acgaggcgac
     2521 gcccgacggt cagtgccatc accacgatca tcgccacgac cgatccggtg agcgattgca
     2581 gcgggcccat cccgaacggc gtttcgacga gcgcgatcag cgacccgagc gcgaccatgc
     2641 ggtagtcgac tgcagggctg cgaaacacca cccagacgat ggcgacgctc gtcgccgcga
     2701 accagaacag cacgaccgat cagcgcacca gaatgcgatc gcattccgga cattggacga
     2761 tctcgtcggc cgggcgtcgt ttgatgtcgg agtactcggc gctcggcagg gtcaggtgac
     2821 acccctcaca acgtccgccg ggcgcgagtc tcgcagcgcc ttgaccgccg agcctcgtgc
     2881 gcaggtcctc gtagcgggcc accagcgaag gatcgaccgt ggcggcgatc tcgctacgct
     2941 cggcccgtgc agcgtcgacc tccacctcga gttcggtcat cgccacggtg accgcccgct
     3001 cagccgtatc gagtagcgcg gcgcgttcgt cgcgttgaac gctgagctcg gcgatctgag
     3061 cgtcgagcgg ttcgagcaat tccatctgct ccaactcgtg gtcctcgatc tcgctcaacc
     3121 gcttcttcaa catggcgatc tcggcctgta tggcttcgag ttccttgtgg gcggtcaccg
     3181 tcccgccgta cagggtcgcc tcgtgtttgg aaatcttgtc ctcggtgagc gcggcctcgt
     3241 cttcaaaccg cttcagttcc cgctggacgg tgtgacgctg cgcctcggcg gcctcgatcg
     3301 gagcctgggc ctcgatgacc gctgcctgcg caaggtcgcg tgcggcgatc gtctcgtcgt
     3361 gggtgcgtcg gtactccaac tgatccaggc gcgtgtccaa cgtctggagt gtgagcagcg
     3421 cttcgaaatc cacgggacct ccacatggga accagagccc ggtcgggcat cgacctgtcg
     3481 aggaacccta cgcgatcacg gcgagccgcc gacgttggcg gtcgacctca gttggcgcct
     3541 ggctcagttg gcgtccggcg cgctggcggc cggagttgcc gcggccccgg gatcggcggg
     3601 agcctcgccc ggcaccgtgg tcgtcgtggc ggcgaccttc ggttggccgt cgtcgtattt
     3661 gttgcggtac ttgatcgact tgccggagcg ggtgtcgggc gcctccggaa acgcccgcga
     3721 ttccagatcc tggtggtagg cggtgttgaa cgcaccccag acccgcgacg ggtagtccgc
     3781 tccctggatt cggcggccgt tgatgacgat gtcgtcctgt gaggtcgggt tgcccagcca
     3841 cacggcggtg acggcatatg gggtgtatcc gatgaaccag gcgtcggcgt tgttctgggt
     3901 ggtgccggtc tttccggccg acacctgtcc cttgatcgcc gcacgcctgc ccgttccgcc
     3961 ggtcacgttc ttggcgagga tctcggtgac gagtttggcg gtgtccgacg acatgacccg
     4021 acttccaggg tcgaggtgtt catagagcac ctcgcctcgg gcatcggtga tccggtcgat
     4081 gtagtagggc gcgtgataga ccccgtcgtt ctcggcggcg ccataggcgg ccgccatgtc
     4141 gaccgcgtga acctcgcggg tgcccagcgg caggttgatg ggtgggttct cggtgatcgg
     4201 aacctcgttg aagtccgcgt cgatcgtggt gatgcccatg cgggccgcga cctcggacac
//
LOCUS       DAOOMA010000177         4260 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_24993_length_4260_cov_3.501784, whole genome shotgun sequence.
ACCESSION   DAOOMA010000177 DAOOMA010000000
VERSION     DAOOMA010000177.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 4260)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4260)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4260
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24993_length_4260_cov_3.501784"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1430)
                     /locus_tag="PLP95_06005"
     CDS             complement(<1..1430)
                     /locus_tag="PLP95_06005"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR02946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="wax ester/triacylglycerol synthase family
                     O-acyltransferase"
                     /protein_id="HPB45389.1"
                     /translation="MQRLSGIDASFLYLETPSAHMHVCMTGIYDTSTMPNGYSFEAFR
                     DHIVGRFHSIPAFTRRLVEVPFQLHHPMWVEDPDFDPDYHVRRITAPEPGGRRELAAV
                     AAQIASVPLDRTKPLWEVWVIEGLKHDRIGFVAKVHHAAIDGASGAEIMTELYDLEPS
                     PPPRPEVVPTPPEAVPTDIEMILGAFASRLRRTARTVPLVGRTVQSVANVVSNRRDPA
                     GSVGAVPLTAPRTPWSAAISPQRSVAFARIPLDETKVIKDRLGITVNDVVLGVVSGSI
                     RRYLEQRDSLPDTPLVAVCPVSVRHEDEVGAGGNRVSAMFTSLATHLDHPIQRLRTIS
                     SSTEGAKAEHNAIGARMLTEWGEWAAPRTFAMASRLYSSMGLASMHRPIHNLVISNVP
                     GPPFPLYLAGAELVAAYPMGPIMEGSGLNVTVLSYMGHIDFGFLADPLLVPDVWDLAK
                     HVQDAFDELSEIAHRGEPHHTVRAKKA"
     gene            1546..2775
                     /locus_tag="PLP95_06010"
     CDS             1546..2775
                     /locus_tag="PLP95_06010"
                     /inference="COORDINATES: protein motif:HMM:NF019474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HPB45390.1"
                     /translation="MESILRSAIIWLVAVFNAHRPRIRNLPLTIPSFFAGWIVTEAAG
                     LFAAGRVVHIARAWCRGRYRSTQGKVALGLDVGVIAGLAALVAAGRRVDEEMEAALAP
                     HVDRDFVADRPSSVRRGAVFPVMMGASTTKMTRNVGYIDEARWRNTLDIYEPKHGDVV
                     DGESAVAPRPAIIEVHGGGWVIGNKNQQGLALLHHMVKQGWVGFNVNYRLAPKAKFPE
                     QLIDCKRAIAWVRANAERLNIDPNFIVVTGGSAGGYLTAMIALTANDPEFQPGFEDAD
                     TSVAAAVAFYGVYDLVDEDHTMVRLFAEFMEQMVVGSSRVEDRERWDRYSPIRRVRRD
                     APPMLILHGAADTLVPVEQGREFVAALAAVSDNPVIYAELAGANHAFDIFPSPRSVRV
                     VEFVERFLTGLRQGVLR"
     gene            2859..4082
                     /locus_tag="PLP95_06015"
     CDS             2859..4082
                     /locus_tag="PLP95_06015"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HPB45391.1"
                     /translation="MHRLISRLGFSSYEELHSWSVEQPEGFWSTMWDELAVIGDRGEP
                     VVVEGETMRSARFFPQARLNVAENILRGGPGPDDDEAFILSLDERGGPALTLTRGQAR
                     AQVARISAALRADGVGVGDVVAAWMPNVAETVLVMLAAASIGATFTSTSSDFGVDGVL
                     DRFTQVNPTVLVSADGYRYNGKELDCLQRLGEIVAGLPSLRRTVVVSVLGVGEANKPG
                     TGLTRYEDWLEPHEDAQLRFEALGFDHPWYVLYSSGTTGVPKCIVHRAGGVLLQHLKE
                     HRFHAGIEAGDRVFYFTTCGWMMWNWLVSAPALGAAIVLYDGSPFHPGPQRLWELAQR
                     EGFTFFGTSAKYLDSIRKAGFDPRQVGDLADLRAMASTGSPLSPETNAWAVAHLPRRS
                     TWGRSRVARICARPS"
BASE COUNT          708 a         1322 c         1460 g          770 t
ORIGIN      
        1 gccttcttcg cgcggacggt gtggtgcggc tcgccgcgat gtgcgatctc gctcaactcg
       61 tcgaacgcat cctgaacgtg cttggcgagg tcccagacgt cggggaccag gagcggatcg
      121 gcgaggaagc cgaaatcgat gtggcccatg taactgagca ccgtgacgtt gaggcccgaa
      181 ccttccatga tcggacccat cggatacgcg gcgacgagtt cggcgcccgc gagatacaac
      241 gggaacggtg gtccgggaac gttggagatg acgaggttgt ggatcgggcg gtgcatgctc
      301 gccaggccca tcgacgaata caggcgggag gccatggcga acgtacgagg cgctgcccat
      361 tccccccact cggtcaacat gcgtgcaccg atcgcgttgt gttccgcctt cgctccctcc
      421 gtcgagctgg aaatcgtgcg gagccgttga atcggatggt ccagatgggt cgccagcgag
      481 gtgaacatgg cagaaacgcg gttaccgccg gcgcccacct cgtcctcgtg gcgaaccgag
      541 accggacaca ccgccaccag cggcgtgtcc gggagtgagt ctcgttgttc gagatagcgg
      601 cgtatcgaac ccgacacgac cccgagcacg acatcgttga cggtgattcc caaacggtcc
      661 ttgatcacct tggtctcgtc gagcggaatg cgcgcgaacg cgacacttcg ctgcggggag
      721 atcgccgcgc tccacggcgt gcgcggcgcc gtcaacggca cggcgccgac cgaacctgcg
      781 gggtcgcgcc ggttgctgac gacgttcgcg acgctttgca ccgtgcgccc cacgagcggc
      841 acggtgcgtg ccgtgcgccg cagtcgcgaa gcgaacgcac cgaggatcat ctcgatatcg
      901 gtgggcacgg cctcgggtgg ggtgggcacg acctcgggtc ggggtggcgg cgacggttcg
      961 aggtcgtaca gctcggtcat gatctccgcg ccggaggccc catcgatcgc agcgtggtgg
     1021 accttggcga cgaagccgat gcggtcatgc ttcaggccct cgatgaccca gacctcccag
     1081 agcggtttgg tccggtcgag tggcaccgac gcgatttggg ctgcgaccgc cgcgagttcg
     1141 cgtcgcccac ctggctcggg ggcggtgatg cggcggacgt gatagtcggg atcaaaatcg
     1201 ggatcctcga cccacatcgg atggtgcagc tggaacggaa cttcgacgag gcgtcgggtg
     1261 aaggccggaa tcgaatggaa ccgcccgacg atgtgatcgc gaaatgcttc gaacgagtag
     1321 ccgttgggca tggtggaggt gtcgtagatc ccggtcatgc agacgtgcat gtgagcactc
     1381 ggcgtctcca ggtaaagaaa tgaagcatcg attcctgaca gtcgctgcat gagcccccct
     1441 ctaggttcgg agcctgattc tgccagcgtt tcgaccttgc gggacgaaga acatgcgaga
     1501 aattctcgag ccgcgaactt cgttccggtc cgcgaagatg gggcgatgga atcaatcctg
     1561 cgttcagcga tcatctggtt ggtcgcggtg ttcaatgccc accgccctcg catccgcaac
     1621 ctccccttga cgatcccgtc gttttttgct ggctggatcg tcaccgaggc cgccggactg
     1681 ttcgccgccg gacgggtcgt ccacatcgct cgagcctggt gtcgcggtcg ctatcgcagc
     1741 actcagggca aggtggcgct ggggctggat gtcggggtga tcgccggatt ggcggcgctc
     1801 gtcgctgcgg ggcgtcgcgt cgatgaggag atggaggcgg cgttggctcc ccacgtcgac
     1861 cgggactttg tggccgaccg cccctcttca gtgcgaaggg gtgcggtgtt tccggtgatg
     1921 atgggggcgt cgaccacgaa gatgacgcgc aatgtcggat atatcgatga ggcgcgctgg
     1981 cgcaacaccc tcgatatcta cgaaccgaaa cacggcgacg tcgtcgatgg cgagtccgct
     2041 gtggcacccc ggccggcgat catcgaggtg cacggcggcg gatgggtgat cggcaacaag
     2101 aaccaacagg gcctggcgct gctacatcac atggtcaagc aggggtgggt cgggttcaac
     2161 gtcaactacc gcctcgcgcc gaaggccaag ttccccgaac aactcatcga ctgcaagcgg
     2221 gcgatcgcct gggtgagggc gaacgccgaa cgcctcaaca tcgatcccaa cttcatcgtt
     2281 gtgaccggtg ggtccgccgg aggctatctc acggcgatga tcgcgttgac cgcgaacgat
     2341 cccgagttcc aaccgggctt cgaggatgcc gacaccagcg ttgccgccgc cgtcgcgttc
     2401 tacggggtct acgacctggt cgacgaggat cacacgatgg tgcgattgtt cgccgagttc
     2461 atggaacaga tggtggtggg ttcgtcacgc gtcgaagatc gcgagcgttg ggaccgctac
     2521 agcccgatcc gtcgcgtgcg ccgggacgcg ccaccgatgc tgatcctcca cggagccgcc
     2581 gacaccctcg ttccggtcga acagggccgc gaattcgtcg cagcgctcgc cgcggtctcc
     2641 gacaacccgg tcatctacgc cgaactagcc ggagcgaacc acgccttcga catcttccct
     2701 tcgcctcggt cggttcgtgt cgtcgagttc gtcgaacggt tcctcacggg gctccgtcag
     2761 ggcgtgctgc ggtgaatcgg gcgagtggtc aagctgtcga gggcgacgat ctggagccga
     2821 tctggtggcc gtcgaacgac gcggttcgcc ggtcggcaat gcatcggttg atctcgcggt
     2881 tggggttctc gagctacgag gagttgcact cctggtcggt tgaacagccc gaggggttct
     2941 ggtcgacgat gtgggacgaa ctcgcggtga tcggagatcg cggtgaaccc gtcgttgtcg
     3001 agggcgagac gatgcgttcg gcgcggttct tcccgcaggc tcgactcaac gttgccgaga
     3061 acattcttcg cggcggacca gggcccgatg acgatgaggc gttcatcctg agcctcgacg
     3121 agcgcggtgg cccggcgctc acgttgaccc gcgggcaggc ccgagcgcag gtcgcgcgga
     3181 tctcggcggc gttgcgagcc gatggggtcg gtgtcggcga tgtggtcgcg gcgtggatgc
     3241 ccaacgttgc cgaaaccgtg ctggtgatgt tggcagcggc gtcgatcggc gcaaccttca
     3301 cctccacctc ctcggacttc ggtgtcgacg gggtgctcga tcggttcacc caggtgaacc
     3361 cgacggtgct cgtctccgcc gacggctacc ggtacaacgg caaagaactc gattgtcttc
     3421 aacgcctcgg cgagatcgtc gccgggttgc cctccctgcg ccgaacggtg gtggtctcgg
     3481 tgctcggagt cggcgaggcc aacaaacccg ggacgggcct cacgaggtac gaggactggc
     3541 tggagccgca tgaggatgcg cagcttcgct tcgaggcgct tggtttcgat cacccctggt
     3601 acgtgttgta ctcctcgggc acgaccgggg tgcccaagtg catcgtgcac cgagccgggg
     3661 gtgtgctgtt gcaacacctc aaagagcacc ggttccacgc cgggatcgag gcgggcgaca
     3721 gggtattcta cttcacgacc tgcggttgga tgatgtggaa ctggctggtc tccgcgccgg
     3781 cgctcggcgc agcgatcgtg ttgtacgacg ggtcgccgtt tcaccccggc ccccaacgcc
     3841 tctgggaact cgctcagcgc gaggggttca ccttcttcgg aacttcggcg aagtacctcg
     3901 attccattcg aaaggccggc ttcgacccgc gacaggtcgg agacctcgcc gatctgcgcg
     3961 ccatggcatc gaccggatcg ccactgtcgc cggagacgaa cgcatgggcc gtcgcccacc
     4021 tcccccggcg gtccacctgg ggtcgatctc gggtggcacg gatctgtgcg cgaccttcgt
     4081 agccggcgac ccgagcgccc cggtgtatgc cggcgtgatc caacgtccga ccctcgcgat
     4141 ggcggtggcg gtgctcgacc ccgacggcca tgaggtggcg gccggcgagc gcggcgaact
     4201 gtgctgcacg gcaccgttcc cgtcgatgcc gttggggttt gcgaacgatc ccgatggcga
//
LOCUS       DAOOMA010000178         4211 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_25381_length_4211_cov_3.865014, whole genome shotgun sequence.
ACCESSION   DAOOMA010000178 DAOOMA010000000
VERSION     DAOOMA010000178.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 4211)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4211)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4211
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25381_length_4211_cov_3.865014"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..268)
                     /locus_tag="PLP95_06020"
     CDS             complement(<1..268)
                     /locus_tag="PLP95_06020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012224024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HPB45392.1"
                     /translation="MTDTASNGAAPQSHAEMLNLTLKVWRQEGPSAAGHFETYTTSIA
                     DDASFLEMLDYVNEDLIDHDKEPITFDHDCREGICGTCGLMINGQ"
     gene            complement(278..2212)
                     /locus_tag="PLP95_06025"
     CDS             complement(278..2212)
                     /locus_tag="PLP95_06025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase/succinate dehydrogenase
                     flavoprotein subunit"
                     /protein_id="HPB45393.1"
                     /translation="MAFTLDSAIPPGPLADKWESHKFTTKLVNPANRRKFEVIVVGTG
                     LAGGAAAATLGELGYNVKVFTYHDSPRRAHSIAAQGGINAAKNYRGDGDSIHRLFYDT
                     IKGGDFRSREANVHRLAEVSLNIIDQCVAQGVPFGREYSGLLDNRSFGGAQVARTFYA
                     RGQTGQQLLLGAYQALARQIHAGTCTLYNKMEMIELVVGDDGVATGIIARDLLTGELS
                     RHSAHAVVLATGGYGNVYFLSTNAKNSNATAAWRAHRKGAAFANPCYTQIHPTCIPPS
                     DEFQSKLVLMSESLRNDGRIWVPKNAADCGKDAASIAEDDRDYYLERKYPAFGNLVPR
                     DIASRNAKTMVDQGLGIGPLRNGVYLDFAESIGRLGEDTIKARYGNLFDMYERITGEN
                     PYKVPMRIYPATHYTMGGLWVDYNLMTTVPGLFAIGEANFSDHGANRLGASALMQGLA
                     DGYFVLPPTIAGFLAPQLGTAPMATDSAAFDRAEADARGRIDMLMGAKGTHSVDHYHR
                     ELGKIIWDYCGMARNSTDLGKALSEIPVLREEFRRDVKILGTAEGVNQSLEKALRVDD
                     FMELAELKVRDALHREESCGGHFREEHQTPEGEAKRDDENFMYVGAWEYGGPNIQTDA
                     VLHKEPLEYEVVEPTQRSYK"
     gene            complement(2232..2993)
                     /locus_tag="PLP95_06030"
     CDS             complement(2232..2993)
                     /locus_tag="PLP95_06030"
                     /inference="COORDINATES: protein motif:HMM:TIGR02046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase cytochrome b subunit"
                     /protein_id="HPB45394.1"
                     /translation="MAQTMNRPGAQARRVNPERKPAPWPVEFYRSAVGKKWAMALSGV
                     AMLGFVLAHAIGNLKLYMAPHDGEAALDGYAEFLRRLLYPLAPEGVVLWLMRIGLLVA
                     LIVHVHAAITLTQMNRRARPMKYQSPRDYVAVNFASRSMRVTGFVLLTYIIFHVLDLT
                     IGTTNGGFVAHHVHNNLVASLERPWAAVIYIVANLAISVHLYHGIWSVFQSTGLNNPR
                     YNTARRTVATAIAALIGVVNVLFPIMVLAGVVERV"
     gene            complement(3175..3864)
                     /locus_tag="PLP95_06035"
     CDS             complement(3175..3864)
                     /locus_tag="PLP95_06035"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HPB45395.1"
                     /translation="MPTDQPNEAPNPEGFRQHRYRPPSGATRLLLVRHGESEPAVRGQ
                     RFPLVDGHADPALHPDGRAQAQRVGERLGVESDIAGIWVTTLRRTHQTAAPLAKRLGI
                     EPQVAADLREVHLGDWEGGELRWRAADRDPLYLQVIEQRRWDIIPGAEPRAEFAARLR
                     RGVAMITTAHRGETVVAVVHGGVIGQLLGDAVGADPFAFSGADNASISELIIDGDRQV
                     LRRFNDTAHLG"
     gene            complement(3922..>4211)
                     /locus_tag="PLP95_06040"
     CDS             complement(3922..>4211)
                     /locus_tag="PLP95_06040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB45396.1"
                     /translation="HGGEPLALDLTVVNDSEERVEGEVEALLEWNGDHHRWGARVGVD
                     PDRSVVVATPQFVVPAITGPLSLTLNLHLSDGSPAIVSRYTGEIFADPHEH"
BASE COUNT          706 a         1325 c         1428 g          752 t
ORIGIN      
        1 cctgaccgtt gatcatcaga ccacacgtgc cacagatgcc ctcacggcag tcgtggtcga
       61 aggtgatcgg ctccttgtcg tggtcgatca ggtcttcgtt cacgtagtcg agcatctcga
      121 gaaacgacgc atcatcggcg atggaggtcg tgtaggtctc gaaatggccg gccgccgaag
      181 ggccctcctg acgccacacc ttgagcgtca ggttcaacat ttcggcgtga ctctggggtg
      241 cagcgccgtt ggatgcggta tcggtcatcg gtgactccta cttgtagctg cgctgggtcg
      301 gctcaaccac ttcgtactca agcggttcct tgtgcaagac ggcgtctgtc tggatgttcg
      361 gaccgccgta ctcccacgcc ccgacataca tgaagttctc gtcgtcgcgc ttggcctcgc
      421 cctcaggggt ctggtgttcc tcacggaagt gaccgccgca ggactcctcg cgatgcagtg
      481 cgtcgcgcac cttcaactcg gcgagttcca tgaagtcgtc gacacgcaac gccttttcaa
      541 gcgactggtt cacgccctcg gccgtaccga gaatcttgac gtcgcggcgg aactcctcgc
      601 gcagcaccgg gatctcgctg agcgccttgc ccagatcggt ggagttgcgg gccatgccgc
      661 agtaatccca gatgatcttg ccgagctcac ggtgatagtg gtcgaccgag tgcgtgccct
      721 tggcgcccat caacatgtcg atgcggccac gggcatccgc ctcggcgcgg tcgaacgcgg
      781 cggaatcggt cgccatcggg gccgttccca actgcggggc gaggaacccg gcgatggtgg
      841 gaggcaacac gaagtagccg tcggccagac cctgcatgag cgccgaggca cccaaacggt
      901 tggcgccgtg atcggagaag ttcgcctcgc cgatcgcaaa caggcccggc actgtggtca
      961 tgaggttgta gtccacccac aggccaccca tcgtgtagtg ggtggcgggg tagatgcgca
     1021 tcggaacctt gtaggggttc tcgccggtga tgcgctcgta catgtcgaac aggttgccgt
     1081 agcgggcctt gatcgtgtcc tcgccgagac ggccgatcga ctcggcgaaa tcgagataca
     1141 cgccgttgcg cagcggaccg atgccaaggc cctggtcgac catcgtcttc gcattgcggg
     1201 acgcgatgtc tcgaggcacc aggttgccga acgccgggta tttgcgctcg aggtagtagt
     1261 cgcggtcgtc ctcggcgatc gaggcggcgt ccttaccgca gtcggcggcg ttcttcggca
     1321 cccagatgcg gccgtcgtta cgcagcgact ccgacatcag cacgagcttc gactggaact
     1381 cgtccgacgg cggaatgcag gtcgggtgaa tctgcgtgta gcaggggttg gcgaaggccg
     1441 cgcccttgcg gtgcgcacgc cacgccgcgg tggcgttgga gttcttggcg ttcgtcgaca
     1501 ggaaatagac gttgccgtag ccgccggtgg cgagcaccac ggcgtgggcc gagtggcggc
     1561 tgagttcgcc ggtgagcaga tctcgggcga tgatgccggt cgccacgccg tcgtcgccga
     1621 cgacgagttc gatcatttcc atcttgttgt agagggtgca ggtgcccgca tgaatctggc
     1681 gggcgagcgc ctggtaggcc cccaacaaca gctgctggcc ggtctggcca cgggcataga
     1741 acgtgcgggc cacctgggcg ccgccgaagg aacggttgtc gagcagaccg ctgtactcgc
     1801 ggccgaacgg aaccccctgg gcgacgcact ggtcgatgat gttcagcgac acctcggcga
     1861 ggcggtgcac gttggcctcg cgggagcgga agtcgccgcc cttgattgtg tcgtagaaga
     1921 ggcgatggat gctgtcgccg tcgccgcggt agttcttcgc cgcgttgatg ccaccctgag
     1981 cggcgatcga gtgcgcccga cgaggggagt cgtgatacgt gaagaccttg acgttgtagc
     2041 cgagctcacc gagggtggcc gccgccgcgc caccggcaag acccgttccg acgacgatga
     2101 cctcgaactt acgacggttc gcggggttga cgagcttggt cgtgaacttg tgcgactccc
     2161 atttatcggc gagcgggccg gggggaatcg cggaatcgag cgtgaatgcc atgtgtgttc
     2221 cttattcgct ctcagactcg ttcgaccacg ccggcgagga ccatgatcgg gaagaggacg
     2281 ttgacgacgc cgatcaacgc ggcgatcgcg gtggcgaccg tgcgccgggc ggtgttgtag
     2341 cgagggttgt tcaacccggt cgactgaaac accgaccaga tgccgtgata caggtgcacc
     2401 gagatggcga ggttggcaac gatgtagatc accgcagccc acggccgctc gagcgaggcc
     2461 accaagttgt tgtgcacgtg atgggcgacg aacccgccgt tggtggtgcc gatcgtgagg
     2521 tcgagaacgt ggaagatgat gtaggtcaac aggacgaacc cggtgacccg catcgaacgg
     2581 gacgcgaagt tcaccgccac gtagtcgcgc ggcgactgat acttcatcgg gcgggcccgg
     2641 cggttcatct gcgtgagggt gatcgccgcg tgcacgtgca cgatgagcgc caccagcaac
     2701 ccgatgcgca tcagccacaa caccacaccc tcgggggcga gcgggtagag cagacgccgg
     2761 aggaactccg cgtagccgtc gagcgccgct tcgccgtcgt ggggcgccat gtagagcttc
     2821 aggttgccga tcgcgtgtgc cagcacgaag ccgagcatcg cgacacccga aagcgccatc
     2881 gcccacttct taccgaccgc acttcggtag aactcgacag gccacggtgc gggcttgcgt
     2941 tcagggttga cgcggcgggc ctgtgcgccg ggtcgattca tcgtttgcgc catgtgggca
     3001 tgctcccagt cgacggttcg tacgacatga acctaccccc gcacgcgagg cgcgcggaaa
     3061 ctcgcaatcg aggccttcaa ggttaggcat gcctaagttc ccgggggatt cgcggcgctg
     3121 agccggctca cagtcggccc tcggccggcc ctcagccggc ggtgcggttg gcggtcaccc
     3181 gaggtgggcg gtgtcgttga aacgccgcag cacctggcgg tcgccgtcga tgatcaattc
     3241 cgagatcgac gcgttgtcgg cgccgctgaa cgcgaacggg tcggccccga ccgcatcgcc
     3301 gagcaactgt ccgatcaccc cgccgtgcac caccgccacc accgtctcac cacgatgcgc
     3361 cgtggtgatc atcgccacgc cgcgccgcag gcgagccgca aactccgccc gcggttccgc
     3421 cccggggatg atgtcccaac gccgctgctc gatgacctgc agatacagcg ggtcgcgatc
     3481 cgcagcgcgc caacgcaact caccgccctc ccagtcgccg agatggacct cgcgtaggtc
     3541 tgcggcgacc tgcggttcga tccccaggcg cttcgcgagc ggtgccgcgg tttgatgggt
     3601 tcgacgcagt gtcgtcaccc agattccggc gatgtcggac tcgacgccga gccgttcgcc
     3661 cacccgctgc gcctgcgccc gaccgtcggg gtgcaatgcc ggatccgcat ggccgtcgac
     3721 gagaggaaat cgttggcccc gaaccgcagg ctcgctttcc ccgtggcgaa ccagcaacag
     3781 gcgtgtcgcc cccgacggtg ggcggtaacg gtgttggcgg aatccttctg ggttgggtgc
     3841 ttcgttgggc tggtcggtgg gcatcggcgc gttctcgagt cgtgggaggt cagtgttcgt
     3901 ggggatcagt gttcgtgggg atcagtgttc gtggggatcg gcgaagatct cgccggtgta
     3961 gcgcgaaacg atcgccggcg acccgtcgct caggtgaagg ttcagcgtga ggctgagcgg
     4021 tccggtgatg gcaggcacca cgaattgtgg ggtggcgacc acgacggaac gatccggatc
     4081 gaccccgacc cgggcacccc agcggtggtg gtcgccgttc cattcgagca gcgcctcgac
     4141 ttcgccctcg acccgctcct ccgaatcgtt cacgacggtg aggtcgagcg ccaacggctc
     4201 gccgccgtgg a
//
LOCUS       DAOOMA010000179         4180 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_25611_length_4180_cov_2.864000, whole genome shotgun sequence.
ACCESSION   DAOOMA010000179 DAOOMA010000000
VERSION     DAOOMA010000179.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 4180)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4180)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4180
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25611_length_4180_cov_2.864000"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1678
                     /locus_tag="PLP95_06045"
     CDS             <1..1678
                     /locus_tag="PLP95_06045"
                     /inference="COORDINATES: protein motif:HMM:NF015211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="terminase family protein"
                     /protein_id="HPB45397.1"
                     /translation="RCSLAAIAGRDLEHFRERVAEAGCAIESETRNPESGNWAVEVSL
                     DDGGTLQTALDRCGLLGNKHVPDEYLRASPAQRLALLRGLMDSGGVNDKTGGKVSFVN
                     QNRRLADAVAELMSSLGTKVSRGRRPVRVGGVETGAIAYRVATMCTFNPFSLPRKAER
                     WSDDSKGSLTRRTRVIAGFETVGTRRVRCIAVDSPSHLFLAGEQMVPTHNSELTTKWN
                     VVWYLGMFEAKRILIVCASGDLAEKFSREARDIFKTWGPLLFGKKVREDMSAVDEWGT
                     SDGGFVRAVGAESKGITGYGFDLIVIDDPVSDAKAARSEVMKKNLLEWYSGTLRTRLQ
                     PGGTMVLVMARWTDDDLSGTVVEKAVREGTGDPWKIIKISAIARCPLPEPDPDDPDFA
                     ALSLERSEWRDEWGRSDGDSFWPEMWPAEVLERIAEGLPDPTAWDALYQQDPVAKSGN
                     AFERGHWRRVDPLDVAIVDRVRAWDLASSENKGDWTVGALLGRTSDRRTVILDIVKGR
                     WSPDRVEAEVLAAADRDGMEVRIRMETPKGDSGVTALHYENLLHHRDFAG"
     gene            1682..2011
                     /locus_tag="PLP95_06050"
     CDS             1682..2011
                     /locus_tag="PLP95_06050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB45398.1"
                     /translation="MKGKKTERAAIMSGSHKKGKLLIALGAAWDEALVAEFERFPYGS
                     HDDQVDACSLAFNQLWEFGGGDTTMVAPTDIDAAIRLIGQYEPPSELVEYQGSLPSPV
                     AADHRTE"
     gene            complement(2513..3424)
                     /locus_tag="PLP95_06055"
     CDS             complement(2513..3424)
                     /locus_tag="PLP95_06055"
                     /inference="COORDINATES: protein motif:HMM:NF039495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB45399.1"
                     /translation="MSRPVRPISEVARGRPQEAGKIKIGKLASTSSGRMRPASIDTFR
                     FTTRDREQVDRLAAAYGGDVVTAPDGSFDLETTSNEINVILPPDPLGGTPIYELWSGG
                     GCARRCDGEEVTVPIRTGDDVAFVDQPCICAMNDSMECKPTVRLSVIIPETSLTGVWN
                     LRTNSWAATREMSQMASLIDMAQSRGLVSATLAVERREQMSAGRKRKFVVPVLRLKAT
                     LEELADGGGVATIGPAPTPAAALPAPRSLSPDDRARLNAAWASASDADRKDAMDLLDR
                     HGITSAAGVTPDLVEDVIDALVVAEPS"
     gene            complement(3444..3740)
                     /locus_tag="PLP95_06060"
     CDS             complement(3444..3740)
                     /locus_tag="PLP95_06060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB45400.1"
                     /translation="MGDEIITRTEVHQFRPEAPAAETAAGISAAVQRFLSDPLVMSSP
                     ESLRIEVRWRYVHDDSSGEWFDGLELTASASVHRANIDVDGTLSVSDDSPVEWG"
     gene            3975..>4180
                     /locus_tag="PLP95_06065"
     CDS             3975..>4180
                     /locus_tag="PLP95_06065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB45401.1"
                     /translation="MPDANLGEPRTPHLPAPRRQRLRHATLQGQRAWTSGRAAGGSTG
                     IVQSSLHLPQYGAEPVDHSEQEPLR"
BASE COUNT          744 a         1416 c         1340 g          680 t
ORIGIN      
        1 acggtgctcg ctggccgcga tcgccgggcg cgacctggag cacttccgtg aacgggtcgc
       61 tgaggccggg tgcgccatcg agtcggaaac ccgcaacccg gagtcgggca actgggcagt
      121 cgaggtttcg ctggacgacg ggggcacgct tcaaacagcc ctcgaccgat gcggactcct
      181 cggcaataag cacgttccag acgagtatct tcgtgcttct ccagcgcagc gcctcgccct
      241 ccttcggggc ctcatggact ccggcggcgt caacgacaag accggcggga aggtgtcgtt
      301 tgtgaaccag aaccgccgtc tcgccgacgc tgtcgccgaa ctgatgtcga gcctcggaac
      361 caaagtgtct cgcggtcggc gtccggtgag ggtcggcggc gttgagacgg gggcgatcgc
      421 ctatcgggtg gcgaccatgt gcacgttcaa cccgttctca ctgccacgaa aagcggagcg
      481 gtggtcagac gactcgaaag gttcgctgac ccgccggacc agggtgattg ccgggttcga
      541 gacggtgggc acgcggaggg tgcggtgtat cgccgtcgac tccccgagcc acctgtttct
      601 ggccggggag cagatggtcc cgacccacaa ctccgagctg acgacgaagt ggaacgtcgt
      661 ctggtatctc ggcatgttcg aggcgaaacg catcctgatc gtgtgcgcgt ccggggacct
      721 cgccgagaag ttctcccgcg aggcccgcga catcttcaaa acgtgggggc ctctcctgtt
      781 cggaaagaaa gtccgcgaag acatgtcggc ggtcgacgag tggggaacga gcgacggagg
      841 gttcgtccga gccgtcgggg ccgaatcgaa aggcatcacc ggatacggtt tcgacctcat
      901 cgtgatcgac gacccggtga gcgacgcgaa ggccgcccgg tccgaagtga tgaagaagaa
      961 cctgctggaa tggtattcgg ggacgctgag aacgcgtctc cagcctggcg ggacgatggt
     1021 tctggtgatg gcacggtgga ccgacgacga cctgtccggc accgtcgtcg agaaagcggt
     1081 ccgcgaaggc accggggacc cgtggaagat catcaagatc agcgcgatcg cccgctgtcc
     1141 cctccccgaa ccagaccctg acgacccgga tttcgccgcg ctctcacttg agcgctccga
     1201 gtggcgtgac gaatggggcc gctctgacgg ggacagcttc tggccggaaa tgtggcccgc
     1261 cgaggtcctc gaacggatcg ccgaagggct ccccgacccg acagcgtggg atgccctcta
     1321 ccagcaggac ccggtagcga aatccgggaa cgcgttcgag cgcggccact ggcggcgcgt
     1381 cgacccgctc gacgtcgcga tcgtcgaccg ggtcagggcg tgggacctcg ccagctcaga
     1441 gaacaaaggc gactggactg tcggggcgct cctgggtcgc acaagcgacc gtcgcaccgt
     1501 catcctcgac atcgtgaaag gcaggtggtc ccccgaccgc gtcgaggccg aagttctcgc
     1561 cgcagcggac cgcgacggca tggaggtccg catccgcatg gaaaccccga aaggcgattc
     1621 cggggtcacg gcgctgcact acgagaacct gctccaccac cgcgacttcg ctggatagcc
     1681 ggtgaagggc aagaaaacgg agcgggcggc gatcatgtcg ggttcccaca agaaagggaa
     1741 actcctgatc gccctcgggg cagcgtggga cgaagccctc gtcgccgagt tcgagcggtt
     1801 cccgtacggt tcacacgacg atcaggttga cgcctgctcc ctcgcgttca accagttgtg
     1861 ggagttcggc gggggcgaca ccaccatggt cgctccgacc gacatcgacg ctgcgatccg
     1921 gctcatcgga cagtacgaac cgccctctga gctggtcgaa taccaaggtt ctctgccgtc
     1981 tccggtggca gcggatcacc ggacggaatg aacgagtaga acacgtaccg gcgcatccgc
     2041 cgcggtgtcc atatctgcat cggccacaac gtcccctcgg cgacaacccc gccgggacac
     2101 agggaactca aaggccagta cggccagtcg tcgtcgacgt tcaacctgtt cccctggaac
     2161 gcccggtacg cttgggggag ggcagaggtg ccgatgtcgc cttggaacgc caccgagttg
     2221 cgcttgaggt acaaccctct ccgctgaaac ctgcgagagt tgcgtcgcat caactgccgc
     2281 tggttgccgt ccatccacga caccgggcgt tcggcgaccc agcgtgccgc cacgtcgacc
     2341 gtttcgaggt cgatctcgac ccaggcgcgc ccgcccacga tccactgaga gtcctctgtt
     2401 tggcgttgaa cgagcgcggc gtacgtctcc tgcgtttcga gcgggtcccc gctgtcctgc
     2461 ccgtacccgg cttgaaggta cagctcagcc cacgtcgtcc cgacagacaa cctcacgacg
     2521 gctcagcgac gaccagggcg tcgatgacat cctcgacgag gtcaggcgtc acccccgcgg
     2581 ccgaggtgat cccgtgacgg tcgagaaggt ccatggcgtc cttgcggtca gcgtccgacg
     2641 ctgacgccca cgcggcgttc aggcgggccc tgtcgtcagg cgacagggag cgaggagctg
     2701 gcagcgccgc ggcaggcgtg ggggccggtc cgatcgtcgc caccccgccg ccgtcggcga
     2761 gttcttcaag tgtcgccttc aaccggagca cggggaccac aaacttgcgt ttccgaccgg
     2821 ccgacatctg ttcgcggcgc tccacggcga gcgtcgcgct cacaaggcca cgcgactgcg
     2881 ccatgtcgat cagggacgcc atctgcgaca tttcgcgtgt cgccgcccac gagttcgttc
     2941 gcaggttcca caccccagtc aatgacgtct ccgggatgat caccgacaat cgaacggtcg
     3001 gcttgcactc catcgagtcg ttcatcgcgc agatgcacgg ctggtcaacg aacgccacat
     3061 catccccagt gcggatcggg acagtcacct cttcgccgtc gcagcgcctg gcgcaccccc
     3121 cgccggacca cagctcgtag atcggggttc ctccaagcgg gtcgggaggc aggatcacgt
     3181 tgatctcgtt cgatgttgtt tcgagatcga acgacccgtc tggggcggtg acgacatcgc
     3241 caccgtacgc cgcagcgagg cggtcgacct gttcccggtc gcgggtcgtg aacctgaacg
     3301 tgtcgatgct cgcagggcgc atccgccccg acgacgtcga cgcgagcttc ccgatcttga
     3361 tcttccccgc ctcctggggt cgcccccggg caacttcgct gatcggcctc actgggcgtg
     3421 acatgaaaat cctgatcctt tcgttagccc cactcaacgg gggagtcgtc ggagacgctg
     3481 agcgtcccgt cgacgtcgat gttcgctctg tgaacggagg cgctggcggt caactccaga
     3541 ccgtcgaacc attcgccgct gctgtcgtcg tggacgtacc tccaccggac ctcgatcctc
     3601 agcgactccg ggctggacat gacgagcggg tcagagagga accgctgcac cgccgccgaa
     3661 atccccgcag ccgtttccgc agcaggagcc tccggccgaa actggtgaac ctcggtccgg
     3721 gtgatgatct cgtcacccac ggttcacccc cttcagttcc cgtgcccgca tctccccagc
     3781 ttcggtgatc cgacagacca tcacgtcagc cccgttcggg ccggtgatcg tcccgaacgg
     3841 ctcaacgaac cctccttgac ggagttccga ggcccgcttc caccagcaca ccccaggcaa
     3901 gtccagcccg gcagcctccg cggcagcggc gtcagacagc cccgcagggg cgttccagta
     3961 ggcgacgagc atccttgccc gatgcgaacc tgggcgaacc ccgaacaccg caccttcccg
     4021 cccctcggcg tcagcgcctg cggcacgcga cgttgcaggg tcagcgggcc tggacgtctg
     4081 gccgagcggc cggtggcagc acgggcatcg ttcagtcatc gctccacctc ccgcaatacg
     4141 gagcggagcc ggtcgatcac agcgagcagg aaccgctgcg
//
LOCUS       DAOOMA010000180         4147 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_25878_length_4147_cov_3.524682, whole genome shotgun sequence.
ACCESSION   DAOOMA010000180 DAOOMA010000000
VERSION     DAOOMA010000180.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 4147)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4147)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4147
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25878_length_4147_cov_3.524682"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..554
                     /locus_tag="PLP95_06070"
     CDS             <1..554
                     /locus_tag="PLP95_06070"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HPB45402.1"
                     /translation="GLPMPTVTLGISDGNAGLAAAGATGEVLVKSPLLMAGYWNKPDA
                     TASTIRDGWLYTGDIGFIDDDGYLHITDRSKDMIIRGGENVYCVEIENCLVEHPRIAD
                     AAIIGVPHNGLGEEVKAVIEVVGGETLDADEVRAWVAERLAPFKVPTYVEFHDGKLPR
                     NASGKLLKNILRGSGEVSMAETM"
     gene            551..790
                     /locus_tag="PLP95_06075"
     CDS             551..790
                     /locus_tag="PLP95_06075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB45403.1"
                     /translation="MSDQPPLPDGSYDVFIVDAMAHPDDEQRVAGVEITITAGEHKGA
                     SFALAATGLAGSDIELIGLPATLTVVEGEPKLTLD"
     gene            complement(804..2114)
                     /locus_tag="PLP95_06080"
     CDS             complement(804..2114)
                     /locus_tag="PLP95_06080"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional o-acetylhomoserine/o-acetylserine
                     sulfhydrylase"
                     /protein_id="HPB45404.1"
                     /translation="MSESWGFETRQIHAGQEPDPTTGATVVPIYQTTSYQFRDSDHAA
                     NLFALSEIGNIYTRLMNPTQGVLEARLASLEGALTTAVGLPGALAVASGQSAATLAIL
                     NIVEAGEHLVASPSLYGGTYNLLHHTLPKLGIEVTFVEDPHDLDQWRDATKENTRAYF
                     GEVLANPRNDVFDIEGISTLAHELGVPLIVDNTVPSPYGINPIKWGADIVVHSTTKFI
                     GGHATSIGGAIIEGGSFDYTAHGKHRGMIDPDPSYHGLAFHPALGAGAYTVKARVQLL
                     RDLGPATSPFNAFLTLQGVETLSLRMERHFENAQTVADFLVRHPQVRSVNYSGLDSSP
                     WHKRAEKYFEGRGFGSVLAFEIEGGREAGRSFVDSLRLHSHVANIGDVRSLAIHPAST
                     THSQLTPEEQAASSVTPGLIRLSVGLETIDDILADLEVGFAAAK"
     gene            complement(2237..3052)
                     /locus_tag="PLP95_06085"
     CDS             complement(2237..3052)
                     /locus_tag="PLP95_06085"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: protein motif:HMM:NF020133.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004413 - homoserine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HPB45405.1"
                     /translation="MRVSVPASSANLGPGFDSLGLALTLPFIASTEPGESLLQADDRH
                     PLVVAYQRAGGSRPLWWRSPIPPGRGLGFSGAARVAGALLGVEETGDAESEAQVRARA
                     LELAIELEGHPDNAAASMLGGLTAAVGTTAVRVPLGAALDVVVWWPTTVTSTRKARAA
                     LPEMVPFVDAVFNVAHASVLVAALAAGDLDAIGEAMHDRLHQDLRLAMSPPSQEALDA
                     MRAAGVVAAWLGGSGPTVACFVEPGDGLRVVAKLPTSGVARVLQIDDRGARFT"
     gene            3263..3637
                     /locus_tag="PLP95_06090"
     CDS             3263..3637
                     /locus_tag="PLP95_06090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB45406.1"
                     /translation="MSEGADLLDAGDGCLPDLGEGIAIALLVIAAALLFIFLGIPLLI
                     ALGELALLLVLFVIGVVTKVVFRRPWTVEAIDHTGERHAWRVVGWRASGEVRNLVAER
                     VTTTGAVPTIAEVSMMAGVVAE"
     gene            complement(3672..4106)
                     /locus_tag="PLP95_06095"
     CDS             complement(3672..4106)
                     /locus_tag="PLP95_06095"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: protein motif:HMM:NF003588.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HPB45407.1"
                     /translation="MTDGVKPPPSLVNIYKELHSDLGCPIPSHGNLEPWARQGVLLLN
                     AVLTVRAHKAGSHANKGWERFTDRVISAVSDKPERVVFILWGAYARKKKPLIDTSRHV
                     VIESAHPSPLSAHNGFIGSRPFSRANEALAEAGRDPIEWCLG"
BASE COUNT          710 a         1352 c         1395 g          690 t
ORIGIN      
        1 tcgggctgcc gatgccgacg gtgaccctcg gaatctccga cggaaacgca ggcctcgccg
       61 ctgccggggc cacgggcgag gtgctcgtca agagtcccct gttgatggcc gggtactgga
      121 acaagcccga tgccaccgcc tccaccatcc gcgacggatg gctctacacc ggcgacatcg
      181 gcttcatcga tgacgacggc tacctgcaca tcaccgaccg ctcgaaggac atgatcatcc
      241 gcggcggcga aaacgtctac tgcgtcgaga tcgagaactg cctcgtcgaa caccccagaa
      301 ttgccgatgc cgccatcatc ggcgtgcccc acaacggcct cggcgaggag gtcaaggccg
      361 tgatcgaggt cgtgggcggt gagaccctcg atgccgacga ggtgcgtgcc tgggtcgccg
      421 agcgcctcgc tccgttcaag gttccgacct acgtcgagtt ccacgacggc aagctgcccc
      481 ggaatgcgtc gggcaagctg ttgaagaaca tcctgcgagg cagcggcgag gtctcgatgg
      541 cggagacgat gtgagcgatc aaccgccgct gccggatggc agctacgacg tgttcatcgt
      601 cgacgccatg gcgcaccccg acgacgaaca gcgggtcgcc ggggtggaaa tcaccatcac
      661 cgccggcgaa cacaagggtg cgtcattcgc cctcgccgcg accggcttag ccggcagcga
      721 catcgaactg atcggcctgc cggccacgct caccgtcgtc gagggtgaac cgaagctcac
      781 cctcgactga cggcgctgta ctgctacttc gccgcggcga agcccacctc gaggtcggcg
      841 aggatgtcgt cgatggtttc gaggccgacc gacaggcgga tgagccccgg ggtcaccgac
      901 gaggccgctt gttcctcggg ggtgagctgg ctgtgggtgg tcgaggccgg gtgaatcgcc
      961 aacgaacgaa catcgccgat gttggcgacg tggctgtgca gcctgagcga gtcgacgaag
     1021 ctgcgcccgg cctcgcgacc gccctcgatc tcgaacgcga gcaccgagcc gaagccgcgc
     1081 ccctcgaagt acttctcggc acgcttgtgc cacggtgagg agtcgagccc gctgtagttc
     1141 acgctgcgga cctgcgggtg ccgaacgagg aagtccgcca ccgtctgggc gttctcgaaa
     1201 tgacgctcca tgcggaggct gagcgtctcg accccctgca gcgtcaagaa cgcgttgaac
     1261 ggcgaggtgg cggggcccag gtctcgaagc agttgcacgc gagccttgac cgtgtacgca
     1321 ccggcgccga gcgccgggtg gaaggcgagg ccgtgatagg acggatcggg gtcgatcatg
     1381 ccgcggtgtt tgccgtgtgc ggtgtagtcg aagcttccgc cctcgatgat ggcgccaccg
     1441 atcgaggtgg cgtggccgcc gatgaacttg gtggtcgagt gaacgacgat gtcggcgccc
     1501 cacttgatcg ggttgatgcc atagggcgac ggcacggtgt tgtcgacgat gagcggaacc
     1561 ccgagttcat gggcgagcgt ggagatgccc tcgatgtcga acacgtcgtt tcgagggttg
     1621 gccagcacct caccgaagta cgcccgggtg ttctccttgg tggcgtcgcg ccattgatcg
     1681 aggtcgtggg gatcctcgac gaaggtcacc tcgatgccga gcttgggcag ggtgtgatgc
     1741 aacaggttgt aggttccgcc gtacagcgaa ggcgacgcca cgaggtgctc ccccgcctcc
     1801 acgatgttga ggatggccag cgtcgcagcc gactgccccg aggccaccgc aagcgcgccg
     1861 ggcagcccga ccgccgtggt gagcgcccct tcgagcgagg cgagtcgggc ctcgagaacg
     1921 ccctgggtgg ggttcatgag acgggtgtag atgttgccga tctcgctgag cgcgaaaagg
     1981 ttggccgcat gatcggagtc acggaactga taggaggtcg tctgatagat cggcacgacc
     2041 gtcgcgccag tggtcggatc cggctcctga ccggcgtgga tctgccgggt ctcgaaaccc
     2101 catgactcgc tcatttgcac tcctcgttga gtaggtcttc gttgagtagg tctgtcgttg
     2161 aatagaccgc tgtgacgagc accctaggtt cccaattccc gaccaaacaa gtcgggttta
     2221 gctcgggcag gtgccctcac gtgaaccgtg ctccgcggtc atcgatctgc agcaccctcg
     2281 cgacccctga tgtcggcaac ttcgccacca cccgcaagcc gtcgcccggt tcgacaaaac
     2341 acgcgacggt cggcccagaa ccgccgagcc aggccgcgac aaccccagcg gctcgcatcg
     2401 cgtccagcgc ctcctgtgat ggcggcgaca tggcgaggcg gaggtcctgg tggagacggt
     2461 cgtgcatcgc ttcgccgatc gcatcgaggt cgccggctgc gagcgcagcg acgagcaccg
     2521 acgcgtgggc gacgttgaac acggcgtcga caaagggcac catctcgggc agcgccgctc
     2581 gcgcctttcg ggtggaggtc accgtcgtcg gccaccacac cacgacgtcg agcgcagcgc
     2641 cgagcggcac ccgaaccgcg gtcgtgccca ccgccgcggt gaggccgccg agcatcgagg
     2701 ccgccgcgtt gtcggggtgt ccttcgagtt cgatcgcgag ctccaaggct cgagcgcgga
     2761 cctgcgcctc ggattctgca tcgccggttt cctccacccc gagcagggca cccgccacgc
     2821 gtgctgcacc ggaaaagccg aggccgcgcc ctggagggat cggcgagcgc caccacagcg
     2881 gcctcgaacc gccggcgcgc tgatacgcga ccaccagcgg atggcggtcg tctgcctgaa
     2941 gcaacgactc acccggctcg gtcgacgcga tgaacggcag agtcagcgcg agcccgaggc
     3001 tgtcgaaacc ggggccgagg ttggcggacg acgccgggac gctcactcgc atggctccac
     3061 gctaccggtc tcttccgaca tcgattctcg atatctcctg gcacactggc ggacgtgaga
     3121 atcgtggagc acgggtgatg gcgtgaaggt cgaaaccccc ttgggaacct gggtggttcg
     3181 tcgtcggtgg gtgccgcgac agctcggaaa cgacagctac tggtcccgct ttcggagccg
     3241 gaactccaag gcgcggcgcc gaatgagcga gggcgcggac ctactcgatg ccggcgacgg
     3301 gtgccttccc gacctcggcg aggggatcgc catcgcgctc ctcgtgatcg ccgcggcgtt
     3361 gttgttcatc ttcctcggga tcccgctgct gattgcgctc ggcgaactcg ccttgctgct
     3421 cgtcctgttc gtgatcggcg tcgtcaccaa ggtcgtgttt cgacgcccct ggaccgtcga
     3481 ggcgatcgat cacacgggtg aacgtcacgc ctggcgggtg gtgggctggc gggcgagcgg
     3541 cgaggtgcgc aacctcgtcg ccgagcgggt cactacgacc ggggccgtcc cgacgatcgc
     3601 tgaggtgtcg atgatggccg gggtcgtcgc cgagtgaagg cggacctatc gcgactcagc
     3661 ccaggtgaca ttcagcccag gcaccactcg atgggatctc gtcccgcctc ggcgagtgcc
     3721 tcgttcgctc gactgaacgg tcggctcccg atgaacccgt tatgggccga gagcggagac
     3781 ggatgggccg attcgatgac gacgtgtcgc gacgtatcga tcagcggctt ctttttacgc
     3841 gcataggcgc cccacaagat gaacaccacc cgctctggtt tgtccgacac cgctgagatc
     3901 acgcggtcgg tgaagcgttc ccaccccttg ttcgcgtgcg agccggcctt gtgcgcccgc
     3961 accgtcaaca ccgcgttcaa caacagcacc ccctggcgag cccacggctc gaggttgccg
     4021 tgggatggaa tcggacagcc cagatcgctg tggagctcct tgtagatgtt caccaacgat
     4081 ggcggaggct tcacgccgtc ggtcaccgag aagctgccct tcaacgacgg ccatcggcac
     4141 tatggca
//
LOCUS       DAOOMA010000181         4106 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_26228_length_4106_cov_3.377685, whole genome shotgun sequence.
ACCESSION   DAOOMA010000181 DAOOMA010000000
VERSION     DAOOMA010000181.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 4106)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4106)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4106
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26228_length_4106_cov_3.377685"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..422
                     /locus_tag="PLP95_06100"
     CDS             <1..422
                     /locus_tag="PLP95_06100"
                     /inference="COORDINATES: protein motif:HMM:NF024591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB45408.1"
                     /translation="LDSAGELHYDGRATEMIKSGGANISPAEIEVALRACAGVKLARV
                     IGVDDERLDQIAIACVEVADGAPLTAHDVTSFLRERIATYKVPRRVLIFDAGEIPLTS
                     SGTKVRDAELRSLIAERLAAERLAGEPAPTEPAPTER"
     gene            434..1639
                     /locus_tag="PLP95_06105"
     CDS             434..1639
                     /locus_tag="PLP95_06105"
                     /inference="COORDINATES: protein motif:HMM:NF024844.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase N-terminal
                     domain-containing protein"
                     /protein_id="HPB45409.1"
                     /translation="MTSQDQTSQDQPTLDTSDLDRHMGVPMEPGELKEPVALNDIRRW
                     VQAMHYPNPLHYDERWSAESRFGTIVAPQSMTVATDTSHGCSPAQVGKIPNSHLIFGG
                     DDWWFFGPRIWPGDHMVCHRMPYDYKVSDTKFAGPTCFQRGDTLYINQRGERVALQRS
                     TAIRYQVREAKEKKLFSEPKEPEWSDAKLAELDEIKGWFIDQIQTLRHDPRTYESVSV
                     GDMLAPNVLGPHSLASFTTEWRAFPMTTWGATAKGPTTVRAEELGYTKEMAGFEGDRR
                     MERTNPELTDGAYYGPSRGHLQPRWAQHVGMPRGYGYGASMGAWIIDYVAAWAGEWGF
                     VTHATSQYRNPAFTGDATFLSGEVIGKEILRRGRHRVKVAVELRNQDETVMAKATVEV
                     ELPSTDTLS"
     gene            1723..2040
                     /locus_tag="PLP95_06110"
     CDS             1723..2040
                     /locus_tag="PLP95_06110"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative quinol monooxygenase"
                     /protein_id="HPB45410.1"
                     /translation="MILVVVKQPVRAKYADDWPELVREYTEATRAEPGNISFDWYRSP
                     EDPNLWILVEVFADKAAGDAHTASEHFAKASGEMARWCAAIPEIIHMEGPGEGWSLAG
                     EAS"
     gene            2089..2856
                     /locus_tag="PLP95_06115"
     CDS             2089..2856
                     /locus_tag="PLP95_06115"
                     /inference="COORDINATES: protein motif:HMM:NF004855.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HPB45411.1"
                     /translation="MTSDRQQPTFPTFDTVEEERRHRKQRLAAAFRLFGAFGFDEGAA
                     GHITARDPELTDHFWVNPLAMNFKRIRVRDLLLVNAAGEVVHGTWRVNKAAFMIHSHI
                     HEARPDVVAAAHSHSIYGKTLSTLRQPLLPLTQDACAFYGDHAVFDDYGGVVFEHDEG
                     ERIAEALGSHKAAILANHGLLTVGATVDEAAWWFITMERSCQAQLLAEAAGDPVPIPH
                     EQAAATASMIGHPMSGWYSFQPLYDWIVTEQPDFLEE"
     gene            2860..>4106
                     /locus_tag="PLP95_06120"
     CDS             2860..>4106
                     /locus_tag="PLP95_06120"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HPB45412.1"
                     /translation="MAVSWSIARVLDDVLATDPGREALVTRSARLSYADLDAAANRAA
                     QALVSCGVRPGDRVAASLPNDVDVVVAFHGAMRAGAVWLGINRALAVPEKQFQLDDTE
                     AAVFICENAGGAGAGAGAGAGDVVGAWAVGGTTLSLGEWQSLVAGASDDAVGIEVDPL
                     APAGIAYTSGTTGRPKGVVHSQRGLLVPATVLVESRGYDSTLRKADCFPFTILNMTVL
                     TTLLAARAGGTTILMDRIDAEGVAEWIRDEAATLWNGPPALLHSLASMDSVAASDLGS
                     LREAWTGGADCPATILDAYEAKFGHRVLSTYGLTEAPTVVSIDPIEAAALDGKVGRVE
                     GASGRPLPHLAVTIRDEAGDEVAAGETGEVCVGPAAGDDRYRLMLEYWRRPEATAETL
                     GGGALHTGDVGFFDEAGYLHLRDR"
BASE COUNT          685 a         1311 c         1450 g          660 t
ORIGIN      
        1 acctcgactc cgccggtgag ctgcactacg acggccgtgc cacggagatg atcaagtccg
       61 gcggcgccaa catctccccg gccgaaatcg aggtggcgtt gcgggcgtgc gcgggggtga
      121 aactcgcccg cgtgatcggc gtcgacgacg aacgtctcga ccagatcgcc atcgcctgtg
      181 tcgaggtggc cgacggcgcc ccgctcaccg cgcacgacgt cacctcgttc cttcgtgaac
      241 ggatcgccac ctacaaggtt ccgcggcggg tgctgatctt cgacgccggc gagatccccc
      301 tcacctcgag tggcaccaag gtccgcgacg ccgagttgcg ctcactcatc gccgaacgcc
      361 tcgccgccga acgcctcgcc ggcgagccag caccgaccga acccgcaccg accgaacgtt
      421 gaatggagac ctcatgacat cacaggacca gacctcacag gaccagccga ccctcgacac
      481 cagcgatctc gaccgccaca tgggcgtgcc gatggagccg ggcgagttga aggagccggt
      541 cgcgctcaac gacatccggc ggtgggtgca ggcgatgcac tacccgaacc cgctgcacta
      601 cgacgagcgg tggtcggccg aaagccgttt cggaaccatc gtcgctccgc agtctatgac
      661 ggtggccacc gacaccagtc acgggtgttc tcccgcgcag gtcggcaaga ttcccaatag
      721 tcacctgatc ttcggtggcg acgactggtg gttcttcggg ccgcggatct ggccgggcga
      781 tcacatggtc tgtcaccgga tgccgtacga ctacaaggtg tccgacacca agttcgccgg
      841 gccgacgtgt ttccagcgcg gcgacaccct ctacatcaac cagcgcggcg aacgggtggc
      901 gcttcagcgt tcgacggcga ttcgctatca ggtgcgtgag gcgaaggaga agaagctgtt
      961 ctccgagccg aaggaacccg aatggagcga tgcgaaactc gcggagctcg acgagatcaa
     1021 gggttggttc atcgatcaga tccagacgct gcgccacgac ccgcgcacct atgagtcggt
     1081 gagcgtcggc gacatgctcg ccccgaacgt gttgggcccc cattccctcg ccagtttcac
     1141 caccgagtgg cgggcgtttc ccatgaccac ctggggagcg acggcgaagg ggccgaccac
     1201 ggtgcgcgcc gaggagctcg gttacaccaa ggagatggcg ggctttgaag gcgaccggcg
     1261 aatggaacgc accaaccccg aactcaccga cggcgcctac tacggaccgt cgcgggggca
     1321 cctgcagcct cgctgggctc aacacgtcgg catgccgcgt ggctacggct acggggcgtc
     1381 gatgggtgcg tggatcatcg actatgtggc ggcgtgggcc ggggagtggg gctttgtcac
     1441 ccacgccacc tcgcagtacc gcaacccggc cttcaccggc gacgccacgt tcctgtcggg
     1501 tgaggtgatc ggcaaggaga tcctgcgccg aggccgtcac cgcgtgaagg tcgctgtcga
     1561 gttgcgcaac caggacgaga cggtgatggc caaggccacc gtcgaggtcg agttgccaag
     1621 caccgacaca ctgtcctgag cagcactttc ctgagcagcg cccgtcaagg ccgtgcccgt
     1681 caatgccttg cccgtcaaac cccaccgaac caggagcgaa tcatgattct cgtcgtcgtc
     1741 aaacaacccg tccgtgccaa gtacgcggac gactggcccg aactcgtgcg cgagtacacc
     1801 gaggccaccc gcgccgagcc gggcaacatc tctttcgact ggtatcgcag cccggaggat
     1861 ccgaacctgt ggatcctcgt cgaggtgttc gccgacaagg ccgccggcga cgcgcacacc
     1921 gcgtcggagc acttcgccaa ggcgagtggc gagatggccc gctggtgcgc cgccatcccc
     1981 gagatcatcc acatggaggg gccgggggag ggctggtcgc tcgccggcga ggcctcctag
     2041 cgccgtggca cgttcgacga tgagaccatg tccgagggag gtgcgcccat gaccagcgac
     2101 cgccaacagc cgacgtttcc gaccttcgac acggtcgagg aggagcggcg tcatcgcaag
     2161 caacgtctcg ccgcggcgtt tcgtctcttc ggggcctttg ggttcgacga gggggcggcg
     2221 ggccacatca ccgcccgcga tccggagttg accgatcact tctgggttaa cccgctggcg
     2281 atgaacttca agcgcatccg ggtgcgcgac ctgttgttgg tgaacgccgc gggcgaggtc
     2341 gtgcacggca cctggcgggt gaacaaggcg gcgttcatga tccactcgca cattcacgag
     2401 gcgcgccccg acgtggtggc ggcggcgcat tcgcattcga tctacggcaa gacgctgtcg
     2461 acccttcgcc agccgctgct gccactcacc caggatgcgt gcgcgttcta cggcgaccat
     2521 gcggtgttcg acgactacgg gggtgtggtg ttcgaacacg acgagggcga gcgcatcgcc
     2581 gaggccctgg gttcgcacaa ggcggcgatc ctcgcgaacc acgggctgtt gaccgtcggg
     2641 gcgaccgtgg acgaggcggc gtggtggttc atcacgatgg aacggtcgtg tcaggcccaa
     2701 ctgctcgcag aggccgccgg agatccggtg ccgattcccc acgagcaggc ggcggccaca
     2761 gcgtcgatga tcggacatcc gatgtcgggg tggtattcgt tccagccgct ctatgactgg
     2821 atcgtgaccg aacaacccga cttcttggag gagtaggcga tggctgtttc gtggtcgatc
     2881 gcacgggtgc tcgacgacgt gctcgcgacc gacccgggcc gcgaggcatt ggtgacgcgg
     2941 tcggctcgac tgagttacgc cgacttggac gccgccgcga atcgtgccgc ccaggcgctg
     3001 gtgtcgtgtg gggtgcgccc cggcgaccgg gtggcggcgt cgctgccgaa cgacgtcgat
     3061 gtggtggtgg ccttccacgg tgcgatgcgg gccggggccg tgtggttggg catcaaccgt
     3121 gccctcgctg ttccagaaaa acagttccaa ctcgacgaca ccgaggcggc ggtgttcatc
     3181 tgcgagaacg ccggaggcgc tggcgctggt gctggtgctg gcgctggcga tgttgtcggg
     3241 gcctgggccg tcggcgggac gacgctgtcg ctcggcgagt ggcaatcgct cgtggcgggg
     3301 gcgtcggatg acgctgttgg gatcgaggtc gacccgctcg cgccggcggg catcgcctac
     3361 acgagtggga cgacggggcg gccgaagggt gtggtgcaca gccagcgggg gctgctcgtg
     3421 ccggcgacgg tgctggtgga gagccggggg tacgactcga cgctgcgcaa ggccgactgt
     3481 ttcccgttca cgatcctcaa catgaccgtg ctgaccacgc tgttggcggc gagggccggc
     3541 ggcacgacga ttctcatgga tcgcatcgat gccgaagggg tggccgagtg gatccgcgac
     3601 gaggcggcca cgttgtggaa tgggccgccc gcgttgttgc attcgctggc ctcgatggac
     3661 tcggtagcgg cgagcgattt ggggtcgttg cgcgaggcct ggaccggggg agcggactgt
     3721 ccagcgacga tcctcgacgc gtacgaagcg aagttcggcc atcgggtgtt gagcacctat
     3781 gggctgaccg aggcgccgac cgtggtgtcc atcgacccga tcgaggccgc ggcgctcgat
     3841 ggcaaggtcg gccgggttga gggggcgagc ggtcggccgt tgccgcatct ggcggtgacg
     3901 atccgagatg aggccggcga tgaggtcgcg gcgggggaga ccggtgaggt gtgcgtcggg
     3961 ccagcggcgg gggacgatcg ctaccggctg atgctcgagt actggcgccg gcccgaggcc
     4021 accgcggaga cgttgggggg cggggcccta cacaccggtg acgtcgggtt cttcgacgag
     4081 gcgggctatc tccacctgcg cgaccg
//
LOCUS       DAOOMA010000182         4102 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_26267_length_4102_cov_3.707438, whole genome shotgun sequence.
ACCESSION   DAOOMA010000182 DAOOMA010000000
VERSION     DAOOMA010000182.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 4102)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4102)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4102
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26267_length_4102_cov_3.707438"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            102..569
                     /locus_tag="PLP95_06125"
     CDS             102..569
                     /locus_tag="PLP95_06125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB45413.1"
                     /translation="MTTIDDVLMELSDAGEDVLVVSKARRLPLERWRNRASALPDSGL
                     VHTVVTNRQVVFTPRHKFTSDLGSAAPITRPIDEVTRAFRPHPKLRNETLCVEFADSS
                     TARFELPSREQADLWLHGLSVALRDLHRWDALRRDDRSLFPNDGDPATPPGRT"
     gene            613..1428
                     /locus_tag="PLP95_06130"
     CDS             613..1428
                     /locus_tag="PLP95_06130"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024852.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HPB45414.1"
                     /translation="MTLPAPSATSTAIVTGASSGIGVELARELAGRGHGVTLVARRED
                     RLRSLATDLTDRHGIRAEVVAADLTDPDSRSAILTTLADRGLAVDILVNNAGLSTVGA
                     VAECDPDRELAMVRTDVEAVVHLCSLTIPDMVRRGRGAVLNVASTAAFQPIPGQAGYA
                     ASKAFVLSYSQAVGEELVGSGVTVTALCPGPVATEFGDTAGFDPDDVDSSLPKFMWVP
                     ASDVARQAVAALDAGRGVVIPGLPNRISAHTAAITPRRVLLPILRRRHPGLKG"
     gene            complement(1524..2981)
                     /locus_tag="PLP95_06135"
     CDS             complement(1524..2981)
                     /locus_tag="PLP95_06135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012224073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HPB45415.1"
                     /translation="MTTNAQIRIGGDLVDGELGSYEIINPATEEVVGLAPQASVQQNL
                     DATAAAAEAFGSWSQTKPEYRAELLNKAADLWDAKSKELVPIVQAETGATVRTVKMMQ
                     YSQVSARLRRYARAALDPTITPIEPVIMPTTPLAPGGIISAAVNRAPVGVVSCLASYN
                     VPGTNMAGKIAPALAMGNTVVMKPAPQDPLGVFALAETLIEAGFPPGVVNVTTGTDIA
                     ASEALVSSRHVDMVSFTGSTGVGRRIGEVAGGDMKRLLLELGGKGACIVYDDADLKAA
                     IGGIASVWGFHSGQICTSPTRVIVQRGVYEKLVGGLTAAAQHMKVGDPLDKETVVGPV
                     ITAAHRERVMGYIQSARDEGATIAVGGDSPQVETGFYVNPTLITDATAQMTAVREEIF
                     GPVVAVVPFDDDDEAIAIANDSDFGLYSYIFSSDTGRAFNVAKRLRSGNVGINSLQRN
                     HEAPFGGFKQSGVGRDGGVYGMYAYSEMQSIVWPG"
     gene            complement(3094..3429)
                     /locus_tag="PLP95_06140"
     CDS             complement(3094..3429)
                     /locus_tag="PLP95_06140"
                     /inference="COORDINATES: protein motif:HMM:NF039183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5658 family protein"
                     /protein_id="HPB45416.1"
                     /translation="MPTPPKTAAVISVRRWLLAAGLMLLNLIDVVSTKVVLSLGGTEA
                     NPVMSDVMNHPTYPWLTKLAVCLAVTVLLFAAPAASRLAERAVTAVLLFYVAVVAWNI
                     SLIVNFYRG"
     gene            3537..3959
                     /locus_tag="PLP95_06145"
     CDS             3537..3959
                     /locus_tag="PLP95_06145"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HPB45417.1"
                     /translation="MIKRNDRLAAFRSIALFRACSDEELGQIDAVSDEVRIEAGRDLI
                     RQGDLGREFIVLVDRAVTVSRDGEEVATLGPGAHFGELALLVDQPRNATVTAITPVTA
                     QVIDRRGFQSMLDGSPHLTKNLLVSLARRLSEIEEQQH"
BASE COUNT          707 a         1432 c         1277 g          686 t
ORIGIN      
        1 ggggcgacgc ctaatgactc cacgctattg cggcgcgatc gacaacccgt gcgtgagcat
       61 ctcgccctac aggttttggg ccgtccgacc gaagaatcgc tatgacaacg attgacgacg
      121 tgctcatgga attgagcgat gcgggcgagg acgtcctcgt cgtgtcgaag gcgcgtcgac
      181 tccctctcga gcgatggcgc aatcgagcat ccgccctacc cgacagcgga ctcgtacaca
      241 cggtcgtaac gaaccgtcag gtcgtgttca cgccgcgcca taagttcacc agcgatctcg
      301 gatccgccgc gccgatcacc cgaccgatcg acgaggtgac ccgcgccttt cggccgcacc
      361 cgaagctgcg caatgaaacc ctctgtgtgg aattcgccga ttcgtcgacc gcccgttttg
      421 agttgccgag ccgtgaacag gcggacctgt ggctccatgg cctcagcgtc gcccttcggg
      481 acctccaccg ttgggatgcc ctccgccgcg acgaccgatc gttgtttccg aatgacggcg
      541 accctgcgac ccctcccggc agaacctgaa tcgacccgtc gtcgacagcg ggtagggcgt
      601 agggtcgcgc ctatgacgct gccagcacct agtgccactt cgaccgcgat cgtcaccggg
      661 gcatcgagcg gcatcggtgt cgagctcgcc cgtgagctcg caggtcgcgg ccacggggtc
      721 accctcgtgg cccggcgcga ggatcgcctg cgctccctcg ccaccgacct caccgaccga
      781 cacggcatcc gcgccgaggt ggtcgccgcg gatctcaccg atccggattc acggtcggca
      841 atcctgacca ccctcgccga ccgtgggctc gccgtcgaca ttctcgtgaa caacgcgggg
      901 ctctccacgg tgggtgccgt ggcggagtgc gaccccgacc gcgagttggc gatggttcgc
      961 accgacgtcg aggcggtcgt gcatctgtgc agcctcacca tccccgacat ggtccgccgc
     1021 ggccggggcg cggtgctcaa cgtcgcttcc acggccgcgt ttcagccgat ccccgggcaa
     1081 gccggctacg cggcgagcaa ggcgttcgtg ttgtcgtact cccaggcggt gggcgaagag
     1141 ctcgtcggct caggcgtcac ggtcacggca ctgtgtccgg ggccggtcgc caccgagttc
     1201 ggcgacaccg ccggtttcga ccccgacgac gtcgactcgt cgttgccgaa gttcatgtgg
     1261 gtgccagcca gcgatgttgc ccgtcaggcg gtcgccgcgc tcgatgccgg ccgtggcgtc
     1321 gtcattcccg gcctccccaa ccgcatcagt gcgcacaccg ccgcgatcac cccgcgccgc
     1381 gtcttgctgc cgatcctgcg ccgccgacac ccgggcctca agggttgaga caccaacggg
     1441 atctgctccc cgcctgacac tcaccgctac gacgtacggg cggcgacggc agcgaacgcc
     1501 gcgacgccgc ctcgaacgcg acctcagccc ggccagacga tcgactgcat ctccgagtag
     1561 gcgtacatgc cgtagacacc gccgtcgcga ccgacaccgg actgcttgaa accgccgaag
     1621 ggcgcctcat ggttgcgctg cagcgagttg atgccgacgt tgcccgagcg caagcgcttg
     1681 gcgacgttga acgcccggcc cgtgtcggag ctgaagatgt agctgtagag cccgaagtcc
     1741 gaatcgttgg caatggcgat cgcctcgtcg tcgtcgtcga agggtacgac ggccacgacg
     1801 gggccgaaga tctcctcgcg aaccgccgtc atctgcgcgg tggcgtcggt gatgagcgtc
     1861 gggttgacgt agaacccggt ctcgacctgg ggtgagtcgc cgccgacggc gatcgtcgca
     1921 ccttcgtcgc gcgccgattg gatgtagccc atcacccgct cgcgatgcgc tgcggtgatg
     1981 accggtccga cgacggtctc cttgtcgagc gggtcgccca ccttcatgtg ctgggctgcc
     2041 gcggtcagcc cgccgaccag cttctcgtac acgccgcgtt ggacgatcac ccgggtcggc
     2101 gaggtgcaga tctgtcccga gtggaagccc cacaccgagg caataccgcc gatggcggcc
     2161 ttgagatcgg cgtcgtcata gacgatgcac gcacccttac cgccgagttc caacaacagg
     2221 cgcttcatgt cgccgcctgc aacctcgccg atgcgacgcc ccacgcccgt cgacccggtg
     2281 aaactcacca tgtcgacgtg ccgagagctc acgagtgctt cggaggcggc gatatcggtt
     2341 ccggtcgtga cgttgacgac gcccggggga aagcccgcct cgatgagtgt ttcggcgagc
     2401 gcgaacaccc cgagcgggtc ctgaggtgcc ggcttcatga cgacggtgtt tcccatcgcc
     2461 agcgccggag cgatcttgcc ggccatgttc gtgcccggaa cgttgtacga ggcgagacaa
     2521 ctcaccacac ctaccggcgc acggttgacc gctgccgaga tgatgccgcc gggggcgagc
     2581 ggcgtggtcg gcatgatcac cggctcgatc ggggtgatgg tcgggtcgag cgcggcgcgg
     2641 gcatagcgac gcagacgagc cgacacctgg ctgtactgca tcatcttgac cgtgcgcacc
     2701 gtggcaccgg tctcggcctg cacgatcgga acgagttcct tcgacttcgc atcccacagg
     2761 tcggcggcct tgttcagcag ttcggcccgg tactccggct tcgtctgcga ccacgacccg
     2821 aacgcctcgg cggcggcggc ggtcgcgtcg aggttctgtt gcaccgacgc ctgcggggcc
     2881 aacccgacca cttcctcggt ggccggattg atgatctcgt agctgccgag ttccccgtcg
     2941 acgagatcgc cgccgattcg aatctgtgcg ttggtcgtca tctgacgcat cccccatgag
     3001 cttcacgaag tcaattgtgc gcccacggta caccgcccct gacgatgtgt cagaaacgca
     3061 acccggcacg gcggtccacg ccgtccacct cgttcaacct cggtaaaagt tgacgatcag
     3121 cgagatgttc cacgccacca ccgccacgta gaacaacagc accgcggtca cggctcgttc
     3181 ggcgagcctc gacgcggccg gtgctgcaaa caacaacacc gtgaccgcca ggcagacggc
     3241 gagcttggtg agccacgggt aggtcggatg gttcatcaca tccgacatca ccgggttcgc
     3301 ttcggtgccc ccgagcgaca ggacgacctt ggtcgacacc acatcgatga ggttcaacag
     3361 catcaacccc gcagccaaca gccaacgccg caccgagatc acggccgcgg tcttgggagg
     3421 tgttggcatt gtcgaatcgg aattggtcac gcatgtcctc cacatcgggt caccgaccga
     3481 accttcggcg cacggtaagg aacgccacgc ccgatgacaa ggggtagcct gcggtcatga
     3541 tcaaacgaaa tgaccggctg gccgcgttcc gctccatcgc gttgtttcgc gcgtgcagcg
     3601 acgaggaact cggccagatc gacgcagtat ccgacgaggt tcgcatcgag gccgggcgcg
     3661 acctcatacg ccaaggcgac ctcggccgcg aattcatcgt gttggtcgac agagcggtca
     3721 cggtgtcgcg cgacggagaa gaggtcgcca cactcggacc gggcgcgcat ttcggcgaac
     3781 tcgcgctcct cgtcgatcag ccacgaaacg ccaccgtcac cgccatcacc ccggtcacgg
     3841 cacaggtcat cgaccgtcgc ggcttccaga gcatgctcga cggctcaccg catctgacca
     3901 agaacctgct ggtgtcgttg gcccgccgcc tcagcgagat tgaagaacag cagcactgac
     3961 ctgcggccac acctcggcac cgaatcgttc gatctgttcg accatctcgg ccgcggatcg
     4021 tgcagcaaag cggacctgca actggttcgc cccgaccccg agatagcgcg tcaggcgcac
     4081 agccatctcc tcactcgacg tt
//
LOCUS       DAOOMA010000183         4095 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_26334_length_4095_cov_3.863119, whole genome shotgun sequence.
ACCESSION   DAOOMA010000183 DAOOMA010000000
VERSION     DAOOMA010000183.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 4095)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4095)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4095
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26334_length_4095_cov_3.863119"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            166..477
                     /locus_tag="PLP95_06150"
     CDS             166..477
                     /locus_tag="PLP95_06150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4258 domain-containing protein"
                     /protein_id="HPB45418.1"
                     /translation="MASAQRPKAWTFPIVITEHAYDKLIAIDLTYSEFEYLLRTSAEV
                     IEDIEVDHRSTKEVILYLHWIRPLHVVVLVDRHRREERVVTVYEPTTNRWSHDFRRRK
                     R"
     gene            complement(832..2268)
                     /locus_tag="PLP95_06155"
     CDS             complement(832..2268)
                     /locus_tag="PLP95_06155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012224014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HPB45419.1"
                     /translation="MSNYDSFYIGGQWVAPVSDNGSAEVIHSATEELIATVPLGTAAD
                     VDAAVAAAKTAFETWSTTPADIRAKYLRDISAGLAARYGDIATTIQSEVGSPKTFSEL
                     IQAGLAVSDWENYAAIIETYEWETQLGNSLIVREPIGVVGAITPWNYPLYLIVCKLGP
                     ALAAGCTVVLKPSEVTPLNAYILAEVIHEVGLPAGVFNMVIGDGPTVGSAMSKHADID
                     MISFTGSGRAGAQVTKDSADTVKRVGLELGGKSANVVLDDADPAAVAMSAVFGCFINS
                     GQTCSALTRVLIPASREQEYLDAIVAEASKVHLVDPHEEAPDLFHALGPLSSKAHQER
                     VRSYIQKGIDEGAQLVLGGPEQPEDKEKGFYVKPTVFAGVTPEMSIAQEEIFGPVLSV
                     LTYTDDDDAVRIANGTIYGLSGAVQSNDLERAKGIARRMRTGQVDINGGSFNAVAPFG
                     GYKQSGNTRERGQWGLEEFLETKSIQLP"
     gene            2550..3650
                     /locus_tag="PLP95_06160"
     CDS             2550..3650
                     /locus_tag="PLP95_06160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB45420.1"
                     /translation="MNLPDVRLHTNRQPQRTPRRARRSVVVLIAAALLVTATACDVAV
                     AGRRCAGSGFGRDATHILQCKNRKWTRIMTFGQYAELMARVQAEQERATSSAARAEVT
                     RGTSAWVDVYDWSPTWVNFKAPGSKAPFTLDRIDRMAESGITALWIQTAKVDFGSDVL
                     DRDLLVRIINRARSKGMKVFGWYLPTFENPAVDQNLLAKTAELGLDGIGVDIESTVVD
                     LATRNARLVSLSQWLRAAYPSMPLGAIVLPPVVTEVLNLNYWPQFPWTELAPFYDVWM
                     PMGYWTNRSATSEWRDAFRYTTENIDRIRTNLNNPNAPIHAIGGLSNTSSIDDVNRFV
                     FGAKLRGAYGGGLYDDMISTADQYRALAPFRG"
     gene            3843..>4095
                     /locus_tag="PLP95_06165"
     CDS             3843..>4095
                     /locus_tag="PLP95_06165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB45421.1"
                     /translation="MAVTDANRLAMHKDLTAALGEESANTLMEHLPTKGAAELATKTD
                     LDNLRTELDARFDKIDARFDKIDARFDKTGRNVYAARLCC"
BASE COUNT          757 a         1329 c         1299 g          710 t
ORIGIN      
        1 aacaggcaag gcgaggtagg gcgccgcgtc gaccaatacc tgctcgacaa ggtgagatag
       61 actcgccaag ggcagcgacg cctgcggccg caccgtatct gccaacatga tcgcctgagc
      121 cacttcgcca caggcccgta tacacggact agcgaaggaa cccagttggc aagcgcgcaa
      181 cgtccgaaag cgtggacctt tccgatcgtc atcacggagc acgcctacga caagctcatc
      241 gcgatcgacc tcacgtattc agagttcgag tacctcctgc gaacctcagc cgaagtgatc
      301 gaggacatcg aggtggatca tcgctccaca aaggaagtga tcctgtacct acactggatt
      361 cgaccgcttc acgtcgtagt cctcgttgac cgtcatcgcc gtgaagagcg ggtcgtcacc
      421 gtgtacgaac cgacgaccaa ccgatggtct cacgacttcc gaaggaggaa gcgataatgc
      481 ggtgcactgc ctgtggaagc gaagagcgtc attgtgttcg ccactccaaa ttgctggaac
      541 gtaatggggt cgtgatcgtg gtacgggacg ttcctatgga agagtgctcg cactgcgaaa
      601 cccggtggct caccgccgac accgcccgca ctctcgacca gctcttccca ctcctgcggt
      661 ccgctcagcc gggatcgacc atcgttcggg agtgggacga tctcaccccg accgctgcgt
      721 aacgcgctac cgccccactt ctgagcccac cccagcccgt ttcccgataa ccagtggtcg
      781 tgatcacgac cactggttat cggagaacag gcaaggacag gcaggacctg gttacggaag
      841 ctggatcgac tttgtctcga ggaattcctc gaggccccac tggccacgct cgcgggtgtt
      901 gcccgactgc ttgtagccgc cgaagggcgc cacggcgttg aacgatccgc cgttgatgtc
      961 gacctggccg gtgcgcatcc ggcgggcgat tcccttggca cgctcgaggt cgttcgattg
     1021 caccgctccc gacaggccat agatcgtgcc gttggcgatg cgcacggcgt cgtcgtcatc
     1081 ggtgtaggtg agcaccgaca gcaccgggcc gaagatctcc tcctgtgcga tcgacatctc
     1141 gggggtgacg ccggcgaaca cggtcggctt cacgtagaaa cccttctcct tgtcctcggg
     1201 ttgttcgggt ccgcccagca ccaactgtgc gccctcgtcg atgccctttt gaatgtacga
     1261 acgcacccgc tcctgatggg ccttcgagct gagcgggccg agggcgtgga acaggtccgg
     1321 agcttcttcg tgcgggtcga cgaggtgcac cttcgacgcc tccgccacga tcgcgtcgag
     1381 gtattcctgc tcgcggctcg ccgggatcag cacgcgggtg agcgccgaac acgtctggcc
     1441 ggagttgatg aagcagccga acacggccga catggcgaca gccgcggggt cggcatcatc
     1501 gaggaccacg ttggccgact tgccgccgag ttcaagcccg acgcgcttga cggtgtcggc
     1561 agaatccttg gtgacctgcg cgcccgcacg gcccgagccg gtgaagctga tcatgtcgat
     1621 gtccgcgtgc ttcgacatcg ccgatccgac cgtgggtccg tcgccgatga ccatgttgaa
     1681 cacgcctgcg ggaaggccga cctcgtggat gacctcagcg aggatatagg cgttgagcgg
     1741 cgtgacttcg gagggcttca acacgacggt gcagccggcg gcgagggccg ggccgagctt
     1801 gcacacgatg agatacagcg ggtagttcca cggcgtgatc gcgccgacga cgccgatcgg
     1861 ctcgcgcacg atgaggctgt tgccgagttg ggtttcccac tcgtaggtct cgatgattgc
     1921 ggcgtagttc tcccaatccg acacggcgag gccagcctgg atgagttcgg agaacgtctt
     1981 gggcgatccg acctcggact ggatggtggt ggcgatgtcg ccgtagcggg cggcgagacc
     2041 ggcgctgatg tcgcgcaggt acttggcgcg gatgtcggcg ggcgtggtcg accaggtttc
     2101 gaacgccgtt ttggccgcgg cgacggcggc gtcgacatcg gctgcggtgc cgagcggcac
     2161 ggtggcgatc agttcctcgg tggcggagtg aatgacctca gcgctgccgt tgtcactcac
     2221 cggagcgacc cactggccgc cgatgtagaa cgagtcgtaa ttactcatgg tgtccccttg
     2281 tcccggtgtt gtgcgttccc ctgagtcgcc gcgggatcgg cgctctgagc tcacgctacc
     2341 gccccgacgg ggcgccgcgg acgcgaaccg ggtggggcgg gtgcgccgcc atcccgccga
     2401 ctcgccgccg cctcgcgggc caccatcgcc gtcggcccgc gaactttgtg ctgaaagcga
     2461 catgacgtgt cactttcagc acaaagttcg cggacagagc cagccgaccg cgccagccag
     2521 cgacagcggc gtgggtacgc tcgcgcacca tgaatctccc cgacgtccgc ctccacacga
     2581 accgtcagcc ccagcgaact ccccgccgcg cacgtcggtc ggtggtcgtg ttgatcgccg
     2641 ccgcgttgct ggtgaccgca accgcgtgtg acgtggccgt cgcgggtcgg cgctgcgccg
     2701 gcagcgggtt cggccgcgac gccacccaca tcctgcaatg caagaaccgt aagtggaccc
     2761 gcatcatgac cttcggacag tacgcggagc tgatggcgag ggtgcaggcc gaacaggaac
     2821 gtgcgacctc gagcgcggcc cgagccgagg tcacccgggg taccagcgcg tgggttgacg
     2881 tctacgactg gtcaccgacg tgggtgaact tcaaggctcc cggctccaag gctcccttca
     2941 ccctcgatcg aatcgatcga atggccgagt ccggcatcac ggcgttgtgg atccagaccg
     3001 cgaaggtcga cttcggcagc gacgtgctcg atcgtgatct gctggtgcgc atcatcaacc
     3061 gagcacgcag caaggggatg aaggtgttcg gctggtatct gccgaccttc gagaatcccg
     3121 ccgtcgacca gaacctgctc gccaagaccg ccgagttggg cctcgacggc atcggggtgg
     3181 atatcgagtc gaccgtggtc gatctcgcca cccgcaacgc acgcctggtg tcgctgtcgc
     3241 agtggctgcg ggcggcgtat ccgtcgatgc cgctgggtgc gatcgtgttg ccgcccgtgg
     3301 tgaccgaggt gctcaacctc aactactggc cgcagttccc gtggaccgaa ctggcgccgt
     3361 tctatgacgt gtggatgccg atggggtatt ggacgaaccg cagcgcgacc tcggagtggc
     3421 gagacgcgtt ccgctacacg accgaaaaca tcgatcgcat ccgaaccaac ctcaacaacc
     3481 cgaacgcgcc gattcacgcc atcggtggac tgtcgaacac gtcgagcatc gacgacgtca
     3541 accgcttcgt gttcggtgcg aagcttcgcg gggcgtacgg cggcgggctg tatgacgaca
     3601 tgatctcgac cgccgaccag taccgggccc tcgcaccatt ccggggctga agttcaaggc
     3661 tcgaggctga gggccaaggc tgaacgcggt tggtttgagc accgctcgcg agcttgcgtg
     3721 tagcgaacgc gtgggtacca tggcgaaagc accgcagatc gccgccagtt ggctccgacc
     3781 tgtgcgccgt tcgaaacccg gcaatcggtt tcctccacga tcacatcgac gaaggaacca
     3841 acatggccgt caccgacgcg aaccgactcg caatgcacaa agacctcacc gccgcactcg
     3901 gcgaagaatc ggcgaacact ctcatggaac acctccccac aaaaggcgct gccgaactcg
     3961 ctaccaaaac cgacctcgac aacctgcgca ccgaactgga cgctcgattc gacaagatcg
     4021 atgcgcgatt cgacaagatc gacgcccggt tcgacaagac cggcaggaat gtctatgcgg
     4081 cccgcctctg ctgcc
//
LOCUS       DAOOMA010000184         4055 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_26667_length_4055_cov_4.136750, whole genome shotgun sequence.
ACCESSION   DAOOMA010000184 DAOOMA010000000
VERSION     DAOOMA010000184.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 4055)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4055)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4055
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26667_length_4055_cov_4.136750"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..726
                     /locus_tag="PLP95_06170"
     CDS             <1..726
                     /locus_tag="PLP95_06170"
                     /inference="COORDINATES: protein motif:HMM:NF024384.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3866 family protein"
                     /protein_id="HPB45422.1"
                     /translation="SELLHRDLPASLGGAVVVATQVHSQVGVVAATIAAIRPGTRVVY
                     VMTDGAALPMALSDLVVSLRDLGAIDATVSAGHAFGSDLEAVTVASALSLAVHVAGAE
                     VVIAGMGPGVVGTGTELGSTAIEAAHIVDTVDALGGRAVMCLRASSGDERSRHQGVSH
                     HTQTALRLARSRPRVAVVEGAGDIDPAHGAAVHVEPPDIEAILDAAGLRITTMGRDVT
                     RDRLFFDAAAAAGALAVRELDGA"
     gene            847..1794
                     /locus_tag="PLP95_06175"
     CDS             847..1794
                     /locus_tag="PLP95_06175"
                     /inference="COORDINATES: protein motif:HMM:NF024676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HPB45423.1"
                     /translation="MAATDSTSSGISKLERLLNLTALLLATRRPLTVEQIGEQIAGYP
                     AEPMSFRRQFERDKDELRQAGVPLELVTLSEWDEREVGYRIPPELYYLPDPELDPDER
                     AALQFAMDAISLRGLEGSQRSDAGLKLELPPSEAMAPMADVSVSAEVGVLREAISSGQ
                     VVRFAYRSAMRTVEPHRLDYQNGRWYLTGRDLDKEAIRSFRVDRFDGSVDPGTPGSFE
                     APTSTRALRLEPWRFGDDPPTAVTLQIDRDHAPVVRDSFGPDAEWTERADGDVEVRCV
                     VSGRRAFRTLVLSMLDHAVIVEPADVRDEFVTYLEGLAR"
     gene            1791..2822
                     /locus_tag="PLP95_06180"
     CDS             1791..2822
                     /locus_tag="PLP95_06180"
                     /inference="COORDINATES: protein motif:HMM:NF024676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HPB45424.1"
                     /translation="MSQRITSADRYRRILAMIPWIVEQGAPSLSDIAERFSITQDQVL
                     ADLDVVLLVGVPPYYPDDYIDVVYESDVVSVRLGDHFRRPLRLTATEALNVLIAGAGL
                     AAAEPENGPLSRAMGKLRVALGVPDGALEVALGPADSEVLATLRSGQQESTSVEIEYY
                     TAGTDTLSQRLIDPHRIYSAEGHWYVAGHCHRAADQRVFRVDRIRSAVASAAHFEVPV
                     HVPEPTVFEADEAKRRLVLDLAADAHWMGTTARAEAVERFDDGRRRVTLAVGGRAWME
                     RVLLEAGPDATIVDSDSDTDTGTAAGAGAGHDAGAGHDVEDRDNWVPATEAIAIRREA
                     ATRILDRYR"
     gene            2908..>4055
                     /locus_tag="PLP95_06185"
     CDS             2908..>4055
                     /locus_tag="PLP95_06185"
                     /inference="COORDINATES: protein motif:HMM:NF021981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S26 family signal peptidase"
                     /protein_id="HPB45425.1"
                     /translation="MPARIQLHDAEYEYVDFGRSLANSLLGRPASAATTTIERPAKQR
                     DTDVKTDRSSPPEPTRPPTPPQRPARPERPARPERPARPERPARPEPAAVSEPVVAAP
                     AVDEPTADDADTRFEIDPTKVAALHLGEPPHHPEPADGDTAPAAGRWSKGIVDAVRFK
                     ISETRSKRDADDVDGPPVPTPGLTFEPVREPTPAPAASGSEDVVPDAANHLDSADVAH
                     TGPDPVDRQDARALSAEAAAEPAEPRLPLAGIALRRERGDEAPFASLQPDVERQIRSR
                     IDAGTAPHPYSELPWSDERDERDVDDDGDIRTLDVSTLPEAVDASPAPATVSTWKQVR
                     DWVLIIGCAIAAALALQTWAVQMFEIPSESMYPTLKVGDRVAVNKAAF"
BASE COUNT          653 a         1419 c         1357 g          626 t
ORIGIN      
        1 tcggagctgt tgcaccggga tctgccggcc tccctgggcg gggcggtcgt cgtggcgacc
       61 caggtccaca gccaggtcgg ggtggtggcg gcgaccatcg ctgcgatacg gcccggaacc
      121 cgcgtcgtct acgtgatgac cgatggcgcc gcgttgccga tggccttgag cgacctcgtc
      181 gtgtcgctgc gcgatctcgg cgcgatcgac gccacggtga gcgccggcca cgcgttcggc
      241 agcgacctcg aagcggtgac ggtggcttcc gctctgagcc ttgcggttca cgtcgccggc
      301 gcggaggtgg tcatcgccgg catgggtccg ggggtggtgg gcacgggcac cgaactcggc
      361 tccaccgcca tcgaggccgc gcacatcgtc gacacggtgg atgccctcgg tggccgagcg
      421 gtcatgtgcc tgcgggcatc gtcgggggac gagcgctcgc ggcatcaggg ggtcagccac
      481 cacacccaga ccgcgctgcg tctggcgcgc agccgtccgc gcgtggcggt cgtcgagggt
      541 gcgggcgaca tcgaccccgc tcatggcgcg gcggtgcacg tcgagccgcc cgacatcgag
      601 gccatcctcg acgcggcagg gttgaggatc accacgatgg gccgcgacgt gacccgcgac
      661 cgtttgttct tcgacgccgc tgcggcggcc ggagcactcg ccgtccgtga actcgatggc
      721 gcctgagggc gacacgtcgg gcgacacgtc ggcagagttt gtgcagaatg cgacaccagg
      781 tgtcgtgttc tgcacaaagt tcgcgcccgg caccgcggtc aagcgtctct aaactcggcc
      841 cccgatatgg cagcgaccga ctccacctcc agcggcatct ccaaactcga gcggctcctc
      901 aacctcacgg cgctgttgtt ggcgactcgg cggccgctca ccgtcgaaca gatcggcgag
      961 cagatcgccg gatatccggc ggagccgatg agctttcgcc gccagttcga gcgcgacaag
     1021 gacgaactac gccaggccgg agtgccgctc gagctggtca cgctgtccga atgggacgaa
     1081 cgcgaggtcg gctaccggat tcctcccgag ctctattacc tgcccgaccc cgaactcgac
     1141 ccggacgagc gcgccgcgtt gcagttcgcc atggacgcga tctcgcttcg cgggcttgag
     1201 ggttcgcagc gttccgacgc cggtttgaaa ctcgagttgc ctccgagcga ggcgatggct
     1261 ccgatggccg acgtgtcggt atcggccgag gtcggggtcc tgcgagaggc gatctcgtcg
     1321 gggcaggtcg tgcggttcgc ctaccgctcg gcgatgcgca ccgtcgaacc ccaccgcctc
     1381 gactaccaga acgggcggtg gtatctcacc ggccgagacc tcgacaagga ggccattcgg
     1441 agctttcggg tggaccgctt cgacggctcg gtcgacccgg gcaccccggg ttcgttcgaa
     1501 gcaccgacgt cgacccgtgc gctgcgcctc gaaccgtggc gattcggcga cgacccaccg
     1561 accgcggtca cgttgcagat cgaccgcgac cacgcccccg tggtgcgcga cagcttcggc
     1621 cccgacgccg agtggaccga gcgcgccgac ggcgacgtcg aggttcgatg cgtggtcagc
     1681 gggcgacgcg cttttcgcac cctggtgctg tcgatgctcg accacgccgt catcgtcgaa
     1741 ccggccgacg tgcgcgacga attcgtgacc tatctcgagg ggttggcccg atgagccagc
     1801 gcatcacttc ggcagaccgc tatcggcgaa tcctggcgat gattccctgg atcgtcgaac
     1861 agggcgcccc atcgctcagc gacatcgccg aacgcttttc gatcactcag gatcaggtcc
     1921 tggccgacct cgacgtggta ctgctggtgg gcgttccgcc gtactacccg gacgactaca
     1981 tcgatgtcgt ctatgagtcc gacgtcgttt cggttcggct cggcgaccat ttccgccggc
     2041 cgctccgatt gacggcgacc gaagcgctca acgtgctgat cgccggagcg ggcctcgcgg
     2101 ccgcggagcc cgaaaatggg ccgctgtctc gcgccatggg aaagctgcgg gtggccctcg
     2161 gcgtgcccga cggcgccctc gaggttgcgc tgggacctgc cgattcggag gtgttggcga
     2221 cgttgcggag cggtcaacag gaatcgacca gcgtggagat cgagtactac accgccggta
     2281 ccgacacgtt gtcacaacgc ctgatcgacc cccaccggat ctactccgca gaggggcact
     2341 ggtatgtggc cgggcattgt caccgggccg cagaccaacg cgtgtttcgc gtcgatcgga
     2401 tccgcagcgc cgtcgcgtcc gcggcccact tcgaggttcc ggtccacgtc cctgaaccca
     2461 cggtgttcga ggccgacgaa gcgaagcggc gcctggtgtt ggatctcgct gcggatgcgc
     2521 actggatggg aacgaccgcc cgagccgagg ccgtcgaacg attcgacgac ggacgccgac
     2581 gcgtcacctt ggcggtcggt ggacgggcct ggatggagcg ggtactgctc gaggccggtc
     2641 cggacgccac gatcgtggat tccgactccg acaccgacac cggcaccgcc gctggtgcgg
     2701 gcgccggcca tgatgcgggc gccggccatg atgtcgagga ccgggacaac tgggtgccgg
     2761 caaccgaggc gatcgccatc cgacgcgaag cggccacgag aattctcgat cgctaccgtt
     2821 agcccggcgc cgaggagtcg gtcctcgcgg tgaaatggtc caaacggcgc ggccgcgttt
     2881 gtaaagaccg ccgatagcct gatcatcgtg ccggcccgca tccaacttca tgatgccgaa
     2941 tacgagtacg tcgacttcgg tcgctcgctc gccaactcgc tgctcggacg accagcatcc
     3001 gcagcgacga cgacgatcga acgcccggcg aaacagcgcg acaccgacgt caagaccgac
     3061 cgatcctctc cgccggagcc gactcggccg ccgacaccac ctcagcggcc agctcgaccg
     3121 gagcggcctg ctcgaccgga gcggcctgct cgaccggagc ggcccgctcg accggagccg
     3181 gcggccgttt ccgaacctgt cgtcgccgcg cccgcggtcg atgaaccaac cgctgacgat
     3241 gccgacaccc gtttcgagat cgaccccacg aaggtggcgg cactccacct cggcgagccc
     3301 ccgcaccatc cagaaccagc cgacggcgac accgcaccgg cggctggccg ttggagcaag
     3361 ggaatcgtcg atgccgtgcg cttcaagatc tccgaaaccc ggtccaagcg cgacgccgac
     3421 gacgtcgacg gacctccggt gccaaccccg gggctcacgt tcgaacccgt gcgtgaaccg
     3481 acgcccgcac cggccgcgtc cggctccgaa gatgtcgtgc cggatgccgc caaccacctc
     3541 gattccgccg acgtcgcaca caccggcccc gatccggtcg atcgccagga cgcccgtgcg
     3601 ctcagcgccg aggccgccgc tgagcccgcg gagccgcgtt tgccgctcgc tggaatcgcg
     3661 ctgcgacgcg aacgcggtga cgaggccccg ttcgcctcgt tgcagcccga cgtggaacga
     3721 cagattcgca gcagaatcga cgccggcacc gcgccccacc cgtacagcga actgccgtgg
     3781 agcgacgagc gcgacgagcg cgacgtcgac gacgatggcg atattcgcac cttggacgtt
     3841 tccaccctgc cggaagcggt cgacgcctcg ccggcaccgg caaccgtctc gacgtggaag
     3901 caggtccgcg actgggtgct catcatcggt tgcgccatcg ctgccgcgct cgcgctgcag
     3961 acctgggcgg ttcagatgtt cgaaatcccg tcggagtcga tgtatccgac gctcaaggtt
     4021 ggcgatcgcg tcgcggtgaa caaggccgct ttcaa
//
LOCUS       DAOOMA010000185         4033 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_26880_length_4033_cov_4.432127, whole genome shotgun sequence.
ACCESSION   DAOOMA010000185 DAOOMA010000000
VERSION     DAOOMA010000185.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 4033)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4033)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4033
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26880_length_4033_cov_4.432127"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..555)
                     /locus_tag="PLP95_06190"
     CDS             complement(<1..555)
                     /locus_tag="PLP95_06190"
                     /inference="COORDINATES: protein motif:HMM:NF037125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB45426.1"
                     /translation="MALHTDIPTRDEIERLIGSTAEPAVSIYLPTHRVTQETAKDRLR
                     LRDLTGQAVEQLHAAGASGDDIADIEEHLLHLVDDDDDFWLEQADSLAIFAAPGRFET
                     HRLPNHLSEALEVSDRFYVTPLMRAVTFPHAAWVLAISQGDVRLLELASSGPVREVRV
                     DGMPRDAWESDGNKIHKAREAAYAR"
     gene            838..1542
                     /locus_tag="PLP95_06195"
     CDS             838..1542
                     /locus_tag="PLP95_06195"
                     /inference="COORDINATES: protein motif:HMM:NF014090.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005384 - manganese ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030026 - cellular manganese ion
                     homeostasis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VIT1/CCC1 transporter family protein"
                     /protein_id="HPB45427.1"
                     /translation="MANDLDKLADVWAWVRDPGTVRGWIIDANDGIIATAGILQGFAG
                     AGAGDRLLIFTATAATIAGGISAGGAKWAEDASERESQRRLARLELADIEQNPEGEIA
                     ELTAHWCARGLEPELAHVVAERLTAHDALSAQLESEYGFDKPMPALIPVLSGLGTWLA
                     FVGGAMIPLLITYFAPLNIETWAILGAVIVALTLTAVASSRAAHLSAASTLGRSLIVG
                     ALTLSISYVAAELLLN"
     gene            1694..2467
                     /locus_tag="PLP95_06200"
     CDS             1694..2467
                     /locus_tag="PLP95_06200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPB45428.1"
                     /translation="MSLLDVTDIGVQFGGVTALAGLSFTIDEGQICALIGPNGAGKTT
                     LFNVVSRLYQPSEGTVTFDGTDLLALPPHRIARLGIARTFQNLALVPGLSVLDNVIIG
                     AHPRTTGGFWAAALRFPVAAQERRTREAAMSLLADLDLARFADRPCEGLPFGTLKRIE
                     IARSLMSQPRILLMDEPASGLTHGEVDELGGLVRMIRDRHNLTVLLVEHHMGMVMSVS
                     DKVVVMELGRKIAEGDPAAVQNDPRVIAAYLGEPVAGGE"
     gene            2464..3174
                     /locus_tag="PLP95_06205"
     CDS             2464..3174
                     /locus_tag="PLP95_06205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPB45429.1"
                     /translation="MMSLFQVTNLEAGYGIVDVLFGLDFTVEAGEAVVILGANGSGKT
                     TTLRAVSGMLAAKGSIHFAGSEVLGKRPDQLLSLGIAHVPQGRGTIVDLTVEENLRVG
                     AYTRTDGNVEAEIARWYDVFPRLAERRTQLAGSMSGGEQQMLAIARAFVSNPKLVLLD
                     EPSLGLAPLVTREVFERVGDLVRETGTAVLLVEQNANLALDFASRGYVLEAGRIVSAG
                     SADELRADDSIRKAYLGG"
     gene            3176..>4033
                     /locus_tag="PLP95_06210"
     CDS             3176..>4033
                     /locus_tag="PLP95_06210"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HPB45430.1"
                     /translation="MGQFMNYLYIGLGNGAIYATLAVGLVVIYRASGLMNFAQGEMAM
                     FTTYFVWMFSDLGLPLWLAVLAGMAAAFLLGVVTFQLLVRPIGDPAQKPLAVVIVTIG
                     MFLAFSAAALLIWGTDDRRIPTFWSGSFGLLGGTMQWQKLLALLVLAAESLVLFILFK
                     RTKIGLAMRAVASNPESAGLCGIPVSRILMIGWGIAAALGAVAGSLWASNSLAVNVNL
                     MPIVLIYAFAAIIIGGFDSIAGAVVGGLAIGIVTDVLPKYLSVFEKMPLAPALIVMLL
                     VLLVRPQGLF"
BASE COUNT          662 a         1385 c         1266 g          720 t
ORIGIN      
        1 gcgggcgtaa gccgcttccc tcgccttgtg gatcttgttg ccgtccgact cccacgcatc
       61 acgaggcatc ccgtcgacgc gaacctcgcg gaccggcccc gacgaggcga gttcgaggag
      121 ccgcacgtct ccctgtgaga tggcgagcac ccacgcggcg tgcggaaagg tgaccgcacg
      181 catgagcggc gtgacataga agcggtccga aacctcaagg gcctcgctca agtggttcgg
      241 caagcggtga gtctcgaaac gcccgggtgc cgcgaagatc gccaagctgt ccgcctgctc
      301 caaccagaag tcgtcatcgt cgtcgacgag gtgcaggagg tgctcctcga tgtccgcgat
      361 gtcgtcgccc gacgctccgg ccgcatggag ctgctcaacg gcttgccccg tgagatcgcg
      421 aaggcgaagc cggtccttgg ctgtctcctg ggtgacgcgg tgggtcggga ggtagatcga
      481 caccgcaggt tcagcggtac tgccgatcag acgctcgatc tcgtcgcggg tggggatgtc
      541 ggtgtgaagc gccatgtggt ggccttcctt tcaaagtgga gagagggaac cttcgcgggc
      601 tgtcggggaa gcggttgcgc atcgcgctgc gtgcgcgctc cctcgctgag aactccaccg
      661 acaaacaagc cgttacggct gtcgccccgc accagcagtc ttacccaacg accgctcttt
      721 cgcacgccgg gggtgcggca gtacgttgcc gctcagccct caccattcag ccctcacctc
      781 ggtcgctgcg aactcgtgac ggcgctgcga gaaacccgga acataaggag tactcggttg
      841 gcaaacgacc tggacaaatt ggccgacgtc tgggcatggg tccgcgaccc tggaaccgtg
      901 cggggctgga tcatcgacgc aaacgacggg atcatcgcca ccgccggaat actccagggc
      961 ttcgccggcg cgggggccgg agaccgcctg ttgatcttca ccgccaccgc agcgaccatc
     1021 gccggcggga tcagcgcggg cggcgcgaaa tgggccgagg acgcatccga acgcgaatcc
     1081 cagcgacggc tcgcccggct ggaactcgcc gacatcgaac agaaccccga gggcgagatc
     1141 gcggaactca ccgcccactg gtgtgcccgc ggcctcgaac ccgaactcgc ccacgtcgtc
     1201 gccgaacggc tgaccgcaca cgacgcgctg agcgcccaac tcgagtcgga gtacgggttc
     1261 gacaagccga tgccagccct catcccggtg ctctcaggcc tgggcacctg gctggccttc
     1321 gtcggcggtg cgatgatccc gttgctcatc acctacttcg ctccgttgaa catcgaaacc
     1381 tgggcgatcc tcggggccgt catcgtcgcc ttgaccctga ccgcggtcgc ttcgtcccgg
     1441 gcggcccacc tctctgccgc cagcacgctc ggccgaagcc tgatcgtggg cgcgctcacg
     1501 ctctccatca gctacgtggc cgccgaactt ctcttgaact gacctcgaac gcgtcgatga
     1561 gtcgagtcgg tcgccgttcg tcgatggcgt tgcggtcacc gccgacattt ccaaagattc
     1621 ccgccgcgtt cccgctacgc tgtgccaacc acggagccgg accgggttcg aggatcgagg
     1681 agagggggac agcttgtcgt tactcgacgt caccgacatc ggagtccaat tcggcggggt
     1741 gacggccctc gccgggctct cgttcaccat cgacgaaggt cagatctgcg cgctcatcgg
     1801 acccaacggt gccggaaaga ccacgctgtt caacgtcgtg tcgcgcctct atcaaccgtc
     1861 ggagggaacg gtgaccttcg acggcaccga cctgttggct ctgccgccgc atcgcatcgc
     1921 ccggctcgga atcgcccgca ccttccagaa cctggcgctc gtgcccgggc tttcggtgct
     1981 cgacaacgtg atcatcggag cgcacccccg cacgaccggc gggttctggg cagcagcact
     2041 gcggttcccg gtggcggcac aggaacgcag aacccgcgag gccgccatgt cgctgctcgc
     2101 cgacctggat ctcgccagat tcgccgaccg cccctgcgaa ggcctcccat tcggcacact
     2161 caaacgcatc gagatcgcca gatccctcat gtcgcaaccg cggatcctgc tgatggacga
     2221 gccggccagt ggcctcaccc acggcgaggt cgacgagctc ggagggctcg tacggatgat
     2281 ccgcgaccgc cacaacctca cggtgctgct cgtcgaacac cacatgggga tggtgatgag
     2341 cgtctcggac aaggtcgtcg tgatggaact cggacgaaag atcgccgaag gcgacccggc
     2401 cgcggtccaa aacgatcctc gcgtcatcgc tgcctacctg ggcgaacccg tcgcgggagg
     2461 cgaatgatgt cgctgtttca ggtcaccaac ctcgaagccg gctacggcat cgtcgacgtg
     2521 ctcttcggcc tcgatttcac cgtcgaagcc ggcgaggcgg tcgtcatcct cggtgcgaac
     2581 ggctcgggaa agaccaccac gcttcgggcg gtctcgggca tgttggcggc caagggttcc
     2641 atccacttcg ccggctccga ggtgctggga aagcgccccg atcaactgct gtcgctcggc
     2701 atcgcccacg tgccccaagg ccgtggaacg atcgtcgacc tcaccgtcga ggagaacctt
     2761 cgcgtcggcg cctacacccg caccgacggc aacgtcgagg ccgagatcgc ccgctggtac
     2821 gacgtctttc cccggctcgc ggaacgccgg acccaactcg ccggctcgat gtcgggtggc
     2881 gaacagcaga tgctcgccat cgcccgcgcg ttcgtgagca acccaaagct ggtgttgctc
     2941 gacgagccgt ccctcggcct cgccccactc gtcacccgag aggtgttcga acgggtgggc
     3001 gaccttgttc gcgagaccgg caccgccgtc ctactcgtcg aacagaacgc caacctggcg
     3061 ttggacttcg ccagccgcgg ctacgtgctc gaagccgggc ggatcgtctc ggccggctcc
     3121 gccgacgagc ttcgagccga cgactcgatt cgcaaggcgt acctgggagg ctgagatggg
     3181 ccagttcatg aactacctct acatcggcct cggcaacggc gcgatctacg ccaccctcgc
     3241 cgtcgggctc gtcgtcatct accgggcgtc ggggttgatg aacttcgcgc agggcgaaat
     3301 ggcgatgttc accacctatt tcgtctggat gttttccgat ctcgggctgc ccctgtggct
     3361 ggcggtgttg gcaggcatgg cggccgcgtt cctcctcggt gtcgtcacgt tccaactcct
     3421 cgtgcgaccg atcggagacc cggcacaaaa gccgctcgcc gtggtgatcg tgaccatcgg
     3481 catgtttctg gcgttcagcg cggccgccct gctcatctgg ggtaccgacg atcgtcgcat
     3541 tccgaccttc tggtccggga gcttcgggct gctcggcggc accatgcagt ggcagaaact
     3601 cctcgcgctg ctggtgctcg ccgccgagtc gctggtgctg ttcatcttgt tcaaacgcac
     3661 caagatcggg cttgcgatgc gagccgtcgc ttccaacccc gaatcggccg ggctctgcgg
     3721 gatcccggtt tctcgcatcc tcatgatcgg ttggggaatc gccgcggccc tcggcgccgt
     3781 cgccgggtcg ctgtgggcgt ccaattcgct ggccgtcaac gtcaacctga tgccgatcgt
     3841 gctcatctac gcgttcgccg cgatcatcat cggcggtttc gactcgatcg ccggagcggt
     3901 cgtcgggggc ttggccatcg gcatcgtcac cgatgtcctc cccaagtacc tgtcggtctt
     3961 cgaaaagatg ccgctcgcac ccgcgttgat cgtgatgttg ctggtcctgc tcgtgcgtcc
     4021 acaagggttg ttc
//
LOCUS       DAOOMA010000186         4032 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_26885_length_4032_cov_4.374151, whole genome shotgun sequence.
ACCESSION   DAOOMA010000186 DAOOMA010000000
VERSION     DAOOMA010000186.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 4032)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4032)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4032
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26885_length_4032_cov_4.374151"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..483)
                     /locus_tag="PLP95_06215"
     CDS             complement(<1..483)
                     /locus_tag="PLP95_06215"
                     /inference="COORDINATES: protein motif:HMM:NF016330.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SWIM zinc finger family protein"
                     /protein_id="HPB45431.1"
                     /translation="MNPDLPDDLRRAVDAVTDEVLHRTYSDATIQRGVRYARQDRAMV
                     DMVEDDFGAYLGRVQGSDREPYNTWFSVDDVRGSVRVSDDCDCPLGGRCKHVVALILS
                     ARGYGYGSRPSDTAAGAAQLALSDDTTLISGSGLAVPAPSVSAAFRRPTWRGALQDFA
                     R"
     gene            complement(554..1189)
                     /locus_tag="PLP95_06220"
     CDS             complement(554..1189)
                     /locus_tag="PLP95_06220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB45432.1"
                     /translation="MLTAGDRRSPEDRPVAIRVFVDAPYADAKSLLLQMIARQSRCRT
                     VFLTGLDMSTVVGFPADVAAVHVLFTSLLVQAQAALNNAARTAAPGTRTRSAGFRAAF
                     YQAFAQRIDERLSEASKHASDAAAAEYGDAFLPVLAGRQQEVDDLFNDRFTNLTSGSM
                     RRSYDRAGWASGQLAADTAKLHRGDLTDEATDDPTPPEPAALRPFSDNQWS"
     gene            complement(1355..2149)
                     /locus_tag="PLP95_06225"
     CDS             complement(1355..2149)
                     /locus_tag="PLP95_06225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB45433.1"
                     /translation="MTRPYPQRDVAAGMNTACAGASLSGVRSSIATMGANNRARRAAK
                     SKARQQARREHAQRDHGQRDRSGRVSGGATGGTGGNSYGGRQHDHDTYDFNDPFARER
                     ILTEQVDALWHEVYGAVSRDRSSEPARRRLAQLDASAVNRGAERLLLLQIDQVWAHGW
                     QPAELVRHVTRVTKNRTTRDIVSALVAADYLRRPPTSIDARWRAQIESLDIVQGATTR
                     DWITTALRGAATRAEALLSISETFATLRLGRLDVLIPPPGADSSTT"
     gene            2189..2953
                     /locus_tag="PLP95_06230"
     CDS             2189..2953
                     /locus_tag="PLP95_06230"
                     /inference="COORDINATES: protein motif:HMM:NF025091.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase/phosphoenolpyruvate mutase
                     family protein"
                     /protein_id="HPB45434.1"
                     /translation="MTDLASRAQALLDMHHSPATLVLPTVWDVWSARTVVDAGFGALT
                     IGSHPVADSRGQRDNEGMTIDDALDSVRRITAAVDVPVSADMESGYALAPAELVDRVL
                     EAGVAGINVEDTVHSEGRVRSPAEHAEYIGAIRAAADSAGVALVINARTDAFIGAVES
                     YADPLAEAIARLKLCEEAGARCVYPVKAPSREAIESLLSELSGPVNVLANPVDGSAAG
                     SLADLQALGVHRVSMGPLLQKSLAPRLADLVGPWLG"
     gene            3015..>4032
                     /locus_tag="PLP95_06235"
     CDS             3015..>4032
                     /locus_tag="PLP95_06235"
                     /inference="COORDINATES: protein motif:HMM:NF012363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase fold domain-containing
                     protein"
                     /protein_id="HPB45435.1"
                     /translation="MKHSHRLVTLAVVSVLVLSGCKSDPGGTVDSAGASADEPTTTTT
                     TLLQGPPVAPDALDISFTEAPFAYDGDVSFVSDLRCGPAERNVVDVAVPRSVDEIEAA
                     RSEGSADLPLVVHIHGGGFIGGDKAEVWTEDAEEITTYLANGVAVASLNYTLLASAAD
                     GGVKTSMGDAAACLQFLRYNGSATFGIDPDLVVLRGGSAGAGTSLWLATHDDLADPTS
                     SDPIEQQSTKPIAVAVVETQATYDLDRWGTDVFGDYTELFANQTLVQLAEAFGLADRL
                     LSFYGISDSSQLATPEIEAYRADVDILALMDPNDPPAWVRNTREEEVLPVSVGLLFHH
                     GYHAR"
BASE COUNT          638 a         1284 c         1414 g          696 t
ORIGIN      
        1 cctggcgaag tcctggagcg ctcctcgcca cgtgggccgc ctgaatgccg cggacacgga
       61 gggggcgggg acggcgaggc cggaaccgga gatgagggtg gtgtcgtcag agagcgcgag
      121 ttgggcggcc cccgctgcgg tatcggaggg tcgggatccg tatccgtaac cgcgggccga
      181 caagatgagc gctaccacgt gtttgcaacg gccaccgagc ggacagtcgc aatcatcgct
      241 gacacgcacg gagccgcgga cgtcgtccac cgagaaccag gtgttgtacg gctcgcgatc
      301 cgagccctgg acccggccca aatacgcccc gaaatcgtct tcgaccatgt cgaccatggc
      361 ccggtcctgg cgtgcatagc gcacaccccg ctggatcgtc gcgtcggagt aggtgcgatg
      421 cagcacctcg tcggtcacgg cgtcgacagc cctgcggagg tcgtctggaa ggtcgggatt
      481 cacgggccgc gagcctacgc gtcgatccaa gcgttctccg cccgactcgt tctccgataa
      541 ccagtggtcg tgatcacgac cactggttat cggagaacgg gcggagtgcg gccggctcgg
      601 gtggggttgg atcgtcggtg gcctcgtcgg tcaggtcgcc gcggtgcaac ttggcggtgt
      661 cagcggcgag ttgcccactc gcccaccccg cccgatcgta ggagcggcgc atcgaaccgg
      721 aagtgaggtt cgtgaatcga tcgttgaaca ggtcgtcgac ctcttgttgg cgaccggcga
      781 gcaccggaag gaacgcatcg ccgtactcgg cggccgccgc gtcggaagcg tgcttcgatg
      841 cctcgctgag gcgctcgtcg atgcgctgag cgaaagcctg gtagaaggct gctcgaaatc
      901 cggccgaacg cgttcgggtg ccgggcgcag cggtgcgcgc cgcgttgttg agcgccgcct
      961 gagcctgcac gagtagcgag gtgaacaaca cgtgcaccgc agcgacgtcc gcagggaacc
     1021 cgacgacggt cgacatgtcg aggccggtga gaaacaccgt tcgacagcgc gactgtcggg
     1081 cgatcatctg caggagcagc gacttggcat cggcataggg cgcatcgaca aagacccgga
     1141 tggcgaccgg acggtcctcg ggcgaacggc ggtcgccggc cgtgagcatt gcctgatcga
     1201 tggcatggcg gacgatgagt tcctgggctt tggcggtgaa cgctgcggcc tcggcctcga
     1261 actcggtcga ctctgccttc gccagcagat tgcggactcg ttcgatgacc gggtcggcgg
     1321 cggtgtcggg tgcgacggcg ccaccgcccg gcggtcaggt cgtcgagctg tcggcgccag
     1381 gaggggggat caggacatcg aggcggccga gcctcagtgt tgcgaaggtc tcactgatcg
     1441 acagcagcgc ctcggcgcga gtggctgcgc cgcgtagcgc ggtggtgatc cagtcgcgtg
     1501 tcgtcgcccc ttggacgatg tcgagggact cgatctgggc gcgccaccga gcatcgatgc
     1561 tggttggcgg gcgcctcagg tagtcggcgg cgaccagggc gctgacgatg tcgcgggtcg
     1621 tgcgattttt ggtcacgcgt gtgacgtggc ggacaagttc tgcgggttgc catccgtggg
     1681 cccacacctg gtcgatttgg aggaggagca gtcgctctgc gccccgattg acggcggagg
     1741 catcaagctg agcgagccgt cgacgtgccg gttcgctgct ccgatcccgc gacacagcgc
     1801 cgtagacctc gtgccataac gcgtcgacct gctcggtcag gatccgctcg cgcgcgaacg
     1861 gatcgttgaa gtcgtaggtg tcgtggtcgt gctgtcgccc gccgtacgag ttgccgcccg
     1921 tgccgcccgt tgccccgccc gagacgcggc ccgaccggtc gcgttgtcca tggtcgcgtt
     1981 gcgcatgctc gcggcgggcc tgttggcgtg ccttgctctt cgcggcgcgg cgcgcccggt
     2041 tgttcgctcc catggtggcg atgctagagc ggacccccga cagcgaggcg ccggcgcaag
     2101 ctgtgttcat gcccgcggcg acgtcgcgtt gagggtacgg tcgcgtcacc ggagtaattc
     2161 cgtcgttgtc tagaacaaag gagtcaccat gaccgatctc gcgagtcgcg cacaggccct
     2221 gctcgacatg catcattcgc cagccacgct ggtgcttcca accgtgtggg acgtgtggtc
     2281 ggcccgcacg gtcgtcgacg ccggattcgg tgcgctcacc atcgggagtc accccgtcgc
     2341 cgattctcga gggcagcgcg acaacgaagg gatgacgatc gatgacgcac tcgacagcgt
     2401 tcgtcgtatc accgccgcag tcgacgtgcc ggtgagtgcc gatatggaat cgggttatgc
     2461 gctggccccg gccgaactcg tcgaccgggt tctcgaggcc ggtgtggcag gcatcaacgt
     2521 cgaagacacc gtgcacagcg agggccgggt acggtcgccc gccgagcacg cggagtacat
     2581 cggagcgatt cgcgccgcgg ccgattccgc cggggtcgcg ctggtcatca acgctcgcac
     2641 cgatgcgttc atcggggccg tcgagagcta cgccgacccc cttgcggagg ccatcgcccg
     2701 cctgaagctg tgcgaggagg cgggagcgcg gtgcgtctat ccggtgaagg ccccgagccg
     2761 cgaggccatc gagtcgttac tttcggagtt gtcgggaccg gtcaacgtgt tggcgaaccc
     2821 ggtcgacgga tcggccgcgg gatcgttggc cgacctgcag gccctcgggg tgcatcgcgt
     2881 gtcgatgggg ccgctgttgc agaaatcgct ggcgccgcgc ctggccgacc tcgtcggacc
     2941 gtggctgggg tgacccgccc atcggcagca acgtcggcaa tgtcggtgag ctcgatctgc
     3001 cagtatgcgg acatatgaag cattcacatc gtctggtgac gctcgccgtg gtttccgttc
     3061 tggtgctgtc cggttgcaag tcggaccccg gcgggacggt cgactcggcc ggggccagcg
     3121 ccgacgagcc cacgaccacg accacgacgc tcctgcaggg tccgcctgtg gcgcccgacg
     3181 ccctcgacat cagcttcacc gaggcgccgt tcgcctacga cggagatgtg tcgttcgtgt
     3241 cggatctgcg ttgtggtcct gccgagcgca acgtcgtcga cgtggccgtt ccccgaagtg
     3301 tcgacgagat cgaggcggcg cgctctgagg gatcggccga tctcccgctc gtggtccaca
     3361 tccatggggg cggcttcatc ggcggtgaca aggcggaggt gtggaccgag gatgccgagg
     3421 aaatcacgac ctacctggcc aacggtgtcg ccgtcgcgtc gctcaattac acgctgttgg
     3481 cctcagctgc tgacggtggc gtgaagacct cgatgggcga cgccgcggca tgtctgcagt
     3541 tcctgcgata caacggctcg gccacgttcg ggatcgaccc ggatctggtg gtgttgcgcg
     3601 gcggaagtgc tggcgctgga acatcgctgt ggctggcgac ccatgacgat ctcgcggatc
     3661 cgacgagttc ggatccgatc gaacaacagt cgaccaaacc catcgctgtc gcggtggtcg
     3721 aaacccaggc cacctacgac ctcgaccggt ggggtaccga tgtcttcggc gactacaccg
     3781 aactgttcgc caaccagacg ctcgtgcaac tcgccgaggc gttcggcctc gcagaccgac
     3841 tcctgtcgtt ctacggcatc agcgactcca gccaactcgc cacacccgag atcgaggcat
     3901 accgcgcaga cgtcgacatc ttggccctca tggacccgaa cgacccgccg gcatgggtgc
     3961 gcaatacccg tgaggaggag gtgttgcccg tatcggtcgg cctgttgttt caccacgggt
     4021 accacgccag gg
//
LOCUS       DAOOMA010000187         4029 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_26916_length_4029_cov_4.395068, whole genome shotgun sequence.
ACCESSION   DAOOMA010000187 DAOOMA010000000
VERSION     DAOOMA010000187.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 4029)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4029)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4029
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26916_length_4029_cov_4.395068"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..781)
                     /locus_tag="PLP95_06240"
     CDS             complement(<1..781)
                     /locus_tag="PLP95_06240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB45436.1"
                     /translation="MTTWERNVTEVVWESQTTPGVTWLTVVGTSESTPAVRLLPTPLG
                     NPGQVAAVNDDGTGTEWVDQAGGGGGLSDDQPLPLGAASPGVSDEASRSDHRHPMPSA
                     SDVGAATPASVTSAIADAKTELRGTAPANGDTLGELHDRIAVVEALGSLATDAELIAA
                     VTALIGSADPSGDTLGELQALIGARLAKAANLADLSNPATARTNLGLGNVDDTSDADK
                     PISTATQAALDLKAPLASPALTGTPTAPTAPPGTDSTQIATT"
     gene            complement(778..1152)
                     /locus_tag="PLP95_06245"
     CDS             complement(778..1152)
                     /locus_tag="PLP95_06245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB45437.1"
                     /translation="MSCCDATATDTVMTHRDIWFQIWVTSTDVSDHTEPIITITDSSG
                     TVLVTSEDEGVHLDGSEEYEIPPTDFDQGRWSWWVDSSERESWPPMIDVWGKAQVAGA
                     DQTWKIGTYRVRSERAPREAGS"
     gene            complement(1164..2030)
                     /locus_tag="PLP95_06250"
     CDS             complement(1164..2030)
                     /locus_tag="PLP95_06250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB45438.1"
                     /translation="MRALIRDSTGYRYRGAVGDDVVFTSKTDYADPGSVDTVVDAELA
                     EATDAENTVAIGMAIAPRNDDDSPFDDFDLGDAVSAANIDGTHEQHRTVAIPVTESPL
                     GVVRFGLELNARVENEAARKQRWLEQITPGALSGRADSVSALDLGAGVPFGPLRTDGW
                     VFNQNGPVVAELDYSDPEDEGHSDDWPVDEPILMYRVRWALTQAGDTATVVLLRINGG
                     GPFNTGAPDYVPFFHAITIPAGATAPDDDSNDGQYTNQIAGKGDTIRAAVYQAGDYAR
                     GLVVRVKHTTQT"
     gene            complement(2027..3352)
                     /locus_tag="PLP95_06255"
     CDS             complement(2027..3352)
                     /locus_tag="PLP95_06255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB45439.1"
                     /translation="MTPGPEAVEPPQRRIQLKLYEADGTFVRDLTTDLTGTRAFTPEL
                     PGIGAFGTAAFSIPLFVTSEALSEMVPNVEATDLTRGRIVRYYLDGELRFAAVIRPRR
                     QTSASSKGHSALVRSLRMEGVLSEWDRAVLPPHPGAVYFLGGEIRNFGWMSPEADIDD
                     LDDAVFLRPVFADGYSHPDPWVDGFGYVFDASTHRYFVYDQEVDEADAPLAVSVHQAF
                     ADQGTGWVNGVSMGQTDGPPSSAWGQTHHGGARLPEGVTRWAFEVQGLPGSPDPRWAA
                     TAFAINDATTGSMNGDTILWRTGYITGVTPYPWKASATPQGPTAKRIIRSALTQVQEE
                     QEKLLGWSIEGDDDSLDANGNELDRIAHVPFRVGCKLGSEFLLGMAKSWCDLDVDYAT
                     KTLRVYRWRERGNFHTSPASAPVFSDAAFAPVDGRLPNVLDMVHEERIR"
     gene            complement(3349..3825)
                     /locus_tag="PLP95_06260"
     CDS             complement(3349..3825)
                     /locus_tag="PLP95_06260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB45440.1"
                     /translation="MADTSGGQLTFDGTSGPFHIVTDPWWPEDDALTPLWFGLDLEGD
                     DTQVGLGDYILNPVGWAPTIVQIPMWFAGHIDHEGVPHSNVIEGFESNLNYWRTELLD
                     PTVVARTGTLTMPSGESRTATASRFRLSGRGRKRPAGWPLMFEFRLTAPFAPEAGS"
     gene            complement(3818..>4029)
                     /locus_tag="PLP95_06265"
     CDS             complement(3818..>4029)
                     /locus_tag="PLP95_06265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB45441.1"
                     /translation="RRQPRSDSPTRPRSSPNSALRQDVANLERVVYAPTIHARPDQVA
                     PTMNEALRVARADSWVTTGQDISRG"
BASE COUNT          624 a         1331 c         1380 g          694 t
ORIGIN      
        1 cggtggtcgc gatctgtgtc gagtctgtgc caggcggcgc ggtcggtgcg gtcggggtgc
       61 cagtcaaggc gggggacgcc agcggggctt tgaggtcgag cgccgcctgt gttgccgtgc
      121 tgatcggctt gtcagcatcc gacgtgtcat cgacgttccc gaggcccagg ttggttcggg
      181 cggtcgccgg gttcgacagg tctgcgaggt tcgctgcttt tgcgagccgt gcgccgatca
      241 gcgcctgtag ttcgccgagc gtgtcccctg acgggtccgc cgacccgatc aacgctgtga
      301 cagcagcgat cagttcagcg tcggttgcga gagaccccaa cgcctccacg acagcgatcc
      361 gatcgtgcaa ttcgccgagc gtgtcaccgt tcgccggtgc ggtacctcgc agttcggttt
      421 tcgcgtccgc gatagcggag gttaccgacg ccggggtcgc agcgcctaca tcggacgccg
      481 acggcatcgg gtgacgatga tccgaccgtg acgcctcatc ggaaaccccc ggcgaagcag
      541 cacctagagg cagcggctgg tcatcggaca ggccaccgcc gccacccgcc tggtcgaccc
      601 actccgtccc ggtgccgtcg tcgttgaccg ccgccacctg acccgggtta ccgagtggag
      661 tcggcaacag tcgcaccgcg ggcgttgatt cggacgtgcc gacgaccgtc agccatgtga
      721 cgccaggcgt ggtctgggat tcccacacga cctcggtgac gttgcgttcc caggtcgtca
      781 tgagcccgcc tctcgcggtg cacgctccga acgcacccgg taggtaccga tcttccacgt
      841 ctgatcggca cccgccacct gggccttgcc ccacacgtcg atcatcgggg gccacgactc
      901 gcgttcggac gagtcgaccc accacgacca gcggccctgg tcgaaatcgg tcggcgggat
      961 ctcgtactcc tcggacccgt cgaggtgcac cccctcgtcc tcggaggtga cgagcacagt
     1021 cccggacgag tcggtgatcg tgatgatcgg ttcggtgtga tccgacacgt ccgtcgacgt
     1081 cacccagatc tggaaccaga tgtcgcggtg ggtcatcacc gtgtcggtcg cggtcgcgtc
     1141 acaacaggac atgcggcctc ccttcaggtc tgcgtcgtgt gcttcacgcg gaccacaagg
     1201 ccccgcgcgt agtcacccgc ctggtacacg gccgcccgga tcgtgtcgcc cttcccggcg
     1261 atctgattcg tgtactggcc gtcgttcgag tcgtcatccg gcgcagtcgc cccggccggg
     1321 atcgtgatgg cgtggaagaa cgggacgtag tccggcgccc ccgtgttgaa cggcccgccg
     1381 ccgttgatgc gcaggaggac caccgtcgcc gtgtcgccgg cctgcgtgag tgcccagcgg
     1441 acccggtaca tgaggatcgg ttcgtccacc ggccagtcgt cggagtgccc ttcgtcctcg
     1501 gggtcggagt agtcgagctc ggccaccacc ggcccgtttt gattgaacac ccacccgtca
     1561 gtccgaagcg gaccgaacgg gacgcccgct ccaaggtcca gggccgacac ggagtcggca
     1621 cgaccagaga gggcgcctgg ggtgatctgc tcgagccacc gctgctttcg tgcggcttcg
     1681 ttctcaactc tggcgttgag ctcgaggccg aatcggacga ctccgagcgg agactcggtg
     1741 acggggatcg cgacggtgcg atgctgttcg tgggtgccgt cgatgttcgc ggcgctgacg
     1801 gcatcgccga gatcgaagtc gtcgaacggt gagtcgtcgt cgttgcgggg ggcgatcgcc
     1861 ataccgatgg cgacggtgtt ctcggcgtcg gttgcctctg cgagctcggc atcgacgacc
     1921 gtgtcgacgg agccgggatc cgcatagtcg gtcttggacg tgaacacgac gtcgtcaccg
     1981 acggctcccc gatagcggta gccggtggag tcgcggatga gcgccctcac cggatgcgct
     2041 cctcgtgcac catgtcgagc acgttcggca gacgcccgtc gacaggggcg aacgccgcgt
     2101 cgctgaacac gggggcggag gctggtgagg tgtggaagtt gcctcgctcg cgccaccggt
     2161 acacccgaag cgtcttcgtg gcgtagtcga cgtcgaggtc acaccacgac ttggccatgc
     2221 cgagcaggaa ctcggagccc agcttgcatc cgaccctgaa cgggacgtgt gcgatgcggt
     2281 cgagttcgtt gccgttcgcg tccaacgagt cgtcgtcgcc ctcgatcgac cagccgagca
     2341 gcttctcctg ttcctcctgg acctgcgtga gtgcggatcg aatgatgcgc ttcgccgtcg
     2401 gcccctgtgg ggtcgctgac gccttccacg ggtacggagt gacgcccgtg atgtagccgg
     2461 tgcgccagag gatcgtgtcg ccgttcatgg atccggtggt ggcgtcgttg atcgcgaacg
     2521 ccgtcgccgc ccaccgcgga tccggtgagc cagggagtcc ctgcacctcg aacgcccagc
     2581 gagtcacacc ctcaggcagt cgggcgccgc cgtggtgggt ctggccccat gccgacgatg
     2641 gcgggccgtc ggtttgaccc atcgacacac cgttgaccca gcccgtgccc tggtccgcaa
     2701 acgcctgatg aaccgacacg gcgagcggcg cgtcggcttc gtcgacctcc tggtcgtaga
     2761 cgaaataccg gtgcgtcgag gcgtcgaaca cgtagccgaa cccgtcgacc cacgggtcgg
     2821 ggtgggagta gccgtcggcg aacaccgggc ggaggaacac ggcatcgtcg aggtcgtcga
     2881 tgtcggcctc ggggctcatc catccgaagt tgcggatctc gccgccgagg aagtacacag
     2941 cgccgggatg gggcgggagg acagcccggt cccactccga cagcacaccc tccatccgga
     3001 gcgaccggac cagcgcggag tgccccttcg atgacgccga ggtctgccgg cgtggccgga
     3061 tcacagcagc gaaccgaagc tcgccgtcga ggtaatagcg gacgattcgc ccacgcgtga
     3121 ggtctgtcgc ctcgacgttg ggcaccatct ccgatagagc ctcgctggtg acgaacaacg
     3181 gaatcgagaa cgccgcagtg ccgaacgctc cgataccagg caactctggg gtgaaagctc
     3241 gggtgcccgt caggtccgtg gtcaggtccc ggacgaacgt cccgtcagcc tcgtacagct
     3301 tcagctggat ccgacgctga ggcggctcga ccgcctccgg gcctggcgtc acgaccctgc
     3361 ctcgggcgcg aagggtgcgg tgaggcggaa ctcgaacatg agcggccacc ccgccggccg
     3421 cttccttccc cgacccgaga gcctgaagcg cgacgcggtg gcggtccgac tctccccgga
     3481 aggcatcgtg agcgtcccgg ttcgtgcgac gacggtcggg tcgaggagtt cggtgcgcca
     3541 gtagttgagg ttcgactcga agccctcgat gacgttcgag tgcgggacgc cctcgtggtc
     3601 gatgtggccg gcgaaccaca tggggatctg gacgatcgtg ggtgcccatc cgacagggtt
     3661 caggatgtag tcaccgaggc cgacctgcgt gtcgtcgccc tcaaggtcca gaccgaacca
     3721 cagtggggtg agggcgtcgt cctcgggcca ccatgggtcc gtcacgatgt ggaacggccc
     3781 cgatgtcccg tcgaacgtga gctgcccgcc gctggtgtca gccacgggag atgtcctgcc
     3841 cggtcgtgac ccacgaatcc gcacgagcga cacggagcgc ctcgttcatg gtcggggcga
     3901 cctgatcggg gcgagcatgg atcgtcggtg cgtagacgac ccgctcgagg ttcgcgacgt
     3961 cctgccgtag tgcggagttc ggcgaggatc tcggcctcgt aggactgtcc gaacgtggtt
     4021 gccgtcggc
//
LOCUS       DAOOMA010000188         4023 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_26972_length_4023_cov_3.251008, whole genome shotgun sequence.
ACCESSION   DAOOMA010000188 DAOOMA010000000
VERSION     DAOOMA010000188.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 4023)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4023)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4023
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26972_length_4023_cov_3.251008"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(84..836)
                     /gene="ccsA"
                     /locus_tag="PLP95_06270"
     CDS             complement(84..836)
                     /gene="ccsA"
                     /locus_tag="PLP95_06270"
                     /inference="COORDINATES: protein motif:HMM:NF013725.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HPB45442.1"
                     /translation="MNDSPQSTSSRGSRILGILAAISALFLVLYAFWFSKADVELGNT
                     VRILYLHVPTVIVAYLCMVANLVASVFYLWKRSEFADQLAAATGEIGMVFLGVTVITG
                     MFWGKPTWGTYWSWGDARLTSTLLLFLLYVGYVVVRNIPAELGQRNVRSAVVGIVAAL
                     MIYPVRMSTEWFSTLHQGRTVGAPGASKISGNQEFALYLGFLTFALFAVWLTMHRFRV
                     GWLADRAAESDLESAIEARRAEGRAEFGKVNP"
     gene            complement(887..1555)
                     /locus_tag="PLP95_06275"
     CDS             complement(887..1555)
                     /locus_tag="PLP95_06275"
                     /inference="COORDINATES: protein motif:HMM:NF015344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015232 - heme transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HPB45443.1"
                     /translation="MLRDAVLVCRKDLLVEWRSRVTTRFVGPFVLSVVMLFAFAFDAD
                     TTMLRAGAGGLFWVTVLFASNTIAQRNAVIDRSDGVHEALRMSSLSPAGVFAGKTASV
                     FVQLSALEVVLAVAMILMYDVRLGGIGLLAASIPLATISVSAIGALYGSLSAGMDGRE
                     ALLPLLSLPALAPVLLAATKCFAVALGTGVGPGWRWVAMLGMLMAIHVVAGMATAVAL
                     LEES"
     gene            complement(1545..2279)
                     /locus_tag="PLP95_06280"
     CDS             complement(1545..2279)
                     /locus_tag="PLP95_06280"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPB45444.1"
                     /translation="MNSEPGIIDPVISLRNVVARAGRFPVLAGIDLDVNPRERVLVQG
                     PNGAGKTSLLRVVAGLLPVSSGVATVLGHDLGAGPGSASSAGPGSEPRGGVNQRAQRR
                     AVRASIGYLAHSTGLYDDLTAADNVRFWAQACRVPASDAEAAMDRVELAQRLRSVPVK
                     RLSTGQRRRVSLAATIVRRPRLWLLDEPHAGLDTADRDLVDGLIVAASEAGATVMFAS
                     HDLDRAVAVSTRIVSLSGGTVVADAA"
     gene            2362..3372
                     /locus_tag="PLP95_06285"
     CDS             2362..3372
                     /locus_tag="PLP95_06285"
                     /inference="COORDINATES: protein motif:HMM:NF016119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004197 - cysteine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0016485 - protein processing [Evidence
                     IEA];
                     GO_process: GO:0045184 - establishment of protein
                     localization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sortase"
                     /protein_id="HPB45445.1"
                     /translation="MISRIFGGVGRSLIFSGTVLLLFVGYQLWGTSLDTQGHQRDLTR
                     EFGETVGVDPDKPKDEALRSIVERLSKIEPRTAEPLEAPAEGEGAGFIEIPKIDLGPT
                     VFVQGVSKADLRKGPGHYTGTPFPGNEGNAGIAGHRTTYSAPFNRIDELVPGDHIIVY
                     TNQGKFTYEVRPSPDERRSGEENRLWGPGWFAVRPSDVSVLDQSDENMLTLTACHPKR
                     SARYRIIVQATLIADPAPAPDEDLDPSTSTVKPENSETVGSDSGDEEAAMIAGDPDEL
                     APAIAWFAAVVAIWLASMAFAAFLRRRQRRAWWIYLFTVALSAWPLWLSFTHVDKFLP
                     SF"
     gene            3496..>4023
                     /locus_tag="PLP95_06290"
     CDS             3496..>4023
                     /locus_tag="PLP95_06290"
                     /inference="COORDINATES: protein motif:HMM:NF025215.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation family protein"
                     /protein_id="HPB45446.1"
                     /translation="MLPRPLNRFAPSARAAATAAAVVLCIVLGAGGCSSNDAPDNSAG
                     EESSESTTTTTTPAGLLTATMDILSGQCFNPVPDPTQQPYAVFVVACDDPHEFEAYST
                     TQLELGEPTPPGTPYPGDLTVANAAEAQCLTGFASFVGLAWESSEYDVQAWWPSAESW
                     TTTNDRSILCGVFRVT"
BASE COUNT          733 a         1423 c         1264 g          603 t
ORIGIN      
        1 gtacccgccg atcaccccga cggtgacggc ccacgagatt cccaccgaaa cccaggccgt
       61 cgtgttgtcg atggcggttg cgatcatggg ttcacctttc cgaattcagc gcggccttcc
      121 gcccgccgcg cctcgatggc cgactccagg tcggactcgg ccgcccgatc ggccagccaa
      181 cccacccgga accgatgcat cgtgagccat acggcaaaca gcgcgaacgt gaggaatccc
      241 agatacagcg cgaattcctg gttccccgag attttcgacg ctcccggggc acccaccgtg
      301 cggccctggt gcaatgtcga gaaccactcg gtcgacatgc gtacgggata gatcatgagc
      361 gccgcgacga tgcccaccac ggcggaacgc acgttgcgct gcccgagctc cgcggggatg
      421 ttccgaacga cgacgtagcc gacgtagagg aggaacaaca gcagggtcga ggtcaggcgg
      481 gcgtcgcccc acgaccagta ggtcccccag gtcggtttac cccagaacat gccggtgatc
      541 accgtgacac ccaggaacac catgccgatc tcgcccgttg ccgccgccag ttgatcggcg
      601 aactctgagc gtttccacag gtagaacacc gacgcgacga ggttcgcgac catgcacagg
      661 tacgcgacga tcacggtcgg aacgtgcagg tacaagatgc gaaccgtgtt gcccaactcg
      721 acgtcggcct tggagaacca gaacgcgtac aggaccagga acagcgcact gatcgccgcg
      781 agaatcccaa gaattcggga cccgcgcgac gaggtggact ggggagaatc gttcatcgaa
      841 agcgttccgt tcacttctgg gccgatgcgc tcgtcggact ccccggtcag gactcttcga
      901 gcagggccac agctgtggcc attccggcca ccacatggat ggccatcagc atgcccaaca
      961 tcgccaccca tcgccagccg ggaccgaccc ccgtgcccaa tgccacggcg aaacacttcg
     1021 tcgccgccaa cagcaccgga gcgagcgccg gcaggctcag cagcggcaac agcgcctcgc
     1081 ggccgtccat cccggccgac agcgagccat agagcgcgcc gatcgccgac accgaaatcg
     1141 tcgccagcgg gatggacgcg gcgagcaggc ctatgccgcc gaggcgcacg tcgtacatga
     1201 ggatcatcgc gaccgcgagc accacctcga gtgcgctcaa ctgcacgaac accgacgcgg
     1261 tcttgccagc gaataccccc gctggcgaca acgaactcat ccgcaacgcc tcgtggacgc
     1321 cgtcggagcg gtcgatgacg gcgttgcgct gggcgatcgt gttggatgcg aacagcaccg
     1381 tcacccagaa cagtccaccg gcgccggcgc gcaacatggt cgtgtcggca tcgaacgcga
     1441 acgcgaacag catcaccacc gatagcacga acggccccac gaatcgcgtg gtgacgcgac
     1501 tgcgccattc gacgagcagg tccttgcgac acaccagcac cgcgtcacgc agcatcggcg
     1561 accaccgtgc ctccgctcaa cgacacgatg cgcgtcgaca ccgcgacggc gcgatcgaga
     1621 tcgtgggacg cgaacatcac cgtcgctccg gcctcgctcg ccgcgacgat caagccatcg
     1681 acaagatccc gatcggcggt atcgaggccg gcgtggggtt cgtccaacaa ccacagccgt
     1741 ggacgccgca cgatcgtcgc cgccaacgaa acgcgtctgc gctgccccgt cgagaggcgt
     1801 ttcacgggga cggacctcag acgttgggcc aactccaccc ggtccatcgc ggcctccgcg
     1861 tcggatgcgg gcacgcgaca cgcttgggcc cagaatcgca cgttgtcggc cgcggtgagg
     1921 tcgtcgtaca ggccggtact gtgcgccagg tacccgatcg aggcccgaac cgcacggcgt
     1981 tgtgcccgct ggttgacccc acctcgtggc tcggagcccg gaccggcact cgaagcggag
     2041 cccggaccgg cgccgaggtc gtgacccagc accgtggcca ctcccgaact caccggcaac
     2101 agccccgcga cgacccgcag caacgacgtt ttgccggccc cgttcggccc ctgaaccaag
     2161 acgcgttctc gcgggttcac gtcgagatcg atcccggcca acaccggaaa acgacccgct
     2221 cgggccacga cgttacgcag tgagatgacg gggtcgatga tgccgggctc gctgttcacg
     2281 caggtctcgt ggtggtcggt cgcaggccaa cgctacccgg gtgcccgcgg gaatcgccaa
     2341 gagtgccggt agcttgacgg cgtgatttct cggatcttcg gtggtgtcgg gcgttccctg
     2401 atcttcagcg gcacggtgct gttgttgttc gtcggctacc aactctgggg cacgagcctc
     2461 gacacccagg gccaccaacg agatctcacc cgggagttcg gcgagaccgt gggggtggat
     2521 cccgacaagc cgaaggacga ggcactgcgc agcatcgtcg aacgactctc caaaatcgag
     2581 ccgcgcaccg ccgagccgct cgaagctccc gcagagggcg agggggcggg attcatcgag
     2641 attccgaaga tcgaccttgg tccgaccgtg ttcgttcagg gggtgtccaa ggcggacctg
     2701 cgcaaggggc ccggccacta caccggtacg ccgtttcccg gcaacgaggg caacgctggc
     2761 atcgccgggc accgcaccac ctacagcgcg ccgttcaacc gcatcgacga actcgtcccg
     2821 ggcgaccaca tcatcgtgta caccaaccag ggcaagttca cctacgaggt tcggccgtcg
     2881 cccgacgaac ggcgaagcgg cgaggagaac cggctgtggg ggcccggttg gtttgcggtg
     2941 cgcccgtccg acgtcagcgt gttggaccag tcggacgaga acatgttgac cctcaccgcc
     3001 tgtcacccga agcgctccgc caggtatcgc atcatcgtcc aggccacact catcgccgat
     3061 ccggcgcccg cacccgacga ggacctcgat ccgagcacgt ccacggtcaa gcccgagaat
     3121 tcagaaacgg tgggctccga ttcgggcgat gaagaggcgg ccatgatcgc cggggatccc
     3181 gacgaactgg cacctgcgat cgcctggttc gcggcggtgg tcgccatctg gctcgcgtcg
     3241 atggcattcg ccgcgttcct gcgccgacgc cagcgccgcg cctggtggat ctacctgttc
     3301 accgtcgcgc tcagcgcctg gccgctgtgg ctctcgttca cccacgtcga caagttcctc
     3361 ccgtcgttct gagcgccggc ccctccggtc gcaccgagcg ccgagcgccg agcgccgagc
     3421 gccgaacacc gagcaccgag cgccgttctc aggccggttc gcgtccgcgg cacgccggtc
     3481 tcgtatcgtt ttggggtgct cccccgccct ttgaatcgct tcgccccgtc cgctcgcgcc
     3541 gcggcgaccg ctgcggcggt cgtgttgtgc atcgtgctgg gcgccggtgg ttgctcgtcg
     3601 aatgacgccc cggacaactc ggcgggcgag gaatcgtccg agtcgaccac gaccacgacc
     3661 acgccggcgg gcctgttgac cgccacgatg gacatcttgt cgggacagtg tttcaacccg
     3721 gtgcccgacc cgacccagca gccctacgcc gtgttcgtcg tcgcgtgcga tgatccccac
     3781 gagttcgagg cctactccac gacacaactc gagctcggcg agcccacccc acccggcacg
     3841 ccctatcccg gtgacctcac ggtggccaac gccgccgagg cgcagtgcct gaccgggttc
     3901 gcgtcgtttg tgggactggc gtgggaaagc agcgagtacg acgtgcaggc gtggtggccc
     3961 tcggccgagt cgtggacgac gaccaatgac cggtcgattc tgtgcggggt ctttcgggtc
     4021 acc
//
LOCUS       DAOOMA010000189         4022 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_26980_length_4022_cov_3.960928, whole genome shotgun sequence.
ACCESSION   DAOOMA010000189 DAOOMA010000000
VERSION     DAOOMA010000189.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 4022)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4022)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4022
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26980_length_4022_cov_3.960928"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..363)
                     /locus_tag="PLP95_06295"
     CDS             complement(<1..363)
                     /locus_tag="PLP95_06295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB45447.1"
                     /translation="MQIVYLSNRPTVLRETWEHVRRHMPFVTRALVIARGNLTGEFGL
                     EDDASVTFVDESEISGRSGAELAALNHVTRNVTLRRAAVDAGLVEDVFVLSDDDYRPI
                     KPIRREFFTDGKRDRSYYF"
     gene            406..1647
                     /locus_tag="PLP95_06300"
     CDS             406..1647
                     /locus_tag="PLP95_06300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020378260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HPB45448.1"
                     /translation="MKLLVICPHYAPDVAPTGEVMTSIASALVERGHRLDIVTALPWY
                     RKHDIEPGWDHKLTQTETTPWGSITRLHPFPTNKSNIWARALAFGGFTGVATLVSMFR
                     RERPDAVLVMSPPLILGIAGWLAARRFRVPMVFNIQDVFPDVAVEVGAITNSKVIAVA
                     RWLERFLYLRSDAVTVLSDDLRDNLRTKIGRRNPDRVRVIPNFVDTERIAPADRMTTY
                     RKEFGLGDRTVVMYAGNVGYSQSLDLLVGAARRFADRRDVVFVVNGEGSGLNEVRGSA
                     DGLDNIVFAGLQPKERLVEVLASADIHTVLLKRGLARSSVPSKMYSILAAGRPCVASV
                     DSDTEVERSIRAANAGVSVPPEDLDAFCEALEPLLDDAEERRDMGASGRRWVENWASP
                     AAVAEAYERLFEELIDRRSRS"
     gene            1725..2564
                     /locus_tag="PLP95_06305"
     CDS             1725..2564
                     /locus_tag="PLP95_06305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB45449.1"
                     /translation="MATPSSSKKIQRVQRAGVNRSVGQRRPLGYPALIAGIIVVGCVL
                     VWLARDARIHGDGERPRAGRDTWYQAYGTYECDGYLANLLPPATESDIEAMGNGVIIV
                     GPSSEETAGRNATLGRFFEAQGMEVTDDSVTFADGTELKAGATCGDGDDASTDTVIKL
                     FVWPPQASDRTKPTVVDADFAWVRFEQNGAAFALALVDKDTDSIDLPGAVDNLSNPDA
                     GAVPRNQATTTTAPASDGDAEGTVQEGSGDAGADEADPGTDADPGADADPGADATTTT
                     AGN"
     gene            2567..3577
                     /locus_tag="PLP95_06310"
     CDS             2567..3577
                     /locus_tag="PLP95_06310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012222871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NDP-sugar synthase"
                     /protein_id="HPB45450.1"
                     /translation="MRAVVLVGGFGTRLRPLTLATPKQMLPIGSVTMFERVIARLGAG
                     GVTEAVVSLGFAPDRFTEAFPDGTCAGVRLHYAIEPEPLDTAGAIRFAARHAGVDETF
                     LVVNGDVLTDLDYRWLVEQHRLLGGEATLHLIGVEDPSRFGVVDTAEDGSVRAFVEKP
                     APGTEPCNLINAGTYVMEPSVLDLIDEGRKVSVERETFPVLVERGTLKAVAYDTYWID
                     AGTPSAYLQACLDLIDGTRSNEAGVAADATVADGAVVEHSMVNPRASIGSGATIRDSI
                     VMAGATIGADAVVEGSIVGAGARIGSGARVIGGAVIGHGETVADAATIDAVTVPDPST
                     WQ"
     gene            3648..>4022
                     /locus_tag="PLP95_06315"
     CDS             3648..>4022
                     /locus_tag="PLP95_06315"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF013530.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HPB45451.1"
                     /translation="MRALVTGGAGFIGSTLVDRLLAEGHSVDVIDSLSSGKLSNLAEA
                     RSNRDHDLTFHQIDIRNAEVVTLIERRKPEVIFHLAAQADVRVSVSDPALDASINILG
                     GINVLEGARRAETRKVVFASSGS"
BASE COUNT          664 a         1320 c         1362 g          676 t
ORIGIN      
        1 gaagtagtac gagcggtcgc gtttgccgtc ggtgaagaac tcgcgtcgga tgggcttgat
       61 cgggcggtag tcgtcgtcgg aaagcacgaa tacgtcctcg acgaggccgg catcgaccgc
      121 cgcacgtcgc agggtcacgt ttcgggtgac atggttgagc gccgcgagtt cggcacccga
      181 gcgaccggag atctcggact cgtcgacgaa cgtcaccgag gcgtcgtcct caaggccgaa
      241 ctcgccggtg agattcccgc gggcgatgac gagtgctcgc gtgacgaagg gcatatggcg
      301 ccgaacgtgt tcccacgttt ctcgcagaac cgtcggccgg ttactcagat agacgatctg
      361 catgggtgcg aagcgtagtg ggtcacgacc tatcatcggc gtgccgtgaa gctgctcgtc
      421 atctgccccc actacgcccc cgatgtcgcc ccaaccggcg aagtcatgac gtcgatcgcg
      481 tcggcgctcg tcgagcgcgg gcatcgcctc gacatcgtga ccgcgctgcc gtggtaccga
      541 aaacacgaca tcgaacccgg ctgggaccac aagctcaccc agaccgagac gacgccgtgg
      601 ggctcgatca cccgcctgca cccattcccg acgaacaaga gcaacatctg ggctcgggcg
      661 ctcgccttcg gcgggttcac cggggtcgcg accctggtgt cgatgtttcg ccgcgaacgc
      721 ccggacgcgg tgttggtgat gtcgcccccg ctgattcttg ggatcgccgg gtggctggcg
      781 gctcgccggt ttcgtgtgcc gatggtgttc aacatccaag atgtgttccc cgacgtcgcg
      841 gtcgaggtgg gagcgatcac gaattcgaag gtgatcgccg tcgccaggtg gctcgagcgg
      901 ttcctgtatc tgcgctccga cgccgtcacc gtgctgagcg acgacctgcg ggacaatctg
      961 cgaaccaaaa tcggtcgccg gaaccccgac cgggtgcggg tgatcccgaa cttcgtcgac
     1021 accgaacgca tcgcccccgc cgaccgcatg accacctacc gcaaggagtt cggcctcgga
     1081 gatcgcaccg tggtcatgta tgcaggcaac gtggggtact cccagagcct ggatctcctc
     1141 gtcggtgctg cacgccgatt cgccgatcgc cgagatgtgg tgttcgtggt caacggggaa
     1201 gggtccgggc tcaacgaggt ccgcgggtcg gcggacggcc tcgacaacat cgtgttcgcc
     1261 ggccttcagc ccaaggaacg cctcgtcgag gtcctggcct ccgccgacat ccacaccgtg
     1321 ctgttgaaac gcggactcgc gcgctcaagc gttccgtcga agatgtactc gatcctcgcc
     1381 gcggggcgcc cctgtgttgc ctcggtcgac tccgacaccg aggttgaacg ttcgattcgt
     1441 gcagccaacg ctggagtatc ggttccccct gaggacctcg atgcgttctg cgaggcgctt
     1501 gagccgctcc tcgacgacgc cgaggagcgg cgtgacatgg gggcgtcggg gcgacggtgg
     1561 gtggagaact gggcgtcgcc cgcggcggtc gcggaggcct acgaacgtct ctttgaggaa
     1621 ctgatcgatc gtcgatcccg cagttgaccc cagcgcggcg cccacgacat cgacgtggtg
     1681 tgaagttcgg ccgccgaggg cgattcgact agctttggag cgctatggct acgccttcct
     1741 cgtccaagaa gatccagcgc gtccaacgcg ccggggtgaa tcgttccgtc gggcagcggc
     1801 gcccgctcgg atatccggcg ctgatcgccg ggatcatcgt ggtcggatgc gttctcgtgt
     1861 ggctcgctcg agatgcgcga attcacggag acggcgagcg acctcgggcg ggtcgcgaca
     1921 cctggtatca ggcgtacggc acttacgagt gcgacgggta cctggcaaac ctcctgccgc
     1981 cggccaccga gtcggacatc gaagccatgg gtaacggcgt gatcatcgtc gggccttcct
     2041 ccgaggagac cgccgggcgc aacgcaacgc tcgggcggtt cttcgaagct caggggatgg
     2101 aggtgaccga cgactcggtc accttcgccg atggcaccga gctcaaggcc ggagccacct
     2161 gcggcgacgg cgacgatgcg agcaccgaca cggtcatcaa gttgttcgtc tggccgccgc
     2221 aggcctcgga tcgcaccaag ccgaccgtgg tcgacgccga cttcgcgtgg gttcgattcg
     2281 agcagaacgg cgccgcgttc gccctcgcgt tggtcgacaa ggacaccgac tcgatcgacc
     2341 tgcccggggc ggtggacaac ctctccaacc cggacgccgg tgcggtgccg cgcaaccagg
     2401 ccacgacgac cactgcgccg gcatctgacg gcgacgccga gggcacggtg caggagggct
     2461 ccggtgacgc cggagcggac gaagccgacc ccggcaccga cgccgacccc ggcgccgacg
     2521 ccgaccccgg cgccgacgca accaccacga ccgccgggaa ctgaccgtgc gcgccgtcgt
     2581 gctcgtcgga gggttcggaa cgcgcctgcg tccgttgacc ctggcgactc cgaagcagat
     2641 gcttccgatc ggttcggtca cgatgttcga gcgtgtcatc gcacggctcg gcgccggtgg
     2701 agtgaccgag gcggtggtgt cgctgggttt cgctcccgac cgtttcaccg aggcattccc
     2761 cgacgggacc tgtgcggggg tgcgcctgca ctatgcgatc gaacccgagc cgctcgacac
     2821 cgccggggcg atccgattcg ccgcccgcca tgcgggggtc gacgagacgt ttctggtcgt
     2881 caacggcgac gtgctcaccg acctcgacta ccgctggctc gtggaacagc accgactcct
     2941 cggtggtgag gcgacgctgc acctcatcgg cgtcgaggat ccgtcgcggt tcggagtggt
     3001 ggacaccgcc gaggacggtt cggtgcgcgc gttcgtcgaa aagccggccc cgggaaccga
     3061 gccgtgcaac ctcatcaacg ccggcaccta tgtgatggag ccgtcggtgc tcgacctgat
     3121 cgacgagggc cgcaaagtgt cggtcgagcg cgagacgttt ccggtgctcg tggaacgagg
     3181 caccctcaag gctgtggcct atgacaccta ctggatcgac gccggcacgc cgagcgccta
     3241 tctccaggcc tgcctcgacc tgatcgacgg cacgcgctcg aacgaagccg gcgtggcggc
     3301 cgacgcaacc gtggccgacg gtgccgtggt cgagcattcg atggtgaatc cacgggcgtc
     3361 gatcgggtcg ggggcaacca ttcgtgactc catcgtcatg gcgggggcca ccatcggtgc
     3421 ggacgccgtg gtcgagggat cgatcgtcgg ggccggagcc agaatcgggt caggggcccg
     3481 cgtgatcggc ggggcggtga tcggccacgg cgagacggtc gccgacgcag cgacgatcga
     3541 tgccgtgacg gtgccggatc cgtcgacctg gcagtagatt tacctcgggc gatcgcactt
     3601 cggcggtcga tccactgcgg cgatgtgccg ttcggaaagg gagtgcaatg cgcgcgttgg
     3661 tgaccggtgg tgcggggttc atcgggtcga ccctcgtcga ccgactgctc gccgagggcc
     3721 actcggtcga cgtcatcgac agtctctcgt cgggcaaact gtcgaatctc gccgaggccc
     3781 ggtcgaatcg cgaccacgac ctcacgtttc accagatcga cattcgtaac gccgaggtcg
     3841 tcaccctgat cgaacgccgc aagcccgagg tgatctttca cctcgccgcg caggccgacg
     3901 tgcgggtctc cgtatcggat cccgcactcg atgcatccat caacattctc ggtggcatca
     3961 acgtgttgga gggcgctcgc cgcgccgaaa cccgtaaggt cgtgtttgcc tcgagcggca
     4021 gc
//
LOCUS       DAOOMA010000190         4017 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_27026_length_4017_cov_3.377082, whole genome shotgun sequence.
ACCESSION   DAOOMA010000190 DAOOMA010000000
VERSION     DAOOMA010000190.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 4017)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4017)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4017
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27026_length_4017_cov_3.377082"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1579)
                     /locus_tag="PLP95_06320"
     CDS             complement(<1..1579)
                     /locus_tag="PLP95_06320"
                     /inference="COORDINATES: protein motif:HMM:TIGR00711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0009410 - response to xenobiotic stimulus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HPB45452.1"
                     /translation="MAHDASPTGAVPADHDPIASGADDTFGIDPGIYARRWQIHSVLC
                     VSLVAIVAAVSSLNVAIPTIIEALEPSPTQTLWIVDSYALVFAGLLLPAGAVGDRFGR
                     KKALQIGIVIFGTMALVASFANSANALIAARAVMGIGAALIMPATLSIITNVFPPHER
                     AKAIAAWAGLSGAGGALGPLLSGLILKADLWWGAVFLINVPIAILLFSLVALRVPESK
                     DPSGHALDPVGVVLSIVTLGSLVFGIIEAPEYGWGSARVLAALGLSLVAGLGFVAYES
                     RTAQPMLDPRLFRFRGFSVGALTITCAFFCMFGTFYALTLYLQFVKGYSPLGAALRTL
                     PQAVVMVLVAPRSPRLVAKIGVHNNVRLGFATLAVAYLIMSRFTVDTPYWVIMICLVL
                     LAVGMAVMTAPTSAVIVGSLPLAKAGVGSAVNDVTREVGGVLGIALMGSFLSTGYASK
                     IVDRLAGSPIPDGPRHAIEESIGVAFGVVDRGLAEGTITNDQAEGIRAIARESFITGN
                     RNAFLAAFGVAVIGGLIV"
     gene            complement(1701..2630)
                     /locus_tag="PLP95_06325"
     CDS             complement(1701..2630)
                     /locus_tag="PLP95_06325"
                     /inference="COORDINATES: protein motif:HMM:NF012321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vWA domain-containing protein"
                     /protein_id="HPB45453.1"
                     /translation="MIDGDEAREAIAQAARRTTARRDLERHDRFSEISPEVGVLDDGA
                     FGDLMDDDLDEALTLLVDMATATDAELRAQARALAARIIVDLAKSGGAARRGIGRLSS
                     RRASVAEGDVDLDASLESISAARAAGEPVSLDELTVRSWVRPDTAVCLLVDRSGSMRG
                     ERLAAAALAAAAVAYRHPHHSAVVAFSGTAVVAKAINEERHGDDLADDVLQLRGHGVT
                     DLGLAARTAQAQLERSKAARRITLLLSDARSTSGGDPTEDAAALRSLGELVILAPSSD
                     TEDAAALAAATGARLIALEGPGDVPRAIAEALG"
     gene            complement(2627..>2906)
                     /locus_tag="PLP95_06330"
     CDS             complement(2627..>2906)
                     /locus_tag="PLP95_06330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB45454.1"
                     /translation="LVLVAAQLAALRNAAAFDPAVSRDAAQLALSGRVRVRDGCSRSA
                     AEIVDELWREHLAPRDAHDPASNEPDGGHDGGPKAHRHPPAVATTRRG"
     assembly_gap    2907..2916
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2917..3566)
                     /locus_tag="PLP95_06335"
     CDS             complement(<2917..3566)
                     /locus_tag="PLP95_06335"
                     /inference="COORDINATES: protein motif:HMM:NF019346.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HPB45455.1"
                     /translation="MTFVDHRGLVDRLDARVVGRRTELEQVIAGLAAGRHLLLEGPPG
                     TGKSTLLRSIAEELDQGFHFVEGNAELTPARLVGTFDPAAVLERGYRAEVFLDGPLTA
                     ALREGSLLYIEEINRIPEETLNVLIGVMSEGSIEVPRLGNVIAGDGFRLVAAMNPFDA
                     IGTARISGAVYDRVCRLEMGYQEVADERAIVEARTSDATDMVGAEFIREIENGEVVV"
     gene            complement(3614..>4017)
                     /locus_tag="PLP95_06340"
     CDS             complement(3614..>4017)
                     /locus_tag="PLP95_06340"
                     /inference="COORDINATES: protein motif:HMM:NF012335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HPB45456.1"
                     /translation="SIEEAMATVPDLTWGDMCDAVILTVGVMTGDLVQPALDLTAKGG
                     TVVMTSVANMMEGEVTLNSFTFAMLNKELKGSLFGSGNPRYDIPELLSMYREGDIKLD
                     ELVTKRYTLDQVNEGYEAMRAGENLRGIIEF"
BASE COUNT          679 a         1290 c         1411 g          627 t
ORIGIN      
        1 cgacgatcag ccctccgatc accgcgacac cgaaggccgc caggaacgcg tttcggtttc
       61 cggtgatgaa cgactcccgg gcgatcgctc ggatcccctc ggcctggtcg ttggtgatcg
      121 ttccctcggc gagcccgcgg tcgaccacgc cgaacgcgac gccgatggat tcttcaattg
      181 cgtgacgcgg gccgtccggg atgggagatc cagcgagccg gtcgacgatc ttcgaggcgt
      241 agccggtgct gaggaacgaa cccatcaagg cgatgcccag aaccccgccg acctcccggg
      301 tgacgtcgtt gaccgcggat cccaccccgg cctttgccag ggggagcgag ccgacgatca
      361 cggcggaggt cggcgcggtc atgaccgcca tgccaaccgc caacaacacg agacagatca
      421 tgatcaccca gtacggcgtg tcgacggtga agcgactcat gatcaggtag gcgacagcga
      481 gcgtggcgaa ccccagacgg acgttgttgt gcacgccgat cttggcgacg agtcgcgggc
      541 tgcggggggc gaccagcacc atcaccaccg cctggggcag cgtgcgcagg gccgctccga
      601 gcggggagta gcccttcacg aactgcaggt agagggtcaa cgcatagaac gtaccgaaca
      661 tgcagaagaa cgcacacgtg atggtgaggg ccccgaccga gaagccgcgg aagcggaaca
      721 gccgcgggtc gagcatcggt tgggcggtgc gtgattcgta ggcgacgaaa cccaaccccg
      781 ctacgaggct gaggccgagc gcggccagca ctcgagctga gccccagccg tactccggcg
      841 cttcgatgat gccgaagacc agcgagccca atgtgacgat gctgaggacg acaccgaccg
      901 ggtcgagcgc atgtccggac gggtctttgg actcggggac gcgcagggcg acgagggaga
      961 acaacaagat ggcgatcggg acgttgatga gaaacaccgc tccccaccac aggtcggcct
     1021 tgaggatcag ccccgacagc agtggtccga gcgcaccacc cgctcccgac agccccgccc
     1081 aggcggcgat ggccttggcg cgttcgtgcg gagggaacac gttggtgatg atcgacaggg
     1141 tcgccggcat gatcaacgcg gcgccgatgc ccatgaccgc tcgagcggcg atcagtgcat
     1201 tggcggagtt ggcgaaggag gccacgagcg ccatggttcc gaagatgacg atgccgatct
     1261 gcagcgcctt cttgcgtccg aagcggtcgc cgacggctcc ggcgggcaac aacagccccg
     1321 cgaagaccag ggcgtaggag tcgacgatcc acagtgtctg ggtgggcgac ggttcgaggg
     1381 cctcgatgat cgtggggatg gcgacgttca gggagctgac tgcggcgacg atcgccacga
     1441 ggctgacgca cagcaccgag tggatctgcc agcggcgtgc gtagatgccg ggatcgatac
     1501 cgaacgtatc gtcggcgccc gacgcgatgg ggtcgtggtc tgccggaacg gctccggtcg
     1561 gggaggcatc gtgggccatg ggtgatgcgg ctttctggcg gggatggacc gaccgaccct
     1621 agatgtggcg tccggggttt cgcaacagtt ctgtactgtt tcgctaccgg accctcttga
     1681 aacgccctgt gcgggagggt tcagccgagg gcctcggcga tggcgcgcgg aacgtcaccc
     1741 ggaccttcca gcgcaatcag gcgggcgccg gtcgctgccg cgagcgctgc ggcatcttcg
     1801 gtgtcgctcg acggtgcgag gatgaccagc tcgccgagcg agcgcaacgc ggcggcatcc
     1861 tcggtcgggt cgccgccgga ggtggagcgg gcatcggaca gcaacaaggt gatgcggcgc
     1921 gccgccttcg aacgctcgag ttgagcctgc gcagttctcg cagcgagtcc gaggtcggtg
     1981 acgccgtggc ctctcagttg caacacgtca tcggccagat cgtcgccatg gcgttcctcg
     2041 ttgatcgcct tggcgacgac ggcggttcca ctgaacgcga cgaccgccga gtggtggggg
     2101 tggcgatagg cgaccgcggc cgcggcgagc gccgcggccg cgagacgctc gccgcgcatc
     2161 gaaccggacc ggtcgacgag taggcacact gcggtgtccg ggcggaccca cgatcgcaca
     2221 gtcaactcgt ccagcgatac cggctccccg gcggcacgtg ccgcggagat cgactcgaga
     2281 ctggcgtcga ggtcgacgtc gccttccgcg accgacgcgc ggcgcgagct caagcgtccg
     2341 attccgcgac gggcggcacc gccggatttc gcgaggtcga cgatgatgcg tgccgccaac
     2401 gcccgtgcct gggcgcgcaa ctcggcgtcg gtggcggtcg ccatgtccac gagcagggtg
     2461 agcgcctcgt cgaggtcgtc gtccatgaga tcgccgaacg ccccgtcgtc gaggacgccg
     2521 acctcggggc tgatctcgct gaagcggtcg tggcgttcga ggtcgcggcg ggcggtcgtg
     2581 cgccgtgccg cctgggcgat ggcttcgcgc gcttcgtcgc cgtcgatcac ccgcgtcgag
     2641 tcgtcgccac cgccggaggg tggcggtgcg cttttgggcc gccgtcgtgt cccccgtccg
     2701 gttcgttcga agccgggtcg tgcgcgtcgc gaggggcgag gtgctcgcgc cagagttcgt
     2761 cgacgatctc ggcagccgaa cgcgagcacc cgtcgcgcac ccgtaccctg cccgacaatg
     2821 ccagctgtgc ggcgtcgcgg gagaccgctg gatcgaaggc ggctgcgttt cgcaacgccg
     2881 cgagttgtgc ggcgacgaga acgaggnnnn nnnnnnacca ccacctcgcc gttctcgatc
     2941 tcacggatga actcggcgcc gaccatgtcg gtggcgtcgc tcgtccgggc ttcgacgatc
     3001 gcccgctcgt cggccacctc ctgatatccc atttcgagcc gacacacccg gtcatagacc
     3061 gctcccgaga tccgggcggt gccgatcgcg tcgaacggat tcattgcggc cacgagacgg
     3121 aacccgtcgc cggcgataac gttgccgagc cgcggcacct cgatcgaccc ctcactcatc
     3181 acgccgatga gtacgttgag ggtctcctcc gggatccggt tgatctcctc gatgtagagc
     3241 aacgagccct ctcggagcgc ggcggtcaac ggtccgtcga ggaacacctc ggcgcggtag
     3301 ccacgctcga ggacggcagc gggatcgaag gtgcccacga gccgcgccgg ggtgagttca
     3361 gcgttgccct cgacaaagtg aaacccctgg tcgagctctt cagcgatgga acgcaaaagc
     3421 gtcgacttgc ccgtgccggg aggtccctcc agaagaagat gccgtccggc cgccagccct
     3481 gcgatcacct gctcgagttc ggtgcggcgg ccgaccactc gagcgtcgag gcgatcgacg
     3541 aggccgcggt ggtcaacgaa ggtcatcgga gggtcgtgag aggtggccga gtgctgctga
     3601 gacgcggagc ggatcagaac tcgatgatgc cgcgcaggtt ctcgccggcg cgcatcgcct
     3661 cgtatccctc gttcacctgg tcgagggtgt agcgcttggt gaccagttcg tcgagcttga
     3721 tgtcgccctc gcgatacatc gacagtagtt cggggatgtc atagcggggg ttgccggaac
     3781 cgaacagact gcccttgagt tccttgttga gcatcgcgaa ggtgaagctg ttgagcgtga
     3841 cctcgccctc catcatgttg gcgacggagg tcatgacgac ggtgccgccc ttggcggtga
     3901 ggtcgagcgc tggctgcacc aggtcgcccg tcatgacgcc gacggtcagg atcacggcgt
     3961 cacacatgtc gccccaggtg aggtcgggga cggttgccat cgcctcctcg atgctgg
//
LOCUS       DAOOMA010000191         4012 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_27069_length_4012_cov_3.094769, whole genome shotgun sequence.
ACCESSION   DAOOMA010000191 DAOOMA010000000
VERSION     DAOOMA010000191.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 4012)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4012)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4012
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27069_length_4012_cov_3.094769"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..109)
                     /locus_tag="PLP95_06345"
     CDS             complement(<1..109)
                     /locus_tag="PLP95_06345"
                     /inference="COORDINATES: protein motif:HMM:NF018158.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB45457.1"
                     /translation="MTGGVASSLGKGLTASSLGRLLKARGLRVTMQKLDP"
     gene            complement(154..978)
                     /locus_tag="PLP95_06350"
     CDS             complement(154..978)
                     /locus_tag="PLP95_06350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB45458.1"
                     /translation="MNGLDWSTSLSDVVVCACAGFIVAMALWAAMQDAFVQQTFLRTN
                     YRGHQLPTAVGVLIVVPYALVVGLSSWLNWDLIDGAALALVLGFGVLGLLDDVGGAGE
                     SGGFRGHLRALAAGRLTTGGLKMLGGPVVALAVTGDLLVAAIVCLAANLANLFDRAPG
                     RTTKVSLLAFGLIALAGWTLPPASVALVIGAAAGLLWGDLNEQYMMGDAGSNVIGAVI
                     GVQIVAAFPSTSARWITLAVLVAFNIASEFVSFSKIIASTPPLRALDLLGARRRPR"
     gene            complement(978..3416)
                     /locus_tag="PLP95_06355"
     CDS             complement(978..3416)
                     /locus_tag="PLP95_06355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB45459.1"
                     /translation="MKVRLETRRTGSSTRWSSRSSTRWSWLNRGSRRNLALVVVLVAV
                     VLASTPSTAGAQVEQDPLDADSSQSGPGRVLIISAPRLTWQSVEQVAPPHLMELFSQS
                     ALASTSVRAAASVTRPADGYLTIGAGNRMAAPASISGGATVEVGELFGDRIPSELFQR
                     TTGVTPTKPILSLDKPQLDRFNEDNYHFGASSGSLAHALNESGRTMSVLGNTDIAMVD
                     SYLRQVGLAAMGRDGQVDDGAVGRQLLRFNAEAAWGLELDHDVYLRVFQRMWAQHDVV
                     LAELSDLERAETARSDSTLEQGAAQYERALRSSDDLVGAMLADVDLDRDTVIVVSPTA
                     PFEELQLTVFAMAGPGIDAGWAKSATTRRARFVTLTDIAPTVLAHFDVPAPSEMNDTR
                     ISAVVASTTPGERIDTMIDDSAEAVLRDRTFGPISVVFVVALVITIVLATLALARVPR
                     LAPLLRGITLVELAFLPTTFLLGLFAIRTATGIGVGLIVGSVGFAFAAALLKRVDSTM
                     PALALVGLLWLVLALDIATGGRLQINTTFGYSPIVAGRFAGFGNQAYSMIAVSALLLA
                     AGFADRRRPDAEGRATVATSAAIGVWMLVTLVLDGHPSMGSDVGGVLAIVPATAVLLL
                     MIRRVKIRARLVAMIGLGTIAVLALFAGLDLGRPPESRTHLGRFASDLFNGEAGQIIQ
                     RKLAANLRVLTSVWAWVIPAALIYFCYLTWRPNRTFSRLMERSPNYRAFGVSALTLGF
                     LSAALNDSGVSLPAIMLAIVSAYTIYLVTELEFSDVREPAMIETVSAESAARVARVAA
                     AEEPSSDHDTAGEQ"
     gene            complement(3413..>4012)
                     /locus_tag="PLP95_06360"
     CDS             complement(3413..>4012)
                     /locus_tag="PLP95_06360"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HPB45460.1"
                     /translation="LPASEAPVPRRERGEVRQGLGLGDDDPLVVCAARLHPQKDLPLL
                     LRAWARLQDRAAWLLIVGDGPQRDELEALRSDLGLDRVLMPGASPHAVDELNAADLVV
                     MSSRWEGAPLVIAEAMQLGKAIVSTRVGVVPDMILGCGEVVEVGDEDALVAAIDRFLA
                     DDELRENCAKSALERGRVLYGAQRLVDEVRGVYVEVVAR"
BASE COUNT          735 a         1414 c         1315 g          548 t
ORIGIN      
        1 aggggtcgag cttctgcatc gtcacccgga ggccacgtgc cttgagcaac cgcccgagcg
       61 aggacgcggt gaggcccttg ccaagtgaac tcgcgacgcc acccgtcacg aagatgtgct
      121 tggtcacacg taactccttt ggtcctcaca aacctagcgc ggccgacggc gtgccccgag
      181 caggtccagg gcgcgcagcg gcggggtcga cgcgatgatc ttcgaaaacg acacgaattc
      241 cgaggcgatg ttgaacgcga cgagcaccgc gagggtgatc catcgggccg aggtcgacgg
      301 gaaggccgcg acgatttgga ctccgatcac cgcgccgatc acgttcgacc cggcatcgcc
      361 catcatgtac tgctcgttga ggtcgcccca cagcaggccg gcggccgcac cgatcacgag
      421 cgccaccgac gccggcggca gggtccaccc ggcgagggcg atcaggccga acgcgagcag
      481 cgacaccttg gtcgtgcggc cgggcgcacg gtcgaacagg ttggcgaggt tcgccgcgag
      541 gcagacgatc gccgccacca gcaggtcgcc ggtcacggcc agggccacca ccgggccacc
      601 cagcatcttg agccccccgg tggtcaggcg gccggccgcc agtgctcgga ggtgaccgcg
      661 gaaccccccg ctctcgcccg ctccgccgac gtcgtcgagc aggccgagca ccccgaatcc
      721 gagcacgagg gcgagcgctg cgccgtcgat caggtcccag ttcaaccacg aactcaaccc
      781 gacgaccaag gcgtagggga ccacgatgag caccccgacg gcggtcggca actggtggcc
      841 ccggtagttc gtccgcagaa acgtctgctg aacaaatgcg tcctgcatgg ccgcccacaa
      901 ggccatggcg acgatgaacc cggcgcacgc acacaccacg acgtccgaca gcgaggtcga
      961 ccagtcgagt ccgttcatca ctgctcccct gccgtgtcgt ggtcggagga tggctcctcg
     1021 gcggcggcca cccgtgccac ccgtgccgcc gattccgctg agaccgtttc gatcatcgct
     1081 ggctcccgaa catcgctgaa ttccagttcc gtcaccagat agatcgtgta ggccgacacg
     1141 atggccaaca tgattgccgg cagcgacacc cccgagtcgt tcagtgccgc cgacagaaac
     1201 ccgagggtga gcgcggagac cccgaaggcg cggtagttcg gcgatcgctc catcaggcgc
     1261 gagaacgtcc ggttcgggcg ccaggtcagg taacagaagt agatgagcgc ggccgggatc
     1321 acccaggccc acacgctggt gagcacgcgc aggttcgctg cgagcttgcg ttggatgatc
     1381 tgccccgcct cgccattgaa gagatccgag gcgaaccggc ccagatgggt ccgcgattcc
     1441 ggtgggcggc cgaggtccaa cccggcgaac agggcgagca cggcgatcgt gccgaggccg
     1501 atcatcgcca cgaggcgggc gcggatcttc acccgtcgaa tcatcaacaa cagcactgcg
     1561 gtcgccggca cgatcgccaa cacgccgccg acatcggagc ccatcgaggg atggccgtcg
     1621 agtacgaggg tgaccagcat ccacaccccg atggccgcac tcgtggcgac ggtcgcacgc
     1681 ccttcggcat ccggtcgtcg tcggtcggcg aacccggccg ccaacaacag cgcgctcacc
     1741 gcgatcatcg aataggcctg gtttccgaac ccggcgaaac gtcccgcgac gatcggcgag
     1801 tagccgaagg tcgtgttgat ctgcagccgg ccaccggtcg cgatgtcgag cgccaacacc
     1861 agccacaaca gccccaccag cgccagggcg ggcatggtgg aatcgacgcg tttgagcagg
     1921 gccgcggcga acgcgaagcc gaccgagccg acgatgagcc ccaccccgat cccggtcgcg
     1981 gtgcggatcg cgaacaaacc gagcagaaag gtcgtcggca ggaaggccaa ctccaccaac
     2041 gtgatgccgc gcaacaacgg ggcgagacga ggcacccgcg ccagcgccag ggtcgccaac
     2101 acgatggtga tcaccagcgc cacgacgaac acgaccgaga tcggcccgaa ggtgcggtca
     2161 cgcagcacgg cctcggcgga gtcgtcgatc atcgtgtcga tgcgttcccc gggcgtcgtc
     2221 gacgcgacga ccgcagagat gcgggtgtcg ttcatctccg acggggcggg aacgtcgaaa
     2281 tgagcgagca cggtcggggc gatgtcggtc agggtcacaa aacgcgctcg tctggtcgtg
     2341 gccgatttgg cccacccggc gtctataccc ggacccgcca tcgcgaacac ggtgagttgc
     2401 aactcctcga acggagccgt cggcgacacc acgatgacgg tgtcgcggtc caggtcgaca
     2461 tcggcgagca tcgcccccac gaggtcgtcg ctactgcgaa gcgcacgctc gtattgggcg
     2521 gccccctgtt cgagggtcga gtcggagcgg gcggtctcgg cccgttcgag gtcggacaac
     2581 tcggccagga ccacgtcgtg ttgggcccac atccgttgga agacccgcag gtagacgtcg
     2641 tggtcgagtt cgagtcccca ggccgcctcg gcgttgaacc gcaacagttg tcggccgacg
     2701 gcgccgtcat cgacctgccc gtcgcgcccc atcgccgcca gaccgacctg acgcaggtag
     2761 gagtcgacca tggcgatatc ggtgttgccc agcacgctca tggtgcggcc ggactcgttg
     2821 agcgcgtggg ccaacgagcc ggaactcgcc ccgaagtggt agttgtcctc gttgaagcgg
     2881 tcgagttgag gcttgtcgag cgacaggatc ggcttcgtgg gcgtgacccc ggtggtgcgc
     2941 tggaacaact ccgagggaat gcggtcgccg aaaagctcgc caacctcgac ggtcgctccc
     3001 cccgaaatcg acgcgggcgc ggccatccgg ttgccggccc cgatcgtgag atagccatcc
     3061 gcgggcctcg tcacactcgc cgcagcccgc actgaggtcg aggcgagcgc tgactgcgaa
     3121 aacagctcca tcagatgcgg cggagcaacc tgttcgacgc tctgccaggt cagccgcggt
     3181 gcgctgatga tcaggacccg accggggccg gactggctgg aatcggcgtc gagggggtcc
     3241 tgttccacct gcgcacccgc cgtcgagggc gtcgatgcca ggaccacggc gacgagcacg
     3301 acgacgagcg cgaggttgcg acgggaaccg cgattcagcc acgaccaccg cgtcgacgac
     3361 cgcgacgacc accgcgtcga cgacccggtc cggcgagtct ccaggcgcac cttcacctcg
     3421 ctacgacctc cacgtacacg ccgcgaacct cgtcgacgag tcgttgggca ccgtagagca
     3481 cccgaccccg ctccagcgcg gacttcgcac aattctcacg cagctcgtca tcggcaagga
     3541 accggtcgat cgccgcgacc agcgcgtcct cgtctcccac ctcgacgacc tcgccgcagc
     3601 cgagaatcat gtcgggcacc acgccgacgc gggtcgacac gatcgccttg ccgagttgca
     3661 tcgcctcggc gatgaccagc ggcgcgccct cccaacgcga gctcatcacc accagatccg
     3721 cggcgttgag ttcgtcgacc gcgtgggggc tcgcccccgg catcaaaacc ctgtcgaggc
     3781 cgagatcgct gcgaagcgcc tcgagttcgt cgcgttgcgg gccgtctccg acgatcagca
     3841 accacgccgc ccgatcctgg agccgggccc aggcgcgcaa cagcagcggc agatcctttt
     3901 gggggtgcag ccgcgccgcg cacaccacca gcgggtcgtc atcgccgagt ccgaggcctt
     3961 gacgcacctc gccacgttcc cggcgcggca cgggcgcctc ggaggccggc ag
//
LOCUS       DAOOMA010000192         3992 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_27266_length_3992_cov_4.159004, whole genome shotgun sequence.
ACCESSION   DAOOMA010000192 DAOOMA010000000
VERSION     DAOOMA010000192.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 3992)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3992)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3992
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27266_length_3992_cov_4.159004"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>3992)
                     /locus_tag="PLP95_06365"
     CDS             complement(<1..>3992)
                     /locus_tag="PLP95_06365"
                     /inference="COORDINATES: protein motif:HMM:NF012321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HPB45461.1"
                     /translation="DRPDRSSEFALVSDGRIPAESLRLFPRGTRYLPVGTSDRNVGIT
                     NVVTSRAGQQLHVQATLTNASRRSVAATARFDVDGHTADTTSVEVPAGGSAEVAATVD
                     DGSRVEVLLVEHDLLRLDDHAYAVAPDEAVVQVAVVGPPSLFLDAVLDASPGLEAVPM
                     TEVEALAPGGLASVDVAVFNQVAVPNDIAVPYLAIAAPAGTGAVTVGPAVENPIATLV
                     RGDTDVMADLDLREFAAAQAQTLNAPAATTLVGDEHTPLIVSGRDGDVAFVYLGFTLE
                     ASNFGLLPAFPVFVDRALATLGQTQLVSGSLRPGDRLPVTGAGEGTLVSPSGTEIPVV
                     AGSATPVLDRTGFWTVRFEGQPERLVVVNPAPSETSLAPRADLGLPGGRPLAEADSVP
                     VLRSLKTWLGLAAIAAIAAEWWFSRRRRGVTRSQWRWAERARLAVGALLVLGLVLPSI
                     PLPSRRVAVVVVADVSASLGSSTDAVESVVAEVFGARGRNDVAAVVAVDANAHIDASL
                     STDWKKFSTTRDPQATDLAGGLRVAGALVPTDRASRIVVISDGRPTTGDATSEIDRLR
                     DADIPVDVVTVEPMTPEDLAVVSVDVASRVRTGDRVDVAVSIEATTPQQTSVELRAND
                     EIVGTKVVDAPAGRSEVRFDVAVPEATSIRWRATVAGPANGRVENDRAGASTRIEGVP
                     NVLVVEGFEGSGSAISAALGAAGNNVSVLAATALSSISQLATVDIVVLADVAAKDFSR
                     AQMTIVDQAVRELGVGLLAVGGTNSFSAADYLGSDLEALLPVTSEAKDPKRRSRLAQV
                     FAVDTSGSMGACHCADDADGDNSRLEGGVRKTDIAHEAIELALGEFRPDDQAGVLGLD
                     SSFRWLAPLAPVGDAADLGKAIGGLAPDSAGSGTKLGSALDEAAAALRNSDTGLRHVV
                     LFTDGFVDQGQFTKLEADAAALREEGITVSVFGTGEGAARQLQSIATAGGGRFYAGRD
                     LKELPDLLLEETKVVARQLIVEGEFVPERTSNAAIVSRLDAAPVLRGYLATTERPTAT
                     VHLRIGEDRDPLLATWQVGLGRTAAWTSDGGTRWAAGWSGWDGAVTFWSDLVRSLAPQ
                     PAGEVSMRFGERETMLVATFGDDVPDGATVNATVTSPDGTAQVVTMRRIDQRTFEAEA
                     PTGPAGSYTAAVTAEVRGSAVGSMSAVAERGFSDEFLARSADRDWMQTLSSSTGGRGE
                     IAASAAFERTGTRKGERDLDLRWMMLLLGLIGWIAAIAIGRLRFRSGALAIVRASDRA
                     KAVPGLDAVGARRARAAKDRASAIEGSAERAQATSAPVPPSQPPPPASETPPPASEAS
                     SGPSSLDSLL"
BASE COUNT          630 a         1391 c         1416 g          555 t
ORIGIN      
        1 ccaacagtga gtcgagcgaa ctcggcccgg acgatgcctc cgaggctggt ggcggtgtct
       61 ccgaggctgg tggcggtggc tgggatggcg gaaccggcgc cgacgtagcc tgagcgcgct
      121 cggccgagcc ttcaatcgcg ctcgcccggt cctttgctgc gcgtgcccgc cgcgctccga
      181 cggcgtcgag acccgggacg gccttggcgc ggtccgaggc ccgcacgatg gcgagcgcgc
      241 cgctgcggaa ccgcagacga ccgatggcga tggccgcgat ccagccgatg agccccagca
      301 ggagcatcat ccatcgcagg tcgaggtcgc gttcaccctt gcgggtcccg gtgcgttcga
      361 acgccgccga ggcggcgatc tcgccgcgtc cacccgtcga gctcgacagc gtctgcatcc
      421 agtcgcgatc ggcggagcgc gccaggaatt cgtcggagaa tccccgctcc gcaacggcgc
      481 tcatcgagcc gacggccgat ccgcgcacct cggcggtcac cgcggcggta tagctgccag
      541 caggtccggt gggggcctcg gcctcgaagg tgcgttggtc gatccgccgc atcgtgacca
      601 cctgggccgt gccgtcgggg gaggtgacgg tcgcgttcac cgtcgccccg tcgggcacat
      661 cgtcgccaaa ggtggcgacc agcatggtct cacgttcgcc gaaacgcatc gacacctcgc
      721 cggccggctg cggagcgagc gaacggacca ggtcgctcca gaacgtcacc gcaccgtccc
      781 aaccgctcca cccggccgcc cagcgcgttc cgccgtcgct ggtccacgcc gcggtgcgcc
      841 cgagcccgac ctgccaggtc gccagcagcg gatcgcggtc ctcgccgatg cgcaggtgca
      901 ccgtcgcggt cggacgttcg gtcgtcgcga ggtacccccg caggacgggt gcggcgtcga
      961 ggcgtgacac gatcgccgcg ttcgaggtgc gctcgggaac gaattcgcct tcgacgatca
     1021 gttggcgggc gacgaccttg gtctcctcca acagcagatc cggcaactcc ttgaggtcac
     1081 gacctgcgta gaagcgtccg ccacccgccg tcgcgatcga ctggagctgg cgtgccgcgc
     1141 cctccccggt gccgaacacc gacacggtga tgccctcctc gcgcagcgcc gcggcatcgg
     1201 cctcgagctt ggtgaattgg ccctggtcga cgaagccgtc ggtgaacaac acgacgtgac
     1261 gcagccccgt gtcggagttg cggagcgccg cggcggcctc gtccagcgcg ctgcccaact
     1321 tcgtccccga tccggccgaa tcgggggcca gtccgccgat cgccttgccg aggtcggccg
     1381 catcgccgac gggagcgagg ggagcgagcc aacgaaacga cgagtcgagc cccaagacgc
     1441 cggcctggtc gtcggggcgg aactcgccaa gggccaactc gatggcttcg tgggcgatgt
     1501 cggtcttgcg gacgccaccc tcaagacgcg agttgtcgcc gtcagcgtcg tcggcacagt
     1561 ggcaggcccc catcgatccg gaggtgtcga cggcgaacac ctgggcgagg cgtgaacgac
     1621 gtttggggtc cttcgcctcg ctggtcaccg gtaacaacgc ttcgagatcg ctgcccaggt
     1681 agtcggcggc ggaaaacgag ttggtcccgc cgaccgccaa caacccgacg ccgagttccc
     1741 gtaccgcctg gtcgacgatg gtcatttgag cgcggctgaa atccttcgcg gcgacatcgg
     1801 ccaacacgac gatatcgacg gtggcgagtt gcgagatcga actcaacgca gtcgccgcca
     1861 gcaccgacac gttgttgccc gccgcgccca atgccgccga aatcgcggaa ccggagccct
     1921 cgaaaccctc gacgaccaac acattcggca cgccttcgat ccgggtcgac gccccggccc
     1981 ggtcgttttc gacgcgaccg ttggccggcc ccgccaccgt ggcccgccag cgaatcgacg
     2041 tggcctcggg caccgcgaca tcgaagcgca cctcgcttcg cccggcgggg gcatcgacga
     2101 ccttggtgcc cacgatctcg tcgttcgcac gcaactccac cgacgtctgc tgtggcgtgg
     2161 tcgcctcgat cgaaacggcc acgtcgaccc ggtcaccggt gcggactcgc gaggcaacgt
     2221 cgaccgacac cacggcgagg tcctcggggg tcatcggttc gacggtgacg acatcgacgg
     2281 ggatgtcggc gtcgcggagc cgatcgattt ccgacgtcgc gtcaccggtc gtggggcggc
     2341 cgtcggagat gacgacgatg cgcgaagccc gatcggtggg aacgagcgcg ccggcgaccc
     2401 gcaacccgcc cgccaggtcg gtggcctgtg ggtcgcgggt cgtggagaac ttcttccagt
     2461 ccgtcgacag cgacgcatcg atgtgggcgt tggcgtcgac cgccaccacc gcggcgacat
     2521 cgttgcggcc acgtgcacca aacacctcgg cgacgaccga ctcgacggcg tcggtgctcg
     2581 agccgagtga ggccgacaca tccgcgacga cgaccacggc gacgcgtcgg ctcggcaacg
     2641 gaatcgaggg caacacgagc ccgaggacca gcaaggcacc taccgcgagg cgagcacgct
     2701 cggcccatcg ccactgcgat ctggtcacgc cgcgacggcg gcgcgagaac caccactcgg
     2761 cagcgatggc cgcgatggcc gcgagaccga gccaggtctt gagcgagcgc agaaccggca
     2821 cggagtcggc ctcggcgagc ggccgcccgc ccggaagccc gaggtcggcg cgcggcgcga
     2881 gggaggtttc gctcggtgcg gggttgacca cgacgagccg ttcgggttgt ccctcgaaac
     2941 gcacggtcca gaatccggtg cggtcgagca ccggggtggc agagccggcg acaaccggga
     3001 tttcggttcc cgatggggag accagcgtgc cctcacccgc gccggtgacg ggcagccggt
     3061 cgcccgggcg tagcgatccg gagaccaatt gcgtctggcc gagggtcgcg agggctcggt
     3121 cgacgaacac gggaaaggcg ggcaggaggc cgaaattcga ggcttcgagt gtgaagccga
     3181 ggtagacgaa ggcgacgtcg ccgtcgcggc ccgacacgat gagcggggtg tgctcgtcgc
     3241 cgaccagggt cgtcgcggcg ggtgcgttca gcgtctgcgc ctgagcggcg gcgaactcgc
     3301 gaaggtcgag atcggccatc acgtcggtgt cgccacgcac cagcgtggcg atcgggttct
     3361 cgacggcagg cccgaccgtg accgccccgg tgcccgccgg cgcggcgatc gccaggtacg
     3421 gcaccgcgat gtcgttgggt acggccacct ggttgaatac cgccacgtcg accgacgcga
     3481 gtccgccggg cgcgagcgcc tcgacctcgg tcatcggcac cgcttcgagc ccgggcgatg
     3541 cgtcgagcac tgcgtcgagg aacagcgacg gcgggccgac gaccgcgacc tggacgaccg
     3601 cttcgtcggg agcgaccgca taggcgtggt cgtcgagtcg caagaggtcg tgttcgacga
     3661 gcaggacctc gacgcgtgag ccatcgtcga cggtggcggc gacctcggca ctgcccccgg
     3721 ccggcacctc gaccgaggtg gtgtcggcgg tgtgcccgtc gacgtcgaat cgtgcggtgg
     3781 cggccaccga ccgccgcgac gcgttcgtca gggtcgcctg cacgtggagt tgctgtccgg
     3841 cccggctcgt cacgacattg gtgatgccga cgttgcggtc cgaggtgccg acgggcagat
     3901 agcgcgtgcc ccgagggaag agccgcaacg attcggcggg gattcgaccg tcggagacaa
     3961 gcgcgaactc gctcgaccgg tcgggtcggt cg
//
LOCUS       DAOOMA010000193         3975 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_27416_length_3975_cov_3.741071, whole genome shotgun sequence.
ACCESSION   DAOOMA010000193 DAOOMA010000000
VERSION     DAOOMA010000193.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 3975)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3975)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3975
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27416_length_3975_cov_3.741071"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..875)
                     /locus_tag="PLP95_06370"
     CDS             complement(<1..875)
                     /locus_tag="PLP95_06370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease N-terminal domain-containing
                     protein"
                     /protein_id="HPB45462.1"
                     /translation="MAFDPNTWTLKTQEAVNAAVERASAANNPEVTPEHLLAALLSQD
                     DGIVLPIVRKIGLNPLSLRNGVDERLAKLPTAYGSDTRVSKALTSLIESGDAERVDLG
                     DDYLSTEHLLLSFVRADESKPDADKLLGGTNREQVLAALAEVRGSHRVTSQNPEDQYQ
                     SLEKYGRDLTDAARRGKLDPVIGRDDEIRRVVQVLSRRTKNNPVLIGEPGVGKTAVVE
                     GLAQRIVEGDVPETLRNKRLIALDLASMVAGAKYRGEFEERLKAVLKEITDSDGEIIT
                     FIDEMHTVVGAGGGS"
     gene            complement(878..1261)
                     /locus_tag="PLP95_06375"
     CDS             complement(878..1261)
                     /locus_tag="PLP95_06375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012224844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HPB45463.1"
                     /translation="MPEFHQAVYVISVAAELAGVHPQTLRIYERKGLLRPARTGGGSR
                     RYSEADIEDLRRIQQLTSEGLNLAGVTKVLELERRIAHLERELKRTTEDAATALDAIA
                     RQRRSELVPIPSAAVVLYDQTRRRR"
     gene            complement(1280..2413)
                     /gene="dnaJ"
                     /locus_tag="PLP95_06380"
     CDS             complement(1280..2413)
                     /gene="dnaJ"
                     /locus_tag="PLP95_06380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015799342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HPB45464.1"
                     /translation="MTVQREWFEKDYYAALGVSPTASQKDITTAYRRLARQFHPDANP
                     NDESAEDRFKEVSAAYDVVGDAERRAEYDQVRSMGTSMGSSMGGSGGFGRPGEPGFSD
                     ADISDLLGNLFGRGQSSAWSGSGPFSRGPQRGHDLEADLYLSFLDSIRGVTTSVNLVS
                     DVTCGDCHGNGAEAGTTPRSCPDCGGRGVIEDNQGFFAMSRPCGLCNGRGSIIDTPCA
                     TCRGTGAVSRPRAVRVKLPRGVRDDQQVRLKGKGGPGRGGGPAGDLYIRVHVEPDPVY
                     SRDGDDLIVRVPVTFAELALGADIKVPTIDGEPVTIRIPAGTPNGRQFRVAGRGIESA
                     SGPGALLVAVDLSVPTQMSALERAALEKYRDLSTQNPREHLGV"
     gene            complement(2430..2987)
                     /locus_tag="PLP95_06385"
     CDS             complement(2430..2987)
                     /locus_tag="PLP95_06385"
                     /inference="COORDINATES: protein motif:HMM:NF013212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - chaperone binding [Evidence
                     IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HPB45465.1"
                     /translation="MMSDDQFSAEASEEVGAVEEAADTFSVGGAEEAQPSLEDVFVDV
                     VDDLESVTAQRDEYLDLARRVQADFENYKRRVANQELETRARAAEALVTELLPVLDAC
                     DAALDHGAEEVAPIQSALIAALERKGLGTIAAAGEDFNPELHEAVLSEPGEGGETGPV
                     VVEVMRSGYQWNGRVVRPAMVKVRG"
     gene            complement(2984..>3975)
                     /locus_tag="PLP95_06390"
     CDS             complement(2984..>3975)
                     /locus_tag="PLP95_06390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012224851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HPB45466.1"
                     /translation="EITSDLLDRCKIPFEQAIKDAGLTVGQLDHVILVGGSTRMPAVQ
                     ELVQSVSGREPHKGVNPDEVVAMGAAVQAGVLKGDVKDVLLLDVTPLSLGIETRGGVM
                     TKLIERNTTIPTRRTEVFTTAEDMQAQVEIHVLQGEREMSRYNKTLGTFQLTDLPPAP
                     RGVPQIEVTFDIDANGIVNVSAKDRATSKEQSITITGQSTLSKDQIDQMVKDAEAHAE
                     EDAKRREEAEELNKADSLVYQTEKFLRDNDDKVPADQKARVDEAIAASKEAIASGDLD
                     RVRSTTEALTTASQELSQSLYEAASTEGADGFGSAGAADDDVVDAEIIDDEAQ"
BASE COUNT          622 a         1349 c         1242 g          762 t
ORIGIN      
        1 tcagagccgc cgccggcgcc gacgacggtg tgcatctcgt cgatgaacgt gatgatctcc
       61 ccatcggagt cggtgatctc cttgaggacg gctttgagcc gttcctcgaa ttcgccgcgg
      121 tacttcgctc cggcgaccat cgacgccagg tcgagagcga tgagacgctt gttgcgcagc
      181 gtctcgggca cgtcgccttc cacgatgcgt tgagcgagcc cttcgacgac ggcggtcttg
      241 cccacccctg gctctccgat gagcaccggg ttgttcttgg ttcggcggct caacacctga
      301 accacgcgcc gaatctcgtc gtcgcgcccg atcaccgggt cgagcttgcc gcgccgggcc
      361 gcatcggtca ggtcgcggcc gtacttctcg agcgactggt actgatcctc ggggttctgc
      421 gacgtcaccc gatggctgcc tcgaacttcc gccaacgccg caagcacctg ctcgcgattc
      481 gtcccaccca gcaacttgtc ggcgtcgggc ttggactcgt cggcgcgcac gaacgacaac
      541 agcaggtgtt cggtcgacaa gtaatcatcg ccaaggtcga cgcgttcggc gtcgccggac
      601 tcgatgagcg aggtcagcgc cttcgacacc cgggtgtcgg aaccgtatgc cgtcggcaat
      661 ttggcgaggc gttcgtcgac gccgttgcgc aacgacagcg ggttcagccc gatcttgcgc
      721 acgatcggca acacaatgcc gtcgtcctga gacagcaacg ctgcgagaag gtgctcggga
      781 gtgacctccg ggttgttcgc cgcgctggcg cgttcgaccg ccgcgttcac ggcttcttgg
      841 gtcttgagag tccaggtgtt cggatcgaat gccatggtta ccgcctcctg cgggtttggt
      901 catagagcac gacggcggcc gacggaatgg gaacgagctc ggatcgacgt tgacgcgcga
      961 tggcgtcgag cgccgtcgcc gcgtcctcgg tggtccgctt cagttcccgc tcgagatggg
     1021 cgatacgccg ctcgagttcg agcaccttcg tgaccccggc gaggttcagt ccctcgctgg
     1081 tgagttgttg gatgcggcgg aggtcctcga tatcagcttc gctgtatcga cggcttcctc
     1141 cgccggttcg cgcagggcgc aacaggccct tgcgttcgta gatgcgcagt gtctgcggat
     1201 gcactccggc cagttccgcc gccaccgaga tgacgtacac cgcctggtga aactcgggca
     1261 tcgcctacct ccttgtcatc tacaccccga gatgttcccg gggattctgg gtggagagat
     1321 cgcggtactt ctccagcgcc gcccgctcga gcgccgacat ctgtgtcggg accgacaagt
     1381 cgacggcgac aagaagtgcg cccggtcccg aagcggactc gatgccgcgt cctgccacac
     1441 ggaattggcg tccgttcggg gtacctgccg ggatccggat cgtgaccggt tcgccatcga
     1501 tcgtcggcac tttgatgtcg gccccgagcg ccaactcggc aaaagtgacc ggcacccgca
     1561 cgatgaggtc atccccatct cgcgagtaga ccgggtccgg ttcgacgtga acccgaatgt
     1621 acaggtcgcc tgccgggcca ccgccacggc cgggcccgcc cttgcctttg agccgtacct
     1681 gctgatcgtc gcgcacccca cgaggaagct tcacccgcac agcccgaggg cggctcaccg
     1741 caccggtgcc acgacatgtt gcacatgggg tgtcgataat cgacccccgg ccgttgcaga
     1801 ggccgcacgg acgactcatg gcgaagaacc cctggttgtc ttcgatgacg ccgcgaccgc
     1861 cacaatccgg acaggaacgt ggagtggtcc ccgcttcggc cccgttgccg tgacagtcac
     1921 cgcaggtgac atcgctgacc aggttgaccg aggtggtcac ccctcggatc gagtcgagga
     1981 acgacaggta cagatcggcc tcaaggtcgt gcccgcgctg cggtccacgc gagaagggtc
     2041 ccgatccgga ccaagcgctc gactgcccgc ggccgaacag gttgccgagc agatcgctga
     2101 tgtcggcgtc gctgaacccg ggttcgcccg ggcggccgaa gccgcccgag ccgcccatgg
     2161 atgaccccat cgacgtgccc atcgaccgca cctggtcgta ttcggcgcgc cgctcggcgt
     2221 cgccgacgac gtcgtaggcc gccgagacct ccttgaaccg gtcctcggcc gactcatcgt
     2281 ttggattcgc gtcgggatgg aactggcgcg ccaggcgtcg gtaggcggtg gtgatgtctt
     2341 tttgcgacgc cgtgggggac accccgagcg ctgcgtagta gtccttttcg aaccattcgc
     2401 gttgcacggt catcgcgaca cctcctctcc tagccgcgaa ccttgaccat cgccgggcgc
     2461 acgacccggc cgttccactg ataacccgac ctcatcacct cgacgacaac cggcccggtt
     2521 tctccaccct cgccgggctc cgagagcacc gcttcgtgga gctcaggatt gaagtcctca
     2581 cccgctgccg cgatggttcc gaggcccttg cgttcgaggg cggcgatgag tgccgattga
     2641 atcggcgcta cttcctcggc gccatggtcg agtgccgcgt cacatgcatc gagcaccggg
     2701 agcagttccg tcaccagcgc ctcggcggca cgggctcgcg tctccaactc ctgattcgca
     2761 acacgccgct tgtaattctc gaagtcggcc tggacccggc gtgcgaggtc gagatattcg
     2821 tcgcgttgag cggtgaccga ctcgaggtca tcgaccacgt cgacgaacac gtcttcgagt
     2881 gacggctggg cctcctcggc accgcccaca ctgaaggtgt cggccgcctc ctcgacggcc
     2941 cccacctcct cggacgcttc agcgctgaac tgatcgtcgc tcatcattgc gcctcgtcat
     3001 cgatgatctc ggcgtcgacc acgtcgtcat cggccgcccc ggcggaaccg aacccgtcgg
     3061 cgccttcggt ggaagccgcc tcatagagcg attggctgag ctcctggctg gcggtcgtca
     3121 acgcttcggt cgtcgacctc acccgatcga gatcgccaga cgcgatggct tccttggacg
     3181 ccgcgatcgc ctcgtcgact ctcgccttct gatcggcggg gaccttgtcg tcgttgtcgc
     3241 ggaggaactt ctcggtctga tacacgagcg agtcggcctt gttcagttcc tcagcctcct
     3301 cgcgacgctt cgcgtcctcc tcggcgtgag cttcggcatc cttgaccatc tggtcgatct
     3361 gatccttcga cagcgtcgac tgaccggtga tcgtgatcga ctgctccttg ctggtcgcgc
     3421 ggtccttggc ggagacgttc acgatgccgt tggcgtcgat gtcgaaggtc acctcgatct
     3481 gcggcacgcc acgcggcgcc gggggcagat cggtcagctg gaacgtgccg agcgtcttgt
     3541 tgtaccgaga catttcgcgc tcgccttgca gcacgtgaat ctcgacctgc gcctgcatgt
     3601 cctcggccgt ggtgaacacc tcggtgcgac gcgtcgggat ggtggtgttg cgctcgatga
     3661 gcttcgtcat caccccgcca cgggtttcga tgccgagcga cagcggggtc acgtcgagca
     3721 acaggacgtc tttgacgtcg cccttgagca cgccggcctg aaccgccgca cccatggcga
     3781 ccacctcgtc ggggttgaca cccttgtgcg gctcacggcc gctcaccgac tgcaccaatt
     3841 cctgaaccgc cggcattcgg gttgagcctc cgacgaggat cacgtgatcg agctgaccga
     3901 cggtgagccc ggcgtccttg atcgcctgtt cgaaggggat cttgcaacga tcgagcagat
     3961 cggaggtgat ctcct
//
LOCUS       DAOOMA010000194         3972 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_27441_length_3972_cov_3.512127, whole genome shotgun sequence.
ACCESSION   DAOOMA010000194 DAOOMA010000000
VERSION     DAOOMA010000194.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 3972)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3972)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3972
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27441_length_3972_cov_3.512127"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..220
                     /locus_tag="PLP95_06395"
     CDS             <1..220
                     /locus_tag="PLP95_06395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB45467.1"
                     /translation="LAVMLLLLVSVNAVAAFVVGFLPMFACGISLERSLRHLAWASLD
                     QIGSALAPAERSTHGDSADRFPAEPEDD"
     gene            complement(262..2589)
                     /locus_tag="PLP95_06400"
     CDS             complement(262..2589)
                     /locus_tag="PLP95_06400"
                     /inference="COORDINATES: protein motif:HMM:NF023418.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3488 and DUF4129 domain-containing
                     transglutaminase family protein"
                     /protein_id="HPB45468.1"
                     /translation="MIRSDAPATTPDCRRQRTAPYGIDDALVVLAVVALSFATAFCAA
                     RAFTPMSDVMGRLAITIVTCASLVVVARSARLPAATTFACSLPAGVLAIGVIFDIPYR
                     TMGLVPTAESIAWLHESMTDSFSGFLQAVPPVSATPGFVIPIAAVLWFCTSFGIFAAV
                     DSHGPLHAAAAPLTLFATTSILLLGRYAIASTAAMALALMLLRLATAARSVSIRHSPR
                     RSGSSALTRGIWVSGTAVILTSVAAALVVNAVVPLNVLGAIDLRAIGRPEPPRTVESP
                     LVSVSALLRSQSTRRLFDVDASSAHYWRLTALDRFDGISWSATADYESVGDGRLAGEA
                     PANAAADTVRQRFTLREAFSDWLPAAFAPTRIDLDESLRFDRDSTSIFLPTDDRPAAI
                     SYEVTSSVPDRDAVTADRFASITTTPDDTTDTIHNLIDPSVAELARVITEGRSPFESA
                     LALERFFHTDRRFTYDTNVDYSRQGDPLVAFLNDGAGFCQQFATAFAAMARAVGIPAR
                     VAIGFVPGTATPIDESSTLFAVRANDGHAWPELYFDGLGWVPFEPTPGAQNPDASSYS
                     TAPEPTGSGRDRDDLADSTTTTAAPATTIADSAVAPTTTSDPSNPSDASDGDDASDGL
                     DDRSAWRPGRGSALVALIALVAGATIVARVITVRRRARHLQAPDRPADRRIEASWDRV
                     VAELSRIGIVAQRSETPLEFAERAHREVGEQEPLDTAIDLRRLGEIESRRRYAGTPPH
                     PDDADRVAEIADQVHDLVWARLASADKVRFLLDLR"
     gene            complement(2586..3917)
                     /locus_tag="PLP95_06405"
     CDS             complement(2586..3917)
                     /locus_tag="PLP95_06405"
                     /inference="COORDINATES: protein motif:HMM:NF013998.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HPB45469.1"
                     /translation="MARSRAATLRPTGAGWATIGFCGFALAVGRATGITEFLFGGLFG
                     GIVVVLCAVVAKVSARSVEIDRALSRDRVPMASPLRSIFTVSTTGRFPWPPVVMVDHI
                     DGEAQPSRLLADPESRSGSGARTRTERVEIPMHAATRGLVRFGPVQLVHRDPLALMAA
                     TTTHPAPATLLVLPTITPLGTMSPAIGAQPDAQLRSGARRMANRNDEFEALREYVEGD
                     DIRLVHWAASARVGQLVVREFEHRSLHRTLVVVDTGVGMGLGTGVGTGDSNGPTSDDV
                     DSAFEQAVSNAASVLVDLVDAGEATTFIVGAQVGVSPVRFDIDSPSDLDRALDTLATV
                     ARDAVPASSSNWIEGPIGRAHGVTGGDQVVVCTAGLDRDGVAALSAAASTGVDVIVLD
                     ASRSGASRSGASTSGAMRWFAATDADQLRDAWTAAGGAVGVHAAASRAGRR"
BASE COUNT          641 a         1276 c         1479 g          576 t
ORIGIN      
        1 gctggcggtg atgttgctgt tgttggtgag cgtcaacgcc gttgccgcgt tcgtcgtcgg
       61 gttcctgccg atgttcgcat gtggcatctc gctcgaacgc agcctgcgcc acctcgcctg
      121 ggcgtcgctc gaccagatcg gctcggcgtt ggctccggcg gagcgctcca cccacggcga
      181 ttccgcggac cggttccccg ccgaacccga agacgactga cctgaaaaga cgacggacct
      241 gatcagcttt gctcgatccg ctcaccgtag atcgagcagg aaccggacct tgtcggcgga
      301 ggcgaggcgc gcccacacga ggtcgtggac ctgatcggcg atctcggcga cgcggtcggc
      361 gtcgtcgggg tgcggcggcg tgccggcata gcgacgacgg ctctcgatct ctccgagccg
      421 gcgaaggtcg atcgcggtgt cgagcggttc ctgttctccc acctcgcggt gggcccgttc
      481 ggcgaattcg aggggggtct cggagcgctg cgcgacgatg ccgatgcgcg acagctcggc
      541 gacgacccgg tcccacgacg cctcgatccg tcggtcggcg gggcggtcgg gcgcctgtag
      601 gtgccgcgcc cgtcggcgaa cggtgatcac ccgggcgacg atcgtcgcgc ccgcgacgag
      661 ggcgatgagc gcgacgagtg cgctgccccg gccgggtcgc cacgcggagc ggtcgtcgag
      721 cccgtccgaa gcgtcgtcgc catccgacgc atcggatggg ttggacggat cggaggtcgt
      781 ggtcggcgca acggcggaat cggcgatggt ggtcgccggg gcagcagtcg tcgtcgtcga
      841 gtctgcgagg tcgtcgcgat cgcggcccga tccggtcggc tcaggtgcgg tggaatagct
      901 cgaagcatcg gggttctgcg cgcccggggt tggctcgaac ggcacccagc cgagcccgtc
      961 gaagtacaac tcgggccacg catggccgtc gttggcccga acggcgaaca gcgtcgagga
     1021 ctcgtcgatc ggggtggcgg tgccggggac gaatccgatc gcgacccgtg cggggattcc
     1081 caccgcccgc gccatcgcgg cgaacgcggt ggcgaattgt tggcagaacc cggccccgtc
     1141 gttcaagaag gccaccaacg ggtcgccctg gcgcgagtaa tcgacgttcg tgtcataggt
     1201 gaagcggcgg tcggtgtgga agaaccgctc gagggccagg gccgactcga acggggagcg
     1261 gccttcggtg atcaccctcg ccaactcggc cacgctgggg tcgatcaggt tgtggatcgt
     1321 gtcggtcgtg tcgtccgggg tcgtggtgat ggacgcgaat cggtcggcgg tgaccgcgtc
     1381 gcgatccgga accgaggagg tgacctcgta ggaaatcgct gcgggacggt cgtcggtggg
     1441 caggaagatc gaggtcgagt cccgatcgaa gcggagagat tcgtcgagat cgatgcgggt
     1501 cggggcgaat gctgcgggga gccagtcgct gaaggcttcg cggagggtga agcgttgtct
     1561 caccgtgtcg gccgctgcgt tggcgggggc ctccccggcg aggcggccgt ccccgacgga
     1621 ttcgtaatcg gcagtggctg accacgagat gccgtcgaac cgatcgagcg cggtcagtcg
     1681 ccagtagtgc gcactcgacg cgtcgacgtc gaagagccgt cgcgtcgact ggctgcgcag
     1741 tagcgcagag acgctgacca acgggctctc gacggtgcgc ggtggttcgg gccgaccgat
     1801 cgcccggagg tcgatcgccc ccaacacgtt caggggaacg acggcgttca cgacgagtgc
     1861 cgcagcgacc gaggtgagaa tcaccgcggt tccgctcacc cagatcccac gcgtgagcgc
     1921 ggaggaaccg gagcgccgcg gcgagtgtcg aatcgacacc gaccgggcgg ccgtcgcgag
     1981 ccgtaacaac atcagggcca gcgccatcgc cgcggtggac gcgatcgcat accggcccag
     2041 gagcaggatg gaggtggtcg cgaacaaggt gagcggcgct gcggctgcgt gaaggggccc
     2101 gtgggagtcg acggcggcga agattccaaa cgaggtgcag aaccacagga ccgcggcgat
     2161 cgggatcacg aatcccggag tggcgctcac cgggggcacc gcctggagga atccgctgaa
     2221 ggagtcggtc atggactcgt gaagccacgc gatcgactcg gcggtgggca cgagacccat
     2281 cgtccggtac gggatgtcga agatgacgcc gatggcgagg accccggccg gtagggaaca
     2341 cgcgaaggtc gtcgccgccg gcagtcgagc ggatcgggcg acgacgacga ggctggcgca
     2401 ggtgacgatg gtgatcgcca gacgtcccat gacatcgctc atgggagtga aggctcgagc
     2461 ggcgcagaac gcggtcgcga acgacaatgc gaccacggcg agcaccacga gggcgtcgtc
     2521 gatgccgtag ggcgcggtcc gctgtcgcct gcagtcgggc gtcgtggccg gcgcgtcgga
     2581 gcggatcatc gtcggccggc ccgtgaggcc gcagcgtgga ccccgaccgc cccacccgcg
     2641 gccgtccatg cgtcgcggag ttggtcggcg tcggtcgcgg caaaccagcg catcgcgccg
     2701 ctggtggagg cgccggatcg cgaggcgccg gatcgcgagg cgtccagcac gatgacgtcg
     2761 actccggtgg acgccgcagc gctcaatgcg gccacgccat cgcggtcgag accggcggta
     2821 cacaccacca cctggtcgcc gccggtgacg ccatgggcgc ggccgatggg gccttcgatc
     2881 caattcgacg acgaggccgg aacagcgtcg cgagccaccg tcgccagggt gtcgagtgct
     2941 cgatcgagat cgctcggcga gtcgatgtcg aatcgcacag ggctgacgcc gacctgagcc
     3001 ccgacgatga aggtggtggc ctcgccggca tcgaccaggt cgaccagcac cgacgcagcg
     3061 ttcgacaccg cctgttcgaa ggccgaatcg acgtcgtcgc tcgtgggtcc gttcgaatcg
     3121 cccgtaccca ctcccgtacc cagtcccata cccactcccg tgtcgaccac gacgagggtt
     3181 cggtgcagcg accggtgttc gaattcccgc acgacgagct gaccgacccg cgcgctcgcc
     3241 gcccaatgaa ccagtcggat gtcgtcgccc tcaacgtatt cgcgcagcgc ttcgaactcg
     3301 tcgttgcggt tcgccatgcg ccgcgcgccg ctgcgcagtt gggcgtctgg ttgcgccccg
     3361 atggccgggc tcatcgttcc gagcggggtg atggtcggca ggacgaggag tgtcgcaggc
     3421 gccgggtgtg tcgtcgttgc ggccatgagt gcgagcgggt cgcggtgcac gagttgtacc
     3481 ggcccgaaac gcacgaggcc gcgggtagca gcgtgcatcg ggatctcgac gcgttcggtt
     3541 cgcgtgcgcg ccccgctgcc ggatcgcgac tccgggtcgg ccaggagccg ggacggctgc
     3601 gcctcgccgt cgatgtggtc gaccatgacg acgggcggcc agggaaagcg accggtcgtc
     3661 gagacggtga agatcgaccg caacggtgag gccatgggaa cccggtcccg cgacagcgcc
     3721 cgatcgatct ccaccgatcg ggcgctgacc ttcgcgacga ctgcgcagag cacgacgacg
     3781 atgccgccga acagcccgcc gaagaggaat tcggtaatgc cggtggcgcg ccccaccgcg
     3841 agtgcgaacc cgcagaaccc gatcgtcgcc cagccggcac cggtcgggcg aagggttgcg
     3901 gcgcggctac gcgccatggg caccgagggg aaccgacgcg gacctcggaa tgggaacggt
     3961 tgcgaggagg tc
//
LOCUS       DAOOMA010000195         3966 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_27498_length_3966_cov_4.510867, whole genome shotgun sequence.
ACCESSION   DAOOMA010000195 DAOOMA010000000
VERSION     DAOOMA010000195.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 3966)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3966)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3966
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27498_length_3966_cov_4.510867"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..584
                     /locus_tag="PLP95_06410"
     CDS             <1..584
                     /locus_tag="PLP95_06410"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB45470.1"
                     /translation="AAGLAVRGLRASRFGRVLIAQRDNERAAMSFGTGVVAAKLAAFG
                     ISGALAALGGLLLTLQQGAFRADLFDVNAGVAVFVAAVVGGLASPLGAVLGAVYLRGA
                     QWVLPGNWQMLASSAGVLMVLLMVPDGLVGVWTRARNQLIAALIGRPVDERDRVEPSD
                     DPTPSRLSRAEWPDGFHHEAPESVFVAPEGVIA"
     gene            581..3631
                     /locus_tag="PLP95_06415"
     CDS             581..3631
                     /locus_tag="PLP95_06415"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HPB45471.1"
                     /translation="MSAAALRSSREAMAPYVEGMFSPRRWWATLRHPVESLRQVAGDG
                     PAFALVVLFGLNAVDELDRTGFGILLPTIRDHFNMSDTGILSLVALTTLGALLLQLPI
                     AILADRGSRVRLAIAGGIAWGVFSFATGMATTVWALVLIRSGAGIGRAVVDPTHSALL
                     SDYFAIERRPAVFSFHRAANAVGQFVGPLTAGVLAHYFSWRVPFFVFALPTALFVVLA
                     LRLREPIRGAHERAAAGESAEAIATEEPDASFGEAWRTVWKIESLRRIWYAIPFLAVS
                     IIGFVSLAGLLYERVYGYDDLQRGVVAALVEPMQLIGLAIGGRVGVRLVMRDPALIFR
                     FLRKVAFVSGAWAALFAWSPNIVVTIIANIGLTSCLAILLPGVLSALSLAIPARARSV
                     GFSVASYWAIPGLAFVPLIGWLSDNVGIRWGMLVITPILVVGGVMISSVATSINRDIA
                     DVWNSSAARSRALIERREGRSKLLVVDHLNVSYDNVQILFDVSFGVDEGDVVALLGTN
                     GAGKSTLLKAISGIAEADYGAVIFDGRDITHAPPHEIATFGVGQMPGGHGVFAPLTVA
                     ENLRAAAWLHRRDRVATRAAIDQALAAFPSLANRLESPAGDLSGGQQQMLALAMNLIG
                     APKLLMIDELSLGLAPVVVEQLAKLVRQVAAEGTTIILVEQSVNVALTLADTAFFMEK
                     GEIHFSGPTAELLERPDLLRSVFLGKAANAATSRTATRPVVHQTAGHHLRHPLAPPVL
                     ETMDLSVSFGGVRAVDAVNLTVNEREIVGVIGANGAGKTTLFDLICGYLNADGGRVML
                     GDWDVTRVPPHERARAGLGRSFQDAALFRTLTVAETIAVSLERWVAVTDPISAMMRTP
                     ALYDSEKAVAARVDELIELFDLGSLRHRFIGELSTGSRRVVDLACAVAHSPAVLLLDE
                     PSSGIAQRESEALGPLVRRLRDELGCAIVVVEHDMVLLASIADRLVALESGAVIASGD
                     PAVVLADERVVTSYLGSSPAAIARSGVVASSTHRGASFDEGNTP"
     gene            3628..>3966
                     /locus_tag="PLP95_06420"
     CDS             3628..>3966
                     /locus_tag="PLP95_06420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB45472.1"
                     /translation="MTEPPEDAASEPTSAPMPAAALRPSAPPPANPLRKFGPIWGVAL
                     LLVAVLVAIVVTSGSEDETSTPASSTTGSSTTHEAEPVSLREGVDMYSVAEAEGRADD
                     IDWGDRCDTDI"
BASE COUNT          529 a         1277 c         1417 g          743 t
ORIGIN      
        1 tggcggcggg tttggcggtg cgggggctgc gggcgagcag attcggccgc gtgctcatcg
       61 cccagcgcga caacgaacgc gccgcgatgt ccttcggcac gggtgtggtg gcggcgaagc
      121 tcgccgcgtt cgggatctcg ggggcgttgg ccgcgctcgg cggattgttg ttgacgctgc
      181 aacagggggc gtttcgagcg gacctgttcg acgtcaacgc cggggtggcg gtgttcgtcg
      241 cggcggtggt cggcggcctg gcgagtccgc tcggagcggt gctgggcgcg gtctacctgc
      301 gcggcgccca atgggtgctg cccggcaact ggcagatgct tgcgtcgtcg gcgggggtgc
      361 tgatggtgct gttgatggtg cccgacggcc tcgtcggggt gtggacgcgt gcccgcaacc
      421 agctcatcgc ggcgctgatc ggcaggccgg tcgacgagcg cgatcgcgtc gaaccgagcg
      481 atgatccgac cccgtcgcgc ctgtcgcgag ccgagtggcc cgacgggttc caccacgagg
      541 cgccggagtc ggtcttcgtc gcacccgaag gggtgatcgc gtgagcgccg ctgcacttcg
      601 ctccagccgc gaggcgatgg ccccctacgt cgaagggatg ttcagcccga ggcgttggtg
      661 ggcgacgctg cgtcacccgg tcgagtcgct gcgacaggtc gccggcgacg gcccggcgtt
      721 cgcactcgtc gtgttgttcg gactcaatgc ggtggacgaa ctcgaccgca cggggttcgg
      781 catcttgttg ccgacgattc gcgaccactt caacatgtcc gacaccggca tcttgtcgct
      841 cgtggcattg acgacgctgg gagcgctgct gttgcagttg ccgatcgcca tcctggccga
      901 tcgcggcagt cgggttcgtc tcgccatcgc cggcgggatc gcctgggggg tgttctcgtt
      961 cgccaccggg atggccacga cggtgtgggc gctggtgctg atccgatcgg gtgctggcat
     1021 cggccgggcg gtggtcgacc cgacgcattc ggccctgctg tcggattact tcgcgatcga
     1081 gcgacgcccg gcggtgtttt cgtttcaccg tgcggccaac gcggtcggcc aattcgtcgg
     1141 gccactcacc gccggggtgt tggcccacta cttcagctgg cgggttccgt tcttcgtgtt
     1201 cgcccttccg acagcgctgt tcgtcgtgtt ggcgctgcgc ctacgggagc cgatccgcgg
     1261 tgcgcacgag cgggctgcgg cgggggagag cgcggaggcg atcgccaccg aggaacccga
     1321 cgccagtttc ggtgaggcgt ggcgcacggt gtggaagatc gagtcactgc ggcggatctg
     1381 gtatgccatc ccgtttctcg ccgtgtcgat catcgggttc gtctcactcg ccgggctgct
     1441 ctacgaacgg gtctacggct acgacgacct gcaacgagga gtcgtcgccg cgctcgtcga
     1501 accgatgcag ctcatcgggc tcgcgatcgg tggccgggtc ggcgtgcgcc tcgtcatgcg
     1561 cgaccccgcc ctgattttcc ggttcctgcg caaggtcgcg ttcgtgagcg gggcctgggc
     1621 cgcgttgttc gcctggtcgc ccaacatcgt cgtcacgatc atcgcgaaca tcggcttgac
     1681 ctcgtgtctg gcgattctgc tgccgggcgt gctgtcggcg ttgtcgttgg cgattcctgc
     1741 gagggcacgg tcggtcgggt tttccgttgc gtcgtactgg gcgattccgg gactcgcctt
     1801 tgtgccgctg atcggatggc tgtccgacaa cgtcgggatc cgctggggaa tgctggtgat
     1861 caccccgatc ctggtggttg gcggggtcat gatctcgagc gtcgccacct ccatcaaccg
     1921 cgacatcgcg gacgtgtgga actcgagcgc cgcccgaagt cgcgccctga tcgaacggcg
     1981 cgaagggcgt tccaagctgc tggtcgtcga tcatctcaac gtgtcctacg acaacgtgca
     2041 gatcctgttc gacgtctcct tcggcgtcga tgagggagac gtggtggcgt tgttgggaac
     2101 caacggtgcc ggcaagtcga cattgctcaa ggcgatctcg ggcatcgccg aggccgacta
     2161 cggcgcggtc atcttcgatg gccgcgacat cacccacgct ccaccccatg agatcgccac
     2221 gttcggtgtg ggccagatgc cgggcggaca tggggtgttt gccccgttga ccgttgcgga
     2281 gaacctgcgc gccgcggcct ggctgcaccg gcgcgaccgc gtcgcgacac gggccgcgat
     2341 cgatcaggcg ctggcagcgt ttccctcgct ggcgaacagg ctggagtcgc cggcaggcga
     2401 cttgtcgggt ggccaacagc agatgctcgc gttggcgatg aacctgatcg gggccccgaa
     2461 gctgttgatg atcgacgagc tgtcgctcgg cctcgccccg gtggtggtgg agcagttggc
     2521 gaagctcgtt cgtcaggtgg ctgcggaggg aacgacgatc atcctcgtcg agcaatcggt
     2581 caacgttgca ctcaccctcg cggacacggc gttcttcatg gagaagggcg agatccactt
     2641 ctcgggtccg accgccgagc tgctcgagcg tcccgacctg ttgcgttcgg tgttcctcgg
     2701 caaggcggcc aacgccgcga ccagccggac cgcgacacgc cctgtcgtgc accaaaccgc
     2761 cggccaccat ctccgccacc ccctcgctcc accggtgctc gaaacgatgg atctgtcggt
     2821 gtccttcggc ggcgtccgcg ccgtcgacgc ggtgaatctc accgtgaatg aacgcgagat
     2881 cgtcggggtg atcggggcga acggagcggg taagaccacc ctgttcgatc tgatctgcgg
     2941 gtacctcaac gccgacggtg gacgggtcat gttgggcgac tgggacgtca cccgggttcc
     3001 acctcacgaa cgggcgcgtg ccgggctcgg acgttcgttc caggacgcgg cgctgtttcg
     3061 cacgctcacc gtggccgaga ccatcgcggt gtccctcgaa cgttgggtgg cggtcaccga
     3121 tccgatcagt gccatgatgc ggacccctgc gctgtacgac tcggagaagg cggtggcggc
     3181 gcgggtggac gagttgatcg agctgttcga cctcgggtcg ttgcggcatc gattcatcgg
     3241 cgaactctcg accggttcga ggcgggtcgt cgacctggcc tgcgcggtgg cccactcgcc
     3301 ggcggtgttg ctgctggacg aaccctccag cgggatcgct cagcgtgaat cggaggccct
     3361 cggcccgctc gttcgccggc tgcgcgacga gttggggtgc gccatcgtcg tggtcgaaca
     3421 tgacatggtg ctgctggcct cgatcgccga ccgcctcgtg gccctcgaat ccggcgcggt
     3481 gatcgcgtcg ggcgacccgg cggtggtgct cgcggacgaa cgcgtcgtca cctcctacct
     3541 cggctcgtcc ccagcggcga tcgcccgctc cggggtcgtc gcgtcgtcga cgcaccgagg
     3601 agcatcgttt gatgaaggga acaccccatg actgaaccgc ccgaggacgc tgcctccgag
     3661 ccgacgtccg ccccgatgcc cgcggcggcg ctgcggccga gcgcaccgcc gccggccaac
     3721 cccctgcgca agttcgggcc gatctggggg gtcgccctgt tgttggtcgc ggtgctggtg
     3781 gccattgtcg tcacgtcggg ttccgaggac gagacgtcga cccctgcgtc gtcgacgacc
     3841 ggttcttcga cgacgcacga ggctgaaccg gtgtcgcttc gcgagggggt cgacatgtac
     3901 tcggtcgccg aggccgaagg acgggccgac gacatcgact ggggggatcg ctgcgacacc
     3961 gatatc
//
LOCUS       DAOOMA010000196         3934 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_27812_length_3934_cov_3.259087, whole genome shotgun sequence.
ACCESSION   DAOOMA010000196 DAOOMA010000000
VERSION     DAOOMA010000196.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 3934)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3934)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3934
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27812_length_3934_cov_3.259087"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..768)
                     /gene="npdG"
                     /locus_tag="PLP95_06425"
     CDS             complement(<1..768)
                     /gene="npdG"
                     /locus_tag="PLP95_06425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020379088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent F420 reductase"
                     /protein_id="HPB45473.1"
                     /translation="MYGKESHFGWRNPTTVRQDAPMKIGVLGGTGPAGSGLAARLASV
                     GFEAIIGSRSKYRALEVRDNLIEKWADYDLPLFAGDNSEAASADVVVVATPWDAAAST
                     AKEVAEELRGKVVISMANAIAKVGHEFQPLVPPRGSVAASVQAAVPQAMVAGALHHVP
                     AKELGNLRHPCISDVLVCSDHPEATEVTIDIVSKLPGMRPLDSGELSNAAPIEAFVSV
                     MLQLNVKYKTRVALQLTGLEKHFATFETAELKTPASAS"
     gene            complement(784..2256)
                     /locus_tag="PLP95_06430"
     CDS             complement(784..2256)
                     /locus_tag="PLP95_06430"
                     /inference="COORDINATES: protein motif:HMM:NF013775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HPB45474.1"
                     /translation="MSRTGTVPNEPQRPPEPQRPASLAETVAEALEATLGTEVHHLVR
                     LSAGASRETWAFDTDRPLILQRRATAGATSGGTTSGGTSAGEQDSSITTSVAQEADLL
                     RAARAAGVAVPEVVASGDGVDEPLGAPWMICARVPGESLPRTLLRDERFAGVRAGLAR
                     RCGEAIGAIHRIDPEPFVGRLSEEDQIASLRRVLDTVSDVSAVFELVLRWLELNPAPR
                     RDRVVVHGDFRTGNLLVDHDDSDAAGLTAVLDWELTHLGNPVEDLGWFCVRAWRFGSP
                     HRAGGFGPVEDLLAGYRAVSGEDVTSQELAWWELLGTVKWGVICAMQAHYHLSGATRS
                     VELAAIGRRIAENEHDALNLLAELVGGELAGQAPDEVSHLSDDRARSLYGRPTAAELL
                     EGVREFLADDVLAATEGRVRFHTRVAINVLAIVERELAAGEAPAGRHAERLAVLGVGS
                     DEELAEAIRAGAFDERLLAVASQLRGSVEDRLSVANPGYR"
     gene            complement(2228..3301)
                     /locus_tag="PLP95_06435"
     CDS             complement(2228..3301)
                     /locus_tag="PLP95_06435"
                     /inference="COORDINATES: protein motif:HMM:NF012780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HPB45475.1"
                     /translation="MDVAAVPSMLVAAMLVASVLVAASVGARRALLSTVEARLDDPDR
                     RRTRRSDATTRVGVGDAAGVRTRSSTRLWAAPARSVLAAQVVVVGAGVGCGIAIGTTS
                     GWSEAVLVMVVMGVSAAAGDAAARRSPARVLDLAAPIVALASGVVAPLTALSSALSSA
                     LSSALSGVGPADRVERAESGPESCLEPSLEASLEASLEGGLLDEEDHAMLVNALAFEQ
                     RRTEEVMVNVAEIVGVDRSTSVAEAEQVVMNSGHSRLLVRGPTATRHDEVLGFVHAKD
                     LMAVPTSRREHPISFGIIRMTLRANSDASLGDVLAQMRRSRRHVALIDDGAGELVGMV
                     TMEDILEEIVGDIVDESDRHRAE"
     gene            complement(3363..>3934)
                     /locus_tag="PLP95_06440"
     CDS             complement(3363..>3934)
                     /locus_tag="PLP95_06440"
                     /inference="COORDINATES: protein motif:HMM:NF015436.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transporter associated domain-containing
                     protein"
                     /protein_id="HPB45476.1"
                     /translation="TGLSRVIVTGGDLDDIVGQVHIKNLLAVAEPQRAAALLGDLAVE
                     VMMVPASLRLVELLREMRVAASPIAVVLDEYGGTAGLVTVEDVVEELVGEIDDEHDRA
                     GARRHPVQFGGTSVLPGGLNLAEVEELVGLCLPHGRYETLGGFVMERVGAVPEVGDRF
                     EFAGYRFEVLDMLRRRVMSVRVIEPEGQR"
BASE COUNT          669 a         1453 c         1238 g          574 t
ORIGIN      
        1 gctcgccgac gccggcgtct tgagctccgc cgtctcgaag gtggcgaagt gcttttcgag
       61 cccggtgagc tgcagcgcga cacgggtctt gtacttgacg ttgagctgca gcatcaccga
      121 cacgaaggcc tcgatcggcg ccgcgttcga cagctcgccc gaatcgagcg gacgcatgcc
      181 ggggagcttc gacacgatgt cgatggtcac ctcggtggcc tcgggatgat ccgaacacac
      241 cagcacgtcg gaaatgcagg ggtggcgcag gttgccgagt tccttggccg ggacgtggtg
      301 cagcgcaccg gcgaccatgg cctggggaac cgcggcctgc acagacgccg cgaccgaacc
      361 gcgcggtggc accaggggtt gaaactcgtg gccgaccttg gcgatggcat tggccatcga
      421 gatcacgacc ttgccgcgaa gctcctcggc gacctccttg gccgtcgatg cggcggcgtc
      481 ccacggcgtg gccacgacga cgacatccgc gctggccgcc tcggagttgt ccccagcgaa
      541 caacggcagg tcgtagtccg cccacttttc gatgaggttg tcacgcacct cgagcgcacg
      601 gtatttcgac cgggagccga tgatcgcctc gaatccgacc gacgccagcc gcgcggcgag
      661 accgcttccg gcaggacctg ttccacccaa tactccgatt ttcatcggtg catcttggcg
      721 aaccgtggtc gggttgcgcc aaccaaagtg ggattccttg ccgtacatca gcgtctcctc
      781 ccctcaccgg tagcccgggt tggcaaccga aagccggtcc tcgaccgagc cgcgcaattg
      841 cgacgctacc gccagcaggc gctcgtcgaa cgccccggcg cgtatcgctt cggcgagttc
      901 ctcatcgctg cccaccccaa ggacggcgag gcgttccgca tggcgcccgg ccggtgcctc
      961 gcccgccgcc aactcgcgct cgacgatcgc cagcacattg atcgccaccc gggtgtggaa
     1021 ccgcactcgg ccctcggtcg cggccagaac gtcgtcggcc aggaactcgc gaacgccctc
     1081 gagcagttcc gccgcggttg gacgcccgta gaggctccgg gcccgatcgt cgctcaagtg
     1141 gctgacctcg tccggtgctt ggccggcgag ttcgccgccg acgagctccg cgagcaggtt
     1201 caacgcatcg tgttcgttct cggcgatgcg ccgcccgatg gccgccaact cgaccgaacg
     1261 ggtcgctccc gacaggtgat agtgcgcctg catcgcacag atcacccccc acttcacggt
     1321 tccgagaagc tcccaccacg ccaactcctg cgacgtcacg tcctcgccac tcacggcgcg
     1381 gtagccggcg agcaggtcct cgaccggacc gaacccgccc gcccgatggg gcgatccgaa
     1441 tcgccaggcc cgcacgcaga accagccgag gtcctccacg gggttgccga gatgggtcag
     1501 ttcccaatcg agcaccgcgg tgagccccgc agcgtcggag tcgtcgtggt cgaccaggag
     1561 gttgccggtt cgaaaatcgc cgtgcaccac gacccgatcg cgccgtggcg ccgggttcag
     1621 ttcgagccag cgcaacacga gttcgaagac cgcagagaca tcgctcaccg tatcgaggac
     1681 ccggcgcaac gaagcgatct ggtcctcctc gctcagccgg ccgacgaagg gctccgggtc
     1741 gatccggtgg atcgctccga tggcctcgcc gcaacgccgt gcgagaccgg cgcgcacccc
     1801 ggcgaatcgt tcgtctcgca gcagcgtccg gggcagcgac tcccccggca cgcgcgcaca
     1861 gatcatccac ggcgctccga ggggctcatc gacgccgtcc ccgctggcca caacctcggg
     1921 caccgcgacc cccgccgctc gggctgcccg caacaggtcg gcctcctggg cgaccgatgt
     1981 ggtgatcgac gagtcctgct ccccggccga cgtaccgccc gaggtcgtac cgcctgatgt
     2041 cgcgccggcg gtcgcacgtc gctgcaggat cagcggtcga tcggtgtcga aggcccaggt
     2101 ctcgcgactc gcccccgccg agaggcgaac caagtggtgg acctccgttc cgagggtcgc
     2161 ttcgagcgct tcggccacgg tctcagcgag cgacgctggc cgctgtggtt caggtggacg
     2221 ctgtggttca ttcggcacgg tgccggtccg actcatcgac gatgtctccc acgatctctt
     2281 cgagaatgtc ctccatcgtg accataccga cgagttcgcc ggcgccgtcg tcgatcaacg
     2341 caacgtggcg ccgagatcgt cgcatctgcg ccaacacgtc gccgagggag gcgtcgctat
     2401 tggcccgcaa cgtcatgcgg atgatgccga acgagatcgg atgctcacgc cgggaggtcg
     2461 ggacggccat gagatccttc gcgtgtacga acccgaggac ctcatcgtgg cgcgtcgccg
     2521 tcgggcctcg gaccaacagg cgactatggc ccgagttcat caccacctgc tcggcctccg
     2581 ccaccgaggt cgaacgatcg actccgacga tctcggcgac gttcaccatc acctcctcgg
     2641 tccggcgctg ttcgaaggcc aacgcgttca ccaacatggc gtggtcctcc tcatcgagca
     2701 ggccaccctc gagggaggct tccagcgaag cctccaacga gggctcgagg caggactcgg
     2761 ggccggactc ggcccgctca accctgtcgg cgggtccgac cccggacagc gccgaagaca
     2821 aggctgagga caacgccgaa gacagagcgg tcaacggtgc cacgactccc gaggcaaggg
     2881 ccacgatggg tgcagcgagg tccagcaccc gcgccggcga tcgccgcgcc gccgcgtccc
     2941 cggccgcggc actcacgccc atcacgacca tgacgaggac cgcttcggac cagccgctgg
     3001 tggtaccgat ggcgatccca caaccgactc ccgcgccaac gacgacaacc tgggcggcga
     3061 gaacgcttcg ggctggggcg gcccacaggc gagtcgatga gcgagtgcgg actcccgccg
     3121 catcgccgac accgaccctc gtggtcgcat cggagcggcg tgtgcgacgt cgatccgggt
     3181 cgtcgagtcg cgcctcaacc gtcgacagga gcgcccgacg agccccgacc gacgctgcaa
     3241 caagcaccga tgcaacaagc atcgctgcaa caagcatcga cggtacggca gcgacgtcca
     3301 cgaccgccgc catcaccacc gaccaccgcc ctgacgacgc cggtcaggcc cgtgtgcgcc
     3361 ggtcaacgct gcccctcggg ttcgatcacc cgcaccgaca tcacccgccg acgcaacatg
     3421 tcgaggacct cgaagcggta cccggcgaac tcgaaccggt cgccgacttc gggcaccgcc
     3481 cccacgcgct ccatgacgaa ccctcccagg gtctcgtagc gcccatgcgg taggcatagc
     3541 ccgacgagct cctcgacctc ggcgaggttg agacctcccg gcaggaccga ggtgccgccg
     3601 aactgcacgg ggtggcggcg cgcaccggca cggtcgtgtt catcgtcgat ctcgccgacg
     3661 agttcctcca ccacgtcttc cacggtgacc agaccggccg tgcccccgta ctcgtcgagc
     3721 accaccgcta tcggcgacgc cgccacgcgc atctcgcgga gcagctcgac gagacgcaac
     3781 gaggcgggca ccatcatcac ctccaccgcg agatcgccca gaagcgccgc agcgcgctgc
     3841 ggctcggcga ccgccaacag gttcttgatg tggacctgtc ccacgatgtc gtcgaggtcg
     3901 ccgccggtca cgatcacccg gctcagaccg gtcc
//
LOCUS       DAOOMA010000197         3932 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_27826_length_3932_cov_4.402373, whole genome shotgun sequence.
ACCESSION   DAOOMA010000197 DAOOMA010000000
VERSION     DAOOMA010000197.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 3932)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3932)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3932
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27826_length_3932_cov_4.402373"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1308)
                     /locus_tag="PLP95_06445"
     CDS             complement(<1..1308)
                     /locus_tag="PLP95_06445"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF019334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HPB45477.1"
                     /translation="MVRRLLERGDLFENRYRIVYFLGSGASAQVYLARDLKLDRDVAI
                     KVLHEFVAADTRFLRRFRAEAKAVAALDHRHIVKLYDYNESAANGLPYITMEYLGGGS
                     LRSVLDHGPLTVAQVIGIGIAAASALDHAGRRGIVHRDIKPANLLFGLDRQLKIADFG
                     VARALSESSVTDLAGSPGTPKYVAPEQVQGSLVSPAADVYALALVLAEAVSGEAVFER
                     DTPDATSLARLGHDLSAPAEAGPLGPLLEAAGKLDPEERISAVDLQLALIELSRQFAR
                     PADIPIASAVPPSELDAVAADVAAEVAAIDLTAVLEVEDPTEVLSAVGATGAPALGEP
                     VSGEPASGDSDPDTDIATPATVRLFDAAAIDDTALDTAALDTAAVAEIAIGADGTSPP
                     DESVDVAAGEAAGESAATGALDAGPDADSDAPPVDPSNERSPCR"
     gene            1394..1831
                     /locus_tag="PLP95_06450"
     CDS             1394..1831
                     /locus_tag="PLP95_06450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB45478.1"
                     /translation="MPKKVKALLYVLSACAIALGVWVYNNAVTGPDQTSLATKPYVDR
                     LIPTSGSQAPPQATVGIDLEPGYDAFLVVNGEKIANTISEADQDPDGLRKAPTLGLIE
                     YTPGLGKRVERLATPRTCVDAWVWKVVDGPDNPEQISWCFNVA"
     gene            complement(1867..2526)
                     /locus_tag="PLP95_06455"
     CDS             complement(1867..2526)
                     /locus_tag="PLP95_06455"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HPB45479.1"
                     /translation="MTVQPTLDAVFFDFGGVFMDSPFGAMEAAAATLGVDRDLLVDTV
                     FGSYDADTDHPWHRLERGEIELAHAVDAIGEIALGNGLGNFDIFGALAGMVRDGSDRT
                     FMVEVVQRLRGAGVRTSIITNNLKEFGSVWRAHIDVDELFDDVVDSCEVGLRKPNVAI
                     FELAVERLGVSAERSAFIDDFEPNVAAARSVGMHGVWCGYENATTRSAAAELLRLAGL
                     D"
     gene            complement(2523..>3932)
                     /gene="ligA"
                     /locus_tag="PLP95_06460"
     CDS             complement(2523..>3932)
                     /gene="ligA"
                     /locus_tag="PLP95_06460"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020378337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HPB45480.1"
                     /translation="SLRQKDPAITASRGLSFFAYQLGEVVGGPQITRHSEALAWLASL
                     GFPVNPQHRVVSRLEEVEAFCSHWIEHRHELGYEIDGVVVKVDRLDMQQALGVTAKAP
                     RWAIAFKLPPEERTTKLLDIQVSVGRTGKVTPFAVLEPVVVAGSTVAMATLHNEDQVR
                     IKDVRPGDTVIVRKAGDVIPEVVGPVLDLRPEGLEPWVFPATCPCAFHQPLVRVEGEA
                     RHSCVYSACPQTLLARLSHFASRGAMDIDGLGERQLQLFIDLGLVRDVSDIYALDFDA
                     ILAQRGYQETSVNNLRAAIEASKTRPLGNLLFGLNIVHLGAAGGPLLAEGLGSLEAIR
                     AASVEQIASIEGIGPVIATSVHEFFRDPENNRVVDRLLSLGVAPTLPQRSRLEPTLAG
                     MNIVVTGALEGFTRDSVAEAIKARGGKSPGSVSKKTTAVVVGEDPGASKLNKAIELGI
                     AQLDEAQFRHLLDTGELPA"
BASE COUNT          691 a         1336 c         1288 g          617 t
ORIGIN      
        1 acgacacggc gatcgctcat tcgatggatc gaccggaggc gcatcggagt cggcgtcggg
       61 cccggcatcg agcgcaccgg tggcggccga ctcccccgca gcctcgccag ccgccacgtc
      121 caccgactcg tccggcggcg aggttccatc ggcgccgatc gcaatctccg cgaccgctgc
      181 cgtgtccagc gctgccgtgt ccagcgctgt gtcatcgatc gccgcagcat cgaacaggcg
      241 caccgtcgcc ggcgtcgcga tgtcggtgtc cggatccgag tccccagagg ccggctcgcc
      301 cgaaaccggc tcacccaaag ctggggcgcc tgtcgccccc accgctgaca gcacctcggt
      361 cgggtcctcg acctccaaca ccgcggtgag gtcgatcgcc gccacctccg ccgcaacatc
      421 ggcggcgacg gcatcgagtt cggagggcgg gaccgcgctc gcgatcggaa tgtccgccgg
      481 acgggcgaac tgcctcgata gttcgatcaa cgcgagctgt agatcgaccg cggagattcg
      541 ctcctccggg tcgagcttcc cggccgcttc gaggagcgga ccgagcggac ccgcttcggc
      601 cggggcgctc aggtcgtggc caagccgcgc caacgaggtc gcatctgggg tgtcgcgctc
      661 gaacaccgcc tctccgctca cggcctccgc caataccagg gcgagggcat acacgtcggc
      721 agcgggcgac acgagggagc cctgcacttg ttcgggagcg acgtacttcg gggtacccgg
      781 cgacccggcg agatcggtga cactcgactc gctgagcgcc cgggcaacac cgaagtcggc
      841 gatcttgagt tggcgatcga gcccgaacag cagattggcg ggtttgatgt cacggtgcac
      901 gatgccgcgc cggccggcgt gatcgagcgc cgaagcggcg gcgatgccga tcccgatcac
      961 ctgggcgacg gtcagcggcc catggtcgag caccgaacgc agcgaacccc ctccgaggta
     1021 ttccatcgtg atgtacggca atccgttcgc cgcggattcg ttgtagtcgt acagcttgac
     1081 gatgtggcga tgatcgagcg cggccaccgc cttggcctcg gcgcgaaacc ggcgaaggaa
     1141 gcgggtatcg gcggccacga actcgtggag caccttgatg gcgacatcgc gatcgagttt
     1201 caggtcgcgg gcgagataca cctgagcgga ggcgccgctg cccaggaaat acacgatccg
     1261 gtagcggttc tcgaacaaat caccgcgctc aaggagtcga cgcaccatcg tcgccatcgt
     1321 agttgcgcgt cacctgagga ctgtcgacgc gaccggttct cgtcgccgca acccaacggg
     1381 cacaatgggg accatgccga aaaaggtgaa ggccctgctc tacgtcttgt ccgcgtgcgc
     1441 catcgcgctt ggagtgtggg tgtacaacaa cgccgtcacc ggccccgacc agaccagctt
     1501 ggccaccaag ccgtacgtcg atcggctgat cccgacgagc ggttcgcagg caccgcctca
     1561 ggcgacggtg ggcatcgacc tcgaaccggg ctacgacgcg ttcctcgtcg tgaacggcga
     1621 gaagattgcc aacaccattt ccgaggccga ccaggaccca gacgggctcc gcaaggctcc
     1681 gacgctcggc ctcatcgagt acacgcccgg gttgggcaag cgcgtggaac gactcgcgac
     1741 ccctcgcacc tgcgtcgacg cctgggtctg gaaggtcgtc gacggacccg acaaccccga
     1801 acagatcagc tggtgtttca acgtcgcctg acgcggctca ttcgaacgcg gctcagtcga
     1861 ggtcggtcag tcgagaccgg cgaggcgcaa cagctcggcc gcagccgacc gcgtggtggc
     1921 gttttcatag ccgcaccaca ccccgtgcat cccgaccgac ctcgctgcgg cgacgttggg
     1981 ctcgaagtca tcgatgaacg cactccgctc ggcgctcacg ccgaggcgct cgaccgcgag
     2041 ttcgaagatc gccacgttgg gtttgcgcag cccgacctcg cagctgtcga ccacgtcgtc
     2101 gaacaactcg tcgacgtcga tgtgtgctcg ccacaccgag ccgaactcct tgaggttgtt
     2161 ggtgatgatc gaggtgcgca cccccgctcc gcgaagccgc tgcaccacct cgaccatgaa
     2221 ggtgcgatct gaaccgtcgc gcaccatccc ggcgagcgcg ccgaagatgt cgaagttgcc
     2281 taagccgttc ccgagcgcga tttcgccgat cgcgtccacc gcgtgggcga gttcgatctc
     2341 gcctcgctcg aggcgatgcc acggatgatc ggtatcggcg tcgtaggagc cgaacacggt
     2401 gtcgacgagc aggtcgcggt cgacccccaa cgtcgcggcc gctgcctcca tggcaccgaa
     2461 gggagagtcc atgaaaactc caccgaagtc gaaaaacacg gcatcgaggg tgggctgaac
     2521 ggtcacgctg ggagttctcc ggtgtcgagt aggtgacgga actgtgcctc gtcgagttgg
     2581 gcgatgccca gttcgatggc cttgttgagc tttgaggccc ccgggtcctc gccgacgacc
     2641 accgccgtgg tcttcttcga cacgcttccg ggagactttc cgccgcgcgc cttgatcgcc
     2701 tcggcgacgc tgtcgcgggt gaacccctcg agcgctccgg tgaccacgat gttcataccg
     2761 gccagagtcg gctcaaggcg cgagcgttgc ggcaacgtcg gggcgacacc aagcgaaagc
     2821 agacgatcga cgacccgatt gttttccggg tcgcggaaga actcgtgcac cgaggtggcg
     2881 atcaccggcc cgatgccctc gatcgaggcg atctgttcga cgctcgcggc gcgaatggcc
     2941 tcgaggctgc cgaggccctc ggccagcagg ggcccacccg cagcgccgag gtgcacgata
     3001 ttgagcccga acaacaggtt gccgagggga cgggttttcg acgcttcgat cgccgcgcgc
     3061 aggttgttga ccgaagtctc ctggtaaccg cgctgggcga ggatcgcgtc gaagtcgagc
     3121 gcataaatgt cgctgacgtc gcgcaccagc cccaggtcga tgaacagttg cagctgccgc
     3181 tcgccgaggc cgtcgatgtc catcgcaccc cgggacgcga agtgactcag ccgcgccaga
     3241 agcgtctggg ggcaggccga gtacacgcaa ctgtggcggg cctcaccctc gacgcgaact
     3301 agcggctggt ggaatgcgca cggacacgtc gctgggaaca cccacggctc gaggccttct
     3361 gggcgcaggt cgagcaccgg gccgacgacc tcggggatca cgtctccggc cttgcgcacg
     3421 atgacggtgt cgccgggccg cacgtctttg atgcgcacct ggtcctcgtt gtgcagggtg
     3481 gccatcgcca ccgtcgaacc ggcgacgacc acgggttcga gcaccgcaaa cggggtcacc
     3541 ttgcccgttc ggccgaccga gacctggatg tcgagcagct tcgtcgtgcg ttcctcgggc
     3601 ggcaacttga acgcgatggc ccatcggggg gccttggccg tgaccccgag ggcctgttgc
     3661 atgtcgaggc ggtcgacctt caccacgacc ccgtcgatct cgtagccgag ctcatggcga
     3721 tgctcgatcc aatgggaaca gaacgcttcg acctcctcga gccgcgacac cacccggtgc
     3781 tgggggttca ccggaaaccc cagcgacgcc aaccaagcga gcgcctcgga atggcgggtg
     3841 atctgcgggc cgccgacgac ctcgccgagt tgataggcga aaaacgacaa cccacgcgat
     3901 gcggtgatcg ccgggtcctt ctggcgaagc ga
//
LOCUS       DAOOMA010000198         3925 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_27901_length_3925_cov_3.647545, whole genome shotgun sequence.
ACCESSION   DAOOMA010000198 DAOOMA010000000
VERSION     DAOOMA010000198.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 3925)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3925)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3925
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27901_length_3925_cov_3.647545"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..857
                     /locus_tag="PLP95_06465"
     CDS             <1..857
                     /locus_tag="PLP95_06465"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HPB45481.1"
                     /translation="RQRSAAEPQHPQPPAITGLEEIAVFPLAIPISFTDTHGAPRSGG
                     RSHQGQDLMASKGTVAIAVVDGTITQIKHSNDGLAGNQLRLTGDNGWTYYYIHLNNDT
                     PGTDDGANRYDQAFVDGIRVGQRVRAGEPVGFVGDSGNAESTAPHLHFEAHHPTLGVV
                     NSYSYLKAAPVWMRDTSSTRTFGSLDSVSSTAANTISVAGWALAPGTDDAAGYSVYLN
                     GNPAYNAKASTPRPDVAAVYPTYGPNHGFAATIPGVVSGQHEVCVVLHNPGPGGGSRT
                     GCATVTVA"
     gene            complement(876..1313)
                     /locus_tag="PLP95_06470"
     CDS             complement(876..1313)
                     /locus_tag="PLP95_06470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="group III truncated hemoglobin"
                     /protein_id="HPB45482.1"
                     /translation="MTSLAPTTVEPPTPNGDLDSIDEVAELVRRFYADVAQDDLLGPL
                     FNDVAQVDWSLHLPKLTAFWSRALLQQPGYEGNPYRMHQLIHDQSPFTVAHFHRWLDL
                     FTETIALGWAGPRADHAKALALRVAQVHCSHIVGEELDLSAYA"
     gene            complement(1376..1534)
                     /locus_tag="PLP95_06475"
     CDS             complement(1376..1534)
                     /locus_tag="PLP95_06475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB45483.1"
                     /translation="MSDQTPPDRSDDRTRRPNDPVPRPQHPRGAAIAVVVVMILLIVL
                     LAVASTFG"
     gene            1559..1990
                     /locus_tag="PLP95_06480"
     CDS             1559..1990
                     /locus_tag="PLP95_06480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015439958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HPB45484.1"
                     /translation="MRLEITRRSDLATRALIELAHRGERTKAGDLAAAVGTTPGFLSQ
                     AMTPLVAKGWARSEPGPSGGYQLVADLSSISVLDVIEAIEGPSDGGRCVLQDRPCGES
                     GTCALHFPWTRARTQLLEELSATSLDSLDTLANPVPGRTAL"
     gene            2063..2761
                     /gene="ric"
                     /locus_tag="PLP95_06485"
     CDS             2063..2761
                     /gene="ric"
                     /locus_tag="PLP95_06485"
                     /inference="COORDINATES: protein motif:HMM:TIGR03652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster repair di-iron protein"
                     /protein_id="HPB45485.1"
                     /translation="MEITPDATLATIVTGVRGAPRILEGFGLDYCCHGNRSLADACAE
                     ASLNLGEVIDQLSAEFSRDPAAADLPEDTVELVDHILSTHHRYLDAELPRLEALAIKV
                     EGVHGDRHPELHRVRELCTALRDDLTPHMLKEENVLFPLIRSLVTGEPSTGPTMPVDR
                     PVAMMMMEHEATGEILEELRNVTSGYEAPGDACGSYQALYAGLADVERDTHMHIHKEN
                     NVLFPKALSLVAVG"
     gene            3083..>3925
                     /locus_tag="PLP95_06490"
     CDS             3083..>3925
                     /locus_tag="PLP95_06490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB45486.1"
                     /translation="MLSTFGGQTTFFASAESGSRTVGLVLAYEAGDVSTISFDLPVSD
                     QYAPSAIEVDGEGWYVLAPGCFEESKPCEDGTVNILSVSNGRETADLLAAFDPSGEAE
                     LVGAVGEQLVVSVLGDHGSRIVLVNSMTGDVQELEWRPPIYSSSEIRAKANEAESSDK
                     FDIPRTSYCVAPTKVWVLTAPAVIEGNQQGDDSLTSIELLDDRRATSVSIGESVQRSA
                     ASLACDDSGLSTVGRSGGTVTQYRIDLSGLVSGKALYRKSVLDDELVVYSAAQGGVFS
                     DLVAP"
BASE COUNT          759 a         1183 c         1247 g          736 t
ORIGIN      
        1 cgcggcaacg gtccgccgcc gagccgcagc acccgcagcc gccggcgatc accgggctcg
       61 aggagatcgc cgtgttcccg ctggcgatcc ccatctcgtt caccgatacc cacggcgcac
      121 ctcgctcggg tgggcgcagc catcagggac aagatctgat ggcctcgaag ggtacggtcg
      181 ccattgcggt cgtcgacggc acgatcaccc agatcaagca ttccaacgac gggctggcgg
      241 gcaatcagct ccgactgacc ggcgacaacg ggtggaccta ctactacatc catctcaaca
      301 acgacacgcc cggcaccgac gacggcgcaa atcgctacga ccaggccttc gtcgacggca
      361 ttcgggtcgg ccaacgggta cgggcgggtg aacccgtcgg gttcgtcggt gactctggaa
      421 acgccgagtc gaccgcaccg cacctgcatt tcgaggcgca tcatcccacg ctcggggtgg
      481 tcaactccta cagctacctg aaggccgctc cggtgtggat gcgcgacacg tcctcgaccc
      541 gcacattcgg atcgctcgat tcggtgagtt ccaccgctgc aaacaccatt tcggtggcgg
      601 gctgggcgct ggctccaggg accgatgatg cggccggcta ctccgtgtat ctcaacggca
      661 atcctgccta caacgcgaaa gcttcgacgc ctcgccccga cgttgccgcg gtctacccga
      721 cgtacggccc gaaccacggc tttgcggcga ccatccccgg cgtcgtgagc ggacaacacg
      781 aggtgtgcgt ggtgttgcac aaccccggac ccggtggcgg ctcccgaacg ggttgcgcga
      841 ccgtgacggt ggcctgaccg actcgaggtg attcgctacg cgtacgcgct gaggtcgagt
      901 tcctcgccga cgatgtgaga acagtgaact tgggcaacac gaagcgcgag ggcctttgcg
      961 tgatcggccc gaggcccagc ccagcccagt gcgatggtct cggtgaacag gtcgagccag
     1021 cgatgaaagt gcgccacggt gaacggcgac tgatcgtgga tgagttggtg catccggtag
     1081 gggttgccct catagccggg ctgttgcagc aacgcccgtg accagaacgc ggtcagtttc
     1141 ggcagatgca gtgaccagtc gacctgggcg acgtcgttga acagtgggcc cagcagatcg
     1201 tcctgggcga cgtccgcgta gaagcggcgg acgagttcgg cgacttcgtc gatgctgtcg
     1261 agatccccgt tcggcgtggg cggctcgacc gtggtcgggg cgagtgatgt catgtccgcg
     1321 gctccttgaa gtcagggtgg gcgatgggcc gacgctgttg cggcgttgtt tcgggctatc
     1381 cgaaggtgct ggcgacggcg aggagaacga tgagcagaat catcacgacg accacggcga
     1441 tcgcggcacc gcgcgggtgc tgcgggcgcg gaaccgggtc gtttggccga cgcgtgcggt
     1501 cgtcggatcg atcgggaggg gtctggtcac tcatcattct attgtatagt atacgactat
     1561 gcgattggag atcacccgac gatccgatct ggccaccagg gcactcatcg agttggccca
     1621 tcgcggcgag cgaaccaaag cgggcgacct tgcggcggcg gtcggcacga cgccggggtt
     1681 cctttcccaa gcgatgaccc cgctcgtggc caaggggtgg gctcgatccg agccggggcc
     1741 ttccggcggc tatcaactcg tcgccgacct gtcctcgatc agcgtgctcg atgtgatcga
     1801 ggccatcgag ggaccctctg atggcggccg gtgcgtgttg caggatcgtc cgtgtggcga
     1861 aagcggcacc tgcgcgttgc acttcccgtg gacccgcgct cgaacacaac ttctcgaaga
     1921 gctttcggcg acgtcgctgg acagcctgga caccctcgcc aatccggtcc ccggtcgcac
     1981 ggcgctctga ccacggccaa agccctcagg agccaacgga ttcccaacga cagaacatca
     2041 ccacctaccg aggagcaaca tcatggagat cacaccggac gccacgctcg cgaccatcgt
     2101 cactggggtt cgcggtgcac cccgaatcct tgaggggttc ggtctcgact actgctgtca
     2161 cggaaaccgc agcctggccg atgcctgtgc cgaggcctcg ttgaacctcg gcgaggtcat
     2221 cgaccaactt tctgccgaat tctcccgtga tcctgccgca gcagaccttc ccgaggacac
     2281 cgtcgaactg gtcgaccaca tcttgtcgac acatcaccgc tacctcgatg ccgagctgcc
     2341 ccgcttggag gcgttggcga tcaaggtcga aggagttcac ggtgaccgtc atcccgaact
     2401 gcaccgggtc cgcgagttgt gtacagcgct gcgagacgac ctcaccccgc acatgctcaa
     2461 agaggagaac gtgttgtttc ctctgatccg ttcgctcgtc accggcgagc cgagcaccgg
     2521 gccgacgatg ccggtcgacc gtccggtggc gatgatgatg atggaacacg aggcaaccgg
     2581 cgagatcctc gaagagctcc gcaacgtgac gagtggctac gaggcgcccg gcgacgcctg
     2641 cggcagctat caggcgcttt atgccgggtt ggccgacgtc gaacgcgaca cacatatgca
     2701 catccacaag gagaacaacg tgttgttccc caaggcgttg tcgttggtag cggtcggcta
     2761 aaccaacgcg aacccagtat ccaagggaac tcatttggca agccttgaga tgggtgccac
     2821 agcgtgtcta taaccccgac tgtcgagacc ggttcgaaac ccacagaagc acagggcggg
     2881 tgacgctacg acaccctcag ggctgatgga ttgcgttagg gtcctggcga aatcgaatag
     2941 aggggacagt cgcttgagat tcataggtct catcagttgc gtcgcagcga cggtgttgct
     3001 ttccgggtgc ggctcggtcg agcctgaacg ggcacaagga gcaccatctg ccgaactcgc
     3061 gggtgttcca aaggtcgatc gtgtgctttc gacatttggt gggcaaacga cctttttcgc
     3121 gagcgccgag tccggtagtc gtacggttgg actagttttg gcctacgagg ctggcgatgt
     3181 ctcaacgatt tcgttcgatt tgccagtgag cgatcaatac gcacccagcg caatcgaagt
     3241 ggacggcgaa ggctggtatg tcttggcgcc gggttgcttt gaagaatcta agccgtgtga
     3301 agacggcaca gtcaacattc tttccgtgtc gaatggacgt gagaccgccg atctattagc
     3361 agctttcgac ccttcgggtg aggcagaact agttggcgca gttggcgagc aactagtcgt
     3421 cagcgtactc ggtgaccatg gcagcagaat cgtcttggtc aattctatga cgggcgacgt
     3481 ccaagagctt gagtggagac cgcctattta ctcctcgagc gagattcgag cgaaggccaa
     3541 cgaagccgag agttccgaca agttcgatat tccacgaaca tcgtattgtg tagccccaac
     3601 taaggtctgg gtactcacgg cgccagcggt gattgagggt aatcaacaag gcgacgactc
     3661 gctcacgtcg attgagcttt tggatgatcg gcgggccacg tccgtttcga ttggtgagag
     3721 cgtgcaacgg tccgctgcat ccttagcatg tgacgacagc ggactaagta cagttggtcg
     3781 ctcggggggc acggttactc aatacagaat tgacctgagc ggtctcgtct cggggaaagc
     3841 gctgtatagg aaatcagttt tggatgatga gctcgtcgtc tacagtgccg cccaaggcgg
     3901 ggtattctcg gacttggtcg ccccg
//
LOCUS       DAOOMA010000199         3918 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_27970_length_3918_cov_4.139011, whole genome shotgun sequence.
ACCESSION   DAOOMA010000199 DAOOMA010000000
VERSION     DAOOMA010000199.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 3918)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3918)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3918
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27970_length_3918_cov_4.139011"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            240..911
                     /locus_tag="PLP95_06495"
     CDS             240..911
                     /locus_tag="PLP95_06495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB45487.1"
                     /translation="MRHRRAQASHALVVGLLGTLSMLAAHCGGYQLVYLLESPAAPDT
                     MAMSVHPGHGHHAAMSPPVVDAHSGHAHVVPVASGAFLALLVVLVCLGALATRRIRVP
                     PPSASALFAVQVGLLVSMEGVERHIAGVDPVAMVADRRVLLAIALQLPAALLVARLGR
                     KVVEVAAVVAAVVAAAFGRVPRRVRVAAAIPERVAARPVRLQSAQRGWPPSLRGPPRN
                     RPVPI"
     gene            959..1645
                     /locus_tag="PLP95_06500"
     CDS             959..1645
                     /locus_tag="PLP95_06500"
                     /inference="COORDINATES: protein motif:HMM:NF019599.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcnI family protein"
                     /protein_id="HPB45488.1"
                     /translation="MTTNIFKPAAFAGASIAALFALTPVASAHVTPVPSTAPAGGYAT
                     IELQIPHGCDGAATERVEVQLNDEISSVKAESIAGWTVSYERAPLDEPIDLHGEQVDE
                     YVSVVSWTAEGDPLPDDEYQRFGISMKVPEHAGDDLLLPTVQYCVGGGTSSWLEADPE
                     AEHPAPMVKLVAADGTDGHDDAPSEDSADSADNAEAESSSDAVARVLGVVAIALAAVG
                     AGLALRKRPA"
     gene            1742..3301
                     /locus_tag="PLP95_06505"
     CDS             1742..3301
                     /locus_tag="PLP95_06505"
                     /inference="COORDINATES: protein motif:HMM:NF017262.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper resistance protein CopC"
                     /protein_id="HPB45489.1"
                     /translation="MTFHRTISVSKATAVVVAVLLSFVAVTPAAGAHAALVTTDPPNG
                     AHLDTPIDEVVFTFNESVQARSFVLVDADGNRTELAPSGGDVMRAPVDTSEGGWFALS
                     WSVVSADGHSIGGAITFRVGAGDDATPDDLASLTDGGTGPTGPWEMLRVLGRSLAILG
                     AIVAIGTAFLRRWALGPDLDGALAPVRTLAAAAAILSVLGSATAFGAAVGGDSVIWPG
                     SAADLVIGVGALSTLAWSARRREDSTMAGIVAIVGLFSLALIGLSGHVAADGRPLARM
                     ALAAHLVAATVWLGATPALALALRSGAGWRVLRRFSTTATATIGVVVVAGGVVTWSLS
                     DGWASLTSTAWGRLLIIKALLVGLALALGAGNRRRVRNERDLRGTGRLVAFEALVLVG
                     VLIFTAGLARSSPRYDDGPDRSARSVSVAMGDLTARVEIGEEIDASREIRVTFVDAGN
                     VVVDVAEPTLELSAEDLGVAAIPQDLEAVDTGRYRTVTADLRLRGAWTVHLSAKPDRF
                     RIEDGEVTLDL"
     gene            complement(3285..>3918)
                     /locus_tag="PLP95_06510"
     CDS             complement(3285..>3918)
                     /locus_tag="PLP95_06510"
                     /inference="COORDINATES: protein motif:HMM:NF024118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HPB45490.1"
                     /translation="DVVLTSHIHMDHLHMASLRLLLHGRERPLSVVVPHGARRLLPSD
                     ERISVTETRVGHTHRFASVTVETVKAVHSHSRGPHRRVRAEAVGFVVRGADTSVYFAG
                     DTDLFDEMAHLNADVSLVPIGGWGRTVGPGHLDAHSAVDAVRLLGDATVVPIHWGTYS
                     PLALRAGPPQWLTRPVETFASNLTEAGLSGRVHLLEPGGTMNPEVATDPT"
BASE COUNT          551 a         1323 c         1374 g          670 t
ORIGIN      
        1 cacgatcatc ttcaccaggt acatgaccag gaccacgtcg ggggagccga agccgtactc
       61 cgcgaagtgc cgggtcatcg gcgcgatgcg ctcctggcga ctctggtgat cccacacgtc
      121 gtggtcgacg acgggaaggt tcggttggag aaaccccatc gttcgcacgc cccctgcagt
      181 gaccagcggt cgaagccgac cgggacatca gggtagtgct tggcagtagc atccggccga
      241 tgcggcatcg gcgtgcgcag gcgtcccacg cgctggtggt ggggttgttg gggacgctga
      301 gcatgctcgc ggcccactgc ggcggctatc agctggtgta cctcctcgag tccccggcgg
      361 ctcccgacac gatggccatg tcggttcacc cgggccacgg acaccacgcg gcgatgagtc
      421 cacccgtggt cgacgctcac agcggccatg cacacgtcgt gccggtggcc tcgggggcct
      481 tcttggcgct cctcgtcgtg ttggtctgcc tcggggcgct ggcaactcga cgaatccggg
      541 ttcctccgcc gtcggcctcg gcgttgtttg cggttcaggt cgggttgttg gtctcgatgg
      601 agggcgtgga acgacacatc gccggggtgg acccggtcgc catggtcgcc gatcgtcggg
      661 tcctcctcgc gatcgccctg cagttgccgg cagcgttgct ggtggcccgc ctgggccgaa
      721 aggtcgtcga ggtcgcggcg gtggtcgcgg cagttgtcgc tgcggcgttc ggccgggttc
      781 cgcgtcgggt ccgtgtcgcg gctgcgatac ccgaacgggt tgcagcacgc ccggtgcgtc
      841 ttcaatcggc acaacgcggc tggccaccct cgcttcgcgg gcctcctcga aatcgacctg
      901 tcccgatctg acatccgatt tcactcgcag ttcccacgag ccaggaggct cgcaacacat
      961 gaccacaaat atcttcaaac ccgccgcgtt cgctggcgcg tccatcgccg cgctcttcgc
     1021 actgacgccg gtggcatccg cacacgtcac ccccgttccg tcgaccgcac ccgctggcgg
     1081 gtacgcaacg atcgagttgc agattcccca cggctgcgac ggcgctgcga ccgaacgggt
     1141 cgaggtgcaa ctcaacgacg agatcagctc ggtgaaggcc gagtccattg ccggatggac
     1201 ggtctcctac gagcgggctc ctctcgacga gccgatcgac ctgcacggtg agcaggtcga
     1261 cgagtacgtc tcggtcgtgt cctggacggc cgagggcgac ccgctgcccg acgacgagta
     1321 ccaacgcttc ggtatctcca tgaaggtccc cgaacacgct ggcgacgacc tgttgttgcc
     1381 gacggtgcag tactgcgtcg gcggcggaac atcgtcgtgg ctcgaggccg accccgaggc
     1441 cgagcacccg gccccgatgg tgaaactcgt cgcagccgac ggtaccgacg gccacgacga
     1501 cgcgccgtcg gaggacagcg ccgacagcgc cgacaacgcc gaggccgagt caagtagcga
     1561 cgccgtcgcc cgcgtcctcg gcgtggtggc gatcgcactc gcagcggtcg gggcgggcct
     1621 cgcccttcgc aaacgcccgg cctgatcgcc gagttggggg gcacgtcggc caggtcgccg
     1681 gcgtgacccc ttaccccacc cgatccgacc ttgcgtctcg atcagaaacg tgccccacgt
     1741 catgaccttt caccggacca tctcagtttc gaaggcgaca gcggtcgtgg tcgccgtcct
     1801 gctgtcgttc gtcgccgtca cccccgcggc cggagcccac gctgcgctcg tgacgaccga
     1861 tccgcccaac ggtgcgcacc tcgatacgcc aatcgacgag gtggtgttca cgttcaacga
     1921 gtccgttcag gcccgctcgt tcgtcctggt cgacgccgat gggaaccgca ccgaacttgc
     1981 gccgtcgggg ggcgacgtaa tgcgggcgcc cgtcgacacc agcgaagggg gatggttcgc
     2041 gctgtcgtgg agtgtcgtgt cggccgacgg ccattcgatc ggtggtgcga tcacgttccg
     2101 agtcggggcc ggtgacgacg cgacgcccga cgatctcgcc tcactgaccg atggcggcac
     2161 gggcccgacc ggaccgtggg agatgctgcg cgtgctcggc cgcagcctcg ccattctcgg
     2221 agcgatcgtc gccatcggca ccgcgtttct tcggcgctgg gcattgggcc cggatctcga
     2281 cggcgctctc gcaccggtgc gcaccttggc tgcggccgcc gcgatcctgt cggttctcgg
     2341 ctcggcgacg gccttcggag ccgcggtcgg tggcgactcg gtgatctggc cgggaagcgc
     2401 cgccgacctg gtgatcggtg tcggcgcgct gtccaccctg gcctggtcgg cgcggcgccg
     2461 cgaggacagc acgatggctg gcatcgtcgc gatcgtcggc ctgttctcgc ttgcgctgat
     2521 cggcctgagc ggccacgtcg ccgccgatgg gcgcccgctg gcgcggatgg cgctcgccgc
     2581 ccacctcgtc gcggcgacgg tctggttggg tgcgactcct gcgctcgccc tggcgttgcg
     2641 ctcgggggcc gggtggcgtg ttctgcgccg gttctcgacg acggcgaccg ccacgatcgg
     2701 ggtcgttgtt gtggccggtg gcgtcgtcac ctggtcgctg tcggacggct gggcctcgtt
     2761 gacctccacg gcgtggggac gtctgttgat catcaaggcc ctcctggtcg gcctggcgct
     2821 ggcgctgggt gcgggcaatc gtcgtcgggt ccgcaacgaa cgggaccttc gcggcacagg
     2881 ccgtctcgtg gcgttcgagg cgctcgtgtt ggtgggggtg ttgatcttca ccgccggttt
     2941 ggcacgcagt tcgccgcggt acgacgacgg tccagatcgt tccgcccgat cggtttcggt
     3001 ggcgatggga gacctcaccg cacgggtcga gatcggcgag gagatcgatg ccagtcgtga
     3061 aatccgggtc acgttcgtcg acgccggaaa cgtggtggtg gacgtggccg aaccgaccct
     3121 cgagctgtcc gccgaagacc tcggcgtcgc tgcgatcccc caggaccttg aagcggtcga
     3181 taccggcaga tatcgaaccg tcaccgccga ccttcgcctc cgtggcgcct ggacggttca
     3241 cctgagtgcg aagcccgacc ggtttcgaat cgaggacggc gaagtcacgt tggatctgta
     3301 gcgacctcgg ggttcatcgt cccgccgggt tccaacaggt gaacccgccc gctgaggccg
     3361 gcctcggtga ggttcgaggc gaaggtctcc acggggcggg tgagccactg cggcggaccc
     3421 gcccgaagcg caagcgggct gtaggtgccc cagtggatcg gcacgacggt ggcatcgccg
     3481 aggaggcgca cggcatccac cgcgctgtgg gcgtcgaggt gcccgggccc gaccgtgcgt
     3541 ccccagccgc cgatgggcac gagcgaaaca tcggcgttca ggtgcgccat ctcgtcgaac
     3601 aggtcggtgt ctccggcgaa gtagaccgac gtgtcggccc cgcggacgac aaagccgacc
     3661 gcctccgctc gcacgcggcg gtgtggacct cgcgagtggg aatgcaccgc cttgaccgtc
     3721 tcgacggtca ccgaggcgaa ccggtgggtg tggcccaccc gggtttcggt gacggagatc
     3781 cgttcgtcgc tcggcaacag tcgtcgcgcg ccgtggggca ccacgaccga cagtggacgc
     3841 tcgcggccgt gcagcagcag gcgcagcgaa gccatgtgca ggtggtccat gtggatgtgg
     3901 gaggtgagca cgacgtcg
//
LOCUS       DAOOMA010000200         3916 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_27995_length_3916_cov_3.505828, whole genome shotgun sequence.
ACCESSION   DAOOMA010000200 DAOOMA010000000
VERSION     DAOOMA010000200.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 3916)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3916)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3916
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27995_length_3916_cov_3.505828"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(64..903)
                     /locus_tag="PLP95_06515"
     CDS             complement(64..903)
                     /locus_tag="PLP95_06515"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021623.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HPB45491.1"
                     /translation="MGAPSGPSRAEPPRHRRVSFRFGDSVDANWHRALPEFAAAANAV
                     SLMMPYVEPFVIRSMRDQLPHLDRLADPLLAAQARAYIAQEAQHQAQHRRFNESIRAQ
                     VRGVGRLERLLDWTYRTLWERSSDRFRLAFATASESAAYGVARWVERRHAELFTGADP
                     KVAALYLWHLAEEVEHKSVAFDVYDARHRSRRPLAAAMVLSVILLAAFTVAGTVLGLI
                     HQRRLFNPFAVMHLVVWGFGFAFSELPNMFASVLPGHHPSEFVDPTFFELYLLDIESR
                     AAA"
     gene            1012..1176
                     /locus_tag="PLP95_06520"
     CDS             1012..1176
                     /locus_tag="PLP95_06520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB45492.1"
                     /translation="MGQLARQPKNEDSKQGGVAAELFMEQCDQCGAPVTIVGVKPETI
                     RCPRCTDASS"
     gene            1209..1940
                     /locus_tag="PLP95_06525"
     CDS             1209..1940
                     /locus_tag="PLP95_06525"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HPB45493.1"
                     /translation="MVWSYDFNEGRLRQRARKAIYAAAGPVVRWTWDTYKSMGAIGPN
                     SKAGRAFGSLGEGSIVCFPYDTIMNPQAIHIGSGTMISGHGVLSAGWGPNHPGLADRV
                     VVIGDRCLIGRGSSIVGHRLIEIGDDVWTGHNVHITDMNHGYEDLDVPISQQSMGELP
                     VHIGSGSWLGHNTVVLPGVTIGRHVVVGAGSIVTGDLPDYSVAVGSPARIVRVHDPDL
                     GWKRPGPEAVTKDFENAAPEGDQVV"
     gene            1950..3461
                     /locus_tag="PLP95_06530"
     CDS             1950..3461
                     /locus_tag="PLP95_06530"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF012946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HPB45494.1"
                     /translation="MAPTVEASPRIAIFGAGAAGICTAIKLIEAGLDDITMFEKSDGV
                     GGTWRANTYPGAACDVPSHLYSYSFASKGDWTRKFAQQPEILAYFEEITDRYGLRRLI
                     RFNTEVRSATFDENTGTWRVVTDAGAEDFDVVVSGLGQLNRAYVPEIDGLSQFAGDMF
                     HSAHWDHSVDLSGRRVAVVGNGASALQFIPHVAEQASELTVFQRSANWVIDKRDGEYP
                     GWRQRMFARFPATQRLHRWSIYWRLEANFALLRRSSRVARLLERVITRALEPVTAVLP
                     SEAVIPDYPIGCKRILLSSDYYAALMRDNVHVELDHIDHIEPDAVVTVDGRRHEVDTI
                     IWGTGFRSTEFLAPMTVIGRDGRDLNEAWAKGAVAYIGVAVAGFPNLFMLYGPNTNLG
                     HNSIIFMIEQQSRYIVEMVKELRDQHLAWIDVRRDAMDRYNVDVQRRAGDTVWVASCD
                     SWYKNEHGIVTNNWPAFTVTYWKRMRGIRRGDHVRRHRAVAATTSPELRHEIV"
     gene            3448..>3916
                     /locus_tag="PLP95_06535"
     CDS             3448..>3916
                     /locus_tag="PLP95_06535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012228734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HPB45495.1"
                     /translation="MKLSEILSLSSHGPETFVGTGPRYPWGGLYGGQIVAQSLLSAAS
                     SVDDGLAVHSLRAYFIRRGDQDEPVRYEVERLRNGRSFATRRVVARQATGAILNLEAS
                     FQADEASVDLDHVVLPADLPPPSELEVTGWSDEFFRFFQAEDGIRDDTAGIGRA"
BASE COUNT          698 a         1238 c         1325 g          655 t
ORIGIN      
        1 ccggcgatac gcccaagcga gcgctgcgag ctcggcaccc gaaggggtcg ggcccgttca
       61 aggtcacgcc gcggcgcggg actcgatgtc gagcaggtac agctcgaaga acgtcgggtc
      121 gacgaattcg ctcggatgat ggccgggtag caccgacgcg aacatgttcg gcagttcgct
      181 gaaggcgaac ccgaaccccc acacgacgag atgcatcacc gcgaaggggt tgaacaagcg
      241 ccgctgatga atcaacccga ggaccgtgcc ggccacggtg aaggccgcca gcaggatcac
      301 cgacagcacc atcgctgcag cgagcggtcg acgggagcgg tgccgggcgt catacacgtc
      361 gaaggccacg ctcttgtgtt cgacctcctc ggcgaggtgc cacaggtaca gcgcggcgac
      421 cttgggatcg gccccggtga acaactcggc gtgacggcgc tcaacccacc tggccacccc
      481 gtaggccgcc gactccgagg cggttgcgaa ggccaaacga aaacgatcgc tgctgcgttc
      541 ccacaacgtc cggtaggtcc agtccaacag tcgttcgagc cggccgaccc cgcgcacctg
      601 ggcacggatc gactcgttga aacggcggtg ttgggcctgg tgctgggcct cctgggcgat
      661 gtaggcccgc gcctgggccg ccagcagcgg gtcggcgagc cgatcgaggt gcggcagttg
      721 atcgcgcatc gaccggatga cgaacggttc gacgtagggc atcatcagcg acacggcgtt
      781 ggcggccgcc gcaaactcgg gcagggcgcg gtgccagttc gcgtcgacgg agtcgccgaa
      841 acgaaacgac acccgtcggt ggcggggagg ttcggctcga ctcggacctg agggcgctcc
      901 catggttgat ttgtcggccg acgcccccga aacctgagcc gtcgcagcgc catctggttc
      961 gggtgggcgc cacgcgcgtt caagtcggtg ctctcggcgt aggctgcgcc catgggtcaa
     1021 ctggcacgtc agccgaagaa cgaagattcc aagcagggcg gcgtggccgc cgagttgttc
     1081 atggaacaat gcgaccaatg cggcgcaccc gtcacgatcg tcggggtcaa acccgagacg
     1141 atccggtgcc cgcgctgcac cgacgcctca agctgacccc ggaaacgaac tgttagcgtc
     1201 gcccggcgat ggtctggagt tacgacttca acgagggcag gttgcgtcag cgggcgcgaa
     1261 aggcgatcta tgccgccgct gggccggtgg tgcgctggac ctgggacacc tacaaatcca
     1321 tgggcgcgat cggtccgaac tcgaaggccg gccgtgcgtt cgggtcgctc ggcgagggca
     1381 gcatcgtgtg tttcccgtac gacacgatca tgaaccccca ggcgatccac atcggttcgg
     1441 gcacgatgat cagcggccat ggcgtgctgt cggccggctg ggggccgaac cacccgggcc
     1501 tggcggatcg ggtcgtggtg atcggcgacc gttgcctcat cgggcgcggg tcgtcgatcg
     1561 tcggacatcg gctcatcgag atcggcgatg acgtctggac cgggcacaac gtgcacatca
     1621 ccgacatgaa ccacggctat gaagatctcg acgtccccat cagccagcag agcatggggg
     1681 agttgccggt ccacatcggg tcgggttcgt ggctcggcca caacacggtc gtgctgccgg
     1741 gggtcaccat cggacgccat gtggtggtcg gcgccggaag catcgtgacc ggtgatctgc
     1801 cggactacag cgtcgcggtt ggcagtccgg cgcggatcgt gcgcgttcac gatcccgatc
     1861 tgggttggaa gcggccgggg cccgaggccg tcacgaagga ttttgagaac gcggctcccg
     1921 aaggagacca ggtcgtctaa aacgtggcca tggcaccaac cgtcgaggcg tcacccagga
     1981 tcgcgatctt cggagccggt gctgcgggca tctgcaccgc catcaaactc atcgaagccg
     2041 gcctcgacga catcacgatg ttcgagaagt ccgacggggt agggggaacg tggcgggcga
     2101 acacctatcc gggtgcggcc tgcgatgttc cttcgcatct gtactcgtac tcgttcgcgt
     2161 cgaagggcga ctggacccgg aaattcgcac agcaaccgga gattctcgcc tacttcgagg
     2221 agatcaccga ccgctacggg ctccggaggc tgatccggtt caacaccgag gtccgctcgg
     2281 cgaccttcga cgagaacacc gggacgtggc gggtggtcac cgacgctggc gccgaggact
     2341 tcgacgtcgt cgtgtcgggg ctcggacagc tcaatcgcgc ctatgttccc gaaatcgacg
     2401 ggctgtcgca gttcgccggc gacatgttcc actcggcgca ttgggaccac tcggtcgatc
     2461 tgtcgggacg tcgagtcgca gtcgtcggta acggggcgag cgcgctgcag ttcatcccgc
     2521 acgtggccga acaggcctcg gagctgacgg tgttccaacg cagcgcgaac tgggtgatcg
     2581 acaagcgcga cggcgagtac cccgggtggc gtcagcgaat gttcgcccgg tttccggcga
     2641 cccagcggct gcaccgatgg tcgatctact ggcggctcga ggcgaacttc gcgttgcttc
     2701 gccgctcctc tcgcgtggcg cggctgctcg aacgcgtcat cacgcgagcc ttggagcccg
     2761 tcaccgcggt gctgcccagc gaggcggtga ttcccgacta tccgatcgga tgcaagcgga
     2821 tcctgctgtc gagcgactac tacgcggcgt tgatgcgcga caacgtgcac gtcgaactcg
     2881 accacatcga ccacatcgaa cccgacgcgg tggtcaccgt cgatggtcgg cgacacgagg
     2941 tcgacacgat catctggggg accgggttcc gctcgacgga gttcctcgcc ccgatgacgg
     3001 tgatcggccg tgacggccgc gatctgaacg aggcctgggc caagggcgct gttgcctaca
     3061 tcggtgtggc ggttgcgggg ttcccgaacc tgttcatgct ctacggtccc aacaccaacc
     3121 tcggccacaa ctcgatcatc ttcatgatcg aacagcagag ccgctacatc gtcgagatgg
     3181 tcaaggagtt gcgcgatcaa cacctcgcct ggatcgacgt gcgccgagac gccatggatc
     3241 gctacaacgt cgacgtgcaa cggcgggcgg gcgacaccgt ttgggtggcc tcctgtgata
     3301 gttggtacaa gaacgaacac ggcatcgtga ccaacaactg gccggcgttc acggtgacct
     3361 actggaaacg catgcgcggg attcgtcgag gtgaccacgt gcgtcgccat cgcgcggtgg
     3421 cggcaacaac ctccccggag cttcgtcatg aaattgtctg agattctgtc gctgtcgtcc
     3481 catggtcccg agaccttcgt gggaacgggg ccgcgttacc cctggggcgg cctctacggg
     3541 ggtcagatcg tcgcccagtc gctgctgtcg gcggcatcga gcgtggatga cggcctcgcg
     3601 gtccattcgc tgcgcgcgta cttcatccgt cgaggcgacc aggatgagcc cgttcgctac
     3661 gaggtggagc ggcttcgcaa cggacgatcg ttcgccaccc gccgggtggt ggcgcggcag
     3721 gccactggcg ccatcctcaa cctcgaggcg tcgttccagg ccgacgaggc ctcggtcgac
     3781 ctcgaccatg tggtgttgcc ggcggacctt ccgcctccgt cggagcttga ggtcacggga
     3841 tggagcgacg agttctttcg tttttttcaa gcagaagacg gcatacgaga tgacaccgca
     3901 ggaattggac gggcca
//
LOCUS       DAOOMA010000201         3899 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_28137_length_3899_cov_4.994537, whole genome shotgun sequence.
ACCESSION   DAOOMA010000201 DAOOMA010000000
VERSION     DAOOMA010000201.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 3899)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3899)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3899
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28137_length_3899_cov_4.994537"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..393)
                     /locus_tag="PLP95_06540"
     CDS             complement(<1..393)
                     /locus_tag="PLP95_06540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB45496.1"
                     /translation="MKSLVIAEAFPWPTDDGYRLRLANLLGAFLELGEVDFLCLDGRR
                     RTRDHAPEGVTVIDAPGGPDRAISSWLRPWLTGSDPRRLLRSDFSEGRRRLETLDQSN
                     YDVCFVSHVDCTHKLETYGKQISFDLSDP"
     gene            451..1203
                     /locus_tag="PLP95_06545"
     CDS             451..1203
                     /locus_tag="PLP95_06545"
                     /EC_number="4.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020378404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
                     /protein_id="HPB45497.1"
                     /translation="MSFLSISRHGRVAVLTLDDPKRRNALNLDLNAELIEAISDIEAD
                     HSLGAIVITGAAPAFCAGADLSQLGASQHEGLKRIYAGFLAVANCSLPTIAAVNGAAV
                     GAGMNLALACDVRVAGASARFDTRFLDLGLHPGGGHTWMMRRIVGPAAAAAMVLFGQI
                     LSGEEAKRIGLAWDCVADEELVEYCISMADRAASAPRELVIKVKASLGAMASIDDHSE
                     AVAAELEPQVWSLNEPAFAERLAALQAKITSR"
     gene            complement(1242..1886)
                     /locus_tag="PLP95_06550"
     CDS             complement(1242..1886)
                     /locus_tag="PLP95_06550"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HPB45498.1"
                     /translation="MWTRRSMQKSLGTAPVAQGVTLIGRRVVLRPLSSADFDQWSEVR
                     LSSEEWLTKWEPARVPGAPDIVEDRQAFAARCGARGREIQLGSGYSFGIFIDGSFGGE
                     INISSVHRGPFQSAYVGYWIDVRRAGHGYMPEALVLVMQFAFEQMGLHRLQVSIVPRN
                     AASRRVADKLNLRNEGIAERYLEINGVWEDHVRYAITSEEWQQRGPELLSTWTY"
     gene            complement(1898..2821)
                     /locus_tag="PLP95_06555"
     CDS             complement(1898..2821)
                     /locus_tag="PLP95_06555"
                     /inference="COORDINATES: protein motif:HMM:NF014587.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ppx/GppA phosphatase family protein"
                     /protein_id="HPB45499.1"
                     /translation="MTRYAAIDIGTNSVRLLIGDGVTSDEREMRITRLGRGVDATGKL
                     NPDGIDATLAVLRQYRDIIDRYEIGGLRATATSAARDAANRDEFFGPATEVLGVTPEL
                     LSGDDEARLSFLGAVSGVDATQAPFLVADIGGGSTEFAYGTIIDGAPRFDRGISIDVG
                     CVRMTERFIHSDPPDPAELANCLEVLSMHWDDVRGAIPEASMGPTFIGLAGTVVNVAA
                     VELGLDAYDRNATHHFVLTKDAVEDVFRTLATESLEDRKHNPGLEEARADVIVGGLCI
                     LVSIMRSFGFSECLVSESDILDGLILSQAKR"
     gene            complement(2818..3759)
                     /locus_tag="PLP95_06560"
     CDS             complement(2818..3759)
                     /locus_tag="PLP95_06560"
                     /inference="COORDINATES: protein motif:HMM:NF016314.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF501 domain-containing protein"
                     /protein_id="HPB45500.1"
                     /translation="MSVLAMLFDPAGFDHPVDLAPTDRWGATTVRAAAVTNVLATGLG
                     EVVVVSDRSAHADLRGEVEAFNRIHRRRFASPLTTAAVPPVTVDASRVLVGWLDQPTA
                     APALWHRLHLADLPVVSAGVMTQRAPMALAAPRWDAGAPGVPILGDTVAVELDVWASF
                     QRTPTRDGAYVRECLGREPRAAFTIAARRCDGRPTVIENPPFLDDGTPMPTRFWLVDP
                     DYNRRIGTIEASGAIDVVEAALGLEVLAEAHDRYAAQRDGAIADDHEGPRPTGGVGGT
                     RVGVKCLHTHYAWYLAGGEDPVGAWVHEQLSGGEENE"
BASE COUNT          668 a         1370 c         1238 g          623 t
ORIGIN      
        1 cgggtccgac aggtcgaagc tgatctgctt gccgtaggtc tcgagcttgt gggtgcagtc
       61 gacgtggctg acgaagcaga cgtcgtagtt cgactgatcg agggtttcga ggcggcggcg
      121 cccctcgctg aagtcgctcc gcaacaaccg gcgaggatcc gatccggtga gccacggccg
      181 gagccacgac gagatcgcac ggtcggggcc gccgggtgcg tcgatgacgg tcaccccctc
      241 cggagcgtga tcccgcgtgc ggcgtcgacc gtcgagacac aggaagtcca cctccccgag
      301 ctccaggaat gctccgagga ggttggctaa ccggagccgg tacccgtcgt cggtcggcca
      361 cgggaacgcc tcggctatga cgagcgattt catcgggtca gggtagcggt gggacggcgg
      421 tgccggcgcg ggacatgtga agatgaggct atgtcatttc tctcaatctc ccgccacggc
      481 cgagttgccg tgctcaccct cgacgacccc aagcgccgca acgccctcaa cctcgacctg
      541 aacgctgaac tgatcgaggc catcagcgac atcgaggccg accactcgct cggtgccatc
      601 gtgatcaccg gggccgctcc ggcgttctgt gccggagccg acctcagcca gctcggcgct
      661 tctcagcacg agggcctcaa gcgcatctac gccgggttcc tcgcggtggc gaactgctcg
      721 cttcccacga tcgccgcggt gaacggcgct gcggtcggtg ctggcatgaa cctggccttg
      781 gcctgcgacg tgcgggtcgc tggggcgtcg gcgcgcttcg acacgcgctt tttagatctc
      841 ggcctgcacc ccggtggcgg tcacacctgg atgatgcgcc gtatcgtcgg cccggcggcc
      901 gccgcggcga tggtgctgtt cggccagatt ctcagcggcg aggaggccaa acgcatcggc
      961 ctggcgtggg actgcgtcgc ggacgaggaa ctcgtcgagt actgcatctc gatggcggat
     1021 cgcgccgcga gcgcgccccg cgagttggtc atcaaggtca aggcatcgct cggagccatg
     1081 gcttcgatcg acgaccactc cgaagcggtt gccgccgagc tcgaaccgca ggtgtggtcg
     1141 ctcaatgaac cggcgttcgc cgaacgcctt gccgcgctcc aagcgaagat cacgagtcgc
     1201 tgaccacgct gctccagccg ccgacctgac cacgatcgtg gtcagtaggt ccaggtcgac
     1261 agaagttccg gcccccgctg ttgccactct tcggaggtga tcgcgtagcg aacgtggtcc
     1321 tcccagaccc cgttgatctc caggtaccgc tccgcaatcc cctcgttgcg gaggttgagc
     1381 ttgtccgcga ctcgccgcga ggcggcgttg cgaggcacga tcgacacctg cagccgatga
     1441 agacccatct gctcgaacgc aaactgcatc acgaggacca acgcctcggg catgtacccg
     1501 tgtcccgccc ggcgcacatc gatccagtac ccgacgtacg cgctttgaaa cggaccgcga
     1561 tgcacggagc tgatgttgat ctccccaccg aagctcccgt cgatgaaaat cccgaagctg
     1621 tagccagacc cgagctggat ctcgcggccg cgcgcgccgc atcgcgcggc gaaggcttga
     1681 cggtcctcca cgatgtcggg ggcccccggc acccgtgccg gctcccactt cgtgagccac
     1741 tcctccgaac tcaaccgcac ttcgctccac tggtcgaaat cggcactgct caatggccga
     1801 agcacgaccc gacgaccgat cagcgtgacc ccttgagcga cgggtgcagt ccccaggctt
     1861 ttctgcatcg accggcgagt ccacatcgcg gctgagccta ccgtttggct tggctcagaa
     1921 tgagcccatc gaggatgtcg ctttccgaca ccaggcattc gctgaagccg aagctacgca
     1981 tgatcgacac caggatgcac agccccccga cgatcacatc cgcgcgcgcc tcttccaagc
     2041 ccgggttgtg cttgcgatcc tcaagcgact cggtggcgag agttctgaac acgtcctcga
     2101 cggcgtcttt ggtcaatacg aagtgatggg ttgcgtttcg gtcgtacgca tccaacccca
     2161 actcgaccgc ggcaacgttc accaccgttc ctgccaaccc gatgaacgtc gggcccatcg
     2221 atgcctcggg aatggctcca cgcacgtcgt cccagtgcat cgacaatacc tcgaggcagt
     2281 tcgccaactc ggccggatcg ggcggatcgg agtggatgaa ccgctcggtc atgcggacgc
     2341 agccgacatc gatggagatc cctcggtcga atcgcggtgc cccgtcgatg atcgtgccgt
     2401 acgcgaactc ggtcgacccc ccaccgatgt cggcgacgag aaacggggcc tgcgtcgcgt
     2461 caacgcccga cacggcaccg aggaaactca acctcgcctc gtcgtcaccg ctcaacagct
     2521 ccggcgtcac cccgagcacc tcggtcgccg gaccgaagaa ctcgtcgcgg ttcgcggcat
     2581 cgcgcgccgc cgaggttgcg gtggcacgca aaccgccgat ctcgtaacgg tcgatgatgt
     2641 ctcgatactg ccgaagcacc gcgagcgtgg cgtcgatccc gtccggattg agtttgccgg
     2701 ttgcgtcgac gccgcgaccg aggcgggtga tgcgcatctc gcgctcatcg ctcgtcacgc
     2761 cgtcgccgat gagtaggcgc accgaattgg taccgatgtc gatcgctgcg taacgggtca
     2821 ctcgttctcc tcgccgccac tcaattgttc atgaacccac gcgccgaccg ggtcctcccc
     2881 accggccagg taccacgcat agtgcgtgtg caggcacttc acgccgacac gggtgcctcc
     2941 gacaccaccc gtgggccgcg gcccctcgtg gtcgtcggcg atggcgccat cgcgctgcgc
     3001 ggcataacgg tcgtgagcct cggcgagcac ctcaaggccg agggcagcct ccaccacatc
     3061 gatcgcgccg cttgcctcga tcgtgccgat gcgacggttg tagtcgggat cgaccagcca
     3121 gaaccgcgtc ggcatcgggg tcccgtcgtc aagaaacggc gggttctcaa tcacggtcgg
     3181 gcggccgtcg caacgtcgcg cagcgatggt gaaggcggca cgcggctccc gcccgaggca
     3241 ctcgcgcaca tacgcgccat cccgcgtcgg cgtgcgctga aacgacgccc acacatcgag
     3301 ttcgaccgca acggtgtcgc cgaggatcgg cacccccggc gcaccggcgt cccaccgcgg
     3361 ggccgccagc gccatcggag cacgctgggt catcaccccg gcgctcacca ccggaaggtc
     3421 ggcgaggtgc aggcgatgcc acaacgccgg ggccgcggtc ggctgatcca gccagccaac
     3481 cagaacacga ctcgcgtcga cggtcaccgg tgggaccgcc gccgtggtga gcggcgaggc
     3541 gaaccgacgc cgatggattc gattgaacgc ctcgacctcg ccgcgaaggt cggcgtgggc
     3601 ggatcggtcg ctgacgacga cgacctctcc aaggcccgtg gccaacacgt tcgtgacggc
     3661 ggccgcacgg accgtcgtcg ccccccaccg gtcggtcggc gccaggtcga ccgggtggtc
     3721 gaacccggcc gggtcgaaca gcatcgcgag cacggacatc accacgaccg taccatcgcg
     3781 cccccgaccg gtggactccc cgccgggacg ccgatccgtt cctgtggtcg ggggtgacgc
     3841 gtggcatcac gtccgacagg atgggtccga acggcagcta ccagggagca ccatgaacc
//
LOCUS       DAOOMA010000202         3884 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_28299_length_3884_cov_3.796553, whole genome shotgun sequence.
ACCESSION   DAOOMA010000202 DAOOMA010000000
VERSION     DAOOMA010000202.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 3884)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3884)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3884
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28299_length_3884_cov_3.796553"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..212
                     /locus_tag="PLP95_06565"
     CDS             <1..212
                     /locus_tag="PLP95_06565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPB45501.1"
                     /translation="QGVTVLFVEHDMDVVSAVSDRVVCMAEGRIIADGTAAEVAANEQ
                     VVHAYLGGSSDDLRDMSAERPRQQP"
     gene            209..952
                     /locus_tag="PLP95_06570"
     CDS             209..952
                     /locus_tag="PLP95_06570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPB45502.1"
                     /translation="MTNAPTPQRVVEITNLIAGYVPGVDILRGATMHVDTGEMVCIIG
                     PNGAGKSTLVKAIFGLVPIRSGTVRLNGDEITGAPGHELVSRGVGYVPQVRNVFGTLN
                     VEENLRMGVFLRPGEFHRRRDEIFELFPVLGERRHQRAESLSGGQRQVLAMGRALMME
                     PSLLLLDEPSAGLSPSMQDEVFERVGAINAQGVSVLIVEQNAHKALRICHRGYVLDQG
                     SVAYSGSGPELLHDPMVIELYLGSLARAR"
     gene            complement(1081..2391)
                     /locus_tag="PLP95_06575"
     CDS             complement(1081..2391)
                     /locus_tag="PLP95_06575"
                     /inference="COORDINATES: protein motif:HMM:NF013275.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HPB45503.1"
                     /translation="MKNSRMVRAAMALGACALLGTACGNDDDSASTTTKADSTTTEAP
                     SSGADRGNVDGMLKIGTLVPQTGDLNTIHDSLATPIELALAEINEAGGVLGNDVELVE
                     GDDGTSATVAQTTYDKLINSDKVDLIIGPAPSGVAAKLADGFGTDRVPACSGSTTAAN
                     LQGAGNGYFFRTAPGDDLQGPALAELISGDGHTDVAIIARNDDYGKGFSESLAAALED
                     SGATVTETVLYNPDSGSGYDADVQKALDSSPDAVAVIGFNDDGAQIVSSLIGKGQGPD
                     VMPTYTADGMQGSSFGETVDPSNPGKVAGMKGTAPAAAPAGIDHPFTAKFAATGVDTI
                     FSSYYYDCTILMALAVEAAESDDGTAVKDAFAANLEGDNDCQTFADCKALLAEGKTIH
                     YRGASSAFDTWNVMQPATGVYDVWQFTDDGSVGEVDGAEQISVP"
     gene            2574..>3884
                     /locus_tag="PLP95_06580"
     CDS             2574..>3884
                     /locus_tag="PLP95_06580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012225262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HPB45504.1"
                     /translation="MTTQDSGSTPYPDVAAKPDFAAIEREMLELWDDEGTFQASIDHR
                     DGAPEYIFNDGPPFANGLPHYGHLLTGYVKDVIPRYQTMRGHKVERRFGWDCHGLPAE
                     MQTEKEIDVSGRVAITDFGIGRFNDACRTSVLRYTQEWETYVTRQARWVDFTNDYKTL
                     DLPYMESVMWAFAELHRKGLLYEAYRVMPYSWGAETPLSNFEIRLDDAMRPRQDPALT
                     VRFRLDPAAGDLGDTEMWAWTTTPWTLPANLALVVGPDLDYHLVESPANEAAGGARFV
                     ILGAAAAQRYARELGEDAEVRATMSGRELVGRTYTPLMAYFAGRENCFRVLGDDFVTD
                     SDGTGVVHNAPGHGEDDQRVGEANGIVLVSPVDDSGNYTAEVFDYAGINVFEANPLIA
                     KRLRDESKLVRHDTIDHNYAHCWRTDTPIIYKAVSSWYVEVTAFR"
BASE COUNT          636 a         1313 c         1258 g          677 t
ORIGIN      
        1 cccaaggggt gacggtgctc ttcgtcgaac acgacatgga cgtcgtgtcc gccgtgtccg
       61 atcgggtcgt gtgcatggcc gagggacgca tcatcgcgga cggcaccgcg gccgaggtcg
      121 ccgccaacga acaggtggtc cacgcctacc tcggcggctc gagcgacgac ctgcgcgaca
      181 tgtccgccga acgaccgagg caacagccgt gacgaatgca ccgacaccgc agcgggtcgt
      241 ggagatcacc aacctcatcg ccgggtatgt gcccggggtc gacatcctcc gcggggcgac
      301 gatgcacgtc gataccggcg agatggtgtg catcatcggc cccaacggcg ccggtaagtc
      361 gacgctcgtc aaggcgattt tcggactcgt gcccatccgg tcgggaacgg ttcgcctcaa
      421 cggcgacgag atcaccgggg cgccgggcca cgaactcgtc agccgcggcg tgggctatgt
      481 cccccaggtc cgcaacgtct tcggcaccct caacgtcgag gagaacctgc gcatgggcgt
      541 gttcctgcgc ccgggtgagt tccatcgccg gcgagacgag atcttcgaac tgttccccgt
      601 cctgggcgaa cgtcgccatc agcgcgctga atcgctgtcg ggtggccagc gacaggtcct
      661 cgccatgggg cgtgccctca tgatggagcc ttcgctgttg ctgctcgacg agccctccgc
      721 ggggttgtca ccgtcgatgc aggacgaggt gttcgaacgg gttggcgcca tcaatgcgca
      781 gggcgtttcg gtgctcatcg tcgaacagaa cgcccacaag gcgcttcgca tctgccaccg
      841 cggctacgtg ctcgatcagg ggtccgtggc ctacagcggt tcggggccgg aactgttgca
      901 cgacccgatg gtcatcgagt tgtacctcgg ctcactcgcc cgcgcccgct agcccgctcc
      961 cgcctgacct gcagccctcc ggcagccctc ccgcgcccgc ctgccctgta acggccgttt
     1021 tgtaggtaat tccggcggtc atgaccgccg gaattaccta caaaacggct cgctagaagg
     1081 tcagggaacg gagatctgct ccgcgccgtc aacctcgccg acgctgccgt cgtcggtgaa
     1141 ctgccacacg tcgtagacgc cggtggccgg ctgcatcacg ttccaggtgt cgaacgctga
     1201 ggaggcgccc cggtagtgaa tcgtcttgcc ctcggcgagc aacgccttgc agtcggcgaa
     1261 ggtctgacaa tcgttgtccc cttcgaggtt cgcggcgaac gcgtccttga cggccgttcc
     1321 atcgtcagac tctgcggcct caacggccag ggccatcaag atggtgcagt cgtagtagta
     1381 cgagctgaag atggtatcca ccccggtcgc cgcgaacttc gcggtgaacg ggtggtcgat
     1441 gccagcaggg gccgctgccg gagccgtgcc cttcatgccc gccaccttgc cggggttcga
     1501 cgggtcgacg gtctcgccga agctcgagcc ctgcatgcca tccgcggtgt aggtcggcat
     1561 cacgtcgggg ccctgcccct tgccgatcag cgagctgacg atctgcgcac cgtcgtcgtt
     1621 gaagccgatc acggccacag cgtcggggct cgaatcgagc gccttttgca catctgcgtc
     1681 atagcccgaa cccgagtcgg ggttgtacag caccgtctcg gtgaccgtcg cgccgctgtc
     1741 ttccaacgcc gcggcgagcg actctgaaaa gcccttgccg tagtcgtcgt tgcgggcgat
     1801 gatcgccacg tcggtgtgcc cgtcgccgct gatgagctcc gcgagggccg ggccctgcag
     1861 atcgtcgccg ggtgcggtgc ggaagaagta gccgttgcct gcaccctgaa ggttcgcggc
     1921 ggtggtcgat ccggaacacg ccggcacacg gtcggtgccg aagccgtcgg ccagcttggc
     1981 ggcaacaccc gagggcgccg ggccgatgat gaggtcgacc ttgtcggagt tgatcaactt
     2041 gtcgtaggtc gtctgtgcaa cggtggccga ggtgccgtcg tcgccctcga cgagctcgac
     2101 gtcgttgccc agcacgccgc cggcctcgtt gatctccgcg agcgccaact cgatcggcgt
     2161 tgccagcgaa tcgtggatgg tgttgagatc gccggtctgg ggaacgagcg tcccgatctt
     2221 cagcatcccg tcgacgttgc ctcgatcggc tccgctcgac ggcgcctccg tggtggtgga
     2281 gtcggccttg gtcgtggtcg acgcactgtc gtcgtcgttc ccacacgcgg tgcccagcag
     2341 ggcgcaagcg cccagggcca tcgcggcgcg aaccatgcga ctgttcttca tatgtcctcc
     2401 ctataggtgt tgcacgcact ccgcccggac tgtcgcccag acgatgttcc tgcgtgccgc
     2461 gagaggctag acgctgcgag gccttggatt gcggaacgcc gtcggaacac ctttgcttcg
     2521 acgggccaaa ccggttcggg ccacaccgat cggggccggt agcatcgcgg ctcgtgacca
     2581 cccaggattc cggctccacc ccctaccccg acgtcgctgc gaaacccgat ttcgccgcga
     2641 tcgaacgcga gatgttggag ctgtgggacg acgagggaac atttcaggcg tcgatcgatc
     2701 atcgagacgg ggcgccggag tacatcttca acgacggccc gccgttcgcc aatggtcttc
     2761 cccattacgg ccatctgctc accgggtacg tgaaggacgt catcccccgc tatcagacga
     2821 tgcgtggcca caaggttgag cgtcgattcg gctgggactg ccacggcctg cccgccgaga
     2881 tgcagaccga aaaggagatc gacgtctccg gacgtgtggc catcaccgac ttcggtatcg
     2941 gccgtttcaa cgacgcctgt cgaacctcgg tgctgcgtta cacccaggag tgggagacct
     3001 atgtgacccg ccaggcgcgg tgggtcgact tcaccaacga ctacaagacc ctcgaccttc
     3061 cgtacatgga atcggtcatg tgggcattcg ccgagttgca ccgcaagggc ctgctctatg
     3121 aagcctaccg ggtcatgccg tattcctggg gggccgagac gccactgagc aacttcgaga
     3181 tccgcctcga cgacgccatg cgcccgcgtc aggatcccgc gctcaccgtg cgatttcgcc
     3241 tcgacccggc cgccggcgat cttggcgaca ccgagatgtg ggcgtggacg acgacgccgt
     3301 ggacgctgcc ggccaacctg gcgctcgtcg tcgggcccga cctcgactat cacctggtcg
     3361 agtcgccggc gaacgaggcg gccggcggag cccggttcgt gatcctgggc gccgctgcgg
     3421 cgcaacgcta cgcccgcgaa ctcggcgaag acgccgaggt gcgggccacg atgagcgggc
     3481 gcgaactcgt cgggcgcacc tacacgccgc tgatggcgta tttcgccggg cgcgagaact
     3541 gcttccgggt gctcggcgac gacttcgtca ccgattccga cggcaccggg gtcgtgcaca
     3601 acgcgcccgg ccacggcgag gacgatcagc gcgtcggcga ggcgaacggc atcgtgttgg
     3661 tcagcccggt cgacgactcg ggcaactaca ccgccgaggt cttcgactac gccggcatca
     3721 acgtgttcga ggccaacccg ctcatcgcca aacggctgcg cgacgaatcc aagctcgtgc
     3781 gtcacgacac gatcgaccac aactatgcgc actgttggcg caccgacacc cccatcatct
     3841 acaaggccgt gtcgtcgtgg tacgtcgagg tcaccgcgtt ccgc
//
LOCUS       DAOOMA010000203         3882 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_28320_length_3882_cov_3.614842, whole genome shotgun sequence.
ACCESSION   DAOOMA010000203 DAOOMA010000000
VERSION     DAOOMA010000203.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 3882)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3882)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3882
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28320_length_3882_cov_3.614842"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2427)
                     /locus_tag="PLP95_06585"
     CDS             complement(<1..2427)
                     /locus_tag="PLP95_06585"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HPB45505.1"
                     /translation="MIQFWSLVIAGAVSGGLYAVMASGLVLTYQASGIFNVGQGAVAF
                     ATALTYFQLHQPTEAGGLGWPILPAAIVSILIVAPLLGVALDSLIFRRLSAAPEAARL
                     VGTVGILIALPAAALWLIDVINTIVGSDMANVAGDAGIAPPGIGPTPARTWSLTRGVA
                     INSDQLAVLILAALAAGGLWFLLRHTRFGLETRAAVDRPTLARLRGIDTDRSSRIVWA
                     LSTLLAGCAGVLIAPLFDLSQHTFHTIVFISFTAAVAARLTSVPWAFVAGIALGIIQN
                     LVYGYAPAALTSISGFRSSVPFLLMFVLLFFVQGRGREAGSVAEEAPRPDPRDDLSPW
                     RRRLPWIIAGVLFTFYTVAIATPYWTGILTRGLVFALIFLSFVVVTGIGGMINLAQAT
                     FVTVGGFTVGFLVNHQWPSTVPVLMDNGRIVFWVAVILAVVVTAAVGILVALPSIRLG
                     GLTLALATLALAFVGDRLVFQLEGVRNGSRGWSVRKPVYGPIDLNDDNTLFLVVLVMV
                     LAVVGLIGNLQRSATGRTLLAIRSSAVAAATSGVAPMRAKLMLFGLSAGLAGFAGAWF
                     ALVNSPMTNLSAPPLLGMIWLAVAVTFGIRRPGGAVIAGIVYAALPVVLAGIGGRWTG
                     APWSSIPESVRHLIANPELAALMFGLGAISLAREPDGALAEVATAMRERRRSRAERAA
                     PSPGADSQAATTETARDATETAQKAASGEPDPRLAVQADDAVLVVRNVHAGYGDVAVL
                     HGVDLALRPGSVVAVLGPNGAGKSTLCGVMSGTVAASSGSVDFDGADVTAQAPHHRSG
                     AGLVLAPEARG"
     gene            complement(2471..3727)
                     /locus_tag="PLP95_06590"
     CDS             complement(2471..3727)
                     /locus_tag="PLP95_06590"
                     /inference="COORDINATES: protein motif:HMM:NF024850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HPB45506.1"
                     /translation="MLKRTHRIGAVALSAAMMFMAACSESVDDTASSNTTAKNDSTES
                     TAPQTRTTRGITDTSIKVGGVQYGVYFGDASVGVEARIKEANDAGGVHGRTLEFIGAQ
                     ENNNDATKDLDLVRALVEQDEVFALLPVMAGTFGATDYIVDNNIPTFGYGITPAFCNN
                     EIAFGVTGCVTNPDLTVGSNALGTVLADYFDGDTNKTIAFIGEDNDAGRGGLALLGAS
                     VEDKGFEIVYSEPAMPAPPEQVGDVSPFVSALLAEDPDVIYLQATLSGTKIADALQQA
                     DYDGVIISPSFSPLLLGQPGYEGILINTQVGVDPEIEGLAHLLESVHAIKPDQQLSLA
                     LITGYLAADMFIKGLEETGEDLTVENFLATMNGGFTYSVDGVVGESTWPDNHSKPVPC
                     SVLTKTENAQFVPFQALQCGTNIDVK"
BASE COUNT          694 a         1175 c         1390 g          623 t
ORIGIN      
        1 gccgcgggct tccggagcca gcacgagccc ggcacccgag cggtgatggg gagcctgagc
       61 tgtgacgtca gcaccgtcga aatcaacgct gcccgaactc gcggcgacgg tgccactcat
      121 caccccgcac agggtggact tgccggcccc gtttggtccg agcacggcga cgactgagcc
      181 tgggcgtagc gccaggtcaa cgccgtgcag cactgcgaca tcgccgtatc cggcatggac
      241 gttgcgtacg acgaggacgg cgtcgtcggc ctggactgcc aaacgcgggt cgggctcgcc
      301 actcgcagcc ttctgtgcag tctccgttgc gtcgcgtgct gtctcggttg tcgcggcttg
      361 tgagtcagcg cccggtgagg gtgccgctcg ctccgcccga ctgcgtcggc gttcacgcat
      421 cgcggtggcg acctcggcga gcgcgccgtc gggttcgcgg gccaggctga tggctccgag
      481 gccgaacatc agcgcggcga gttcggggtt ggcgatgagg tgacgtaccg actcgggaat
      541 cgacgaccag ggtgcgccgg tccatcgccc gccgatgccg gcgagtacga ccggcagcgc
      601 tgcgtacacg atgccggcga tcacggctcc gccggggcga cggataccga aggtgacggc
      661 gacggcaagc cagatcatgc cgagcagcgg cggcgcgctg aggttggtca tcgggctgtt
      721 gaccagggcg aaccacgctc cggcgaatcc ggcgagtccg gccgacaacc cgaagagcat
      781 gagctttgcc cgcatgggcg cgacgcccga ggttgcggcg gcaaccgcag aggatcgaat
      841 cgccaacaac gtgcgcccgg tcgcggaacg ctgaaggttg ccgatgagcc cgacgacggc
      901 caggaccatg accagtacga cgaggaacag ggtgttgtcg tcgttgaggt cgatcgggcc
      961 gtagaccggc ttgcgcaccg accagcctcg cgaaccgttg cgaacacctt cgagttggaa
     1021 gacaagacgg tcgccgacga aggcgagtgc gagtgtcgca agggccaacg tcaagccgcc
     1081 gagtcgaatg gacggcaggg cgacgaggat gccgacagcg gccgtcacga cgacggcgag
     1141 gatgaccgca acccagaaca cgatgcgccc gttgtccatc agcactggca cggtggatgg
     1201 ccactggtgg ttgaccagga acccgacggt gaagccgccg acggtgacga aggtggcttg
     1261 ggccaggttg atcatgccgc cgatgccggt gacgacgacg aacgacaaga agatgagggc
     1321 gaacaccagc cctcgggtga ggatcccggt ccagtacggg gtggcgatcg cgaccgtgta
     1381 gaaggtgaac agaacgccgg cgatgatcca ggggagtcgg cggcgccacg gcgagaggtc
     1441 gtcgcgcggg tcgggtcgag gtgcttcctc ggcaaccgat ccggcctccc ggccgcggcc
     1501 ctgcacgaag aacaacagca cgaacatcaa caggaatggc accgaggatc ggaatccgga
     1561 gatcgacgtg agagccgccg gggcgtagcc atagacgagg ttctggatga tgccgagggc
     1621 gataccggcg acgaaggccc acgggaccga ggtcagcctc gccgccaccg ctgcggtgaa
     1681 cgagatgaac acgatcgtgt ggaacgtgtg ttgtgacagg tcgaacaacg gggcgatcaa
     1741 cacccccgcg caaccggcga gcagggtgct gagtgcccag acgattcgcg atgagcggtc
     1801 ggtgtcgatg ccgcggagtc gagccagtgt cggccggtca accgcggcgc gggtctcgag
     1861 gccgaatcgg gtgtggcgca acaggaacca cagaccgccg gccgccaggg cggccaggat
     1921 gaggaccgcg agttggtcgg agttgatggc gaccccgcgg gtgagcgacc aggtgcgagc
     1981 cggagtcggt ccgatgccgg gcggggcgat cccggcatcg ccggcgacgt tggccatgtc
     2041 ggagccgacg atggtgttga tgacgtcgat gagccacaac gctgccgccg gcagggcgat
     2101 cagaatgccg accgtgccga cgagccgggc ggcctcggga gcagcggata gacgccgaaa
     2161 gatgagcgaa tcgagcgcga cgcccagcag cggtgcgacg atcaggatcg acacgatcgc
     2221 cgcgggaagg atcggccagc cgaggccacc cgcctcggtc ggctgatgca gttggaagta
     2281 ggtgagggcg gtggcgaagg ccaccgcccc ctgccctacg ttgaagatgc ccgacgcctg
     2341 ataggtcaac accaagcccg atgccatgac ggcatagagg ccgccggaaa cggcgccggc
     2401 gatgacgagg ctccagaatt ggatcacgtc gcccccttgt ggcggcgggt ggggtcgtcg
     2461 ggaatcacgg tcacttcacg tcgatgttgg tgccgcactg cagtgcttgg aacggcacga
     2521 actgggcgtt ttcggtcttg gtcaagacgc tgcatggaac cggcttggaa tggttgtccg
     2581 gccaggtcga ctcgccgacc acaccgtcga ctgaatacgt gaacccaccg ttcatggttg
     2641 ccaggaagtt ctcgaccgtc aggtcctcac ccgtttcctc gagacccttg atgaacatgt
     2701 cggcagcgag gtatccggtg atcagcgcca ggctcagctg ctgatccggc ttgatggcgt
     2761 gcaccgattc gagcaggtgt gcgagccctt cgatttccgg atcgacgccg acctgggtgt
     2821 tgatcaggat gccctcatat ccgggttgac cgagcagcag cgggctgaac gacggcgaga
     2881 tgatgacacc gtcgtaatcg gcctgttgca gcgcatcggc gatcttcgtt ccggacaggg
     2941 tcgcctgcag gtagatcacg tcggggtctt cggcgagcaa cgctgacacg aacgggctca
     3001 cgtcacccac ctgttccggc ggtgcgggca tcgccggctc ggaatagacg atttcgaatc
     3061 ccttgtcctc gaccgacgcg cccagcaggg ccagaccgcc gcgcccggca tcgttgtcct
     3121 caccgatgaa cgcgatcgtc ttgttggtgt ctccgtcgaa gtagtcggcc agcacggtgc
     3181 cgagcgcatt ggatccgacc gtgaggtccg ggttggtgac acatccggtg acaccgaagg
     3241 cgatctcgtt gttgcagaac gccggcgtga tgccgtaacc gaaggtcggg atgttgttgt
     3301 cgacgatgta gtcggtggcc ccgaacgttc cggccatcac cggcaacagc gcgaagacct
     3361 cgtcttgttc gacgagggcg cgaaccaggt cgaggtcttt ggtcgcgtcg ttgttgttct
     3421 cctgggcccc gatgaactcg agggtgcggc cgtggacccc gccggcgtcg ttggcctcct
     3481 tgatccgggc ttcgacaccg accgatgcgt cgccgaagta cacgccgtac tggacgccgc
     3541 cgaccttgat cgaggtatcg gtgatgccgc gtgtggtgcg cgtctgcggc gctgtggact
     3601 cggtcgagtc gttcttggcg gtcgtgttcg atgatgcggt gtcatcgacc gattccgagc
     3661 aggcggccat gaacatcatc gcggcgctga gtgcgacggc gccgattcgg tgggttcgtt
     3721 tcagcatggg tggtggtctc ctggagtggt cggccgaagg gccgggttgg gtgcctcgcg
     3781 tcggacgttg tgaacgccta caccgaggtt gtggtcccga ggtacgcatc gtgcaccttg
     3841 gggacgttgc ggacttcggc gggaaggccg gcggcaatga gc
//
LOCUS       DAOOMA010000204         3881 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_28335_length_3881_cov_4.189754, whole genome shotgun sequence.
ACCESSION   DAOOMA010000204 DAOOMA010000000
VERSION     DAOOMA010000204.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 3881)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3881)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3881
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28335_length_3881_cov_4.189754"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(215..844)
                     /locus_tag="PLP95_06595"
     CDS             complement(215..844)
                     /locus_tag="PLP95_06595"
                     /inference="COORDINATES: protein motif:HMM:NF015018.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP/GTP-binding protein"
                     /protein_id="HPB45507.1"
                     /translation="MVSRHFDDMGAPNGSEGALSAPHQPMNVPIPVKIVVAGGFGVGK
                     TTFVNSISEIDPLRTEAAMTDRSIGVDEINVATAKSATTVAMDFGRVTLGNDELILYL
                     FGTPGQERFHFMWDELVKGAIGAVVLVDTTRLADSFAAIDYFEQRGLPFVVGVNCFHG
                     AVRHRLEDVRDALALNPAVPLFPTDARARNATQQALIVLVQHAMAAASA"
     gene            complement(825..1196)
                     /locus_tag="PLP95_06600"
     CDS             complement(825..1196)
                     /locus_tag="PLP95_06600"
                     /inference="COORDINATES: protein motif:HMM:NF017170.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF742 domain-containing protein"
                     /protein_id="HPB45508.1"
                     /translation="MTTSPDSFDDRMMRIRPYALTGGRTRPAVELTLETLVRTNDRGW
                     AIRHELSSEERRILDYAANPISIAELSAHLGVVLGVARVLVADLVTSGSLDRSSPATQ
                     AVEAHRDVSLLERVLDGLQAL"
     gene            complement(1193..1615)
                     /locus_tag="PLP95_06605"
     CDS             complement(1193..1615)
                     /locus_tag="PLP95_06605"
                     /inference="COORDINATES: protein motif:HMM:NF015232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="roadblock/LC7 domain-containing protein"
                     /protein_id="HPB45509.1"
                     /translation="MTIISEQAGNVSFLVSNFADRVPGVRDAIVLSADGLLMAMSSGM
                     TRESADRFAAAASGLIGLAHGASAPFGCGRVTEVVIELEHGFMFVTGIGDGSSMAVVS
                     DAGCDVGLVGYEMARLAERCGAVLTPELRAELQGALAR"
     gene            complement(1612..>3881)
                     /locus_tag="PLP95_06610"
     CDS             complement(1612..>3881)
                     /locus_tag="PLP95_06610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB45510.1"
                     /translation="SWYADPSEEGYQAMAALTQDLGGEATLLFDTFTQYADPATADML
                     SAVRASDASRASDPGIAMLSAPPATRAEAVAMPATAAEWATLAAARTDELLTIESHLL
                     DLDYARTAQHVDSADFAVTWFAAAATAATLGVALLAFAISRGINQSLTRLTDAAHTLS
                     SDQVPTLLESLKTPNFDGTSVAMLEVDTGSNDEFAEVGRSLAELSNAIVHVASEQNQQ
                     LRRGISEIFVNLARRNQSLLDRQIQFIDRLETNEEDPDQLENLFRLDHLATRMRRNAE
                     SLLVLAGAEAPRRRARNVEIGDVVRVAIGEVEDFARVELVAVEPSIALGSVAVDIAHL
                     LAELMENGAQYSPPNTTVDVVGRRERNGGYSIVITDHGVGMNESQLASANSVLKQPPP
                     VGLALSRSLGFVVAGTLAQRHCIGVELAVGLDGGVVASVSIPAALLADQPTAVDGPRE
                     ATNAPALGNVVPIRPGSLNTGAAPIPPGKGVPGPLATPPAFTGADAFDAFIAPAEAEA
                     SRVELFDRDALFNRADTFEEEAFEDVDVLEEARSFEDARLSEAAGSLESAEPREPERL
                     LSDAPAPHRLVDILPSGDRFDEGVYGLLGSPVLPSIPAPIAHPGRTLAEGAGTKADHV
                     EPTASRPGPPSSGPAAPTPPVWPAGGGPAWVPPAYDPAGTPPGAAFLPPSVPGAVSGV
                     GAQAPIASAPPLPQRRPGASTPTSSIGGDNNIAAPTRPASEGGSALSRYRNGLVTGRS
                     NPHDGAAPQHQGDHQ"
BASE COUNT          655 a         1270 c         1339 g          617 t
ORIGIN      
        1 gatgcaggca tcggactgat tccagtggac gatgacgacg gtgaccggag cactcatggc
       61 gcgccgagtc tcgcaccacc gtggccctcc ggccaacgtg agggggccgc cgccatcggg
      121 ccacctacgc gaccacgacg tggttctgta ggtgagttcg gttgccagac caaccgaaat
      181 cacctacaga acgagcttgg gcgagggtgg cggactacgc gctcgcggcc gccatcgcgt
      241 gctggaccag cacgatcaac gcctgttgcg tggcgtttcg cgcccgggcg tcggtcggga
      301 acagcggcac cgcggggttc aacgccagcg cgtcccgaac gtcttcaagc cggtgacgaa
      361 cggcgccgtg gaagcagttg acccccacga cgaacggcag gccgcgctgc tcgaaatagt
      421 cgatggcggc gaaggagtcg gcgaggcggg tggtgtcgac cagaacgacc gccccgatgg
      481 cgcctttcac cagctcatcc cacatgaaat ggaaccgctc ctgaccgggt gtgccgaaca
      541 gatacaggat cagctcatcg ttgccgaggg tcacccgacc gaagtccatc gccaccgtcg
      601 tcgccgactt ggcggttgca acgttgattt cgtcgacgcc gatgctgcgg tcggtcatgg
      661 cggcctcggt gcgcagcgga tcgatctcgg agatcgagtt cacgaacgtg gtcttgccga
      721 cgccgaaccc gccggcgacc acgatcttca ccgggatcgg aacgttcatc ggttgatgcg
      781 gggccgacag tgccccctcg gacccgttgg gcgcacccat gtcgtcaaag tgcctggaga
      841 ccatcgagga ccctttcgag aagcgagacg tcgcgatgcg cctcgaccgc ctgagtggct
      901 ggagaacttc gatcgagtga acccgaagtg acgagatcgg caacgagcac ccgtgcgacg
      961 cccaatacga cgccgaggtg cgctgacagc tcagcgatcg agatggggtt cgcggcgtag
     1021 tcgaggatgc gtcgctcctc ggaactgagc tcgtggcgaa tggcccaacc ccggtcattg
     1081 gtccgcacga gggtctcaag ggtcaattcg accgctggac gcgttcgacc tccggtcaac
     1141 gcgtacggcc ggatgcgcat catccggtcg tcaaacgagt cgggcgaggt cgtcaccggg
     1201 cgagcgctcc ctgcaactcg gcgcgcagct cgggggtcaa caccgcgccg cagcgctcgg
     1261 ccagacgcgc catctcatag cccaccagac ccacgtcgca accagcatcg gaaacgaccg
     1321 ccatcgaact gccgtcgccg atcccggtca cgaacatgaa accgtgttcg agttcgatca
     1381 cgacctcggt cacccggccg cacccaaacg gcgccgacgc accatgggcg agcccgatga
     1441 gtcccgaggc tgccgcggcg aaacggtcgg cggactcgcg tgtcatcccc gaactcatcg
     1501 ccatgagcaa cccgtcggcg gacagcacga tcgcgtcgcg cacgcccggc acccggtcgg
     1561 cgaaattcga cacgagaaag ctcacattgc cggcttgttc gctgatgatc gtcattgatg
     1621 atctccttga tgctgcggcg cggcgccgtc atgcgggttg gaacggcccg tcaccaggcc
     1681 attgcggtac cgggagagcg ctgagcctcc ctcgctcgcc ggtcgagtcg gcgctgcgat
     1741 gttgttgtcg ccgccgatgc tggacgtcgg ggtcgatgcg ccggggcgac gttgcggcag
     1801 cggcggggcg gaggcgatcg gcgcctgcgc accgacgccg ctgacggctc ccggcaccga
     1861 cggcggaaga aacgccgcgc caggcggggt tcccgcggga tcgtaagcag gaggaaccca
     1921 cgcgggtccc ccacctgcgg gccacaccgg tggtgtcggt gcggctggcc cgctcgaggg
     1981 cggtcctggc cgggaggccg tcggctcgac gtggtcagcc ttcgtgccgg caccttcggc
     2041 cagcgtcctg cccggatggg cgatcggcgc ggggatcgac ggaagcaccg gagaaccgag
     2101 cagcccataa acgccttcgt cgaatcgatc gccgctcggc agaatgtcga cgaggcgatg
     2161 cggagctggg gcatcggaca gcagccgttc cggttcgcgg ggttcggcgc tctcgagcga
     2221 gccggccgct tccgacaacc gtgcatcttc aaacgatcgt gcttcttcga ggacgtcgac
     2281 atcctcgaag gcctcctcct cgaaggtgtc ggcacggttg aacaacgcat cgcgatcgaa
     2341 caactcgacc cgcgaagcct cggcctcggc aggagcgatg aacgcatcga acgcgtccgc
     2401 cccggtgaag gcaggcgggg ttgccaatgg cccgggcacg ccctttcccg gagggatggg
     2461 tgcggcgccg gtgttgagcg atcccggacg gatcggcacc acgtttccca aagccggcgc
     2521 gttcgttgct tcacgtggtc catccaccgc ggtcggctgg tccgccaaca gtgcggcggg
     2581 gatcgacacc gacgcgacca ctcccccgtc gaggccgacg gcgagttcca ccccgatgca
     2641 gtgacgctgg gccaacgtgc cagcgacgac gaatccgagc gaccgcgaca gcgccaggcc
     2701 gaccggaggc ggttgcttca gcacgctgtt ggcggacgcc agctggcttt cgttcatgcc
     2761 gaccccgtgg tcggtgatca cgatcgaata gccgccgttg cgttcgcgtc gacccaccac
     2821 gtccacggtg gtgttcggcg gggagtactg cgcaccgttc tccatgagtt cggcgagcag
     2881 gtgggcgatg tcgaccgcga ccgacccgag cgcgatggag ggttcgacgg ccacgagctc
     2941 gacgcgagcg aagtcttcca cctcgccgat ggcgactcgc acgacgtcgc cgatctcgac
     3001 gttgcgggca cgtcggcgcg gggcctccgc cccggcgagc acgagcaggg attcggcgtt
     3061 tcgccgcatg cgggtggcga ggtgatcgag tcggaacagg ttctcgagct gatccgggtc
     3121 ctcctcgttg gtttcgaggc gatcgatgaa ctggatctgg cgatcgagca gggactggtt
     3181 gcggcgggcg aggttgacga agatctcgct aataccacgc cgcagctgct ggttctgttc
     3241 acttgcgacg tgaacgatgg cgttgctcag ttccgcgagg gaccttccga cctccgcgaa
     3301 ctcgtcgttc gaaccggtgt cgacctcgag catggccacc gaggtgccgt cgaagttggg
     3361 ggtcttgagg ctttcgagca gcgtcggcac ctggtcgctg gacagcgtgt gagcggcatc
     3421 ggtcaggcgg gtgagcgact ggttgattcc gcgggagatg gcgaacgcca acagggcgac
     3481 gccgagggtc gccgcggtgg ctgccgcggc gaaccacgtg acggcgaaat cggccgaatc
     3541 gacgtgttgg gccgttcgcg catagtcgag atcgaggagg tgcgactcga tcgtgagcaa
     3601 ctcgtcggta cgtgcggcgg ccagcgtcgc ccattccgcc gccgtcgccg gcatggccac
     3661 tgcctcggcg cgggttgcgg gcggcgcgct caacatggcg attcccgggt cggaggcgcg
     3721 cgaggcgtcg ctggcgcgaa cggcgctcaa catgtcggcg gtcgctgggt cggcatattg
     3781 ggtgaaggtg tcgaacaaca gcgtcgcctc gccgccgagg tcctgggtca gcgccgccat
     3841 cgcctgatat ccctcctccg aggggtcggc gtaccacgac a
//
LOCUS       DAOOMA010000205         3848 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_28636_length_3848_cov_3.718956, whole genome shotgun sequence.
ACCESSION   DAOOMA010000205 DAOOMA010000000
VERSION     DAOOMA010000205.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 3848)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3848)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3848
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28636_length_3848_cov_3.718956"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..697
                     /locus_tag="PLP95_06615"
     CDS             <1..697
                     /locus_tag="PLP95_06615"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF024970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HPB45511.1"
                     /translation="VMLVRDVTEVRHRDRLLLSKDATIAEIHHRVKNNLQTISSLLRL
                     QGRRLSEPTARDAVEESVRRIHSIALVHETLSREPGDDVEFRSLLGPLVRMVNEGLVS
                     SEHPIEFRISGDGGVVASEVATSLSLVVTELLQNAVEHAFVPERQPWHEPLVEIELAR
                     DDDDIVVTVIDNGVGWVPAAVRPARSSESLGQSIIAALVIDELRGTIAYRCGDDGSGT
                     KVVISVSATKRWE"
     gene            complement(873..1163)
                     /locus_tag="PLP95_06620"
     CDS             complement(873..1163)
                     /locus_tag="PLP95_06620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WhiB family transcriptional regulator"
                     /protein_id="HPB45512.1"
                     /translation="MALTSSRSLTLANDDWRTNAACQDTDPDLFFPVGTTGPAIEQID
                     NAKRVCSTCEAKDPCLEFAMMTNQDSGVWGGTSEEERRKLRRAWLAAQRKSA"
     gene            1376..2116
                     /locus_tag="PLP95_06625"
     CDS             1376..2116
                     /locus_tag="PLP95_06625"
                     /inference="COORDINATES: protein motif:HMM:NF015000.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HPB45513.1"
                     /translation="MIGHRGASHDYPENTLEAFRGAAAQGADWVELDVRRTRDGLLVV
                     HHDPHLSDGRTIAEMTSREIPDSVPSLAAALETCAPMGVNVEIKNTEGEPGHDATAGI
                     AREVVKTIRATVKSVTDILISSFDLPTLDAARHADGDIATGFLVLSTEEPADAVALCV
                     ERGHQAANPYDLFVSAESVARAHQSGVAVNVWTVDDPGRIAQLASWGVDAVITNRPDV
                     ALGALGRPRSRGLDPVLGEGSCGEPEST"
     gene            complement(2225..3154)
                     /locus_tag="PLP95_06630"
     CDS             complement(2225..3154)
                     /locus_tag="PLP95_06630"
                     /inference="COORDINATES: protein motif:HMM:NF012981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HPB45514.1"
                     /translation="MKVLLIVNPRASSVRPRRQLGVHRLLGTEHTVQMVETTHRGHAT
                     QLAADAAHDGVDVVAVLAGDGTLNEAANALIGSTTALMALPGGSTNVFARTLGLSDNP
                     LKATRTALSALRAGSIRSIGTGSANGRHFLFNCGVGWDAVLVRQTERRSHLKRRLGHL
                     LFVWCGLETWFRLYDRTRPHFSVHHEDGRTVDDAYFTVAQNSNPYTYVGHRPFNVSPH
                     LTLVDPLAVTTLTELRARSFLGLMFATLRSRDALATHPIVDHVAKTNGFTITAVDPVA
                     YQADGDDLGDANFIEFRHHPDSLRVVIPEEYPW"
     gene            3260..>3848
                     /locus_tag="PLP95_06635"
     CDS             3260..>3848
                     /locus_tag="PLP95_06635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HPB45515.1"
                     /translation="MNVVVCVKQIPDPADPGALDPSTKTLVRDGKLILDESDAYGVEM
                     ALQLAGDGEVSLVSMAPNGEVSGLRTALAMGAAKAVLVSDDALKGSDALTTAKVLAKA
                     IERVGGYDLVIAGIESSAGYTGVVPAQIAAVLGIPSVTFAKSVTVDGSNIKVDRQTEL
                     GYDEVVAPLPALVAVTAGVVEPRYPSFKGIMAAKSK"
BASE COUNT          645 a         1190 c         1330 g          683 t
ORIGIN      
        1 cgtcatgttg gtgcgcgacg tcaccgaggt gcgccaccgc gaccggttgt tgctcagcaa
       61 ggacgccacg atcgcggaga ttcaccatcg ggtgaagaac aacctgcaga cgatctcgtc
      121 gttgctgcgg cttcagggcc ggcgcctcag cgagccgaca gcgcgagacg cggtcgagga
      181 gtcggtgcga cggatccatt cgatcgcgct cgttcatgag acgctgtctc gcgagcccgg
      241 cgacgacgtc gagtttcgct cgctgctggg ccccctggtg cgcatggtga acgagggcct
      301 cgtcagcagc gagcacccga tcgagtttcg gatctccgga gacgggggag tggtggcctc
      361 agaagtggcg acgtcgttgt cgctggtcgt caccgaactc ctgcagaacg ccgtcgaaca
      421 cgcctttgtc cccgagcgtc agccgtggca cgagccgttg gtcgaaatcg aactggcccg
      481 agacgacgac gacatcgtcg tgacggtgat cgacaacggg gtgggctggg tgcccgctgc
      541 ggtgcgcccg gcgcgcagtt ctgagtcgct ggggcagagc atcatcgccg cgctggtgat
      601 cgatgagttg cgcggcacga tcgcctacag atgcggagac gatggatcgg ggacgaaggt
      661 tgtcatctcg gtctcggcga ccaagcgttg ggagtgactc gcctacgacg aggccatggg
      721 cccggacgcg acgatgggtc gacacgggtc gacccatcgt cggcactgag cgctcaggcg
      781 ctcagcggtt caattgttgc gccacccggg tctcgggtgt tcggtgcaga cgtgttcggt
      841 gctgcagcgt tcggtgctgc agcgttcggt gatcaggcgg acttgcgctg ggcggcgagc
      901 catgcgcggc ggagcttgcg gcgctcctct tcggaggttc cgccccagac accggagtcc
      961 tggttcgtca tcatcgcgaa ctcgaggcac gggtcttttg cctcacaggt cgaacagacg
     1021 cgcttggcgt tgtcgatctg ttcgatggcc gggccggtgg tgccgacggg gaagaagagg
     1081 tccggatcgg tgtcctggca ggcggcgttg gtgcgccagt cgtcgttggc cagggtgaga
     1141 gagcggctcg aggtcagtgc cacgattcct ccgatatgcg tgcggggacg tagatttgaa
     1201 tgcgaattct cggtgtgcca ccgctccaag gccggcatca ggtgatggcc ggatcgtgga
     1261 tcccaagctg cccgccatgt cggtcggact cttgtgaaaa gtggaacaag ggaaagttac
     1321 cgcccgattt ccgggctgta aaggaaaagt ttcgaggtga tttgcgttga cgctcgtgat
     1381 cggccatagg ggtgcctccc acgattatcc ggagaacacc ctggaggcgt ttcgtggcgc
     1441 cgcggcccaa ggtgccgatt gggtggaatt ggacgtgcgt cggactcggg acggactcct
     1501 ggtcgttcat cacgaccccc acctgagcga cggccgaacg attgcagaga tgacgtcacg
     1561 agagattccc gactcggtac cttcgctcgc ggcggccttg gagacatgcg ctccgatggg
     1621 cgtgaacgtc gagatcaaga acaccgaagg ggaacccggc catgacgcta ccgccggaat
     1681 cgcccgcgag gtcgtcaaaa cgattcgggc caccgtgaaa agcgtcaccg acatcctcat
     1741 cagcagcttc gacctcccaa ccctggacgc agcgcggcac gccgacggcg atatcgccac
     1801 ggggtttctc gtgttgtcga ccgaggaacc cgccgatgcg gtcgcgctgt gtgtcgagcg
     1861 cggccaccag gcggcgaatc cctacgacct gttcgtgtcg gccgagtcgg tcgcccgagc
     1921 ccaccaatcc ggtgtcgccg tcaacgtctg gaccgtcgat gaccccggtc gcatcgcgca
     1981 gctcgcctcc tggggcgtcg acgccgtgat cacgaatcgt ccggacgtgg cgctcggggc
     2041 gcttggtcgg cctcggagtc gtggcctcga ccccgtcctc ggcgaagggt cgtgcggcga
     2101 gcctgaatca acgtgaacat cacggtcagg gcggcgacga tcgccaacgc ccaccatcgc
     2161 gaggcccctg cactcgcgcc gcagccgttc tctccgcagg actccacaac tgcggcgatc
     2221 accgtcacca tgggtactcc tcgggaatca cgacccgcag cgagtcgggg tggtggcgga
     2281 attcgatgaa gttggcgtcg ccgaggtcgt cgccatccgc ctgatacgcg actggatcga
     2341 ccgcagtgat ggtgaatccg ttggtcttgg ccacgtgatc gacgatcgga tgggtggcga
     2401 gcgcgtcccg tgagcgcaac gtggcgaaca tcaatccgag aaacgagcgg gcccgcaact
     2461 cggtgagggt cgtcaccgcg agcgggtcga cgagggtgag gtgcggcgag acgttgaacg
     2521 ggcgatgccc gacgtaggtg taggggttgg agttctgtgc gacggtgaaa taggcgtcgt
     2581 cgacggtgcg cccgtcctcg tggtgtaccg agaaatgtgg ccgcgtccgg tcatagagcc
     2641 ggaaccacgt ctcgaggccg caccacacga acaacaggtg gccgaggcgg cgcttgaggt
     2701 gcgagcgccg ttcggtttgg cgtaccaaca cggcgtccca gccgacgccg cagttgaaca
     2761 ggaagtggcg cccgttcgcc gaaccggtgc cgatggatcg aatcgaaccc gcgcgcaggg
     2821 cgctgagcgc ggtcctggtc gccttgagcg gattgtcgct gaggcccaac gtgcgagcga
     2881 acacgttggt ggaacccccg ggcagggcca tgagtgcggt ggtcgagccg atcagggcgt
     2941 tggcggcctc gttcaaggtg ccgtccccgg cgagcaccgc cacgacatcg accccgtcgt
     3001 gcgccgcatc ggccgcgagt tgggtggcgt ggccgcggtg ggtggtttcg accatctgga
     3061 ccgtgtgttc ggtgccgagg aggcgatgca caccgagctg tcggcgcggt cgcaccgacg
     3121 aggcgcgggg gttgacgatc aacaacacct tcatgacgtt cgtaccgtag cccgggcacc
     3181 tcggcgcggc ggaagtgggg tggccgaccc tcgatatccg aggttgtttg tgaatacatt
     3241 ctcttggcag actgtgacca tgaacgtcgt tgtttgtgtc aagcagatcc cagacccggc
     3301 tgatccgggc gcgctggatc cctccacgaa gaccctcgtg cgcgatggca agctcatctt
     3361 ggatgagtcc gatgcctacg gcgtcgagat ggccctgcag ctcgccggcg acggcgaggt
     3421 ctcgctggtg tcgatggccc cgaacggtga ggtctccggc cttcgtaccg cactcgccat
     3481 gggagccgcc aaggccgtcc tcgtcagcga cgatgccttg aagggttcgg atgccctgac
     3541 caccgccaag gtgctcgcca aggccatcga gcgggtcggc ggctatgacc tggtcatcgc
     3601 cggtatcgaa tcatccgccg gctacaccgg tgtcgttccg gcgcagatcg cggccgtgct
     3661 cggcattccc tcggtcacct tcgccaagtc cgtcacggtc gatggctcca acatcaaggt
     3721 cgatcgccag accgaactcg gctacgacga ggtcgtcgca ccgcttccgg cgctcgtcgc
     3781 cgtgaccgcg ggcgtggtcg agccccgcta cccctcgttc aagggcatca tggccgccaa
     3841 gtccaagc
//
LOCUS       DAOOMA010000206         3833 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_28794_length_3833_cov_3.604817, whole genome shotgun sequence.
ACCESSION   DAOOMA010000206 DAOOMA010000000
VERSION     DAOOMA010000206.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 3833)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3833)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3833
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28794_length_3833_cov_3.604817"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..778)
                     /locus_tag="PLP95_06640"
     CDS             complement(<1..778)
                     /locus_tag="PLP95_06640"
                     /inference="COORDINATES: protein motif:HMM:NF012415.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HPB45516.1"
                     /translation="MRFRNLAVTASLALFAIGCTPDSGSGSTVTTTSTATATSTTSTT
                     TTSTTSSTTTTTIPSTGTINRQDRASVQSAYVNRLLPALAAPSGWTGNVATCNAGTTT
                     TAHQTATLATVNFFREFVGLTPVTFNSTYSTKAQQAALMMQANNSLSHTPPSTWTCYS
                     ADGAAAAGSSNIALGTTNGPEAIRLYIADPGTNNTAAGHRRWILDPTRVTMGSGTTGN
                     ANALWVFGTTQALPSTPAVVAWPADGFFPVELEPQGRWSLS"
     gene            897..1721
                     /locus_tag="PLP95_06645"
     CDS             897..1721
                     /locus_tag="PLP95_06645"
                     /inference="COORDINATES: protein motif:HMM:NF014035.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HPB45517.1"
                     /translation="MNPLVFVLCALALSALGGLVGSLVGLGGGIVVVPGLTLLLGVDI
                     KVAIAASMIAVIATSSGSAVAYTRDRMSNLRVAMFLEVSTVCGGICGALLVGIANPSL
                     LNGFFAAVLLIAAATMMRRRHDASAGPPSALADRLELHGSYPDARTGREHPYRVSRPA
                     VGFAIMYFAGVASGMLGIGGGVFKVPAMDLAMRLPIKVSTATSNLMIGVTGTAAALIY
                     LLRGDVNPVVAAPVAIGVLGGANLGARLMPRVHDGVLRRLFVVVMVLTAVQMAAKI"
     gene            1718..2179
                     /locus_tag="PLP95_06650"
     CDS             1718..2179
                     /locus_tag="PLP95_06650"
                     /inference="COORDINATES: protein motif:HMM:NF019459.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1634 domain-containing protein"
                     /protein_id="HPB45518.1"
                     /translation="MSGQDMSADRSLEAADDGAPSDSSDPGDASGGLEVVISKVLRLG
                     VVASLTVMTIGVVVGFVDGSLSGDRGALDRLIDPNLAASHRLDAAWESLRSGGAVGIM
                     TLGLCLLVATPLVRVVASLVGFARMGDRIYLVLTSAVVALLALSCALGVAG"
     gene            complement(2110..3483)
                     /locus_tag="PLP95_06655"
     CDS             complement(2110..3483)
                     /locus_tag="PLP95_06655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB45519.1"
                     /translation="MRVERYAVVGLARVRSPWFGEVASWANAGVLPIDFIKCVSTAEL
                     RARLHDARGVSAILVEAGVAGFDRDLIDEARTLGVASIVVGAASELGHETDWARLGAA
                     AVLPDRFDEATLIDTLSNVAEPISSAPAVVVDSESLDDRDQDASSPGSLIAVIGSAGS
                     GTSTTAMALAQGLARQTANASEGRLGSVVLADLVRRGHLAMYHDAKDVVPGFQELVES
                     HRNASPTPREVAEHLFDVDDRGYALLLGLRRTRDWTALRPKAVEASIESLRSNFDHVV
                     ADLDAELDGEAETGSVDVEERNAASRLVVRDASLIVITTDTSLKSVHEFLHLLRDLAD
                     HGVCASRILPVVTRAPRSPRQRAECTRTLSTLGGLPGDLSPVFVPAHRHLEAIHANGS
                     PLPQAVCSPLVGAVRAALCMGTATARTSRTVDPPGSDDSAKTAHPATPRAHDSANNAT
                     TALVSTR"
     gene            complement(3480..>3833)
                     /locus_tag="PLP95_06660"
     CDS             complement(3480..>3833)
                     /locus_tag="PLP95_06660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB45520.1"
                     /translation="LASAISEPLGTDANTGRAIGLTLDAARALGGNLSVGDRIDIYAL
                     GSSGSDAEVLERNARITAVRGTTGESIGNSASVGFTVAVSDEAAALRIVTAESTVGLT
                     VVKGSPHPADPESAR"
BASE COUNT          547 a         1194 c         1399 g          693 t
ORIGIN      
        1 tcgacaacga ccaccggccc tgcggctcga gttcgaccgg gaagaacccg tcggcgggcc
       61 atgcgacgac agcgggcgtc gacggcaacg cttgggttgt gccgaacacc cacagggcgt
      121 tggcattgcc ggtcgtgccc gaacccatcg tcactcgcgt cgggtcgagg atccagcggc
      181 ggtgacccgc tgcggtgttg ttcgtccccg ggtcggcgat gtagaggcgg atggcttcgg
      241 ggccatttgt tgtgccgagc gcgatgttgg agctgccagc ggccgcggcg ccatccgcgc
      301 tgtaacaggt ccacgtcgag ggcggggtgt ggctgagcga gttgttggcc tgcatcatga
      361 gtgcggcctg ctgggccttt gtcgagtagg tgctgttgaa ggtcaccgga gtcaggccga
      421 caaactcgcg gaagaagttg accgtcgcga gggtcgccgt ctgatgtgct gtcgtggtgg
      481 ttccggcatt gcaggttgcg acgttgcccg tccagccgct gggtgccgcg agcgccggca
      541 acaatcggtt gacgtacgcg ctctgcaccg aggcccggtc ttggcgattg atcgtccccg
      601 tcgacggaat cgtggtggtc gtcgttgagc tcgtggtcga ggtggtggtc gtggacgttg
      661 tcgaggtggc cgtggcggtc gaggttgtcg tcaccgtgga accgctgccg ctatcggggg
      721 tgcacccgat agcgaaaagc gcaaggctgg ccgtgaccgc aaggttccgg aatcgcatgc
      781 ttccccctca tagggtcgga ctccttggat gctagcggcg gcatcggatt ttgcgggcgg
      841 tgcccacaca cggtcgacga ggagaccccg atggacggtg ggtagggtgg gcagccgtga
      901 acccgctcgt gttcgtgctc tgcgcgctcg ccctgtccgc gctcggcggt ctggtcggct
      961 cgctggtggg gctcggcggg ggcatcgtcg tggtgcccgg gctgaccctg ctgttgggag
     1021 tcgacatcaa ggtcgccatc gcggcgtcga tgatcgcggt gatcgccacc tcatctggct
     1081 ccgcggtggc ctacacccgc gaccgaatga gcaatctgcg ggtggcgatg tttctggaag
     1141 tgtcgacggt gtgtggcggt atctgcggtg cgctgctcgt ggggatcgcc aacccgtcgc
     1201 tgctcaacgg cttcttcgcc gccgtgttgt tgatcgcggc cgcgacgatg atgcggcgac
     1261 gccacgacgc ctctgcagga ccgccgagcg cgctcgcgga tcgcctcgaa ctgcacggca
     1321 gctaccccga cgcccgcacc ggacgcgagc acccctaccg ggtgagtcgg cccgcggtcg
     1381 ggttcgccat catgtacttc gcgggggtcg catcgggcat gttgggtatc ggcggcgggg
     1441 tgttcaaggt gccggcgatg gatctggcga tgcgcctgcc gatcaaagtg tcgaccgcga
     1501 cctcgaacct catgatcggg gtcaccggca ccgcggccgc gctgatctac ctgttgcgcg
     1561 gcgacgtcaa tccggtcgtc gcggcgccgg tggcgatcgg ggtgctcggc ggcgcgaacc
     1621 ttggagcccg gctcatgccc cgggtgcacg acggcgtgtt acggaggctc ttcgtcgtgg
     1681 tgatggtgct gaccgcagtg cagatggcgg ccaagatatg agcggccaag atatgagcgc
     1741 cgatcgctcg ctggaagcag ccgacgacgg cgcacccagc gattccagcg atccaggcga
     1801 cgcctccggc gggctcgaag tggtcatttc caaggtgctt cgcctcggcg tggtggcctc
     1861 gctgacggtg atgacgatcg gggtggtggt cggcttcgtc gacgggtcgt tgtccggcga
     1921 tcgtggcgcg ctcgaccgac tgatcgaccc gaacctcgca gcatcgcatc gactcgatgc
     1981 cgcctgggag tcgctcagaa gtgggggcgc ggtcggcatc atgacgctgg ggctgtgcct
     2041 gttggtggcc acgccgctcg tgcgcgtcgt ggcctcgctg gtcggtttcg cccggatggg
     2101 cgatcggatc tacctcgtgc tgacgagtgc ggtcgtcgcg ttgttggcgc tgtcgtgtgc
     2161 cctcggggtt gccggatgag cggtcttcgc cgagtcgtca gatcccggtg ggtctacggt
     2221 gcgggatgtc cgtgcggtcg cggtgcccat gcagagcgcg gcgcgcaccg cacccacgag
     2281 tggcgagcac acggcttgtg gcagcggcga tccgttggcg tggatggcct cgaggtggcg
     2341 gtgggccggg acgaagacgg gggacaggtc gccgggcagc ccgccgaggg tcgacagggt
     2401 tctggtgcat tcggcgcgtt ggcgcggcga tcgcggggcg cgggtcacca ccggaaggat
     2461 gcggctcgcg caaactccgt gatcggccag gtcgcgcagc agatgcagga actcgtgcac
     2521 gctcttgagc gaggtatcgg tggtgatcac gatcagcgag gcgtcgcgaa ccacgagcct
     2581 gctcgcggcg ttgcgttcct cgacatcgac cgaaccggtc tcggcttcgc cgtcgagttc
     2641 agcgtcgagg tcggccacga cgtggtcgaa attcgatcgc agtgactcga tggacgcttc
     2701 gaccgccttg ggtcgaagcg cggtccaatc tctggtgcgg cgtaacccca gcagcaacgc
     2761 atagccgcgg tcgtcgacat cgaagaggtg ctcggctact tctctcggtg ttggcgaggc
     2821 attgcgatgg gactcgacga gttcctggaa cccgggcacg acgtctttcg cgtcgtggta
     2881 catggcgagg tgcccgcggc gcacgaggtc ggccaacacg accgatccca gtcggccctc
     2941 cgaggcgttc gccgtctggc gggcgaggcc ctgagccagt gccatcgcag tggtcgacgt
     3001 gcccgacccg gccgaaccga tgacggcgat cagtgaaccc ggagacgaag cgtcttggtc
     3061 gcggtcgtcg agcgactcgc tgtcgacgac gaccgcgggg gcggaactga tcggctcggc
     3121 cacgttcgac agggtgtcga tcaaggtggc ttcgtcgaat cggtccggca gtaccgccgc
     3181 tgcgccgagg cgtgcccagt cggtttcgtg gccgagctcc gatgccgccc cgaccacgat
     3241 ggaggccacc cccaaggtgc gtgcctcgtc gatgagatcg cgatcgaatc ctgcaactcc
     3301 ggcctcgacc agaattgccg agaccccgcg tgcatcatga agccgagccc ggagctctgc
     3361 ggtgctgacg cacttgatga agtcgatcgg cagcaccccg gcgttcgccc acgaggcgac
     3421 ctcgccgaac cacggggagc gaacccgtgc gagtccgacg acggcgtacc gttcgaccct
     3481 catcgtgcgg actccgggtc ggccgggtgg ggtgagcctt tgaccaccgt cagcccgacg
     3541 gtgctttctg cggtcacgat gcgaagcgcg gccgcctcat cggagaccgc aaccgtgaag
     3601 ccgacactcg ccgaatttcc gatcgactcg ccggtggttc cgcggaccgc ggtgatccgg
     3661 gcattgcgct cgagcacctc ggcgtcgctg ccggaactgc cgagggcata gatgtcgatc
     3721 cggtcgccga ccgacaggtt ccctccgagg gcccgagcgg catcgagggt gaggccgatc
     3781 gctcgtccgg tgttggcatc ggtgccgagc ggttcgctga tcgcgctcgc cag
//
LOCUS       DAOOMA010000207         3829 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_28835_length_3829_cov_4.382883, whole genome shotgun sequence.
ACCESSION   DAOOMA010000207 DAOOMA010000000
VERSION     DAOOMA010000207.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 3829)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3829)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3829
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28835_length_3829_cov_4.382883"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1994)
                     /locus_tag="PLP95_06665"
     CDS             complement(<1..1994)
                     /locus_tag="PLP95_06665"
                     /inference="COORDINATES: protein motif:HMM:NF013256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB45521.1"
                     /translation="MHDDRERVEARLRRMVLERVEPARICARVPLEVARWEVPGEPVG
                     VAEAMAARFGAREVGSSFGKPWGTTWFTLAGAVPNEWAGGVVRAAIDLGFSNRPGFQS
                     EGLFWTRTAEGWQPLRGLNPLNHELPITDAASGGESVELALEAASNPDLSVAVPNPAS
                     YLATAGSASLYRLSRAELVLVDPQVEALRHDVRALNGLMKQLDWGDPRRHEILRALET
                     MLDRLDLADAASIARTAPAGRAELAAVLSAPATASAHRITAIGHAHIDTAWLWPLRET
                     RRKCARTFTNQLALIERYPQHRFACSQALHYDWMREHYPTVFRDIGAAVAEGTWIPVG
                     RMWVEADANLSGGEALIRQFTHGQRFFEEHFGVECTEGWLPDVFGYNANLPQILRHVG
                     IERFVTQKLSWNTTNRFPHHSFWWEGIDGSTVFTHFPPVETYNANFSGHELAHAVRTF
                     ADKGRSTRSLMPFGHGDGGGGPTADMLEQFLRVRDLEGSPLVEIGAPSSMLDAAIEEY
                     RDAPRWVGELYLEMHRGTYTSQAATKAGNRRCEALLREAEVWSVAAYGGDAGRGYPAV
                     ALDRLWKSLLTLQFHDILPGSSIGWVHREAQESYSSIELEATELIHTALTRLAPVASE
                     AIVANPTPFAVHAVVPVRRRAGTVEEWPLSLEPHGVSTRS"
     gene            2090..3403
                     /locus_tag="PLP95_06670"
     CDS             2090..3403
                     /locus_tag="PLP95_06670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HPB45522.1"
                     /translation="MSDVDNEAENEEARFEGVFRHFRKGGLGNDAAWGSLHDLTQLFV
                     QTASFLLLGRKLGVEDYGRYVGVYGIVGPIGGLTWSGVTLAVLQRRLGERDSINKTAR
                     DFFLLAIVLGVFATSLGTLIGSQIISGLAVTTILAVMTAELVANAFNQVSLSMVQAEH
                     GFASATRLKMVVLFIRVSVLIALVAVDSLTIGHLAVGFTIGFFLYTALLVRTVLPAFG
                     IRVQLARPSNGILRVTGSISLPIAAGVLQQDGDKAVLNAYNFQHDAGLYGAAFRVVSM
                     GLMPLRALEAAAFQRFLPHDESSRNQHTRRAWRFSVLSLTGSIIIGIGIYICAPLLDF
                     LIGQDFAEAEAMIPWLLPFLPLTAISNAPSNGLLGLGKFLPRAAVYGAGALTSLTCYI
                     LFIPRFHEPWKGAVLGTILGELLLVILGWTLLLHYQRQHNRTLVD"
     gene            3583..>3829
                     /locus_tag="PLP95_06675"
     CDS             3583..>3829
                     /locus_tag="PLP95_06675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB45523.1"
                     /translation="MIAALAVTVTIVRSGNDDDGGTTPDRSGPEPTASQVTTVAQLEL
                     DSHADPFGTQITATITPPDGTDQMQIGLDPSVGLGEWR"
BASE COUNT          663 a         1304 c         1160 g          702 t
ORIGIN      
        1 tggctgcgag tgctcacgcc gtggggctcg agactgagcg gccactcttc gaccgtcccc
       61 gcacgccgtc gaaccggcac cactgcgtgc acagcaaatg gggtcggatt cgccacgatg
      121 gcttcgctgg caaccggggc caggcgcgtg agcgcggtgt ggatcaattc ggtcgcctcc
      181 aactcgatcg aggagtagga ctcctgcgcc tcacgatgca cccacccgat ggaggacccc
      241 ggcaagatgt cgtggaactg aagcgtgagc aacgacttcc acaaccgatc gagtgctacc
      301 gccgggtagc cgcggccagc gtcgcccccg tacgctgcga cgctccacac ttcggcctct
      361 cgcagcagcg cctcgcaccg gcggtttccc gctttggtag ctgcctggga ggtgtaggtg
      421 cctcgatgca tttcgagata gagctccccc acccaccgcg gggcgtcgcg gtactcctcg
      481 atcgccgcgt cgagcatcga tgacggtgcg ccgatttcga ccaggggcga tccctcgagg
      541 tcgcggaccc gcaggaactg ctccaacatg tctgcggtgg ggccgccacc tccgtcgccg
      601 tggccgaacg gcatgagcga acgggtggat cgacccttgt cggcgaaggt tctcaccgcg
      661 tgtgcaagct cgtgtcccga gaagttcgcg ttataggtct ccaccggcgg gaagtgggta
      721 aacaccgtcg agccgtcgat gccctcccac cagaacgaat gatgcggaaa gcgattggtc
      781 gtgttccacg acagcttctg ggtgacgaac cgttcgatgc ccacgtgacg caaaatctgc
      841 ggcaggttcg cgttgtaccc gaacacgtcg ggcagccacc cttcggtgca ttcgaccccg
      901 aagtgctcct cgaagaaccg ctgtccatgg gtgaactggc gaatgagcgc ctcaccgccg
      961 ctgaggttgg catcggcctc cacccacatc cttcccaccg gaatccacgt gccctcggcg
     1021 actgcggcgc cgatgtcgcg gaacacggtc gggtagtgct cgcgcatcca gtcgtagtgg
     1081 agcgcctggc tgcacgcgaa ccggtgctgt gggtaccgct cgatcaacgc cagttgattg
     1141 gtgaaggtcc gggcgcactt gcgacgcgtc tcgcgcagcg gccagagcca cgctgtgtcg
     1201 atgtgagcat ggccgatcgc ggtgatccga tgtgcactcg ccgtcgccgg cgccgacagc
     1261 accgcggcca actccgctcg acccgccggc gccgttcggg caatcgacgc tgcgtcggcg
     1321 aggtcgagac ggtccagcat cgtttcgagc gcgcgaagaa tctcgtggcg tcgcgggtcg
     1381 ccccaatcga gctgcttcat caacccgttc aacgcccgca cgtcgtggcg caacgcctcg
     1441 acctgcggat cgacgagcac gagttcggcc cgactcagtc gatacagcga cgccgaccct
     1501 gcggtggcga gatacgacgc cgggttggga accgctaccg agaggtcggg attcgatgca
     1561 gcctcgaggg ccagttccac cgactcccca ccagaggcgg cgtcggtgat cggcaactca
     1621 tgattgagcg ggttgagtcc gcgaagcggc tgccatcctt cggcggttcg tgtccagaac
     1681 aggccctcgc tctggaaccc cggccggtta ctgaacccga ggtcgatcgc cgctcgcacg
     1741 acgccgccgg cccactcgtt cggaaccgcc cccgcgagcg tgaaccaggt cgtgccccat
     1801 ggtttgccga acgacgagcc aacctccctc gccccaaacc tcgccgccat cgcctccgcc
     1861 acgccgaccg gctcgcccgg cacctcccac cgggccacct ccaacgggac gcgagcacag
     1921 atcctggccg gttcgacgcg ttcgagcacc attcggcgca gacgcgcctc gacccgttca
     1981 cgatcgtcgt gcatgtcccc tcccgaatca caccggcttc aactgttgca ttcaacagca
     2041 tcgtcggtac cctgacgaca tcgctcgaca tcgggcgata cacattccca tgagcgacgt
     2101 cgataacgaa gcagaaaatg aagaggcccg tttcgaaggg gtctttcgcc attttcgcaa
     2161 gggtgggctc ggcaacgatg ccgcctgggg ctcgcttcac gacctgacgc aactgttcgt
     2221 gcagaccgcc tcgtttcttc tgctgggacg caaactcggc gtggaagact atggacgcta
     2281 cgtcggggtc tacggcatcg tcggtccgat cggcggtctg acctggtccg gcgttacctt
     2341 ggcggtcctc caacgtcggc tcggggagcg cgactcgatc aacaagaccg cccgcgactt
     2401 tttcctgctt gcgatcgtgc tcggcgtgtt cgccacctcg ctcggcaccc tcatcggcag
     2461 ccaaatcatc agcgggttgg cggtcacgac gattctggcg gtgatgaccg ccgaactggt
     2521 cgcgaacgcg ttcaaccagg tctcgttgtc gatggtgcag gccgaacatg gattcgcatc
     2581 tgcgacgcgc ctgaagatgg tcgtgttgtt catccgcgtg agcgtgctga tcgcacttgt
     2641 cgccgtcgat tcgctcacca tcgggcatct ggccgtcgga ttcaccatcg ggttcttcct
     2701 gtacacggca ctcctggtgc gcacggttct tcccgcgttc ggaattcgcg ttcagctcgc
     2761 ccgacccagc aacggcattc tgcgcgtgac cgggtcgatc tcgctaccca tcgcagcggg
     2821 cgtgctgcag caagacggcg ataaggcggt tttgaacgcc tacaactttc aacacgatgc
     2881 agggctgtac ggcgcggcct ttcgcgtcgt ttcgatggga ttgatgccgt tgcgcgccct
     2941 cgaggctgcg gcgtttcaac gattcctccc ccacgacgaa agcagtcgga accaacacac
     3001 ccgacgcgcc tggcgctttt cggtgctgtc gctgaccgga agcatcatca tcggtatcgg
     3061 catctacatc tgcgcaccgt tgctcgactt cctcatcggt caggatttcg ccgaggccga
     3121 agcgatgatc ccttggttgt taccgttcct gccgctcacc gccatttcca atgccccttc
     3181 gaatgggctc ctcggacttg ggaagttcct tcctcgagca gcggtctacg gcgccggagc
     3241 gctgacgagc ctgacgtgtt acatcctctt cattccacgg ttccatgaac cgtggaaggg
     3301 agcagtgctc ggcacgattc tcggcgaact cctgttggtc atcctcggct ggacgttgct
     3361 gttgcactac caacgtcagc acaaccggac gctcgtcgat tgactcaacc cagcggctga
     3421 ctcaccgagg cgcagccgtc gggtcacgcg ctcagcggcg ctgctcgact gtgaatgcgc
     3481 ccgcgcgtcg actatgaact catggcgcct atgagtgacc acgaatcgga ccacaagcga
     3541 tcgacctccc gacggaactg gctggtagca ggagcggtcg tagtgatcgc tgccctagcg
     3601 gtcaccgtca caatcgtccg cagcggcaac gacgacgacg ggggtaccac gcccgaccgg
     3661 agcgggcccg agcccaccgc atcgcaggtc acgacggtgg cccagctcga gctcgactca
     3721 cacgccgacc cgttcggcac ccagatcacc gccacgatca ccccgccaga tggcaccgat
     3781 cagatgcaga tcggcctcga tccctcggtt ggattggggg aatggcgcg
//
LOCUS       DAOOMA010000208         3829 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_28840_length_3829_cov_3.357181, whole genome shotgun sequence.
ACCESSION   DAOOMA010000208 DAOOMA010000000
VERSION     DAOOMA010000208.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 3829)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3829)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3829
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28840_length_3829_cov_3.357181"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..768
                     /locus_tag="PLP95_06680"
     CDS             <1..768
                     /locus_tag="PLP95_06680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HPB45524.1"
                     /translation="GEVDIARQELNVFRMKLLGATVRPASSGSRTLKNAVNEAMRDWV
                     ATVESSHYCLGSVMGPHPYPWMVREFHRVLGREAAEQFDAAVGGTPDVVVAAVGGGSN
                     AIGLFSGFIDTDATIIGVEPAGGAAVQRGVPGIVHGMHSYLMQDEFGQVEEAHSISAG
                     LDYPGVGPEHAHLAAIGRVEFHPVSDDEVIEAFKTLSRAEGIIPALESAHALAWVMRE
                     AKRVAGSHVIVNLSGRGDKDVDQMMGLVGHEFLGEES"
     gene            765..1586
                     /gene="trpA"
                     /locus_tag="PLP95_06685"
     CDS             765..1586
                     /gene="trpA"
                     /locus_tag="PLP95_06685"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HPB45525.1"
                     /translation="MSVVGAAGVVGVSPEPGPIEARFLANRQAGRKSLVIYLTGGLPG
                     WQDQVRAAAQAGADLIEIGIPFSDPVMDGPVIQESSQRALAHGATPVSVLDEASRLDA
                     GCLLAVMTYANLVYRFGYERFARTAKDSGIDGVILPDIPIEESAPWCAAADSAGIETI
                     MLAAPTASDERLARVAERARGFVYGVGLLGITGERDALSASALEIARRLRGVTDMPVL
                     VGVGISTPAQAAETSKISDGVIVGSAVVRRAIEVGTAESVGELVGEFRSVLDAEC"
     gene            1653..1949
                     /locus_tag="PLP95_06690"
     CDS             1653..1949
                     /locus_tag="PLP95_06690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012229432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HPB45526.1"
                     /translation="MNKTELVETIAQRTDLSKKDVDAVLGAFWDIIAQTVAKGKDTVT
                     IPGWIKFEQVTRDPRVVRNPQTGEQMKIGKTKAVKITAGATLKAIAKGDKPAPK"
     gene            2060..2521
                     /gene="bcp"
                     /locus_tag="PLP95_06695"
     CDS             2060..2521
                     /gene="bcp"
                     /locus_tag="PLP95_06695"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HPB45527.1"
                     /translation="MTTPAEGKKAPAFTLVDQNEAKVQLSKLAGRKVLVYFYPRADTP
                     GCTTQACGLRDIAGQVGDTMIIGISPDKPAKLKKFDDKYELGFTLLSDEDHAIAEKYG
                     VWVEKKNYGKTYMGVQRSAFLIDETGRIEVAWPKISPKDTPTNLLAALAED"
     gene            complement(2531..2665)
                     /locus_tag="PLP95_06700"
     CDS             complement(2531..2665)
                     /locus_tag="PLP95_06700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB45528.1"
                     /translation="MGSTNGSTSSPRRHRRLPLLIAAIAVVAFLRERQFAKNRSALHP
                     "
     gene            2692..2774
                     /locus_tag="PLP95_06705"
     tRNA            2692..2774
                     /locus_tag="PLP95_06705"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2725..2727,aa:Leu,seq:caa)
     gene            complement(2849..>3829)
                     /locus_tag="PLP95_06710"
     CDS             complement(2849..>3829)
                     /locus_tag="PLP95_06710"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HPB45529.1"
                     /translation="GYLREWLSDTLPATVRPAPLFSYEQNLERHVMPRIGDMKLKQLT
                     GPALNRLYGQLLAPGANLRSPKRGLSPRTVQYIHTVLHRALKDAVRHGLLQSNPADLA
                     DPPPSTAVQKAGLKVWTGAELAAFLQQSEAEGDRDHPLWRLIAATGMRRGEALGLRWA
                     DVDLGAGTVTVTRTLIIVDHVAQFGQPKTAAGARTIALDERTTAALRSHRARRAAERL
                     SLGQGQASSDELAFADTSGDWLHPEAVSKRFDRRANRYGLPHIGVHGLRHTWATLALR
                     AGVHPRIVQQRLGHSTIAVTLGVYSHVTDGMDLGAAQQVAALFEPTVTNL"
BASE COUNT          656 a         1156 c         1364 g          653 t
ORIGIN      
        1 ggcgaggtcg atatcgcccg ccaggaactc aacgtctttc ggatgaagtt gctcggtgcc
       61 acggtgcggc cggcaagctc gggaagccgc accctcaaaa atgcggtgaa cgaggcgatg
      121 cgcgactggg tggcgaccgt ggagtcgagc cattactgcc tcggttcggt gatggggccg
      181 cacccgtatc cgtggatggt gcgcgagttt caccgagtgc tcggccgtga ggcggccgag
      241 cagttcgacg ccgcggtcgg tggcaccccg gacgtcgtgg tcgccgcggt gggtggagga
      301 tcgaatgcca tcgggctgtt cagcgggttc atcgacaccg atgccaccat catcggcgtg
      361 gaacccgccg gaggggcggc ggtgcagcgc ggggttccgg gcattgtgca cggcatgcac
      421 agctatctga tgcaggacga gttcgggcag gtcgaggagg cacactccat ttcggcgggg
      481 ctcgactacc cgggggtggg gcccgaacat gcgcatctcg ccgccatcgg ccgcgtcgag
      541 ttccaccccg tcagcgacga cgaggtgatc gaggctttca agaccctgag ccgggccgag
      601 gggatcatcc cggcgctcga gtcggcacac gcgctcgcct gggtcatgcg tgaggcgaag
      661 cgggttgccg gcagccacgt gatcgtgaac ctgtcgggtc gtggcgacaa ggacgtcgat
      721 cagatgatgg gcctcgtcgg ccatgagttc ctgggggagg agtcgtgagc gtcgtgggtg
      781 cagcaggcgt cgtgggagtt tcgccggagc cggggcccat cgaggcgcga ttcctcgcca
      841 accgccaggc tggccgaaag agcctggtga tctacctcac tggcgggctc cccggctggc
      901 aggaccaggt acgcgccgca gcgcaggccg gtgccgacct catcgagatc ggtatcccct
      961 tttccgaccc ggtgatggac gggccggtga tccaggagag ttcccagcgc gccctcgcgc
     1021 atggggccac cccggtgagc gtgctcgacg aggcgtctcg tctcgatgcc ggatgtctgc
     1081 tggcggtcat gacctacgcg aatctcgtct accgattcgg ttatgagcgt ttcgcccgca
     1141 ccgcgaagga ttccggcatc gacggtgtca ttttgccgga catcccgatc gaggagtctg
     1201 ctccgtggtg tgcggccgcc gattccgctg gcatcgagac gatcatgttg gccgcgccga
     1261 cggcctccga tgagcgtctc gcccgcgtcg ccgaacgcgc tcgggggttc gtgtatggcg
     1321 tcgggttgct cggcatcacc ggtgaacgag atgcgctttc ggcctccgca ctcgagatcg
     1381 ctcgacgcct gcgcggggtg accgacatgc cggtgttggt cggtgtcggc atctcgacac
     1441 cggcccaggc ggcggagacc tcgaagattt ccgacggggt gatcgtcggc agcgcggtcg
     1501 tgcggcgcgc catcgaggtg ggtaccgccg aatcggtcgg tgagctcgtc ggggaatttc
     1561 ggagcgtttt ggacgccgaa tgctgacaaa cccccgaaaa ctcggtaaag tcacaccgtt
     1621 gtaacttcaa tgcacccggg agggtcagga acatgaacaa gactgaactg gtcgagacca
     1681 tcgctcagcg caccgatctg tcgaagaagg acgtcgacgc agttctcggc gcgttctggg
     1741 acatcatcgc tcagaccgtc gccaagggta aggacaccgt caccatcccc ggctggatca
     1801 agttcgagca ggtcacccgt gacccccgcg tcgtgcgcaa cccgcagacc ggcgagcaga
     1861 tgaagatcgg caagaccaag gctgtgaaga tcaccgccgg cgccacgctg aaggccatcg
     1921 ccaagggcga caagcctgct ccgaagtgag ccgcagcggc gccgtaggtc ggcgccgctc
     1981 agcaagaaaa tctcgttgag gggagggcgc gaccgcgccc ttccctcaac gcgttgggcg
     2041 cggtgtctag attgggccca tgacgactcc cgctgaaggc aagaaggccc ccgcgttcac
     2101 cctggttgac cagaacgagg ccaaggtgca gctgtccaag ctggccggcc gcaaggtcct
     2161 ggtgtacttc tacccgcgag ccgacacccc cggctgtacg acccaggcgt gtgggctccg
     2221 cgacatcgcc gggcaggtgg gagacacgat gatcatcggc atcagccccg acaagccggc
     2281 caaactgaag aagttcgacg acaagtacga actcggcttc acgctgctgt ccgacgagga
     2341 ccacgccatt gccgagaagt acggggtgtg ggtcgaaaag aagaactacg gcaagaccta
     2401 catgggggtg cagcgcagcg ccttcctgat cgacgagacg ggacgcatcg aagtggcctg
     2461 gccgaagatc agcccgaagg acacaccgac caacctgttg gcggcgctcg ccgaggactg
     2521 aacgccttcg ctacgggtgc agcgccgagc ggtttttggc gaattgccgt tctcggagaa
     2581 aggcgacgac cgcgatggcg gcgatgagca acggcagccg tcggtggcgc cgtggcgaac
     2641 tcgttgaacc gttcgtggaa cccatggcga ccacgctaga gtgtgcgccg ggcccgggtg
     2701 gcggaatggc agacgcggtg gactcaaaat ccactgtccg aaagggcgtg tgggttcgaa
     2761 tcccaccccg ggcacgcttg tgatggagtg atcaccccga gaacattggg aacgcggtgt
     2821 tcggttcagt gccgtcgacg gcctgtggtc acagattggt cacagttggc tcgaagagcg
     2881 ctgcgacttg ctgtgctgcg ccgagatcca tgccatcggt gacgtggctg tacacgccga
     2941 gtgtgaccgc gatggtgctg tgtcccaggc gctgctgaac gattcggggg tggacccctg
     3001 ctcggagtgc gagcgtcgcc caggtgtgcc gaaggccgtg gacgccgatg tgggggagtc
     3061 cgtagcggtt cgcccgtcgg tcgaaccgct tggaaactgc ttcggggtgc agccagtcgc
     3121 cgctggtatc tgcgaacgcc aattcgtccg acgaggcctg accctgaccg agggacaggc
     3181 gctctgcggc ccgacgggcg cgatgcgatc ggagcgcggc cgtcgttcgt tcgtcgaggg
     3241 cgatggttcg ggctcccgct gccgtcttgg gttgtccgaa ctgagcgacg tggtcgacga
     3301 tgatcaacgt cctggtgacc gtgacggttc cggcgccgag gtcaacgtca gcccagcgga
     3361 gaccgagcgc ttcgccgcgg cgcatgcccg tggcggcgat gagtcgccag agtgggtggt
     3421 ctctgtcgcc ctccgcctcc gactgttgga ggaaggccgc gagttcggcg ccggtccata
     3481 ccttgagccc agccttctgg acggcggtcg acggaggtgg gtcggcgagg tctgccgggt
     3541 tcgactggag gaggccgtgc cgaacggcat ccttcaatgc ccgatggagg acggtgtgga
     3601 tgtactggac tgtgcgaggt gaaaggccgc gtttggggga gcgaaggttg gcgccgggcg
     3661 caaggagctg tccgtagagc cgattgagcg ccggtccggt gagctgcttg agcttcatgt
     3721 cgccgatgcg cggcatgacg tgacgctcga ggttctgttc atagctgaac agcggcgccg
     3781 gccggacggt ggccggcagc gtgtcggaca gccactctcg gaggtagcc
//
LOCUS       DAOOMA010000209         3828 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_28858_length_3828_cov_3.508614, whole genome shotgun sequence.
ACCESSION   DAOOMA010000209 DAOOMA010000000
VERSION     DAOOMA010000209.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 3828)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3828)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3828
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28858_length_3828_cov_3.508614"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..533)
                     /locus_tag="PLP95_06715"
     CDS             complement(<1..533)
                     /locus_tag="PLP95_06715"
                     /inference="COORDINATES: protein motif:HMM:NF025495.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dsDNA nuclease domain-containing protein"
                     /protein_id="HPB45530.1"
                     /translation="MAGGLEDALATVGPGDTSGSETFARYLYQCKVAVQRWLATIEAA
                     EDAFILCEFVDDVTTVTSAEICFAQVKTRDRGAWTAAKVLTAGGGIDALVRSFGLAKA
                     VENVPPIRLELNLEGPEGPRADTRTFFADPTAASVAQRSAIVDLGLSEVDVDEFLACL
                     KVVRQYHARPSIDAVTIR"
     gene            complement(1299..1375)
                     /locus_tag="PLP95_06720"
     tRNA            complement(1299..1375)
                     /locus_tag="PLP95_06720"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1339..1341),aa:Met,seq:cat)
     gene            1842..2702
                     /locus_tag="PLP95_06725"
     CDS             1842..2702
                     /locus_tag="PLP95_06725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB45531.1"
                     /translation="MRGIIHLGSLPTPLPDDSVWQAWSWEDVLGRHATSDNTWVSLTA
                     RAWIEHIDTALPMADGDTTWSGIALEDSPVAALRVRSAWLVSQLSAEAHAKGIRIAST
                     SGRSGKRWGLEMRRPTSLPDYDIVVYLHERTRSDEIPSTGGTLFIPRGPEALVSLRHR
                     GAATSDDFDWNHLLVLSSTFASARDDWFHTRKPNLVGVDRDRYSDFVAAGGPPHLGFG
                     VGDEPTFHTDDIAFGAKITFRPDVTLSTLSRELSALMDTVVALSEVPPSTTPSVGTVV
                     SPNPLPPIID"
     gene            2754..3560
                     /locus_tag="PLP95_06730"
     CDS             2754..3560
                     /locus_tag="PLP95_06730"
                     /inference="COORDINATES: protein motif:HMM:NF022114.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gmad2 immunoglobulin-like domain-containing
                     protein"
                     /protein_id="HPB45532.1"
                     /translation="MTDLRDINNSCASHVPTPDSFTRSVSTHRRATSRRIASVVLASA
                     LCLGVVACGNADDDAAVDSDATTTAASTDSAISPTTTTGSSGEEPDTQDVVAVWPLLN
                     GDTFDDPVAAATSFAVDLARFTDPIVGEFMQGDNRSGEVEVRATDNGPTTTVMVRLDA
                     DDHWVVIGAATSNIVPDQPTGAVTSPVTVSGTSTAFEATVDVAVYDRDSTAPLVRTFV
                     MGGSMGDMGPFSESIEFEDPTTPTGTILYWTTSAKDGSVSEAAAVTVSFG"
     gene            complement(3549..>3828)
                     /locus_tag="PLP95_06735"
     CDS             complement(3549..>3828)
                     /locus_tag="PLP95_06735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB45533.1"
                     /translation="GANGVNLYRANMMTKMRRPRRVHTDIAVQVVVPLRDRYVSPALA
                     ESVVGLCDDLVFRQVDAGHWVVLSDPDSIAEWIAEHVDRVDAGRSSAE"
BASE COUNT          669 a         1369 c         1146 g          644 t
ORIGIN      
        1 cgtatcgtga cggcgtcaat cgacggacga gcgtggtact gccggacaac cttcaaacac
       61 gcgaggaact catcaacatc gacctcggac agacccaagt cgacgatcgc cgacctttgg
      121 gcgactgacg ctgcggtcgg gtcggcgaag aaggtcctcg tgtcagcgcg cggcccctcg
      181 ggcccttcca agttcagctc cagccggatc ggcggcacat tctccactgc cttcgcgagg
      241 ccgaacgaac gaacgagcgc atcgatgccc ccgcctgcgg tgaggacctt cgccgccgtc
      301 catgcacctc ggtcgcgcgt cttgacttga gcgaagcaga tctccgcgct cgtgacagtt
      361 gttacgtcat cgacgaactc gcagaggatg aaggcgtcct cggccgcctc aatcgtcgcc
      421 aaccatcgct gaaccgccac cttgcactga tacaggtacc gagcgaacgt ctcgctgcca
      481 ctggtgtcgc ccggtccaac agtcgctagc gcatcttcaa gaccgccggc catcagccgg
      541 cccgagacgc tgtcggaagt tggtcctcat gtcccgcgat cgcgaaggca tcggcgaatg
      601 agatgccgac ccgctggcgt gctggtggcg tggtacctgc ttcgagtgat gcgaccatgg
      661 gagaccgtca ggtccccggc cccctcggct cgcgtcaacc cgacaagctg agaccgtacg
      721 ccactcggct cctactgttc gtcgaatggc gactcacctg atcgccccgg ccagcgggtg
      781 gcgtgcgatc gagaacctgg ttccaccact accggccacg acaatggaac cctcgctgcg
      841 gcgagcaggg ccggcggcgg cagaggttgg aacggctggc agccagcccg ggtctccagg
      901 ttcatcgaga ttccgctacg aggccccggg agaacggacc tcgacctatg tagtgcgcgc
      961 tctgttggcc cccgccgggt gatggggttg cctgagagtt cgacaaccgc ggtgcgcctc
     1021 tcagaatgac gtggcagggg gcaccctgcg aggcgccacg cgatctcacc cgccgcggcg
     1081 ggtgaggcgc cgtccgccgg gagcctgccc gccagaaccc gaaggtcaca tcggagcgcc
     1141 cgcgtggacg catctcgctt gctcccgacc gatcgcgtga cgaaccgtcg tcggctgtcc
     1201 aaggcgaccc ggtagaaaac ccggtagaag aatggccgaa aacgtaggaa cggccctctc
     1261 cgaatctctc ggaaagggcc gttgacctgg tgtttcgttg gtggcggggg caggatttga
     1321 acctgcgacc ttcgggttat gagcccgacg agctaccgaa ctgctccacc ccgcgatgag
     1381 gtgacgtcac cctagcaacc ccaaccgcgt ccgacaaaac gaccacttgg gcaggctctc
     1441 aggccgccca ccatccccga aaccgccaaa gttgcggtat tcgacctccg cttcggccgc
     1501 atatcggcta cacaaagggc acgcggttcg ttcgaaaggt gtccggtgac ctccccgctt
     1561 ccttctctca cctacctgct cgccctcagt gacgaatcga gttgggccga cttgttggcc
     1621 actttgatcc tcaccgaccc ggacaccctc gccggcctgc tcggcggagt cgactcgaac
     1681 gcattcctgg ttcgccggga ctttccactc ggccgcggcc gcaccgtgga tctcgtggtg
     1741 accgccgccg gtcggccgat cgtggcgctt gagacctcgg tgatggagga catcaacccc
     1801 cacctgctcg ccgcgatcga tgccgacttc ccggggaatg cgtgcgcggc atcatccatc
     1861 tcggctcgct tccgacgccg cttcccgacg actccgtgtg gcaggcctgg agttgggagg
     1921 atgtcttggg gcgtcacgcg acgagcgaca acacgtgggt gtcgctgacg gcccgagcct
     1981 ggatcgaaca catcgacacc gcgctgccga tggccgacgg cgacacgacg tggagcggca
     2041 tcgccctcga ggacagcccc gtcgcggcat tgcgcgttcg ttccgcatgg ctcgtgtcac
     2101 agctcagcgc cgaagcccac gcgaagggaa tccgtatcgc ctccacctcg ggtcgcagcg
     2161 gcaagcgttg gggactcgag atgcgtcgac cgacttctct gcccgactac gacatcgtcg
     2221 tctacctgca cgaacgcacg cggtctgacg agattccttc caccggcggg acgctgttca
     2281 tcccccgggg ccccgaggcg ctcgtctcgc ttcgccatcg cggcgccgcg acctccgacg
     2341 acttcgactg gaaccacctc ctggtgctct cttcgacgtt cgcctcggcg cgcgacgatt
     2401 ggttccacac ccgcaagccc aacctggtcg gcgtcgaccg cgaccgctac tccgacttcg
     2461 tcgccgccgg cggcccgcca cacctcgggt tcggcgtggg cgatgaaccg acattccaca
     2521 ccgatgacat cgccttcggc gccaagatca ccttccgtcc cgacgtcacg ctgtcgacgc
     2581 tcagccgcga actctccgcg ctgatggaca ccgtcgtggc gctgtcggag gtgccaccgt
     2641 cgacaacccc gagcgttggg actgtcgtgt cgccgaaccc gcttcccccg atcatcgact
     2701 agccgaccca ccctcaccat ccaccctcac cgacgattcg aatcgagacc gacatgaccg
     2761 acctccgcga catcaacaac tcctgcgcat ctcacgtgcc aacaccagat tccttcactc
     2821 gatcggtatc cacgcaccga cgcgccacct cacgccgtat cgcttcggtc gtgttggcct
     2881 cggcgctgtg cctcggagtg gtcgcgtgcg gcaatgccga cgacgacgcc gcagtcgact
     2941 ccgacgctac gacgactgcc gccagcaccg actcggccat ctcaccgacg accacgaccg
     3001 gttcgtcagg cgaggaaccc gacacccagg acgtcgtcgc agtgtggccg ctcctcaacg
     3061 gcgacacctt tgacgatccg gtcgccgcag cgacgtcgtt cgccgtcgat ctggccaggt
     3121 tcaccgatcc gatcgtcggc gagttcatgc agggcgacaa ccgctccgga gaggtcgagg
     3181 tgcgcgccac cgacaacggc ccgaccacca cggtgatggt tcgcctcgac gcggacgacc
     3241 attgggttgt catcggcgcg gctacgtcga acatcgtccc cgatcaaccg accggagccg
     3301 tgacctcccc cgtcaccgtg agcggtacga gcaccgcctt cgaagcgacc gtcgatgtcg
     3361 ccgtgtacga ccgcgacagc accgctccgt tggttcgcac cttcgtcatg ggcggctcga
     3421 tgggggacat gggcccgttt agcgaaagca tcgaattcga ggatccgacg acgccgaccg
     3481 gcaccatcct ctattggacg acctcggcta aagacggaag tgtcagcgag gcagccgcgg
     3541 tgaccgtctc attcggctga tgaccggccg gcatcgacgc ggtcgacgtg ttcggcgatc
     3601 cactcggcga tcgaatcggg gtcgctgagc accacccagt ggccggcatc gacctggcga
     3661 aacaccaggt cgtcgcataa tccgacgacc gactcggcca acgccgggct cacatagcga
     3721 tctcgcaacg gaaccaccac ctgcacggcg atgtcggtgt gcacgcgccg cggtcggcgc
     3781 atcttggtca tcatgttggc ccggtacagg ttgacgccgt tcgccccg
//
LOCUS       DAOOMA010000210         3800 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_29144_length_3800_cov_3.358879, whole genome shotgun sequence.
ACCESSION   DAOOMA010000210 DAOOMA010000000
VERSION     DAOOMA010000210.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 3800)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3800)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3800
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29144_length_3800_cov_3.358879"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..370)
                     /locus_tag="PLP95_06740"
     CDS             complement(<1..370)
                     /locus_tag="PLP95_06740"
                     /inference="COORDINATES: protein motif:HMM:NF024966.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HPB45534.1"
                     /translation="MSSRTFADVSEGVRATIARYTLALDDGRTDDVIATFAADGSFEL
                     EGKPPAAGIEALRTMYERWVPRVPQRHLVSNTLVDTWSDTAATAISDVVLLVKIDEAW
                     SVQFVARYHDLLVADGDTWKF"
     gene            489..1844
                     /locus_tag="PLP95_06745"
     CDS             489..1844
                     /locus_tag="PLP95_06745"
                     /inference="COORDINATES: protein motif:HMM:NF013044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HPB45535.1"
                     /translation="MTHAWSKQPIPYAVEVPDRAPKERYYSQEFFDLEAEQLWSRTWQ
                     MACRLEEIPEPGDFTIYEILDQSVIVIRGEDGEIRAMHNTCRHRGVKLATAPGNCAKE
                     FVCPFHGWCYDQSGRNTKIPMRKSFAQHNIDADDVSLAPVQCDTWGGCVWINLDPEAP
                     PLRESIEPAASILDAWKVEDLRVEWWYACRLPVNWKLAQQAFQEMYHVVVTHPQLVIA
                     GMRYGAQHGEFDPKRFVDAEIQYLHTMSEGMAGMVHATDVAVAESIRDIDLPADPAAA
                     MAAWYLALNTAVVEHHRSLGHEVPDLVDLEAKGLAEPMGYVFPHYFVLPMYSSASSYR
                     FRPVGPEETIMEIWSMTRYLSDAERPRPVPPEIWPHDDPRWPPIPAQDFSNLPRQQLG
                     LHSKAFEFMRLSQTGEGHLSNFERVLDGFLAGLPHDRLASALREVNVNPLERHVVDIE
                     F"
     gene            complement(1980..2360)
                     /locus_tag="PLP95_06750"
     CDS             complement(1980..2360)
                     /locus_tag="PLP95_06750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB45536.1"
                     /translation="MTAAQVRAYAAKSQEFAEAAASDLDAGRNIAATSLAIHAAINAA
                     DAVCGARLGKRSGGDDHDQALSLLQVAGTDGAEVERHLRRLLPLKTKAEYDPDDIAAS
                     VAKRAVERALQCAAIARRVAVSVR"
     gene            complement(2380..3003)
                     /locus_tag="PLP95_06755"
     CDS             complement(2380..3003)
                     /locus_tag="PLP95_06755"
                     /inference="COORDINATES: protein motif:HMM:NF037100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HPB45537.1"
                     /translation="MDFVRPIEAIVPGAQGRVLEVLARTTDELNLRTIARLAGISPAQ
                     TSRVLPRLAYLGIVRRREVPPASLFSLALDHVAAGPLLALAASRQSVLDEIGRAASEL
                     VDLPISAIVFGSLARGEADAESDIDLAVVRPTHIDEDDDSWVDGLESLRQRVRDVSGN
                     QVEILEVGVDDVAHKLTSDNPVWADICREGRVVYGRSIDELREIKVG"
     gene            complement(3190..>3800)
                     /locus_tag="PLP95_06760"
     CDS             complement(3190..>3800)
                     /locus_tag="PLP95_06760"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HPB45538.1"
                     /translation="LVASGATAISVAVDVADHDSVVAAFGQVRNELGNTEILVTSAGI
                     EGFDSLLDVSPQRWEQIIAVNLTGTFWCVQEAVPQMIDAGFRRIVTISSSSALSGARR
                     MTHYVASKGGVIGLTKALAAELAPQRITANSIAPCLIDTPMVDQAVESGDFPGVEAVA
                     PHIPLGRAGTPDDTAAAAAYLASDGAEYITGQTLTVAGGMYL"
BASE COUNT          671 a         1218 c         1249 g          662 t
ORIGIN      
        1 cgaacttcca ggtgtcgccg tctgcgacga ggagatcgtg atagcgtgcc acgaactgca
       61 ccgaccacgc ctcgtcgatc ttgaccagca acacgacgtc gctgatcgcg gtggccgcgg
      121 tgtcactcca tgtgtcgacg agcgtgttgg acacgagatg gcgttgtggg actcggggca
      181 cccagcgctc gtacatggtg cgcaacgcct cgattcccgc cgccgggggt ttgccctcga
      241 gctcgaagga cccgtcggcg gcgaacgtcg cgatgacatc gtcggtccga ccatcgtcga
      301 gggccagcgt gtagcgggcg atggtcgccc ggaccccttc ggacacgtct gcgaatgtgc
      361 gactcgacat caaatccccc tgatcgacgg tgtttatcgg aaccgtcgtt gaatgtaccg
      421 accggttggt ctaaacctac tgcaacgatg tcgccgggga cgtcccgagc ggcctcaggg
      481 ggatggtcgt gactcacgcg tggtcgaaac agccgattcc gtatgcggtc gaggtgccgg
      541 atcgggcgcc gaaggagcgc tactacagcc aggagttctt cgatctcgag gccgagcagc
      601 tgtggtcgcg gacctggcag atggcctgtc gtctcgaaga gatccccgaa ccgggagact
      661 tcacgatcta cgagattctc gaccagtcgg tgatcgtcat ccgcggcgag gacggcgaga
      721 tccgggcgat gcacaacacg tgtcgtcacc gtggggtgaa gctggcgacc gcgccgggca
      781 actgcgccaa ggaattcgtc tgcccgtttc atggctggtg ttacgaccaa agcgggcgca
      841 acacgaagat cccgatgcgt aagtcgtttg cgcagcacaa catcgacgcg gatgacgtga
      901 gcctcgcccc ggttcagtgc gatacgtggg gtgggtgtgt gtggatcaac ctcgaccccg
      961 aggcgccgcc gctgcgtgag tcgatcgagc cggcggcgag catcctcgat gcgtggaagg
     1021 tcgaggatct gcgggtcgag tggtggtacg cgtgccgcct tccggtgaac tggaagctgg
     1081 cccagcaggc gtttcaggag atgtaccacg tcgtggtcac ccaccctcag ctcgtcatcg
     1141 cggggatgcg atacggcgcg caacacggcg agttcgaccc caaacgcttc gtcgacgccg
     1201 agatccagta cctgcacacg atgagcgagg gcatggcggg gatggtgcac gccaccgacg
     1261 tcgcggttgc ggagtcgatc cgcgacatcg acctgcccgc cgacccggcc gcggccatgg
     1321 cggcgtggta cctggcgctc aacaccgctg tggtcgagca tcatcgcagc ctcggccacg
     1381 aggttcccga cctcgtcgac ctcgaggcca aggggctcgc cgagccgatg ggctacgtgt
     1441 tcccgcacta cttcgtgttg ccgatgtact cgagcgcgtc ctcctatcgc ttccgcccgg
     1501 tcggacccga agagacgatc atggagatct ggtcgatgac ccggtatctc tccgacgccg
     1561 aacggcctcg cccggtcccg ccggagatct ggccacacga cgatccgcgt tggcccccga
     1621 ttccggcgca ggacttctcg aacctgccgc gtcaacaact cgggctgcac tcgaaggcct
     1681 tcgagttcat gcgactgtcg cagacggggg agggccacct gtcgaacttc gaacgggtcc
     1741 tcgacgggtt cctcgccggg ttgccccacg accggctcgc ttcggcgctt cgtgaggtga
     1801 acgtcaaccc gctcgaacgc cacgtcgtcg atatcgagtt ctgatcgacg cgccgccggc
     1861 gcgcgggtgc cggcgctggc gcgcgaactt tgtgcggaaa gtgacaccac gtgtcgcttt
     1921 ccgcacaaag ttcgcgggat cccgacaggt gcttcgcgaa agcctgtcag cgtcgacgat
     1981 cagcgcacgg aaacggcgac ccgtcgagcg atagcggcgc actgaagtgc tcgctcgacc
     2041 gctcgcttgg ccaccgacgc agcgatgtca tcggggtcgt actctgcctt tgttttcaag
     2101 ggaaggaggc gccgcaggtg acgctcgacc tcggccccgt ccgttcctgc aacctgcagg
     2161 aggctcagag cctggtcgtg gtcgtcgccg cccgaccttt tgcccaggcg cgcaccgcaa
     2221 actgcatcgg ctgcgttgat cgccgcgtgg atggcaaggc tcgtggctgc gatgttgcgg
     2281 ccagcatcga ggtcgctcgc ggccgcttcg gcaaactcct gcgacttggc cgcataggcg
     2341 cgtacctgcg ctgcggtcac aggccgagtc cgcccctgct tacccaacct tgatctcccg
     2401 aagttcgtcg atggaacggc cgtagacgac tcgcccctcg cgacagatgt ccgcccagac
     2461 cggattgtcc gaggtcagct tgtgcgccac gtcatcaacg ccgacttcga ggatctcgac
     2521 ctgatttccg ctgacgtcgc gcacccgctg ccgcaaggat tcgagcccgt cgacccagga
     2581 atcgtcgtcc tcgtcaatgt gggtgggacg tacgacggcg aggtcgatat cgctttcggc
     2641 gtcggcctca cctcgcgcaa gcgagccaaa cacgatggcg ctgatcggta ggtccacaag
     2701 ttcacttgct gcgcgtccga tctcgtcgag gaccgactgc cgcgaagccg cgagggcaag
     2761 caaggggcca gcggccacgt ggtcgagagc aaggctgaac aacgatgctg gtggcacctc
     2821 ccgccggcga acgatgccga ggtaggcgag tcgtggaagc acccgcgagg tttgtgcggg
     2881 gctgatgccg gccaagcggg caatcgtgcg aaggttcagt tcatcggtcg ttcgcgcgag
     2941 tacctcgagc acccgtccct gagccccagg cacgatcgcc tcgatcggtc gcacgaagtc
     3001 catgtgcaga gtctatccgg ataactgcat ttatggaacg agtgtttcag aaaagaggtg
     3061 aatagctgcg acggcgtgtc cgaacgcgaa ctttgtgcag aaagtgacac cacgtgtcga
     3121 cttccgcaca aagttcgcgg gatcccgccg aagagagccc gccgagcccg gccgacgatc
     3181 agccgatgat cagaggtaca tgccgccggc gaccgtgagg gtctgcccgg tgatgtactc
     3241 ggcgccgtcg gacgcgaggt aggccgccgc tgcggcggtg tcgtcgggcg tgccggcgcg
     3301 ccccaacggg atgtgcgggg caacggcctc tacccccggg aagtcgccgg actcgaccgc
     3361 ctggtcgacc atcggcgtgt cgatgagaca cggagcgatc gaattggcgg tgatgcgctg
     3421 cggtgcgagt tcggctgcga gcgctttggt gagcccgatg acgccaccct tggaagcgac
     3481 atagtgggtc atgcgccgcg cccccgacag tgcgctcgac gacgagatgg tgacgatgcg
     3541 gcggaagccg gcgtcgatca tctgtggaac cgcctcctgg acgcaccaga aggtgccggt
     3601 caggttcacc gcgatgatct gttcccaacg ctgcggggag acatcgagca gcgaatcaaa
     3661 gccctcgatt cccgcgctcg tgacgagaat ctcggtgttg ccgagttcgt tgcgaacctg
     3721 cccgaacgca gccaccaccg agtcgtggtc ggcgacatcc accgccactg agatggctgt
     3781 cgcaccggaa gcgaccaact
//
LOCUS       DAOOMA010000211         3794 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_29189_length_3794_cov_3.829366, whole genome shotgun sequence.
ACCESSION   DAOOMA010000211 DAOOMA010000000
VERSION     DAOOMA010000211.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 3794)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3794)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3794
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29189_length_3794_cov_3.829366"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..740)
                     /locus_tag="PLP95_06765"
     CDS             complement(<1..740)
                     /locus_tag="PLP95_06765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB45539.1"
                     /translation="MNDLHGTMNSNEALAAFGDVVDRVSKGHETGSGDPGIQTSALSV
                     SGSRVVWPSSFDVTGLAVGAVANATLAAARLWELRNDLGTTPRVFVDSRAACAAFALE
                     SRFEPIGWERPPIWDPIAGNYQTANGWIRLHTNYASHRSAVEEVLGAHDRAGVQAAVA
                     AMDSNELEDAVVDNGGAAAAMRTREQWLGSEAGSATAAPTALATTWARAVAAPSLRRL
                     RQYASTSRSSVARPATSSLPNRASSTIGA"
     gene            1470..2057
                     /locus_tag="PLP95_06770"
     CDS             1470..2057
                     /locus_tag="PLP95_06770"
                     /inference="COORDINATES: protein motif:HMM:NF012506.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046912 - acyltransferase activity, acyl
                     groups converted into alkyl on transfer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/2-methylcitrate synthase"
                     /protein_id="HPB45540.1"
                     /translation="MASVVAAQGDPCPGPGDEHRSRPARGTRPRPSALAVRVAASARV
                     RPDTCLIAGLAALDGALHGRASAAVHREFLESGRSRADEPSPSLGVGHVVHRNDPRTA
                     MLLDAVSAIATAGDRQLIEAVATEAGGSANIDLALAALAYVGRMPVGASTAVFAIART
                     AGWIAHAQEEYDEQPLRFRGQAIHRTANASTQRPG"
     gene            2171..2243
                     /locus_tag="PLP95_06775"
     tRNA            2171..2243
                     /locus_tag="PLP95_06775"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2204..2206,aa:Lys,seq:ctt)
     gene            complement(2555..2980)
                     /locus_tag="PLP95_06780"
     CDS             complement(2555..2980)
                     /locus_tag="PLP95_06780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB45541.1"
                     /translation="MGTFIATWNPKKSELSPQDLAAAITDTSAGKVWRGAWSMGARTQ
                     GIVPGDRVFLLRQNVDRGLIASGVAASDIYLAEKWDGSGADAPYVDVEWDRVLDPADR
                     LPTELLQGLVPGVPWRFLRASGGEEKEGLGAQEDRETGM"
     gene            complement(3047..3724)
                     /locus_tag="PLP95_06785"
     CDS             complement(3047..3724)
                     /locus_tag="PLP95_06785"
                     /inference="COORDINATES: protein motif:HMM:NF016041.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HPB45542.1"
                     /translation="MAALVVAALAIVGCFAAGAFAWTLGEYLLHSSFHWAKGKGLASR
                     EHLGHHVRASWRFDPVLLLAWLGVVLVGAGLAWLGSRWVPSVVAFSFSAGWTVGYFFY
                     EWHHRAAHLRGPRNRWERWLRINHFQHHFGHPTKNQQVTIPLWDHVFGTAYTADVVRV
                     PRRLAMPWLLDEDGNLRPEFVGDYVIVGDDRSTTERGIALDTARAFANLAPTSNDPAS
                     EVGARVR"
BASE COUNT          672 a         1334 c         1174 g          614 t
ORIGIN      
        1 gcgccgatgg tcgaactggc gcgattgggc aagcttgatg tcgccgggcg tgcgacgctc
       61 gagcgactgg tgctcgcata ctggcgcaag cgcctcaagc tcggggccgc aacggcccgg
      121 gcccaggtgg tcgccagtgc ggtcggcgcg gccgtggccg aaccggcttc ggagcccagc
      181 cactgttcgc gtgttcgcat cgccgcagcc gctcccccgt tgtcgaccac ggcatcttcg
      241 agttcgttcg agtccatcgc ggcgaccgcg gcctgaaccc cggcgcgatc gtgtgccccg
      301 agaacctcct caaccgccga tcggtgcgac gcgtagttcg tgtgcagccg gatccatccg
      361 ttcgcggtct gatagttgcc ggcgatcgga tcccagatcg gcggccgctc ccagccgatg
      421 ggctcgaaac gcgactcgag tgcgaaggcc gcacacgcag cccgactgtc gacgaacacc
      481 cgcggtgtcg tgcccaggtc gtttcgaagt tcccaaagcc gtgccgccgc cagcgtcgcg
      541 ttcgccacgg cgcccacggc gagcccagtg acgtcgaacg acgacggcca caccacgcgg
      601 gacccgctca ccgaaagcgc cgaggtttgg atgccggggt cgccggagcc ggtctcgtgc
      661 cccttgctca cgcggtcgac gacatcgccg aaggccgcca aagcctcgtt ggagttcatg
      721 gttccgtgca gatcgttcat ggcacgatcc tccgcccggc acatcccctc atcaagattg
      781 atttacgtca atgttgttga tgagaatgtc cagatgggag agctggtccc ggcccgcgaa
      841 gccgcgctac gacttcgtgt caacgtcaac accctctacg cctacgtcag ccgtggcgct
      901 ctgcgtcggg ttcctggccc cgacggcaag acgagcctgt acgacgccga cgagatcgat
      961 gcgctggcaa gccggtcacg cccgcggacc aaacgtcggg cacccgccag catcgacctc
     1021 ctggtcgcca ccgaaatcag caccgtgacc gacggcgtcg tgcggtaccg agggatggac
     1081 ctgtcggaac tcatcgacga tcacctcccg ttcgagacgg tcgcggagct gctgtggcag
     1141 acgccggcca cacaggtcgg caaaggatgg acgaccgagt gtgccagccg cgggcgtcgc
     1201 cgctcgcccg cgccccacat cgatgagtcg acgaccatcc cccggctcat tgaggtcatt
     1261 acccgacgac aactcgattc cgaccgtgcg ccgctatcga actttggtga ccgtgacgag
     1321 ggcgtcgact ggaccgatgc gggacggcga gcgatagaag caatgctcac ggtcgagggc
     1381 gtcaccagcg actccggttc cggaagcgcc agttccgtga acgccggttc cgggagggtc
     1441 tcgacacgat tcggcgtggc tacacgactg tggcatctgt ggtcgccgct caaggcgacc
     1501 cctgcccggg tccgggcgat gaacaccgct ctcgtcctgc tcgcggaaca cggcctcgcc
     1561 cgtcagcgct ggcggttcga gtcgccgcct cggcgcgagt acgccccgac acctgtctca
     1621 tcgccggcct ggccgcgctc gacggggcgc tccatgggcg cgccagcgcc gcggtgcatc
     1681 gagagtttct cgagtcgggg aggtctcgcg ccgacgaacc ttcaccgtcg ctcggtgtcg
     1741 gccacgtcgt ccaccgcaac gatccccgta ccgcaatgtt gctcgacgcg gtctcggcca
     1801 tcgcaacggc gggcgatcgt caactcatcg aggccgtcgc caccgaggcg ggtgggagcg
     1861 ccaacatcga tctggcgctc gccgccctcg cctacgtcgg tcgaatgccc gtcggtgcgt
     1921 cgacggcggt gttcgccatt gcccgaacgg ccggttggat tgcccacgca caggaggagt
     1981 acgacgaaca accgctgcgt ttccgtggcc aggccatcca ccgcaccgcg aacgcctcga
     2041 cacaacgacc cggttgaacg gacgcccgac ttgggcccca tttgcatttg gcgaccccaa
     2101 cggctgaggc agtaacatga ccaacgccct tgacgggtcg gcctccacga agccgccccc
     2161 gatggcagat gcgcctctag ctcaattggc agagcaacgg actcttaatc cgcaggttct
     2221 gggttcaagt cccagggggc gtacagcgca aacccgccag aaatggcggg tttgggcgtt
     2281 ttcggagaag cgtgtcgacg ctgatcctca agttcacctc ggcaggccgg tcgccaggct
     2341 caaggcgccg ccaagatggc cttcaactct tcgaccgaca acgcaccttg ccgatttcgg
     2401 tgaaccatcc ggtggtagtt cgaacacagg agcgccagat cggacgcttt cgtcgtgccg
     2461 gcgccgaact gggccagcgg gcgagtgtgg tgacactcga tgaagttctc cccccagttg
     2521 cccgtaggtc gccgcgaagt cgaatccaca tgcctcacat gccagtttcc cggtcttctt
     2581 gcgcaccgag tccttctttt tcttcgccac ccgacgctcg gagaaatctc cacgggacac
     2641 cgggaaccag accctgaagc aactcggtcg gcaaccggtc cgccgggtcg agcacccgat
     2701 cccattccac atcgacgtag ggagcgtcgg cacccgagcc gtcccacttc tcggcgagat
     2761 agatatccga ggccgcgacg ccgctcgcga tcagaccacg atcgacgttc tggcgaagca
     2821 ggaagacccg gtcacccggc acgatcccct gtgtgcgcgc tcccatcgac cacgcgccgc
     2881 gccacacctt gccggcggat gtgtccgtga tcgcggccgc caagtcctgc ggcgacagct
     2941 ccgacttctt gggattccac gtagcgatga acgttcccac ctacaccccc cgttgttgtg
     3001 gtcccatacg gtttcgcgcc gggcgtagcg acgggatgag accgcctcag cgtacccttg
     3061 cgccgacctc cgaggcgggg tcgttcgacg tcggagcgag gttcgcaaag gccctcgccg
     3121 tgtcgagcgc aatgccgcgc tcggtcgtcg aacggtcatc gccgacgatc acgtagtccc
     3181 ccacaaactc gggacgcagg ttcccgtcct cgtccaggag ccacggcatt gccaagcgcc
     3241 ggggaacccg tacgacgtct gcggtgtaag ccgtgccgaa gacgtggtcc cacaatggga
     3301 tcgtcacctg ctggttcttc gtcggatgcc cgaaatgatg ttgaaagtgg ttgatccgca
     3361 gccaccgttc ccagcggttc cgcggcccgc gcaggtgagc ggcacggtga tgccactcgt
     3421 agaagaagta cccaaccgtc caccccgcag agaaggagaa cgccacgacg gatggcaccc
     3481 accgagaccc gagccaagcg agcccggctc cgacgagcac gacccccagc caggcgagca
     3541 gcagcaccgg gtcgaacctc caactcgccc gcacgtggtg gcccaggtgc tcccgcgacg
     3601 ccagcccctt gcccttggcc cagtgaaagc tcgaatgcag gaggtactcg ccgagcgtcc
     3661 acgcaaacgc acccgccgcg aaacaaccga cgatcgcgag cgccgcaacg acgagggctg
     3721 ccatagcctg atgctaccga ggcctgttct ccgataacca gtggtcgtga tcacgaccac
     3781 tggttatcgg agaa
//
LOCUS       DAOOMA010000212         3787 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_29270_length_3787_cov_4.337889, whole genome shotgun sequence.
ACCESSION   DAOOMA010000212 DAOOMA010000000
VERSION     DAOOMA010000212.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 3787)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3787)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3787
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29270_length_3787_cov_4.337889"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            183..389
                     /locus_tag="PLP95_06790"
     CDS             183..389
                     /locus_tag="PLP95_06790"
                     /inference="COORDINATES: protein motif:HMM:NF013478.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliR"
                     /protein_id="HPB45543.1"
                     /translation="MIALFLTEILLGLASRAAPKLNIMAIGFGVKTLVTFMLLGAALP
                     LVAYFTGQLLQKSLGSMSAIVGGG"
     gene            391..1572
                     /gene="flhB"
                     /locus_tag="PLP95_06795"
     CDS             391..1572
                     /gene="flhB"
                     /locus_tag="PLP95_06795"
                     /inference="COORDINATES: protein motif:HMM:TIGR00328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhB"
                     /protein_id="HPB45544.1"
                     /translation="MAGGDKHSKTEAPTQKRKKEARKDGQVIRSEDFVTWASVLVATM
                     VMPMLVRNGSNAARDALAMMLQVARRPDEGVLVSSVGEVMTSTLGAIMPTVVIMMVIA
                     VVGNMAQVGLLLTGKPLKPKFDRLNPLKGFKRMFSVQSVWGTITALLKLTVIVAVAYT
                     TLRSSIAELVAASPRSISSLLAEAASMSISLVRTIAIAALLLAAFDYAFQRWKQYRDM
                     RMTKQEIKQESRDSEGDPHVKGRQRQLRMQASRNRMLAAIKDANVVITNPTHVAIAIE
                     YKASYGVPRVVARGVDALAMKIKAEAATHNIPQVESRPLARTLYALCRVGEEIPPELY
                     QAVATVLAFIQRLSDAQRTYVSTFDLHVPDSWSADGVTPERVSPTRRKMLERQAAKHA
                     T"
     gene            1738..>3787
                     /gene="flhA"
                     /locus_tag="PLP95_06800"
     CDS             1738..>3787
                     /gene="flhA"
                     /locus_tag="PLP95_06800"
                     /inference="COORDINATES: protein motif:HMM:TIGR01398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
                     /protein_id="HPB45545.1"
                     /translation="MKENKLGLGAAVFPFAVIAVVALMVLPIPTHLLDVLLAFNLGLA
                     MLMLLASLNVKRALDFSAFPSLLLIATLFRLGLNVSTSRLILSHGDAGEVIEAFGNFV
                     VGGSLVVGLVVFFILVIIQFVVITNGATRVAEVAARFTLDAMPGKQMAIDADLNAGVI
                     DDDEARRRREDVAGESDFYGAMDGASKFVKGDAIAAVIITAVNLIGGLLVGVLQKGMD
                     VGDAVSTYSLLTVGDGLVSQIPALLVSISSGLIITRAASSGDLGSDVMAQFARQHQAL
                     LIGGVSLIAMGLVPGLPTLPFFVVGGLLVVLSRRMSAAADASDRAAAEAPAHLEEEAD
                     PNSPAELTRALRVEPIGLELSIDLVDLVDPGSNGDLLDRVQALRRKLVEELGIVIPPV
                     RTRDNVELDLGTYVIRIHGVEVARGIAPAGKLLVIADDLSVFPGMEVLEPVFGLPSKW
                     VGPELRSMAEAMGATVIDRSSVITTHLAEVAREHAPELLSRQDVKTLVDLVRSTDSAV
                     VEDLMAAGVSLAETQQVLMSLLAEQVPIRDLSRILEVISERSRLTRDTELLVEAVRQS
                     LGPAICASNARNGHLAVITLDPLIEQGLLGSLQRNETGSFLALDPDTASRVGQAIMSQ
                     LRSAEQLGHEPVLVCAPSLRPAMRSFLARTIPHMPVLSYEELADHLTIDALGSVTLEA
                     S"
BASE COUNT          635 a         1215 c         1221 g          716 t
ORIGIN      
        1 ctaccagatt ctcggcaccg cgatgctgtt cgcgaccaac ggccacctca tcttgttcga
       61 gggactagcg cgcaccttcg atctgtcgcc ggtggacgga ttgagcttcg agcggatcgg
      121 ggccgtggtc acctcggacc ttcagacctt ccttctcgcc gcgatagatc gccgcaccga
      181 ttctgatcgc gttgttcctc accgagatcc tcctcggtct cgcatcgcgg gccgcaccga
      241 aactcaacat catggccatc ggcttcgggg tgaagacgct ggtgacgttc atgctgttgg
      301 gggccgcgct gccgctggtg gcctacttca ccgggcaact cctccagaag tcgctgggct
      361 cgatgagcgc gatcgtgggc ggaggctgac gtggcgggag gtgacaagca ttcaaagacc
      421 gaggccccga cacagaagcg caagaaggag gcccgcaagg acggacaggt catccgctcg
      481 gaggactttg tcacctgggc ttcggtgctg gttgccacga tggtcatgcc catgctggtc
      541 cgcaacggtt ccaacgcggc acgagatgcg ctggcgatga tgttgcaggt ggcccgccgc
      601 cctgatgaag gggtgcttgt gagttcggtg ggcgaggtca tgacctcgac gctgggcgcc
      661 atcatgccga cggtggtgat catgatggtc atcgccgtcg tcggcaacat ggcccaggtc
      721 ggcctcttgc tcaccggtaa accgttgaag ccgaaattcg atcgtctgaa tccgctcaaa
      781 gggttcaaac gaatgttctc cgtgcaaagc gtctggggaa cgatcaccgc gctgctgaag
      841 ctcacggtga tcgtcgcggt tgcctacacg acgctgcgtt cgtcgattgc ggagttggtc
      901 gcagcatctc cccggtcgat ctcctcgctg ctcgccgagg ctgcctcgat gtcgatctcc
      961 ctggttcgca cgatcgccat cgccgcgctc ctgctcgcag cgttcgacta cgcgttccaa
     1021 cgttggaagc agtaccgcga catgcggatg acgaaacagg agatcaaaca ggagagccgg
     1081 gattccgaag gcgatccgca cgtgaagggt cgacagcgcc agctgcgcat gcaggcgtcg
     1141 cgcaaccgaa tgttggccgc catcaaagat gcgaacgtcg tcatcaccaa cccgacccac
     1201 gtggcgatcg ccatcgaata caaggcgtcc tacggcgtcc ctcgcgtcgt ggcacgaggg
     1261 gtcgacgcgt tggcgatgaa gatcaaagcg gaggcggcga cccacaacat tcctcaggtc
     1321 gagtcgcggc ccttggcccg aacgctctac gcgctgtgtc gtgtgggaga ggagatcccg
     1381 ccggagttgt atcaggccgt tgcgacggtg ttggcgttca tccagcgcct ttccgatgcg
     1441 cagaggacct acgtatcgac cttcgacctt cacgtgccgg actcctggag cgcagacggc
     1501 gtgacgcccg agcgcgtctc gccgacgcgt cgcaagatgt tggagcgaca ggcggccaaa
     1561 catgcgacgt gagcgggtcc ctgggtcggg tggcccagga aatcatcgcg accgcctcaa
     1621 gaatcccggc gagttgccga agggaaagga ccttcatgac gaaggcgatg acccggcacg
     1681 gaggccgttg cgcggacgca gatgcgtccg attcgtttgg cacgcacggg agcattcgtg
     1741 aaggaaaaca agctcggcct cggcgcagca gtcttcccgt tcgccgtcat cgccgtcgtg
     1801 gcgctcatgg tgctgccgat tccgacgcat ctgctcgacg tgttgttggc cttcaacctg
     1861 ggcctcgcca tgttgatgtt gttggcgtcg ttgaacgtga aacgggcgct cgacttctcg
     1921 gcgttcccat cgctgctgct catcgccacc ctgttccgcc tcggcctgaa cgtcagcacc
     1981 tcacggctga tcctctcgca cggggatgcc ggcgaggtca tcgaagcatt cggcaatttc
     2041 gtcgtcggtg gctcactcgt cgtcggcctc gtcgtgttct tcatcctggt gatcatccag
     2101 ttcgtcgtca tcaccaacgg tgccacccgc gttgccgagg tcgccgcccg gttcaccctc
     2161 gacgcgatgc ccggcaagca gatggcgatc gacgccgacc tcaacgccgg ggtcatcgac
     2221 gacgacgagg cgcggcgacg gcgcgaggat gtcgccgggg agtccgactt ctacggcgcg
     2281 atggacggtg cgtcgaagtt cgtcaagggc gacgccatcg ccgcggtgat catcaccgcg
     2341 gtcaacctca tcggcggact gctggtcggc gtgctccaaa agggcatgga cgtcggcgat
     2401 gcggtgagca cctactcgtt gctcaccgtc ggcgacggcc ttgtgtcgca gatccctgca
     2461 ctgttggtgt cgatttcctc gggtctgatc atcacccgag cagcgagcag cggcgacctc
     2521 ggtagcgatg tgatggcgca gttcgcccgt cagcatcagg cgttgctgat cggtggcgtc
     2581 tccctcatcg ccatgggctt ggtgcccggg ctgccgaccc tgccgttctt cgtcgtcgga
     2641 gggctgctgg ttgttctgtc tcgcaggatg tccgccgctg ccgatgcatc ggaccgggcg
     2701 gccgccgagg ctccggccca cctcgaagag gaggccgatc cgaactcgcc ggccgaactg
     2761 acccgggcac tgcgcgttga accgatcggg ctggagctgt cgatcgacct ggtcgacctc
     2821 gtcgatcccg ggtcgaacgg cgacctgttg gaccgggtgc aggcgcttcg tcgaaagctc
     2881 gtcgaggaac tgggcatcgt cattccgccg gtgaggacgc gtgacaacgt cgagctcgac
     2941 ctcgggacct acgtgatacg gattcacggg gtggaagtgg cgcgagggat cgcccctgcg
     3001 ggcaagcttc tcgtgatcgc cgacgacctc agcgtgttcc ccggtatgga agtgctcgaa
     3061 cccgtcttcg ggcttccatc gaagtgggtg gggccagagc tgcggtcgat ggccgaagcg
     3121 atgggcgcca ccgtcatcga ccgttcgtcg gtgatcacga cccatctggc cgaggtggct
     3181 cgcgagcacg cccccgagct gttgagccgc caagatgtca agaccctcgt cgacctggtc
     3241 cgctcgaccg acagcgccgt ggtcgaggac ctgatggccg cgggggtgag cctcgccgag
     3301 acccagcagg tactcatgag tctgctcgcc gaacaggttc cgatccggga tctgtcgcgg
     3361 atcctcgagg tcatcagcga gcgttcgcgt ctgacccgcg acaccgagtt gttggtcgag
     3421 gccgtgcgtc aatcgctggg cccggcgatc tgcgcgtcga acgcccgcaa cggtcacctc
     3481 gcggtcatca ccctcgaccc gctcatcgaa caggggttgc tggggtcgct gcaacgcaac
     3541 gagacagggt cgtttctcgc cctcgacccc gacaccgcgt cgcgggtcgg ccaagcgatc
     3601 atgagccagc ttcgcagtgc cgaacaactc ggccatgaac cggtgctcgt ctgtgcgccg
     3661 agtctacggc ccgccatgcg aagcttcctc gcccggacca ttcctcacat gccggtgttg
     3721 tcctatgaag aactcgccga tcacctcacg atcgacgcgc tcggctcggt gaccctggag
     3781 gccagcc
//
LOCUS       DAOOMA010000213         3785 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_29295_length_3785_cov_2.844772, whole genome shotgun sequence.
ACCESSION   DAOOMA010000213 DAOOMA010000000
VERSION     DAOOMA010000213.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 3785)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3785)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3785
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29295_length_3785_cov_2.844772"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(101..481)
                     /gene="gcvH"
                     /locus_tag="PLP95_06805"
     CDS             complement(101..481)
                     /gene="gcvH"
                     /locus_tag="PLP95_06805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015798543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HPB45546.1"
                     /translation="MEFPADLRYSTDHEWVRVEGGNVRLGITDFAQDNLGDVVYVELP
                     EQGATVEVGASFSEVESTKSVSDIYSPVNGTVVEINEALEADATLLNSDPYGAGWICV
                     IALDDPSQLAGLLDADGYRALVSD"
     gene            complement(523..2373)
                     /locus_tag="PLP95_06810"
     CDS             complement(523..2373)
                     /locus_tag="PLP95_06810"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HPB45547.1"
                     /translation="MRTSRVAGAFVIAGLIGEVFGGAGGVVGAGAAPVAASVASPVAA
                     SVAASVDVPRDQGSEPVELILSGVDDPTPAEIGRLAGADVEVVATIGQLGAVKIAVDP
                     DDVAAVIAGLDSAHEVGDVSAPGRVTAARTPTDPGWARQFWMPDQGFTRAWDRTVGSS
                     NVVIATVDTGVNTTPDLAGRVIAGADFTGTSVTGVGRDGDNDPQRHGTLTAELAAAAG
                     DDGYGIAGACWTCRVLPVKVLDADGEGDTWAVGEGIVYAVDHGAHVINLSLGGATDDP
                     VVQRAVRYAESRNVVVVAAAGNDYDEAGSAARYPAAYPTVVGVGAYTRLGDGPPSTSQ
                     RGPWYELAAGYCRYDRYFAVDNICGTSFSAPYVSGTVGLMRSLVPDASAAQYRAAMYN
                     SAVAVPSLTGSGLYRYGALSADGALTRIAATVPAAPVPAASERSAPTVAVSAPAGYSS
                     GTVTLEVSNSDDVGVTNLAVYVGDTLVFAQSAPPARFPVAVDTTRFADGVHRVAVVAY
                     DAAGNWGGAETAMAIDNHLPIALLYSPGTSTTVRRHLDIGVYTTDGSGIMGTFLIVND
                     QVVGGYVGSGFYSIRVPIRRNGRYNIVALTVDNAGRISASNWSRVNGRTR"
     gene            complement(2370..3050)
                     /gene="pgsA"
                     /locus_tag="PLP95_06815"
     CDS             complement(2370..3050)
                     /gene="pgsA"
                     /locus_tag="PLP95_06815"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HPB45548.1"
                     /translation="MDQHEHHLIYRPEVEGLGDMQVDRILTVPNAISLVRLLCIPVFL
                     WLLFGMDSPGWAGLLLGLLAATDWVDGYIARRFDQTSNFGKMFDPVVDRIMLVVGIVA
                     VIISGSAPLWFSALVLIREVLVSGWVILITSLGAKRMDVTWWGKCGAFANMGAFPAFL
                     IADQRSLGEGWHTGWTLFAYALAIPGVVFSLFAAGQYFVRGRVALKEGRLKRQLAAAD
                     GEATGEVR"
     gene            3112..3555
                     /locus_tag="PLP95_06820"
     CDS             3112..3555
                     /locus_tag="PLP95_06820"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HPB45549.1"
                     /translation="MTDNAPSAPDSVFTLIIRGDLPGRFVWRDERVVAFLTIAPITAG
                     HVLVVPVDQVDKWTDLPNDLWLELNSVAIEVGRAIEDAFDCVRVGTIIAGLEVPHCHL
                     HLLPINAESDLSFAKANPNVPAEELDAAAEAIRAALRARGHATPG"
BASE COUNT          641 a         1354 c         1212 g          578 t
ORIGIN      
        1 ctcacgccgc tcgagcgccg aaccgcaggc aggacagaag ttggcatcgg ccccaaccgc
       61 ggagccgcat ttggggcact gcgacgcctc ggccatgacc tcagtcgctc accagcgccc
      121 ggtacccgtc ggcgtcgagc aggccggcga gttgtgacgg gtcatcaagc gcgatgacac
      181 agatccaccc ggcgccatag gggtcgctgt tgagcagcgt cgcgtcggct tcgagcgcct
      241 cgttgatttc gaccaccgtg ccgttgaccg gcgagtagat gtcggacacc gacttggtcg
      301 attccacctc gctgaacgac gcccccacct cgacggtggc gccctgctct ggcaactcga
      361 catacaccac gtcgccgagg ttgtcctggg cgaaatcggt gatgccgaga cggacattgc
      421 cgccttcgac ccgaacccac tcgtggtcgg tcgaatagcg aaggtcagcg ggaaactcca
      481 tggccggaga ctctagtgga cggcggctga gcgcggattg cctcaacggg ttcggccgtt
      541 gaccctcgac cagttcgatg cgctgatacg cccggcattg tcgacggtca acgcgacgat
      601 gttgtagcgg ccgttgcggc gaatgggcac ccggatcgaa tagaacccgc tcccgacata
      661 tccgccgacc acctgatcgt tgacgatcag gaaggtgccc atgattcccg aaccgtcggt
      721 cgtgtagacc ccgatgtcga gatggcgtcg caccgtcgtc gaggttcccg gcgaatacag
      781 caacgcgatc gggaggtggt tgtcgatcgc catcgccgtc tcggcgccac cccagttgcc
      841 cgccgcgtcg taggccacga ccgcgacccg atgcacaccg tcggcgaatc gggtggtatc
      901 gaccgcaacg gggaaccgcg ccggcggggc ggactgggcg aacaccaggg tgtcgcccac
      961 atagaccgcg aggttggtga cgccgacgtc gtcggagttg gacacctcga gcgtcacggt
     1021 cccagacgaa taccccgccg gagccgagac cgcgacggtc ggggcgctgc gttcgctggc
     1081 cgcgggcacc ggcgctgccg ggaccgtcgc ggcgattcgc gtcagtgccc cgtcggccga
     1141 caacgccccg tagcggtaca ggcctgaacc ggtcagcgac ggtacggcga ccgccgagtt
     1201 gtacatcgct gcgcggtact gcgcggccga ggcatccggc acgagactgc gcatcaaccc
     1261 gaccgttcca ctcacatacg gcgcgctgaa gctggttcca cagatgttgt cgacggcgaa
     1321 gtagcggtcg tagcggcagt accccgccgc gagttcgtac cacgggccgc gctggctcgt
     1381 cgacgggggg ccgtcgccga gccgcgtgta ggcgccgacc ccgaccacgg tgggataggc
     1441 agccggatac cgagctgccg aaccggcctc gtcgtagtcg ttgcccgccg cggccaccac
     1501 gacgacgttg cggctctcgg cgtagcgcac cgcccgctgc acgaccgggt catcggtcgc
     1561 cccaccgagg ctgaggttga tgacgtgggc gccgtggtcg acggcataga cgatgccctc
     1621 gcccaccgcc caggtgtcgc cctcgccgtc ggcgtcgagc accttgaccg gaaggacccg
     1681 acaggtccag cacgctccgg cgatcccgta tccgtcgtcg cccgcagcag ccgccagttc
     1741 cgcggtgagc gtgccgtgac gctgggggtc attgtcgcca tcgcgtccga cgccggtcac
     1801 cgaggtgccg gtgaagtcgg ctccggcgat gacgcgtccc gcaagatcgg gggtcgtgtt
     1861 gacccccgtg tcgaccgttg cgatcaccac gttcgatgag ccgacggttc ggtcccacgc
     1921 ccgggtgaat ccctgatccg gcatccagaa ctgccgagcc cagcccggat cggtcggcgt
     1981 gcgagcggcg gtgacgcggc cgggggccga cacgtcgccg acctcgtggg ccgagtcgag
     2041 acctgcgatc accgccgcga cgtcgtcggg gtcgaccgcg attttcaccg cgccgagttg
     2101 tccgatcgtg gcgaccacct cgacgtcggc ccccgccagg cgcccgattt ccgctggagt
     2161 gggatcgtcg actccgctga ggatgagctc gaccggttcc gatccctgat cccgcggcac
     2221 atctaccgat gcggccaccg atgcggccac cggcgaggcc accgatgcgg ccaccggcgc
     2281 tgccccggca cccacgactc ccccggcacc gccgaacact tcaccgatga gtccggcgat
     2341 gacgaacgcg ccggcgacac gactcgtcct caccggacct ccccggtcgc ctcgccgtcg
     2401 gccgccgcca actggcgctt caggcgccct tccttcaacg ccacgcggcc ccggacgaag
     2461 tactggcctg cggcgaacag cgagaacacc accccgggaa tggcgagcgc gtaggcgaac
     2521 agggtccatc ccgtgtgcca gccctcgccg agtgatcgtt gatctgcgat caggaacgcc
     2581 ggaaacgctc ccatgttggc gaacgctccg cacttacccc accaggtgac gtccatgcgc
     2641 ttcgcgccga gcgaggtgat gaggatcacc catccggaca cgagaacttc gcggatcaac
     2701 accagggcgc tgaaccacag cggagccgac ccggagatga tcaccgcgac gatgccgacg
     2761 accaacatga tccggtcgac gaccgggtcg aacatctttc cgaagttcga ggtctgatcg
     2821 aaccgccggg cgatgtagcc gtcgacccaa tcggtggccg ccagcaggcc gagcaagagc
     2881 cctgcccagc cgggcgagtc catgccgaac agcagccaca gaaacaccgg aatacacagc
     2941 aaccgcacga gcgagatcgc attcgggacc gtcaggatcc ggtcgacctg catgtcgccg
     3001 aggccctcga cctccggtcg gtagatcaga tggtgctcgt gctggtccac ggccttgagg
     3061 ttacggcatc gacccatccc ctcacgcagc gctccaccta cgattcgcta catgaccgac
     3121 aacgccccca gcgctcccga ctccgtgttc accctcatca tccgcggcga cctgccgggg
     3181 cgcttcgtct ggagagacga gcgcgtcgtc gcgtttctca cgatcgcacc gatcacggcg
     3241 ggccacgtgc tcgtcgtgcc ggtcgaccag gtggacaagt ggaccgatct acccaacgac
     3301 ctgtggttgg agctgaactc cgtggccatc gaggtcggac gcgccatcga ggacgcgttc
     3361 gactgcgtgc gggtcggcac gatcatcgcc ggcctcgagg tgccccactg tcacctccac
     3421 ctgctgccga tcaacgccga atcggacctg tccttcgcca aggcgaatcc gaacgtcccg
     3481 gcggaggagc tcgatgcggc cgccgaggcc atccgcgcag cgctgcgcgc ccgcggtcac
     3541 gccacacccg gctgacggct tctccgactg accgcttctc ctaccgacgg cttctcctac
     3601 cgacgaatcc tcacgcctcc tcggtcaact cgacggggtc gatgaggtgt gcacccttgt
     3661 tgcgggcgtt gttgacctcg gtcgataccg gatgcatcgt caacaccccc gtctccgccg
     3721 gcaccatgag cggcgcgacc gcggcggcgt cggtgatcgc agggtccagc caggtggcga
     3781 aatcg
//
LOCUS       DAOOMA010000214         3769 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_29470_length_3769_cov_3.823640, whole genome shotgun sequence.
ACCESSION   DAOOMA010000214 DAOOMA010000000
VERSION     DAOOMA010000214.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 3769)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3769)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3769
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29470_length_3769_cov_3.823640"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..364
                     /locus_tag="PLP95_06825"
     CDS             <1..364
                     /locus_tag="PLP95_06825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB45550.1"
                     /translation="LAFVRARHYTEIIDGAEVTEPTGDLGRQQRQQLFIRTVLKTAGA
                     ERNPITLARIASGAASGVKVDQNIGFGDLLSLARRLGSGDPETRVLPTENARKGDAAV
                     LVLRDAEALGVLMEFGAG"
     gene            491..1048
                     /locus_tag="PLP95_06830"
     CDS             491..1048
                     /locus_tag="PLP95_06830"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020379025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HPB45551.1"
                     /translation="MSSTPAPAADGSSPRQTKFSAPMPMTVDPDKQYTATIDTSAGTM
                     VAFLYPNKAPKTVNNFVNLARYHYYDGLIFHRVIQGFVIQGGCPEGSGRGGPGYRFED
                     ELPEPGKYEIGSLAMANAGPNTNGSQFFVISGPQGVTLPPRYALFGKLISGLDVLEAI
                     QTTSTDRGDRPVDDVVINSITITEE"
     gene            1053..1853
                     /locus_tag="PLP95_06835"
     CDS             1053..1853
                     /locus_tag="PLP95_06835"
                     /inference="COORDINATES: protein motif:HMM:NF015094.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/UbiB family protein"
                     /protein_id="HPB45552.1"
                     /translation="MRRRSLVAVLAGGGVLGWFVRRRRVGGERSGNPTGAFIGTSTKL
                     ATGRGDRTAELAKLGAKSGASFVKLQAGSRFASDERKAELQERFQLETAEQVASVLGN
                     MKGAVMKLGQMASYLDGGLPEPVRDALAQMQQDAPPMSYSLVQEVVRSELGAEPDELF
                     AYFDHEPMAAASIGQVHRARTHDGRDIVLKVQYPGVDEAIRVDLDNSDLLFGAMSFLF
                     SGLDRTAGERVARSPRGGTRLHDRGRTPASVRRPLPRAPDDSRSRCRR"
     gene            1738..2571
                     /locus_tag="PLP95_06840"
     CDS             1738..2571
                     /locus_tag="PLP95_06840"
                     /inference="COORDINATES: protein motif:HMM:NF015094.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/UbiB family protein"
                     /protein_id="HPB45553.1"
                     /translation="MRDRLVEELDYTIEAAHQRRFADHYRGHPTIHVPDVVDEFSTRR
                     VLCTEFASGSKWSEVLAWSQDERNLIAETLYRFAFGGIYRLGAFNGDPHPGNYVFNPG
                     GQVTFLDYGLCKVFTPHEIAEFEKVIKTMVFDRVPERIVAVWEDLGVLKRADKLDPNL
                     VVEYFSFFFEAVLKPGQRQITAEYASDSIRRYFDLSGPFAAVMKSVTLPSFMVVIQRI
                     NLGLYALFGELEAVRDWRGLAEEIWPFVDADPTTPMGHEIRRWELDRGLRSPGHHHRE
                     P"
     gene            2568..3077
                     /locus_tag="PLP95_06845"
     CDS             2568..3077
                     /locus_tag="PLP95_06845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB45554.1"
                     /translation="MTVAGSAAACGLGLAIVFLAAALSKMGRRSAVTESFAAMGLVAP
                     EALAVLVPILELATAVLLVLWPLGGAGLALLLLVAFTVVLARLVASGVSVGCACFGST
                     SNAPVSFVELVRNAGLMMMAIAVITMGDFGESLGWPGVADVLFVAALMGVGALVLRLL
                     RNLDRPGGE"
     gene            complement(3121..>3769)
                     /locus_tag="PLP95_06850"
     CDS             complement(3121..>3769)
                     /locus_tag="PLP95_06850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HPB45555.1"
                     /translation="ATTLAEALALFGLEAHVRDPAVIRQAYLRTIRETHPDRNRAADA
                     TRRSAALTDAYRLVTSRLDEPVAGPEDSPAAASRNTGRTTSRSTSRSASRAPSAPPRP
                     SASETHEIDGWVRIIADDTIELGAPSDLAFRWLELASHRAGDITYLDRPSAMLQVLME
                     FVGYPLCYVVFDLQGRAANGSTEVFCTIASLDDRDPPPIAAVTEFMAAQLDDVLD"
BASE COUNT          589 a         1216 c         1300 g          664 t
ORIGIN      
        1 gctggcgttc gtgcgcgctc gtcactacac cgagatcatc gacggagccg aggtgaccga
       61 accgactggg gacctcggtc gccaacagcg ccagcagctc ttcatccgga ccgttctgaa
      121 aaccgccggg gccgagcgca acccgatcac cctcgccagg atcgcgagcg gcgcggcatc
      181 gggggtcaag gtcgaccaga acatcgggtt cggcgacctg ttgagtctgg cccgccgcct
      241 cggatccggc gaccccgaga cccgggtgtt gccgaccgag aatgcgcgga aaggcgatgc
      301 tgcggtgctc gtgctccgcg acgccgaggc cctcggcgtg ctcatggagt tcggggccgg
      361 atagccccgg cgcgcgctgg ccgtcggcgc gcgctggccg tcggtgcgcg ctggccgtcg
      421 gtgcgcgctg agcgtcgcag tcggggcact aggttggcgt gcgcctccac acgacgacga
      481 aaggcactcc atgagttcga ctccggcacc cgccgccgac ggttcctccc cccgccagac
      541 caagttctcg gcccccatgc cgatgacggt cgaccccgac aagcagtaca cggcgacgat
      601 cgacacctcg gccggcacga tggtggcgtt tctctatccc aacaaggcgc ccaagaccgt
      661 caacaacttc gtcaatctcg cccgctacca ctactacgac ggcctgatct ttcaccgcgt
      721 catccaaggg ttcgtcatcc agggcggttg cccggagggt tcgggtcgcg gcggccccgg
      781 ctaccgcttc gaagacgaac ttcccgagcc tggcaagtac gagatcggct cgctcgcgat
      841 ggccaacgcc ggcccgaaca ccaacggttc gcagttcttt gtgatctccg gccctcaggg
      901 tgtgacgctg ccgccgcgtt acgccttgtt cggcaagctc atcagcggcc tcgacgtcct
      961 cgaggcgatc cagaccacct cgaccgatcg cggcgatcgc ccggtcgacg acgtggtcat
     1021 caactcgatc accatcaccg aggagtgagg tgatgcgccg gcgtagcctt gtcgcagtgc
     1081 tcgccggcgg cggagttctc ggctggttcg ttcgccgccg ccgtgtcggc ggcgaacgaa
     1141 gcggcaaccc gaccggtgcg ttcatcggca cctccaccaa actcgccacg ggccgcggcg
     1201 accgaaccgc ggagttggcc aagctgggag cgaaatcggg cgccagtttc gtgaagctgc
     1261 aagcgggctc gaggttcgct tcggacgaac gcaaggccga actgcaggag cgctttcagc
     1321 tcgagaccgc cgagcaggtg gcctcggtgc tcggcaacat gaagggcgcc gtcatgaagc
     1381 tcggccagat ggcgagctac ctcgacggcg gcctgcccga gccggtgcgt gacgccttgg
     1441 cgcagatgca acaggacgca ccgcccatga gttattcgct cgtgcaggag gtggtgcggt
     1501 ccgaactcgg cgcggagccc gacgagctgt ttgcctactt cgaccacgag ccgatggccg
     1561 ccgcgtcgat cgggcaggtg caccgcgccc ggacccacga cggacgagac atcgtgttga
     1621 aggtgcagta ccccggcgtc gacgaggcga ttcgtgtcga tctcgacaac tccgacctgc
     1681 tgttcggggc gatgagtttc ctgttcagcg gactcgaccg aaccgctggt gaacgagttg
     1741 cgcgatcgcc tcgtggagga actcgactac acgatcgagg ccgcacacca gcgtcggttc
     1801 gccgaccact accgcgggca cccgacgatt cacgttcccg atgtcgtcga tgagttctcg
     1861 acgcgccggg ttctgtgcac cgagttcgct tccgggtcga agtggtccga ggtgctggcc
     1921 tggtctcagg acgaacgcaa cctgatcgct gaaaccctgt atcgcttcgc gttcggcggc
     1981 atctatcgcc tcggggcgtt caacggtgat ccgcatcccg gcaactacgt gttcaacccc
     2041 ggcgggcagg tcaccttcct cgactacggc ctgtgcaagg tgttcactcc gcacgagatc
     2101 gccgagttcg aaaaggtcat caagacgatg gtgttcgatc gcgtccccga acgcatcgtg
     2161 gccgtgtggg aggacctcgg ggtattgaaa cgggccgaca aactcgaccc gaatctcgtg
     2221 gtcgagtact tctcgttctt cttcgaggcc gtgctcaagc cggggcaacg acagatcacc
     2281 gccgagtacg catcggattc gatccggcgg tacttcgacc tgtcgggccc gttcgcggcg
     2341 gtgatgaaga gcgtcacgct gccgagcttc atggtcgtga tccaacgcat caacctcggg
     2401 ctgtacgcac tgttcggtga gctcgaggcg gtgcgcgact ggcgtggtct cgccgaggag
     2461 atctggccgt tcgtcgacgc cgacccgacc acgccgatgg gccacgagat ccgacggtgg
     2521 gaactcgatc gcggcttgcg ctcacctggc catcaccatc gcgaaccatg accgtcgcgg
     2581 gcagcgcggc ggcctgcggg ctcggtctcg ccatcgtctt tttggcagcg gcgctatcga
     2641 agatgggtcg gcgatctgcg gtgaccgagt cgttcgccgc aatggggctg gtcgcccccg
     2701 aggcgttggc cgtgctcgtg cccatcctcg agctggcgac cgcggtgctg ctggtgctgt
     2761 ggccgttggg tggggccgga ctcgcactgc tgttgttggt ggccttcacc gtggtgctcg
     2821 cacgactcgt cgcatccggg gtctcagtgg ggtgcgcgtg tttcggatcg acctcgaatg
     2881 ccccggtgag ctttgtggag ttggtgcgca acgcggggct gatgatgatg gcgatcgcgg
     2941 tcatcaccat gggtgacttc ggagagtcgc tcggatggcc cggcgtcgcg gacgtgttgt
     3001 tcgtggcggc gctgatgggg gtcggcgccc tggtgcttcg gctgttgcgg aacctcgatc
     3061 ggcccggcgg cgagtgagcg ctcggcgcgg ctcgtttcgg ctcgtttcgg ctcggctctg
     3121 tcagtccaag acgtcgtcga gctgcgcggc catgaactcg gtcaccgctg cgatcggggg
     3181 tgggtcgcgg tcgtcgaggg aggcgatcgt gcagaacacc tcggtggatc cgttcgcggc
     3241 ccggccttgc aggtcgaaca ccacgtagca caacggatag ccgacgaact ccatcagtac
     3301 ctgcagcatc gctgaggggc gatccagata ggtgatatcg ccggcacgat gcgaggcgag
     3361 ctccagccac cggaaggcaa ggtcggacgg tgcgccgagt tcgatggtgt cgtcggcgat
     3421 gatgcggacc cacccgtcga tctcgtgagt ctcgctggcg gatggccgcg gcggtgcgct
     3481 cggcgcgcgt gacgcagagc gcgacgttga gcgcgacgtt gtgcgcccag tgttccgcga
     3541 ggccgcagcg ggggagtcct cgggtccggc gacgggctcg tcgagacggg aggtcacgag
     3601 acggtaggcg tcggtcaggg ccgccgaacg tcgggtggcg tcggcggcgc ggttgcggtc
     3661 cgggtgcgtt tcgcgaatcg tgcgcaggta cgcctgacgg atcacggcgg ggtcgcgcac
     3721 atgtgcttcg aggccaaaca gcgcgagcgc ttcggcgagc gtggtcgca
//
LOCUS       DAOOMA010000215         3766 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_29509_length_3766_cov_3.760981, whole genome shotgun sequence.
ACCESSION   DAOOMA010000215 DAOOMA010000000
VERSION     DAOOMA010000215.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 3766)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3766)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3766
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29509_length_3766_cov_3.760981"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1656)
                     /locus_tag="PLP95_06855"
     CDS             complement(<1..1656)
                     /locus_tag="PLP95_06855"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF024642.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HPB45556.1"
                     /translation="MGDSEASNPRWARGLNAAQYAAAVSDADALLIVAGAGTGKTGTL
                     AARVARLIEEGIPPNRILLLTFTRRAAQEMLARAGRLSDGVATAQVWGGTFHSVANRL
                     LRHYGRGVGLDPNFTVLDQADALELFGIVRAAAVDTAGTRFPKKETIAAVYDRVVGTQ
                     RPLGEVVRAEYPWVVGHLEALPPLFVEYTRRKRSRRVLDYADLLLFWRAMLVAPEVGP
                     EVASRFDHVLVDEFQDTDPVQADIVHSLADFGCKLTVVGDDAQSIYGFRAATVENMWR
                     FVDRRPGAEQVTLEENYRSVAPILAVANAVLAQSDRHFDKELRARRSGERRPVLVSCH
                     DELSQARVVVDNILELREEGMLLSDQAVLFRAGYHSDLLELELGRRDVPYVKWGGLKF
                     LEAAHIKDLVSLLRVLDNPWDELAWSRVLRTLPGVGPASVRSISAAIGLDALGDVEFD
                     GARGDSAALAGGARTPLDRFLRDEIAVPSAAREEFLTLREALSQCIGRSADGVGQEPP
                     VAAQVERLIEWCNPSFDRRYERAEVRAGDLQQLAVLAESYESRS"
     gene            1718..2176
                     /locus_tag="PLP95_06860"
     CDS             1718..2176
                     /locus_tag="PLP95_06860"
                     /inference="COORDINATES: protein motif:HMM:TIGR00738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HPB45557.1"
                     /translation="MHVTAKADYAMRAMIEIAASPEGRAKAEQIAEAQDIPLGFLRGI
                     LTDLRRSGLIVSQRSANGGFRLARDAASISIGDVVRSVEGPLAQVRGLKPTELDYPGA
                     AAPLRDVWIALRCNLRAIVDHTTIADVASGDLPALVRDLVADPSSLIENF"
     gene            complement(2199..2666)
                     /locus_tag="PLP95_06865"
     CDS             complement(2199..2666)
                     /locus_tag="PLP95_06865"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000932331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008175 - tRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (cytidine(34)-2'-O)-methyltransferase"
                     /protein_id="HPB45558.1"
                     /translation="MFHIVLVAPQIPPNTGNVIRLCANVGASLHLVRPLGFDIDDASL
                     RRAGLDYHEFVTMRVHDDLAQCRAAIGDGPSWYGLSRHGRRTYTDVAYSPGDVLVFGS
                     EASGFTPEVRAGFGERLISLPMRPDNRSLNLSNSVAVVAYEAWRQNGFAGAQD"
     gene            2738..3721
                     /locus_tag="PLP95_06870"
     CDS             2738..3721
                     /locus_tag="PLP95_06870"
                     /inference="COORDINATES: protein motif:HMM:NF024117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HPB45559.1"
                     /translation="MSEPEHRVPADPHFNDAQAEVLARLGAPRSQRPTFEPGLKEHLR
                     AELEKRLAATIRLLGDDLDDTIWLSKHKLEKVHGCETRYLAEEDLEFSWSVPVARGIV
                     AHKAIEVDVTTTREWAPLDLIDEAIARLIAEAKSVGEWLSTAEAGDLDMVRAEANNAL
                     VTFQECFPPLDRKWRPATEVPMRADFFEGRIVLSGKPDLTIGQADGHVAGKVIVDFKT
                     GRRSPAHVEDLRFYALLDALRVGVPPRLLVSYYLDSASMQTEDVDEQVLHSAVLRTAS
                     AAHKIVELMIGEREPTLVGSPSCRWCPLLTSCDTGRSHLSGEIADDHSWEP"
BASE COUNT          693 a         1338 c         1143 g          592 t
ORIGIN      
        1 actgcgcgac tcatacgact cagccagcac cgcgagctgc tgcaggtcac ctgcccgcac
       61 ctcggctcgt tcgtaccgac gatcgaaact ggggttgcac cactcgatga gccgctcgac
      121 ctgcgctgcg accggcggct cttgcccgac accgtcagcg ctccgcccga tgcactgcga
      181 caacgcctcc cgcagggtca ggaactcctc gcgagccgca cttgggactg cgatttcatc
      241 ccgtaggaac cggtccagcg gggttcgagc gccgccagcg agcgcagcgc tgtcgcctct
      301 tgcaccatcg aactcgacat cacccagagc gtcgagtccg atcgctgctg atattgaacg
      361 caccgacgcc ggtccgaccc ccggcagggt gcgcaacaca cggctccagg ccagctcgtc
      421 ccacgggttg tcgagcaccc gcaacaacga caccaggtcc ttgatatgtg cagcctcgag
      481 aaacttcaac cctccccatt tcacataggg aacgtcccgg cgaccgagtt caagctccag
      541 caggtcggag tggtatcccg ctcgaaacag cacggcctgg tccgacagca acatgccctc
      601 ttcgcgaagc tccaagatgt tgtcgaccac cacgcgggcc tggctcaact cgtcgtgaca
      661 cgacaccagc acgggtcgac gctcgcccga acgacgagcg cgtagctcct tgtcgaagtg
      721 tcggtccgac tgggccaaca cggcattggc cacggccaga atgggcgcca ccgagcgata
      781 gttctcctcc agggtgacct gttcagcccc cggacgacgg tcgacgaacc gccacatgtt
      841 ctccacggtc gccgcccgga acccgtagat cgactgcgca tcgtcaccga ccacggtgag
      901 tttgcatcca aagtccgcca gcgagtgcac gatgtccgcc tgcaccgggt cggtgtcctg
      961 aaactcgtcg acgagcacgt gatcgaagcg cgaagcaacc tccggaccca cctcgggggc
     1021 aaccaacatc gcccgccaga acaacaacag atccgcgtaa tccagcaccc gccgcgaccg
     1081 cttgcggcgc gtgtactcga cgaacagcgg gggaagcgcc tccagatgcc ccaccaccca
     1141 gggatactcg gcgcgaacca cctcgccgag cggacgctgc gtgcccacca cgcggtcgta
     1201 gacggcggcg atcgtctcct tcttgggaaa gcgggttccc gccgtgtcga ccgcagccgc
     1261 acgaacgata ccaaagagct cgagtgcatc ggcctgatcg agcaccgtga agttggggtc
     1321 gagtccgact cctcgcccgt agtggcgcaa gagccgattg gcgaccgaat gaaaggtacc
     1381 gccccacacc tgcgcagtgg cgaccccgtc gctcagccga ccggcccgcg ccaacatctc
     1441 ctgggcggct cggcgggtga aggtcagcaa caggatgcga ttcggcggga tgccttcctc
     1501 gatcagccgg gcaacccgcg ccgcgagcgt gccggtcttg ccggtgccgg caccggccac
     1561 gatgagaagt gcgtcggcat cggacaccgc agcggcgtat tgcgcggcat tgaggcctcg
     1621 cgcccaacga gggttcgatg cttcgctatc gcccaaatcc ggttctacga acattcgttc
     1681 ggcagtctag tgcgtcgggg tctgccagac tgtcgtcgtg catgtcaccg ccaaagccga
     1741 ctacgcgatg cgcgccatga tcgagatcgc cgcctcaccg gaaggccgag cgaaggccga
     1801 acagatcgcc gaggcacaag acattccact cgggttcctg cgcggcatcc tcaccgacct
     1861 gcgccgatcc ggcctcatcg tgtcccagcg aagtgccaac ggcgggttcc gcctggcccg
     1921 cgacgcggcc tcgatctcga tcggcgacgt cgtgcggtcg gtcgaaggcc ccctcgctca
     1981 agttcgtggc ctcaagccca ccgagttgga ctaccccggc gccgccgccc ccctacgcga
     2041 tgtgtggatc gctctgcgct gcaacctacg agcgatcgtc gaccacacga ccatcgctga
     2101 tgtggcgtcg ggcgacctcc ccgcgctcgt tcgcgatctg gtcgccgatc cgtcctcgtt
     2161 gatcgagaac ttctgacctc cgcatcccga ccagcgcttc agtcctgggc tccggcgaag
     2221 ccgttctgtc tccacgcttc gtaggccacc accgccacgc tgttggagag gttgaggctg
     2281 cggttgtccg gccgcatcgg cagcgagatg agtcgctcgc cgaaccccgc acgcacctcc
     2341 ggcgtgaacc ccgacgcctc cgaaccgaac accaggacat cgccgggcga gtacgcgacg
     2401 tcggtgtagg tgcgacgtcc gtgacggctc aggccgtacc acgacggacc gtcgccgatc
     2461 gcggcgcgac actgagcaag gtcgtcgtgc acccgcatcg tcacgaactc gtgatagtcg
     2521 aggcccgccc ggcgcaacga cgcatcgtcg atgtcgaagc cgagcggccg gacaaggtgc
     2581 agcgaggctc cgacattggc gcacaatcgg atcacgttgc cggtgttcgg aggaatctgg
     2641 ggcgcgacca gcacgatgtg aaacatgatg cccatgccta ccgggtcggt ggcgactcgg
     2701 gcaacgacga tccgttgtcg tctctacact cgattcgatg agcgagccgg agcatcgagt
     2761 cccagcggac ccgcatttca acgacgctca ggccgaggtt ctcgcccgtc tcggcgcgcc
     2821 gagatcgcaa cggccgacgt tcgagcccgg gctgaaggaa catctacgag ccgagttgga
     2881 aaagcggctc gctgcgacga ttcgcctgct cggagacgac ctcgacgaca caatctggct
     2941 ctccaaacac aagttggaga aggtgcacgg ctgcgagacc cgctatctcg ccgaggagga
     3001 ccttgagttc tcctggagcg ttcccgtggc ccggggcatc gtcgcccaca aggcgatcga
     3061 ggtcgacgtc acaacgaccc gagagtgggc accactcgac ctcatcgacg aggccatcgc
     3121 ccgcctcatc gccgaggcca agtcggtcgg cgaatggctg tccaccgcgg aggcgggcga
     3181 cctcgacatg gtgcgcgccg aggccaacaa cgcgctggtc accttccagg agtgcttccc
     3241 gccgctcgat cgcaagtggc gtccggcgac cgaggttccg atgcgagccg acttcttcga
     3301 gggccgcatc gttttgagcg gcaagcccga cctgacgatc ggccaggccg acggccacgt
     3361 cgccggcaag gtcatcgtcg acttcaagac cggccgacgc tccccggccc acgtcgagga
     3421 tctgcgcttc tacgcactgc tcgacgctct acgtgtcgga gttcctccgc gactcctggt
     3481 gagctactac ctcgacagcg catcgatgca gaccgaggac gtcgacgaac aggtcctgca
     3541 ctcggcggtc ctacgaaccg cgtccgcggc gcacaagatc gtcgagctga tgatcggcga
     3601 acgcgagccg accctcgtcg gctccccgtc gtgtcgatgg tgcccgctgc tgacgtcctg
     3661 cgataccgga cgatcacacc tctcggggga gatcgccgac gaccactcct gggagccctg
     3721 acccagcgat cccgaccggg gagccgagtt tggccgctac gaatcg
//
LOCUS       DAOOMA010000216         3765 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_29521_length_3765_cov_3.800270, whole genome shotgun sequence.
ACCESSION   DAOOMA010000216 DAOOMA010000000
VERSION     DAOOMA010000216.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 3765)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3765)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3765
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29521_length_3765_cov_3.800270"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..772)
                     /locus_tag="PLP95_06875"
     CDS             complement(<1..772)
                     /locus_tag="PLP95_06875"
                     /inference="COORDINATES: protein motif:HMM:NF014564.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HPB45560.1"
                     /translation="MPQAHDGAAHGARVLEGVRVLEVAEHTFVPAASALLADWGAEVI
                     KIEHVTRGDAMRGLASTGVAELGGEVHALLEHSNRGKKSLGLDLTQPEGLEVLYRLAA
                     TADVFITNKLPGVRERLKIDVDDIRAHNPSVIYVRGTGQGERGPDADKGSYDSLAFWA
                     RAGIAIGAQRPEYDLTPIPPAPGFGDSIGAMTIAGGVMGALYQRERTGHAAVVDVSLL
                     GTGLWAMGQAMALSLSLGVPWQAPPADRIGANPLSRNYD"
     gene            complement(949..2463)
                     /locus_tag="PLP95_06880"
     CDS             complement(949..2463)
                     /locus_tag="PLP95_06880"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HPB45561.1"
                     /translation="MHLELVLKMAAAMSGDRVAFGSLEGGTTMADFAERAGRVGARFA
                     ASAGAERVAMVDLNSEAVPLTLFGSVLAAKPFVPINYRLTDDQLRALVARTAPALVVV
                     GEGVAQRIGEIEGIEFLERSELLAIAAGDGEGGGERLDDALDTDPSAIAVLLYTSGTT
                     GDPKAAVLRHLNLGAYVVTTVELGGSSPEEAALVSVPPYHIAGISAAVTSVYGGRRVV
                     YLESFDPVQWVATARREAITHAMVVPTMLGRILDVMEAEGLTLDSLRTLSYGGGPMPA
                     AVIERALSLLPGVDFVNAYGLTETSSTIALLGPDDHRVAFASDDPQVRARLNSVGRPL
                     ASVEVSIRDEDGNEVPVGHTGEVWVRGEQVSGEYLGRDGGGDGWFHTRDAGHLDTEGF
                     LFVEGRLDDVIVRGGENLSPGEIEATLLEHDAVAEAAVVGVPDVEWGEKVVAAVVAVP
                     GAEVDAEELREFVRGRLRSTKTPERISVVEELPFNETGKLLRRTLREQLADPQG"
     gene            complement(2476..2787)
                     /locus_tag="PLP95_06885"
     CDS             complement(2476..2787)
                     /locus_tag="PLP95_06885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB45562.1"
                     /translation="MGIEVKPGDRLRSTVCATAVVVVKAPAGEIDLRCGGAPMVPLAD
                     AGEPSGSPAGGLNNGTVLGKRYTDSEGVEVLCSAGGLGTLSLGEVPLELQGAKPLPSS
                     D"
     gene            complement(2855..>3765)
                     /locus_tag="PLP95_06890"
     CDS             complement(2855..>3765)
                     /locus_tag="PLP95_06890"
                     /inference="COORDINATES: protein motif:HMM:NF012296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HPB45563.1"
                     /translation="TPRRMAAIEPEVRAFCARSLDPLVGADGLDVIADLGAQVPMRTI
                     GMLLGIPESDQVALRDQIDGGLKLAEGEMPTISEGFGAMSMTEQFEEYIDYRYKNPSD
                     DVMTDLITAEYEDLDGVYRRLTREEALNYINLVAAAGNETTNRLIGWSTRLLSDHPGE
                     REIVVSDPSIVPNMIEEVLRYESPSPVQARYVTRDVEYHGTTVPAGSAMLLLNGSGNR
                     DERVFDDPDRFDVRRDVSHHLAFGHGIHFCLGAALARLEGRVAMQELLARFPNFHVDD
                     SVAKRGRTSTVRGWESLPIVIDRTAS"
BASE COUNT          620 a         1343 c         1195 g          607 t
ORIGIN      
        1 tgtcgtagtt gcgcgacagc gggttggccc cgatgcgatc ggcgggcgga gcctgccacg
       61 gcacaccgag cgacagcgac agcgccatgg cctggcccat cgcccacagg ccggtgccga
      121 gcagcgacac gtcgaccacc gctgcgtggc cggtgcgctc acgctgatac agcgccccca
      181 tgaccccgcc ggcgatggtc atggcgccga tcgagtctcc gaatcccggc gccgggggga
      241 tcggggtgag gtcgtattcg ggtcgttgtg cgccgatggc gatgccggcg cgcgcccaga
      301 aggcgaggga atcgtatgac cccttgtcgg cgtcgggccc acgctcaccc tgaccggtgc
      361 cccgcacgta gatgaccgac gggttgtgcg cgcggatgtc gtcgacgtcg atcttcagcc
      421 gctcgcgaac tccgggcaac ttgttggtga tgaacacgtc cgcggtggcc gccaaccgat
      481 agagcacctc aaggccctcc ggttgagtga ggtcgaggcc gaggctcttc ttgccgcggt
      541 tggagtgctc caacaacgcg tgcacctcgc cgccgagttc ggccactccg gtcgacgcca
      601 ggccgcgcat ggcatcaccg cgggtgacgt gttcgatctt gatgacctcc gcaccccaat
      661 ccgccagcag cgccgaggcc gccggaacga aggtgtgctc ggcaacctcg agcacgcgga
      721 cgccctcgag cacccgtgcg ccgtgcgctg cgccatcgtg cgcctgtggc atgtcgaaat
      781 ccccctgtga gttcccctgc gttgggcccc gatgccaacg cgtcaacgcc atatccactg
      841 tagcggaaac gccattaccg gtttttgcaa actttactct cagccgagca tttcaaccct
      901 gccgtttcaa ccctgccgtt tcaaccctga ccgttcaccc ctgccgtttc agccctgagg
      961 atcggcgagt tgttcacgca gcgtgcgcct cagcagcttt ccggtctcgt tgaagggcag
     1021 ctcctccacc accgagatgc gttcgggggt cttggtcgaa cgcagtcggc cccgtacaaa
     1081 ctcgcgcaac tcctcggcat cgacctcggc cccgggcacg gccaccaccg cggccacgac
     1141 cttttcgccc cattcgacat cgggcacgcc gacgaccgcg gcctcggcga ccgcgtcgtg
     1201 ttcgagcaag gtagcctcga tctcgcccgg tgaaaggttc tcgccgcctc gaacgatgac
     1261 gtcgtcgagt cgaccttcga cgaacaggaa cccttcggtg tcgaggtggc cggcgtcgcg
     1321 agtgtgaaac cacccatcgc cgccgccatc gcggcccagg tactcccccg agacctgttc
     1381 gcctcgcacc cagacctcgc cggtgtgccc caccggcacc tcgttgccgt cctcgtcgcg
     1441 tatcgacacc tcgaccgagg caagcggccg ccccaccgag ttcagacggg cgcgcacctg
     1501 cgggtcgtct gaggcgaacg cgacccgatg atcatcgggg ccgagcagcg cgatggtgct
     1561 cgaggtttcg gtcaacccgt acgcattcac aaagtccacc ccgggcaaca gcgacagcgc
     1621 ccgttcgatc accgccgcgg gcatcggccc gccgccatag gagagggtgc gcagcgaatc
     1681 gagtgtcagc ccctcggcct ccatgacatc gaggatgcgg ccgagcatgg tcggcaccac
     1741 catcgcatgg gtgatcgcct cccggcgcgc cgtggcgacc cactgcacgg gatcgaacga
     1801 ctcgagatac accacacgtc ggcccccgta caccgaggtc accgcggcgg aaatccctgc
     1861 gatgtgatac ggcggaacgc tcaccagcgc cgcctcctcg gggctcgagc cgccgagttc
     1921 caccgtcgtc acaacgtatg caccgaggtt gaggtgtcgc agcaccgcgg ccttgggatc
     1981 gcccgtggtt ccgctcgtgt acaacaacac ggcgatcgca gacggatcgg tgtcgagggc
     2041 atcgtcgagg cgctctcctc caccctcgcc gtcaccggcg gcgatggcca acaactcgct
     2101 gcgctccagg aactcgatcc cctcgatctc gccgatccgt tgggccaccc cctcgcccac
     2161 caccaccagc gctggcgcgg tacgggccac gagggcccgc agttggtcgt cggtgaggcg
     2221 gtagttgatc ggcacgaacg gcttggccgc cagcaccgag ccgaacaggg tgagcggaac
     2281 cgcctcggag ttcaggtcga ccatggccac gcgttcggcc ccggccgacg cggcgaaccg
     2341 cgccccgacc cgcccggccc gctcggcgaa gtcggccatc gtggtgccgc cctccagcga
     2401 gccgaacgcg acgcggtccc ccgacatcgc ggcggccatt ttcaacacga gttcgaggtg
     2461 catcgggcct gccgttcagt ccgaggacgg caggggtttg gccccctgga gttcgagcgg
     2521 aacctcgccg agcgacaagg tgccgagccc gcctgccgag cacagcactt cgacaccctc
     2581 ggaatcggtg tagcgcttgc cgagcaccgt gccgttgttg agcccaccag cggggctacc
     2641 gctcggttcg ccggcgtcgg cgagtggcac catcggtgcc ccaccgcagc gaaggtcgat
     2701 ctcacctgcg ggggccttga ccacgacgac cgccgtcgca cagaccgtcg agcgcagccg
     2761 atcccctggc ttcacttcga tccccatttt gtgtcctttc gtaaacgggc cgcccacgac
     2821 gacccctcgc agatgcttac tcgacgatcg cgcctcacga ggccgtccga tcgatgacga
     2881 tgggcagcga ctcccagccg cgaaccgtcg aggtgcgacc ccgtttggcg accgagtcgt
     2941 cgacatggaa gttcgggaac cgggccagca gttcctgcat cgccacgcgt ccttcgagac
     3001 gggcgagcgc ggcaccgagg cagaaatgga tgccgtgccc gaacgccagg tgatgggaca
     3061 cgtcgcgacg cacgtcgaag cggtcggggt cgtcgaaaac ccgctcgtcg cggttgcccg
     3121 aaccgttgag cagcagcatc gcggagcctg cgggaacggt cgtgccgtga tactcgacgt
     3181 cgcgggtgac gtagcgggcc tgcaccggcg acggtgactc gtagcgcagc acctcctcga
     3241 tcatgttggg cacgatcgag gggtccgaca cgacgatctc acgctcaccc gggtggtcgg
     3301 acaacaaccg cgtcgaccac ccgatgaggc gattggtcgt ctcgttcccg gctgcggcga
     3361 cgaggttgat gtagttgagc gcctcttcgc gggtcaggcg gcgataaacg ccgtcgaggt
     3421 cctcgtattc ggcggtgatg aggtcggtca tcacatcgtc ggaggggttc ttgtaccggt
     3481 agtcgatgta ctcctcgaac tgctcggtca tcgacatcgc tccgaagcct tcggagatgg
     3541 tcggcatctc cccctcggcg agcttcaggc cgccgtcgat ctgatcgcgc aacgccacct
     3601 ggtcgctttc gggaatgccg agcaacatcc cgatcgtgcg catcggcacc tgcgccccga
     3661 gatcggcgat cacatcgagt ccgtcggcac cgacgagcgg gtcgaggctg cgagcgcaga
     3721 acgcccgcac ctccggctcg atcgcggcca tgcgacgcgg cgtga
//
LOCUS       DAOOMA010000217         3762 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_29552_length_3762_cov_4.005665, whole genome shotgun sequence.
ACCESSION   DAOOMA010000217 DAOOMA010000000
VERSION     DAOOMA010000217.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 3762)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3762)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3762
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29552_length_3762_cov_4.005665"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..818
                     /locus_tag="PLP95_06895"
     CDS             <1..818
                     /locus_tag="PLP95_06895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB45564.1"
                     /translation="FGRPAFLIGLALTYAALLPYLDGDEPTDELSMIAPAALLGMVGI
                     SVSAHRVWASDRCAEAAGPTPVGQVSRTVGHLVDCLVPALAGLGFVLVTLVRAQSVST
                     PRGGYAEMMSDGWVAAVWLALGAVSCLGGPVLGVAVARRTKRAAAPVLATVGLVLTVI
                     VFQGLFEPLRRVRVLLPWTYWGGPFGIEGDPQRSIVLSGSPQWWLVYLLALCAIGAVL
                     AVRADPEARTARSRSALFVLVGMAAVTVLLAMFTGTPETIVSPVPGDPTDSGG"
     gene            815..1468
                     /locus_tag="PLP95_06900"
     CDS             815..1468
                     /locus_tag="PLP95_06900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB45565.1"
                     /translation="MSIVSGLRGGALRYLSAATRWPLLTGAVCLVAGGVALVARWPDA
                     LWPVHGTVLGVVVGAAAVAVDERCALVVDVSPRPLWWRTAARAIGPTVLVAVWATVHW
                     VFRASLPKHLWVLILQGAVAAGIGFGLATGARASGRSEPGTVLAATAIPLIAGVALAR
                     PFETDLPLFPVWPHENWDRALTIWMALGVGAVLVGVGALWSDARQRRSLGYPHRSDR"
     gene            1543..1968
                     /locus_tag="PLP95_06905"
     CDS             1543..1968
                     /locus_tag="PLP95_06905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB45566.1"
                     /translation="MLSTRIRHISQAALIATLCVGSLAACSNDTENAADTAPTTTAAD
                     ATAPADDLEAFCAVATELVGVTPTSEQLAAYAEAAPVGIAEAVGAFVAAFEAAEGDLD
                     AVFADPDASAAADEIAVFESDECGITQPGPPPGAPGNGG"
     gene            complement(2033..2545)
                     /locus_tag="PLP95_06910"
     CDS             complement(2033..2545)
                     /locus_tag="PLP95_06910"
                     /inference="COORDINATES: protein motif:HMM:NF024966.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HPB45567.1"
                     /translation="MGDIIELTHDDLVNIELLKRLKYRYLRCLDLKMWDDLAECFTSD
                     ATASYGGGAYQFDGRDAIMEFLRTSMGSESMLTSHRCHHPEIDLVDADSATGTWALDD
                     VVILSNFGLTIQGSAFYEDRYVRVDGRWLIAHTGYRRIYEEMYPRESVDGLRLTASWW
                     GTDGRSELQA"
     gene            complement(2621..3040)
                     /locus_tag="PLP95_06915"
     CDS             complement(2621..3040)
                     /locus_tag="PLP95_06915"
                     /inference="COORDINATES: protein motif:HMM:NF022072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HPB45568.1"
                     /translation="MFEHSIEIAASPAAVFAVWTDVTRWPEWTRSVTSIEMVVDLPLQ
                     IGAKVRIRQPRLGTTTWTISEFEPDQAFTWVSSAPGLAGQATHRVQQTPTGTRATMSV
                     DHTGFLAPIARALTAGITRRYLTYEAEGLKARAESGE"
     gene            3124..>3762
                     /locus_tag="PLP95_06920"
     CDS             3124..>3762
                     /locus_tag="PLP95_06920"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HPB45569.1"
                     /translation="MSITPRTTTRRTQRAVLAFLLAIAPLSACRSDPGDTSADNQDAD
                     STIVESVTSAPTGNAGADSDTNDADSPDEVIFPGADWELSSPETEGFDAEALEHALDA
                     ALIPTANTQGVVVVRHGKIVTERYADGAASDSWAASWSVAKSFASALVGIAVDEGNIS
                     SVDTPMTTWFPDWEARGLGDITLRHVLQMSTGLQWNEDYNPSNGPSDIIALVT"
BASE COUNT          581 a         1322 c         1228 g          631 t
ORIGIN      
        1 acttcggtcg ccccgccttc ctcatcgggt tggctctcac ctacgccgcg ctcctcccct
       61 acctcgacgg cgacgagccg accgacgagc tgtcgatgat cgccccggcc gccctgctcg
      121 ggatggtcgg catcagcgtg agcgcgcatc gggtgtgggc gtcggaccgc tgcgcagagg
      181 cagctggccc gacaccggtc ggtcaggtgt cgcgaaccgt cgggcacctg gtcgactgcc
      241 tcgtgccagc cctggccggc ctgggcttcg tgctggtgac gctggtgcgg gcgcagtcgg
      301 tgtcgactcc tcgtggcggg tacgcggaga tgatgtcgga cgggtgggta gctgcggtgt
      361 ggctggccct gggagcggtg agttgcctgg gcgggccggt cctcggagtg gccgtggccc
      421 ggaggacaaa acgtgccgcc gcacccgtcc tcgcgacagt cggtctcgtg ttgaccgtga
      481 tcgtgttcca gggtctgttc gaaccgctcc gccgtgtccg ggtcctactt ccctggacct
      541 actggggtgg ccccttcggc atcgagggcg acccgcagcg ctcgatcgtg ctcagcggct
      601 caccgcaatg gtggctggtc tacctcctcg cgctctgcgc gatcggtgcg gttctcgccg
      661 tgcgcgccga cccggaggcc cgaactgccc ggagtcggtc ggccctcttc gtgttggtcg
      721 ggatggcggc ggtcaccgtc ctgctcgcga tgttcaccgg gacgccggag acgatcgtca
      781 gccccgtgcc cggggaccca accgactcgg gcgggtgagc atcgtgtcag ggctgcgcgg
      841 tggggcactg cgctacctca gcgcagcaac ccgatggccc ctgctgaccg gcgcggtttg
      901 cctcgtggcc ggcggcgtcg ccctcgtggc gcgctggccc gatgctctct ggccggtgca
      961 cggcaccgtt ctgggagtcg tggtcggagc agccgctgtc gccgtggacg agcggtgcgc
     1021 cctcgtggtc gacgtcagcc cccgtccgct ctggtggcga accgcggccc gagccatcgg
     1081 cccgacggtc ctcgtcgcgg tgtgggccac agttcactgg gtgtttcgag cgtccctgcc
     1141 caaacacctc tgggtcctca tcctgcaggg cgcggtcgct gcgggaatcg gattcgggct
     1201 ggccaccggc gcccgggcga gcggccgcag cgagccaggc acggtcttgg ccgcaacggc
     1261 catccccttg atcgcagggg ttgcactcgc tcgccccttc gagaccgacc tcccgctgtt
     1321 ccccgtatgg ccacacgaga actgggaccg tgccttgacc atctggatgg cactgggcgt
     1381 aggcgccgtg ttggtcgggg tcggagcctt gtggagcgac gcccgccagc gtcgatccct
     1441 cggctacccg catcgcagcg atcggtgatc ccgaagcgat caccgaagcg acagagcagc
     1501 gggacccgaa gccgtcgggc ccgcccgcat ttggaggaac ccatgttgtc cacccgtatc
     1561 cgccacatct cacaggcggc attgatcgcc acgctgtgcg tcggctcgct cgccgcgtgc
     1621 agcaacgaca ccgaaaacgc tgccgacaca gcgccgacga ccacggccgc cgacgcgacc
     1681 gctcccgccg acgacctcga agccttctgc gccgtcgcca ccgagctggt cggagtcact
     1741 ccgaccagcg agcaactcgc cgcgtacgca gaggcagcgc ccgttggtat cgctgaggcg
     1801 gtcggcgcgt tcgtcgcagc gttcgaggct gccgagggtg accttgacgc ggtgttcgcc
     1861 gacccggacg cttcggccgc cgccgacgag atcgcggtgt tcgaatccga cgagtgtggc
     1921 atcacccagc ccggcccgcc tccgggcgcc cccggcaacg gcggctagcc aaccgagcgc
     1981 ctcggaacgc gatcggtcaa agcagcccga ttagtggagg cgttgcaggc gactacgcct
     2041 gcaactcgga gcgtccgtcg gtcccccacc acgacgccgt caatcggagc ccgtccaccg
     2101 attcgcgtgg atacatctct tcgtagatac gcctgtagcc ggtgtgagcg atcagccatc
     2161 gaccgtcgac ccgaacgtag cggtcctcgt agaacgctga accctggatc gtcaggccga
     2221 agttcgacag gatcaccacg tcgtcaagcg cccacgttcc ggtcgccgag tccgcgtcga
     2281 ccaggtcgat ctcggggtga tggcaccgat ggctcgtgag catcgactcg ctccccatcg
     2341 acgtccgaag aaactccatg atcgcatcac gcccgtcgaa ctggtacgcc ccgcccccgt
     2401 agctcgcggt ggcgtcgctg gtgaaacact cggcgaggtc gtcccacatc ttcaggtcga
     2461 gacaccgtag ataccggtat ttcaagcgct tgagcagctc gatgttcacc aggtcgtcgt
     2521 gtgtcagttc gatgatgtca cccaccgccg cagcctacct acacggcagt cgccgaacgc
     2581 ccctgcaaca ccgatcggtt cgatgtcggt gacggcgcgg tcactcaccg gactcggcgc
     2641 gggccttcaa cccctcggcc tcataggtca ggtaccggcg cgtgataccg gcggtgagcg
     2701 ctcgagcgat gggtgccagg aacccggtgt gatcgacgct catcgtggcc cgagtgcccg
     2761 tcggggtctg ttggacgcga tgggtggcct ggccggccag cccgggagcc gagctgaccc
     2821 acgtgaacgc ctgatcgggt tcgaattcgc tgatcgtcca agtggtcgtg ccgagtcgtg
     2881 gctggcgaat ccggaccttc gcgccaatct gcaatggcag gtcgacgacc atctcaatcg
     2941 aggtcaccga acgggtccat tccggccaac gcgtcacgtc ggtccagaca gcgaacacgg
     3001 ccgcgggcga ggcggcgatt tcaatcgaat gctcaaacac caatcgcaac gataggtcga
     3061 cgatgcgaag tcgacgccgt cgttttccgc gcccggagaa tcgggtcctg ccaaactcct
     3121 gccatgtcga tcacaccccg gaccacgacg cgccggaccc aacgcgctgt gcttgcgttc
     3181 ctgctcgcca tcgccccgct ctccgcgtgc cgcagcgacc cgggcgatac ctcagccgac
     3241 aaccaggatg ccgactcgac gatcgtcgag tcggtgacgt cggcgcccac ggggaacgcc
     3301 ggtgcggatt ccgacaccaa cgatgccgat tcgccggacg aggtcatttt tccgggcgcc
     3361 gactgggaac tctcctcccc cgagacggaa gggttcgacg ccgaggcgct cgaacacgcg
     3421 ctcgacgcag cgctcatccc aaccgccaac acccaggggg tcgtggtcgt tcgccacggc
     3481 aagatcgtca ccgaacgcta cgccgacggc gccgcatccg attcgtgggc agcgagctgg
     3541 tcggtggcca aaagcttcgc aagtgcgctc gtgggcatcg ccgtcgacga gggcaacatc
     3601 tcgagcgtcg atacccccat gacgacctgg ttcccagact gggaggcacg cgggctcggc
     3661 gacatcaccc ttcgtcatgt cctgcaaatg tcgaccggct tgcagtggaa cgaggattac
     3721 aaccccagca acggcccctc cgacatcatc gccttggtga cc
//
LOCUS       DAOOMA010000218         3730 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_29882_length_3730_cov_3.318912, whole genome shotgun sequence.
ACCESSION   DAOOMA010000218 DAOOMA010000000
VERSION     DAOOMA010000218.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 3730)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3730)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3730
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29882_length_3730_cov_3.318912"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..543
                     /locus_tag="PLP95_06925"
     CDS             <1..543
                     /locus_tag="PLP95_06925"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HPB45570.1"
                     /translation="AWINHGERELYSSPWVTLRLADVEWPDGTRFDHHLIRCQPAAGC
                     IVIDPERGVLLLWRHRFITDTWGWEIPAGRVDEGESIVDAARRETLEETGWEPEGLTP
                     LVDFYPSNGLSDQHFHVFVATGATHRGEPSDPNEADRVEWVSVEDLREALRRGEMNEG
                     LSVTACCYALAFGAFGDLDA"
     gene            complement(600..1763)
                     /locus_tag="PLP95_06930"
     CDS             complement(600..1763)
                     /locus_tag="PLP95_06930"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020376938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HPB45571.1"
                     /translation="MTTTFESLGVSAPLSAALTERGITSPFAIQALTIEDALAGHDVC
                     GKAKTGSGKTLAFGVPTLQVLPKAKPNRPTGIVLVPTRELATQVRDELAPLGAAIDRT
                     VVAIYGGDPIDKQINKLNRGVDLMVCTPGRAIDLIEREALSVSDITNVVVDEADRMAD
                     MGFLPQVEWILRQVDGEHQTLLFSATLDGVVDGLVQRYQHDPKLHEVESKTVTVDQMH
                     HRFLKVHELDRVKVLASIIEGSNKTMVFTATKRDADRIVVKLQGEGVNAAAIHGDLRQ
                     QLRERTLADFASGKIKALVATDVAARGIHVDDVDVVIHLEPATDHKTYLHRSGRTARA
                     GTSGVVVTLALWNQELEVKRLQKRIGLNIPIVEVFSNNPLLRDLVAWDPEEGA"
     gene            1926..2114
                     /locus_tag="PLP95_06935"
     CDS             1926..2114
                     /locus_tag="PLP95_06935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB45572.1"
                     /translation="MYNTPPAWATAEAIEELVSAPAQRNEHTDLSLAQHAAQYLAARK
                     VKPYQHVIVDESQDLHEA"
     gene            2136..2753
                     /locus_tag="PLP95_06940"
     CDS             2136..2753
                     /locus_tag="PLP95_06940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB45573.1"
                     /translation="MPEQPNDMFIVGDQHQRIYDRRSALSKVGIRITRRSKRLRINYR
                     TPHEIMAWSLMLLDGADGGVDAGGGVEVGAGAAVGEGDDPLNFAGYHSFMRGPTPTIY
                     EARSRADQRKALVKVVTQWIDDGVQSAEIGVAARKTAEVNALYNALDAAGVPVEKLAK
                     CKPRSNGVRVATMHRTKRLEFRCVAIDDVSLGELERNSALAAPPS"
BASE COUNT          642 a         1180 c         1258 g          650 t
ORIGIN      
        1 gcctggatca atcacggcga acgcgagctg tattcgagtc cgtgggtcac cctcaggctc
       61 gcggatgtcg agtggcccga cggcacccgt ttcgaccacc acctgatccg ttgccagccc
      121 gccgccggat gcatcgtgat cgaccccgaa cgtggggtgc tgttgttgtg gcggcatcga
      181 ttcatcaccg acacctgggg ttgggagatc ccggccgggc gggtcgatga gggcgagtcg
      241 atcgtggatg ccgcacgccg agagaccctc gaggagaccg ggtgggagcc cgagggcctc
      301 accccgctgg tcgacttcta tccgtcgaac gggttgagcg atcagcactt ccacgtcttc
      361 gtcgcgaccg gcgccaccca ccggggcgag ccgagcgacc ccaacgaggc cgaccgggtc
      421 gagtgggttt cggtcgagga cctgcgcgag gcgctgcgtc gcggcgagat gaacgagggc
      481 ctgtcggtga cggcgtgttg ctacgcgctc gcgttcggag cattcggcga cctcgacgcc
      541 tgagccgcaa gcgcccgcgc ccgagccagc accggcgcgc cagcgcgtac cggccccgat
      601 caggcccctt cttcggggtc ccacgccacg aggtcgcgca gcaacgggtt gttcgagaac
      661 acctcgacga tggggatgtt caacccgatg cgcttctgca gccgcttcac ctcgagttcc
      721 tggttccaca acgcgagggt gaccaccacg ccggaggtgc cggcgcgagc ggtgcgaccc
      781 gatcggtgca gataggtctt gtgatcggtg gcgggttcga ggtggatcac cacgtccaca
      841 tcgtcgacgt gaatgcctcg cgccgccaca tcggttgcga cgagggcctt gatcttgccg
      901 gaggcgaaat cggcgagcgt gcgttcgcgc agttgctggc gcaggtcgcc gtggatcgcc
      961 gcggcgttca ctccctcgcc ctgcagtttc accacgatgc gatcggcgtc gcgtttggtg
     1021 gcggtgaaca ccatcgtctt gttcgaacct tcgatgatgg aggcgagcac cttcacccgg
     1081 tcgagttcgt gcaccttcag gaatcggtgg tgcatctggt caaccgtgac ggtcttcgat
     1141 tcgacctcgt gcagcttcgg atcgtgctgg tagcgctgca ccagcccatc gacgacgccg
     1201 tcgagggtcg ccgagaacag cagggtttgg tgttcgccgt cgacctgtcg aaggatccac
     1261 tcgacctgtg gcaggaaccc catgtcggcc atgcggtcgg cctcgtcgac gacgacgttg
     1321 gtgatgtcgc tcaccgacaa cgcctcgcgc tcgatgaggt cgatggcccg accgggcgtg
     1381 cacaccatga gatcgacgcc gcggttgagc ttgttgatct gcttgtcgat ggggtcgccg
     1441 ccgtagatcg ccacgacggt gcggtcgatg gcggcgccga gcggggcgag ttcatcgcgc
     1501 acctgggtgg cgagctctcg ggtcggcacc agaacgatgc cggtggggcg gttgggtttc
     1561 gccttcggca gcacctgcaa ggtggggact ccgaaggcca gcgtcttgcc ggatcctgtc
     1621 tttgcttttc cgcacacgtc atgaccggcg agtgcgtctt cgatcgtgag cgcctgaatg
     1681 gcgaatggtg aagtgattcc acgctcggtg agggctgcgg acaggggtgc cgacacgccg
     1741 agcgattcaa acgttgttgt catacatatt cctcggcgcc aagcctaccg aacccccggc
     1801 gggtgtcggc catggtcgga ttggcgctcg ctcgctggcg cgctcgtcgg cgcgctcgct
     1861 ggccgggttc gcgggccagg tttgaggcga gtgttgtaca tcaggggcgc gatatcgcgc
     1921 ccctgatgta caacaccccg cctgcgtggg cgacggccga ggccatcgag gagctcgtca
     1981 gtgccccggc gcaacgcaac gagcacacgg atttgtcgct cgcacagcat gcagcgcaat
     2041 acctggcggc gcggaaggtg aagccgtacc agcacgtgat cgtcgacgag tcccaggatc
     2101 tccacgaggc gtaatggcga ctgctgcggg cggcggtgcc ggaacagccc aacgacatgt
     2161 tcatcgtcgg cgaccagcat cagcgcatct atgaccgcag gtcggcgctg tcgaaggtgg
     2221 ggatccgcat caccaggcgg tcgaagcggc tgcgcatcaa ctaccgcacg ccccacgaga
     2281 tcatggcgtg gtcgctgatg ttgcttgacg gtgccgatgg tggtgttgat gctggtggtg
     2341 gcgttgaggt gggtgctggc gcggcggtcg gtgagggcga cgatccgctg aactttgccg
     2401 ggtatcactc gttcatgcgg ggaccgacgc cgaccatcta tgaggcgagg tcacgggccg
     2461 accagcgaaa ggcgttggtc aaggtcgtca cccagtggat cgacgacggc gtgcaatcgg
     2521 ccgaaatcgg ggtcgccgca cgcaagaccg ctgaggtgaa cgcgctctac aacgccctcg
     2581 acgccgcggg tgtccccgtc gagaaactgg cgaagtgcaa gcctcgctcc aacggcgtgc
     2641 gggtcgccac gatgcaccgg acgaaacggc tcgagttccg ctgcgtcgcc atcgacgacg
     2701 tcagcctggg tgagttggaa aggaactccg cgcttgcggc accaccatcc tgaacggatg
     2761 tgtgccgcct tgaaggttca ggggcagatc caacgcggcg atgacaacac cctcgaccga
     2821 ccacggctcg ggccacgcgg tgtaggagac gccacaagat tcggcgatct atcgagtgag
     2881 gggagcctca tcggtcaggc acggccgacg cgcgtcgctc acaagctgca ggtcaaggtg
     2941 ccccatgagc aacgccgcca tgctcagcct gaaggttgag gagccgatcc tgcccgaaac
     3001 gtgatcggcc aaccgactcg ctaccactcg ggtactcgcc gccgaacttg ggctgatccc
     3061 gacgtacaag ggcagcgctt cggatttgag cggggtcggg tggagggtcc gcgtgtgtgg
     3121 gtcaggggct cgaatcgacg atcatggtcg cgcctcgacg cgcaccctca acacacaact
     3181 cgcgggccgc cgcgttgacc gctgagacct cgcgagttgc cgcccccgca accgcgacga
     3241 aaaggttgtc aacccttcgg ggaactgaat gatctcaccg atcgtcgatc ggtgagtgtc
     3301 gagcttggcc atgtcccgca gcgactgggc cgatgtggca ccaagcaacc ggtgaacctc
     3361 cggcttgcgg aagtcgccgc cgaccaccag ggtcggcaca ccgacctcag cgagggactt
     3421 cgaaacgagc gcagaggtgg tcgacttgcc ctcccccggt atcgtcgagg tgacatcagg
     3481 atgagcgatg tcggctacga cccatcccta agcttcacgg aaccgccacc gaatccgagt
     3541 cgatgcgcct cacgctcgac gactacaacg gtcgacgcag tggggtcagc gacctactca
     3601 tcaccctgct cgggtttgag tcccgtcgag cgatgagtcg aaggtgagga ggctgtcgag
     3661 ttcgtccacc ggcgccgtca cccgatgtcg aggagcactg cgatcgtctc acgcacctgg
     3721 gccaacacct
//
LOCUS       DAOOMA010000219         3727 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_29907_length_3727_cov_3.912854, whole genome shotgun sequence.
ACCESSION   DAOOMA010000219 DAOOMA010000000
VERSION     DAOOMA010000219.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 3727)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3727)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3727
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29907_length_3727_cov_3.912854"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..371
                     /locus_tag="PLP95_06945"
     CDS             <1..371
                     /locus_tag="PLP95_06945"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB45574.1"
                     /translation="TKVFTFGLSSALAGLAGSLFMLQLATNKANLASGSFGLQLSLYL
                     LVAVVIGGSQSIAGPLIGTFIIVFFRDVIQPELPKRIHEIMPLLFGISLITTMFAGPG
                     GIVGQFRHGVHKIHARRHKS"
     gene            416..1786
                     /locus_tag="PLP95_06950"
     CDS             416..1786
                     /locus_tag="PLP95_06950"
                     /inference="COORDINATES: protein motif:HMM:NF024850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HPB45575.1"
                     /translation="MMKTRAVRAAALATTLSLVLAGCGRDDDEAANDSPTTVADGESG
                     GENTESAGFIDPATDCAADYNATQGINGDTITIGNVRPETGNYSIYGTVAKGLEAFVQ
                     HANSQGGITAGDGKQYKLQLETADDAYDPALTPGVVQTLVEQKGIFAMVGQIGTATNL
                     AVRQYMNDNCVPSVALATGARDWGEAAKYPWYIGGLPPYATEAVAFIDYLKTEKPDAT
                     IALLYQDDDYGKTYQQAIAQAIEGTDITVVSEKPYNPLNETSPESKVAELATSNADVF
                     FVGLGGTPCPVSLTKVPDTWTPMTFVSVTCSAKTAMSLLGDKEASMNVFAAQPALDPA
                     NPEHQADPAMQEVFAAGRAIGLDEATLEGGIFAVGWNFGALFARGLENAETVTRHDVM
                     NALWSLEDEEIGIALPGAVANTNGAEDPWPFEDMRIVHRVDGLWTPVTEVISYDGKGN
                     EFGGIG"
     gene            1912..2229
                     /locus_tag="PLP95_06955"
     CDS             1912..2229
                     /locus_tag="PLP95_06955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB45576.1"
                     /translation="MSAKRFALGSVALICLLLAGWLATTDVHVDNATCGTALLRRDPL
                     ASLVVTGDIVEDALTEDWLTSICGRNITRQRILTAIPLAVGVAAIITGSRERALDEAV
                     NAR"
     gene            complement(2242..2838)
                     /locus_tag="PLP95_06960"
     CDS             complement(2242..2838)
                     /locus_tag="PLP95_06960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB45577.1"
                     /translation="MKPVCALVASGLMIAAVTTGCAGDNAESDGPATTDLNPTPTTMV
                     PQAGLIAQPVESGGVRVEVLEIAPTYHSPKDRAQIIAEVRSENLSGVSQRNPNVSLVC
                     ADRVAVGEWAEGSTWEPNGILSVNAVLSGNVIIGFPQAGPNPEYPSVTCEDATLRVQI
                     VSLSAETVTIDFPVAADIIETALRSPNSAEMPLPYAEG"
     gene            complement(2838..>3727)
                     /gene="rlmB"
                     /locus_tag="PLP95_06965"
     CDS             complement(2838..>3727)
                     /gene="rlmB"
                     /locus_tag="PLP95_06965"
                     /inference="COORDINATES: protein motif:HMM:TIGR00186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HPB45578.1"
                     /translation="APRSGSAGAGPAFGGSGAQRGDRSRTNRKAPEQRGLGGTQVEGR
                     HAVRELLLAGTRKAKEILIAEGMDRADILDDIVELAQDLRVPVREVSKKKLDGVSRTE
                     ASQGVVAVADPIKERPLEELCETQNGMAPFLVAVDGVTDPGNLGAIIRTAECAGVSGM
                     VLPKHRAVHVTPTVTKAAAGAVEHVPMALVGGLPRAIEEMQRAGVWVLGLDMGGDVSI
                     HEVALGAEPVCLVMGAEGAGLSRLVRERCDQVVSIPLAGQLSSLNVSTAAALAIYEVV
                     RRRGSEARAEGPAGAAGGR"
BASE COUNT          647 a         1329 c         1123 g          628 t
ORIGIN      
        1 ccaccaaggt gttcaccttc ggtctcagtt cggcactcgc cggactcgcg ggttcgctgt
       61 tcatgttgca gctcgccacg aacaaggcca acctcgcctc gggctcgttc ggccttcagc
      121 tttcgctgta cctgctcgtc gccgtcgtga tcggcggcag ccagagcatc gccggcccgc
      181 taatcggcac cttcatcatc gtgttcttcc gagatgtgat tcagcccgag ttgccgaaac
      241 gcatccacga gatcatgccg ttgctgttcg gcatctcact gatcaccacc atgttcgccg
      301 gccccggggg tatcgtcgga caattccgtc acggcgtgca caagatccac gcacgacggc
      361 acaagagctg aaccgcgaca ccgtcgcacc agcaacaacc gttagggagg aaaccatgat
      421 gaagacgcgt gctgttcgtg cagctgcgct cgccaccacc ttgtcgctcg tgctcgccgg
      481 atgcggtcga gacgacgatg aggcggccaa cgacagcccc accaccgttg ccgatgggga
      541 gtcgggcggc gagaacaccg agtccgctgg tttcatcgac ccagcgaccg actgtgccgc
      601 cgactacaac gccacccagg gcatcaacgg cgacaccatc accatcggca acgtccgtcc
      661 ggaaaccggc aactacagca tctacggaac cgtggccaag ggcctcgaag cgttcgtcca
      721 acacgccaac agccaggggg gcatcaccgc cggcgacggc aagcagtaca agctgcagct
      781 cgaaaccgcc gatgacgcct acgacccggc cctgaccccc ggcgtcgtcc agacgctggt
      841 ggaacaaaag ggcatcttcg cgatggtcgg ccagatcggc accgcaacca acctcgccgt
      901 tcgtcagtac atgaacgaca actgcgtccc gtcggttgcc ctcgccaccg gcgcccgcga
      961 ctggggggag gcggccaagt acccgtggta catcggcggg ttgccgccct atgcgaccga
     1021 agcggtggca ttcatcgact acctcaagac cgagaagccc gatgccacca tcgccctgct
     1081 ctaccaggac gacgactacg gcaagacgta tcagcaggcg atcgctcaag cgatcgaagg
     1141 caccgatatc accgtcgtgt ccgaaaagcc gtacaacccg ctcaacgaaa cctcaccgga
     1201 gtcaaaggtt gccgagctcg ccacgtcgaa cgcggatgtg ttcttcgtcg gcctcggcgg
     1261 caccccatgc ccggtctcgt tgacgaaggt gcccgacacc tggactccga tgacgttcgt
     1321 gtcggtgacc tgttcggcga agaccgccat gtcgctgctc ggcgacaagg aagcctcgat
     1381 gaacgtcttc gctgctcaac cggcgctcga cccggccaac cccgaacatc aggcagaccc
     1441 ggcgatgcag gaggtgttcg ccgccggccg tgccatcggt ctcgacgaag ccacccttga
     1501 gggcggcatc ttcgccgttg gctggaactt cggtgcgttg ttcgcacgcg gcctcgaaaa
     1561 cgccgagacc gtcacccgcc acgacgtcat gaatgccctc tggagccttg aagacgagga
     1621 gatcggcatc gccctgcccg gtgctgtggc caataccaac ggcgccgaag atccgtggcc
     1681 cttcgaggac atgcgcatcg ttcaccgcgt cgacggcctc tggacgccgg tcaccgaggt
     1741 catctcctac gacggtaagg ggaacgagtt cggcggcatc ggctgacgcc atgcctgcgg
     1801 gcccagtgcc ctgaaccacc ttttggttaa acgaggggga gggccggatc tgtgatccgg
     1861 tcctccccct cgttgtttcg cgacgccagc gtccggtagc gtgcttcgcc gatgtccgcc
     1921 aaacgtttcg cgctcggcag cgtcgcactc atctgtctgt tgctcgcggg ctggttggcc
     1981 accaccgacg ttcacgtcga taacgcgacc tgcgggaccg ccctgttgcg tcgggacccg
     2041 ctcgcatcgc tcgtcgtcac cggcgatatc gtcgaggatg ctctgaccga ggactggttg
     2101 acctcgatct gtggccgcaa catcacccgc caacgcatcc tcacggcgat tccgctggca
     2161 gtcggtgtcg cagcgatcat caccggtagc cgcgagcgcg ccctcgacga ggccgtcaac
     2221 gcccgttgag ttccgccgcg gtcacccttc ggcgtacggc agcggcatct cggcagagtt
     2281 cggcgagcgc agcgcggttt cgatgatgtc ggcggcgacc gggaagtcga tcgtcaccgt
     2341 ctcggcgctc aacgagacga tctgcacgcg aagggtcgcg tcctcgcagg tcaccgacgg
     2401 gtactcgggg ttgggcccgg cttgagggaa tccgatgatg acgttgccgc tgagcacggc
     2461 gttgaccgac agaattccat tcggttccca ggtcgacccc tccgcccatt ccccaacagc
     2521 gacacggtcg gcgcacacca acgaaacatt cgggttgcgc tgcgagacgc cagagaggtt
     2581 ctcgctgcgc acctcggcaa tgatctgggc gcggtccttc ggcgagtgat aggtgggtgc
     2641 gatctccagc acctcgactc gtacgccgcc ggattccacc ggctgtgcga tcagccccgc
     2701 ttggggaacc atggtcgtcg gcgtcgggtt gagatcggtc gtcgccggac cgtccgactc
     2761 tgcgttgtct cccgcgcacc cggtcgtcac cgcagcgatc atgagccccg aggcaaccag
     2821 ggcgcagacc ggcttcatca ccgtcctccc gccgcaccgg cgggcccctc ggcgcgggcc
     2881 tcactgccgc gccgtcgcac cacctcatag atcgccaggg ccgccgccgt cgacacgttg
     2941 agcgaggaca gttgccctgc aagcggaatt gacaccacct gatcgcagcg ttcccgcacg
     3001 agccgcgata accccgcccc ttcggccccc atcacaagac acaccggttc ggccccgagc
     3061 gccacctcgt ggatcgacac gtcgccgccc atgtcgaggc ccagaaccca cacccctgct
     3121 cgctgcatct cttcgatcgc cctgggaagg ccgccgacca gcgccatcgg cacgtgttcc
     3181 accgctccgg ccgcggcctt ggtcaccgtc ggggtgacgt gcaccgctcg atgcttcgga
     3241 agcaccatgc cgctcacccc ggcacactcg gcggtgcgga taatggcccc gagattgccg
     3301 gggtcggtga ccccgtcgac cgccacgaga aacggggcca ttccattctg ggtctcgcag
     3361 agctcctcga ggggtcgctc cttgatcggg tcggcaacgg cgacgacgcc ctgcgaggcc
     3421 tccgtgcgac tcacgccatc gagttttttc ttcgagacct cccggacggg gacccgcagg
     3481 tcctgggcca gctccacgat gtcgtcgaga atgtcggcgc ggtccatgcc ctcggcgatg
     3541 aggatctcct tggccttacg ggtgccggcc agcagtagtt cccgcacggc gtgtcggccc
     3601 tcgacctggg tgccgcccaa cccgcgctgt tcgggagcct tgcgattcgt ccgcgaccgg
     3661 tcgccgcgct gggcaccaga tccaccaaat gccggcccgg ctccggccga tccgctacgc
     3721 ggcgcac
//
LOCUS       DAOOMA010000220         3725 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_29936_length_3725_cov_3.096730, whole genome shotgun sequence.
ACCESSION   DAOOMA010000220 DAOOMA010000000
VERSION     DAOOMA010000220.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 3725)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3725)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3725
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29936_length_3725_cov_3.096730"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            72..1802
                     /locus_tag="PLP95_06970"
     CDS             72..1802
                     /locus_tag="PLP95_06970"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HPB45579.1"
                     /translation="MRRATDRSGSSLTILAQSAGASLSGDLNSRAEAQRGTALRYLYS
                     GIALAFSATVVISLRQNRNRQADEDLRTSASTDVLTGLANRAMLESWVQDHAEECDEI
                     VLMHIDLDHFKPVNDTYGHAVGDRVLQIAASRLSEVATNYGGLASRIGGDEFVVVLPP
                     QDVDDLERISGRLVDSLIHYEVGGLDLTVGASIGVARGNGSFHDLLIDADLALYQAKR
                     TGRGQASTFKSDAADFVDFIRAELDHGRVRAVYQPQVSLRDGRCIGVEVFARMVDEQG
                     TLLQADGWLSIVEWIGRTDELFEHVITAVAADVDRVKDLACRLWFNVSPADLMRPRGA
                     EWLLGQLARLGLDPEQVGIELTNVEDFNDPRRLSSVLSEIRAAGYGIALDEFGAKDAP
                     LGHLRDLPVSRVKLNSGLVAQVNESMPPSAWMVKSLSDLARQLRIEVVAQGVASGKQL
                     EFLAKLGIPTAQGYLLGLPGPMDRVPLGVNLRQLVGTAVSEVERRAQGSLISAPGSEP
                     GAPTHGHDRRTVVRTEGDRRQVPGSGSPAAAGAGASAAHSSEAETPLAGRPALAHLVG
                     MPRRSESTAD"
     gene            complement(2390..2734)
                     /locus_tag="PLP95_06975"
     CDS             complement(2390..2734)
                     /locus_tag="PLP95_06975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB45580.1"
                     /translation="MSPTEKRGDDKPTRCAWCSRALPTRSPQAIGRRAIYCRRSCRQR
                     AYEARRRSAELGIGDDDLVITRNELDDANDRLFEIRLLAADARRDLDDRLAATRVLSR
                     LLDGIDAVIATN"
     gene            complement(2778..>3725)
                     /locus_tag="PLP95_06980"
     CDS             complement(2778..>3725)
                     /locus_tag="PLP95_06980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB45581.1"
                     /translation="HRLCNRLFVVDHRYSLRHDANGNDPCSSSGCPWGIATASSQCRR
                     GQISALWRYGDRMRYGVRSRKTLGAFVLCGAILGTALAGCGGEGSGADAKSAAGAAGA
                     VSTTAEVSTTVDVTSTTVEVTSDAVGSDGMASDEVASTTTLDPRSAVDESAPDSATAG
                     DVREFGGTPPAGPDALGELGALGALADGTYFGYVTAVDLRRSTIDFDAAQLLSGVAAI
                     EQALADGAVSADDPTPPGGLYVVNKSLAVRTLRTAPGLVVELLAGPSSAELVSASLVR
                     LENFVGLSTTVPDARGVTVQVVVANGQASRIVQHYLPTQ"
BASE COUNT          623 a         1140 c         1286 g          676 t
ORIGIN      
        1 gttgagatcg ccggacaacg ccgcgcccgc gctttgggcc agaatcgtca acgaggagcc
       61 cgaacggtcg attgcgtcga gcaacggacc gttcgggctc ctcgttgacg attctggccc
      121 aaagcgcggg cgcgtcgttg tcaggcgatc tcaacagccg tgccgaggcg caacgaggaa
      181 ccgcgctgcg gtacctctac agcgggatcg ccctggcctt ttctgcgacg gtggtgatct
      241 cgcttcgtca gaaccggaac cgtcaggccg atgaggacct gcgcacctcg gcgtcgaccg
      301 acgtgctgac cggtttggcg aaccgggcga tgcttgagtc gtgggtgcag gaccacgccg
      361 aggagtgcga cgagatcgtc ttgatgcaca tcgacctcga tcacttcaag ccggtgaacg
      421 acacctatgg ccacgccgtc ggtgaccggg tgttgcagat tgcagcgagc cggctctccg
      481 aggtcgccac caactacggc gggctcgctt cacgcatcgg tggcgacgag ttcgtggtgg
      541 tgttgccgcc ccaggacgtc gacgaccttg agcggatttc gggacgcctg gtcgattcgc
      601 tcatccatta tgaggtcggc ggtctcgatc tgaccgtcgg tgcgagtatc ggtgtggcgc
      661 gaggcaatgg ctcgtttcac gacctgttga tcgacgccga cctggcgctc tatcaggcga
      721 agcgaaccgg acgtgggcag gccagcacct tcaagtccga cgccgcggac ttcgtggact
      781 tcatccgtgc agaactcgat cacggccgtg tccgggccgt gtatcagccg caggtgtcgc
      841 tgcgtgacgg tcgctgcatc ggcgtcgagg tctttgcccg gatggtggac gagcagggca
      901 cgctgctgca ggccgatggt tggctgagca tcgtcgagtg gatcggccgc accgacgagc
      961 tgttcgaaca tgtgatcacg gcggtcgctg cggatgtgga tcgggtcaag gacctggcgt
     1021 gtcgcctgtg gttcaacgtg tcgccggcgg atctgatgcg tccgcggggt gcggagtggc
     1081 tgttggggca actcgcccgc cttgggctcg acccggaaca ggtgggtatc gagttgacca
     1141 acgtcgagga cttcaacgac ccgcggcgtc tgagcagcgt gctttcggaa atccgagccg
     1201 caggatacgg cattgcgctc gatgagttcg gtgcgaagga tgcgccactt gggcaccttc
     1261 gggacctgcc ggtgagccgg gtcaaattga attcgggtct ggtggcgcag gtgaacgagt
     1321 cgatgccccc gagtgcgtgg atggtgaagt cgttgtccga cctggcgcgg cagttgcgca
     1381 tcgaagttgt cgcccagggg gttgcgtcgg gcaagcagct ggagttcctg gcgaaactgg
     1441 ggattccgac cgcgcagggg tacctgttgg ggttgcccgg tccgatggat cgggtgccgc
     1501 ttggtgtgaa cctgcgacag cttgtgggaa ccgcggtgag cgaggtggaa cgacgcgccc
     1561 agggcagcct gatttccgcc ccgggttcgg agccgggagc cccgacccat ggccacgacc
     1621 gtcgcacggt ggtacgaacc gagggcgacc ggcgtcaggt gccgggctcc ggttcgccgg
     1681 ctgctgcggg tgcgggcgcg agtgcggcac actcaagcga ggccgagaca ccgctcgccg
     1741 gtcgtccggc gctcgcccac ctggtgggta tgccacgtcg ttcggagtcg actgccgact
     1801 gattgagcgg cgactgcgac ctgcagtcgc cgctgacgat cgctcggcgg atggtcggga
     1861 cgagcacctg acgtttggcg ggttcgacgg ccggctcaaa caccgtgctc aggttcgtca
     1921 gatgtctaac gcccttcttg gagcagcccc ggtgtttgca gtactcatga gtggggagag
     1981 ctccttatcg ttgtcggcgt cgaagcgcgt acacgttgtg acgatcgggc agccgttctc
     2041 tgcgaaggtg tgaccgtcgc agactcctac gcaatcgttg agactcggct ggaggccaca
     2101 tcggtggggg aggaccgtga cttggtgttc gtgtggctca ggacgcgagc tccgacagcg
     2161 ttcaagtttg gtgatgcgat tcaggagttg gcggggtgcc gcgaggcacc gttcggtcag
     2221 cgctggacga agcgcgtcat cgcggggcgg ttgagctccg cggaaccggc aggggagctc
     2281 attgggtcat cgtgaatcgc tccgccgtgg aatccgccgc ccaaacgata gcttgacata
     2341 atgggtattt acggcggtag tgccgcgaag cagaagagcg tgcagcccgt cagttggtgg
     2401 cgatcacggc gtcgattccg tcgaggaggc ggctcagtac gcgtgtcgct gcgagtcggt
     2461 catcgagatc ccgccgggcg tcggccgcca acaggcggat ttcgaacaat cggtcgttgg
     2521 cgtcgtcgag ttcatttctc gtgatgacca ggtcatcatc gccgattccg agttctgcgg
     2581 agcgacgccg ggcctcgtac gcacgctggc ggcagcttcg acggcaatag atcgctcgcc
     2641 ggcctatggc ttgaggtgaa cgtgtcggca acgctcgcga acaccaggcg caccgagtgg
     2701 gtttgtcgtc gccccgcttc tcggtggggc tcactctccc gctttgtttc gtcacagtga
     2761 cgaaacgtac cacgcgccta ctgggtcggg aggtaatgct ggacgattct cgaggcctga
     2821 ccgttggcga ccaccacctg gacggtcacc ccgcgagcgt cggggacggt cgtcgacagc
     2881 ccgacgaaat tctccagacg aacgagagag gctgagacca attcggcaga actcgggccg
     2941 gcgagcaact cgacgacaag cccgggcgca gtgcgaagcg tccgtaccgc caatgacttg
     3001 ttgaccacgt acagcccgcc gggcggtgtc gggtcgtcag cggaaacggc gccatcagcg
     3061 agcgcctgct cgatggccgc gacgccgctc agcagttggg ccgcatcgaa gtcgattgtc
     3121 gatcgcctca ggtcgacagc ggtgacatag ccaaaatagg taccgtcggc gagtgcgccc
     3181 agcgcgccga gttcgcccag cgcatcgggc ccagccggcg gggtgccgcc aaactccctg
     3241 acatcgccag cggtagcgct gtcgggggcg gactcgtcga cagcgctgcg cgggtcaagg
     3301 gtcgtcgtcg aagccacctc gtccgaagcc atcccgtccg atcccacagc gtccgaggtc
     3361 acctcgaccg tcgtcgaggt cacgtcgact gttgtcgaca cctcagccgt ggtcgacact
     3421 gcacccgcag cacccgcagc actcttcgcg tcggcgccgc tcccctctcc cccacatccg
     3481 gccaacgcag tgccgaggat ggcgccgcac agcacgaacg ccccgagggt tttcctgctg
     3541 cgcacgccgt atcgcatccg atcaccgtat cgccacaacg ccgatatctg gcctcgccga
     3601 cactgtgaag atgctgtggc gatgccccac ggacaccccg acgacgagca ggggtcgtta
     3661 ccgttggcgt catggcgcaa cgaatagcgg tgatcgacga cgaacaggcg attgcagagg
     3721 cggtg
//
LOCUS       DAOOMA010000221         3725 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_29937_length_3725_cov_2.966757, whole genome shotgun sequence.
ACCESSION   DAOOMA010000221 DAOOMA010000000
VERSION     DAOOMA010000221.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 3725)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3725)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3725
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29937_length_3725_cov_2.966757"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..640
                     /locus_tag="PLP95_06985"
     CDS             <1..640
                     /locus_tag="PLP95_06985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016079909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HPB45582.1"
                     /translation="GDYAFCSGGDQRIRGDQGYRGDDAVAQQGIGRLNVLDLQIQIRR
                     TPKPVVAMVAGYAVGGGHVLHVVCDLTIAADNAIFGQTGPKVGSFDGGYGSGLLAANI
                     GQKRAREIWMLCRQYDSTRAYEMGLVNQVVELEALEEETVQWCTEMLDMSPMALRMVK
                     ASCNAADDGLAGIQQLAGDATLLFYQTEEAQYGRDSYKNKEKPDFSRFPRRP"
     gene            637..1554
                     /locus_tag="PLP95_06990"
     CDS             637..1554
                     /locus_tag="PLP95_06990"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0046428 - 1,4-dihydroxy-2-naphthoate
                     octaprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HPB45583.1"
                     /translation="MSRTTFMSGVGQNRWVLGARPRTLPAAVVPVFVGTAAAWRVADR
                     LDWAHFVLALVVALALQIATNYANDYSDGKRGTDDPSKRVGPVRLVGNGLATPDEVKR
                     AMVVAFGVAAVAGSVLVLRVGWELVPVGVAAILAGYFYTGGPKPYGYLGLGEVFVFVF
                     FGLVATVGSTYVQIERIPALAWGCAVICGCYATAMLVVNNLRDIPGDTISGKRTLAVK
                     LGDVRTRFLYCALMIVPFLLLPFVAGLGGRPIAGLAFIAIIPAQKPIQAVLGGAKGPA
                     LIPVLGATGKVQLIFGVFLTAGLVVSTYI"
     gene            complement(1577..2311)
                     /locus_tag="PLP95_06995"
     CDS             complement(1577..2311)
                     /locus_tag="PLP95_06995"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HPB45584.1"
                     /translation="MTSTTTRAVLLHGFAQNRNCWGPLAGRLASERDVLALDLAGHGT
                     ASEVTADLWQSGQLVLDAIGDQPAAVIGYSMGARVALHAALTRPEAIDRLVLISGTAG
                     IDDPNEREQRRVRDEALAERIEDLGIDAFLDEWLALPMFAGLTDETRFLEQRRTNTAA
                     GLASALRHSGTGTQEPLWDRLGELTMPTLVIVGELDPTYEALGERLVDGIGDSSMFVV
                     VGNSGHSPHLEQPEAVIDLITTWAAG"
     gene            2340..>3725
                     /gene="menD"
                     /locus_tag="PLP95_07000"
     CDS             2340..>3725
                     /gene="menD"
                     /locus_tag="PLP95_07000"
                     /EC_number="2.2.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070204 -
                     2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-
                     carboxylic-acid synthase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-5-enolpyruvyl-6-hydroxy-3-
                     cyclohexene-1-carboxylic-acid synthase"
                     /protein_id="HPB45585.1"
                     /translation="MVSQNPDFVTTSAAATPAAFNADAFNAGATFAATLMDEWARCGV
                     RHVVVSPGSRSTPLAVAAATHPGLVTHVHHDERSASFLALGISLATQVPTVLVCTSGT
                     AAVEYHAAVVEAHHGNVALIVCTADRPPELQGVGAPQTIDQRGLYGSAVRWFAEPGPA
                     ELDAAAQWRPLAAEAHRRARGDRRCGDAPGPVHLNLAFREPLLGPIAPLPPLADDGAT
                     DVDAAPGDPAGCSEDEFAELAAQMAGRRGVIVCGDRTATDLVEAEAVAALAAALGWPV
                     FSDAQSRMRSDRRTGIAADVNAAGGAADDVALDAALVGVDPLLRVPEVAALGPPEVVV
                     RFGALLTSKVMYQWLADSAALQVGVDRWGAIPDPDHVIGRRVWAEPAAFAARLRDAVV
                     AVSGAAAPREWVATWRGLSGAAREVIDAHATGEVAVARAVLEELGAEDHLVVSSSMPV
                     RDLEWYGGGASA"
BASE COUNT          505 a         1203 c         1361 g          656 t
ORIGIN      
        1 gggtgactat gccttctgtt ccgggggcga ccagcgcatc cgcggcgacc agggctaccg
       61 cggcgacgac gcggtggcac aacaaggcat cgggcggctc aacgtcttgg acctgcagat
      121 ccagattcgc cgcaccccca aacccgtggt ggcgatggtc gccgggtacg ccgtgggtgg
      181 cggtcacgtg ttgcacgtcg tgtgcgacct gaccatcgcc gccgacaatg ccatcttcgg
      241 ccagaccggc ccgaaggtcg gctccttcga cggcgggtac gggtcgggcc tgctcgcggc
      301 gaacatcggc caaaagcgcg cccgggagat ctggatgctg tgtcgccagt acgactcgac
      361 gcgggcctat gagatgggcc tggtcaatca ggtcgtcgag ttggaggcgc tcgaggagga
      421 gacggtgcag tggtgcaccg agatgctcga catgtcgccg atggccctgc gcatggtgaa
      481 ggcctcgtgc aacgctgccg acgacggcct tgcgggcatc caacagctcg ccggcgacgc
      541 cacgctgctc ttctaccaaa ccgaggaggc gcagtacgga cgcgactcct ataagaacaa
      601 ggaaaagccc gacttcagcc gcttcccgcg gcgtccttga gccgcacgac gttcatgtcg
      661 ggagtcggac agaaccgatg ggtgctcggt gcacggccgc gcaccctgcc agcggccgtg
      721 gtgccggtgt ttgtcggcac cgctgccgcg tggcgcgtcg ccgatcgtct cgactgggcg
      781 catttcgtgc tcgcgctcgt cgtggcgttg gcgctgcaga tcgccacgaa ttacgccaac
      841 gactactccg acggaaagcg cggcaccgac gacccgtcca agcgggttgg tccggtgcgt
      901 ctcgtcggaa acgggctggc cacccccgac gaggtgaagc gggcgatggt cgtcgccttc
      961 ggtgtcgctg ccgtggccgg ttcggtgctg gtgttgcggg tcgggtggga actcgtgccc
     1021 gtcggcgtgg ccgccatttt ggccggctac ttctacaccg gcgggccgaa gccctatggc
     1081 tatctcggcc tcggcgaggt gttcgtattc gtcttcttcg gcctcgtggc gaccgtcggt
     1141 tcgacctacg tgcagatcga gagaattccc gccctcgcct gggggtgtgc ggtgatctgc
     1201 gggtgttacg cgaccgcgat gctggtggtc aacaaccttc gcgacatccc cggcgacacg
     1261 atctcgggca agcgcaccct tgcggtgaaa ctcggtgacg tgcgcacccg gtttctgtac
     1321 tgcgcattga tgatcgtgcc gttcctgttg ttgccctttg tcgccggcct cggagggcgg
     1381 ccgatcgccg ggctggcctt catcgccatc atccctgcgc agaagccgat tcaggcggtg
     1441 ctcggcgggg cgaagggccc ggcgctgatt ccggtgctcg gcgcgacggg caaggtgcag
     1501 ttgatcttcg gtgtgttcct gaccgccgga ctggtcgtgt ccacctacat ctgagccggc
     1561 tgggcggatg ggcggctcag cccgccgccc aggtggtgat gaggtcgatc accgcctcgg
     1621 gttgttcgag atgcgggctg tggccggagt tgccgacgac cacgaacatc gatgagtcgc
     1681 cgatcccgtc gacgaggcgt tctccgaggg cctcataggt cggatcgagt tcgccgacga
     1741 tcaccaacgt cggcatcgtc agttctccga gtcggtccca cagcggttcc tgggtgccgg
     1801 ttccgctgtg gcgcagcgcg gaggcgagcc cggctgcggt attggtgcga cgttgttcga
     1861 ggaagcgggt ctcgtcggtg aggccggcga acatgggcag cgcgagccac tcgtcgagga
     1921 aggcgtcgat accgaggtct tcgatccgct ccgcgagggc ctcgtcccgg acgcgtcgct
     1981 gttcgcgttc gttcgggtcg tcgatgccgg ccgtgcccga gatcaacacg agacggtcga
     2041 tcgcctcggg gcgggtgagt gccgcgtgca gggcgacgcg ggcccccatc gagtagccga
     2101 tgaccgccgc cggttggtcg ccgatggcgt cgaggacgag ttggccgctt tgccacaggt
     2161 cggcggtcac ctcgctcgcg gtgccgtggc cggcaaggtc gagggccaac acgtcacgtt
     2221 cggaggcgag tcggccggcg aggggtcccc agcagttgcg gttctgggcg aacccgtgca
     2281 acagcactgc cctcgttgtc gtggaagtca cgcaggcaac gtagtcgtag gctggggacg
     2341 tggtgtccca aaacccggat ttcgtcacga cctccgcagc ggcgaccccc gcagcgttca
     2401 atgccgacgc gttcaatgcc ggggcgacgt tcgccgcgac cctgatggac gagtgggcgc
     2461 gctgcggggt ccgccacgtc gtggtatctc caggatctcg cagcaccccg ctcgcggttg
     2521 ccgctgcgac tcacccgggc ctcgtcaccc acgtgcacca cgacgaacgc tcggcgtcgt
     2581 ttctggcgct cggcatctcg cttgcgaccc aggtcccgac agtgctggtg tgcaccagcg
     2641 gaaccgcggc ggtcgagtat cacgcggcgg tcgtcgaggc tcatcatggc aacgtcgcgc
     2701 tcatcgtgtg tacagctgat cggccgccgg aacttcaggg ggtcggcgcg ccacagacga
     2761 tcgatcaacg ggggctgtac ggttcggcgg tgcgctggtt tgcagagccc ggcccggccg
     2821 aactcgatgc cgcagcgcag tggagaccgc ttgcagccga ggcccaccgc cgggcgcgtg
     2881 gtgaccggcg ctgtggcgac gctccagggc cggtgcatct caatctcgcg tttcgcgagc
     2941 cgctgctggg tccgatcgcc ccgttgccgc cgctcgccga tgacggggcc accgacgtcg
     3001 atgccgcgcc cggcgacccc gccggctgtt ctgaggacga gttcgcggag ctggcggcgc
     3061 agatggcggg gcgccgtggg gtgatcgtgt gcggcgaccg tacggccacc gatctcgtgg
     3121 aggccgaggc ggtcgcagcg ttggcggcgg cgctgggctg gccggtgttc agcgacgcgc
     3181 agtcccgtat gcgttcggac cggcgtacgg gcatcgctgc ggatgtcaac gctgcgggcg
     3241 gcgccgctga cgacgtggcc ctcgacgctg cactcgtggg tgtcgacccg ctgttgcgcg
     3301 tgccggaggt ggcggcgttg ggcccgccgg aggtcgtggt ccgtttcggc gccctgttga
     3361 cctccaaggt catgtaccag tggctcgccg acagcgctgc gctccaggtc ggcgtcgatc
     3421 gttggggcgc gatacccgat ccggatcacg tcatcggccg gcgggtgtgg gccgagcccg
     3481 cggcattcgc cgcgcggctg cgtgacgccg tggtggcggt ttcgggtgcc gccgcgccac
     3541 gggagtgggt ggcgacctgg cgagggctga gcggcgccgc ccgggaggtg atcgacgccc
     3601 acgccaccgg ggaggtggcg gttgcgcgtg cggtgctcga ggaactcggc gccgaggatc
     3661 atctcgtggt gtcgtcatcg atgccggtgc gcgacctcga atggtacggc ggtggcgctt
     3721 cggcc
//
LOCUS       DAOOMA010000222         3722 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_29980_length_3722_cov_4.269157, whole genome shotgun sequence.
ACCESSION   DAOOMA010000222 DAOOMA010000000
VERSION     DAOOMA010000222.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 3722)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3722)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3722
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29980_length_3722_cov_4.269157"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            290..1504
                     /locus_tag="PLP95_07005"
     CDS             290..1504
                     /locus_tag="PLP95_07005"
                     /inference="COORDINATES: protein motif:HMM:NF016777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HPB45586.1"
                     /translation="MSDASTTATGTLRPFDADNHYYEALDAFTRHLPAGWEQRCIQWA
                     DIGGRKYHVVGGKVSYAVVNATFDPVAKPGVLAEYFRGNPNGDDPIELLKAREPIHPC
                     YRDREARIAQLDEHGLDAVWMFPTLGMIYEEQLRHDPEAVAVAFKAFNRWVLDDWGFN
                     FKDRIFAAPYISLARIDAAVDELRFALDHGATTVVMRPAATTTAGGMRPPTAPEFDPF
                     WALANEAGITVVVHAGDSGYSSNGYADDGFTSNFKGGVPQPIKFTQLERPIEDFLASL
                     VCDQHFAKFPNLRIASVENGSGFLRGLFSKLDATANKMAGWFADHPVDAFKEHIWINP
                     FWEDDISEVVDLVGADRVLFGSDWPHIEGMPEPLDYLRELEGLTSEDVSKIVRGNVVE
                     LNSLRPVPPTPR"
     gene            complement(1511..2638)
                     /locus_tag="PLP95_07010"
     CDS             complement(1511..2638)
                     /locus_tag="PLP95_07010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012223680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB45587.1"
                     /translation="MAVALDDFPYHQAPVSMEFVASSDRNFYDRYYFNAHDRTGDIFV
                     ISGLGIYPNLGVVDAFVTVRIGDRQHTVRFSDAHDGNREPRVGPYRIEVVEPLRTLRI
                     VSDSDAHGVGVDLTWNGMFPVVDEAPHQLRHDGRLILDAQRFAQVGTWSGEIRVEGRT
                     ITVDPEKWIGTRDRSWGIRPVGEPEPAGRSAAEADPHYGFWWTYLPFGFDDFTVVVIA
                     QEDGAGIRSLSDAVRVWGPGSDRGRELLGWPEFRYTYRPGSRDVQQVEVSLRDVDGSP
                     MTMVCESLGYVALNSGPGYGGDPDWGHGQWRGRNWAEGVVADMTDPEVLGRLPFGVVD
                     HVGRAVLTEASGATHEGWGLLEHGTIGAHAPTGFTDFFTVA"
     gene            2733..>3722
                     /locus_tag="PLP95_07015"
     CDS             2733..>3722
                     /locus_tag="PLP95_07015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="circularly permuted type 2 ATP-grasp protein"
                     /protein_id="HPB45588.1"
                     /translation="MTFDDYEMTSFDELIAPDGAPRAEAQALVDHLERLGIDEIAMRQ
                     HAADVGIEAAGITFTVYSEGAGIDRAWPFDIVPRVIARRTWDRIDAGLVQRLQALNRF
                     IDDVYNDRRIFAEGIVPWSLVENSPGYRRQCEGVHPAHGVWAHICGSDLVRGDDGTFY
                     VLEDNLRVPSGVSYMLENRETTKRVFPELFANQSILPVDAYPDRLRRVLGSLSPEGET
                     ATIAVLTPGVYNSAYFEHSFLARQMGVALVEGNDQQVDDDLLVTMRTIGGPVPVDVIY
                     RRVDDEFLDPLAFRPDSALGCPRLMEAWRAGTVAIANAPGTGVADDKAVYAYVP"
BASE COUNT          663 a         1277 c         1163 g          619 t
ORIGIN      
        1 cgacccgcaa cagatcgcgt cggttcaact tcgtcactgc gcccctcccg gtcacctcag
       61 taccgtccct cggttccatt ccccgtacgg acctggccac cggattcggg ggacagactc
      121 gcaccacgac gttgaccccg aagtcgtggc ggcgtcaagc gcgtcccggt cggagtcgga
      181 cgacgcccct gcaatgcagc gacgtcgtgg cgacgccgca ccctcacgca cccagcgcgc
      241 ggtgaatgcg cggttggccg cacggtcgac tgcgcaccaa gctggagcga tgagcgatgc
      301 ctcgaccact gccacaggga cgcttcgccc cttcgacgcc gacaaccact actacgaagc
      361 cctcgacgcg ttcacccgcc acctccccgc gggatgggag cagcgttgta tccagtgggc
      421 cgacatcggt gggcgcaaat accacgtcgt cggcggcaag gtgagctatg cagtggtcaa
      481 cgcgacgttc gaccccgtgg cgaaaccggg cgtgctagcg gagtacttcc gtggcaatcc
      541 gaacggtgac gacccgatcg agttgttgaa ggcacgcgag ccgatccacc cgtgctatcg
      601 agaccgcgag gcgcgcatcg cccaactcga cgagcacgga ctcgatgcgg tgtggatgtt
      661 cccaacgctc gggatgatct acgaggagca gttacgccac gaccccgagg ccgtggcggt
      721 ggcgttcaag gcgttcaacc ggtgggtgct cgacgactgg ggcttcaact tcaaggaccg
      781 gatcttcgcg gcgccgtaca tcagcctcgc ccgcatcgac gcggcggtcg acgaactccg
      841 gttcgcgctc gaccacgggg cgacgacggt ggtgatgcgt cccgcggcga cgaccaccgc
      901 tggcgggatg cggccaccca ccgcccccga gttcgatccc ttttgggcat tggcgaacga
      961 ggccgggatc accgtcgtcg tgcatgccgg cgattcggga tacagctcca acggctacgc
     1021 cgacgacggg ttcacctcca acttcaaggg tggggttccc caaccgatca agttcaccca
     1081 actcgaacga ccgatcgagg acttcttggc gtcgctcgtg tgcgatcagc acttcgccaa
     1141 gttccccaac ctgcgcatcg cgtcggtcga gaacgggtcg gggttcctgc gcgggctgtt
     1201 ctccaagctc gacgccacgg cgaacaagat ggcgggctgg ttcgccgatc accccgtgga
     1261 cgcgttcaaa gagcacatct ggatcaaccc gttctgggag gacgacatct ccgaggtcgt
     1321 cgatctggtc ggcgcggatc gggtgctgtt cgggtcggac tggcctcaca tcgagggcat
     1381 gcccgaaccg ctcgactacc tgcgcgaact cgaagggctc acgagcgagg acgtttcgaa
     1441 gatcgtgcgc ggcaacgtcg tcgaactcaa ctccctgcgc ccggttccgc ctacgccacg
     1501 gtgaatccac ctacgccacg gtgaagaagt ccgtaaaccc ggtcggtgcg tgagcgccga
     1561 tcgtgccgtg ttcgagcagg ccccacccct catgggtcgc tcccgatgcc tcggtgagca
     1621 ccgctcgccc aacgtggtcc accacgccga agggaagccg accgagcacc tcggggtcgg
     1681 tcatgtcggc caccacgccc tcggcccagt tgcggccgcg ccattggccg tgtccccagt
     1741 cggggtcgcc gccgtatccg gggccgctgt tgagcgccac gtacccgagc gactcgcaca
     1801 ccatcgtcat cggcgagcca tcgacatcgc gtagcgacac ctcgacctgt tgaacatcgc
     1861 ggctgccggg gcgataggtg tagcggaact cgggccaccc gagcagctct cgacctcgat
     1921 ccgagcccgg cccccacacc cgtaccgcat cgctgagcga acggatcccc gctccgtcct
     1981 cctgggcgat caccacgacg gtgaagtcgt cgaatccaaa gggaaggtag gtccaccaga
     2041 acccgtaatg agggtctgcc tcggcggcgg aacgaccggc cggctcgggc tcaccgaccg
     2101 gacggatccc ccacgaacgg tctcgggtcc cgatccattt ctcgggatcg acggtgatgg
     2161 ttcggccctc gacgcggatc tctcccgacc acgtgccgac ttgggcgaac cgttgggcgt
     2221 cgaggatcaa ccggccgtcg tggcgaagct gatgcggggc ctcgtcgacc acggggaaca
     2281 tgccgttcca ggtcaggtcc actccaacgc cgtgagcgtc gctgtcggac acgatgcgaa
     2341 gcgtccggag cggttcgacc acctcgatcc ggtacggacc aacgcgtggt tcgcggttgc
     2401 cgtcatgcgc gtcggagaac cgaaccgtgt gttgacgatc gccgatccgc accgtcacga
     2461 acgcgtcgac gactccgagg ttggggtaga taccgagccc gctgatcacg aagatgtcgc
     2521 cggtgcggtc gtgggcgttg aagtagtaac ggtcgtagaa gttgcgatcg ctcgacgcga
     2581 cgaactccat cgaaaccgga gcctggtgat acggaaaatc gtcgagcgct acagccatgg
     2641 ctcacggttt accaaaccca ccgcgcctca tgttcacggg gccgtaacca ccctcgaatc
     2701 gccgatacac atcgccgact tagaacgcag tcgtgacctt cgacgactac gagatgacga
     2761 gcttcgatga gctgatcgct cccgacggcg caccacgcgc cgaagcccaa gcgctcgtcg
     2821 atcacctcga acgtctcggg atcgatgaga tcgcgatgcg tcaacacgct gcggacgtcg
     2881 gaattgaagc ggcaggaatc accttcaccg tctactccga gggtgccggt atcgatcgtg
     2941 catggccctt cgacatcgtc ccccgcgtca tcgcccgacg cacgtgggat cgaatcgacg
     3001 ccgggttggt ccagcggctg caggcgctca accgcttcat cgacgatgtc tacaacgacc
     3061 gccgaatctt cgccgagggc atcgtgccgt ggagcctcgt cgagaactcc cccggatacc
     3121 gccgccaatg cgagggggtt catccggccc acggggtgtg ggcccacatc tgcggcagcg
     3181 acctcgtgcg tggcgatgac gggaccttct acgtcctcga ggacaacctg cgtgtcccct
     3241 cgggggtctc ctacatgctc gagaaccgcg agaccaccaa acgggtcttt cctgagctct
     3301 tcgccaatca gtcaatcctg ccggtggacg cctatcccga tcgtctccga cgggtgctgg
     3361 gatcgctcag ccccgaggga gagacggcga cgatcgccgt gctcacgccg ggggtgtaca
     3421 actccgcgta cttcgagcat tcgtttctcg cccgccagat gggcgtcgcc ctcgtcgaag
     3481 gtaacgacca acaggtcgac gacgacctgt tggtcacgat gcgcacgatc ggcgggccgg
     3541 tgccggtgga cgtcatctac cgacgcgtcg acgacgagtt tctcgatccg ctcgcctttc
     3601 ggcctgattc cgcgctcggg tgcccccggt tgatggaggc ctggcgcgcc ggcaccgtcg
     3661 cgatcgcgaa cgcccccgga accggggtcg cggacgacaa ggcggtctac gcgtacgtgc
     3721 cc
//
LOCUS       DAOOMA010000223         3713 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_30073_length_3713_cov_4.450793, whole genome shotgun sequence.
ACCESSION   DAOOMA010000223 DAOOMA010000000
VERSION     DAOOMA010000223.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 3713)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3713)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3713
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30073_length_3713_cov_4.450793"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            186..896
                     /locus_tag="PLP95_07020"
     CDS             186..896
                     /locus_tag="PLP95_07020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB45589.1"
                     /translation="MMTSETLQGTGLQGTGLDSVIGMYAVVHRLRSTVVTPLVAAGLG
                     DPLVRAPKRVLVYGPPQCGVRFIADCLAAELEEMPGVSVAVLPCLDDDPFTTSVNEAV
                     AMAAGTHTVVVATSHQPWTLDGELLDGFDRTCFVHPPDWEARRFRLWESHLGRLVDTD
                     ALDNFVTVTEGWCGTDLAAGFESVDTSDLDASVATAHQTGAPVTAQWLDDAVQLIRER
                     SGAGFDDLKSYLQRVRRW"
     gene            complement(925..1632)
                     /locus_tag="PLP95_07025"
     CDS             complement(925..1632)
                     /locus_tag="PLP95_07025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB45590.1"
                     /translation="MKRMLVLIMFPLSLVVVAVLSGAFSQRFDAAVTLKSDDIRASRN
                     IVIARLSELGASIRAEDTRGADASEVIFQVPAAQLNAVLDTLNAVGDVTSTEVDLSGS
                     FDDAPSVASGLAELDGCIDRVSNQLGENAVTSARSSLDECRARLDRTIAKADAEPHSA
                     EPVRLAVRFTANGSSWWTVAVATAIAFLLLVVAGAAFVFARRQRADNIVVLPRDDEPH
                     RITDRSHERSPGHGHWS"
     gene            complement(1693..2466)
                     /locus_tag="PLP95_07030"
     CDS             complement(1693..2466)
                     /locus_tag="PLP95_07030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012227961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HPB45591.1"
                     /translation="MESIDFTGKVAVVTGGTKGIGRTIVERFLALGAQVMTCGRSEPE
                     SLPTSGDQSAAFFACDVRDPEQVQAFVDATVERFGRLDIAINNAGGSPHVNAADASPR
                     FSESIIKLNLLAPLYLATAANAVMQKQQDGGTIINITSVSGIRPSPGTAAYGAAKAGL
                     LSLTTSLAIEWAPKVRVNAVTCGLIETDQAELHYGDAEGIARVAATVPLRRMGTPDDV
                     ANACVFFASPLAAYANGSQVLLHGGGELPAFLNAADLHV"
     gene            2590..3348
                     /locus_tag="PLP95_07035"
     CDS             2590..3348
                     /locus_tag="PLP95_07035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020379403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase family protein"
                     /protein_id="HPB45592.1"
                     /translation="MPITCTITDGIAEIVMDNPPVNALTVAGWFELGEVITAAGRDPE
                     VGAVILRAEGKGWNAGVDIKEMQNTEGFDALIGANRGCYAAFAAVYECEVPVIAAVHD
                     FCLGGGIGLVGNADIIVAAEGTRFGLPEVNQGALGAATHLARLVPQHKMREMVYTALP
                     VDAQYLADYGSVTRVVPLEELRDAAFEIARVICTKSRLVIRRAKESLNAIDPVDVKRS
                     YRFEQGFTFELNLSGVSDEARDAFVEKRDASYSK"
     gene            complement(3429..>3713)
                     /locus_tag="PLP95_07040"
     CDS             complement(3429..>3713)
                     /locus_tag="PLP95_07040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB45593.1"
                     /translation="SEQSLSPTDEAGARAAGKFFAQTYRRLGQLTEGLTPETREAIAK
                     LADEIEAEATADDLDVAAYVDHGPTMLANEEYVAAQTELNTRLGAQCAAE"
BASE COUNT          594 a         1191 c         1269 g          659 t
ORIGIN      
        1 tttctagggg ggtagcactg ctgggaggtg ctgttggtct ggttgtcgct ctgggccgag
       61 cgaacgatgg acagagggta ccgcggcccc ctccggtagg cgccaagtgc aaccgcgctg
      121 agccggttga ttcaaatgca ttccggcgtg cctcatcgcg accgcccccc tctaccgtga
      181 cgcgcatgat gacctctgag acccttcagg gaaccggcct ccagggcacc ggtctcgatt
      241 ccgtgatcgg catgtacgcg gtcgtgcatc gacttcgttc gacggtggtg acgccgctgg
      301 ttgccgccgg cctcggagac ccgctggtgc gcgccccgaa gcgagtcctg gtctacgggc
      361 cgccgcagtg cggtgtgcgg ttcatcgccg actgtctggc cgcagagctc gaggagatgc
      421 cgggggtcag cgtcgccgtg ctgccgtgcc tggacgacga cccgttcacg acctcggtga
      481 acgaggcggt cgccatggcc gcaggcaccc acaccgtggt cgtggcgacg agccatcagc
      541 catggacgct cgacggcgag cttctcgatg ggttcgaccg cacgtgtttc gtgcatccac
      601 cggattggga ggcgcgccga ttccggctgt gggaatcgca cctcggacgc ctcgtcgaca
      661 ccgatgcgct cgacaatttc gtgacggtca ccgaaggctg gtgcggcacc gaccttgcgg
      721 ccgggttcga atcggtggat acgtcggatc tcgacgcctc ggtcgcaacc gcccaccaga
      781 cgggcgctcc ggtgaccgcc caatggctcg atgatgcggt gcagctcatc cgtgagcggt
      841 ccggcgcagg attcgacgac ctgaagtcgt atctccaacg cgtacggcgc tggtagcggc
      901 tccctccggg ccaggccgtt gcggtcagct ccagtgcccg tggccgggcg aacgttcgtg
      961 ggaacggtcg gtgatgcgat ggggctcgtc gtctcgcggt aataccacga tgttgtcagc
     1021 ccgttgacgt cgagcgaaca cgaacgccgc accagcgacg acgagcagta ggaacgcaat
     1081 ggccgtcgca acggccaccg tccaccaact cgagccgttg gcggtgaagc gcacggcgag
     1141 gcgaaccggc tccgcgctgt ggggctcggc gtcggccttg gcgatcgttc gatcgaggcg
     1201 ggcacggcac tcgtcgaggg aggaacgcgc cgaggtcacg gcattttccc cgagctggtt
     1261 cgagacccgg tcgatgcacc cgtcgagttc cgccagcccg gacgcgaccg acggggcgtc
     1321 gtcgaaggaa ccgctgagat cgacctcggt gctggtcacg tctccgaccg cgttgagcgt
     1381 gtcgagcacg gcgttcagct gagcggccgg gacttggaag atgacttcgg aggcatctgc
     1441 ccctcgagtg tcttcggccc ggatcgatgc tcccaactcg gacagtcggg cgatcacgat
     1501 gttgcgcgat gcccgaatgt cgtcgctctt gagcgtgacc gccgcatcga agcgctggga
     1561 gaacgcgccg ctcaacaccg cgaccacgac gagtgagagc gggaacatga tcagcacgag
     1621 catccgcttc atcgatttga cactacggcg ccgagaaccg gcttcggcgg acctccgtgg
     1681 ttcggcgacg gttcagacgt ggaggtcggc ggcgttgagg aaggcgggga gttcgcctcc
     1741 gccgtgcaac agcacctgag acccgttcgc ataggccgcc agcggggatg cgaagaacac
     1801 acacgcgttg gcgacgtcgt ccggggtgcc catgcgtcgc agcgggacgg ttgcggccac
     1861 cctcgcgatg ccctcggcgt cgccgtagtg aagctcagcc tggtcggttt cgatcagccc
     1921 gcacgtcacg gcattgaccc ggaccttggg cgcccattcg atggccaacg aggtcgtcag
     1981 actcaacagg ccggccttcg ccgctccgta cgccgccgtg ccgggagatg gccggatgcc
     2041 ggacaccgag gtgatgttga tgatcgttcc gccgtcctgc tgtttctgca tcaccgcgtt
     2101 cgcggcggtt gcgagataca gcggggcgag caggttgagc ttgatgatcg actcgctgaa
     2161 tcggggtgag gcgtcggcgg cattgacgtg tggacttccg ccggcgttgt tgatcgcgat
     2221 gtcgaggcgt ccgaagcgtt ccacggtcgc atcaacgaat gcctgcacct gttcggggtc
     2281 gcgcacgtcg caggcgaaga acgcggcgct ttgatctccc gatgtcggta gcgattcggg
     2341 ttcgcttcgg ccacaggtca tcacctgcgc gccgagcgcc aggaaccgct cgacgatcgt
     2401 gcggccgatg cccttcgtgc cgccggtgac gacggcgacc ttacccgtga agtcgatgct
     2461 ctccatgggg gcgagggtat cgaggcttcc gcgggcgggt gaaacctgtt ctagatctcc
     2521 gggtctcggg tcgggaacaa tggggtgacc attccaccac ccggccgtcg cggccgatga
     2581 ggagatttcg tgcctattac gtgcaccatc accgacggca tcgccgagat cgtcatggac
     2641 aacccgccag tcaacgcgct caccgtcgcc ggctggttcg aactcggcga ggtgatcacc
     2701 gccgccgggc gcgaccccga agtcggcgcc gtcatcctgc gcgccgaagg caagggatgg
     2761 aacgccgggg tcgacatcaa agagatgcag aacaccgagg ggttcgacgc gttgatcggt
     2821 gcgaaccgcg gctgttacgc agcgtttgcc gccgtctatg agtgcgaggt gccggtgatc
     2881 gctgcggtgc acgatttctg tctgggcggc ggcatcggcc tcgtgggcaa tgccgacatc
     2941 atcgtcgcag ccgaaggtac ccggttcggg cttcccgagg tgaaccaggg agccctcggc
     3001 gccgccaccc accttgcccg tctcgtgccg cagcacaaga tgcgcgagat ggtctatacg
     3061 gcgctgccgg tcgatgccca gtacctggcc gactacggct cggtgacccg cgtcgtgccg
     3121 ctcgaggaac tgcgcgacgc ggcctttgag atcgcccggg tgatctgcac caagtcgcgg
     3181 ctcgtgatcc gccgtgccaa ggaatcgctc aatgcgatcg accccgtcga cgtgaagcgg
     3241 tcgtaccgct tcgaacaggg tttcaccttc gaactcaacc tgtcgggcgt gtccgacgag
     3301 gcccgtgacg cgttcgtgga aaagcgcgac gcgtcctatt cgaagtaacg cggcagcctc
     3361 ggcgggcgaa taacgcgttg agggcggccg cagtggccgc cctcaacgcg tttcagctca
     3421 gctgtaacct actcggccgc gcattgggcg ccgagtcgtg tgttgagttc ggtctgcgcg
     3481 gcgacgtatt cctcgttggc caacatcgtc ggcccgtgat cgacataagc ggcgacatcg
     3541 aggtcgtcgg cggtcgcctc ggcttcgatc tcatcggcga gcttcgcgat ggcttcgcgg
     3601 gtctcgggcg tcagcccctc ggtcagctgc ccgaggcgcc ggtaggtctg ggcgaagaac
     3661 ttgcccgcag cgcgggctcc ggcctcatcg gtcggggaca acgactgctc cga
//
LOCUS       DAOOMA010000224         3713 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_30081_length_3713_cov_3.197649, whole genome shotgun sequence.
ACCESSION   DAOOMA010000224 DAOOMA010000000
VERSION     DAOOMA010000224.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 3713)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3713)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3713
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30081_length_3713_cov_3.197649"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(1..1176)
                     /gene="lhgO"
                     /locus_tag="PLP95_07045"
                     /pseudo
     CDS             complement(1..1176)
                     /gene="lhgO"
                     /locus_tag="PLP95_07045"
                     /inference="COORDINATES: protein motif:HMM:NF013434.2"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-2-hydroxyglutarate oxidase"
     gene            complement(1285..3330)
                     /locus_tag="PLP95_07050"
     CDS             complement(1285..3330)
                     /locus_tag="PLP95_07050"
                     /inference="COORDINATES: protein motif:HMM:NF012430.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPB45594.1"
                     /translation="MADTSPQAYDEHSIQVLEGLDAVRKRPGMYIGGTGPEGMHHLVW
                     EIVDNAVDEAAAGHCNLIEVTLHKDRSVEVSDNGRGIPTGLKDGKRTAVEYVFTELHA
                     GGKFGAGAYAASGGLHGVGASVVNALSTKVTVEVDRDGLTQRLGFKDRKPGHFGPNGG
                     FKATSKLEQVKKIPAKRTGTRVRFWPDFEVFDPGLTIEYELVREHVAQVCFLVPGLKV
                     RLIDKRGSEPREPEEFIAKGGLADFVELLSIGEAVSEILTIFGTSTFTEKVPVEGKMT
                     EVTRPCQVDIAFRWVKGYETKLVSFVNTIPTAEGGTHVAGFERAMNKVANDVLLATTK
                     KLAKLAKQGKDRAEKTDVQEGLVAAVKVTFPEPQFKGQTKGELGTPPVQSIVYEAVKD
                     GFTNWIESGGRKTHVNAVRDKIAQAVLNRVATKQNLDAKRKAANLSSTGMPDKLADCR
                     THGIGSELLIVEGDSAAGPAKAGRNSEYMAVLPLRGKVVNAGKSTLKQVIENAEAQAL
                     ITAIGAGSGREFNIDDARYGRIIILCDADVDGSHIRCLLLTLIYHYMRPMLEDGRVFA
                     AQPPLYTVKVGDQKYHAYSDDEKDALTAELCKGNRRAENLQWVRFKGLGEMDVEELAE
                     SCLDVETRILRRITMDDAAEALRVAEVFDTLMGSDVARRRQFLLENSSLIDLEALDV"
     gene            complement(3387..>3713)
                     /locus_tag="PLP95_07055"
     CDS             complement(3387..>3713)
                     /locus_tag="PLP95_07055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB45595.1"
                     /translation="QPMACAMGTSSRPTLRFASAHARLTCASARISSEWIGWPRSWKF
                     RDERRVDFAYVGPDAAGLSVIVGTEDNPSKPDPNPESLTIAPSARDLISRPVSRRILD
                     VGFGRW"
BASE COUNT          586 a         1114 c         1285 g          728 t
ORIGIN      
        1 gtggccgatg gcgaggcttg ccgtcgccgc cgggctcgga gcgttcacaa cgtggaccga
       61 ccgagggcca tcggcgaact cgaaatcgtc gacaagggtt ccatcaggtc ggatggcctg
      121 ggcgcggatt ccggccttgc tcttgatcag gtcggccgcg gtcacctcgg gaacgagacc
      181 ctgcagcgcg tggacgaacg cggacttgct caacgagcgg tacatctcgc ccatgccggt
      241 cttccagtac ttctttgcca gcacccaact gcctttatcg aacgccatct ccttgatgtc
      301 gtcgaaatct gcgacgcccc acgtgtagcc ctcgcgtgcc agcgcgatca cggcattggg
      361 gcccgcatga atctcgccgt cgatcatgcg ggtgaaatgt actccgagga aggggaagcg
      421 tgggtcggga accggataga tgaggtgtcg gacgagactg cgcgactgcg gggtgagttc
      481 gtagtactcg ccgcggaacg gcatgatccg ggctcgggta tcgcgtccgg cgagctgggc
      541 gaccttgtcg gattgcaggc ccgaacagtt gatcatcgtc cgagcggtga cgacgcggct
      601 gtcggtgcgc accgtcacct agtcggcgga ctcggtgatg gcggtcgcag gggtgtcgaa
      661 gagcacctcg cctccggcgg cccggatctc ctcgaccagc gcgttacaca tcgccttgta
      721 gtcggtgatg ccagcttcgg gcaccagcag cgccgagagc ccttcgcagt gaggttcgcg
      781 ttcgcgaagc tgggcgcggg tcatcgtcga gatgttcagg ccgtgttcca ggccccgctg
      841 gtggagcgcg tcgagcttgt cgagttggtc ggtcgcggtg gccacgatca ctttgccgca
      901 cacgtcgacc ggaatggagt gctcgtcaag gaatgcgaac agcaactcgc ggccgctctt
      961 gaccatggcg gccttgttcg aacccggcgg gtagtagatg cccgagtgga tcacgcccga
     1021 gtttcggccg gtctgatgcg cggcagcctt ggattccttt tcgatgaccg tgacccgcgt
     1081 gtccgggcga agctgttgaa cggcgcgggc ggaggccagg ccgacgatgc cggcgccgat
     1141 gacgacgagg tcgaggtccg tggacgtggc ggtcatggtg gggaatactc cggatgggaa
     1201 ggcggggaac tgagcggctt tcacgctagc ggtggttggg aaaaccgcga cgttgtgtga
     1261 ccgaagcgat gcggggaccc cgcgttacac gtcgagcgct tcgaggtcga tgagtgagct
     1321 gttctcgagc aggaactggc ggcgacgggc gacgtcggaa cccatgaggg tgtcgaacac
     1381 ttcggcaacg cgcagcgcct cggcggcatc gtccatcgtg atgcgccgta ggatgcgcgt
     1441 ttcgacgtcg aggcaggact cggcgagttc ctccacgtcc atctcgccga gccccttgaa
     1501 gcgcacccat tgcaggttct cggcgcgacg gttgcccttg cagagttccg cggtgagcgc
     1561 gtccttctcg tcgtcggagt aggcgtggta tttctggtcg ccgaccttga cggtgtacag
     1621 cggcggctga gcggcgaaca cccggccgtc ctcgagcatc ggacgcatgt agtgatagat
     1681 cagcgtcaac aacagacagc gaatgtggct gccgtcgacg tcggcatcgc acaggatgat
     1741 gatgcggccg tagcgggcat cgtcgatatt gaattcgcga cccgaacccg cgccgatcgc
     1801 ggtgatgagc gcctgagcct cggcattttc gatgacctgc ttgagcgtcg actttccggc
     1861 gttgaccact ttgccgcgca atggcagcac ggccatgtac tcggagttgc ggccagcctt
     1921 ggccggcccg gcggccgagt cgccctcgac gatgaggagt tctgagccga tgccgtgggt
     1981 gcggcaatcg gcgagcttgt cgggcatgcc ggtcgaggag aggttggcgg ccttgcgctt
     2041 ggcgtcgagg ttctgtttcg tcgccacgcg gttgagtacg gcctgcgcga tcttgtcgcg
     2101 cacggcgttg acgtgggtct ttcgcccgcc gctctcgatc cagttggtga acccgtcttt
     2161 gacggcctca tagacgatcg actgcacggg cggggtgccg agttcgcctt tggtctgacc
     2221 tttgaactgg ggctcgggaa aggtgacctt caccgcggcg accagacctt cttggacgtc
     2281 ggtcttctcg gcgcggtcct tgccttgttt cgccagcttc gcgagcttct tggtggtcgc
     2341 cagcaacacg tcgttcgcga ccttgttcat cgcccgctcg aacccggcca cgtgggtgcc
     2401 gccttccgcc gtggggatcg tgttgacgaa gctgacgagc ttggtctcgt agcccttcac
     2461 ccagcgaaag gcgatgtcga cctggcacgg ccgcgtcacc tcggtcatct tgccctcgac
     2521 cggcaccttc tcggtgaacg tgctggtgcc gaagatggtg aggatctcgg agacggcttc
     2581 gccgatgctc agcaactcca cgaagtcggc caagccgccc ttggcgatga actcctccgg
     2641 ttcgcgcggc tcgctgccgc gcttgtcgat gagacgtacc ttgagtccgg gcacgaggaa
     2701 acacacttgg gcgacgtgtt cgcgcacgag ttcgtattcg atcgtgaggc ccgggtcgaa
     2761 gacctcgaaa tcgggccaga aacgaacccg cgtgccggtg cgtttggcgg ggatcttctt
     2821 gacctgctcg agcttcgagg tggccttgaa cccgccattc ggtccgaaat gtcctggctt
     2881 gcggtccttg aatccgagcc gttgggtgag tccgtcacgg tcgacttcga cggtgacctt
     2941 cgtggacagc gcgttgacga ccgaggcgcc gacgccgtgc agacccccgg acgcggcgta
     3001 cgcgccggcc ccgaactttc caccggcgtg aagctcggtg aagacgtatt cgaccgcggt
     3061 gcgcttgccg tccttgagcc cggtggggat acctcgccca ttgtcggaga cctcgacgga
     3121 gcggtccttg tgcagggtga cttctatgag gttgcaatgg ccggcagcgg cctcatcgac
     3181 ggcattgtcg acgatttccc agacgaggtg atgcatgcct tcgggtccgg tgccgccgat
     3241 gtacatgccg gggcgcttac gcaccgcgtc gagcccttcg aggacttgga tggagtgttc
     3301 gtcgtatgcc tggggggagg tatcggccat gagaagtcag ttctgagggt tggtcgagtg
     3361 aaggtgggcg atgtgtcgag gacgggtcac catcgcccga acccgacgtc gaggatgcga
     3421 cgcgaaaccg gtcgactgat gaggtcgcgg gcgctcggag cgatggtcaa tgattcgggg
     3481 ttggggtcgg gttttgaggg gttgtcctcg gtgccgacga tcacgctgag gccggcggcg
     3541 tcgggaccga cgtaggcgaa gtcgaccctg cgctcgtcgc ggaacttcca gctcctcggc
     3601 cagccgatcc actccgagct gatccgcgcg ctcgcgcagg tcaagcgcgc gtgtgccgag
     3661 gcgaaccgaa gggtcggcct gctcgaggtg cccatcgcgc aagccatcgg ctg
//
LOCUS       DAOOMA010000225         3711 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_30105_length_3711_cov_3.487144, whole genome shotgun sequence.
ACCESSION   DAOOMA010000225 DAOOMA010000000
VERSION     DAOOMA010000225.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 3711)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3711)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3711
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30105_length_3711_cov_3.487144"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..315)
                     /locus_tag="PLP95_07060"
     CDS             complement(<1..315)
                     /locus_tag="PLP95_07060"
                     /inference="COORDINATES: protein motif:HMM:NF013607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB45596.1"
                     /translation="MSDSPRPTEDRPTEDRPTVLFLCIHNAGRSQMALGWFRHLAAGR
                     ATAWSGGSEPAEQLNPVAVAAMAEVGIDIAAEQPRRWTDANLRSAEVVITMGCGDTCT
                     YYP"
     gene            complement(312..1079)
                     /locus_tag="PLP95_07065"
     CDS             complement(312..1079)
                     /locus_tag="PLP95_07065"
                     /inference="COORDINATES: protein motif:HMM:NF012455.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MIP/aquaporin family protein"
                     /protein_id="HPB45597.1"
                     /translation="MRRSPDTLQPTPLQRDTLRPGALSGGRAAVAEALGTGLLVAVVV
                     GSGIAAQRLSPDDLGLQLLQNSLATGAGLAALIAVFEPVSGAHFNPVVTLIDWFGGAI
                     RSATATAYIAAQILGAGLGCMIANLMFDLDAVAVSHTERSSSGVWLGEVVATFGLMMV
                     ILGLGRTGRARFAPFAVGGYITAAYWFTSSTSFANPAVTLGRTLTDTFTGIAPSSAAP
                     FIAFQLIGGTLAFALSRYLHPSTHPSTERPTTPGAAQ"
     gene            complement(1076..1528)
                     /locus_tag="PLP95_07070"
     CDS             complement(1076..1528)
                     /locus_tag="PLP95_07070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsI/CadI family heavy metal resistance
                     metalloenzyme"
                     /protein_id="HPB45598.1"
                     /translation="MSRIQLALNVSDIDQAVEFYSKLFAAEPAKRKPGYANFAIADPP
                     LKLVLIEGAVGGTLNHLGVEVDTAPEVEAAESRLSGDGLDTTGVDDTTCCYAHKTETW
                     VTDPDGAPWEFYVKTGDAEQMTNSQRAGAGADTGAASASGAASAISCC"
     gene            1607..1948
                     /locus_tag="PLP95_07075"
     CDS             1607..1948
                     /locus_tag="PLP95_07075"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HPB45599.1"
                     /translation="MKKIATVDECCPQVLNAPLDADEADLLAAGFKALADPVRLRLLS
                     LIANAPGGTACSCDLEEPVGKSQPTVSHHLSLLADAGLITKEKVGRWVNCTIVSERIE
                     ALRDALDSRTP"
     gene            2122..>3711
                     /locus_tag="PLP95_07080"
     CDS             2122..>3711
                     /locus_tag="PLP95_07080"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HPB45600.1"
                     /translation="MSLSVEHLAYAHPGGDILFDDVSFKCPRGVVSALVGGNGVGKTT
                     LMRLVAGELDALDGDIAIAGEVHYLPQDLGIGDTDVTVKELLTAVAPKHLAAAGKQLI
                     EVESRLIAGDDQAAFEIGDALDAWGGAGGYQLEATWDEACRRVLGDGLSAVGDRMVST
                     LSGGERKRLVLDVVFALDPEVVLLDEPDNFLDIPAKNWLGECLQQCDATVLLISHDRE
                     LLSTAADRIVTLEASGAWVHGASYRTYDDARAERQERLAADVARWEEEERRLFQFYKT
                     MKQRAASNHRNAARADAAETRWKKYVEAGPPPPPAPRHQITPKLVGADSGRVVLRCDG
                     LGVDGLVLPFSGEIHFGDRVGLVGPNGTGKSHLMRTFAGELAAGDGAFTYGARINMGY
                     FSQMNRRVEFDDSELFEIARRRVTNDTAAMGALGRYGLAGASDRLVDTLSGGQRARLD
                     VMCLELDGANLLLLDEPTDNLDLESSEVLQDALSAFTGTVIAVSHDRAFLRSLSTFWH
                     IDADGIVRVNAVSASCNTSEDS"
BASE COUNT          584 a         1104 c         1383 g          640 t
ORIGIN      
        1 ggggtaatag gtgcaggtgt ccccgcaccc catggtgatg acgacctcgg cgctgcgcag
       61 gttggcgtcg gtccagcgtc gtggctgttc ggcggcgatg tcgatgccga cctcggccat
      121 ggcggccacg gcgacggggt tgagctgctc ggccggttcg ctgcctccag accaggccgt
      181 cgctcgacct gcggcaaggt gacggaacca tccgagcgcc atctgtgacc gaccggcgtt
      241 gtgtatgcac agaaacagta cggtcgggcg gtcttcggtc ggacggtcct cggtcgggcg
      301 gggggaatcg ctcactgggc tgctcctggt gtggtgggtc gttcggtgga ggggtgggtg
      361 ctggggtgga ggtagcggga tagggcgaag gcgagggtgc cgccgatcag ttggaacgcg
      421 atgaagggcg ctgcgctcga cggtgcgatt ccggtgaagg tgtcggtcag ggtccgcccg
      481 agagtgactg cggggttggc gaagctcgtt gaggaggtga accagtaggc ggcggtgatg
      541 tagccgccga ccgcgaacgg cgcgaacctc gctcgtccgg tgcgaccgag tccgaggatg
      601 accatcatga gcccgaacgt ggcgaccacc tcgccgagcc atactcccga cgatgaccgc
      661 tccgtgtggg agacggcgac cgcgtcgagg tcgaacatga ggttggcgat catgcagccg
      721 aggccggcac cgaggatctg ggcggcgatg taggcggtgg cggtcgcgga tcggatggcg
      781 ccgccgaacc agtcgatgag ggtgacgacc gggttgaagt gggcgcccga gaccggttcg
      841 aacacggcga tgagagcggc gaggcctgca ccggttgcga gcgagttctg caggagttgc
      901 aggccgaggt cgtcggggga gagccgctga gcggcgatgc cggatccgac gacgacggcc
      961 acgagcagcc cggtgccgag cgcctcggcg accgcggccc ggcccccgga cagcgcgccg
     1021 ggccgcagcg tgtcgcgctg cagcggggtg ggctgcagcg tgtcgggcga gcgtctcaac
     1081 agcagctgat ggccgaggcg gcgcccgagg ctgaggcggc gccagtgtcg gcaccggcgc
     1141 cggcgcgctg cgagttggtc atctgctcgg cgtcgccggt cttgacgtag aactcccagg
     1201 gggcgccgtc ggggtcggtg acccacgttt cggtcttgtg ggcgtagcaa caggtcgtgt
     1261 cgtcgacgcc ggtggtgtcg agcccgtcgc cggacaagcg ggattcggcc gcctcaacct
     1321 cgggtgcggt gtcgacttcg acgccgaggt ggttgagggt gccacccacg gcgccttcga
     1381 tgaggacgag cttgagtggc gggtcggcga tggcgaagtt ggcgtatccg ggtttgcgct
     1441 tggcgggctc cgcggcgaac agcttggagt agaactcgac ggcctggtcg atatcggaca
     1501 cgttgagggc gagttggatt cgtgacatcg ggattctcct cgggttgcgg taatctattg
     1561 acgaatatca atgtatcgat gggtgtcgat gaatgcaagg agtgtcgtga agaagatcgc
     1621 cacggtcgac gagtgctgtc ctcaggtcct caacgcaccg ctcgacgcag acgaagccga
     1681 cctgctcgcc gcgggattca aggcgttggc ggatccggtg cgcttgcggc tgctgtcgct
     1741 catcgccaac gctcccggcg ggaccgcctg ttcgtgcgac ctcgaagagc cggtcgggaa
     1801 gtcgcagccg acggtgtcgc atcatctgtc attgctcgcc gacgccgggt tgatcaccaa
     1861 ggagaaggtc ggccgctggg tcaactgcac gatcgtttcc gagcgaatcg aagcgctgcg
     1921 cgacgcactc gactcccgaa cgccctgaac caagccgaag ccgagcggcg tgtccggcgc
     1981 ccacagttga actgtgggcg attccggcca cctgtgcgcc tgatttgccc acagttgaac
     2041 tgtgggtcga accgccagtc gaaccgccgg tcgaaccgcc agccgaaccg ccagccgaac
     2101 cgccttggct agtgtgccga cgtgtcgttg agcgtcgaac atctcgccta tgcccatccc
     2161 ggtggggaca tcttgttcga cgatgtgtcg ttcaagtgcc cgcggggtgt ggtgagcgcg
     2221 ctggtcggcg gtaacggtgt cggcaagacg acgttgatgc gtctcgtcgc cggcgagttg
     2281 gacgcgctcg acggcgacat cgcgatcgct ggcgaggttc actatctgcc acaggacctc
     2341 ggcatcggcg acaccgacgt cacggtcaag gagttgttga cagcggtcgc cccgaagcat
     2401 ctggccgccg ccggcaagca gttgatcgag gttgagtcgc gcctgatcgc cggcgacgac
     2461 caggccgcgt tcgagatcgg cgacgcgctc gacgcctggg gtggtgccgg cgggtaccag
     2521 ctcgaggcca cctgggatga ggcgtgtcgg cgggtgctcg gcgacgggct gagcgcggtc
     2581 ggcgaccgga tggtgagcac gctgtcgggt ggcgagcgca agcgcctggt gctcgacgtc
     2641 gtgttcgccc tcgaccccga agtggtgttg ttggatgagc cggacaactt cttggacatc
     2701 ccggcgaaga actggctcgg cgagtgcctg caacagtgtg acgccacagt gttgttgatc
     2761 tcccacgacc gtgagttgtt gtcgaccgct gcggatcgaa tcgtcaccct ggaggcctcg
     2821 ggtgcgtggg ttcacggagc cagttatcgc acctatgacg atgcgcgggc cgaacgccaa
     2881 gaacgcctcg ccgccgatgt cgcccggtgg gaggaagagg agcgtcgcct ctttcagttc
     2941 tataagacga tgaagcagcg tgcggcgagc aatcaccgca atgcggcgcg ggccgacgcc
     3001 gcggagacgc ggtggaagaa atacgtcgaa gcgggcccgc cgccgccccc ggcgccgcgc
     3061 catcagatca cccccaagct ggtcggggcc gacagcgggc gggtggtgtt gcgctgcgac
     3121 ggcctcgggg tggatggatt ggtgttgccg ttcagcggcg agatccactt cggcgaccgg
     3181 gtgggtctgg tcgggccgaa cggaaccggt aagtcgcatc tgatgcggac cttcgccggc
     3241 gagctcgcgg ctggcgacgg ggcgttcacc tacggcgccc gcatcaacat ggggtacttc
     3301 tcgcagatga accggcgcgt cgagttcgac gacagcgagt tgttcgagat cgcccgtcgt
     3361 cgggtcacca acgacaccgc ggcgatgggt gcgctcgggc gctacgggtt ggcgggagct
     3421 tcggaccggt tggtcgacac gctgtcgggt ggccaacgcg cccggctcga cgtcatgtgc
     3481 ctcgaactcg acggtgcgaa cctgttgctg ttggacgagc cgaccgacaa cttggatctc
     3541 gagtcctccg aggtgttgca ggacgcgctc tcagcgttca ccggcacggt catcgcggtg
     3601 tcccacgacc gggcgtttct gcggtcgctg tcgacgtttt ggcatatcga cgccgacggg
     3661 atcgtgcggg tgaacgctgt gagcgcgtcc tgcaacacct cggaggactc g
//
LOCUS       DAOOMA010000226         3693 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_30284_length_3693_cov_4.276251, whole genome shotgun sequence.
ACCESSION   DAOOMA010000226 DAOOMA010000000
VERSION     DAOOMA010000226.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 3693)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3693)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3693
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30284_length_3693_cov_4.276251"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            53..2047
                     /locus_tag="PLP95_07085"
                     /pseudo
     CDS             53..2047
                     /locus_tag="PLP95_07085"
                     /inference="COORDINATES: protein motif:HMM:NF014961.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 dependent-methionine synthase
                     activation domain-containing protein"
     gene            2110..>3693
                     /locus_tag="PLP95_07090"
     CDS             2110..>3693
                     /locus_tag="PLP95_07090"
                     /inference="COORDINATES: protein motif:HMM:NF012770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HPB45601.1"
                     /translation="MSMLRLRRGLIAGVVACAIVAGCAGRDEGDDGHRAAGTPSTTTS
                     APVPDALSRESFAPYEPLWTARDCGDTGSAASPIDPDIEVSCGTLTVPENRREPQGNL
                     VELAVRRIGFVGEGAAPDPVVYLHGGPGSGVYQSGSARWERLAHELRREVIVFDQRGV
                     GASSPNLNCPEREDAFIEVLSGTAGWDVELANIQTALSTCFERLNAAGHDLSQYNTFA
                     NAADLADLRVALGIDAWNLWGVSYGTRLALTALRFFPEGIRSVVLDSVSPPSAANVYG
                     SLVSGREAFDRLANGCGTEPACNERYGDIRPAIDAIYAAMNEQPAETDLTVGDQNLHL
                     RLDGSDAIAGLFNAMYDTTLIPQLPAAITDLAAGGRGIVAMIAQVGIPFINSMTEGAY
                     LSYECADNTARIEPSEVDSLIEDPGEAATLLLAGWNLFCEQWPIEPLPVEFGDVVTRD
                     LVPDDVAVIVFAGEYDPITPSNRSREVAEAIDALAFVEIPRGGHGAQSSNSCTKGLFL
                     RFINDPTQLDTTCVASIPVV"
BASE COUNT          665 a         1253 c         1143 g          632 t
ORIGIN      
        1 catcgccatc aaccgctacg ggctggagcc gggcgacctc atcttcgatg ccttgacctt
       61 cccgctgtcc accggcgacg aagacctgcg aaacgacgcg gccgccacca tcgaggccat
      121 ccgccgaatc cacgccgagc ttccgggcgt gtacaccacc ctcgggctgt cgaacgtgag
      181 cttcgggctg tcccccgcga gccgccacgt gctcaactcg gtgttcctcg ccgagtgcat
      241 caaggccgga ctcacctctg ccatcgcgca cgcgtcgaag atcattcccc tgtcccgcat
      301 ccccgatgaa cagcgcgacg tgtgcctcga tctcatctgg gaccggcggg gcaccgcagg
      361 agcgttgtcg aacggcgacc cgaactacga cccacttcac cggctcctgg agatcttcgc
      421 cgacgtacag gtggaggccg tcgtcaagga ggaccgctcc gactggccgg ttgagcggcg
      481 cctgtcgacg cgcatcatcg acggcgattc cgaaggcctc accgacgatc tcgacgaagc
      541 gatcgccgga ggcctcgccc ccctggccat catcaatgat gtcctgttgg aggggatgaa
      601 ggtcgtcggt gacctcttcg gcaaggggga gatgcaactg cccttcgtgc tgcaatcggc
      661 cgagacgatg aagcgggcag tcgcctatct ggagccgatg atggacagct ccgaggcggg
      721 cgcgggcaag ggcagggtcg tgttggccac cgtgaagggc gacgttcacg acatcggcaa
      781 gaacctggtc gacatcatcc tcaccaacaa cggctacgag gtgcacaacc tcggcatcaa
      841 ggtgtcgatc gccgagatga tcgacaaggc gctcgaagtc gaggccgacg cgatcggcat
      901 gagcggcctg ctggtgaagt cgacgctcat catgcgcgac aacctcgagg agctcaacgc
      961 ccgcaacctg tcgaagatcc ccgtgatgct cggcggcgcg gcactcaccc gcacctacgt
     1021 cgagcgcgac cttcgcgagg tctacacggg ccgccttttc tacggaaagg atgcgttcga
     1081 gggcctccat gtcatggaca agctcggtca gatcaaggcc ggcaccgtgg tcgacgacgc
     1141 ctggggggtc gagccgggag gtcgagccct gcccaagcgc gagaaagttg aaatcgaccc
     1201 cgccaccatc cctgcccgct cccccgaggt ggccgcagac aacccggtgt tcgcgccgcc
     1261 cttccttggg agtcgcatcg tgaaggggtt ggcgatcgac gacattgcgc ccttcatcaa
     1321 cgagacgacg atctttcgca accagtggca gttccggccc gagaacggcg aaaccgacga
     1381 gcagttcaaa gagcgactgc gtcccgagct tcgcgcccga ctggcacagt gcaaggccga
     1441 ggaactgctc gttccccagg tcgtctacgg atacttctgc gccaactcca tcggcgacga
     1501 cctcgtcatc tggtccgatg agacgcgcac cgaagagttg ggccggttcc actatccccg
     1561 ttagtaggtg gagccgtggc tgtgcatcgc ggacttcttt cgtctctacg acgacggggc
     1621 cggcgaactc gactacgccg cgttccacat cgtcaccatg gggccgcgca tctctgagta
     1681 ctgcgcgcag ctgttcgccg acaatcgcta caacgactac ctgcggtttc acggaatcgg
     1741 tgtggagatg gccgaggccc tcgccgagta ctggcacaaa cgcatccgaa ccgaatgggg
     1801 attcgtcgac gaggacggac cgaccacgat cggacttttc cgccagcagt accgtggcgg
     1861 gcgctactcg tccggatacc cggcgtgccc gaatctcgag gacaatgcgc tcatcaacga
     1921 cctgctcgac ggcgggcgca tcggccttga ggtgtcggag gagaccggct ggcaatacca
     1981 ccccgaacag agcacctcgg cgctcatctg ccaccatcca cgggcgaagt acttcgtcgc
     2041 tcggtgacgc cgggcgccct ccgacccctc aagaggttgc gatgagcagg ttccgatgag
     2101 caggttccga tgagcatgct tcgactccgg cgcggtttga tcgccggagt cgtcgcgtgc
     2161 gcgatcgtcg cgggatgcgc tggccgagac gaaggtgacg acggacaccg agccgccggc
     2221 accccgtcga cgacaacctc agcgccggtt cccgacgcct tgtcccgcga gtccttcgcg
     2281 ccctacgaac cgctgtggac cgcacgggac tgcggcgata cgggttcggc cgcgtctccg
     2341 atcgatccgg acatcgaagt gtcctgtggg acgctcaccg ttccggagaa ccgacgtgag
     2401 ccacagggca acctcgtcga gttggccgtg cgcagaatcg gctttgtcgg cgagggtgct
     2461 gcccccgacc ccgtggtgta tctccatggc gggccgggca gtggcgtgta ccaatccggc
     2521 tccgcccggt gggaacgttt ggctcacgaa cttcggcgcg aggtgatcgt cttcgaccaa
     2581 cgcggcgtcg gggcgtcgtc gccgaacctc aactgccccg aacgcgagga cgcgttcatc
     2641 gaagtgctct ctggcaccgc cggatgggac gtcgaactgg cgaacatcca gaccgcgctt
     2701 tccacgtgct tcgaacggct gaacgccgcc ggacacgacc tgagtcagta caacacgttc
     2761 gccaacgccg ccgacctggc cgacctgcgc gtcgcgctcg gaatcgacgc atggaacctc
     2821 tggggtgtgt cctatgggac tcgtctcgcg ctcaccgccc tgcgcttctt ccccgagggc
     2881 atccgttcgg tcgtactcga ctcggtttct ccgccgagtg ccgcgaacgt ctacggatcg
     2941 ttggtatctg ggcgagaggc cttcgacagg ctcgcgaacg gctgcggcac cgaacccgcg
     3001 tgcaatgagc gctatggcga catccgcccc gctatcgacg ccatctacgc ggcgatgaac
     3061 gaacagcccg ccgaaaccga cctcacggtt ggcgaccaga acctgcacct gcgtctcgac
     3121 ggatccgatg cgatcgccgg gctgttcaac gcgatgtatg acaccacgct gatcccgcag
     3181 ttgccagcgg cgatcaccga tctcgcagcg ggcggccggg gcatcgtggc gatgatcgcc
     3241 caggtcggca ttccgttcat caactcgatg accgagggcg cctacctgtc ctacgaatgc
     3301 gccgacaaca ccgctcggat cgagccttct gaggtcgact cgctcatcga ggatcccggc
     3361 gaggcggcga cgttgctgtt ggccggctgg aacctgttct gcgaacagtg gccgatcgaa
     3421 ccgttgcccg tcgaattcgg cgacgtcgtc acccgtgacc tggtaccgga tgacgtcgcg
     3481 gtcatcgtgt tcgccgggga atacgacccg atcactccga gcaatcgttc ccgtgaggtt
     3541 gccgaagcca tcgatgccct ggcgttcgtc gagatccccc gaggcggcca cggggcgcag
     3601 tcgtccaact cgtgcaccaa gggcctcttc ctgcgattca tcaacgaccc gacacagctc
     3661 gacaccacct gcgtcgcgtc gattcccgtc gtt
//
LOCUS       DAOOMA010000227         3673 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_30513_length_3673_cov_3.243781, whole genome shotgun sequence.
ACCESSION   DAOOMA010000227 DAOOMA010000000
VERSION     DAOOMA010000227.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 3673)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3673)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3673
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30513_length_3673_cov_3.243781"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..394)
                     /locus_tag="PLP95_07095"
     CDS             complement(<1..394)
                     /locus_tag="PLP95_07095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB45602.1"
                     /translation="MGNTTRATATAWLSAFAAVVAIVASGCSAGQQAGADEVGFGDGA
                     SPGTTAGHGVVRIGHVGELVSDPLAVAATDSASVMLVDALTDGLTSVDDDGSVVPAVA
                     SSWDSADGATWRFTIDPTRTWSDGTPISA"
     gene            complement(566..796)
                     /gene="secG"
                     /locus_tag="PLP95_07100"
     CDS             complement(566..796)
                     /gene="secG"
                     /locus_tag="PLP95_07100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012231023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HPB45603.1"
                     /translation="MYLVSLVLQFVLSIAVIVLVLIHSGKGGGLSDMFGGGLSAAASG
                     STAMERQLTRITVVVALLFSFNTVILALLMDK"
     gene            complement(869..1261)
                     /locus_tag="PLP95_07105"
     CDS             complement(869..1261)
                     /locus_tag="PLP95_07105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HPB45604.1"
                     /translation="MARAWDDARHDELLNGVRGICMSFPEVSERLSHEMPTWFVREKR
                     VVAYFVADHHDDGYLGFMCPAPDGVQAELIEAEPDRFYRPPYFGHRGWIGVHLAEDCD
                     WDEVREILLDAYRKVAPKVLAAQLEAGK"
     gene            complement(1405..2175)
                     /gene="tpiA"
                     /locus_tag="PLP95_07110"
     CDS             complement(1405..2175)
                     /gene="tpiA"
                     /locus_tag="PLP95_07110"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003498915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HPB45605.1"
                     /translation="MPKRIPIISGNWKMNLNHLEALTLVQKLSYNLAGHNYDAVEVTV
                     HPPFVDIRTVQTAIESDRMSIVLGAQNCYFEEKGAFTGEVAPSMLAKLNVSYVILGHS
                     ERREIFGETDEMVNKKVHAVFKAGMTPILCCGETQAEREGGEAEAKVGGQIRAGLAKL
                     STEQVASMVIAYEPIWAIGTGLTATPDDAQAMCKHVRTVVAEVAGQAAADAVRIQYGG
                     SVKGGNASELMAQPDIDGALVGGAALDADDFARIVQFR"
     gene            complement(2168..3307)
                     /locus_tag="PLP95_07115"
     CDS             complement(2168..3307)
                     /locus_tag="PLP95_07115"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020376887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HPB45606.1"
                     /translation="MKFGVPELEDLPDVSGKSVLVRVDFNVPIKDDDGSKTITDDLRI
                     RAALPTLAWLTERGAKVTCVTHLGRPKGAPDPAFSVEPVRARLAELAPGVELLENVRF
                     NAGETANDPAFVQELIAGHDLYVNDAFGASHRAHASIVGPPQFLPSAAGRLLAKEVEV
                     LGGLRNDPARPFIAITGGSKVSDKLGVIEAMLDIADGLIVGGGMCFTFLAALGHSIGN
                     SLFEAEQVDNCKRLLDAHADRLHLPTDIVVTNGKLFDPEAGGDVRHVGRNVPDGYMGV
                     DIGPESAGNFAEIILGARTVLWNGPMGVFEDPRFEAGTKAVAEAVAETRGFSVIGGGD
                     SAAAIAQFGLAADVDHVSTGGGASLELIEKGDLPGLAALRGASNA"
     gene            complement(3309..>3673)
                     /locus_tag="PLP95_07120"
     CDS             complement(3309..>3673)
                     /locus_tag="PLP95_07120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012231018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HPB45607.1"
                     /translation="SLVLESMKGRLDGTSLRVPIPTGSITDFTGLLNTEVTVDEVNAA
                     FKAAAESGPLVGILQYTDEPIVSSDIVGSTYSSIFDSGLTMAMGNMVKVLSWYDNESG
                     YSNRLVDLALKVGAANQG"
BASE COUNT          669 a         1286 c         1134 g          584 t
ORIGIN      
        1 gtgcggagat cggtgtcccg tctgaccacg tccgcgtcgg gtcgatcgtg aagcgccacg
       61 tcgccccatc ggccgaatcc cacgaactcg cgaccgccgg caccaccgac ccgtcgtcgt
      121 ccaccgaggt caggccgtcg gtcagcgcgt cgaccagcat caccgaggcc gaatcggtcg
      181 ccgccaccgc aagcggatcg ctcaccaact ccccgacgtg gccgatccga acgaccccat
      241 gtcccgcggt cgtcccgggc gaggcaccgt caccgaaacc aacctcatcg gcacccgcct
      301 gctgcccggc cgagcacccc gacgccacga tcgccaccac tgcagcgaac gccgagagcc
      361 atgcggtagc ggttgccctc gtggtgttcc ccatgacggg aagtgtcggc accgatgctc
      421 atcagctgta gaaccggcga aacccccgca cccgcacgcg aagattctgc tgaaagcgac
      481 accacgtgtc gctttcagca gaaagtttga cgctagccgc cgaacggcag ccgcgagcca
      541 acggcgagac gccggccgcg gcggactact tgtccatcaa caacgcgagg atgaccgtgt
      601 tgaacgagaa cagcagcgcc acgacaaccg tgatgcgcgt gagctgacgt tccatcgccg
      661 tcgaacccga ggccgcggcg ctcaggccac cgccgaacat gtcggacaga ccgccaccct
      721 taccgctgtg gatcaacacg agcacgatga ccgcgatcga caacacgaac tggaggacca
      781 acgacacgag gtacatagcg tcctacacct tactcgcact ggcccccgta gcggctactc
      841 ccccgctact cccccgctac tcccccggct acttcccggc ttcgagttgc gcagcgagca
      901 ccttcggggc gaccttgcgg tacgcgtcga gcaggatctc gcggacctcg tcccagtcgc
      961 agtcctccgc gagatgcacc ccgatccacc cgcgatggcc gaagtagggc ggacggtaga
     1021 accggtccgg ttccgcctcg atcagttcgg cctgcacgcc atcgggagcg ggacacatga
     1081 aacccagata cccgtcgtcg tgatgatcgg caacgaagta cgccacaacc cgcttctcgc
     1141 gcacgaacca cgtgggcatc tcgtgactca gccgctcgct gacctcggga aacgacatgc
     1201 agatcccccg aacgccgttg agcaactcgt cgtgtcgtgc gtcgtcccaa gcccttgcca
     1261 tccccccgac gctacccgaa gcaacccgcc gcgattgctc gacacgccgc tacccgcagc
     1321 aacccgccct caagaacccg aaacgcccgg tgtcgcggct gcggtcacag ccgaaacacc
     1381 gggcgtccca gaaaatacaa acgatcagcg gaactgcacg atgcgggcga agtcgtccgc
     1441 atcgagtgcg gcgccgccga cgagcgcacc gtcgatgtcg ggctgcgcca tcagctccga
     1501 cgcattaccg cccttcaccg agccgccgta ctggatccgt accgcgtcgg ccgctgcctg
     1561 cccggccacc tcggccacca ccgtgcggac gtgcttgcac atggcctgcg catcgtcggg
     1621 ggtggcggtc aaaccggtgc cgatcgccca gatcggctcg taggcgatga ccatcgaggc
     1681 cacctgttcg gtgctcagct tcgcgagacc ggcacggatc tggccgccga ccttcgcctc
     1741 agcctcgccg ccttcgcgtt cggcctgggt ctcgccgcag cacaggatcg gcgtcatccc
     1801 cgccttgaac acggcgtgca ccttcttgtt caccatctcg tcggtctcgc cgaagatctc
     1861 gcgacgttcc gagtggccga ggatcacata gctgacgttc agcttcgcca acatcgacgg
     1921 tgcgacctca ccggtgaagg cgcccttttc ctcgaagtag cagttctgcg cacccagcac
     1981 gatcgacatg cgatccgact cgatggcggt ctgcacggtc cggatgtcga caaacggcgg
     2041 gtgaaccgtc acctcgacgg cgtcgtagtt gtgacccgca aggttgtagc tgagcttctg
     2101 gacgagcgtg agggcctcaa ggtgattgag gttcatcttc cagttgccgc tgatgatcgg
     2161 gatgcgctta ggcattgctt gctcctctga gggccgcgag gccgggaagg tcgccctttt
     2221 cgatgagttc cagcgaggcg ccgccgccgg tcgacacgtg gtcgacgtcg gccgcaagtc
     2281 cgaactgggc gatggccgcg gcactgtcgc cgccgccgat cacgctgaat cccctggttt
     2341 cggccaccgc ctcggcgacc gccttggtgc cggcctcgaa acgcgggtcc tcgaacaccc
     2401 ccatcgggcc gttccacaac acggtgcgag caccgaggat gatctcggcg aagttgccgg
     2461 ccgattcggg gccgatgtcg acgcccatgt agccatcggg aacgttgcgc ccgacgtggc
     2521 gaacgtcgcc gcccgcctcc gggtcgaaca atttgccgtt ggtgaccacg atgtcggtcg
     2581 gcagatgcag ccgatcggcg tgggcgtcga gcaggcgctt gcagttgtcg acctgttcgg
     2641 cctcgaacag cgaattgccg atcgaatggc cgagcgcagc caggaacgtg aaacacatgc
     2701 ccccgccgac gatcagacca tccgcgatgt cgagcatcgc ctcgatcaca cccaacttgt
     2761 ccgacacctt cgacccgccg gtgatggcga tgaaggggcg cgccgggtcg ttgcgcaacc
     2821 cgccgagcac ctcgacctcc ttggccaaca ggcgacccgc ggcggaggga aggaactgcg
     2881 gcggaccgac gatggacgcg tgcgcacggt gcgaggcacc gaacgcgtcg ttgacgtaca
     2941 ggtcgtgacc ggcgatgagt tcctgcacga aggcagggtc gttcgcggtc tcgccggcgt
     3001 tgaagcggac gttttccaac agctccacgc cgggggcgag ttccgcgagg cgggcccgaa
     3061 ccggctcaac gctgaacgcc ggatcgggcg cgcccttggg gcgtcccagg tgggtgacac
     3121 aggtgacctt cgcaccgcgc tcggtcaacc acgcgagggt gggcaacgcg gcgcggatgc
     3181 gcaggtcatc ggtgatggtc ttcgagccgt catcgtcctt gatgggcacg ttgaaatcaa
     3241 cgcgcaccag gaccgactta cccgagacgt cgggcaggtc ctcgagttct ggaacgccga
     3301 acttcatctc agccctggtt ggccgcgccg accttgagcg ccaggtcgac gagacggttc
     3361 gagtagcccg actcgttgtc gtaccacgac agcaccttga ccatgttgcc catcgccatg
     3421 gtgaggcccg agtcgaagat cgagctgtag gtggaaccca cgatgtcgga gctgacgatc
     3481 ggctcgtcgg tgtactgcaa gatgcccacg agcgggcccg actcggcggc ggccttgaat
     3541 gcggcgttca cttcgtcgac ggtgacctcg gtgttgagca ggccggtgaa gtcggtgatc
     3601 gaaccggtgg ggatcggcac acgcaacgag gtgccatcga ggcggccctt catcgactcc
     3661 aacacgagcg acg
//
LOCUS       DAOOMA010000228         3670 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_30547_length_3670_cov_3.276902, whole genome shotgun sequence.
ACCESSION   DAOOMA010000228 DAOOMA010000000
VERSION     DAOOMA010000228.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 3670)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3670)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3670
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30547_length_3670_cov_3.276902"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1413)
                     /locus_tag="PLP95_07125"
     CDS             complement(<1..1413)
                     /locus_tag="PLP95_07125"
                     /inference="COORDINATES: protein motif:HMM:NF024850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HPB45608.1"
                     /translation="MKLRVVAALAISALLATACAAEGADEAVTGNTESTVAPSEGTDT
                     PDAGGAGRFGDIESPCGPGEATVAEADAGLGTDKLYLGVANNREGFKPGLLKELWDSS
                     TAFVQWCNAQGGIAGIPLEAVDLGVDVMAVEDGMAEACTDVFAMVGGGYAMDNLMFSG
                     KPGSDFHECGLIAFPGFAVSTDFSEATDQVQAIPNPAHLKAYGSYVQLADVYPDLIGD
                     FGVAFGDLASIEQNKDQVVGQAKLVEGFGNFSTVRYAVVNADYSVTAQQVIQAGIKLL
                     SFVGEPVSYAGLSQALNDQGWEGVTTADANQYDSRLITAQGPEAVEGAVIRLAAHPFE
                     EEDKWPATKQLVEILDEYSPDWEHAGLTIQSFSANLLFATAANECAKKNGEITRVCVL
                     QEGKAIKSWTGGGLHAEANPGDNEPSGCSVLVTIKDGKFERLHPEIGSEMDSEDGFYC
                     DELVTLEGNFGAGATSRSILD"
     gene            1620..3350
                     /locus_tag="PLP95_07130"
     CDS             1620..3350
                     /locus_tag="PLP95_07130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012222581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HPB45609.1"
                     /translation="MFDRLISNATIVDGTGADRRSGSVAIQDGRIAAIFDAGDELPEA
                     SEVIDGTGLVLTPGFIDPHTHYDAQLMWDPAASPSNLHGITTILAGNCGFTLAPLKAA
                     DADYTRRMMAKVEGMPLKALEEGVDWNWSTFGEYLDRLDGNIAVNAGFLVGHCALRRH
                     VMGEEAVGNEATPEQLNEMIELLRTSIEAGGLGFSTTLSRTHTDGDGQPVASRWASRE
                     ELMAFAEVVSQFEGTTLEYASNGCLDGFDEDDIEFMIDFSLTAQRPLNWNVLTVDSNA
                     PERYRTQLEAMDRCREAGAKVVALTMPILVGMNMSFLNFCGLNLLPDWGDILGLPLDE
                     RMEKLRDPEVRRFMVERAASPDAGVFARLAGWDGYVIGQTYSEANAGLRGKTAGQIGA
                     ERGISAFDALLDVVLADDLRTVLWPGPTDDDDLSWQMRAEAWEHPSVIIGGSDAGAHL
                     DRMAGPAYTTQWIGDCLRGRKLTTLERAVQHLTEAPAKLFGLKDRGLIAAGYAADLVM
                     FDPETIGAGKIELVDDLPGGTARLVPEAKGIKRVIVAGTDIVVDGTATDATPGRIMRP
                     GVATETVPLR"
     gene            complement(3307..>3670)
                     /locus_tag="PLP95_07135"
     CDS             complement(3307..>3670)
                     /locus_tag="PLP95_07135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB45610.1"
                     /translation="HLHHRLMRLGHGHRQTVIIMWSWTGLFSAFALYPAMSGRSTLIM
                     PIALAALLLLVFTLVFPWASQRRRRPNGDDAADGDDAADGDDAADAADAADGDDAADG
                     DDGGVSAAEPSRSPHPGA"
BASE COUNT          633 a         1239 c         1177 g          621 t
ORIGIN      
        1 gtcgagaatc gaccgtgatg tcgctcctgc gccgaagttg ccttcgagcg tcacgagttc
       61 gtcgcagtag aagccgtcct cgctgtccat ttcgctgccg atctcggggt ggaggcgttc
      121 gaactttccg tccttgatcg tgacgaggac cgaacagccg ctcggctcgt tgtcgccggg
      181 gttggcctcg gcgtgcagac cgccgccggt ccagctcttg atggccttgc cttcctgaag
      241 cacgcacacc cgggtgatct cgccgttctt cttggcgcat tcgttggcag cggtcgcgaa
      301 gagcaggttg gccgagaacg actgaatcgt caggccggca tgttcccaat cgggtgagta
      361 ctcatcgagg atctcgacga gttgcttggt cgccggccac ttgtcctcct cctcgaaggg
      421 gtgggcggcg aggcgaatca cggcaccctc gacggcctcg gggccctggg cggtgatgag
      481 gcgcgagtcg tactgatttg cgtcggcggt ggtgacgcct tcccaaccct ggtcgttgag
      541 cgcctgggac agcccggcgt aggagacggg ctcgccgacg aagctcagca acttgatacc
      601 ggcctggatg acctgctggg cggtgacgga gtagtcggcg ttgaccaccg cgtaccggac
      661 cgtgctgaag ttgccgaagc cctcgaccag cttggcctga ccgacgacct ggtccttgtt
      721 ctgctcgatg gacgccagat cgccgaacgc cacgccgaag tcgccgatca ggtcgggata
      781 cacgtcggcg agctgcacat aggagccata ggccttgagg tgcgccggat tggggatcgc
      841 ctggacctga tcggtcgcct cggagaagtc ggttgatacg gcaaaccccg ggaatgcgat
      901 cagaccgcac tcgtgaaagt cgctgcccgg cttgcccgag aacatcaggt tgtccatggc
      961 gtatccgcca ccgaccatgg cgaacacgtc ggtgcacgcc tcggccatgc cgtcttccac
     1021 cgccatgaca tcgacgccga gatcgacggc ctcgagcggg atgccggcga taccgccctg
     1081 ggcgttgcac cactgcacga acgccgtgct ggagtcccag agttccttga gaaggccggg
     1141 cttgaagccc tcgcggttgt tggcgacgcc gaggtacaac ttgtcggtgc ccaagcccgc
     1201 gtcggcttcg gcgacggtgg cttcgcccgg cccgcagggc gactcgatgt cgccgaatcg
     1261 acccgctccc ccggcatcgg gtgtatcggt gccttcggac ggcgcgacgg tcgattcggt
     1321 gttgccggtg acggcctcgt cggcgccttc agccgcacac gcggttgcca acagtgccga
     1381 gatcgccaag gcggcgacga ctcgcagttt catgcatatc ccccatgtcg atgagtggcg
     1441 gccatccaat caggtcggat gacgggccgc aaccttagtc agcaatctcg accttcggtt
     1501 cgacttgttt ctgacggacc gtcagatttt gataccgtcg ggttccaggt gccgcacgca
     1561 ccgctccgaa cggtctcaaa cgaccgcccg cacaacgaat gccgaacgag gtgttgaaca
     1621 tgtttgatcg actcatttcg aacgcgacga ttgtcgacgg caccggcgca gaccgccgaa
     1681 gcggatcggt cgccatccag gacgggcgca tcgctgcgat cttcgacgca ggcgacgaat
     1741 tgcccgaagc atccgaggtg atcgacggca ccggcctggt cctcaccccc ggattcatcg
     1801 acccccacac ccactacgac gcccaactca tgtgggaccc agcggcctcg ccgtccaacc
     1861 tgcacggcat caccacgatc ttggcgggca actgcggatt caccctcgcc ccgctcaagg
     1921 ccgccgatgc cgactacacc cgtcggatga tggccaaggt cgagggcatg ccgctcaagg
     1981 ccctcgagga gggggtcgac tggaactggt cgacctttgg cgagtacctc gaccggctcg
     2041 acggcaacat cgcggtgaac gccggcttcc tcgtcgggca ctgcgccctt cgccgccacg
     2101 tgatgggcga ggaggccgtc ggcaacgagg cgaccccgga acagctcaac gagatgatcg
     2161 aactgctgcg cacctcgatc gaggcgggcg gtctgggctt ttccaccacc ctgtcgcgca
     2221 cccacaccga cggcgacggc cagccggtcg ccagccgttg ggccagccga gaggaattga
     2281 tggcgttcgc cgaggtggtg tcgcagttcg agggaaccac gctggaatac gcatccaacg
     2341 gatgtctcga cgggttcgac gaggacgaca tcgagttcat gatcgacttc tccctcaccg
     2401 cccaacgacc gctcaactgg aacgtgctca ccgtcgactc caacgccccg gagcggtacc
     2461 gcacccaact cgaggccatg gatcgctgcc gcgaggcggg ggccaaggtc gtcgcgctga
     2521 ccatgccgat cctggtcggg atgaacatga gcttcctgaa cttctgtggc ctcaacctgt
     2581 tgcccgactg gggcgacatc ttgggcctgc ccctcgacga acgcatggag aagctgcgcg
     2641 atcccgaggt gcgccgcttc atggtcgagc gggcggcgtc gcccgacgcc ggagtgttcg
     2701 cccgcctggc cggctgggac ggatacgtca tcggccagac ctactccgag gccaacgccg
     2761 ggctgcgcgg aaagaccgcc ggccagatcg gtgccgaacg cggaatctcc gcattcgacg
     2821 cactgctcga cgtcgtgttg gccgacgacc tccgcaccgt gttgtggccc ggcccgaccg
     2881 acgacgacga cttgtcgtgg cagatgcgcg ccgaggcgtg ggaacacccg agcgtaatca
     2941 tcggcggttc cgacgccggc gcccacctcg accgcatggc tgggccggcc tacaccaccc
     3001 agtggatcgg cgactgcctg cgtgggcgca aactgacgac gctcgagcgg gccgttcagc
     3061 acctcaccga ggcgccggcg aagttgttcg gcctcaagga ccgcggactc atcgctgcgg
     3121 gctacgccgc cgacctcgtg atgttcgatc ccgagacgat cggcgccggc aagatcgaac
     3181 tcgtcgatga tctccccggc ggcaccgccc ggctcgtgcc cgaggccaag ggcatcaagc
     3241 gggtcatcgt cgcgggaacc gacatcgtcg tcgacgggac tgccaccgac gcgaccccgg
     3301 gtcggatcat gcgcccgggt gtggcgaccg agacggttcc gctgcgctga ccccaccgtc
     3361 atcaccgtct gcggcatcgt caccgtctgc ggcgtctgcg gcgtctgcag cgtcgtctcc
     3421 gtctgcagcg tcgtcaccgt ctgcggcgtc gtcaccattt ggtcgacgac gccgctgcga
     3481 ggcccacggg aacaccagcg tgaacaccag caacagcagc gccgccaagg cgatcggcat
     3541 gatcaatgtc gagcgacccg acatcgccgg gtagagcgcg aacgcgctga acagacccgt
     3601 ccagctccac atgatgatca ccgtctgacg gtgaccatga ccgaggcgca tcagccggtg
     3661 atgcaggtga
//
LOCUS       DAOOMA010000229         3656 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_30696_length_3656_cov_3.601777, whole genome shotgun sequence.
ACCESSION   DAOOMA010000229 DAOOMA010000000
VERSION     DAOOMA010000229.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 3656)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3656)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3656
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30696_length_3656_cov_3.601777"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(94..666)
                     /locus_tag="PLP95_07140"
     CDS             complement(94..666)
                     /locus_tag="PLP95_07140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB45611.1"
                     /translation="MKSKKLLVIGAGALAAAAVSGCVQEPIAVPDFTRVSCGSLGGGV
                     EFTPAVTEEVQAVQAAATEWTGSTDCDDETGSELTSLSMEGLAIEFPEMGCTTGAGTG
                     TATLRWSDESTSEVSLVVNLDKLSSGTASGNALVVVNTGRFTSWATVTPDAPDSSLST
                     PSAPNGGGALKRYSHTGRPASAMAAARMRT"
     gene            838..1869
                     /gene="ispH"
                     /locus_tag="PLP95_07145"
     CDS             838..1869
                     /gene="ispH"
                     /locus_tag="PLP95_07145"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0015968 - stringent response [Evidence
                     IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HPB45612.1"
                     /translation="MAVDHVLLAAPRGFCAGVEMAIKALAWMVRAFEPPVYCYHEIVH
                     NQRVVSRFTDLGVIFVDDISEVPEGSPIMLSAHGSAPEVVAHATERGGYVVDSVCPLV
                     TKVHHEVKVRADKGYQIVYVGHEGHEEAVGTMAVAPLSTHRVESVDDVAALPQFEEPV
                     ALLAQTTLSHRDWSDVLDATKERFPDMWVPGRSDLCFATTNRQTALMTIAPRCDAMVV
                     VGSANSSNTLALEKLAREAGCPRVFRVNGADELPDDLAGIVGVTAGASAPDELVDAVI
                     AKLAPVNGVEEVRTTDEDEYFPPPRNLRDLLVAVDAFATFAVGGNPIDHRVLNDRKLR
                     AGDVLSSLA"
     gene            1895..2263
                     /locus_tag="PLP95_07150"
     CDS             1895..2263
                     /locus_tag="PLP95_07150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB45613.1"
                     /translation="METLRLSLHILGACIWVGGQVVMGSLVPVLRKYGPDAPKAAARQ
                     FNMVGWAGFGLAFVTGMWNMMVDDELDQMLFGIKFLVVLISAAGALIHIVGTSKPALA
                     AGGAIATVAGIASIFLGVAL"
     gene            2287..>3656
                     /locus_tag="PLP95_07155"
     CDS             2287..>3656
                     /locus_tag="PLP95_07155"
                     /inference="COORDINATES: protein motif:HMM:NF015043.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carotenoid oxygenase family protein"
                     /protein_id="HPB45614.1"
                     /translation="MTPHPGPRRPRSDQPPTRRDLLRWGAHGLAAVPFGALIGCSGSD
                     RDSASRASSTTSGASASTSTTAALRTDPSGRWWLEGNYAPVTREVEAFDLNVVQGAIP
                     SDLNGLYVRNGSNPQSGESLMWFFGDGMAHGVSLGGGSAAWYRNRYVNTTLYQQNLGF
                     GDSVPGGAANQSNVSFFHHAGRLMSSGEVGAPYELDPADLSTIGVHTFDSALTSAFTA
                     HPKIDPDTGHLHSFGYGFVAPYLTYHVTDTTGAMIHSEPVEVPRSTMMHDFMITDRDV
                     IFMDLPVVFDLDAASRWMADPTVAATPFQWQPEVGARLGVMPLGGPASAITWYDIDPC
                     YVFHSVNAYRDGDTIVMDVCRQESMFAADMVGLGGGLTLRRWTLNTVTGSFDDTVVEQ
                     DNPGELPTRDPRRVGRRHRYGYLVETHELDDGTPAFGGLIKHDFDRDTRDVWTPSDGE
                     QASEWL"
BASE COUNT          594 a         1263 c         1178 g          621 t
ORIGIN      
        1 ggatgcgcaa aaggtcctgg ccctggttcc gggcacgggc cgggatttgt ggaccgacct
       61 gtcgcggttg atgacgacgc gctggtttac ggcctatgtc cgcatcctgg ccgcggccat
      121 ggccgaggcc ggccggcctg tgtggctgta ccgctttaac gcgccgccgc cgttcggtgc
      181 gcttggcgtg ctcagggacg agtcgggcgc gtccggggtc accgtcgccc aggaggtgaa
      241 acggccggtg ttgacgacga ccaacgcgtt gcccgaggcc gtaccggagg acaacttgtc
      301 gaggttcacg accagcgaaa cctccgaggt cgactcgtcg gaccaccgca aggtcgccgt
      361 gccggtgcca gcccccgtcg tgcagcccat ctcggggaat tcaatcgcca acccctccat
      421 cgaaaggctc gtgagctccg acccggtctc atcgtcacag tcggtcgacc ccgtccattc
      481 ggtggcggca gcctgcacgg cctggacctc ctcggtgacc gctggagtga actcgactcc
      541 gccgccgagc gaaccgcagc tcacgcgggt gaaatcgggt accgcaatcg gctcttgcac
      601 acaccctgag accgctgcgg cggcgagggc gcctgcacct atgacgagca acttcttgga
      661 tttcatgatc cccctcttgg cccgccccct cgaccgcacg ttctaacgat cgccgaacgg
      721 cggcaccgtc cggatttgtg acattagcgc caaaaccgac gccgcaacac agaccggcgc
      781 cggaaacatg tccgcgctgc aacggagagt tccgcgcacg ctcctacact tggggccatg
      841 gctgtcgatc acgtcctgct cgccgctccc cgaggcttct gcgccggggt cgagatggcg
      901 atcaaagcgt tggcgtggat ggtgcgggcc ttcgagccgc ccgtgtactg ctaccacgag
      961 atcgttcaca accagcgggt cgtgagccgc ttcaccgacc tcggcgtcat cttcgtcgac
     1021 gacatctccg aggttcccga gggctccccc atcatgttgt cggcccacgg ctcggccccc
     1081 gaggtcgtcg ctcatgccac cgagcggggc ggctacgtcg tggactcggt gtgcccgctg
     1141 gtcacgaaag ttcaccacga ggtgaaggta cgcgccgaca agggctatca gatcgtctat
     1201 gtcggccacg agggccacga ggaggccgtg ggaacgatgg cggtcgcccc gttgtcgacc
     1261 catcgggtcg agtcggtcga cgacgtcgcc gcgctgcccc agttcgagga gcccgtcgca
     1321 ctgctcgccc agacgacctt gagccaccgc gactggagcg acgtgctcga cgccaccaag
     1381 gagcggttcc ccgacatgtg ggtgccgggc cgcagcgacc tgtgtttcgc cacgacgaac
     1441 cggcagaccg cactcatgac gatcgcgcct cgttgcgatg ccatggtggt cgtggggtcg
     1501 gcgaactcgt ccaacaccct cgcgctggaa aagctcgccc gcgaagccgg atgcccacgg
     1561 gtgtttcggg tcaacggcgc cgacgagttg cccgacgatc tcgccggcat cgtcggggtc
     1621 acggccggcg cctccgcacc cgacgaactc gtggatgcgg tcattgcaaa gctcgccccg
     1681 gtcaacgggg tcgaagaggt ccgcacgacc gatgaggacg agtatttccc gccgccgagg
     1741 aacctgcgcg acctcctcgt cgcggtcgac gcgttcgcca cgttcgccgt cggcggcaac
     1801 ccgatcgacc accgggtcct caacgaccgc aaactccgcg ccggcgacgt gttgagttcg
     1861 ctcgcgtagc cggccgccgc tagaacacct cggcatggaa acgcttcgtc tctcgcttca
     1921 catcctcggc gcgtgcatct gggtcggcgg ccaggtcgtg atgggatcgc tggttcccgt
     1981 gctgcgcaag tacggtcccg atgctccgaa ggcggcagct cgacagttca acatggtcgg
     2041 ttgggccggg ttcgggctcg cgttcgtgac cgggatgtgg aacatgatgg tcgacgacga
     2101 actcgaccag atgctgttcg gcatcaagtt cctcgtcgtg ttgatttccg ccgccggcgc
     2161 gctgatccac atcgtcggga cctcaaagcc cgccctggct gctggcgggg cgatcgcgac
     2221 agtcgccggc atcgcgtcga tcttcctcgg cgtcgcgctg tgacgaaccg gtaacctccc
     2281 gtggcgatga cgccacaccc cggaccacgt cgaccacgtt ccgaccagcc gccgacgcgc
     2341 cgagacctgc tgcgctgggg cgcgcacggg cttgccgcgg tgccgttcgg ggcgctcatc
     2401 gggtgctcgg gctccgatcg ggactcggcg agcagggcat cctcgacgac ctcaggtgcg
     2461 tcggcctcga cctccacgac cgctgccctg cgcaccgacc cttcaggtcg ctggtggctc
     2521 gagggcaact acgcgccagt gaccagggag gtcgaggcgt tcgacctcaa cgtcgtgcag
     2581 ggcgcgatcc cgagcgacct caacgggctc tacgtccgca acgggtcgaa cccgcagtcg
     2641 ggcgaatcgc tcatgtggtt cttcggcgac ggaatggccc acggggtcag cctcggcggg
     2701 ggctccgcgg cctggtatcg gaaccgctac gtaaacacca cgctgtatca gcagaatctc
     2761 gggttcggag actcggtgcc cggcggagcg gcgaatcaat cgaacgtgtc gttctttcac
     2821 cacgccggac ggctgatgtc gagtggtgag gtgggggcgc cctacgaact cgaccctgcc
     2881 gacctgtcga ccatcggtgt gcacaccttc gactcggcgt tgacctcagc gttcacggcg
     2941 cacccgaaga tcgaccccga caccggccat ctgcacagct tcggctacgg attcgttgcg
     3001 ccgtatctca cctatcacgt caccgatacg accggagcga tgatccactc cgaaccggtc
     3061 gaggttcccc gctcgacgat gatgcacgac ttcatgatca ccgaccgcga cgtcatcttc
     3121 atggaccttc ccgtcgtgtt cgacctcgat gcggcatcgc ggtggatggc cgaccccacc
     3181 gtggccgcaa cgccgttcca gtggcagccc gaggtcggcg cacggctcgg agtgatgcca
     3241 ctcggcggtc cggcgagtgc catcacctgg tacgacatcg acccgtgtta cgtgttccat
     3301 tcggtgaatg cgtatcgcga cggcgacacg atcgtgatgg acgtctgtcg ccaggagtcg
     3361 atgttcgcgg ccgacatggt ggggctcggc ggcgggttga cgctgcggcg ctggaccctg
     3421 aacaccgtga ccggctcgtt cgacgacacg gtggtggaac aggacaaccc cggcgaactg
     3481 ccgacccgcg acccgcgccg agtcgggcgc agacaccgct acggctacct cgtggagacc
     3541 cacgaactcg acgacggcac cccggcgttc ggcgggctga tcaagcacga cttcgaccgc
     3601 gacacccgcg acgtctggac cccctccgat ggcgaacagg cctcggaatg gctgtt
//
LOCUS       DAOOMA010000230         3645 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_30803_length_3645_cov_3.789694, whole genome shotgun sequence.
ACCESSION   DAOOMA010000230 DAOOMA010000000
VERSION     DAOOMA010000230.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 3645)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3645)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3645
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30803_length_3645_cov_3.789694"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(78..887)
                     /locus_tag="PLP95_07160"
     CDS             complement(78..887)
                     /locus_tag="PLP95_07160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002547050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPB45615.1"
                     /translation="MSGAGHDPDATALTAADPRAVIIRGLTKRFGDRHAVNGISLDVP
                     RGAFFGIVGPNGAGKSTTIKMCTTLLRPDAGDVWVDGHHVWSDVTVAKHRLGVLPDDL
                     PLFERLTGGEFLSFLGVFRGLAPGVVADRSAALMSVLGLDDAARVLIADYSTGMRKKI
                     SLAAAVLHNPRVLFLDEPFESVDPVSARTIQDVLHTYCVGGGTVILSSHVMDTVERLC
                     DHVAIVNAGMIVAAGTVEQVRGGRRLEDVFIDAVGGRTAQGGDLGWLGTEV"
     gene            complement(884..2284)
                     /locus_tag="PLP95_07165"
     CDS             complement(884..2284)
                     /locus_tag="PLP95_07165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium chelatase"
                     /protein_id="HPB45616.1"
                     /translation="MTRPATLGELKASGWESVPVKEELRRNAIAKIRAAEPLVAHVMG
                     YEDTVVPQIETALLAGHDIVFLGERGQAKTRIIRGLVDLLDEYMPIVAGSEINDDPYN
                     PISRYARDLVAEHGDDTHIDWVHRSERYGEKLATPDTSIADLIGEVDPIKVAEGRYLS
                     DELTLHYGLVPRTNRGIFAINELPDLAERIQVGLLNVLEERDVQVRGYKIRLPLDLVM
                     VASANPEDYTNRGRMITPLKDRFGAQIRTHYPLDVETEMAIIDQEAAPLQAAGLTVDV
                     PEFMTEIIATFSQLARESNHISQRSGVSVRLTVSNHESVVAAAARRALYAGESVVAPR
                     VCDLDAIAASTAGKIEIESIDSARDELVIRHLVNAAVLTVFRERVGPQYLISIAEAFE
                     GREPVVAGEDVGAQEYVSLCTEIAPLGEAVRSVTGSERPEVVASAVELVLEGLHLSKR
                     LNKEAFGGRAQYRSRA"
     gene            2410..3180
                     /locus_tag="PLP95_07170"
     CDS             2410..3180
                     /locus_tag="PLP95_07170"
                     /inference="COORDINATES: protein motif:HMM:NF015679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HPB45617.1"
                     /translation="MTARVRLRNLAVAAPLALALTLGATAPGAGAAPLQETPATEVPT
                     TAAPAPAPAPAGPVTLFYLPLSGPDLAKGQHSAEVFLMQIHLVLRGYHIQDFPGAFGE
                     STRHALTAFQKYHLLPRSGVLDPLSRFILGASLERVTPSFPTLGRNIEVDINRQIMIV
                     STNGVTDWVFDVSTGKSSTPTPRGSYRIQREINGLRVARLGRLWRPKYFHGGYAFHGY
                     PSVPNYNASSGCVRMYNPDIDMLWASGLAPVGTPVRIF"
     gene            complement(3320..>3645)
                     /locus_tag="PLP95_07175"
     CDS             complement(3320..>3645)
                     /locus_tag="PLP95_07175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB45618.1"
                     /translation="ASNMAESRILATDLVAKRTGNAEFRRLADRTNPGRVWVAQRADR
                     MWSASGRSAPRDEGTDGIELHPNALAGVAVLALGRLWRGLALLSDRKQRPVVLADLQR
                     QLARR"
BASE COUNT          582 a         1220 c         1191 g          652 t
ORIGIN      
        1 gttctccgat aaccagtggt cgtgatcacg accactggtt atcggagaac gggcatttgg
       61 aggagggggc acggaagtca cacctctgtg ccgagccaac cgaggtcacc gccctgcgcg
      121 gtgcgcccgc cgaccgcgtc gatgaacacg tcttcgagcc gtcgcccgcc gcgaacctgt
      181 tcgaccgttc ctgcggccac gatcatgccg gcgttgacga tggcgacgtg gtcgcacagc
      241 cgctcgacgg tgtccatgac gtggctcgac aagatgaccg ttccaccgcc gacgcagtag
      301 gtgtgcaaca cgtcttgaat cgtccgcgcc gacaccgggt cgaccgattc gaacggctcg
      361 tcgaggaaca gcacccgcgg attgtgtaac acggccgccg ccaggctgat cttcttacgc
      421 atgccggtcg agtagtcggc gatcagcacc cgggccgcgt cgtcgaggcc gagcaccgac
      481 atgagcgcgg ccgagcgatc ggcgacgacg cccggcgcga gcccacgaaa taccccgaga
      541 aagctgagga actccccgcc ggtcagccgc tcgaacagcg ggaggtcgtc ggggagcaca
      601 cccaatcgat gcttcgccac cgtgacgtcg ctccagacgt gatggccgtc cacccacacg
      661 tcgccagcat cgggccgcag cagcgtcgtg cacattttga tcgtcgtcga cttgccggca
      721 ccgttgggac cgacgatgcc aaagaaggcg ccccgcggca cgtcgaggct gatgccgttg
      781 accgcgtgac ggtcaccgaa acgcttggtc aacccgcgga tgatgacggc ccgcgggtcg
      841 gcagcggtga gggcggttgc gtccgggtcg tgtccggcgc cgctcatgcc cgcgagcggt
      901 actgggctcg ccctccgaac gcttctttgt tgaggcgctt cgacaggtgc agcccttcga
      961 gcaccaactc gacggcgctg gcgacgacct cggggcgttc cgagccggtc accgagcgca
     1021 ccgcctcgcc gagcggcgcg atctcggtac acagcgagac gtactcctgc gctccaacgt
     1081 cctcgccggc gacgaccggt tcgcgccctt cgaacgcctc ggcgatgctg atgaggtact
     1141 gcgggccgac gcgctcgcga aacacggtga gcaccgcggc gttcacgagg tggcggatga
     1201 cgagttcgtc gcgggcgctg tcgatcgatt cgatctcgat cttgccggcg gtcgacgcag
     1261 cgatggcatc gaggtcgcag acccgaggcg caaccaccga ctcaccggca tacagcgccc
     1321 ggcgcgccgc ggccgccacc acggactcgt ggttcgatac ggtcaggcgc accgacaccc
     1381 ccgagcgctg gctgatgtgg ttcgactcgc gtgccaactg actgaacgtg gcgatgatct
     1441 cggtcatgaa ctcaggaacg tcgacggtga gcccggcggc ctgaagcggc gccgcttcct
     1501 ggtcgatgat cgccatttcg gtctcgacgt cgagcgggta gtgggtgcgg atctgggcgc
     1561 cgaaacgatc cttgagtggg gtgatcatcc gaccgcggtt cgtgtaatcc tccgggttgg
     1621 ccgaggccac catgacaagg tcgagtggga ggcggatctt gtagccacgc acctgcacgt
     1681 cgcgttcttc gagcacgttc aacaggccga cctggatgcg ctcggcgagg tcgggcagtt
     1741 cgttgatcgc gaagataccg cggttggtgc gcggtacgag gccgtaatgc agcgtgagtt
     1801 cgtcggacag gtagcggcct tcggcgactt tgatcgggtc gacctcgccg atgagatcgg
     1861 cgatcgaggt gtcgggggtg gcgagctttt ctccgtaccg ttcgcttcga tgcacccagt
     1921 cgatgtgagt gtcgtctccg tgttcggcca cgaggtcccg cgcatagcgc gagatcgggt
     1981 tgtacgggtc gtcgttgatc tcgctgccgg cgacgatcgg catgtactcg tcgagcagat
     2041 cgaccagacc gcggatgatg cgggtcttgg cctgaccgcg ttcgccgagg aacacgatgt
     2101 cgtggccggc gagcagggcc gtctcgatct ggggaacgac cgtgtcttcg taccccatga
     2161 catgagcgac gagcggctcc gcggcacgga tcttcgcaat ggcgttgcga cgcaactcct
     2221 ccttcaccgg gaccgattcc cagccggatg ctttgagttc gccgagcgtt gcgggtcgag
     2281 tcatgcgcgg cacgatatcg cgtgacggtg aggccccggc tgcgcgaccg gggttggtcg
     2341 gggtttggtc ggaacgattc gcgtggggtc cggcatgatt ggccctttgc gccgatagcc
     2401 tctcttgtca tgactgcacg tgtccgactt cgcaatctcg ccgtcgccgc gccactcgcg
     2461 ttggcgttga cccttggcgc cacagcccct ggcgccggtg ccgctcccct ccaggagacc
     2521 cccgcgaccg aggtcccgac gactgcggcg cccgcacctg cgccggctcc cgccggtccg
     2581 gtcacgctgt tctatctccc gttgtcgggc cccgacttgg ccaagggtca acacagcgcc
     2641 gaagtgttcc tgatgcagat ccacctggtg ctgcgcggct atcacatcca ggacttcccc
     2701 ggagcgttcg gcgagtcgac ccgccacgcg ttgaccgcgt tccagaagta ccacctcctg
     2761 ccgcggtcgg gcgtgctcga cccgctgtcg cggttcatcc tcggcgcttc gctcgagcgc
     2821 gtgacgccgt cgtttccgac gttgggtcgc aatatcgagg tcgacatcaa ccgtcagatc
     2881 atgatcgtgt cgaccaatgg cgtgaccgat tgggtcttcg acgtctcgac cggcaagtcc
     2941 tccactccga cgccgcgcgg ttcgtacagg atccaacgcg agatcaacgg gttgcgcgtc
     3001 gcacggctcg gtcgactgtg gcgccccaag tacttccacg gtgggtacgc gttccacggg
     3061 tacccgagtg tgcccaacta caacgcctcg agcggttgcg tgcgcatgta caaccccgac
     3121 atcgacatgt tgtgggcgag cggtctcgcc ccggtcggca cgccggttcg catcttctaa
     3181 accacccggc cactaaccaa cctgcctgcc cccagcctgt tctccgataa ccagtggtcg
     3241 tgatcacgac cactggttat cggagaacgg cgtttttggt ggcgttcttg gtggcgtttt
     3301 gggcagcgat cagcgtcgat cagcggcggg ccagttgccg ctgcaggtcg gcgagcacca
     3361 ccgggcgttg cttgcgatcc gacaacaacg ccagaccgcg ccacagccgg cccagcgcca
     3421 gcaccgcgac tccggccaga gcgttcggat gcaactcgat gccatcggtt ccctcgtcgc
     3481 gcggagccga acggcccgag gcgctccaca tgcgatcggc gcgttgggcc acccacaccc
     3541 tgccgggatt ggtcctgtcg gcgaggcgcc ggaactcggc attacccgtt cgcttcgcga
     3601 ccaggtccgt ggcgagaatc cgcgactccg ccatgttcga cgcgc
//
LOCUS       DAOOMA010000231         3645 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_30805_length_3645_cov_3.294150, whole genome shotgun sequence.
ACCESSION   DAOOMA010000231 DAOOMA010000000
VERSION     DAOOMA010000231.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 3645)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3645)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3645
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30805_length_3645_cov_3.294150"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1098)
                     /locus_tag="PLP95_07180"
     CDS             complement(<1..1098)
                     /locus_tag="PLP95_07180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB45619.1"
                     /translation="MVVVLAGIIASIVWWNPPGSAADADVALGAPGLRTVPSPRAEPL
                     SDEEPVPGEVAVDPGPGAVHPSVAAPLDDAGFAHVRTVDPSRPVSGTNFHSLSEAVAA
                     LTPQRGVPRDDSKLIRVWEHADGYREGEIAIEVSNLTIANAPANSPRFVGTQRLSLAK
                     LDATTWVGHGAEVDAVDAMCFVDRHPASFDQDVFTTVVGQTLWHPALDPHQLFVGDTA
                     YTRTLGNLREHHGPAGGTYTVESPGTVTVALPQEVDVAPVAEFTTKRFALKVGDYARN
                     VTLIGLSFARYSPCLADTRATLELNGPGVSLIDVSVTESAATGIAVSRGTGLLEGAQL
                     IGVDASNNGASAMTVYERGENPIRNGADEWDP"
     gene            1414..>3645
                     /locus_tag="PLP95_07185"
     CDS             1414..>3645
                     /locus_tag="PLP95_07185"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF004044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009330 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) complex [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HPB45620.1"
                     /translation="MPPQGDLFESNFAREIVEIPVQQEMSESFLAYSLSVITSRAIPD
                     VRDGLKPVQRRILYSMQSMGLRPDRPHRKCAGVVGDTMGKYHPHGDGSIYDALTRMGQ
                     DFSRNIRLVDPHGNFGTLDDPPAAYRYTECRLSPAAMEMLDEIDEDTVEFRPTFDGER
                     DEPIYMPARLPNLLVNGTTGIAVGMATNMAPHNLSEVYEAIKLVMTQRRPKPTVEDLM
                     AVLPGPDLPSGGIIIDDSIAEAYATGRGSFRMRATAEIVNLTKSRQGIVVTELPYMVG
                     PERIVGRIQDLVNAGKLNGVSAVKNLQDRRTGMNITIECKPGVNAKAVLTELYKLTPM
                     EESFGINNVVLVNGVPTTVGIWDLCNYYAEHRLDVVRRRTAHRLQKAQDRLHILEGLL
                     IALDAIDLVISIIRSSQDVPEARARLQQELSLSEIQANHILDMQLRRLTALEKLKLEE
                     EVAALRDQIAEYEKILASEQRQRTIVLKELEEMVERYGAPRRSRIIRPDQIDDVSIES
                     MIEAPEGLVDEPCRITLSASGIVGREPAGGPKQSTFGRHDVLRTRVETRTTGKVIGIT
                     NTGRVLVSPAAAIPEITGRSRGVTQSEAFTTQKGESIVGLVAGPAPSAATATGDDLAG
                     NDEPTPSSDPWMIVTANGVVKRIAAKEITSTASTRTIVNLKSGDTVVAMFPAPDDAEV
                     VLVSSNAQALRISASTVTVQGRGAGGVAGMKLSEGAAVIAAAVVGSDAIVLTHTDAMT
                     AKVT"
BASE COUNT          658 a         1311 c         1114 g          562 t
ORIGIN      
        1 cgggtcccac tcgtccgctc cgttgcggat cgggttctcc ccccgctcgt agaccgtcat
       61 ggccgatgca ccgttgttcg aagcgtcgac accgatgagc tgtgccccct cgagcagacc
      121 cgtgccgcgc gaaaccgcaa tgccggtcgc cgcactttcg gtgaccgata cgtcgatgag
      181 cgacacgccg gggccgttga gttcgagggt cgcccgggtg tcggcgaggc agggcgagta
      241 cctcgcgaag ctcagtccga tcagcgtgac gttgcgggcg tagtcgccga ccttgagagc
      301 gaaccgtttc gtcgtgaact ccgccaccgg cgcaacatca acctcttggg gtagtgcaac
      361 cgtgaccgtg cccggggatt cgacggtgta ggtgccaccg gccggcccat gatgctctcg
      421 cagatttccc agcgttcggg tgtaggccgt atcgccgacg aaaagctgat gtgggtcgag
      481 cgccggatgc cacagggtct ggccgaccac cgtcgtgaac acgtcctggt cgaacgatgc
      541 cgggtgccgg tcgacaaagc acatggcgtc gacggcatcc acctccgcgc cgtgccccac
      601 ccacgtcgtg gcgtcgagct tcgccagcga cagtcgctgt gtgccgacga accgcggtga
      661 attcgcggga gcgttcgcga tcgtcaggtt gctcacctcg atcgcgatct cgccctcccg
      721 atagccgtcg gcatgctccc acacgcggat gagtttcgag tcgtccctcg gcaccccccg
      781 ttgcggggtc agcgcggcaa ccgcctcgct caacgagtga aagttggtgc cgctcaccgg
      841 acggctcggg tcgaccgtcc tcacgtgggc gaatccggca tcgtcaagcg gggccgcgac
      901 gctgggatgc accgcccccg gccccggatc gacagcaacc tccccaggca cgggctcctc
      961 gtccgacagc ggctccgccc gaggactcgg aaccgtcctc aaacccgggg cacccagcgc
     1021 aacgtccgca tcggccgccg aacccggagg gttccaccac acgatgctcg cgatgatgcc
     1081 ggccagcacg accaccacgg cgcatccgat cactcggcgt tccggccggc gcggacgacg
     1141 accgatttca cctcgctgct ccggcggccc cgtagcttcg ccgtgcccat ggttcgccga
     1201 ccgatccccg cccaccggca caggctaccc tcgcttcggt tcgacgagca tgcggcgtag
     1261 atcgaccggc cgctgtccgc gagcggttcc gcgagagcct ctagactcgt cgacgaacgt
     1321 gcgttctgcc cgcgtcgcag gcggtgctgc cttcgccccc gcgaggcccc aacacgcacc
     1381 cgttgtggtg ccccccatca gctggagacc ttcatgcccc ctcaaggcga tctgttcgag
     1441 tcgaacttcg cacgcgaaat cgtcgagata cccgtccaac aggagatgag cgagagcttc
     1501 ctcgcctatt ccctgtcggt gatcacctcc cgcgccatcc ccgatgttcg cgacgggctc
     1561 aagccggtgc aacgccgcat cctgtactcg atgcagtcga tgggcctgcg ccccgaccgg
     1621 ccccaccgca agtgcgccgg tgtcgtcggc gacacgatgg gcaagtacca cccccatggg
     1681 gacgggtcga tctacgacgc actcacccgc atggggcagg acttcagccg caatatccgc
     1741 ctcgtcgacc cccacggcaa cttcggcacc ctcgacgatc cgcctgccgc gtaccgctac
     1801 accgagtgcc ggctatcccc agccgcgatg gagatgctcg acgaaatcga tgaggacacg
     1861 gtcgagttcc gccccacctt cgacggggag cgcgacgaac ccatctacat gccggcgcgg
     1921 ctgcccaacc tgttggtcaa cggcaccacg ggcatcgcgg tcggcatggc gacgaacatg
     1981 gcgccacaca acctcagcga ggtgtacgag gcgatcaagt tggtcatgac ccaacgtcga
     2041 cccaaaccga ccgtcgaaga tctcatggcg gttctgcccg gaccggacct cccctcgggg
     2101 ggcatcatca tcgacgactc gatcgccgag gcctatgcga ccggacgcgg atcgtttcgc
     2161 atgcgagcca ccgccgagat cgtgaacctc accaagtcgc gccaaggcat cgtcgtcacc
     2221 gaattgccct acatggtcgg ccccgagcgc atcgtcggac gcatccagga cctcgtcaac
     2281 gccggcaagc tcaacggcgt ctcggcggtc aagaacctcc aggaccgccg tacggggatg
     2341 aacatcacca tcgaatgcaa gccgggggtc aacgccaagg ccgtgctgac cgagctctat
     2401 aagctcaccc cgatggagga gagcttcggc atcaacaacg tcgtgttggt caacggcgtc
     2461 cccaccacgg tcgggatctg ggacctgtgc aactactacg ccgagcatcg cctcgacgtc
     2521 gttcgccgac gcaccgccca ccggctgcag aaggcacagg accggctgca catcctcgag
     2581 ggtctcctca tcgcgctcga cgccatcgac ctggtcattt cgatcatccg ctcgagccag
     2641 gacgtacccg aggcccgggc gcggctccaa caggagcttt ccctcagcga gatccaggcc
     2701 aaccacatcc tcgacatgca gcttcggcgc ctcacggccc tcgagaagct caagctcgaa
     2761 gaagaggttg cggcgttgcg cgaccagatc gccgagtacg aaaagatcct cgcctcagag
     2821 cagcgccagc gaaccatcgt gttgaaggaa ctcgaggaga tggtcgagcg ctacggggcc
     2881 ccgcgccgca gccgcatcat ccgccccgac cagatcgacg acgtctcgat cgagtcgatg
     2941 atcgaagccc ccgagggtct cgtcgatgag ccgtgccgaa tcaccctgtc ggcctccggc
     3001 atcgtcggcc gcgaaccggc tgggggtccg aagcagtcga cgttcggccg ccacgacgtc
     3061 ctgcgaacac gggtcgaaac ccgaacgacc ggcaaggtga tcggcatcac gaacacgggt
     3121 cgggtcctgg tgtcacccgc cgcggcgatc cccgaaatca ccggccggtc gcgcggggtg
     3181 actcaatccg aagccttcac cactcaaaag ggcgagtcca tcgtcgggct ggtggccggg
     3241 cccgcaccga gcgcggcaac ggccaccggc gacgaccttg cgggcaacga cgagccgacg
     3301 ccgagttccg acccctggat gatcgtgacc gccaatggcg tcgtgaagcg catcgcggcc
     3361 aaggagatca cctccaccgc cagcacgcgc acgatcgtga acctcaagag cggcgacacc
     3421 gtcgtcgcca tgtttcccgc accggatgac gccgaggtcg tgctggtcag ctcgaatgcc
     3481 caagcccttc ggatttcggc atcgacagtg acggtacaag gccgtggtgc cggcggcgtc
     3541 gcagggatga agctctccga gggtgccgcc gtcatcgccg ccgcggtcgt cgggtcggac
     3601 gcgatcgtgt tgacgcacac cgacgccatg accgccaagg tgacc
//
LOCUS       DAOOMA010000232         3642 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_30838_length_3642_cov_4.133538, whole genome shotgun sequence.
ACCESSION   DAOOMA010000232 DAOOMA010000000
VERSION     DAOOMA010000232.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 3642)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3642)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3642
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30838_length_3642_cov_4.133538"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1155
                     /locus_tag="PLP95_07190"
     CDS             <1..1155
                     /locus_tag="PLP95_07190"
                     /inference="COORDINATES: protein motif:HMM:NF019368.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004559 - alpha-mannosidase activity
                     [Evidence IEA];
                     GO_process: GO:0006013 - mannose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 38 C-terminal
                     domain-containing protein"
                     /protein_id="HPB45621.1"
                     /translation="VEGSSIELDNAVVAVRIDAEGHVSSMVHHETGREVVAPGSVANV
                     LQTFEDLPTHFDAWEIEDYTLRRPREQREVDSIEVLETGPLVARVEVRRSFGSSKVVQ
                     RYVLRAGSPRLDIECEVDWRERETLLKVAFPIDVHSTSLTREVQLGECSTPIHTNTSW
                     DAARFEVCALRWVDCSEPGFGVALLNDSKHGHDATRIVADGGDRPAVQLRLSLLRSSR
                     YPDTEQDQGAHRFTYSLMPHDGDRHGAGVAAQAWLLNNTIREFGGEGGDPGQAEADPF
                     VANGVTIASSSSCAVVIEAVKAADDGSGDIVVRCHESTGARGPVELRVGFAASAVQMV
                     DGRERCASVSRHVRSVESSLRWESNQIATSVTFSIRPFEIVTLRLRPRPT"
     gene            complement(1166..2485)
                     /locus_tag="PLP95_07195"
     CDS             complement(1166..2485)
                     /locus_tag="PLP95_07195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB45622.1"
                     /translation="MPTSVELTGLSSSLIEDIMLRIIIAEGRPTMVGVAQRMHCATGM
                     IESIATSLRDRRLIEYEGMQGRTYVLSVTEQGRSQSRVRSEECKYVGPIPVPLDLYSR
                     VVRAQTPELSLNHEQLTRAFSDLVLSPSLIDQLGPAICGDGAIFLYGPPGTGKSSIAE
                     RLIRAYGDSVLVPYCVEVQGQIISVFDPVLHEPLEQQPDELDPRWVLCQRPAIIAGGE
                     MHAGMLDLSLDRETGIYLAPLQFKANNGILVIDDFGRQNMTPEELLNRWIVPLDRQVD
                     YLTLGGRKFQVPFEVKVILSTNLDPESLGDEAFFRRIHNKVYIGCLDNEQFGYVLDRV
                     ARAKRLQFTEPAARRLRDVTRSRGDGELRAYLPGVVCKLAEAIIRYENLSPVITPELV
                     DRVLELYFAKQVKVGEPTGSQVVMPAPVAEPPVAPTRPSALASPFGD"
     gene            2731..3111
                     /gene="arsC"
                     /locus_tag="PLP95_07200"
     CDS             2731..3111
                     /gene="arsC"
                     /locus_tag="PLP95_07200"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00014.1"
                     /note="This arsenate reductase requires both glutathione
                     and glutaredoxin to convert arsenate to arsenite, after
                     which the efflux transporter formed by ArsA and ArsB can
                     extrude the arsenite from the cell, providing resistance;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0008794 - arsenate reductase
                     (glutaredoxin) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (glutaredoxin)"
                     /protein_id="HPB45623.1"
                     /translation="MADVTVYHNPNCNSSKTALKIAADAGIEVDVVQYLKTKPDAAEL
                     QGIVDKLQNPVTELVRRDANFKDLGLAEEDVQTAQQVVPLLAAHPKLLQRPIVVVGDR
                     AVVGRPKERIFELLGLDGDSSDES"
     gene            3180..>3642
                     /locus_tag="PLP95_07205"
     CDS             3180..>3642
                     /locus_tag="PLP95_07205"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF012310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HPB45624.1"
                     /translation="MAFELPALPYAIDALAPHISQETLEFHHGKHHNAYVTTLNNLIA
                     GTAQENASLEEIILAADAGPLFNNAAQVWNHTFYWNSMSPNGGGAPTGDLAAKIDAAF
                     GSLDGFKEQFTQAGMTQFGSGWAWLIVNGSGDLEITKTPNADLPLKHGQKAL"
BASE COUNT          673 a         1126 c         1175 g          668 t
ORIGIN      
        1 gtcgagggca gctcgatcga gttggacaac gccgtcgtgg cggtgcggat cgatgccgag
       61 ggccacgtgt cgtcgatggt gcatcacgag acgggccgtg aggtcgtggc tcctggctcg
      121 gtcgccaacg tgctgcaaac cttcgaggat ctacccaccc acttcgatgc ctgggagatc
      181 gaggactaca cgctgcgacg gccgcgcgag caacgtgagg tcgacagcat cgaggtgctc
      241 gaaacgggac cgctcgtcgc ccgggtcgag gtgcgacggt cgtttggctc ctcgaaggtc
      301 gtgcagcggt atgtccttcg ggcgggttca ccccgtctcg atatcgaatg tgaggtcgat
      361 tggcgcgaac gcgaaacgtt gttgaaggtg gcgttcccga tcgatgtgca ctcgacctcg
      421 ctcacccgcg aggtgcaact cggggagtgc tcgaccccga tccacaccaa caccagctgg
      481 gacgccgcac gtttcgaggt gtgcgcgttg cgctgggtgg attgcagcga gccgggtttc
      541 ggtgtggcgc tgctgaacga ctccaaacac ggccacgatg cgacccggat cgtggccgac
      601 ggtggagatc gtccagcggt gcaactgcgg ctgtcgttgc tgcgaagttc caggtatccc
      661 gacaccgaac aggaccaggg agcacatcga ttcacctact cgttgatgcc acacgacggt
      721 gatcgccatg gcgccggcgt ggcggcccag gcatggttgc tcaacaacac catccgtgag
      781 ttcggaggcg agggaggcga ccccggccag gcagaggctg atccgttcgt cgccaacggc
      841 gtcaccattg cgtcctcgtc gtcttgcgcg gtcgtgatcg aggcggtcaa ggccgccgac
      901 gacggcagcg gcgacatcgt ggttcgttgc catgaatcca ctggcgcgag aggcccggtg
      961 gagctgcgcg tcgggttcgc tgccagcgcg gtgcagatgg tcgacgggcg ggagcgctgc
     1021 gcttcggtgt cgcgccatgt ccgcagcgtc gaatcctcgc tgcgatggga atcgaaccag
     1081 atcgccacct cggtgacctt ttcgatccgc ccattcgaga tcgtcaccct gcgcctgagg
     1141 cctcgtccga catgagcccg acgagtcagt cgccgaacgg tgacgccaac gctgaaggac
     1201 gggtcggagc gacaggtggc tcggccactg gtgcaggcat caccacctgc gatccggtcg
     1261 gctcgccgac cttgacctgt ttggcaaagt acagctcaag cacccgatcg acgagttcgg
     1321 gcgtgataac cgggctgaga ttttcatagc ggatgatcgc ctcagccagc ttgcacacga
     1381 ccccgggcag gtacgcgcgc agctcgccgt cgccccgtga gcgtgtgaca tcgcgcagcc
     1441 ggcgcgctgc cggttcggtg aactgcaacc gctttgctcg agcaacgcgg tcgagtacgt
     1501 agccgaactg ttcgttgtcg agacagccga tgtacacctt gttgtggatc cggcgaaaga
     1561 acgcctcgtc tcccaagctc tccgggtcga ggttcgtcga caagatgacc ttgacctcga
     1621 atggaacctg gaacttccgg ccgccgaggg tcagatagtc gacctggcgg tccaggggga
     1681 cgatccaacg gttgagcagc tcttccggag tcatgttctg acgtccgaag tcgtcgatga
     1741 ccaggatccc gttgttggcc ttgaactgca gcggcgccag gtagatgccg gtctcgcgat
     1801 cgagactgag gtccaacatg ccggcgtgca tctcgccgcc agcgatgatc gctggccgtt
     1861 ggcacagcac ccaacgaggg tcgagctcat cgggctgctg ttcgagcggc tcgtggagca
     1921 cagggtcgaa caccgagatg atctgaccct ggacttcgac gcagtagggg accagaacag
     1981 agtcgccata ggctcgaatc aaacgttcgg cgatcgacga cttgccggtg cctggcggcc
     2041 catacaggaa gatcgcgccg tcgccgcaga tcgcaggacc gagttggtcg atcagactcg
     2101 gagaaagcac gaggtcggaa aacgcccggg tgagctgctc gtggttcaac gagagttccg
     2161 gggtctgggc acgaacgacc cgagagtaga gatcgagggg aaccggaatg ggtccgacgt
     2221 atttgcattc ctcgctgcgg acccgggact gggagcggcc ttgctcggtg accgacagca
     2281 cgtaggtccg accctgcatg ccctcgtatt cgatcaggcg acggtctcgc agcgaggttg
     2341 cgatcgactc gatcatgccg gtggcgcagt gcatgcgctg ggccactccg accatcgtcg
     2401 gacggccttc agcgatgatg atccgcaaca tgatgtcttc gatgagcgat gacgacagcc
     2461 cggtgagttc gacgctcgtg ggcatccgta gatcgtttgc gctcagcgct gggtcaaccg
     2521 ccatgtgtcc tcggtcctct agccggccag cgtcggccgc gaagttgtgc ctgttcgtca
     2581 tcggcaggtg ccgccggtgc ttccttgtct tcatcggcat ggtcccgacg agcttgagga
     2641 attcggccca tgcagcgtcc gctcctgcgc gaatcgctcg tggcttcgtt gcggctggcg
     2701 aggtcgagct gtcactgcaa cgctggttcc atggcagacg tcaccgtgta tcacaacccc
     2761 aactgcaact cgtccaagac ggcgttgaag atcgccgcgg acgcaggcat cgaggtcgat
     2821 gtggtccagt acctcaagac caagccggat gcagccgaac tgcaaggcat cgtcgacaag
     2881 ctgcagaacc cggtcaccga actggttcga cgcgatgcga acttcaagga cctcggcctc
     2941 gccgaggagg acgttcagac cgcgcagcag gtggtaccgc tgctggctgc tcaccccaag
     3001 ttgttgcagc gcccgatcgt cgtcgtcggc gaccgagcgg tcgttggccg cccgaaggag
     3061 cggatctttg aactcctggg gctcgatggc gactcgtccg acgagtcctg accgacgtgg
     3121 accatcgggt agtttgcaca tgttgagctc ccgaacaagc tctcgacaaa ggaagaatca
     3181 tggcttttga actcccggct ctgccgtacg cgatcgatgc gctcgctcct cacatttctc
     3241 aggagacgct cgagttccat cacggcaagc accacaacgc gtacgtcacg accttgaaca
     3301 acctcatcgc agggaccgcg caggagaacg cgtcgctcga ggaaatcatc ctcgccgccg
     3361 acgccggtcc gctcttcaac aatgccgctc aggtgtggaa tcacaccttc tactggaact
     3421 ccatgtcgcc gaacggcggc ggtgctccca ccggcgacct cgctgcgaaa atcgacgcgg
     3481 cgttcggttc gctcgacgga ttcaaagagc agttcaccca ggcgggcatg acccagttcg
     3541 gttcggggtg ggcgtggctt atcgtgaacg gctcgggcga cctcgagatc acgaagaccc
     3601 cgaacgctga ccttccgctc aagcacggcc aaaaggctct gt
//
LOCUS       DAOOMA010000233         3630 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_30989_length_3630_cov_3.916364, whole genome shotgun sequence.
ACCESSION   DAOOMA010000233 DAOOMA010000000
VERSION     DAOOMA010000233.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 3630)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3630)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3630
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30989_length_3630_cov_3.916364"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(308..613)
                     /locus_tag="PLP95_07210"
     CDS             complement(308..613)
                     /locus_tag="PLP95_07210"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HPB45625.1"
                     /translation="MVEDDDSTLVAVASWQDIVRIDLDGIWVEELAVATTHQHSGTGT
                     TAYELATDHLRTIDRDGDHLAALVHVSNTRSKRLLSTVGWQKTTDGDADHELWFGRL"
     gene            complement(781..1071)
                     /locus_tag="PLP95_07215"
     CDS             complement(781..1071)
                     /locus_tag="PLP95_07215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB45626.1"
                     /translation="MDEFTGSVLDVSGRTALLADEQGNALPIPASTSHEGLTPGAFVA
                     VDTERLDGGAATIWVRPAFVSDDDPHALHPGGVRLLTDAERHASREPAASTR"
     gene            complement(1517..2395)
                     /locus_tag="PLP95_07220"
     CDS             complement(1517..2395)
                     /locus_tag="PLP95_07220"
                     /inference="COORDINATES: protein motif:HMM:NF014713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcdA"
                     /protein_id="HPB45627.1"
                     /translation="MSTILAVTEQVGDGPLLIAMAIAFAAGVLSFLSPCVLPLVPVYL
                     SFTTGLSAADLAGATTEAAAPTPAIAGAEGTAATDGEATDPEPPTAPSRAAVRRRVVI
                     GSLGFVLGTAVVFVSFGALFGSFGEALRTHALGLSRIFGVVTIAFGLLMAGAFDKIGA
                     LQRDVRIHKVPGGGLLAAPVLGFTFALGWTPCIGPTLGTVLGLSASTSGATAARGAVL
                     SVAYCIGLGLPLLISGLAFTRAMSAFAVIRRHYQALMVAGGVCLIVLGLLQVTGVWTT
                     WTNSLQTRFGGWSLPL"
     gene            complement(2404..2955)
                     /locus_tag="PLP95_07225"
     CDS             complement(2404..2955)
                     /locus_tag="PLP95_07225"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HPB45628.1"
                     /translation="MTRPAPADRHWYRGRLRAATPLLGIRITMTVLALAAIATGCGGS
                     ESSAYTAADLRSGDAVSVDDLAGSPALLVSWATWCRECDEELAGLQQFAESLEAQGVR
                     IVAVNLDAADVEAEIDEKISRHDLSVELWRDRRNEFRGAFGALGVPTTVVIDANGKVV
                     GTFPGAVDFDDEAVLEALDTARS"
     gene            complement(2952..3269)
                     /locus_tag="PLP95_07230"
     CDS             complement(2952..3269)
                     /locus_tag="PLP95_07230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB45629.1"
                     /translation="MTIACIVTAGACSGTREPQTISIVIPAGTQDRLDAGEKVEIMPS
                     RLEFHVGDTLHIRNDDDVTQSIGPYLVKAGQDFRLLYGSPGVFEGYCPLSEGERYEIV
                     VKK"
     gene            complement(3338..>3630)
                     /locus_tag="PLP95_07235"
     CDS             complement(3338..>3630)
                     /locus_tag="PLP95_07235"
                     /inference="COORDINATES: protein motif:HMM:NF016221.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016531 - copper chaperone activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="copper chaperone PCu(A)C"
                     /protein_id="HPB45630.1"
                     /translation="ATAELHETRAAGGDMGGGDMGGDMGSSTTAPMMEMAEIDRLDIA
                     AGEAVSLQPGGYHIMLMNLAAPLTEGQELVVTLTFENAGDIEVTAVVGDGAP"
BASE COUNT          587 a         1143 c         1306 g          594 t
ORIGIN      
        1 tgccgcttgt ccgcgatcgc gtcggccacg gcctcggcca tgcgcacctg cgcgggccgg
       61 tcttcgtggg cggggaggcg accggtgacg ctgcgcagcg ttgcggcgac gtcgttgtag
      121 ctacccggat tcacccccac gagtgtggca ggcgaccgag gcagctgtgc aagccggtcg
      181 agcgtgggga gcgcaccctg tgtggggcga gtgaagcgat gcgccagatg agccgggagc
      241 gaccgccgcc atctgactgg cgatcgtctc gctcccgctc atcgagcgaa ttcgggggtc
      301 gcagccgtca gagtcggccg aaccagagtt cgtggtcggc gtccccgtcg gttgtcttct
      361 gccaaccgac ggtcgagagg aggcgtttgc tgcgcgtgtt gctgacgtgg acgagtgcgg
      421 cgaggtggtc accgtcgcga tcgatggtgc ggaggtggtc cgtggccagt tcgtaggcag
      481 tggttccggt accgctgtgc tgatgggtgg tcgcgactgc gagttcttcg acccagattc
      541 cgtcgaggtc gatgcgcacg atgtcctgcc aggacgcgac agccacgagc gtggagtcgt
      601 cgtcttcgac cagtgcgatc gcgcggcgtt ggaacgccgc tcatggcgca tcgttcaacc
      661 atgagcgtgc gttgtggcgg atccagtgct ctaccttgtc ctcgtaggga gcgccggtcg
      721 agcaggcgaa cgtggtgagc agctatgcgt cagctgcctc gttgtcgaac ggccgcagga
      781 tcaacgtgtc gacgcagccg gttcgcggga ggcgtgccgt tcggcgtcgg tcagcagccg
      841 aacgccgccg ggatgcagag cgtggggatc gtcatcggac acgaacgcgg gtcggaccca
      901 tatcgtggct gcgccaccgt cgaggcgttc ggtgtcgacg gcgacgaatg cgcccggtgt
      961 gagtccctca tggctcgtgc tcgctgggat ggggagtgca ttgccctgtt cgtctgcgag
     1021 gagcgcggta cggccggaca cgtcaagcac gctgccggtg aactcatcca cgccgagacg
     1081 gtcggagaat tgtgaccggg ctgatgcggt gagcaggccg agccgggtga gtgcgccgcc
     1141 cgtgtccgcg tcaacatcgg ccgggttcgg gtcgatgtcc ggattgtcga cgagccagtg
     1201 ggcgacgaac tgcgcggagc ggtgagcggc gagcgcttcg gcggcgtgga cgtccgcagc
     1261 gagatcggtg tttccgacgc ggaggagccg ggcgacatcg agtggtcatg cccgaccatg
     1321 cagcacggct gccgcgggga ccgagcggcg gcgatcacgc gtgctgagga gcgctgcgat
     1381 cccccgtgtt cgagagcgtc gagaaggtcg cgccatccgc gtcggtcacg gcgtcactag
     1441 ttcggctgtt tggaagacaa gccgatcgtc atctcaggcg agtaccgcgt tgccggcacc
     1501 cgcctcgctg gtgtcctcac aacggcaggc tccacccgcc gaatcgggtt tggagactgt
     1561 tcgtccaggt ggtccacacc ccggtgacct gcaacaggcc gagcacgatc aggcagaccc
     1621 cgccggcgac catcagcgcc tgatagtggc ggcgaatcac ggcgaatgcc gacatcgcac
     1681 gggtgaacgc gaggccgctg atcagcagcg gcaggccgag gccgatgcaa taggccaccg
     1741 acaacaccgc gccccgggcg gcggtggctc cggaggtgga tgccgacagg ccgagcacgg
     1801 tgcccagggt cgggccgatg cagggggtcc aaccgagtgc gaacgtaaac ccgagaaccg
     1861 gtgcggccag caggccgccg ccggggacct tgtggatgcg tacgtcgcgc tgcagcgctc
     1921 cgatcttgtc gaatgccccg gccatgagga ggccgaacgc gatggtgacc acgccgaaga
     1981 tccgcgacag gccgagcgcg tgggtgcgaa gcgcctcgcc gaagctgccg aagagcgctc
     2041 cgaaggaaac gaacaccacg gcggtgccca gaacgaaccc gagcgagccg atgaccacgc
     2101 gacgtcgcac cgccgcacgt gacggtgcgg tcggcggttc gggatctgtt gcttcgccat
     2161 cggtggcggc cgtgccctca gcgccggcga tggccggagt cggcgccgca gcctccgtgg
     2221 tcgccccggc gaggtcggcc gcggacaacc cggtggtgaa ggacagatag acgggcacga
     2281 gcggcaagac gcacggtgac agaaacgaca gcaccccagc agcgaaggcg atcgccatcg
     2341 cgatgaggag tggtccgtcg ccgacctgtt cggtgaccgc aaggatcgtc gacatggtgg
     2401 gacctagctc ctcgccgtgt cgagcgcttc gagcacggcc tcgtcgtcga agtcgaccgc
     2461 acccgggaag gttccgacga ccttgccgtt cgcgtcgatc acgacagtgg ttggaacacc
     2521 gagcgctccg aaggcgcctc ggaactcatt gcggcgatcc cgccacagtt cgaccgacag
     2581 gtcgtggcgc gagatcttct cgtcgatttc ggcctccacg tcggcggcgt cgaggttgac
     2641 cgcgacgatg cgcacccctt gtgcctccaa ggattcagcg aactgttgga gcccggcgag
     2701 ttcctcgtcg cattcacgac accacgtggc ccaactcacg agcagcgccg gggagccggc
     2761 gaggtcgtcg accgagaccg cgtcgccgct gcgcagatcg gcggcggtgt acgcgctgga
     2821 ttccgagcct ccgcagccag tcgcgatggc tgccaaagca aggaccgtca tcgtgatgcg
     2881 gattccgagg agcggggtcg cggctcggag gcgtcctcga taccagtggc ggtcggcagg
     2941 tgcgggacgc gtcatttctt caccacgatc tcgtatcgct cgccttcgct gagggggcag
     3001 tacccttcga acaccccggg agagccgtag agcaaccgga agtcctgacc cgctttgacg
     3061 agatacggtc cgatcgactg ggtcacatcg tcgtcgttgc ggatgtgcaa cgtgtcgccg
     3121 acgtggaact caagtcggga gggcatgatc tcgactttct caccggcgtc gaggcggtcc
     3181 tgggtgccgg caggaatgac gatcgagatc gtctgcggtt ccctcgtacc tgagcaggcg
     3241 cccgccgtga cgatgcatgc gatcgtcacg gcgagtaccg cccaggggat cccgagccga
     3301 tttcggagtg tccgatgccg aggtttcatg gctcagctca cggggcgccg tcaccgacga
     3361 cggcggtgac ctcgatgtca ccggcgtttt cgaaggtgag ggtcacgacc agctcctggc
     3421 cctcggtgag cggtgctgcg aggttcatca acatgatgtg gtatccgccg ggctgaaggc
     3481 tgacggcctc gccagcggcg atgtcgagcc ggtcgatctc ggccatctcc atcatgggtg
     3541 cggtggtcga ggagcccatg tcgccgccca tgtcgccgcc gcccatgtcg ccgccggcgg
     3601 cccgggtctc gtggagttcg gcggtcgccg
//
LOCUS       DAOOMA010000234         3623 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_31075_length_3623_cov_3.694226, whole genome shotgun sequence.
ACCESSION   DAOOMA010000234 DAOOMA010000000
VERSION     DAOOMA010000234.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 3623)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3623)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3623
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31075_length_3623_cov_3.694226"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(92..508)
                     /locus_tag="PLP95_07240"
     CDS             complement(92..508)
                     /locus_tag="PLP95_07240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB45631.1"
                     /translation="MPEAALVAVLVSVVVVAAATRRRQRGLRNSAAATTDLTVDDRSV
                     TRTLGDGRVESARWSALESVELVRTPVKTADGASSFLILAETAQLGCLVPLGVGLERE
                     VLERLATLRGFRVEKFNSALEKRPPHREFLWQRPEP"
     gene            723..2390
                     /locus_tag="PLP95_07245"
     CDS             723..2390
                     /locus_tag="PLP95_07245"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HPB45632.1"
                     /translation="MQNRSRRSVRIAALLLASSLVMSSCGGSDSGSDDDGDNNETTIA
                     NGTQDEGTPTPGGSLTYAIEADASGGYCLATAQLAAGGIQVANAIYDSLFALDENYEV
                     QPYLAESYSWNPEYTALSIKLREGITFHDGSPLTADVVKLNIDSWRGDAAAKEKTNLN
                     SLLFLFVFMNIAEVTVDDPLTVTIHTKTPWMALPQFLAGGRNGIMAESQMVGGEEHCK
                     ENLIGTGPFKVDNWTPNSEMKLSKNPNYWRTDAEGRQLPYLDSLTFKPIVGGTDRVDA
                     LEAGSIDAGHFSGQDQFDSITDNDALQLLKEAPGHMEIGYGLVNNAKAPTDDIEIREH
                     LGMAIDREELNEINSGGKFRTADQPFDTDVLGYLEDIETLEYDPDAAEEFFAGKNLSV
                     SIKYATDPTVKLLAESVAAQLKDVGVDSVVEVVDQAALIDQALGGNFNIILWRNHPGL
                     DPDTEYNWWHSDSPVNFGKINDPAIDDALDRGRVSTDPAERKTIYEDLNRAFAKGRYN
                     QWNWYTEWAVGASTKVHNITGSTLPDGSATPGVTWGWHSLAETWVDQ"
     gene            2493..3431
                     /locus_tag="PLP95_07250"
     CDS             2493..3431
                     /locus_tag="PLP95_07250"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPB45633.1"
                     /translation="MRLLGRRFLQLIVVIFLLSLVAFFSLRLIPGDPAEIIGLGADPA
                     TIEQIRDDIGLNESVVTQYTRWAGNFVRGDLGKYYHAAGDPAADVRRTLPISLQLMVY
                     TQIVALAFAIPLGVFAAYRADRLFDRMSNIGAFFAISIPAFVLAYLAKQYFVFKWNLL
                     PERGWPPLSEGVGDHFRHAVLPVAVMSVAQIAVYYRLLRSDMISTLQEDYITMARAKG
                     LGSQRILFRHALRPSSLTLLTVAGLNVGSMISTAVIVEKIFGIDGMGLKLVTAVFSRE
                     YIAAQSFIAVIGILFVLINFLVDLLYTVLDPRIRHA"
     gene            3436..>3623
                     /locus_tag="PLP95_07255"
     CDS             3436..>3623
                     /locus_tag="PLP95_07255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB45634.1"
                     /translation="MTDPAAETAVAVKASKQKLGAVGWVCAAWLILLVLVSLFGGLLK
                     ATPIEEGSRALRACGDGSG"
BASE COUNT          674 a         1230 c         1093 g          626 t
ORIGIN      
        1 tccgccgatt ccgccgctcg ccgaggtcgt cgaacccacc gccgtttcgc cgttcgtcgt
       61 ccccgccaac tgaggtgggg cgaacctgcg gttagggctc cggtcgctgc cagagaaact
      121 cgcggtgagg cgggcgcttt tcgagggccg aattgaactt ctcgacccga aacccgcgaa
      181 gcgtcgcaag ccgctcgagg acctctcgtt cgaggccgac cccgagcggc accaggcagc
      241 cgagttgcgc cgtctcggcg aggatcagaa acgacgaggc gccatctgcg gtcttgaccg
      301 gggtgcgcac caactcgacc gactccagcg ccgaccaacg cgccgactcg acgcgaccgt
      361 cgccgagggt ccgggtcacc gaccggtcat ccaccgtcag gtcggtcgtc gcggcggcgc
      421 tattgcgcag gccgcgctgg cggcgtcggg tggcggccgc caccacgacg acgctgacga
      481 gaacggcgac gagggctgct tcgggcacgg tcgacatcgt aggcccgaag gttctccgta
      541 tcgttgcgcc gacccgccgc ggcgatccgc gctgctcccc accaccaacc gagttgctac
      601 agtgctcccc tacttgatgt gccgaccgtc acaaacgcga cgggaagcac acggtcgcgg
      661 agggtgacct cccgaaccga cctcggcgcc gccccggcgg cacgaccatc tggagataca
      721 acgtgcagaa ccgatcacgg cgaagcgtcc gaatcgccgc tctcctcctc gcctcctcgc
      781 tcgtcatgag ctcgtgcggc ggcagcgaca gcggaagcga cgacgacggc gacaacaacg
      841 agacgacgat cgccaacggc acccaggatg agggcacccc gaccccgggc gggtcgttga
      901 cctacgccat cgaggctgac gcctcgggtg gttactgcct cgccacggcg cagctcgccg
      961 ccggaggcat ccaggttgcg aacgcgatct acgactcgtt gttcgcgctc gacgagaact
     1021 acgaggttca gccctacctc gcggagtcct attcctggaa ccccgagtac accgcgctca
     1081 gcatcaagtt gcgcgaaggc atcaccttcc acgacggatc gccactcacc gcggacgtcg
     1141 tgaaactcaa catcgattcg tggcgtggcg atgcggcggc caaagaaaag accaacctca
     1201 actcactgtt gttcctgttc gtgttcatga acatcgcaga ggtcacggtc gatgatccgc
     1261 tgacggtgac gatccacacg aagaccccgt ggatggcgct gcctcagttc ctcgccggcg
     1321 gccgcaacgg catcatggcc gagtcgcaga tggtcggtgg cgaggagcac tgcaaggaga
     1381 acctcatcgg aaccggaccg ttcaaggtcg acaattggac accgaactcg gaaatgaagc
     1441 tgtccaagaa tccgaactac tggcgtaccg acgccgaagg ccgccagctt ccctacctcg
     1501 actcgctgac gttcaagccg atcgtcggtg gaaccgatcg tgtcgatgcg ctcgaagccg
     1561 ggtcgatcga cgccgggcac ttcagtggcc aggaccagtt cgactcgatc accgacaacg
     1621 atgctctcca gctgttgaag gaagctccgg ggcacatgga gatcggctac ggcttggtca
     1681 acaacgccaa ggcccccacc gatgacatcg agatccgtga acatctgggc atggcgatcg
     1741 accgcgagga actcaacgag atcaactcgg gtggcaagtt ccgcaccgcc gatcagccat
     1801 tcgacaccga tgtcctcggc tacctcgagg acatcgagac gctcgagtac gacccggatg
     1861 ccgccgagga gttcttcgcc ggcaagaacc tcagcgtgag catcaagtat gcgaccgacc
     1921 cgacggtgaa gctgctcgcc gaatcggttg cggcccagct caaggacgtc ggcgtcgact
     1981 ccgtcgtcga ggtcgtggac caggcggcgc tcatcgacca ggccctcggc ggcaacttca
     2041 acatcatctt gtggcgtaac cacccgggcc tcgaccccga caccgagtac aactggtggc
     2101 attccgattc cccggtgaac ttcggcaaga ttaacgatcc tgcgatcgac gatgccctcg
     2161 atcggggccg ggtctcgacc gatccggctg agcgcaagac gatctacgaa gacctgaacc
     2221 gggcgttcgc caagggccgc tacaaccagt ggaactggta taccgagtgg gccgtcggag
     2281 cctcgacgaa ggtgcacaac atcaccgggt cgacgcttcc ggacggatcg gccaccccag
     2341 gggtgacgtg gggctggcat tccctcgccg agacctgggt cgatcagtag gtaaggcgag
     2401 caacggggtc tccgaggcca ctcctcggag accccgtttc tccatcggtg gcgattcttc
     2461 gccgcccacg gttcgagcgt gaggaacccc acatgagact tctgggacgt cgcttcctac
     2521 aactcatcgt cgtcatcttc ttgttgtcac tcgtcgcgtt cttctcgttg cgtttgatcc
     2581 ccggcgatcc cgcggagatc atcggcctcg gcgccgaccc cgcgacgatc gaacagatcc
     2641 gcgacgacat cggactgaac gagtcggtgg tcacccagta cacgcgctgg gcgggcaact
     2701 tcgttcgagg cgacctcggc aagtattacc acgcagccgg cgacccggcc gccgatgtcc
     2761 ggcgcacgtt gccgatctcg ctgcaactca tggtgtacac gcagatcgtc gcgttggcct
     2821 tcgccatccc gctgggggtt ttcgccgcat accgcgccga ccgcctcttc gatcggatgt
     2881 cgaacatcgg agcgttcttc gccatttcca tcccggcctt cgtcctggcc tacctcgcca
     2941 agcagtactt cgtcttcaaa tggaacctgc tgcccgaacg cggatggcca cccctcagcg
     3001 agggggtcgg cgatcatttc cgccatgcgg tgttgccggt cgccgtcatg tcggtggccc
     3061 aaatcgcggt ctactaccgc ctgttgcgta gcgacatgat ctccacgctc caggaggact
     3121 acatcaccat ggctcgggcg aagggcctcg gatcgcaacg catcctgttt cgccatgcac
     3181 tacggccgag ctcgctcacc ctgctcaccg tggcaggcct caacgtcggg tccatgatct
     3241 ccacggcggt catcgtggag aagatcttcg gcatcgacgg aatggggctc aagctcgtga
     3301 cggcggtgtt cagccgcgaa tacatcgcgg cgcagagctt catcgccgtg atcggcatct
     3361 tgttcgtgct catcaatttc ctggtcgacc tgctctacac cgtcctcgat ccccggatcc
     3421 gacatgcctg acacgatgac tgaccctgca gccgagaccg ccgtcgcggt caaagcctcg
     3481 aaacagaaac tcggtgcggt cggctgggtg tgtgccgcct ggctcatcct gctcgtcctg
     3541 gtgtccctct tcggcgggct cctcaaggcc acaccgatcg aggagggatc acgagcgctg
     3601 cgggcgtgcg gcgatggcag cgg
//
LOCUS       DAOOMA010000235         3614 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_31167_length_3614_cov_3.497612, whole genome shotgun sequence.
ACCESSION   DAOOMA010000235 DAOOMA010000000
VERSION     DAOOMA010000235.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 3614)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3614)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3614
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31167_length_3614_cov_3.497612"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1067)
                     /locus_tag="PLP95_07260"
     CDS             complement(<1..1067)
                     /locus_tag="PLP95_07260"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HPB45635.1"
                     /translation="MLSNVVSRANAAFRQPFTLADFSDRVAAMWGPRQMVDEAGGYRA
                     TFTEAGAMVRVLSEVIAASCAPNERVVLAVPNSYDQFLLCLAVSRAGCLPVPVNSQMK
                     PAEIDHVVADSEAALVVTSLDQLDEMALDLRDRFGSIAAIEPATPAHDDVAALFYTSG
                     TTGKPKGAALTHKALVGSAGLATLAPIGFGDVEVVVALPVAHIFGFAMLVASAAAGVR
                     VHFMSRFRAGPVLDAIEERRSAAFVGVPAMYRMMLEAGADHRDLKSIRLWISGADALP
                     DGLASTFKKLGAAAELPMVGPVGEAAFVEGYGMVEIGGGAALRVSLPLVPERFGGNLA
                     LRMPGYRFRVVDEDGKVLGHGR"
     gene            complement(1074..3335)
                     /gene="pcrA"
                     /locus_tag="PLP95_07265"
     CDS             complement(1074..3335)
                     /gene="pcrA"
                     /locus_tag="PLP95_07265"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012223995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006268 - DNA unwinding involved in DNA
                     replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase PcrA"
                     /protein_id="HPB45636.1"
                     /translation="MSTAELLDGLNAAQYEAVTHQGGPLLVVAGAGSGKTRVLTHRIA
                     WLIEEQRVSPFEILAITFTNKAAAEMKHRVASLIGPVADRMWVSTFNSACVRILRRDG
                     GRLGYPSNFTIYDQTDAVRLVTYILRDLDIDPKKLSPRSVHAAISNAKNRGIGVDDYV
                     EQAQVIIERRIADVYREYQTRLLRAGAMDFDDLLGQAVELMKRCPDVLEMYQTRFRHV
                     LVDEYQDTNPVQNELVMLLGREHRNVFAVGDGDQSIYAFRGADLTNILEFERAFPDTT
                     VVVLNQNYRSTQTILDAANSVIANNESRKPKELFTDGAAGEAIVRYTADDESDEAQWV
                     CSEIAKLRDSGEFRYGDIAVFYRTNAQSRVIEDGLFRAGLPYKVIGGTRFYDRREVKD
                     ALAYLRVAVNPDDEVSVKRVMNVPKRGIGESSVSRLDAYARSNRISFYEALRDAPAAG
                     VSGRALKGLETFTKIIEDARHALDRAPGDVLESLLDDSGYVDELAAERTIESEGRLEN
                     LSELVGAAREFETIDEFLEQVSLVSDTDQIDDDESTVTLMTFHGAKGLEYPAVFLVGL
                     EDGIFPSMRSLDEPHQLEEERRLAYVGITRARERLFLSSANARMLHGTTQYNPPSRFL
                     DEIPARLVTELGERRGRRSMRFGSSWDGDSGYGSSSSSGYGSSGYGSAGFGSSGSAGN
                     PLAARSSAPAPSDAQNAGLKVGDDFRHAEWGEGVILDIRGGGDDTQATVRFRETGNEK
                     VLLLKWAPITKIG"
     gene            complement(3399..>3614)
                     /locus_tag="PLP95_07270"
     CDS             complement(3399..>3614)
                     /locus_tag="PLP95_07270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HPB45637.1"
                     /translation="PHSSRSLRSMTSRISEAELVPGDLVFGGNPVHHVGIYVGNGQMV
                     HAPQTGDVVKVSSIYRTSKPVSFGRLP"
BASE COUNT          638 a         1232 c         1135 g          609 t
ORIGIN      
        1 cgcccgtggc ccaacacctt gccgtcctcg tcgacgaccc ggaaccggta gccgggcatt
       61 cgcagcgcga ggttgccgcc gaaccgttcc ggtaccaacg gcagcgacac ccgaagcgcg
      121 gctccaccgc cgatctcgac catcccgtag ccctcgacga aggccgcttc gcccaccggg
      181 ccgaccatgg gaagctccgc cgccgccccc agcttcttga acgtcgacgc cagcccgtcg
      241 ggcaacgcgt cggcgcccga gatccacagt cggatcgact tgaggtcgcg gtgatcggcc
      301 ccggcttcga gcatcatgcg atacatggcc ggaaccccga cgaacgccgc agatcggcgc
      361 tcctcgatgg cgtcgagcac cggaccggcc cggaaccggc tcatgaaatg gacccgcacc
      421 cccgccgccg cgctcgccac gagcatcgca aacccgaaga tgtgggcgac gggcagcgcg
      481 acgacgacct cgacgtcgcc aaacccgatc ggcgccagcg tcgccagccc cgccgatccg
      541 accagcgcct tgtgggtgag cgcggcgccc ttcggcttac cggtcgttcc cgaggtgtag
      601 aacagcgccg ccacgtcgtc gtgtgcaggg gtggccggct cgatggcggc gatggagccg
      661 aatcggtcgc gcaggtcgag ggccatctca tcgagttggt cgagcgaggt caccaccagc
      721 gcggcttcgc tgtcggcgac cacgtggtcg atctcggcgg gtttcatctg cgagttcacc
      781 ggaaccggca ggcacccggc cctcgacacg gcgagacaca acagaaactg gtcataggag
      841 ttcggcaccg cgagcaccac ccgctcgttc ggggcacagg aggcggcgat gacttcggac
      901 aagacgcgaa ccatcgcgcc cgcctcggtg aacgtcgccc ggtatccgcc ggcctcgtcg
      961 accatctgac gcggccccca catcgccgcg acccggtcgg agaaatcggc cagggtgaac
     1021 ggttgccgga aggcggcgtt ggctcgtgaa acgacgttgg acagcacgtt gagttatccg
     1081 atcttggtga tgggcgccca cttcaacagc agcaccttct cgttgccagt ttcgcgaaaa
     1141 cgcacggtgg cctgggtgtc gtcgccgcct ccacgaatgt cgagaatgac cccctcaccc
     1201 cactcggcgt ggcggaagtc gtcgccgacc ttgagcccgg cgttttgcgc atcgctgggc
     1261 gccggcgccg acgaacgagc cgcgagcgga ttgcccgccg accccgacga gccgaaccca
     1321 gccgaaccgt agccggagga gccgtaaccc gaggagctgg aggagccgta accggaatcc
     1381 ccgtcccacg acgacccgaa ccgcatcgaa cgacgaccgc gccgctcgcc aagttcggtc
     1441 accaagcgtg ccggaatctc gtcgaggaag cgcgagggcg ggttgtattg ggtcgtgccg
     1501 tgcagcatcc gggcgttcgc cgaggacagg aacagccgct ctcgcgcccg ggtgatgccg
     1561 acgtaggcaa gacgccgctc ctcctcgagc tggtgtggtt catcgaggct tcgcatcgac
     1621 gggaaaatgc cgtcctccag acccacgaga aacaccgccg gatactccag acccttggcc
     1681 ccgtgaaaag tcatcagtgt gaccgtcgat tcgtcgtcgt cgatctggtc ggtgtcggac
     1741 accaggctca cctgttcgag aaactcgtcg atcgtctcga actcgcgtgc cgccccgacc
     1801 agttccgaca gattctccag acggccctcc gattcgatgg tgcgctcggc ggcgagttca
     1861 tcgacatagc cgctgtcgtc gagcagcgat tccaacacgt cgcccggggc gcgatcgagc
     1921 gcgtggcgag cgtcttcgat gatcttcgtg aatgtctcga gccccttcaa cgctcgcccg
     1981 ctcacccccg cggccggagc gtcgcgcagc gcctcgtaaa agctgatgcg gttgcttcgg
     2041 gcgtaggcat cgaggcgact caccgagctt tcgccgatgc cgcgcttggg cacgttcatg
     2101 acgcgtttga cgctcacctc gtcgtcggga ttcaccgcga cccgcaggta ggcgagcgca
     2161 tctttcacct cgcgacggtc atagaaccgg gtgccgccga tgaccttgta gggcagtccc
     2221 gctcgaaaca acccgtcctc gatcacacgc gactgggcat tcgttcggta gaacaccgcg
     2281 atgtcgccgt atcggaactc gcccgagtcg cgcagcttgg cgatctccga acacacccac
     2341 tgggcctcgt cggactcgtc gtcggccgtg taacgaacga tcgcttcgcc cgctgccccg
     2401 tcggtgaaca gttccttcgg cttgcggctc tcgttgttgg cgatgaccga gttggcggca
     2461 tcgagaatcg tctgggtcga gcggtagttc tggttgagca ccaccacggt cgtgtcgggg
     2521 aacgcacgct cgaattccaa gatgttggtc aggtctgcac cacgaaacgc gtagatcgac
     2581 tgatctccgt cgccgaccgc gaacacgttg cgatgctcgc gccccaacaa catgaccagt
     2641 tcgttctgca ccgggttggt gtcttggtat tcgtcgacga gcacatgacg aaaacgcgtc
     2701 tggtacatct ccaacacgtc ggggcaccgc ttcatcaact cgaccgcctg gcccaacaga
     2761 tcgtcgaagt ccatcgcacc cgcccgcaac agccgcgtct ggtattcccg atagacgtcc
     2821 gcgatgcgcc gctcgatgat gacctgggcc tgttcgacgt agtcgtcgac cccgatgccg
     2881 cggttcttgg cattcgagat ggcggcgtga accgaccggg gcgacagctt cttcggatcg
     2941 atgtcgaggt cgcgcagaat gtaggtgaca agacgcaccg catcggtctg gtcgtagatc
     3001 gtgaagttcg acggataccc gaggcggccg ccgtctcgac gcaggatccg cacgcacgcc
     3061 gagttaaatg tcgacaccca catccgatcg gccaccggac cgatcaggct ggcgacacga
     3121 tgcttcatct cggccgcggc cttgttcgtg aacgtgatcg ccaggatctc gaacggcgac
     3181 acccgttgtt cctcgatgag ccaggcgatg cggtgagtga gcacccgggt cttgcccgaa
     3241 cccgctccgg cgacgaccag cagcggcccg ccctggtggg tgacggcctc gtattgcgcg
     3301 gcgttcaggc cgtcgagaag ttccgcagtc gacatctccc taacctagtt ggcacccctg
     3361 acagccggtt cagggccggt gcctgggcgg gttccgggtc agggaaggcg gccgaagctg
     3421 accggcttgg aggtgcggta gatgctcgac accttgacca cgtcaccggt ttgcggggcg
     3481 tgcaccatct gcccgttgcc gacatagata ccgacgtgat gcaccgggtt gccgccgaac
     3541 accaggtctc ccggcacgag ttcggcctcg gagatgcggc tggtcatcga acgcagcgat
     3601 cgagagctgt gtgg
//
LOCUS       DAOOMA010000236         3612 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_31185_length_3612_cov_3.764689, whole genome shotgun sequence.
ACCESSION   DAOOMA010000236 DAOOMA010000000
VERSION     DAOOMA010000236.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 3612)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3612)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3612
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31185_length_3612_cov_3.764689"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(114..1214)
                     /locus_tag="PLP95_07275"
     CDS             complement(114..1214)
                     /locus_tag="PLP95_07275"
                     /inference="COORDINATES: protein motif:HMM:NF025244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4202 family protein"
                     /protein_id="HPB45638.1"
                     /translation="MKVRTPAWLGAAIEAIDAANAQDPTLVDDAGVARPKELVHAEHM
                     TAWLDRLDPDASPAQRLAARAHHFRRWTVPRSEFPDGRAGYLRWRKQARQRHAEEVSA
                     LLSDHGVPAEVIDEVGRIVAKAPRRSERGDSGLKLTASEPHSVDIDPPQADSLEAEVQ
                     THEDCLCLTFFELQGLDTAARLGDKAAEVVAKTLEKMSAHGRSHLDRADLDPAVRELI
                     SSAQSTVEIIERLADADQRFTAQRRRIVSLLLGAGRPVSMPELLDADPDLAQSSVYRN
                     LVVLEQAGVVLRVVTGSEFTRYELAEDLTGHHHHHLVCVVCGKVDDVTVPDALERQLE
                     ATLSALAEAHGFAIDDHRLDALGRCAACIATA"
     gene            complement(1211..1432)
                     /locus_tag="PLP95_07280"
     CDS             complement(1211..1432)
                     /locus_tag="PLP95_07280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB45639.1"
                     /translation="MADGLDLDAMLARFQARAESVKQRQIPPVEGEARRAFVEQSKLD
                     YQDFAIIANSDAELVDGVLTFRIDLRPKG"
     gene            complement(1530..2393)
                     /locus_tag="PLP95_07285"
     CDS             complement(1530..2393)
                     /locus_tag="PLP95_07285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB45640.1"
                     /translation="MGKRAAVAAIGAVVAAGSWATAAHASVLTVAATLQDGGAPDATA
                     VDPDPAAERLQLISYSLVGLAVLIIVMTVIYWVMTRPVRVTSGDLAGQAPVVGGSSTE
                     GASIDLTEAPEPTGPRVDAPLPNPAALTPPPAAVPAAAPAPAGAAAAAAAPAPAPAGA
                     AALAPAPAAAAAALTSALAAIPDMTPTMIRFDDPPVQRVGRQLPAPGAPNFEQAARDN
                     TARREAVASARRADQRPPRTLRTAQPDPDRPRPDPSRRRRQDSPRAVADGEILDRPTE
                     TVRLEPPDGNR"
     gene            complement(2542..3324)
                     /locus_tag="PLP95_07290"
     CDS             complement(2542..3324)
                     /locus_tag="PLP95_07290"
                     /inference="COORDINATES: protein motif:HMM:NF013328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase family
                     protein"
                     /protein_id="HPB45641.1"
                     /translation="MPELIEAEIYRGALAATIGRRIAGVEILTPSFFHSGDLGDDTAV
                     STLIGSRIEAVERHGKVVIMRTDTGHDLSVRFGMTGRVIVDGDAPIDALEYGPRRDDE
                     AWDRLRLHLDDGFAVRFNDPRRFGSVSLDADLHALGPDIVSATVAQWRAALVGRRPVK
                     AVLLDQARLAGLGNLLCDEILWRSALAPTRPADSLDREAFVELRRTARATIRLLSRRG
                     GSHRGDLQEQRHVGGRCPADGTGLRREVVAGRTTYWCPTHQH"
     gene            complement(3312..>3612)
                     /locus_tag="PLP95_07295"
     CDS             complement(3312..>3612)
                     /locus_tag="PLP95_07295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012230908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PLP-dependent transferase"
                     /protein_id="HPB45642.1"
                     /translation="GGLLTFDLRGGATAGQTFVESTRICQLATSLGGPETLVTHPRTT
                     THAALLPAEAQAAGIHDGTVRVSVGLEHVDDLIADISDAIDAAQAAQAAQAACQN"
BASE COUNT          550 a         1207 c         1299 g          556 t
ORIGIN      
        1 cgttctccga taaccagtgg tcgtgatcac gaccactggt tatcggagaa cgagccgggg
       61 ggtcacgagc ctgggggtca cgaggcgggc ggtagcgagc ctgaaggtcg cggctaggcg
      121 gtggcgatgc aggcggcgca gcggccgagc gcatcgagtc gatggtcatc gatcgcgaaa
      181 ccgtgcgcct cggcaagcgc actgagggtc gcctcgagct gacgttccag cgcgtcgggc
      241 accgtcacgt cgtcgacctt gccgcacacg acacacacca ggtgatggtg gtgatgtccg
      301 gtgaggtcct cggcgagttc gtagcgggtg aactccgacc cggtcaccac ccgaagcacc
      361 actccggcct gttcgagcac gacgaggttt cgatacaccg agctctgggc gagatcgggg
      421 tcggcgtcga gcagctcggg catcgacacc ggccggccgg cgccgagcag caatgagacg
      481 atgcgacgcc gctgagcggt gaagcgttga tcggcgtctg cgagccgctc gatgatctcc
      541 acggtcgact gggccgagga gatgagctca cgcacagcag ggtcgaggtc ggcccgatcg
      601 agatggctgc gcccatgagc gctcatcttt tccaacgtct tggccaccac ctcggcggct
      661 ttatcgccca ggcgcgctgc ggtgtcgagg ccttgcagct cgaagaacgt gagacacaag
      721 cagtcctcgt gggtctggac ctccgcctcc agcgagtccg cctgaggcgg gtcgatgtcg
      781 accgagtgcg gttctgacgc ggttaacttc aggcccgaat cgccgcgctc actgcgcctg
      841 ggcgctttgg cgacgatgcg gccgacctcg tcgatcacct cggcgggcac cccgtggtcg
      901 gagagcagcg ccgaaacctc ctcggcatgt cgctgacgcg cctgtttgcg ccagcgcagg
      961 taccccgcac ggccatcggg aaactccgaa cgtggaacgg tccaacgtcg gaagtgatgc
     1021 gcacgtgcgg cgaggcgttg cgccggggag gcgtcggggt cgagtcggtc gagccacgcc
     1081 gtcatgtgtt cggcgtgcac gagttccttg ggccgggcga cgcccgcgtc gtcgaccagc
     1141 gtcgggtcct gagcgttggc cgcatcgatc gcctcgatgg ctgctccgag ccacgccggt
     1201 gtgcgcacct tcacccttta ggtcgaaggt cgatgcggaa cgtcaacacc ccgtcgacga
     1261 gttccgcgtc ggagttcgcg atgatcgcga agtcctggta gtcgagcttc gactgttcga
     1321 cgaaggcacg gcgggcctca ccctcaacgg gtgggatctg ccgctgtttc accgattcgg
     1381 ctcgggcttg gaatcgggcg agcattgcat cgagatcgag accgtctgcc atggctccac
     1441 cctatcggag cgcctcgcct cgacggtcgc ctcgacggtc gcccggccct gggtcggacc
     1501 cggacctacc cggacggacc cggacggact cagcggttcc cgtcgggcgg ctcgagtctg
     1561 accgtctcgg tcggacgatc gaggatctcc ccgtcggcaa cggcccgggg cgagtcttga
     1621 cggcgtcgcc gcgacgggtc ggggcgggga cgatcggggt ccggctgggc ggtgcgcagc
     1681 gtgcgcggcg ggcgttgatc ggcgcgccgt gcgctcgcaa ccgcctcgcg gcgagcggtg
     1741 ttgtcccgcg ctgcctgctc gaaattcggt gcgccggggg cgggaagttg ccgaccgaca
     1801 cgctgcaccg gcgggtcgtc aaaacggatc atcgtcggcg tcatgtccgg gatggcagcg
     1861 agcgctgagg tgagtgccgc cgcagccgca gccggagcag gagcaagagc agccgcgcca
     1921 gccggagcag gagcaggagc cgcagcagca gcagccgcgc cagccggagc cggtgccgca
     1981 gccggaacgg cagcaggcgg gggcgtcagt gcggcggggt tcggaagcgg tgcgtcgacg
     2041 cgggggcccg tcggctcggg cgcctcggtg aggtcgatcg aggcgccctc ggttgacgag
     2101 ccacccacca ccggggcctg tccggcgagg tcgccgctcg tgacccgcac cggacgggtc
     2161 atgacccaat agatgacggt catcacgatg atgagcaccg ccaaccccac gaggctgtag
     2221 ctgatgagct gcagtcgttc cgccgcaggg tcggggtcga cggcggttgc gtccggggcg
     2281 cctccgtcct gaagggtcgc cgcaaccgtc agcacggatg catgtgcggc cgtcgcccac
     2341 gatcccgccg ccaccacagc gccgatggcg gccacagctg ctcgcttccc caagcctcgc
     2401 acgatccgtc cgatctacct ttcgccgcga accggtgttc tcgacgcagc gaatcttact
     2461 gaccttcgcc atcgaattcg gtcacccgga cctgcgaatc tgctgcggcg acgaccatgt
     2521 ggccaccgcg gcaccgtcgg gtcagtgttg atgggtcggg caccagtagg tggtgcgccc
     2581 ggcgacgacc tcccggcgaa gcccggtgcc gtccgccgga cacctgccgc cgacatgacg
     2641 ctgttcctgc aggtcgcccc gatgcgaccc accccgacgc gagagcagcc gaatcgtcgc
     2701 tctggcggtt cgccgcaatt ccacgaaggc ctcccggtcg agcgagtcgg cgggacgggt
     2761 cggggccagg gcgctgcgcc acaaaatctc gtcgcacaac aggttgccga ggccggcgag
     2821 tcgggcctgg tccaacaaca cggccttgac cggacgacgg cccaccagcg cggctcgcca
     2881 ctgggcgacc gtcgcgctca cgatgtcggg gccgagggcg tgcaagtcgg cgtcgaggct
     2941 caccgaaccg aatcgtcggg ggtcgttgaa gcgaacggcg aatccgtcgt cgaggtggag
     3001 gcgcaggcga tcccatgcct cgtcgtctcg ccgggggccg tattcgagcg cgtcgatcgg
     3061 ggcgtcgccg tcgacgatca cgcggccggt catgccgaaa cgcaccgaga ggtcgtggcc
     3121 ggtgtcggtg cgcatgatga cgaccttgcc gtggcgttcg acggcctcga tacggctgcc
     3181 gatcagcgtg gagaccgcgg tgtcgtcgcc gaggtcgccg ctgtggaaga acgacggagt
     3241 caggatttcg accccggcga tgcgccgccc gatcgtggcc gcgagcgcac cccggtagat
     3301 ctccgcctcg atcagttctg gcacgccgcc tgcgccgcct gcgccgcctg tgccgcgtcg
     3361 atggcgtcgg agatgtcggc gatcaggtcg tcgacgtgtt cgagtcccac cgacacccgc
     3421 accgtcccgt cgtgaatacc cgccgcctgg gcctccgcgg gcagcagcgc ggcgtgggtg
     3481 gtggtgcgcg ggtgggtcac gagggtctcg gggccgccga gggaggtggc gagctgacag
     3541 atccgggtcg actccacaaa ggtctgtccg gccgtggcgc cgccgcgaag gtcgaaggtc
     3601 aacagtccgc cg
//
LOCUS       DAOOMA010000237         3611 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_31200_length_3611_cov_2.768841, whole genome shotgun sequence.
ACCESSION   DAOOMA010000237 DAOOMA010000000
VERSION     DAOOMA010000237.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 3611)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3611)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3611
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31200_length_3611_cov_2.768841"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..475)
                     /locus_tag="PLP95_07300"
     CDS             complement(<1..475)
                     /locus_tag="PLP95_07300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB45643.1"
                     /translation="MSGKTGRASSIRQVRGVGLRVAGNVAEFGGYVLLARSLGPSSFG
                     RISVLLVIVRFVALACDRGVAYAGTREVAGRGADSPAIWAWQRRRESWSLAGTALVAL
                     ATVAIGWPELAPMALGVFARGASREWIATGQNRLEVAAVPSLVHGAGIVAGVLAVD"
     gene            493..1251
                     /locus_tag="PLP95_07305"
     CDS             493..1251
                     /locus_tag="PLP95_07305"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HPB45644.1"
                     /translation="MDLNSVDVVILTWNDEPSMLDTAIRSALGSVGVEVSVHVVDNGS
                     EPAPLLAEDLQTDPRVRLIRSERNLGVARGRNLGIASGHNPYVLLLDSDAELRPDCAR
                     RLLDAVRTPVPGDVSFSAPVYAGQRPEESAGMAPTLRLKMARALNLRGDYQPVAVDGE
                     PTWEIDFAIGACQLFERSAWESIGGIDETYFYGPEDVDFCLRLKKSGRRLVQVAGAEC
                     VHPPRRRHKNLLTVRGAQHGVAVARHLWRHRGGV"
     gene            complement(1387..2499)
                     /gene="nadA"
                     /locus_tag="PLP95_07310"
     CDS             complement(1387..2499)
                     /gene="nadA"
                     /locus_tag="PLP95_07310"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020379524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HPB45645.1"
                     /translation="MTTATPISFRIQRSLDESYTEASDAELAARIEKAKATLGDRLFI
                     LGHHYQRDEVIRWADARGDSFKLASLAKARPEADYIVFCGVHFMAEAADILTGDHQQV
                     VLPDLNAGCSMADMADIDAVEEAWEDLAEHVDISRVIPITYMNSAADLKAFVGRNHGA
                     VCTSSNARAVLEWAFERGDQVLFFPDQHLGRNTGYQMGYDESHMRVWNPRFDLGGLTP
                     DDVTAKFLLWKGHCSVHQRFSVKHIEDFRAANPGALIVVHPECSHEVCDAADMVGSTE
                     YILRAVADAPEGSVIGVGTEIHMVNRMANETPNKTVASIDPLVCPCSTMFRIDGPHLA
                     WVLEELVEGRVPNRIVVDPEDAEWARVALQRMLDIT"
     gene            2668..>3611
                     /gene="uvrC"
                     /locus_tag="PLP95_07315"
     CDS             2668..>3611
                     /gene="uvrC"
                     /locus_tag="PLP95_07315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020379048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HPB45646.1"
                     /translation="MVTRPPAGSIPDAPGSYQFKDAEGRVIYVGKAKSLRQRLNNYFG
                     DPRLMHPRTAQMVATADSVEWMQVKTEVDALMLEYSLIKRHRPRFNVRLVDDKSYPFL
                     AVTVDDEWPRAMVMRGSKRKGVRYFGPYGHAYAIRETLDLLLRTFPIRTCSDSKFRTH
                     ERQGRPCLLFHIEKCSGPCVGEIDKPFYDLLVRELIEFLDGDTDHIVKRIEQQMHDAA
                     SDLEFELAARLRDRLAAVKRAIEKQEMVAARDEDLDVIGIAQDDLEAAVQVFYVWRGR
                     VVGRKGFVLDKVLALSDSEVVERVLEELYFEEPPLGVP"
BASE COUNT          609 a         1169 c         1232 g          601 t
ORIGIN      
        1 ggtcgacggc gagcactccc gccacgatgc cggcgccgtg aacgagggag ggcaccgcgg
       61 cgacctcgag gcggttctgg ccggtggcga tccattctcg gctcgctcca cgggcgaaca
      121 cgccgagcgc catcggtgcg agttcgggcc aaccgatcgc cacggtggcg agggcgacga
      181 gggccgttcc ggccagcgac cacgactccc gccgacgctg ccacgcccag atggccgggc
      241 tgtcggctcc tcggccagcg acctcgcggg ttccggcgta ggcgacgccc cggtcacacg
      301 cgagcgctac gaatcgaacg atgaccaaca ggaccgagat tcgtccgaac gaactgggtc
      361 cgagcgagcg cgccagcaga acgtacccac cgaactcggc cacattgccg gcgacgcgaa
      421 gcccgacacc gcgaacctgg cggatcgagg aagcccgacc ggttttgcca ctcacacctt
      481 tacgctaccg gtatggatct gaactccgtc gatgtcgtga tcctcacctg gaacgatgag
      541 ccgtcgatgc tcgacacggc gattcgctcc gcgctgggca gcgtcggggt cgaggtgtcg
      601 gtccacgtcg tcgacaacgg cagcgaaccc gcaccgctcc tcgccgagga cctgcaaacc
      661 gacccccggg tgcgcctgat ccggtccgaa cgcaaccttg gggtggctcg cggccgcaac
      721 ctcggcattg cgtcgggcca caacccctac gtgttgctgc tcgacagcga tgccgagtta
      781 cgacccgact gcgcgcgacg cctgctcgac gccgtacgga cccccgtgcc gggcgacgtg
      841 tcgttcagcg cgcccgtcta tgcgggccag cgccccgaag agtccgcagg tatggccccg
      901 acgttgaggt tgaagatggc ccgagcgctg aaccttcggg gggactacca acccgtggcg
      961 gtcgacgggg agccgacgtg ggagatcgac ttcgccatcg gtgcgtgtca actgtttgaa
     1021 cgctcggcct gggagtcgat cggcggcatc gacgagacct acttctacgg tcctgaagat
     1081 gtcgacttct gcctccgcct caagaagagc ggccgccgtc tcgtgcaggt ggccggcgcc
     1141 gagtgcgtcc acccgccgcg acgccgacac aagaacctgc tgaccgtgcg cggcgcccag
     1201 cacggagtgg ccgtcgcccg ccacctgtgg agacaccgcg gcggcgtctg acccgccccc
     1261 ggcgcccccg ccctcgttgg gcgttctgta ggtaatcgcg gttgtcatag caaccgcgat
     1321 tacctacaga acgggaatcg ggcgggcgcg cggcggggga atcgggcggg cggtggcggg
     1381 aacagctcag gtgatgtcga gcatccgctg gagcgcgacc cgggcccact cggcgtcctc
     1441 gggatcgacg acgatccggt tcgggacccg gccctcgacg agttcctcca acacccacgc
     1501 caagtgcggg ccgtcgatgc ggaacatggt tgagcacggg cacacgagcg ggtcgatcga
     1561 ggcgacggtc ttgttcgggg tctcgttcgc catgcgattc accatgtgaa tctcggtgcc
     1621 gacgccgatg accgacccct cgggggcatc ggccacggct cgcaggatgt actcggtgga
     1681 tccgaccatg tcggcggcgt cgcacacctc gtggctgcac tcggggtgga cgacgatgag
     1741 cgcccccggg ttcgcggccc ggaagtcctc gatgtgtttc accgagaaac gctggtgcac
     1801 cgagcagtgg cccttccaga gcaggaattt ggcggtgacg tcgtcggggg tgagcccacc
     1861 gaggtcgaag cgggggttcc acacccgcat gtgcgactcg tcgtagccca tctgatagcc
     1921 ggtgttgcgt ccgaggtgct gatcggggaa gaacagcacc tggtcgccgc gctcgaaggc
     1981 ccactcgagc accgccctgg cgttggaact cgtgcacacc gcgccgtggt tgcgcccgac
     2041 gaaggccttg aggtcggcgg cggagttcat gtaggtgatg gggatcacgc ggctgatatc
     2101 gacgtgttcg gccaggtctt cccaggcctc ttcgacagcg tcgatgtcgg ccatgtcggc
     2161 catcgaacat ccggcgttga ggtcgggcag cacgacctgt tggtggtcgc cggtgaggat
     2221 gtcggcggcc tcggccatga aatgcacgcc gcaaaagacg atgtagtcgg cctcggggcg
     2281 ggccttcgcg agggaggcga gcttgaacga gtcgccgcgg gcatcggccc agcggatgac
     2341 ctcgtcgcgt tgatagtggt ggcccaggat gaagaggcga tcgccgaggg tcgccttcgc
     2401 tttttcgata cgtgcggcga gttcggcgtc ggaggcctcg gtgtaggact cgtcgagtga
     2461 acgctggatg cgaaacgaga tcggggttgc tgtcgtcatg atgcctcctg acggtaggtg
     2521 ccgaccacgg tagtgctcag ccgcgggaca accggatccc atcgtttcgc attcccgaat
     2581 cgggacgaaa gcaattttgc ccgggcgacg tccgaaccgt gccgaaccgt gccgaggggg
     2641 ccgaaccgtg caggtagcgt tgggcccgtg gtcacccgtc ctcccgccgg ctccattccc
     2701 gacgcccccg ggagttatca gttcaaagac gccgagggtc gggtcatcta cgtcggcaag
     2761 gccaaatcct tgcgccagcg gctgaacaac tacttcggcg acccgcgcct gatgcacccg
     2821 cgcaccgcgc agatggtggc caccgcggac agcgtcgagt ggatgcaggt caagaccgag
     2881 gtcgacgcgt tgatgttgga gtactccctc atcaaacggc atcggccccg gttcaacgtg
     2941 cggctggtcg acgacaagag ctacccgttt cttgccgtca cggtcgacga cgagtggccg
     3001 cgggcgatgg tcatgcgcgg ttcgaaacgc aagggtgttc gctacttcgg cccctatggc
     3061 cacgcgtatg cgatccgcga gaccctcgat ctgctgttgc ggacctttcc gatccgcacg
     3121 tgtagcgact cgaagttccg gacccacgag cgccagggcc ggccgtgtct gttgttccac
     3181 atcgagaagt gctcgggtcc ctgcgtcggt gagatcgaca agccgttcta cgacctgttg
     3241 gttcgcgaac tgatcgagtt cctcgacggc gacaccgacc acatcgtcaa gcgcatcgaa
     3301 cagcagatgc acgacgccgc ttccgatctg gagttcgaac tggccgcccg gctgcgcgat
     3361 cgcctcgccg cggtgaagcg ggccatcgag aagcaggaaa tggtggccgc ccgcgacgag
     3421 gacctcgatg tgatcggcat cgcccaggac gacctcgagg cggcggttca ggtcttctac
     3481 gtgtggcgag gtcgcgtcgt tggtcgcaag gggttcgtgc tcgacaaggt tctggcgttg
     3541 agcgacagcg aggtcgtcga gcgggtgctc gaggagctgt atttcgaaga gccgccactg
     3601 ggagtgccga a
//
LOCUS       DAOOMA010000238         3600 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_31318_length_3600_cov_3.317630, whole genome shotgun sequence.
ACCESSION   DAOOMA010000238 DAOOMA010000000
VERSION     DAOOMA010000238.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 3600)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3600)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3600
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31318_length_3600_cov_3.317630"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..529)
                     /locus_tag="PLP95_07320"
     CDS             complement(<1..529)
                     /locus_tag="PLP95_07320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB45647.1"
                     /translation="MFEHDGASHHAGTDSGDADGWAVLAGAIDAGECVAAELAGVVEQ
                     VAAGIAALDTAVSNGAGEQQRQAIARELSLTAARINHVAQRLHVASVDLAQRQHVNVH
                     DRVPTMGYWLALHHGITRRDANRLLRANTAAERFEQVGAAFETGEITLGHLDAIATII
                     PSAYSGERLDAAIHAV"
     gene            complement(706..1494)
                     /locus_tag="PLP95_07325"
     CDS             complement(706..1494)
                     /locus_tag="PLP95_07325"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HPB45648.1"
                     /translation="MSRNIRRGPPEVLEHDGVVIDRYRPVVEGPDRPPIVIVHGTMDR
                     AAGFRRTIGHLPDREVLAYDRRGYARSGERPVASRLTDHLEDMAVVLSLVEEPALLVG
                     HSLGGVIALWALATPPLEHDVLGAVIWEAPMAWEPWYEPKGSDLMSLPESEAAESFMR
                     RMIGDPLWERLPESMRNQRRAEGAALQADLRHSAAAESVVRFDRISAPVLIGYGSRSS
                     DNHRRSAITMAESLNDARVVEIDGADHGIHLSKPATFAAFIESW"
     gene            complement(1539..2972)
                     /gene="pyk"
                     /locus_tag="PLP95_07330"
     CDS             complement(1539..2972)
                     /gene="pyk"
                     /locus_tag="PLP95_07330"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: protein motif:HMM:NF004491.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HPB45649.1"
                     /translation="MSRRTKIIATIGPASDDPAVIRRMIAAGADVFRIGLAHGSLGDA
                     IERYRRIRAVAADAGREIGILADLPGPKVRLASFVDDGAVLAVGGEVELCPGAESSTT
                     TSLGVDYGDLLADLHPGDNLALGDGTVRLQVTSVDASSARAVVTYGGTVQGRPGLHVP
                     SDRLGLATPTAEDLYKLDAFVEEGVDMVAVSFVRSAHDIRRVGTEPSPRGPLIVAKIE
                     TRAAVDNLEGIIEASGAVMVARGDLGIEMGIEDLPHIQKRIIRDCIAYGRPVITATQM
                     LESMITAGTPTRAEVSDVANAIFDGSSAVMLSAESAIGVDPVNSVATMARICAKADTE
                     FDFDGWAHRLRALRKFHSNDSDGRLTDAITGAAWRAATEVGASAIIAVSDTGFTVRSI
                     ARFRPSMPILGFTPNERTARQLCLSWGTTPLHDPVAANHEDLMTSLVIAARDHGDVRS
                     GDIVAVISGSQVNRAKATDQMRLVRVP"
     gene            complement(2999..>3600)
                     /locus_tag="PLP95_07335"
     CDS             complement(2999..>3600)
                     /locus_tag="PLP95_07335"
                     /inference="COORDINATES: protein motif:HMM:NF012656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HPB45650.1"
                     /translation="GDAEFNEVYFTDTRIADSMRVDEVGRGWAVALTTLMNERVSIGG
                     GTPPRNSGPIGQLLDLYRSGERGAVDRDQFMRLWSQAEINRLTNIRASQNRKLGTPGP
                     EGSVAKLAMAELNKAIYSFAVDLMGAAGMLYATYDFPPAKQAMFGDDLSVNFLRARAN
                     SIEGGTTEVMKNILGERVLGLPGDVRVDKDVPWSRVARS"
BASE COUNT          615 a         1208 c         1183 g          594 t
ORIGIN      
        1 gaaccgcgtg gatcgcagca tcgagacgtt cacccgaata ggcgcttggg atgatcgtcg
       61 cgatggcatc gaggtgtccc aaggtgatct caccggtttc aaacgcggca ccgacctgtt
      121 cgaaacgttc agcggccgtg ttggcgcgta ggagccggtt ggcgtcgcgg cgggtgatgc
      181 cgtgatgtag agcaagccag tagcccatgg tggggacacg gtcatgcaca ttgacgtgtt
      241 gacgttgcgc cagatcgaca ctggccacat gaaggcgttg agcgacatgg ttgatccgcg
      301 ctgcagtcaa cgacagttca cgggcgatcg cttgacgctg ttgttcacca gcaccgttcg
      361 agaccgcagt atcgagcgct gcgataccgg cagccacctg ttcgacaaca ccggccaatt
      421 cggccgcaac acactccccg gcatcgatgg ccccagcgag cacggcccag ccatcggcgt
      481 cgccgctgtc ggtgccagcg tggtgtgatg ccccgtcatg ctcaaacatg tagtcataat
      541 agcgaacagg tgttcgtctc gcaagccgaa aaactggaat gttcaaacga gaacgacacg
      601 ttgttgccgc gaaccccgtg ccacctccac gcacacccac ctccctgcgc acccacctcg
      661 gcgacgtgac gccgcggagc cagatggagc cagatggagc gggggtcacc agctctcgat
      721 gaacgcggcg aaggtcgccg gtttcgacag gtggattccg tgatcggcac cgtcgatctc
      781 gacgacgcgg gcatcgttga gcgattcggc catggtgatc gccgagcggc ggtggttatc
      841 gctcgaccgg ctgccataac cgatcaacac cggtgccgag atccggtcga accgaaccac
      901 cgattccgca gcggcgctgt ggcgaagatc ggcctgcagc gcagccccct cagcccgacg
      961 ttggttgcgc atggactcgg gcagccgctc ccagagtggg tcgccgatca tccgacgcat
     1021 gaacgactcg gccgcctcgg attcgggtaa cgacatcagg tcgcttccct tgggctcata
     1081 ccacggctcc cacgccatcg gcgcctccca gatcaccgcg cccaacacgt cgtgttcgag
     1141 cggtggggtc gccagcgccc aaagcgcgat cacgccgccg aggctgtggc cgacgagcaa
     1201 cgccggctcc tcgaccaggg agagcacgac cgccatgtcc tcgaggtgat cggtgaggcg
     1261 ggatgcgacc ggacgctcgc ccgaacgtgc ataaccgcgg cggtcatagg ccagcacctc
     1321 gcggtccggc agatgcccga tcgtgcgccg aaagccagcc gctcggtcca tggttccgtg
     1381 cacgatcacg atcggcggcc gatcgggccc ctccacgacc ggtcggtagc ggtcgatcac
     1441 caccccatcg tgttcgagga cctcgggcgg cccgcgacgg atgttacggc tcacgctcga
     1501 ccccggccca cgttcgaacc cggcccacgc gccagcgcct agggcacccg gaccagacgc
     1561 atctggtcgg tcgccttggc ccggttcacc tgggaaccgc tgatgacggc gacgatatct
     1621 cccgagcgga catcgccgtg gtctcgggcc gcgatcacca ggctcgtcat gagatcctcg
     1681 tgattcgcgg ccacggggtc atgaagcggc gtggtgcccc aactcagaca cagctgccgg
     1741 gcggtgcgtt cgttcggcgt gaacccgagg atcggcatgc tcgggcggaa ccgggcgatc
     1801 gaccgcacgg tgaacccggt gtcgctgacc gcgatgatcg ccgacgcgcc gacctcggtc
     1861 gccgcgcgcc acgccgctcc ggtgatcgca tcggtgaggc gaccgtcgct gtcgttgctg
     1921 tggaacttcc gaagcgcccg caggcgatgc gcccacccgt cgaaatcgaa ctcggtgtcg
     1981 gccttggcac agatgcgcgc catcgtggcc accgagttca cggggtcgac gccgatggcc
     2041 gactctgcgc tgagcatcac cgcggaggag ccgtcgaaga tggcgttcgc cacgtcggag
     2101 acctcggccc gggtcggggt gccggcggtg atcatcgatt cgagcatctg ggtcgccgtg
     2161 atcaccggcc gtccgtacgc gatgcagtcg cggatgatgc gcttttggat gtggggcagg
     2221 tcctcgatac ccatctcgat gccgagatcg cctcgggcca ccatgaccgc gcccgatgcc
     2281 tcgatgatgc cttcgagatt gtcgaccgcc gcacgagttt cgatcttcgc cacgatgagc
     2341 gggccccgtg gcgacggttc ggttccgacc cggcggatgt cgtgggccga ccgcacgaag
     2401 ctgaccgcga ccatgtcgac gccctcctcc acgaaggcgt cgagtttgta caggtcctct
     2461 gcggtcggcg tggcgagacc gagacggtcc gacgggacgt gcaaaccggg acggccttgc
     2521 accgttccgc cgtaggtgac gaccgcccgc gccgaggatg cgtcgaccga ggtgacctga
     2581 aggcgaacgg ttccgtcgcc gagggccagg ttgtcgccgg gatgcaggtc ggcgagcaga
     2641 tcgccgtagt cgacccccag cgaggtggtg gtggacgact ccgcaccggg gcacaactcg
     2701 acctcgccac ccaccgccag aacggcaccg tcgtcgacga acgaagcgag ccggactttg
     2761 ggtccgggga ggtcggcgag gatgccgatc tcccggccgg cgtccgccgc cacggcacgg
     2821 attcgccggt accgctcgat ggcgtcgccg agcgaaccgt gggcgaggcc gatgcgaaag
     2881 acgtccgctc cggcggcgat catgcgccgg atcaccgccg gatcatcaga ggcaggtccg
     2941 atggtggcaa tgatcttggt gcgtctcgac atgacgtcga atctaccgac ccggagactc
     3001 agctccgcgc cactcggctc caggggacgt ctttgtcgac ccgcacgtcg cccggcaggc
     3061 cgagtacccg ttcacccagg atgttcttca tgacctcggt ggtgccaccc tcgatggagt
     3121 tcgcacgggc gcgcaggaaa ttcacgctga ggtcgtcgcc gaacatcgcc tgcttcgccg
     3181 gcgggaagtc ataggtggcg tacaacatcc cggcggcgcc catcaggtcg accgcgaagg
     3241 agtagatcgc cttgttgagt tcggccatcg ccaacttggc gaccgacccc tccgggccgg
     3301 gggtgccgag ctttcgattc tgtgacgccc ggatattggt caggcggttg atctcggctt
     3361 gggaccacag cctcatgaac tggtcgcggt cgaccgctcc gcgctcgccg gagcggtaca
     3421 ggtcgaggag ttggccgatc ggtccgctgt tgcgcggcgg tgtgccaccc ccgatcgaca
     3481 cccgttcgtt catcagggtc gtgagcgcca ccgcccagcc gcggcccact tcatcgacgc
     3541 gcatcgaatc ggcgatccgt gtatcggtga aatagacctc gttgaactcg gcgtcgccgg
//
LOCUS       DAOOMA010000239         3591 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_31441_length_3591_cov_3.262443, whole genome shotgun sequence.
ACCESSION   DAOOMA010000239 DAOOMA010000000
VERSION     DAOOMA010000239.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 3591)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3591)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3591
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31441_length_3591_cov_3.262443"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            98..295
                     /locus_tag="PLP95_07340"
     CDS             98..295
                     /locus_tag="PLP95_07340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB45651.1"
                     /translation="MRTLRISCDDCIMQATSACDDCVVALILSREPDDAVVLSMEEHL
                     VLRRLAASGLVPEVRYQCESL"
     gene            626..1819
                     /gene="queG"
                     /locus_tag="PLP95_07345"
     CDS             626..1819
                     /gene="queG"
                     /locus_tag="PLP95_07345"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HPB45652.1"
                     /translation="MLDGSDLSELFEVGRAAGLDLVGATRAAAWGHTRTQLERRRNLG
                     LNADMAFTYKNPPRSTDATRVVKGAATLIVGARSYLQTDDAPPDPSIEVAAQVARYAT
                     ADHYGQLAASLERVAEALRARGARAVVVLDRNDIVDREAAWRCGIGWYGKNSLLLAPG
                     FGSWIVLGSVVTNARLNPDTAPVPTPVGDHCGTCRRCVDACPTGAIVADGVIDAGRCL
                     SWLLQARGGFPVEHRVALGDRIYGCDDCQTACPPNRAVELRARRLESSDDVGSGPHRS
                     IASPGRWMDAMELLNLDDEALLDRVGRWYIADRNPDLVRRNLLIVIANSAPADNASVR
                     SVLEQFDSGADPMLSEHATWALNSLDDRIGSTIGSTIGAGSGTTTDQTTTMESDPPPA
                     TTRAP"
     gene            1816..3039
                     /locus_tag="PLP95_07350"
     CDS             1816..3039
                     /locus_tag="PLP95_07350"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HPB45653.1"
                     /translation="MNESGAANHLLVTNDFPPKVGGIQSYLWELWRRLPPAATTVLTT
                     PYSGSEAFDAVSPIRIVRTPDKVLLPHPGLARRINRLAQEVDASMVVLDPVLPLGALA
                     PQIERPIAYVLHGAEITVPGRLPGSAQLMGSLLRRADLVIAAGGYPLAEAQRCAGRRL
                     NSVVIPPGVDTERFIPISHDERRTTRRRLGLPEDARIVTAVSRLVPRKGFDMVIRAAA
                     RLAPARPDLLVAIAGSGRDEARLKRLATSLGAPVAFMGRIPDEDLAAFHACGDVFAML
                     CHDRWFGLEQEGFGIVFLEAASCGVPQLAGRSGGSHEAVEHGVTGSVVDDPRDVVDVA
                     TGLAHLLDHPDPAMARESRRRAVAEFSYDLLAARLAQALGCGPATSSAEISGEISAEP
                     SGVTDGDSAPEAVGR"
     gene            3036..3572
                     /locus_tag="PLP95_07355"
     CDS             3036..3572
                     /locus_tag="PLP95_07355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB45654.1"
                     /translation="MNVADERAKAQALAKQRRSANAAALEEYFAEHPTGRWIVLTSWA
                     STGVFVLTTVVATVVNNREARLVAALLSLALFSISLVLHLVALWLGAQRSRRDEMTMV
                     GWWFLSESAPKSTRAHLWGALLMQIVVSCVCAAIRFETSIAFGVLVPMLGLSFLGLWG
                     AMYGMFAPRRASTGGSVR"
BASE COUNT          546 a         1188 c         1221 g          636 t
ORIGIN      
        1 acctcatggg cactctcggt ctgggtcgaa gcccttctcg gccaacccta aaaacgcggc
       61 aaccctcgct gtcggtggtt gtgtggatca tggcgccatg agaactctgc gaatcagctg
      121 tgacgactgc atcatgcaag cgacgtcggc ctgcgacgat tgtgtcgtcg ccttgatcct
      181 gtcgcgcgag cccgacgacg ccgtggtgtt gagcatggag gagcatttgg tgcttcgccg
      241 cctcgccgcc tcgggcttgg ttccagaggt gcgctaccag tgcgaatctc tgtgacgctg
      301 tccgagtccg tgccgaccag gggtgtagct accggttgac ctccgaggcg ggtcacctcg
      361 gaatcgacca agagacacca atagactcgg ggcgatgccg gatccacccg tggctcagac
      421 tggcgatccg cctgaacctg cgggaccgac cggagtcacg tccgtcacgt ccgccaaggc
      481 gacccgtggc gctgattcac gcggtgagcc gaaacgctcg caagcctcac acgcaacgcc
      541 gccccaggtc agcggcgggg ccaggcgccg tggcggggtc cggcgcaccg gcgatgcccg
      601 gcgcgctagc ggatcccaac cggtggtgct cgacggcagc gacctttccg aactcttcga
      661 ggtcggccga gcggcgggac tcgatctcgt cggtgcgacc cgcgccgcgg cgtggggtca
      721 cacccgaacc caactcgagc gccgccggaa cctgggcctg aacgcggaca tggccttcac
      781 ctacaagaac ccaccgcgca gcaccgatgc cacgcgggtg gtgaagggcg cagcgaccct
      841 catcgtcggg gctcgcagct acctccagac cgatgatgcg cccccggacc cttcgatcga
      901 ggtcgcggcg caggtggcga ggtacgcgac ggccgaccat tacgggcaac tcgccgcatc
      961 cctcgaacgg gttgccgaag cgttgcgcgc ccgaggcgct cgggcggtcg tggtcctcga
     1021 ccgaaatgac atcgtcgacc gtgaagccgc gtggcgctgt gggatcgggt ggtacggcaa
     1081 gaactcgctc ctgttggctc cagggttcgg gagctggatc gtgttgggct cggtggtgac
     1141 caacgcgagg ctcaaccccg ataccgcacc ggtgccgacg ccggtcggcg atcattgcgg
     1201 cacatgtcga cgctgtgtcg acgcatgtcc gaccggagcg atcgtcgccg atggtgtcat
     1261 cgatgcgggc cggtgtctgt cctggttgct gcaggcccgc gggggctttc cggtcgaaca
     1321 ccgtgtcgcg ctgggagatc ggatctacgg ttgcgacgac tgccagacgg catgtccgcc
     1381 gaaccgtgcg gtcgagttgc gggcccgacg cctggagtcg tcggatgatg ttgggtccgg
     1441 accacatcgc tctattgcga gccctggacg atggatggac gcgatggagc tgttgaacct
     1501 ggacgacgag gcgctgctgg atcgcgtcgg tcgttggtac atcgcggacc gcaatcccga
     1561 cctcgtgcgt cgcaacctcc tgatcgtcat cgccaacagc gcccccgccg acaacgccag
     1621 cgtccgctcc gtcctcgaac agttcgattc cggtgccgac ccgatgctgt ccgaacatgc
     1681 aacgtgggcg ctgaatagcc tcgatgaccg aatcggctcc accatcggtt ccaccatcgg
     1741 tgccggatct ggaacgacga ccgaccaaac caccacaatg gaatccgatc cgccaccggc
     1801 gacgacgagg gccccgtgaa cgaatccggc gcagcgaacc acctgctggt caccaacgat
     1861 ttccccccga aagtgggcgg cattcagtcc tacctctggg agttgtggcg tcgactcccg
     1921 ccggccgcca ccacggtgct gaccaccccg tactcgggat cggaggcgtt cgatgctgtg
     1981 tcgcccattc ggatcgtgcg gaccccggac aaggtgctgc tgccgcatcc cggcttggcg
     2041 cgccgcatca accgcctcgc gcaggaggtt gacgcctcga tggtcgtgct cgatccggtg
     2101 cttccgctgg gcgcgctggc cccccagatc gagcgcccga tcgcgtacgt cctgcacggc
     2161 gccgagatca cggttcccgg tcgtctcccg ggcagcgccc agctcatggg gtcgctgctg
     2221 cggcgagcag atctggtcat cgccgccgga gggtatccgc ttgccgaggc gcagcgttgt
     2281 gcgggtcggc ggttgaattc ggtcgtcatt ccgccggggg tcgataccga acgcttcatc
     2341 ccgatttcgc atgacgaacg tcgcaccacc cgtcgccgtc tcggcctgcc ggaggacgca
     2401 cgaatcgtga ccgccgtcag tcgcctggtt ccgcgcaagg gtttcgacat ggtgattcga
     2461 gcggctgccc ggctcgctcc cgcccgaccg gatctgctcg tggccatcgc cggatccgga
     2521 cgtgacgagg cgcgcctcaa gcggctcgcg acatcccttg gcgcaccggt ggcgtttatg
     2581 ggccggattc ccgacgagga ccttgccgcg ttccacgcgt gtggtgacgt gttcgcgatg
     2641 ctgtgtcacg accgttggtt cgggctcgaa caagaagggt tcggaatcgt gttcctcgag
     2701 gcggcgagtt gcggcgttcc gcagctcgcc ggacgcagcg gcggaagtca cgaggccgtg
     2761 gaacacgggg tgaccgggtc ggtggtcgac gacccgcgcg acgtggtcga cgtcgcgacc
     2821 gggcttgcgc acctgctcga ccacccagac cctgcgatgg cgcgcgagtc gcgtcggcgt
     2881 gcggttgcgg agttctccta tgacttgctg gcggccaggc ttgcgcaagc gctcggctgt
     2941 ggcccagcga catcctcggc ggagatctca ggggagatct cggcggagcc ctcgggggtg
     3001 accgatggcg actcggcccc ggaggcggtg ggtcgatgaa cgtcgcggat gaacgcgcca
     3061 aggcccaggc cctggccaag cagcggcgtt cggcaaacgc ggctgcgctg gaggagtact
     3121 tcgccgagca cccgaccggt cgctggatcg tgctcacctc gtgggcctcg accggggtgt
     3181 tcgtgttgac caccgtcgtg gccacggtcg tcaacaaccg cgaggctcgc ctcgtcgccg
     3241 cactgttgtc cctcgcgctg ttcagcatca gtttggtact ccacctcgta gcgttgtggc
     3301 tgggtgcgca acggagccgt cgcgacgaga tgaccatggt cgggtggtgg ttcctgtccg
     3361 aaagcgcccc gaaatcgacg cgggcacacc tttggggggc gctactgatg cagatcgtcg
     3421 tcagctgcgt gtgtgcggcg attcgctttg agacctcgat cgcgttcgga gtgctcgtgc
     3481 cgatgctcgg cctgtccttt ctcgggctct ggggtgcgat gtacggcatg ttcgcgccgc
     3541 ggcgcgcctc gacggggggt tcggtgcgat gaggacgatc ctgttcaccg g
//
LOCUS       DAOOMA010000240         3586 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_31488_length_3586_cov_4.284622, whole genome shotgun sequence.
ACCESSION   DAOOMA010000240 DAOOMA010000000
VERSION     DAOOMA010000240.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 3586)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3586)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3586
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31488_length_3586_cov_4.284622"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..427)
                     /locus_tag="PLP95_07360"
     CDS             complement(<1..427)
                     /locus_tag="PLP95_07360"
                     /inference="COORDINATES: protein motif:HMM:NF017760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HPB45655.1"
                     /translation="MSAGGLRGATVALQHREFRIFWIAAFVSNTGGWMQSAGLPYVAY
                     QLTGTNGGVGATGFMQYVPVLIAGAVGGWLADSFDLRKLLILTQLAQACIAFALWVLV
                     AQGWATPTSLAAVAFLGGLASGLNIPVWQSFVGKLVPRSI"
     gene            727..1527
                     /locus_tag="PLP95_07365"
     CDS             727..1527
                     /locus_tag="PLP95_07365"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HPB45656.1"
                     /translation="MTDAEIRRGDVDEWGRSERARRLARFLYEPLYSSYFRVEWEGLE
                     KIPIDGGALLVANHAGAIPSDAPAIMHGIETQLRRPVYGLADNFFRTLPVVGTMWSRL
                     GGLPAHPDNAYRLLREQNQLVLVFPEGSKGPAKRMDERYRLARFGRGGFVEIAMRAGV
                     PIIPIAVVGAEESMPIVAKLPSLAGVFGAPYVPITANMLLFGPAGLFTYFPSKFKLRV
                     LDPIHFDVEPDLARYSKSRIMDESEKIRDEIQDALYEMLSKRRSVWFG"
     gene            1527..2714
                     /locus_tag="PLP95_07370"
     CDS             1527..2714
                     /locus_tag="PLP95_07370"
                     /inference="COORDINATES: protein motif:HMM:NF013530.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HPB45657.1"
                     /translation="MAPGSTYESVERSGERPAVTPPRGRRVLVTGLGSFWGGRIAQAL
                     EDDPCVEVILGLDTTEPTVELERTEYVRVDANYSILHKIVAAAKIDTIIHTFLIVDPT
                     IMTARAMHEVNVIGTMNLFAAASAPDSTVRNVIVKSSTAVYGCAASDPTWFTEDMEPS
                     APLRRPVERSLDQVESYVRDFAEDNPHVRVSLLRFSNVIGTEISTPLTRALELPLVPS
                     VLGFDPRFQFVHESDVIRSILYVLDHDLSGVYNVAGDGLLPWSEVVSLCGKRRLPLMA
                     RVTSLATLPLRQFGLSGLPEEYLNLLRYGRGVDNTKLKQSGFEYDYTSAQAIKAFIEG
                     LRLRRTVGSHSGEYRYERDVEQFFRHSPAVVREAPPYDRRKPSDATDEAIEATDDRID
                     SQR"
     gene            complement(2636..>3586)
                     /locus_tag="PLP95_07375"
     CDS             complement(2636..>3586)
                     /locus_tag="PLP95_07375"
                     /inference="COORDINATES: protein motif:HMM:NF025072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HPB45658.1"
                     /translation="ETLDQSNYDVCFVSHVDCWYPIEDLLTIPTIVDFDNLENLLTRT
                     IREAGPIIEPGASVRRRVAASARWVVASAFNLVDERRWDSVQRRLANSVDLAFVCSSL
                     DVERSGYPNTVVVPNGYERTFEPADHVELNDSTAPVFLFVGLMSYDPNSDAVRRFASA
                     VMPRIRRELPGARFRIVGRNPESVASLERLDGVEVVGAVDSLEAELRGADVAVVPLRA
                     GAGTRLKVVEAMANRLPMVTTSIGCEGIDIVDGVHAWIRDDDEGIAEACIEAVRNIDA
                     RRSVIDAAEARYEERYRWESIRSSVASMASSVASEGLRRS"
BASE COUNT          685 a         1246 c         1061 g          594 t
ORIGIN      
        1 tgatcgagcg cggcaccagc ttgccgacga acgactgcca caccgggatg ttcagaccac
       61 tcgccaaccc accgagaaac gcgaccgccg ccagactcgt gggggtcgcc caaccctggg
      121 cgaccaagac ccacagagcg aacgcaatac acgcctgcgc gagttgggtc aggatcagca
      181 gtttccgcag atcgaacgag tcggcgagcc acccgccgac cgcgcccgcg atcaggacgg
      241 gaacgtattg catgaacccg gtcgcaccga ctccgccgtt ggttccggtc agttggtagg
      301 cgacataggg gagcccggcg ctttgcatcc aaccgccggt gttcgacacg aacgccgcga
      361 tccagaagat ccggaactcc cggtgttgca acgcaaccgt ggccccacga agcccaccag
      421 cactcatcgc tcgcgggatg cttcgacgcc agacattgca gccacgataa gcgtgccccg
      481 ataggctcaa cgccaatggc ccccgaacgc gacgccgcca tccgtcaagg cagtcaaggc
      541 cgtcaaggcg gaccaaggcg agaacccaaa ccgcgagacc ccatcctcac cagcgaatct
      601 ctcggccggg tcgctgcccg ggcgcagggg accgcctacg gatccgcgcc gaaggacggc
      661 tccaccgcat cgagttcgac cgcgctggta ccggtgaagc ccacgcggcg acgttcgcga
      721 cctgagttga ccgatgccga gatccgccgc ggcgacgtcg acgagtgggg ccgatccgaa
      781 cgagctcggc gcctcgcccg tttcctctac gaaccgctgt actcgtcata tttccgcgtc
      841 gaatgggaag ggctcgaaaa gatcccgatc gacggcgggg cgttgctcgt cgcgaaccac
      901 gcgggagcga ttccgtccga tgccccggcg atcatgcacg gcatcgagac gcagcttcgt
      961 cggccggtct acggcctcgc cgacaacttc tttcgcaccc tgcccgtcgt cggcaccatg
     1021 tggtcacgac tcggcggcct cccggctcac ccagacaacg cctaccgcct gctacgggag
     1081 cagaatcagc tggtcctggt ctttcctgaa ggctccaagg gtccggcgaa gcggatggac
     1141 gaacggtacc gactcgccag gttcggtcgg ggtgggttcg tggagatcgc catgcgagcg
     1201 ggcgttccca tcatcccgat cgcggtcgtc ggtgccgagg agtcgatgcc catcgtcgcc
     1261 aagttgccgt cgttggccgg agtgttcggt gctccgtatg tccccatcac cgccaacatg
     1321 ttgttgttcg gaccggcggg tctgttcacg tacttcccgt cgaagttcaa actccgggtg
     1381 ctcgacccga tccatttcga cgtcgaaccc gacctcgccc gctactcaaa gagccggatc
     1441 atggacgaat ccgaaaagat ccgcgatgag atccaggacg ctctgtacga aatgctgagc
     1501 aaacgacgat cggtctggtt cggctgatgg cccccggatc gacctacgaa tccgtcgaac
     1561 gttccggcga acggccagcg gttactcctc cccgaggccg ccgggtcctc gtcacgggcc
     1621 tcggttcctt ctggggtggt cgcatcgcgc aggcacttga ggacgacccc tgcgtcgaag
     1681 tgatcctcgg gctcgacacg accgaaccga ccgtcgaact cgaacgaacc gaatacgtgc
     1741 gcgtcgacgc caactactcg atcctgcaca agatcgtggc cgcggcgaag atcgacacga
     1801 tcatccatac gttcttgatc gtcgacccca ccatcatgac cgctcgggcg atgcacgaag
     1861 tcaacgtcat cggcacgatg aaccttttcg cagcggcgtc cgcaccggac tccacggtgc
     1921 ggaacgtgat cgtgaaatcc tccaccgcgg tctacggctg cgcggcgagc gacccgacgt
     1981 ggttcaccga ggacatggag ccgtccgcac cgctgcgacg cccggtcgaa cgcagcctcg
     2041 accaggtcga aagctacgtc cgcgacttcg cggaggacaa cccgcatgtt cgcgtctcac
     2101 tgcttcggtt ctcgaacgtc atcggcaccg agatctccac cccgctcacc cgagcgttgg
     2161 aactcccact cgtaccgtcg gtgctcggat tcgatccgcg gtttcagttc gtgcacgaaa
     2221 gcgacgtgat ccggtcgatc ctgtacgtgc tggaccacga tctgtccggc gtctacaacg
     2281 tcgccggcga cggtctgctg ccgtggagcg aggtggtctc gctgtgtggg aaacggcgcc
     2341 tgccgctcat ggcccgagtc acctccctcg ccacgttgcc gctgcgccag ttcgggctgt
     2401 ccggacttcc cgaggagtac ctcaacctgt tgcgctacgg acgcggcgtc gacaacacga
     2461 agctgaagca atccggcttc gaatacgact acacgtcggc ccaagcgatc aaggcgttca
     2521 tcgagggact tcgactccga cgcaccgtag gatcccactc cggcgagtac cgatacgaac
     2581 gcgacgtcga gcagttcttc cgacactccc ccgccgtcgt gcgcgaggcc ccaccctacg
     2641 atcggcgcaa gccctccgat gccaccgacg aggccatcga ggccaccgac gatcggatcg
     2701 actcccagcg gtaacgctcc tcgtatcgcg cctcggcggc atcgatcacc gatcggcggg
     2761 cgtcgatgtt gcgcacggcc tcgatgcacg cctcagcgat tccctcgtca tcgtcgcgaa
     2821 tccaggcgtg gacaccgtcg acgatgtcga tcccctcgca cccgatcgat gtcgtgacca
     2881 tcggtaggcg attcgccatc gcctccacaa ctttgagtcg agttcctgcg ccggcacgta
     2941 acgggacgac cgcgacgtcc gccccacgca gctccgcttc gagggaatcg accgccccca
     3001 cgacctccac cccatcgagg cgctcaagcg atgccaccga ctcggggttg cgcccaacga
     3061 tacgaaaccg cgcccccgga agctcgcgtc ggatgcgagg catcaccgcc gaggcgaaac
     3121 ggcggacagc atcagaattc gggtcgtagc tcatcaaccc gacgaacagg aacaccggtg
     3181 cagtcgagtc gttcaactcg acgtggtcgg caggttcaaa ggtccgctcg tagccgttcg
     3241 gcacgacgac ggtgttggga tatccgcttc gttccacatc gagtgagctg cacacaaacg
     3301 ccaggtcgac cgaattcgcc agacggcgtt gcaccgagtc ccagcgccgc tcatccacca
     3361 gattgaacgc actggccacc acccaccgag ccgacgctgc aacgcgtcgt cggaccgacg
     3421 ctcccggctc gatgatcggg cccgcctcgc gaatcgtgcg cgtcaggagg ttctcgagat
     3481 tgtcgaaatc gacgatcgtg gggatcgtga ggagatcctc gatcgggtac cagcagtcga
     3541 cgtggctgac gaagcagacg tcgtagttcg actgatcgag ggtttc
//
LOCUS       DAOOMA010000241         3585 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_31502_length_3585_cov_3.704249, whole genome shotgun sequence.
ACCESSION   DAOOMA010000241 DAOOMA010000000
VERSION     DAOOMA010000241.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 3585)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3585)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3585
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31502_length_3585_cov_3.704249"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..883
                     /locus_tag="PLP95_07380"
     CDS             <1..883
                     /locus_tag="PLP95_07380"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HPB45659.1"
                     /translation="VMTDASPTGSDPATNSGSPADRSWHEIAAQWPADNVAIAVVNSG
                     GIVESFGDLDRRFRLASVTKPLVATAIWLAVEEGALTPDTPAGPPGSTVRHLLCHASG
                     VGPDDDTILAAPGTRRIYSNHGFALLAQALEAHASMTVEHYLHDGVLEPLAMSATSFD
                     GPPGSGAVGSARDLATWVMSMLNPGTLLDSSTIAEATTVQFADIDGVLPGYGTQRPNP
                     WGLGVEIRGHKSPHWTGSLNSPETYGHFGQTGTFVWIDPVRSLGLVGLGDLDFGNWAL
                     PLWPVLSDSVIRTFGGT"
     gene            complement(779..1792)
                     /gene="lgt"
                     /locus_tag="PLP95_07385"
     CDS             complement(779..1792)
                     /gene="lgt"
                     /locus_tag="PLP95_07385"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: protein motif:HMM:NF013915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HPB45660.1"
                     /translation="MLSSIPSPSSNTMPVIGLHYYGVMIALGVLAAVMLARARWRAAG
                     HDPDEIADIAVWAVPAGLIGTRVYHVITDFDKYRDNLGQIFAIWNGGLGIPGGILFGV
                     IAGVWAARHYGIDVNRVADACIPGLPLAQAIGRLGNYFNQELFGRPTDVPWAIEIDPI
                     HRPAQYVQFETFHPTFAYEALWNLGLCAALILVGRRKVLRPGKLLPLYLIGYGVGRFW
                     VELLRSDHATEILGLRVNTWTSGAMVLIGFGWLLFGGALRPEAERGWDPEPEVAPEAE
                     GVAGAAEGEGAAGAAGEAGVEGEAGVGSGPAEGANDGVAEDGPQGQGPVSEVEVPEAD
                     KPE"
     gene            complement(1837..2556)
                     /locus_tag="PLP95_07390"
     CDS             complement(1837..2556)
                     /locus_tag="PLP95_07390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB45661.1"
                     /translation="MLAGVLAAGSMMSGCTASRPDPSGAEGPATTAVRYSRDVEGIVA
                     SLSDLLSNDGSDLSHWAPPEDQAKCGAERLVRQFTANGLLDRGFDPNDATLVLEFTSQ
                     DRTAAINVLIGCIDFETGYTSLLSSYEKMSVAEAACVGSGMRRLGIDRDIAGSLVDGA
                     APDPLANGQRFGQGMSELATQCLATSTLSDQPLSLPPPAPADAPRVERSEEVTSTTAA
                     SPGTGPDLRGIVPGGPLDTSN"
     gene            complement(2676..3152)
                     /locus_tag="PLP95_07395"
     CDS             complement(2676..3152)
                     /locus_tag="PLP95_07395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012230882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CarD family transcriptional regulator"
                     /protein_id="HPB45662.1"
                     /translation="MFDVGDRVVYPHHGAAIIVDRESREFQGETTDYLVLKMAHGDLT
                     LMVPVDKADEVGMRPPIGNEEVQDLFELLGKKDIREPANWSRRFKNHQEKLKSGDVYQ
                     VAEVVRNLALRDQSKGLSAGEKSMFVKARGVLVSELSFALDVTEQEALDRVQATLS"
BASE COUNT          636 a         1347 c         1044 g          558 t
ORIGIN      
        1 tgtcatgacc gatgcgtccc ccaccggctc cgaccccgcc accaactccg gctcccccgc
       61 cgatcgctcg tggcacgaga tcgccgcgca atggccggcc gacaacgtcg ccatcgccgt
      121 cgtcaactcc gggggaatcg tcgaatcgtt cggcgacctc gatcgtcgat tccgtctcgc
      181 ctcggtgacc aaacccctcg tcgccaccgc catctggctc gccgtcgagg aaggcgccct
      241 cacccccgac acgccggccg gtcccccggg ctccacggtg cgacacctcc tgtgtcatgc
      301 ctccggggtc gggcccgatg acgacaccat cctcgccgca cccggcaccc gccgcatcta
      361 ctccaatcac ggtttcgcac tactggcgca ggcgctcgaa gcccacgcta gcatgaccgt
      421 cgagcactac ctgcacgacg gcgtcctcga accgctcgcc atgtcggcca cctctttcga
      481 cggcccgccc ggatccgggg ccgtcggctc ggcccgcgac ctcgccacct gggtgatgtc
      541 gatgttgaac cccggcacgc tgctcgattc ctcgacgatc gccgaggcca ccaccgttca
      601 gttcgccgac atcgacgggg tactgcccgg ctacggcacc caacgaccca acccgtgggg
      661 gctcggcgtc gagatccgcg gccacaaatc gccgcactgg accggctccc tcaactcacc
      721 cgagacctat ggccacttcg gccagaccgg caccttcgtg tggatcgacc ccgttcgatc
      781 actcgggctt gtcggcctcg gggacctcga cttcggaaac tgggccctgc ccctgtggcc
      841 cgtcctcagc gactccgtca ttcgcacctt cggcgggacc tgacccgaca ccggcttcgc
      901 cttcgacgcc ggcctcgcca gcggcaccgg ccgcaccctc gccctcagcg gcaccagcca
      961 caccctcagc ctcgggcgcc acctcgggct cgggatccca gccgcgttcg gcctccgggc
     1021 gcaacgcccc accgaacagc aaccacccga agccgatcag caccatcgcc ccggaggtcc
     1081 aggtattcac ccgcaatcca agaatctcgg tcgcatggtc ggaccgcaac aactcgaccc
     1141 agaaccgacc cacgccatag ccgatcagat acagcggcaa cagcttgccg ggccgcaaaa
     1201 ccttgcgacg accgaccagg atcagcgccg cgcacaaccc gaggttccac aacgcctcgt
     1261 acgcgaacgt cgggtggaac gtctcgaact gcacatactg cgcaggccgg tggatcgggt
     1321 cgatctcgat cgcccacggc acgtcggtcg gacgcccgaa cagctcctgg ttgaagtaat
     1381 tgccgagccg gccgatcgcc tgcgccagcg gcagacccgg gatgcacgca tcggccaccc
     1441 ggttcacatc gatgccgtag tgacgagccg cccagacacc ggcgatcacc ccgaacaaga
     1501 tgccgccggg aatccccaac ccgccgttcc agatggcgaa aatctggccg aggttgtcgc
     1561 ggtacttatc gaaatcggtg atgacgtggt acacgcgggt gccgatcagc cccgcgggca
     1621 ccgcccacac cgcaatgtcg gcgatctcgt cggggtcatg gcccgccgca cgccaccgcg
     1681 cccgagccag catcaccgct gcgagcacac cgagggcgat catgaccccg tagtagtgaa
     1741 gcccgatcac cggcatcgtg ttcgaactcg gactgggaat cgacgacagc aacggtggtc
     1801 tcctcggtga gatgccgagc cagcttaggc ccgcgctcag ttcgatgtgt cgagggggcc
     1861 gccgggcacg atgcctcgca aatcggggcc ggttccgggc gacgccgcgg tggttgaggt
     1921 cacctcctcg gaccgctcga ccctcggagc atcggcgggt gccggcggcg ggagactcag
     1981 aggttggtcc gacagtgtgc tggtcgccag acattgggta gcgagctcgc tcatcccctg
     2041 cccgaaacgc tgaccgttcg ccaacggatc cggtgctgca ccgtcgacca acgatcctgc
     2101 gatgtcgcgg tcgattccga gtctgcgcat ccccgaaccg acacaggccg cctcggccac
     2161 cgacatcttc tcgtacgacg acaacaacga cgtgtacccg gtttcgaaat cgatacaacc
     2221 gatcaacacg ttgatcgcgg ccgtgcggtc ctgcgaagtg aactccaaaa ccagcgtcgc
     2281 gtcgttgggg tcgaagcccc ggtcgagcaa cccgttcgcg gtgaattggc gcaccaggcg
     2341 ctcggctccg cacttcgcct gatcctccgg cggcgcccaa tggctcaggt cggagccgtc
     2401 gttactcagt agatcgctca gcgacgccac gattccctcg acgtcgcggg agtaacgcac
     2461 ggcggtggtc gccgggccct ccgcgccgga ggggtccggg cgcgaagccg tgcacccact
     2521 catcatcgaa ccggccgcca acacccccgc caacacccat ccacgaccgc gccgacggcg
     2581 ttcatcgacg cggcggccgg ggcgcgatcc gtgatcggga cgggagccgc gatcgggacg
     2641 cggaccggag ccgacgcggg gacggcggct caggttcaag acagcgtggc ctgaacccgg
     2701 tcgagtgcct cttgttcggt gacgtcgagt gcgaagctca actccgacac cagcactcca
     2761 cgagccttca cgaacatcga cttctcgccg gccgacagcc ccttgctctg gtcgcgaagc
     2821 gcaaggttcc gcaccacctc ggcgacctga tagacgtcgc ccgacttcag cttctcctgg
     2881 tggttcttga agcggcggct ccagttggcc ggctcgcgga tgtccttttt acccagcagt
     2941 tcgaacagat cctggacctc ttcgttgccg atgggcggac gcatgccgac ttcgtccgcc
     3001 ttgtcgaccg gcaccatcag cgtcagatcg ccatgagcca tcttcaacac caggtaatcg
     3061 gtggtctctc cctggaactc tcgcgactca cggtcgacga tgattgcagc gccgtggtgg
     3121 ggataaacaa cgcgatctcc gacatcaaac acgacttact cctcgaagct ctcggggcca
     3181 catcggaccc agccgggccg agggtgccac acatcggacc acccagccaa tcggacgcac
     3241 caccgggtac gacccctcta ttgtgccgca tttttcgagc ggaatccgcc cagacggtgt
     3301 atcggtcgtc acggcttgcc cgcgtgccgt tggttcccgg gtcctcccag cctcgcccgc
     3361 gacacgcccg cgacacgccc gcgaccccac cgcgacctca aagaaccggc gacggacctt
     3421 tgacagatcg gatccgaggc ggtaactgaa tacctggagc cggtgcgagt ggacacagcg
     3481 cttcgccgat tcgagaggct catggaccat caccaacgac ccctgcgaca gcgactccgt
     3541 tccgccaccc ccgacgacac cgccgggatc gtggcactgt tgtcg
//
LOCUS       DAOOMA010000242         3576 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_31601_length_3576_cov_4.606930, whole genome shotgun sequence.
ACCESSION   DAOOMA010000242 DAOOMA010000000
VERSION     DAOOMA010000242.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 3576)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3576)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3576
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31601_length_3576_cov_4.606930"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1863)
                     /locus_tag="PLP95_07400"
     CDS             complement(<1..1863)
                     /locus_tag="PLP95_07400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB45663.1"
                     /translation="MVTLANRVEPVPVDGEADDDVGDPFELVAAATIRGQRPGRLVYP
                     LVELGGGADRRPVELDSSTLDRLHSATPTMARIAVNREQAAVVCDIADFYTEATTTIA
                     ATHMLQLHAEALRAAGATPWFDEAATASFAARVSPSDRSPRARSRARRLANDVIAAAG
                     IPSSARSPTGDYAIGWMSDGPGAFGSESGLALANGNNLAWVRLVESDGISFVRVHGTF
                     GTIPGAADSRPLRRHIDLLNSEVEVVRVATVPAPRGKRRYLIAVCDVPVSQFSPRMLE
                     HMVWAVATLLDWGVDDALDSGLVVPDGAGADPISVDFVRPERWLKHQVAPRLVKRSSN
                     ERTADHRLAIDDGGDVAAAPEAVPDAAPPRPRGTSLSPNSTTWREVAAPHLEMLIRRD
                     DVSLLKSNDDVTANDLSVNDLAAAALRLLIAGPAAFGRLTSLASQAGPEAIVGHVDEV
                     VISSAIAEQHRLRALSDAVGAWGRTQPRQVREQMSSALQPMLGTRHPGFEATIDTALG
                     LDDAAPSERLSTIATHLCSLDVDDASYPLQLVDLAIDSISSGTLMARTAYSASSDRAP
                     LAAVLATAAALHQCSLEPDVIRAIQYLAQPLRAADLLQLRSGDRIGPIVKRRGRH"
     gene            complement(2062..3420)
                     /locus_tag="PLP95_07405"
     CDS             complement(2062..3420)
                     /locus_tag="PLP95_07405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB45664.1"
                     /translation="MANSGIGALGHGERIDLARRATVAHRPDDSRVTTDAHRLVALAS
                     TVATPQTSSQTQALADELASLLAPQITRASDIRGNNRIWLMDLLHAYAQLTTTMLEAW
                     YRSESLRGTEGAARRWLKEHDHLAVGEHPVVADLLHRIDTGMFRATPSDEEFRALFGL
                     ETSNTVELFAGRRDFRGFLPLLSPDLPSEFERRGIQPTGVHWWHAEDDVAALIAVTAL
                     LASRGVTVAFPSLQHQGVARRASSIQGFSGFGVGAGFSPYTHAVADASNLFTSSNDGE
                     TDTLEIRMTDDVASDARWQELTITSDDAPQVPFRRITDDTECLDHAIIARVPVNAHGV
                     VRVHWVVAGFTAMATAESTAWLCREWPWVLDQYRRCGNTLDRHQMVVVLGKQAVLGKQ
                     AVDYNAPLRPDDSPLRWVPRSVIWGDRHELAPPPPSRFRTNGVPGTDAAQVTPSGVPL
                     AR"
BASE COUNT          591 a         1155 c         1240 g          590 t
ORIGIN      
        1 gtgtcggcct cgccgtttca cgatcggccc gatccggtcg ccgcttcgaa gttgcagcag
       61 gtccgctgcc cgcagcggct gagcgaggta ctgaattgca cggatcacgt cgggttcgag
      121 cgagcactgg tgcagcgccg cagcggtcgc cagcaccgct gccaggggtg cgcggtcgga
      181 gctcgcagag taggcagtgc gcgccatcag cgttccggat gagatcgagt cgatcgccag
      241 atcgaccagt tgaagcggat agctcgcgtc gtcgacgtcg agcgagcaaa gatgcgtagc
      301 gatcgtgctc aggcgctcgg acggtgccgc atcgtccagg ccgagcgcag tgtcgatcgt
      361 cgcttcgaac ccgggatggc gagttcccaa catcggctgc aacgccgagc tcatctgttc
      421 gcgtacctgg cgcggttggg ttcggcccca ggccccgacg gcgtcggaca acgctcggag
      481 ccgatgctgt tcggcgatcg ccgagctgat caccacctcg tcgacgtggc caacgatggc
      541 ctcgggtccc gcctgcgagg ccagcgacgt gagccgtccg aacgcggcgg gcccggcgat
      601 cagcagacgc aacgccgcgg ccgcgaggtc gttgacggag aggtcgttcg ctgtgacgtc
      661 gtcgttcgac ttgagcagcg aaacgtcgtc acggcgaatc agcatctcca ggtgaggtgc
      721 ggccacctcg cgccacgtcg tcgagttggg gctgagcgac gtgccgcgtg ggcgaggggg
      781 cgcggcgtcc gggacagcct ccggcgcggc ggcgacgtct ccgccgtcgt cgatcgcgag
      841 gcggtgatcg gcagttcgct cgttcgagga tcgcttcacc agacgcgggg cgacctggtg
      901 cttcagccac cgttcgggtc gcacgaaatc gaccgatatc ggatccgcgc cggctccgtc
      961 aggtacgaca agtccggagt cgagggcatc gtcgaccccc cagtcgagca gtgtcgctac
     1021 ggcccacacc atgtgttcga gcatgcgcgg ggagaattgc gacacgggca cgtcgcacac
     1081 ggcgatgaga taccggcgct taccccgtgg cgctggaacg gtagcgacac gcacgacctc
     1141 aacctcggaa ttcaacaggt cgatatggcg ccgcagcggc cgggaatcgg ccgctcctgg
     1201 gatcgtgccg aaggtgccgt ggacccgtac gaagctgatt ccatccgatt cgacgagtcg
     1261 cacccaagcg aggttgttcc cgttggcgag cgcgagtccc gattcggaac cgaaggcccc
     1321 cggaccgtcc gacatccagc cgatcgcgta gtcgccggtc ggcgatcggg ctgagctggg
     1381 aataccggct gcggcgatga cgtcgttggc caaccttcgg gcacgggacc gggcccgtgg
     1441 ggaacgatcg ctcggcgaga ccctcgccgc gaacgacgcc gtggcggctt catcgaacca
     1501 cggggtcgcc cccgcggccc gcagcgcctc ggcgtgcaac tgcaacatgt gggtcgcagc
     1561 gatcgtcgtc gtggcttcgg tatagaagtc ggcgatgtcg caaacgacgg ccgcctgttc
     1621 ccgattgacg gcgattcgcg ccatggtcgg cgtcgccgaa tggagtcgat cgagcgtgct
     1681 ggagtccaac tcgaccggtc ggcggtcagc gccaccaccc agttcgacga gcggatagac
     1741 caggcgcccg gggcgttggc ctcggatggt tgccgccgca acgagttcga aggggtcgcc
     1801 gacgtcgtcg tcggcctcgc catcgaccgg gacgggctcg acccgatttg cgagtgtcac
     1861 cactcgtcga acgaaagctc gaaccggtcg ttcgatcgtc gcgtccggcc gatcgccaaa
     1921 atgtgtcatc ggagtcctcc cagcggttct ggcacctgca ggtgcggact ccagcgtggg
     1981 cccgaccgac acgaccgaac cacgggatca actcagcagt ttgtcgctat cgccggtgtt
     2041 atcagcaccg agccgagaca tctatcgggc taacggaacg ccgctgggcg taacttgagc
     2101 cgcgtcggtc cctgggaccc cgttggttcg gaacctcgac ggtggcggcg gcgcgagttc
     2161 gtggcggtct ccccaaatca ccgaacgtgg cacccagcgc aacgggctgt cgtcgggtcg
     2221 gagcggagcg ttgtaatcga ccgcctgctt gcccagtacc gcctgtttgc ccaggaccac
     2281 caccatctga tgtcggtcga gcgtgttgcc gcaccggcgg tactgatcga gcacccatgg
     2341 ccattcacgg cagagccacg cggtcgactc ggcagtcgcc atcgccgtga aacccgcaac
     2401 cacccagtgc acccggacga cgccgtgagc gttcaccggt acgcgggcga tgatcgcgtg
     2461 gtcgaggcat tcggtgtcgt cggtgatgcg gcgaaacggc acctgcggcg cgtcgtcgga
     2521 cgtgatggtg agttcctgcc accgtgcgtc cgaggcgacg tcgtcggtca tgcggatttc
     2581 gagcgtgtcg gtctcgccgt cgttgctgga ggtaaagagg ttgctcgcgt ctgcgaccgc
     2641 atgggtatag ggcgagaacc cggcccccac gccgaacccg ctgaagccct gaatgctcga
     2701 ggctcgccga gccacgccct ggtgttgtag cgagggaaag gccacggtaa cgccgcggct
     2761 ggccaacaag gcggtgaccg cgatcagcgc ggccacgtcg tcttcggcat gccaccagtg
     2821 cacgccggtt ggttgaatcc cccgacgctc gaattccgac gggagatcag gggacaacag
     2881 cggcaggaag ccgcgaaaat cgcgtcgtcc ggcgaagagt tcgacggtgt tcgacgtttc
     2941 gagcccgaac agcgcccgaa actcctcatc gctcggggtt gcccggaaca tcccggtgtc
     3001 gatccggtgc aacagatccg cgacgaccgg gtgctcgccc acggcgaggt ggtcgtgctc
     3061 cttgagccac cgtcgggccg cgccctcggt gccgcgaagc gactcggatc gataccaggc
     3121 ctccagcatc gtggtggtca gttgggcgta cgcgtgcaag aggtccatca accagatgcg
     3181 gttgttgcct cggatgtcgg aagccctcgt gatctgcgga gcgaggaggc tggccaactc
     3241 gtccgccaat gcctgggttt ggctcgacgt ctgaggcgtt gcgacggtgg aggcgagggc
     3301 gacgagccgg tgagcgtcgg tggtgaccct cgaatcatcg ggacggtggg cgacggtggc
     3361 gcggcgggcg agatcgatcc gctcgccgtg gccgagggca ccgattcccg agttggccac
     3421 gatccgccgc cagtcctcac gacgaggggt tgcctcggta agcgatcgtt tgcccgccag
     3481 ctccattgtc gcgttcgggc agcgcgtgag gcgctgaaac gctagggcgg agtcaccagg
     3541 attcccggcg ccgggaatcc tggtgactcc gcccta
//
LOCUS       DAOOMA010000243         3567 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_31699_length_3567_cov_3.459567, whole genome shotgun sequence.
ACCESSION   DAOOMA010000243 DAOOMA010000000
VERSION     DAOOMA010000243.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 3567)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3567)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3567
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31699_length_3567_cov_3.459567"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            478..783
                     /locus_tag="PLP95_07410"
     CDS             478..783
                     /locus_tag="PLP95_07410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB45665.1"
                     /translation="MLYDGFTFNAISWLEGLGFCGYGEAQDWLDGGRRIALDGDLPLN
                     PHGGQLSEGRTHGFGFIHEAVTQLRGDAGDRQVADARVAVVSTGGGTPSGAFLLRRD"
     gene            complement(880..1785)
                     /locus_tag="PLP95_07415"
     CDS             complement(880..1785)
                     /locus_tag="PLP95_07415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015357342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
                     /protein_id="HPB45666.1"
                     /translation="MSEYQFITYETLDDGRIARIMLNRPEARNAQNRGMLVELDEAFL
                     RAEADDEVRVVIFGGMGPMFTAGHDLGSKVSQEEYATHPSWKVNGGTRQGAERLMLQE
                     WHHYFNNTRRWRNLRKITVAQVHGPVFAAGLMLMWACDLIVAADDTVFADVVGTRLGM
                     CGVEYFAHPWELGPRRAKELLLTGDSLSVEEAVQIGMVSKVFPLDELSERTVEFARRI
                     AEQPTMSALLIKEAVNQSVDNMGFYNALNACFTLHELNHSHWAQVHENGFPVALPEDG
                     IQDWRTAPPVQTRDPYSVGGEVSPQ"
     gene            complement(1838..2167)
                     /locus_tag="PLP95_07420"
     CDS             complement(1838..2167)
                     /locus_tag="PLP95_07420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB45667.1"
                     /translation="MTRGLLIVESAPSSPDRVEEYHRWYDEVHIPQLLSFDLFVSARR
                     FEPVGGVGAGVGAAEGPFVAVYELEADDIAAAFAKYRAASARGELDRSDALQTDPPPR
                     VRLLIER"
     gene            complement(2164..3000)
                     /locus_tag="PLP95_07425"
     CDS             complement(2164..3000)
                     /locus_tag="PLP95_07425"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HPB45668.1"
                     /translation="MSTFQAFRFDGKRVLVVGGATGMGAAAAQLAVDAGAEVIVADFA
                     EITQSGVQTVHVNLAETASIDALVGELGAPIDALLSCAGVADGTPNIEKVNFLGHRYL
                     IDKLVDGGLLGRGSSIGFISSAAGLGWEANLELLNGLLDTPDFEGGAEWFAQNGKADY
                     YHTKQAVCAYVAREAFTFMKQGIRINAICPGPTETPLAKANADTWLAFGEDYRQELGI
                     EASTPLEQAYPLLFLVSDAAVAISGITLVTDVGYMSSGLTESFPPATFLAKLLNGRPV
                     EM"
     gene            complement(3073..>3567)
                     /locus_tag="PLP95_07430"
     CDS             complement(3073..>3567)
                     /locus_tag="PLP95_07430"
                     /inference="COORDINATES: protein motif:HMM:NF014564.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HPB45669.1"
                     /translation="LSRNYDTGDGRTLAFTCLQGAKYWPTLCVAIGREDLANDERFAD
                     QPSLLANSQAAAALLEEVFATATLEEWRQRLESFDGQWAVVQNTLEAAVDPQTLANGY
                     LQDLTTSGGVAFQLVSAPVQFDETPAPSCRAPEFNEHGDEILEGIGLDWDRIVDLKVK
                     GVVA"
BASE COUNT          602 a         1168 c         1175 g          622 t
ORIGIN      
        1 gggcatggga cgcgtcgagg gtccgaacat ggagtggcgt gcgccgttcg gagcgatgtc
       61 ggccgcgaac tggatcggcc tcaacgcctc gcaatacctg caccgctacg gcgccacccg
      121 cgaaatgctc ggctggatcg ctctcaacgc acgcgcgaac gctgccctca accccgacgc
      181 gatctatcgc gacccgatga cgatggacga ctatctgtcg gcccgcatga tcaccacccc
      241 gttcggtctg tacgactgcg acgttccgtg tgatggagcg atcgcggtgg tcgtgtcggc
      301 cgccgacacg gtcgacgacc tcgcgcagcc ggcgattcgg ctcgatgccg tcggcaccca
      361 gatcaccgaa cgggtgtcgt gggatcaggg caccctcacc catgaaccgc ggtcctcggc
      421 caggcggctc acctgtggac ccgtaccgac ctcacaccgg gcgatgtcga cgtcgccttg
      481 ctctacgacg ggttcacctt caacgccatc tcctggcttg aagggctcgg attctgcgga
      541 tacggcgagg cgcaggactg gctcgacggg gggcgtcgca tcgccctcga cggcgacctt
      601 ccgctcaacc cgcacggcgg ccaactctcc gagggccgca cccacggatt cggtttcatc
      661 cacgaggccg tcacccagct tcgcggcgac gccggcgatc gacaggttgc agacgcccgc
      721 gtcgcggtcg tgtcgaccgg cggcggcacc ccgtcgggtg ccttcctgtt acgccgagac
      781 tgaccgcctc acccgcgccg ccctcgccgt tctgtaggta ctttcggtgg tcatagccac
      841 cgaaagtacc tacagaacgg actattgccc gggaacggac tattgcgggg aaacctcgcc
      901 gccgaccgaa tacgggtcgc gggtctgcac cggcggtgcg gttcgccagt cctgaatacc
      961 gtcctcgggc aaggcgaccg ggaacccgtt ctcatgcacc tgcgcccagt gcgagtggtt
     1021 gagttcgtgc aaggtgaaac aggcgttgag cgcgttgtag aagcccatgt tgtccacgct
     1081 ttggttcacc gcctccttga tgagcagcgc cgacatcgtc ggttgttcgg cgatacgacg
     1141 ggcgaactcg acggtgcgct cggagagttc gtcgagcggg aacaccttcg acaccatgcc
     1201 gatctgcacg gcctcctcga ccgacaacga gtcgccggtc aacaacagtt ccttggcccg
     1261 gcgcggcccg agttcccacg ggtgggcgaa gtactcgacc ccacacatgc caaggcgcgt
     1321 accgacgaca tcggcgaaca cggtgtcgtc ggcggcgacg atcaggtcac acgcccacat
     1381 caacatgaga ccggcggcga acaccggccc gtgcacctga gcgacggtaa tcttgcgcag
     1441 gttgcgccac cgccgggtgt tgttgaagta gtgatgccac tcctgcagca tcagccgttc
     1501 ggcgccctgc cgggtaccgc cgttgacctt ccacgacgga tgggtggcgt actcctcctg
     1561 ggagaccttc gagccgaggt cgtggcccgc ggtgaacatc ggccccatcc ccccgaagat
     1621 cacgactcga acctcgtcgt cggcctctgc ccgaaggaac gcctcatcga gctcgacgag
     1681 catcccccgg ttctgggcat tgcgggcttc gggacggttc aacatgatgc gagcgatccg
     1741 cccgtcgtcg agtgtctcgt aggtgatgaa ctgatactcg ctcatggctc tcctcggtgg
     1801 ggcaacgctt cagccgtggg ctgcgtgggc ggcatcatca tcgttcgatg aggaggcgca
     1861 cccgtggcgg cgggtcggtt tgcaatgcat cgctgcggtc gagttcaccg cgggccgagg
     1921 ccgcccggta cttcgcgaag gccgcggcga tgtcgtcggc ttcgagttcg tacacggcga
     1981 caaaggggcc ctccgcggct cccaccccgg ctcccacccc accgaccggt tcgaatcggc
     2041 gcgccgagac gaacaggtcg aaggagagga gctgaggaat gtggacctcg tcgtaccagc
     2101 ggtgatactc ctcgacccga tcgggagacg acggcgccga ctcgacgatc aacagccccc
     2161 tggtcacatc tcgaccgggc ggccgttcag caatttggcg aggaaggtcg ccggcgggaa
     2221 cgactcggtg agtcccgaac tcatgtaccc gacatcggtg accagcgtga tgccggagat
     2281 ggccacggcc gcgtcgctga cgaggaacaa cagcggatag gcctgctcaa gcggtgtcga
     2341 ggcctcgatg ccgagttctt ggcggtagtc ctcgccgaag gccaaccagg tgtcggcgtt
     2401 cgccttggcg aggggggttt cggttggccc gggacagatg gcgttgatcc ggatgccctg
     2461 cttcatgaag gtgaacgcct cgcgggccac gtacgcacac accgcctgct tggtgtggta
     2521 gtagtcggct tttccgttct gggcgaacca ctccgcgccg ccctcgaagt ccggcgtgtc
     2581 gaggagaccg ttgagcaact ccagattcgc ttcccagccg aggccggccg cggaggagat
     2641 gaacccgatc gatgacccgc gaccgagcag gccgccgtcg accaacttgt cgatgaggta
     2701 gcggtgcccg aggaagttca ccttctcgat gttgggggtg ccatccgcga cgcccgcgca
     2761 cgacaacagg gcatcgatcg gagcgccgag ttcgccgacg agcgcgtcga tcgacgcggt
     2821 ctcggccagg ttcacgtgaa cggtttgcac gccgctctgg gtgatctcgg cgaagtcggc
     2881 gacgatcacc tcggctccgg catcgaccgc gagttgcgcc gcggccgcgc ccataccggt
     2941 ggcgccgccc acgacgagca cgcgcttacc atcgaatcgg aatgcttgaa aggtcgacat
     3001 gttctgcctc gctggtagtt gggaactagg gattggtggc tgttgagatg attcgaggtc
     3061 ggtggatgac gctcaggcga cgacgccttt gaccttcaga tcgacgatgc ggtcccagtc
     3121 gagaccgatg ccttcgagaa tctcgtcgcc gtgttcgttg aattcggggg cgcggcacga
     3181 aggggccggg gtctcgtcga actggaccgg ggccgacacg agctgaaacg ccacaccacc
     3241 agaggtcgtg aggtcctgca ggtagccgtt ggcgagagtc tgaggatcga ccgccgcttc
     3301 gagggtgttt tgcaccaccg cccactgtcc gtcgaagctc tcaaggcgct ggcgccactc
     3361 ctcgagcgtg gcggtggcga agacctcttc gagcagtgct gcggcggcct gactgttggc
     3421 gagcagcgat ggctggtcgg cgaatcgctc gtcgttggcg aggtcctcgc ggccgattgc
     3481 cacgcacagg gtcggccagt acttggcccc ctgcaggcag gtgaacgcca gcgtgcgtcc
     3541 gtcgccggtg tcgtagttgc gcgacag
//
LOCUS       DAOOMA010000244         3563 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_31739_length_3563_cov_3.500570, whole genome shotgun sequence.
ACCESSION   DAOOMA010000244 DAOOMA010000000
VERSION     DAOOMA010000244.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 3563)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3563)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3563
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31739_length_3563_cov_3.500570"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..70)
                     /gene="rpmF"
                     /locus_tag="PLP95_07435"
     CDS             complement(<1..70)
                     /gene="rpmF"
                     /locus_tag="PLP95_07435"
                     /inference="COORDINATES: protein motif:HMM:NF013908.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HPB45670.1"
                     /translation="MAVPKKKMSKAKTRMRRASAWKL"
     gene            complement(262..774)
                     /locus_tag="PLP95_07440"
     CDS             complement(262..774)
                     /locus_tag="PLP95_07440"
                     /inference="COORDINATES: protein motif:HMM:NF014657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceD family protein"
                     /protein_id="HPB45671.1"
                     /translation="MVAGNPLLINVVDVRRVPGSTRDVTAEVELAGLELSSARVAEDS
                     PLSIDLTLESVSGGVTAAGTITTTWQGECRRCLGLVVGELVLDCEEVFEDRPVEGETY
                     PIEHDQIDLEPMVREQVLLSLSQSTTGSATCEGPIPEELPVLVADDDEGSVSPKDPRW
                     AALDDLRLDR"
     gene            complement(831..1424)
                     /locus_tag="PLP95_07445"
     CDS             complement(831..1424)
                     /locus_tag="PLP95_07445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB45672.1"
                     /translation="MTKPIEEELDELTAEGNDAFRTEGLIRQLIEMVEVARAMPMSSS
                     VMVHRDEMLDILDDLLAGLPDELRAASWLLKEREEVLERANRDRANLINDAKTRVAHM
                     VEQREITKQAHLRAERIRDEADAEARRMRREAEDYCDQKLASFEVVLDRLSKQVAAGR
                     RKLAVTISESEAPRPQVGIYTDDADSDAAAFFDQEVD"
     gene            complement(1417..1899)
                     /gene="coaD"
                     /locus_tag="PLP95_07450"
     CDS             complement(1417..1899)
                     /gene="coaD"
                     /locus_tag="PLP95_07450"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012227523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HPB45673.1"
                     /translation="MTVVLYPGSFDPVHNGHVEIVETAARLFDRVIVAAVRNPQKGAP
                     LFTLEERQAMIEESFAHLPNVEVTLFSSLVVDLAKQKGADFIVKGLRAVSDFESELQM
                     AQMNRRISGVDTLFIPTTSSHSFLASKLIREIARFGGDVTSMVPEPVNRRLSGKYVND
                     "
     gene            complement(1970..2521)
                     /gene="rsmD"
                     /locus_tag="PLP95_07455"
     CDS             complement(1970..2521)
                     /gene="rsmD"
                     /locus_tag="PLP95_07455"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: protein motif:HMM:NF015559.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HPB45674.1"
                     /translation="MRVVAGTARGRSLQTPPGRTTRPTSDRVREAVFNALNSLDALAD
                     ATVLDGFAGSGAMGIEALSRGARHVTFVEADRRAVATIHANLAATGFTDRATVVPQRL
                     ESFLARPQPALPWDLVVLDPPYVFDAWDDLLATIRGRVVVIESDRPVSVPKPWDVVRD
                     RSYGTTVVRIAEWRSTPSAQAPS"
     gene            complement(2583..>3563)
                     /locus_tag="PLP95_07460"
     CDS             complement(2583..>3563)
                     /locus_tag="PLP95_07460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020378158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HPB45675.1"
                     /translation="VGTHALIQEAVTFRSLGAVVIDEQHRFGVEQRAALREANADGSA
                     PDVLVMTATPIPRTAAMTVYGDLDVSVLDELPPGRTPIATTWARTDPDVDAAWDLVRR
                     EVEAGRQAYVVCPLIEESEKLEVSSATETFERLGAVDLAGLRLGLLHGRMPAEDKEAT
                     MSAFRRGDLDVLVATTVIEVGVDVPNATVMVILDADRFGIAQLHQLRGRVGRGAAASH
                     CVLVAAEPSPDGIERLEAMVRTTDGFELAEVDLDLRGEGTIMGERQKGRNDLRLASLR
                     RDREWVGIAREVAFELVDSPDPAAAIALEALVGELDLFLEEDDEAFLLKG"
BASE COUNT          607 a         1235 c         1126 g          595 t
ORIGIN      
        1 acagcttcca ggcggacgca cggcgcatgc gggtcttcgc cttcgacatc ttcttctttg
       61 ggacggccat cgtcgttcct gtgcaatctg gggagggact tgtcaaccgc gcgcaccacc
      121 cgcatccgcg gcaggtgcac acaggggcca aagcatagtc caccgtcagt gccgtgtccg
      181 aatcctcacc gttccggcgc tgtcaccggt ccggcgctgt caccgtgacc agaggtcgcc
      241 gcggggttgg tcgagcgcgc cctaccggtc gaggcggagg tcgtcgaggg ccgcccagcg
      301 cgggtctttg ggcgaaacgg atccctcgtc gtcatcggcg accagaaccg gcaattcctc
      361 gggtatcgga ccctcacagg tcgcagaacc cgttgtgctc tgcgacagcg acagcagcac
      421 ctgttcacga accatcggtt cgaggtcgat ctggtcgtgt tcgatcgggt aggtctcccc
      481 ctcgaccggg cggtcctcga agacctcctc gcaatcgagc acaagctcac cgaccaccaa
      541 gccgaggcag cgtcgacact ccccctgcca ggttgtcgtg atcgtgccgg cagcggtcac
      601 cccgcccgac accgactcca gcgtcagatc gatcgacagc ggcgagtcct cggcgacgcg
      661 agcgctcgac aattccaacc cggcgagttc gacctcggcg gtgacgtcgc gggtcgaacc
      721 cggcacgcgc cgaacgtcca ccacgttgat cagcaacggg ttcccggcaa ccatgtcagc
      781 gacctaccgt catgtcagcg acctaccgtg acctaccgct cgtcgccgac tcagtcgact
      841 tcctggtcga agaacgcggc cgcatcggaa tcggcgtcgt cagtgtagat gccgacctga
      901 ggacgcggcg cctccgactc cgagatcgtc acagcgagct tgcgccgtcc cgccgccacc
      961 tgtttggaca gccggtcgag caccacctcg aagctcgcga gcttctggtc gcagtagtcc
     1021 tccgcttcgc gccgcatccg gcgcgcctcg gcgtcggcct cgtcgcgaat ccgctcggcg
     1081 cgcagatgcg cctgtttggt gatctcgcgc tgttcgacca tgtgggccac ccgcgtcttc
     1141 gcgtcgttga tgaggttggc acggtcgcga ttggcacgct cgagcacctc ctcgcgctct
     1201 ttcagcagcc aactcgccgc gcgcaactca tcgggcagtc cggcgagcag gtcgtcgagg
     1261 atgtcgagca tctcgtcacg gtgcaccatc accgacgatg acatcggcat cgcgcgtgcg
     1321 acctccacca tctcgatgag ttggcggatc aggccctcgg tccgaaaggc gtcgttgccc
     1381 tccgccgtga gttcgtcgag ttcttcttcg atcggcttag tcattgacgt actttccact
     1441 caaccgccga ttgaccggct cgggaaccat cgaggtcaca tcgccgccga accgggcgat
     1501 ctcacggatg agtttcgatg ccaggaacga atgcgacgag gtggtcggga tgaacagggt
     1561 atcgacgccg gagatccggc gattcatctg ggccatctgc agttcggatt cgaaatccga
     1621 caccgcccgc agacccttca cgatgaagtc ggctcccttt tgcttggcca ggtcgaccac
     1681 cagggaactg aagagggtga cttcgacatt cggcaggtga gcgaaggatt cctcgatcat
     1741 cgcctgacgt tcctcgagcg tgaacagcgg agcgcccttt tgtgggttgc gcaccgcggc
     1801 gacgatcaca cggtcgaaca gtcgcgctgc ggtctccacg atctcgacgt gtccgttgtg
     1861 cacgggatcg aacgaccccg ggtagagaac gacggtcaca ggggttgctc ctcgaattgg
     1921 tcttgcacgg attgaagggt cacgggctga acgggtgcgg gatgaacggt cacgacgggg
     1981 cttgcgctga tggggtgctt cgccactcgg cgatcctcac caccgtagtt ccgtagctgc
     2041 gatccctcac gacatcccaa ggcttcggaa cactaacggg tcgatcggat tcgatcacga
     2101 ccacgcgacc gcgaatcgtc gccagcaggt cgtcccaggc gtcgaacaca tacggcggat
     2161 ccaacacgac gaggtcccag ggcagcgctg gctgcggacg ggccagaaac gactcgaggc
     2221 gttgcggcac caccgtggcc cgatcggtga atccggtggc ggcgaggttg gcatggatgg
     2281 tggcgacggc tcgacgatcg gcctcgacga aggtgacgtg acgtgcgccg cgggacaacg
     2341 cctcgatccc catcgccccc gacccggcga acccgtccag caccgtggca tccgcgaggg
     2401 catcgaggct gttgagggcg ttgaacaccg cctcgcggac ccgatccgag gtcggtcgag
     2461 tagtgcggcc gggcggggtt tgcaggctgc gcccgcgcgc tgttccggcg accactctca
     2521 tcgggcgccg acgttagccg cccacacccc gccaggtggt cggggtcggg gccgggcggc
     2581 ggtcagccct tgagcaggaa cgcctcgtcg tcctcctcca gaaacaggtc gagttcgccc
     2641 accaacgctt cgagcgcaat cgccgcggcc ggatctggcg agtcgaccag ttcgaacgcg
     2701 acctcccggg cgatccccac ccactcccgg tcgcggcgaa gcgacgcgag ccgcagatcg
     2761 ttacgaccct tttggcgctc ccccatgatc gtgccctccc cgcgcaggtc gaggtcgacc
     2821 tcggccaact cgaatccgtc ggtggtgcga accatcgcct cgagccgctc gatgccatca
     2881 gggctcggct ccgccgccac gagcacgcag tggctcgccg ccgcgccacg ccccacgcgg
     2941 ccacgaagct ggtgaagctg ggcgataccg aaacggtcgg catcgaggat gaccatgacc
     3001 gtcgcattgg gaacgtcgac gccgacctcg atcaccgtcg tggccaccag gacatcgagg
     3061 tctccgcgac gaaacgccga catcgtggcc tccttgtcct cggcgggcat gcggccgtgc
     3121 aacaggccga ggcgaagacc ggcgaggtcg accgcgccga gccgctcgaa ggtctcggtc
     3181 gccgacgaca cctcgagctt ctcggactcc tcgatcagcg ggcacaccac ataggcctga
     3241 cgtccggcct cgacctcccg acgcaccagg tcccaggccg catcgacgtc gggatcggtt
     3301 cgcgcccagg tcgtggcgat cggggtacgc cccggcggga gttcgtcgag cacgctcacg
     3361 tcgaggtcgc catagaccgt catcgccgcg gtgcgcggga tcggcgtcgc ggtcatcacc
     3421 agaacgtcgg gggcggaacc gtctgcgttc gcctcacgca acgcggcacg ctgttccacc
     3481 ccgaaacgat gctgctcgtc gatcaccacc gcgccgagcg accggaacgt gaccgcctcc
     3541 tggatgagcg catgagtgcc gac
//
LOCUS       DAOOMA010000245         3523 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_32231_length_3523_cov_4.016436, whole genome shotgun sequence.
ACCESSION   DAOOMA010000245 DAOOMA010000000
VERSION     DAOOMA010000245.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 3523)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3523)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3523
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32231_length_3523_cov_4.016436"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            76..1035
                     /locus_tag="PLP95_07465"
     CDS             76..1035
                     /locus_tag="PLP95_07465"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HPB45676.1"
                     /translation="MPHALLSYLDDAERADYLATLTAVTYPPGAVIFTEGGPGERFMF
                     VTGGNASVTVGGEDVASVGAGSILGELALLRGTRRTATCVATEEVTGYQGTLEHLLDL
                     FDRSPDAGRDLAELTARRIAPRVPAVRVTLPDGGALRLRPLLATDHDAFMHVYESWPP
                     RKRYMRFFSAAPPDHVLRQLIDIDFVDHFAWIAFADNGDGIDEPTEAVGSARYIRVDD
                     PASAQIAFGVAEDWQRRGVASVLIAALGAAAQVNGITEFTAEVLSENLASQALLRKFA
                     MVFDFSEAGELHGTGPVADPSIALSDDEAEAMAQAAALITQVQ"
     gene            1069..2040
                     /locus_tag="PLP95_07470"
     CDS             1069..2040
                     /locus_tag="PLP95_07470"
                     /inference="COORDINATES: protein motif:HMM:NF019474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HPB45677.1"
                     /translation="MTLDSRVQKLLKFAALRPNDLTQPMSVRRTQSNAVSAKGAKLVM
                     RMGPNPAREVEYSVPVDGGRIRVRCLVPHGPGPHPLYVFIHGGGWCAGTIEEREGRCR
                     MVSDGARCVVASVDYRLAPENQFPTAPLDCFAALNFLVDHAEELNLDVTRIAVGGESA
                     GGNLAAALCLLARDRGGPEIMYQWLDVPALDCTLSQEGHRNVPDGYLLDAAAIDDYLE
                     SYIDDPAEYTNPLASPLLAEDLSGLPPAWIMTVGYDRLRGDGAAYADALIAAGVDARH
                     TRLDGHIHVSFAFTRLVPSAAAYEDRAIVALADAFRLAKVRVGSSAS"
     gene            2078..3523
                     /locus_tag="PLP95_07475"
                     /pseudo
     CDS             2078..3523
                     /locus_tag="PLP95_07475"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfatase"
BASE COUNT          567 a         1248 c         1125 g          583 t
ORIGIN      
        1 gtagctgtcc gacacggtgc tgtccgacac ggtgctgtcc gacacggcgc tgtcgcctcg
       61 cgtagccttc gcctcgtgcc acacgcgctg ctcagttacc tcgacgacgc cgaacgagcc
      121 gactacctcg ccaccctcac ggcggtgacg tacccgccgg gcgcggtgat cttcaccgag
      181 ggcggcccgg gggaacggtt catgttcgtc accggcggta atgcatcggt caccgtcgga
      241 ggcgaggacg tcgcttcggt gggtgccggt tccatcctcg gcgagttggc gttgcttcgc
      301 ggcacccgac gcacggcaac ctgcgtggcc accgaggagg tcaccggcta tcagggaacc
      361 ctcgaacacc tcctcgacct gttcgaccgc tcccccgacg ccgggcgcga cctggccgaa
      421 ctcaccgctc gacgcatcgc ccctcgcgtg ccggcggtgc gggtgaccct tcccgatggc
      481 ggggcgctgc gactgcggcc gttgctcgcc accgaccacg acgccttcat gcacgtctat
      541 gagagctggc cgccccgcaa gcgctacatg cggttcttct cggccgcccc gcccgatcac
      601 gtcctgcgcc aactcatcga catcgatttc gtcgatcatt tcgcctggat cgccttcgcc
      661 gacaacggtg acgggatcga cgagccaacc gaggccgtcg gttcggcgcg ctacatccgc
      721 gtcgacgatc cggcctcggc acagatcgcc ttcggcgtgg ccgaagactg gcaacggcgc
      781 ggcgtcgcgt cggtcctcat cgccgcgctt ggcgctgcag cgcaggtgaa cggcatcacc
      841 gaattcaccg cagaggtgtt gtcggagaac ctcgcgtccc aggcactgtt gcgaaagttc
      901 gccatggtct tcgatttcag cgaggccggc gagttgcacg gcaccgggcc ggtagccgac
      961 ccgtcgatcg cgttgagcga tgacgaggcc gaggcgatgg cccaggctgc tgcgctgatc
     1021 acccaggtgc agtaaccacg acgcggcggc cgtgtcagga tggctcatat gactctcgac
     1081 tcccgcgttc agaagcttct caagttcgca gcgctacggc ccaacgacct gacccagccg
     1141 atgtcggttc gtcgcacgca gagcaacgca gtctcggcca agggcgccaa gctggtcatg
     1201 aggatggggc cgaaccccgc ccgggaggtc gagtactccg tgccggtcga cggcggccgg
     1261 atccgggtcc ggtgcctggt tccgcacggg ccggggccgc atccgctcta tgtgttcatc
     1321 cacggcggcg ggtggtgcgc cggaacgatc gaggagcgcg agggacgctg ccggatggtc
     1381 tccgacggcg cccgctgtgt cgtcgcctcg gtcgattacc gactcgcccc cgagaatcag
     1441 ttcccgaccg ctccactcga ctgcttcgcc gcattgaact tcctggtcga tcacgctgag
     1501 gaactcaacc tcgacgtcac gaggatcgcg gtcggcggag agtccgccgg cggcaatctt
     1561 gcggcggcgt tgtgcctgtt ggcgagggat cgcggcggcc ccgagatcat gtaccagtgg
     1621 ctcgacgtcc cggccctcga ctgcacgttg tctcaggagg gccaccgcaa cgtgcccgac
     1681 ggctacctcc tcgacgccgc ggcgatcgac gactacctgg agtcctacat cgacgacccg
     1741 gccgagtaca ccaacccgct cgcgtcgccg ctgctcgccg aggatctcag cggtctaccg
     1801 cccgcgtgga tcatgaccgt cggctacgac cgccttcgcg gcgacggtgc ggcctatgcc
     1861 gacgcgctca tcgccgcggg cgtcgacgcg cgccacaccc gactcgacgg ccacatccac
     1921 gtctcgttcg ccttcacccg attggtcccg agcgcggccg cctacgagga ccgtgcgatc
     1981 gtcgcgctcg cggatgcgtt tcgactggcc aaggtccgcg tcggttcatc tgcgtcctga
     2041 gcgacgctcg ttcaaacaaa ccaaggagtc ggttgccatg acagagaatt tccaagggac
     2101 gatcggcaac acgctgtcgg agtcgacccc gtggtggccc gaggccgacc acggaccgac
     2161 cggggcgccg aacgtgatcg tggtggtgct cgacgatgtc ggctacgccc agttcggctg
     2221 ctacggctcc gatattgcaa ccccgacctt cgaccggctc gccgccggtg gtctgcgcta
     2281 ttcgaacttc cacaccacag ccctgtgctc ccccacccgc gcggcgctgt tgagcggccg
     2341 caatcaccac agcaactcga tggcgagaat catcgagttg gcctcgggat ttcccggtta
     2401 caactcccac atcccgcacg agaacggctt cctgtcggag atcctgcgcg atgccggcta
     2461 cgcgaccttc gcactgggca agtggcatct cgccccggcg acccagaccg ctgcgggcag
     2521 cccgaaggac aagtggccgc tcgggcgtgg cttcgaacgg ttctacggat tcctcggcgg
     2581 cgaaaccgat cagttcgctc ccgacctggt gcgcgacaac tcccacgtcc cgccgccacg
     2641 aacgcccgag gagggctacc acctcaccga ggacatcacc gacgaggcca tcggctacct
     2701 cgccgacctg cgaggttcga tgcagcggcg gccgttcttc atgtggttcg cccccggcgc
     2761 ttgccacgca ccccatcagg cgccggactc ctacatcgac gcctaccggg ggcacttcga
     2821 tcaggggtgg gatctgtggc gcgaacaggt tttcgcacgg caactcgcga gcgggttggt
     2881 gcccgaggga acccagttgt cgtcgcggcc ggcctgggtg ccggcgtggg actcgttgag
     2941 cgccgacgaa cagcgcctcg ccgcacgaat gatggaggta tacgccggat ttctcaccca
     3001 caccgacgcc taggtcggtc ggctactcga ctatgtcgaa cgtctgggcg aggccgacaa
     3061 caccatcgtg atcgtgttgt cggacaacgg tgcgtccgcc gagggcgggg cggccggttc
     3121 gttcaacgag ctgtacttct tcaacttcgt ccccgagcac ctcaacgaga acctgcgccg
     3181 tatcgacgaa ctcggaggac cgagggccaa caaccactac ccgtgggggt gggcgtgggc
     3241 aggaaacaca ccgctcaaac gcttcaagcg cgacacccac gagggcggcg tcaccgaccc
     3301 cctcatcatc tcgtggcccg ctgccatcgc gtcgccgggc acgacgccgc gccgctacgt
     3361 gcatgccatc gacctgatgc cgaccctgct ggagctcatc ggcatcgagt ccccacgatc
     3421 gatcgccggg gtccaacaga gcgagatcga agggacgagc ttcgctgaca cgctgctcgc
     3481 tggcggcggg gctggcggcg gggctggtgg cggcgaggct ggc
//
LOCUS       DAOOMA010000246         3515 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_32314_length_3515_cov_4.964451, whole genome shotgun sequence.
ACCESSION   DAOOMA010000246 DAOOMA010000000
VERSION     DAOOMA010000246.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 3515)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3515)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3515
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32314_length_3515_cov_4.964451"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            96..1187
                     /locus_tag="PLP95_07480"
     CDS             96..1187
                     /locus_tag="PLP95_07480"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HPB45678.1"
                     /translation="MSVSDDGLFLERGDDGWRLGGGDAGRFRLVNEYLSYLADRNYSP
                     RTTRTYGFGLLAFCRWLDSEDLDLASVTTDVLLQFLAACRTELVTGRLGGANVVGLDG
                     RRTDVLSPATVNMRLAAISGLYSFRSMRDPDISNPVPKGREAKRLSAGERTGMLAHVS
                     RAPKRRSPLRVRDTKRLPKTLDHREVIQLLESLRTWRDRAIAGLMVFCGLRSAEVLAL
                     DVGDVDIGARWLRVSGKGGKERRVPLDVDVAGVIQTYLLAERPDTASSRLFIVAKGPT
                     RGQPLSPAGLRRIFRYHRDLTGITAAHPHALRHTFGSALAEAGVDLAVMQALLGHAHI
                     DTTARYIHLAPTHVKAEFDAARARQRANT"
     gene            1369..3096
                     /locus_tag="PLP95_07485"
     CDS             1369..3096
                     /locus_tag="PLP95_07485"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HPB45679.1"
                     /translation="MSLLMFLMVQGWLRPGWDWLVAKKLSSFWREVEGSRLEADMSRF
                     CDTAVMVGFTEIQAKRAASQSVGRLLIQTGRPLEALTVGDLDELAAACRAREAATGQG
                     WRHYRSALSCAHIVLFHLDVVGKPPEPAQQPDTFEVRLADCHPNLRPAFVAYLERKLG
                     TCRPKTVSSLATRLAHFGRFLAETDPDLVTLAGLHRQRHIEPYLNSVAHADSAKTGKA
                     ITLADQDRRVRAVGNMLNEITEWGWDDAPTRKLIFRSDHPRKPRPLPRYVPVDADRRL
                     TAALEGSDYRLAADALLLARAVGLRIGELLDLELDCVHEIGGHGAWLKVPLGKLDTER
                     MVPLDDETVALIDRICTTRSVGKPLPHPRTGRPTQFLFTHYGRRLSQTAVRKELARSA
                     QTAGIGHVTPHQLRHTYATALVNAGVSLQALMALLGHVSAEMSLRYAHLFDSTVRAEY
                     ERALDLAKGRIGALPTPTGKTRIPIANNGCGDGDDWRDAPAIKSRLAGGHCLRAPSQG
                     ACPYANICEHCPSFRTDTASIAVLAAQRVDTRALADDAEARGWIDEADRHRRLLDRLD
                     ALIGQAEAG"
     gene            3093..3404
                     /locus_tag="PLP95_07490"
     CDS             3093..3404
                     /locus_tag="PLP95_07490"
                     /inference="COORDINATES: protein motif:HMM:NF040041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6262 family protein"
                     /protein_id="HPB45680.1"
                     /translation="MTTTLEAVEAACTHLAETGDQITFTGVAEHTGISRTTLYRNPQL
                     RAVIDDHRRDSHDRHTLTGLAAEIAHLRTGLEALADRVRDQEERLRHLEGRTTTRRRA
                     N"
BASE COUNT          589 a         1314 c         1062 g          550 t
ORIGIN      
        1 tgcctgttaa ccggccaatg tgatctagct gtagcagcct aagggccggg ttctctgggg
       61 aatgcttgtc ttttggtccg gtcagcgtta cagatgtgtc tgtgtcagac gacgggttgt
      121 tcctcgaacg gggcgacgat ggttggcggc tgggcggcgg cgacgccggc cggttccggt
      181 tggtgaacga gtacttgtcg tatctggcgg accggaacta ctcgccccgc acgacgcgta
      241 cctacgggtt cggtctgctg gcgttctgtc gttggctcga cagcgaagac ctcgacttgg
      301 cctcggtgac gaccgacgtg ttgctgcagt tcctggccgc gtgtcggacc gagctggtca
      361 cgggtcgact gggcggcgcg aacgtggtcg gactcgacgg ccgccggacc gacgtgttgt
      421 ccccggcgac ggtcaacatg cgcctggccg cgatctcggg cttgtactcg ttccggtcga
      481 tgcgcgaccc cgacatctcg aacccggtcc ccaagggtcg ggaagcgaag cgcctgtcgg
      541 cgggggaacg gaccggcatg ctggctcatg tgtcgcgtgc gccgaagcga cggtcaccgc
      601 tgcgggtccg cgacacgaaa cgccttccta agacgctgga tcaccgcgag gtgatccaac
      661 tgttggagag cttgcgcacg tggcgtgacc gggccatcgc cgggctgatg gtgttctgcg
      721 ggctgcggtc agccgaggtt ctcgccctcg atgtcggcga cgtcgacatc ggtgcccgct
      781 ggctacgagt gtcgggcaag ggcggcaagg aacgccgggt cccgctcgac gtcgacgtgg
      841 caggggtgat ccagacgtac ctgctcgccg agcgaccaga cacggccagc tcgcggttgt
      901 tcatcgtggc caagggaccg acccgaggcc agccgctctc gccggcaggg ttgcgacgga
      961 tcttccgtta tcaccgtgac ctgaccggca tcacggctgc gcatccgcac gcgctacggc
     1021 acacgttcgg ctccgcgctg gccgaggctg gtgtcgatct ggccgtgatg caggccctgc
     1081 tgggccacgc gcacatcgac accaccgccc gttacatcca tttggccccc acccacgtga
     1141 aagccgagtt cgatgctgcc cgcgcccgcc aacgcgccaa cacctgacct cgcgccgatc
     1201 ccgtcggccg atgtcatgtt gtcggcgtgg cgggccaacc aggcagcgac cggtcgaggg
     1261 aatccggcat cggactgggc agcacgatcg ttcctgaccc gttggccaca cccgcatgac
     1321 tgggccgacc aatccttggc ggcacggttg gacttggcgc cgtcgaccat gtcactgctc
     1381 atgttcttga tggtccaggg gtggctccgt cccggttggg actggctcgt cgccaagaag
     1441 ctgtcgagct tttggcgtga ggtcgaaggc tctcgcctcg aggccgacat gtcccggttc
     1501 tgcgacaccg ccgtcatggt cgggttcacc gagatccagg ccaagcgggc cgcgtcgcaa
     1561 tcggtcggcc ggctcttgat ccagaccggc cgccccctcg aagcgctcac cgtcggcgat
     1621 ctcgacgaac tcgctgcggc gtgccgggcc cgagaggcag cgaccgggca gggatggcgg
     1681 cactaccggt ccgcactcag ctgcgcccac atcgtgctgt tccacctcga cgtcgtcggc
     1741 aaaccaccag aaccggccca gcaacccgac acgttcgagg ttcggctggc ggactgccac
     1801 ccgaaccttc gcccagcgtt cgtcgcctac ctcgaacgca agctcgggac ctgccgcccc
     1861 aagaccgtct ccagcctcgc tacccgactc gctcacttcg gccggttcct ggccgagacc
     1921 gaccccgacc tcgtcacgct tgccggactg caccgtcagc gccacatcga gccctacctg
     1981 aactcggtcg cccacgccga ctccgccaag accggcaagg cgatcacgct cgctgaccag
     2041 gaccgccgcg tgcgcgccgt cggcaacatg ctcaacgaga tcaccgaatg gggctgggac
     2101 gacgccccga cccggaaact gatcttccgc agcgatcatc cccgcaagcc acggccgcta
     2161 ccccgctacg tgcccgtcga tgccgatcgc cgactcacag ccgcgctcga aggttccgac
     2221 taccggctcg ccgccgacgc cctgttgctg gcccgggccg tcgggctccg catcggggag
     2281 ctgctcgatc tcgaactcga ctgtgtgcac gagatcggcg gtcacggcgc ttggctcaag
     2341 gttccactcg gcaagctcga caccgaacgc atggttcccc tcgacgacga gaccgtggcg
     2401 ctcatcgacc ggatctgcac aacccgctcg gtcggcaaac cgttgcccca cccccgcacc
     2461 ggccgaccga cccagttcct gttcacccac tacggccggc gtctgtccca gacggcggtt
     2521 cgcaaagagc tcgcccgcag cgcccagacc gctgggatcg gccacgtcac tccacaccag
     2581 ctccgccaca catacgccac ggcgctggtc aacgccggcg tgtcgctcca agccctcatg
     2641 gccctgctcg gtcacgtgtc cgccgagatg agcctgcgct acgcgcacct cttcgactcc
     2701 accgtccgag ccgaatacga acgagccctc gacctggcca agggccggat cggcgccttg
     2761 cccacaccca ccggcaagac ccgcatcccg atcgccaaca acggctgcgg tgatggtgat
     2821 gactggcgtg acgcacccgc gatcaagtcc cgtctcgccg ggggtcactg cctccgagcc
     2881 ccgtcccagg gcgcatgccc ctacgccaac atctgcgagc actgcccaag cttccgcacc
     2941 gacaccgcct ccatcgccgt gctcgccgcc caacgcgtcg acacccgggc cctcgccgac
     3001 gacgccgaag ctcgaggttg gatcgacgaa gccgaccgac atcggcgcct cctcgaccgg
     3061 ctcgacgccc tcatcggcca agccgaggcc ggatgaccac aaccctcgag gctgtcgaag
     3121 ccgcctgcac acacctcgcc gagaccggcg accagatcac attcaccggc gtcgccgagc
     3181 acaccggcat cagccgcacc accctctatc gcaaccccca actccgggcc gtcatcgacg
     3241 accaccgccg cgacagccac gaccgccaca ccctcaccgg cctcgccgcc gaaatcgccc
     3301 acctccgcac cggcctcgaa gccctcgccg accgagtccg cgaccaagaa gaacgactcc
     3361 gccacctcga aggccgaacc acaacccgcc gacgagcgaa ctgaccaccc cggatccaca
     3421 ccagagatcg cttcaacccg agccgagatt ggccggttaa tgctgggaca acgccgcagc
     3481 agagtcgttt ttcgggaccc tcaaacgtga gctcg
//
LOCUS       DAOOMA010000247         3509 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_32391_length_3509_cov_4.176028, whole genome shotgun sequence.
ACCESSION   DAOOMA010000247 DAOOMA010000000
VERSION     DAOOMA010000247.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 3509)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3509)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3509
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32391_length_3509_cov_4.176028"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..239)
                     /locus_tag="PLP95_07495"
     CDS             complement(<1..239)
                     /locus_tag="PLP95_07495"
                     /inference="COORDINATES: protein motif:HMM:NF024212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HPB45681.1"
                     /translation="MSRRAETIDLLTQVSRTVRGRTRVALAPLDLSPATARALRTLGR
                     RDGPTRMSDLADALQIGRRSTTDVVELLVSRGFLAR"
     gene            364..2229
                     /locus_tag="PLP95_07500"
     CDS             364..2229
                     /locus_tag="PLP95_07500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015439824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPB45682.1"
                     /translation="MRPGTSSFSADRSVIAHRLAPGTTRRIVAFARPHRRTITIFAVL
                     VVIDAILGAATPLVYKAIVDDAIIPARRDLVVWFATLLGVVALVSAALSLVQRWYSAR
                     LGEQIIVDLRTRVFDHVQTMPIGFFSRAQTGALVSRLNTDVLGAQQAFTSMLSNVVGN
                     VFSVVATLAAMAVLSWQITVLAMVLLPLFILPARWAGKRLGAIKRESHDLNGQLGHLM
                     TERFGVSGALTAKLFGSPEREHRLFAERAERVADIGVAGAMYSRVLMVALGLLASIAT
                     VMLYGVGGWLAIGGSLGIGTLVALTAYVQRLYGPLTSLSNLQVDVMTTLVSFERVFEV
                     LDLKPSIVDPEVPRTLDTAGGLSVELDEVRFRYPTAAEVSLASLEGVAVLESTRNEEV
                     LRGVTIRAGAGERIALVGTSGAGKSTIAQLLTRLYDPTDGAVRLGGIDLRDLTQRDIR
                     SAVAMVAQDVHLFHDTLAANLRYAKPDATNAELETALRAARIWDVVEAMPAGLDTVVG
                     DRGHRLSGGEKQRISIARVLLKDPAVVVLDEATAHLDNNVEAEVQAALDDALSGRTSV
                     VIAHRLSTVRDADRIYVLEAGEVVESGTHAELLSVDGPYARLAANATLGPLVPAA"
     gene            complement(2249..3262)
                     /locus_tag="PLP95_07505"
     CDS             complement(2249..3262)
                     /locus_tag="PLP95_07505"
                     /inference="COORDINATES: protein motif:HMM:TIGR03559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HPB45683.1"
                     /translation="MKLGLTWGYWGALPPGNLVELTQEAERLGFDSVWSAEAWGSDAF
                     SPLAYLAAHTSTIKLGTGVVQLSGRTPTAAAMHAITLDHLSQGRLILGLGVSGPQVVE
                     GWYGQAYRKPLTRTREYVEIIRRVLAREEPLEFQGEFHQHPFRGEGSTGLGKPLKAIT
                     HPYRRDIPIFLGAEGPKNVAMTAEIADGWLPLYYSPFRPEVYAEHLDGAKDGFEVTAM
                     ANITITDDLEAGLYPVKAALGFYIGGMGAKGKNFHTDLMARMGYEEEAYRIQDLFFEG
                     RRDEAIAMVPDQFADEISLVGPPGRIKERLQEWKNSPVTTVTMYPSSPDQLRQAAEII
                     LGD"
     gene            complement(3296..>3509)
                     /locus_tag="PLP95_07510"
     CDS             complement(3296..>3509)
                     /locus_tag="PLP95_07510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HPB45684.1"
                     /translation="KAKEIAAAICANGPLAVQALLRTLRETNGMSEAEALDHEFTYGW
                     DVFATEDAKEGAVAFKEKRDPVYKGK"
BASE COUNT          532 a         1147 c         1212 g          618 t
ORIGIN      
        1 cgagcgagga acccgcgcga caccaggagc tcgacgacat cggtggtgct gcgccgcccg
       61 atctggaggg cgtcggcgag gtcgctcatc cgtgtcggcc cgtctcggcg gccgagagta
      121 cgcagggctc gggctgtcgc tggcgagagg tcgagcgggg ccaaggcgac gcgggttcgg
      181 ccccggaccg tacgtgacac ctgtgtgagc aggtcgatgg tttcggcacg tcgtgacacg
      241 ggggctccgg ccggtcggga atacgcgggc aggtagtgag gttacctctc catacgctgg
      301 ccacgtcgcc tggcacgacc tggctgaacg agtcggcgga cctcgccgga agggagtgaa
      361 agtatgcgac caggaacctc ctcattcagt gcggatcgct ccgtcatcgc ccaccggctg
      421 gccccgggta cgacccgtcg catcgttgcg ttcgcccgtc cccatcgccg aacgatcacg
      481 atctttgcgg tcttggtcgt catcgacgcg atcctcggcg ccgcgacacc actcgtgtac
      541 aaggcgatcg tggacgacgc catcatcccg gcgcgacgcg acctcgtggt gtggttcgcc
      601 acgttgctcg gcgtggtcgc gctcgtcagt gccgcgctca gcctcgtgca acgctggtac
      661 tccgcccgcc tcggcgaaca gatcatcgtc gacctgcgaa cgcgggtatt cgaccacgtc
      721 cagacgatgc cgatcggatt cttctcgagg gcacagaccg gggcactcgt cagccgactc
      781 aacaccgacg tgctcggcgc gcagcaggcc ttcacctcga tgctgtccaa cgtcgtcggc
      841 aacgtcttca gcgtggtcgc gaccctggcg gcgatggcgg tgctgagctg gcagatcacc
      901 gtcctggcca tggtgctgtt gccgctgttc attctcccgg cacggtgggc gggtaagcgg
      961 ctcggggcga tcaagcggga gagccacgac ctcaacgggc aactggggca tctgatgacc
     1021 gagcgcttcg gggtgtcggg tgcgctcacc gccaaactct tcgggtcacc ggagcgtgaa
     1081 caccggctgt ttgccgagcg cgccgaacgc gtcgccgaca tcggtgttgc cggagcgatg
     1141 tattcgcggg tgctgatggt ggcgctcggg ctgttggcat cgatcgccac ggtcatgttg
     1201 tacggcgtcg gagggtggtt ggccatcggc gggtcgctcg gaatcggcac gctcgtcgcg
     1261 ctcactgcgt atgtccagcg gctctatgga ccgttgacct cactgtcgaa tctgcaggtc
     1321 gacgtcatga ccacgttggt gagcttcgag cgggtctttg aggtgctgga cctgaagccc
     1381 agcatcgtgg acccggaagt gccgcgcacg ctcgacaccg ccggcgggct cagcgtcgaa
     1441 ctcgacgagg tgcgcttccg ctatccgacc gcggccgagg tgtcgctcgc ctcgttggag
     1501 ggggtcgcgg tcctcgagtc gacgcgaaac gaggaggtgc tgcgcggcgt cacgattcgc
     1561 gccggtgcgg gtgaacgcat cgcgctggtc ggtacgagcg gcgcgggaaa gtcgacgatc
     1621 gctcaactcc tcacccggct ctacgatccg accgacggtg cggtccggct cggcggcatc
     1681 gaccttcgcg acctcaccca acgcgacatc cgatcggcgg tggcgatggt cgcccaggac
     1741 gtacacctct tccacgacac gttggccgcc aaccttcgct acgcgaagcc cgatgcgacg
     1801 aacgccgagc tggagacggc gctgcgtgcg gcacggatct gggacgtggt cgaggcgatg
     1861 cccgcggggc tcgacaccgt ggtgggtgac cgcggccatc gtctcagcgg cggcgagaag
     1921 cagcgcatct ccatcgcccg ggtgctgttg aaggaccctg cggtcgtcgt gttggacgag
     1981 gcgaccgcac acctcgacaa caacgtcgag gccgaggtcc aagcggcact cgacgacgca
     2041 ctttcggggc gtacctcggt cgtgatcgcg caccgcctgt ccacggtgcg cgatgccgac
     2101 cggatctacg tgctcgaagc gggggaggtg gtggagtccg gcacccacgc cgaacttctc
     2161 agtgtggatg gcccctatgc gcgcctcgcc gccaacgcga ccctgggtcc gctcgtcccc
     2221 gcggcatgat cgcggcatga ccgcggcgtc agtctccgag aatgatctcg gcggcctgcc
     2281 ggagttggtc gggcgaggac ggatacatcg tcaccgtggt gacgggtgag ttcttccatt
     2341 cctgcagccg ctctttgatg cgcccgggcg ggccgacgag tgagatctca tccgcgaact
     2401 ggtcgggcac catggcgatg gcctcgtcgc gacggccttc gaagaagagg tcctggatcc
     2461 ggtaggcctc ctcctcgtat cccatgcggg ccatgaggtc ggtgtggaag ttcttgccct
     2521 tcgctcccat gccaccgatg tagaacccga gcgcggcctt gaccggatac agcccggcct
     2581 cgaggtcgtc ggtgatcgtg atgttcgcca tcgccgtgac ctcgaacccg tccttggccc
     2641 cgtcgaggtg ctccgcgtag acctcggggc ggaacggcga gtagtagagc ggcagccaac
     2701 cgtccgcgat ctcggccgtc atcgccacgt tcttcggccc ttcggccccg aggaagatcg
     2761 ggatgtcacg ccggtacgga tgcgtgatgg ccttgagcgg tttgccgagc ccggtagatc
     2821 cctcgccgcg gaacgggtgc tggtggaact cgccctggaa ctcgaggggc tcctcgcggg
     2881 cgagcacccg gcgaatgatc tcgacgtact cgcgggtgcg ggtgagcggt ttgcgatacg
     2941 cctggccgta ccacccctcg accacctggg gtcccgagac accgagcccg aggatgagcc
     3001 gaccttggga gaggtggtcg agggtgatgg cgtgcatggc cgcggcggtg ggggtgcggc
     3061 cggacaactg cacaactccg gtgcccagct tgatggtcga ggtgtgcgcg gccaggtagg
     3121 cgaggggact gaacgcgtcg gatccccacg cctcggccga ccacaccgaa tcgaagccga
     3181 gacgttcggc ctcctgggtc aactcgacga ggttgccggg cggcaacgcg ccccagtacc
     3241 cccaggtcag tccgagcttc atgtgtttgc ctccggatcg tgcggaccgg tcagatcact
     3301 tgcccttgta caccgggtcg cgcttctcct tgaacgcgac ggcgccctcc ttcgcgtcct
     3361 cggtggcgaa gacgtcccac ccgtaggtga actcgtggtc gagggcctcg gcctcgctca
     3421 tgccattggt ttcgcggagg gttcgcaaga gggcctgcac cgcgagcggt ccgttcgcgc
     3481 agatcgcagc ggcgatctcc ttggccttg
//
LOCUS       DAOOMA010000248         3502 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_32476_length_3502_cov_3.107630, whole genome shotgun sequence.
ACCESSION   DAOOMA010000248 DAOOMA010000000
VERSION     DAOOMA010000248.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 3502)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3502)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3502
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32476_length_3502_cov_3.107630"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..891)
                     /gene="tig"
                     /locus_tag="PLP95_07515"
     CDS             complement(<1..891)
                     /gene="tig"
                     /locus_tag="PLP95_07515"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HPB45685.1"
                     /translation="MKSSIEPQEGNKVKISVTIEAEEFEPAIAAAWQAIAREVRIPGF
                     RPGKAPRKVLEARIEPGYARSEAINKAVPEFYFRAVREHDVDAIDQPDVEVTGGEEDG
                     DVTFDAVVEVRPEISLSGYEGLTIEVPNPIPSDDDVTEQIDRLRGQYGDLVAVERPVQ
                     RDDYVTIDLSAKQGDEVVEGLTVDNYSYVVGSGVIVPELDDHLVGAEVDNELSFTAPH
                     PREGEDPVDFSVTVRGVKEQQLPDLTDAWVSDATEFGSVADFRDDVVERLSAARTSEA
                     SVAVQNGPGDKLADLVLDEVP"
     gene            1005..1082
                     /locus_tag="PLP95_07520"
     tRNA            1005..1082
                     /locus_tag="PLP95_07520"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1040..1042,aa:Pro,seq:tgg)
     gene            1105..1179
                     /locus_tag="PLP95_07525"
     tRNA            1105..1179
                     /locus_tag="PLP95_07525"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1138..1140,aa:Gly,seq:tcc)
     gene            1283..1864
                     /locus_tag="PLP95_07530"
     CDS             1283..1864
                     /locus_tag="PLP95_07530"
                     /inference="COORDINATES: protein motif:HMM:NF013346.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM165/GDT1 family protein"
                     /protein_id="HPB45686.1"
                     /translation="MSSALTAFAAVFPAELPDKTMFATIVLTTRYRRPLAVWVGSAAA
                     FAMHVTIAVAAGGLLAKLPDRVVNVVVFFMFAAGSAFLLREALSGRDEEEHDLGATGD
                     TSAKAAALAAFAMIGLAELGDLTQLTTASLAARTGDPFGVALGAFAALLAVSGLAATF
                     GSKLTERFSLDKLNYVGAAVFAGFALWTLLDII"
     gene            complement(1895..2809)
                     /locus_tag="PLP95_07535"
     CDS             complement(1895..2809)
                     /locus_tag="PLP95_07535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012224376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HPB45687.1"
                     /translation="MGHGHDHHHATAARGGQRHRRPLAIALALTVAFLVVQVVVGFAT
                     GSLALISDAGHMATDSLGLAMALTAIVVASRGSSRSTHTFGTYRLEILAALANAVLLF
                     GVAGYVLYEAAVRLGDPPEVASMPVLVVGVIGLGVNVAAFALLRAGAKESLNIEGAYV
                     EVLSDALGSLGVIVAAVVMHVTGWRWVDAAIGSAIGLFILPRAWRLGAEALRVLLQVA
                     PGHIDIDELRRDLAALDGVVDVHDLHLWTLTSEMEVLTAHLRTAPGADAHSVLDDARS
                     MLAAKHGLDHATLQVEPLDHEGCDAVDW"
     gene            complement(2854..3282)
                     /locus_tag="PLP95_07540"
     CDS             complement(2854..3282)
                     /locus_tag="PLP95_07540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="globin"
                     /protein_id="HPB45688.1"
                     /translation="MATSPLGMPRGVNEPTVYDTVGGEAFFRELVDHFYDNVEADEPL
                     VALYPERDDLGPARERFRLFLSQFFGGPRTYTDTRGHPRLRMRHLPFTVDEDGRDRWL
                     RAMRSAMDVMDIDPAIDATMWEYFVQSAEFMRNDRIPEQD"
     gene            3359..3432
                     /locus_tag="PLP95_07545"
     tRNA            3359..3432
                     /locus_tag="PLP95_07545"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3393..3395,aa:Arg,seq:tct)
BASE COUNT          555 a         1170 c         1167 g          610 t
ORIGIN      
        1 gggtacctcg tcgagcacga ggtcggcaag cttgtcgccg gggccgttct gcacggcgac
       61 cgatgcctcg gacgttcgcg cggcgcttag acgctcgacc acatcgtcgc ggaagtcggc
      121 cacggacccg aactcggtgg cgtcgctcac ccacgcatcg gtgaggtcgg gaagctgttg
      181 ttctttgacc ccgcgcaccg ttaccgagaa gtcgaccggg tcttcgccct cgcgcggatg
      241 cggtgcggtg aagctgagtt cgttgtcgac ctcggcgccc acgaggtggt cgtcgagttc
      301 ggggacgatc actccggaac caacgacata gctgtagttg tcgacggtga gaccttcgac
      361 gacctcgtcg ccctgctttg cggacaggtc gatcgtgacg taatcgtcgc gctgcaccgg
      421 acgctcgacc gcgaccaggt cgccgtactg gccacggagg cgatcgatct gttcggtcac
      481 gtcgtcgtcg ctcggaatcg ggttggggac ctcgatcgtc aggccttcat agccgctgag
      541 ggagatctct gggcgcacct cgaccacggc gtcgaaggtg acgtcgccgt cttcctctcc
      601 gccggtcacc tcgacgtcgg gctgatcgat ggcgtcgacg tcgtgctcgc gcacggcccg
      661 gaagtagaac tccggcacgg ccttgttgat ggcctcgctg cgggcatacc caggctcgat
      721 ccgagcctcg agcaccttgc gaggggcctt gcccggacgg aacccgggga tacggacctc
      781 tcgggcgatg gcctgccagg cggcggcgat cgccggctcg aactcttccg cctcgatcgt
      841 gaccgagatt ttgaccttgt tgccctcttg gggctcgatg gaggacttca cagggacgga
      901 agcttactct gtcgaccgag gggcgccgga atcggccgcc tgcgacggat tccgttcgcg
      961 acgtcctcgg tcatgtacgg taggcgcggc ccgttcgggg ccatcgggac gtggcgcagt
     1021 ctggctagcg caccggcttt gggagccggg ggtcgagagt tcgaatctct ccgtcccgac
     1081 caaccgctat agtggccaac tcctgcgggt gtagctcaat ggctagagcc cctgccttcc
     1141 aagcaggcta tgcggtttcg attaccgtca cccgctccat cgaaacggcg tgaccggttg
     1201 gtggttggct gagatcctcg gcgaaccacc aaccggtcgc cggcctcgaa actcccgcac
     1261 cgaccaccat cggggatgca aaatgagttc cgccctgacg gcgttcgccg cggtgtttcc
     1321 cgccgagcta cccgacaaga cgatgttcgc cacgatcgtg ttgacgacgc gatatcgacg
     1381 gcccctcgcc gtgtgggtgg gttcggcggc ggcgttcgcg atgcacgtca cgatcgccgt
     1441 agccgccggc ggcctgctcg ccaaactgcc cgaccgggtc gtcaacgtcg tcgtgttttt
     1501 catgttcgcc gcaggttcgg cattcctgct gcgcgaggcc ctgtcgggcc gcgacgagga
     1561 ggaacacgat ctcggcgcca ccggtgacac ctcggccaag gcggcagcac tcgcggcgtt
     1621 cgccatgatc gggctcgcgg aactcggcga tctcacccag ttgaccaccg catcgctcgc
     1681 agcgcgcacc ggcgacccgt tcggcgtggc cctcggagcg ttcgctgcgc tgttggcggt
     1741 gtctgggctt gccgccacct tcgggtcgaa gctcaccgag cggttcagcc tcgacaagtt
     1801 gaactacgtc ggggccgccg tgttcgccgg gtttgctctc tggacgttgc tcgacatcat
     1861 ctgaacgacg tgcctcgacc gagctatgcg atcttcacca atcgaccgcg tcacagccct
     1921 cgtggtcgag cggctcgacc tgaagggtcg cgtgatcgag cccgtgtttg gccgcgagca
     1981 tcgagcgggc gtcgtcgagc accgagtgag cgtcggcccc gggggcggtg cggaggtgtg
     2041 cggtcaacac ctccatctcc gaggtcaggg tccacaggtg caggtcgtgc acgtccacca
     2101 cgccgtccag cgcggcgagg tcacggcgca gttcgtcgat atcgatgtgg ccgggggcga
     2161 cctggagcag cacccgcaac gcctcggcac cgagacgcca ggcccgcggc aggatgaaca
     2221 gcccgatggc gctgccgatc gccgcgtcga cccacctcca tccggtgacg tgcatcacca
     2281 ccgccgccac gatcacgccg agcgatccca acgcgtcgct caacacctca acataggcgc
     2341 cctcgatgtt gagcgattcc ttggcgccgg cgcgcaggag cgcgaacgcc gcgacgttga
     2401 cgccgaggcc gatcaccccg acgaccagga ccggcatcga ggcgacctcc ggtgggtcgc
     2461 ccaaccgaac cgccgcctcg tagagcacat agcctgcaac gccgaatagc agcacggcgt
     2521 tggccagcgc cgcgaggatc tcgagacggt aggtgccgaa ggtgtgcgtt gagcggctgc
     2581 tgccccgcga cgcgaccacg atcgcggtga gcgccatcgc cagccccagc gaatcggtgg
     2641 ccatgtgacc ggcgtcggag atgagcgcca acgatccggt ggcgaacccg accacaacct
     2701 gcacgacaag aaacgcgacg gtcagcgcta acgcgatcgc gagcggccgg cggtggcgct
     2761 gcccgccacg tgccgcggtc gcgtggtgat ggtcgtgacc gtgacccatc cgagcctcct
     2821 cgctgttgtg tggacgccgc cggtggggcg ggatcagtcc tgttccggga tgcggtcgtt
     2881 gcgcatgaac tcggcgctct gcacgaagta ctcccacatc gtcgcatcga tggccgggtc
     2941 gatgtccatg acatccatcg ccgaacgcat cgcccgtagc caacggtcgc gaccgtcctc
     3001 atcgacggtg aagggcaggt ggcgcatccg caggcgcggg tggccgcggg tgtcggtgta
     3061 ggtgcgcggc ccgccgaaga actggctcag gaacaggcgg aatcgttctc gggcagggcc
     3121 gaggtcgtcg cgttctgggt acaacgcgac cagcggctcg tcggcctcga cgttgtcgta
     3181 gaagtgatcg acgagctcgc gaaaaaacgc ttcccctccg acggtgtcat agacggtggg
     3241 ttcgttcacc ccacggggca ttccgagtgg actcgtggcc atcacccgac actactgccc
     3301 ggcccgggcc tggccacgag gcgtcgccgg gtgaccgctg agtacacttg tcacgcctgc
     3361 ctccgtagct cagttggata gagcgggagc cttctaatct ctaggtcgta ggttcgattc
     3421 ctaccggggg cgcaacgcgg cgattcgacc acggcgtaat tggtcgccgt ggtcgaatcg
     3481 ccgcgttgcg cccccggtag ga
//
LOCUS       DAOOMA010000249         3490 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_32632_length_3490_cov_4.189229, whole genome shotgun sequence.
ACCESSION   DAOOMA010000249 DAOOMA010000000
VERSION     DAOOMA010000249.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 3490)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3490)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3490
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32632_length_3490_cov_4.189229"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..656
                     /locus_tag="PLP95_07550"
     CDS             <1..656
                     /locus_tag="PLP95_07550"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HPB45689.1"
                     /translation="VAYLAAALATVVRERDVAATERRLRHEATHDHLTGLLNRAGLAS
                     LAQEAVGDPGAATRLIGFIDLDGFKAINDTLGHEVGDAVLHAVGLRLDRLFGEDHWLA
                     RLGGDEFVVVSPTSPGVTEDEFSASVERIFSDPVATAKGSVEARGSVGVARFDPALDP
                     SQQWRRADAEMYAAKAARKAQRHDAQRAASGVVERGDRGGHVERDKSALLVPLDERV"
     gene            707..1996
                     /locus_tag="PLP95_07555"
     CDS             707..1996
                     /locus_tag="PLP95_07555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB45690.1"
                     /translation="MKNYLGAAALTAAVALVGCTPSNPGTTTTVPTGPTADRFVVNGA
                     ADVGDCATLATACGTVNYAVSQAADGETISVGAGTYDEMVSVDKSLTFEGPNAGIGAG
                     TAAGTRGAEATVKGFRSPGNPHPVAAYEFDVTIDGFSIDPQGDSSLLTPDTLNLIALY
                     GGSDVEIVNNRLNGGAWNPLCSYSCTTMADSAILVRSGSYLVSNNRVENFRRPLDISQ
                     ASPATPVVSAKYTKNVVQNFSFRGFWAAEWDNSAFGANTISIDDNYIEGGSTADLTAN
                     PAFSPTAMLVTAGGVDFTNNTVKNVDTGIYEEVCGVPNPNDLVNRYTGNTFDELRAGL
                     TIATAGDCTGRTADRVFTGNSFVGGVWDRTGNPTRAAAWFVNGVIPADASGAPGTIDA
                     ACTFWGSADGPGVNPNPWVTPGVSTTPFRAALGDACP"
     gene            2075..3211
                     /locus_tag="PLP95_07560"
     CDS             2075..3211
                     /locus_tag="PLP95_07560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB45691.1"
                     /translation="MKRRLTAAEIATVEAASWVTSHLRRRLDALDTTKVRWSRERAWE
                     WYERTPWLVGANFVPSTAGNQLEMFQAATFDSVTIDRELGWAADLGMNSMRIFLHDLL
                     WETEGSGFLDRVDEVLSIAASHGIGVMPVLFDGIWDPRPQAGPQGDPKPGVHNSMWLQ
                     SPGASVISDRRQWARLRGYVEAVMGRFATDDRVQVWDLFNEPDSPNPAYAFHEARHKQ
                     RLVAELLEQIWDWADGVDPDQPVTAGLFMHTLRAVEARPVSKVMLERSDVISFHSYQP
                     RHQLVEIIDRLEGFGRPLLCTEWMGRPTSPVSLLEVFEQRRVGCWTWGLVDGRTQTNY
                     PWTSWFTRYDPEVDGWFHDLLRPDGTPYDTDEAALFRRVTAVYR"
BASE COUNT          594 a         1115 c         1171 g          610 t
ORIGIN      
        1 tcgtggccta tcttgccgcc gcgctcgcga ccgtcgttcg agagcgcgac gtcgcggcga
       61 ccgagcgtcg cttgcgccac gaggcgaccc acgaccatct caccgggttg ctcaaccggg
      121 ccgggctcgc gtcgctggct caagaggcgg tcggcgaccc cggtgcggca acgcgactga
      181 tcggattcat cgacctcgat gggttcaagg cgatcaatga caccctcggc catgaggtcg
      241 gcgatgccgt gctgcacgcg gtcgggctcc gactcgatcg gctcttcggg gaggatcact
      301 ggcttgcccg tctcggcggc gacgagttcg tcgtcgtgag cccgaccagc cccggggtga
      361 ccgaggacga gttctcggcg tcggtcgagc ggatcttcag cgacccggtg gcgaccgcga
      421 agggttcggt ggaggctcgc ggttcggtgg gtgtggcgcg gttcgacccg gcgctcgacc
      481 cctcgcagca gtggcgtcga gccgatgcgg agatgtacgc cgccaaggcg gcgcgcaagg
      541 cgcaacgcca cgacgcccag agggccgcgt cgggtgttgt cgagcgtggt gaccgtggtg
      601 gccatgtcga acgggacaaa agtgcattgc ttgttccttt ggacgaacgg gtgtaaacat
      661 gagacctcat tcgggtatcc ggggggatac ccagaaatgg ggagagatga agaactatct
      721 aggcgcggcc gcgttgacgg ctgcggtggc gctcgtgggg tgcaccccga gtaatcccgg
      781 aacgacgacg accgtgccca cgggcccgac cgccgaccgt ttcgtcgtca atggcgccgc
      841 tgatgtcggc gactgcgcaa cgttggccac ggcgtgtgga acggtgaact acgccgtgtc
      901 gcaggccgct gatggtgaga ccatctcggt tggggcaggc acctacgacg agatggtgag
      961 cgtcgacaag tcgctcactt tcgaggggcc gaacgccggc atcggcgccg ggaccgcagc
     1021 gggcacgcga ggcgcggagg ccaccgtcaa ggggttccgc agccccggaa acccgcaccc
     1081 ggttgccgca tatgagttcg acgtcacgat cgacgggttc tcgatcgacc cgcaaggcga
     1141 cagctccctg ctcacgccgg acactttgaa cctcatcgcc ctctacggcg gcagcgacgt
     1201 cgagatcgtc aacaaccgcc tcaacggcgg cgcctggaat ccgctgtgca gctactcctg
     1261 caccacgatg gcggacagtg cgatcctcgt acgttcgggt tcgtacctcg tttcgaacaa
     1321 ccgggtggag aacttccgtc gcccactcga catctcacaa gcgagtccgg ccacccccgt
     1381 cgtctcggcc aagtacacca agaatgtcgt gcagaacttc tcgttccgcg ggttctgggc
     1441 ggcggagtgg gacaactcgg ccttcggcgc caacacgatc agcatcgacg acaactacat
     1501 cgagggaggc agcactgccg acctcaccgc caacccggcc ttcagtccga ccgcgatgct
     1561 cgtgaccgca ggcggtgtcg acttcacgaa caacaccgtg aagaacgtcg ataccggcat
     1621 ctacgaggag gtgtgcggcg tcccgaaccc gaatgatctc gtcaaccgct acacgggcaa
     1681 cacctttgac gaactccgtg ctgggctcac gattgccacg gccggcgact gcaccggacg
     1741 tacggcggat cgcgtcttca ccgggaactc gttcgtgggt ggagtgtggg accgcacggg
     1801 caacccgacc cgcgccgcgg cgtggttcgt caacggggtc atcccggctg acgcttctgg
     1861 agctcccggc acgatcgacg ctgcgtgcac cttctgggga tcggccgacg ggccgggtgt
     1921 gaacccgaac ccctgggtca ccccgggcgt ctccaccaca ccgttccgcg ctgcgctggg
     1981 cgatgcctgc ccctgatcgg ctgtcaccag gccggtgatg ccggcctggt gattccggcg
     2041 cgaccggtcc tcggttgtgc gccaaaatgc agcgatgaag cgacgcctca ccgctgccga
     2101 gattgcgacc gtcgaggcgg cctcgtgggt gaccagtcac cttcggcgcc gcctcgacgc
     2161 gttggatacg acgaaggttc gttggtctcg ggagcgggcg tgggagtggt acgagcgcac
     2221 cccgtggctc gtcggcgcga acttcgtgcc ctcgacggcg ggtaaccagc tcgagatgtt
     2281 ccaggcggcg accttcgatt cggtcacgat cgatcgtgag ctcggttggg cggccgacct
     2341 cggcatgaac tcgatgcgca tcttcctgca cgacctgttg tgggagaccg aagggagtgg
     2401 attcctcgat cgtgtcgacg aggtgttgtc gatcgctgcg tcccacggca tcggagtgat
     2461 gcccgtgctt ttcgatggca tctgggatcc tcgcccccag gccggacctc agggcgatcc
     2521 gaagccgggt gttcacaact cgatgtggct gcaatcgccc ggagcgtcgg tgatctcgga
     2581 ccgccgacag tgggcgcggc ttcgcggcta cgtcgaggcg gtgatgggac ggtttgcgac
     2641 ggatgatcgc gtacaggtgt gggacctgtt caacgagccc gacagcccca acccggcgta
     2701 cgcattccat gaggcgcgcc acaaacaacg actcgttgcc gaactcctcg aacagatctg
     2761 ggactgggcc gacggcgtcg accccgacca gccggtcacc gctggtctgt tcatgcacac
     2821 gttgcgcgcc gtcgaggctc gaccggtctc caaggtgatg ctcgagcgtt cggacgtgat
     2881 ttcctttcac agctatcagc cgcgccatca gctcgtcgag atcatcgacc gcctcgaggg
     2941 gttcgggcgg ccattgctgt gcaccgaatg gatggggcgc ccgacatcgc cggtgtcgtt
     3001 gctcgaggta ttcgagcaac gacgggtcgg gtgttggacc tggggtctcg tcgacggccg
     3061 tacacagacg aactacccgt ggacctcgtg gttcacgagg tacgaccccg aggtggacgg
     3121 ctggttccac gacctgctgc gacccgatgg aacgccctat gacaccgacg aggcggcgtt
     3181 gtttcggcgg gtcacggcgg tctaccgctg agatcggttc cgtcccgttt cgccgatgcg
     3241 agcgtccggg aaacacagcg gtccgggcgc cgaacaacac ccggaccgaa tcaccatccc
     3301 gaggttggcg cagcacccct acggcgccaa ccccggaact cgtgtgtgaa cccagcagag
     3361 cggctctgtt aggtggggct atcattgcat atcaggatga gttgtcaagc tggttggtga
     3421 actcttcccg aaactgcgat ggccaatggt cgcgatgggt agggacaggg atcggtaggg
     3481 ttcgaccatg
//
LOCUS       DAOOMA010000250         3490 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_32636_length_3490_cov_3.419505, whole genome shotgun sequence.
ACCESSION   DAOOMA010000250 DAOOMA010000000
VERSION     DAOOMA010000250.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 3490)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3490)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3490
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32636_length_3490_cov_3.419505"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..577)
                     /locus_tag="PLP95_07565"
     CDS             complement(<1..577)
                     /locus_tag="PLP95_07565"
                     /inference="COORDINATES: protein motif:HMM:NF024968.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HPB45692.1"
                     /translation="MNAAANAPASASASSAPASASASSASASSGSRPRLIHLTTADIS
                     LELLLGPQLSAFAQAGYEVIAMSAPGPFVPAVEARGIRHVALDNATRSMDLFADVRAL
                     RELVRQFRTLRPDIVHTHNPKTGVYGRIAAKIARVPVIINTVHGLYATPEDRLVRRVA
                     VYGAERIAAICSDRELVQNVEDIATLRSLRIP"
     gene            complement(574..1398)
                     /locus_tag="PLP95_07570"
     CDS             complement(574..1398)
                     /locus_tag="PLP95_07570"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HPB45693.1"
                     /translation="MGESSFPRRALKATAHLADRLSTPERGIVIAIYHRVGAAGGGQM
                     NLDPAVFTDQIAWLRDNRRIISLDQAVAELAAARADATPLEPAVVLTFDDGTADWLDV
                     VAPILVAHDAPATFYLTTAYTDGTQPLPDGEAALSWTGVEELAATGIATIASHTHTHV
                     LLDRLAPAAIADELDRSIDLIGEHLGATPRHFAYPKALPPSAAADAAVRERFDSAVLA
                     GTRANTATADLHQLSRSPIQAADDERDARAKFDGGLGFEDALRRRINTIRYRGKNR"
     gene            complement(1400..2341)
                     /locus_tag="PLP95_07575"
     CDS             complement(1400..2341)
                     /locus_tag="PLP95_07575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020378535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HPB45694.1"
                     /translation="MKVVITGGAGFIGANLARIMSNTDEITEVVAFDNLSTGFRTNLD
                     GLDTSVVRLVEGDILNPDELDAAIAGASAVVHLAARPSVPRSVKDPVASHHANATGTL
                     NVLEAARRAGDVQVIVASSSSVYGSNPELPKHEDLATRPLSPYAASKLATEAYALAYG
                     SSYGMATLAFRFFNVFGPLQAAGHAYAAVIPAFVDAALAGRPLPVHGDGTQSRDFTYV
                     DTVASVITDAVLRRVSSPEPVNLAFGSRTNLLEVIELLKGELGTDLAVDFQPSRTGDV
                     PHSQAANDRLRALFPDIEPVDLATGLTRTVEWFRTLA"
     gene            complement(2542..3213)
                     /locus_tag="PLP95_07580"
     CDS             complement(2542..3213)
                     /locus_tag="PLP95_07580"
                     /inference="COORDINATES: protein motif:HMM:NF016167.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF433 domain-containing protein"
                     /protein_id="HPB45695.1"
                     /translation="MPNTVDLLDRPTYGYGEVDRLLHLRAGTSKRWIDGYTRGGKQYA
                     PVVREHPTDSPYVTWGEFVETRYLSEFRNAGVSMQQMRPAVALLRQRFQTKYPLAHAR
                     PFTDGRDLVLKVQEEVGLDHRLSLVVVASGQLKLSTGAQTFYSTTEFDPDRDNTAVLI
                     RPLATSASVTIDPYRNSGSPAVRGVRTEVIFEQFKTGEEIEAIADTFDLTVSEVEDAL
                     RYEAA"
     gene            3271..>3490
                     /locus_tag="PLP95_07585"
     CDS             3271..>3490
                     /locus_tag="PLP95_07585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB45696.1"
                     /translation="MVVGVVSGLDAAFVQRWHLELAAAGLVLGVVVALAMWLSRSVMP
                     RACGDLEVWLPVLFTLTLVGLWATVPDTE"
BASE COUNT          520 a          950 c         1397 g          623 t
ORIGIN      
        1 cggggatgcg aagggatcgc agcgtggcga tgtcttcgac gttctggacg agttctcgat
       61 ccgaacagat ggcggcgatg cgttcggctc cgtagacggc gacccggcgc acgaggcgat
      121 cctccggggt ggcgtagagg ccgtgcaccg tgttgatgat gacggggact cgggcgatct
      181 tggcggcgat gcgtccgtac acgccggtct tggggttgtg ggtgtggacg atgtcggggc
      241 gcagggtgcg gaattggcgc acgagttcgc gcagggcccg aacgtcggcg aacaggtcca
      301 tcgaccgggt ggcgttgtcg agggcgacgt ggcggatgcc gcgggcctcg acggcgggca
      361 cgaaggggcc gggggccgac atggcgatga cctcgtagcc cgcctgggcg aaggcagaga
      421 gttgagggcc gaggaggagt tcgagactga tgtcggcggt ggtgaggtgg atgaggcgcg
      481 ggcgcgaacc cgacgacgcc gacgcgctcg acgcgctcgc cgaggctggc gcgctcgacg
      541 cgctcgctga ggctggcgcg ttggccgcag cgttcaccgg ttcttgcccc ggtagcggat
      601 ggtgttgatg cggcggcgca gggcgtcttc gaatccgagt ccgccgtcga atttggcgcg
      661 ggcgtcgcgc tcgtcgtcag cggcttggat gggcgaacgc gacagttggt gcagatccgc
      721 ggtggcggta ttggcgcggg tgccggcgag tacggccgag tcgaagcgtt cgcgcaccgc
      781 agcgtcggcg gcggccgatg gtgggagcgc cttgggatag gcgaagtggc gtggtgtggc
      841 gccgaggtgt tcgccgatga ggtcgatgct gcggtcgagt tcgtcggcga tggcggccgg
      901 cgcgagacgg tccaacaaca cgtgggtgtg ggtgtggctg gcgatggtgg cgatgccggt
      961 ggcggcgagt tcctcgaccc cggtccagct cagcgcggcc tcgccgtcgg ggaggggttg
     1021 ggtgccgtcg gtgtaggcgg tggtgagata gaaggtggcg ggggcgtcgt gggcgacgag
     1081 gatgggggcg acgacgtcga gccaatcggc ggtgccgtcg tcgaaggtca acacgacggc
     1141 cggttcgagg ggggtcgcgt cggctcgagc cgcggcgagt tcggcgacgg cctggtcgag
     1201 ggagatgatg cggcggttgt cgcgcagcca cgcgatctgg tcggtgaaca cggccggatc
     1261 gaggttcatc tgaccgccgc ccgcggcgcc gacgcggtgg tagatggcga tgacgatgcc
     1321 gcgctcgggc gtggagagcc gatcggcgag atgcgcggtg gccttcagcg ctcggcgcgg
     1381 aaacgagctc tcgcccatgt caggcgaggg tgcggaacca ctccacggtg cgggtgagtc
     1441 cggtggcgag gtcgacgggt tcgatgtcgg ggaacagggc tcggaggcgg tcgttggcgg
     1501 cctgggagtg ggggacgtcg ccggtgcgcg agggttggaa gtcgacggcg aggtcggtgc
     1561 cgagttcacc tttcaacagt tcgatgacct cgagcaggtt ggtgcgcgag ccgaacgcga
     1621 ggttgaccgg ttcgggcgag gaaacccgcc gcaggacggc atcggtgatg accgaggcga
     1681 cggtgtcgac gtaggtgaag tcgcgcgact gggtgccgtc gccgtgcacc ggaagggggc
     1741 gaccggcgag ggcggcgtcg acgaaggccg ggatgaccgc ggcatacgcg tggccggcgg
     1801 cttggagggg gccgaacacg ttgaagaagc ggaaggccaa cgtcgccatg ccgtagctcg
     1861 acccgtaggc gagggcgtag gcctcggtgg cgagtttgga ggcggcgtag gggctgagtg
     1921 gccgggtggc gaggtcttcg tgtttgggca gttcggggtt ggagccgtac accgaggagg
     1981 acgaggcgac gatgacctgc acgtcgccgg cgcggcgggc ggcttcgagc acgttgaggg
     2041 tgccggtggc gtttgcgtga tgggaggcaa ccgggtcttt caccgagcgg ggcaccgacg
     2101 ggcgagcggc gaggtgtacc acggcggagg ccccggcgat agcggcgtcg agttcgtcgg
     2161 ggttcaagat gtcgccctcg accaggcgca cgacggaggt gtcgaggccg tcgaggttgg
     2221 tgcggaaccc ggtggagaga ttgtcgaagg cgacgacctc ggtgatctcg tcggtgttcg
     2281 acatgatccg ggcgagattc gccccgatga atccggcacc gccggtgatg acaaccttca
     2341 cgtgttctcc gatgatcgtg ttggcgggcg tggtggtcag gggcgaaggt gcgccgctct
     2401 gctgttcgct acccaccgat tcagcctcgt cagtatgcca gcatgcgccg gaatcgggca
     2461 tgtgggtggt tcacccagtg ccgatgccca gcacgttctc atcgaagtag taccgaagct
     2521 ttccgcgccg cgcagccatt gctatgcggc ctcgtagcgc agggcatctt cgacttcaga
     2581 gaccgtgagg tcgaacgtgt cggctattgc ctcgatctct tcgccggtct tgaactgttc
     2641 gaagataact tcggtcctga ctcctcgcac cgccggagat ccgctgttcc tgtacggatc
     2701 gatggtgacc gacgcagacg tcgccaatgg gcggatcaat acggccgtgt tgtcccgatc
     2761 ggggtcgaat tccgtggtcg aataaaaggt ctgtgctccg gtagacagct tgagctggcc
     2821 actcgcgacg acgacaagac tcagacgatg atcgagcccg acctcctctt gcaccttaag
     2881 cacgagatct cgaccgtcag tgaacggccg agcgtgggcg agcgggtact tcgtttggaa
     2941 tctctgccgg aggagtgcga ccgcgggtcg catctgctgc atcgagactc ctgcattgcg
     3001 gaactccgag aggtatcggg tttcgacgaa ctcaccccag gtgacgtatg ggctgtcggt
     3061 cgggtgctca cggacgaccg gtgcgtactg tttgccgccg cgggtgtagc cgtcgatcca
     3121 ccgctttgag gtgcctgcgc gaagatggag gagccgatcg acttcgccgt acccgtaggt
     3181 tggtcggtcg aggagatcca cggtgttggg catggcctca tcgtaacggc gaggcctgac
     3241 actcacgccg gtgatgcgta acgtcgcggt gtggtcgttg gggtggtgag tggtctcgat
     3301 gctgcgttcg tgcagcggtg gcatctcgaa ctcgcggcgg ccgggctggt gctcggggtt
     3361 gtggtcgcgc tggcgatgtg gctgagccgc agcgtgatgc ctcgggcgtg tggcgacctc
     3421 gaggtgtggc tgccggtgtt gttcaccctg acccttgtcg gcctgtgggc gacggttccc
     3481 gacacggagc
//
LOCUS       DAOOMA010000251         3488 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_32658_length_3488_cov_4.106904, whole genome shotgun sequence.
ACCESSION   DAOOMA010000251 DAOOMA010000000
VERSION     DAOOMA010000251.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 3488)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3488)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3488
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32658_length_3488_cov_4.106904"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..635
                     /locus_tag="PLP95_07590"
     CDS             <1..635
                     /locus_tag="PLP95_07590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020378495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lipid-transfer protein"
                     /protein_id="HPB45697.1"
                     /translation="GLQARAMLDAGKITEAEMAQIAFDSREAAKANPNAQLQGSAPVE
                     ELLAAPRIADPLRRHDVAPITDGGCAIVLAAGDAVAKWADRPAYIRGIDHRIDSHNFG
                     ARDLTDVPSARLAAERAGVANGPVDVAELHAPFTHQHKMLVEALGLGDDVDVNPSGGA
                     LAANPMMGAGLIRIGEVANRIMSGEATRGVAHATSGHLLQQNLVAVLEGA"
     gene            637..1809
                     /locus_tag="PLP95_07595"
     CDS             637..1809
                     /locus_tag="PLP95_07595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020378494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase domain-containing protein"
                     /protein_id="HPB45698.1"
                     /translation="MANRAAVIGIGQTKYSAVRGDVSLAGLLREAVFRALDDAQLTMD
                     DIDAVVVGKAPDFFEGIMMPEGYLAEALGAVGKPLFRVHTAGSVGGSTAIVAASHVEA
                     GAAERVLTIAWEQQSCSEAMWALSFPIPFQQQLVAGAGGYFAPFIREYMRRSNAPEDI
                     GILVALKDRLNALKNPYAHLHEPDITYDSVRDSFMLWEPIRYSETCPSSDGAIAMVIA
                     GEAAAEASAAATGTNPAWIHGTAMRSEPAMANGRDQVSPQAGQDCATEVYRQAGIANP
                     IEEIDVVEMYVPFSWYEPMWLENLHFAAEGDGWKLTEAGETQMTGKLPVNCSGGVLST
                     NPIGASGMIRFGEAAMQVRGAAGEHQIDGAKRALGHAYGGAAQFYAMWIVGSDKPE"
     gene            complement(1908..2951)
                     /locus_tag="PLP95_07600"
     CDS             complement(1908..2951)
                     /locus_tag="PLP95_07600"
                     /inference="COORDINATES: protein motif:HMM:NF039286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HPB45699.1"
                     /translation="MARSPFPVPYGWFCIGWSEDVAVGETKPLQYFGQHIVLWRDEEG
                     ASHVQDAFCPHLGAHLGHGGKVLGCEIACPFHGWRFDEDGNNTLIPYSSRTNAKGKVK
                     TYPSVEVNGLIFAWYHPDHVEPMWEVPVVPEFNDPDNFTEPVHRIFQVAAPWQELAEN
                     GVDAAHFRYVHNTEEVPELVEYTTTGPIANMKSKQLFPTPRGVVEGYINSDSFGPGLS
                     VVHFGGIIDTVLMGTNVPVDENHCEMRFTFTVRKLGDDAALNSSVGAAFVDEVTRQVR
                     EDKPIWENKAFVPRPALADTDGPFTKFRRWASQFYTTPVEAPLDFYPPDGSYPAEELG
                     KSASQKYGSAPIA"
     gene            complement(3101..>3488)
                     /locus_tag="PLP95_07605"
     CDS             complement(3101..>3488)
                     /locus_tag="PLP95_07605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HPB45700.1"
                     /translation="CCVGVPGPRVPMEPQKVEYIDEVCWFFPELKFVMRHGAEPWVDL
                     AVKLMLKWPNLYYSTSAFRPKHYPKAIIDYANTRGADKIMYAGYFPMGLSLERIFGDL
                     PDVAFNDDVWPKFLRHNAARLFGLND"
BASE COUNT          623 a         1077 c         1162 g          626 t
ORIGIN      
        1 ccggcctgca ggcccgtgcg atgctcgacg ccggcaagat caccgaggcg gagatggccc
       61 agatcgcctt cgacagccgc gaggcggcca aggcgaaccc gaacgcccaa cttcaaggtt
      121 cggctccggt cgaggagctg ctggcggcgc cgcgcatcgc cgatccactg cgccgccacg
      181 acgtcgcgcc gatcaccgac ggcggctgtg cgatcgtgct ggccgccggg gatgccgtcg
      241 cgaagtgggc cgatcgtccg gcctacatcc gtggcatcga tcaccgcatc gacagccaca
      301 acttcggtgc ccgcgacctg accgatgttc cgtcggcacg cctcgccgcc gaacgcgccg
      361 gagtcgccaa cggcccagtc gatgtggccg aactccatgc cccgttcacc caccagcaca
      421 agatgctggt cgaggccctc ggccttggcg acgacgtcga cgtgaacccc tcaggtggtg
      481 cgctcgccgc caacccgatg atgggtgcgg gtctcattcg tatcggagag gtcgccaacc
      541 ggatcatgtc cggtgaagcg acccgcggcg tcgcccatgc gacgagtgga cacctgctgc
      601 aacagaacct cgtggcagtc ctggaaggag cctgacatgg cgaatcgtgc agcagtaatc
      661 ggaatcggtc agaccaagta ctccgccgtg cggggcgacg tgtcgctcgc cggtcttctt
      721 cgcgaggccg tctttcgagc actggacgac gctcaactca cgatggatga catcgacgca
      781 gttgtggtcg gcaaggcccc agatttcttc gagggcatca tgatgcccga ggggtatctc
      841 gccgaggcgc tcggagcggt cggcaaaccg ctgtttcggg tccataccgc tggctcggtg
      901 ggcggctcga ccgcgatcgt cgcggccagc cacgtcgagg ccggcgccgc cgagcgggtg
      961 ttgaccatcg cgtgggaaca gcagtcgtgt tccgaggcga tgtgggcgtt gtcgttccct
     1021 atcccgttcc agcagcagtt ggtggcgggc gcgggcggct acttcgcccc gttcatccgc
     1081 gagtacatgc gccgttcgaa tgctcccgag gacatcggca tcctcgtggc gttgaaggac
     1141 cgtctgaacg cgttgaagaa tccgtatgcc cacctgcatg agcccgacat cacctatgac
     1201 tcggtgcgcg actcgttcat gttgtgggag ccgatccgct actccgaaac ctgcccgtcc
     1261 tccgacggag cgatcgccat ggtgattgct ggtgaggccg cagccgaggc ctccgctgct
     1321 gccactggaa cgaacccggc atggatccac ggcacagcga tgcgctcaga gccggcgatg
     1381 gccaacggac gagatcaggt ctcgccacaa gcgggccagg actgtgcgac cgaggtgtat
     1441 cgccaagcgg ggatcgccaa cccgatcgag gagatcgacg tcgtcgaaat gtacgtgccg
     1501 ttctcctggt acgaaccgat gtggctcgag aacctgcact tcgccgccga gggcgacggc
     1561 tggaagctca ccgaggccgg cgagacccag atgaccggaa agctgccggt caactgctcg
     1621 ggtggcgtgt tgtcgacaaa cccgatcggg gcgtcgggca tgattcgctt cggcgaggcc
     1681 gctatgcagg tgcgcggtgc cgctggcgag catcagatcg acggcgccaa gcgtgcgctc
     1741 ggccatgcct acggcggtgc ggcgcagttc tacgccatgt ggatcgtcgg atccgacaag
     1801 ccggagtagg aggtagccat tccccccaaa agccgttctc cgataacgag tggtcgtgat
     1861 cacgaccact cgttatcgga gaacgggctt tcgcgtgtgc gagagggtca ggcgatgggt
     1921 gcggagccgt acttctggct ggcggacttg cccaactctt ccgccgggta gctgccgtcg
     1981 ggcggataga agtcgagtgg ggcttcaacc ggggtcgtat agaactgcga agcccaccgg
     2041 cgaaatttgg tgaaggggcc gtcggtgtcg gcgagggcgg gacggggcac gaacgccttg
     2101 ttttcccaga tcggcttgtc ctctcggacc tgccgcgtca cctcatctac gaaagccgca
     2161 ccgacactgg agttgagcgc agcgtcgtcg ccgagcttgc gaaccgtgaa ggtgaagcgc
     2221 atctcgcagt ggttctcatc tacgggaacg ttggtgccca tcaagacggt gtcgatgatg
     2281 ccgccgaaat gcacgaccga aagtccggga ccgaaggagt cggagttgat gtagccctcc
     2341 acgacacctc ggggggtggg gaagagctgc ttggacttca tgttggcgat cggcccggtg
     2401 gtcgtgtatt cgacgagttc gggcacctct tcggtgttgt ggacgtatcg gaagtgcgct
     2461 gcgtcgaccc cgttttcggc gagttcctgc cacggtgctg cgacctggaa gatccgatgc
     2521 accggctcgg tgaagttgtc ggggtcgttg aattcgggaa cgacgggaac ctcccacatc
     2581 ggctcgacat ggtcagggtg gtaccacgcg aagatcagtc cgttgacttc aaccgagggg
     2641 taggtcttga ccttgccctt ggcgttggtg cgcgagctgt aggggatgag ggtgttgttg
     2701 ccgtcctcgt cgaaacgcca accgtggaag gggcatgcga tctcacagcc gagcaccttg
     2761 ccgccatgcc caaggtgtgc acccaggtgc gggcagaagg cgtcttggac gtgtgaggca
     2821 ccctcttcgt cgcgccacaa cacgatgtgt tggccgaagt actgcagcgg cttggtttcg
     2881 ccgactgcga cgtcttcaga ccatccgatg cagaaccagc cgtacggtac cgggaagggt
     2941 gagcgcgcca taatgagccc ccgttgggtc gttcgaggcc tggcagccac gaaaaaacta
     3001 gaacgtgttc tagtatatcg atcgctgatg gtgggtcaac cgggtcggag gtcccggacc
     3061 gaccgccggt ccgggacagc gcgaacggtt cggggcagcg ctagtcgttc agcccgaaca
     3121 gccgcgccgc gttgtggcgc aggaacttgg gccagacatc gtcgttgaac gccacatcgg
     3181 gcaggtcgcc gaaaatgcgc tcgaggctca gtcccatcgg gaagtaccct gcgtacatga
     3241 tcttgtcggc gccccgcgtg ttcgcgtagt cgatgatggc cttcgggtaa tgcttcggcc
     3301 gaaacgctga ggtcgagtag tacaggttcg gccacttcag catcaacttg acggccaggt
     3361 cgacccacgg ctcggccccg tgacgcatca cgaacttcag ctcggggaag aaccagcaca
     3421 cctcgtcgat gtactcgacc ttctgcggtt ccatcggcac gcggggtccg gggaccccga
     3481 cgcagcac
//
LOCUS       DAOOMA010000252         3471 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_32854_length_3471_cov_5.155445, whole genome shotgun sequence.
ACCESSION   DAOOMA010000252 DAOOMA010000000
VERSION     DAOOMA010000252.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 3471)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3471)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3471
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32854_length_3471_cov_5.155445"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..206)
                     /locus_tag="PLP95_07610"
     CDS             complement(<1..206)
                     /locus_tag="PLP95_07610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB45701.1"
                     /translation="MTSVNPKSRTWVKGSPRRPNKTQIVALTVVSLLCLGPVVALIVA
                     MNRPSNPLHLLSEETEIPNDGARVR"
     gene            complement(278..1918)
                     /locus_tag="PLP95_07615"
     CDS             complement(278..1918)
                     /locus_tag="PLP95_07615"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HPB45702.1"
                     /translation="MMCTKRLVVVISALALSASACSSATADEGPDQRPAGASNTTSLE
                     QAAGIGPAGSGPAPATVTLGLLLDVDSFDPLGSLGDSGALQAFRFVYDTMVRVDRDGK
                     VVPGIAESWTTEGTSTTFQIRSGLTCSDGTALDAAAVGASIRTLGTEGGMGGAGVFGP
                     GGLKEVRVDEGANTVTIETGALYNEMLPAMTSSSHIVCPAGLVDREALRRTPQGSGPY
                     ELISSQPGTYLLKRRDAYTNLPAGTAITDLPQTVTLSVIGDDATAADLLAQNRVQIIA
                     TQSSISSFLASDPTLVAVPAAGWGTNAVTFMQREQSATADPQLRRGMAMLLDSVEGSA
                     AETGGTGISRRTMFTPNLACYSEDLAKAAPAYDPAAAAALLDSAGYVVGADGFRTRPD
                     GTPLVIRVVGNNFQGMAPQYIADALEAGGFHVDLFVGTYDESIAKLLSDNFDVGMYPY
                     VSSDPSLSSWPNQIGTSAVANFSRISNLEFDAYAAAALAADVDSEQRCSSWHEAERAA
                     LSAADIVPFSQPVKHWFGNGVTFDATYFTVDPFSIRTA"
     gene            2139..3158
                     /locus_tag="PLP95_07620"
     CDS             2139..3158
                     /locus_tag="PLP95_07620"
                     /inference="COORDINATES: protein motif:HMM:NF018447.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase family protein"
                     /protein_id="HPB45703.1"
                     /translation="MPSTMVTRSKRWIAALSLAVAGSLGVTGGLNLPGAGEFPVPMTL
                     PEAAAQSTAPLPSLSDAQAAVVAAEAQVDAVARMQTAAQHRLEGLNLSEASLVEQIAA
                     ARREMRRWTVDAYTSDRHGLAQLLAEQSDPMERSARLTFASSTASQFDEAVRHYERLK
                     AQVEPALVELADGIDQLAADRQSADNALLAARAMEAEAERLHAEAQRQAAAAKALKTR
                     ATQAPTHAQPNTNADADTGAGASSSDRPGDGAVQGAPAPAGGPTEEQWERLRHCEATG
                     NYQAVSASGKYRGAYQFDYQTWATLGPPGDPAAASPAEQDRRARLLYASRGWRPWPQC
                     GRHLR"
     gene            complement(3166..>3471)
                     /locus_tag="PLP95_07625"
     CDS             complement(3166..>3471)
                     /locus_tag="PLP95_07625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB45704.1"
                     /translation="LLAAIVAHWPSVGPDAFDLLARPRRLSELEVEQLGRVVDGRLRT
                     RSTGVLLDDGRQIRRAPSGRWAIVATRRSGRQVIEAIGEDLVELLDGAEVDLRDPDR"
BASE COUNT          579 a         1231 c         1124 g          537 t
ORIGIN      
        1 cgcactcgcg ctccgtcgtt cggaatctcg gtctcctcgg acaacagatg cagcgggttc
       61 gacggccgat tcatcgcgac gatgagggcg accaccggcc ccagacacaa cagcgacacc
      121 accgtcagtg caacgatctg ggtcttgttc gggcgccgag gcgacccctt cacccacgtt
      181 cgactcttgg ggttcaccga cgtcacccga acatcctccc aaacccgacg cccgccgcgg
      241 gtgccatggc ctgtgacggg cggccgaccg ccgtgatcta cgccgttcgg atcgagaagg
      301 gatcgacggt gaagtaggtg gcgtcgaagg tcacgccgtt gccgaaccag tgcttcaccg
      361 gctgcgagaa cggcacgata tcggccgccg acagcgcagc gcgctcggct tcgtgccacg
      421 aggagcatcg ctgctctgag tcaacgtctg cagccagcgc cgccgccgca tacgcgtcga
      481 attccaggtt gctgatccgc gagaaattcg cgacggcgga ggtgccgatc tggttcggcc
      541 acgaactcag ggaggggtcc gaggagacgt acgggtacat gccgacgtcg aagttgtcgc
      601 tcagcagctt ggcgatcgat tcgtcgtagg tgcccacaaa cagatcgaca tggaagccgc
      661 ccgcttcgag cgcgtcggcg atgtactgcg gggccatccc ctggaagttg tttccgacga
      721 ctcggatgac gagcggggtc ccgtccgggc gcgtccgaaa cccgtcggcg ccgacgacgt
      781 agcccgccga atcgagcagc gcggccgccg cagccgggtc ataggccggt gcggccttgg
      841 ccaggtcctc gctgtagcac gcaaggttgg gggtgaacat cgtccggcgg ctgatgccgg
      901 tgcctcccgt ctcggcggcc gacccctcga cgctgtcgag caacatggcc atccctcgcc
      961 gcagctgcgg gtccgcggtg gccgactgct cacgctgcat gaaggtcacc gcattggtcc
     1021 cccatcctgc tgctgggacg gcgaccaggg tcgggtcgct cgccaggaag ctcgagatcg
     1081 acgactgcgt cgcgatgatc tgcacgcggt tctgagcgag caggtccgcc gccgtcgcgt
     1141 catcgccgat caccgacagc gtcacggtct gaggcaggtc ggtgatggcg gttccggcgg
     1201 gcagattggt gtaggcatcg cggcgcttca acaggtaggt gccgggctgc gaactgatca
     1261 gttcgtaggg gcccgaacct tgcggggtgc gccgcagggc ctcccgatcg accaaacctg
     1321 ctgggcacac gatatggctg ctgctggtca tcgccgggag catttcgttg tacaaggcac
     1381 cggtctcgat cgtcacggtg ttcgccccct cgtcgacacg gacctccttg agtccgccgg
     1441 ggccgaacac ccctgctcca cccattccgc cctcggtacc gagggttcga atggacgcac
     1501 cgaccgcagc ggcatcgagc gccgttccat cggagcacgt cagccccgag cggatctgga
     1561 aggtggtcga ggtcccctcg gtggtccacg actcggcgat tccgggaacc acctttccgt
     1621 cgcggtccac ccgcaccatc gtgtcgtaga cgaaccggaa tgcctgaagt gcgccagagt
     1681 ctccgagcga tccgagcggg tcgaatgaat cgacgtcgag cagcagcccc agggtgaccg
     1741 tcgccggagc cggcccggac ccggcgggac cgattcccgc tgcctgttcc agcgacgtgg
     1801 tgttcgaggc accggccggc cgctggtcgg gaccttcatc cgctgtcgcg ctcgaacagg
     1861 ccgaggccga cagcgccagc gcggaaatga cgacgacgag tcgtttcgtg cacatcattc
     1921 gggccacggt aggagcgaaa ttctccgttg cggttgggcc gttcggctca tttgcgcgag
     1981 gcggccttga ccggccccgt tcaccgccga tcactcccat cgatttctca tcgtcaccct
     2041 cggcgcgcga atgtctcgct gttcgggttc ccgttcgcta cgcccgagta tcaggtgatc
     2101 gcttcaatgt ctgtcgaaac catgccgatc ggtgacacat gcccagcacc atggtgacgc
     2161 gctcgaagcg gtggatcgct gcgctctcgc tcgccgtcgc cggttccctc ggcgtcaccg
     2221 gcgggctcaa cctccccggc gccggcgagt tcccggtgcc gatgaccctc cccgaggcgg
     2281 cagcccagag caccgcgcca ttgccttcgc tcagcgacgc ccaagccgcc gtcgtggcag
     2341 ccgaggccca ggtcgatgcg gtcgctcgca tgcagaccgc cgcgcagcat cgactcgaag
     2401 gtctgaacct cagcgaggcc tcgctcgtcg aacagattgc ggccgcccgc cgggaaatgc
     2461 gccgctggac cgtcgatgcc tacacctcgg accgccacgg cctcgcgcag ttgctggcag
     2521 aacaatccga cccgatggag cgatccgccc gcctcacgtt cgcctcgagt acggccagcc
     2581 aatttgacga ggcggtgcgt cactacgaac ggctcaaagc tcaggtcgag ccggcgctcg
     2641 tcgaactcgc ggacgggatc gaccaactcg cagcagaccg ccagtcggcc gacaacgccc
     2701 tccttgccgc acgagcgatg gaggccgagg ccgaacggct tcacgccgag gcccaacgcc
     2761 aggcggcggc cgccaaggcg ctcaaaaccc gagcgaccca ggcgccgacc cacgcccaac
     2821 ccaacaccaa tgccgacgcc gacaccggcg ctggcgcctc gagcagcgat agacccggcg
     2881 acggtgccgt ccagggcgct cccgctccgg ccggcggacc gaccgaggag cagtgggaac
     2941 gcctgcgcca ttgtgaagcg accggcaact accaggccgt gtccgcatcg ggaaagtacc
     3001 gtggcgccta ccagttcgac taccagacct gggcgaccct cggacctccc ggcgatccgg
     3061 cggcagcatc gccggccgaa caagaccgtc gagcgcgctt gctgtatgcg tcgaggggtt
     3121 ggcgtccctg gcctcagtgc ggccgacacc tgcgctgagc cgctctcatc gatcgggatc
     3181 gcgaaggtcg acctcggcgc cgtcgagcag ttcgacgaga tcctcgccga tcgcctcgat
     3241 gacctgacgg ccgctacggc gggtggccac gatcgcccac cgtccgctcg gcgcccgtcg
     3301 gatctggcgc ccgtcgtcga gcagcacccc cgtcgaacgg gtccgcaacc ggccgtcgac
     3361 gacacgaccg agctgctcga cctccaactc gctcaaccgt cgggggcgcg ccaagaggtc
     3421 gaatgcgtcg gggccgacgc tcggccagtg ggcgacgatc gccgccaaca g
//
LOCUS       DAOOMA010000253         3465 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_32941_length_3465_cov_4.132551, whole genome shotgun sequence.
ACCESSION   DAOOMA010000253 DAOOMA010000000
VERSION     DAOOMA010000253.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 3465)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3465)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3465
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32941_length_3465_cov_4.132551"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            99..1403
                     /locus_tag="PLP95_07630"
     CDS             99..1403
                     /locus_tag="PLP95_07630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012226332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HPB45705.1"
                     /translation="MEHRGDAISTPRPPSGGSRPQRADLVVHPPVPPADLDEFRSAAL
                     AFLDAHATPKVADGPFEWGVGDDFVGIVEEPDPETEALAIAAAKEWAATRWNAGFGWL
                     TGPVDQGGAGLTGEHLRIYRGLESGYEVADQSIFMIALGIVGPTIAAHARPEIAAQYL
                     APIHRGELICCQLFSEPEAGSDLANVQARAVRDGDDWLITGQKVWTSNAQHAQIGEII
                     CRHDPDAPKHAGLTAFLVDMTAPGVEVRPLRQMTGGAGFNEVFFTEVRVPDSHRLGEV
                     GEGWKVATTTLMNERSSIGSGMGLGRGPGPFERLIELTRVMGKADDPIVRDKLMKLYI
                     GNRVASYTGKRGAAAIKAGRLPGPELSVLKLANTLNLLATADYAAEVLGPKLCPDTGE
                     WGTFAWTKLVGSAPGGRLGGGTDEIQRNIIGERVLGLPREPK"
     gene            complement(1360..2352)
                     /gene="rarD"
                     /locus_tag="PLP95_07635"
     CDS             complement(1360..2352)
                     /gene="rarD"
                     /locus_tag="PLP95_07635"
                     /inference="COORDINATES: protein motif:HMM:TIGR00688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter RarD"
                     /protein_id="HPB45706.1"
                     /translation="MSAPEDERPKSQSGSPTSDERIGLTAGVAAYVIWGAFPIFFHSL
                     EPTSALEILAHRIVWTGVFLAIVLQITGRWGWVGELRADPAKLRRAWLAGLLISINWL
                     TYVWAVGADRVLDASLGYFINPLLSVVLGVALLGEGIRRLQVLALTIGAVAVVVLSVG
                     YGEFPIVGIVLALSFGFYGYLKKGMELDGLASLAAETSIVWPIAVIGLAVAFATGSST
                     FGHDNVGLNLRLLAAGPITAVPLALFGVAAPRLTLVTVGILQYITPMAQFFLGWWVFH
                     EEMPPERLAGFALVWLALVILTADAVATIRRNRSVRPDAAATSARGEGRAPARR"
     gene            complement(2349..3278)
                     /locus_tag="PLP95_07640"
     CDS             complement(2349..3278)
                     /locus_tag="PLP95_07640"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03564 family F420-dependent LLM class
                     oxidoreductase"
                     /protein_id="HPB45707.1"
                     /translation="MELGIFDAVASNAGTIDALVASAAATHDDGFASWWLPQIFGFES
                     LSVHAIVGREVPELALGTAVVPTYPRHPVTMAQLALTAQSAAAGATGANRLTLGIGLS
                     HQMVIENMFGLSFDRPAHHMSEYLDVLMPLLETHSVSAHGETISTSVGLTVPDGIVAP
                     DVVVAALGPAMLRIAGTKTQGTVTWMTGPATLESHIVPSIRAAATEAGRPAPRVVAAL
                     PVLVTDDEAAGRATVGSVFEIYGFLPSYRAMLDREGVEGPADVAIVGTEDEVASKIRA
                     LDGIGVTDFVAVEFATPGSRDAQRTRELLRSLL"
     gene            3277..>3465
                     /locus_tag="PLP95_07645"
     CDS             3277..>3465
                     /locus_tag="PLP95_07645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB45708.1"
                     /translation="MRSTYPAPTPPGRTIPAGADQLRSGGSGPGGVVAIFAVRDGGLR
                     SLGVAGVLGVGLAGWLSGV"
BASE COUNT          598 a         1099 c         1235 g          533 t
ORIGIN      
        1 ccgtttcccg ataaccagtg gtcgtgatca cgaccactgg ttatcgggaa acgggtcaag
       61 ttattggcgg cgcgtcaata gtgtgcaaga ctgcgcacat ggaacaccgg ggggatgcca
      121 tctcgacgcc gcggccaccg tctgggggtt cgcgcccgca gcgggccgac ctcgtggtgc
      181 accctccggt tccccccgcc gatctcgacg agttccgatc cgcggcgttg gcgtttctcg
      241 acgcccacgc gacgccgaag gtggccgacg gtcccttcga gtggggggtc ggcgacgact
      301 tcgtcggcat cgtcgaggag cccgaccccg aaaccgaggc cctcgccatc gccgcggcca
      361 aggagtgggc ggcgacccga tggaacgccg ggttcggctg gttgacgggc cccgtcgatc
      421 agggtggagc cggccttacc ggcgagcacc tgcggatcta ccgcgggctc gaatcgggct
      481 atgaggttgc cgatcagtcg atcttcatga tcgccctcgg catcgtcggg ccgacgatcg
      541 ccgcgcatgc ccgcccggag atcgccgcgc agtacctggc gcccatccat cgcggcgaac
      601 tcatctgctg tcagcttttc agcgagcccg aggcgggctc ggacctggcg aacgtgcagg
      661 cccgcgcggt gcgcgacggc gacgactggc tgatcaccgg acagaaggtg tggacctcca
      721 acgctcagca cgcgcagatc ggcgagatca tctgtcgcca cgaccccgat gccccgaaac
      781 acgcggggct cacggccttc ctcgtcgaca tgaccgcacc cggtgtcgag gttcgtccgc
      841 tgcgtcagat gaccggggga gcggggttca acgaggtgtt cttcaccgag gtgcgggtgc
      901 ccgacagcca ccgtctgggc gaggtgggcg aaggctggaa ggtggctacg accacgctga
      961 tgaacgagcg cagctcgatc ggttcgggca tgggactggg ccgcggcccg ggtccgttcg
     1021 aacgactcat cgagttgacg cgggtgatgg gcaaggcgga tgacccgatc gttcgcgaca
     1081 agttgatgaa gctctacatc ggcaatcgcg tcgcgtccta cacgggcaaa cgcggcgcgg
     1141 cggcgatcaa ggccggtcgc ctgcccggcc ctgagctcag cgtgttgaag ctggcgaaca
     1201 ccctcaacct gttggccacc gccgactatg cggccgaggt gctcggtccg aagctgtgcc
     1261 cggatacggg ggagtggggc acctttgcgt ggaccaagtt ggtggggagc gctccgggcg
     1321 gtcgcctcgg aggcggcacc gacgagattc agcgcaacat catcggcgag cgggtgctcg
     1381 gccttccccg cgagccgaag tagctgcggc gtcgggtcgg acgctgcggt tgcggcggat
     1441 cgtcgcgacg gcgtctgcgg tgaggatgac gagggcgagc cacacgagcg cgaatccggc
     1501 gaggcgctcg ggcggcattt cctcgtggaa cacccaccag ccgaggaaaa actgcgccat
     1561 cggggtgatg tactgcaaga tgccgacggt cacgagtgtg agccgtggtg ccgccacgcc
     1621 gaagagggcg agggggaccg cggtgatcgg tccggcggcg aggagtcgga ggttgagccc
     1681 gacgttgtcg tgtccgaacg tggacgaccc ggtcgcgaac gcgacggcca ggccgatgac
     1741 cgcgatcggc cacacgatgg aggtctccgc cgccagggat gcgagcccgt cgagttccat
     1801 gcccttcttg aggtagccgt agaacccgaa tgacagcgcc aacacgatgc cgacgatcgg
     1861 gaattcgccg tagccgacgc tcaagacgac caccgctacc gctccgatcg tgagggcgag
     1921 gacctggaga cgccggatgc cctcgccgag caacgccacg ccgagaacca ccgacagcag
     1981 cgggttgatg aagtagccga gcgaagcgtc gagtacacga tcagcaccga ccgcccagac
     2041 gtaggtgagc cagttgatgg agatcagcag tccagcaagc catgcccgac gtagcttcgc
     2101 tgggtcggcg cgaagttcgc cgacccaccc ccaccgcccg gtgatctgca acacgatggc
     2161 gagaaacacc ccggtccaca cgatgcgatg ggcgaggatc tcgagcgccg aggtcggttc
     2221 gagcgaatgg aagaagatcg gaaacgcacc ccagatcaca tacgcagcga cgcccgcagt
     2281 gagaccgatt cgttcatcgc ttgtcggcga tccgctctgg gacttcgggc gttcgtcttc
     2341 cggtgcgctc acagcagtga gcgcagcagt tcgcgggtgc gttgagcgtc gcgagaaccg
     2401 ggggtggcga actcgacggc gacgaaatcg gtgacgccga tgccgtcgag cgcacggatc
     2461 ttcgatgcca cctcatcctc ggtgccgacg atggcgacgt cggccgggcc ttcaacacct
     2521 tcgcggtcga gcatcgcgcg atagctcggc aggaacccgt agatctcaaa caccgacccg
     2581 accgtggcgc ggcctgccgc ctcgtcgtcg gtgaccaaca ccggaagcgc cgcgacgact
     2641 cggggtgcgg ggcggccggc ctcggtggcg gcagcgcgga tcgacggaac gatgtgggac
     2701 tcgagggtgg cggggccggt catccaggtg accgttccct gcgtcttcgt cccggcgatt
     2761 cgcaacatgg cggggccgag cgcggcaacg acgacatcgg gcgcgacgat tccgtcgggc
     2821 accgtgaggc cgaccgaggt ggagatcgtt tcaccgtggg ccgataccga atgggtctcc
     2881 aacagcggca tgaggacatc gaggtattcc gacatgtggt gggccggccg gtcgaacgac
     2941 aggccgaaca tgttttcgat gaccatctga tggctgagcc cgatgccgag cgtgagccga
     3001 ttggcaccgg tggccccggc cgcggccgac tgggcggtga gcgccagttg cgccatcgtg
     3061 accggatggc gcgggtaggt gggaaccacg gcggtgccga gtgccagctc cggcacctcg
     3121 cgaccgacga tggcgtgcac cgacaacgac tcgaacccga agatctgcgg cagccaccac
     3181 gatgcgaagc cgtcgtcgtg ggtggcggcg gctgaggcga cgagcgcatc gatcgtcccc
     3241 gcgttactgg caacggcgtc gaagattccg agttccatgc gctcaaccta cccggccccg
     3301 acgccgcccg gccgaacgat tccggctgga gccgatcagc tccgatctgg aggaagcggc
     3361 cccggcggtg tcgttgcgat cttcgccgtg cgcgacgggg gactgcggtc tctgggggtt
     3421 gcgggggtgt tgggtgttgg gcttgcgggt tggttgagtg gggtt
//
LOCUS       DAOOMA010000254         3457 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_33041_length_3457_cov_4.370370, whole genome shotgun sequence.
ACCESSION   DAOOMA010000254 DAOOMA010000000
VERSION     DAOOMA010000254.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 3457)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3457)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3457
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33041_length_3457_cov_4.370370"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..194
                     /locus_tag="PLP95_07650"
     CDS             <1..194
                     /locus_tag="PLP95_07650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020379077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB45709.1"
                     /translation="ADGAYIRSAAGRNGVPLFTTAAAALAAAEGIADTVSMPLVVRSL
                     QEYHAGAVTSDQAHPESGS"
     gene            191..1117
                     /locus_tag="PLP95_07655"
     CDS             191..1117
                     /locus_tag="PLP95_07655"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020379078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HPB45710.1"
                     /translation="MSVDLSVEVGSLRLPNPIMVASGTAGHSTELSAYLDLSSLGAFV
                     VKSLSAQPWAGNPGPRVHQSPLGMINSVGLQGPGIEAWLAEGLPALRRAGVERIVVSI
                     WGRSVEDYPRAAAMLADAPEEVIAVEVNLSCPNLEGGKHLFAHDPVLSATVIERCAVA
                     GRPLWAKLSPNTDRLVEVAGAVAGAGAESVTLVNTLLGMSIDVETRRPRLGSGAAGGG
                     VSGPAIHPVAVRAVYDCRVAHPDLPIVGVGGIATEVDAVEFLLAGAQACQVGTSTFAD
                     PRTPGNVLRGIEDWCARHSISEVRELSGGAHD"
     gene            1110..1817
                     /gene="pyrF"
                     /locus_tag="PLP95_07660"
     CDS             1110..1817
                     /gene="pyrF"
                     /locus_tag="PLP95_07660"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF012441.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HPB45711.1"
                     /translation="MTDRTTETPDEVRRKLCLVIDTDDLVEARRLATSMRPWFGVAKV
                     GLELFSAAGPDVIADLRDLGYRVFLDLKLYDIPTTVNRASRVLGALGVDYLTLHATGG
                     LDMLKAGVEGLYSGAENAGLDAPVALAVTVLTSDDSAPPHIVPNRVRLALEGGCGGLI
                     LAATDLQTARELAPRLRRVVPGIRPAGAPVHDQARAATPQDAIANGADLLVIGRAVTQ
                     AEDPLEAAAQIAASISV"
     gene            1814..2206
                     /locus_tag="PLP95_07665"
     CDS             1814..2206
                     /locus_tag="PLP95_07665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB45712.1"
                     /translation="MNDRIAGGADVGSAGSAGSAALAGSAATASSIRAERDAVLCDVA
                     LSGFAIADLERLAAERDGWAVQTLRLLPCLEAMGAIGGKVRSRRAIAAAGLEEDVALG
                     ALSDAQWRALAQAVEANEAAQANEEEAQ"
     gene            2203..2757
                     /locus_tag="PLP95_07670"
     CDS             2203..2757
                     /locus_tag="PLP95_07670"
                     /inference="COORDINATES: protein motif:HMM:NF012832.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB45713.1"
                     /translation="MIIVVSGPGGVGKGTVVNEWLRRNPRLYVNRSWTTRAVRPGEAH
                     DAYVFATPEEFQAHIDRDGFLEWVDFLDYRQGSPIPTPPDGHDVLFEIDVQGARRILE
                     RYPDALLIWLDAPSRQEQRRRLIGRGDTAEHVERRIAHAETESAEAAKLGMVHVVNHE
                     VDDTVQQLIEVVEDHRSKGEQQGR"
     gene            2840..3184
                     /gene="rpoZ"
                     /locus_tag="PLP95_07675"
     CDS             2840..3184
                     /gene="rpoZ"
                     /locus_tag="PLP95_07675"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HPB45714.1"
                     /translation="MSAHFDTMMEPRVEDLLEAAGSKFSLVALAAQRARQINTYYTTL
                     GEGLGHIVPPQVTSTASKSLSIAFEEIAAGAIVGVKRSEIEAAEAQAAADAAALDAEA
                     ADAEAADAGADD"
     gene            3224..>3457
                     /locus_tag="PLP95_07680"
     CDS             3224..>3457
                     /locus_tag="PLP95_07680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavoprotein"
                     /protein_id="HPB45715.1"
                     /translation="MLENKRIVVGVTGGIAAYKAVEVIRRLVDAGAHVSPIMTEGARH
                     FIGETTLSALGSEKVQTSLWDEDSVIPHTRLGQG"
BASE COUNT          538 a         1182 c         1208 g          529 t
ORIGIN      
        1 gggccgacgg cgcttacatc cgttcggctg cgggacgcaa cggggtgccg ctgttcacca
       61 ccgccgccgc cgcgctcgcc gccgcagagg gaatcgccga cacggtgtcg atgccgctgg
      121 tcgttcgcag cttgcaggag taccacgccg gagccgtcac ctccgaccag gctcacccgg
      181 agtcgggatc ttgagcgtcg acctgtcggt cgaggtcggg tcgcttcggc tgcccaaccc
      241 gatcatggtc gcgtcgggca cggcggggca ctcgaccgag ctgagcgcct atctcgacct
      301 gtcgagcctc ggcgcgttcg tcgtgaagtc gctgagcgcg cagccctggg cgggcaatcc
      361 gggcccgcgg gtccaccaga gccccctggg catgatcaac agcgtcgggt tgcagggacc
      421 cggcatcgag gcgtggctcg ccgaaggcct tccggcgctt cgccgcgcgg gggtcgaacg
      481 catcgtggtg tcgatctggg gccgcagcgt ggaggactac cccagggccg cagcgatgtt
      541 ggcggatgcc cccgaggagg tgatcgcggt cgaggtcaac ctgagctgtc cgaacctcga
      601 gggcggcaag cacctgttcg cacacgaccc ggtgctgtcg gccacggtca tcgagcggtg
      661 cgcggtggca ggccggccgc tgtgggcgaa actcagcccg aacaccgacc gcctcgtcga
      721 agtcgccgga gcggtggccg gggccggagc ggagtcggtc acgctggtca acaccctgct
      781 cggcatgtcg atcgacgtcg aaacccgacg cccccgcctc ggcagcggtg cggcgggcgg
      841 gggagtgagc gggcccgcca tccatccggt tgcggtgcgg gctgtctacg attgccgggt
      901 cgctcacccc gaccttccga tcgtgggggt cggcggtatc gcgaccgagg tcgatgccgt
      961 cgagtttctg ctcgccggcg cccaggcgtg ccaggtcggc acgtcgacgt tcgctgatcc
     1021 ccgaaccccg ggcaacgtgt tgcgcggcat cgaggactgg tgtgcaagac attcgatttc
     1081 cgaagtacga gagctcagcg gaggcgcaca tgactgaccg gaccaccgaa acccccgacg
     1141 aggttcgacg caaactctgc ctggtgatcg acaccgacga tctggtcgag gcccgccgtc
     1201 tcgcaacgtc gatgcggccg tggttcggcg tcgccaaggt cggcctggag ctgttcagcg
     1261 ccgccggccc cgacgtgatc gcagacctgc gcgacctcgg ctatcgggtc ttcttggacc
     1321 tcaagctcta cgacatcccc accacggtga accgggcgag ccgcgtgctg ggggcgctcg
     1381 gggtcgacta cctcaccctt cacgcgaccg gcgggctcga catgttgaaa gccggggtcg
     1441 aggggctcta cagcggcgcg gaaaacgccg gtctcgatgc ccccgtggcg ctcgcggtga
     1501 ccgtgttgac ctccgacgat tcggccccgc cccacatcgt tcccaaccgc gtgcggttgg
     1561 ccctcgaggg cggatgcggc ggcctcatcc tcgccgcaac cgacctgcag accgcccgcg
     1621 aattggcgcc gcgcctgcgg cgggtggtgc cgggaatccg tcccgccggc gcgccggtgc
     1681 acgaccaagc ccgcgccgcc accccgcagg acgcgatcgc gaacggtgcg gatctgctcg
     1741 tcatcggacg cgcggtgacc caggcggagg acccgttgga agctgcggcg cagatcgccg
     1801 cctcgatctc ggtatgaacg accggatcgc cggcggcgcc gacgtggggt cggccgggtc
     1861 ggctgggtcg gcggctttgg ctgggtcggc tgccacagcg tcgtcgatcc gcgccgagcg
     1921 cgacgccgtg ttgtgcgacg tcgcgctcag cggattcgcc atcgccgacc tggagcgtct
     1981 ggcggccgag cgcgacggct gggcggtgca gacccttcgg ctgctgccgt gcctggaggc
     2041 gatgggcgcc atcggaggca aggtccggtc gcgtcgagcc atcgccgcgg cggggctcga
     2101 agaagatgtg gcgctcggcg cgttgagtga tgcgcagtgg cgagcgctgg cccaggcggt
     2161 ggaagcgaac gaggcagcgc aagcgaacga ggaggaagcg caatgatcat cgtggtgtcg
     2221 ggccccggcg gggtcggcaa ggggacggtc gtcaacgagt ggcttcgacg caatccgcgg
     2281 ctgtacgtca atcgttcgtg gacgacgcgg gcggtgcgac cgggcgaggc tcacgacgcg
     2341 tacgtgttcg ccacccccga ggagttccag gcccacatcg atcgagacgg tttcctcgaa
     2401 tgggtcgact ttctcgatta ccggcagggt tcgccgattc ccacaccacc cgacggccac
     2461 gacgtgttgt tcgaaatcga cgtgcaaggg gcgcgccgaa tcctcgagcg ctaccccgac
     2521 gcgctgttga tctggctcga tgcgccctcg cggcaggaac agcgtcgccg tctcatcggc
     2581 cgcggtgaca ccgccgaaca cgtcgaacgg cgcatcgccc acgccgaaac cgaatccgcc
     2641 gaggccgcga agctcggcat ggttcacgtc gtcaaccacg aggtcgacga caccgtgcaa
     2701 cagctcatcg aggtggtcga agatcaccgt tccaagggtg aacagcaggg tcggtaggcc
     2761 gggctgctag catgacccct cggtgcgcgc gtcggcgcgc cactccgtcg ccctgccgtc
     2821 tgcccgcttg gagaaacgca tgtccgccca tttcgacacc atgatggagc ctcgggtcga
     2881 ggatctcctc gaggccgcag gttcgaagtt ctcactcgtc gcgctcgccg cgcagcgtgc
     2941 ccgtcagatc aacacctact acaccaccct cggcgagggg ctcggccaca tcgttccgcc
     3001 gcaggtgacc tcgaccgctt cgaagtcctt gtcgatcgct ttcgaagaga tcgccgccgg
     3061 cgcgatcgtc ggcgtgaagc gttccgagat cgaggccgct gaggcccagg ccgccgccga
     3121 tgcggccgcg ctcgacgccg aggcagccga cgccgaggca gccgacgccg gcgccgacga
     3181 ctgatcgtcg gggctatcga acagcacgaa cccgaccaca ccgatgctgg agaacaagcg
     3241 catcgtcgtc ggagtcaccg gtggtatcgc cgcatataag gcggtcgagg tcatccgacg
     3301 tctcgtcgat gccggagccc acgtgtctcc aatcatgacc gagggggcgc gccacttcat
     3361 cggtgagacc acgctgtccg cactcggttc ggagaaggtg cagacctcgt tgtgggacga
     3421 ggacagcgtc attccccaca cccggctcgg ccagggc
//
LOCUS       DAOOMA010000255         3439 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_33257_length_3439_cov_3.439716, whole genome shotgun sequence.
ACCESSION   DAOOMA010000255 DAOOMA010000000
VERSION     DAOOMA010000255.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 3439)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3439)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3439
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33257_length_3439_cov_3.439716"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..694
                     /locus_tag="PLP95_07685"
     CDS             <1..694
                     /locus_tag="PLP95_07685"
                     /inference="COORDINATES: protein motif:HMM:NF015254.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="pectin acetylesterase-family hydrolase"
                     /protein_id="HPB45716.1"
                     /translation="DWSMVFVPYCTGDLHLGDAVREDSPEQVVHHSGLANGTAVLDQV
                     EEQFQTATEVLVAGESAGAAAAPLYGGLLADRLSDAQVTVLADGAGAYPNDPAVNELA
                     ISRWGALAAVPDWPGTQGLGAADFGLPTLFVHAGRQHPDLRFGRRDFANDQAQVTYAQ
                     LAGLSDDVAAQIDRNEREIEAQGIDLAGFVSPGEHHTILTTPYLWDHRVGGVMLVDWI
                     QSLIDGTAVDVR"
     gene            complement(769..1851)
                     /locus_tag="PLP95_07690"
     CDS             complement(769..1851)
                     /locus_tag="PLP95_07690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
                     /protein_id="HPB45717.1"
                     /translation="MLVKAAVLREPQAPYTIEDVELDEPGPGQVRVRIVGAGICHTDV
                     VPRGVSLSPLPIVTGHEGSGVVDAVGEGVEGIAAGDHVVLTFDSCGECEQCRENHPAY
                     CETFLMRNLVGRELDGTTCMHDAGGEEVSARWFGQSSFATFAIANARNTVVVDKDLPL
                     DKLGPLGCGILTGAGSIVSAMDVQPGRSLVVTGAGAVGLAAIMAAKVIGANPIIAVDL
                     HESRLELAKELGATHTVNGAGDVTAEVLAITGAGAHYAFDTTGVPAVIQTALASLRMS
                     GVLGLVGVQQGEVVLDGFTQLGKTIMTIFEGDVVPQEMIPKLIGWWRNGEFPFDRLIE
                     TFPFDQINEAEEASLSGRVVKPVLLF"
     gene            complement(2062..3177)
                     /locus_tag="PLP95_07695"
     CDS             complement(2062..3177)
                     /locus_tag="PLP95_07695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB45718.1"
                     /translation="MGKRLRRVGAVAIAAAVTASACVAEAPASSRDEEVAKMVRFVER
                     KRGREFLTPPVVSFVPEQEFVARVHEMVDDTAATLQVDEAAFKALGWMPEADALVDKY
                     RIAFGNGVMGFYDPITEELAVRGDKLTPYRREVVVHELTHALDDQHFGLDEEHGEGII
                     DSRQLAFLVAVEGDAAAAQRSWSQYRGPIEASASLLEQLSMPVSAELLGVPIALLSLA
                     QAPYLQGPRFIRALDGEIDAMFSAFPVSEEQAWFPDRYLLGDQPQDVPDPIAGGVEIR
                     RGEFGVFLLTLLIQRGISLDAAVNSATSGWSGGEYVTWDGGGSRRCLTATVRMDTEEQ
                     AAGLRAALSGWAASAPSATLSALGADTTIESCSSAGG"
     gene            complement(3202..>3439)
                     /locus_tag="PLP95_07700"
     CDS             complement(3202..>3439)
                     /locus_tag="PLP95_07700"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020376860.1"
                     /note="catalyzes the phosphorylation of
                     N-acetyl-L-glutamate to form N-acetyl-L-glutamate
                     5-phosphate; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HPB45719.1"
                     /translation="LLGDIDDPSSLITEVSTSQARQLIDDGTIGGGMIPKIEGCIEAI
                     HRGVREVHLIDGRVPHVLLLELFTDAGVGTMVIA"
BASE COUNT          584 a         1242 c         1066 g          547 t
ORIGIN      
        1 cgactggtcg atggtgttcg tgccctactg caccggcgat ctgcacctcg gagacgccgt
       61 gcgcgaggac tcccccgaac aggtcgtcca ccactccggc ctggcgaacg gcaccgccgt
      121 gctcgatcag gtcgaagaac agttccagac cgccaccgag gtgttggtcg ccggcgagag
      181 cgccggggcc gcggccgccc cgctgtacgg cggcttgctg gccgaccggc tttcggacgc
      241 ccaggtgacg gtgctcgccg acggcgccgg cgcgtacccg aacgatccgg cggtcaacga
      301 actcgcgatc agccggtggg gcgcgctcgc ggcggtcccc gactggcctg gaacccaagg
      361 gctcggcgcc gccgacttcg gactaccgac cctgttcgtg cacgctgggc gccaacaccc
      421 cgatctccgc ttcggacgac gcgatttcgc gaacgaccag gcccaggtga cctacgcaca
      481 actcgccggc ctgagcgacg atgtcgccgc acagatcgac cgcaatgaac gcgagatcga
      541 ggcccagggc atcgacctcg cgggctttgt ttcaccgggc gagcatcaca cgatcctcac
      601 cacgccgtac ttgtgggacc accgggtcgg cggcgtcatg ttggtcgact ggatccagtc
      661 gctgatcgac ggaaccgcgg tcgacgtgcg ctgaccgggt gctgaccgga caccgctgac
      721 cgggcgccgc cgaccgggcg ccgccgaccc ggcttcagaa gaggagcttc agaagaggag
      781 tacgggcttc acgacccgcc ccgaaagcga cgcctcctcg gcctcgttga tctgatcgaa
      841 cgggaaggtc tcgatcagcc ggtcgaaggg gaactcgccg ttacgccacc acccgatcag
      901 tttcgggatc atctcctgag gcaccacgtc gccctcgaag atcgtcatga tcgtcttgcc
      961 gagttgtgtg aagccatcca gcaccacctc gccctgctgc acgccgacca gacccaacac
     1021 cccggacatt cgcagcgagg ccaacgccgt ctggatcacc gccggcaccc ccgtggtgtc
     1081 gaaggcgtag tgggctcctg caccggtgat ggcgagcacc tcggcggtca cgtcgccggc
     1141 gccgttcacc gtgtgggtgg cgccgagttc cttcgcgagt tcgaggcgcg actcgtgcag
     1201 gtcgaccgcg atgatcgggt tcgcgccgat gaccttggcc gccatgatcg ccgccaaccc
     1261 gaccgctccg gcaccggtca cgaccaggct gcggcccggt tggacatcca tcgccgaaac
     1321 gatggatccg gcaccggtca aaatgccaca gccgagcggg ccgagcttgt cgagcggcag
     1381 gtccttgtcg acgacgacgg tgttgcgagc attcgcgatg gcaaaggtcg cgaacgacga
     1441 ctgaccgaac caacgcgccg agacctcctc gcccccggcg tcgtgcatgc acgtcgtccc
     1501 gtcgagttca cggcccacga ggttacgcat gaggaacgtt tcgcaatacg ccggatggtt
     1561 ctccctgcac tgctcgcatt caccgcagga atcgaaggtc aacacgacgt gatctccggc
     1621 cgcgatcccc tcgacccctt cgcccaccgc atcgacgacg cccgaaccct catggccggt
     1681 gacgatcggc agcggcgaca acgacacccc ccggggaacg acgtcggtgt gacagattcc
     1741 ggcaccgacg atgcgtaccc gcacctgacc cgggccgggt tcgtcgagct cgacgtcctc
     1801 gatcgtgtac ggagcctgcg gctcgcgaag aaccgctgct ttcaccaaca tgtcaggtac
     1861 cccctcattg cagtcgtgtc attgcagtcg tgtcgctgca gtcgtgtcat tgcagtcgtg
     1921 tcgctgcagt cgtgtcgctg cattcgtgtc gctgttgtgt ggcactgtaa cggccagcgc
     1981 tgccctaccc gaacggccaa cggcgccctg cccgaacggc caacggcgcc ccgcctgaac
     2041 ggccagcgtg agcgtcccgg cctatccgcc ggcggagctg cacgattcga tcgtggtgtc
     2101 ggccccgagt gcactcaatg tcgccgacgg tgccgacgcg gcccatccac tcaacgcggc
     2161 gcgcaggccc gcggcctgct cctcggtatc catgcgcacg gtcgcggtca ggcaccggcg
     2221 tgaccctccg ccgtcccagg tcacgtactc gccaccgctc cacccgctcg tcgcggaatt
     2281 gaccgcagcg tcgagactga tgccccgttg gatgagcagc gtgagcagga acacgccgaa
     2341 ctcgccgcga cggatctcga cgccaccggc gatcggatcg ggcacgtcct ggggctgatc
     2401 gccgagaagg taacgatccg ggaaccacgc ctgttcctcg ctcaccggaa acgccgagaa
     2461 catggcatcg atctcgccat cgagtgcccg gatgaaccgt ggaccctgca ggtagggggc
     2521 ctgggccaag ctcagcaagg cgatcggaac gccgagcaac tcggcgctga ccggcatcga
     2581 cagctgttcg agcaagcttg cggaggcctc gatcgggcca cggtactgcg accaggaccg
     2641 ttgagccgcg gccgcgtcgc cctcgaccgc gacgagaaac gccaactgcc gcgaatcgat
     2701 gatgccctca ccgtgttcct cgtcgaggcc gaagtgctga tcgtcgagcg catgggtcag
     2761 ttcgtgcacc acgacctcgc gccgatacgg ggtcagcttg tccccgcgca ccgccaactc
     2821 ctcggtgatc gggtcgtaga accccatgac gccgttgccg aacgcgatcc ggtacttgtc
     2881 gaccagggca tcggcctcgg gcatccaccc gagcgccttg aacgccgcct cgtccacctg
     2941 cagcgtggcg gctgtgtcgt cgaccatctc gtgcacgcgg gcgacgaact cctgctccgg
     3001 cacgaagctc accaccgggg gcgtcaggaa ttcgcggcca cgcttccgct cgacgaaccg
     3061 aaccatcttc gcgacttcct catcgcggct cgacgccggc gcctcggcca cacatgcgct
     3121 cgcggtgacc gctgcggcga tcgccaccgc gccgacgcgc ctgagacgct tccccatgtt
     3181 cttccccctt cgaatgccga ttcaggcgat gaccattgtg ccaactccgg cgtcggtgaa
     3241 cagttccaac aacaacacgt ggggcacccg accgtcgatc aggtggacct cgcgcacccc
     3301 gcggtggatg gcctcgatgc acccctcgat cttggggatc atcccgccgc cgatcgtgcc
     3361 gtcgtcgatc aactgccgcg cctgcgaggt cgacacctcg gtgatcaggc tcgacgggtc
     3421 gtcgatgtcg cccaacaat
//
LOCUS       DAOOMA010000256         3421 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_33482_length_3421_cov_3.185383, whole genome shotgun sequence.
ACCESSION   DAOOMA010000256 DAOOMA010000000
VERSION     DAOOMA010000256.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 3421)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3421)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3421
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33482_length_3421_cov_3.185383"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..565
                     /locus_tag="PLP95_07705"
     CDS             <1..565
                     /locus_tag="PLP95_07705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020379173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HPB45720.1"
                     /translation="TEYFSMVRALQSVDEADAALLVIDATEGITHQDQRLAERIDAAG
                     SPIVVLLNKWETLNEDQRNEVVYQLTQKLHFLGDSAVLRISALTGRGVHRLIPALESA
                     IDAYHRRVPTRKVNDVIRRAQQAQPGPHGVRVLYATQGAIDPPTFTLFANKTIPPHYV
                     RYLERMLREEFDLGQTPIKMRIRKRTD"
     gene            602..1678
                     /locus_tag="PLP95_07710"
     CDS             602..1678
                     /locus_tag="PLP95_07710"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family protein-serine/threonine
                     phosphatase"
                     /protein_id="HPB45721.1"
                     /translation="MLAHWFLFLIAVLAVVGATAWFRSAFLRYRVVRNMTTNDPGVRT
                     LSQTTVLHQVHTGVVYVVIAGAALASLFTDDVRAWYLIGAISVPALFSLWAAQFSKRD
                     ARLSEERVRVEQRALEMLAQNDSAPQRWAERLAPAELIDTPGYDIAVVHEAVAGVMSG
                     DLVDVFRLPSGRVGCVVGDVSGHDVEASITALQTKFLLRSYLRRYRDPGQALEELNRQ
                     VSDYERPEEFVSLFVMIFDEEAEVMRYASAGHPAAWMLHDRELSALPATGPILMMNPE
                     ATFQSREIPIVKGDLVVCTSDGIAEARDGSNFFGEDRVAGMIRKMESATAEQASRSLV
                     NAAQEFVAGPLQDDVTVLAVRRAD"
     gene            1756..3276
                     /locus_tag="PLP95_07715"
     CDS             1756..3276
                     /locus_tag="PLP95_07715"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020379174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA carboxylase subunit beta"
                     /protein_id="HPB45722.1"
                     /translation="MTNPLSIARERASKGNLHSQADKPAGQNKMFVRDRIDLLCDPGT
                     FVEDALLANAMAQDLPADGVVTGVGEVDGRPVIVVANDPTVKAGSWGARTVEKIVRAT
                     DLALRDNLPIFWLIDSAGARITDQVELFPGRRGAGRIFYNQVRLSGKVPQICCLFGPS
                     AAGGAYIPAFCDIVIMVDGNASMYLGSPRMAEMVIGETASLEETGGARMHCSVSGCGD
                     NLAVDDADAIDQAKAYFTYMPSYWRDEPPEYEPAAPVRELDSTVVPAEETMGYDIHTV
                     IDALIDAESFFEVKALFAPELVVGFGLMEGRPVGFVANNPMIKGGVLFTDTADKAARF
                     IWCCDAFNIPLVFLADVPGFMIGTEVERQGIIRHGAKMITAVSEATVPKISVVLRKAY
                     GAGLYAMSGPAFEPEATIALPTAKIAVMGPEAAVNAVFANKIAAIEDEVERDAFVAEQ
                     RRIYEQDVDLLRLASELVIDAVVDWGDLRLEIIRRLDRAKGKTREFSDRRHGVPPV"
BASE COUNT          598 a         1117 c         1113 g          593 t
ORIGIN      
        1 caccgaatac ttctcgatgg tgcgcgccct gcaatcggtt gacgaggccg acgccgcgct
       61 gttggtcatc gacgccaccg agggaatcac ccatcaggat cagcggctgg cagaacgcat
      121 cgacgccgcc ggcagcccga tcgtcgtgct gctcaacaaa tgggagaccc tcaacgagga
      181 ccagcgaaac gaagtcgtct accagctgac ccagaagctg cactttctcg gcgactcggc
      241 ggtgctgcgg atctcggcgc tcaccggccg cggagtccac cgattgatcc cggcgctcga
      301 aagtgcgatc gacgcctacc accgccgggt gcccacccga aaggtcaacg acgtcatccg
      361 gcgcgctcaa caggcgcaac ccgggcccca cggagttcgg gtgctctatg cgacccaggg
      421 cgcgatcgat cctcccacct tcacgctgtt cgcgaacaag acgattcctc cgcattacgt
      481 tcgctacctt gaacgcatgc tccgagagga gttcgacctc ggacagaccc cgatcaagat
      541 gaggattcgc aagcgcaccg attgatcggc tgcccgacga acgtatcgaa gcggtattcc
      601 catgctcgcc cactggtttc tcttcctgat cgccgtgctc gccgtcgtcg gcgccaccgc
      661 gtggtttcgc tcggcgttcc tgcgctatcg agtggtccgc aacatgacca ccaacgaccc
      721 cggggtacgt accctcagcc aaaccaccgt gttgcaccag gtccataccg gggtcgtgta
      781 cgtggtgatc gccggggccg ccctcgcctc gctgttcacc gatgacgttc gggcctggta
      841 cctcatcgga gcgatttcgg ttccggcact gttcagcctc tgggcggcgc agttctccaa
      901 acgcgacgct cgtctgagcg aagagcgggt gcgggtcgaa cagcgggccc tcgaaatgct
      961 ggcccagaac gactcggcgc cgcaacggtg ggccgagcgc ctcgccccgg ccgaactcat
     1021 cgacaccccg ggctacgaca tcgcggtcgt tcacgaggcg gtggccggcg tgatgtcggg
     1081 cgaccttgtc gacgtgtttc ggctgccctc tggacgcgtc gggtgtgtgg tcggcgacgt
     1141 gagtggccac gacgtcgagg cgtcgatcac ggcactgcag acgaaattcc tgttgcgctc
     1201 ctacctgcgc cgctaccggg atccggggca ggcgctcgaa gagctcaacc ggcaggtttc
     1261 cgactacgaa cggcccgagg agttcgtgtc gctgttcgtc atgattttcg acgaggaagc
     1321 cgaggtgatg cgctacgcgt cggccgggca ccccgcagca tggatgctgc acgaccgcga
     1381 actgagcgcg ttgccagcca ccggtccgat cctgatgatg aaccccgagg cgaccttcca
     1441 atcccgcgag attccgatcg tcaagggcga cctcgtggtg tgcacctccg atggcatcgc
     1501 cgaggctcgc gacggttcga acttcttcgg agaggaccgg gtggccggca tgatccgaaa
     1561 gatggagtcc gccaccgccg aacaggcgag ccgcagtttg gtgaatgccg cacaggaatt
     1621 cgtcgccggg ccccttcagg acgacgtcac ggtgttggcg gtccgccgag cggactgagc
     1681 tcggccggcc cagcggctcg acctgcgacg gagcggactg ggcgccgggc cgaggagttc
     1741 tgcgaaactc aacggatgac gaacccgttg tcgatcgccc gcgaacgagc ctcgaagggc
     1801 aacctgcatt cccaggccga caagcccgca gggcagaaca agatgttcgt gcgcgaccgc
     1861 atcgacctgc tgtgtgaccc cggcaccttc gtcgaggatg ccctgcttgc caacgccatg
     1921 gcccaggacc tgccggccga cggcgtggtc accggggtgg gagaggtcga cggacggccg
     1981 gtgatcgtcg tggccaacga cccgacggtc aaagccgggt cctggggtgc tcgcaccgtc
     2041 gaaaagatcg tgcgagcgac ggacctggcg ttgcgcgaca acctgccgat cttctggctc
     2101 atcgattcgg ctggggcgcg gatcaccgac caggtcgagc tgttccctgg gcggcgcggc
     2161 gccggacgca tcttctacaa ccaggtgcgc ctgagcggaa aggttcccca gatctgttgc
     2221 ctgttcggcc cctcggccgc cggaggtgcc tacatcccgg cgttctgcga catcgtgatc
     2281 atggtcgatg gcaatgcctc gatgtacctg ggttcgcctc gcatggccga gatggtgatc
     2341 ggcgagaccg cgagcctcga ggagaccggt ggggcccgga tgcactgttc ggtgtcgggc
     2401 tgcggtgaca accttgccgt cgacgacgcc gatgccatcg accaggcgaa ggcgtatttc
     2461 acctacatgc cctcgtattg gcgcgacgag ccgcccgagt acgaacccgc tgcgccggtt
     2521 cgtgaactcg attcgaccgt cgtgccggcc gaggagacga tgggttatga catccacacg
     2581 gtcatcgacg cactgatcga cgccgagagc ttcttcgagg tcaaggcgct gttcgctccc
     2641 gagttggtgg tcgggttcgg tttgatggag gggcgtcccg tcggctttgt cgccaacaat
     2701 ccgatgatca agggtggggt gctgttcacc gacaccgccg acaaagctgc ccggttcatc
     2761 tggtgctgcg acgccttcaa catcccgttg gtcttccttg cggacgttcc gggtttcatg
     2821 atcggcaccg aggtcgagcg tcagggcatc atccgccacg gcgccaagat gatcaccgcc
     2881 gtgagcgagg cgacggttcc caagatctcg gtggtgctgc gcaaggcgta cggcgccggc
     2941 ctctacgcca tgtccgggcc ggcgttcgag cccgaagcga ccatcgcctt gcccaccgcg
     3001 aagatcgcgg tgatggggcc cgaggcggcg gtcaacgccg tgttcgccaa caagatcgcg
     3061 gcgatcgaag acgaggtcga gcgcgacgcg ttcgtcgccg aacagcgacg catctacgaa
     3121 caagacgtcg acctgctgcg cctcgcctcg gagttggtga tcgacgccgt ggtcgactgg
     3181 ggcgacctgc ggttggagat catccgtcgg cttgaccggg caaagggcaa gacccgcgaa
     3241 ttcagcgacc gacgtcacgg cgtgccgccg gtgtgactgg atggcgtgcc gccggtgtga
     3301 ccgcgatacg gcggcagtag taaacatggc caacgatgcc ctggtgcgat gactgcgatc
     3361 acttctacac gccaagcacg ctcaatgcgc aggggcagtg ccccaacggt caccaggtgg
     3421 t
//
LOCUS       DAOOMA010000257         3411 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_33611_length_3411_cov_3.961263, whole genome shotgun sequence.
ACCESSION   DAOOMA010000257 DAOOMA010000000
VERSION     DAOOMA010000257.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 3411)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3411)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3411
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33611_length_3411_cov_3.961263"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..584)
                     /locus_tag="PLP95_07720"
     CDS             complement(<1..584)
                     /locus_tag="PLP95_07720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012227972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HPB45723.1"
                     /translation="MDLTWTSDEQAFRDEARAWLEANVPNPPLPSGDTAEGFALHLEW
                     ETKLFESRWAAVSWPTEYGGRAASLWEWLIFEEEYYRVGAPQRVTQNGIFLLAPTIFE
                     FGTQAQQDYYLPRMAAGIDLWAQGWSEPNAGSDLAGLTSKAVRDEENGGWRLYGQKTW
                     TTRGAFCNYLFGLFRTDPDSERHKGLTYLLVPLD"
     gene            complement(628..1716)
                     /locus_tag="PLP95_07725"
     CDS             complement(628..1716)
                     /locus_tag="PLP95_07725"
                     /EC_number="1.13.12.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012227967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase family protein"
                     /protein_id="HPB45724.1"
                     /translation="MSEPQGSARPAALSTAVCELFGTRYPIVQTGMGWVSGARLTAAT
                     CAAGGLGILASATMTLPELRTAIAKVKERTLNPFGVNMLPNQSDLSERIDLLVAEGAR
                     VASFAGAPSADVVARLNDAGIVSIVTVGAKRHAEKMVKIGVSVLIAQGGEGGGHTGST
                     PTSLLLPAVVDATEGSGVPVLGAGGYYDGRGLVAALAYGAQGIAMGTRFLLTRESHVP
                     DHVKSIYVDTPVTGTVVTEAIDGAPQRVIRTEMIDRIERKPGFAQFPVAAKNALAFAK
                     LSGTPLKDLLKSGLEMRKTQDLTWAQMALAANAPMMTRAGVVDGKVEAGILPTGQVVG
                     AIDELPSVEELLERIMDEAAATLRRLGA"
     gene            complement(1713..2516)
                     /locus_tag="PLP95_07730"
     CDS             complement(1713..2516)
                     /locus_tag="PLP95_07730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020379401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-transferase"
                     /protein_id="HPB45725.1"
                     /translation="MSTTSTTTSSEILDGPPTRAEVCVVAVAEAFRGDGERLCNPIGT
                     IPMVGGRLARETFEPDLVMTDGFGAFAANPLPPGADPADAVVEAYNPYANMFTVLWNG
                     KRHVMMGATQVDIWGNQNIACIGDWHKPRNQLLGFRGAPGNTIHHTTSYWVPNHNPKV
                     FVPAVDVVCGIGYDRARELGPVASRFHEIRRVVSNLGVFDFETPDNRMRLRSLHPGVT
                     VDEVVAATGFELVIPDDVAESRMPTDEEIRLIREVIDPRGLREKEVPNP"
     gene            complement(2513..3391)
                     /locus_tag="PLP95_07735"
     CDS             complement(2513..3391)
                     /locus_tag="PLP95_07735"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020379402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-transferase"
                     /protein_id="HPB45726.1"
                     /translation="MPMAIPDKRLTEADVVSELRDGMTIGIGGWGSRRKPMSLVREIL
                     RSDLKDLTVVSYGGPDLGMLCREGKVAKAIYGFVSLDSIPLEPHFRNARQNGTVEVEE
                     LDEGMLLLGLQAAAWRVPFLPTRAGMGSDLPRVMPRFKTVRSPYPQADGSEGEELLAM
                     PALELDVALIHMNRGDQGGNGQFLNQDLFMDDWMALAADKTFMSVEQIVPTEDFPKYG
                     SIHTQRINRLMTTGVVERAGAAHFTECPPDYARDEAFQKEYAASAKSDEAWADFKTKY
                     LDVSEAEYQQLVGVAR"
BASE COUNT          561 a         1171 c         1095 g          584 t
ORIGIN      
        1 tcgtcgagcg gcaccagcag gtaggtgagg cccttgtgac gctcggagtc gggatcggtg
       61 cgaaacagcc cgaacaggta gttgcagaat gctccgcggg tggtccaggt cttttggccg
      121 tacagccgcc agccgccgtt ctcttcgtcg cgcaccgcct tggaggtgag acccgcgagg
      181 tcggaacctg cgttgggctc ggaccatccc tgggcccaca ggtcgatccc ggctgccatg
      241 cgcggcaggt aatagtcctg ctgggcctgg gtgccgaact cgaagatggt gggcgccagc
      301 aggaagatgc cgttctgggt gacccgctgg ggagctccga cgcggtagta ctcctcctcg
      361 aagatcagcc actcccacag gctcgccgcg cgcccgccgt attcggtcgg ccacgacacc
      421 gcggcccagc ggctctcgaa gagcttcgtc tcccattcca gatggagagc gaacccctca
      481 gcggtatcgc cgctcggcaa cggcgggttg ggaacgtttg cttcgagcca ggcgcgggct
      541 tcgtcccgga aggcctgctc gtcggaagtc caggtcagat ccacgcccgc catcatgaca
      601 tttctgacgg atcgtcagga aatggagtca ggcccctaga cgacgcagtg tcgccgccgc
      661 ctcgtccatg atgcgctcaa gcaactcctc gacactcggc aactcgtcga tcgctccgac
      721 gacctgaccg gtcggcaaga taccggcctc gaccttgccg tcgaccactc cagcgcgggt
      781 catcatcggc gcattcgcgg cgagcgccat ctgagcccac gtcagatcct gcgtcttgcg
      841 catctcgagg cccgacttga gcaggtcctt cagcggcgtt cccgacagct tggcgaacgc
      901 caacgcgttc ttcgccgcca ccgggaactg agcaaagccc ggcttgcgtt cgatgcggtc
      961 gatcatctcg gtgcgaatga cccgctgcgg ggcaccgtcg atcgcctcgg tcaccacggt
     1021 gccggtcacg ggggtgtcga cgtagatcga cttcacgtgg tcgggcacat gcgattcccg
     1081 ggtcaacagg aaccgggttc ccatggcgat gccctgggcg ccataggcga gcgccgccac
     1141 caggccgcgg ccgtcgtaat aaccgccggc gccgagcacc ggaaccccgg acccctcggt
     1201 cgcgtcgacc acggccggca acaacagcga cgtcggggtc gagccggtgt gtccgccgcc
     1261 ctcgcctccc tgtgcgatca acacgctgac gccgatcttg accatctttt cggcatgacg
     1321 cttcgccccg accgtcacga tcgacacgat gccggcgtca ttgagccgag ccacgacatc
     1381 ggccgagggg gcgccggcga acgacgcgac ccgggcgccc tcggcgacga gcaggtcgat
     1441 gcgctcggac agatcggact ggttcggcaa catgttcacg ccgaacgggt tcaacgtgcg
     1501 ctccttgacc ttcgcgatcg cggtgcgcag ttcgggcaac gtcatcgtgg ccgaggccag
     1561 gatgcccaac ccgcccgctg cgcacgtcgc tgcggtcaac ctggcgcccg acacccagcc
     1621 cataccggtc tgcacgatcg ggtaccgcgt cccgaatagc tcacagaccg cggtcgacag
     1681 cgctgccggc cgagcggaac cctgcggctc gctcacgggt tcggcacttc cttctcacga
     1741 agcccgcgcg ggtcgatgac ctcgcgaatc aggcggatct cctcgtcggt cggcatgcgc
     1801 gactccgcga cgtcgtccgg aatgaccagc tcgaatcccg tggccgccac gacctcgtcg
     1861 acggtgacgc cggggtgcaa ggaacgcagc cgcatgcggt tgtcgggggt ctcgaagtcg
     1921 aacacgccga ggttcgacac gacgcggcgg atctcgtgga aacgggacgc caccggaccg
     1981 agttcgcggg ctcggtcgta gccgatgccg cacacgacgt cgacggcagg cacgaacacc
     2041 ttcggattgt ggttcggcac ccaatagctc gtcgtgtgat gaatcgtgtt gcccggcgcc
     2101 cctcggaacc cgaggagctg attgcggggc ttgtgccaat cgccgatgca ggcgatgttc
     2161 tggttgcccc agatgtcgac ctgcgtcgcg cccatcatca cgtggcgctt gccgttccac
     2221 aacaccgtga acatgttggc gtacgggttg tacgcctcga cgaccgcgtc tgccgggtcg
     2281 gccccgggcg gaagcgggtt cgcggcgaac gcaccgaagc catcggtcat gaccaggtcg
     2341 ggctcgaagg tctcgcgagc gaggcgcccg cccaccatcg gaatggtgcc gatcgggttg
     2401 cacagccgct cgccgtcacc acggaacgcc tcggcgaccg cgacgacgca gacctcggcg
     2461 cgggtgggcg ggccgtcgag aatctcggaa ctcgtcgtcg tgctcgtcgt gctcatcggg
     2521 ccacccccac gagttgctgg tactccgctt cggagacatc cagatacttg gtcttgaagt
     2581 cggcccacgc ctcatcggac ttggcggatg ccgcgtactc cttctggaac gcctcgtcgc
     2641 gcgcgtagtc cggcgggcat tcggtgaagt gcgctgcacc ggcccgctcg acgacaccgg
     2701 tcgtcatgag acggttgatt cgctgggtgt ggatcgagcc gtacttcggg aagtcctcgg
     2761 tcggcacgat ctgttccacc gacatgaagg tcttgtccgc tgccagcgcc atccagtcgt
     2821 ccatgaacag gtcctggttc aggaactggc cgttgccgcc ctgatcgccc cggttcatat
     2881 ggatcagcgc gacgtcgagt tcgagcgccg gcatggccaa cagctcctcg ccttcgctgc
     2941 catcggcctg ggggtacggc gaacgcaccg tcttgaaccg cggcatcacg cggggaaggt
     3001 ccgaccccat gcccgcgcgg gtgggcagga acggtacccg ccacgccgcc gcctgcagac
     3061 ccagcaacag cattccctcg tcgagctctt ccacctcgac cgtgccgttc tggcgggcgt
     3121 tgcggaaatg tggctcgagc ggaatggagt cgagcgagac gaagccgtag atcgccttgg
     3181 cgaccttgcc ctcgcggcac aacatgccga gatcgggtcc gccgtaggag accacggtga
     3241 ggtccttcag gtcgctgcgc agaatctcgc gcaccagcga catcggcttg cggcgcgaac
     3301 cccatcctcc gataccgatg gtcatgccat ctcgaagttc gctcaccacg tccgcctcgg
     3361 tcaggcgctt gtcgggaatg gccattggca tcctcgtgga tcgttggtca t
//
LOCUS       DAOOMA010000258         3410 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_33629_length_3410_cov_3.665276, whole genome shotgun sequence.
ACCESSION   DAOOMA010000258 DAOOMA010000000
VERSION     DAOOMA010000258.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 3410)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3410)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3410
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33629_length_3410_cov_3.665276"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..876)
                     /locus_tag="PLP95_07740"
     CDS             complement(<1..876)
                     /locus_tag="PLP95_07740"
                     /inference="COORDINATES: protein motif:HMM:NF013630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HPB45727.1"
                     /translation="MNATTRSQPYRIASTVISIAALAVLIAGVPYLLWQLGGWPFPTS
                     VPTVDAIRDALSRPIPDTVIVDVLAIAAWVGWLLFCWSLLTEALAQIRGVALRRARLT
                     SPFHGIARHLIGSITVLAAGLPAVSLTATPAMAEPATTIVTESQITSHDHPPLRLVEP
                     ASRPPDTRDPGPGPNAVAGADYPVVTVERHDSYWAIAERTLGDPMRWREIRNLNVGRT
                     LDDGSVITDATLYSGWKLLLPHDATVDVDTAVPRAMPGLSGSAPDAESTVVVERDDNL
                     WTISEARLEDDLGRDA"
     gene            complement(873..1310)
                     /locus_tag="PLP95_07745"
     CDS             complement(873..1310)
                     /locus_tag="PLP95_07745"
                     /inference="COORDINATES: protein motif:HMM:NF024792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein TadG-related protein"
                     /protein_id="HPB45728.1"
                     /translation="MTRRRVRALAIGGERGTVTVFVVTMTTALLLVAGLVFDGGRMIA
                     ARRDADAVAAAAARAGAQGLDEAGLRATDAAPLDPAAAQARAQRYLAATPYTGNVAVV
                     GDTVSVDVARIQDVSILSLAGVGSSTVTGSGAAIAVRAVTEQP"
     gene            complement(1307..1723)
                     /locus_tag="PLP95_07750"
     CDS             complement(1307..1723)
                     /locus_tag="PLP95_07750"
                     /inference="COORDINATES: protein motif:HMM:NF024792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TadE/TadG family type IV pilus assembly protein"
                     /protein_id="HPB45729.1"
                     /translation="MTAHVAERGSVSIELVLLTPVLVAMLLLVVAFGRIQNARADVEA
                     AARAAARAASTQRDATSARAAGERAAFMEFDGGRFHCDTITFDIDTAAFTADSVVTAT
                     VSCGVALGDVTSMGIPGHHVVAATFSEPVDRYRGTR"
     gene            complement(1720..2109)
                     /locus_tag="PLP95_07755"
     CDS             complement(1720..2109)
                     /locus_tag="PLP95_07755"
                     /inference="COORDINATES: protein motif:HMM:NF019428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein"
                     /protein_id="HPB45730.1"
                     /translation="MRTLGSRGERGDAAVEVVLAVPVLLFLIMLVIQMGLCFHGDQLV
                     EAAAQEGVQAGRVPTGSSTSAETRAHDFLDRLSPSVAASAHVWAARTSEVTRVEVTSR
                     VQQVLPGLDLTVSGSAEAPTERFREDR"
     gene            complement(2112..2300)
                     /locus_tag="PLP95_07760"
     CDS             complement(2112..2300)
                     /locus_tag="PLP95_07760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB45731.1"
                     /translation="MSLIPIIWAFAKARIELARHDERGEITEKVIIIAVFAALAIAVG
                     AIVVAKVTSKAESINLDA"
     gene            complement(2324..3205)
                     /locus_tag="PLP95_07765"
     CDS             complement(2324..3205)
                     /locus_tag="PLP95_07765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB45732.1"
                     /translation="MTGALLLGIAAGGGLMLLATGFLPARVPLDRALDRLDTPAVRER
                     TDDRWWVQALGAPLVDTGIGHAATQSVRRDLRIIGRTTAEHLARCVALVGVALLWAPV
                     TFGLMAVGGVPVSWVLPLWVSLVLSAAAAVVPTLAVRAEATERRRSFRHALGCFLDLV
                     AVRLAGGAGVDSALAGSAAAGEGWAFSELRQALSEARLRGGPSWNGLAELGEEIGIGE
                     LQELAASAGLAGDEGARVRVSIAAKARSIRTRGLADAEGAAQAASERMSLPVVLLMTG
                     FIVFLGYPAVAQVLTGL"
     gene            complement(3202..>3410)
                     /locus_tag="PLP95_07770"
     CDS             complement(3202..>3410)
                     /locus_tag="PLP95_07770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB45733.1"
                     /translation="ISTVTVVTALGLIGLNRSYVQVYATPFGQVVLAGIAAAWGAALW
                     WLASMSRFQQPERFLIAAAEGARR"
BASE COUNT          576 a         1098 c         1222 g          514 t
ORIGIN      
        1 ggcgtcgcgc ccaaggtcat cctcgaggcg cgcttcggag atggtccaga ggttgtcgtc
       61 gcgttcaacc acgacagttg actcggcatc aggcgccgaa ccgctcagcc ctggcatcgc
      121 ccttggcacg gctgtgtcga catcgaccgt ggcgtcgtgt gggagcagga gtttccagcc
      181 ggagtagaga gtggcgtcgg tgatgacgga gccgtcgtcg agggtgcggc cgacgttgag
      241 gttgcggatc tcgcgccagc gcatcgggtc cccaagggtc cgttcagcga tcgcccagta
      301 gctgtcgtgg cgttcgacag tgacgacggg ataatccgca ccggccacag cgttcggacc
      361 tggtccggga tcgcgggtgt cgggaggtct gctcgctggc tcgaccaggc ggagcggcgg
      421 gtggtcgtgc gacgtgatct gggactcggt gacgatggtg gtcgctggct cagccatcgc
      481 cggtgtcgct gtgagcgaga cggcggggag accggctgcg agcaccgtaa tggagccgat
      541 gaggtggcgg gcgatgccgt ggaacgggct ggtgagccgc gcccggcgca acgcgacgcc
      601 ccggatttgt gccaacgcct cggtgagcag cgaccaacag aacagcaacc aaccgaccca
      661 tgccgcgatt gccagcacgt cgacgatgac ggtgtcgggg atcggccggc tgagtgcgtc
      721 gcgtattgcg tcgacagtcg ggacgctggt cgggaacggc cacccgccga gttgccacag
      781 cagatacggg acgccggcga tcagcacggc gagcgcagcg atcgagatga cggtcgaggc
      841 gatccggtag ggctgggagc gtgtggttgc gttcatggtt gctccgtgac ggctcggaca
      901 gcgatggcgg cacccgagcc ggtaacggtc gaggatccga ctccagcgag ggacaggatc
      961 gacacgtcct ggatccgggc gacgtccacg gacacggtgt cgccgacgac cgcgacgttg
     1021 cctgtgtagg gagtcgcggc gaggtagcgc tgcgctcgag cctgcgcggc agcggggtcg
     1081 aggggcgcgg cgtcggtggc gcgcaagccg gcttcgtcga ggccttgtgc tccggctcgg
     1141 gcggcggctg cagcgacggc atcggcgtcg cgacgtgcag cgatcattcg acctccgtcg
     1201 aacacgagtc cagcgaccag cagcagcgca gtggtcatgg tgacgacgaa caccgtcacg
     1261 gtgcctcgct ccccaccgat cgcgagcgct cgaacgcggc gccgggtcac cgggtgcccc
     1321 gataccggtc gactggctcg gagaacgtcg cggcgaccac gtggtgacca ggtatgccca
     1381 tgctcgtcac gtcgccaagc gctactccac acgagacggt cgcggtcacc acagagtcag
     1441 cggtgaaggc ggcggtgtcg atgtcgaacg tgatggtgtc gcagtggaac cgtccgccgt
     1501 cgaactccat gaacgctgca cgttcaccgg cggcacgggc cgaggtcgcg tctcgttgtg
     1561 tcgacgcggc gcgtgctgcc gcccgggcgg cagcttcgac atcggctcga gcgttctgga
     1621 tgcgaccgaa cgccacgacc aggagcagca tggcgaccaa cacaggcgtg agcagcacca
     1681 gttcgatgct caccgaacca cgctcggcga cgtgagcggt catcgatcct cccggaaccg
     1741 ttcggtcggc gcttcggccg agcccgacac ggtgaggtcc agtccgggaa ggacctgctg
     1801 aacacggctg gtgacctcga cccttgtcac ctccgaagtg cgcgcagccc acacgtgcgc
     1861 gctcgcagcg accgacggcg acaagcggtc gaggaagtcg tgggcgcgtg tctcggccga
     1921 cgtagagctg ccggtgggta cccgtccggc ctggacgccc tcctgcgcag ctgcttcgac
     1981 gagttggtca ccgtgaaagc agaggcccat ctggatcacg agcatgatga ggaacaacag
     2041 gaccggcacg gccagcacga cctcgactgc ggcgtcgccg cgttcgccac gggatccgag
     2101 ggttcgcatc gtcaggcatc gaggttgatc gactcggcct tcgacgtgac cttggcgacg
     2161 acgatggcac cgacggcgat cgcaagcgca gcgaacacgg cgatgatgat caccttctcg
     2221 gtgatctcac ctcgttcgtc gtgtcgggcg agctcgatgc gtgctttcgc gaacgcccat
     2281 atgatcggga tgagggacat ggtttcctcc agttggagtt ggttcagagg ccggtgagga
     2341 cctgggcgac ggcggggtag ccgaggaaga cgatgaagcc ggtcatcagc agcacgaccg
     2401 gcagtgacat ccgttcgctc gctgcttgag cagcaccttc ggcgtcagcg agcccgcgcg
     2461 tacggattga tctggccttc gcggcgatgg acacccggac ccgggcgcct tcgtcgccgg
     2521 cgaggccggc cgacgccgcc agctcttgaa gttcgccgat gccgatctcc tcgccgagtt
     2581 ccgcgaggcc gttccacgag ggcccgccgc gtagtcgcgc ttcagatagc gcctggcgta
     2641 gctcgctgaa cgcccacccc tcaccggccg cagcgctacc tgcgagcgcc gagtcgaccc
     2701 ctgcgccgcc agcaaggcgg actgctacga ggtcgaggaa acatccgagg gcgtggcgga
     2761 acgacctgcg ccgctcggtt gcctcggcgc gcaccgccag ggtcggcacg actgctgcgg
     2821 cggccgacaa cacgagcgag acccacagcg gtagcaccca ggagacgggg acaccgccga
     2881 ccgccatcaa accgaacgtc actggcgccc acagcagcgc gactccgacc agcgcgacgc
     2941 agcgggccag gtgctcagca gttgtgcggc cgatgatccg caagtcgcgg cgtaccgact
     3001 gcgtcgctgc gtgcccgatg ccggtgtcga cgagcggtgc gcccagggcc tgcacccacc
     3061 acctgtcgtc ggtccgctcg cgtactgcgg gtgtgtcgag gcgatcgagc gcacggtcga
     3121 gcggcactct cgcagggagg aaccctgtgg cgaggagcat cagacctccg ccggcggcga
     3181 tccccaggag aagtgcgccg gtcatcgacg tgctccttcg gctgcggcga tgaggaaccg
     3241 ttcgggttgt tggaagcggc tcatcgaggc gagccaccaa agcgccgcac cccaagctgc
     3301 ggcgatcccg gcgagaacga cttggccgaa cggggtggcg tacacctgca cgtaggagcg
     3361 gttgaggccg atcagtccca gcgcggtgac gacggtgacg gtggagatga
//
LOCUS       DAOOMA010000259         3401 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_33756_length_3401_cov_3.361327, whole genome shotgun sequence.
ACCESSION   DAOOMA010000259 DAOOMA010000000
VERSION     DAOOMA010000259.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 3401)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3401)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3401
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33756_length_3401_cov_3.361327"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..530
                     /locus_tag="PLP95_07775"
     CDS             <1..530
                     /locus_tag="PLP95_07775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="His/Gly/Thr/Pro-type tRNA ligase C-terminal
                     domain-containing protein"
                     /protein_id="HPB45734.1"
                     /translation="PKISVQARDAIGRSWQMSTIQVDLMLPQRFGLEYVGADGNRHRP
                     VMIHRALFGSVERFFAVLLEHYAGAFPTWLAPVQVRILPVADAHTDYAFEVAGLLGES
                     GIRVDVVGADETLGNRIRKAKGEKLPYVLVVGDDDVAARTVGVNARGRDVERDVAVDE
                     FARRVLDEVAERRNA"
     gene            551..1078
                     /locus_tag="PLP95_07780"
     CDS             551..1078
                     /locus_tag="PLP95_07780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT domain-containing protein"
                     /protein_id="HPB45735.1"
                     /translation="MALDRLWATWRSNYVTGIADTRSEDPADGRTLFERILAAPGSDS
                     DKHIVARGEHCFVLLNKFPYTSGHLLVLPNRGEPHLEGLSAREHLELWNLVTASVQAI
                     KAAFRCDGVNVGLNLGAAAGGSQSDHLHVHCVPRWTGDANFMGVAAETRVLPVSLDEA
                     CDRLRAAFVLPKRDA"
     gene            1090..1728
                     /locus_tag="PLP95_07785"
     CDS             1090..1728
                     /locus_tag="PLP95_07785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB45736.1"
                     /translation="MSNETPSKHSTDDDVVAAAAGSLVEPVEVGERVEAAESAEALAP
                     CLDERDIVDELPDDLNAEDFESDYTLPNNNRRRIPAAIYGAMALGCLALWVAFADSSP
                     YVNDGMLWVAVALGVFAIYGFVAGRTLNVDETEALITATRTVGFAVGHASAQMVWRGW
                     LSRPVWRLLVYSSENPPKKRGIVIVDAVDGGVVEWFAEDNPEDWAQPGASGI"
     gene            1833..2549
                     /locus_tag="PLP95_07790"
     CDS             1833..2549
                     /locus_tag="PLP95_07790"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013250.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HPB45737.1"
                     /translation="MLDGRFRGTIETWTRPIGRSVKKTGITADQVTFAGLFMSVAAAV
                     TIGAGHLGWGVVLLALTGIPDTLDGAVAKASGTASQRGAFFDSTCDRVTDALLFGGVA
                     WYLMDNPAYHPRAAMLAFAALGSAMLPSYIRAKADALGLDAKGGLVERAERFILLGVG
                     LVIEPLLVPVLVALTVLNMATALQRFVKVWKVADKPQIQPRATRTRRKARAAAKTPAS
                     VRWEARRAAARSRAEARRGS"
     gene            2625..>3401
                     /locus_tag="PLP95_07795"
     CDS             2625..>3401
                     /locus_tag="PLP95_07795"
                     /inference="COORDINATES: protein motif:HMM:NF005919.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylinositol mannoside acyltransferase"
                     /protein_id="HPB45738.1"
                     /translation="MLSRLKGPAAFRAYQLGEVVSRNAPRALVDPSLQLAARLATVRG
                     GDKRWVIERNLRRIYGRDLTRNELNTRVQATYESYARYYYDSFRLPAMDLGQVAEGFT
                     VEGIEHLEAAMDNDPVGPILALPHLGGWEWAAFWITGIRQWKLAAVAERLEPPELFEW
                     FLKFRQSLGMNIIPLGPDAADEVVGAMLDRQIVCLLSDRDLTGSGPEVDFFGERTSLP
                     AGPAVMHLKGGATVLPVAVYFTEQGVHGVVRPPVTIERDPD"
BASE COUNT          548 a         1113 c         1160 g          580 t
ORIGIN      
        1 ccccgaagat ctcggtgcag gcgcgcgatg cgatcggccg gtcctggcag atgtcgacca
       61 tccaggtcga cttgatgctg ccgcagcgtt tcggcctcga atacgtgggc gccgacggga
      121 accgccaccg tccggtgatg attcaccggg ccctgttcgg ctcggtcgag cggttcttcg
      181 ccgtgttgtt ggaacactac gccggggcgt ttccgacctg gttggccccg gtgcaggtac
      241 gcattctgcc cgtcgcggat gctcacaccg actatgcgtt tgaggtggcg ggcctgctcg
      301 gcgagtccgg gatccgcgtc gacgtcgtcg gtgcggacga gaccctcgga aaccggatcc
      361 gcaaggccaa gggtgaaaag ctcccgtacg tcctcgtcgt cggcgacgac gatgtcgcgg
      421 cccgcacggt cggcgtgaac gctcgtgggc gcgacgtcga acgcgacgtc gcggtcgacg
      481 agttcgcccg acgcgtgctc gacgaggtcg ccgaacggcg caacgcgtag gccgcgcggg
      541 atctcacgac atggcactcg atcgactctg ggcgacgtgg cgttccaact acgtcaccgg
      601 catcgccgac acccgctccg aggaccccgc cgacgggcgc acgctgttcg aacggatcct
      661 cgcagcgccg gggtcggatt ccgacaagca catcgtggcg cgaggcgagc actgcttcgt
      721 gctgctcaac aagttcccct acacctccgg gcatctgctc gttctgccca atcgcggtga
      781 gccacacctc gaggggctga gcgcgaggga gcacctggag ttgtggaatc tggtgaccgc
      841 ctcggtgcag gccatcaagg cagcctttcg ctgcgacggg gtgaacgtcg ggttgaatct
      901 cggcgccgca gccgggggga gccagtccga ccatttgcac gtgcactgcg ttccacgctg
      961 gaccggtgat gccaacttca tgggcgtggc ggcggagacc cgcgtgctac cggtgtcgtt
     1021 ggacgaggcc tgcgaccggt tgcgtgccgc cttcgtgttg ccgaagcggg acgcgtaggg
     1081 tggaccctcg tgtcgaacga aaccccctcg aagcactcga ccgacgatga cgtggtcgcg
     1141 gcagcagccg ggtcgctcgt ggagcctgtg gaggtcggcg aacgggtaga ggcggccgag
     1201 tcggctgagg cgctcgcgcc ctgcctcgac gagcgcgaca tcgtcgacga attgcccgac
     1261 gacctcaacg cggaggactt cgaaagcgac tacacgctgc cgaacaacaa ccggcgtcgg
     1321 atccccgccg cgatctacgg ggcgatggcg ctggggtgcc tcgcgctgtg ggtggcgttc
     1381 gccgattcat cgccctacgt gaacgacggg atgttgtggg tagcggttgc gttgggtgtg
     1441 ttcgcgatct acgggttcgt cgccgggcgc acgttgaacg tcgacgaaac cgaggcgctg
     1501 atcacggcga cgcgaacggt cgggtttgcc gtgggccacg cctcggcgca gatggtgtgg
     1561 cgaggctggc tcagtcggcc ggtgtggcga ctgctcgtgt attcgagcga gaaccccccg
     1621 aagaagcggg gcatcgtgat cgtcgatgct gtggacggcg gggtcgtcga gtggttcgcc
     1681 gaggacaatc cagaagactg ggctcagccc ggagcctcag gtatttgacg atttccaccg
     1741 atgtgagtgt tcacatcgtt acgatttccc acagcggaac gctatgctaa ctatagttag
     1801 cgagacggtg aactgctgtt aaaggagaga gcatgctcga cggacggttc cgaggcacga
     1861 tcgagacctg gacgcgaccc attggacgca gcgtcaagaa gaccgggatc accgcagacc
     1921 aggtcacctt cgccggcctc ttcatgtcgg tggcggcggc ggtgaccatc ggcgccggtc
     1981 acctcggctg gggcgtcgtg ctcctcgcac tcaccggaat cccagacacc ctcgacggcg
     2041 ccgtggcgaa ggcctcgggc acggcgagcc aacgtggggc cttcttcgat tcgacgtgtg
     2101 accgcgtgac cgacgcgctg ctgttcggcg gtgtcgcgtg gtacctcatg gacaaccccg
     2161 cctatcaccc acgggccgcc atgttggcct tcgctgcgct cggaagcgcg atgttgccct
     2221 cgtacatccg cgccaaggcc gacgccctcg gcctcgacgc caagggcgga ctcgtcgaac
     2281 gcgccgaacg tttcatcctg ctcggcgtcg ggctcgtgat cgaaccactg ttggtgcccg
     2341 tactcgttgc gctgaccgtg ttgaacatgg cgaccgcgct gcagcgtttc gtcaaggttt
     2401 ggaaggtcgc cgacaagccg cagatccaac cccgcgccac ccgcacgcgc cgcaaggccc
     2461 gcgccgcagc gaagactccg gcgtcggtgc gctgggaggc acgccgtgcg gccgcccgct
     2521 cccgcgccga ggctcgacgc ggcagctgag ttcgctccgc cggagtcgag gtagtcaaac
     2581 ggaccggcag ggtcgcacct gtcggtagtc gatagcatca cgccatgctt tcccgattga
     2641 agggccccgc tgcgtttcgc gcgtaccaac tcggcgaagt cgtgtcgcgc aacgcgccgc
     2701 gggcgctggt cgacccgtcg cttcagttgg cggcacggct ggcgacggta cgcggtggcg
     2761 acaaacgctg ggtgatcgag cgcaacctgc ggcgcatcta tggccgcgac ctgacccgca
     2821 acgaactcaa cacgcgggtg caggccacct acgagagcta cgcgcggtac tactacgact
     2881 cgttccggct ccccgccatg gacctcggtc aggtcgccga aggtttcacc gtcgagggga
     2941 tcgaacatct cgaggcggcg atggacaacg accccgtcgg cccgatcctc gccctgccac
     3001 acctcggcgg ttgggagtgg gcggcgttct ggatcaccgg aatccgccaa tggaaactcg
     3061 cggcggtggc cgaacgcctc gagccccccg agttgttcga gtggtttctc aaattccgcc
     3121 agtcgctcgg catgaacatc atcccgctcg gccccgacgc cgccgacgag gtggtgggcg
     3181 cgatgttgga ccgtcagatc gtgtgtctgt tgagcgaccg cgacctcacc ggaagcggcc
     3241 ccgaggtcga cttcttcggg gaacggacct cgctgcccgc gggaccggcg gtgatgcacc
     3301 tcaagggtgg ggccacggtg cttccggtcg ccgtctactt caccgaacag ggggttcacg
     3361 gcgtggtccg gcccccggtc acgatcgaac gcgaccccga c
//
LOCUS       DAOOMA010000260         3393 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_33868_length_3393_cov_3.565309, whole genome shotgun sequence.
ACCESSION   DAOOMA010000260 DAOOMA010000000
VERSION     DAOOMA010000260.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 3393)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3393)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3393
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33868_length_3393_cov_3.565309"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..238
                     /locus_tag="PLP95_07800"
     CDS             <1..238
                     /locus_tag="PLP95_07800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB45739.1"
                     /translation="ETVPASAPQEPAPAGTETAAPGSASTLAIANYDALAASQVIPRL
                     DGMSSADLDAVRVYERANRGRRTILSRIAQLQDG"
     gene            239..661
                     /locus_tag="PLP95_07805"
     CDS             239..661
                     /locus_tag="PLP95_07805"
                     /inference="COORDINATES: protein motif:HMM:NF024871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HPB45740.1"
                     /translation="MISARLASSNDDRALLELRALARELVGAQRGGPALLDDLAAEER
                     DAPTTVDFVGELDGVVVGYATTVILDQRTRLRELFVHPEAREVGVGHHLLAAAVEHAR
                     ASGAVRLDASALPGDRSTKNFFESHDMKSRLLVVSKQL"
     gene            658..1074
                     /locus_tag="PLP95_07810"
     CDS             658..1074
                     /locus_tag="PLP95_07810"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HPB45741.1"
                     /translation="MSAESSRPEVCVGAVVVSDDALLMIRRGHGPAAGRWSIPSGRVE
                     HGETLAEATVRELAEETGLIGVCSELVGWAESISDLAHFVVFNFEVILLDDTDPVAGS
                     DAAEVQWVPLHAVDQYRLIPGLAEFLHDHGIIEIIT"
     gene            complement(1222..1995)
                     /locus_tag="PLP95_07815"
     CDS             complement(1222..1995)
                     /locus_tag="PLP95_07815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crotonase/enoyl-CoA hydratase family protein"
                     /protein_id="HPB45742.1"
                     /translation="MIDFEKRGHIGIITINRPDARNAVNSDVAQGIEAAIDQVEADDE
                     IFVAILTGAKTDKGYIFCAGADLKQMGVDPAGMNTAKGGFGGFVQRERTKPVIAAVDG
                     PALAGGTELVLAADLVVASKTAVFGIPEVKRSLVAAAGGLFRLPRKIPRNIAMELALT
                     GRLDFPAERAHHFGLVNHLTEEGEALAGAITLAEQICEAAPLAVRESRSIVIECTDQP
                     DEIGWKRSGDGIMKMFTTEDFTEGITAFAEKRAPQWKGR"
     gene            2106..2660
                     /locus_tag="PLP95_07820"
     CDS             2106..2660
                     /locus_tag="PLP95_07820"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HPB45743.1"
                     /translation="MRQFQIAVFDLDGTLIDSDEALVEPFLHLGLSREEITFGHPIEE
                     FCRDRGIDVMAYVEHYDTERVQPFAGATELVEGLGRWAICSNKFNESGYAELDRLGWN
                     PEVALFSRDFGGAEKRLAPVLERLDVEASQVVYVGDSSTDRAVAEEAGCAFVWAGWNP
                     RTAAANPDGTVLSDPRQVLDLLEH"
     gene            complement(2677..3324)
                     /locus_tag="PLP95_07825"
     CDS             complement(2677..3324)
                     /locus_tag="PLP95_07825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB45744.1"
                     /translation="MREGLTRRVDEEHTTWLAELATTLADGRIVRALRLSSRPPKAGA
                     PIPAELIEQLTTQTTENLTLENSQERWAMVIDALAFSPIRSQITPAEAPAEPTEALKA
                     AVAKVATRVPQIAALLGIEASATKRKRPTKARPPKPAAAKPTAEQAAVDEAPTQEVPE
                     AEAPVEAEAPEVEAEAPEVEAEAPEVEAEAPEVEAPVAEAPEVTSADQDSAEQDS"
BASE COUNT          520 a         1125 c         1155 g          593 t
ORIGIN      
        1 cgaaacggtt cctgcgtctg caccgcagga accggcaccg gccggcaccg aaaccgctgc
       61 accgggatcg gcctcgaccc tcgcaatcgc caactacgac gcccttgccg cctcccaggt
      121 catcccgcgg ctcgacggca tgtcgtcggc cgatctcgac gccgtgcggg tctacgagcg
      181 ggccaaccgt ggtcggcgca cgatcctgag ccggatcgcc cagcttcagg acggctgagt
      241 gatttccgcc cggctcgcat cgtcgaatga cgaccgcgcg ctcctcgagc tccgggcgct
      301 cgcccgagag ctcgtcggcg cccagcgggg cggaccggcg ctcctcgacg acttggccgc
      361 ggaggagcga gatgcaccga ccaccgtcga tttcgtcggc gaactcgacg gtgtcgtcgt
      421 cggatacgcg acgacggtca tcctcgatca gcgcacccgg ctacgcgagc tgttcgtgca
      481 tccggaagcg cgcgaggtcg gcgtcggaca tcacctcctc gcggcagcgg tcgagcatgc
      541 acgggccagc ggcgcagttc ggctcgacgc atccgcattg cccggcgacc gttcgaccaa
      601 gaacttcttc gaaagccacg acatgaagtc ccggctcctc gtcgtgtcca agcagctatg
      661 agcgccgagt cgtcgcgtcc cgaggtctgc gtgggggccg tggtggtcag tgacgacgcc
      721 ttgttgatga ttcggcgggg tcacgggccg gcggctggac ggtggtcgat cccgagcggg
      781 cgtgttgaac acggcgagac cctcgccgag gcgaccgttc gcgaactcgc cgaagaaacg
      841 ggtctcatcg gcgtgtgctc ggagttggtg ggctgggcgg agtcgatcag cgatctggcg
      901 cactttgtcg tgttcaactt cgaggtcatc ctgctcgacg atacggatcc cgtcgccggc
      961 tcagatgcgg ctgaggtgca atgggtgccg ctgcatgcgg tggatcagta ccgactcatt
     1021 ccggggttgg ccgagttcct gcacgatcac gggatcatcg agatcatcac ctgaggggcc
     1081 acgcggcacc ctcgtgtcgg cttcgcgtct gggccgccgg ctcggacgcc gaacgcgaag
     1141 atgcccgccg acacagcggc gggcatcttc atcggcggtt gcatgacgcg tcgccgcgac
     1201 atgtggggct cgctgtgcgg atcagcgacc cttccactgc ggagcacgct tttcggcgaa
     1261 cgccgtgatg ccctcggtga agtcctcggt ggtgaacatc ttcatgatgc cgtcgccgga
     1321 gcgcttccac ccgatctcgt cgggctgatc ggtgcactcg ataacgatgg aacgcgactc
     1381 gcgcaccgcc agcggcgccg cctcacaaat ctgctcggcc agcgtgatcg ctcccgccag
     1441 cgcctcgccc tcctcggtca gatggttgac gagcccgaag tggtgagctc gctcggccgg
     1501 gaaatcgaga cgaccggtga gggccagctc catggcgatg ttgcgcggaa tcttgcgggg
     1561 aagacggaac agaccaccgg cggccgcgac taggctgcgc ttgacctccg ggataccgaa
     1621 caccgcggtc ttcgaggcga ccaccagatc ggctgcgagc accagttcgg tgccaccggc
     1681 gagcgccggg ccgtcgacgg cggcgatgac cggcttggtg cgctcgcgct ggacgaagcc
     1741 gccgaaaccg cccttggcgg tgttcatgcc ggccgggtcg acacccatct gcttcaggtc
     1801 ggcgccggcg cagaagatgt agcccttgtc ggtctttgcg ccggtgagga tggcgacgaa
     1861 gatctcgtcg tcggcctcga cctggtcgat ggcggcttcg atgccctggg caacgtcgga
     1921 gttcaccgcg ttgcgggcgt cgggacgatt gatggtgatg atgccgatgt ggccacgctt
     1981 ctcgaagtcg atcatggcca aacgatactt ccgtcttgac cggtcggtct actcatcgga
     2041 ggcacccggc gatcgccgac cggggcggtg tatcggcggg gcgcacgaaa ctgccacact
     2101 cgtgcatgcg ccaatttcag atcgccgttt tcgacctcga tggaaccttg atcgactccg
     2161 atgaggcgtt ggtcgaaccg tttctccatc tgggcctcag ccgcgaagag atcaccttcg
     2221 gccatccgat cgaggagttc tgccgtgacc gcggcatcga cgtgatggcg tacgtcgagc
     2281 actacgacac cgagcgtgtt cagccgtttg ccggggccac agagctcgtc gaaggcctcg
     2341 gccgttgggc gatctgttcc aacaagttca acgagagcgg gtacgccgaa ctcgaccgac
     2401 tcgggtggaa ccccgaggtg gccctgttca gccgtgattt cggtggagcc gaaaagcggc
     2461 tagcgcccgt gctggagcga ctcgatgtcg aagcgtcaca ggtcgtctac gtcggggaca
     2521 gctccacgga ccgtgcggtc gctgaggagg cggggtgcgc gttcgtctgg gcgggatgga
     2581 acccccgcac cgcagcggcg aaccccgatg ggacggtgct cagcgatccc cgacaggtgc
     2641 tcgacctgct ggaacactga gcaccgtgcg ccgcgttcag gaatcctgtt cggcggaatc
     2701 ctgatcggcg gaagtgacct caggtgcctc agccaccggc gcctcaacct caggagcctc
     2761 ggcctcaacc tcaggagcct cggcctcaac ctcaggagcc tcggcctcaa cctcaggagc
     2821 ctcggcctcc accggagcct ctgcctcggg tacctcctgc gtgggggcct cgtcgaccgc
     2881 ggcctgctca gcagtgggct tcgccgcggc cggcttgggc ggccgagcct tcgtcgggcg
     2941 cttacgcttc gtggcgctgg cctcgatgcc gagcaacgcc gcgatctgcg gcacgcgggt
     3001 tgcgaccttg gccacggcgg ccttcagcgc ctcggtcggc tcggcgggcg cctcggccgg
     3061 ggtgatctgg ctgcggatgg gggagaaggc gagtgcgtcg atcaccatcg cccagcgctc
     3121 ctggctgttt tcgagggtga ggttctcggt ggtctgggtc gtcaactgct cgatgagctc
     3181 cgccgggatc ggcgcgccgg ccttgggagg gcgcgagctc agccgcagtg cacgcacgat
     3241 acgtccgtcg gccagcgtcg tggcgagctc ggccagccag gtcgtgtgtt cctcatcgac
     3301 acgtcgggtc agaccctcgc gcaactgcgt ggcgatctcg cggctttcgt cgtcgcgcgc
     3361 cggctgccgg caactcgcct acgagatcga gcg
//
LOCUS       DAOOMA010000261         3388 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_33923_length_3388_cov_3.797780, whole genome shotgun sequence.
ACCESSION   DAOOMA010000261 DAOOMA010000000
VERSION     DAOOMA010000261.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 3388)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3388)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3388
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33923_length_3388_cov_3.797780"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..276)
                     /locus_tag="PLP95_07830"
     CDS             complement(<1..276)
                     /locus_tag="PLP95_07830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB45745.1"
                     /translation="MSDARTVSSGTVSSGTVAQRGAVLAPRFDWAAEAGLADDEVGVL
                     TRLLRIQMFVSEALDNLVAPHGVSVADYMVLASIRRGCTSPAELCRVL"
     gene            complement(273..1535)
                     /locus_tag="PLP95_07835"
     CDS             complement(273..1535)
                     /locus_tag="PLP95_07835"
                     /inference="COORDINATES: protein motif:HMM:NF016777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HPB45746.1"
                     /translation="MSVQPLTKTGARGSELLTSMEEMPWIISVDDHVMEPRDLWQQEL
                     PPSLRDRGPKVVRENVKLTFKGGHYGFERNAPDGDPCDVWVFDDLVMPTGRLHAPAGM
                     DKSVVVNVPAIYEDFRPGTYDRDERLKDMDINRVEAAVNFPNIFPRFCGQGFAEREDK
                     ELALASLRIYNDWMIDDWCGGAGKGRLIPLTLIPLWDPQLAAEEVRRCAAKGSHAIAF
                     SENPSKLGFGTIHSGEWDPLWQACDETDTTITMHIGSSSSMPTTSPDAPLAVSMSLSA
                     INAQGSLLDWIMSGTLDRFADLKVVYAESQVGWMPYLLERADIVWRDGAGDIKHLPNL
                     PSSYVPNRVFGCIFDDQHGLNSRHAVGMESIVFETDYPHADGTFPHSLDVAYRLCINA
                     GMTHDEVHSFLRGNAIRAFGLERFGITE"
     gene            1598..2794
                     /locus_tag="PLP95_07840"
     CDS             1598..2794
                     /locus_tag="PLP95_07840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HPB45747.1"
                     /translation="MSDHYTIISSDTHAGASHAAYREYLDPEYHEAFDEWRGGYSNPF
                     SDLGDERRYRNWDDEMRNGQQDADGVVGEVIFPNTVPPFFPSFVLFARPPKDDEYELR
                     HAGVRAHNRWLADFCSRFPERRAGIGQIFLNDIDDAIADVRWIKENGLRGGILLPTIP
                     PDCDWVLPLNHPDYDRLWAVCEELEVPVNSHGGSGSPAYARHPSSALLMLAEIPAFSR
                     RPFMFMMLSGVFDRFPKLKFVMTEQGTGWLVPTLAQLDEMLAKVRDRGAVGELRFKPE
                     HVLARSATEYFQQNCWVGASFPRATDVAAFRELGIDKIMWGSDYPHDEGTAPFTREAI
                     RQTMSDWPEADLRRVLGENAAEVYGFDLEALAPAAAQFGPTVEEIARPLSELPAEANE
                     ALLQNA"
     gene            2800..3336
                     /locus_tag="PLP95_07845"
     CDS             2800..3336
                     /locus_tag="PLP95_07845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB45748.1"
                     /translation="MTAETPPNSTDTAAADSDSAAGPAAGPTIVSIDDVAWVEVKAQS
                     HDGRRVSIWEKFLEWTPERMVIYARYDPGVVVEKHGHASDHYVFVLSGEVAIGDRICG
                     PGTSITLPLGAVFGPLTAGPEGATMYEVMCGDPRAIPADRDGFVALCAERGIEPLPNP
                     PVPWPTWLGARTDGNPGA"
BASE COUNT          592 a         1084 c         1139 g          573 t
ORIGIN      
        1 aaggacgcga cacaactcgg cgggggaggt gcatccacgc cggatcgagg cgaggaccat
       61 gtagtcggcg acgctcaccc cgtggggggc gacgaggttg tcgagcgcct cggacacgaa
      121 catctgaatc cgcaacaggc gcgtgagcac gccgacctca tcgtcggcca gtcccgcctc
      181 ggcggcccag tcgaagcggg gagcgagtac cgccccccgc tgtgcgaccg tgccgctcga
      241 gaccgtgccg ctcgagaccg tgcgggcgtc gctcactcgg tgattccgaa gcgttcgagc
      301 ccgaaggccc gaatggcgtt gcctcgaagg aacgagtgca cctcgtcgtg ggtcattccg
      361 gcgttgatgc acaggcgata ggccacgtcg agcgagtgcg ggaaggtgcc gtccgcatgg
      421 ggatagtcgg tctcgaacac gatcgactcc atgccgaccg cgtggcggct gttgaggccg
      481 tgctggtcgt cgaagatgca cccgaacacg cggttgggaa cataggaact cggcaggttc
      541 ggcaggtgct tgatgtcgcc ggcaccgtcg cgccacacga tgtcggcgcg ctcgaggaga
      601 tagggcatcc agccgacctg gctctcggca tacacgacct tgaggtcggc gaaccggtcg
      661 agcgtgccgc tcatgatcca gtcgagcagc gaaccctggg cgttgatcgc gctgagggac
      721 atcgacaccg ccagcggggc gtcgggcgag gtggtcggca tggacgacga ggacccgatg
      781 tgcatcgtga tcgtggtgtc ggtctcgtcg caggcttgcc acagcggatc ccattcgccg
      841 gagtggatgg tgccgaaacc gagcttcgac gggttctcgg agaaagcgat cgcgtgcgag
      901 cccttggcgg cgcaacggcg gacctcttcc gcggcgagtt ggggatccca cagcgggatg
      961 agggtgagcg ggatgaggcg acccttgccg gcaccgccac accagtcgtc gatcatccag
     1021 tcgttgtaga tgcgcaacga cgccagcgcg agttccttgt cctcgcgctc ggcgaatccc
     1081 tgcccacaga aacgggggaa gatgttgggg aagttgaccg cggcctcgac acggttgatg
     1141 tccatgtcct tgaggcgctc gtcgcggtcg taggtgcccg gacggaaatc ctcgtagatc
     1201 gccggcacgt tgacgacgac cgacttgtcc atccccgcgg gggcgtgcag ccggccggtg
     1261 ggcatcacaa gatcgtcgaa cacccagacg tcgcacgggt cgccgtcggg ggcgttgcgt
     1321 tcgaagccgt agtggccgcc cttgaacgtg agcttcacgt tctcgcgtac gaccttgggg
     1381 ccacggtcgc gaagggaggg cgggagctcc tgttgccaga ggtcgcgggg ttccatcacg
     1441 tgatcgtcga ccgagatgat ccacggcatc tcctccatcg aagtcagcaa ctcggaaccg
     1501 cgggcgccgg ttttggtcag tggttgaacg gacatctttc cccctcacac atttgggcta
     1561 ccgctaagtt actatgcgga aagcaaaagg gggaaacatg agtgatcact acaccatcat
     1621 ctcgtccgac acccatgccg gagcgagcca cgccgcctat cgcgaatacc tcgaccctga
     1681 gtaccacgag gccttcgacg agtggcgcgg cggctactcg aacccgttca gcgacctcgg
     1741 cgacgaacgg cgctaccgca actgggacga cgagatgcgc aacggccaac aggacgcgga
     1801 tggcgtcgtc ggcgaggtga tctttccgaa caccgtgccg ccgttcttcc cgagtttcgt
     1861 gttgttcgcc cgcccgccga aggacgacga gtacgagctg cgccacgcgg gggtgcgggc
     1921 ccacaaccga tggctggccg acttctgttc gcggtttccc gaacggcgcg cggggatcgg
     1981 gcagatcttc ctcaacgaca tcgacgatgc gatcgccgat gtgcgctgga tcaaggagaa
     2041 cggattgcgc ggtggcatct tgttgccgac gattccaccc gactgcgact gggtgttgcc
     2101 gctgaatcat cccgactacg accgactctg ggccgtgtgt gaggaacttg aggtgccggt
     2161 caacagccat ggcgggtccg gctctccggc ctacgcccga cacccgtcct cggcgctgtt
     2221 gatgctcgcc gagattccgg cgttctcgcg gcggccgttc atgttcatga tgctcagtgg
     2281 ggtctttgac cgcttcccga aactgaagtt cgtcatgacc gaacagggca cggggtggct
     2341 ggttccgacg ctggcccaac tcgatgagat gctggcgaaa gtgcgtgacc gtggtgcggt
     2401 cggcgagttg cggttcaagc ccgaacacgt gttggcacgc tcggcaaccg agtacttcca
     2461 gcagaactgt tgggttggag caagcttccc gagggcaacc gacgtggcgg catttcgaga
     2521 actcggcatc gacaagatca tgtggggcag cgactacccc cacgacgagg gcaccgctcc
     2581 attcacccgg gaggcgattc gccagacgat gagcgattgg cccgaggccg accttcgacg
     2641 cgtcctcggc gagaacgccg cggaggtcta cgggttcgac ctcgaggccc tcgcccctgc
     2701 cgcggcacag ttcgggccga cggtcgagga gatcgcccgg ccgctgagcg aacttcccgc
     2761 cgaggccaac gaagccctcc tgcagaacgc gtagcgacga tgaccgccga aacgcctccg
     2821 aactcaaccg acaccgctgc tgccgattcc gattctgcgg ccggccccgc ggctggcccc
     2881 accatcgtgt cgatcgacga cgtcgcctgg gttgaggtga aggctcagtc tcacgacggt
     2941 cggcgggtgt cgatctggga gaagttcctg gagtggaccc ccgaacgcat ggtgatctac
     3001 gcgcgctacg accccggcgt ggtcgtcgaa aagcacggtc acgcatccga tcactacgtc
     3061 ttcgtgctct ccggtgaggt cgcgatcggc gatcggatct gtgggcccgg gacctccatc
     3121 accttgccgc tcggcgccgt gttcggaccg ctcaccgcgg ggcccgaggg ggcgaccatg
     3181 tatgaggtca tgtgcggaga cccgcgtgcg attccagcgg accgggacgg cttcgttgcg
     3241 ctgtgtgccg agcgtggcat cgaaccgctg cccaacccgc ccgtgccgtg gccgacatgg
     3301 ctgggggccc gtaccgacgg caaccccgga gcctgacccg cggatcgacg gccggtaccg
     3361 tcacatcccc acgctcgacg ccggcatc
//
LOCUS       DAOOMA010000262         3384 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_33981_length_3384_cov_3.641033, whole genome shotgun sequence.
ACCESSION   DAOOMA010000262 DAOOMA010000000
VERSION     DAOOMA010000262.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 3384)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3384)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3384
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33981_length_3384_cov_3.641033"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            350..1714
                     /locus_tag="PLP95_07850"
     CDS             350..1714
                     /locus_tag="PLP95_07850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012222793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HPB45749.1"
                     /translation="MTLRFVIIGGGPAGVQAATVAARLGAKVTLIERDVIGGAANLWD
                     CVPSKAMIATGGAVGELAKAESMGLQPAQGHVDLAALKTRISTITATLERSGRQLLES
                     QGVRIIMGTGRLVGEHRVEAHTVDGAVEEIEADAILVSTGSRPRIPEFAQPDGDRILT
                     TRDAYPPREIPEHLVVIGSGVTGVEFVHMFLSLGSKVTLVVSRNQVLPQKDPEVAAVL
                     EEDFVSRGVKLLKGARAVGIERNDSGVSVICDDGRIAAGSHALLAIGSIPNSEGLGLA
                     EIGVEMDGGYVITVDHHLRTSLPHIYVAGDLSGKLPLSSVASMQGRKIAEHVMGMHIV
                     SHRHLDYDKAASAIFTDPEIADVGLAEADAFAEGRKVRVTKVPFATTAKALINNDSRG
                     FVKLVSDPATGVVLGGSIVGRHAAELISVIAVAVNAGLRVNDIAESLLVHPALAEALM
                     EAAE"
     gene            1727..2251
                     /locus_tag="PLP95_07855"
     CDS             1727..2251
                     /locus_tag="PLP95_07855"
                     /inference="COORDINATES: protein motif:HMM:NF018028.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HPB45750.1"
                     /translation="MFPPDPPHPERTRPIGSFADPAAGTVPTLAVRIGAKIFDLLIQG
                     IPGAVLSAVFVDWGDGDVVAMMASVPLWVQMVSAFVPVLYDFVSVAATGRTFGKALFG
                     IRVVRMVDGGRVDISRSLMRAIVPALGGILALGLPFGGLWIVWQPVVYARVFYDPQRR
                     GLHDRAAGSIVLYD"
     gene            complement(2268..>3384)
                     /locus_tag="PLP95_07860"
     CDS             complement(2268..>3384)
                     /locus_tag="PLP95_07860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="carbamoyl phosphate synthase"
                     /protein_id="HPB45751.1"
                     /translation="GNCVYLGTRDCSAQRRHQKLIEEAPAANIPAEIITQMGEAAVAV
                     AKGCDYVNAGTVEFLYEDGGFFYLEMNTRLQVEHCVSEEVTSKDLVELQLRVASGEPL
                     PFTQDDIEIHGHAIEVRINAENVAEGKFLPSPGKIDKLRVPSGIGVRWDGGYEEGDEI
                     SQYYDNLAGKLVVWAKDRQTAINRTIRALEEFVIEGVATTIPADLAILKAPEFQAFEH
                     STKWVESGLDLTGVGVDPVAASDEDDEPKVRRTVDVEVNGKRFSVAMYLPEGAGFGGG
                     AGAGGGAKKKKAKRGSGAAAGPAKGSGTLVAPMQGTIVKVVAAVGDTVTEGDTICVLE
                     AMKMENAIVADKDGTIATLSVEAGQAVGSGDVLAVIE"
BASE COUNT          496 a         1093 c         1177 g          618 t
ORIGIN      
        1 ctgctcgggt tcccgacggg tgccgccgta gcgcagcgga acgagcgagc gggcagcgcg
       61 cagcgcggtg agcgcgcgtt gagcgcatcg tcgcgccttg gtcccttcgg ggagccgttc
      121 gttcatcgcc tcccgtatcc ggtcccgacg cgtaggagga ggaaccggaa gtgctcgtcg
      181 ctggtcatcg accatgggag tgtggctccg gcagacgacg agtagacgtt cgggcgggga
      241 gcacactgaa acgttagcag ccgtgggttt tcacgttcgg acgttccggc ggcgctagtg
      301 cgtcgcagcg gtgatctcgc ggtgcggtgc cgccggtagc cttggctcca tgacgctgcg
      361 ctttgtgatc atcggtggtg ggcctgcggg ggtgcaggcg gcgacggtgg cggcccgcct
      421 gggcgccaag gtcacgctga tcgaacgcga tgtgatcggc ggcgccgcga atctgtggga
      481 ctgtgtgccc tcgaaggcga tgatcgccac cggaggtgcg gtcggcgaac tggccaaggc
      541 cgagtcgatg gggctgcagc cggcgcaggg ccacgtcgat ctggccgcgc tcaagacccg
      601 catctccacc atcaccgcca ccctcgaacg ttccggtcgt cagctgcttg aaagccaggg
      661 tgttcgcatc atcatgggca ccggacgact cgtgggcgag caccgggtcg aagcgcacac
      721 cgtcgatggg gcggtggagg agatcgaggc cgacgccatc ttggtgtcca ccggatcgcg
      781 cccacggatc cccgagttcg cccagcccga tggtgatcgg attctcacca cacgagacgc
      841 ctacccgccc cgggagatcc ccgaacatct ggtcgtgatc ggctccgggg tgaccggcgt
      901 ggagttcgtg cacatgttct tgtcgctcgg gtccaaggtc accctggtgg tcagtcgcaa
      961 tcaggtgctg ccgcagaagg atcccgaggt ggcggcggtg ctcgaggagg acttcgtcag
     1021 ccgcggggtc aaactgctca agggcgcccg agcggtcggc atcgaacgca acgactcggg
     1081 ggtcagcgtg atctgcgacg acggtcgcat cgctgccggc agccatgcgt tactggccat
     1141 cgggtcgatt ccgaactctg aaggcctcgg cctcgctgag atcggcgtcg agatggacgg
     1201 cgggtacgtc atcacggtgg atcaccacct gcgcacctcg ttgccgcaca tctacgttgc
     1261 cggcgacctc tccggcaagc tgccgctcag ttcggtggcc tcgatgcagg ggcgaaagat
     1321 cgccgaacac gtcatgggca tgcacatcgt gtcgcatcgc catctcgact acgacaaggc
     1381 ggcctccgcg atcttcaccg acccggagat cgcggacgtc ggcctcgccg aagcggacgc
     1441 gttcgccgag ggacgcaagg tgcgggtcac caaggtgccg ttcgccacca ccgcgaaggc
     1501 cctcatcaac aacgacagcc gcgggttcgt caagctggtc agcgacccgg cgacgggcgt
     1561 ggtgctgggg ggctcgatcg tcgggcgtca cgcggcggag ctcatctcgg tcatcgcggt
     1621 ggcggtcaat gccgggcttc gggtcaatga catcgccgaa agcctgctgg tccatccggc
     1681 cctcgcggaa gccctgatgg aggccgccga gtagcctgcg gtacccatgt tcccgccaga
     1741 tccgccgcat ccggaacgca cgcgcccaat cgggagtttc gccgaccccg cagcgggaac
     1801 ggtgccgacc ttggcggtgc gcatcggagc caagatcttc gacctgttga ttcaggggat
     1861 cccgggggcg gtgctgtcgg cggtgttcgt cgactggggc gacggcgacg tggtcgcgat
     1921 gatggcaagt gtgccgctgt gggtgcagat ggtgagcgcg ttcgtgcccg tcctctacga
     1981 cttcgtgtcg gtggccgcca ctggtcggac gttcggcaag gcgttgttcg gtatccgggt
     2041 cgtgcgcatg gtcgacggcg gacgggtgga tatcagccgc tcgctcatgc gcgcgatcgt
     2101 tccggcactg ggcgggattc tcgccctggg gttgcccttc ggcggtttgt ggatcgtgtg
     2161 gcagcccgtc gtgtacgccc gggtcttcta tgacccgcag cgacgcggcc tgcacgatcg
     2221 cgccgccggg tcgatcgtgc tgtacgactg acccggcggc ggcgtgacta ctcgatgacc
     2281 gccagcacgt cgcccgagcc gaccgcctga ccggcctcga cactgagcgt cgcaatcgtg
     2341 ccgtccttgt cggcgacgat ggcgttttcc atcttcatcg cctcgagcac gcagatcgtg
     2401 tcgccctcgg tcaccgtgtc gcccacggcg gcgacgacct tgacgatcgt gccttgcatc
     2461 ggcgccacga gcgtcccgga ccccttcgcc gggccggccg ctgcgccgga accgcgcttg
     2521 gccttcttct tcttggcgcc accaccggct cctgcgcccc cgccgaagcc ggcgccttcg
     2581 gggaggtaca tggcgacgga gaatcgcttg ccgttcacct cgacgtcgac cgtgcggcga
     2641 actttcggct cgtcgtcctc gtcggaggcc gcgaccggat cgacgccgac gcccgtgagg
     2701 tcgagtccgc tttccaccca cttggtggag tgttcgaatg cctggaattc gggggccttc
     2761 aagatggcga ggtcggcggg aatggtcgtc gccacgccct cgatgacgaa ctcctcgagc
     2821 gcgcggatcg tgcggttgat cgcggtctgg cgatccttgg cccacacgac cagcttgccg
     2881 gcgaggttgt cgtagtactg cgagatctcg tcgccttctt cgtagccgcc gtcccaccgc
     2941 acgccgatgc cggagggaac tcggagcttg tcgatcttgc cgggggaggg caggaacttg
     3001 ccctcggcga cgttctcggc gttgatgcgc acctcgatgg cgtggccgtg gatctcgatg
     3061 tcgtcctggg tgaacggcag tggctcgccc gatgcgacac gcagctgcaa ttccacgagg
     3121 tccttgctgg tgacctcttc ggagacgcag tgttcgacct gcaggcgcgt gttcatctcg
     3181 aggtagaaga agccgccatc ctcgtagagg aactcgacgg tgccggcgtt gacgtagtcg
     3241 cagcccttgg cgacggccac cgccgcctca cccatctggg tgatgatctc ggcggggatg
     3301 tttgcggccg gcgcttcttc gatgagcttc tggtgacgtc gctgggccga acagtcgcgg
     3361 gtgccgagat acacgcagtt gccg
//
LOCUS       DAOOMA010000263         3373 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_34121_length_3373_cov_4.178421, whole genome shotgun sequence.
ACCESSION   DAOOMA010000263 DAOOMA010000000
VERSION     DAOOMA010000263.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 3373)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3373)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3373
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34121_length_3373_cov_4.178421"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..755)
                     /locus_tag="PLP95_07865"
     CDS             complement(<1..755)
                     /locus_tag="PLP95_07865"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020379272.1"
                     /note="Catalyzes the transfer of electrons from NADH to
                     quinone; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit D"
                     /protein_id="HPB45752.1"
                     /translation="MNATTDIERLDAAVDRLSTDTSVLRMSATEAEERSGYQSDSERM
                     IMNMGPQHPSTHGVLRLVLEMEGEQVLRSKPVIGYLHTGMEKTGEELTYAQGSTNVTR
                     MDYASPLFSELAFSLTTEKLLGIEVPERAQWIRMLMSEVNRLSSHMLFLATNGMDIGA
                     VSMMIYGWREREELLRFLETVTGLRMNHNYIRPGGVAADLPDGWREMLVPLLDVLPSR
                     LEEFKILMNGQPIWRERLQGVGVLTSEEALALSV"
     gene            complement(761..1315)
                     /locus_tag="PLP95_07870"
     CDS             complement(761..1315)
                     /locus_tag="PLP95_07870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015439970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HPB45753.1"
                     /translation="MSEVTEVVDDDAGTAEKPEPERLHGALVTHGGDATVLHVTPGEY
                     LAVVKALRADGYYMCIDVCGVDYLTHSGRSALPAEIEAQRFEVVAQFLRHSDASRARV
                     RVQVDADEPTVPTITGLFPGAEGPERETWDMFGIVFYGHPDLTRILMPDEWTGHPLRK
                     DYSVGRIPVQFKAAPIRGDREGVK"
     gene            complement(1308..1931)
                     /locus_tag="PLP95_07875"
     CDS             complement(1308..1931)
                     /locus_tag="PLP95_07875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015439969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B family
                     protein"
                     /protein_id="HPB45754.1"
                     /translation="MGIIEGIKNEGVNGIEYNVVTAKVEDLLQWARSRSIWPATFGLA
                     CCAIEMMGAGGAHYDISRFGMEVFRASPRQADVMIVAGRVSQKMAPILRQIYDQMLEP
                     KYVLSMGVCASTGGMFNNYAIVQGVDQVVPVDVYVPGCPPGPETLIHAITTLQNKISS
                     GEVLARRDENGGGAGIVLEQIDGQSASGLTIGRGRGAVAATVGAFDV"
     gene            complement(1931..2434)
                     /gene="ndhC"
                     /locus_tag="PLP95_07880"
     CDS             complement(1931..2434)
                     /gene="ndhC"
                     /locus_tag="PLP95_07880"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012228950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HPB45755.1"
                     /translation="MGQYLPIVILAALAAGFGLVSLVINQLLAPSRPTPAQTAPYECG
                     IVENVEPPTRFPVRFYLIAMIFIIFDIEIVFLYPFTMVFRDAGAFAFLAVVEFAVVVF
                     AAFLYLISSGALDWGPSKKERRSTVLLNNVRAATGIRQVGFEGREVEGRGVDTAAAAS
                     SGEHEAA"
     gene            complement(2447..>3373)
                     /locus_tag="PLP95_07885"
     CDS             complement(2447..>3373)
                     /locus_tag="PLP95_07885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB45756.1"
                     /translation="YGIGLIGRAPAWMITDVNFDRADPMVAVPQTEQLPAHADLPDAA
                     EIMAQYPLVTALAQGAEGEGWEPATITELKTIVQPWATISTAEVMNLSRDAIEKAPDA
                     VAADSATEALINGGGTALRDAVRADANSVREAVDAPLGDWCLLTESDPRRGEAQASAD
                     AALANADAFPGADGETDTTDYLIKNVFLYGGKEPCEPITESSMVKRTWHRVATVFQVK
                     NPDMYAAATAVRSVQHVVPPGGTPPPAESLDDTSEVTVVMLRNLGNKRMIPFVFAVVT
                     FLGFVIFTTMLHYRDKEAMAVRAAFSGAGAGK"
BASE COUNT          644 a         1156 c         1013 g          560 t
ORIGIN      
        1 accgacagcg ccaacgcctc ctccgaggtc agcacgccca cgccctgaag tcgctcccgc
       61 caaatcggtt ggccgttcat gaggatcttg aattcttcga ggcggctcgg caacacgtcg
      121 agcagcggca ccagcatctc gcgccaacca tcgggcagat ccgccgccac accgccgggg
      181 cggatgtagt tgtggttcat gcgcagacca gtcacggtct caaggaaccg caacagttcc
      241 tcacgctcgc gccagccgta gatcatcatc gacaccgcac cgatgtccat gccgttggtg
      301 gcgaggaaca acatgtggct cgagagtcgg ttgacctccg acatcaacat gcggatccac
      361 tgcgcacgct cgggtacctc gatgccgagc agcttctcgg tggtcaacga aaacgccagc
      421 tcgctgaaca gcggcgaggc gtaatccatg cgggtcacgt tcgtggaacc ctgcgcgtag
      481 gtcagttcct cgccggtctt ttccatgccg gtgtggagat aaccgatcac gggcttcgaa
      541 cgaaggacct gttcgccctc catttccagc accaaacgca acaccccgtg ggtggagggg
      601 tgctggggcc ccatgttcat gatcatgcgc tcggaatcgg actgatagcc cgaacgctcc
      661 tcggcctcgg tggccgacat ccgcagcacc gaagtgtcgg tcgacaaacg gtccaccgca
      721 gcgtcgaggc gttcgatatc ggtcgtagcg ttcacggaac tcacttgacc ccctcgcgat
      781 cgccacggat cggagccgcc ttgaactgca ccgggatacg acccaccgag tagtccttgc
      841 gcaacgggtg accggtccac tcgtcgggca tcaggatgcg ggtgaggtcg gggtgaccgt
      901 agaagacgat gccgaacatg tcccaggtct cacgctcggg gccctcggcg ccgggaaaca
      961 ggccggtgat cgtcggaacc gtcggctcgt cggcgtccac ctgcacccgt acccgcgccc
     1021 tcgacgcatc gctgtggcgg aggaactggg cgaccacctc gaagcgctgg gcttcgatct
     1081 ccgccggcaa cgcggagcga ccggaatggg tcagataatc gacgccacac acgtcgatgc
     1141 acatgtagta gccgtcggca cgaagcgcct tcaccaccgc gagatactcg ccaggtgtga
     1201 catgcaacac ggtcgcgtcg ccaccatgcg tgacgagagc gccatgaagg cgctccggct
     1261 caggcttttc ggcagttccg gcgtcgtcgt cgacgacctc ggtgacttca gacatcaaag
     1321 gcccccacgg tggcggctac ggcgccacga ccacgaccga tggtcaggcc cgaggcgctc
     1381 tggccgtcga tctgttcgag cacgatcccg gcgccgccgc cgttctcgtc acgccgggcg
     1441 agcacctcgc ccgaggaaat cttgttctgc aacgtcgtga tggcgtgaat cagcgtctcc
     1501 ggcccaggcg gacaccccgg cacatacaca tccaccggaa cgacctgatc gacgccctgc
     1561 acgattgcgt agttgttgaa catgccgccg gtcgacgcac acacgcccat cgacaacacg
     1621 tacttcggtt cgagcatctg gtcgtagatc tgccgcagga tcggtgccat cttctggctc
     1681 acccggcccg caacgatcat cacatcggcc tgacgcggcg aggcgcgaaa cacctccatg
     1741 ccgaagcggc tgatgtcgta gtgggcgccg ccggcaccca tcatctcgat ggcgcaacac
     1801 gccagcccga acgtcgccgg ccagatcgaa cgcgaacgcg cccactgcag caggtcttcg
     1861 accttcgcgg tgacgacgtt gtactcgatg ccgttgacgc cctcgttctt gattccctcg
     1921 atgatcccca tcaggcggcc tcgtgttccc cgctcgacgc cgcagcggcc gtgtcgaccc
     1981 cgcgaccctc gacctcgcgg ccctcgaatc cgacctgtcg gatgccggtc gcggcacgga
     2041 cgttgttcag caacacggtg ctgcggcgct ccttcttcga cggcccccaa tcgagggccc
     2101 cactcgagat gagatacaga aacgccgcga acaccacgac cgcgaactcg accaccgcca
     2161 agaacgcaaa cgcccccgcg tcacgaaaca ccatcgtgaa cggatacagg aacacgatct
     2221 cgatatcgaa gatgatgaag atcatcgcga tcagatagaa ccgcaccgga aaccgggtcg
     2281 gaggttcgac gttttcgacg atcccgcact catacggagc ggtctgcgcc ggggtggggc
     2341 gcgacggagc cagcaactgg ttgatcacca ggctgacgag cccgaatccg gccgcgaggg
     2401 cggccagaat cacgatcggc agatactgtc ccatcgagtt gtacccctac ttccccgcgc
     2461 ccgctccgct gaacgcagcg cgcacggcca tggcctcttt atcgcggtag tgcagcatgg
     2521 tcgtgaagat cacgaaaccg aggaacgtca cgacggcgaa gacgaacggg atcatccgct
     2581 tgttgccgag gttacgcaac atgaccacgg tgacctcgga ggtgtcgtcg agcgattcgg
     2641 ccggaggcgg ggtgccgcca ggcggaacca cgtgttgcac cgaacgaacc gcggtcgccg
     2701 ccgcgtacat atcggggttc ttcacctgga acaccgtggc gacgcggtgc cacgtgcgct
     2761 tgaccatcga actctcagtg atcggctcgc acggttcctt gccgccgtac aggaacacgt
     2821 tcttgatcag atagtcagtg gtgtcggtct cgccgtcggc accggggaac gcgtcggcgt
     2881 tcgccaaggc cgcatcggcc gaggcctgcg cttcgccgcg tcgcggatcc gactcggtca
     2941 acagacacca gtcgcccagc ggcgcatcga cagcctcacg aacactgttc gcatcggccc
     3001 gcacggcgtc gcgcaacgcg gtgccgccgc cgttgatcaa cgcttcggtg gcggaatcgg
     3061 cggccacggc atcgggcgcc ttctcgatcg cgtcccgaga gaggttcatc acctccgcgg
     3121 tggaaatagt cgcccacggc tgcacaatcg tcttcagttc ggtgatggtc gcgggctccc
     3181 agccctcgcc ttcagcaccc tgagcaagcg ccgtgaccaa cggatattgc gccatgatct
     3241 ccgccgcgtc gggcagatcg gcatgcgccg gcaactgctc ggtctgcgga accgccacca
     3301 tcggatcggc gcgatcgaag ttgacgtcgg tgatcatcca cgccggagcc cgaccgatca
     3361 gaccgatgcc gta
//
LOCUS       DAOOMA010000264         3357 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_34350_length_3357_cov_3.679285, whole genome shotgun sequence.
ACCESSION   DAOOMA010000264 DAOOMA010000000
VERSION     DAOOMA010000264.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 3357)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3357)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3357
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34350_length_3357_cov_3.679285"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..332
                     /locus_tag="PLP95_07890"
     CDS             <1..332
                     /locus_tag="PLP95_07890"
                     /inference="COORDINATES: protein motif:HMM:NF012339.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster binding
                     domain-containing protein"
                     /protein_id="HPB45757.1"
                     /translation="QGQGEGGAGDAEVTAEVTATVNIILKGKRTEIAYQPGDTILETS
                     RRGGLAAPFSCEAGNCATCMALVTEGSATMRVNNALTPEEVAEGWVLTCQALPQGPVV
                     TVEYENF"
     gene            353..586
                     /locus_tag="PLP95_07895"
     CDS             353..586
                     /locus_tag="PLP95_07895"
                     /inference="COORDINATES: protein motif:HMM:NF014790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HPB45758.1"
                     /translation="MDFEFDEHQNDLHEAVREILTKEVPSGVLRKVIDGDSTDALWQT
                     YVSLDWPGLALDVAHGGSGATAVELAIVIEQLG"
     gene            587..1534
                     /locus_tag="PLP95_07900"
     CDS             587..1534
                     /locus_tag="PLP95_07900"
                     /inference="COORDINATES: protein motif:HMM:NF012656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HPB45759.1"
                     /translation="MADPTGFVATTTQFAPLVAACGNDAQRARLLGAVANGGTATIAL
                     GAPSGRWNAETSPVSTRRTDAGWVLEGTACFVLDGDRADEIAVIARTDGSAGGSASSA
                     DGGDIDVFVVPGTAVTATRIETMDPTMHLADVCFDGVVVDEDRRLNGVDPSQRLAAVG
                     RANDEALTGLAAMMVGACQRALDMVVDYVSQRHQFGVPVGSFQAVKHKAVDMHLAIER
                     ARALAYFEALTIAEDDDRRPLAAAMAKAAAGEAQRLVFQHSIQLFGGIGFTWENDIHL
                     YVRRAKALELIFGGAADHRAVVGGLVMAAAFETSTGAAR"
     gene            1531..2724
                     /locus_tag="PLP95_07905"
     CDS             1531..2724
                     /locus_tag="PLP95_07905"
                     /inference="COORDINATES: protein motif:HMM:NF012656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HPB45760.1"
                     /translation="MKLTFDDAIESVRAEFSAWLDANLPTAQMAAQRPRSTADVPAWS
                     RAWQQTMFDAGWLVPGNPPEFGGRNASLLEQFVVLEEMGRRNIAPSMNPQALGIIAPS
                     ILSFGTPEQRQRWAVPILRAEMTAALGMSEPNAGSDLASLRTTAVRDGDDFVLNGQKV
                     WTSGAHDADVILTFVRTDPDAPKHKGISVLLVPTDTPGVERRPFGSIISPDDLDFNEV
                     FFDDARVPAENLLGPLNGGWGVANGSLGHERAMLWLEYSQRLDDFLVSFGRAAHDADM
                     ADDARLLDWYASVAIDAQAMTLLGYRTLAKTRRGVESTEQSILKMFGSEAIQNASVAA
                     VEQLGPLALDQTRLVGEYNHMQMDAYNAGWFERYLRSFAGTIAGGTSEIQRNIVAERV
                     LGLPR"
     gene            complement(2838..3164)
                     /locus_tag="PLP95_07910"
     CDS             complement(2838..3164)
                     /locus_tag="PLP95_07910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB45761.1"
                     /translation="MSKLPAQFSALEPYADWALPSETDRYAKRLASTMDELQAFYDVA
                     FPALTDSVEYLKSVSLEDISEENQNLLWLFSSLVTVSFPVEVWRQPRVPESGAAEVNI
                     IVEPVP"
     gene            complement(3176..>3357)
                     /locus_tag="PLP95_07915"
     CDS             complement(3176..>3357)
                     /locus_tag="PLP95_07915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB45762.1"
                     /translation="LQDANTLEATQTMIENGVVDTFPLCDQEVMLRHLHTVARKYVDD
                     HLSETGTTVRISAAS"
BASE COUNT          597 a         1137 c         1075 g          548 t
ORIGIN      
        1 gccagggcca gggcgagggc ggcgccggcg acgccgaggt gaccgccgag gtgaccgcca
       61 cggtcaacat catcctcaag ggcaagcgaa ccgaaatcgc ctatcaaccc ggcgacacaa
      121 ttcttgagac ctcacggcgg ggcggactgg cagcgccgtt ctcctgcgag gccggcaact
      181 gcgccacctg catggcgttg gtgaccgagg ggtcggcgac gatgcgcgtc aacaacgccc
      241 tcactcctga ggaggtggcc gaagggtggg tcctcacctg ccaggccttg ccacagggcc
      301 cggtcgtgac cgtcgaatac gagaacttct agatcaatca ggagcaacaa gcgtggactt
      361 tgagttcgac gagcaccaga acgacctcca cgaggccgta cgtgagatcc tcaccaaaga
      421 ggtcccgtcg ggcgtgttga ggaaggtgat cgacggcgac agcaccgatg ccctgtggca
      481 gacgtatgtg tccctcgact ggccgggcct ggcactcgac gtcgcccacg gcggctcggg
      541 agcgaccgcg gtcgagttgg cgatcgtgat cgaacaactc ggctaggtgg ccgaccccac
      601 cggctttgtc gccacgacca cacagttcgc acccctcgtc gccgcctgcg gtaacgatgc
      661 acagcgagcg cgtctgctcg gcgctgtcgc caacggtggg accgccacga tcgccctcgg
      721 tgcgcccagc ggacggtgga acgccgagac ctccccggtg agcacccgcc gcaccgacgc
      781 cggctgggtc ttggagggga ccgcgtgctt cgtcctcgac ggcgaccggg ccgatgagat
      841 cgccgtcatc gcccgcaccg acggcagcgc cggcggcagc gcgagcagcg ctgatggcgg
      901 cgacatcgac gtgttcgtgg ttccgggcac agcggttacc gcaacccgaa tcgagaccat
      961 ggacccgacg atgcacctcg ccgacgtctg cttcgacggg gtcgtggtcg acgaggatcg
     1021 acgcctcaac ggggtggacc catcacagcg actcgccgcg gtgggtcggg ccaacgacga
     1081 ggcgctcacg ggtctcgccg ccatgatggt cggggcgtgt caacgggcgc tcgacatggt
     1141 cgtcgactac gtgtcccagc gtcaccagtt cggggtgccg gtcgggtcgt tccaggcggt
     1201 gaagcacaag gccgtcgaca tgcatctcgc catcgaacgg gcgcgtgcgc tggcctactt
     1261 cgaagcactc acgatcgccg aggatgacga ccgccgacct ctcgccgcgg ccatggccaa
     1321 agcagcggcg ggcgaggccc aacgactcgt gttccaacac agcatccagc tcttcggcgg
     1381 catcggtttc acctgggaaa acgacatcca cctctatgtc cgccgggcga aggccctcga
     1441 attgatcttc ggcggcgcgg cagatcaccg ggccgtggtg ggaggactcg tcatggccgc
     1501 cgccttcgaa accagcacgg gagccgcccg atgaaactca ccttcgacga cgccatcgaa
     1561 tcggtccgcg ccgagttctc agcttggctc gacgccaacc tcccgaccgc tcagatggcg
     1621 gcccagcggc cccgttcgac cgcggatgtg cctgcgtggt cgcgggcgtg gcagcagacc
     1681 atgttcgacg ccgggtggct cgttcccggc aacccgcccg agttcggtgg ccgcaacgcc
     1741 tcgctgctcg aacagttcgt cgtgttggag gagatgggtc ggcgaaacat cgccccgtcg
     1801 atgaatcctc aggcgctcgg catcatcgcc ccgtccatcc tcagcttcgg cacccccgaa
     1861 caaaggcagc gttgggcggt gccgatcctt cgcgccgaga tgaccgcagc gctcggcatg
     1921 agcgaaccaa acgccggctc cgacctcgcc agcttgcgca ccaccgccgt gcgcgacggc
     1981 gacgatttcg tcctgaacgg ccagaaggtg tggacctccg gcgcccacga cgcggacgtc
     2041 atcttgacct tcgtgcgcac cgaccccgac gcacccaagc acaaaggcat cagcgtgttg
     2101 ctggttccga ccgacacccc cggggtcgag cgacgcccgt tcggttcgat catctcgccc
     2161 gacgacctcg acttcaacga ggtgttcttc gacgacgcac gcgtacctgc ggagaatctg
     2221 ctcggccccc tcaacggcgg atggggcgtg gccaacggat ccctcggcca cgagcgcgcc
     2281 atgttgtggc tcgaatactc ccagcgcctc gacgacttcc tcgtctcgtt cggtcgagct
     2341 gcgcacgacg ccgacatggc ggatgatgct cgcctgctcg actggtacgc atcggtggcc
     2401 atcgacgcac aggccatgac gctgctcggc taccgaaccc tcgccaagac ccgccgcggt
     2461 gtggagtcga ccgaacagtc gatcctcaag atgttcggtt ccgaggccat ccagaacgca
     2521 tcggtcgccg ccgtcgaaca actgggtccg ctcgcactcg atcagacccg cctcgtcggc
     2581 gagtacaacc acatgcagat ggacgcctac aacgccggat ggttcgaacg gtacctgcgc
     2641 agcttcgccg gcacgatcgc cggtggcacc tcggagatcc agcgcaacat cgtcgctgaa
     2701 cgggtgctcg gcctgccccg ctgaccctca gacctgcccc caaaccgcca gttctccgat
     2761 aaccagtggt cgtgatcacg accactggtt atcggagaac cggttcggga gggagatgat
     2821 caggtcagat tcggggttta cgggaccggt tcgacgatga tgttgacctc ggccgcgcca
     2881 ctctcgggaa cacgcggctg gcgccatacc tcgaccggga acgacaccgt caccagcgag
     2941 gagaacaacc acagcaggtt ctgattctcc tcggagatgt cctccaggct caccgacttc
     3001 aggtactcga cgctgtcggt gagcgccggg aaggccacgt catagaaggc ttgcaactcg
     3061 tccatggtcg aggcgagtcg tttggcgtag cggtcggttt cgctcggcaa cgcccagtcg
     3121 gcgtacggct cgagggcgga gaattgtgca ggaagcttgc tcatcggata ctccttcagc
     3181 tcgccgccga gatgcgaacg gtcgtgccgg tctcgctcag gtggtcatcg acgtacttgc
     3241 gggcgacggt gtgcaggtga cgcaacatca cctcttggtc gcacaacggg aatgtgtcga
     3301 ccacgccgtt ttcgatcatg gtctgggtgg cctcgagggt gttggcgtcc tggagtg
//
LOCUS       DAOOMA010000265         3356 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_34368_length_3356_cov_3.493487, whole genome shotgun sequence.
ACCESSION   DAOOMA010000265 DAOOMA010000000
VERSION     DAOOMA010000265.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 3356)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3356)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3356
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34368_length_3356_cov_3.493487"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..472
                     /locus_tag="PLP95_07920"
     CDS             <1..472
                     /locus_tag="PLP95_07920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB45763.1"
                     /translation="GPAHAAHSDIVQHWYWFRFGHTLGALGTAAMVLAWVRTPDWWFG
                     RFLANKMFMYFGRLSYTLYVWHGLAYLLVFAVTGGDDVSTAFKFARIPLLVAVALAVS
                     LPVYRYVELPVMGMKLRFASEKETLDLRTGKMVATPGRDDAGTAADSGSGEGRL"
     gene            469..1842
                     /locus_tag="PLP95_07925"
     CDS             469..1842
                     /locus_tag="PLP95_07925"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HPB45764.1"
                     /translation="MKLRSAEASNQFESVAPRLGFVPAFDGVRGYGVLIVLLGHLFPE
                     ETDSFNPIVDVFFVISAFLIVTLLLQERRDYGHIALKRFYSRRAIRLLPNSYACMVAW
                     LAIWALVKAVGVELTGEGAEQVNAIPNHVAAAATYSYNLIYPIGGPIGPLGQFWSLSL
                     EEQFYLFAGFGTAFFIATRRRIWVGATVMVALILWIGWSRFNADLGPWPGSEVTTIWW
                     QRGMQLLWLSRPDALLVGVLLALLNAKLPDPLSPQLKKAIHVVGWIGLVACCVVLVSS
                     LKGLYDRGFPFYLPGIPRDASQFANRDGELWCALEPHGPLRPCTDDLWIFRWVFSVMA
                     IAIAPVALCFARYKDSALSKLFCFGWIRKVGEMSYSLYVWHILAFTLAQLLVQGSGRL
                     QSAVVKLVVAFGISWLAHRYIDRAMLGLKLRFASEKVVLDRRTGQEVAIETVTSDDGS
                     APSDSSD"
     gene            complement(1956..2858)
                     /locus_tag="PLP95_07930"
     CDS             complement(1956..2858)
                     /locus_tag="PLP95_07930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012230669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HPB45765.1"
                     /translation="MAELGYEGKVAIITGAGGGLGRAHALELAKRGARIVVNDLGGSV
                     SGEGDDRSAAQRVVDEITAIGGEAVANADSVSSPEGGEAIVQSALDAFGTVDIVVNNA
                     GILRDKTFHNLTPDMIEAVIDVHLLGAFYVTQPAFKVMRENGYGRIINTSSNSGILGN
                     FGQANYGAAKMGLVGFTRVLAAEGAKYNIKANAIAPVALTRMTEELFGDFGKLMSPED
                     ISPIVAWLAHEDCPVTGEVYSVAGGTVSRFFIGLTEGFFKKGHIVEDIRDNWDQIRSE
                     EGYIVPSGPNDELQKLVRILTSQD"
     gene            complement(2969..>3356)
                     /locus_tag="PLP95_07935"
     CDS             complement(2969..>3356)
                     /locus_tag="PLP95_07935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB45766.1"
                     /translation="RGGGFGRRQVTTTTIETTTTTTPEATTTTAPASTTTTSPKTTTT
                     APSTTTSSPEATTTTVVSTSVAEVTTTMVPTTVAQPPDRPDTPLAFTGSSNTTVALIG
                     GALFLGGLSLMVARAAREQRRATTQR"
BASE COUNT          488 a         1004 c         1174 g          690 t
ORIGIN      
        1 cgggcccgcg cacgcggcgc acagcgacat cgttcagcac tggtactggt tccgattcgg
       61 acacaccctc ggggcgctcg gcaccgccgc gatggtcctc gcctgggtcc gcacccccga
      121 ctggtggttc ggtcggttcc tggcgaacaa gatgttcatg tacttcggtc gactctccta
      181 cacgttgtac gtctggcacg gcctggccta tctcctcgtg ttcgcggtga cgggcggcga
      241 cgacgtttcg acggcgttca agttcgccag aatcccgctg ctggttgcgg tggccttggc
      301 ggtgtcgctt ccggtgtatc gctacgtcga gttgccggtc atggggatga agctgcgttt
      361 cgcctcggaa aaggaaaccc tcgacctgcg cacgggcaag atggtcgcca cccccgggag
      421 agacgacgcg ggaaccgctg ccgattccgg atccggggag ggtcgcttgt gaagcttcgt
      481 tcggcggagg cctccaacca gttcgaatcg gtcgcgccgc gcctcgggtt tgtgccggcc
      541 ttcgacggcg tgcggggtta cggcgtcttg atcgtgctgt tgggtcacct gttcccagag
      601 gagaccgatt cgttcaatcc gatcgtcgac gtgtttttcg tcatcagcgc attcctcatc
      661 gtcacgctgt tgttgcagga gcgtcgcgac tacggccaca tcgccctcaa gcggttctat
      721 tcgcggcggg cgatcaggct gctgccgaac tcctacgcgt gcatggtggc ctggttggcg
      781 atctgggcgt tggtcaaggc ggtcggggtc gaactcaccg gggagggcgc cgaacaggtc
      841 aacgcgattc cgaaccacgt cgccgcggcc gcgacctaca gctacaacct gatctacccg
      901 atcggcggac cgatcggacc gctgggacag ttctggtcgt tgtcgttgga agagcagttc
      961 tatctgttcg ccggcttcgg cacggcgttt ttcatcgcga ccagacgtcg catctgggta
     1021 ggcgcgacgg tgatggtcgc gttgatcctg tggatcggtt ggtcacggtt caacgccgac
     1081 ctcggcccct ggccgggcag cgaggtgacg acgatctggt ggcagcgcgg catgcagttg
     1141 ctgtggctct cgcgccccga tgcgctgttg gtcggggtgt tgttggcgtt gttgaatgcc
     1201 aaactgcccg atccattgag cccccagctc aagaaggcga tccatgtcgt cgggtggatc
     1261 ggcttggtgg cgtgttgtgt cgtgctggtg agttcactga aggggctgta cgaccgcggg
     1321 ttcccgttct accttcccgg gatcccgcgc gacgcatccc agtttgcgaa tcgcgacggg
     1381 gaactgtggt gcgcacttga gccccacggg ccgttgcgtc cgtgcaccga tgacctgtgg
     1441 atcttccgat gggtgttctc ggtgatggcg atcgcgatcg ctccggttgc gctgtgtttc
     1501 gcccgctaca aggactcggc gctgtcgaaa ctgttctgtt tcgggtggat tcgcaaggtg
     1561 ggtgagatgt cgtactcgct gtacgtctgg cacattctgg ccttcaccct cgcgcagctt
     1621 ctcgtgcagg ggtcgggccg cctgcagtcc gcggtggtca agttggtggt ggccttcgga
     1681 atctcgtggc tggcgcaccg ctacatcgat cgcgccatgc tcggcctcaa attgcgcttc
     1741 gccagcgaga aggtggtgct cgatcggcgc accggccagg aggtcgcgat cgagacggtg
     1801 acgagcgacg acggcagcgc gccgagcgac tcatccgatt gaggccaaaa cgaacacatt
     1861 catcccactg cggacgacgg tgaggtcgcc cgcagcaaga tgaatgcgcc ggagatggtc
     1921 tcgcgaggtg gtctcgcgag ctccgtcctg ggaggtcagt cctgggaggt caggattcgg
     1981 acgagctttt gcagttcgtc gttcgggccg gagggcacga tgtagccctc ctcgctgcgg
     2041 atctgatccc agttgtctcg gatgtcctcg acgatatggc ccttcttgaa gaagccctcg
     2101 gtgaggccga tgaagaagcg cgacacggtt ccgccggcga ccgagtagac ctcgccggtg
     2161 accgggcagt cttcgtgggc cagccaggcg acgatcgggg agatgtcctc ggggctcatg
     2221 agcttgccga agtcgccgaa cagttcctcg gtcatgcggg tgagggcgac cggggcgatg
     2281 gcgttggcct tgatgttgta tttcgcacct tcggccgcca gcacgcgggt gaagcccacg
     2341 aggcccatct tggccgctcc gtagttcgcc tgaccgaagt tgccgaggat gcccgagttc
     2401 gacgacgtat tgatgatgcg gccgtaaccg ttttcccgca tgaccttgaa cgccggctgg
     2461 gtgacgtaga acgcgccgag caggtgtacg tcgatgaccg cctcgatcat gtcgggcgtg
     2521 aggttgtgga aggtcttgtc gcggagaatg ccggcgttgt tgacgacgat gtcgaccgtg
     2581 ccgaacgcgt cgagcgcgct ttgaacgatg gcctcgccgc cttccgggct cgagacgctg
     2641 tcggcgttgg cgaccgcttc gccaccgatg gccgtgatct cgtcgacgac gcgttgcgcc
     2701 gcgctgcggt catcgccttc gccggagacc gaaccgccga ggtcgttgac gacgatgcgg
     2761 gcgccgcgct ttgcgagttc gagcgcgtgg gcccgtccga gtccgccgcc agcgccggtg
     2821 atgatggcga ccttgccctc gtagccgagt tcagccatgt gtatgcccct ttgcagatgt
     2881 cctgaggccg aacgcctcga gttgaaagcg ggacaaccct aacgcagcgg ccaccgggat
     2941 gatgaagctg ggcgtgacac cggccgggct atcgctgcgt cgtcgctcga cgttgctccc
     3001 gggcggcacg ggccaccatc aggctgagtc cgccgaggaa cagcgctccg ccgatgaggg
     3061 cgacggtcgt gttcgaggaa ccggtgaatg cgagcggggt gtccggccgg tccggcggct
     3121 gagcgaccgt ggtcggcacc atggtggtgg tcacctcggc caccgaggtc gatacgacgg
     3181 tcgtggtggt cgcctcgggg gacgacgtcg tcgtggacgg tgccgtggtg gttgtcttgg
     3241 gtgaggtggt ggtggtcgag gccggcgccg tggtggtcgt ggcctcgggc gtggtcgtgg
     3301 tcgtggtctc gatcgtggtg gtcgtgacct ggcgccgacc gaaaccgcca ccgcgc
//
LOCUS       DAOOMA010000266         3348 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_34471_length_3348_cov_3.840267, whole genome shotgun sequence.
ACCESSION   DAOOMA010000266 DAOOMA010000000
VERSION     DAOOMA010000266.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 3348)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3348)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3348
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34471_length_3348_cov_3.840267"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            247..636
                     /locus_tag="PLP95_07940"
     CDS             247..636
                     /locus_tag="PLP95_07940"
                     /inference="COORDINATES: protein motif:HMM:NF024788.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SHOCT domain-containing protein"
                     /protein_id="HPB45767.1"
                     /translation="MMLAEIGLGDLLWTTIVVFFMISYFMLLFAVLSDVFRDRELGGV
                     SKAIWIVLLLAFPLVTALVYLISRGDGMARRSAKAATDAESEFAEYVQSVSGRSPAGE
                     IARAKELLDAGVIDQAEFARLKAKALG"
     gene            complement(639..1250)
                     /locus_tag="PLP95_07945"
     CDS             complement(639..1250)
                     /locus_tag="PLP95_07945"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HPB45768.1"
                     /translation="MTVRLSRSTDRNALSYRTIAAELEIDPASLYRYFANKKELINAT
                     VDRLWEGVVAQIDPSLPWRRGLEAGADLVFRMVDAHPAVMVEVAQRSTGGPGEYGLIE
                     LILDAIERSGLTGDAVVRAYAVFSGVALSLAANQAAFRLAGEDPDPEPTKAWVSEHLV
                     PDPTRFPHVLHYAVPLMAVTIREVYDLAIANVLDNIEHLVAQG"
     gene            complement(1326..1568)
                     /locus_tag="PLP95_07950"
     CDS             complement(1326..1568)
                     /locus_tag="PLP95_07950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB45769.1"
                     /translation="MVVVRSESPLAEPSPFDESDDEEHPAPSSDSIAIDATAPVNTER
                     LSLFESPIARANLAGADHAGPETGDTMGRSINTRLR"
     gene            1570..3012
                     /locus_tag="PLP95_07955"
     CDS             1570..3012
                     /locus_tag="PLP95_07955"
                     /inference="COORDINATES: protein motif:HMM:NF040438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase domain-containing protein"
                     /protein_id="HPB45770.1"
                     /translation="MSSAPFNPHSPDEVSLVAGGPADIAVPQPAAALPEGYLMEEYFV
                     GGTATSFSAIDMSEDGRWVAEPDAEAPYRTRVIVRRPLDPAAFSGTVVVEWFNVSAIE
                     AAPDWTYLATEIAREGHAYIGVSAQRQGIEGGETILSVEVDDQQAAAAGVDADPSGIK
                     NTDPERYGTLAHPGDAYSFDMFSQVGRVAQASPQSLLGDLVPTQVIAAGESQSAMFMS
                     TLVNAVHPLSPVFDGFLIHSRASLAAPLDGDYAFVRELREGGQGSQFRTTIRDDLDVP
                     VLLFVTETDLTALRYSEVRQPDSARIRTWEVAGTAHADAHLLRALLGGPRDPGIGAIL
                     GCGPINTGPHMEVLTAGFHHLVAWTAGGEAPPTGALLELEPGDEVALARDPRGNALGG
                     VRNPLIDVPIARIDGDPVATEGGSDELCSLFGSTEPFDQATLLELYGSADEYVRRFRE
                     SAAVAVGSGFLLQSEADALIEEAEANRSLF"
     gene            3130..>3348
                     /locus_tag="PLP95_07960"
     CDS             3130..>3348
                     /locus_tag="PLP95_07960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB45771.1"
                     /translation="MMMSTTTRSNRTARISLVAVVLVVGALMAGCVVTPPANQTSRAC
                     VPSGALLPGQAVIAISSASEGSSPRVLSG"
BASE COUNT          516 a         1058 c         1169 g          605 t
ORIGIN      
        1 ctgatggtgg cgccgtaggt gggcgagcct cggtgtgagt gccgggtcgg tgaggatgcg
       61 gaccccgtcg agttcgatca gcgtcgtcgc atgcccgacc caggtgatct cggggcgcgg
      121 gggcgcgaac ggcgacgaca tgaacccgac gctatcaacg gtgttgactt cacgttgatc
      181 gcggagttga cttggcccgc gttcggggcc gaggcggcac gacgcggtgc cgacaggagg
      241 ccgaaaatga tgctcgctga aattggtctg ggcgatctgt tgtggaccac gatcgtggtg
      301 ttcttcatga tcagctactt catgttgctg ttcgccgtgt tgagcgatgt gtttcgcgac
      361 cgtgaactcg gtggggtgtc gaaggcgatc tggatcgtgc tgttgttggc gtttccgctg
      421 gtgacggcgc tggtgtatct catctcccgt ggcgacggga tggcccgacg tagcgcgaag
      481 gctgccacgg acgcagagtc ggagttcgcc gagtacgttc agtccgtatc gggtaggtct
      541 ccggcgggcg agatcgcacg ggccaaggaa ctcctcgatg ccggcgtcat cgaccaggcg
      601 gagttcgccc gtctcaaggc gaaggcgttg ggctgatctc agccctgagc gacgaggtgc
      661 tcgatgttgt cgagcacatt ggcgatcgcc aggtcgtaca cctcgcgaat cgtcaccgcc
      721 atgagcggca ctgcatagtg caacacgtgc gggaaccgcg tggggtcggg gacgagatgc
      781 tccgacaccc acgccttcgt cggctccgga tccgggtctt cgcccgcgag tcggaaggcc
      841 gcctggttcg cagcgagcga caacgcgaca cctgagaaca cggcgtaggc acggaccacc
      901 gcgtcgccgg tcaaccccga ccgttcgatc gcgtcgagga tgagttcgat cagtccgtac
      961 tcgcccgggc cgccggtgga gcgttgggcg acctcaacca tgaccgccgg gtgagcgtcg
     1021 accatgcgaa acaccagatc tgcgccggcc tcgaggccgc ggcgccatgg caacgacggg
     1081 tcgatctgtg ccacgacgcc ttcccacagg cggtcgaccg tcgcgttgat gagttccttc
     1141 ttattggcga aatagcgata cagcgaggcg gggtcgatct cgagttcggc ggcgatggtc
     1201 cggtagctca gcgcgttgcg gtcggtcgaa cgcgagaggc gaaccgtcac gtcgagaatc
     1261 tgctcgcgat ccagaggttg gattcggccc acggtgtccc ttcccgcacg gtgattcccc
     1321 gcgaatcagc ggagtcgggt gttgatgctg cggcccatcg tatcgccggt ttcgggaccg
     1381 gcgtggtccg cacctgctag gttcgcccgc gctatggggg actcgaaaag actcaaacgc
     1441 tccgtgttca ccggggcggt ggcatcgatc gcgatgctgt cgctgctggg cgcgggatgc
     1501 tcttcgtcgt cggactcgtc gaacggcgaa ggctcggcaa gcggcgattc ggaacggacg
     1561 acgaccacga tgtcaagcgc acccttcaac ccacattcgc ccgacgaggt cagcctggtc
     1621 gccggcggcc ccgccgacat cgcggtgcct caaccggcgg cagcgctgcc cgagggctac
     1681 ctgatggagg agtacttcgt cggcggcacc gcgaccagtt tttcggccat cgacatgtcc
     1741 gaggacggcc ggtgggtcgc cgagcccgac gccgaggccc cctatcggac tcgggtgatc
     1801 gtgcggcgac cgctcgatcc ggcggcgttc agcggcaccg tggtggtcga gtggttcaac
     1861 gtcagcgcca tcgaggctgc acccgactgg acctatcttg cgacggaaat cgcccgggaa
     1921 ggtcacgcat acatcggcgt gagtgcgcag cgtcagggca ttgagggcgg cgagaccatt
     1981 ctgtcggtcg aggtcgacga tcaacaggcg gcagcggcgg gagtcgacgc cgaccccagc
     2041 ggtatcaaga acaccgatcc tgaacgctac ggaacccttg cccacccggg tgacgcctac
     2101 tccttcgaca tgttcagcca ggtcggccgg gtggctcagg cctcgccgca gtcgcttctc
     2161 ggcgacctgg tccccaccca ggtgatcgcc gcgggggagt ctcagtcggc gatgttcatg
     2221 tcgaccctgg tcaacgcagt gcatccgctg agccccgtgt tcgacggctt tctcatccac
     2281 agccgagcct cgttggcggc cccgctcgac ggcgattacg cgttcgtgcg tgagttgcgc
     2341 gaaggcgggc aagggtcgca gttccgcacg accattcgcg atgatcttga cgttccggtg
     2401 ttgctgttcg tgaccgaaac cgacctgacc gcactgcgct attccgaggt tcgccagccc
     2461 gacagcgccc ggatccggac ctgggaggtc gccggcacgg cacatgccga tgctcacctg
     2521 ttgcgcgcgt tgctcggagg ccctcgcgac ccgggcatcg gtgcgatcct cgggtgtggg
     2581 ccgatcaaca cgggtcccca catggaagtg ctgacggccg ggttccacca cctcgtggcg
     2641 tggaccgccg gcggcgaggc tcccccgacc ggtgccttgc tcgaactgga gccgggcgat
     2701 gaggtggcgt tggctcgcga cccccggggt aacgcactcg gcggcgtgcg caatccgctg
     2761 atcgacgtgc cgatcgcccg gatcgacggc gaccccgtgg ccaccgaggg cgggtccgat
     2821 gagctgtgct cgttgttcgg aagcaccgaa ccgtttgatc aggcgacgct gctcgagctg
     2881 tacggatcgg cggacgagta cgtgcggcgg ttccgggaat cggccgccgt cgcggtgggg
     2941 tccggcttct tgttgcagtc cgaggccgac gcgctcatcg aggaggccga ggccaaccgc
     3001 tcgctgttct gaccgcggtg accggccgcg gcgacccctg cgaccgcggc gagccgatgg
     3061 ggacgttgtg ccccttgtcc gccaaatctg ccgggaggag actcgggtga taccgatctt
     3121 gtgggggcca tgatgatgtc gactaccacc cgttcgaatc gaaccgctcg catctcgttg
     3181 gtggcggtgg tgctcgtcgt cggcgcgctc atggccggat gcgtggtgac cccgccggcg
     3241 aaccagacgt cacgtgcgtg cgttccctcg ggggcgcttt tgccgggcca ggcggtgatc
     3301 gccatctcct cggcatcgga agggtcgtcg cctcgggtgc tgagcggc
//
LOCUS       DAOOMA010000267         3336 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_34619_length_3336_cov_3.865285, whole genome shotgun sequence.
ACCESSION   DAOOMA010000267 DAOOMA010000000
VERSION     DAOOMA010000267.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 3336)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3336)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3336
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34619_length_3336_cov_3.865285"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..552)
                     /locus_tag="PLP95_07965"
     CDS             complement(<1..552)
                     /locus_tag="PLP95_07965"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HPB45772.1"
                     /translation="MARFEPHPTRRPVLVTGASAGIGAATARAFAAIGHPVAIAARRL
                     DRLDELAAAIRSDGGEAVGVAMDVGDLDSIDAGVRAAEAAIGEIDIVVSNAGDNEPST
                     VHSDDAARFARHYDVNVFGPQRLTSVIGSAMIERGRGDIVYVSSMNASVPRVMSAAYN
                     SSKAALEAYARTAQMEFEGTGVRA"
     gene            complement(552..1745)
                     /locus_tag="PLP95_07970"
     CDS             complement(552..1745)
                     /locus_tag="PLP95_07970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020378058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HPB45773.1"
                     /translation="MTVHYSPYDYESHENPYPIYARLRSEAPLYRNDDLNFYALSRHE
                     DVGAGFRDSARFSSADGVSLDKEATGPHAYKTMSFLAMDPPRHTRMRALVARGFTPRR
                     VVELEGQIREIAIDYLDGARDGEPYDIIKDFAGRMPMDVISEMMGVPKADRDEIRRLA
                     DLLVHREEGVEGVPAAGIEAAMTLFGYYDDMVAQRRKTRTDDLTSALIDAEIDDEKLA
                     HDEIVAFLFLMVVAGNETTTKLLGNALYWAWRNPDQRDAVWSGQRSVEEWIEETLRFD
                     TSSQVLARTTTEDVEIAGGVIPAGSRVLLLAGSANHDETVFDAPEEYRIGRDTSQILS
                     FGVGRHFCMGASLARLEAKVALEEFVARVAGYELDPSGIERVHSVNVRGFSALPSTLA
                     LRAAS"
     gene            complement(1742..2320)
                     /locus_tag="PLP95_07975"
     CDS             complement(1742..2320)
                     /locus_tag="PLP95_07975"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HPB45774.1"
                     /translation="MSSSAVAARSERPSPRQVETINRLVDAAVDEIRTVGFAGLTVRN
                     VAARAGVAAATAYTYFDSKEHLVSEVFWRRLKELPLPQSNDDRPAAQRVADALRSVSN
                     LVADEPELSAAVTTALLAHDPQVKRLRDRIGANIRIRLTNSLGADDLLVVHSLELLYA
                     GALLQAGTGHLSYDELGDRLIESATLILGGRT"
     gene            complement(2463..3281)
                     /locus_tag="PLP95_07980"
     CDS             complement(2463..3281)
                     /locus_tag="PLP95_07980"
                     /inference="COORDINATES: protein motif:HMM:NF025008.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HPB45775.1"
                     /translation="MYLDASAVTRLGPTPHEEQPDSTRCRATIHDGWDILGNTHGGYL
                     LAIVGRSIAEVTGRPDPMTITAHYLSPGRPGPVSIDVETVKEGKRFSTARATMLNADS
                     KPVITALATYGDLSEGGEFPTWIDGAAPDVLPRQECVEMGAQETTPEFMRRVDLLIDP
                     RDLGFVFGERTGMPRYRGWFRLGDGELLTTDALLVAIDAFPPTIFNLDLPMGWAPTVE
                     LTGHIRAVPVDDWLCCEVRTRFVSTGFMEVDGWYWDAAGTLVAQSRQLALLPRG"
BASE COUNT          555 a         1088 c         1143 g          550 t
ORIGIN      
        1 ggcccgcacg cccgttccct cgaattccat ctgcgcggtg cgggcgtagg cctcgagcgc
       61 agccttcgag gagttgtagg cggccgacat gacccgcggg accgacgcgt tcatcgacga
      121 cacgtacacg atgtcgccgc gacctcgctc gatcatggcc gagccaatca cagaggtgag
      181 gcgctgtgga ccgaagacgt tgacgtcgta gtgcctggcg aaacgtgcgg cgtcatcgct
      241 gtgtacggtc gacggctcgt tgtcgccggc gttggacacg acgatgtcga tctcgccgat
      301 cgccgcctcg gcggctctga cgccggcgtc gatggaatcg aggtctccga cgtccatcgc
      361 gacgccgacc gcctcaccgc cgtcggagcg gatcgcggcg gcgagttcgt cgagacggtc
      421 gaggcgccgc gccgcgatcg ccaccgggtg cccgatcgcc gcaaaggcgc gggcggtcgc
      481 cgcaccgatg ccggccgagg cccccgtgac caggaccgga cggcgggtcg ggtgcggttc
      541 gaaccgtgcc atcaggaggc ggcccgcagc gccaaggtgg atggaagcgc cgagaacccg
      601 cgcacgttga ccgagtgcac ccgttcgata cccgatgggt ccagctcgta gccggcgacc
      661 ctggcgacga attcttcgag cgcgaccttg gcttccagcc gggcgagcga ggcgcccatg
      721 cagaagtgcc gtccgacccc gaagctcagg atctggctgg tgtcgcgccc gatgcgatac
      781 tcctcggggg catcgaacac cgtctcgtcg tgattggccg agcccgccag caacagcacc
      841 cgggaaccgg ccgggatgac accgccggcg atctcgacat cctcggtcgt ggtgcgtgcc
      901 agcacctgcg aggaggtgtc gaaacgcaac gtttcctcga tccactcctc gacgctgcgc
      961 tgccccgacc acacggcgtc gcgttgatcc gggttgcgcc aagcccagta gagcgcgttg
     1021 cccaacagtt tggtcgtggt ctcgttgccg gcgacgacca tcaggaacag gaaggcgacg
     1081 atctcgtcgt gggcgagctt ctcgtcgtcg atctcggcgt cgatcaacgc actcgtcagg
     1141 tcatcggtac gcgtcttgcg ccgctgggcg accatgtcgt cgtagtaacc gaacagcgtc
     1201 atcgccgcct cgattccggc tgcgggcacc ccctcgaccc cttcctcgcg atgcacgagc
     1261 aggtcggcga ggcggcggat ctcgtcgcgg tccgccttcg gcacccccat catctcgctg
     1321 atgacgtcca tcggcatgcg cccggcgaag tctttgatga tgtcgtaggg ctcgccgtcg
     1381 cgtgcgccgt cgaggtagtc gatcgcgatc tcgcggatct gaccttccaa ctcgacgact
     1441 cgccgggggg tgaatccgcg ggcgaccagg gcgcgcattc gcgtatgacg tggcggatcc
     1501 atggccagaa acgacatggt cttgtaggcg tgggggcccg tggcctcctt gtcgagggaa
     1561 acgccgtcgg cacttgagaa ccgtgccgaa tcacgaaagc ccgcgcccac gtcctcgtgg
     1621 cgcgacagcg cgtagaagtt gaggtcgtcg ttgcgataga gcggcgcctc ggagcgaagt
     1681 cgggcgtaga ttgggtacgg gttctcgtgc gactcgtagt cgtacgggct gtagtgaacg
     1741 gtcatgtgcg tccccccaag atgagtgttg cggattcgat gaggcggtcg ccgagctcgt
     1801 cgtacgacag gtggccggtg ccggcctgga gcagggctcc ggcgtagagc agttcgagcg
     1861 agtggacgac caacagatcg tccgcgccga gcgagttggt gagtcggatg cggatgttgg
     1921 cgccgatgcg gtcgcgcagt cgtttcacct gcggatcatg ggccagcagt gcggtggtca
     1981 ccgccgcgga gagctcgggc tcgtcggcca ccaggttcga caccgaccgc agagcgtcgg
     2041 cgacgcgttg agcggccggg cggtcgtcgt tggactgtgg gagcggcaac tccttgaggc
     2101 gacgccagaa cacctccgac acgaggtgct ccttggaatc gaagtaggtg taggccgtcg
     2161 cggccgccac ccccgcccgg gcggcgacgt tgcgcaccgt cagcccggcg aagccgaccg
     2221 ttcggatctc gtcgacagcc gcgtcgacca gacgattgat cgtctcgacc tgacggggag
     2281 acgggcgctc ggagcgtgcg gcgactgctg aactggacat gtgtccagga cactactgga
     2341 cacctgtcca gttttcaacc agcaatagcg gttgcgccaa acgcatcgcg acacaggact
     2401 gggcgcgcta gcgggtcgga tgtgacccgc tggcgcgccc agtccccgcg agaccgcccg
     2461 cttcaacccc gtggcaacaa tgccaactgg cgcgactggg ccaccaacgt gcccgcagcg
     2521 tcccaatacc agccatcgac ctccatgaat ccggtactga cgaatcgggt ccgtacctca
     2581 caacagagcc agtcgtcgac gggcacggca cggatgtggc cggtgagttc caccgtcggc
     2641 gcccacccca tcggcaggtc gagattgaag atggtcggag ggaaggcgtc gatggccacg
     2701 agcagtgcgt cggtggtcaa cagctcgccg tcgccgagac ggaaccaccc gcgatagcgc
     2761 ggcataccgg tgcgttcacc gaacacgaaa ccgaggtcgc gagggtcgat caggaggtcg
     2821 acccgacgca tgaattcggg cgtcgtttcc tgggccccca tctcaacgca ctcctgtcgt
     2881 ggcaacacgt cgggtgcggc gccgtcgatc caggtcggaa actcgccgcc ctcggacaga
     2941 tcgccgtagg tggcgagcgc ggtgatgacc ggcttggagt cggcgttgag catcgtcgcc
     3001 cgtgccgtcg agaaacgctt cccctccttg acggtttcga catcgatgct gacgggaccc
     3061 ggccgtccag gcgacaggta gtgcgcggtg atcgtcatcg gatccggccg cccggtgacc
     3121 tcggcgatcg atcggcccac gattgcgagc aggtagccgc cgtgggtgtt gccgaggatg
     3181 tcccacccgt cgtggatggt ggcgcggcaa cgggtcgagt cgggttgttc ttcgtgtgga
     3241 gtcggcccga ggcgggtcac cgctgaagca tcgaggtaca tcgggccact cgaccacagc
     3301 cgactcgacg ctgtccaacg tcagcggtca gcgacg
//
LOCUS       DAOOMA010000268         3335 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_34642_length_3335_cov_3.384146, whole genome shotgun sequence.
ACCESSION   DAOOMA010000268 DAOOMA010000000
VERSION     DAOOMA010000268.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 3335)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3335)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3335
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34642_length_3335_cov_3.384146"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..690)
                     /locus_tag="PLP95_07985"
     CDS             complement(<1..690)
                     /locus_tag="PLP95_07985"
                     /inference="COORDINATES: protein motif:HMM:NF023403.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB45776.1"
                     /translation="MTETTLVVDGSNIATEGRSVPSLQQLDEAVTAFIAERPHDVVIV
                     VVDATFGHRIDPSELPRFEEAVLDGELVTPPAGAIGRGDAFILQIADRAGANVFSNDS
                     FQEFHGTYDWLFEPGRLVGGKPVPPIGWVFIDRTPVRGPTSRRAVRESKQKAAKKSAA
                     SNESSRRRQASPDASGPPPVPTTPPPGQRRAAKTSAAKTSAAKTSAAKTGATKSTTRT
                     KSDSTASATATT"
     gene            758..1429
                     /locus_tag="PLP95_07990"
     CDS             758..1429
                     /locus_tag="PLP95_07990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HPB45777.1"
                     /translation="MDFDAVFFDFGGVVLSSPFEAFSVYEQRAGLPPNAVRTINATNP
                     DANAWAQFERNELSVEEFIERFEAEGRELGFDIDGVEVLRCLDGELRPEMVEVVGRCQ
                     QIATTAMLTNNVVSMSAAGFDERSGGSGIGTDGVDMFTEVLGYFDHIIESSAVGVRKP
                     ERRFYEIACEVAQVEPSRVVFLDDLGINLKPARELGMTTIKVVDPAMAIDDLEAVMGV
                     SLRVL"
     gene            complement(1490..>3335)
                     /locus_tag="PLP95_07995"
     CDS             complement(1490..>3335)
                     /locus_tag="PLP95_07995"
                     /EC_number="4.1.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020378174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004591 - oxoglutarate dehydrogenase
                     (succinyl-transferring) activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="multifunctional oxoglutarate
                     decarboxylase/oxoglutarate dehydrogenase thiamine
                     pyrophosphate-binding subunit/dihydrolipoyllysine-residue
                     succinyltransferase subunit"
                     /protein_id="HPB45778.1"
                     /translation="PIVEGMARAKMDAIPEGEGAVRFPVLPILLHGDAAFAGQGVVAE
                     TLNLSLIKGYKVGGTIHVIINNQLGFTTDPSAARSSEYCTDVAKTVQAPIFHVNGDDP
                     EACVRVARLALRYRQQFNKDVVIDMVCYRRHGHNEGDDPSYTQPLMYRSIDARRSVRK
                     VYTESLVRRGDISIEEAEVFLADFNERLQSALEETRQSAPVGEVIAKPHPPAKGVLPA
                     AETAVSAEMLDRVYEALSSHPDGFTVHPKLAKQFETRTKMYRDGEVDWALGEAFAFGT
                     TLLEGTSIRLTGQDSRRGTFSHRHSTLVDNHNGDEFVPLSLLTSDTTKLWIYDSLLSE
                     YAVLGFEYGYSVTNPAALVMWEAQFGDFMNGAQIVIDQYITSAEDKWNQTSGLVMLLP
                     HGYEGQGPEHSSARIERFMSLAAEDNIQIANPTTAAQFFHLLRRQMHREVRKPLIVFT
                     PKSGIRAKNYRSPVSDFTAGTFRELIGDASPIDPNSVTRLVLASGKVGHEAAARRDAL
                     GANAAVARVEQLFPWPYAAIKEELARYPNCTEIVWLQEEPENMGPWNGIKGRLFEAHG
                     NQYEIRRISRSEGGSPATGKAAIHAQEQHEILDKAFAPLAQRELSQFH"
BASE COUNT          549 a          971 c         1165 g          650 t
ORIGIN      
        1 agtggttgcc gtcgccgacg ccgtgctgtc ggacttggtc ctggtcgtgg acttggtggc
       61 gccggtcttg gcggcgctgg tcttggcggc gctggtcttg gcggcgctgg tcttggcagc
      121 gcggcgttgg ccgggcgggg gagtggtcgg taccggaggc ggtccactcg catccggcga
      181 tgcctggcga cgtcgcgacg actcatttga agccgcggac ttcttggccg ccttctgctt
      241 cgactcgcga accgctcgcc ggctcgtcgg cccgcgcacg ggggttcggt cgatgaagac
      301 ccaaccgatc ggcggcaccg gctttccgcc caccaggcga ccgggttcga aaagccagtc
      361 gtaggtgccg tggaactcct ggaacgaatc gttggagaac acgttggcac cggcacgatc
      421 cgcgatctgc aggatgaacg cgtcgccgcg accgatggcc ccggccggcg gggtgacgag
      481 ttcgccgtcg aggacggcct cttcgaaacg cgggagttcg gaaggatcga tccggtgacc
      541 gaacgtcgcg tcgacgacca cgatgacgac atcgtggggc cgttcggcga tgaaggcggt
      601 gacggcttcg tcgagttgtt ggaggctggg gacggatcgg ccttcggtgg cgatgttcga
      661 tccgtcaaca acgagtgtgg tctcggtcat gacaggtact aagtgaagca ggtcgtgaag
      721 tgaatactgc ggaccgtcgc gacctagggt gcaggcggtg gatttcgacg cagtgttttt
      781 cgacttcggc ggtgtcgtgc tttcctctcc gtttgaagcg ttctcagtct atgagcaacg
      841 cgctggtctt ccaccgaatg cggtcagaac gatcaacgcc accaaccccg atgcgaacgc
      901 ctgggcgcag tttgaacgca acgagttgag tgtcgaggag ttcatcgagc gctttgaagc
      961 ggagggtcgg gagctgggtt tcgacatcga cggtgttgaa gtgttgcgct gtctcgacgg
     1021 agagctgcgc ccggagatgg tggaggtcgt ggggcggtgc cagcagattg cgacgacggc
     1081 gatgttgacc aacaacgtcg tgtcgatgag cgcggccggc ttcgacgagc ggtccggtgg
     1141 ttccggtatc ggcactgatg gtgttgatat gttcaccgaa gtgctcggat acttcgacca
     1201 catcatcgag agctcggcgg tgggggtgcg aaagccggaa cggcgtttct atgagatcgc
     1261 gtgcgaggtc gcgcaggtgg agccctcgag ggtcgtgttt ctcgacgacc tcggaatcaa
     1321 tctcaagccg gcgcgcgaac tcggcatgac gacgatcaag gtcgtcgacc ccgcaatggc
     1381 gatcgacgac cttgaagcgg tgatgggcgt ttcgttgcga gttctgtagg taatccatgt
     1441 cgctatggcg acgggaatta cctacagaac ggcggggcgt ggaaccggct cagtggaact
     1501 ggctcagttc gcgctgagcg agcggagcga aggccttgtc gaggatctcg tgttgttcct
     1561 gggcgtggat cgccgccttg ccagtcgccg gtgatccgcc ctcggagcgg ctgatgcggc
     1621 ggatctcgta ctggttgccg tgagcctcga agagccggcc cttgatcccg ttccaggggc
     1681 ccatgttctc gggttcctcc tgcagccaca cgatctcggt gcagttgggg tagcgggcaa
     1741 gctcctcctt gatggcggca tagggccaag ggaagagttg ctcgacgcgc gcgaccgcgg
     1801 cgttcgcccc gagcgcgtcg cgccgtgctg ccgcctcgtg gccgaccttg ccgctcgcga
     1861 gcaccaaacg ggtgaccgag ttcgggtcga tcggcgacgc gtcgccgatc agctcgcgga
     1921 acgtgcccgc ggtgaaatca gaaaccgggc tgcgatagtt cttcgcgcgg atgcccgact
     1981 tcggggtgaa cacgatgagc ggcttgcgca cctcgcggtg catctggcga cgcagcaggt
     2041 ggaagaactg tgcggccgtc gtcgggttgg cgatctgaat gttgtcctcg gcagccaggg
     2101 acatgaaccg ttcgatacgg gccgaggagt gctccggtcc ctgcccctcg tagccgtggg
     2161 gcaacaacat gaccaacccg gaggtctggt tccacttgtc ttctgcggag gtgatgtatt
     2221 gatcgatgac gatctgtgcc ccgttcatga agtcgccgaa ctgggcctcc cacatgacca
     2281 gcgccgccgg gttggtcacc gagtacccgt actcgaagcc caacaccgcg tactcgctga
     2341 gcagggagtc gtagatccac agcttggtcg tgtcggaggt gagcagcgac agcggaacga
     2401 actcgtcgcc gttgtggttg tcgacgaggg tggagtggcg gtggctgaaa gtgccgcggc
     2461 ggctgtcctg gccggtcaga cgaatcgagg ttccctcgag cagtgtcgtg ccgaacgcga
     2521 atgcttctcc gagggcccaa tcgacctcac cgtcgcgata catcttggtt cgggtctcaa
     2581 attgcttggc cagcttcggg tgaaccgtga atccatcggg atgtgaggac aacgcctcgt
     2641 agacgcggtc gagcatctcc gccgacacag cggtctccgc cgcaggcagc acccccttgg
     2701 ccggtgggtg gggcttggcg atcacctcgc cgaccggtgc ggactgacgt gtttcttcga
     2761 gtgccgactg caggcgctcg ttgaagtcgg ccaggaagac ctcggcctcc tcgatcgaga
     2821 tatcgccgcg gcggaccagg ctttcggtgt acaccttgcg cacgctgcga cgggcgtcga
     2881 tcgagcgata catgagcggc tgggtgtagc tcgggtcgtc gccctcgttg tggccgtggc
     2941 gccgatagca caccatgtcg atcacgacgt ccttgttgaa ctgctggcga taccgcaacg
     3001 ccaagcgcgc cacgcgcacg caggcctcgg ggtcgtcgcc gttgacgtgg aagatcggcg
     3061 cctgaacggt cttggccacg tcggtgcagt attccgaact gcgcgcagcc gacgggtcgg
     3121 tggtgaagcc gagttgattg ttgatgatga cgtggatcgt gccgccgacc ttgtaaccct
     3181 tgatgaggga gagattcagg gtttcggcca ccacaccctg gccggcaaac gcggcgtcgc
     3241 cgtgcaacag gatcggcaac accgggaagc gcaccgctcc ctcgccctcg gggatggcgt
     3301 ccatcttggc gcgggccatg ccctccacga tcggg
//
LOCUS       DAOOMA010000269         3329 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_34727_length_3329_cov_3.633781, whole genome shotgun sequence.
ACCESSION   DAOOMA010000269 DAOOMA010000000
VERSION     DAOOMA010000269.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 3329)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3329)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3329
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34727_length_3329_cov_3.633781"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..532)
                     /locus_tag="PLP95_08000"
     CDS             complement(<1..532)
                     /locus_tag="PLP95_08000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HPB45779.1"
                     /translation="MNQRDAQKLETRRQLSAAAIELFAERGYDDTRIDDIAARVGVSS
                     RTFFHHFPTKEFAVFPDHNERVEALTALLAVTPPDRDPFDIVFEQVRFGLTSTISSPM
                     RTERYRLISRVDALRERDALNDLDYEAALSEYLVRAWSGSGDATLIAFRARLVAGSAM
                     AIARAALTAWIGDPSFD"
     gene            585..1982
                     /locus_tag="PLP95_08005"
     CDS             585..1982
                     /locus_tag="PLP95_08005"
                     /inference="COORDINATES: protein motif:HMM:NF012503.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HPB45780.1"
                     /translation="MSDADDFLNSIYPYRTRFETHAVLPAHGVDRATILEQLGAMAQE
                     EDAKGDRGQVSGSLYHGGHDHYHFLTEAFEAFAHANVIQRDMYPSATKMEGEIVAMAL
                     DMFGGGHAEDACGVITSGGSESLMTAVYTYREAGRERGITEPEMIWPETAHVALDKGA
                     YYFGVKVIKAPVDENYQVDVAWVRDHITPNTVAIAASAGTYPHGVIDPITELGALALE
                     KGIGFHVDGCLGGFILAWAKDCGVEVPPFDFRVPGVTSMSADTHKYGYALKGTSVLMY
                     SSKELRKHQYFTVGGWPGGIYISPGMAGSRSGGVIAATWAAMLNLGREGYVEIAKGIF
                     EAADKLIAGIEAIPELQILGKPYFLVAFASADPDLNIYHVNDALAARGWRMNGLQKPD
                     AIHFCVTRPNTAPGLIEQFLEDLRASVDEAKGKEGEPDSGALYGLSGTGPEGVAVVED
                     ILAGALDAFYDVPKG"
     gene            1988..>3329
                     /locus_tag="PLP95_08010"
     CDS             1988..>3329
                     /locus_tag="PLP95_08010"
                     /inference="COORDINATES: protein motif:HMM:NF012589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY family carbohydrate kinase"
                     /protein_id="HPB45781.1"
                     /translation="MAAASDPRAERFVLTVDLGTGGPKVGFVSLLGSVAFEEHYVAET
                     EFGADGLASQDAETWWRTIIDAAKGALDAGVIRRDQVAAVSITGQWASTVCVDAEGLP
                     TSPVMMWMDNRGGRLAREAVGGPVSGYAPRAIAEFIRRSGGAPSLDGADPIGHRLYLQ
                     RCEPERYRAARWLMEPVDYLSMRFTGIAAASPASMVGTWMLDTRDPKNYRYDDTLLAL
                     TGGGREKLPPLQPFGTVIANVQPSVAAELGIGADVAVVTGAPDVHTAAIGSGAIGLYE
                     THMAVSTTGWISAPVPKKKTDLFNMVATVPGIDEENYLIINNHETSGACFAWLRGILG
                     GTLDFEEMNDLAGESPAGANGVIFTPWLKGERSPIADANARAGFANLGLLNTRSDLIR
                     AVMEGVAINDRWLHDAVEKFAGRTLDNIRVIGGGAQSDLWCQIHADVMGRTIEQV"
BASE COUNT          575 a         1078 c         1121 g          555 t
ORIGIN      
        1 ggtcgaagct ggggtctcca atccaggccg tgagcgccgc ccgggcgatg gccatggcgc
       61 tgccggcgac cagccgggcc cggaacgcaa tcagtgtggc gtcgccggat ccgctccagg
      121 ctcggacgag gtactcgctc agtgccgcct cgtagtcgag gtcgttgaga gcgtcgcgtt
      181 cgcggagcgc gtcgacccgg gagatgaggc ggtagcgttc ggtgcgcatg ggcgatgaga
      241 tcgtcgaggt caacccgaaa cggacctgtt cgaacacgat gtcgaacgga tctcgatccg
      301 gcggggtgac ggcgagcagc gcggtgaggg cctcgacgcg ttcgttgtgg tcgggaaaca
      361 ccgcgaattc cttggtgggg aagtggtgga agaacgtgcg cgaactcacc ccgacccgag
      421 cggcgatgtc gtcgatccgg gtgtcgtcgt agccgcgttc cgcaaacagc tcgatggcgg
      481 ccgccgagag ttgtcggcgg gtctccagct tctgtgcgtc gcgctggttc atccactcag
      541 cttacccgtt gacttcacag agtgaagcga aatagcttga ggtcatgagt gacgccgacg
      601 acttcctgaa ttcgatctac ccgtaccgga ctcgtttcga gacccacgcc gtgctgcccg
      661 cacacggagt cgatcgcgcc acgatcctcg aacaactcgg cgccatggcc caagaggagg
      721 acgccaaggg cgaccggggc caggtctcgg gatcgctcta tcacggaggc cacgaccact
      781 atcacttcct caccgaggcg ttcgaggcct tcgcgcacgc gaacgtcatt cagcgcgaca
      841 tgtacccgag cgccacgaag atggaaggcg agatcgtcgc gatggcgctc gacatgttcg
      901 ggggcggcca cgctgaggat gcctgcggtg tcatcacctc gggcggttcg gaatcgttga
      961 tgaccgcggt ctacacctac cgcgaggccg gacgggagcg cggcatcacc gaacccgaga
     1021 tgatctggcc cgaaaccgcg cacgtcgccc tcgataaggg tgcgtactac ttcggtgtga
     1081 aggtcatcaa ggccccggtc gacgagaact atcaggtcga cgtcgcctgg gttcgcgacc
     1141 acatcacccc caacaccgtg gccatcgcgg cctctgccgg cacctacccg cacggggtca
     1201 tcgacccgat caccgaactc ggtgcactcg cactcgaaaa gggcatcggc tttcacgtcg
     1261 acggctgtct cggcgggttc atcctggcgt gggcgaagga ctgcggggtc gaggttcccc
     1321 cgttcgattt ccgggttccc ggagtcacct cgatgtcggc cgacacccac aagtacggct
     1381 acgcgctcaa gggcaccagc gtgttgatgt attcctcgaa ggaactgcgc aagcaccagt
     1441 acttcaccgt cggcggctgg ccgggcggca tctacatctc cccgggaatg gccggtagcc
     1501 gctcgggcgg agtcatcgcc gcgacctggg cggccatgtt gaacctgggc cgcgagggct
     1561 atgtcgagat cgccaagggg atcttcgaag ccgccgacaa gctgatcgcc ggcatcgagg
     1621 ccatccccga gttgcagatt ctcggcaagc cctacttcct tgtggcgttc gcctcggcgg
     1681 atccggatct gaacatctac cacgtcaacg atgctctggc cgcccgcggt tggcgcatga
     1741 acggcctgca aaagcccgac gccatccact tctgcgtcac ccggcccaac accgcaccgg
     1801 gcctgattga acagttcctc gaggacctcc gagcatcggt ggacgaggcg aagggcaagg
     1861 agggagagcc cgattcgggt gcgctctacg gcctgtccgg aaccggcccc gagggcgtcg
     1921 cggtggtcga ggacatcctc gccggcgcac tcgatgcgtt ctacgacgtt ccgaagggct
     1981 gaggtcgatg gccgctgcgt ctgacccccg tgccgagcgt ttcgtcctca ccgtcgacct
     2041 cggcaccggg gggcccaagg tcgggttcgt gtcgctgctt ggcagcgttg ccttcgagga
     2101 gcactacgtt gccgagaccg agttcggcgc ggacggattg gcatcccagg acgccgagac
     2161 gtggtggcga acgatcatcg atgcggccaa gggcgctctc gatgccgggg tcatccgccg
     2221 cgatcaggtc gcggcggtct cgatcaccgg acagtgggcg tcgaccgtgt gcgtcgatgc
     2281 cgagggactg ccgacctccc cggtgatgat gtggatggac aatcggggcg gccgcctcgc
     2341 acgcgaggcg gttggaggcc cggtcagcgg ctacgcgccg agggcgatcg ccgagttcat
     2401 ccggcgatcc ggtggcgccc ccagcctcga tggcgccgat cccatcgggc atcgcctgta
     2461 tctgcagcgc tgcgaacccg agcggtaccg ggccgcgcgc tggctgatgg aacccgtcga
     2521 ctacctgtcg atgcgtttca cgggcatcgc cgctgcgagc ccggcgtcga tggtcggtac
     2581 gtggatgctc gacacccgcg atccgaagaa ctaccgctac gacgacacgc tgttggcgct
     2641 gacgggcggc gggcgcgaga agctgccacc ccttcagccg ttcggcaccg tcatcgccaa
     2701 cgtgcagccg tcggtcgccg ccgagttggg catcggcgcc gacgtcgcgg tggtgaccgg
     2761 tgccccggac gtgcacaccg cggcgatcgg ctcgggggcc atcgggctct atgagaccca
     2821 tatggcggtg agcacgaccg gatggatcag cgcacccgtg ccgaagaaga agaccgacct
     2881 gttcaacatg gtcgcgaccg tgcccggcat cgacgaggag aactacctca tcatcaacaa
     2941 ccacgagacc tccggagcgt gctttgcgtg gttgcgtggg atcctcggcg gcacgctcga
     3001 tttcgaggag atgaacgacc ttgcgggcga gtcgccggcg ggcgccaacg gggtgatctt
     3061 cacaccgtgg ctgaagggag aacgatcgcc gatcgccgac gccaacgccc gtgcgggttt
     3121 tgccaacctc ggcctcctca acacccgctc cgacctcatc cgcgcggtga tggagggcgt
     3181 cgcgatcaac gaccgatggt tgcacgacgc cgtcgagaag ttcgccgggc gaaccctcga
     3241 caacatccgt gtgatcggtg gaggggcgca gtcggacctg tggtgtcaga tccacgcgga
     3301 cgtgatgggc cgcacgatcg aacaggttg
//
LOCUS       DAOOMA010000270         3329 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_34728_length_3329_cov_3.380880, whole genome shotgun sequence.
ACCESSION   DAOOMA010000270 DAOOMA010000000
VERSION     DAOOMA010000270.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 3329)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3329)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3329
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34728_length_3329_cov_3.380880"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(1..891)
                     /locus_tag="PLP95_08015"
     CDS             complement(1..891)
                     /locus_tag="PLP95_08015"
                     /inference="COORDINATES: protein motif:HMM:NF012770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HPB45782.1"
                     /translation="MSITAPRYEGRVQVRDGRSLGIAEFGPPHAEQTVLWFHGTPGAR
                     KQIPDNARRLAFERGIRVIGIDRPGVGLSTAHRYHRLLDFTDDLEVAIDRLGVERFSV
                     VGLSGGAPYALAAAHAFADRVPTVGVLSGVVPSGGADGTDGGLVGIAVRFKPLLPVLS
                     EPFGAVMTNFVRVAKPVAPFALDMFARISPPGDREIFAVPEHREMFLDDVLTGGRHGM
                     KAAGYDAVLFTRYWGFSVRDVATRVVWWQGDDDYIVPLSHAEHIVPLLPHGELRVQPG
                     GGHLCGLGLGDEVLETILDW"
     gene            complement(960..1853)
                     /locus_tag="PLP95_08020"
     CDS             complement(960..1853)
                     /locus_tag="PLP95_08020"
                     /inference="COORDINATES: protein motif:HMM:NF023015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diiron oxygenase"
                     /protein_id="HPB45783.1"
                     /translation="MSTVTTTERIQRLNRVSEKRVVNPDTDLIGSLRDGQILPDELLS
                     IAGLDLDLSAEQRATLSREEVASLFDNGIRFEAILEAGFALEIAAARDLTDPRVTYLC
                     HEIGEETRHQRIFQRMIQQIRPTAKNPLAGNRAVAVFERMSNTWLINHTMVLYVMVLA
                     GEEIPDLLQKLASEHPDTDPWLAEVNRYHRQEEARHLSFARARLPELWQSASRVDRFA
                     VRHVVPLAIAQLFEWLVHPGVYETVGLDGWATWKAVNRSPQRQAIRHRATRPILAELQ
                     SAGIIDANRVPKRWQRLCGIG"
     gene            1965..2702
                     /locus_tag="PLP95_08025"
     CDS             1965..2702
                     /locus_tag="PLP95_08025"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HPB45784.1"
                     /translation="MTEGRATPRQRAITRRKPGRPKGPSSDPAQRLDSLLDGAEDAIR
                     THGPSVSMEQLAAAAGVSKATLYDNFAGKAGLSEALMDRKGAALLETFSATLADGFTP
                     EQFVRRGITIFVEHVATDPEIYRFIVNSTEIGEAVVDDIAAPITAILDALLDPGDALD
                     ADAHNADAHDSDASLAAFLANTALGAILGSTDWWSRSKSPSVERFSSALADFVWAGLI
                     GAGLPPSDEQLDVAAVAAAIAAAEPRR"
     gene            2859..>3329
                     /locus_tag="PLP95_08030"
     CDS             2859..>3329
                     /locus_tag="PLP95_08030"
                     /inference="COORDINATES: protein motif:HMM:NF024986.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HPB45785.1"
                     /translation="MTSLVEAGVRRSTTVIALVVCLAAGACAANPTSTWVGSDCGASP
                     NLGPGASLFGCNLTGLDLSNLNLSGADLRGAVLVNANLAGSDLSGANLEGANLTGANL
                     TGANLTGAILAGAILLGALFIGANLAGVNFDFGLVYGASGNGGGRPSGNSCVGAY"
BASE COUNT          580 a         1139 c         1094 g          516 t
ORIGIN      
        1 ctaccagtcc aggatcgtct cgagcacctc gtcacccaag ccgagcccgc acaagtgccc
       61 accacccggc tgcacccgca actcgccatg cggaagcaac ggaacgatgt gttcggcgtg
      121 tgacagcggc acgatgtagt cgtcatcgcc ttgccaccac acgaccctcg tcgcaacgtc
      181 gcgaaccgaa aagccccagt agcgggtgaa caacacggcg tcgtagcccg ccgctttcat
      241 cccgtgacga ccgccggtga gcacgtcgtc gaggaacatc tcacggtgct ccggcaccgc
      301 gaagatttcg cggtcaccgg gaggggaaat gcgggcgaac atgtcgagcg cgaacggagc
      361 gaccggcttg gccacccgca cgaaattcgt catcaccgcg ccgaacggtt cgctcaggac
      421 cggcaacagc ggcttgaacc ggaccgcgat accaaccaga ccgccgtcgg tgccgtcggc
      481 tcccccgctc gggaccacac cgctgagaac cccgaccgtc ggcacccgat cggcaaacgc
      541 gtgcgccgca gccaacgcgt agggggcacc accggacagg ccgacgaccg aaaagcgctc
      601 cacccccaaa cggtcgatcg cgacctccag gtcgtcggtg aagtcgagaa gccggtggta
      661 ccgatgcgcc gtcgacagcc cgacccctgg ccgatcgata ccgatgacgc ggatacctcg
      721 ctcgaacgcg agccttcgag cattgtcggg gatctgcttg cgtgccccag gggttccgtg
      781 aaaccacaac accgtctgtt cggcgtgcgg cgggccgaac tcggcgattc cgagcgagcg
      841 gccatcgcgc acctggacac ggccctcata tcgcggcgcg gtaatagaca tcgccccatc
      901 gtgacagatg acacgcccag attcgactcc gacccggccg gcggccaggg gcgggaagct
      961 cagccgattc cgcagagtcg ctgccagcgc ttgggaaccc tgttcgcgtc gatgatgccg
     1021 gcggactgga gctccgccag gatcgggcgg gtggcgcggt ggcgaatcgc ttgacgctgc
     1081 ggcgagcggt tgacggcctt ccatgtcgcc cagccgtcca gcccgaccgt ttcgtacacc
     1141 cccgggtgaa ccagccattc gaacagctgg gcgatggcga gcggcacgac gtggcggacg
     1201 gcaaagcggt cgacacgcga ggcgctctgc cacaactccg gcagccgcgc ccgggcgaac
     1261 gagaggtggc gggcctcttc ttggcggtga taccggttga cctcggcgag ccacgggtcg
     1321 gtgtcggggt gttcgctggc gagcttctgc aacaggtcgg ggatctcctc gcccgcgagc
     1381 accatcacat agagcaccat cgtgtggttg atgagccacg tgttcgacat ccgttcgaac
     1441 accgccaccg cacggttgcc ggcaagcggg ttcttcgccg tcggccggat ctgttggatc
     1501 atgcgctgga agatccgttg gtgtcgcgtc tcctcgccga tctcatggca cagataggtg
     1561 acccgcgggt cggtgaggtc gcgggccgcg gcgatctcga gcgcgaagcc cgcctcgagg
     1621 atggcctcga agcggatgcc gttgtcgaac agcgaggcga cctcctcgcg gctgagcgtg
     1681 gcccgctgtt cggcgctgag gtcgagatcg aggccggcga tcgagaggag ttcgtcgggg
     1741 aggatctggc cgtcgcggag gctgccgatg aggtcggtgt cggggttgac gacgcgtttc
     1801 tcgctgacgc ggttcagccg ttggatgcgt tcggtggtgg tgacggtcga catgggtcga
     1861 gcataaaccg acccatggtc ggtttacaac caccttctcc ttccacggcg accagacaca
     1921 cgacgcttcg cagacatttc ccgccgcggc actaggttga tcgggtgacc gagggccgag
     1981 cgacaccacg ccagcgtgcg ataacgcgcc gcaaaccagg ccgaccgaag gggccgagct
     2041 cggatccggc acagcgactc gattccctcc tcgacggggc ggaggacgca attcgaaccc
     2101 acggcccgtc ggtgtcgatg gaacaactcg ctgccgccgc cggcgtgtcg aaggccacgc
     2161 tgtatgacaa cttcgccggc aaggcggggc tgagcgaagc cctaatggac cgcaaggggg
     2221 ccgcgctcct cgagacgttc tcggcgaccc tggccgacgg gttcaccccc gaacagttcg
     2281 tgcgacgggg catcacgatc ttcgtcgaac acgtcgcaac cgaccccgag atctaccggt
     2341 tcatcgtcaa ctccaccgag atcggagaag cggtcgtcga cgacatcgcc gcgccgatca
     2401 ccgccatcct cgacgcactg ctcgaccccg gcgacgcgct cgacgccgac gcgcacaacg
     2461 ccgacgcgca cgacagcgat gcttcgctcg cagcctttct cgccaacacc gcgctcggcg
     2521 cgattctggg aagtaccgac tggtggagcc gatccaagtc gccgtcggtc gaacgcttca
     2581 gctccgccct cgccgacttc gtgtgggcgg gcctgatcgg tgccggcctt cccccgagcg
     2641 acgagcaact cgacgtcgcc gcggtcgccg ccgccatcgc cgcggccgag cccaggcgct
     2701 gaggccgcac tccccaggct caaagcgcga cggcccccag cggcgccaac caacaacaaa
     2761 tccgtacacg tgtcggggaa tgtctctaat gtgcgtcgag gggaccgagc cgatacgtcg
     2821 tgggcgatcg gctctcactc agcgatcggg gaggatcgat gacaagtttg gtggaagcgg
     2881 gcgttcgacg ttccacgacg gtgatcgcgt tggtggtctg cctggcggcc ggggcgtgcg
     2941 ctgccaaccc gacctcgacg tgggtcggta gcgactgcgg ggcgagcccg aacctcggcc
     3001 ccggcgcatc gttgttcggt tgcaatctga ccggcctcga cctgtcgaac ctcaatctgt
     3061 cgggtgccga tcttcgtggc gccgtgctcg taaacgcgaa cctcgccggt tccgacctga
     3121 gcggggcgaa ccttgagggc gccaacctga ccggggccaa cctgaccggg gccaacctca
     3181 ccggcgccat cctggccggg gccatcctgc tcggcgcgtt gttcatcggc gcgaacctgg
     3241 ccggggtgaa cttcgatttc ggcctggtct acggagcgtc cggcaacggt ggcggacgac
     3301 cgagcggtaa ctcgtgtgtc ggcgcctac
//
LOCUS       DAOOMA010000271         3320 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_34855_length_3320_cov_4.372435, whole genome shotgun sequence.
ACCESSION   DAOOMA010000271 DAOOMA010000000
VERSION     DAOOMA010000271.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 3320)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3320)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3320
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34855_length_3320_cov_4.372435"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(347..1186)
                     /locus_tag="PLP95_08035"
     CDS             complement(347..1186)
                     /locus_tag="PLP95_08035"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HPB45786.1"
                     /translation="MAQTHTLQGMSALVTGGGSGIGLASARALVRDGASVTIMGRNAE
                     KLAAAAASLREDVVDGAAVTEHSGDVGNEEDVRAAVAAACEPTGRLDMVVAAAGDGTL
                     GPVIATSLDEWNRVLNVCLTGTFLTFKHAGAVMGRNGGGSLVAISSLASSITHPFMAP
                     YSAAKAGIDMLVKTIADELGQVGVRANAVNPGIIETDLVSMITPDNSVGQSYLDAMPI
                     SRFGNVDDVAPLVRFLCGPESSFITGETITVDGGNHLRQGPNYYEFASALYGSAADGI
                     VEP"
     gene            1219..2433
                     /locus_tag="PLP95_08040"
     CDS             1219..2433
                     /locus_tag="PLP95_08040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB45787.1"
                     /translation="MTKNLRWQVMDHPQSSHHPETHSSDGADGSIDEMTTSEADDGTE
                     EAESLVHAAEERAASAARLAEALEILAAAEPVAYHKSLTALLRTGHDGLLKPMPYDAS
                     KDYHDPSCVVVDPVPEGLDTMRLYSRFAYRDFDRVLADPINDPRYDHLLTSLYHLTVN
                     EGCNVAVVTNHGELHDIALVLSALVLALCDEDRTFGVLGEHVGLDEFTDRSNLLLSRM
                     VATTEVFNIATTEVLRLMCRTFYSVPQTASRRRSKLDPDLARANNLVMRHLLEQRLEG
                     GGQILAMAASGSQDISVASGLARKIRAAFRARRGDVREDTPSLHLQPLYNGTMNLMMT
                     CDYVVPIAISLDQDHPACEIGSLTKVRTQDDCHEVMNWIASAHESATGIPTIYHQRED
                     DLLTQVRDALRS"
     gene            complement(2486..>3320)
                     /locus_tag="PLP95_08045"
     CDS             complement(2486..>3320)
                     /locus_tag="PLP95_08045"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442759.1"
                     /note="converts 2-oxoglutarate to glutamate; in
                     Escherichia coli this enzyme plays a role in glutamate
                     synthesis when the cell is under energy restriction; uses
                     NADPH; forms a homohexamer; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glutamate dehydrogenase"
                     /protein_id="HPB45788.1"
                     /translation="VGGREIGYLFGQYKRITNRYESAVLTGKGLTWGGALVRTEATGY
                     GATFFAEEMLKARGETLDDKTTVVSGSGNVAIYAIEKVHQLGGKVVAASDSAGYIYDE
                     NGIDLDLLKEIKLVERGRISTYADRRGSAKFVSGGSIWDVPCQVALPSATQNELTGQD
                     AETLVRNGVIAVSEGANMPSTPEAIEVFQAAKIAYGPGKAANAGGVATSALEMQQNAS
                     RDSWTFEYTEERLQKIMQGIHTQCIEAAEEYGDPGNYVLGANVAGFLKVADAMMAFGV
                     I"
BASE COUNT          567 a         1069 c         1064 g          620 t
ORIGIN      
        1 cccctgaccc acacacgcgg acccccaccc gaccccgctc aaatccgaag cgccaagaaa
       61 ctccgggttt gttggttaga tccggccgtt gatgcggtcg gggtagtgga tggtcagggt
      121 gttcaaaatg gttttccagc ctcggatttg gccggtgagg tcttggcggt tcggccttcg
      181 acgggtagcg atcaggtaca ggactttcat cgcggacggc tcgttgggga agtggccgcg
      241 gtgacggtgc ggaggcgggg aggatggtgt ccgagactgg ggtgaggcaa cccgactcgg
      301 cgtcctcaac tacctcggca agtggcgtgg tctgagcgtc ccgggcttag ggttcgacga
      361 tgccgtccgc cgcgctgccg tagagcgccg aggcgaactc gtagtagttc gggccctggc
      421 gcaggtgatt tccgccgtcg accgtgatcg tctcgccggt gatgaacgat gactcggggc
      481 cacacaggaa acgaaccagc ggcgcgacgt cgtcgacgtt gccgaatcgg ctgatcggca
      541 tcgcgtcgag gtaactctgc ccgaccgaat tgtcgggggt gatcatcgac acgagatcgg
      601 tttcgatgat gccggggttg accgcgttgg cccggacccc gacctggccc aattcatccg
      661 cgatcgtctt caccaacatg tcgataccgg ccttggccgc gctgtagggg gccatgaacg
      721 ggtgggtgat cgacgacgcc agcgacgaga tcgcgaccag cgagccaccg ccgttgcggc
      781 ccatgacggc ccccgcgtgc ttgaaggtga gaaacgtgcc ggtgagacag acgttgagga
      841 cacggttcca ctcgtccaac gacgtggcga tcaccggccc gagcgtgccg tcgccggccg
      901 cggccaccac catgtcgaga cgtcccgtgg gctcgcacgc ggcggcgacc gcggccctga
      961 catcctcctc gttgccgacg tcaccgctgt gctcggtgac ggccgcgccg tcgacgacgt
     1021 cctcgcgaag gctcgctgcg gcggccgcca gcttttcggc gttgcggccc atgatcgtga
     1081 ccgaggctcc atctcgaacg agcgctcgag ccgaggccaa tccaattccg cttccgccgc
     1141 cggtgacgag tgcggacatt ccttggaggg tgtgggtctg agccatgggt ggatgttatg
     1201 ggctgcgtag ctccgtcgat gacgaagaat ctacgctggc aggtcatgga tcatccccag
     1261 tcctctcacc atcccgagac ccattcgagc gatggcgccg acggctcaat cgatgagatg
     1321 acaacctcgg aggcggacga cggcaccgag gaggccgaat cgctcgtaca cgccgccgaa
     1381 gagcgtgccg cttcggccgc ccgccttgct gaggcgctgg aaattctcgc ggcagcggag
     1441 ccggtggcgt accacaagtc cctgacagca ttacttcgca cgggccacga cggcctgttg
     1501 aagccgatgc cctatgacgc gtcgaaggac tatcacgatc cgagctgcgt ggttgtcgac
     1561 cccgttcccg agggcctcga caccatgcgg ctgtacagcc gattcgcgta ccgggatttc
     1621 gaccgggtcc tggctgaccc gatcaacgat ccccgctacg accatctcct caccagcctc
     1681 taccacctca cggtcaatga gggctgcaac gttgcggtgg tgaccaatca cggagaactc
     1741 catgacatcg cactcgttct gtcagcgttg gttctggcgt tgtgcgatga ggaccggacc
     1801 ttcggagtcc tcggtgaaca cgtgggactc gacgagttca ccgatcgttc gaacttgttg
     1861 ttgagtcgta tggtggcgac caccgaagtg ttcaacatcg cgaccactga ggtgttgcgg
     1921 ctcatgtgtc ggaccttcta cagcgtccct cagacggcga gtcgtcgccg atccaaactc
     1981 gaccccgacc tggcccgcgc caacaacctc gtgatgcgtc acctgctcga gcaacgcctc
     2041 gagggaggcg ggcagatcct tgcgatggcc gcgagcggga gtcaggacat ctcggtcgcc
     2101 agcggcctcg ctcgaaagat ccgggcggca tttcgtgctc ggcggggcga cgtgcgcgag
     2161 gacacgccga gtctgcatct gcagccgctc tacaacggca cgatgaacct gatgatgacc
     2221 tgcgattacg tggtgccgat cgccatttcg ctcgaccagg atcatccggc ctgtgagatc
     2281 ggttcgctca ccaaggtgcg cacccaggac gactgccacg aggtgatgaa ctggatcgcg
     2341 tcggctcacg agtcggcgac gggaatcccc acgatctatc accaacgcga ggacgacctg
     2401 ctgacccagg tgcgcgacgc cctcaggtcg tgatgccctc aggtcgtgat gccctcaggt
     2461 cgtgatgccc accgggcggc tcgggtcaga tgacgccgaa ggccatcatg gcgtcggcaa
     2521 ccttcaagaa gccagcgacg ttggcaccga gcacgtagtt gcctgggtcg ccgtattcct
     2581 cggccgcctc gatgcactgt gtgtggatgc cctgcatgat cttttgcagc cgctcctcgg
     2641 tgtattcgaa cgtccacgaa tcgcggctgg cgttctgttg catttcgagc gctgaggtcg
     2701 cgacgccgcc ggcattggcc gccttacccg ggccgtaggc gatcttcgcc gcctggaaga
     2761 cctcgatcgc ctcgggggtt gaaggcatgt tcgcaccctc cgacacagcg atcaccccgt
     2821 ttcgcacgag cgtctcggcg tcctggcccg tcagctcgtt ctgggtcgcg cttggaaggg
     2881 caacctggca gggcacatcc cagatgctcc ctccggacac gaacttcgcc gagccgcggc
     2941 gatcggcata ggtggaaatt cggccgcgct cgacgagctt gatctccttc agcaggtcga
     3001 ggtcgatgcc gttctcgtcg tagatgtacc cggccgagtc ggaggctgcg acgaccttgc
     3061 cgccgagttg gtggaccttt tcgatggcgt agatcgcgac gttgcccgac cccgacacca
     3121 cggtggtctt gtcgtcgagg gtctcgccgc gggccttgag catctcttcg gcgaagaaag
     3181 ttgcgccgta gccggtcgcc tcggtgcgca cgagcgcgcc accccaggtc agccccttgc
     3241 cggttagcac ggccgactcg taacggttgg tgatgcgctt gtactgcccg aacaaatagc
     3301 cgatctcgcg tccacccaca
//
LOCUS       DAOOMA010000272         3305 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_35081_length_3305_cov_3.684923, whole genome shotgun sequence.
ACCESSION   DAOOMA010000272 DAOOMA010000000
VERSION     DAOOMA010000272.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 3305)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3305)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3305
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35081_length_3305_cov_3.684923"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..728
                     /locus_tag="PLP95_08050"
     CDS             <1..728
                     /locus_tag="PLP95_08050"
                     /inference="COORDINATES: protein motif:HMM:TIGR00350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HPB45789.1"
                     /translation="RRADALIVVQKRRDRASALVVSIPRDLVVFRAGVGADRLALTLL
                     DGPASTLNSLCRTLGIEIDHAAVVDFAGVADIVDGLGGVEVSAEHPSRDRATGLSLEE
                     GTTLLDGIEAVAWARSRHMEHLVDGRWVSVEGSDDNRQENQRVLLESVVARLRSRWWN
                     LLQLERVAWSGSQAVTLSASTELIDVARLGASLGSGYDTIELHHSLDDGDIPVARLQG
                     DANAVLNELNAGSTSCPVPDFVQ"
     gene            complement(682..>3305)
                     /locus_tag="PLP95_08055"
     CDS             complement(682..>3305)
                     /locus_tag="PLP95_08055"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HPB45790.1"
                     /translation="LLRPPGPQRAPAFDGRRTGGAAHVSTSSTRLLRRHLVTQRSLTV
                     PLAVIITQVSQFGPHRLATAAAAVAIGAAEIILFWHLAARHPRSLALLTFLDFAAVAA
                     VGVVVPQLFQWGVCITVVSIPGQHIHHGRRIATWSLLGGTAFYALVGLGAGLPNWAPA
                     VALFATAGFSTTTLIAQSADEALRVRTRFTDLLDRLDIIAFDSPAGSPNFRFFSDRIE
                     QVLGYSTDEWTRRTTWLERLHPADVEAFLSTLETGTPGTSHLTTLRVRGQDERTVYLL
                     AQVVWREEGLWGIFIDVTRNHAQEREMAALGDFLTQIPYALEIVKVDHSGEVPEFELI
                     SASKAAEEAIGLPVTESTEGWPEASRFVDTWPQHLAAFAEVDRTQTPMQPSEVRWSDG
                     AGTERWITVQAFPIARHHIGVVCDDVTDRVQAADALRVQALHDSLTGLANRTMFGNAI
                     SEVLTTVNDVSGAVLLMDLDQFKEVNDALGHHHGDRLLIELANRLESITTSGHLAARL
                     GGDEFAVLMPEATARDAFQYAARIRDSFARPIVIDGITLQTNVSVGIALFPDHASDAD
                     GLVQRADSAMYQAKHDGTGVALHKPDDLNDGLRRLQIIGDLRSAIANGELLLHYQPKV
                     CFEAGGIEGLEALVRWQHPTLGMLPPDEFIGLAEVSGLIQDLTMYVIRAAVADTARLR
                     EIGVDLPVAVNLSARNLYHPRLLAIILLALEREGVPTSAFRIEITESELMDDPSTAMV
                     VLQQLRDHGVQVSIDDFGTGYSSLSYLRDLPIDEIKIDRSFVADITDGDDVVVRSIID
                     LGHALGVDVLAEGVETVQQWHHLRRLGCDVGQGYLISRPLNFDALVRFLADRAEFGQP
                     PWVIERSQEPDTTSSQR"
BASE COUNT          564 a          999 c         1168 g          574 t
ORIGIN      
        1 ggcgccgggc agacgcgctg atcgtggtcc aaaagcgtcg cgatcgggcg tcggctctgg
       61 tggtgtcgat cccgcgggat ctcgtggtct ttcgcgccgg ggtgggcgcc gaccgactgg
      121 cgctgaccct gctcgacggc cccgccagca ccctcaacag cctctgtcgg acgttgggaa
      181 tcgagatcga ccatgctgcg gtcgtcgatt tcgccggtgt ggccgacatc gtcgatgggc
      241 ttggcggagt ggaggtgagc gccgagcacc cctcgcggga tcgggccacc gggctttcgt
      301 tggaggaggg caccaccctg ctcgatggga tcgaggctgt ggcgtgggcc cgttcgcggc
      361 acatggagca cctcgtcgat ggtcgctggg tcagcgtgga gggctccgac gacaaccgcc
      421 aggagaacca acgtgtgctg ctggagtcgg tcgtcgcgcg ccttcgctca cggtggtgga
      481 acctgctgca actggagcgg gttgcgtgga gcggatccca ggcggtgacg ctcagcgcct
      541 cgacggagct gatcgatgtc gcgcggctcg gtgcctcgtt ggggtccggc tacgacacga
      601 tcgaactgca tcacagcctc gacgatggcg acattcccgt cgctcgcctt caaggcgatg
      661 ccaacgcggt gttgaacgag ctcaacgctg gctcgacgtc gtgtccggtt cctgacttcg
      721 ttcaatgacc cacggcggct ggccgaactc ggcgcgatct gcgaggaacc gaacgagcgc
      781 atcgaaattc aacggcctgc tgatgaggta cccctgaccg acatcgcatc cgaggcgccg
      841 aaggtggtgc cactgttgga cggtctcgac accttcggcc aacacgtcga cgccgagtgc
      901 gtggcccagg tcgatgatcg aacgcacgac gacatcgtcg ccgtcggtga tgtcggcgac
      961 gaagctgcga tcgatcttga tctcgtcgat cggcaggtcg cgaaggtacg acagcgatga
     1021 gtagccggta ccgaagtcgt cgatcgacac ctgcacgccg tggtcgcgaa gttgttggag
     1081 gaccaccatc gcggtggacg ggtcatccat gagttcggat tcggtgatct cgatacggaa
     1141 ggccgaggtg ggaacgcctt cgcgttcgag cgccagcaag atgatggcga gcaggcgcgg
     1201 gtgatacagg ttgcgcgccg acaggttcac cgcgaccggc aggtcgacgc cgatctcgcg
     1261 caatcgtgca gtgtccgcta cagccgctct gatgacgtac atcgtgaggt cttgaatcag
     1321 ccccgaaacc tcggcgagac cgatgaactc gtcggggggc agcattccga gggtgggatg
     1381 ctgccagcgg accaaggctt cgagtccttc gatgcccccg gcctcgaagc agacctttgg
     1441 ctggtagtgc aacagcaatt cgccgttcgc aatcgcggag cggaggtcgc cgatgatctg
     1501 cagtctgcgg agaccgtcgt tgaggtcgtc gggcttgtgg agtgccacgc cggtgccgtc
     1561 gtgtttcgcc tggtacatcg cggagtctgc ccgttggacg agcccgtcgg cgtcgctggc
     1621 gtggtcggga aacagagcga tgccgacgct cacattcgtt tggagggtga tgccgtcgat
     1681 gacgatcggt cgggcgaagc tgtcgcggat tctcgctgcg tattggaatg cgtcgcgggc
     1741 cgtcgcctcg ggcatcagca ccgcgaactc gtcgccaccg agccgagccg cgagatggcc
     1801 gctggtcgtg atggattcca ggcggttggc gagttcgatc aggaggcggt cgccgtggtg
     1861 gtgccccagc gcgtcgttga cctccttgaa ctgatcaagg tccatcagga gcacggcgcc
     1921 gctcacatcg ttgacggttg tgaggacctc gctgatggcg ttgccgaaca tcgtccgatt
     1981 ggccagtccg gtcagcgagt cgtgaagcgc ctggacacgg agtgcgtcgg ccgcttgaac
     2041 ccggtcggtg acgtcgtcgc acacgacccc gatgtggtgt cgggcgatcg gaaacgcctg
     2101 cacggtgatc caccgctcgg tcccggcacc gtcgctccac cgcacctcgg agggctgcat
     2161 gggggtctgg gtgcgatcga cctcggcgaa ggccgcgagg tgttgtggcc aggtgtcgac
     2221 gaaccgactg gcttctggcc agccctcggt ggattcggtg accggaagtc cgatcgcctc
     2281 ctccgcggcc ttgctggcag agatcagttc gaactccggc acctcgccgc tatggtcgac
     2341 cttcacgatt tccaacgcgt aggggatctg ggtcaggaaa tcgccgagcg ccgccatctc
     2401 ccgctcctgg gcatggttcc tcgtgacgtc gatgaagatg ccccacaggc cctcttcacg
     2461 ccagacgacc tgggcgagca gatagacggt gcgttcgtcc tgtccgcgta cccgaagcgt
     2521 ggtgaggtgc gaggtgccgg gagtgccggt ttcgagggtg gacaaaaacg cctcgacatc
     2581 ggccggatgc agccgctcaa gccacgttgt ccgacgggtc cattcgtcgg tggagtaacc
     2641 cagcacctgt tcgatccggt cgctgaaaaa cctgaagttg ggggagcctg caggcgaatc
     2701 gaacgcgatg atgtcgagcc ggtcgagcag gtcggtgaat cgggttcgga cccgcaacgc
     2761 ttcgtcggca gactgggcga tcaacgtcgt cgtgctgaaa cccgccgtgg cgaacagcgc
     2821 tactgcgggt gcccagttcg gaaggccagc acccaagccc accaaggcgt agaaggcggt
     2881 cccaccgagg agcgaccagg tggcgatccg acgcccatgg tgaatgtgct gaccgggtat
     2941 ggacacgacg gtgatacaca cgccccactg aaacagttgg gggacgacga ccccgacggc
     3001 cgccaccgcg gcgaagtcga ggaaggtcaa cagcgcgagt gatcgcgggt gacgcgcggc
     3061 gaggtgccag aacaggatga tttcggcggc tccgatggcg acggccgcgg cggcggtcgc
     3121 caatcgatgt ggcccgaact gcgacacctg ggtgatgatc accgcgagcg gcaccgtgag
     3181 cgagcgttgg gtcaccaagt ggcgccgaag caggcgggtc gacgacgtgg agacgtgcgc
     3241 ggcgccaccg gtgcggcgcc cgtcgaaagc gggggcgcgc tgggggccgg gcggtcgaag
     3301 cagta
//
LOCUS       DAOOMA010000273         3301 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_35137_length_3301_cov_3.862600, whole genome shotgun sequence.
ACCESSION   DAOOMA010000273 DAOOMA010000000
VERSION     DAOOMA010000273.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 3301)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3301)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3301
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35137_length_3301_cov_3.862600"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..239
                     /locus_tag="PLP95_08060"
     CDS             <1..239
                     /locus_tag="PLP95_08060"
                     /inference="COORDINATES: protein motif:HMM:NF015043.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016702 - oxidoreductase activity, acting
                     on single donors with incorporation of molecular oxygen,
                     incorporation of two atoms of oxygen [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="carotenoid oxygenase family protein"
                     /protein_id="HPB45791.1"
                     /translation="IIDMGPGRVAGEWVMVPRHESAAEDDGWLMSLVYDAGTDRSELV
                     VLSASDPTAGPVARVLLPNRVPHGFHGNWVPDGA"
     gene            complement(361..1317)
                     /locus_tag="PLP95_08065"
     CDS             complement(361..1317)
                     /locus_tag="PLP95_08065"
                     /inference="COORDINATES: protein motif:HMM:NF012976.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB45792.1"
                     /translation="MTCEPQPAATPTSESTAVERSAHHVELSRRTVLRGVFGGVVALP
                     AAAMLSSCSEDDGASESQSTTTAGGAGSTTATGSSTTAPPQTAPTPAWASGGTDLITV
                     DYPSTSIFATATACTVTLTSALMEGPCYFQSDTSDDISVGTTGLPMQLCLQLIDESCA
                     PLDGYTVEVWHCDARGIYSGDTSESHDAARFAGAFCTEDDPQALESSYCRGQATTDAE
                     GRVNFKTIFPGWYSGRTIHIHFAVSDADGTSRIISQWCYVDELAEEIYTTHDRYSGRG
                     SQDTTLASGTDGVFPNDYEPFVLNTRANADGSMLSYGVIQIA"
     gene            complement(1399..2697)
                     /locus_tag="PLP95_08070"
     CDS             complement(1399..2697)
                     /locus_tag="PLP95_08070"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HPB45793.1"
                     /translation="MKRRILAAIVGVAVVCLGLLSLPLGWYIISNERDGVDRNFSLIA
                     ERAVNALTLDGDFGANGNYPLPDYREPVDVAVYDVEGRLVAGVGPKWADRITDSAHLL
                     TLTGTTRTERVLARPVVAHQEKIGTVRVAEPLSAAGRRVAKVLVLLGGMNLVILGGAV
                     VVGRWLSGRLVQPLSDIRDDAVRLGQGDFALAPLRSGIAELDATSEALADTAARLDAV
                     LQRERSFTADVSHQLRTPLTSLRLTLENELDHPSEPPDEAIAGALADADRLESTITTL
                     LRVARDQPTSRDALDLDSWAGSIRDRWNETFDAAGRSIALTAEDGRAHVSGAVLDQIV
                     DILIQNALDHGSGRVSVTVERRDDHLDVVVGDDGSLARATEDLFERRDPGAHGHGLGL
                     ALGRSLAEAEGGRLVVASSSPTSFRLVLPAGRFAQPRTAR"
     gene            complement(2694..>3301)
                     /locus_tag="PLP95_08075"
     CDS             complement(2694..>3301)
                     /locus_tag="PLP95_08075"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HPB45794.1"
                     /translation="CIAAPDAATARAIANAPNLILLDLGLPDADGLDLARELGGRWPS
                     VPIMILTARAEEMDIVIGLDVGAVDYVVKPFRLAELMARIRAHLRQVALVDDDTDQHL
                     SSAGIDVDLGARTVRVDGAEVALRVKEFDLLVELIAHEGRVVTREDLMANVWDEHWFG
                     STKTLDVHVASLRRRLGEAPGSPSRITSIRGVGYRWEREPR"
BASE COUNT          536 a         1050 c         1141 g          574 t
ORIGIN      
        1 agatcatcga tatggggccg ggccgtgtgg ccggcgagtg ggtgatggtg ccgcgccacg
       61 agagcgcggc cgaagacgac ggctggttga tgagcctggt ctatgacgct gggacggatc
      121 gcagcgaatt ggtggtgctg tcggcctcgg atccgacggc gggtcccgtc gctcgcgtgc
      181 tgctgcccaa ccgggtgccg cacggctttc acggcaactg ggtgcccgac ggcgcgtgag
      241 cgcggcggcg ccggtcgggc agtgatcgag tcgggtgttg tacatgaggg gcgcggtatc
      301 gcgcccctca tgtacaacac ccgggccgca gcactcggcc gcgcccccgg ccggcgcccg
      361 tcaggcgatc tggatgacgc cgtaactgag catcgaacca tcagcgttcg cccgggtgtt
      421 gagcacgaag ggttcgtagt cgttcgggaa cacgccgtcg gtgccactcg cgagggtcgt
      481 gtcttggctt ccccggccgg agtatcgatc gtgggtcgtg tagatctcct cggcgagttc
      541 atcgacgtag caccattgcg agatgatgcg gctcgtgccg tcggcgtcgc tgaccgcgaa
      601 gtgaatgtgg atggtccgcc cggagtacca gcccggaaag atcgtcttga agttcaccct
      661 gccctcggca tcggtcgtcg cttgaccgcg gcagtagctg ctctcgagcg cctgtgggtc
      721 gtcctcggtg cagaacgctc cggcgaatcg tgcggcgtcg tggctttcgc tggtgtcgcc
      781 cgaatagatg ccgcgtgcgt cgcagtgcca cacctcgacc gtgtatccgt cgagtggagc
      841 gcagctctcg tcgatgagtt gcaggcacaa ctgcatgggc aacccggtgg tgccgacgga
      901 gatgtcatcg ctggtgtcgg attggaagta gcacggtcct tccatcaatg cggaagtgag
      961 tgtcacggtg cacgccgtgg cggtggcgaa gatcgacgtc gacggatagt ccacggtgat
     1021 gaggtcggtt cccccagacg cccacgctgg cgtcggtgcg gtttgcgggg gtgccgtggt
     1081 cgaggaccca gtcgctgtgg tcgagccagc accgccagcc gttgtggtgc tttgcgactc
     1141 cgaggcgccg tcgtcctcgc tgcacgaact caacattgcg gcggcgggta gggcgaccac
     1201 acctccgaac accccgcgga gcaccgtgcg ccgagacagt tccacgtggt gcgcggatcg
     1261 ctcgaccgca gttgattcgg acgtcggtgt cgcggcgggt tgtggctcgc aggtcatggc
     1321 ggcagtcttg caccgcggtc gcgtcctgga gggggcgagt cctggagggg tcgatcgccg
     1381 gcgttggccc ccgccgcgtt agcgggcggt gcgaggttgg gcgaagcgcc ccgccggtag
     1441 gaccagtcga aacgaggtcg gactgctcga tgcgaccacc aagcggcctc cttcggcctc
     1501 ggcgagcgag cgtccgagtg caagccccaa gccgtgaccg tgggctccgg ggtcgcgtcg
     1561 ttcgaaaagg tcctcggtgg cccgtgcgag tgatccgtca tcgcccacga ccacgtcgag
     1621 gtggtcatcg cgcctttcga cggtcaccga aacgcgtccg gatccatgat ccagcgcatt
     1681 ctgaatcagg atgtcgacga tctgatcgag gaccgcaccg ctcacatgcg ctcgaccatc
     1741 ctctgcggtg agcgcaatgg agcgcccggc cgcgtcgaag gtttcgttcc aacgatcgcg
     1801 gatggaaccc gcccaactgt cgaggtcgag ggcatctcgt gacgtcggtt gatcgcgggc
     1861 gactcgaagc agtgtggtga tcgtggattc caaccggtcg gcgtcggcga gggcgccggc
     1921 tattgcctcg tcgggtggct cgctcgggtg gtcgagttcg ttctcgaggg tcaagcgaag
     1981 cgaggtcagc ggtgtgcgaa gttggtggga aacatcggcg gtgaacgagc gttcgcgctg
     2041 aagcaccgca tcgagacgtg ccgcggtgtc ggcgagtgct tcagaggtag cgtcgagttc
     2101 ggcgattccc gatcgaagcg gtgcaagagc gaaatcccct tgccccagcc gcaccgcatc
     2161 gtcgcggatg tcgctcaacg gctgcaccaa ccggccgctc aaccatcgcc cgaccaccac
     2221 cgcgcccccg aggatcacaa ggttcatgcc gccgagcagc accagcacct tcgccacccg
     2281 acgacccgcg gccgaaaggg gttcagcgac gcgaaccgtg ccgatcttct cctgatgcgc
     2341 aaccacgggg cgggccagca cccgttcggt gcgcgtggtt cccgtgagcg tgagcagatg
     2401 agccgaatcg gtgattcgat ccgcccactt cgggccgact ccggccacga gtcgaccctc
     2461 aacgtcgtac accgccacat cgaccggctc gcggtaatcg ggcagcgggt agttgccgtt
     2521 ggcgccgaag tcgccatcga gggtgagcgc gttcaccgcc cgctcggcga tcagcgagaa
     2581 gttgcggtcg accccgtcgc gctcgttgga gatgatgtac cagccgagtg ggaggctgag
     2641 caggccaaga cacaccaccg caaccccgac gatcgcggcc aagatccggc gcttcatcgc
     2701 ggttcgcgct cccagcgata gccgacacct cgaatcgagg tgatccgcga gggcgatccc
     2761 ggggcctctc cgagtcgtcg ccgcaatgag gcaacgtgaa cgtcgagggt cttggtcgac
     2821 ccgaaccaat gctcgtccca cacgttcgcc atcagatctt cgcgggtgac cacgcgcccc
     2881 tcatgggcga tgagctccac cagcaggtcg aactctttga cgcgcaacgc cacctcggca
     2941 ccgtccacgc gcactgtgcg tgcacccaga tcgacatcga ttccggcgct cgacaggtgt
     3001 tggtcggtgt cgtcgtcaac cagggcgacc tggcgcaggt gggccctgat tcgggccatc
     3061 aactcggcga gtcgaaaggg tttcaccacg tagtcgaccg cgccgacgtc gaggccgatc
     3121 acgatgtcca tttcctcggc ccgcgcggtg aggatcatga tcggaacact cggccatcga
     3181 ccaccgagtt cgcgtgccag gtcgagtccg tcggcgtcgg gaagcccgag atccaacagg
     3241 atcaggttcg gtgcgttggc gatggcacgg gcggtggccg cgtcgggggc ggcgatgcac
     3301 t
//
LOCUS       DAOOMA010000274         3275 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_35525_length_3275_cov_3.266770, whole genome shotgun sequence.
ACCESSION   DAOOMA010000274 DAOOMA010000000
VERSION     DAOOMA010000274.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 3275)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3275)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3275
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35525_length_3275_cov_3.266770"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..866)
                     /locus_tag="PLP95_08080"
     CDS             complement(<1..866)
                     /locus_tag="PLP95_08080"
                     /inference="COORDINATES: protein motif:HMM:NF033484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HPB45795.1"
                     /translation="MRLNVGAATDVGQLRTLNEDSYAVAADLVAVADGMGGHRAGEVA
                     SALALSTFQLAFSSRDEAAMVAAVDAANAAVFEHASSDLQLSGMGTTLCAAAVIGDGE
                     IAAVNVGDSRIYLLRDGELRQISEDHSFVEALVREGRLTRAQAEVHPQKNVITRALGV
                     EADVLVDAWTVPVQDGDRIVLCSDGLTNEVPEQTIEDALRQFNDPVEVCGRLITLANI
                     GGGRDNITVVVADIAESTTSPEVLEPIGRPIERFEVAELPSGGVVETADQATVIAPTG
                     LAPSAAPVSEPS"
     gene            complement(866..1417)
                     /locus_tag="PLP95_08085"
     CDS             complement(866..1417)
                     /locus_tag="PLP95_08085"
                     /inference="COORDINATES: protein motif:HMM:NF028007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HPB45796.1"
                     /translation="MPDQLLTIFRLCFLVLLYLFFFRVLRAIWTEVNSVPAPFDGPAP
                     KLSRKARKAIRSAPGDGAGAPPAPITTSPPPEPTVSPTTSRRLGTPNALRVIDPPHLA
                     GVDYPLADNLIFGRGGSAAVMFDDAFMSTLHLRITATGRRWFAEDLGSTNGTYINRVR
                     VTGIVELSLGDQIQMGNVVLEVV"
     gene            complement(1417..2076)
                     /locus_tag="PLP95_08090"
     CDS             complement(1417..2076)
                     /locus_tag="PLP95_08090"
                     /inference="COORDINATES: protein motif:HMM:NF023818.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3662 and FHA domain-containing protein"
                     /protein_id="HPB45797.1"
                     /translation="MGLSNFERKLERGVEGVFGRVFRSEVRPVEIGRKLVREMDVART
                     VGVNGQTLVPNSYLVVVSPSDHEQFADMVDPLSGELMALLKQHAKDERYRFVGPLAVS
                     FEVDEQQRPGVVGIDSRFRQPNAGDVMAHLLLPGGQRVPLSVEAVSVGRSPDCTVQLG
                     DQNASRRHAEIRPVEGGYAVVDLASTNGTLVNGRRVAEHILNDGDDITFGQTTIRYER
                     A"
     gene            2183..2269
                     /locus_tag="PLP95_08095"
     tRNA            2183..2269
                     /locus_tag="PLP95_08095"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2217..2219,aa:Leu,seq:cag)
     gene            complement(2411..3097)
                     /locus_tag="PLP95_08100"
     CDS             complement(2411..3097)
                     /locus_tag="PLP95_08100"
                     /inference="COORDINATES: protein motif:HMM:NF020387.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RES family NAD+ phosphorylase"
                     /protein_id="HPB45798.1"
                     /translation="MTLRDPYRDISKFPVMVLAEGTALWRVTKREFAAEPWFFATDAN
                     GRFNLVLPRPEGTCYFADDAEPCVAETLWRDREPTGDPDIVPDITAEEAANYCAVRVT
                     LERDYRCADLTFAKGTRISQAFGFNSEINTSVSYLYPQQWADAFAEEGLDGVAYVASR
                     TSGASSYALFGPAGQTSTAATEVHELSPRFLNDHNMAPARRHPGSGHRLIADNAEALA
                     ERAQGRVRRR"
     gene            complement(3094..>3275)
                     /locus_tag="PLP95_08105"
     CDS             complement(3094..>3275)
                     /locus_tag="PLP95_08105"
                     /inference="COORDINATES: protein motif:HMM:NF021255.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2384 domain-containing protein"
                     /protein_id="HPB45799.1"
                     /translation="VEHLAPSLVEANSHSGAVRWLATPNRQLEGRAPWDAIDDGDSDA
                     VIAAAQRQIAAWSGR"
BASE COUNT          575 a         1052 c         1046 g          602 t
ORIGIN      
        1 tcggagggct ccgacaccgg tgccgcggag ggtgccagac cggtcggggc gatcacggtg
       61 gcctgatcag cggtttccac caccccgcca ctcggcagtt ctgcaacctc gaatcgctcg
      121 atcgggcggc cgatcggttc gaggacctcg ggggaggtcg tcgactcggc gatgtcggcg
      181 accacgacgg tgatgttgtc gcgaccgccg ccgatgttgg ccagggtgat caggcgacca
      241 cagacctcaa ccgggtcgtt gaattggcgg agcgcgtcct cgatcgtctg ctcgggaacc
      301 tcgttggtca gcccgtcgga gcacaacacg atgcgatcgc cgtcttgcac ggggaccgtc
      361 cacgcgtcga ccaacacgtc cgcttcgacc ccgagcgcac gggtgatgac gtttttttgg
      421 ggatgcactt cggcctgggc ccgcgtcagg cggccttcgc gaacgagggc ctcgacgaag
      481 ctgtggtctt cggagatctg cctcagttcg ccatcacgca agagatagat ccgagaatcg
      541 ccaacgttga cggcagcgat ctctccatcg ccgatgaccg ccgccgcgca caacgtggtt
      601 cccattccgg acagttgcag gtcgctcgac gcgtgttcga acaccgcggc gtttgccgca
      661 tcgaccgccg cgaccatcgc tgcttcgtcg cggctggaaa aggcgagctg aaaggtcgac
      721 agcgcaagcg cggatgcaac ctctccggcg cggtgcccgc ccatgccatc ggcgacggcg
      781 acgaggtcgg cagcgaccgc gtagctgtcc tcgttcagcg ttcgaagctg gccgacatcg
      841 gtggccgcgc ccacgttcag acgcatcaga cgacctccaa cacgacattg cccatctgga
      901 tctggtcacc gagggaaagc tcgacgatgc cggtgactcg aacccgattg atgtacgtgc
      961 cgttggtcga cccaagatcc tccgcaaacc atcgacgacc ggttgcggtg atgcgcaggt
     1021 gcaacgtcga catgaacgcg tcgtcgaaca tcacggccgc gctccccccg cgtccgaaga
     1081 tcaggttgtc ggcgagcggg tagtcgacgc ccgccaagtg aggtgggtcg atcacccgca
     1141 aggcattcgg ggtgccgaga cgtcgagacg tcgtcggcga aacagtcggc tccggaggcg
     1201 gactcgtcgt gatcggcgcc ggcggtgcac cagccccgtc accgggtgcc gaccgaatcg
     1261 ccttcctcgc ctttcgcgac agcttgggcg caggaccgtc gaatggagcc ggcaccgaat
     1321 tcacctcggt ccagatcgct cgtagcaccc gaaagaaaaa caggtagagc agcacgagga
     1381 aacagagccg gaagatcgtg agcaactgat cgggcatcag gccctctcgt atcggatcgt
     1441 ggtctggccg aaggtgatgt cgtcgccgtc gttcaagatg tgctcagcga cgcgacgacc
     1501 gttgacgagt gtgccgttcg tcgacgccag gtcgaccacc gcgtagccgc cctccaccgg
     1561 acggatttcc gcgtgacgcc tcgacgcgtt ctggtcgccg agttgcaccg tgcagtccgg
     1621 agaacgtccc accgaaaccg cttcgacgct gagtggcacc cgttgccccc caggcaacag
     1681 cagatgggcc atgacgtcac cggcattcgg ctgacgaaac ctggagtcga tgccgaccac
     1741 tccgggacgt tgttgctcgt ccacctcgaa gctcaccgcg agcggaccca cgaatcggta
     1801 tcgctcgtcc ttcgcgtgct gcttgagcag tgccatcaac tccccggaca gcgggtcgac
     1861 catgtcggcg aactgctcgt gatccgatgg actcaccacg accaggtagc tgttcggtac
     1921 caatgtctga ccgttgacgc ccaccgtacg ggcgacgtcc atctcccgga cgagcttgcg
     1981 gccgatctcg acggggcgaa cctcactgcg aaagacgcgg ccgaagacgc cttccacacc
     2041 gcgctcgagc tttcgttcga agttcgacaa ccccacggcg cgaaggctac ttggccacca
     2101 tgtcgcgatc gggccacgac gggaggccga tctcagctac cacttcctcg tgagcgcggc
     2161 tattgttgcg acgcttactc ttgcgggagt ggcggaattg gcagacgcgc aggattcagg
     2221 ttcctgtgtc cgaaagggcg tgggggttca agtcccccct cccgcaccag atgttcgtta
     2281 catgaactca gactgttgag agtcgccgaa gccacgagag tgggtcggcg actcttgcat
     2341 ttcaagccac ctcggtggcc ggtgacgcgc tcactgaact gctcagcgcc gagcttctga
     2401 gcctcgctgg tcaacgcctg cggactcgtc cctgggcacg ttcagcgagt gcctctgcgt
     2461 tgtcagcgat cagtcgatgg cctgagcccg ggtgccgcct tgctggtgcc atgttgtggt
     2521 cgttgaggaa cctcggtgac agctcatgga cttcggtcgc tgcggtcgat gtctgtcccg
     2581 cgggaccgaa cagcgcgtag ctggatgcgc cagaggttcg tgacgcgacg taggccacac
     2641 catcgagtcc ttcttcagcg aacgcgtcgg cccactgttg ggggtacaga tagctcactg
     2701 aggtgttgat ctcgctgttg aacccgaagg cctgtgagat ccgggtgccc ttcgcgaacg
     2761 tgaggtccgc acatcggtag tcacgctcga gggtgactcg gacagcgcag tagtttgcgg
     2821 cttcctcggc tgtgatgtcg ggaacgatgt cggggtcgcc cgttggttct ctgtcacgcc
     2881 acagcgtttc tgcgacgcag ggttcggcgt cgtcggcgaa gtagcaggtt ccttcgggtc
     2941 gaggaaggac gaggttgaac cggccgttgg catcagtggc gaagaaccac ggttccgctg
     3001 caaattctcg tttggtgacg cgccacagcg cggtgccttc ggccagcacc atcactggaa
     3061 acttcgagat gtcgcggtac gggtcccgta acgtcaccgg ccgctccacg cagcgatctg
     3121 acgctgtgcg gccgcgatga ccgcgtcgct gtcgccgtca tcgatcgcat cccacggggc
     3181 tcggccctcg agttgtcggt taggcgtcgc cagccagcgc acagcgccac tgtggctgtt
     3241 tgcttccaca agggagggtg caagatgctc caccg
//
LOCUS       DAOOMA010000275         3273 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_35561_length_3273_cov_3.353947, whole genome shotgun sequence.
ACCESSION   DAOOMA010000275 DAOOMA010000000
VERSION     DAOOMA010000275.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 3273)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3273)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3273
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35561_length_3273_cov_3.353947"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1169)
                     /locus_tag="PLP95_08110"
     CDS             complement(<1..1169)
                     /locus_tag="PLP95_08110"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HPB45800.1"
                     /translation="MTRPAALNIWALAGEAAAKTPPSRDRVVDLLRGGSLAVVVIGHT
                     LMAVVTWKGSTPVIGNLLAEIPELQVLTWVLQVMPVFFAAGAIANRASWNAAGAKARQ
                     GSTQEGSKQDWAAWMWGRTARLMRPVVWFLAVWIPLVIVLRVAVGPDADPLARLSTQL
                     LWFLGVYIVVVAFTPSEVRWARRGPLPVVAMLGAVAVVDLVRFNTHDAVGLINFVLVW
                     VMAATLGLVARDRIDRPRLLLGWAAAALAVEIAVVALGPYPMSMVGMPGEKVSNMAPP
                     TLALALHSVVLICVVSAGWNPLHRWCRRPRVWHTVCAVGAVAMSLYLWHLSALIVVTV
                     LQNALNLNRPEVGAAGFWPATLVHTVVCLVVVVGVVLAAAPLEHLRVPWLETPARR"
     gene            complement(1211..1744)
                     /locus_tag="PLP95_08115"
     CDS             complement(1211..1744)
                     /locus_tag="PLP95_08115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB45801.1"
                     /translation="MGVDLRHRVPRFFESLKPGPPPPEDVDWALSLLNEREQTLFHRM
                     SNPDQRHAIRVARRVETSYPPVAGETGVPVATALVAALLHDVGKIEAGLGTYGRVAAA
                     GCEVVARDMAEIWQQRSGMTRRIGLYLRYTEIGVDLLRLAGSEEVVVAWSREHHLDEE
                     QWSVPIAFGRVLAAADE"
     gene            complement(1744..2661)
                     /locus_tag="PLP95_08120"
     CDS             complement(1744..2661)
                     /locus_tag="PLP95_08120"
                     /inference="COORDINATES: protein motif:HMM:NF001399.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009037 - tyrosine-based site-specific
                     recombinase activity [Evidence IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA];
                     GO_process: GO:0007049 - cell cycle [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HPB45802.1"
                     /translation="MGAGDEGLPAAAESFLLWLTVERGRSANTLAAYRRDLGGYVDWL
                     GSRGLNVSTVGHDDVLSWVVELRDGLAASSVARKVSVVRALHRWMASEGLSEVDPTLR
                     LESPRQRDPIPKALSETDIERLLGAATASENLRDIALLEVLYGSGLRISEAVGLNLTD
                     VDLEAKLMRVFGKGAKERIVPIGRVAQHALARYLDDGRAAMVPRRPRSRDDADAVFLN
                     QRGGRLTRQGGWLILDRHARSVGLSVSPHVLRHSCATHMLDHGADIRAVQELLGHASI
                     STTQRYTKVMTDRMWRVYEQAHPRAGSVS"
     gene            complement(2672..>3273)
                     /locus_tag="PLP95_08125"
     CDS             complement(2672..>3273)
                     /locus_tag="PLP95_08125"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HPB45803.1"
                     /translation="AQPDDPGGVVTVADASAGDEVQFHKVSEELIHQGFVIGVYNVRF
                     EGPGGVQMDRDVVRHPGAVSVVPVLEDNTVVLVRQFRSALEHNILEIPAGKRDVDGEA
                     PEVTARRELSEEVGYEAGSLVKLLELTHSPGFCDEVNHIYLATDLTPTERSVDGPEEE
                     AMTIEHVALADVFDLISSGAITDAKSVAGLTMAYHRLLR"
BASE COUNT          571 a         1202 c         1039 g          461 t
ORIGIN      
        1 cgccgcgctg gcgtttccaa ccagggaacc ctgaggtgtt cgagcggcgc cgcggcgagc
       61 accaccccga ccacgaccac caagcacacc accgtgtgca cgagcgtcgc cggccagaac
      121 ccggcggccc cgacctccgg gcggttcagg ttcagggcgt tctgcagcac cgtgacaacg
      181 atcaacgcgc tgaggtgcca cagatacaac gacatggcga ccgcccccac cgcgcacacc
      241 gtgtgccaca cccgaggtcg tcgacaccag cgatgcagcg ggttccaccc cgctgaaacc
      301 acacagatca gcaccaccga gtggagggcg agggcgaggg tgggcggcgc catgttggac
      361 accttctccc ccggcatgcc gaccatcgac atcgggtacg gccccaacgc cacgaccgcg
      421 atctcgacgg cgagcgcggc cgccgcccag ccgagcaaca gtcgtggccg atcgatccgg
      481 tcccgcgcca cgagtcccaa cgtggcggcc atcacccaga cgagcacgaa gttgatgagc
      541 ccaaccgcgt cgtgggtgtt gaaccgcacc agatccacca ccgccaccgc gcccaacatc
      601 gccacgacgg gcagcggccc acgtcgagcc caacgcacct cgctcggggt gaaggccacg
      661 accacgatgt agacgccgag aaaccacagg agttgggtcg acagccgcgc gagcgggtcg
      721 gcatcggggc cgacggcgac cctcagcacg atcaccagcg gaatccacac cgcgaggaac
      781 cacaccacgg ggcgcatgag ccgagcggtg cggccccaca tccacgccgc ccagtcctgt
      841 ttcgagccct cctgcgtcga accctgccgg gccttcgcac ctgcggcgtt ccacgatgca
      901 cggttggcga tcgcaccggc ggcgaagaag accggcatga cctgcagaac ccaggtgagc
      961 acctgcagct cggggatctc cgccaacagg ttgccgatca ccggcgtcga tcccttccac
     1021 gtgaccaccg ccatcagcgt gtgcccgatc acgaccacgg cgaggctgcc cccacgcaac
     1081 agatcgacga cccgatcgcg actcggcggc gtcttggccg ccgcctcgcc ggccaacgcc
     1141 cagatgttga gagctgctgg ccgagtcatc ggtgcgacgt taggcggcgc cgcgggccgg
     1201 agcggcaccc tcactcgtcc gctgcggcga gtacccggcc gaaggcgatc ggcacgctcc
     1261 actgctcctc gtcgaggtga tgttcccgcg accaggccac gaccacctcc tcggaaccgg
     1321 cgaggcgcaa caggtcgacc ccgatctcgg tgtagcgaag gtacagcccg atgcgtcgcg
     1381 tcatgcccga acgctgctgc cagatctcgg ccatgtctcg ggccacgacc tcacagcccg
     1441 cagcggccac acggccatag gtgccgagcc ccgcctcgat cttgccgacg tcgtgcaaca
     1501 gcgcagcgac cagcgccgtc gccaccggca ccccggtctc gccggccacc ggcggatacg
     1561 acgtctcgac ccggcgggcg acacgaatcg cgtgacgctg atcggggttc gacatccgat
     1621 gaaacagcgt ctgttcgcgc tcgttgagca gcgacagcgc ccagtcgaca tcctcaggcg
     1681 gtggaggccc cggcttgagc gattcgaaga accgcggcac gcggtggcga aggtcgactc
     1741 ccatcaactc accgatccgg cccgggggtg ggcctgttca tagacgcgcc acatccggtc
     1801 ggtcatcacc ttggtgtagc gctgggtggt cgagatcgac gcatggccga gcagttcctg
     1861 caccgctctg atatcggcgc cgtggtcgag catgtgagtg gcacacgagt ggcgcaggac
     1921 gtgtggcgac accgacagcc caaccgatcg ggcgtgcctg tcgaggatca gccacccgcc
     1981 ctggcgggtc agtcggccgc cccgctggtt gagaaacacg gcatccgcgt cgtcacgcga
     2041 gcgcggtcgt cgcggcacca tcgccgcccg accgtcgtcg aggtaccgtg ccagggcgtg
     2101 ctgggccacc cggccgatgg gcacgatgcg ttccttcgcc cctttgccga acacccgcat
     2161 gagcttggcc tcgaggtcga cgtcggtgag gttcagtccc accgcctcgg agatccgcag
     2221 ccccgacccg tacagcactt ccagcaacgc gatgtctcgc aggttctccg atgcggtggc
     2281 cgctcccagc agacgctcga tgtcggtttc gctcagcgcc ttggggatgg ggtctcgttg
     2341 gcggggcgac tcgaggcgca acgtggggtc gacctccgag agtccctccg aggccatcca
     2401 gcggtgaagg gcacgaacca ccgacacctt gcgtgccacc gaactcgccg ccaacccatc
     2461 gcgcagttcc acgacccagc tcaacacgtc gtcgtggccg acggtggaca cgttcaggcc
     2521 gcgggacccc agccagtcga catacccgcc gaggtctcga cgatatgcgg cgagggtgtt
     2581 ggccgaacgt ccgcgttcca ccgtgagcca cagcagaaac gactccgccg ctgcgggcag
     2641 gccctcgtcg ccggcgccca tcggccgacg ctcagcgcag gaggcggtgg taggccatcg
     2701 tcaggcccgc caccgacttg gcgtcggtga tcgcaccgct cgagatgaga tcgaagacgt
     2761 ccgcgagcgc gacgtgttcg atcgtcatcg cctcctcctc ggggccgtcc acgctgcgtt
     2821 cggtcggggt caggtcggtg gccaggtaga tgtggttcac ctcgtcgcag aacccggggg
     2881 aatgggtgag ttcgagcaac ttgacgagcg aacccgcctc gtagccgacc tcctcgctca
     2941 gctctcgccg ggcggtcacc tcgggggcct cgccgtcgac gtcgcgtttg ccggcgggga
     3001 tctccaagat gttgtgttcc agcgccgagc ggaactgccg aaccagcacg acggtgttgt
     3061 cctccaacac gggaaccacc gacaccgccc cggggtgacg aaccacgtcg cggtccatct
     3121 gcaccccgcc cgggccctca aaacgcacgt tgtagacgcc gatcacgaac ccctgatgga
     3181 tgagctcctc ggagaccttg tggaactgca cctcgtcgcc ggcgctggcg tcggccaccg
     3241 tcacgacacc tccaggatcg tcgggttgcg ccc
//
LOCUS       DAOOMA010000276         3268 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_35654_length_3268_cov_3.521942, whole genome shotgun sequence.
ACCESSION   DAOOMA010000276 DAOOMA010000000
VERSION     DAOOMA010000276.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 3268)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3268)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3268
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35654_length_3268_cov_3.521942"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(141..902)
                     /locus_tag="PLP95_08130"
     CDS             complement(141..902)
                     /locus_tag="PLP95_08130"
                     /inference="COORDINATES: protein motif:HMM:NF014708.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HPB45804.1"
                     /translation="MTTTTSTRADIRRARQRFHTDIGWLDSWHSFSFGHHFNPNELGH
                     GLLIVSNDDVVAPGGGFGSHPHADMEIVTWVLDGELEHRDSEGNHGVIGPGLAQRMSA
                     GTGIVHSEFNASSTAPARLVQMWVVPSQTGITPGYEQVDVTDRLDAGGWVAIASGQGH
                     EGAVTIHQPGAVMWVARMGERERLDVPRAPYVHVFVATGTVAVDLEDLAGDQWNGTTL
                     NQGDALRLTGEQAAALTALERAEVIVWESDRQARR"
     gene            complement(1006..2520)
                     /locus_tag="PLP95_08135"
     CDS             complement(1006..2520)
                     /locus_tag="PLP95_08135"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF013592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HPB45805.1"
                     /translation="MSAPRTFARLSTVAGTVAAISLVAAVSSCSAGGSGEATSVVDDA
                     ASATTTTATTTSVATMNGSASLDDPIVAGSGNGGYDVTRYVVDFDATDANRGGTVAAT
                     VGIELSTTADAPLASFNLDFAGFEIDEVLVDGSAATVARDGGELVVTPPSPISGDHVV
                     TITYSGTPSTIDDVTSPGSIGWLRSPTGSFTAAEPNGMHGIIPCNDHPSDPAQFHITI
                     HAAAGETAVASGRSGEVITHPDGSTTSQFTVSDDIPLYALQMAVGDYEVTTHDVALSQ
                     STVTIRHAVPRSAPAAVTEQLGLTEAQLRWFDATVGPVPEGIYGVFVPDTPLAFALET
                     HTLSMIPLDWFTSGSTELVAEVTAHELAHQWFGNLVNLGSWSDLWLSEGWATYLSWMW
                     SAASGGASVQSQADAIRAGFDSSGVSMDAIADPDPHDLWGPLRYEGGALALHALRAEV
                     GEEAFSTIIAGWPAEFADASVTTSDFVAYASKIAGRDLGAFFDAWVYSTSLPSA"
     gene            2702..>3268
                     /locus_tag="PLP95_08140"
     CDS             2702..>3268
                     /locus_tag="PLP95_08140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012226402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HPB45806.1"
                     /translation="MSNFSLALNDDQLQLQEWVHGFAKDVIRPAGEEWDEKEEFPWPI
                     VQEAAKIGLYGMDFMANALFDPTGLSMVLAVEELFWGDAGIALSIMGSGLAAAAISAN
                     GTQEQTLEWVPQCYGTAEKVQLGALCASEPDAGSDVGGYRTRAVYDEKTDTWTLNGTK
                     AWITNGGIADIHVIVAVVEPGIGSRGHAS"
BASE COUNT          548 a         1027 c         1155 g          538 t
ORIGIN      
        1 cgcgcgctga tgagttacgc gacgaactgg tggggctggg ttacgtggtg gaggacaccg
       61 cccagggcac ccgggtgcgc acggcctgag aagtccgcct gtttgccggt tcctggccgc
      121 agcgacggat cagcggcgga tcagcggcgg gcctggcggt ccgactccca cacgatcact
      181 tcggcgcgtt cgagtgcggt gagcgccgca gcctgttcgc cggtcaggcg caacgcgtcg
      241 ccttggttca gcgtcgtgcc gttccactgg tcgccggcca ggtcctcgag atcgaccgcg
      301 acggtgccgg ttgccacgaa gacgtgcacg tagggggcgc gcggcacgtc gaggcgttcg
      361 cgttcgccca tcctcgcaac ccacatcacg gcgccgggct ggtggatggt gacggcgccc
      421 tcgtggcctt gaccgctggc gatcgcgacc cagccgccgg cgtcgagtcg atcggtgaca
      481 tcgacctgtt cgtagccggg ggtgatgccg gtttgcgacg ggacgaccca catctgcacc
      541 aaccgtgcgg gagcggtgct cgacgcgttg aactcggagt ggacgatgcc ggttccagcg
      601 ctcatgcgtt gggccagccc ggggccgatg acgccgtggt tgccctcgga gtcgcggtgt
      661 tcgagttcgc cgtcgagcac ccatgtcacg atttccatat cggcgtgggg gtgggagccg
      721 aatcctccgc cgggtgcgac gacgtcgtcg ttggagacga tcagcagccc gtggccgagt
      781 tcgttggggt tgaagtggtg gccgaaactg aacgagtgcc acgagtcgag ccagccgata
      841 tcggtgtgaa accgttggcg tgcacgacgt atgtcggctc gggtcgaggt tgtcgtcgtc
      901 atggtggtca cgctccttgc acgctctgcc tgcaggttcc accggtacaa actatttgca
      961 gatgcaaata attcccggac cgcagccgcg agggttcgca gcgcgtcagg cgctcgggag
     1021 actggtgctg tacacccacg cgtcgaagaa cgcaccgagg tcgcgccccg cgattttcga
     1081 tgcatatgcg acgaagtcgg aggtcgtgac cgaggcgtcg gcgaattctg cgggccaccc
     1141 tgcgatgatc gttgaaaaag cctcctcgcc aacctctgcg cgaagcgcgt ggagggcgag
     1201 cgccccacct tcgtagcgca gcggccccca cagatcgtgc gggtccgggt cggcgatggc
     1261 atccatggag accccggacg agtcgaagcc ggcgcggatg gcgtcggcct gcgactgcac
     1321 cgaggcgcct ccactcgcag cggaccacat ccacgacaga tacgtcgccc aaccctcgct
     1381 caaccacagg tccgaccagg agccgaggtt gaccaggttg ccgaaccatt gatgcgcgag
     1441 ttcgtgcgcg gtcacctccg cgacgagttc agtggatccg gaggtgaacc agtcgagcgg
     1501 gatcatcgac agggtgtggg tctcgagcgc gaacgcgagc ggcgtgtcgg gaacgaacac
     1561 gccgtagatg ccctccggca ccgggccgac ggtcgcatcg aaccagcgga gctgtgcctc
     1621 ggtgagcccg agttgctccg tcaccgcagc gggtgcgctg cggggcacgg cgtggcggat
     1681 ggtcaccgtc gactgcgaga gcgccacgtc gtgggtggtc acctcgtagt cgcccaccgc
     1741 catctgcagc gcgtacagcg gaatgtcgtc gctcaccgtg aactgcgagg tggtcgagcc
     1801 gtcggggtgg gtgatgacct cgccggagcg tccggaggcc accgcagttt cccccgctgc
     1861 ggcgtgaatc gtgatgtgga actgcgcggg atcgctcggg tggtcgttgc acgggatgat
     1921 gccgtgcatg ccgttgggtt cggcggcggt gaacgacccg gtcggcgacc gcagccaccc
     1981 gatggaaccg ggggacgtga cgtcgtcgat cgtcgacggg gtgccggaat aggtaatggt
     2041 cacgacgtgg tccccggaga tcggcgacgg cggggtgacc accaactccc cgccgtcgcg
     2101 agccaccgtc gcggcggagc cgtcgacgag cacctcatcg atctcaaatc cggcgaagtc
     2161 gaggttgaag ctggccagcg gggcgtcggc ggtcgtcgag agttcgatac cgacggtggc
     2221 agccaccgtg ccgccgcggt tggcgtcggt cgcgtcgaag tcgaccacat atcgggtgac
     2281 gtcgtagccg ccgttgccgg agcccgccac gatcggatca tcgagcgatg ctgagccgtt
     2341 catcgtcgca acgctcgtgg tggtcgcggt cgtcgtggtg gccgaagcgg cgtcgtcgac
     2401 gacggaggtg gcctcgccgg acccgccggc gctacacgat gagaccgcag cgaccaagga
     2461 gatggcggcc accgtgcccg ccaccgtgga gagtctggcg aaggttcgcg gagcactcat
     2521 cgaggggaca acgtacacgt gacatcgccc gcaccggtcg gcagcgtcga cgccggtccg
     2581 ctggcaggcg atggttggaa accgaggtta cccaccggta acttataggc cagcggcgag
     2641 ctcgccgtgg tgttcccgtg tggcccgaat ggccattcga cgcagagaag gtgaccgaca
     2701 catgagcaac ttttcgctcg cactcaacga cgaccaactg cagctccaag agtgggtcca
     2761 cggtttcgcc aaggacgtga ttcgtcccgc cggcgaggaa tgggacgaaa aagaggagtt
     2821 cccctggccc atcgtgcagg aggcggcgaa gatcggcctg tacgggatgg acttcatggc
     2881 gaacgccctg ttcgacccca ccgggttgtc gatggtgctc gccgtcgagg agctgttctg
     2941 gggcgacgcc ggcattgcgc tgtcgatcat gggatcgggt cttgccgccg cggcgatctc
     3001 ggccaacgga acccaggaac agacccttga atgggttccg cagtgctacg gaaccgccga
     3061 aaaggtgcag ctcggtgcgc tgtgtgcctc cgaacccgac gcaggctcgg acgtcggcgg
     3121 ataccgcacc cgcgccgtct acgacgaaaa gaccgatacc tggaccttga acggcaccaa
     3181 ggcgtggatc accaacggtg gcatcgccga cattcacgtc atcgtggcgg tcgtcgagcc
     3241 gggcatcggt tcgcggggcc atgccagc
//
LOCUS       DAOOMA010000277         3262 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_35749_length_3262_cov_4.547552, whole genome shotgun sequence.
ACCESSION   DAOOMA010000277 DAOOMA010000000
VERSION     DAOOMA010000277.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 3262)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3262)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3262
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35749_length_3262_cov_4.547552"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..771)
                     /locus_tag="PLP95_08145"
     CDS             complement(<1..771)
                     /locus_tag="PLP95_08145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HPB45807.1"
                     /translation="MTNILTESAKRRTFAIISHPDAGKTTLTEKFLLYGGAINQEAGT
                     VKARGDRRSATSDWMALEQQRGISISSTVLQFPYRGCTVNLLDTPGHRDFSEDTYRVL
                     TAADAAVMVLDAAKGIEPQTLKLFEVCRDRGLPLLTFINKWDRPGRDGLELLDEIETM
                     IGVQPTPVTWPVGIPGDFRGVVDRRSGEYTRFTRTARGATIAPEELVDPERAAIEEGS
                     AWEACMEELELLEAAGQDFDDETFLAGMTTPLFVGSAMT"
     gene            complement(887..1219)
                     /locus_tag="PLP95_08150"
     CDS             complement(887..1219)
                     /locus_tag="PLP95_08150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB45808.1"
                     /translation="MGTPVVSGPDGIYVSNYASHRARVPLNGAKVGGTVWVFFDEPGV
                     SAVRYWLNDPSGYGTPVIETTAPFDLAADGFAFSTLEPGSHTLLVEATTSAGTIRRLA
                     TFSVIAID"
     gene            1212..1472
                     /locus_tag="PLP95_08155"
     CDS             1212..1472
                     /locus_tag="PLP95_08155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB45809.1"
                     /translation="MPTGAVVVVAAGRLVVVGEFLRGAVVEDVLDGGSRGVVTSGSVA
                     GMLGDGWVVVVVVVGVVVARGSDGGGATSLGAAMSAFRALWA"
     gene            complement(1649..2284)
                     /locus_tag="PLP95_08160"
     CDS             complement(1649..2284)
                     /locus_tag="PLP95_08160"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HPB45810.1"
                     /translation="MLRILLADDHQILREGIRRGLEAAGEEVIGEAGNGEEAVELART
                     LLPDVVLMDLSMPVMDGIEATRRITTELPEVKVLVLTMHDDPARTRGAISAGASGYLT
                     KGTSFAEVLNTIRIVAAGETFLSPALAVSMLRAVESASVTDDLLSDRQVEILQMVANG
                     HGTKQVARELGITQKTVHNHLNAIYRRLDTQSLTHAVLSAVRLGIIQLDQG"
     gene            complement(2262..>3262)
                     /locus_tag="PLP95_08165"
     CDS             complement(2262..>3262)
                     /locus_tag="PLP95_08165"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HPB45811.1"
                     /translation="FKYDDPSEEWVPKLSEGATVETAYRLHTLPAPLRRALESRGPCL
                     VGDLRLGDTPGINRHSGSGLYTALVARGRTVGLLALEHPTPGQYSESDRIVLAGMADI
                     VALTLDNARWFGRLRTLGAEEERVRIARDLHDRLGQWLTYISFELERYIDDHGPSDPQ
                     LEQLHGDVQTALDELRETLRQLRSTVTRERPFSEVAAEHIERFAERTGLEVEFTVEHP
                     RSRLSVPVENELLRILQETLNNIDKHAQAGNVSVTWNVNGPTASLTVTDDGRGFDPSK
                     GVRESAYGLVGIRERADVIGARISVDSAPDHGTTIHVEVGTTPARKEDTHAQNLVG"
BASE COUNT          528 a         1005 c         1116 g          613 t
ORIGIN      
        1 cgtcatcgcg gatccgacga acagcggggt cgtcattccg gcgaggaacg tctcgtcgtc
       61 gaagtcctgc cccgcggctt caaggagttc gagttcctcc atacatgcct cccacgccga
      121 accctcttcg atagcggcgc gctcgggatc gacgagctcc tcgggggcga tggtcgcgcc
      181 gcgcgcggtg cgggtgaaac gggtgtactc gccgcttctg cggtcgacga caccacgaaa
      241 gtcgccggga atcccgaccg gccaggtgac cggcgtcggc tgaactccga tcatcgtctc
      301 gatctcgtcg agcaactcca ggccgtcgcg ccccggacgg tcccacttgt tgatgaaggt
      361 caacagcgga aggccacggt cgcgacagac ctcgaacagc ttgagcgtct ggggctcgat
      421 gccctttgcc gcgtcgagca ccatcaccgc ggcgtcggcc gcggtcaaca cccggtaggt
      481 gtcctcggag aagtcgcggt gcccgggggt gtcgagcagg ttcacggtgc atccgcggta
      541 cgggaactgc aacacggtcg acgagatgga gatgccgcgc tgttgctcca gcgccatcca
      601 gtcggaggtg gcgctacggc ggtcgcctcg tgccttgacc gtgcccgcct cctgattgat
      661 cgcaccgcca tacaggagaa acttctcggt caacgtggtc ttgccggcgt cggggtggga
      721 gatgatggcg aaggtccgcc gcttggccga ctcggtaagg atgttggtca cgctcgtgcc
      781 tgttcgatcg agaaggtgcc gacgcgtcga aaatcggccc gctccatagt ggccgaacga
      841 ccgcggcaac ccgaagttcg cggcgacggg tcgaggtggt cgaccgtcag tcgatggcga
      901 tcacgctgaa cgtggcaaga cgccggatgg tcccggccga ggttgtggcc tcgaccagca
      961 gggtgtgcga accgggctcc agcgtcgaga aggcgaagcc gtcggcggcg aggtcgaacg
     1021 gtgcggtggt ctcgatgacc ggtgttccgt acccgctcgg gtcgttcaac caataccgaa
     1081 cagcgctgac gccgggctcg tcgaagaaca cccagacggt gccgcccacc ttggctccgt
     1141 tcagcgggac acgggctcga tggctggcgt aattcgacac gtagattccg tcgggccccg
     1201 acaccaccgg cgtgcccacg ggggccgtgg tggtcgtcgc ggcaggccga ctcgtcgtgg
     1261 tcggcgaatt cctgcgtggg gcagtcgtgg aggatgtgct cgacggcggg tcgaggggcg
     1321 ttgtcacctc gggttcggtg gcaggcatgc tcggtgacgg ttgggtcgtc gtggtcgtcg
     1381 ttgtcggcgt ggtcgtcgca cgtggctcgg acggcggtgg cgcgacatcg ctcggggccg
     1441 cgatgtcagc gtttcgggcc ttgtgggctt gataggcgag cggaccagac accgccgtgg
     1501 tcaccgacgt tgacacgacg agcgcgagaa cgacgcggaa tgtcaacatt ccgctcagga
     1561 ttcccatcca agttttgatc ttggcgttca tcgttcagtc atcgtcaaag tcggtcgctc
     1621 gaaggggtcc ggcgacgggt ggtggcatct agccctggtc gagttggatg atcccgaggc
     1681 gcaccgcgct gagcacggcg tgggtcaggc tctgggtgtc gagtcgccgg tagatcgcgt
     1741 tcagatggtt gtgaaccgtc ttttgcgtaa tacccaattc gcgtgcgacc tgcttggttc
     1801 cgtggccgtt cgcgaccatc tggaggattt cgacctgccg gtcgctcagg agatcgtcgg
     1861 tgacggaggc ggattcgacc gctcgcagca tggacacggc gagggccggg ctgagaaacg
     1921 tctcgccggc agcgacgata cggatggtgt tgagtacctc ggcaaaggag gttcccttcg
     1981 tgagataccc cgaagcgccg gcggagatcg ccccacgggt gcgggccggg tcgtcgtgca
     2041 tcgtcaacac caacaccttg acctcgggca actccgtggt gattcggcgg gtcgcctcaa
     2101 tgccatccat gacgggcatc gacaggtcca tcaagacgac gtccggcaac aaggtgcgtg
     2161 cgagttcgac cgcttcctct ccgttgcccg cctcgccgat gacctcttcg ccggcggctt
     2221 cgagcccccg ccgaatgccc tcacggagga tctgatggtc atcagccaac aagattctga
     2281 gcatgtgtgt cttccttcct cgccggggtg gtgccgactt caacatgaat ggttgttccg
     2341 tggtccgggg cgctgtcgac cgagatgcgc gcaccgatga catcggcgcg ttcgcgtatt
     2401 ccgacgagcc cataggcgct ctctcgcacc cccttggacg gatcgaaccc gcggccgtcg
     2461 tcggtgaccg tcagcgaagc cgtcggaccg ttgacgttcc atgtcaccga aacgttgccc
     2521 gcttgggcgt gcttgtcgat gttgttcaac gtctcctgca ggattcgcag gagttcgttc
     2581 tccaccggca ccgagagccg cgatcgtggg tgttcgacgg tgaactcgac ctcaaggcca
     2641 gtgcgttcag cgaaacgctc gatgtgttcg gcggccacct cggaaaacgg tcgctcccgc
     2701 gtcacggtgg agcgaagctg gcgcagggtc tctcgtagtt cgtcgagcgc ggtctggacg
     2761 tctccgtgga gttgttcgag ctgcggatcg ctcgggccgt gatcgtcgat gtagcgctcg
     2821 agttcgaaac tgatgtaggt gagccactga cccagccggt cgtggaggtc tcgagcgatc
     2881 cggacccgtt cctcctcggc cccaagcgtc cggagccgcc cgaaccagcg cgcattgtcg
     2941 agggtgaggg ccacgatgtc ggccatcccc gccaggacga tccggtcgct ttcggagtac
     3001 tgcccgggcg tcggatgttc gagcgccaac aggcccacgg tgcgaccacg cgcaaccaac
     3061 gcggtgtaca gacccgaacc ggagtggcga ttgattccgg gagtgtcacc cagtcggagg
     3121 tcgccgacga gacacggtcc ccggctctcg agtgcccgac gtaacggtgc gggcagggtg
     3181 tggagccgat acgcggtttc gacggtggcg ccttcggaga gtttcggcac ccattcctcg
     3241 gaggggtcgt catatttgaa ga
//
LOCUS       DAOOMA010000278         3262 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_35750_length_3262_cov_3.999065, whole genome shotgun sequence.
ACCESSION   DAOOMA010000278 DAOOMA010000000
VERSION     DAOOMA010000278.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 3262)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3262)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3262
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35750_length_3262_cov_3.999065"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(161..1975)
                     /locus_tag="PLP95_08170"
     CDS             complement(161..1975)
                     /locus_tag="PLP95_08170"
                     /inference="COORDINATES: protein motif:HMM:NF015557.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HPB45812.1"
                     /translation="MTDAAVALLVLGGVIALFMANRFPVGSVAVATSLTLWATGLLTF
                     DEAVAGFGDPVVIFIATLFIVSEAIDANGLTTWAGQRVVAIAGDNMSRVLVSIMALCA
                     VLTALISLNGSVAALLPMAVMLAMKLKVPPSRLLMPMVYAGSAGSMLVLMGSPVNVIV
                     SDAASEVGEGAFGFFEFAVLGVPLLIATMLIGALLTRRLVPDRQPVSAPPDLSRHAHA
                     LASHYQLSEGFYRLRVRDKSPLIGRKVASLRFDDHPTIDLIATQGADGLARAVEHVLE
                     PDDVLVVSGTTEHVSTLAVADGLAVALSPASPDSPDTLIGREAGVAEVVVPPRSPLID
                     ETMFPGMVRGDDLVILAIQRYGRDFGDKPVKIAAGDSILVWGSWDSVDGLVTDRDVLI
                     VDSPDLMRRQAGPLGKAATRTAIIVGAMVLSLAFGLVPPAGAGLIAALALVLSGSISS
                     AQAYRAVSWETVVLVGGLIPMSAAIQSSGAADSIAERVIDLVGSGSPMLLMLALFALT
                     AVLGLVISNTATVLIVLPVALSVAAETGYAAKPVLMLIAVASSAAVLTPVQTPGNMMV
                     MAPAGYRFSDYWRLGAPVLLAWLAIALVVIPIMWPFAP"
     gene            complement(1972..3105)
                     /locus_tag="PLP95_08175"
     CDS             complement(1972..3105)
                     /locus_tag="PLP95_08175"
                     /inference="COORDINATES: protein motif:HMM:NF013740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HPB45813.1"
                     /translation="MADTSADCTTTSPPAAPRPRFESPTLNSVGRSAWAVVGITLVAA
                     GAIYGLAAISTLVLPLVFAAVFAILAQPMQRWLLDRRIPPGLAAGLIVFAIAATIIGV
                     IALTARGLIEQYDRIADEVESALTHFDIDNADIEAIRNQLQNLGSGLGSGAAGSVAAG
                     FSAVGRTLVGVLLGGLIFYYFLKDGSRIRRGAVNRAAADQQAHLNAFIEDTFFVFRRY
                     WLGRAIISAVVAAVVGIGAAILNLPLITTIVVVTFVGGFIPYLGALIGGALTVVVALG
                     SNGIVSAIVMLGVVMVANIVIENLVEPAVTGKTLQIHPLVVLLSTTLGATVAGIPGMI
                     MAVPIVVIAQRAVPVYRTVVLGHPFVDVPLDLDPLDDEPQKQP"
BASE COUNT          629 a         1032 c         1131 g          470 t
ORIGIN      
        1 gctcggcggt gccgcgatcg ccgcggcgtc gtgggcatcc cgatgacccc cgctacgcac
       61 ccgccacatg gtcatcggtt ccaaccgagt ccatcaccgg agaacccatc atgagtctgt
      121 tcaagaccgc cggccggacc gctgcggtcg gcggggtcgc tcagggcgcg aacggccaca
      181 tgatcgggat cacgaccaat gcgatcgcca gccatgcgag cagaaccggt gcgccgaggc
      241 gccaatagtc gctgaaccgg taacccgccg gggccatcac catcatgttg cccggggtct
      301 gaaccggggt caggaccgca gcggaactcg cgacggcgat caacatgagc acgggcttgg
      361 cggcgtaccc ggtctcggcc gcgacggaca aggccaccgg caagacgatg agcacggtgg
      421 cggtgttgct gatgacaagg ccgagcaccg cggtgagcgc gaagagggcg agcatcaaca
      481 gcatcggcga acccgaaccc acgaggtcga tgacacgctc ggcgatgctg tcagcggcac
      541 cacttgattg gatggccgcc gacatcggaa tgagtccgcc gaccagcacg acggtttccc
      601 acgacaccgc gcggtaggcc tgtgccgagg agatcgaacc gctcaacacg agggccagtg
      661 ctgcgatcaa gccggcgccg gcagggggaa cgagaccgaa ggcgagcgac agcaccatgg
      721 cgccgacgat gattgcggtg cgggtcgcgg ccttgccgag cggtcctgcc tggcggcgca
      781 tgaggtccgg gctgtcgacg atcaagacgt cgcggtcggt gacgaggccg tcgaccgaat
      841 cccaagaacc ccacacgagg atcgagtcgc ccgcagcgat cttgaccggc ttgtcgccga
      901 agtctcggcc gtaccgctgg atcgccagga tcacgaggtc gtcgcctcgg accatgccag
      961 ggaacatcgt ctcgtcgatc agcggtgacc tcggcggcac cacaacctcg gccacgccgg
     1021 cctcgcggcc gatgagggtg tctggggagt ccggcgacgc ggggctgagt gcgaccgcaa
     1081 gcccatcggc gaccgccaac gtgctgacgt gttcggtggt ccccgagacg accagcacgt
     1141 cgtcgggttc gagaacatgt tccacagcgc gggcaaggcc gtcggcccct tgtgtggcga
     1201 tgaggtcgat cgtggggtga tcgtcgaaac gcaacgaggc gaccttgcgg ccgatcagcg
     1261 gcgacttgtc gcgaactcga agccggtaga aaccctcgct gagttgatag tgggaggcga
     1321 gcgcgtgggc gtgccggctg aggtcgggtg gggcgctgac gggctgacga tccggcacaa
     1381 gacggcgtgt cagcagggcg ccgatgagca tcgtggcgat caggaggggt acgccgagga
     1441 cggcgaactc gaagaacccg aaggcgcctt cgccgacctc gctggcggca tcggacacga
     1501 tgacgttgac cgggctgccc atcagaacca gcatcgaacc ggcgctaccg gcgtacacca
     1561 tcggcatcaa tagacgtgac ggtggaacct tcagcttcat ggccaacatg accgccatcg
     1621 gcaacagcgc tgccaccgat ccgttgagac tgatcagcgc tgtgagcaca gcgcacaacg
     1681 ccatgatcga caccagcacc cgcgacatgt tgtcgccggc gatcgcgacg acccgttgcc
     1741 cagcccaggt ggtgaggccg ttggcgtcga tggcttcgct gacgatgaac agggtggcga
     1801 tgaagatcac cacggggtcg ccgaacccgg cgacggcctc gtcgaaggtc aacaggcccg
     1861 tggcccacag cgtgagcgag gtggcgacgg cgacggagcc cacggggaac cggttcgcca
     1921 tgaacaacgc gatgaccccg ccgaggacga gcagcgcgac agccgcgtcg gtcatggttg
     1981 cttctggggt tcgtcgtcga gtgggtccag atcgagcggc acgtccacga aagggtggcc
     2041 gagaacgacg gtgcgataca caggaacggc ccgctgagcg atcaccacga tgggaacggc
     2101 catgatcatg ccgggaatgc cggccacggt cgcgccgagg gtggtggaca acaggacgac
     2161 gagcgggtgg atctgaagcg tcttgccggt gacggcgggt tcgacgagat tctcgatgac
     2221 gatgttggcg accatgacca caccgagcat gacgatcgcc gacacgatgc cgttcgaacc
     2281 cagcgcgacg acgacggtga gcgcgccccc gatcagcgct cccagatacg ggatgaaccc
     2341 gccgacgaag gtgacgacga cgatcgtggt gatcaacgga aggttcagga ttgcggcacc
     2401 gattccgacc acggcagcga cgacggccga gatgatggcc cgcccgagcc agtagcggcg
     2461 aaagacgaag aacgtgtcct cgatgaacgc attgaggtgg gcctgctgat ctgccgcggc
     2521 gcggttgacc gcgcctcggc ggattctcga gccgtccttg aggaagtagt agaagatcag
     2581 cccgcccaac aacaccccga ccagggtccg gccgacggcg ctgaaccctg ctgcaaccga
     2641 tcctgcggcc ccggacccca gcccgcttcc gaggttctgc agttggttgc ggatcgcctc
     2701 gatatcggcg ttgtcgatgt cgaaatgcgt cagagcggat tcgacctcat ccgcgatccg
     2761 gtcgtactgc tcgatgaggc cccgggcggt gagcgctatc acgccgatga tggtcgcggc
     2821 gatggcgaac acgatgagtc cggcagcgag ccccggtggg atccggcggt cgagcagcca
     2881 gcgctgcatc ggctgggcga ggatcgcaaa gacggcggca aacaccaggg gaagtaccag
     2941 cgtgctgatc gcggcgagtc cgtagatcgc ccccgctgcg acgagggtga ttccgaccac
     3001 tgcccaggcg ctgcgtccaa cactgttgag tgtcggactc tcgaagcggg gtcgtggggc
     3061 ggcaggtggg ctcgtcgtcg tgcaatccgc gctcgtgtcg gccatgaggg caggttaggg
     3121 ggcctgggta tgcacccgct gccgtccaca gaactgccgt ccacagaact gccgtccaca
     3181 gagtcagcac cctggggcta gtgtcgaccc atgtcagccg ccgattccca cgcctctgat
     3241 ggattgggtc ccggcggcgt cg
//
LOCUS       DAOOMA010000279         3259 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_35788_length_3259_cov_3.629213, whole genome shotgun sequence.
ACCESSION   DAOOMA010000279 DAOOMA010000000
VERSION     DAOOMA010000279.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 3259)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3259)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3259
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35788_length_3259_cov_3.629213"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..878
                     /locus_tag="PLP95_08180"
     CDS             <1..878
                     /locus_tag="PLP95_08180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB45814.1"
                     /translation="DDRRKIGPLLRDTQRIDHKSTSSTLEAEVLEMRLIHHFEPAYNR
                     DGTRWRSSVYVKVDSGRSSAGSSPSGSRQPRLIVTAAPKPDDRVVAGPLSSRRIARSF
                     VHAVEQVADRVAEPELNLTTLCEDPTVVVDAFTQRMSELAAEERFEAAAAARDHLGDV
                     ASTVERQRLLDSMARCTIEFATPDGHRFLVRRGLLERVWEPSQRAGIDGLDPGRAIEA
                     MPTGPGDADRPLRADLADEVLLIGRWLERNSERIRLLRVDGLLASPRTPVPSPGRAPE
                     QSQPEQSQPEQSQPA"
     gene            complement(898..1953)
                     /gene="trpD"
                     /locus_tag="PLP95_08185"
     CDS             complement(898..1953)
                     /gene="trpD"
                     /locus_tag="PLP95_08185"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: protein motif:HMM:TIGR01245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HPB45815.1"
                     /translation="MTAGEFPGWPALLGHLADHEDLSEQQAQAAMSQILAGSATDAQI
                     AAFIVGLRIKGETAEEILGLSDAMLEVAAPLNLADGTVDIVGTGGSHTLRNRAFNVST
                     ISSIVAAAAGATVCKHGNRKASSTSGSVDVLEQLGVAVELDGPGVERCIAEANVGFAF
                     ARVFHPAMRFVAGVRSELGIPTVFNILGPLSHPGRVRRQLLGVADRSRMDIVAEVLAA
                     RGVDRALVVHGLDGLDEITTTGHTTVIEIRGHTIERYDVTPTDLGLPVRTVDEVAVGD
                     PAQNAAVARDMLAGKPGAHREIVTLNAGAALYVSDLATSIAEGVAKASEALDSGAAAA
                     TLDRLVAASQAAASNAG"
     gene            complement(1953..2207)
                     /locus_tag="PLP95_08190"
     CDS             complement(1953..2207)
                     /locus_tag="PLP95_08190"
                     /inference="COORDINATES: protein motif:HMM:NF014520.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WhiB family transcriptional regulator"
                     /protein_id="HPB45816.1"
                     /translation="MDRNWVGRAACSGLDPAVFYPPTDDEAVEAKAICEQCPVQQECL
                     EHAIEHREKNGVWGGATERERLRIIRRRRRLRSQTASIAS"
     gene            2382..>3259
                     /locus_tag="PLP95_08195"
     CDS             2382..>3259
                     /locus_tag="PLP95_08195"
                     /inference="COORDINATES: protein motif:HMM:NF013434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HPB45817.1"
                     /translation="MTFASFERSTALTRLRDESFDLVVIGGGITGAGVALDAASRGLK
                     VAMVERDDFASGTSSKSSKLIHGGLRYLQQGDVALVYEALAERQTLLNNAPHLVELLP
                     FLIPMVGEGGVLPAKASRLLGGAMWGYDLTGGFRIGKLHERLTKAEALAYMPTLPADN
                     LVSAYLYYDATADDARLVLTVARTAALHFGAVVANGVEVAGLRHNRLGLVDGVEVSAD
                     GEHFTIDTASVVNAAGVWSDEVRCFDEGTDPDSMRPAKGVHIAIPHYLVQNEVAAVIP
                     VPGDKRTVFVVPNGEFT"
BASE COUNT          566 a         1125 c         1041 g          527 t
ORIGIN      
        1 cggacgaccg ccgcaagatc ggcccactcc tgcgcgacac ccagcgcatc gaccacaagt
       61 ccacctcgtc aacgctcgag gccgaggtgt tggagatgcg cctcattcac catttcgaac
      121 ccgcttataa ccgcgacggg acccgctggc gctcgtcggt ctatgtgaag gtcgactccg
      181 gacgctcgtc cgccggaagc tcaccctccg ggagccgcca gccgcgcctc atcgtcaccg
      241 ccgcccccaa acccgacgac cgggtcgtcg ccgggccgct gtcgagccgc cgcatcgctc
      301 gatcctttgt ccacgccgtc gagcaggtgg ccgatcgagt cgccgaaccc gaactcaacc
      361 tgacgaccct ctgcgaggac ccgacggtcg tggtcgatgc gttcacacaa cgcatgagcg
      421 aactcgcagc cgaggaacgc ttcgaagcgg ccgcggcggc gcgggatcat ctgggtgacg
      481 tcgcctccac cgtcgaacgc caacgcctcc tcgactcgat ggcgcgatgc accatcgagt
      541 tcgcaacccc cgacgggcac cggtttctgg tccgccgggg actcctcgaa cgggtttggg
      601 agccatcgca gcgagcgggc atcgacggcc tcgatccggg acgggccatt gaagcgatgc
      661 cgacgggacc gggcgacgcc gaccgcccac tgcgagccga cctggcggac gaggtgttgc
      721 tgatcggccg gtggctcgaa cgtaacagcg aacggatccg actactccgg gtggacgggc
      781 ttctcgcgtc gccacggacg ccggtcccct cacccggtcg agcgcccgaa cagagccagc
      841 ccgaacagag ccagcccgaa cagagccagc cggcctgagg ttggggcgaa ccgaggttca
      901 gcctgcgttc gacgcggcag cctgcgacgc ggcgacgagc cggtcgagcg tcgctgccgc
      961 agcaccggag tcgagtgcct cggaggcctt ggccaccccc tcggcgatcg aggttgcgag
     1021 atcgctcacg tacagggcgg caccggcgtt gagcgtgaca atctcgcggt gagcacccgg
     1081 cttgcctgcg agcatgtcgc gggcgaccgc ggcgttctga gcgggatcgc cgaccgccac
     1141 ctcgtcgacc gtgcgaacgg gcagacccag atcggtcggg gtgacgtcat agcgctcgat
     1201 ggtgtgaccg cggatctcga tcacggtcgt gtgaccggtg gtcgtgatct cgtcgagacc
     1261 gtcgaggccg tgcacgacaa gggcccgatc gacgccccgg gcggccaaca cctcggcgac
     1321 gatgtccatg cgcgagcggt ccgccacgcc gagcaactgc cgccggacac gacccgggtg
     1381 cgacagcgga ccgagaatgt tgaacaccgt cgggatgccg agttcgcttc gcaccccggc
     1441 gacgaagcgc atcgcggggt gaaagacccg ggcgaacgcg aatccgacgt tcgcctcggc
     1501 gatgcaacgc tcgacccccg ggccgtcgag ttcgaccgcg acgccgagtt gttcgagaac
     1561 atcgacgctt ccggaggttg aggaggcctt ccggttgccg tgcttgcaca ccgtcgcccc
     1621 ggcggccgca gcgacgatgc tcgagatggt cgagacgttg aaggcgcggt ttcgcagcgt
     1681 gtgggacccg cccgtgccaa cgatgtcgac ggtgccatcg gccagattca gcggggccgc
     1741 gacctccaac atggcatcgg acagcccgag gatctcctcg gcggtctcgc ccttgatccg
     1801 aagtccgacg atgaacgctg cgatctgggc gtcggtggcg gaaccggcga ggatctgcga
     1861 catcgcggcc tgcgcctgtt gttcggaaag gtcctcgtga tccgccagat ggcccagcag
     1921 tgccggccac cccgggaact caccggccgt catcaggaag cgatactcgc cgtctgagag
     1981 cggaggcgac gtcgccgacg gatgatccgc aaccgctccc gttcggtcgc accaccccac
     2041 acaccgttct tctcacggtg ttctatcgca tgctcgaggc attcctgctg aacggggcac
     2101 tgctcgcaga tcgccttggc ctcaacggct tcgtcatcgg tgggtgggta aaagacggcg
     2161 ggatcgagtc cgctgcacgc ggctcgccca acccagtttc tgtccattgg ccttcgatcc
     2221 tcgcggttgt atcggtgcgc tgtccaattg attctcagga ttcaaaacac cccaaaaccc
     2281 gggtcggatt cccaccaaca gaacaacgtg acatcggtca cattttggag ggaagtcgac
     2341 ccggttaccg atcggtaact tccgggttta gcatggagtg cgtgactttt gcatcgttcg
     2401 aacgctcgac cgcactgacc cgtcttcgag acgagtcctt cgatctcgtg gtcatcggtg
     2461 gcggcatcac cggcgccggc gtcgccctcg acgctgcgtc gcgaggcctc aaagtcgcca
     2521 tggttgaacg cgacgacttc gcctcgggca cgtcgtcgaa atcctccaaa ctcatccacg
     2581 gcggattgcg ctatctccaa cagggcgatg tcgcgctcgt ctatgaggcg ctcgctgaac
     2641 gacagacgct gttgaacaac gccccacacc tcgtcgaact cctcccattt ctcattccga
     2701 tggtcggcga gggcggcgtc ttgccggcca aagcatcacg actgctgggc ggagccatgt
     2761 gggggtacga cctgaccggc ggattccgga tcggcaagct gcacgagcgg ctcaccaagg
     2821 ccgaagcgct cgcctacatg ccgacactgc cggccgacaa tctcgtcagc gcctacctct
     2881 actacgacgc caccgcggat gacgcccgcc tggtcctgac cgtcgcccgc accgcggcgc
     2941 tgcatttcgg cgcggtcgtc gccaacggag tcgaggtggc ggggttacgc cacaatcggc
     3001 tcggcctcgt cgacggggtc gaggtgagcg cagacggtga acatttcacg atcgacacgg
     3061 cgtcggtcgt caacgccgcc ggagtgtggt ccgacgaggt ccggtgcttc gacgagggca
     3121 ccgaccccga ctcgatgcgg ccggccaaag gggtgcacat cgccatcccc cactatctcg
     3181 tgcagaacga ggtcgctgcg gtcatccccg ttcccggaga caagcgcacc gtgttcgtgg
     3241 tgcccaacgg cgagttcac
//
LOCUS       DAOOMA010000280         3258 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_35808_length_3258_cov_4.131751, whole genome shotgun sequence.
ACCESSION   DAOOMA010000280 DAOOMA010000000
VERSION     DAOOMA010000280.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 3258)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3258)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3258
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35808_length_3258_cov_4.131751"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..600)
                     /locus_tag="PLP95_08200"
     CDS             complement(<1..600)
                     /locus_tag="PLP95_08200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012223166.1"
                     /note="activates fatty acids by binding to coenzyme A;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HPB45818.1"
                     /translation="MELSSRTLSATTERLAAMARNALEVARFGGLDTGEESSAYTVVH
                     ETPLYRLRRYHADRDSGPKAKPRPSVVLVPPMMLSADVFDVSPVTSAVSLLAGDGIDP
                     WVIDFGAPEHEQGGMQRTLTDHVLAISDAVEFLNGYLGAEVHLGGYSQGGMFCYQAAA
                     YRRSEGIASIVTFGSPVDLHGMIPFGIPDEVALRVLEFVG"
     gene            complement(678..1382)
                     /locus_tag="PLP95_08205"
     CDS             complement(678..1382)
                     /locus_tag="PLP95_08205"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012345.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HPB45819.1"
                     /translation="MRAVVIQHLDVEGPAHIATALDAAGVEVDVIRADLGQRIPEDLD
                     GIDGLVVMGGPMSARSDDGFPTRGAEIALLRQALDREVPTLGVCLGAQLLAAAAGARV
                     IAGHGLEVGWGAVEVLECAVDDDLFFGLDGHLDVLHWHGETYSLPAGAIHLARSQAYE
                     QQAFRVGRCTWGLQFHLEVGVDEATRFIDSFPDEADAALGGRHGVREGCGPAVEALAP
                     VRDLVVARFAALVARR"
     gene            complement(1389..2420)
                     /locus_tag="PLP95_08210"
     CDS             complement(1389..2420)
                     /locus_tag="PLP95_08210"
                     /inference="COORDINATES: protein motif:HMM:NF013862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HPB45820.1"
                     /translation="MKCSLNLGAFTSSGWGRPHLTREPEAGSDAPEAAPEPSAKRVLV
                     LSGGGIFGAAQAGMLQSLAEHSNWRPDVIVGTSAGALNGSWVAHDFTADATKALSDIW
                     SHFDSRGVFPQGVLSQALNLVRRRPSVQDGAKLRSLISDLCPIERLEDLRIPLHVSAT
                     NILTGLPHWFDAGFARDVLYASAALPAIVPPAVIDGMSFVDGGVVANLPVQRAAELGG
                     DSILCLDVSAAGNDSVPRTALGMLTRSFSIALNAATDAIVDAASIDRQLWRVRPTIDP
                     SLNTSDWRACSRLVEAGAESMKVWLDHNAEQLEPVSEPSPAAASGPVIDLRRHEHPVF
                     QRFARRAVS"
     gene            complement(2565..3014)
                     /locus_tag="PLP95_08215"
     CDS             complement(2565..3014)
                     /locus_tag="PLP95_08215"
                     /inference="COORDINATES: protein motif:HMM:TIGR00028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HPB45821.1"
                     /translation="MIVDANVLIYAVDEQSHFHEVARSWLEDALNGNERVGLPWVSLM
                     AFQRIMTHPRVSANPLSPRDAWGFVSDWLDIAQVWVPAAGQRHRDILKRLLIDGDLRA
                     NLVTDAHLAALAIEHGTSICSFDSDFVRFGGLRWINPTDLAGGFGRS"
     gene            complement(3011..>3258)
                     /locus_tag="PLP95_08220"
     CDS             complement(3011..>3258)
                     /locus_tag="PLP95_08220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB45822.1"
                     /translation="PMRTTVRLDPEVVAAAEQLRRERNIGLGEAVNELARAGLARELP
                     PTKFQQRTASVGLRIDVTDIADALELLDEVVADGSER"
BASE COUNT          602 a         1097 c         1057 g          502 t
ORIGIN      
        1 acccacgaat tccagcactc gtagtgccac ctcatctggg atgccgaacg ggatcatccc
       61 atgcaggtcg accgggcttc cgaacgtgac gatgctggcg atcccctccg acctgcgata
      121 cgccgccgcc tgataacaga acatgccgcc ctgggaatag ccgccgagat gaacctcggc
      181 accgaggtac ccgttcagga actcgacggc gtcggagatg gcaagcacat gatcggtgag
      241 ggtgcgctgc atgccaccct gctcgtgttc cggagcgccg aagtcgatca cccaggggtc
      301 gattccgtca ccggccagga gcgaaaccgc gctcgtcacc gggctcacat cgaacacgtc
      361 ggccgacagc atcatcggag gcacgagcac gaccgacggc cgtggtttcg cctttgggcc
      421 ggagtcgcga tccgcgtggt agcggcggag gcgatagagc ggggtttcgt gcacgacggt
      481 gtacgccgac gattcctcac cggtgtcgag cccgccgaag cgcgccacct cgagcgcgtt
      541 tcgcgccatc gccgccagcc gctcggtcgt cgcagacagt gttcgagagg agagttccaa
      601 ggattcgaat cctgccataa cgaggcttgg attgatacgg gaaacgcgtc ggcgctgggc
      661 ggctcaacgg cggtcgttca ccgtctcgcc acgagcgcgg cgaagcgtgc cacgacgagg
      721 tcgcgcaccg gggccaacgc ctcgacggcc gggccacagc cttcacgcac cccgtgccga
      781 ccgccgagcg cagcgtcggc ctcgtcgggg aacgagtcga tgaatcgggt cgcctcgtcc
      841 acgccgacct cgaggtggaa ctgcagaccc caggtacaac ggccgacccg aaaggcctgc
      901 tgttcatacg cttgcgatcg ggcaaggtgg atcgcccccg cgggcagcga gtaggtctcg
      961 ccgtgccagt gcagcacatc gagatgcccg tcgagaccga agaagaggtc gtcgtcgacg
     1021 gcgcattcaa gcacctcgac ggcgccccag ccgacctcga ggccatggcc ggcgatcact
     1081 cgggctcccg cagcggcggc cagcagttgg gcgcccaaac acacgccgag ggtcggcacc
     1141 tcacgatcga gcgcctggcg cagcaacgcg atctcggcgc cgcgggtcgg gaatccgtcg
     1201 tcggaacgag ccgacatcgg accacccatc acgacgagcc cgtcgatgcc atcgaggtcc
     1261 tcagggatgc gttggccgag atcggcccga atcacgtcga cctcgacacc ggccgcgtcg
     1321 agtgcggtgg cgatgtgcgc ggggccctcc acgtcgagat gttggatgac cacggcgcgc
     1381 atggaacgtc agcttacggc ccgcctcgcg aagcgctgga acacgggatg ttcgtggcgc
     1441 cgcagatcga tcaccgggcc ggaggccgcg gccgggctgg gctcggagac cggttcaagt
     1501 tgctctgcgt tgtggtcgag ccacaccttc atcgactctg ccccagcctc gaccagccgg
     1561 gaacaggcgc gccagtcgct ggtgttgagc gacggatcga tcgtcggccg gacccgccag
     1621 agctgacgat cgatcgacgc tgcgtcgacg atggcgtcgg tcgccgcgtt cagggcgatc
     1681 gagaagctgc gggtcagcat gcccagcgcg gttcgaggaa ccgagtcgtt gcccgccgcg
     1741 gagacgtcga ggcacaggat cgaatcgcct ccgagctcag ccgcgcgttg caccggaagg
     1801 tttgccacca ctcctccgtc gacgaaactc attccgtcga tgaccgccgg cggaacgatc
     1861 gccgggagtg cggccgacgc atacagcacg tcgcgggcaa agcccgcatc aaaccagtgc
     1921 ggtagtccgg tgaggatgtt cgtcgccgac acgtgcagcg ggatcctcag gtcctcaagc
     1981 cgttcgatcg ggcacaggtc cgagatgagc gatcggagct tcgccccgtc ctgcacggag
     2041 gggcgccgcc gcaccaggtt gagcgcctgc gacagcacgc cctgggggaa cactccacgg
     2101 gaatcgaagt gcgaccagat gtcgctcagt gccttggtcg cgtcagcggt gaagtcgtgg
     2161 gcaacccaac tgccgttgag cgctccggcc gaggtcccca cgatgacgtc gggtctccag
     2221 ttcgaatgct ccgcaagcga ctgcagcatt cccgcttggg cggctccgaa gatcccgccg
     2281 ccgctgagca cgagcacgcg cttggccgac ggctccggcg ccgcctccgg tgcgtccgac
     2341 cctgcctcgg gttctcgggt gaggtgcggt cgcccccacc ccgaagaggt gaaggctcca
     2401 agattcagcg agcacttcat ggggggacgt tgagcccatg ggctacgaaa tgcaaccctg
     2461 cctttcggct caaacccgcg attcatgagg tcgatcgagc gaccgcgttt cacaccgcac
     2521 ccccccgggt acccaaacca gtgcagcgga gacagtcaca gcgctcatga ccgtccaaag
     2581 ccaccagcca gatctgttgg attgatccag cggagcccgc caaaccggac aaagtcgctg
     2641 tcgaacgagc agatgctggt cccgtgctcg atggcgagtg cggcgagatg tgcatcggtc
     2701 acgaggttcg ctcgcagatc gccgtcgatc agcaaacgct tgaggatgtc gcgatggcgc
     2761 tgaccagcag cgggcaccca cacctgcgcg atgtcgagcc agtccgagac gaacccccac
     2821 gcatccctgg ggctcaacgg gttcgcactc acacggggat gagtcatgat ccgctgaaac
     2881 gccatcaacg acacccatgg caacccgaca cgctcgttgc cgttcaacgc gtcttcgagc
     2941 cacgagcgag caacctcgtg gaaatgtgac tgttcgtcaa cggcgtagat caggacattg
     3001 gcgtcgacga tcaccgctcg gacccgtcgg cgacgacttc atcaaggagt tcgagagcgt
     3061 cggcgatgtc ggtgacgtcg atgcgcaacc ccacgctggc ggtacgttgc tggaatttgg
     3121 tgggcggcag ctctcgcgcc aatcccgcac gggcgagttc gttgacggcc tcaccaaggc
     3181 cgatgttgcg ctcgcgacgc agctgctcgg ccgccgccac cacctctgga tcaagccgca
     3241 ccgtagtcct catgggtt
//
LOCUS       DAOOMA010000281         3254 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_35862_length_3254_cov_3.719912, whole genome shotgun sequence.
ACCESSION   DAOOMA010000281 DAOOMA010000000
VERSION     DAOOMA010000281.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 3254)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3254)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3254
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35862_length_3254_cov_3.719912"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            110..1219
                     /gene="pstS"
                     /locus_tag="PLP95_08225"
     CDS             110..1219
                     /gene="pstS"
                     /locus_tag="PLP95_08225"
                     /inference="COORDINATES: protein motif:HMM:TIGR00975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0042301 - phosphate ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HPB45823.1"
                     /translation="MRIPDRFLRLVASVMLISITATACGLTDTSGDERFVSELDGLDI
                     DYRTLTGTLNGSGSSFQDPLQQAVSGEFRRVARNVTVNYTKSGSSAGKADLAARTVQF
                     AGTDSAIKAEERADFRDEVLYFPVVAAPITVSYNLAEVPELRLGPDTIARIFQGDIAV
                     WNDRRIAEENPGVELPDLRIAVVRRSDGSGTTKNFSSYLDDAAANVWRLGTGESLEWP
                     AATQGAEKSSGMSSLIASTSGAIGYVDLSDSVAARLQRALVRNRAGNYVPPKLPNARA
                     AIESSTFDDEFLYDPLNAPGDEAYPITAPTWVIVAARQRSESTADALRGYLNYLLTEG
                     QLIAPTLGYAAVPESIRQALIERIDLIRSSSEDRP"
     gene            1216..2193
                     /gene="pstC"
                     /locus_tag="PLP95_08230"
     CDS             1216..2193
                     /gene="pstC"
                     /locus_tag="PLP95_08230"
                     /inference="COORDINATES: protein motif:HMM:TIGR02138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HPB45824.1"
                     /translation="MTDLAIRPIDHTDLVDDRNDHRPDKLFSAAAMAAGYLVLVILAA
                     IAISTTVKSWPAFQAEGFGYFFRDEWAPSRGRFGAASIVYGTLVVSAIALVVAVPLSI
                     GIALFITEVAPRKVRSWITGIMDVLAAVPSVVFGLWGFLMLRPYLKDFYNAVADAVAG
                     VPVLRSIFGPSLSGQSFMTAGLIVGIMIVPIITSIVREVLSTVPINEKNGALALGATR
                     WEMIRGVVFPHSTGGMVGAVMLGLGRAMGETIAVALVIGASPQITANLFSQGDAMPSI
                     IARNLNEASGTYRAALIGLGVGLFLLTILINISARLLVRRVDARTKGAR"
     gene            2193..3191
                     /gene="pstA"
                     /locus_tag="PLP95_08235"
     CDS             2193..3191
                     /gene="pstA"
                     /locus_tag="PLP95_08235"
                     /inference="COORDINATES: protein motif:HMM:TIGR00974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HPB45825.1"
                     /translation="MPTTAPVVPFPSPESGESSGPNPLASTLSPRRRWSERLATSWMI
                     ASLVVASIPLLLVLGVLIVKGSSMVATVDWWTKDIPSNIASGALASQEAQEAFGNTGT
                     VREVEVVYGMAPAIVGTLITTGVAALLAIPLGILAAVYLNEYGGKSRSASIIRFFTDV
                     MTGVPSVVMGIFVYTVWVLTFEERGRSGLAGGLALACLMLPVVVRSTEEMLRLVPDSL
                     RQASAALGAPKWKTTLGVVVPAATPGITSGSMLAIARAAGETAPILFTVGVVTATNFS
                     LFGQNTTLSAQIFGNATQPGGEAMAWGAALTLILIVMVFTLAARWISGRFAVRIDG"
BASE COUNT          499 a         1108 c         1026 g          621 t
ORIGIN      
        1 ccgagtgttc ccgtcccgtt cacctgatgt tcacctccga ggtttcctgt cgcatgccgc
       61 gcttcgtaga ttcgccacgt acccccgccc tccaccagga gctggaccgt tgcgaatccc
      121 cgaccgcttc ctgcgactcg tcgcgagtgt catgttgatt tcgatcaccg ccacggcctg
      181 tggcctcacc gacacctccg gcgatgagcg gttcgtgtcc gaactcgacg gcctcgacat
      241 cgactatcgg acgctgaccg gaacgctgaa cggttcaggt tcctcgttcc aggatccgct
      301 ccagcaggcg gtgagcggcg agttccgtcg ggtcgcccgc aacgtgacgg tcaattacac
      361 caagtcggga tcctcggcgg gcaaggccga cctggcggct cgcaccgttc agttcgccgg
      421 aaccgacagt gcgatcaagg ccgaggaacg ggcggatttt cgcgacgagg tgttgtattt
      481 cccggtcgtc gccgcgccga tcacggtttc ctacaacctc gctgaggtgc cggagcttcg
      541 actcggaccc gacacgatcg cccggatctt ccagggcgac atcgccgtct ggaacgaccg
      601 ccggatcgcc gaggagaatc ccggcgtcga gttacccgac ctgcgtatcg ccgtggttcg
      661 acgttcggac ggctcgggca cgacgaagaa cttctcctcg tatctcgacg acgccgctgc
      721 gaacgtgtgg cgcctcggca ccggagaatc gttggagtgg ccggcggcga cccagggtgc
      781 ggaaaagtcg agcggaatga gttcgctcat cgctagcacg tccggggcga tcggctacgt
      841 cgacctctcc gactcggtcg ccgcacggct ccagcgggca ctcgttcgca accgggccgg
      901 caactatgtg ccgccgaagc ttccgaatgc ccgggccgcc atcgagtcgt ccacctttga
      961 cgacgagttt ctctacgacc cgctgaacgc tcccggcgac gaggcctatc cgatcaccgc
     1021 accgacctgg gtgatcgtcg ccgcccgtca gcgcagcgag tcgacggccg acgcgctgcg
     1081 cggctacctc aattacctcc tgaccgaagg gcaacttatc gcacccaccc tcgggtacgc
     1141 ggcggtgccc gaatccatcc gccaggcgct catcgagcgc atcgacctca tccgctccag
     1201 ctcagaggac cgtccgtgac cgatctcgcg atcagaccga tcgaccacac cgacctggtc
     1261 gacgaccgca acgaccaccg acccgacaaa ctcttcagcg cggcagcgat ggcggcgggc
     1321 tacctggtgc tcgtcatcct tgcggcgatc gccatctcga cgaccgtgaa gtcgtggccg
     1381 gcatttcagg ccgaggggtt cggctacttc ttccgcgatg aatgggcgcc gagccgcgga
     1441 cgattcggcg ccgcatcgat cgtctacgga accctcgtgg tgtccgccat cgcgctcgtc
     1501 gtcgcggtgc cgctgtcgat cggtatcgcg ttgttcatca ccgaagtggc tccacgcaag
     1561 gtccgttcat ggatcaccgg gatcatggac gtgttggccg ccgtaccctc ggtcgtgttc
     1621 gggctgtggg gtttcctgat gctgcggccc tacctcaagg atttctacaa cgcggtggcc
     1681 gacgcggttg cgggcgtgcc cgtcctgagg tcgatcttcg gcccgagcct gtccggtcag
     1741 agcttcatga ccgccggtct catcgtcgga atcatgattg tgccgatcat cacctcgatc
     1801 gtgcgtgagg tgctgagcac cgtgccgatc aacgagaaga acggggcgct cgcgctcggg
     1861 gccacacgtt gggagatgat tcgcggggtc gtgtttcccc actccaccgg cggcatggtc
     1921 ggcgccgtca tgttgggcct cggccgggcg atgggcgaaa cgatcgcggt agcgctcgtc
     1981 atcggcgcca gccctcagat caccgccaac ctgttcagcc agggcgacgc gatgccctcg
     2041 atcatcgccc gcaacctgaa cgaggcatcg ggtacctacc gcgccgccct catcggactc
     2101 ggcgtcgggt tgttcctcct cacgatcctc atcaacatct ccgctcgtct cctggtgcgc
     2161 cgggtcgacg ctcgcacgaa gggtgcacgc tgatgcccac caccgcaccg gtcgttccgt
     2221 ttccgtctcc cgaatcgggc gagtcgtccg gacccaaccc gctcgccagc accttgtcgc
     2281 cccgccgtcg ctggtcggag cgcctggcca cgtcgtggat gatcgcctcc cttgtcgtgg
     2341 cgtcgatccc gctgctgttg gtgctcggcg tgctgatcgt gaaagggtcc tcgatggtcg
     2401 ccaccgtcga ctggtggacc aaggacatcc cgtcgaacat cgcgtcgggc gcgctggcat
     2461 cgcaggaggc tcaggaagcc ttcggtaaca ccggaacggt tcgcgaggtc gaggtcgtgt
     2521 acggaatggc cccagccatc gtcggaacgc tgatcacgac cggcgtggcc gcgctgttgg
     2581 cgattcccct ggggattctc gccgcggtgt acctcaacga gtacggcggc aagagccgtt
     2641 cggcctcgat catccgcttc ttcaccgatg tgatgacggg tgtgccatcg gtcgtgatgg
     2701 gcatcttcgt ctacacggtg tgggtgctga ccttcgagga gcggggccgt tctgggctcg
     2761 cgggtggttt ggcgctggcc tgtctcatgt tgccggtggt cgttcgttcg accgaggaga
     2821 tgctgcgcct cgtccccgac tcgttgcgac aggcctccgc tgcgctcggc gccccgaagt
     2881 ggaaaaccac gctcggtgtc gtcgtgccgg cggcgacgcc tggaatcacc tcgggatcga
     2941 tgctggccat cgcccgtgcc gcgggtgaaa ccgccccgat cctgttcacg gtcggcgtgg
     3001 tgaccgccac caacttctcg ctgttcggcc agaacacgac cctgtcggcg cagatcttcg
     3061 gcaacgccac ccagccgggc ggcgaggcga tggcctgggg tgcggcgttg acgctcatcc
     3121 tgatcgtgat ggtgttcacg ctggcggcgc ggtggatctc aggacggttc gcggtacgaa
     3181 tcgacggctg agctcctcgg cacagcccca actcggcaca gccccaagac gttctgtagg
     3241 taattgcggt tgcc
//
LOCUS       DAOOMA010000282         3229 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_36223_length_3229_cov_3.988973, whole genome shotgun sequence.
ACCESSION   DAOOMA010000282 DAOOMA010000000
VERSION     DAOOMA010000282.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 3229)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3229)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3229
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36223_length_3229_cov_3.988973"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..401)
                     /locus_tag="PLP95_08240"
     CDS             complement(<1..401)
                     /locus_tag="PLP95_08240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020376849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lyase family protein"
                     /protein_id="HPB45826.1"
                     /translation="MSEQHRDAEPTTDDLAERAGEPNQHNAAAPLWHGRFAEGPAEEL
                     WEYTLSLPFDQRLAPYDIRGCVAHVAGLEAAGVLSATEAASVVEALAVTEAELANATF
                     EFVATDEDIHTAIERRVTELAGDAGARLHTGR"
     gene            474..1400
                     /locus_tag="PLP95_08245"
     CDS             474..1400
                     /locus_tag="PLP95_08245"
                     /inference="COORDINATES: protein motif:HMM:NF024118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HPB45827.1"
                     /translation="MRIVPFGTSSGRPTARRNVSGLGLDLGRRWVLVDCGDGTQQQIM
                     RSPLKIHRIDAVLLTHLHGDHVLGLPSLLGTMGMEGRTAPLTLVAPVGVRSWLDAMLN
                     LPILNLTFDVEVLELESAASLGTISGLEVTALPLRHRVPSFGYRFAEPERRGHVDAAA
                     ATALGLSGPDIGRLQRGERLDGFEGLDAAQIIGEARPGRVVTILGDTMYAKTSVELAL
                     DADLLVHECTYAASDHPYCGQWMHSCSLDVARVATEANVGHVVINHFSSRYPDESVLA
                     AEVRERVPDTTRVTAAVENDPIEIPFPDNQWS"
     gene            complement(1470..2666)
                     /locus_tag="PLP95_08250"
     CDS             complement(1470..2666)
                     /locus_tag="PLP95_08250"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015799054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HPB45828.1"
                     /translation="MAKKVVLAYSGGLDTSVAVRWMIENLGCEVICVSANIGQEGTLD
                     GNREKALGAGAIEYIELDLREEFARDYLAPIIKANALYEGKYPLVSALSRPLIVKHQV
                     EIARRFGADGVAHGCTGKGNDQVRFEVSTMALAPDLELVAPVRNWGFTREDSIAYAAE
                     HNIPIRATKEKMYSIDDNLWGRAIECGEMEDPWAKPPEGVWEMTRLTATENREITIAF
                     ERGVPVSVDGTAMPLFEIVEHLNSVVGSYGWGRLDMVENRRVGIKSRETYEAPGALAI
                     MLAHADLESITVERDLGREKMRLEHRYAELVYDGLWFSPLKQALDAFIEESQRFVTGE
                     VRLALEPNNCYVVGRRAPKSLYDYGLATYDAADRFRHSDSEGFVQLWGLGIKTWAGIQ
                     GAGTTD"
     gene            complement(2732..3211)
                     /gene="argR"
                     /locus_tag="PLP95_08255"
     CDS             complement(2732..3211)
                     /gene="argR"
                     /locus_tag="PLP95_08255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006525 - arginine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HPB45829.1"
                     /translation="MAKLTKLTKTARQHRIVKLLEDNLVTNQAQVVELLEADGYEVNQ
                     ATVSRDLEELAAVKVRLPGGDTAYAIPELPYERVTTDDHLKRVLGDWVAELDRSGDLV
                     VLRTPPGCAHVVASALDRSALADILGTVAGDDTILVIATESSGPEVEAEIARLAGLA"
BASE COUNT          497 a         1038 c         1108 g          586 t
ORIGIN      
        1 cgtccggtgt gcaggcgggc accggcgtcg ccggccagtt cggtgacgcg acgctcgatg
       61 gcggtgtgga tgtcctcatc ggtggcgacg aactcgaacg tcgcgttcgc cagttcggcc
      121 tcggtgaccg ccagcgcctc gacgaccgaa gccgcctcgg tggcactcag cacacctgcg
      181 gcctcgagtc cggccacgtg ggccacgcaa ccgcggatgt cgtagggggc gaggcgctga
      241 tcgaacggca acgacagcgt gtactcccac agttcctcgg ctggaccctc ggcgaatcgg
      301 ccatgccata acggagctgc agcgttgtgc tggttcggct cgcccgctcg ttcggcgagg
      361 tcgtcggtcg tcggttcggc gtcgcggtgc tgttcagaca tcgtcgccac gctagcgccc
      421 tgtctacgaa cggccggcga acggccggcg ggtcgcccgc gtaggctggc cggatgcgga
      481 tcgtcccctt cggcacttcc tcgggtcgcc cgaccgcgcg acgcaacgtg agcgggttgg
      541 gcctcgacct cggccgacgg tgggtgctgg tcgattgcgg ggacgggacc caacaacaga
      601 tcatgcgcag cccgctgaag atccaccgca tcgacgcggt gctgctcacc catcttcacg
      661 gcgaccacgt gttggggttg cccagcttgt tgggcacgat ggggatggag ggacgaaccg
      721 ctcccctgac ccttgtcgct ccggtcggcg ttcgcagttg gttggacgcc atgttgaatc
      781 tgccgatcct caatctcacc ttcgacgtcg aggtgctcga gttggaatcg gcggcctcgc
      841 tcggcacgat ttccggtctc gaggtcacgg ccctgccgct tcgccaccgg gtccccagtt
      901 tcggctaccg cttcgccgag ccggaacgcc gcggccatgt cgacgccgct gcggccaccg
      961 cactcgggct gtccggtccc gacatcggcc gactccagcg aggtgagcgc ctcgacgggt
     1021 tcgaagggct cgacgcggcc cagatcatcg gcgaggcgcg ccccggacgg gtcgtcacga
     1081 tcctgggcga cacgatgtat gcgaagacct cggtcgaact cgcactcgac gccgacctgc
     1141 tggtgcacga atgcacctac gccgcgtcgg atcaccccta ctgcgggcag tggatgcaca
     1201 gctgcagcct cgacgtggcc agggttgcga ccgaggcgaa cgtcggccac gtggtcatca
     1261 accacttctc gtcgcgctac cccgacgagt cggttctggc ggccgaggtt cgtgaacggg
     1321 tgcccgacac cacgcgggtc accgccgcgg tcgagaacga ccccatcgaa ataccgtttc
     1381 ccgataacca gtggtcgtga tcacgaccac tggttatcgg agaacgggcc gaagggggct
     1441 gaatcgggct gagttgggcc gaatcgggct cagtcggtcg tgccggcgcc ctgaatgccg
     1501 gcccaggtct tgatgccgag accccacagc tgcacgaatc cctccgaatc ggagtgacgg
     1561 aaccggtccg ccgcgtcgta ggtggccagc ccgtagtcgt agagcgactt gggggcgcgg
     1621 cgcccgacca cgtagcagtt gttgggttcg agcgcgaggc gcacctctcc ggtcacgaag
     1681 cgctgcgact cctcgatgaa cgcgtcgagg gcctgcttga gcgggctgaa ccacaagccg
     1741 tcgtagacca actcggcgta gcggtgctcc aggcgcatct tctcgcggcc gagatcgcgc
     1801 tcgacggtga tcgactcgag gtcggcgtgg gccaacatga tggcgagcgc gccgggggcc
     1861 tcataggtct cgcgggactt gatgcccacg cgacggttct cgaccatgtc gagccgaccc
     1921 cagccatagg agccgacgac ggagttcagg tgctcgacga tctcgaacag cggcatcgcc
     1981 gtgccgtcga ccgacaccgg aacgccacgt tcgaacgcga tggtgatctc gcggttctcg
     2041 gtcgcggtga gccgggtcat ctcccagacg ccctccggcg gcttggccca cgggtcttcc
     2101 atctcgccgc actcgatcgc tcgaccccac aggttgtcgt cgatggagta catcttttcc
     2161 ttggtggccc ggatcgggat gttgtgctcg gccgcatacg cgatggagtc ttcgcgggtg
     2221 aatccccagt tgcgcacggg ggcgacgagt tcgaggtcgg gggccaacgc catggtcgac
     2281 acctcgaaac gcacctggtc gttgcccttt ccggtgcatc cgtgggcgac accatcggct
     2341 ccgaaacgcc gggcgatctc gacctggtgc ttgacgatga gcggtcggct gagcgcgctg
     2401 accaacgggt acttgccttc gtagagcgcg ttggccttga tgatgggagc gaggtagtcg
     2461 cgggcgaatt cctcgcgcag gtcgagttcg atgtactcga tcgctccggc accgagggcc
     2521 ttttcgcgat tgccgtcgag cgtgccttcc tggccgatgt tggccgacac gcagatgacc
     2581 tcgcagccga ggttctcgat catccaacgc actgcgacgg aggtgtcgag acctccgcta
     2641 taggcgagca cgactttctt ggccatgggt ggtgttctct ttcgatgtcg ggggtggatt
     2701 tggtcgggct ggtgtccggc gggctggggg ctcaggcgag tccggcgaga cgggcgatct
     2761 ccgcctcgac ctccgggccg gacgactcgg tggcgatcac caagatggtg tcgtcgccgg
     2821 cgacggtgcc caggatgtca gcgagcgccg aacgatcgag cgcggaggca acaacgtgcg
     2881 cacaacccgg cggggttcgc aacacgacga ggtcgccgga tcgatcgagt tcggccaccc
     2941 agtcgccgag cacacgtttg aggtggtcgt cggtggtgac gcgctcatag ggcaactcgg
     3001 gaatggcgta tgcggtgtca ccgccgggga gccgaacttt caccgcggcg agttcctcga
     3061 ggtcgcggct gaccgtggcc tggttgacct cgtagccgtc ggcctcaagg agttcgacga
     3121 cctgcgcctg attggtgacg agattgtcct cgagcaactt gacgatgcgg tgttgtcggg
     3181 cggtcttggt gagcttggtg agtttggcca tctgcggatc tgcctgtgg
//
LOCUS       DAOOMA010000283         3228 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_36249_length_3228_cov_3.156319, whole genome shotgun sequence.
ACCESSION   DAOOMA010000283 DAOOMA010000000
VERSION     DAOOMA010000283.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 3228)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3228)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3228
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36249_length_3228_cov_3.156319"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1174
                     /locus_tag="PLP95_08260"
     CDS             <1..1174
                     /locus_tag="PLP95_08260"
                     /inference="COORDINATES: protein motif:HMM:NF013630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HPB45830.1"
                     /translation="IMGAGTVVLCSLGNARRSDGRLGAIDDDARNAAAVLESLAGSLP
                     PAADRAERAARRALRSTIQRIQRDGSAALLDASQRLTDAAQQSALSHTSSGRTSGQTP
                     TTANSTAGRRLSTPAAAVPSRIRALTLSVGTLAGFVAVILGLRWLGGPLQWPAADPLT
                     GAIDPITLVLTAGRVAGVLAAIYGIVLSAATAIAVLTGRAEVAAAAARLASPRVRTAM
                     LVLVGVGTVASAISGPIATPPTSSVASSAPARVAPVAAAEPTTAAPTTATPTTATPTT
                     AAPTTAAPTSQVDSTPAQAAPDADADNTAADNAGDVIDDDSRWTVARGDHLWGISAQI
                     VTDHLGGPATDADIGRYWSHLVDANRSNLVDPDNPDLLHVGQVLRVPSAADVFQTP"
     gene            1260..2549
                     /locus_tag="PLP95_08265"
     CDS             1260..2549
                     /locus_tag="PLP95_08265"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HPB45831.1"
                     /translation="MAIQTVVSGRIRNPALTGVRGMAAFVIFLHHAFGYVLEPRSSLD
                     TAIAGLAAPGFAALDWFFVLSGLFIGQILLTDGTSPEALKHYMSRRVLRIVPLYWLVL
                     AVVVIVFPLAVRAFGVGGEWKELADGYRTLWAPYATFANNIVLDAGSTAAEFSLSHFW
                     TVSLELQFYLLAPIVVILARRRVVHVAFGLALAIMAFRLGAAVLGTPHDAMYRQPWFH
                     SDSLFLGMCMGACQISGTSVPWSRIPRKLLIATVPLNLVIAMALASYRPDSGYATPFS
                     LTATSVAGVLVIENVLRPEPTRLHRALKRPFWMWSGDRSYPFYLLHVPVLVAVNLALG
                     EVLVIGAGDTSVRLPHTVLVAAVTFAVTMGLSHVLHVALERPLLRWASSRFRIQKRST
                     TSASSPLGRSPLEGQAEGIEPLCGLILSDPENRGDRP"
     gene            complement(2463..3020)
                     /locus_tag="PLP95_08270"
     CDS             complement(2463..3020)
                     /locus_tag="PLP95_08270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HPB45832.1"
                     /translation="MGTGQNVGYEDDEDDEVRLEFGREVGARLRAIRKQKRLSLQEVE
                     ASTGAEFKASVLGAYERGERAISVPRLFRLARYYGVPVDQLLPREDSTGYSAEDESRD
                     EATRSGQESRSTQLDLRRLEGIDSAEGETLRRYVSMIQVQRDDFNGRVLTIRRDDLRA
                     IAAILGVGQDQAAQRLDALGLTFEG"
BASE COUNT          504 a         1199 c          980 g          545 t
ORIGIN      
        1 catcatgggt gctggcaccg tcgtgttgtg ttcgctgggc aacgcccgac gcagcgacgg
       61 tcggctcgga gcgatcgatg acgacgcccg caatgcggca gcggttctgg agtcgctcgc
      121 ggggtcgctt ccccccgctg cggatcgagc ggagcgagca gcacggcgcg cccttcgttc
      181 gacgatccaa cggattcaac gcgacggctc cgccgcgctg ctcgacgcat cgcaacggct
      241 gaccgatgcc gcgcaacaga gcgccttgtc tcacacttcg agtggtcgaa catccggcca
      301 aactccgacg accgccaact cgacggccgg ccgccgcctc tccacgcccg cggcggccgt
      361 tccatcgcga atcagggcac tcacgctcag cgtcggcacc ctcgccgggt tcgtcgccgt
      421 gatcctcgga ctgcgatggc tgggcggccc gcttcagtgg cccgcggccg acccgctcac
      481 cggggcgatc gacccgatca cgctggtgct caccgctggc cgtgtcgccg gagtcctcgc
      541 cgcgatctac ggcatcgtgc tgagcgctgc gacggcgatc gcggtgctca ccggtcgcgc
      601 cgaggtcgcc gcggccgcgg cacggctcgc ctcaccaagg gtgcgcacgg ccatgctggt
      661 gctggtcggg gttgggacgg tcgcgagtgc catcagcggt ccgatcgcca caccacccac
      721 gtcgtcggtg gcgtcgagcg cgcccgctcg ggttgcgccg gtggccgccg ccgaaccgac
      781 caccgccgct cctaccaccg cgaccccaac gaccgcgacc ccaacgactg ccgcgcccac
      841 caccgccgca cccacctctc aggtcgattc cacgccggca caagcggctc cggacgccga
      901 cgccgacaac accgccgccg acaacgccgg cgacgtcatc gacgacgaca gccgctggac
      961 cgtggcccgc ggcgatcacc tctggggaat cagtgctcag atcgtgaccg atcacctcgg
     1021 cggcccggca accgacgccg acatcggccg ctactggagc cacctcgtcg acgccaaccg
     1081 ttccaacctc gtcgaccccg acaaccccga cctcctccac gtcggccaag tcctccgcgt
     1141 cccttcagcc gccgacgtgt tccagacacc ctgaaggcga ttgagtatcg acgggttcac
     1201 gccacccgct accctcaacg agaccaccac gggggacgtg ccggagcgac aggaaccaga
     1261 tggcgataca gacggttgtt tccgggcgca tacgcaaccc ggcgctcacc ggcgttcgag
     1321 gcatggccgc attcgtcatt ttcttgcacc atgcgttcgg atacgtgctt gagccacgtt
     1381 caagcctcga caccgcgatc gccgggctcg ccgcccccgg gttcgcagca ctcgactggt
     1441 tcttcgtgct gtcggggctc ttcattggcc agatcctcct caccgacggc acctcccccg
     1501 aggcactgaa gcactacatg tctcgccgag tgctccgaat cgttccgctg tactggctgg
     1561 tactcgccgt cgtcgtcatc gtgttcccgc tcgcggtgcg tgcgttcgga gtcggcggcg
     1621 agtggaagga gctcgcggac gggtaccgaa cgttgtgggc cccctacgcc acctttgcaa
     1681 acaacatcgt gttggacgcc ggctccaccg ccgcggagtt ctcactgtcg catttctgga
     1741 cggtctcgct ggaactgcag ttctacctcc tcgccccgat cgtcgtgatt ctcgctcgcc
     1801 ggcgagttgt tcacgtcgcc ttcgggttgg cgcttgcgat catggcattt cgcctggggg
     1861 ccgcggtgct cggcactccc cacgacgcga tgtatcgcca accgtggttc cattccgact
     1921 ccctgttcct ggggatgtgc atgggcgcgt gccagatctc cggcacgagc gtgccgtgga
     1981 gccgaatccc acgaaagctg ctcatcgcaa cggtcccgct caacctcgtc atcgcaatgg
     2041 cactcgcctc gtaccggccg gactccggct acgcaacgcc gttcagcctg accgcaacga
     2101 gtgtggccgg ggtcctggtc atcgagaacg tgcttcgtcc cgaaccaact cggctgcacc
     2161 gtgcgttgaa acgtccattc tggatgtggt cgggcgaccg cagctacccg ttctacctcc
     2221 tgcacgtacc ggtgttggtc gccgtcaacc tcgcgctcgg cgaggtcctc gtgatcggcg
     2281 ccggcgatac ctcggtgagg ctgccacaca cggtgctcgt cgcagcggtc acgttcgcgg
     2341 tgacgatggg gttgtcacac gtcctccacg tcgcgttgga acgtccactg ctgagatggg
     2401 catcgtcgcg gttccggatt cagaaacgat cgaccaccag cgccagctcc ccgctgggcc
     2461 gctcacccct cgaaggtcag gccgagggca tcgagccgct gtgcggcctg atcctgtccg
     2521 accccgagaa tcgcggcgat cgcccgtaga tcgtcacggc gaatcgtcaa gaccctgccg
     2581 ttgaagtcgt cgcgctgcac ctggatcatg ctcacatatc gccgcagcgt ctccccctcg
     2641 gccgagtcga tcccctcgag acggcgcagg tcgagctgtg tcgaacgaga ttcctgaccg
     2701 cttcgagtgg cctcgtcacg agactcgtcc tcggcgctgt agccggtcga gtcctcgcgg
     2761 ggaagcagct gatcgaccgg taccccgtag tagcgggcga gtcgaaacag gcgcggcacc
     2821 gagatcgcac gctcgccgcg ttcgtacgca ccgagcaccg atgccttgaa ctccgcaccg
     2881 gtcgacgcct cgacctcctg cagcgagagg cgcttctgct tgcggatcgc acgcaagcgc
     2941 gcgccgacct cgcggccgaa ctccaagcgg acctcgtcgt cctcgtcgtc ctcgtagccc
     3001 acgttctgcc ccgttcccac gaggcgctgg acctcgcatc acctttgcga catcgacgtc
     3061 accggcgaca ccctggatcg ccggcgatca ccgtcaagac gttcgaaacc cggaaactta
     3121 gggcatcgcg cacgctgcga caacacgcag cgtggccgaa ccctcgctca gagcgccgag
     3181 gagcgcagcg ccgccaacag ggcgccggcg gtgatcgccg ccgtctcg
//
LOCUS       DAOOMA010000284         3225 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_36283_length_3225_cov_4.206940, whole genome shotgun sequence.
ACCESSION   DAOOMA010000284 DAOOMA010000000
VERSION     DAOOMA010000284.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 3225)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3225)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3225
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36283_length_3225_cov_4.206940"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..649
                     /locus_tag="PLP95_08275"
     CDS             <1..649
                     /locus_tag="PLP95_08275"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HPB45833.1"
                     /translation="PDVAYITVAPSAHQLTNPYAVTQCLGQLGELGYRAAVTSALRGS
                     DAHVFEAAGFVRRERLHVLRHELGNLPPIDRTGARLLRRGRRRDRRVVLDVDERAFER
                     FWALGSDGLDEALNATASVRFRVASDRDVFGYAVTGRSGEVGYLQRLAVDPVRSGGGI
                     GTALVLDALHWLRDRHGRFMLVNTQESNAAALRLYLRLGFNMVDEHLTVLQWPGE"
     gene            721..2709
                     /locus_tag="PLP95_08280"
     CDS             721..2709
                     /locus_tag="PLP95_08280"
                     /inference="COORDINATES: protein motif:HMM:NF039636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6049 family protein"
                     /protein_id="HPB45834.1"
                     /translation="MGAQPIPEGEGTEPSTSAIQLESVTPWVTESGTFRAVFDADGFP
                     EGAKLTYRIRQPVSGTESDRREQLIDLWNGTARTNVLHANTTVDVADLIQGDRVLLDI
                     PIRARSTRRSGAVLIPGPGTYAVDVTIRSGSSVIYETQLSLNALPAPERQSTPFQLAV
                     VANLSGPIGSDESGTIDDTAAVERTLQRYRDLIEDAAGAPIAVSISPQLLVTAPSDAQ
                     PPRLMDALTSASTVERPWADLDLEAWAKIDAAQLVRTSLLDGAQALSALGVSATQRTM
                     WFDGATVGTQALPLLARLGYRIVSLDDGRITGFTAPTGESGYTRPVQLASSPPMSALV
                     TDPVVADLLSGDLRDSVLAAHRLLTLFSTAHLAGQEPRGASIVVGDDVDPDVVAALYR
                     DVAEGTDALEHPIDASADTNSTTTTTRQPTGDPPIRAAVELVLPDQLNTLSPLTSTDQ
                     GQPTVTPVDSPSVASVASVVPEARALIDDYATIIGSGTDDARRISNVVQRSLDRRIEP
                     SVQAGMLNDVIDEMNQALGAITVSKPRALNVTERNTTIPLRVVNDLDRPINVRLRLKS
                     SRMNFVDGDVQALSLEPGVNPIPIRVEVLTSGQFTMEAEVLAPESTRVIASTRQHIRS
                     TAFSGVGLVLSGGALIFLVVWWLRTPHRKRPADQPDER"
     gene            2773..>3225
                     /locus_tag="PLP95_08285"
     CDS             2773..>3225
                     /locus_tag="PLP95_08285"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HPB45835.1"
                     /translation="MNSESRGPVLASPGLLIGGRYELRANVGAGAMAQVWSATDQVLN
                     RVVAVKILHQHLLSDPASVERFRREAYAIAALAHPNIVGVYDTVFDGAIQAIVMEYVD
                     GVTLRQLLDERKTLAEPELVELLDGIAMALDHAHRNGIVHRDIKPANIL"
BASE COUNT          565 a         1166 c          957 g          537 t
ORIGIN      
        1 ccccgacgtt gcctacatca cggtggcgcc gtccgcgcac cagctgacca acccgtacgc
       61 cgtcacccaa tgcctggggc agctcggcga actggggtac cgggccgccg tgacctccgc
      121 gcttcgcggt tccgatgccc acgtgttcga ggcggccgga ttcgttcggc gcgaacggct
      181 gcacgtgctg cgccacgaac tcggcaacct cccgccgatc gaccgaacag gtgctcggct
      241 gctgcgtcgc ggacgtcgac gcgaccgtcg ggtcgtcctc gacgtcgacg aacgagcctt
      301 cgaacgtttc tgggctctgg gaagcgacgg gctcgacgag gcactcaacg cgacggcgtc
      361 ggtgcgcttt cgcgtcgcca gcgatcgcga cgtgttcggc tacgcggtca ccggacgctc
      421 cggcgaggtc ggctaccttc agcgactcgc cgtcgacccg gtccgcagcg gcggcggaat
      481 cggtacagcc ctcgtgctcg atgcgctgca ctggcttcgc gatcgtcacg gtcgattcat
      541 gctcgtgaac acccaggagt ccaacgccgc ggccttacgg ctgtacctcc gcctcgggtt
      601 caacatggtc gacgaacacc tgaccgtcct gcagtggccg ggcgaatgag gcgctcatct
      661 cgtgcgttat ggcgcgtggc ggcgatcatc gccgctgtca cctgcctgta cccctcaccc
      721 gtgggcgcgc agccgattcc agagggcgaa ggcaccgagc cgtcgacgtc ggccattcaa
      781 ctcgagtcgg tgacaccctg ggtgaccgaa agcggcacgt ttcgggccgt cttcgacgcc
      841 gatggtttcc ctgagggcgc caagctcacc tatcgcatcc gccaaccggt ttccggcacc
      901 gagtccgacc gtcgagagca gttgatcgac ctgtggaacg gcaccgcccg aacgaacgtg
      961 ttgcacgcca acaccaccgt ggacgttgcc gatctcatcc aaggcgaccg cgtgctgctc
     1021 gacatcccga ttcgcgcccg ctccactcgc cgctcgggcg cggtgttgat ccccgggccg
     1081 ggcacctatg cggtcgacgt cacgatccgg tccgggtctt cggtgatcta tgagacccaa
     1141 ctttcactca acgcactgcc ggccccagaa cgtcaaagca caccgtttca attggccgtc
     1201 gtggcgaacc tgtccgggcc gatcggctcc gatgagtccg gcacgatcga cgacacggcg
     1261 gcggtcgaac gaaccctgca gcgctatcga gatctcatcg aagacgccgc gggcgcgccg
     1321 atagcggtgt cgatctcgcc acaactcctg gtcaccgccc cgtccgatgc acagccgccg
     1381 cgcctcatgg acgccctcac cagtgcctcg accgtcgaac gcccgtgggc cgacctcgac
     1441 ctggaagcct gggcgaaaat cgacgctgcg caactcgtgc gaacctccct gctcgacgga
     1501 gcacaagcgt tgagtgcact cggcgtcagt gcgacacagc gaaccatgtg gtttgatggc
     1561 gcaaccgtcg gcacgcaggc gctcccgttg ttggcacggc tcggctaccg catcgtttcg
     1621 ctcgacgacg gacgcatcac gggcttcacc gctcccaccg gcgagtcggg atacacacga
     1681 ccggtacagc tcgcgtcgag tccgcccatg tccgccctcg tgaccgaccc cgtggtcgcc
     1741 gacctgttgt cgggcgacct ccgcgactcc gtcctcgccg cccatcgact tctcacgctg
     1801 ttcagcacag cgcatctggc gggccaagag ccccgcggcg cctcgatcgt cgtcggcgac
     1861 gatgtggatc cggacgtcgt cgccgcgctc taccgcgatg tcgctgaagg caccgacgcg
     1921 cttgagcacc cgatcgacgc gtcggccgac accaactcga cgaccaccac aacacgccaa
     1981 ccgacgggcg acccaccgat acgcgccgcc gtcgaactcg tccttcccga ccagttgaac
     2041 accctgtcgc cactcaccag caccgatcaa ggccaaccga cggtgacacc cgtcgactcg
     2101 ccgtcggtgg cgtcggtggc gtcggtcgtc cctgaagccc gggcgctcat cgacgactac
     2161 gccacgatca tcggctccgg caccgacgat gcccgccgaa tctccaacgt cgtgcaacgg
     2221 tcactcgacc gtcggatcga gccatcggtc caggccggga tgctcaacga cgtgatcgac
     2281 gagatgaacc aggcgctcgg cgccatcacg gtctccaagc cccgcgcgct caacgtgact
     2341 gagcgcaaca ccaccattcc attacgggtc gtcaacgacc tcgaccgccc catcaatgtc
     2401 cggctacgcc tgaagagttc gagaatgaac ttcgtcgacg gcgacgttca ggcgctgagc
     2461 ctggaacccg gggtcaatcc cattcccatc cgggtcgagg tgttgacttc cgggcagttc
     2521 acgatggagg cggaggtact cgcgcccgag tcgactcgtg tgatcgcttc gacacgccag
     2581 cacattcgct ctacggcatt cagtggggtc ggtctggtct tgtccggcgg agcactcatc
     2641 ttcctagtgg tgtggtggct acgaactccg caccgcaaaa ggccggccga tcaacccgac
     2701 gaacggtgaa cccgggaccg tcacccaccc ggcgccggac cgacactgaa gcgccccgat
     2761 agcctcacac gcgtgaactc ggagtcacga ggaccagttc tcgcctcacc cggcctcctc
     2821 atcggaggtc gctacgaact gcgggcaaac gtcggcgcgg gcgcaatggc ccaggtgtgg
     2881 agtgctaccg accaggtgct caaccgagtc gtcgcggtga agattcttca tcagcatctg
     2941 ctcagtgatc cggcttcggt cgagcggttc cgtcgggagg cgtacgcgat cgccgcgctg
     3001 gctcatccca acatcgtcgg tgtctacgac accgtcttcg acggcgccat ccaggcgatc
     3061 gtcatggaat acgtcgacgg ggtcaccctt cgacaactcc tcgatgaacg caagacgctc
     3121 gccgaacctg aactcgtcga actcctcgac ggcatcgcca tggcgctcga ccacgcccat
     3181 cgcaacggca tcgtgcaccg agatatcaag ccagcgaaca tcctc
//
LOCUS       DAOOMA010000285         3199 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_36696_length_3199_cov_3.797710, whole genome shotgun sequence.
ACCESSION   DAOOMA010000285 DAOOMA010000000
VERSION     DAOOMA010000285.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 3199)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3199)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3199
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36696_length_3199_cov_3.797710"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..714
                     /locus_tag="PLP95_08290"
     CDS             <1..714
                     /locus_tag="PLP95_08290"
                     /inference="COORDINATES: protein motif:HMM:NF013499.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold nucleic acid binding domain-containing
                     protein"
                     /protein_id="HPB45836.1"
                     /translation="RKEHDMGVMSLFGEMEEGPAFDERVRIPDVEFDKKHRLAFEKEM
                     LGLYVSDHPLLGAEQALKRKVDCTILELGDLDDGTQRTVGGVITGLQRKWTKKGDLMA
                     VFNLEDLQTSVECMVFPKSMADHGHKLVDDAVVLVTGRVDKRDDQPKLIPRDIELFEP
                     MVEGAPPLRIQVNPNQLSDDNIGRLHTLFQEFPGESEVFLHLGARQVLRLPDAFGVDI
                     DSGLVGELRVLLGAEAIIV"
     gene            821..1489
                     /locus_tag="PLP95_08295"
     CDS             821..1489
                     /locus_tag="PLP95_08295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB45837.1"
                     /translation="MAFDVSTKDCSQLTDEELAEMADLCAEAGANFDLEVIGSQRDSW
                     VLVTQIREGGHLRGYSFSTLERIGGTPAIIVGLAHIRRNAKRDQVVRSIMSENYRRAL
                     MAFPDEDVLVGTRLASVDGYDAFKVLNDINPRPDHKASGEERAWGRRLAKRFGVDANY
                     DDKAFVVTGSGTAAVVFDHESLKPEKHEAGYVEFFNGLKAANGDCLIAFGWILAEDLL
                     KYNG"
     gene            1493..2095
                     /locus_tag="PLP95_08300"
     CDS             1493..2095
                     /locus_tag="PLP95_08300"
                     /inference="COORDINATES: protein motif:HMM:NF013077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HPB45838.1"
                     /translation="MDLAEALAARRSCRSYRSDPIDDELLDRVLSASLRGPSAGNSAS
                     LEWLVLNGPDEVGRYWDVTLPVSRRDRFPWPGLLRAPVLAIPAVRPEAYLERYSESDK
                     RHSGLGDDVAAWTVPYWWVDGGAGVMAMLLAATAEGLGSLLFGQFRHEAAVAQKFGVP
                     AHYRLLGTVALGWPDGGDRSSRSARRGRRELSSVTHRGVW"
     gene            2158..>3199
                     /locus_tag="PLP95_08305"
     CDS             2158..>3199
                     /locus_tag="PLP95_08305"
                     /inference="COORDINATES: protein motif:HMM:NF015155.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HPB45839.1"
                     /translation="MMKRFWTVLATTAARRAGLVTVVGLLLTLTMGFGATRLTFATGT
                     DSYLNADEPSYLTSVDFADRFGGDANLSVITMDKGHTVAELLTDPHNDEQLAAATAEL
                     RDIDEMLAVVDLRTTLEFTNSMISSDPDNPTLATLLDSVGARALADATERSEGHDKEV
                     READTAATVARVTAIPAEQMRFGTPEWTDFLLYDNAGEVRASQRMVIPDSTHALIIAR
                     FAGNLDVDVATAATNKAYDIVAGLDLNNATVRTTGAPKLLAEINEYLRGGMLTLGAIA
                     LGIMAVILLVLFDVRWRLLPLAVVAIGVIWAFGTAGFLGIPLTLVTVAGLPVMLGIGI
                     DYAIQLHARVEEE"
BASE COUNT          532 a         1028 c         1090 g          549 t
ORIGIN      
        1 cgcaaggagc acgacatggg cgtcatgtcg ctcttcggcg agatggagga ggggcccgcg
       61 ttcgacgagc gggtccgcat ccccgacgtc gagttcgaca agaagcaccg gctcgcgttc
      121 gagaaggaga tgctcggcct ctacgtgtcg gaccatccgc tgctcggcgc cgagcaggcc
      181 ctcaagcgca aggtcgactg cacgatcctc gagctcggcg acctggacga cggcacccag
      241 cgcacggtcg gcggggtcat caccggcctg cagcgcaagt ggaccaaaaa gggcgacctg
      301 atggccgtgt tcaacctcga ggatctccag acctcggtgg aatgcatggt gtttcccaag
      361 tcgatggccg atcacgggca caaactcgtc gacgacgcgg tggtgttggt gaccggtcgg
      421 gtcgacaagc gagacgatca gcccaagctc atcccgcgcg acatcgagtt gttcgaaccg
      481 atggtggagg gcgcgccgcc gcttcggatc caggtgaatc ccaatcagct gtccgacgac
      541 aacatcgggc gcctccacac gctgtttcag gagtttccgg gcgagtccga ggtcttcttg
      601 cacctcggcg cccgccaggt gctgcgcctg cccgacgcgt tcggcgtcga catcgactcc
      661 gggctggtgg gcgagttgcg ggtgctgctc ggcgccgagg cgatcatcgt gtgagacgcg
      721 ggtcgggccc cgcggcatgg ggcccggttt gacccaagta agatagggcg ctgaactggt
      781 gacaccggtt tttgacgttc cgattgaggt gaggggttcc atggcgttcg acgtcagcac
      841 gaaagactgc agccagctga ccgacgagga gctggcggaa atggcagatc tgtgtgccga
      901 ggccggcgcc aacttcgatc tcgaggtgat cgggagccag cgtgactcgt gggtgctcgt
      961 gacccagatt cgtgaaggtg gccaccttcg cggctactcg ttttccacgc tcgagcgcat
     1021 cggcggcacg ccggccatca tcgtcgggct cgcgcacatc cgtcgcaacg ccaaacgcga
     1081 tcaggtcgtg cggtcgatca tgagcgaaaa ctaccggcga gcgctgatgg cgttccccga
     1141 cgaggacgtt ctcgtcggta cccggttggc ttcggtcgac ggctacgacg cgttcaaggt
     1201 gctcaacgac atcaaccccc gccccgacca caaggccagc ggtgaggagc gtgcgtgggg
     1261 gcgacgtctc gccaagcggt ttggggtcga cgcgaactac gacgacaagg ccttcgtcgt
     1321 gacgggttcg ggcaccgcgg cggtcgtgtt cgaccacgag tcgctcaagc ccgaaaagca
     1381 cgaggcgggc tatgtcgagt tcttcaacgg actcaaggcc gcgaacggcg actgcctgat
     1441 cgccttcggc tggatcctcg ccgaggacct gctcaagtac aacggctgat ttatggacct
     1501 tgccgaggcg ctggccgctc ggcgttcctg tcggtcctat cgcagcgacc ccatcgacga
     1561 cgaactcctc gatcgggttc tgagcgcctc gctgcgcggc ccgagcgcgg gcaattcggc
     1621 gtcgttggag tggctggtgt tgaacgggcc cgacgaggtc ggccgatact gggacgtgac
     1681 cttgccggtc tcacggcgtg atcgcttccc gtggcccggg ttgcttcggg caccggtgct
     1741 ggcgatcccc gcggtgcggc ctgaggccta cctcgagcgc tacagcgaat ccgacaagcg
     1801 acacagcggc ctcggcgacg acgtcgccgc atggacggtg ccgtactggt gggtggacgg
     1861 cggagccggg gtgatggcga tgctgctggc cgcgaccgcc gagggtctcg gatcgctgct
     1921 gttcggtcag ttccgccacg aggcggcagt ggcgcagaaa ttcggggtgc cggcgcacta
     1981 ccgactgctg ggcacggtgg cgctcgggtg gcccgacggc ggcgatcggt cgtcgcgatc
     2041 ggcccggcgt ggacgtcgcg agctttcgag tgtcacacac cgcggcgttt ggtgaagaat
     2101 gggaccgatg cgtcgccgtc ggtgccgcat cgccgaccac cgaaactggg agtagcgatg
     2161 atgaagcgtt tttggacggt gctcgccacg acggccgcac gccgcgccgg cctcgtgacc
     2221 gtggtgggat tgctgctgac gttgaccatg gggttcggcg cgacgcggct gaccttcgcg
     2281 accggtaccg acagctacct gaacgcggat gagccctcgt atctcaccag cgtggatttc
     2341 gccgaccgct tcggtggcga cgccaacctg agcgtcatca cgatggataa gggccacacc
     2401 gtcgccgagt tgttgaccga cccccacaac gacgaacagc tcgccgcagc gacggccgag
     2461 ttgcgcgaca tcgacgagat gctggcggtg gtcgaccttc gcaccaccct cgagttcacg
     2521 aattcgatga tctcctccga ccccgacaac ccgacgctcg ccacgctgct cgactcggtg
     2581 ggcgcccgcg cgctcgcgga cgcgaccgaa cgttccgagg gccacgacaa ggaggttcgc
     2641 gaggccgaca ccgcggccac cgtcgcacga gtcacggcga tccccgccga acagatgcgc
     2701 ttcggcaccc cggaatggac cgacttcctg ctctacgaca acgccggaga agttcgggca
     2761 tcgcagcgga tggtcattcc ggactccacc cacgcgctca tcatcgcccg cttcgccggc
     2821 aacctcgatg tcgatgtagc gacggcggcg accaacaagg cctacgacat cgtcgccggt
     2881 ctcgacctga acaacgcgac ggttcgcacg accggagccc cgaagctgtt ggccgagatc
     2941 aacgagtatc tccggggcgg gatgttgacc ctcggcgcca tcgcgctcgg catcatggcg
     3001 gtgatcctgt tggtgctgtt cgacgtgcgt tggcgcctgt tgcccctcgc cgtggtggcc
     3061 atcggggtga tctgggcgtt cggcaccgcc gggttcctcg ggatcccgct gacgctggtg
     3121 accgttgcgg ggctcccggt gatgttgggg atcggtatcg actacgcgat ccaactccat
     3181 gcccgcgtcg aggaggagg
//
LOCUS       DAOOMA010000286         3192 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_36810_length_3192_cov_4.318776, whole genome shotgun sequence.
ACCESSION   DAOOMA010000286 DAOOMA010000000
VERSION     DAOOMA010000286.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 3192)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3192)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3192
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36810_length_3192_cov_4.318776"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            112..1056
                     /locus_tag="PLP95_08310"
                     /pseudo
     CDS             112..1056
                     /locus_tag="PLP95_08310"
                     /inference="COORDINATES: protein motif:HMM:NF020337.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CotH kinase family protein"
     gene            complement(1165..1878)
                     /locus_tag="PLP95_08315"
     CDS             complement(1165..1878)
                     /locus_tag="PLP95_08315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HPB45840.1"
                     /translation="MTTTATGDRLVILVVEDEESYVDALELGLRREGFEVEVARTGSE
                     ALARFAEVAPDLVLLDVMLPGISGIDVCREIRATSQVPIIMVTAKAAEIDTVVGLEVG
                     ADDYVTKPYRLRELVARIRAVLRRRVDATPAESHDEIVAVGDLVINVDSREVRRNGEL
                     LALPLKEFELLLLLMENSPRVLTRGTIIDRVWGYDYVGDTKTLDVHVKRLRAKIEDNP
                     AAPQRLLTVRGVGYKYNSV"
     gene            complement(1893..3140)
                     /locus_tag="PLP95_08320"
     CDS             complement(1893..3140)
                     /locus_tag="PLP95_08320"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPB45841.1"
                     /translation="MVIVLIVSVVVLVAAVVALIAIVLGQRSELSALRANVVEPGVDD
                     VDTSTELARLVAALEAVELGVVVVDPSGEVAFENGVASRLMTSSDSRAIVRQATIELI
                     SEAATGRSGRREVDLFGPPKATYLVVASPLVVSSEAPQQGVVVVIEDISERRRTEQVR
                     RDFVTNISHELKTPVGALGLLAETVDAETDPATVARFTKRIVEEVHRLAATIDDLLEL
                     SRIEFADDLELTEQVLGPIVAEAAARLDSAAAAREIELKVIGDPDRSVFADQRQLTSA
                     LSNLIENAIKYGADASVVTIDMGPPTGGRDGAGGGRDDADGHGADGDVTVISVSDRGD
                     GIPTRDLDRIFERFYRVDRARSRVTGGTGLGLAIVRHIVNNHGGSVTVTSKEGQGSTF
                     TVKIPARNPALSATPRPTESRPT"
BASE COUNT          569 a         1060 c         1008 g          555 t
ORIGIN      
        1 gggtcggacg tcgctgcgtt ctcgggggcc gtcgagatcc gcggccgcgg taacaccacc
       61 tgggacctgc cgaagaagcc gtaacggctc aagctcgcca acaaggccgc gctcatgggt
      121 atgccctcga accgtcactg ggccctcctg gcgaactacg ccgaccgttc ccagatccgc
      181 accaccgtcg gccagacgct cgcctcgatg acctccatcg actggtcccc gcgttcgcag
      241 ctggtggaag tcgtgctcaa cggggtgtac cagggcccgt atcagctcat cgaacaccga
      301 cgcatcgaca aggaccgcat caacatcgat gaaatgtcgt cgagcgacaa cagcggcgaa
      361 gccctcaccg gtggatacgt cttcgagatc gatttccgcg gcgacgacca ggccctgcgc
      421 accagtcgcg gcgcaaaggt cacggtgagc gatcccgagc cgtacacccc cgaacagcag
      481 gcctacgccc agtcggtgct gcaacgcttc gaagacgctc tgttctcgcc ggacttcgcc
      541 gacccggaaa ccggatatcg cgcgtacgtc gatatggagt ccctcatcga ttcgtacctg
      601 gtggcggaat tcacgatgca ggtcgacttc ttctacacga gcaccttctt ctacaaaaag
      661 cgcggcgacg agaagttcta cttcggcccg atgtgggatt tcgacgtttc ggtcgctccg
      721 gtcaccgtcg gggtcgagga gatcacgtgg ccagcgaaca tgccgtgggt ccgcaatccc
      781 tccatcacct tcaaccgcga cggcggcggc aagtggatcg ggcgactctt cgaggacccg
      841 acattcgtcc aagccgtcca tgaccgctgg caggagctca aggaaccgtt cggcgcctat
      901 gtccagggaa tggccgccat gcaggctccg ctcaacagcg ccatcaaggc cgacagcgtc
      961 cgttgggacc gtggcgagtt gggtacgtat caccgggcca gccagatgca gggctggatg
     1021 aaccagcgct ggaactggat gaattcaacg atgtagtgaa ctcgatgatg taacggccgc
     1081 gacgcggtga tggccgtcgg gcgggatcca ctgaggatcc cgcccgacgg ctttttcgtg
     1141 ggacccgagc cccatcgcgt tggctcagac cgagttgtac ttgtaaccga cgccgcgcac
     1201 cgtcaacagt cgttgcggcg ccgccggatt gtcctcgatc ttggcccgca gtcgcttcac
     1261 gtgaacgtcg agggtcttgg tatctccgac atagtcgtag ccccacacgc gatcgatgat
     1321 ggtgccgcgg gtcaacaccc gcggtgagtt ctccatcagt aacaacaaca gctcgaattc
     1381 cttgagcggc agcgccagca actcaccgtt gcggcgcacc tcccggctgt cgacgttgat
     1441 caccaggtcg ccgacggcga cgatctcgtc gtgggattcg gctggggtgg catcgacccg
     1501 acggcgaagg acggcccgga tccgggcgac cagctcgcgc aaccggtacg gcttggtgac
     1561 gtagtcgtca gcccccacct cgaggcctac cacggtgtcg atctctgcgg ccttggcggt
     1621 caccatgatg atcggcacct gtgacgttgc gcggatctct cggcacacgt cgatcccgga
     1681 gatccccggc aacatgacgt cgagcaacac caggtcgggc gcgacttcgg caaatcgtgc
     1741 aagcgcctcg gacccggtcc gggccacctc gacctcaaaa ccctcgcggc gcagccccaa
     1801 ctcgagtgcg tcgacatagg actcctcatc ctcaacgacc aggatgacga gtcggtcgcc
     1861 ggtggcggta gtggtcacgt tgtgttctcc ggtcatgtcg gtcggctttc ggtcggccgc
     1921 ggcgtcgccg atagggctgg gttccgggca ggaatcttca cggtgaacgt cgaaccctgc
     1981 ccctccttcg aagtgacggt caccgacccg ccgtggttgt tcacgatgtg gcgcacgatc
     2041 gccagcccaa ggccggttcc gccggtgacg cggcttcgcg cacggtcgac ccggtagaac
     2101 cgttcgaaga ttcgatcgag gtcgcgggtg ggaatgccgt cgccgcggtc gctcacactg
     2161 atcaccgtga cgtcgccatc agcgccgtgt ccatccgcgt cgtctcgtcc tcctccggcc
     2221 ccgtcgcgac ctccggtcgg cgggcccatg tcgatcgtca ccaccgatgc gtcggcgccg
     2281 tacttgatgg cgttctcgat caggttggac agcgcgctgg tgagttgacg ctggtcggcg
     2341 aacaccgacc ggtcggggtc gccgatgacc ttgagttcga tctcgcgtgc cgccgccgcg
     2401 gagtcgagtc gagcagctgc ctcggccacg atcggcccca gcacctgctc ggtgagttcg
     2461 aggtcgtcgg cgaactcgat ccgcgacagc tccaacaggt cgtcgatcgt cgcggcgagg
     2521 cgatgcactt cctcgacgat gcgcttggtg aaacgagcga cggtcgccgg gtcggtctcg
     2581 gcatcgacgg tctcggcgag caggccgagt gcccccaccg gggtcttgag ctcgtgggag
     2641 atgttcgtca cgaagtcacg gcgaacctgt tcggtacgtc ggcgttcgga gatgtcctcg
     2701 atgacgacga ccacgccctg ttgtggagct tccgacgaca cgaccagcgg ggatgcgacg
     2761 acgagatagg tcgccttcgg agggccgaac agatcgacct cacgtcgacc gctgcgcccg
     2821 gtggccgcct cggagatgag ctcgatcgtc gcctgacgca cgatcgcacg cgagtcgctc
     2881 gacgtcatga gtcgacttgc gacgccgttc tcaaaggcca cctccccgct ggggtcgacg
     2941 acgaccacac cgagttcgac cgcctcgagc gccgcgacca gtcgggcgag ttcagtcgag
     3001 gtgtcgacgt catcgacacc gggttcgacg acattcgcac gcagggcgga cagctcactg
     3061 cgctggccca gaacgatcgc gatgagcgcg acgacggcag cgacgagcac tacgaccgag
     3121 acgatgagga cgatgaccat gctgatcgga tccggggatc cggccgactc cgaaactcag
     3181 ccggccgccg gt
//
LOCUS       DAOOMA010000287         3184 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_36929_length_3184_cov_4.042825, whole genome shotgun sequence.
ACCESSION   DAOOMA010000287 DAOOMA010000000
VERSION     DAOOMA010000287.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 3184)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3184)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3184
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36929_length_3184_cov_4.042825"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..462)
                     /locus_tag="PLP95_08325"
     CDS             complement(<1..462)
                     /locus_tag="PLP95_08325"
                     /inference="COORDINATES: protein motif:HMM:NF012398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HPB45842.1"
                     /translation="MAFPQPGGEASPIKLKSRYEHYIGGAWVPPANGLYFENPSPVDG
                     KVFCEVARGTAADVEAALDAAHGARATWGATSPAERSAALLAIAQKMEDHLEELAVVE
                     TWENGKPVRETLAADLPLAIDHFRYFAGVLRAEEGGISQLDDTTVAYHFKEP"
     gene            592..1779
                     /locus_tag="PLP95_08330"
     CDS             592..1779
                     /locus_tag="PLP95_08330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB45843.1"
                     /translation="MASAELWSRRREIESARAAGRAIDPVIDGSWQRCKPMLPASRAH
                     APVDAEPDEVGERWEASPIRRSGIGVEAQLASAAEASDLIAAITDEEGRILWTAGSTR
                     MRDLAAAVGFVPGGRWDEASAGTNALGLALLTGRPASVFSAEHWCQSVQDWVCWSVPV
                     RAPDGRRLGVLDLSGKWDRASPVAALAVASLARLVEEHLPSGVVAPGAEDGLTLRLLG
                     QAGATFRGQALALSPRQSELLAALALEGPCSLDRLSELVYGDRQVRAATVKAEISHLR
                     RLLGGGIASRPYRLTVPVRVDVTELRQMLRDGDLRGATLTYAGQMLPNSESPFAVDHR
                     HVVDVMLREALLSAGSADELLRFAEVHRYDEAVLERAMAVAAAHPAARHEAEARLELA
                     RRG"
     gene            1886..>3184
                     /locus_tag="PLP95_08335"
     CDS             1886..>3184
                     /locus_tag="PLP95_08335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB45844.1"
                     /translation="MRDCAADRATAWTGRGALPRPAVDRAVDGSAPFVALVGPAGFGK
                     TVALNAIAQRHDGLAVVSARVGRAALGEALQGGIDRGLVLLDDAHLLEPERSCDLVRL
                     VERVVAQGGRLVLASRTTPPLPLRRWQVEGTAEVVSASGLAFDHATLSGLFEVDVVEI
                     DRIMGATHGWPSAVALLAQRMNEMPFEAALRVMHPQHVSYVAEEVLGVLDDDERDLLR
                     TLAVLDHLDGEVVGRVLGDRRGLKVLWDIADRTQLFESVSPGQLGWRGDVRAALMHEL
                     LIERPESVCGLHLAAAEALGMQRRHVAARLGHLIQAGAWDLGLDLLVAHWREMLDPDQ
                     FDVLADAAAAMPPDTVASDPVLQLGTGAVLLVAGHTATAKLYFDEATAHGGPEVAATV
                     AALLAHGTWWSTPPSHALDHLDEVARFFDGEAHRELLSIPG"
BASE COUNT          443 a         1033 c         1161 g          547 t
ORIGIN      
        1 cggttccttg aagtggtagg cgaccgtggt gtcgtcgagt tgggagatac cgccctcctc
       61 ggcgcgcagc accccggcga aatagcggaa atggtcgatg gcgagcggca ggtcggcggc
      121 aagggtctct cgaaccggct tgccgttttc ccaggtctcg acgacggcga gttcctcgag
      181 atggtcctcc atcttctggg cgatggccaa caacgctgcg gaacgttcag ccggactggt
      241 cgcaccccac gtcgcccgtg cgccgtgagc ggcatccagt gccgcctcga cgtcggcagc
      301 ggtgccccga gctacctcgc agaagacctt gccgtcgacc ggcgacgggt tctcgaagta
      361 caagccgttc gccggaggaa cccacgctcc gccgatgtag tgctcataac ggctcttgag
      421 cttgattggg cttgcttcgc ccccgggctg tggaaacgcc atgttgaaac cccctgttca
      481 gcgtgatcgc gccccctgcg cgaccgcttc gcacagcatg ggcttggtag gttggcaaag
      541 ggttggtgcc gatgatcggg ggtgaggcat cgcccgctgt gaggaggagc catggcgagc
      601 gcagaactgt ggagccggcg tcgggagatc gaatcggcgc gcgccgccgg ccgagccatc
      661 gacccggtca tcgacgggtc gtggcaacgc tgcaagccga tgttgccggc gagccgtgca
      721 cacgcaccgg tcgacgcgga gccggacgaa gtcggcgaac ggtgggaggc gtcgccgatt
      781 cgccgcagcg gtatcggggt ggaggcacag ctcgcctcgg cggcggaggc gagcgatctc
      841 atcgccgcga tcaccgatga ggagggccgg atcctgtgga ccgccgggag cacgcggatg
      901 cgtgatcttg ccgcagccgt gggattcgtc ccgggtggac gttgggacga ggcatcggcg
      961 ggcaccaacg cgctcggcct ggcgctgctc accggccgcc cggcatcggt gttttccgcc
     1021 gagcactggt gtcagtctgt ccaggactgg gtgtgttggt cagtgccggt gcgcgccccc
     1081 gacggtcgtc gcctcggcgt gttggacctg tcggggaagt gggatcgggc atcgccggtg
     1141 gcggcccttg cggtggcctc gctcgctcgt ctcgtcgagg agcacctgcc gtcaggtgtt
     1201 gtggcgccgg gcgccgagga cgggttgacg ttgcgtctgt tgggccaggc cggtgcgacc
     1261 tttcgcgggc aggccctggc gctttctccc cggcagtcgg agttgctcgc ggcgctcgcg
     1321 ctcgaaggcc cgtgcagcct cgaccgtctg agcgagctgg tgtatgggga tcgccaggta
     1381 cgcgccgcga ccgtgaaggc ggagatctcg cacctgcgtc gtctgctcgg cggcggcatc
     1441 gcctcgcgcc catatcgcct gaccgtgccg gtgcgtgtgg acgtgacgga attgcggcag
     1501 atgctgcgcg acggcgacct tcgcggcgcg accctgacct acgccggtca gatgttgccc
     1561 aacagcgagt ctccgttcgc ggtcgaccac cgtcatgtgg tggatgtgat gttgcgggag
     1621 gctctgctct cggccggcag cgcggacgag ttgttgcggt tcgccgaggt ccaccggtac
     1681 gacgaggcgg tgctcgagcg ggcgatggcg gttgccgcgg cccaccccgc ggcgcgccat
     1741 gaggcggagg cgagactcga acttgcccgg cgcggctgac acgttgggtg cgatccaccg
     1801 gtcggccgag cgttgatgca gcgtgatcgc ttcaccggcg tctcctgccg gcccaggtca
     1861 gggtcgagcg tgggatcatg atgagatgag ggattgtgca gccgatcgtg cgacggcctg
     1921 gaccggacga ggtgcgctgc ctcgacctgc ggtcgaccgc gccgtcgacg gatcggcgcc
     1981 gttcgtggcc ctcgtcggtc ccgcagggtt cggcaagacc gttgcgctga atgcgatcgc
     2041 gcaacgacac gacgggttgg ccgtcgtcag cgctcgcgtc ggacgagcag cactcggtga
     2101 ggcgctgcag ggcggcatcg accgaggtct ggtcctgctc gacgacgcac acctgttgga
     2161 acccgagcgg tcgtgtgact tggtccgcct cgtcgaacgg gtcgtcgccc aaggtggccg
     2221 gctggtgctc gcgtcgcgaa cgacgccgcc actgccgctg cgtcggtggc aggtcgaggg
     2281 caccgcagag gtggtgtccg catccgggct ggcattcgac cacgcgaccc tctccggcct
     2341 gttcgaggtc gacgtcgtcg agatcgatcg gatcatggga gcgacacacg gttggccctc
     2401 cgctgtcgcc ctgctcgctc aacgcatgaa cgagatgccc ttcgaggcgg cgctgcgggt
     2461 gatgcatcca cagcacgtgt cctacgtggc cgaggaggtg ctcggggtgc tcgacgacga
     2521 cgaacgcgac ctgctgagga cgctcgccgt cctcgaccac ctggacggag aggtggtggg
     2581 ccgagtactc ggcgaccgcc gcgggctcaa ggtgttgtgg gatatcgccg atcgtacgca
     2641 gctgttcgag tcggtgtcgc ccggacaatt gggatggcgc ggcgatgtcc gcgcggcgct
     2701 gatgcacgag ctgctcatcg aacgcccgga gtcggtttgc ggacttcacc ttgccgcggc
     2761 cgaagcgctc ggcatgcaac gccgtcacgt cgcggcccgg ttgggccacc tcattcaggc
     2821 cggggcatgg gacctcgggt tggacctact ggtcgcccac tggcgggaga tgttggaccc
     2881 cgaccagttc gacgtccttg ccgacgcggc cgcggcgatg ccgcccgaca ccgtcgcgtc
     2941 ggaccccgtc cttcagctcg gcaccggcgc ggtgctcctc gtggcgggcc ataccgcgac
     3001 cgccaagctc tacttcgacg aggcgaccgc tcatggcggc cccgaagtcg cggccaccgt
     3061 ggcagcgttg ctcgcccacg gcacctggtg gtcgacgcct ccgagccatg cgctcgacca
     3121 cctcgacgag gtggcgcggt tcttcgacgg cgaggcgcac cgggagttgc tcagcattcc
     3181 tgga
//
LOCUS       DAOOMA010000288         3175 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_37063_length_3175_cov_5.232692, whole genome shotgun sequence.
ACCESSION   DAOOMA010000288 DAOOMA010000000
VERSION     DAOOMA010000288.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 3175)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3175)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3175
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37063_length_3175_cov_5.232692"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(130..582)
                     /gene="rplI"
                     /locus_tag="PLP95_08340"
     CDS             complement(130..582)
                     /gene="rplI"
                     /locus_tag="PLP95_08340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012223471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HPB45845.1"
                     /translation="MINVKLVLRDNVDGLGKRGDVVEVAPGYARNYLVPRGMAIVAND
                     GIEAQAEAMRRSAALKDSRDREQAEDVAKGLVSRTITIKARAGSGGKLFGSITQTEIV
                     AAIADQAGIEIPRKAVRLDEHIKNTGDHSVQVRLHHDVEFPVSIQVVS"
     gene            complement(735..983)
                     /gene="rpsR"
                     /locus_tag="PLP95_08345"
                     /pseudo
     CDS             complement(735..983)
                     /gene="rpsR"
                     /locus_tag="PLP95_08345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983142.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
     gene            complement(983..1465)
                     /gene="ssb"
                     /locus_tag="PLP95_08350"
     CDS             complement(983..1465)
                     /gene="ssb"
                     /locus_tag="PLP95_08350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012223475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HPB45846.1"
                     /translation="MANGNNVDLVGNVTRDPELRFTPSGQAVATFGLAVNRRWQNRQN
                     QQWEESTSFFDIVCWGQLGENVAESVARGTRLIVTGRLEQRSWESQEGEKRSKIEVIA
                     DEVGPSLRWATASVIRNERQGGGSFDGGGGGGGGSFNAAPAPTPNAPAATYDYEEEPF
                     "
     gene            complement(1478..1813)
                     /gene="rpsF"
                     /locus_tag="PLP95_08355"
     CDS             complement(1478..1813)
                     /gene="rpsF"
                     /locus_tag="PLP95_08355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012223476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HPB45847.1"
                     /translation="MRAYELMVIIDGDVEDAGVTAVNTRVAELVEADGGSIAKTDLWG
                     RRRFAYPINHKNEGIYVVFEISTPASNLDELDRFLRLADDVVRHKVIRLPEDEAERRG
                     LLGSAAAAD"
     gene            complement(2026..2907)
                     /gene="panB"
                     /locus_tag="PLP95_08360"
     CDS             complement(2026..2907)
                     /gene="panB"
                     /locus_tag="PLP95_08360"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020378985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HPB45848.1"
                     /translation="MSLSPNTESRRPVTVPAVASTKVRNGADPMVMVTAYDAPGARIA
                     DAAGVDMILVGDSLAMVVLGYDDTLQVTVADMAHHVAAVARAKPKALIVGDLPWMSYH
                     VSASETVRNAAELIRAGAQCVKLEGGVKRVAMIEAIVDAEIPVMGHIGLTPQSVHAMG
                     GFKVQGRESQAALQLIADAKALQHAGCFAIVIEGVPDEVAAMVTDALDVPTIGIGAGP
                     RCDGQVLVYHDILGIEDRMLPKFVRRYADISGASVDAIEAFAADVRSGAFPNAEESYH
                     LSAEQAEAMALYGSVPA"
BASE COUNT          546 a         1030 c          996 g          603 t
ORIGIN      
        1 ctggggacaa gtctgacagt gaccaccgac agtgagggcg cgtaaacgcg aaggtgcccc
       61 gaccggcaac gccggcggga caccttcgtc ggagtgtgct cgctcggatc taccgagcga
      121 tgcggtggat caggaaacga cctggatcga caccgggaac tcgacgtcgt ggtggaggcg
      181 aacctgcacc gaatgatcgc cggtgttctt gatgtgctcg tcgaggcgaa ccgccttgcg
      241 aggaatctca attccagcct gatccgcgat cgccgcgacg atctcggtct gggtgatcga
      301 accgaacaac ttgccaccgg aaccggcgcg agccttgatg gtgatcgtgc ggctcacgag
      361 gccctttgcc acgtcctcgg cctgctcgcg atcgcggctg tccttcaggg cagccgaccg
      421 tcgcatcgcc tcggcctgag cctcgatgcc atcgttggcc acgattgcca tgccgcgggg
      481 aacgagatag ttgcgggcat agcccggagc cacctcgacg acgtcgccac gcttaccgag
      541 gccgtcgacg ttgtcgcgaa gtaccagttt cacgttgatc attccgctgc ctccgccgtc
      601 tgtgactcct cggcggcctc ggactcagcc gttgcatcct cgttctcgat cgcctcgaac
      661 tcggggctct cctcgttgcg gcgcccacgg cgatcgccgt cacgaccgcg gccaccacgc
      721 tgagtggtga ctcggctggt gtacggcaac aacgccattt cgcgggcgtt cttgatcgcc
      781 cgggcgatct cgcgctgctg ctgggtgtcg ttaccactca cccgacgggc gcggatcttg
      841 gcacggtccg agacgaatcg acgaagaagg ttgacgtcct tccagtcgat gtactcgatc
      901 tgctcggaaa cgagcgggct gacctttttc ttgccaccgc gtcgcgcgtt gtcgcgaggc
      961 ttggtggaac gcttgggctt catcagaacg gctcctcttc gtagtcgtag gtggcagcag
     1021 gggcattcgg tgtgggtgcg ggtgcggcgt tgaacgaacc gccgcctcct ccaccgccac
     1081 catcgaacga accgccgcct tgacgctcgt ttcgaatgac agatgcggtc gcccagcgca
     1141 ggctggggcc gacttcgtcg gcgatgacct cgatttttga tcgcttctcg ccttcctggc
     1201 tttcccagga tcgctgctcg agacgaccgg tcacgatcaa gcgagtaccg cgagcgacgc
     1261 tctcggcgac gttctcaccg agttggcccc aacagacgat gtcgaaaaac gacgtcgatt
     1321 cttcccattg ctggttctgt cggttctgcc aacgacggtt gacggcgagg ccgaacgtgg
     1381 cgaccgcttg ccccgagggg gtgaaacgaa gttccgggtc gcgagtgaca ttgccgacga
     1441 ggtcgacatt gtttccattg gccatgactg aagctcctta gtctgcggct gcggctgagc
     1501 cgagcagacc acgtcgctca gcctcgtcct cgggcagccg gatcaccttg tggcggacaa
     1561 cgtcgtccgc gagacgcagg aagcggtcaa gttcgtcgag gttggacgcc ggagtcgaga
     1621 tttcgaacac gacgtaaatg ccctcgttct tgtggttgat cggataggcg aagcgacgac
     1681 ggccccacag gtccgtcttg gcgatcgagc cgccatcggc ttccaccagt tccgcaaccc
     1741 tcgtgttgac ggcggtgact ccagcgtctt cgacgtcgcc gtcgatgatc accatgagtt
     1801 cataagcgcg catgtatgag gtacctccct gtggacctcg gcaggccgag ggccccggac
     1861 ggtccggggc agggtgtctg tgaacagact ggtcgagact actcgccggg aatggtcggc
     1921 caaaaattcg gtgacgacct cgacgaatga ttcgacgaaa cggtgacgtg cttcctggtg
     1981 cccatcattg ccggaaccac cgacagcgac gccgcgagcc tcgactcagg cgggcacact
     2041 cccgtacaac gccatcgctt ctgcctgttc ggcgctgagg tgatagctct cctccgcatt
     2101 cggaaatgct ccggagcgaa catctgcggc aaaggcctcg atcgcgtcga cggatgcacc
     2161 ggagatgtcg gcatagcggc ggacgaattt gggcaacatc cgatcctcga tgcccaagat
     2221 gtcgtgatag acgaggacct ggccgtcaca acgcggcccg gctccgattc cgatggtcgg
     2281 aacgtcgagc gcgtcggtca ccatcgccgc tacctcgtcg gggactccct cgatcacgat
     2341 cgcgaaacac ccggcgtgtt gcaacgcctt cgcatccgcg atcaattgca gtgccgcctg
     2401 cgactcgcgg ccctgaacct tgaacccgcc catggcatga accgactgcg gcgtcagacc
     2461 gatgtggccc atcaccggaa tctcggcatc gacgatcgcc tcgatcatcg ccacgcgctt
     2521 cactccgccc tcaagcttca cgcactgggc acccgcacga atcaactccg cagcgttgcg
     2581 cacggtctcc gaggccgaga cgtgatagct catccatggc agatccccca cgatgagcgc
     2641 cttcggcttg gcccgagcga ccgcggcgac gtgatgggcc atgtcggcca cggtgacctg
     2701 gagcgtgtcg tcgtaaccga gcaccaccat tgcgagcgag tcgccgacga gaatcatgtc
     2761 gacgccggcc gcgtcggcga tgcgcgcccc gggggcgtca taggcggtga ccatgaccat
     2821 gggatcagca ccgttgcgca ccttcgtgga cgcgaccgcg ggaacggtga cgggtcggcg
     2881 agactcggtg ttgggtgaaa ggctcatggt gagcacctcc tggttatgag gtccggtgcc
     2941 cttcagctac cggccgataa acgcactcta aaacgacttg acctgtcagc gctaggggtg
     3001 attttgctcg tccggttcca cgtcgaggtt ctccccccgt cgctgctcag ggatccgtcg
     3061 ccgcgcccgc gctctcgccc gccggggccg ccggctgcgc cgccactgtg gcaccgggag
     3121 tcgtgaccgg tgcggattcg ggcggaattt ccggtggagc cgtcgtcgtg gtcgt
//
LOCUS       DAOOMA010000289         3172 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_37107_length_3172_cov_3.739814, whole genome shotgun sequence.
ACCESSION   DAOOMA010000289 DAOOMA010000000
VERSION     DAOOMA010000289.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 3172)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3172)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3172
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37107_length_3172_cov_3.739814"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1349)
                     /locus_tag="PLP95_08365"
     CDS             complement(<1..1349)
                     /locus_tag="PLP95_08365"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: protein motif:HMM:NF013180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071111 - cyclic-guanylate-specific
                     phosphodiesterase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing phosphodiesterase"
                     /protein_id="HPB45849.1"
                     /translation="MPSSALGLPDLVAAMPAATAVIDQWGRTVTANQAWLPLAGDVAP
                     LFGAATTAAGVSGAGEPESVRLTGAAAQVRAVLQGERDAVEVDALVGGREMLLQVVAI
                     GEGASYSLAVITPVHLPAPAPPAAPAPPVAPMADVTPNPTVQSAAGVDQEPSEVDATL
                     RTNNGALPGRELFCELLAQASTMSARHGFIPVVLAVVIDGLDHVEANHGAEARRDVLV
                     AMASRFKSGLRPSDFLAQPDSESFLVLLPDVDSIETAEHIAHRLCVEQSQPFRVGDRR
                     LHVSIRVGIAGADPRKSAEAVVSAATEALDATTPLPDHAVIDLTGVEPGGERDELRVV
                     SVANAQRRRDSLPRPVRKIDLREMKDEELISYYQPIFDVRNDRVVAGEALMRWRHPHY
                     GVLSAGEFLGLAINASMLATLTDQALVRATETWAEIRDGFGPVPPRLFVNLSPEQLL"
     gene            complement(1494..2780)
                     /gene="lysA"
                     /locus_tag="PLP95_08370"
     CDS             complement(1494..2780)
                     /gene="lysA"
                     /locus_tag="PLP95_08370"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HPB45850.1"
                     /translation="MSRFATYLLPDNASVDSEGQLCIGGLRATDLAEEFGTPLFVYDE
                     DHLRMRMREAVAAFGPGVAYASKAFLCGAVARMAHEEGMKIDVASGGELFVALRAGVP
                     GERIVLHGNNKSDAELIEAIEANVSKIVVDSFDEFDRLERLLGPGGTHESFAPQPIMI
                     RVTPGIEVHTFDEVQTGQVDSKFGFGWGSGDAHRAAQRANDSGAVELLGLHVHIGSQV
                     FSVDNFASAIEMVAPLAKELAAEEFCVGGGLGVAYVDGEEGSTISAWAAAVKRVCAKA
                     GIDADVTAEPGRSIVAQAGMTLYRVGTIKRIPGVRTYVAVDGGMSDNPRPVLYGSGYE
                     TFLPRAVTASRPDVVRVVGKHCESGDVLVREAALPADLAVGDIVCTPVTGAYGHSMGS
                     NYNKVPRPAVVFVSRGSARLVVRRETYDDLTRLDLE"
     gene            complement(2777..>3172)
                     /locus_tag="PLP95_08375"
     CDS             complement(2777..>3172)
                     /locus_tag="PLP95_08375"
                     /inference="COORDINATES: protein motif:HMM:NF017555.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DALR anticodon-binding domain-containing
                     protein"
                     /protein_id="HPB45851.1"
                     /translation="IVAPPLESVELGVLVHGRELEVLRGLNSLPQVIELAARDRAPHK
                     VTTWLREFAAEVHGWYHDCPVLRDDVDEATRSARLWLAEAALVGLRVGLSVLGATAPD
                     QMAFLDAEADAANDDATADGAGGDAGTPS"
BASE COUNT          533 a         1133 c         1024 g          482 t
ORIGIN      
        1 ctcaacagct gttcggggct gaggttcacg aacaaccgcg gcggcacggg cccgaaccca
       61 tcgcggattt ccgcccaggt ctcggtggca cgcaccaggg cctgatcggt cagggtggcc
      121 aacatcgagg cgttgatcgc cagcccgagg aactcgccgg ccgacagcac cccatagtgc
      181 gggtggcgcc agcgcatcag cgcctcacct gcaacgacgc gatcgtttcg gacgtcgaag
      241 atcggctggt agtagctgat caactcctcg tccttcatct cccggagatc gatcttgcgc
      301 accggcctcg gcagggagtc gcgacgacgc tgcgcgttgg ccaccgacac gacgcggagc
      361 tcgtcgcgct ccccgcccgg ttcgaccccg gtcagatcga tcacggcgtg gtcgggaagc
      421 ggcgtggtcg catcgagtgc ctcggtcgct gcggacacga ccgcctccgc ggacttgcgc
      481 gggtcggcac cggcgatgcc gacacgaatg ctgacgtgga ggcgacggtc gcccacgcga
      541 aacggttggg attgttcgac gcagagccgg tgagcgatgt gctccgcggt ctcgatcgag
      601 tcgacgtcgg gcaacagcac cagaaacgac tcgctatcgg gttgagcgag gaagtcgctc
      661 ggccggagcc cggacttgaa cctcgacgcc atcgcgacga gcacgtcgcg gcgcgcctca
      721 gccccgtggt tcgcctcgac gtggtcgagg ccgtcgatga cgaccgccaa cacgaccgga
      781 atgaacccgt gacgagccga catcgtcgaa gcctgcgcca acagttcaca gaagagttcg
      841 cgacccggca gcgccccgtt gttggttctg agggtggcgt cgacttcgga cggttcctga
      901 tcgactccgg cggcggactg cacggtcggg ttcggcgtga cgtcggccat cggcgccacc
      961 ggaggtgccg gagccgccgg aggtgccggt gctggtaggt gaaccggcgt gatcaccgcc
     1021 aacgagtagg acgctccttc accgatcgcg acgacctgca gcagcatctc gcggccgccg
     1081 acaagcgcat cgacctcgac ggcatcgcgc tcgccctgca acacggcacg gacctgagcc
     1141 gccgcgccgg tcaaccgcac cgattcgggc tcaccagcgc cggaaacacc cgccgcggtc
     1201 gttgccgccc cgaacaacgg cgcgacgtcg ccggcaagcg gtagccacgc ttggttcgca
     1261 gtgacggtgc ggccccactg atcgattacg gcggttgcag caggcatggc tgccaccagg
     1321 tcgggaagac ccaatgcgct gctcggcact catgctcctt cgaggtggtg cggcacgaag
     1381 ccgcgtcgtc gttcgaacca ccggtggcga ttcgggtcga gcctaggtcc cgacgccgtc
     1441 gcccgcaggg accccgaccg catggacccc ggccgcaggc gaccgcccga cgatcactcg
     1501 aggtcgagcc gcgtcagatc gtcataggtc tcgcgtcgca ccacgaggcg agccgatccc
     1561 ctcgacacga acaccaccgc cggacgcggg accttgttgt agttggaacc catcgagtgt
     1621 ccgtacgctc cggtcaccgg cgtgcacacg atgtcgccca ccgcgaggtc ggccggcagc
     1681 gccgcctcgc gaaccaggac atcgcctgat tcgcagtgct tgccgacgac gcggaccacg
     1741 tcgggtcgcg acgcggtcac cgctcgggga aggaacgtct catatcccga cccgtacaac
     1801 accggacggg ggttgtcgct catcccgccg tcgaccgcca cataggtgcg cacccctgga
     1861 atgcgtttga tggtccccac ccggtacagc gtcattcctg cctgggcgac gatcgatcga
     1921 ccgggttcgg cggtgacatc ggcgtcgatg cccgccttgg cacacacgcg cttgaccgcc
     1981 gccgcccacg ccgagatcgt cgaaccctct tccccgtcga cataggcgac gccgaggccg
     2041 ccaccgacgc agaactcctc cgccgcgagc tccttcgcca gcggagccac catttcgatc
     2101 gccgacgcga agttgtcgac cgaaaagacc tggctaccga tgtggacgtg aagtccgagc
     2161 aactcgaccg cgccggagtc attcgcccgc tgagccgctc gatgagcgtc gccgcttccc
     2221 cagccgaaac cgaacttcga gtcgacctgt cccgtctgca cctcgtcgaa ggtgtgcacc
     2281 tcgatgcccg gggtcacccg gatcatgatg ggttgcgggg cgaaggactc atgtgtgccg
     2341 ccggggccga gcagtcgttc cagtcggtcg aactcgtcga agctgtcgac gacgatcttc
     2401 gacacgttcg cttcgatcgc ctcgatcagc tccgcgtcac tcttgttgtt gccgtgcagg
     2461 acgatgcgct cccccggaac cccggcgcgc agcgccacga acaactcgcc accggaggcg
     2521 acgtcgatct tcatcccctc ctcgtgggcc atccgggcga ccgctccgca caggaacgcc
     2581 ttcgacgcgt acgcgacccc cgggccgaac gccgcgaccg cttcgcgcat gcgcatccga
     2641 aggtggtcct cgtcgtagac gaacagcggg gttccgaact cctcggcgag gtcggtcgcc
     2701 cgcaatccac cgatgcacag ctgtccctcg gagtcgaccg aggcgttgtc ggggagcagg
     2761 taggtggcga agcgactcac gacggtgtgc ccgcgtcgcc gcccgcccca tccgccgtcg
     2821 cgtcgtcgtt cgccgcgtcg gcctcggcgt cgaggaacgc catctggtcg ggggccgtcg
     2881 ctccgagcac cgagagcccg acccgcaacc cgacaagtgc ggcctcggcg agccacagtc
     2941 gggcggagcg cgtcgcttcg tccacatcgt cgcgaagaac cgggcagtcg tgataccagc
     3001 cgtgcacctc cgcggcgaac tcgcgaagcc aggtcgtgac cttgtgaggg gcccgatcgc
     3061 gggccgccaa ttcgatcacc tgaggaagcg agttcagtcc tcgcagcacc tcgagttcgc
     3121 ggccatgaac cagcacgccg agctcgacgc tttcgagcgg gggcgccacg at
//
LOCUS       DAOOMA010000290         3160 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_37291_length_3160_cov_4.730435, whole genome shotgun sequence.
ACCESSION   DAOOMA010000290 DAOOMA010000000
VERSION     DAOOMA010000290.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 3160)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3160)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3160
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37291_length_3160_cov_4.730435"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..194
                     /locus_tag="PLP95_08380"
     CDS             <1..194
                     /locus_tag="PLP95_08380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HPB45852.1"
                     /translation="PTEIVQYANTRGADRIMYGGYFPMGLSLERIFGELPAVGFRDEV
                     WPEFLYGNAARVLRIEGPS"
     gene            191..1387
                     /locus_tag="PLP95_08385"
     CDS             191..1387
                     /locus_tag="PLP95_08385"
                     /inference="COORDINATES: protein motif:HMM:NF012656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HPB45853.1"
                     /translation="MTNENDEQGATQRSTSERDAFRAEVRAWLNANAPAKGSAEDFSA
                     IHVVSAPTREEFESRERRALEVTRAWQRRLFDAGWAGRSWPVEYGGQGAPSWQSEIIA
                     EEAAQFGVSTKMLSIALEMVPPVLLRFGTDDQRLRHLPHIVRGDQSWCQLLSEPDAGS
                     DLTNVQTLATPTEGGWTINGQKVWTSGAVSADYAILIARTDRSIAGRGGLSCFALDMN
                     QPEVVVRPLRQMSGGYHFNEVFLNDAFVPHDALIGNLGGGFDVLRVMLTSERSAIGGG
                     TSARSSTALQQLARDLGLDRDPIVRQNLARAHTREKLLDLVGARIAAIPAGASVAKLS
                     YSEHARLSANAAMDLLGARGMVTDDEHAEAFADRFLFAPGLRLGGGTDEIQRNTIAER
                     GLGLPR"
     gene            complement(1447..2847)
                     /locus_tag="PLP95_08390"
     CDS             complement(1447..2847)
                     /locus_tag="PLP95_08390"
                     /inference="COORDINATES: protein motif:HMM:NF016232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPB45854.1"
                     /translation="MVPGDVDRALALPPDEAAERLSVLPEDQWFERKAGRISARDLAV
                     PLIAMANAEGGYVAVGIHDGVVDGVAAPHINALRQAAQDFTSPVVRCTATEFEVSDGR
                     VVLVFRIDPGEHVHETTKGDVYLRVGDESRKLGYSQRRELEFDRGSTPFDGTAVEAHV
                     DDLDQNQVGAYQTSIGSQTPRDMLAARDLLTRDGRLTVAGWLLFAERPQSLFPSASVR
                     VLRYAAVRRGTGSAMSLYEGGDTRCVGSIPEQISQAAEVIERWVPKVEALARSGRFEP
                     RSIIPRDVWLEGLVNAVLHRSYSMAGDHVRVEIFPNRIEIENPGRFPGLADPSEPLSI
                     SRYARNPRIVRVCSDLGIARELGEGIRRIFADMRMLGLADPIYTQGSGSVRLVLSAAD
                     ALDETVRASLSKAARQILDVLRLEGRPLSTGQVADLAGVARPTASRHLQTLRDLGLVD
                     WDGQSPRDPRAAWTLR"
BASE COUNT          601 a         1036 c          991 g          532 t
ORIGIN      
        1 accccaccga gatcgtgcag tacgccaaca cccgcggcgc cgatcgaatc atgtacggcg
       61 gctacttccc gatgggcctc agcctcgaac gcatcttcgg ggaactgcca gcggtcgggt
      121 ttcgcgacga ggtgtggccg gagtttctct acgggaacgc cgcccgggtt ctccgcatcg
      181 aaggaccgtc atgacgaacg agaacgacga acagggcgcg acgcagcgct ccaccagcga
      241 gcgcgacgcc ttccgtgccg aggtcagggc ctggctcaac gccaatgcgc cagccaaggg
      301 cagcgccgag gacttctcgg cgatccacgt cgtgtcggct ccgacccgcg aggagttcga
      361 gtcgcgcgaa cgccgcgcgc tcgaggtcac gcgcgcctgg caacggcgcc tcttcgacgc
      421 agggtgggcc ggcaggtcgt ggcccgtcga gtacggcggc cagggcgcac cgagttggca
      481 aagcgagatc atcgccgagg aagccgcgca gttcggggtg tcgaccaaga tgttgtcgat
      541 cgccttggag atggtgccgc cggtgctgtt gcgcttcggc accgacgacc aacgcctgcg
      601 ccaccttccc cacatcgtgc ggggcgacca gtcgtggtgt cagttgctgt ccgagcccga
      661 cgccggcagc gatctaacca acgtgcagac actcgccaca ccaaccgaag gcgggtggac
      721 gatcaatggc caaaaggtct ggacctccgg ggcggtgagt gctgactacg ccattctcat
      781 cgcccgcacc gaccggtcga tcgccggccg tggtgggttg agctgtttcg cactcgacat
      841 gaaccaacct gaggtcgtcg tgcggccgct gcgccagatg tcgggtggat atcacttcaa
      901 cgaggtgttc ctcaacgacg cattcgttcc tcacgacgcg ttgatcggca acctcggcgg
      961 tggtttcgac gtcttgcggg tcatgttgac gagcgagcgt tccgcgatcg gcggcggcac
     1021 gagcgcacgg tcatcgaccg cactgcaaca gctcgcccgc gacctcggcc tcgaccgcga
     1081 tccgatcgtc cgccagaacc tcgcccgagc gcacacacgc gagaagctcc tcgatctcgt
     1141 cggcgcacga atcgcggcga ttcccgcagg agcctcagtc gcaaagctct cctactccga
     1201 acatgcccgg ctcagcgcca acgccgcgat ggacctgctg ggcgcacgag gaatggtgac
     1261 cgacgacgag cacgccgagg ccttcgctga ccgcttcttg ttcgcccccg gactccgcct
     1321 cggcggcggc accgacgaga tccagcgcaa caccatcgcc gagcgagggc ttggcctccc
     1381 acgctgacgt tcagcgtcta ctgcaacagg tcctggaaca tctactgaaa caaatgttcc
     1441 agtaggtcag cgcaacgtcc acgcagcgcg tgggtcgcga gggctctgcc cgtcccagtc
     1501 gaccagtccg aggtcacgaa gcgtttgcag atgccgagat gcggtcgggc gcgccacccc
     1561 ggcgaggtcg gccacctgcc cagtgctcag cggccggcct tcgaggcgaa gcacgtcgag
     1621 aatctggcgg gcagccttgc tcaggcttgc gcggactgtc tcgtcgaggg cgtctgcagc
     1681 agacagtacg aggcggaccg atccactgcc ttgggtgtag atcgggtcgg cgaggccgag
     1741 catccgcatg tcggcgaaga tgcgtcgaat gccctcgccg agttcacggg caattccaag
     1801 gtcggagcac acgcgaacga tacggggatt gcgggcatag cggctgatcg acaacggttc
     1861 ggacggatcg gcgagacccg gaaagcggcc cgggttctcg atctcaatgc gattcgggaa
     1921 gatctcgaca cgaacgtggt ctccggccat ggagtacgag cgatggagca ccgcgttgac
     1981 gagtccttcg agccagacat cccgaggaat gatcgagcgt ggttcgaatc ggccgctacg
     2041 agcgagtgcc tccaccttcg gcacccatcg ttcgatcact tctgcggcct gactgatctg
     2101 ctccgggatt gaaccaacac atcgggtgtc accgccttca tacagcgaca tcgccgaccc
     2161 ggtaccacga cgaacggccg cgtatcgcag cacacgcact gaggcgctcg gaaacagtga
     2221 ttgaggacgc tccgcaaaga gcagccatcc ggcgaccgtc aaacgaccgt cacgggtcag
     2281 gagatcgcgg gcagcaagca tgtctctggg cgtctgtgaa ccaatcgacg tctgataagc
     2341 accgacctgg ttctggtcga ggtcatcgac gtgggcctcg acagcggttc cgtcgaaggg
     2401 ggtgctgcct cgatcgaact ccagctctcg gcgttgcgaa tagccgagct ttcgcgactc
     2461 gtcgcccact cggagataga cgtctccctt ggtcgtctcg tggacatgct cgccgggatc
     2521 gattcgaaac acgagcacca cccgaccgtc cgagacctca aactcggtcg cggtacaccg
     2581 cacgacaggg gacgtaaagt cctgcgccgc ctgtcgcaac gcgttgatat ggggtgctgc
     2641 gactccatcg acaacgccgt cgtgaatgcc aacagcgacg tagccgccct cggcattcgc
     2701 catcgctatc aacggaactg caaggtctcg cgcgctgatg cgacctgcct tgcgttcgaa
     2761 ccactgatcc tcgggcaaga cgctcagccg ctccgccgcc tcgtctgggg gaagggcaag
     2821 cgcacggtcg acatcgccag gaaccatgga tctactgtaa catttttaca gtaggtttca
     2881 gtatcagccg tcgaactgtc acgcgtccga aacgctggtc gccggctgcg tgacgttgca
     2941 ggtctcgaac tccgaagaac cgaccgggta gtggatcgtc ttgcgctcgg ccaacagttc
     3001 cttgcactca gcgaaggtcc tgtagccgta atggtacgaa ctgagcgagg gcttggccag
     3061 ccgcgttggg gttcagcgtc taccacaaca cgaacgccct actgcaacaa atgttccggt
     3121 acgtcagcgc aacgtccacg cagcgcgtgg gtcgcgacgg
//
LOCUS       DAOOMA010000291         3155 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_37362_length_3155_cov_4.495806, whole genome shotgun sequence.
ACCESSION   DAOOMA010000291 DAOOMA010000000
VERSION     DAOOMA010000291.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 3155)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3155)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3155
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37362_length_3155_cov_4.495806"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..192
                     /locus_tag="PLP95_08395"
     CDS             <1..192
                     /locus_tag="PLP95_08395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB45855.1"
                     /translation="AGAAGAAGGIAGGYRLRFTAIAMVIGVGGPYSATLTCIATVGVA
                     AAAGVAVVWALDRIAIRSK"
     gene            complement(226..474)
                     /locus_tag="PLP95_08400"
     CDS             complement(226..474)
                     /locus_tag="PLP95_08400"
                     /inference="COORDINATES: protein motif:HMM:NF016141.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HPB45856.1"
                     /translation="MLIVGILLFGMFVGALAQMLLGRAGHAIDWPLALGAGLGGSFVG
                     GLLVSLLAGDGLSLRPSGLIGSFVGAVIVTAIVTRSRR"
     gene            complement(538..981)
                     /locus_tag="PLP95_08405"
     CDS             complement(538..981)
                     /locus_tag="PLP95_08405"
                     /inference="COORDINATES: protein motif:HMM:NF012931.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 20 glycosylhydrolase"
                     /protein_id="HPB45857.1"
                     /translation="MRAARRHSAGRSRRVLMSPLMHVYLDQPMVEPGPQDQEERRQRV
                     GLRLYPGNTVRSSLDWDPLTLRDDVTSEEQVAGVEAAIWCETVMSFADLQFALQPRLA
                     GLAERGWVQRAPVDWDDYSARLAWRAPAWRAASWEFFRAGSVDWR"
     gene            1077..1235
                     /locus_tag="PLP95_08410"
     CDS             1077..1235
                     /locus_tag="PLP95_08410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB45858.1"
                     /translation="MSAVTSASTSATKPGVAALSGSIDTICIGPAMASESEVALPSSG
                     YAAKTAAQ"
     gene            complement(1264..1731)
                     /locus_tag="PLP95_08415"
     CDS             complement(1264..1731)
                     /locus_tag="PLP95_08415"
                     /inference="COORDINATES: protein motif:HMM:NF014852.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 20 zincin-like fold
                     domain-containing protein"
                     /protein_id="HPB45859.1"
                     /translation="MAGTFVEDLAAHGGIRLVEGGADSPILLELTSGFDGSDSLNPTH
                     GVSPIGATADSERYRLCVTAEGVHVQGTTPEAIWRGLTSLRQLVDVGAGESSVDLPAV
                     EIVDGPRYAWRGLSLDVVRTFFTVDEVKQVIDRAGSTPRLSSGISSSTPPAAL"
     gene            complement(1872..2216)
                     /locus_tag="PLP95_08420"
     CDS             complement(1872..2216)
                     /locus_tag="PLP95_08420"
                     /inference="COORDINATES: protein motif:HMM:NF020873.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1971 domain-containing protein"
                     /protein_id="HPB45860.1"
                     /translation="MNRPSMPEGLEHVRTTNTFDNDTVPAGLLRAHHVADGVWGRLVV
                     HTGTVMFVFDDDPDHPILTGPGQAVAIPPARQHHLELDEPATFAVEFYRLASTPTPVE
                     GAETTALSGEPG"
     gene            2364..>3155
                     /locus_tag="PLP95_08425"
     CDS             2364..>3155
                     /locus_tag="PLP95_08425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB45861.1"
                     /translation="MSDFPQGPDWWQASDLKWYPPQPSTPPPAPATPPIGLSSPPDPS
                     TRAAFEAPLAAPQAPWGQQPPAPGTGQPTAGPPTAGPAGAQAPWSGQPPAYPQAPQYG
                     GAGNVTVKKKSKLPWVLGIFALVVLLGVGGCLALISATTSAIDEELSKNDATVITPDG
                     SVVEPGSSGGGAGSQEDALPIGSEVDLGNGWRIKVNSADVSPGATALVEQANTFNEPP
                     EPGKRYIIVNLSATLTSSENSEPKSPWLELDYAVFGSDGVERSSSD"
BASE COUNT          570 a         1078 c         1017 g          490 t
ORIGIN      
        1 gccggtgccg ccggtgccgc cggggggatc gccgggggat accgcctgcg attcaccgcg
       61 atcgcgatgg tgatcggggt tggtggaccg tattcggcca cgctgacctg catcgcaacc
      121 gtcggtgtgg ctgcggccgc tggcgtcgcc gtggtgtggg cgctcgaccg catcgcgatc
      181 cggtcgaagt gatgccgggc gacgcgccgc cgccagcctg ccgatctagc gccgtgagcg
      241 agtaacgatg gcggtgacga tgaccgcgcc gacgaacgag ccaatgaggc cactcgggcg
      301 aaggctcagc ccgtcgccag ccagaaggct caccaacaga ccgccgacaa acgagccacc
      361 gaggccagcg ccaagggcga ggggccagtc gattgcatgt ccggcgcggc cgagcagcat
      421 ttgagccaat gcgccaacga acatgccgaa gaggaggata ccgacgatga gcattacgac
      481 accctagcga agccaccttc tgctgtcgtt cccggggcgt tcggccatcg gcggtcgtca
      541 acgccaatcg accgaaccgg cacggaagaa ctcccatgag gctgcccgcc aagctggagc
      601 cctccaagca agccgcgcgg agtagtcatc ccaatcgacc ggggcgcgct gcacccaacc
      661 acgttcggcg agtcccgcga gtcgcggttg aagtgcgaac tgcaggtcgg cgaacgacat
      721 caccgtctca caccagatcg cggcctcgac ccctgccacc tgctcctccg aggtcacatc
      781 gtcgcggagg gtcagcggat cccagtccag cgatgaacgc accgtgttgc ccggatagag
      841 ccgaagcccg acgcgctggc gacgttcttc ctgatcctga ggcccgggct cgaccatggg
      901 ctggtcgaga tacacgtgca tcaacggcga catgagcacg cgccgactcc tgccagccga
      961 gtgtcttctt gccgctcgca ccagcgatgt cgcgcgcggc gttgacgaat cgcacgtact
     1021 tatcgtgttc cattccgaac gcttcgtcgg ccccgagatg gatgtagtca cctggagtga
     1081 gcgcggtcac ctcggccagt acgtcggcga cgaagcccgg cgtcgccgcg ttgtcggggt
     1141 ccatcgacac gatctgcatc ggccccgcca tggcctcaga gtcggaggtc gcgctgccga
     1201 gttctgggta tgcggccaag accgctgcgc agtgacccgg gagatcgatc tcgggcacga
     1261 tcgtcacaaa gcggcgggcg gcgtactcga cgagatcccg gaactcagcc tgggtgtaga
     1321 acccgcccgg tcgatcacct gcttcacctc gtcgacggtg aagaaggtcc gtaccacgtc
     1381 gagcgagaga cctcgccacg cgtagcgagg accgtcgaca atctcgaccg ccggcaggtc
     1441 gaccgacgat tcgcccgcgc cgacgtcaac cagttggcga agactggtga gcccacgcca
     1501 gattgcttcc ggcgtcgttc cctgcacgtg cacgccttcc gccgtcacac agagtcggta
     1561 gcgctccgag tccgcggtag cgccgatcgg gctcactcca tgggtcggat tcagcgagtc
     1621 ggatccgtcg aagccggagg tcagctccag caggatcggg gaatccgccc caccttcgac
     1681 gaggcggatt ccgccgtgag ccgcgagatc ctcgacgaag gttccggcca ccgacacaag
     1741 gcgatcgtcg gtccagccga tgcgttaccc cgagcggagg gtgaaggagc cgatgccttc
     1801 gtcggcacgt accggggcag gaatgattgg cagcatgtgg cgatcctgcc accccgaccg
     1861 cgtcgtcgac gtcatcccgg ttccccgctc agcgctgtgg tctcggcgcc ttccacgggt
     1921 gtcggcgtac tcgccaaccg atagaactcc acagcgaagg ttgctggctc gtcgagctcg
     1981 aggtggtgct gtcgtgcggg aggaatcgcg accgcctggc cagggccggt caggatggga
     2041 tggtcgggat cgtcgtcgaa gacgaacatg accgtgccgg tatggaccac cagccgaccc
     2101 caaaccccat cggccacgtg gtgcgcccgt agcaatccgg caggcacggt gtcgttgtcg
     2161 aaggtgttcg tggttcggac gtgctcgagg ccctcgggca tggaaggacg attcatcgca
     2221 ggctcctcac gacgcgatgt caatacccga tcgtaagcct tcatctactg gagcgaaacg
     2281 ccacgatcga aactcgaact caccgataag atgacgtcat ggcggattcc cccccgccgc
     2341 acgaagactc tcaggatggc gagatgagcg actttccgca aggccccgac tggtggcagg
     2401 catcggacct gaagtggtac ccgcctcagc cctccacgcc gccccccgca ccggcaacgc
     2461 ctccgattgg cctctcgtcg ccccccgacc cttcgacgcg cgccgcgttc gaggcgccgc
     2521 tggccgctcc ccaagccccg tgggggcagc agccgccggc tccgggcacc ggacagccca
     2581 ccgccggacc gccgaccgcc ggaccggcag gcgctcaagc cccttggtcg ggccaacccc
     2641 cggcctaccc gcaggcacct cagtacgggg gcgctggcaa cgtcaccgtc aagaagaagt
     2701 cgaagctccc gtgggtcctc ggcatcttcg cactcgtcgt gttgttgggt gtgggaggtt
     2761 gtctcgccct catctcggca acgacatcgg ccatcgacga agaactgtcc aagaacgacg
     2821 ccaccgtgat cacccccgac ggcagcgttg tcgaaccggg gtcgtcgggc ggcggtgcgg
     2881 gttcccaaga ggatgcgttg cctatcgggt cggaggtcga cctcggcaac ggttggcgga
     2941 tcaaggtgaa ctcggccgac gtcagccccg gggcgactgc cctggtcgaa caggccaaca
     3001 cgttcaatga accgccggag ccgggcaagc gctacatcat tgtgaacctg tccgccacgc
     3061 tcaccagcag cgagaactcc gagcccaagt cgccttggct cgaactcgac tatgccgtgt
     3121 tcggttccga cggggtcgaa cggagcagct cggac
//
LOCUS       DAOOMA010000292         3152 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_37412_length_3152_cov_4.288989, whole genome shotgun sequence.
ACCESSION   DAOOMA010000292 DAOOMA010000000
VERSION     DAOOMA010000292.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 3152)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3152)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3152
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37412_length_3152_cov_4.288989"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>3152
                     /locus_tag="PLP95_08430"
     CDS             <1..>3152
                     /locus_tag="PLP95_08430"
                     /inference="COORDINATES: protein motif:HMM:NF013727.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FtsK/SpoIIIE domain-containing protein"
                     /protein_id="HPB45862.1"
                     /translation="ALLEQRFPSTAALEVRAVRRTSELWDGAADDAAAPCRIGTGTVP
                     TLLNVELPADLRFAETAEELRGRYGSFRRRYEYHLDAPVTVEMQRCRAVAVVGDDATT
                     TALATSMVLQSATRLPPSRLSTAVLTTQPRATRWRWLTWLRSNAPSTWWAGSILAVAD
                     EREALLAELEELAAAGVPGATDEVERLVVVDADALGASDLARLLRVAASGVCRIVWIG
                     ERRGDAPGASEVVVVCEAADSEAADSEAAFGVVNVVEGAQQIAVRLDTVSPRRAARWA
                     RALAPLVEALPAATSAGALPRAVGLDEVFDTAVFGSTDAVAERWRTCGGRLRTPIGRR
                     GEEVVWFDLERDGPHGLIAGTTGSGKSQLLQSIVVGLAASVSPEDLSMLFIDFKGGAA
                     FADCTALPHCVGMVTDLDGHLADRVLRSLAAEIRRRERLLAAHGASDLAGYRQRPGID
                     ALARLVIVIDEFASIAAEIDGFVDAMVDIGRRGRSLGIHLLLATQRPAGVVTEHLRAN
                     VNLRIALRMADPHESENVIGIAAAATIPRSTPGRAVMRAGQGAVVLFQAASVALVDSA
                     CGDRLDPVELAPAASVGPHGAALAVHSTGPDALVSAVDTVRSAAIRGGFDAPRRPWLE
                     PVEPTLTLESLLGAHDVADAEDSVDGRPDVDVSAAPAVRLGIADEPDHQRQRVATFAL
                     DGTSLGAFGADMGSLGALVEALVLSAGHGDANGAVRFDVVDGGAALGRVEHLANVGSV
                     IDATDTERIERLLGELLARRGGTDRSGSAAHRVIVVHHLGAVLASMGRHGQRFVQSLD
                     ALMVDGRAAGVTVVLTCDRRRSMPYELFGALGARFVFAMADDAEYALVSPGADVVHRA
                     AGSLGWFEGATVQVPRRCGDSQFLELVDRHGLRGDAHPAVGRLAQHCALSDLEVDPMA
                     APWSWPIGIADATLMAATVDLQATNLLVAGPVRSGKTTALATIVHSAKHAGCHTASDP
                     VRVIVVAGRGEPDGALRAQDLDVVVAGDDIEAIAALGPTLIDTTDAVVVVVDDAHELC
                     DAAARALDGIVGDLRRSSVR"
BASE COUNT          424 a         1008 c         1218 g          502 t
ORIGIN      
        1 cggcgctgct cgagcagcgg ttcccgtcga cggcggcgct cgaggtccgg gcggtgcgtc
       61 gcacgagcga gttgtgggat ggcgccgccg acgacgcagc ggcgccgtgc cggatcggaa
      121 ccggaaccgt gccgaccctg ctgaacgtgg agttgccggc cgacctacgc ttcgccgaga
      181 cggccgagga actgcgcgga cgctacgggt cgtttcgccg acgctacgag tatcacctcg
      241 acgccccggt gacggtggag atgcagcgat gtcgggccgt cgccgtggtg ggcgacgacg
      301 caacgacgac ggcgttggcc acgtcgatgg tgttgcagag cgccacccgg ttgccgccga
      361 gccgactgtc gacggcggta ctgaccacgc agccacgggc gacgagatgg cgatggctca
      421 cgtggctgcg ctcgaacgcg ccgtcgacct ggtgggccgg ttcgatcctg gcggtggcgg
      481 acgaacgcga ggcgctgctc gccgaactcg aggaactcgc cgccgccggt gtcccggggg
      541 ccaccgatga ggtcgagcgg ctggtcgtcg tcgatgctga cgccctcggc gcgtcggatc
      601 ttgcacgact gctgcgggtg gcggccagcg gggtgtgtcg gatcgtgtgg atcggggagc
      661 ggcgcggcga cgcaccgggc gcaagcgagg tcgtcgtagt ctgcgaggcg gccgacagcg
      721 aggcggccga cagcgaggcg gccttcgggg tcgtcaacgt ggtcgaaggc gcccaacaga
      781 tcgcggtgcg gctcgacacg gtgagcccgc gccgggcagc gcgctgggcg cgggcgctgg
      841 ccccgctcgt cgaggcactc ccggcagcga cctctgcagg tgcgttgccg cgcgccgtgg
      901 ggctcgatga ggtgttcgac acggcggtct tcggatccac cgacgccgtc gctgagcgat
      961 ggcgcacctg cggcggtcgc ctccgcaccc cgatcggtcg gcggggagag gaggtggtgt
     1021 ggttcgacct cgaacgcgac gggccccacg ggttgatcgc aggaacgacc ggttcgggaa
     1081 agagccagct gctgcagtcg atcgtcgtcg gtctcgcggc gagcgtgtcg cctgaggacc
     1141 tgtcgatgtt gttcatcgac ttcaagggtg gtgccgcgtt cgccgactgc acggcgttgc
     1201 cgcactgcgt cgggatggtg accgacctgg atggccacct cgccgatcgg gtgctgcgct
     1261 cgctggccgc ggagatccgc cgccgcgaac gactgctggc ggctcatgga gcatctgacc
     1321 tggcaggcta ccgacagcgc ccggggatcg acgccttggc gcggttggtg atcgtcatcg
     1381 atgaattcgc gtcgatcgcg gcggagatcg acgggttcgt cgacgccatg gtcgacatcg
     1441 ggcgtcgagg tcgatcgctc gggatccacc tgctgttggc gacacaacgt cccgccgggg
     1501 tggtcaccga gcacctgcga gcgaacgtga atcttcggat tgcgcttcgc atggccgatc
     1561 cccacgagag tgagaacgtg atcgggattg cggctgcggc gacgatcccg cggtcgaccc
     1621 ccggacgggc ggtcatgcgg gccgggcaag gggcagtggt gttgttccag gcggcgtcgg
     1681 tcgccctcgt cgacagcgcc tgcggtgatc ggcttgaccc cgtcgagttg gctccggccg
     1741 cctcggtggg gccgcacggc gcagcgttgg cggtgcactc gacggggcca gatgcgttgg
     1801 tgtcggcggt cgacaccgtt cgttcagccg ccattcgagg ggggttcgac gcgccgaggc
     1861 gtccgtggct cgagcccgtc gaaccgactt tgacgctcga atccctgctg ggcgcacacg
     1921 acgtcgcgga tgcggaggac tcggtcgatg ggcgaccgga tgtcgacgta tcggcagcgc
     1981 ccgcagttcg gctggggatc gccgacgagc ccgaccatca gcgacagcgg gttgcgacgt
     2041 tcgccctcga cggcacgtcg ttgggagcgt tcggggcgga catgggctcc ctcggtgccc
     2101 tggtggaggc actggtgctc agcgcgggcc atggggacgc gaacggggcg gtgcggttcg
     2161 atgtcgtcga cggaggggcg gcgttggggc gggtcgaaca tctcgcgaac gtcgggtcgg
     2221 tcatcgatgc caccgacacc gaacgcatcg aacggctcct gggcgagctt ctggcgcgcc
     2281 gcggtggcac ggatcgatcg gggtcggccg cacaccgggt gatcgtcgtg catcacctcg
     2341 gggcggtgct cgcgtcgatg gggcgccacg gccaacggtt cgttcagtcg ctcgacgcgt
     2401 tgatggtgga tgggcgggcc gctggggtca cggtggtgct gacctgcgat cggcgccgct
     2461 cgatgcccta cgaattgttc ggcgcgctcg gggcgaggtt cgtgttcgcg atggctgacg
     2521 acgccgagta cgcgctggtg agtcccgggg ccgacgtcgt tcaccgggct gcaggatcgc
     2581 tcggctggtt cgaaggggca accgttcagg tgccgcgccg gtgcggcgac tcgcagttcc
     2641 tcgagctggt cgaccgccac ggacttcgcg gcgacgcgca tccggcggtc ggccggctcg
     2701 cccaacactg cgcgctgtcg gatctggagg tcgacccgat ggcagcgccg tggtcgtggc
     2761 cgatcgggat cgctgacgcg acgttgatgg ccgcaaccgt ggacctgcag gcgacaaacc
     2821 tgttggtggc cggcccggtg cgttcgggca agaccaccgc cctcgccacg atcgtgcaca
     2881 gcgccaagca cgcgggttgc cacacggcgt cggaccccgt gagggtcatc gtcgtcgccg
     2941 gacgtgggga acccgacggg gccctccggg cccaggacct cgacgtcgtc gtggctggcg
     3001 acgacatcga ggcgatcgcc gcgctcggcc cgacgttgat cgacacgacc gacgcggtgg
     3061 tcgtggtggt cgacgacgca cacgaactgt gcgacgccgc ggcccgagcc ctcgatggga
     3121 tcgtcggtga tctgcgccgg tcgtcggtgc gc
//
LOCUS       DAOOMA010000293         3145 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_37530_length_3145_cov_3.672492, whole genome shotgun sequence.
ACCESSION   DAOOMA010000293 DAOOMA010000000
VERSION     DAOOMA010000293.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 3145)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3145)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3145
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37530_length_3145_cov_3.672492"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(93..2126)
                     /locus_tag="PLP95_08435"
     CDS             complement(93..2126)
                     /locus_tag="PLP95_08435"
                     /inference="COORDINATES: protein motif:HMM:NF014156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HPB45863.1"
                     /translation="MIAELTPAGLSLSVPDSPLSIGPGRPAIDVTVGGRRSEWVAEKV
                     TSTGADTAFAKHPLGLRVEWQWNRIDDLGATPAVELSARITHDGTVPVALERFALFET
                     RSLTIGDNRKRWRTYRNGYQSWAATHTVGVSERDVDVLTTVGRRGVTDAKHPAPNTPG
                     HVRSDWISATVEPVSGHGVAAAFTTMSDAFGFIELNHHAGRAPVWSAWVDLDGTMLAP
                     GQATPWFRVVIAADEAEGSSADELLRSVAVLAGDAMEARSVDAPHPAGWCSWYYYFTK
                     VTEDDVRANLAVLAPEGRGGDTFGCEYVMIDDGHQRAIGDWLHTNDKFPSGMAALATD
                     IREAGFDAGIWWAPFICGARSEVARRHPEWLVRNERGRPIVALVNPGWGTENPMFALD
                     TTNPEVLEHLRGVASTIGVDWGYRIQKLDFLFAASLPGVRYDASATRAQSLRRGLEAI
                     REGAGDDGFLLGCGCPLGPAVGVVDAMRIGADVTPSWTTAVGRFLGRSRHGLATVHAL
                     RNTISRSVFDRAWFLNDPDCLMVRASDTTLSEAEVRTMCTVFGLTDGMIVLSDDLTTV
                     PEHRRALIRRTLELTGGNHFVADLFEVADPQVIVSVHPDRVDVGIVNLGDEPRPGRIE
                     VDRLGVAALIGSDAPTEATEFWSGRRLGVHRGVIDVGTIEPHGSVVFRFETSR"
     gene            2193..3101
                     /locus_tag="PLP95_08440"
     CDS             2193..3101
                     /locus_tag="PLP95_08440"
                     /inference="COORDINATES: protein motif:HMM:NF021982.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHB depolymerase family esterase"
                     /protein_id="HPB45864.1"
                     /translation="MTARALAIVAVLGLVAAGCGGSDSSSANPGGADNAQNAVTTTAG
                     TGDADTDGAEARVAESPGCAAAAPETPGREVRIDLASGGVERFALVTTPSAAAESVEP
                     RALVIDFHGLAEGAEVHARMSQYSELAEQEGFFAVFPQGTGDIASWKAGVGGELNPDH
                     ADVEFVTDLLERLGADHCIDLGSVYATGLSNGAMMTSTVGCLLADRFAAVAPVAGAAF
                     YNDCPSTTPMPVYAIHGTQDPLLPFNGGVGNVGAVIAGGAADQTKVEVELWHDGVPRK
                     TGATRRITDDPAHKAPKGTYQQYVHL"
BASE COUNT          514 a         1050 c         1089 g          492 t
ORIGIN      
        1 ggcgatagtc agactcaatc gtttgttcct atgtcgctga taggtcttgg ctatggccgc
       61 gaccgggtga cccgcgacct cggcaggccg cgctatcggc tggtctcgaa ccggaacacg
      121 accgaaccgt gcggctcgat ggtgccgacg tcgatcacac cccggtgaac tccaaggcgc
      181 cggccgctcc agaactcggt cgcctcggtc ggggcgtcgg agccgatcaa cgcagcgaca
      241 ccgagtcggt cgacctcgat gcgtcccggg cggggctcat cgccgaggtt gacgatgccg
      301 acatcgacgc ggtccgggtg aacgctgacg atcacctggg ggtccgccac ctcgaacagg
      361 tcggcgacga aatggttgcc ccccgtgagt tcgagggtgc ggcggatcaa cgcccgtcga
      421 tgctcgggca cggttgtgag gtcgtcggac aacacgatca tgccgtcggt caggccgaac
      481 accgtgcaca tggtgcgaac ttcggcttcg gacagggtgg tgtcgctcgc ccgcaccatg
      541 agacagtccg ggtcgttgag gaaccacgcc cgatcgaaca ccgacctgga gatggtgttg
      601 cgcagcgcgt ggaccgtggc gagtccgtgg cggctgcggc ccaggaaccg cccgaccgcg
      661 gtcgtccacg acggggtgac atctgctccg atgcgcatgg cgtcgaccac gcccaccgcc
      721 gggcccaacg ggcatccgca ccccaggaga aacccgtcgt cgcccgctcc ttcgcggatc
      781 gcctcgagcc cgcgccgcaa cgactgggcg cgggtggccg acgcgtcgta gcgaacgccc
      841 ggaagggacg ccgcgaacag gaagtcgagc ttctggattc ggtaacccca gtcgactccg
      901 atcgtcgaag cgacaccgcg gaggtgttcg agcacctcgg ggttggtggt gtcgagcgcg
      961 aacatcgggt tctcggtgcc ccaacccgga ttgaccaggg ccacgatcgg gcggccgcgc
     1021 tcgttgcgca ccagccactc gggatgacgc cgcgccacct cggaacgcgc gccgcagatg
     1081 aacggcgccc accagatgcc ggcatcgaat ccggcctcgc gaatgtcggt cgccagcgcc
     1141 gccatgcccg aggggaactt gtcgttggtg tgcagccagt cgccgatggc gcgctgatgg
     1201 ccatcgtcga tcatcacgta ctcacagccg aaggtgtcgc cgcctcgccc ttcgggggcc
     1261 agcactgcga ggttcgcccg cacgtcgtcc tcggtgacct tggtgaagta gtagtaccac
     1321 gagcaccagc ccgcgggatg tggggcatcg accgagcggg cctccatcgc gtcgccggcg
     1381 agcacggcca ccgagcgcaa cagttcgtcg gcggaggaac cctcggcctc atcggcggcg
     1441 atgacgaccc ggaaccacgg ggtcgcctgc ccgggggcga gcatcgtgcc atcgaggtcg
     1501 acccacgcgc tccacaccgg cgcacgcccg gcatggtggt tgagttcgat gaatccgaac
     1561 gcatcgctca tcgtggtgaa cgcggcggcg acgccatggc cgctgaccgg ttcgaccgtc
     1621 gccgagatcc agtcggagcg aacgtggcca ggggtgtttg gggccggatg tttggcgtcg
     1681 gtcaccccgc ggcggccgac cgtcgtcagc acgtcgacgt cgcgttcgct gacgccgacg
     1741 gtgtgggtcg ccgcccacga ctggtagccg ttgcggtacg tgcgccagcg cttgcggttg
     1801 tcgccgatcg tgagcgacct ggtttcgaac aacgcgaacc gttcgagcgc gaccggaacg
     1861 gtgccgtcgt gtgtgatgcg ggcgctcagc tcgaccgccg gcgtcgcccc gaggtcgtcg
     1921 atgcggttcc actgccactc gacgcgaagc ccgagcgggt gtttcgcgaa cgcggtatcg
     1981 gccccggtcg acgtcacctt ctcggccacc cactcgctgc gacggccacc gacggtgacg
     2041 tcgatcgcgg gccggccggg cccgatcgac agtggggagt cagggacgct cagcgacagt
     2101 ccggccgggg tgagttccgc gatcatccgg caagtattgc ccacttcgat tacaacgtcg
     2161 gtatgcgaag gacacctacg acggcggctt tgatgacggc gcgtgcgctg gcgattgtgg
     2221 cggtactcgg acttgtggct gccgggtgcg gcggttccga ctcgtccagc gccaaccccg
     2281 gcggcgctga caacgctcag aacgccgtca cgaccacggc tggcaccggc gacgccgaca
     2341 ccgacggtgc cgaagctcgg gtggcggaat cgcccggctg tgcggcggcc gcccccgaaa
     2401 cccccggacg cgaggtccgc atcgatctgg cgtcgggagg ggtcgaacgc ttcgcgctcg
     2461 tcaccacgcc gagcgccgcg gcggagtcgg tggagccgcg ggcgttggtc atcgatttcc
     2521 atgggctcgc cgagggtgct gaggtgcacg cccggatgag ccagtacagc gaacttgccg
     2581 aacaggaggg gttcttcgcc gtgttcccgc agggcaccgg cgacatcgcg tcgtggaagg
     2641 cgggcgtcgg tggagaactc aaccccgacc acgcggatgt cgagttcgtc accgatctgc
     2701 tcgagcgact cggtgccgat cactgcatcg atctcggttc ggtctacgcc acggggttga
     2761 gcaacggggc gatgatgacc tcgacggtgg ggtgcctgct cgccgatcga ttcgcggcgg
     2821 tggcaccggt cgccggcgcg gcgttctata acgactgccc gtcgaccacg ccgatgccgg
     2881 tctacgcgat ccacggcacc caggatccgc tgctgccgtt caacggaggg gtcggaaacg
     2941 tcggtgcggt gatcgccggc ggagcggccg accagaccaa ggtggaggtc gagctctggc
     3001 acgatggcgt gcctcgcaaa actggagcta cgcgccggat caccgatgat cccgcccata
     3061 aagctccgaa aggaacgtac cagcaatatg tccacctatg atgtctccag aatcgatgaa
     3121 ctcgcccgcg atagcatgca tggcg
//
LOCUS       DAOOMA010000294         3142 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_37576_length_3142_cov_3.252672, whole genome shotgun sequence.
ACCESSION   DAOOMA010000294 DAOOMA010000000
VERSION     DAOOMA010000294.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 3142)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3142)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3142
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37576_length_3142_cov_3.252672"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..669
                     /locus_tag="PLP95_08445"
     CDS             <1..669
                     /locus_tag="PLP95_08445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HPB45865.1"
                     /translation="AGGTAEVDPALAELLRSLVVNGVFRDTGGMTSLRIFSSRKPVIG
                     AINGAAVGIGVTMTLPMDIRLVSTKAKLGFVFNARGIAPEAASTYFLPKIVGISQAAE
                     WMFTGRIFGAAEALAGGLVRSVHEPDELLAAAYSLAAEIRDNTAPVSTTLTRAMLWQL
                     LGASHPMAAHRIDSRAVQYTGTLPDALEGVMSFFERRSPQWQGKVSADLPDWYPFWDE
                     PDFE"
     gene            742..2691
                     /locus_tag="PLP95_08450"
     CDS             742..2691
                     /locus_tag="PLP95_08450"
                     /inference="COORDINATES: protein motif:HMM:NF021489.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2207 domain-containing protein"
                     /protein_id="HPB45866.1"
                     /translation="MAGAVAAIVVAFIAVLGVGAAAAGASGANDLWATVGRGPMRQGG
                     DVEQIESFRSEITLNDDGSIDVVETIVYDFGPNSRHGIQRYIPTRGRWTGEVPEGEKP
                     GTWWRLTPISKLEVTGSGDAPDDVEQMTSSDGIDTILRIGDPDRYVDGVRTYTIRYHM
                     AGVMNTFEHTDEFAYNITGNGWGVPIESTTAKLSLPGSPSRNWCFAGPYESTAPCSEI
                     AVSGSTVSASENLLRARSGLTVAVEFPSGVVARPMASAKFERERTLADSFEVTPATGV
                     TAAVGLLGGLAGLGFLGYRHGRDRHFVGDQIDYVFGNDSGEETPRKLFSKHTPTVEFV
                     PPDNIRPGHMGTLWDEVAHHLDVSAMLVDLAVRGWIRIDEIAPRSKGFLGFGGDSGDF
                     QLVSLKSANDKELLPAEKLLLDSLFSGGSDSVLLSELKTRFAEKMKLIQSRLYDDVVA
                     AGWFPRRPDKVRESYVGLGVLVLIAGVVLTFLAAKVSNWALPVLAIPVVGLVLVAAAK
                     LFPRRTARGSAMLSRVRGFKELFDAGEGERMAYAEQHNVFAQYLPYAIVFGATERWAK
                     TFQDLGLSPEEMGLGVWYTSPYGYDPIHFAWAMNSFTTHTTGALAAAAPSSASSGSGS
                     WSGGSFGGGGGFSGGGFGGGGGSSW"
     gene            2792..3142
                     /locus_tag="PLP95_08455"
     CDS             2792..3142
                     /locus_tag="PLP95_08455"
                     /inference="COORDINATES: protein motif:HMM:NF019845.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HPB45867.1"
                     /translation="MAQTVRGVIARAKGEPVSIEEIVVPDPGPGEVVVRVQACGVCHT
                     DLHYREGGINDEFPFLLGHEAAGIVEEVGEGVSNVAVGDFVVLNWRAVCGECRACKKG
                     LPHLCFAAHNTPRR"
BASE COUNT          513 a          949 c         1109 g          571 t
ORIGIN      
        1 gcgggtggaa ccgcggaggt cgaccccgcg ttggccgagt tgctgcgttc gctcgtggtc
       61 aacggggtct ttcgcgacac gggcggcatg acgagtctgc gcatcttctc gtcgcgaaag
      121 ccggtgatcg gcgcgatcaa cggcgcggcg gtgggcatcg gcgtgacgat gacgttgccg
      181 atggacatcc gcctggtgtc gaccaaggcg aaactcgggt tcgtgttcaa cgcccgcggc
      241 atcgccccag aggccgcctc gacgtacttt ctgcccaaga tcgtggggat cagccaggcg
      301 gcggaatgga tgttcaccgg gcggatcttc ggagcggccg aggcgcttgc gggcggcctg
      361 gtccgctcgg ttcacgaacc cgacgagttg ctggcggccg cctattcgct tgccgcggag
      421 atccgcgaca acaccgcccc ggtgtcgacg acgttgaccc gagccatgtt gtggcagctc
      481 cttggcgcat cgcatccgat ggctgcgcat cgcatcgaca gccgcgcggt ccaatacacc
      541 ggcaccctgc cggacgcgtt ggagggggtc atgagcttct tcgaacgtcg atcgccgcag
      601 tggcagggca aggtgagcgc ggatctgccc gactggtatc cgttctggga cgaaccagac
      661 ttcgaatgac gcgccggtaa cctctcggca atgaggtcgg caatgaggtc gtcggtgtgt
      721 ccgtctcgag atcgcggtcg cgtggccggt gcagtggcgg cgatcgtcgt cgcgttcatc
      781 gccgttctcg gcgtgggggc ggctgcagcc ggcgcatccg gtgcgaatga cctttgggcg
      841 acggtcgggc gcgggccgat gcgccaaggc ggcgacgtcg aacagatcga gtcgttccga
      901 tccgagatca ccttgaacga cgacggctcg atcgacgtcg tcgagaccat cgtctacgac
      961 ttcggcccga attcgcgtca cggcattcag cgctacatcc cgacgcgtgg acgctggacc
     1021 ggcgaggttc ccgagggcga gaagccgggc acctggtggc ggttgacgcc gatttcgaag
     1081 ttggaggtaa cggggagcgg cgatgccccc gacgatgtcg aacagatgac gtcgtctgat
     1141 ggcatcgaca cgatcctgcg tatcggggat ccggatcggt acgtcgacgg ggtgcggacc
     1201 tacaccatcc gctaccacat ggccggagtg atgaacacct tcgagcacac cgacgaattc
     1261 gcctacaaca tcaccggcaa cggctggggc gttcccatcg agtcgacaac cgcgaagctg
     1321 tcgcttccgg gttcaccatc gcgcaactgg tgtttcgctg ggccctatga gtcgacggcg
     1381 ccgtgcagcg agatcgcggt cagcggttcg acggtgtcgg ccagcgagaa tctcctgcgg
     1441 gcacgaagcg ggctgacggt cgccgtcgag ttccccagcg gcgtcgtcgc acggccgatg
     1501 gcctcggcga aattcgaacg cgagcgcacc ctcgcggatt ccttcgaggt gaccccggcg
     1561 accggagtga ccgcggcggt cggcctgttg ggcgggctcg cagggctggg gtttctcggc
     1621 taccgccatg gccgcgaccg tcactttgtc ggcgaccaga tcgactatgt gttcggcaac
     1681 gacagcggcg aagaaacccc ccgcaaactg ttctcgaagc acaccccgac agtggagttc
     1741 gtaccgccgg acaacattcg cccgggccac atgggcaccc tgtgggatga ggtcgcccat
     1801 cacctcgacg tgagtgcgat gctggtcgat ctggcggtcc gcgggtggat acggatcgat
     1861 gagatcgcgc cccggtcgaa ggggttcctc ggctttgggg gcgacagcgg cgacttccag
     1921 ctggtgtcgc tgaagtcagc caacgacaag gagttgttgc cggccgagaa gctgctgctc
     1981 gacagcctct tttctggcgg gtcggactcg gtgttgttga gcgaactgaa gacccggttc
     2041 gccgagaaaa tgaagttgat tcagtcgcgg ctctacgacg acgtggtcgc cgccgggtgg
     2101 tttccgaggc gtcccgacaa ggtgcgcgaa agctacgtgg gcctcggggt tctggtgttg
     2161 attgccggag tcgtgttgac cttcctcgcc gcgaaggtgt cgaactgggc gctgccggtg
     2221 ctggcaattc cggtggtcgg tctggtgctg gtcgccgccg ccaagctttt cccgcgccgc
     2281 actgcgaggg gctcggcgat gttgagccgg gttcgagggt tcaaagaact gttcgacgcc
     2341 ggcgagggtg aacggatggc ctatgccgaa cagcacaacg tgttcgccca gtatctgccg
     2401 tacgcgatcg tgttcggagc gactgagcgt tgggcgaaga ccttccagga tctcggcctg
     2461 tcgcccgagg agatgggcct cggcgtgtgg tacacgagcc cgtacggata cgacccgatc
     2521 cacttcgcct gggcgatgaa cagtttcacc acccacacca ccggtgccct cgcggccgcg
     2581 gcgccctcgt cggccagttc cggttccgga tcgtggtccg gaggaagctt cggcggaggc
     2641 ggtggtttca gcggcggcgg attcggcggt ggcggcggct catcgtggtg atgggtgggc
     2701 ccgccaatcg gttcgctctg caagactcgc cacagtgcgc cccgtcctca cccgagacgg
     2761 cggtgcggat cgaccagtga ggggagcgac catggcacag accgtgcgtg gagtaattgc
     2821 gagggccaaa ggggaacccg tttcgatcga ggagatcgtc gtgcccgatc caggaccggg
     2881 cgaggtcgtg gtgagggtgc aggcgtgcgg ggtgtgccac accgacctgc attatcgcga
     2941 aggcggaatc aacgacgagt tcccgttcct gctcggtcac gaggcggccg gcatcgtcga
     3001 ggaggtgggt gagggcgttt ccaacgttgc ggtcggcgac ttcgtcgtgt tgaactggcg
     3061 tgcggtgtgc ggtgagtgcc gggcgtgcaa gaagggcctg ccccatctgt gtttcgccgc
     3121 ccacaacaca cccagaagat ga
//
LOCUS       DAOOMA010000295         3141 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_37585_length_3141_cov_4.156189, whole genome shotgun sequence.
ACCESSION   DAOOMA010000295 DAOOMA010000000
VERSION     DAOOMA010000295.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 3141)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3141)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3141
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37585_length_3141_cov_4.156189"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..428)
                     /locus_tag="PLP95_08460"
     CDS             complement(<1..428)
                     /locus_tag="PLP95_08460"
                     /inference="COORDINATES: protein motif:HMM:NF020252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HPB45868.1"
                     /translation="MRTSATPTPRTLLRPNAPGGLRPPDVSTAPSGSNAADGPTDVDP
                     PADVEALMARLDTISIAHPSAVRVLMLVDDPNVTIHKVGEAIETDPAFTAQIMRLANS
                     AYYGLSGRVGNINFAVTIIGFAAVRSLAAMAAAGVTASGV"
     gene            complement(425..2524)
                     /locus_tag="PLP95_08465"
     CDS             complement(425..2524)
                     /locus_tag="PLP95_08465"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HPB45869.1"
                     /translation="MQDPQGAAATTTVTPLRASAIDTALASGVAAAPDPAIVVMTTND
                     GVRIEWANRAFCDLLMADPADLNGQRLDGLMTLDWEWTLNPLRSTRSHARLTSQLGAV
                     SSWSLRSCPISDGDLTRWTLFFSADDEDATDEVATDAHRYRALTERAPIGIFASEAGL
                     RLGYVNDWLAELVEVPADQLLGVGWMSIFRSEDLEVVMGCMQQALDGVSSECPARFVT
                     ASGEQRSVSIRIVPHQSNSGPASFLGTVEDVTDRMRVEELLEWQASHDSLTGLKNRSG
                     LIDAITTTLLECPTSTSVVFIDLDNFKLVNDLLGHAAGDDLLKTAANALTSAVRSGDE
                     VFRFAGDEFVAILRNTNTDDEALRAAERIGLALGTGIESCATVTPVDGSVGVVRSTLE
                     STAESLLGQADAAMYTAKRRGKGTVVLFDESMDASSGFTSQRHELDTDAIERGEVTRG
                     YVPIFPVSSGAGDRSPEPHALSCRVTWTDPDGQVHRDEELWEAARESGMVPTLLFANI
                     GQACEDLSAFRRSGVDAPGGVVIALAVADLCVPGLADQIARSLVRCRLTGGDITLVLH
                     KYDATVPSMVNTTIDQVRDLGVKVWARWESVSLQRFAVGAHRPDGVVIDVSELDAGNH
                     AGYEALITMLRGSGTAMCAHRVDSADRLATVDRLGIEWGVGAALGSIDCLQAWPAPSK
                     PALDAGSSAPSNPDEER"
     gene            2707..>3141
                     /locus_tag="PLP95_08470"
     CDS             2707..>3141
                     /locus_tag="PLP95_08470"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HPB45870.1"
                     /translation="MLKRLATNYLRPYKRLIVFVLLFQLIATIANLTLPSLNAAIIDN
                     GVAKNDNGYIWRHGGIMLAVSLVQVVFSILAVYYGSRASMGLGRDVRHDLFHQVTGFS
                     SREVNELGAPSLITRITNDVTQVQTLVQMTCTMFIAAPIMAVG"
BASE COUNT          554 a         1052 c         1003 g          532 t
ORIGIN      
        1 ttcaccccac tcgccgtcac cccagccgca gccatcgcgg caagcgagcg aaccgccgcg
       61 aagccgatga tggtcaccgc gaagttgatg ttgccgacgc gccccgacag cccgtagtac
      121 gccgagttcg cgagcctcat gatctgtgcg gtgaacgccg ggtcggtctc gatcgcctcg
      181 ccgaccttgt ggatcgtgac gttcggatcg tccaccaaca tgagcacccg caccgcggac
      241 ggatgagcga tggagatcgt gtcgagacgg gccatgagcg cctcgacgtc ggcaggtggg
      301 tcgacgtcgg tcggtccatc cgcagcgttc gatccggatg gggcagttga gacatccggt
      361 gggcgcaacc ctcctggcgc gttcggccga aggagtgtcc gtggggtcgg ggtggcggag
      421 gtcctcatcg ttcctcgtct gggttggacg gtgccgacga gccggcgtcg agggcgggtt
      481 tcgagggagc cggccacgcc tgaagacagt cgatgcttcc gagcgcagcg ccgacgcccc
      541 actcgatccc gagccgatcg accgtggcca accgatccgc ggagtcgacc cggtgtgcac
      601 acatggccgt gcccgaacca cgcaacatgg tgatgagcgc ctcataaccg gcgtgattgc
      661 ccgcatcgag ttccgaaacg tcgatcacga cgccgtcggg ccggtgagct ccgaccgcga
      721 agcgttgtag ggacaccgac tcccagcgcg cccatacctt caccccaagg tcgcgcacct
      781 ggtcgatcgt ggtgttgacc atcgacggaa ccgtggcgtc gtacttgtgc agcaccaggg
      841 tgatgtcgcc gccggtcaaa cgacaacgca cgagcgaacg agcgatctga tcggccagcc
      901 cgggaacgca caggtcggcg acggccaacg cgatcacgac tccaccggga gcatcgacgc
      961 cgctgcgacg aaatgccgag aggtcttcgc acgcctggcc gatgttggcg aacaacagcg
     1021 tcggcaccat ccccgactcg cgggcggcct cccacaactc ctcgtcacgg tgcacctgcc
     1081 cgtcggggtc ggtccaggtc acacgacacg acagcgcgtg gggttccggc gaccggtcgc
     1141 cggctccgct gctgacagga aaaatcggca cgtacccgcg ggtgacctca cctcgttcga
     1201 tggcgtcggt gtcgagctcg tgtcgctgcg aggtaaaccc actcgacgca tccatggact
     1261 cgtcgaacag caccaccgtg cccttacccc ggcgctttgc ggtgtacatg gcggcatcgg
     1321 cctgcccgag caacgactct gcggtggatt cgagggtcga acgcacgacc cccaccgatc
     1381 cgtcgaccgg tgtgaccgtg gcacagcttt cgatcccggt gccgagggcg agcccgatac
     1441 gttcggccgc ccgcagggcc tcgtcgtcgg tgttggtgtt gcgcaggatt gcgacgaact
     1501 cgtcaccggc aaaccgaaac acctcgtcgc ccgagcgcac cgccgaggtc aacgcgttcg
     1561 ctgccgtctt caacagatcg tcgccggcgg cgtggccgag cagatcgttg acgagcttga
     1621 agttgtcgag gtcgatgaac accaccgacg tcgaggtcgg acattcgagc agcgtcgtgg
     1681 tgatcgcgtc gatcaggccc gaacggttct tcagcccggt gagcgagtcg tggctcgcct
     1741 gccactcgag gagctcctcg acgcgcatcc ggtcggtgac gtcctcgacg gtgccgagaa
     1801 acgacgccgg cccggagttg ctctggtggg gaacgatgcg gatcgacaca ctgcgctgtt
     1861 cgcccgacgc cgtgacgaag cgggccggac actccgacga gactccgtcg agagcctgtt
     1921 gcatacagcc catgacgacc tcgaggtcct cggaacgaaa gatcgacatc catccgacgc
     1981 cgagcagttg atccgcggga acctcgacga gttccgccaa ccaatcgttc acatagccga
     2041 ggcgaaggcc ggcctccgac gcgaagatcc cgatgggcgc ccgctcggtc agggcccgat
     2101 accgatgagc gtcggtggcg acctcatcgg tggcatcctc gtcgtcggcc gagaagaaca
     2161 gggtccatcg ggtgaggtcg ccgtcggaga tcgggcacga acgaagcgac cagctgctca
     2221 ccgctccgag ttgcgaggtc aggcgcgcat gggaccgggt cgacctcagc ggattgagcg
     2281 tccactccca atcgagggtc atcagtccgt cgaggcgttg accgttgagg tcggcggggt
     2341 cggccatcaa caggtcacaa aaggcgcggt tcgcccactc gatacgaacc ccgtcgttcg
     2401 tcgtcatgac cacgatcgcc gggtccgggg ctgcggcgac tccggaggcg agtgcggtgt
     2461 cgatcgccga agcacgaagc ggcgtcaccg tcgtcgtagc ggcagcacct tggggatctt
     2521 gcatggttct tgcatcggcg tgtgcacttc gaacttgagg tgcaaccgcc ccgcgctctg
     2581 gggtttttcc tccgacgggc gcgcgatcga gcaccgacga cggcattgaa cgcccgttcc
     2641 gctacaacgg cgtagtgttt cccgttgcgc tcggcgcagc catcccaacg accctccagg
     2701 atccacatgt tgaaacgtct ggcgacgaac tatctgcggc cctacaagcg gctcatcgtt
     2761 ttcgtcctgt tgttccagct catcgcaacg atcgccaacc tgacgttgcc gagcctcaat
     2821 gcggccatta tcgacaacgg cgtcgccaag aacgacaacg gctacatctg gcgccacggc
     2881 ggcatcatgt tggccgtctc gctcgtccag gtggtgtttt cgatcctcgc cgtctattac
     2941 ggttcgcgcg cctcgatggg cctcgggcgc gatgtccgcc acgacctgtt ccaccaggtg
     3001 accgggttct ccagccgtga ggtcaacgaa ctcggggcgc cgtcgttgat cacccgcatc
     3061 accaacgacg tcacccaggt ccagaccctc gtgcagatga cctgcacgat gttcatcgct
     3121 gcgccgatca tggccgtcgg c
//
LOCUS       DAOOMA010000296         3125 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_37821_length_3125_cov_3.580130, whole genome shotgun sequence.
ACCESSION   DAOOMA010000296 DAOOMA010000000
VERSION     DAOOMA010000296.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 3125)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3125)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3125
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37821_length_3125_cov_3.580130"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            189..1334
                     /locus_tag="PLP95_08475"
     CDS             189..1334
                     /locus_tag="PLP95_08475"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012229417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HPB45871.1"
                     /translation="MRLFDTAADAIVPFEVGPVVRMYTCGITPYDSTHLGHAATYLTY
                     DVLQRRLRDLGHETRCVRNITDVDDSILGKARELGVHYLDLAAAEIARFDNDMATLEM
                     IPSYAEPRATSAIADIRNFIGKVLDGGYAYQAGGSVYFEIAKFPKFGQISHYDRAHML
                     QCAAERGGNVEDLHKRDPLDFVLWHPSLPDEPSWDSLWGPGRPGWHIECSALCLREHG
                     NATIDLHGGGADLIFPHHECEAAQSEAATGQSFARYWMHQAMVRMDGEKMSKSLGNLV
                     FVSDLVKEWDPRSIRLAVVENHYRTEWEWDDTRMARNDERLRAWVAAGPGSGALDSVR
                     SALDEDLNTTEAVAAIDSAAAAGLGVSEAAVLLGVDTSRAVAAGVGG"
     gene            1431..2321
                     /locus_tag="PLP95_08480"
     CDS             1431..2321
                     /locus_tag="PLP95_08480"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HPB45872.1"
                     /translation="MDQQDAGRLQPLFRAVLTPGFRFLWRVRTEGLENIPEHGPAILA
                     PNHTSVLDSFFVPLVLKRRMTYVGKAEYLDDWKTKWLFPAMGMIPIDRSGGDASKGAL
                     DAAAGVLDRGELFGIYPEGTRSRSGKLHKGHTGVARLALRCKAPIIPVGIVGTVEVQP
                     PDAKTPKPFREVTIRFGKPIDVTRYCDRASDRMVLRQITDEVMYSIRDMSGQEYVDTY
                     ATKQAAGAAKPATPTPATTQPTPIIELDPVIEVNGPVLEPAVGSQAAIDATGSRSGAT
                     APKSSAEVLRQSRPLVDLTI"
     gene            2400..>3125
                     /locus_tag="PLP95_08485"
     CDS             2400..>3125
                     /locus_tag="PLP95_08485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012229421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TGS domain-containing protein"
                     /protein_id="HPB45873.1"
                     /translation="MSNITITLPDGSERELPEGATGTTLAGEIGPGLAKAALVVEVDG
                     TPVDLAAPLSDGVAVRFLTDRDPDVLEHLRHSTAHVLAQAVLELWPGATFAGGPPIAD
                     GFYYDFELPDGATFSDADLDAIDAKMREIIGADQPFERFELPLGEAKNLMADHPYKQA
                     FMDLAQSGDDSDGEVAGGETISFYRNTPEFVDMCRGPHVPSTGRLGHFKLMRVAGAYF
                     RGSEKNPMLQRIYGTAWANKKELA"
BASE COUNT          547 a         1077 c         1025 g          476 t
ORIGIN      
        1 cgggcacgat ggcggtgtcc gcgccggggt ccgagacgcc ggcgtcggcg agcggatccg
       61 ccaccgacgc aggcaccgac gcaggcaccg acgccggcac cgccgccggc accgccgcag
      121 ctggcgccga caccggtacc ggctcggccg aatagccgcc ccaggacact cgactagcgt
      181 tggcggccgt gcgactcttc gataccgccg ccgacgcgat cgtcccgttc gaggtgggcc
      241 ccgtcgttcg gatgtacacc tgcgggatca ccccgtacga ctcgacccac ctcggccatg
      301 cggcgaccta tctgacctac gacgtgttgc agcgccggtt gcgcgacctc ggccacgaga
      361 cccgctgcgt tcgcaacatc accgacgtcg acgactcgat cttgggcaag gcccgcgaac
      421 tcggggtgca ttacctcgac ctggcggccg cagaaatcgc ccgcttcgac aacgacatgg
      481 cgacgttgga gatgatcccg tcgtatgccg agccccgcgc cacctcggcg atcgccgaca
      541 tccgcaactt catcggcaag gtgctcgacg gcggatacgc gtaccaggcc ggcggatcgg
      601 tgtatttcga gatcgccaag ttcccgaagt tcggccagat cagtcactac gaccgcgccc
      661 acatgttgca gtgtgcggcc gaacgcggcg gcaacgtcga ggacctccac aagcgggatc
      721 ccctcgattt cgtcctgtgg cacccgtcgc tgcccgatga gccaagttgg gattcgctgt
      781 ggggtccggg gcgtccgggt tggcacatcg agtgctcggc gctgtgcctt cgcgaacacg
      841 gcaacgccac gatcgacctt cacggcgggg gagcggacct gatctttccg caccacgagt
      901 gtgaggcagc gcaatcggag gccgccaccg gccagagctt cgcccgctac tggatgcacc
      961 aggcgatggt gcgcatggac ggcgagaaga tgtcgaaatc cctcggcaac ctcgtgttcg
     1021 tgagcgacct ggtcaaggag tgggacccgc gctcgattcg cctcgcggtg gtcgagaacc
     1081 attaccgcac cgaatgggag tgggacgaca cccgaatggc ccgcaacgac gagcggctgc
     1141 gggcctgggt ggcggcgggc cccggcagcg gggcgctcga ctcggtgcgt tccgcactcg
     1201 acgaggacct gaacaccacc gaggccgtcg cagcgatcga ttccgccgca gcggcggggc
     1261 tcggggtcag cgaagctgcg gtactcctcg gtgtcgatac cagtcgagcg gtggcggccg
     1321 gcgtcggtgg ctgaagaatt tcatcggcag ttcatcgaat tgatccaccc gacccgtcaa
     1381 ttcgcgccat catgggccga cgacattccc agggactgaa gggaggggtg atggatcaac
     1441 aggatgcggg acgactacaa ccactctttc gggcggtgct caccccggga ttccgattcc
     1501 tctggcgggt gcgcaccgag gggctcgaga acatcccgga acacggaccg gcgattctcg
     1561 ccccgaacca cacctcggtg ctcgacagct tcttcgtgcc gctcgtgctg aagcggcgca
     1621 tgacctatgt cggcaaggcg gaatacctcg acgactggaa gaccaagtgg ctcttcccgg
     1681 cgatggggat gatcccgatc gatcgttccg gcggcgatgc ctccaagggt gcgctcgatg
     1741 ccgcagccgg tgtgctcgat cgcggcgaac tgttcggcat ctaccccgag ggcacccgca
     1801 gccgcagcgg caaattgcac aagggccaca ccggagtcgc ccgcctggcg ttgcgctgca
     1861 aggcgccgat cattccggtc ggcatcgtcg gaaccgtcga ggttcagccc ccggacgcca
     1921 agacccccaa gccgtttcgc gaagtgacga tccggttcgg caagccgatc gacgtcaccc
     1981 ggtactgcga ccgggcgagt gaccgcatgg tgctgcgcca gatcaccgac gaggtgatgt
     2041 attccatccg cgacatgtcg ggacaggagt acgtcgacac ctacgccacc aagcaagcgg
     2101 ccggggcggc gaagccggcg accccgacgc ctgcgaccac gcaaccgacc ccgattatcg
     2161 agctcgaccc ggtcatcgag gtgaacggcc ccgtcctcga acccgccgtc ggtagccaag
     2221 ccgccatcga cgccacgggc agccgctcgg gggccacggc gccgaagtcg tcggccgagg
     2281 tgcttcgcca gagccggcca ctcgtcgatc tgacgatctg acgcccacgg ccgcacccgt
     2341 cgggaacccc ggccgttact ccaccgggag ggtggttggc cgccggtagg attggcgcca
     2401 tgtcgaacat cacgatcacc ctgccagacg gatccgagcg cgaactcccc gagggcgcca
     2461 ccggaaccac gctggccgga gagatcggtc cgggcctcgc caaggcggcc ctcgtggtcg
     2521 aggtcgacgg cacccccgtc gacctcgcgg caccgttgag cgacggggtc gcggtgcggt
     2581 ttctcaccga tcgcgacccc gacgtgctcg aacacctccg ccactcgacc gcgcatgtgt
     2641 tggcccaggc cgtgctcgag ctgtggccgg gtgcgacctt cgccggtggg ccgcccatcg
     2701 cagacggctt ctactacgac ttcgagttgc cggacggcgc aaccttttcc gacgcggacc
     2761 tcgatgcgat cgacgcgaag atgcgcgaga tcatcggtgc cgaccaaccg ttcgaacgct
     2821 tcgagttgcc gctcggcgag gcgaagaacc tcatggcgga tcatccctac aagcaggcgt
     2881 tcatggatct cgcccagtcg ggcgacgact ccgatgggga ggtcgccggc ggtgagacga
     2941 tcagcttcta tcgcaacacg ccggaattcg tcgacatgtg ccgcggcccc cacgtgccgt
     3001 cgacggggcg gctcggccac ttcaagttga tgcgggtcgc cggcgcctat ttccgcgggt
     3061 cggaaaagaa cccgatgctg cagcgcatct acggcaccgc gtgggcgaac aagaaggaac
     3121 tggcc
//
LOCUS       DAOOMA010000297         3124 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_37837_length_3124_cov_3.863799, whole genome shotgun sequence.
ACCESSION   DAOOMA010000297 DAOOMA010000000
VERSION     DAOOMA010000297.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 3124)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3124)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3124
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37837_length_3124_cov_3.863799"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1005
                     /locus_tag="PLP95_08490"
     CDS             <1..1005
                     /locus_tag="PLP95_08490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB45874.1"
                     /translation="RSVVVALVCAVAVGAIALSVVGSWVNSIIDSPARTSDAVAAALN
                     DEEVVSDLATAAADRAVSAAMEVLGIDDADSTSNPVVRRLRSTAQEWAADVAAARIAA
                     PVIGSDIANSLATSAAGRLHRVIVETVVDGGGLGEISADEPFRVNLVPLVVDALAAAQ
                     TVGVLPSVELPDLAQIDGADAQRAAVSEALGVTLPRNFAEYTIAREGSVGGVLAVIEF
                     VVSLAQRVGLYAPLIAVGATVSALALARRRLRTLGALAAATAVMMVLLWGSTQAGIDR
                     LDDAGLTPGAVRAIRIVAEELAAPLATAALWVGAASLAIAVVATAARGRGKRGVTHAT
                     "
     gene            995..1372
                     /locus_tag="PLP95_08495"
     CDS             995..1372
                     /locus_tag="PLP95_08495"
                     /inference="COORDINATES: protein motif:HMM:NF016246.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 family protein"
                     /protein_id="HPB45875.1"
                     /translation="MRRDLKDHVHIERAYDPPEPGRRRVLVDRLWPRGVSKERAALDE
                     WCKDVAPSTELRKWYDHAVDRFEEFSSRYQDELRHGDAGAAVERLRTAAQTGELALVT
                     ATRDIEHSGAIVLREFLLGGAGI"
     gene            1440..2291
                     /locus_tag="PLP95_08500"
     CDS             1440..2291
                     /locus_tag="PLP95_08500"
                     /inference="COORDINATES: protein motif:HMM:NF012472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HPB45876.1"
                     /translation="MNNPQVTLNDGVTIPTLGFGVYQVVPEETERVVTDALEVGYRHI
                     DTAAAYGNEEGVGRAIARSGIARDKLFVTTKLWIVDGGEQGARRAFESSLERLGLGYV
                     DLYLIHQPYGDYYSSWRAMEALQREGLVRSIGVSNFHPDRLIDLIDHNEVVPSVNQVE
                     LNPYFQRADEQRWMADHGVRIESWGPFAEGKNNLFEDPTLSSIGAGYDKSVAQVVTRW
                     LIQREVLVIPKSVRRQRMEENFDVFDFDLTAGEMARIAELDTGVSQFFDHRDPKMAHV
                     LGGVRVD"
     gene            2372..3010
                     /locus_tag="PLP95_08505"
     CDS             2372..3010
                     /locus_tag="PLP95_08505"
                     /inference="COORDINATES: protein motif:HMM:NF025255.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4203 domain-containing protein"
                     /protein_id="HPB45877.1"
                     /translation="MADIALGLVAIIAGAAFCFAGYQWFRLIIPVWGFFAGFSAGTGL
                     ISAITGDAVLATPLGWILGLVLAVVFATFAYLYYSVAIVMAMSSIGFMLGGALMTALG
                     VPWNWAIAIVGILVGVAFAFAGIVSNLPRVVLVVVSAIGGATAIVGGLMLFGSTLDTE
                     DFSRAVITETINDSWGWWLLYAVLTIAGIMTQSAIASTEDELRAAWMSVDQN"
BASE COUNT          483 a          936 c         1130 g          575 t
ORIGIN      
        1 cgctcggtcg tggttgccct ggtctgcgct gtcgcggtgg gagcgatcgc gttgtcggtg
       61 gtgggttcgt gggtgaactc gatcatcgat tcgccggcgc gcaccagcga cgcggtggca
      121 gcggcactaa acgatgagga ggtcgtgtcg gacctggcga ccgctgcggc cgaccgggcg
      181 gtgagcgcgg cgatggaggt gttgggaatc gacgacgcgg actcgacgtc gaatccggtg
      241 gttcggcggc tccgctcgac cgcacaggag tgggctgccg atgtggcggc tgcgcgaata
      301 gcggcgcccg tgatcgggtc cgatatcgcc aactccctcg caacgtctgc ggcaggtcgg
      361 ctccatcggg tcatcgtcga gacggtcgtc gacggcggcg gcctcgggga gatctccgcc
      421 gatgagccgt ttcgggtcaa cctcgtgccg ttggtggtcg acgcactcgc agcggcgcag
      481 acggtcggag tgctgccgtc ggtcgaactg cccgatctcg ctcagatcga cggggcggac
      541 gcgcagcgtg cagcggtgtc cgaagcgctc ggggtgacgc tgccgagaaa tttcgccgag
      601 tacacgatcg cccgggaggg gtcggtcggt ggagtccttg cggtcatcga gttcgtggtc
      661 tcccttgccc agcgtgtcgg gctctatgct ccgctgatcg cggtgggggc gacggtgtcg
      721 gcgttggcgc ttgcccggcg acggttgaga acactcggcg ctctggctgc cgccaccgcg
      781 gtgatgatgg tgctgctgtg ggggtcgacc caggccggga tcgaccggtt ggacgatgcc
      841 ggcctcaccc ccggggcggt ccgggcgata cgcatcgtgg cggaggaact cgcggccccg
      901 ctcgctacag ccgccttgtg ggtcggcgcc gcgagccttg cgatagccgt ggttgccacc
      961 gcggcacgag gccgggggaa gaggggggtg acccatgcga cgtgacctca aggaccatgt
     1021 gcacatcgag cgcgcttatg acccacccga gcccggccgt cggcgggtgc tcgtcgaccg
     1081 cctgtggccc cggggcgtct ccaaggaacg ggccgccctc gacgagtggt gcaaggacgt
     1141 ggcgccgagc accgagttgc ggaagtggta cgaccacgcg gtcgaccgat tcgaggagtt
     1201 ctcctcccga taccaggacg aactgcgcca cggcgatgcc ggagctgcgg tcgagcgcct
     1261 cagaaccgcc gcccaaaccg gggagttggc gttggtcact gcgacccgcg atatcgagca
     1321 ctccggggcc atcgtcctgc gggaatttct tcttggcggg gccggtatct gaatcgaaac
     1381 cgggcgtcac ggcgcaagcg gcgagttgta gcgtgagctc gagacgagga ggggcgcaga
     1441 tgaacaaccc gcaggtcacg ctgaatgacg gcgttacgat tccgaccctg ggattcggcg
     1501 tctatcaggt cgtcccggag gagaccgaac gcgtcgtcac cgatgcgctc gaggtcggct
     1561 atcgccacat cgacaccgct gccgcctacg gcaacgagga gggggtcggc cgagcgatcg
     1621 cgcgcagcgg tatcgcccgc gacaagctgt tcgtgacgac gaaactgtgg atcgtcgacg
     1681 gaggcgaaca gggcgcgcgg cgcgcgtttg agtcgtcgct cgaacgtctc ggcttgggct
     1741 atgtcgatct gtatctgatc catcagccct acggcgacta ctacagctcg tggcgggcga
     1801 tggaagcgct gcagcgagag gggctcgtcc gctcgatcgg ggtgtcgaac tttcacccgg
     1861 atcgcctcat cgatctcatc gatcacaacg aggtcgtgcc gtcggtcaac caggtcgagt
     1921 tgaacccgta tttccaacgg gccgacgaac agcggtggat ggccgatcac ggcgtccgga
     1981 tcgaatcgtg ggggccgttc gccgaaggca agaacaacct cttcgaagac ccgacccttt
     2041 cctcgatcgg tgcgggctac gacaagtcgg tggctcaggt ggtcacccga tggctcatcc
     2101 aacgcgaggt actcgtgatc ccgaaatcgg ttcggcgaca gcggatggag gagaacttcg
     2161 acgtgttcga cttcgatttg accgctggcg agatggcgcg gatcgctgag ctcgacacgg
     2221 gcgtgtcgca gttcttcgat caccgtgacc cgaagatggc ccacgtgctc ggcggggtga
     2281 gggtggattg atcgcttggc cgatccgtct aagcggctgc ccggcgaggt agcgtttggc
     2341 gggtacgggc caagcgaacg gagaacgggt catggcagat atcgcgttgg gcttggtggc
     2401 catcatcgcc ggggcggcct tttgtttcgc cggctatcag tggttccgac tcatcatccc
     2461 cgtgtggggg ttcttcgccg ggttcagcgc gggcaccggg ctcatctccg ccatcaccgg
     2521 cgatgcggtg ttggcgacac cgctggggtg gattctgggg ctcgtgctcg cggtcgtctt
     2581 cgcgacgttc gcatacctgt actactccgt ggcgatcgtg atggcgatga gctcgatcgg
     2641 gttcatgttg ggtggagcgc tcatgaccgc actgggggtc ccctggaact gggcaatcgc
     2701 catcgtcggc atcttggtcg gcgtcgcatt cgcctttgcc ggcatcgtgt cgaacctgcc
     2761 ccgagtcgtg ctggtggtgg tcagtgcgat cggcggagcg acggcgattg tcggcggcct
     2821 catgttgttc gggtcgaccc tcgacaccga ggacttctcg cgggccgtga tcaccgagac
     2881 gatcaacgac tcgtggggct ggtggctgtt gtacgcggtg ttgacgattg ccggaatcat
     2941 gacgcagagc gctatcgcct caaccgagga cgaactgcgc gccgcgtgga tgtcggtgga
     3001 ccagaactaa ccgaggtcgg ccggcgcctg tacgtcggcg gtcgggaagc gcgaacctga
     3061 cagctcctcg gagatcgtcc acaacctgtg ggcgagttcg cggtcgtggg tgcggccact
     3121 cgat
//
LOCUS       DAOOMA010000298         3095 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_38301_length_3095_cov_3.707895, whole genome shotgun sequence.
ACCESSION   DAOOMA010000298 DAOOMA010000000
VERSION     DAOOMA010000298.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 3095)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3095)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3095
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38301_length_3095_cov_3.707895"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..403
                     /locus_tag="PLP95_08510"
     CDS             <1..403
                     /locus_tag="PLP95_08510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015439868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HPB45878.1"
                     /translation="AHLAAAHMATNEPEDEERGVIISTASIAAYEGQIGQVAYTAAKA
                     GIAGMVLTMARDLGGLGIRALAIAPSLFSTGLTEGIPDEFAAMLTKDAAFPKRMGRPE
                     EYGKLVVAIAENPMLNGLCIRLNGGQRFAPK"
     gene            449..1330
                     /locus_tag="PLP95_08515"
     CDS             449..1330
                     /locus_tag="PLP95_08515"
                     /inference="COORDINATES: protein motif:HMM:NF012596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HPB45879.1"
                     /translation="MTSEHTLVERDGGLVTVTLNRPEKKNAINSPIWMDLDEILREVE
                     SNPSDRALLLAGAGGNFSSGADLSGGLSAKPEPGSPEALAAEERIRAAEAAGERVDKG
                     GLTGKGRQLIIHDMRLVGDIIQRLQRLPKPTIAAVDGVAIGVALGLAVACDLVVASER
                     ATFREVFVQRGLALDGGASWTLPRLIGLRRAKQMTFFGDAVDAATALEWGLVNEVVAP
                     DDLMATATEWAQRLANGPTTALSIIKRLLESGLDSSIADALEDEARAQHIVFGTHDFR
                     EGMTAYFERRPPNFIGE"
     gene            1429..2010
                     /locus_tag="PLP95_08520"
     CDS             1429..2010
                     /locus_tag="PLP95_08520"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HPB45880.1"
                     /translation="MSSTRRIGAEDSESRAALLDAAERLILTDGYAAVTSRKVAAEAG
                     LKPQLVHYYFRTMDELFVELFRRRAEDGLVRQAQVLESPRPLRALWEFSNQPTGSAFA
                     MEFAALANHRKDIRAEIGRFAEALRAQQLEIMTEVFERYAVDVTSYPAVTLLVLMTSV
                     SRLLVMEERLGMSTGHAETRALVERLIDQFEPS"
     gene            2132..>3095
                     /locus_tag="PLP95_08525"
     CDS             2132..>3095
                     /locus_tag="PLP95_08525"
                     /inference="COORDINATES: protein motif:HMM:NF025620.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate-binding domain-containing protein"
                     /protein_id="HPB45881.1"
                     /translation="MYRISTHIRRSAAALGAAALMAATGCSNDEAVESNQTSSTNPAN
                     SDSTTDSTTATTATIVDAAGDVDWTTLPSTDVTLSDDGLAITDAGTYVLTGSSTGQVT
                     VNAAGAVRIVLDGVNIESTAGAAIQVDQADLTVIELADDSDNSISDAATRRDEDIDAA
                     IYSSDDLVLTGNGMLNVTANFADGIVGQGDLAITSGTYALTTVDDGIRGTDSLTIDGG
                     TIDIVESVEGIEAPVIVIDDGDITINASDDGINASASDIITTGLSITINGGRITIVMA
                     SGDTDAIDSNGDLTITGGTIDITAQSAFDFDGTGTLTGGTITVNG"
BASE COUNT          564 a         1053 c          986 g          492 t
ORIGIN      
        1 cgcccacctc gccgccgccc acatggccac gaatgagccg gaggacgagg agcgcggcgt
       61 catcatctcg acggcctcga tcgccgccta cgaaggtcag atcggccagg ttgcgtacac
      121 cgcggccaag gcgggcatcg ccggcatggt cctcacgatg gcccgcgacc tcggcggcct
      181 cggcatccgc gccctcgcca tcgcgccgag cctgttctcc accggcctga ccgagggcat
      241 tccggacgag ttcgccgcca tgctcaccaa ggacgccgcc ttccccaagc gcatgggtcg
      301 ccccgaggag tacggcaagc tcgtcgtcgc catcgccgag aacccgatgt tgaacggtct
      361 gtgcatccgc ctcaacggcg gccaacgctt cgccccgaag tgatcggccg acgcatccaa
      421 ccgttcgcca ccacccgaaa agagtgtcgt gaccagtgag cacaccctgg tggagcgtga
      481 cggtggcctg gtcaccgtca ccctcaaccg ccccgagaag aagaacgcca tcaactcgcc
      541 gatctggatg gatctcgacg agatcctccg ggaggtcgaa tcgaacccgt ccgatcgtgc
      601 actgttgctc gccggcgcgg gcggcaattt ctcctccggt gcagacctga gcggaggcct
      661 gtccgccaaa cccgagccgg gttcgcccga ggcgctcgcc gccgaggaga ggattcgcgc
      721 ggcggaggcc gccggcgagc gggtcgacaa gggtgggctc accggcaagg ggcgccagct
      781 catcattcac gacatgcgtc tcgtcggcga catcatccag cggctccaac ggttgccgaa
      841 gccgaccatt gccgcggtcg acggggtggc catcggcgtg gccctcgggc tggcggtcgc
      901 ctgcgatctc gtggtggcgt cggagcgtgc gacatttcgt gaggtgttcg tccagcgcgg
      961 actggccctc gacggcggcg cgtcgtggac cctgccgcga ctcatcggcc ttcgccgtgc
     1021 caagcagatg accttcttcg gcgatgcggt cgatgcggca acggcgctcg aatggggcct
     1081 cgtcaacgag gtggtggcgc ccgacgatct gatggcgact gcgaccgagt gggcgcagcg
     1141 tttggcgaac ggaccgacga ccgcgctgag catcatcaaa cgcctcctcg agtccggcct
     1201 cgattccagc atcgccgatg cgctggagga cgaggcccgc gcccagcaca tcgtgttcgg
     1261 cacccacgac tttcgcgagg gcatgacggc gtacttcgag cgacgtcccc cgaacttcat
     1321 cggcgagtga cggccgcgct gcggtaggtt cctcgctggc cgagcaggcc ggaccaggtt
     1381 ggtccgggtt ggtcctctcg aggtggactg atcgacttgg ggagggatat gtcatcgacg
     1441 cgcaggatcg gagccgagga ttccgagagt cgcgccgccc tgctcgatgc cgccgagcgg
     1501 ttgatcctca ccgacgggta cgcggcggtg acctcacgaa aggtcgctgc tgaggcgggg
     1561 ttgaagcctc agctcgtcca ctactacttc cgcacgatgg acgagctgtt cgtggagctg
     1621 tttcgtcgac gggccgagga cgggctcgtt cgccaggcgc aggtgctcga atcgccgcga
     1681 ccgctgcgag cgctgtggga gttcagcaac cagcccaccg gatcggcctt tgccatggag
     1741 ttcgccgccc tggcgaatca ccgcaaggac atccgggccg agatcggtcg attcgccgag
     1801 gcgttgcgcg cacagcaact cgagatcatg accgaggtgt tcgagcgata cgcggtcgac
     1861 gtcacgtcgt acccagcggt cacgttgttg gtgctgatga ccagcgtgag tcggctgctc
     1921 gtcatggaag aacgcctggg catgtcgacc ggccacgccg aaacccgcgc cctcgtcgaa
     1981 cgactcatcg accagttcga gccctcatag cccgagggtg gcgaatcgcg attcccaggg
     2041 aatccacagg gatctctttc ggcgcacgga ggttggctac gtagtttcga cgacacagca
     2101 tcagtacttc cctgcgaata aggaccccgc gatgtaccgg atttcgacac acatacgacg
     2161 aagcgctgca gcactgggtg cggccgcact catggctgcg accgggtgct cgaatgacga
     2221 ggctgtcgag tcgaaccaaa cgagttccac gaaccccgcg aactccgact cgaccaccga
     2281 ctcgaccaca gccacgacgg ccaccatcgt cgatgcggct ggcgacgtcg attggaccac
     2341 gctgccgtcg acggacgtca ccttgagcga cgacggcctg gcgatcaccg acgccggaac
     2401 ctacgtcctc accggctcct ccaccgggca ggtcactgtg aacgccgccg gagccgtgcg
     2461 catcgtgctc gacggcgtga acatcgagag caccgcgggt gcggcgatcc aggtcgacca
     2521 ggccgacctc accgtcatcg agttggccga cgactccgac aactccatct ccgatgcggc
     2581 gacgcgtcgc gacgaggaca tcgatgcagc gatctactcc tccgatgacc tggtgctcac
     2641 cggcaacggg atgctgaatg tcacggcgaa cttcgccgac ggcatcgtcg gacagggtga
     2701 cctggcgatt acctcgggca cctatgccct caccaccgtc gacgatggca tccgaggcac
     2761 ggattcgctc acgatcgacg gcggcacgat cgacatcgtc gaatccgtcg aggggatcga
     2821 agcaccggtg atcgtgatcg atgacggcga catcaccatc aacgcctcag acgacggcat
     2881 caacgcctcc gcatcggaca tcatcacgac cggcctgagc atcaccatca acggcggtcg
     2941 aatcaccatc gtgatggctt ccggcgacac cgatgcgatc gacagcaacg gcgatctcac
     3001 gatcaccggc ggcacgatcg atatcaccgc ccagtccgcg ttcgacttcg acggcacggg
     3061 gacactgacc gggggcacga tcaccgtcaa cggcg
//
LOCUS       DAOOMA010000299         3093 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_38332_length_3093_cov_4.592166, whole genome shotgun sequence.
ACCESSION   DAOOMA010000299 DAOOMA010000000
VERSION     DAOOMA010000299.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 3093)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3093)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3093
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38332_length_3093_cov_4.592166"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            136..1512
                     /locus_tag="PLP95_08530"
     CDS             136..1512
                     /locus_tag="PLP95_08530"
                     /inference="COORDINATES: protein motif:HMM:NF013279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HPB45882.1"
                     /translation="MSVPTSGGAPARIRTPRSVEAGLLVLAGLIVAAAFVLVSLGRSR
                     ALPADLVPLLVGVFVLFAAAHIGVRRLAPRADALLLPSAVLLNGLGYVFIMRLDPDLG
                     AQQAGWTAVGVVGFIGVLAVIRRVRTLEGLRFTVGLVGVILLILPLLPGLGVTIYGAR
                     IWVRAGPISFQPGEFAKLALAIFFAGYLVERRELLGTATFKVGPFMIPEPKHLVPVVL
                     AWGTSLVVMVFERDLGSALLFFALFMSVLWIASGRISYVAIGSMLFAVGAVFAWNSFG
                     HVRTRVDVWLNPWDDVLDSGYQIAQATYAFAWGGVTGVGFGQGFGNRVPVAESDFIFA
                     IMGEELGLLGTTAVIVLFLTIIGSGFRIAMRSTNPFNKLLAVGLTTLIGFQSFIIMAG
                     VTRLLPLTGLTLPFVSYGGSSLVSNWMLIALLLRLSDEAETREAQRVAEDDRAADLGL
                     TTTIGVGV"
     gene            1524..3074
                     /locus_tag="PLP95_08535"
     CDS             1524..3074
                     /locus_tag="PLP95_08535"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HPB45883.1"
                     /translation="MNRQIVKLVAGLMVCYVALFARLNQIQVFDAKKLNKREDNTREM
                     IRDFDRPRGDIVLADGTVVAHSVETDGKFLYQRTYPFDDLYAHVVGSYSLMFGSDGVE
                     RRYTDELSGNTTDFRVGGFLDPFNETPNVGTVELSLVASVQEAARAALGTRNGSVVAI
                     NPQTGAVLAMWSYPTYNPNLTATNDAEAAKAFKEALDAAPGKPLLARTFRERFFPGST
                     FKIVTAAGALDAAKITETSPDFPVTTSYTPPLTSRPISNFGGSSCGGTLLEILEVSCN
                     APFARIGAELMGPQPLIAAAESFGFNEAAPIDLPGTARSVFPTDFGARIGSGAQPGAA
                     DLYEKTPQLAQSAIGQYEVAATPLQMALVAASVANDGRVMTPHVMNRILDRTGATVET
                     WEPSVWKESMTPATAEILRRAMQGVVAQGTATAMNIPGAVVGGKTGTAQLGTTPAKSH
                     AWVIGYAGPPNQAPSVAVAVLVEGQPGASEQTGGKVAAPIAKAVMEAALGAQAGTTPN
                     PPIGEGGD"
BASE COUNT          479 a         1018 c         1003 g          593 t
ORIGIN      
        1 cacgaccacg accaccacca ccaccacgac caccacaacg acctcgtcgc cctccaccac
       61 cgcggcgccc gagtcggtgg cccctgaatc ggtcgcgccc gagaccgttc cacccgaggc
      121 gacggcaccg agcggatgag cgttccaacg tctggcggcg cgcccgctcg aattcgtacg
      181 ccgcgttcgg tcgaggcggg actgttggtg ctcgccggac tgatcgtcgc tgcagcattt
      241 gttctcgtca gcctcggacg gtcccgcgcg ttgcccgccg acctggttcc gcttctggtg
      301 ggggttttcg tcctgttcgc tgctgcgcat atcggggttc gccgcctcgc tccgcgagcg
      361 gacgcgttgc tgttgccctc ggcggtgctg ctgaacggtt tggggtatgt gttcatcatg
      421 cgtctcgacc ccgatctcgg ggcgcaacag gcgggctgga ccgccgtcgg ggtggtcggg
      481 ttcatcggcg ttctggcggt gatccggcgc gttcgcaccc tggagggcct gcgattcacc
      541 gtcggcctcg tcggcgtgat cctgttgatc ctgccgctgt tgccgggcct gggggtcacg
      601 atctacgggg cgcggatctg ggtgcgggcg ggtccgatca gcttccagcc gggtgagttc
      661 gccaagctcg cactggccat cttctttgcc ggttacctcg tcgaacggcg cgagttgttg
      721 ggtaccgcga cgttcaaagt cggaccgttc atgatccccg aacccaagca tctggtcccc
      781 gtggtgctcg cttgggggac gagtctggtc gtgatggtgt tcgaacgcga cctcggttca
      841 gcgctgttgt tcttcgccct gttcatgtcg gtgttgtgga tcgcctcggg ccgaatctcc
      901 tacgtcgcga tcggctcgat gctgttcgcc gtgggcgcgg tgttcgcgtg gaactccttc
      961 ggccacgtcc gcacccgagt cgacgtgtgg ctcaatccat gggacgacgt gctggattct
     1021 ggctaccaga tcgcccaagc cacctacgcc tttgcgtggg gcggggtgac cggcgtcggg
     1081 ttcggtcagg gcttcggcaa tcgggtcccg gtggccgaaa gtgacttcat cttcgccatc
     1141 atgggtgagg aactcggcct gttgggcacg acggcggtca tcgtgttgtt cctcaccatc
     1201 atcggcagcg gatttcggat cgccatgcgt tcgacgaacc cgttcaacaa gttgttggcc
     1261 gtcggactca ccacgctgat cggctttcag tcgttcatca tcatggcggg cgtcacccga
     1321 ctgttgccgc tgaccgggct gacgttgccc tttgtgagtt acggcggatc ctcgctggtc
     1381 agcaactgga tgctcatcgc attgttgttg cgcctctccg atgaggccga gacgcgagag
     1441 gcgcaacggg tcgccgagga cgaccgggcg gccgacctcg gtctcaccac gacgatcggg
     1501 gtcggcgtgt gaggacctcg acgatgaatc gccagatcgt caagctcgtc gccgggctga
     1561 tggtctgtta tgtggcgttg ttcgcccgcc tcaaccagat ccaggtcttc gacgcgaaga
     1621 agctcaacaa gcgtgaggac aacacccgcg agatgatccg cgacttcgac cggccccgcg
     1681 gcgacatcgt gctggcggac ggaacggtgg tcgcgcactc ggtcgagacc gacggcaagt
     1741 ttctctacca gcgcacctac cccttcgacg acctgtacgc acacgtggtc ggctcgtatt
     1801 cgctgatgtt cggaagcgac ggggttgaac gccgctacac cgacgaactg tcgggaaaca
     1861 cgaccgactt tcgtgtcggt ggctttctcg atccgttcaa cgagaccccc aacgtcggaa
     1921 ccgtcgagct cagtctggtg gcctcggtgc aggaggccgc gcgtgccgcg ctcggtactc
     1981 gtaacggttc ggtcgtcgcg atcaaccctc agacgggcgc tgtgctcgcc atgtggtcgt
     2041 atccgaccta caacccgaac ctcacggcga ccaacgatgc cgaggcggcc aaggccttca
     2101 aagaagccct tgatgcggct cccggcaaac cgctgttggc ccggacgttt cgggaacggt
     2161 tcttcccggg ttcgaccttc aagatcgtca ccgccgccgg agccctcgac gccgccaaga
     2221 tcaccgagac ctctccggac tttcccgtca cgaccagcta cacgcctcca ctcacctccc
     2281 ggccgatcag caacttcgga ggatcgagct gcggtggaac gctgctggag attctcgagg
     2341 tgtcctgcaa cgcgccattc gctcgaatcg gtgccgaact catggggccc caaccgctga
     2401 tcgccgccgc ggaatccttc ggtttcaacg aggctgcgcc catcgatctg cccggcacgg
     2461 ctcgctcggt tttccccacc gatttcggag cgcgcatcgg gtcgggcgct cagccgggag
     2521 cggccgacct gtacgaaaag acgccgcagt tggcgcagtc ggcaatcggc caatacgagg
     2581 tggccgccac cccgctccag atggcccttg tcgcggcctc ggtggccaac gatggtcggg
     2641 tgatgactcc gcatgtgatg aatcggatcc tcgatcgcac cggcgcgacg gtcgagacct
     2701 gggagccgtc ggtgtggaag gaatcgatga cgcccgccac ggcggagatc ctgcgccgtg
     2761 cgatgcaggg tgtggtcgcc caaggtacgg cgaccgctat gaatattccc ggtgcagtcg
     2821 tcggcggaaa gaccggtacg gcacagcttg gcaccacacc cgcgaagtcg catgcgtggg
     2881 tgatcggcta tgcagggccg ccgaaccagg cgccgtccgt tgcggtcgcc gtgttggtcg
     2941 agggccagcc gggcgccagc gagcaaaccg gcggcaaggt ggctgctccg atcgccaagg
     3001 ccgtcatgga ggcggcactc ggtgcgcagg ccggcaccac cccgaacccc ccgatcggcg
     3061 aggggggaga ctgatgaccc cgaccaccga tcc
//
LOCUS       DAOOMA010000300         3088 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_38422_length_3088_cov_3.996044, whole genome shotgun sequence.
ACCESSION   DAOOMA010000300 DAOOMA010000000
VERSION     DAOOMA010000300.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 3088)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3088)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3088
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38422_length_3088_cov_3.996044"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..350)
                     /locus_tag="PLP95_08540"
     CDS             complement(<1..350)
                     /locus_tag="PLP95_08540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015439766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="steroid 3-ketoacyl-CoA thiolase"
                     /protein_id="HPB45884.1"
                     /translation="MAEAYIIDTLRTPVGKRGGSLSQVHPADLGAFTLKTLVERTGID
                     PSAVDDVVMGCLDTIGPQAGDIGRTAWLAAGLPEAVPGVTIDRQCGSSQQAVHFAAQG
                     VMSGTQDLVVAGGV"
     gene            454..1350
                     /locus_tag="PLP95_08545"
     CDS             454..1350
                     /locus_tag="PLP95_08545"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HPB45885.1"
                     /translation="MATNPSANNPSANTPTGNDSSPNQEQIDFWSGDRGKAWADSAER
                     YDLQLERYSLLAVGAAAPLPGDRAIDVGCGAGATTFEAARAVGPTGYVIGVDVSGPML
                     ERARHEQERTGLTNVEFVEADVQTMTPLTELADVVVSRFGVMFFDDPIAAFANLAAMV
                     RDRGRLGFACWGPASSNEWMSVPAVALAPIAPLPEPSPADAPGPFSFADQDRVESILE
                     AAGWSDVTFEVVDDPLYLGGPGSLAEVVEFVLGASSLSPLVADRREEARQLLTDALAP
                     RFDGHGVKFHANAHIVRAVREP"
     gene            complement(1510..2517)
                     /locus_tag="PLP95_08550"
     CDS             complement(1510..2517)
                     /locus_tag="PLP95_08550"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HPB45886.1"
                     /translation="MQFAFTPEQIEFADVVADLLVKECSPEQLRVAWDNESGTIPGLW
                     DRLVEMGVPGLLAPEGLGGFGLTELDLVRISYEAGRVALPEPLSATAGVVIPALRDHA
                     QSDDAERWIAAILGGEAQVALGLARNPFVLNASAAQTFLLEADDGLHLVDADVVEFAP
                     ETSVDHSRRPFRVEWTPSDATRLANAEVAAAEANTRSALFSAAELLGLAETMLSLTTG
                     YVAERKQFGVPIGSFQAIKHHCANALLKLEFAKPLVWRAAYSLATGDADGSVHVSMAK
                     AAASDAADVVGRIAMQCHGGIAYTVEYDLHLYLKRSWALIAQDGNAAAHRRRVADAVL
                     G"
     gene            complement(2527..>3088)
                     /locus_tag="PLP95_08555"
     CDS             complement(2527..>3088)
                     /locus_tag="PLP95_08555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012227972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HPB45887.1"
                     /translation="FAEVFFDGAFVPDGDSDPVNGGVLGEVGNGWGVAMATTGSERGL
                     TLRSPGRFTAAAARLVQLYRERGGTVDDPGVRDAVVQAWMDAEAYALYTLSDVTGIVE
                     GNAPGARSSLNKIFWSELDVRLHEASLRLLGGEAELEEWGPAGEQVENPWLKGYQFSL
                     SGPIYAGTNEIQRNIVAERVLGLPRK"
BASE COUNT          527 a         1082 c          988 g          491 t
ORIGIN      
        1 tgcacaccgc cggccaccac gaggtcctgg gtgccgctca tgaccccctg tgcagcgaag
       61 tgcacggctt gctgggacga accgcactga cggtcgatcg tcaccccggg aacggcctca
      121 gggaggcccg ccgcgagcca cgcggtacgg ccgatatcgc cggcctgcgg gccgatggtg
      181 tcgagacacc ccatcaccac atcatccacg gcggacgggt cgatccccgt gcgttcgacg
      241 agcgtcttca gcgtgaacgc accgagatcg gccggatgca cctgggacag cgagccgcca
      301 cgctttccga ccggggtacg cagggtgtcg atgatgtatg cctcagccat ctcgggcctt
      361 tctcgaacgc gatatctgac gttgtgtcag gttacgcctt cgccgcggcg gggtcgaagc
      421 cgaccacaac ccgatgtctg ccaggctgtc tgtatggcga ccaacccctc tgcgaacaat
      481 ccctctgcga acacgccgac cgggaacgac tcgagcccga accaggaaca gatcgacttc
      541 tggagcggcg atcgcggcaa agcctgggcc gacagcgccg aacgctacga cctccaactt
      601 gagcgttact cgttgctcgc tgttggtgcg gcggcgccgc tgccggggga tcgggcgatc
      661 gacgtcggat gcggcgctgg tgccaccacc ttcgaagcgg ctcgtgccgt cgggccgacc
      721 gggtacgtca tcggcgtcga cgtgtccggt ccgatgttgg aacgcgctcg ccacgaacag
      781 gagcgcaccg ggttgaccaa cgtggaattc gttgaggccg acgtgcagac gatgacgcct
      841 ctgaccgaac tcgccgacgt ggtcgtcagc cgctttggtg tcatgttctt cgacgacccg
      901 atcgccgcct tcgccaacct cgctgcgatg gttcgcgacc ggggccggct cgggttcgcc
      961 tgttggggac ccgcgagttc gaacgagtgg atgtcggtgc ctgcggttgc gttggctccg
     1021 atcgcaccgc ttcctgaacc gtctccggcc gatgcccccg ggcccttctc cttcgcagac
     1081 caggaccgcg tcgagtcgat tctcgaggcc gccggttgga gcgacgtcac cttcgaagtc
     1141 gtcgatgacc cgctgtatct gggagggccc ggcagcctcg ccgaggtcgt cgagttcgtc
     1201 ttgggcgcat cgtcgctcag ccccctggtg gcagatcggc gcgaggaggc acgccagttg
     1261 ctcaccgacg cactcgcccc ccgcttcgat gggcacgggg tgaagttcca cgccaacgcg
     1321 cacatcgtgc gcgccgtgcg cgagccgtaa accgccacca ccctgcccac agttcggccg
     1381 ccctgcccac agttccgccg ccctgcccac agtcgaactg tgggcagaac cggccgacag
     1441 gtgacccgcg ttgcccacag ttgaactgtg ggcaggaccg gtgcgagacc gagggcgaga
     1501 ccgaggtcgt cagcccagca cggcgtctgc gacacggcga cgatgcgccg cagcgtttcc
     1561 gtcctgagcg atcagtgccc acgaccgctt caggtacagg tggaggtcgt actcgaccgt
     1621 gtaggcgata ccgccgtggc actgcatcgc gatgcgaccc acgacatctg cggcgtccga
     1681 cgcggcggcc ttggccatcg acacgtgaac cgagccgtcg gcgtcaccgg tggccaacga
     1741 gtaggcggcg cgccacacga gcggcttcgc gaactcgagc ttcagcagcg cgttggcaca
     1801 gtggtgcttg atcgcctgga acgacccgat cggcacgccg aactgcttac gctcggcgac
     1861 gtatcccgtg gtgagcgaca acatcgtctc ggcgagcccc aacagttcgg ctgcgctgaa
     1921 caacgccgag cgggtgttcg cctctgccgc ggcaacctcg gcgttggcca gtcgggtcgc
     1981 gtcggagggg gtccactcca cacgaaacgg acgacggctg tgatccaccg aggtttcagg
     2041 cgcgaactcg accacatcgg cgtcgacgag atgcagccca tcgtcggcct cgagcaagaa
     2101 cgtctgcgcg gccgatgcgt tcaagacgaa cgggttgcga gcgaggccca gcgcgacctg
     2161 tgcctcgccg ccgagaatcg ctgcgatcca gcgctcggca tcgtcgcttt gcgcatgatc
     2221 gcgcagcgca ggaatgacca cgcccgcggt cgcgctcaac ggctccggca acgcaacgcg
     2281 tcccgcctcg tagctgatgc gcacgaggtc gagctcggtg agaccgaacc cgccgagacc
     2341 ttcgggggcc aacagcccag gcacgcccat ctcaacgaga cgatcccaca ggccggggat
     2401 cgtccccgac tcgttatccc aggcgacacg caattgttcg ggcgagcact ccttgacgag
     2461 gagatctgcc acgacgtcgg cgaattcgat ctgttcgggg gtgaaggcga attgcatggt
     2521 gacgacctac ttccgcggca gcccgagcac acgctcggcg acgatgttgc gctggatctc
     2581 attcgttccg gcgtagatgg gacctgacag cgagaactga taccccttca accaggggtt
     2641 ctcgacctgc tcgcccgcag ggccccactc ctcgagttcg gcctcgccgc ccagcaggcg
     2701 aagcgacgcc tcgtgcaacc gcacgtcgag ttcggaccag aagatcttgt tgagcgaact
     2761 gcgcgccccc ggtgcgttgc cctcgacgat gccggtcaca tcgctcagcg tgtacagcgc
     2821 ataggcctcg gcgtccatcc atgcctgcac gacggcgtcg cgcacgcccg ggtcgtcgac
     2881 cgtcccacca cgctcacggt agagctgcac cagccgggcc gcagcagcgg tgaaccgccc
     2941 cggggaacgc agggtcaacc ctcgctcgga cccggtggtc gccatcgcaa caccccagcc
     3001 gttgccgacc tcgccgagta ccccaccgtt gaccggatcg gagtcgccgt cgggcacgaa
     3061 cgcaccgtcg aagaacacct cggcgaac
//
LOCUS       DAOOMA010000301         3081 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_38548_length_3081_cov_3.312624, whole genome shotgun sequence.
ACCESSION   DAOOMA010000301 DAOOMA010000000
VERSION     DAOOMA010000301.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 3081)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3081)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3081
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38548_length_3081_cov_3.312624"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            80..778
                     /locus_tag="PLP95_08560"
     CDS             80..778
                     /locus_tag="PLP95_08560"
                     /inference="COORDINATES: protein motif:HMM:TIGR03936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03936 family radical SAM-associated protein"
                     /protein_id="HPB45888.1"
                     /translation="MKLRVRYTKVGKVRFLGNLDLARNWERAIRRAGLPVAYSEGFSP
                     RPKLHYGLALPMGAESLAEYLDVGLRDDIELDGVADALSAAMPVGIEVTDVADVPSDS
                     IALQAVVEHCTWRFDFDDTTSEVLADAAQRLLERDEVLLTFERKGKPITENVRPAVLD
                     LSVTTPPRHGVASLLATIATKPRSIRPSEFLTCLALEGDAPRISRIEQWMTIDGHPAP
                     LTMAGAPAVGASGT"
     gene            809..2731
                     /locus_tag="PLP95_08565"
     CDS             809..2731
                     /locus_tag="PLP95_08565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HPB45889.1"
                     /translation="MSETEPNAEALNAPLIPKIPPPAPPKPPTGRPVDIGEPPPGGGA
                     PSSASTGGTDDAIVGEPLSASAKRRRRRNRGGGAAGGGAQGQGQSPAQGQGQKQGQGQ
                     GQGQGHKQGQGQSGRPPQGDATASGASKGAAAGSATGLGSSEPDTDGRSDGGGESKTQ
                     GQGQAKKGGSRNRNRSRNRNRAAQPVEAVLTDEDGPLDIDPKSLKKRQGKSRNGRAVG
                     RYQMNVHVDSAGHTHIAVLEGRSLLEHYVSRPADDVSQIHGNIYLGRVENVLPGMEAA
                     FIDIATPKNAVLYRGDITRDADDVEVADDARIEDVLKPKQLILCQVTKNPIGHKGARL
                     TQEVSLPGRFVVLVPNSKTYGISKRLSDQERRRLRGILDRVKPEQHGVIVRTAAENVT
                     SEEIERDVTRLLKQWEQIEALAAKQKRPALLYREPDMAVRVIREEFNEDYRAVIIDDA
                     ALHRDVHDYVSAISPATADRVQFYNPKAEKLPLFERYQITEQLRKALDRKVWLPSGGS
                     LIIEHTEALTVIDVNTGRNVGSSSLEETVFRNNLEAAVEIARQLRLRDIGGIIVIDFI
                     DMEIKPNRDELVRTFKEALARDKTRTQVFAVSDLGLVEMTRKRIGEGLLESFAEQCSV
                     CNGRGVVLSSAVAGID"
     gene            2773..3081
                     /gene="rplU"
                     /locus_tag="PLP95_08570"
     CDS             2773..3081
                     /gene="rplU"
                     /locus_tag="PLP95_08570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012230392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HPB45890.1"
                     /translation="MYAVIKTGGKQYRVAEGDRVQVERLGEPGSDVSLTPVLVVDGDT
                     TLATPEQLKGASVSARIVDETKGPKINGFTYKNKSNNRRRWGHRQTLATIEITGITKG
                     "
BASE COUNT          617 a          978 c         1009 g          477 t
ORIGIN      
        1 gccaggggac cggagtcgat ctgagcgccg gcggtgccgt acccgtgtcg ctgctgtcgc
       61 gtcggccgga ggtgacggtg tgaagctgcg cgttcggtac accaaagtgg gcaaggttcg
      121 gttcctcggc aatctcgacc tggccaggaa ctgggaacgg gccatccgcc gcgccggatt
      181 gccggtggcc tacagcgagg gcttctcgcc ccgcccgaaa ctgcactacg gactcgcgct
      241 tcccatgggg gccgagtcct tggcggaata cctcgacgtc ggcctgcggg acgacatcga
      301 acttgatgga gtcgccgacg cgttgagcgc ggcgatgccg gtcgggatcg aggtgaccga
      361 tgtcgccgac gtcccgtccg attccatcgc cctgcaggcg gtggtcgaac actgcacctg
      421 gagattcgac ttcgacgaca ccacgtcgga ggtgctcgca gatgcggcac aacgcctgtt
      481 ggagcgcgac gaggtcctgt tgaccttcga acgcaagggc aagcccatca ccgaaaacgt
      541 ccgtccggca gtgctggacc tgagtgtgac gaccccgccg cgccatggcg tggcctccct
      601 cttggcgacc atcgccacca aacctcgcag tatccgaccg tcggagtttc tgacgtgcct
      661 cgccctcgag ggcgacgcgc cacggatcag ccgaatcgaa caatggatga ccatcgacgg
      721 tcacccggcg cccttgacca tggcgggcgc gccggccgtc ggagccagtg gaacatgaat
      781 ccacccggtc atccttcaca ggagcgacat gtccgaaacc gaaccgaatg ccgaagcgct
      841 caatgcgccg ttgatcccca agattccgcc gccagcacct ccgaaaccac cgacgggaag
      901 gcccgtggat atcggcgagc ctccgcccgg cggtggcgca ccgtcgtcgg cgagcaccgg
      961 cggaactgac gacgccatcg tcggcgaacc cctcagcgcg tcggcgaagc gtcggcgccg
     1021 ccgcaatcgc ggtggcggtg cagctggtgg tggcgcacaa ggccaggggc agtctccggc
     1081 tcagggtcaa ggccaaaaac aaggccaggg ccagggccag ggccagggcc acaagcaggg
     1141 tcaaggccaa tcagggcggc cgccacaggg cgacgccaca gcgtccggtg cgtcgaaggg
     1201 tgcggccgcg ggatcagcga cgggtttggg ctcgtcggag ccggatactg acggtcgaag
     1261 cgacggcggt ggcgagagca agacccaggg ccagggccag gcgaagaagg gtgggtcgcg
     1321 caaccgaaat cggtcacgaa accgcaaccg cgccgcgcaa ccggtcgagg cggttctcac
     1381 cgacgaggac ggtccgctcg atatcgaccc caagtcgttg aagaagcgcc aaggtaagag
     1441 ccgcaatggg cgcgccgtcg gccgatacca gatgaacgtg cacgtcgatt cggcggggca
     1501 cacccacatc gcggtccttg aagggcgttc gctgctggag cactacgtca gccgtccagc
     1561 cgatgacgtg agccagattc acggcaatat ctacctgggc agggtcgaga atgtgcttcc
     1621 gggaatggaa gcggccttca tcgatatcgc gacgccgaag aacgccgtgt tgtaccgcgg
     1681 cgacatcacc cgcgacgccg atgacgtcga agtggcggat gacgctcgca tcgaggacgt
     1741 gttgaagccc aaacagctca tcttgtgtca ggtgaccaag aaccccatcg ggcacaaggg
     1801 cgctcgactg acccaggagg tgtcgcttcc gggccgattc gtggtgctgg ttcccaactc
     1861 gaagacctac ggaatctcca agcgactgtc ggatcaggag cgccgacgcc tgcgcgggat
     1921 tctcgatcgg gtcaagcccg aacagcacgg ggtcatcgtt cgcacggccg ccgagaacgt
     1981 gacctccgag gagatcgaac gcgacgtcac ccgtttgctg aagcagtggg aacagatcga
     2041 agccctcgcc gccaagcaga agcgcccagc gctgttgtat cgcgagccgg acatggcggt
     2101 gcgggtcatt cgcgaagaat tcaacgagga ctaccgggcg gtcatcatcg atgatgcagc
     2161 gctgcaccgt gacgtccacg actacgtctc ggcgatctct ccagccaccg ccgaccgggt
     2221 tcagttctac aacccgaagg ccgaaaagct gccgttgttc gagcgctatc agatcaccga
     2281 acagttgcgg aaggcgctcg atcggaaggt ctggttgccg tccggcggat cgctgatcat
     2341 cgaacacacc gaggccctca cggtgatcga cgtcaacacc ggccgcaacg tcgggtcgtc
     2401 ctcgctcgag gagacggtgt ttcgcaacaa cctcgaggcg gcggtcgaaa ttgcgcgtca
     2461 gcttcgactc cgcgacattg gtgggatcat cgtgatcgat ttcatcgaca tggagatcaa
     2521 gccgaatcgc gacgaactgg tcaggacctt caaggaggcg ctggcccgcg acaagacccg
     2581 cacgcaggtc ttcgccgtca gcgaccttgg gttggttgag atgactcgaa agcgaatcgg
     2641 tgaaggtctg ctcgagagct tcgccgaaca gtgttccgtg tgtaacgggc gtggcgttgt
     2701 gctgtcgtcc gccgtcgcgg gcatcgacta gtttccccca gcccgcaggg cactgatagg
     2761 ctcaaggatc gtatgtacgc agtaatcaag accggtggta agcaataccg cgtggccgag
     2821 ggcgatcgcg tccaggtcga acgcctgggc gagcccggca gcgacgtgtc cctcacgccg
     2881 gtgctggtcg tcgacggcga caccaccctc gcaacgcccg aacagctgaa gggcgcatcg
     2941 gtttcggctc gcatcgtcga cgagaccaag ggccccaaga tcaacgggtt cacctacaag
     3001 aacaagtcga acaaccggcg ccgctggggt caccgtcaga cgttggccac catcgagatc
     3061 accggcatca cgaagggctg a
//
LOCUS       DAOOMA010000302         3079 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_38585_length_3079_cov_3.078373, whole genome shotgun sequence.
ACCESSION   DAOOMA010000302 DAOOMA010000000
VERSION     DAOOMA010000302.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 3079)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3079)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3079
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38585_length_3079_cov_3.078373"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..246)
                     /locus_tag="PLP95_08575"
     CDS             complement(<1..246)
                     /locus_tag="PLP95_08575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB45891.1"
                     /translation="MKLTGERPIEGKTPDSLLALHAAGYREVRDRLEPAGTFLDLGCG
                     LGDGTASFADGERVLVGIDYDIDTAVLASTDKADVGLM"
     gene            312..1568
                     /locus_tag="PLP95_08580"
     CDS             312..1568
                     /locus_tag="PLP95_08580"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HPB45892.1"
                     /translation="MVAASGDRPKTPVASPDTIATLAELSRRSGIRRVHMLAWRDLDD
                     VEAGGSEVHAHNIAAIWAQAGIEVTHRTSYAAGHPQRATRSGYRVIRKAGRYMVFPRT
                     VAAEIAGRMGPRDGLIEIWNGVPFMSPLWVRGPHAVWLHHVHGPMWQMSLPPGLAAAG
                     RFVEEKVAPRFYRRSPIVTLSNSSRDELVSEMGFDADHLTVVPPGVDQSYSPAGTKTE
                     APTVLAVGRLVPVKNFARLIRVMAMVRNSVPDAVLNIVGEGYERDDLETLIDDLDASQ
                     YIHLRGRLSDAELLELMRSSWCLASTSIREGWGMTLTEAAACGTPCVATRIAGHLDAT
                     VDGEAGLLGTTDAELVDHLVAVLTDAELRERLGRGAIKRAGELTWEAAAIATFEVLAA
                     TATPRGPHDLVDTSASTARPNPEDLP"
     gene            1565..2239
                     /locus_tag="PLP95_08585"
     CDS             1565..2239
                     /locus_tag="PLP95_08585"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HPB45893.1"
                     /translation="MTDTVVLVHGLATNAERTWRETGWIDLLGDFGVDVIAPDTLGHG
                     SAPQPTDPAAYDEFESYLLDAFPDEAVHAVGFSLGARTVLALALEHPERFDRIVLAGV
                     GENLFRRDGSSAELADALEAGDDGSNPMVQHFRQLAESSGQSIEALVAALRRPHPPLI
                     DEERLGTLQVPTLVVLGDADFAGPADRLVDAIPNAEFVSLRGVDHFATPKSMGFLDAA
                     FKFLGL"
     gene            complement(2255..>3079)
                     /locus_tag="PLP95_08590"
     CDS             complement(2255..>3079)
                     /locus_tag="PLP95_08590"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HPB45894.1"
                     /translation="SDACIETVRAFDAQGVDLRYLIADNASRPEHLEKLRAEFGRRGD
                     IAILQNTVNGGFSGGANLGLSAWLADREGSDWALLCPHDVALDPGCVRQLLDSARAEP
                     MAGLACADVGDGMTPYIDPYFGGMTMPGDPTPGWEPAAYPHGTLMALRRDCLVEVGLF
                     DASYFAYCEEAELALRARDAGWTCGLVRGARVRNVTIGSSVALVEYLQTRNTLRLVRS
                     MSGRYHATIRLIIHLGQALRWTVHPDKAPYIFDRRARLLGIRDFLAGRSGAPPADL"
BASE COUNT          506 a         1057 c         1024 g          492 t
ORIGIN      
        1 catcaacccg acgtcggcct tgtccgtgct ggccaacacc gccgtgtcga tgtcgtagtc
       61 gatgccgacc agaacccgct cgccgtcggc gaatgacgct gtgccatcgc cgaggccgca
      121 accgagatcc agaaaggttc ccgccggctc gagccggtcg cgaacctcgc ggtatcccgc
      181 cgcatgcagg gcaaggagcg aatcgggggt cttgccctcg atggggcgtt cgccagtgag
      241 cttcatcaat cctcggttca ggacctgcgg gtcgaccgac ccggcgggga acggatctag
      301 cataggcgcc cgtggttgct gcctccggag accgacccaa aacacccgtg gcctcgcccg
      361 acacgatcgc cacgttggcc gagttgtcac ggcgcagcgg aatccgacgc gtgcacatgt
      421 tggcgtggcg cgatctcgac gatgtcgagg ccgggggttc ggaggtgcat gcgcacaaca
      481 tcgccgccat ctgggcccag gccggcatcg aggtcaccca ccggacctcc tatgccgcag
      541 gacacccgca gcgagcgacc cgcagcggat accgcgtcat ccgcaaagcg ggacgataca
      601 tggtgtttcc gcgaacggtg gccgccgaga tcgccgggcg gatgggtcct cgcgacggcc
      661 tgatcgagat ctggaacggg gtgccattca tgtcgccgct gtgggtcagg ggcccgcatg
      721 cggtctggct tcaccacgtt cacgggccca tgtggcagat gtcgttgccc cccggtttgg
      781 cggcggccgg cagattcgtc gaagagaagg tggcgccgcg gttctatcgc cgctcaccca
      841 tcgtcacgtt gtcgaattcc tcgcgcgacg agttggtcag cgagatggga ttcgacgccg
      901 atcacctcac ggtggtgccg cccggggtcg atcagagcta ctcgcccgcc gggaccaaga
      961 ccgaggcccc caccgtgttg gcggttgggc gcctggtgcc ggtgaagaac ttcgcccgac
     1021 tcatccgggt gatggcgatg gtgcgcaact cggtacccga cgccgtgctg aacatcgtcg
     1081 gcgaaggtta cgaacgcgac gatctcgaaa cgctgatcga cgacctggac gcctcgcagt
     1141 acatccacct ccgaggtcgt ctgagcgacg ccgaattgct ggagttgatg cgctcgtcgt
     1201 ggtgcctcgc gtcgacctcg attcgggagg gctggggtat gaccctcacc gaggccgcgg
     1261 cgtgtgggac cccgtgtgtg gcgacgcgta tcgccgggca tctcgatgcg accgtcgacg
     1321 gtgaggccgg gctgctgggg accaccgatg cggaactggt cgaccacctc gtcgcggtgc
     1381 tcaccgacgc ggaactgcgc gaacgactcg ggcggggcgc catcaaacgg gcgggtgaac
     1441 tgacctggga ggccgccgcg atcgcgacct ttgaggtgct cgccgcaacg gcgacgcccc
     1501 gcgggcccca cgacctggtc gacacgtccg cctcaaccgc tcgccccaac ccggaggatc
     1561 tcccatgacc gacaccgttg ttctggttca cggcctcgcc accaacgcgg aacgcacgtg
     1621 gcgcgagacc ggctggatcg acctgctcgg cgacttcggc gtcgacgtca tcgccccgga
     1681 cacgctcggt cacggttcgg cgccgcaacc gaccgacccc gccgcctacg acgagttcga
     1741 aagctatctg ctcgacgcgt ttcccgacga agcggtgcac gccgtggggt tctccctcgg
     1801 cgcacgcacc gtgttggcgc tcgccctcga acatcccgag cgcttcgatc gcatcgtgtt
     1861 ggccggggtg ggcgagaacc tgttccgacg cgacgggtcg agcgcggaac tcgccgatgc
     1921 gctcgaggcg ggcgacgacg gatcgaaccc gatggtgcaa cactttcgcc agctcgcgga
     1981 gtcgagcggt cagtcgatcg aagcgctcgt cgccgccttg cgccgacctc acccgccgct
     2041 gatcgatgag gagcggcttg gcacccttca ggtgccgaca ctcgtcgtcc tcggcgacgc
     2101 cgatttcgcc ggaccggcag accgtctcgt cgacgccatc cccaacgccg agttcgtctc
     2161 cctgcgcggc gtcgaccact tcgcaacccc gaagtcgatg ggctttctcg acgccgcgtt
     2221 caagttcctc ggcctgtagc gccgcgcctc ggactcacag atcggccggc ggcgctccgc
     2281 tgcgtccggc caggaaatcg cgtatgccca gcaaacgggc gcgacggtcg aaaatgtagg
     2341 gcgccttgtc ggggtgcacc gtccatcgca atgcctggcc cagatggatg atcagccgaa
     2401 tcgtggcgtg gtaccggccc gacatcgacc ggacgaggcg cagggtgttg cgggtctgga
     2461 gatactcgac cagcgccacc gacgatccga tggtcacgtt gcggacccgg gcaccgcgaa
     2521 ccaacccgca ggtccaaccg gcatcacggg cccgcaacgc cagttccgcc tcctcgcagt
     2581 aggcgaagta ggaagcatcg aacagaccga cctctacgag gcagtcacgc cgaagcgcca
     2641 tcagcgtgcc gtgggggtag gccgctggtt cccagccggg ggtcgggtct ccgggcatgg
     2701 tcatgccgcc gaagtacggg tcgatgtagg gggtcatgcc atcgccgaca tcggcgcatg
     2761 ccagcccggc catgggttcc gcgcgcgccg aatcgagaag ctggcggaca caacccgggt
     2821 cgagtgcgac gtcgtgaggg cacaacaagg cccagtcgct gccctcgcgg tcggcgagcc
     2881 aggcggacag gcccaggttc gcacccccgc tgaacccgcc gttcaccgtg ttctgcaaga
     2941 tggcgatatc tcctcggcgg ccgaactccg cgcgcagctt ttcgaggtgt tcgggtctcg
     3001 acgcgttgtc ggcgatcagg taccgaaggt ccacgccctg cgcgtcgaag gcccgcaccg
     3061 tctcgatgca ggcatcgga
//
LOCUS       DAOOMA010000303         3071 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_38703_length_3071_cov_3.845159, whole genome shotgun sequence.
ACCESSION   DAOOMA010000303 DAOOMA010000000
VERSION     DAOOMA010000303.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 3071)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3071)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3071
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38703_length_3071_cov_3.845159"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(99..1529)
                     /locus_tag="PLP95_08595"
     CDS             complement(99..1529)
                     /locus_tag="PLP95_08595"
                     /inference="COORDINATES: protein motif:HMM:NF013434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HPB45895.1"
                     /translation="MRDQLDAVVVGAGPNGLAAAVAIARAGYSVAAFEAADTISGGAR
                     SGELTVPGLVHDLCSASHPFGVASPFLSALPLEQHGLEWVWADVDLAHPLDDGTAAFA
                     YRDFDRTCDGLGEDGPRWRSVFGPLLSTFDALTGDIMGPILRWPDHPVSMARFGLRAA
                     MPATAIGRRFRTERARALWMGNAAHLYHRLDRPLSSSVGVMLTLAGHAHGWPVPRGGS
                     AAITAALASLLESHGGTVTTGMHVRSLEELPSSRVVILDVAPGAAVELLGDRLPTRRQ
                     RALRRFRHGPAAFKVDFAVRNGVPWTAEKVGSAGFVHLGGSAADIASGEAATVRGDMS
                     QRPFVLVGQQHVADPGRAHDDLVPIYAYSHVPAGWSGDGESIIIDQIERFAPGFRERI
                     VATSVQRPADLQRDNPNLVDGDIVGGSNDPMQLLARPRLARDPYSTGAPGVFLCSAST
                     PPGAGVHGMNGYHAATRALRLLGEAR"
     gene            complement(1540..>3071)
                     /locus_tag="PLP95_08600"
     CDS             complement(1540..>3071)
                     /locus_tag="PLP95_08600"
                     /inference="COORDINATES: protein motif:HMM:NF023500.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3556 domain-containing protein"
                     /protein_id="HPB45896.1"
                     /translation="VAVGWAFIMTTPGVDGWFEPSSWWQRPEAFYKFALWTMLFEVLG
                     LGCGFGPLNLRFLPPMGSALYWLRPGTIRLPPFRGRIPGTGGDTRSVVDAALYAALLA
                     SIVVALIGALTRWEVAVVVGLLAVVGLRDQVIFLAARGEVYGSIAVTFLFATTDQVTG
                     AKFVMLAIWCGAAASKLNRHFPFVMAAMQSNSPMWRFGGVKRRLYRSFPEDLRPSGFA
                     ATLAHGATVIEFVAPAVLMFSGGGTITTVAAIVMIVFHLGIISSFPMGVPLEWNVFMI
                     WGVVFLFLAHPGVELATLSQPWWILVLTAALIGLVVYWNLVPARVSFLLSMRYYAGNW
                     AATVWCFRGDSLERLDRGVTKATMLPHQQLERVYGSAEEAAIPLFMGYAFRGFHTHGR
                     ALWALVPRACGPDHEDYLVLDGELVAGTVLGWNFGDGHLHDERLIDALQRRCDFRPGD
                     VRVVILESQPFHRGTQRYRLIDAATGLIETGHVNVADMIVRQPTDDDLPLHPDQVAQP
                     T"
BASE COUNT          577 a         1028 c         1036 g          430 t
ORIGIN      
        1 tccaagccac ccgacccgac aacaccacca tcaccaacac cccccgccac aaagagccgc
       61 cccaacctcc gggctgacac cccaacccct gggctgagtc agcgtgcttc gccgagcagg
      121 cgcagcgctc gggtggcagc gtgatagccg ttcatcccgt gaactccggc gccgggcggt
      181 gtggacgcgg aacacaggaa cactccgggc gccccggtcg agtacgggtc gcgggcgagg
      241 cgagggcgag cgagtagttg catcgggtcg ttcgatcctc cgacgatgtc tccgtcgaca
      301 aggttggggt tgtcgcgctg caggtcggcg ggtcgctgca ccgaggtggc cacgatgcgc
      361 tcgcggaatc cgggagcgaa ccgctcaatc tggtcgatga tgatcgactc tccgtcgccg
      421 ctccagccgg cgggaacatg ggagtaggcg tagatcggca cgagatcgtc gtgtgcacgc
      481 ccgggatctg cgacgtgttg ctggccgacc aggacgaacg gtcgctgaga catgtcgccc
      541 cgaaccgtcg ccgcctcgcc ggacgcgatg tctgccgcgg aacctccgag gtgaacgaag
      601 ccggcgcttc ctaccttctc cgcggtccac gggacaccgt tgcgtacggc aaagtcgacc
      661 ttgaaggcag ccggaccgtg acgaaagcgc cgcaaggcgc gctggcgccg tgtgggaagc
      721 cggtctccga ggagttcgac cgctgcaccg ggagcgacat cgaggatgac gacccggctc
      781 gacggcaact cctcgagcga ccgcacgtgc attccggtgg tgacggtgcc tccgtgagat
      841 tccaacagtg acgccagcgc ggcggtgatc gccgcggatc cgcccctcgg aaccggccaa
      901 ccgtgtgcgt gcccggcgag ggtcaacatc accccgaccg aggaggacaa gggccggtcg
      961 agccggtggt acagatgcgc ggcattaccc atccacagcg cccgcgcccg ctcggtgcgg
     1021 aaccttcgcc cgatcgcggt ggccggcatc gcggcgcgca ggccgaagcg ggccatcgac
     1081 accgggtgat cgggccagcg caggatgggc cccatgatgt cgccggtgag cgcatcgaag
     1141 gtcgacagca acggcccgaa caccgagcgc catcggggcc cgtcctcgcc gagcccgtca
     1201 caggttcgat cgaagtcgcg gtaggcgaac gctgcggtgc cgtcgtcgag cgggtgggcg
     1261 agatcgacgt cggcccagac ccactcgagg ccgtgttgct cgagtggcag cgccgacaga
     1321 aatggcgacg ccacaccgaa cgggtgcgag gccgaacaga ggtcgtggac gaggccgggc
     1381 acggtcagtt cgccggaccg agcaccgccg ctgatcgtgt cggcagcctc gaaggccgcg
     1441 acgctgtatc ccgcacgggc gatcgcgacc gccgcggcaa gcccgttcgg cccggccccg
     1501 acgacgacgg cgtcgagttg atccctcatg tcggccggct catgttggct gggcgacctg
     1561 gtcggggtgc agcggcaggt cgtcgtcggt cggttggcgc acgatcatgt cggcgacgtt
     1621 cacgtgtccg gtctcgatca acccggtcgc ggcgtcgatc agccggtacc gctgggttcc
     1681 ccggtgaaac ggctgcgact cgagaatcac gacgcgcaca tctccgggtc ggaagtcgca
     1741 gcgccgttgc aacgcgtcga tgagtcgttc gtcgtggagg tggccgtcgc cgaagttcca
     1801 cccgagcacg gtcccggcga cgagttcgcc gtcgagcacg agatagtcct cgtggtcggg
     1861 gccgcaagcc cgcggcacca gcgcccacaa cgcacgtccg tgggtgtgaa agccgcggaa
     1921 cgcgtatccc atgaagagtg ggatcgccgc ttcctcggca ctgccgtaga ccctttcgag
     1981 ttgttggtgg ggcagcatcg tcgccttggt caccccgcgg tcgaggcgtt cgagcgagtc
     2041 gccgcggaaa caccacacgg tggcggccca gttgccggcg taataccgca tcgacagcag
     2101 aaacgagacc ctggccggaa cgagattcca gtacacgacc aacccgatca acgcggccgt
     2161 gagcacgagg atccaccacg gctggctcag tgttgcgagt tcgacaccgg ggtgggcgag
     2221 gaacaggaaa acgactcccc agatcatgaa cacgttccat tcgagcggga ctcccatcgg
     2281 aaagctcgaa atgatgccga ggtggaaaac gatcatcacg atcgccgcga cggtcgtgat
     2341 ggtcccgccg ccgctgaaca tcaacaccgc cggggccacg aactcgatga ccgtcgcacc
     2401 gtgagccagt gtcgcggcga acccgctggg tcgcagatcc tccgggaagc tgcgatacag
     2461 gcgtcgtttg acgccaccga atcgccacat cgggctgttc gactgcatcg ccgccatgac
     2521 gaaagggaag tggcggttca gtttcgatgc ggccgctccg caccagatcg ccaacatgac
     2581 gaatttcgct ccggtgacct ggtcggtggt ggcgaacagg aaggtgaccg caatcgagcc
     2641 gtagacctcg ccgcgcgccg cgagaaagat cacctgatcc cgaagtccca ccacggccaa
     2701 cagccccacg acgaccgcga cctcccagcg ggtcaacgcc ccgatcaagg cgacgacgat
     2761 cgatgcgagc agcgccgcgt acaacgcggc atcgacgacg cttcgagtgt cgccgccggt
     2821 gccgggaatc cggcctcgaa acggtggcag ccggatcgtg cccgggcgaa gccagtacag
     2881 cgccgaaccc atgggtggga ggaaacggag attgaggggg ccaaagccgc agccgaggcc
     2941 gagcacctcg aacaacatcg tccagagggc gaacttgtag aacgcctccg ggcgctgcca
     3001 ccacgaactg ggttcgaacc agccgtcgac gccgggagtt gtcatgatga acgcccaccc
     3061 gacggcgacg a
//
LOCUS       DAOOMA010000304         3071 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_38707_length_3071_cov_3.617042, whole genome shotgun sequence.
ACCESSION   DAOOMA010000304 DAOOMA010000000
VERSION     DAOOMA010000304.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 3071)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3071)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3071
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38707_length_3071_cov_3.617042"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..350)
                     /locus_tag="PLP95_08605"
     CDS             complement(<1..350)
                     /locus_tag="PLP95_08605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020378816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HPB45897.1"
                     /translation="MATITDSTPHLPGEALESSRMPGHWLLARLGKRVLRPGGVELTS
                     KVLADLRVSRGDDVVEVAPGLGATTRLILESAPASYTGVDRDPAAADLVEAMLAGPNR
                     RVVQASAADTGLADA"
     gene            511..1743
                     /locus_tag="PLP95_08610"
     CDS             511..1743
                     /locus_tag="PLP95_08610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HPB45898.1"
                     /translation="MEEQLAQAAGHGDMAWILVSAALVLFMTPGLAFFYGGMVRTKNV
                     LNMFMMNFWCLLVIPLLWVVAGYSLAFSGDGKFLGNLDNAMFKNFDFASIDPLWAIFL
                     ATFAAITPALISGAVADRIKFSAWAVFVPIWSLLVYVPVTYWIYGGGGWLKDRGSLDF
                     AGGTTIHINAGIAALAMILVLGKRKGWPEKIPTPHNMPFVMLGTGILWLGWFGFNAGS
                     AGAANGVAAQAWINTLVAGAAAGLAWSLTEKVRDGHFTNLGVASGIVAGLVIITPAAG
                     FVTTGSAILMGLIGGPICALAVGLKTKLGYDDALDVIGVHFIGGLVGSILLGFFADSA
                     GLFNEGGFSDGVFFGGGTHLLVEQIVANVVTIVYSFIVTFAIAKVLDKTIGLRVDEDV
                     EIEGLDIREHAETAYSTL"
     gene            1765..2103
                     /locus_tag="PLP95_08615"
     CDS             1765..2103
                     /locus_tag="PLP95_08615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012224972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HPB45899.1"
                     /translation="MKLVTAIIKPFRLDGVKEALKSIGIEGLTVAEVQGFGRQGGHTE
                     TYRGAEYQIDFVPKIKIEAVVDDAVADQVIDTIVNAARTDKIGDGKVWVTEVETLVRI
                     RTGERGADAL"
     gene            complement(2249..2884)
                     /locus_tag="PLP95_08620"
     CDS             complement(2249..2884)
                     /locus_tag="PLP95_08620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HPB45900.1"
                     /translation="MSVELVTTDGIFSLDGEDFEVTNNIWIVGDDLEVVVIDAAHDHR
                     RIVDAVGGRRVRQILLTHGHNDHINAAGALADAVDAPISLHPDDTMLWEVVYPDRRVD
                     IALAEGQVISAGGHELGVLHTPGHAPGCCCFHDIASATLYSGDTLFCGGPGATGRSFS
                     DRPTILRTIRQRLLTLPDETVVHTGHGDSTTIGNERELIEDLVRTELGAEG"
     gene            complement(2881..>3071)
                     /locus_tag="PLP95_08625"
     CDS             complement(2881..>3071)
                     /locus_tag="PLP95_08625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="S-(hydroxymethyl)mycothiol dehydrogenase"
                     /protein_id="HPB45901.1"
                     /translation="WYGDCLPERDFPMLIDLYLQGRLDLDRFVSETISLDGVEEAFHK
                     MERGEVLRSVVTIDGAPS"
BASE COUNT          570 a         1035 c          911 g          555 t
ORIGIN      
        1 gcatcggcga gtccggtgtc ggcagcactc gcctgcacca cccggcggtt cggaccggcc
       61 agcatcgcct cgacaaggtc ggccgccgcc ggatcccggt cgacgcccgt gtaactcgcc
      121 ggtgccgact ccaggatcag cctggtcgtc gcccccaacc cgggggccac ctcgaccacg
      181 tcatcgccgc ggctcactcg caggtcggcg aggaccttcg aggtcaactc gacgccgccg
      241 gggcgaagga cacgcttacc caatctggcc aagagccaat gtcccggcat tcgcgaggac
      301 tcgagcgcct cgccgggaag atgcggtgtg gaatcagtga tggtcgccat ggtgtaaagc
      361 taccctaata tccgtcgatt acaagaccta cagcccttcg aacgagccgc gcaacctgtt
      421 cacagagcag aaacatccac gaagcggcac cgcaatcgac cctctgcaga ctgctcctcg
      481 caactcctcg aacgaaagtg agtaactgcg atggaagagc aactcgcaca agcagcggga
      541 cacggagaca tggcttggat cctggtttct gccgcactcg tgctgttcat gacccccggc
      601 ctggccttct tctacggagg catggtccgg accaagaatg tcctcaacat gttcatgatg
      661 aacttctggt gcctgttggt gatccccctg ttgtgggtcg tcgccggcta cagcctcgcc
      721 ttttctggag acggcaagtt cctcggcaac ctcgacaatg cgatgttcaa gaacttcgac
      781 ttcgccagca tcgatccgct ctgggcgatc ttcctggcga ccttcgctgc gatcaccccg
      841 gcgctcatct ccggcgctgt ggccgaccgc atcaagttct ccgcatgggc ggtcttcgtg
      901 ccgatctgga gcctgctcgt ctacgtcccg gtgacctact ggatctatgg cggcggcgga
      961 tggttgaagg accgtggctc cctcgacttc gccggcggca ccacgatcca catcaatgcc
     1021 ggtatcgccg cattggccat gatcttggtc ctcggcaagc gcaagggctg gccagaaaag
     1081 atcccaaccc cgcacaacat gcccttcgtc atgctgggaa ccggcatcct gtggctcggc
     1141 tggttcggat tcaacgctgg ctcggccggc gctgccaacg gcgtcgcagc ccaagcctgg
     1201 atcaacacgc tcgtcgccgg cgcagcagca ggcctcgcct ggagtctcac cgagaaggtc
     1261 cgtgacggtc acttcaccaa cctcggcgtc gcctctggca tcgttgccgg cctcgtcatc
     1321 atcaccccgg ccgccggctt tgtgaccacc ggttcggcca tcctcatggg cctcatcggc
     1381 ggcccgatct gcgccctcgc agtcggcctc aagaccaagc tcggctacga cgacgcactc
     1441 gacgtcatcg gcgttcactt catcggcggg ctggtcggct cgatccttct cggcttcttc
     1501 gccgacagtg ccgggctgtt caacgagggc ggcttctccg acggcgtgtt cttcggcggc
     1561 gggacccacc tgctcgtcga acaaatcgtg gccaacgtcg tcacaatcgt ctattccttc
     1621 atcgtcacct ttgcgatcgc caaggtgctc gacaagacca tcggcctgcg ggttgacgaa
     1681 gatgtcgaga tcgaaggtct cgacatccgc gaacatgcag aaaccgccta cagcaccctc
     1741 tgatcgtctc gaaaggacac gtacatgaag ctcgtaaccg caatcatcaa gcccttccgg
     1801 ctcgacggag tcaaggaggc gctcaagtcc atcggcatcg aaggcttgac cgtcgccgag
     1861 gtccaggggt tcggacgcca aggtgggcac accgagacct accgaggcgc cgaataccag
     1921 atcgacttcg ttccgaagat caagatcgag gccgtcgtcg acgacgcagt ggccgaccag
     1981 gtcatcgaca ccatcgtcaa cgccgcacga accgacaaga tcggtgacgg caaggtctgg
     2041 gtcaccgagg tcgaaaccct cgttcgaatc cgcaccggcg agcgcggagc cgacgccctg
     2101 tagcgggttc agcgcactcc gccaccgttc ttgatccatt cgagaaaggg ggtatccaac
     2161 gccgatgggg gtcccgcaca acacgtgcgg gacccccatc atttttgggt cagccttgcg
     2221 tcggttcagg caccgcgtcg gtatgggctc agccctcggc gccgagttcg gttctgacca
     2281 ggtcctcgat cagttcgcgt tcgttgccga tcgtggtgga atcgccgtgg ccggtgtgga
     2341 ccacggtctc gtcgggcagg gtcaacagac gctgccggat cgtacgcagg atcgtggggc
     2401 ggtccgagaa actccgaccg gtcgcaccgg gcccgccgca aaacagcgtg tcgcccgaat
     2461 acagcgtggc cgacgcgatg tcgtgaaagc agcaacaccc gggcgcgtga cccggtgtgt
     2521 gcaacacccc gagttcgtgc ccgcccgccg agatcacctg cccctcggcc aacgcgatgt
     2581 cgacgcgacg gtccggatac accacctccc acaacatcgt gtcgtcgggg tgcagcgaga
     2641 tcggtgcgtc caccgcgtcg gcgagcgcgc ccgcagcgtt gatgtggtcg ttgtgaccgt
     2701 gggtcaacag gatctgacga acccggcggc caccgaccgc gtcgacgatc cggcgatggt
     2761 cgtgagccgc gtcgatgacc accacctcca agtcgtcgcc gacgatccag atgttgttgg
     2821 tgacctcgaa gtcctcgccg tcgaggctga aaattccgtc ggtggtgacg agttcgacgc
     2881 tcatgatggc gcaccgtcga tcgtgacgac cgaacgcaac acctcgccac gctccatctt
     2941 gtggaacgcc tcttccacgc cgtcgagcga aatggtctcg ctcacgaaac ggtcgaggtc
     3001 gagccggccc tgcagataca ggtcgatgag catcggaaag tcgcgctcgg gaagacagtc
     3061 tccgtaccag g
//
LOCUS       DAOOMA010000305         3036 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_39289_length_3036_cov_3.787655, whole genome shotgun sequence.
ACCESSION   DAOOMA010000305 DAOOMA010000000
VERSION     DAOOMA010000305.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 3036)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3036)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3036
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39289_length_3036_cov_3.787655"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..483)
                     /locus_tag="PLP95_08630"
     CDS             complement(<1..483)
                     /locus_tag="PLP95_08630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB45902.1"
                     /translation="MINWINRLPPYSGAGLLLVADGGATNCRVAICSADGTLIGYHKA
                     HGRPTNARVIGEAAAGENLVFTANAAMRSAGVSTADIETCLITSASVDTLARAASFDD
                     ALGAAGLAHAVRSTVPDTMGCWASTDSLGPAAAIIAGTGSVAVAADRERRIWRRFGGW
                     D"
     gene            complement(529..1398)
                     /locus_tag="PLP95_08635"
     CDS             complement(529..1398)
                     /locus_tag="PLP95_08635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB45903.1"
                     /translation="MSALDYTLIGHVCIDELDDGTDRLGGTVHFAAGQALALGCEVRV
                     ITSATARLAQRLFDAHDGAIELDLRESDGDTCFGFDPTADDGPARLLSAAAPIDAGAV
                     AMLLAAADRGTVHLGPIAGELGPGVVEMARAGSDYLGITPQGMLRAFAEDGSFVVDPS
                     GWHDAVLSADALVLNDGEYRRVDQLGLLAGYDGLVFRTLGRDGALVMRGGTVLAQYAS
                     ALGGHLEGANDRPRPVNPDRTIGAGDVFAAAAFIAHAAGESPADALEVAVELASHWVH
                     RDSETPCWFGIDS"
     gene            complement(1559..1684)
                     /locus_tag="PLP95_08640"
     CDS             complement(1559..1684)
                     /locus_tag="PLP95_08640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB45904.1"
                     /translation="MSKSLRRNAPLIASIAGLAVFSTGCTIDDVLTIAKIVALFI"
     gene            complement(1807..2358)
                     /locus_tag="PLP95_08645"
     CDS             complement(1807..2358)
                     /locus_tag="PLP95_08645"
                     /inference="COORDINATES: protein motif:HMM:NF020348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding beta-barrel domain-containing
                     protein"
                     /protein_id="HPB45905.1"
                     /translation="MSEWGPLAALVGDWEGTGGLDTAFSHSEEQVINTPYFEKVEMKP
                     FGPVANGDQQLWGLDYHSAMWRESEDIPFHTEVGYWLYDPATGEILRGFVVPRGITVL
                     AGGQADADAKQFTMSARAGDPLYPIGENRYLAERASTRLYTVTVTLNDDDTWSYAETT
                     LLQMTEIPGELAHNDANTLHRRN"
     gene            complement(2464..>3036)
                     /locus_tag="PLP95_08650"
     CDS             complement(2464..>3036)
                     /locus_tag="PLP95_08650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB45906.1"
                     /translation="LIGELRDRGLKLPGIRALIEEGDVSERVADWLGLDTQLRGSWTG
                     IEPRIIGNKELKEALDGMPAGSRGALEDGRLIVRQGDSWLLPNPALYDLTLRLIASGV
                     PGDSVLEAGEILRGALAVAAKELIELFVTMVTHGYGRGIDPATLVNAFKPSAGDGARI
                     IFEQQLEHAIDDLLNDTKRISSLRSSAERN"
BASE COUNT          515 a         1056 c          966 g          499 t
ORIGIN      
        1 gtcccagcct ccgaatcgac gccagatccg ccgctcgcgg tcggccgcga ccgctacgct
       61 gccagtgccg gcgatgatcg ccgccgccgg acccagcgaa tcggtcgagg cccagcaccc
      121 catcgtgtcg ggcaccgtcg acctcaccgc atgcgccaac ccggctgcgc cgagcgcgtc
      181 gtcgaagctg gccgctcggg ccaacgtgtc gaccgaggcg ctcgtgatca ggcaggtctc
      241 gatgtccgcg gtcgacaccc cggcggagcg catcgcggcg ttcgcggtga agacgaggtt
      301 ctcccccgcg gcggcctcgc cgatgacccg ggcgttcgtc ggacgaccgt gcgccttgtg
      361 gtatccgatg agggttccgt cggccgaaca gatcgcgact cggcagttcg tcgcacctcc
      421 gtcggccacc agcaacagcc cagcgccgct atagggggga agtcgattga tccagttgat
      481 catcccagca ccatactcag cggccggtcg ctgtccgtcg agtagcgctc aggagtcgat
      541 gccgaaccaa cacggcgtct cggagtcacg atgcacccaa tggctcgcca gctcgaccgc
      601 cacctcgagt gcatcggcgg gcgattcccc ggcggcgtga gcgatgaatg ccgccgcggc
      661 gaacacgtct ccggcaccga tcgtacggtc gggattcacc ggtcgaggtc gatcgttcgc
      721 cccctcgagg tgcccgccga gcgcggacgc atactgcgcc aacacggttc cgccgcgcat
      781 caccaatgcc ccgtcgcggc cgagcgtgcg aaacaccaac ccgtcgtagc cggccaacag
      841 gccgagttgg tcgacgcgac ggtattcgcc gtcgttgagc accagcgcgt ccgcgctcaa
      901 gacggcgtcg tgccatccgc tcgggtcgac gacgaaggaa ccgtcctcgg cgaaggctcg
      961 cagcattccc tgtggggtga tgcccagata atcggacccg gcgcgcgcca tttccacaac
     1021 accgggaccc aactcacccg cgatcgggcc gagatgcacg gtgccccggt cggcggcggc
     1081 caacaacatg gccaccgctc cggcatcgat gggtgcggcc gcgctcagga gccgggctgg
     1141 tccgtcatcc gcggtggggt cgaaaccaaa gcacgtatcg ccatcggact cgcgcagatc
     1201 gagttcgatc gccccgtcgt gggcatcgaa aagtcgttgc gccaaccgtg cggtcgcgct
     1261 ggtgatcacg cggacctcac acccgagcgc caacgcctga cccgcagcga aatgcacggt
     1321 gcccccgagg cggtcggtgc cgtcatccaa ctcgtcgatg caaacgtggc cgataagcgt
     1381 gtagtcgagt gcggacatgg ctgcacccta ccgaagccca tccaaaccca ccgaaggcgc
     1441 cgtcgtcgtg tcggactcgc cgccgactcc accaccctgg ccgaaaaaac gccacggggc
     1501 accccgagtt gacggagtgc cccgtggatg gcgatgcggc aaaactgccg caagcaacct
     1561 agatgaacag tgcgacgatc ttggcgatgg tcaacacgtc gtcgatggtg cagccagtgc
     1621 tgaacaccgc caggccggcg atcgatgcga tgagcggcgc attacgcctc agcgacttgg
     1681 acatgtggca atccctcccg agtgccgaga ctgggcgtct cggatccgtc gacggtacca
     1741 aacccacctc ccatgcggaa gcccttcacg gcatttcgtt cacattcggg ctcgcgggcc
     1801 cgagtgtcag ttgcgtcgat gcagggtgtt cgcgtcgttg tgcgccagtt cgcccggaat
     1861 ctccgtcatc tgcaacaggg tcgtttcggc gtaggaccag gtgtcatcgt cgttcagcgt
     1921 gacggtgacc gtgtacagcc gggtcgaggc gcgttcggcg aggtagcggt tctcgccgat
     1981 cgggtagagc ggatcgccgg cgcgtgcgga catcgtgaac tgcttggcgt cggcatcggc
     2041 ctggccgcct gcgagtaccg tgattccgcg gggcacgacg aatccgcgca ggatctcacc
     2101 ggtcgccggg tcgtacagcc agtaaccgac ctcggtgtga aacggaatgt cttcgctctc
     2161 ccgccacatg gccgagtgat aatcgaggcc ccacagttgc tgatcgccgt tggccacggg
     2221 cccgaacggc ttcatctcga ccttctcgaa atacggcgtg ttgatcacct gttcctcgct
     2281 gtgcgagaag gcggtgtcga gtccaccggt tccctcccag tcgccgacca acgcggccaa
     2341 tgggccccat tcgctcatat cgatgtcctt tcccgtgggg tgctcgcgtg cggcacgcgc
     2401 ccgccgccgg gaccctagtg ccaggggccg ccacggggcc gagaatcgcg gcgagcagcc
     2461 gaatcagttg cgctcggcgc tcgatcgcag gctggagatc cgcttcgtgt cgttcagcag
     2521 gtcatcgatc gcatgctcga gttgctgttc gaaaatgatg cgcgcaccat ctccagcgga
     2581 aggtttgaag gcgttgacca acgtcgccgg atcgatcccg cggccgtacc cgtgggtgac
     2641 catcgtgacg aacaactcga tcagttcctt tgccgccacg gccagcgcgc cccggagaat
     2701 ctcgcccgcc tccagcacgc tgtcgccggg aacgcccgac gcgatgaggc gcagggtcag
     2761 gtcgtacaac gcggggttcg gcaggagcca cgagtcgccc tgacgcacga tgagacggcc
     2821 gtcctccagc gcaccacggc tgcccgccgg catgccgtcg agcgcttctt tgagttcctt
     2881 gttgccgatg atgcgcggct cgatcccggt ccacgatccg cgcagctgcg tgtcgagtcc
     2941 gagccaatcc gcgacccgct cggacacgtc gccctcctcg atgagcgcgc ggattcccgg
     3001 cagtttcagc ccacgatcgc gcagttcgcc gatgag
//
LOCUS       DAOOMA010000306         3035 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_39310_length_3035_cov_4.058725, whole genome shotgun sequence.
ACCESSION   DAOOMA010000306 DAOOMA010000000
VERSION     DAOOMA010000306.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 3035)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3035)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3035
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39310_length_3035_cov_4.058725"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1028)
                     /locus_tag="PLP95_08655"
     CDS             complement(<1..1028)
                     /locus_tag="PLP95_08655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HPB45907.1"
                     /translation="MTRPIFTDDHEMFREAYRSFVERSLVPRREEWEAAGIVSRDVWE
                     EAGAAGFLAFDTPEEYGGPGVKDFRYNLVVVEELARAGAMATGAGFTLHSDVALPYFL
                     RYCNDEQKQRWLPGMANGTLITAIAMTEPAMGSDLAGMQTTAIRDGDTYLLNGSKTFI
                     TNGINSDLVIVAAKTDPTQRHKGMSLLVVERDMEGFERGRNLDKIGLHAQDTAELFFN
                     DVRVPAENLLGEEGTGFLQLVANLPQERLGIAAAGIAHGRAAFDQTLHYTKERTAFGQ
                     PIGSFQNSRFKLAEMATELEIGQTFVDRCVMENNAGTLTAETASMAKWWCTELQKRVM
                     DACLQLHGG"
     gene            1147..2295
                     /locus_tag="PLP95_08660"
     CDS             1147..2295
                     /locus_tag="PLP95_08660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012226864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HPB45908.1"
                     /translation="MPQASEDHELFRSTVREFVEREINPFAEEWEETGVFPARELFSK
                     MGSLGFLGLTYDPAYGGGGADYGYVCILNEELGRMLPLGVAMAIGVQTDMATPSLHRF
                     GSHELKEQYLRPAITGEAVAAVAITEPDAGSDVAGIRTKAHLEGGEWVLNGAKTFITN
                     GTQADWVCVVARTSDEGGSRGVSQILVPTDAPGFIVSRKLDKLGMRSSDTAELVFENC
                     RVPAANLIGSEGNGFKQQMQQFQDERMVASHQMAGACHYALERTGAYLRERMAFGKPL
                     MANQHLAYELAELSAEVDMLRVYNWEAAMAQSRGEDITRYATIAKLKSGRLARRVADV
                     CLQYHGGLGYMEENWTARFFRDTRLWSIGGGADEIMLRTLARVDGFWA"
     regulatory      2340..2450
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            2439..>3035
                     /locus_tag="PLP95_08665"
     CDS             2439..>3035
                     /locus_tag="PLP95_08665"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004788 - thiamine diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0006772 - thiamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HPB45909.1"
                     /translation="MTIGVVLAGGASTHQHVPGWLHDPSVACLVVAADSGLDLAQRLG
                     VDVDLLVGDLDSVSASALGRARRDGVEILEYPSDKDATDLELALGIAIEELGRDHGSG
                     RDHGSVGESNRIVVLGGAGGRLDHLLGNVALLASMSTLHGVAIEAYLGGAYVAVVDSA
                     LQPRWSATVRSGAMLSVMAWSPEAVVSESGVRWTLRSHR"
BASE COUNT          472 a          968 c         1060 g          535 t
ORIGIN      
        1 ccgccgtgca gttgcaggca cgcatccatc acccgcttct gcagttcggt gcaccaccac
       61 ttggccatcg atgcggtctc ggcggtgagg gttccggcgt tgttctccat cacgcaacgg
      121 tcgacgaagg tttggccgat ctcgagttcg gtcgccatct cggccagctt gaagcggctg
      181 ttttggaacg agccgatcgg ctggccgaac gcggtgcgct ccttggtgta atgcagcgtc
      241 tggtcgaacg cggcccgacc gtgggcgatc cccgcggcgg cgatccccag gcgttcctgc
      301 ggcaggttgg cgaccaactg caggaacccg gttccctcct cgccgagcag gttctctgcg
      361 gggacacgga cgtcgttgaa gaacaactcg gcggtgtcct gggcgtgcag gccgatcttg
      421 tcgaggttgc ggccgcgttc gaatccttcc atgtcgcgtt cgaccaccag caggctcatt
      481 cccttgtggc gttgggtggg gtcggtcttg gcggcgacga tgacgaggtc ggagttgatg
      541 ccgttggtga tgaacgtctt ggagccgttg aggaggtagg tgtcgccgtc gcggatcgcg
      601 gtggtctgca tgccggccag gtcggacccc atggcgggct cggtcatcgc gatcgcggtg
      661 atcagggttc cgttcgccat gcccggcagc caccgctgct tttgttcgtc gttgcagtag
      721 cgcaggaagt acgggagcgc gacgtcgcta tgcaacgtga agccggcgcc ggtggccatc
      781 gccccggccc gggccagttc ctcgacgacc accaggttgt agcggaaatc cttgaccccg
      841 gggccgccgt actcctcggg ggtgtcgaac gcgaggaatc ccgcggctcc ggcctcctcc
      901 cacacgtcgc ggctgacgat gcccgccgcc tcccattcct cgcggcgcgg cacgagggac
      961 cgctcgacga aggaacggta ggcctcgcgg aacatttcgt ggtcatcggt gaagatcggt
     1021 cgtgtcatcg cgacagcctg ccatgagacc gggtctgggc catacgatgg tgcacgttca
     1081 ttcgagcgct ggggccgacg ctccctgcgc caccacgcgt cgtcaagaca cccgacagga
     1141 gcaccgatgc cccaagccag cgaggaccac gaactgttcc gatcgaccgt gcgggaattc
     1201 gtcgaacgcg agatcaaccc gttcgccgag gagtgggagg agaccggggt cttcccggcc
     1261 cgtgagttgt tctcgaagat gggctcgctc gggttcctcg ggttgaccta cgacccggcc
     1321 tacggcggcg gtggtgccga ctacggctat gtgtgcatcc tcaacgagga acttgggcgc
     1381 atgttgcccc tcggcgtggc gatggcgatc ggcgtgcaga ccgacatggc cacgccgtcg
     1441 ctgcatcgtt tcggcagcca cgaactcaaa gagcagtacc tgcgcccggc catcaccggc
     1501 gaggcggtgg cggcggtggc gatcaccgaa cccgacgccg gctccgatgt cgcaggcatt
     1561 cgcaccaagg cccatctcga gggcggcgaa tgggtgttga acggtgccaa gacgttcatc
     1621 accaacggca cccaggcgga ttgggtgtgc gtcgtggccc gcacctccga cgagggggga
     1681 tcgcggggcg tttcacagat cctcgtgccc accgacgccc ccgggttcat cgtgagtcgc
     1741 aagctcgaca agttggggat gcgcagttcc gacaccgccg agttggtctt cgagaactgt
     1801 cgggtgcccg ccgccaacct gatcggatcc gagggcaacg ggttcaaaca acagatgcaa
     1861 cagttccaag acgagcgcat ggtggcctcg catcagatgg cgggcgcatg tcactacgcg
     1921 ctcgaacgca ccggcgccta cctccgcgag cgcatggcgt tcggcaagcc cctcatggcg
     1981 aatcagcacc tggcctacga gctggccgag ttgtcggccg aggtcgacat gttgcgggtc
     2041 tacaactggg aggccgcgat ggcccagagc cgcggcgagg acatcacccg ctacgccacg
     2101 atcgccaagc tgaagtcggg ccgcctcgcc cggcgggttg ccgacgtgtg cctgcagtac
     2161 cacggtggcc tcggctacat ggaggagaac tggacggccc ggttcttccg cgacacccgg
     2221 ctgtggtcga tcggcggagg cgcggacgag atcatgttgc gtaccctcgc ccgggtcgac
     2281 ggcttctggg cgtgagttcg acgggagcct gcggcgccgt gtgctagaac ggcatccgac
     2341 aaccgaacac ggggagcttg ggcatccagg ctgagagggc aacatgtgcg tcgccgaccc
     2401 gggaacctga tctgggtaat gccagcggag gaatcgtcat gaccatcggg gtcgtcctgg
     2461 caggcggcgc gtcaacccat cagcacgtgc cgggttggct ccacgatccg tccgtggcat
     2521 gcctggtcgt cgccgccgac agcggcctcg acctggcgca gcggcttggg gtggacgtgg
     2581 acctcctcgt cggcgacctc gattcggtga gcgcctcggc gctggggcgg gctcgacgcg
     2641 acggtgtcga gattctcgag tacccctccg acaaggacgc caccgacctc gaactcgcgc
     2701 tcggcatcgc gatcgaggaa ctgggccgtg accacgggtc gggccgtgac cacgggtcgg
     2761 tcggcgagtc gaatcgcatc gtcgtcctgg gtggggcggg tggtcggctc gaccatctgc
     2821 tcggcaatgt ggcgttgctc gcgtcgatgt ccacgctgca tggggtggcg atcgaggcgt
     2881 acctcggcgg cgcgtatgtc gccgtggtcg actcggcgct acagccgagg tggtcggcca
     2941 cggtgcgctc gggcgccatg ctcagcgtga tggcgtggtc gcccgaggcg gtcgtgtccg
     3001 aatcgggcgt gcgttggacg ttgcgctcgc atcgg
//
LOCUS       DAOOMA010000307         3029 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_39432_length_3029_cov_3.609953, whole genome shotgun sequence.
ACCESSION   DAOOMA010000307 DAOOMA010000000
VERSION     DAOOMA010000307.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 3029)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3029)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3029
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39432_length_3029_cov_3.609953"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..282
                     /locus_tag="PLP95_08670"
     CDS             <1..282
                     /locus_tag="PLP95_08670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB45910.1"
                     /translation="EYLMQLVDLTMLPAPIAFATRPFHRFLTTGFLEPAFRKQMHLSW
                     SRADQRAFDLLFSSIGMFNRHLPTPVRAFPLNAYLYDFRQRVRSGKALV"
     gene            complement(245..1504)
                     /locus_tag="PLP95_08675"
     CDS             complement(245..1504)
                     /locus_tag="PLP95_08675"
                     /inference="COORDINATES: protein motif:HMM:NF017760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HPB45911.1"
                     /translation="MSVAATVPPVANTSTFASLRVANFRRFFIGQSISQVGNWLTVLA
                     QSLLILHLTGSGFAVGLLTAALCGPVLLFGPMAGLIADRVDKRKLLIGVQSAAMVQSL
                     LLALVAFSDDPSIPAIFVLAFMGGVLTALDNPARRSIVVEMVPVESVNNAVSINSALM
                     TGSRIIGPAIAGGLIATVGYGWCFLIDGISYIAVLIAFARMNPAEFRRAEAPAPGKGQ
                     VRAGLRYVRNEPVLWVPLVMATIIGTFTFNFSVVLPLLVVDSFGGTDTTFTTLLSVLS
                     IGSFVSALALGRRAVVPLRHVWVAAAAFGVSMLGLAAAPSMAIAYPIAVLIGASSIAF
                     MVSSTAIVQLNADPSMRGRVLALQAMVFLGTTPIGGPILGAICEWIDPRAGVALGAVA
                     ALVAAAYGWSRDPDRITPRLYRSAPAA"
     gene            complement(1501..2079)
                     /locus_tag="PLP95_08680"
     CDS             complement(1501..2079)
                     /locus_tag="PLP95_08680"
                     /inference="COORDINATES: protein motif:HMM:NF024854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HPB45912.1"
                     /translation="MSDSRAAAADSTTGTDVAAAPSGMGDLDEVAASIRLAVLRLGRI
                     LRHQDPHELSAPLTTALLTLGRRGPMSLSELAAAERITSPTTSKIVDKLEGLGYVTKV
                     ADAHDRRVCRIAVTPQGAAEVDLIGSRRTAYVRRSIDALAPELQAALPDAVRVLEALV
                     SAAGTAAATNADETAADETAAGGLAAAGEVRR"
     gene            complement(2167..2844)
                     /locus_tag="PLP95_08685"
     CDS             complement(2167..2844)
                     /locus_tag="PLP95_08685"
                     /inference="COORDINATES: protein motif:HMM:NF013306.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biliverdin-producing heme oxygenase"
                     /protein_id="HPB45913.1"
                     /translation="MSTIEDTVEAPLAFSEQIRQATWGDHEGAEHATYMDDLLAGKLT
                     LAQYAVLKEQLWFVYLEIEKAAERFADDPAAQPFASKEWMDKLRRLPALERDLDFMSP
                     GWRGTITPTPETAEYIARLKEKASDWAAGWIAHHYTRYMGDLSGGQMIARLAWKLYDI
                     SPEEGAEFYHFADVESPKGFRDAYREALDALPYGDEEKAAMVEEVREAYRLNTLMLTH
                     LANYMPK"
BASE COUNT          554 a         1018 c          960 g          497 t
ORIGIN      
        1 gagtacctga tgcaactcgt cgacctcacg atgctgcccg cacccatcgc gttcgccact
       61 cggccgttcc acaggttcct gaccaccgga ttcctcgaac ccgcgttccg caaacagatg
      121 cacctgtcat ggtcgcgtgc cgatcaacgg gctttcgacc tgctgttttc ctcgatcggg
      181 atgttcaacc gccacctccc cacacccgtt cgggcgtttc cgctcaacgc ctacctgtac
      241 gacttcaggc agcgggtgcg gagcggtaaa gccttggtgt gatccgatcc gggtcgcggc
      301 tccagccgta cgccgccgcg acgagcgcag cgacggcccc gagtgcaacc ccggcgcgag
      361 ggtcgatcca ttcgcagatc gcccccagga tcgggccgcc gatcggcgtg gtcccgagaa
      421 acaccatggc ctgcaacgcc aatacccggc cgcgcatcga cgggtcggcg ttgagctgga
      481 cgatcgcggt cgacgacacc atgaacgcga tgctcgaggc accgatgagc accgcgatcg
      541 ggtacgcgat cgccatcgat ggggccgccg ccaaccccag catcgacacg ccgaacgcag
      601 cggcggccac ccacacgtga cgcagcggca caaccgctcg tcggcctaag gcgagcgccg
      661 agacgaacga cccgatgctc aacaccgaca aaagcgtcgt gaacgtcgtg tcggtccctc
      721 cgaagctgtc gacgaccaac agcggcaaca ccacgctgaa gttgaacgtg aacgtgccga
      781 tgatcgtcgc catcaccagc ggaacccaca acacgggttc gttgcggaca tatcgcagtc
      841 cggcgcgcac ctgacctttg ccgggggccg gcgcctcggc gcgacggaac tccgccggat
      901 tcatccgagc gaaggcgatc agcacggcga tgtagctgat gccgtcgatc aggaaacacc
      961 atccgtagcc gaccgtcgcg atcaggccgc cggcgatcgc cgggccgatg attcgcgacc
     1021 cggtcatcag ggcgctgttg atcgacacgg cgttgttcac cgactcgacc ggcaccatct
     1081 ccacgacgat ggaccgacgg gccgggttgt cgagcgcggt gagaacccct cccatgaacg
     1141 cgagcacgaa gatcgccgga atcgatggat cgtcgctgaa cgcaacgagc gccaacagca
     1201 acgactgcac catcgctgcc gactgaacgc cgatgagcag tttgcgcttg tcgacccggt
     1261 cggcgatgag gccggccatc ggcccgaaca acagcacggg gccacacagc gctgcggtga
     1321 ggagccccac ggcgaacccg ctgccggtga gatgcaagat gagcagcgac tgggccagca
     1381 cggtcaacca gttgccgacc tgggagatgc tctggccgat gaagaaccgg cggaagttcg
     1441 ccacccgaag cgaggcgaag gtgctcgtgt tggcaactgg tggaaccgtg gcggcaacgc
     1501 tcatcgccgc acctccccgg ccgcggccaa cccgccggct gcggtctcgt cggctgcggt
     1561 ctcgtcggca ttcgtcgcag cagcggtgcc ggcggcggac accagcgcct cgagcacccg
     1621 gactgcgtcg ggcagagcgg cctgcaactc gggggcgagc gcgtcgatcg accgccggac
     1681 ataggcggtg cgccgagagc cgatcaggtc gacctccgcc gcgccctgcg gggtgacggc
     1741 gatacgacac acccgccgat cgtgcgcatc cgccaccttg gtcacataac cgagcccctc
     1801 gagcttgtcg acgatcttcg aggtggtcgg cgaggtgatc cgttctgcgg cggcgagttc
     1861 ggacagcgac atcgggccgc gacgaccgag cgtcaacaac gcggtcgtca gcggcgccga
     1921 caattcgtga gggtcttggt ggcgaagaat tcggcccagg cgaaggaccg ccaagcgaat
     1981 cgatgcggca acctcgtcga ggtcgcccat gccgctgggc gctgccgcga cgtcggtacc
     2041 cgttgtggaa tcggctgctg cggcgcgtga gtcactcatc gccgatcagc ataccgatat
     2101 ttagttagtt agtgaaacta atttgccgag acgagcgtca cacggccagc gtcctacccg
     2161 gcgaggtcac ttcggcatgt agttcgcaag gtgcgtcagc atgagcgtgt tgaggcggta
     2221 ggcctcgcga acctcttcga ccatcgcggc cttctcctcg tcgccgtagg gcaacgcgtc
     2281 gagcgcttcg cggtaggcgt cgcggaagcc cttcgggctc tcgacgtcgg cgaagtgata
     2341 gaactccgca ccctcctcgg gcgagatgtc gtagagcttc caggcgaggc gggcgatcat
     2401 ctgcccaccc gacagatccc ccatgtaccg ggtgtagtgg tgggcaatcc atcccgctgc
     2461 ccaatccgaa gccttctcct ttaggcgagc gatgtattcg gcggtttccg gggtcggcgt
     2521 gatcgtcccg cgccagccgg ggctcatgaa gtcgaggtcg cgctcaaggg cgggaaggcg
     2581 gcggagcttg tccatccact ccttcgacgc gaacggctga gcggcggggt cgtcggcgaa
     2641 gcgctcggcg gccttctcga tttcgaggta cacgaaccag agttgctcct tgagcacggc
     2701 gtattgcgcc agcgtcagct tcccggcgag gagatcgtcc atgtaggtgg cgtgttccgc
     2761 cccttcgtga tcaccccagg tcgcttggcg gatctgctcc gagaaagcga ggggcgcttc
     2821 gacggtatcc tcaatcgtgc tcacgtgtaa ctccctgacg accggcgcct cagcgtcctc
     2881 cggccgtgtc gatcaatcgg catgcctgtt aggcgggctt cacactatac gattcgtccc
     2941 gattccaaca atggtgtgcc aaggcaccca caatttgtga gtagatgcat atgcccgagc
     3001 ccctcccgac cttcgacgat gccgtgatc
//
LOCUS       DAOOMA010000308         3023 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_39533_length_3023_cov_3.601415, whole genome shotgun sequence.
ACCESSION   DAOOMA010000308 DAOOMA010000000
VERSION     DAOOMA010000308.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 3023)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3023)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3023
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39533_length_3023_cov_3.601415"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..636)
                     /locus_tag="PLP95_08690"
     CDS             complement(<1..636)
                     /locus_tag="PLP95_08690"
                     /inference="COORDINATES: protein motif:HMM:NF019845.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HPB45914.1"
                     /translation="MRSVLIHEAGTVTVETVPDPSLGDARSAVIRVERSAICGSDLHF
                     YDGDLPMYPVAAGHEAVGTVIEVGAEVRSVGVGDRVLVASVAGCGRCAGCGTGNPTAC
                     VDGARVFGAGELGGAQSEYLAVPGADFNLLAIPEGIDDEAALLLTDNLSTGWTAAKRA
                     AFAPGADVLVLGLGAVGLCAVRSAYALGAARVFAYDPVVGRRDRGQRFGAIP"
     gene            714..2747
                     /locus_tag="PLP95_08695"
     CDS             714..2747
                     /locus_tag="PLP95_08695"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HPB45915.1"
                     /translation="MSDTNDSSGRSNFDDTTGSPTSGGSTSGEGLIAQPIAEVSEEQL
                     RSFLDDLSVPTLLVTCVHLAADDATRRNILDGPLRPAGSYLNEYQGFMSDEDQVAARQ
                     LALEIIADWRDRGAPTPEPISPELLKELMEWIVGMPVGDEYVPMMTEELALDGRDDRA
                     EEPTPGAQDLPVVVIGCGMSGLLAAIRLGQAGHPYVVLEKNDGPGGTWWENRYPGARV
                     DVGSHFYCYSFEPSDHWSEFFARQPELQAYFERVMDNHGVRPHVRFGVEATDADWDEE
                     RGVWVVSTSSGEIVEGRALLSAVGMLNRPFVPEVEGDFDGPAFHTARWRDDVDLTDRD
                     VVMIGAGATGFQVAPAIAEQVRSLTVLQRSAQWMFPNLGYHDAVGDGVRWAIANLPYY
                     GRWFRFLIFFPGCDTGLDAAIVDPDYVDAEGGQRLAVSEINEMARGFFADWIIGQVDG
                     DPDLVAKVVPDYPPTGKRTLQDNGSWLRTLTRGNVELVRAGVERLEPDGVVDSDGVKH
                     RADVVVWATGFCANDLISPLRIRGRGGVDLHDAWARRPRALLGITVPEFPNFFMLYGP
                     GTNLASGGSIIFSSECQVRYVMGCLRLLAEQHGTSIEPTTAAYEDWFDRCQERVRQTV
                     WASPHIAHSYYKADDGEVYVLNPWRLVDYWTWTREPNATDYVVSAATGRGAST"
     gene            2757..>3023
                     /locus_tag="PLP95_08700"
     CDS             2757..>3023
                     /locus_tag="PLP95_08700"
                     /inference="COORDINATES: protein motif:HMM:NF022182.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjN domain-containing protein"
                     /protein_id="HPB45916.1"
                     /translation="MGDTENDAIDLEHANGWLAFEMLGQLLDQEEFHPEQMSETTAYR
                     FGVAGTNGNFRVVVHCNVELEQLYVYVLTDVRVPEDRRLAVAELV"
BASE COUNT          511 a          998 c         1009 g          505 t
ORIGIN      
        1 ggggatcgcg ccgaaacgct gcccccggtc gcggcggccg accaccgggt cgtaggcgaa
       61 gacccgggcc gcgccgagcg cgtacgccga gcgcacggcg cacaggccta cggctcccag
      121 ccccagcacc aagacgtcgg ctcctggcgc gaaggctgct cgtttggccg cggtccagcc
      181 ggtgctgagg ttgtcggtca acaacagggc cgcctcgtcg tcgatacctt cggggatcgc
      241 caggaggttg aaatccgcgc cgggcaccgc gaggtactcg ctttgggcac cgcccagctc
      301 tccggcaccg aacaccctcg ctccgtcgac acacgccgtc gggttgccgg tgccgcatcc
      361 ggcgcagcgc ccgcatcccg caaccgacgc cacgagcacc cggtcgccca cgccgaccga
      421 acgaacctcg gctcccacct cgatcacggt gccgacggct tcgtgtcccg cggcgacggg
      481 atacatcggc aggtcgccgt cgtagaagtg caggtcgctt ccgcagatcg ccgatcgttc
      541 gacccggatg acggcgctgc gggcatcgcc aagcgagggg tcgggaacgg tttcgaccgt
      601 taccgtgcca gcttcatgga taaggaccga tcgcatggct gcaccatagg tcgcgcgctc
      661 ccgggcagga gcgggagcgg gttgtatcga cgcgggtgcc tacactcgcc gcggtgagcg
      721 acaccaacga ctccagcggt cgcagcaact ttgacgacac cacgggcagc ccgacctctg
      781 gcggctcgac ctccggcgag ggactcattg cccaacccat cgccgaggtc tccgaggaac
      841 agcttcggtc gtttctcgac gatctgtcgg ttccgacgct gttggtgacc tgcgtacacc
      901 tggccgccga cgacgcgaca cgtcggaaca ttctcgacgg cccgctgcga cccgccggta
      961 gctatctcaa cgaataccag gggttcatgt cggatgagga tcaggtcgcg gcgcgccaac
     1021 tcgccttgga gatcatcgcc gactggcgag accgaggcgc cccgacgccc gaaccgatat
     1081 cgcccgaact gctcaaggag ttgatggagt ggatcgtcgg aatgccggtc ggcgacgagt
     1141 atgtgccgat gatgaccgag gagctggccc tcgacggtcg cgacgaccgc gctgaggaac
     1201 ccacccctgg cgcgcaagac ctgcccgtcg tggtgatcgg ttgtgggatg agcggtctgt
     1261 tggcggcgat tcgcctcgga caggccggtc acccctatgt cgtgctggag aagaacgacg
     1321 gcccgggcgg aacgtggtgg gagaaccgct atcccggtgc ccgcgtcgat gtcggaagtc
     1381 acttctattg ctacagcttc gagccgagcg atcactggtc ggagtttttc gcccgccaac
     1441 cagagctgca ggcctatttc gagcgggtga tggacaacca cggggtccgg ccgcacgtgc
     1501 gattcggcgt cgaggccacc gatgcggact gggatgagga acgcggcgtg tgggtggtgt
     1561 cgacgtcctc gggtgagatc gtcgaggggc gcgcgttgct cagcgcggtg ggcatgttga
     1621 accgcccctt cgttcccgag gtggaaggcg acttcgacgg tcccgcgttc cacaccgccc
     1681 ggtggcgtga cgacgtcgac ctcaccgatc gcgacgtcgt gatgatcggc gcgggtgcca
     1741 ccggattcca ggtggccccg gcgatcgccg agcaggtccg ctcgctcacc gtgctgcaac
     1801 gcagcgcgca gtggatgttc cccaacctcg gctatcacga tgccgtcggc gacggagtgc
     1861 ggtgggccat tgccaacctg ccgtattacg ggcgttggtt tcgtttcctc atcttcttcc
     1921 ccgggtgcga taccggtctc gacgcggcga tcgtcgaccc tgactacgtc gacgccgaag
     1981 gcgggcaacg tctggcggtc agcgagatca acgagatggc gcgagggttc tttgccgact
     2041 ggatcatcgg ccaggtcgac ggcgaccccg atctggtcgc caaggtcgtt cccgactacc
     2101 cgccgacggg caaacgcacc ctgcaggaca acgggagttg gctgcgtacc ctgacccgcg
     2161 gcaatgtcga actcgtccgc gccggggtcg aacgcctgga gcccgacggg gtggtcgatt
     2221 ccgatggggt caagcaccgg gccgatgtcg tcgtgtgggc caccgggttc tgcgcgaacg
     2281 acctcatcag cccgctgcgc atccgtggac gcggtggggt ggacctacat gacgcctggg
     2341 cgcgacgccc tcgggcgctg ctgggcatca ccgtgccgga gtttcccaac ttcttcatgc
     2401 tgtacggacc cggaaccaac ctggcgagcg gcggcagcat catcttcagc agcgaatgtc
     2461 aggtgcgcta tgtgatgggg tgcctgcgac tgttggccga gcaacacggc acctctatcg
     2521 agcctacgac agcggcctac gaggactggt tcgaccgctg tcaggagcgg gtccgccaaa
     2581 cggtgtgggc ctcaccacac atcgcccaca gctactacaa ggccgatgac ggcgaggtgt
     2641 acgtgctcaa cccgtggcgc ctcgtggact actggacctg gacccgcgag cccaatgcga
     2701 cggactatgt cgtgtctgca gcgacggggc gtggcgcctc gacgtagcct gtgtacatgg
     2761 gcgacaccga gaacgacgcc atcgacctcg aacatgccaa cggttggctg gccttcgaga
     2821 tgctgggcca gctcctcgac caggaggagt tccacccgga gcagatgagt gaaacgacgg
     2881 cgtaccgatt cggcgtcgcc ggcaccaacg gcaacttccg agtggtcgtt cactgcaatg
     2941 tggaactcga acaactctac gtctacgtgc tcaccgacgt tcgggttccc gaggaccgac
     3001 gtctcgcggt tgcggagttg gtc
//
LOCUS       DAOOMA010000309         3021 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_39571_length_3021_cov_3.481119, whole genome shotgun sequence.
ACCESSION   DAOOMA010000309 DAOOMA010000000
VERSION     DAOOMA010000309.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 3021)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3021)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3021
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39571_length_3021_cov_3.481119"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..199
                     /locus_tag="PLP95_08705"
     CDS             <1..199
                     /locus_tag="PLP95_08705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB45917.1"
                     /translation="LAGAGLDTFDPEPWDATPSIARHPGVVVTPHIAWLTWETLERSL
                     DLGVAAALAVRDGTDIANRVV"
     gene            221..637
                     /gene="arfB"
                     /locus_tag="PLP95_08710"
     CDS             221..637
                     /gene="arfB"
                     /locus_tag="PLP95_08710"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: protein motif:HMM:NF006718.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome rescue aminoacyl-tRNA
                     hydrolase ArfB"
                     /protein_id="HPB45918.1"
                     /translation="MAEPGEDLIIGRAVRVSASEMTWRFSASGGPGGQHVNTSNTRVE
                     LSVDLRTVLANDTVRHRLLKRYGHTLVVVASEHRSQYQNRRVALAKLEQHLRRGLVVE
                     RSRVATKPTRGSQMRRLEGKKRRSAIKADRRRPPSD"
     gene            705..1676
                     /locus_tag="PLP95_08715"
     CDS             705..1676
                     /locus_tag="PLP95_08715"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012230642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HPB45919.1"
                     /translation="MQRHGITIPFDSVPLAEQRDWIAEVEQLGYTDVWSAEADATDAF
                     TPLVLASQWAPTLRLGTAIVPAYTRAPGVLAQQIATLAAVAPGRVAFGVGASSNVIVE
                     RWNGVPFEDPYRRVRDTVRFLRPTLAGEKTDFDGSNLSSHGFRLKIKGLDTKVPILVA
                     ALREGMLRLAGREGDGAIINWLSANDVRQVAPIVQEASPDGSHKEIAARIFVVPSTDR
                     ELVMNYSKFAIAAYLNVAVYAAFHEWLGRGEMLAPMWKHWREGDRAAAVAAIPDELVD
                     ELVVWGSPEACREHIGRYVEAGVDTTVLAILNFGIDERQAIRDLAPR"
     gene            complement(1703..2464)
                     /locus_tag="PLP95_08720"
     CDS             complement(1703..2464)
                     /locus_tag="PLP95_08720"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF015146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008263 - pyrimidine-specific mismatch
                     base pair DNA N-glycosylase activity [Evidence IEA];
                     GO_process: GO:0006285 - base-excision repair, AP site
                     formation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mismatch-specific DNA-glycosylase"
                     /protein_id="HPB45920.1"
                     /translation="MSRASRDSAQRDNPSDNASVEGADLADILAGGPRTEVPIELWRL
                     RAATPVGHRFHVPADVAQRWPTDLLAATLEGGAFNPSEGVYVRARTLADLVGADLRVL
                     IVGLNPSLVAADAGVGFAGATNRFWPAALDAGLVSVDRDPLHAFEHHRIGMSDLVKRA
                     TVSAKELSTAQYRHGIRRLATTVRWLRPRAVCVVGITGWRAARDRSATIGWQDEGFGT
                     TPVYVMPNPSGLNAHVSRAELAEHFHTVASATHRA"
     gene            complement(2461..>3021)
                     /locus_tag="PLP95_08725"
     CDS             complement(2461..>3021)
                     /locus_tag="PLP95_08725"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HPB45921.1"
                     /translation="LDLYCSNAGIGTGMGIDATDDLWHRMFDVNVMGTVNAARAFLPV
                     VRSQGRGHLLVTASAAGLLTNLGDAAYSATKHAAVGLAEWLSITHADEGLTVSCLCPQ
                     GVNTPLVTGGIGGPGGATLATDVVKLMGLIEPDDVADAVVEGLAEDRFLILPHPEVAD
                     YVRTKADNVDRWLDAMRKLQRRLLAT"
BASE COUNT          472 a          963 c         1076 g          510 t
ORIGIN      
        1 tctcgccggt gcgggtctcg acacgttcga tcccgagccg tgggatgcca ccccgtcgat
       61 cgcgagacac cccggtgtcg tcgtgacccc gcacatcgcg tggctgacct gggagacctt
      121 ggagcggtcg ctcgatctgg gggtggccgc ggcgctggcg gtgcgcgacg gcacagatat
      181 tgccaaccgc gtcgtgtgat gtcgctcgta acatgacgtc gtggccgaac ccggcgagga
      241 cctgatcatc ggacgtgccg tgcgcgtcag cgcgtcggag atgacgtggc gcttcagcgc
      301 gtccggcggc cccggcggcc agcatgtcaa cacctccaac acccgcgtcg agttgtccgt
      361 cgacctgagg acggtcctgg ccaacgacac ggtccgtcac cggctcctca agcgctatgg
      421 ccacaccctc gtggtggtcg cgagcgagca ccgctctcag tatcagaacc gtcgcgtcgc
      481 gctcgcgaag cttgagcagc atctgcgtcg tggtctggtc gtggagcggt cgcgggtggc
      541 gaccaagccg acgcggggct cccagatgcg ccgtctcgag ggcaagaagc gccgttcggc
      601 gatcaaggcc gaccgtcgcc ggccgccttc ggactgagcg cctgccgccc tcggactgag
      661 cgtccgccgc tcatcgccgc ggcatcgcgc gatagcgtcc gcacatgcag cgccacggca
      721 tcaccattcc cttcgacagt gtcccgctcg ccgaacaacg cgattggatc gccgaggtcg
      781 aacagctcgg atacaccgac gtgtggtccg cggaggcgga cgccaccgat gccttcactc
      841 cactggtcct ggcgtctcag tgggcgccga cgttgcggct cggcaccgcc atcgtgccgg
      901 cctacacgcg ggcaccgggt gtgctcgccc agcagattgc gacgttggcg gcggtggctc
      961 cgggtcgtgt cgcgttcggg gtcggtgcct catcgaacgt catcgtcgaa cgttggaacg
     1021 gcgtgccatt cgaagacccg tatcgacggg tgcgagacac cgtgcggttc ctgcgaccga
     1081 cgttggccgg cgaaaagacg gacttcgacg ggtcgaacct gtcgagtcac gggttccggc
     1141 tcaagatcaa gggactcgat acgaaggtgc cgatcctcgt cgctgcattg cgggagggga
     1201 tgctgcgtct cgccgggcgt gaaggcgacg gcgccatcat caactggctg agtgcgaacg
     1261 acgtccgtca ggtggctccc atcgtgcagg aggcgtctcc ggacggttcc cataaggaga
     1321 tagcggcccg gatcttcgtc gttccctcga ccgatcggga attggtcatg aactactcga
     1381 agttcgcgat cgccgcgtat ctcaacgttg cggtgtacgc ggcgtttcac gaatggctcg
     1441 gacgtggcga gatgctggct ccgatgtgga agcactggcg cgaaggagat cgagctgcgg
     1501 cggtggcggc gatcccggat gaactcgtcg acgagctcgt cgtgtggggc agccccgagg
     1561 cgtgccgcga acacatcggc cgctatgtcg aggcgggggt cgacaccacg gtgctggcga
     1621 tcctgaactt cggtatcgac gaacggcaag cgattcggga tctggcgccg cgctgagggc
     1681 agagcgcgga agtcgtcgtc gttcaggcgc ggtgggtggc ggaggcgacg gtatggaagt
     1741 gttcggcgag ttcggcgcgg ctgacgtgtg cgttgagccc cgaggggttg ggcatgacat
     1801 agacgggcgt ggtgccgaac ccctcgtctt gccagccgat ggtcgccgat cggtcccggg
     1861 ccgcacgcca gccggtgatc ccgaccacgc atacggcgcg agggcgtagc caccgcaccg
     1921 tggttgcgag tcgacggatt ccgtggcggt attgcgctgt cgacagttcc ttggcggaga
     1981 ccgtcgcccg tttcaccagg tcgctcatgc cgatgcggtg atgctcgaag gcgtgcaacg
     2041 ggtcccggtc gacactcacg aggccggcgt cgagcgccgc gggccagaac cgattggtcg
     2101 ctccggcgaa ccccactccc gcatccgcgg cgacgaggct cgggttgagg ccgacgatga
     2161 gcactctgag gtcggcaccg acgagatcgg cgagtgtccg agcgcgcacg tagacgccct
     2221 cggacgggtt gaacgcccct ccttcgaggg ttgccgcgag caggtcggta ggccaccgtt
     2281 gggcgacgtc ggcggggacg tggaatcggt gcccaacggg tgtcgccgca cgaagtcgcc
     2341 agagttcgat cggcacctcc gtgcgaggcc cgccggcgag gatgtcggcg aggtccgcgc
     2401 cctcgactga ggcgttgtcc gaggggttgt cacgctgggc gctgtcacgc gaagcccgac
     2461 tcatgtggcg agaaggcggc gttgcagctt gcgcattgca tcgagccatc ggtcgacgtt
     2521 gtcggccttg gtgcgcacat agtccgcaac ctcggggtgg ggcaggatca ggaatcggtc
     2581 ctccgctagt ccctccacga cggcgtcggc gacgtcgtca ggctcgatga ggcccatgag
     2641 tttcaccacg tcggtggcga gggtcgcacc acctgggccg ccgatgccgc cggtcaccag
     2701 cggggtgttg accccctgtg ggcacaggca cgaaaccgtc aacccctcgt ctgcgtgcgt
     2761 gatcgacaac cattcggcga ggcccaccgc agcgtgtttg gtggcgctgt aggcggcgtc
     2821 gccgaggttg gtgagcaatc cggccgccga cgcggtcacg agcaagtggc cgcggccctg
     2881 gctacgcacg acgggaagga aggctcgagc cgcgttgacg gtgcccatga cgttgacgtc
     2941 gaacatccga tgccacaggt cgtcggtggc gtcgatgccc atgccggtgc cgatcccggc
     3001 gttggagcag tacaggtcga g
//
LOCUS       DAOOMA010000310         3020 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_39588_length_3020_cov_4.254975, whole genome shotgun sequence.
ACCESSION   DAOOMA010000310 DAOOMA010000000
VERSION     DAOOMA010000310.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 3020)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3020)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3020
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39588_length_3020_cov_4.254975"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1151
                     /locus_tag="PLP95_08730"
     CDS             <1..1151
                     /locus_tag="PLP95_08730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB45922.1"
                     /translation="PVGQWDRYNIVPLVDGRGFLVAGGGNSVDGYERPRPHNLEAALF
                     RFSTGAYELLPELPVKAGLGAGSGAVVGGPDEETVVVVGQDCPPGPEVSAVGIEFCSW
                     GGSGAKVVLSWNTGEGSWTRHELPKKLEDFSPASPSVIGVDGGLVWLASETTGRVPRI
                     ASFDPSTGSFSEVIDGPAEQAGICLIGSDLLAFVTPATTFVSDMKVSSWAFDGAAWQE
                     LPSWGDVPTQFTPSEALCTPQGYYSVVPTSESFWSDLRWTESAEAAKHATFTEVGRFV
                     NGLAGWRIGIRQFGSDVLVGLSLQMPDLGSMMTTIPGDPPVDPSEMKTLPGNVTAGWN
                     LVTPTGPVPVPGLSPDEWYAAVTIGDHVLIEVTDASDDYDLLALRTPV"
     gene            complement(1224..2501)
                     /locus_tag="PLP95_08735"
     CDS             complement(1224..2501)
                     /locus_tag="PLP95_08735"
                     /EC_number="2.3.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012228855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046912 - acyltransferase activity, acyl
                     groups converted into alkyl on transfer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HPB45923.1"
                     /translation="MRHVSDSITITDNRTGEQVEVPIVNGAVDAKAWAKLLPGIWFLD
                     PSFGTTAATESAVTELDGEAGILRYRGYPIEQLAEHSSYLEVAYLLIYGELPTAEQFE
                     KWRYDIVHHTYIHENVRKRFMEGFHYDAHPMGMLVSSAAALSTFYPEAKNIEDPANLD
                     RQIVRLIAKMPTLAAGAYRFSVGMPFVYPDNSLDFTSNFLSMMWKIAEPRFDAHPVLS
                     RALDILFILHADHEQNCSTTAMRTVGSSHADPYSSTAAAAAALYGPRHGGANEAVLRM
                     LNEIGSVDNVASFIEAVKQGKGRLQGFGHRVYKNFDPRATIIKKTAYEVFEVTGKNPL
                     LDIALKLEEVALGDDYFVSRKLYPNVDFYSGLIYQAMGFPLDMFTVLFAIPRTSGWLA
                     HYKESLEQNSRISRPRQAYIGSEVRDYVPMTQR"
     gene            complement(2665..>3020)
                     /locus_tag="PLP95_08740"
     CDS             complement(2665..>3020)
                     /locus_tag="PLP95_08740"
                     /inference="COORDINATES: protein motif:HMM:NF015431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HPB45924.1"
                     /translation="VLISAVGTSFRSELDEAAERVGVRFISKAEIDGMRLLASLAFSG
                     YGAAVLPASAVPGTLDGAWRRVMISDISTRSVGLARRRRGLLSPAERAVAEVIREVVA
                     DEVPQQPGLALTGTP"
BASE COUNT          493 a          944 c         1037 g          546 t
ORIGIN      
        1 cgccggtcgg ccagtgggat cgctacaaca tcgttccgct cgtcgatggc cgcggattcc
       61 tcgtggccgg gggtgggaac agcgtcgacg gatatgagcg gccgcgcccg cacaacctcg
      121 aggcggcgct gtttcgcttc tccaccggcg cctatgaact gctgcccgag ttgccggtga
      181 aagccgggct cggcgcgggc agcggcgcag tggtcggcgg accggacgag gagaccgtgg
      241 tcgtcgtcgg acaggattgc ccgcccggtc ccgaggtttc cgccgtcggc atcgagttct
      301 gcagctgggg tggctccggg gcgaaggtcg tgttgtcctg gaacaccggc gaggggtcgt
      361 ggacgcgcca cgagctaccc aagaaactcg aggacttttc gcccgcgagt ccgtcggtca
      421 tcggtgtcga cggcggactc gtatggctgg cgagcgagac caccggccgc gtcccacgga
      481 tcgcgtcctt cgatccgtcg accgggtcgt tctccgaggt gatcgacggc ccggcggagc
      541 aagccggcat ctgcctcatc ggttccgatc tgctcgcgtt cgtgacccca gcgaccacct
      601 tcgtctccga catgaaggta tcgagttggg cattcgacgg cgcggcctgg caggagctgc
      661 cgtcgtgggg tgacgtcccg acccagttca ccccgtcgga ggcgttgtgt actccgcagg
      721 ggtactactc ggtggttccg acctcggagt cgttctggtc agacctccgg tggaccgagt
      781 cggccgaggc ggcgaaacac gcaacgttca ccgaggtcgg gcggttcgtg aacggtctcg
      841 ccgggtggcg catagggatc cgacagttcg gctccgacgt gctcgtgggg ctctccctgc
      901 agatgccaga cctcgggtcg atgatgacga cgattccggg tgacccgccg gtcgatccgt
      961 ccgagatgaa aacgctgccc gggaacgtga cggcgggatg gaacctcgtg accccgaccg
     1021 gaccggttcc tgtgccaggc ttatcccccg acgagtggta cgcggcggtc accatcggcg
     1081 accacgtcct catcgaggtg accgacgcgt ccgacgacta tgacctgctg gcattgcgca
     1141 cgccggtttg acgcagcgca cgccggtttg acgcagcgca cgccggtttg acgcagcgca
     1201 cgccggattg acggcgggct cgatcagcgc tgcgtcatcg ggacgtagtc gcgcacctcg
     1261 gagccgatgt aggcctgacg cgggcgagag attcggctgt tctgctcgag gctctccttg
     1321 tagtgcgcca gccagcccga ggtgcggggg atggcgaaca acacggtgaa catgtcgagc
     1381 gggaaaccca tcgcctggta gatgaggcca gagtagaaat cgacgttcgg gtacagcttg
     1441 cggctcacga agtagtcgtc gccgagcgcc acctcttcga gcttgagcgc gatgtcgagc
     1501 aacgggttct tgccggtcac ctcgaagacc tcataggccg tcttcttgat gatcgtggcg
     1561 cggggatcga agttcttgta gacgcggtgc ccgaagccct gaagccggcc cttgccctgc
     1621 ttgaccgcct cgatgaacga agcgacgttg tcgaccgagc cgatctcgtt gagcatgcgc
     1681 agcacggcct cgttggctcc gccgtggcgg gggccgtaga gcgcggccgc ggcggctgcg
     1741 gtggaggagt agggatcggc gtgggacgag ccgaccgtgc gcatcgccgt ggtcgaacag
     1801 ttctgctcgt ggtcggcgtg gaggatgaac aagatgtcga gcgcacggct gagcaccggg
     1861 tgggcatcga agcggggttc ggcgatcttc cacatcattg acaggaagtt cgacgtgaaa
     1921 tcgaggctgt tgtcggggta gacgaacggc atcccgaccg agaaccggta ggcgccggcg
     1981 gccagggtcg gcatcttggc gatgagtcgc acgatctggc gatcgagatt cgccgggtct
     2041 tcgatgttct tcgcctctgg ataaaaggtc gacagcgctg cggccgagga caccaacatg
     2101 cccatcgggt gggcgtcgta gtggaagccc tccatgaacc gcttgcgcac attttcgtgg
     2161 atgtaggtgt ggtgcacgat gtcgtagcgc cacttctcga actgctctgc ggtgggcagt
     2221 tcgccgtaga tcaacaggta ggcgacctcg agatagctcg aatgttcggc gagctgttcg
     2281 atcgggtagc cccggtagcg gaggatgccc gcctcgccgt cgagttcggt gactgcactc
     2341 tcggtggccg ccgtcgttcc gaaggacggg tcgaggaacc agatccccgg aagaagcttc
     2401 gcccaggcct tcgcgtcgac ggctccgttg acgatgggga cctcgacctg ttccccagtg
     2461 cggttgtcgg tgatcgttat ggaatctgac acgtgcctca tcttacgtcc cgggcggcgg
     2521 cctcggggcc acctgcgtgc agcgcacgaa gtgttcatct gctcgtgacc tcgcgcccgc
     2581 cgcattcgtg ggcgatctcc acctgccgtt ctccgataac cagtggtcgt gatcacgacc
     2641 actggttatc ggagaacagg caggtcaggg ggtgccggtg agggcgagac ccggttgctg
     2701 gggtacctcg tcggcaacca cctcgcggat cacctcggca acggcgcgtt cggccgggct
     2761 cagcaatccc cgacggcgcc gcgccaaccc gaccgaacgg gtgctgatgt cggagatcat
     2821 cactcgacgc caggcgccat cgagtgttcc cggcaccgcc gacgcgggaa gcaccgcggc
     2881 gccgtagccg ctgaacgcca gcgatgccag cagtcgcatc ccgtcgatct ccgccttcga
     2941 gatgaatcgc acgccgaccc gttcggccgc ctcgtcgagt tcgctacgaa agctcgttcc
     3001 gaccgcggag atcaacacct
//
LOCUS       DAOOMA010000311         3016 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_39671_length_3016_cov_4.151638, whole genome shotgun sequence.
ACCESSION   DAOOMA010000311 DAOOMA010000000
VERSION     DAOOMA010000311.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 3016)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3016)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3016
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39671_length_3016_cov_4.151638"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..793)
                     /locus_tag="PLP95_08745"
     CDS             complement(<1..793)
                     /locus_tag="PLP95_08745"
                     /inference="COORDINATES: protein motif:HMM:NF037041.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB45925.1"
                     /translation="MSLDTSELIPTLGAHLREWLPSQAWFDARLNEIDELTVVRTEAL
                     RRSWPIVLWVPVEVVIDAAPSLVHMFVALAASVPASVPDRGVIGEVPTGGTPLMAYDA
                     LLDPVAARAVVERLSDTMTDEVPTRIFERPWVTNIELGSRFDLTLYRRVQLGKHPDVD
                     LAGALAAAGLSVTCPPAAVWRRGDTDLANLRRRSKRGDLGVSIAQASARELLRRRCQP
                     RENPLDVMDSFALLGRNLADMHVELAEQYGVTVGDGVFETLKVVDG"
     gene            complement(796..2403)
                     /locus_tag="PLP95_08750"
     CDS             complement(796..2403)
                     /locus_tag="PLP95_08750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB45926.1"
                     /translation="MSELPGSDVSTATSPTRSRRSGPVIVAVLAGVVALVAVIIAVVA
                     TNGEPARTAPRPTTTVAAPEEPPRTDDGGGPGKIAYATSAGDVMVANSDGTEPVKIGG
                     DAATNGEGLAPLAWRQPGSDAITYVRRDGALVVAPINGAAPTVLATDAVVPADASDRI
                     LGWDATGTFVIYLAEPTPGRVESRAISWATGDETASGGATLIAGDGSGEPINIAFGDP
                     VNRRTRSQVVSPLDPVVYLDSVDPDTGAPYTAALVVPGGQDLLSSPFQLDDVMFSPDG
                     RYVFAVSRSVRGVEQLVRMSLRDPTKSDLVFDNERVCAPSMSPDGRYLVFGAGPDCSQ
                     MWRLNSDGTDPTMLTSDVRGATFAAGTFSWSLDSEIVSHPLCQDVGTEVSCGGPYLDV
                     SVADGTVTQRAVAGNVVREHRALLRSVHVGIEITGAIEYSGKMQAAEKSLAEELEVNE
                     GEGSHIVVIDEADAARRFELDVVRATGSNFVSGVIRVVDSGFDESFSFIGQLTPYSLG
                     YAKLNGVWTRSESVPLTSGLVEITLER"
     gene            complement(2441..>3016)
                     /locus_tag="PLP95_08755"
     CDS             complement(2441..>3016)
                     /locus_tag="PLP95_08755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020378348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HPB45927.1"
                     /translation="PERVREIGGAFLAALNAIAALPQPTIAEISGFALGGGCELALAC
                     DFRIASTRARFGQPEILLGIIPGGGGTQRLARLVGPSIAKDLVFTGRQVDAAEAYRIG
                     LANEVVEPEELAGRTRELAGQLAAGAPNALAIAKRAIDTGLDGTLEAGLELEQDAFVE
                     VFATPDAAVGVTSFLATGPGHADFGEIDRNA"
BASE COUNT          509 a         1043 c         1010 g          454 t
ORIGIN      
        1 ccccgtcgac caccttgagc gtctcgaaga caccatcgcc gacggtgacc ccgtactgct
       61 cggccaactc gacgtgcatg tcggccagat tgcgacccaa caacgcgaac gaatccatca
      121 cgtcgagcgg gttctcacgg ggctgacatc ggcgacgcag caactcccgc gccgatgcct
      181 gtgcgatcga aacgccgaga tcaccgcgtt tggagcgacg ccgaaggttc gccagatccg
      241 tatcgccgcg ccgccacacc gccgcgggcg gacaggtcac cgacagccca gcggcggcca
      301 aggcgccggc cagatcgacg tcggggtgct tgccgagctg cacccgccga tacagggtca
      361 gatcgaaacg cgatccgagt tcgatgttgg taacccaggg tcgttcgaag atccgggtcg
      421 gcacctcatc ggtcatcgtg tcgctcaacc gctccaccac cgcacgcgca gcgacggggt
      481 cgagcaacgc gtcgtaggcc atcaacgggg ttccgccggt cggcacctcg ccgatgacac
      541 cgcggtcggg caccgacgcg ggaaccgatg ctgcaagcgc cacaaacatg tgcaccagcg
      601 acggcgcggc gtcgatgacc acctcgaccg ggacccacaa cacgatcggc cagctgcggc
      661 gcagcgcttc ggtccgcacg acggtgagtt cgtcgatctc gttgaggcgc gcgtcgaacc
      721 aggcctgcga aggcaaccat tcgcgcagat gtgcgccgag tgtggggatg agttcagagg
      781 tgtcgaggct cacagctatc gctcgagcgt aatctcgacg agccccgagg tgaggggaac
      841 cgattccgaa cgggtccaca ccccgttcaa tttggcgtag ccgaggctgt agggcgtgag
      901 ttggccgatg aaactgaacg actcgtcaaa gccggagtcg acaacgcgga tgacccccga
      961 cacgaagttg ctaccggtcg cccgaacgac gtcgagttcg aaccgccggg cggcatcggc
     1021 ctcgtcgatc acgacgatgt ggctcccttc gccctcgttc acctcgagtt cttcggcgag
     1081 cgacttctcg gcggcctgca tcttgccgga gtattcgatc gcaccggtga tctcgatgcc
     1141 gacatggaca ctgcgcagca gggcgcggtg ctcgcgcacg acgttgcccg ccaccgcccg
     1201 ctgggtgacg gtgccgtcgg ccacgctcac atcgaggtac ggtccgccgc aactcacctc
     1261 ggtgccgaca tcctggcaga gcggatggct cacgatctcc gagtcgaggc tccacgagaa
     1321 ggtccccgcc gcaaaggttg caccacgcac atcggaggtg agcatcgtcg gatcggttcc
     1381 gtcggagttg agcctccaca tctgcgaaca atcaggaccg gccccgaaca cgaggtagcg
     1441 accatcgggc gacatggacg gcgcacacac ccgctcgttg tcgaacacca ggtcggactt
     1501 ggtcgggtcg cgaagcgaca tgcgaacgag ctgctcgacc ccgcgaaccg accgggacac
     1561 cgcgaacacg tagcgcccgt cggggctgaa catcacatcg tcgagctgga acgggctcga
     1621 caacagatcc tgaccgccgg gcacgaccaa cgccgcggtg tagggcgcac cggtatcggg
     1681 gtcgactgaa tcgagataca ccaccgggtc gagcggcgac acgacctgcg agcgggtgcg
     1741 tcggttgacc ggatctccga aggcgatgtt gatcggttcg cccgaaccgt ccccggcgat
     1801 gagggtggcg ccaccggatg cggtctcatc gccggtcgcc cacgaaatgg cgcgggactc
     1861 gaccctgccg ggcgttggtt cggcgaggta gatcacgaac gtgccggtcg catcccagcc
     1921 caggatccga tccgatgcat cggcgggaac gacggcatcg gtggcgagca ccgtgggggc
     1981 ggccccgttg atcggggcga cgaccagtgc gccgtcgcgg cgtacgtagg tgatggcgtc
     2041 gcttcccggc tggcgccacg ccaacggggc caacccctcg ccgttcgtcg ccgcgtcgcc
     2101 gccgatcttg accggttcgg tgccgtcgct gttcgccacc atcacgtccc cggccgaggt
     2161 ggcgtaggcg atcttgcccg gtccgccgcc gtcatcggtg cgcggcggct cctcgggggc
     2221 ggcgaccgtg gtcgtcggcc gcggggccgt gcgcgccggc tcgccgttcg ttgcgacgac
     2281 ggcgatgatg accgcgacga gcgcgacaac tccggcgagg acggcgacga tgaccggtcc
     2341 gctccgacgc gatctcgttg ggctcgtcgc ggtggagacg tcgctccccg gcagttcgga
     2401 catggccatc agtatgggtg gtcgaacgcg acgggtgcca tcaagcgttt cggtcgatct
     2461 cgccgaagtc ggcgtgcccc ggccctgtcg ctaggaacga cgtcacgccg acggcggcat
     2521 cgggggtggc gaacacctcg acgaacgcgt cctgttccag ttcgaggccc gcctccaggg
     2581 tcccgtcgag gccggtgtcg atcgcccgct tggcgatcgc cagcgcgttt ggcgccccgg
     2641 cggcgagttg ccccgccaac tcgcgggtgc gcccagcgag ctcctcgggc tcgacgacct
     2701 cgttggccag gccgattcgg tatgcctcgg ccgcgtcgac ctgtcgcccc gtgaacacca
     2761 ggtccttggc gatcgagggt ccgaccagac gggccagccg ctgggttccc cctccaccgg
     2821 ggatgatccc caggaggatc tcgggctggc cgaaccgcgc cctggtcgac gcgatgcgaa
     2881 agtcgcacgc cagcgccagt tcacaaccgc cgccgagcgc gaacccggag atctcggcga
     2941 tcgtcggttg gggcagggcc gcgatcgcat tcaaggccgc caggaacgcc cctccgatct
     3001 cgcggacccg ctcggg
//
LOCUS       DAOOMA010000312         3016 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_39677_length_3016_cov_3.216143, whole genome shotgun sequence.
ACCESSION   DAOOMA010000312 DAOOMA010000000
VERSION     DAOOMA010000312.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 3016)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3016)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3016
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39677_length_3016_cov_3.216143"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            193..1548
                     /locus_tag="PLP95_08760"
     CDS             193..1548
                     /locus_tag="PLP95_08760"
                     /inference="COORDINATES: protein motif:HMM:NF012870.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HPB45928.1"
                     /translation="MHDRHRDIALFRYSLIREAADPALTPTERGRLVRYLAARDHLDP
                     HGERVRVGRSTLDRWIRAWRGGGFDALVPQARSAEPKTPAALLDLAVRLKLEVPARTA
                     AQVCRIIEADKGWAPSERTVQRHFARRGLNVRPDGQPLEVFGRFEADTTNVLWTGDAL
                     HGPVIGGRKVYLLAFIDDHSRLLVGYRWCHSEDTIRLEAALRAGLASRGIPGGIYVDN
                     GSAFASKPLLRACASLGVRLTHSTPRRPEGRGKIERFFRTVRDQFLVEITVSGPTDLD
                     EMNRLFSAWVEGVYHRQVHSETGEAPLDRFDTSIVRFPTAGELHEAFLWSETRVVSKV
                     ATVSLLGNHYQVDAALAGQRVELVFDPFDLTHIEVRLRDLPMGTAVPQHIGRHTHPAA
                     RPDPTQLAAPTATGIDYLRLIETERDRNLAASGGIDFHQLALPKEPIVQPRTTNKQDQ
                     P"
     gene            1545..2360
                     /locus_tag="PLP95_08765"
     CDS             1545..2360
                     /locus_tag="PLP95_08765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020379012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HPB45929.1"
                     /translation="MSIDRLRAHWGFTRMPFNKDLAPSMLATTTTHAEAVARISWCVT
                     EDALGVITGEVGAGKTVAARAALGTLDTSRHTIIYIGNPAIGARGIYTTIITTLGGTP
                     RFHKAALIPQAQDALATERDERGKKVVVVLDEAHLLSTDQLEELRLLLNADMDSRSPF
                     ACLLIGQPTLRRLIKLGMFAALDQRIALRYAMAGMNAHETSTYIAHHLNLAGRSDTLF
                     SDDAIELIHQVARGLPRAVNNLAVQALIAAYATGRNIVDESSTRTAITEVTAE"
BASE COUNT          594 a         1070 c          809 g          543 t
ORIGIN      
        1 cgcggatgcg gttgaggccg tcgggatggc gacgcgggcg tcggtggtga ggttcgggcc
       61 gggctccgcg tggagtcggg cggggcggtt gtgcggcggg gcgttgttat ccaacacgag
      121 ttgcctctgg ttgacaccga gctgagcggg cattgtcacg ggctctaccc ctgttgagtc
      181 ttggagggct ccatgcacga tcggcatcgc gatatcgcgt tgtttcgtta ctcgttgatt
      241 cgcgaggcag cggacccggc gttgacgccg accgaacgcg gccgtttggt ccgctatctc
      301 gctgctcgcg accatctcga cccgcacggt gaacgggtcc gggtgggccg ctcgacgttg
      361 gatcgttgga tccgggcgtg gcgtggcggc gggttcgacg cgttggttcc ccaggcgcgg
      421 tccgctgaac cgaaaacccc cgcagcgttg ttggatctcg cggttaggtt gaaacttgaa
      481 gtcccggcgc gcaccgcggc ccaggtatgt cggatcatcg aagctgacaa gggttgggcg
      541 ccatcggagc ggacggtgca acgccatttc gctcggcggg gtctcaacgt tcgtcccgac
      601 ggccaaccac ttgaggtatt cggccgtttc gaggccgaca ccaccaacgt gttatggacc
      661 ggcgacgcgt tgcacggccc cgtcatcggt ggccgcaagg tctacctgtt ggcgttcatc
      721 gatgaccaca gtcggctatt ggtcggctat cggtggtgtc acagcgaaga caccatccgc
      781 ctcgaagcag cgttgcgcgc cgggcttgcc agccgaggaa ttcccggggg gatctacgtc
      841 gataacggtt ccgcgttcgc gtcgaaacct ctgttgcgag cgtgcgcgtc gttgggagtc
      901 cgcctcaccc actcgacacc gcgccgcccc gaaggccgcg ggaaaatcga acggttcttc
      961 cggaccgttc gcgaccagtt cctcgtcgag atcaccgtga gcggccccac ggacctcgac
     1021 gaaatgaacc gcctgttttc ggcttgggtt gagggcgtct atcaccgtca agtccactcc
     1081 gaaaccggcg aagcaccttt ggaccgcttc gacacgagca tcgtgaggtt cccgaccgcg
     1141 ggcgagctcc acgaagcgtt cctgtggtct gaaacccggg tcgtttccaa agtcgcgacc
     1201 gtgtcgctgc tcggcaacca ctaccaagtc gacgcagcgc tcgcgggcca acgcgtcgag
     1261 ttggtctttg accccttcga cctcacccac atcgaggtcc gcctgcgcga cctgcccatg
     1321 ggcaccgcag tcccacaaca catcggccgc cacactcacc ccgccgctcg cccagaccca
     1381 acccaactcg cagcgcccac tgcgaccggg atcgactacc tgcgcctgat cgaaaccgaa
     1441 cgcgaccgca acctcgccgc ctcaggcggg atcgactttc accaactcgc cctacccaaa
     1501 gaacccatcg tccaaccccg aaccaccaac aaacaggacc aaccgtgagc atcgaccgtc
     1561 tccgagccca ctggggcttt acccgcatgc cgttcaacaa agacctcgcc ccctccatgc
     1621 tcgccacgac caccacacac gccgaagcgg tcgcacgaat cagctggtgt gtcaccgaag
     1681 acgccctcgg agttatcacc ggcgaagtcg gcgccggaaa gaccgtcgcc gcacgagccg
     1741 ctctcggcac cctcgacacg tcgcgccaca ccatcatcta catcggcaac cccgccatcg
     1801 gcgcccgagg catctacacc acgatcatca ccaccctcgg cggcacaccc cggttccaca
     1861 aagcagcgct catcccccaa gcccaagacg ccctcgccac cgaacgcgac gaacgaggca
     1921 agaaagtcgt cgtggtcctc gacgaagccc acctcctcag caccgaccaa ctcgaagaac
     1981 tccgcctact tctcaacgcc gacatggaca gccgatcacc attcgcgtgt ctcctcatcg
     2041 gccaacccac cctgcggcga ctcatcaagc tcggcatgtt cgccgctctc gatcaacgca
     2101 tcgcgctgcg atacgccatg gccggaatga acgcgcacga gaccagcacc tacatcgcgc
     2161 atcacctcaa cctcgccggg cgcagcgaca ccctcttcag cgacgacgcc atcgagctca
     2221 tccaccaagt cgcccgcggg ctaccccgag ccgtgaacaa cctcgccgtt caagcactca
     2281 tcgccgcgta cgccaccggc cgcaacatcg tcgacgaatc ctccacccgc accgccatca
     2341 ccgaagtcac cgccgaatag caacgacaca ccgaccacaa tcccacgcca ccacgaccac
     2401 caccacgaca ccacgaccac caatccatcc ccacacaccg tgtcgccgcc atactcaccc
     2461 agcctggcgg gcaacaccgg gttcgccgag accacgacgt ggtcggtccg ctggagatcg
     2521 accgccgcag ccacgcggcc gaacacgtcg gcgaagtcat aggtgttcgc atctctcgcc
     2581 gattcaacga tgttctcgat atcgatcaga acaatctgcc gtttcttcga cgttcccagc
     2641 tgcgttgccg ttgtgcactt catcttggtc cccctggtgt gattaccatt cgacgtacgg
     2701 aaaatccgtc cgttgaaact tgcaattcca acttttctgt cagcgttcac tgcattttcg
     2761 gtgatgaaaa atcacgaatg gccgagcaaa agctctgcca ttcaaagaat tctctccacg
     2821 aaatctctcg ctgaatcctt cgaaacgacg aattttcaat cgttcgaaaa agttattccg
     2881 gagttttatc cagagtcttt tttccgcttc tttttgattt gcggaaaatt tctgtaaccg
     2941 gatctactca ggatcgcgca cctttgcccg ccgaccccct ggcgagccgg ggtgttgtac
     3001 atggggggcg cgaaac
//
LOCUS       DAOOMA010000313         3005 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_39882_length_3005_cov_3.779322, whole genome shotgun sequence.
ACCESSION   DAOOMA010000313 DAOOMA010000000
VERSION     DAOOMA010000313.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 3005)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3005)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3005
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39882_length_3005_cov_3.779322"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            43..513
                     /locus_tag="PLP95_08770"
     CDS             43..513
                     /locus_tag="PLP95_08770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020376955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family deazaflavin-dependent
                     oxidoreductase"
                     /protein_id="HPB45930.1"
                     /translation="MAVKSPKPYTEAQVRRGDAVIKRMSKLTTALYRRTNGRIGGRFL
                     RGAPVCLLTTIGRVSGEARTTPLLYLADGDDIVLVASKGGFPGNPNWYLNLKADPEVT
                     IQIGSVAGDYTAAQVSPQDKAELWPRLVAMYQDFETYQARTDRDIPVIRCRRRP"
     gene            599..973
                     /locus_tag="PLP95_08775"
     CDS             599..973
                     /locus_tag="PLP95_08775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB45931.1"
                     /translation="MDHRTTARLIAAGRTLFGLLCLLRPRVLTGFDRSEPSAAALGWI
                     RLFGVRDVVLGAGTLASLNGDDGDTRWVKMSAAADTADAVTVVTVRDEVGVSFMAASL
                     ALAVPAAALGWRAAFGLGRSRG"
     gene            1015..1665
                     /locus_tag="PLP95_08780"
     CDS             1015..1665
                     /locus_tag="PLP95_08780"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HPB45932.1"
                     /translation="MSFKAKFSKLILKLWRWTPVGERPEFSCVITAGPHTANIDYVLM
                     MLMAWSRGMELSWMGKHTLFEKPILGKFFTATGGIPVDRTAPQGLVGQMVERFEQTPR
                     LYLAIPPEGTRSLTEYWKSGFYRIAQGAGVPIVMCSIDTKTRVVEIGPTVHPSGDLVA
                     DMDLFREYYGPKVGFKPELYGPIRLREEEALGDGDALGDGNADGANPGDATDASAS"
     gene            1735..2556
                     /locus_tag="PLP95_08785"
     CDS             1735..2556
                     /locus_tag="PLP95_08785"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="coproporphyrinogen-III oxidase family protein"
                     /protein_id="HPB45933.1"
                     /translation="MLSAVPVRHAYVHVPFCASICPFCSFHVKVREAGDVAAYLDRLE
                     EELAEIVAVVPVDLDTLYVGGGTPSYLRTAELARLCEMVERHLGRPRREATLEVHPST
                     ATASRVRHWVELGFTRLSIGAQSFDDEVLRRLGRTHSGADALACIEWALAAAQVTSVD
                     LMMPTAGQDVSADVRRAVATGAQHISVYALTIEPGTPFAMDGVRVDDGGAAEALSAAA
                     DLLGAAGFERYEVSNYARPQARCLHNQAYWAVAPSLGIGPSASGVVCCDDGSLIR"
     gene            2602..2889
                     /locus_tag="PLP95_08790"
     CDS             2602..2889
                     /locus_tag="PLP95_08790"
                     /inference="COORDINATES: protein motif:HMM:NF018652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB45934.1"
                     /translation="MVIESDRDARSDGGGSVEHVHGEDLMRDLLMCGLRRVDGVDLDE
                     VTRRSGVDPRDVRRSELARLSDDALVDIDGALLAATDRGRLLLDQVTVALW"
BASE COUNT          465 a          944 c         1055 g          541 t
ORIGIN      
        1 cgtcgaccac cccgcgcaga cagggcagta ggttcctcgg ccatggcagt caagtccccc
       61 aagccgtaca ccgaggccca ggttcgccgt ggcgacgcgg tcatcaagcg gatgtcgaag
      121 ctcaccacgg cgctgtaccg gcgcacgaat ggacgtatcg gtggccggtt cctacggggc
      181 gccccggtgt gtctgctcac cacgatcggc cgggtctccg gcgaggcgag aaccactccg
      241 ttgctctacc tggccgacgg cgacgacatc gtcctggtcg cttccaaggg tgggtttccc
      301 ggcaacccga actggtatct gaacctgaag gcagatcccg aggtgaccat tcagatcggg
      361 tcggttgccg gcgactacac cgcagcgcag gtatcgccgc aggacaaggc cgaactgtgg
      421 ccccggctgg tggcgatgta ccaggatttt gagacgtatc aggcccgtac cgaccgcgac
      481 atcccagtga tccgctgtcg tcgccgcccc tgattcggtc gaggccgcgg accatggtcg
      541 acggcgctgt gttgcgcgga tccgcacgct tcgggttggc gtgtgggagc attcgtccat
      601 ggaccatcgc accaccgcac gtctgatcgc cgccggacgc accctgttcg gtcttctgtg
      661 tctgctgcgc ccgcgagtac tcaccggatt cgatcgcagt gagccctcgg cggcggcgct
      721 cgggtggatt cgactgttcg gtgtgcgcga tgtcgtgttg ggcgccggca cgctggcgag
      781 cctcaacggc gacgatggcg acacgaggtg ggtgaagatg tcggcggccg ccgacaccgc
      841 cgacgcggtc accgtggtca cggtccgcga cgaggtcggc gtttcgttca tggcggcttc
      901 gttggcgctc gcggtgccgg ccgcagcgct ggggtggcgc gccgcgttcg ggctgggtcg
      961 cagccgcggc tgaggttctg cgaatcgaat cggccgaacg ggtagggttc ggacgtgtcg
     1021 ttcaaagcga agttttcgaa gctgatcctc aagctgtggc gctggacgcc cgtgggggag
     1081 cgcccggagt tcagttgtgt gatcaccgcg gggccacaca ccgccaacat cgattacgtc
     1141 ctcatgatgc tcatggcgtg gtcccggggc atggaactgt cgtggatggg caagcacacc
     1201 ctgttcgaaa agccgatcct cggtaagttc ttcaccgcca ccggcggaat cccggtcgat
     1261 cgcacggcgc cccagggcct ggtcggccaa atggtcgaac gcttcgaaca gaccccacgg
     1321 ctgtatctcg cgatccctcc ggaggggacc cgcagtctca ccgaatactg gaagtcgggc
     1381 ttctaccgca tcgctcaggg cgcgggggtg ccgatcgtga tgtgttcgat cgatacgaag
     1441 acccgtgtgg tcgagatcgg accgacggtg catccgagcg gcgacctcgt cgccgacatg
     1501 gatctcttcc gcgagtacta cggccccaag gtcggcttca aaccggagct gtacggcccg
     1561 atccgcctcc gcgaggagga ggccctcggc gacggcgacg ccctcggcga tggcaacgcc
     1621 gacggcgcga accccggcga cgccacggac gcgtcggcca gctgattcgc agatcgatcc
     1681 cgcaggtcga tccgtgggct gaagccggtg cagtgtggcc ctacggtggg tggggtgctg
     1741 tctgcggtac ccgttcgtca tgcgtacgtg cacgttccgt tttgtgcgtc gatctgcccg
     1801 ttttgcagct tccatgtgaa ggtgcgcgag gccggtgatg tggcggccta cctcgatcgg
     1861 ctcgaggagg aactcgccga gatcgtggcg gtggtgccgg tcgatctcga caccctgtat
     1921 gtgggtggcg gaacgccgtc gtatcttcga acggcggaac tggcccgact gtgtgagatg
     1981 gtcgaacggc acctgggtcg cccgcgccgc gaggccaccc tcgaggtgca cccctcgacc
     2041 gcaaccgcgt cgcgggtgcg ccactgggtc gaactcggat tcacccggct cagcatcggc
     2101 gcccagagtt tcgacgatga ggtgctgcgg cgcctcggcc gcacccatag cggtgccgac
     2161 gcgttggcct gtatcgagtg ggccctcgcc gcggcgcagg tgacctcggt agacctgatg
     2221 atgccgaccg cgggtcaaga cgtgtcggcg gatgtgcgcc gggcggtcgc caccggggca
     2281 caacacatct cggtgtacgc gttgacgatc gagccgggaa cacccttcgc gatggacggg
     2341 gttcgcgtcg atgacggggg tgccgctgag gcgttgagcg ccgctgccga tctgctgggt
     2401 gccgccgggt tcgaacgcta cgaggtgtcg aactatgcgc gaccgcaagc gcgttgcctc
     2461 cacaatcagg cgtactgggc ggtggcgccg tcgctcggca tcggcccctc ggcgtcgggg
     2521 gtcgtgtgct gtgacgacgg ttcgttgatc cgctgaacca atccgacact cgatcggtgg
     2581 cgcgtcgcca ggtcgccgct catggtcatc gagtcggatc gcgacgcccg aagcgacggc
     2641 ggcggctccg tcgaacatgt gcatggcgaa gacctgatgc gcgacctgct gatgtgcggg
     2701 cttcgacggg tggacggggt cgatctcgac gaggtgactc gccgaagcgg cgtcgaccct
     2761 cgcgatgtgc gtcgcagcga actggcacgc ctgagcgacg atgcgctggt cgacatcgac
     2821 ggggcgctgt tggcggcgac cgaccgtgga cggttgctgc tcgaccaggt caccgttgcg
     2881 ctctggtgac cgggtgcccg aggagcgatc ccgagagatc agatcttggc tcgggccgag
     2941 tatctcggta cattccattt cagcatttgt ggtcagctgg gagaaacaag atggcatgga
     3001 gtggg
//
LOCUS       DAOOMA010000314         3002 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_39930_length_3002_cov_3.687818, whole genome shotgun sequence.
ACCESSION   DAOOMA010000314 DAOOMA010000000
VERSION     DAOOMA010000314.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 3002)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3002)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3002
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39930_length_3002_cov_3.687818"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1284)
                     /locus_tag="PLP95_08795"
     CDS             complement(<1..1284)
                     /locus_tag="PLP95_08795"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB45935.1"
                     /translation="MRSFLQFLIIGLGSGATYALFAQGAVVIYRGSGLVNFAQGAVGT
                     LAAYVAFVDLQAESAWPVLPAILVAIAVAGLVSFAFHAIVLRALRDAAPIVRVISTIG
                     MLGLLQAIVAKRYGSGNRPVEAFLPHDVFRVGDLVIQEERVYIVAITIVVTFGLWAWT
                     RYTRTGLAISASAENERLVQTLGWSPARLGAITWTIGGMVGGLAAVLAAPLTGLSAIT
                     FTIVVTLAGLGAALLGGFHSFPLTLVGGLVIGVGESMATLYGADIKDLLHQETITGLN
                     RLAAFIVIIGVVVVRGRGLPLRSHVAAKLPRLGTGEINPIGVLLAGGVTLVVLLGVMN
                     EAWARATYTSLAAGVMLLSIVVITGYAGQISLAQWAFAGIGALIAGRMVRAGVPVELA
                     ILAGILLTIPIGLVFAIPALRTRGVNLAVVTLALGF"
     gene            1556..1924
                     /locus_tag="PLP95_08800"
     CDS             1556..1924
                     /locus_tag="PLP95_08800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB45936.1"
                     /translation="MTRRLRSGIALGLAASLILSACGGDDNADNDTGGTTGGGSSTDT
                     PADLDYAALGLWDDGACDESKPPLIVGTQTVFESPVISLGDVAVALEAAGEAFNKRGG
                     VNGSCIQVITCDDKATSTPL"
     gene            1921..2916
                     /locus_tag="PLP95_08805"
     CDS             1921..2916
                     /locus_tag="PLP95_08805"
                     /inference="COORDINATES: protein motif:HMM:NF024850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HPB45937.1"
                     /translation="MNCARDLDDKGVQVVVNDLVTAGASEVAGVLSGAGIPRVATNLT
                     PGNYDDDMAFPLNASSSGAVFLLPKGLVESGINKIGILRPDLSESAIMQSLFEQVYGA
                     DGAEFVSDAPVASGTTDYTQYILSASDAGAEGVVLATGEQEAVQLVKAGQQLATDLKF
                     GISLGTFSHKNVTDLGDTAEQMVFVGAFPPATMDVPVYEVLRADLAASGDDRLQPDTL
                     KTSAQQSWIGLYATIKMLRDANVTDFSRENVTAAIRAAKDVPMLGIFGDETWTPDVQI
                     EGLFARPGIPTWGIYSWNPDAEAPDGLSGNFEETTTVDFVDILCGTPLGAPAETC"
BASE COUNT          570 a         1025 c          940 g          467 t
ORIGIN      
        1 gaacccgagc gccaacgtga cgaccgccag gttgacgccg cgggttcgca atgcggggat
       61 ggcgaacacc aacccgatcg ggatggtcaa caagatgccg gcgaggatgg ccaactcgac
      121 cggcactccg gcccgcacca ttcgcccggc gatcagcgcc ccgattccgg cgaaggccca
      181 ctgagccagc gagatctgtc ccgcataccc ggtgatcacc acgatcgaca gcagcatcac
      241 cccggcggca agggaggtgt aggtggcacg cgcccacgcc tcgttcatga cgccgagcag
      301 caccaccaac gtgaccccgc ccgccagcag gaccccgatc gggttgatct caccggttcc
      361 gagacgaggc agcttcgcag cgacgtgtga acgcaacggc aaccctcggc ctcgcaccac
      421 gacgaccccg atgatgacga tgaacgctgc gagccggttc agcccggtga tcgtttcctg
      481 gtgcaacagg tccttgatgt cggcgccgta gagcgtggcc atcgactcac cgacgccgat
      541 gaccagcccg ccgaccagcg tgagcgggaa cgagtggaac ccgccgagca gcgcggcgcc
      601 gaggccggcg agggtgacca cgatggtgaa ggtgatggcc gacagcccgg tgagcggcgc
      661 ggccagcact gctgcgagcc caccgaccat gccgccgatc gtccaggtga tcgcaccgag
      721 gcgcgccggg gaccagccga gggtctgcac gagtcgttcg ttctcggcgc ttgcgctgat
      781 cgccaggccg gttcgggtgt agcgggtcca tgcccacagt ccgaaggtca cgacgatggt
      841 gatggcgacg atgtagacgc gctcttcctg gatgacgagg tcgcccaccc ggaacacgtc
      901 gtggggaagg aacgcctcga cgggccggtt tccgcttccg tatcgcttgg cgacgatcgc
      961 ttgaagcagt ccgagcatgc cgatcgtgga gatcacccgc acgatgggcg ccgcatcacg
     1021 tagcgcccgc aggacgatgg cgtgaaatgc gaacgagacc aggccggcga cggcgatggc
     1081 gacgaggatc gccggtagca ccggccaggc gctttcggct tgaaggtcga cgaacgcgac
     1141 gtacgcggcg agggtgccca ccgccccctg cgcgaagttc accagacccg aacctcggta
     1201 gatcaccacc gcgccctgag cgaagagcgc gtaggtcgcc cccgagccca ggccgatgat
     1261 cagaaactgt aggaacgaac gcacattttc ccctgtttgc gtcccgatcg acgatcgaac
     1321 gtcacgctgt tgcccggccg ccgcgcgcac cccacgcggt aaacgctcac ttagttgtgc
     1381 tgcagcgtag tgggcgcacc tgccgcaacg tcggcatgat tgtagtcttc gcgagaaaag
     1441 agaacctgga ttacgttttc gccaacaccg cctcgcgaaa tgcggtagac ccagtgcgac
     1501 acttgtcaca caatcggtcg agaccccggc cgatcgcaac gaaggaaggg ttcgcatgac
     1561 acgacgactc cgaagcggaa tcgcgctcgg cctggccgca tcgctgatac tgagcgcctg
     1621 tggcggcgat gacaacgcag acaacgacac cgggggaacc accggcggag gcagctccac
     1681 cgacacgccc gccgacctcg actacgccgc gctcggcctc tgggacgacg gtgcgtgcga
     1741 cgaatccaag cccccactca tcgtcgggac ccagacggtg ttcgaatcgc cggtcatctc
     1801 cctcggcgac gtcgccgttg cgctcgaagc cgctggtgag gcgttcaaca agcgcggcgg
     1861 tgtcaacggc tcgtgcattc aggtcatcac ctgcgacgac aaggcaacct cgacgccgct
     1921 gtgaactgtg cacgtgacct cgacgacaag ggcgttcagg tcgtggtcaa cgacctggtg
     1981 accgccgggg cctccgaggt cgccggtgtc ttgtccggcg ccggcattcc acgcgtcgcc
     2041 accaacctca cccccggcaa ctacgacgac gacatggcct tcccgctgaa cgcgtcgagt
     2101 tccggtgcgg tgttcctgct gccgaagggt ctggtcgagt cgggcatcaa caagatcggc
     2161 atcctgcgcc cggatctgtc ggaatcggcg atcatgcagt cgctgttcga gcaggtctac
     2221 ggcgcagatg gtgcggagtt cgtttcggat gctccggtcg cctccggcac caccgactac
     2281 acccagtaca tcctgtcggc ctccgatgcc ggcgccgaag gcgtggtgct cgcaaccggc
     2341 gagcaggaag cggtgcagtt ggtgaaggcc ggccagcagt tggcgaccga cctcaagttc
     2401 ggcatcagcc tcggcacgtt ctcccacaag aacgtcaccg acctcggcga caccgccgaa
     2461 cagatggtct tcgtcggggc cttcccaccc gccacgatgg acgtgccagt ctacgaggtt
     2521 cttcgcgccg atcttgcggc ctctggcgac gaccgcctgc aacccgacac cctcaagacg
     2581 tctgcccagc agtcatggat cgggttgtat gcgaccatca agatgcttcg cgacgccaac
     2641 gtcaccgact tctcccggga gaacgtgacc gccgcgatcc gtgcagcgaa ggacgttccg
     2701 atgctcggca ttttcggtga cgagacctgg accccggatg tgcagatcga gggcctcttc
     2761 gcccgacccg gcattccgac ctggggcatc tacagctgga accccgacgc cgaagcgccc
     2821 gacggactct caggcaactt cgaagagacc accacggtcg acttcgtcga catcttgtgt
     2881 ggcaccccgc tgggggctcc tgcagaaacc tgctgagcca gcctcccgac tcgttctgag
     2941 tcagccaccc ggctcgttct ccgataacca gtggtcgtga tcacgaccac tggttatcgg
     3001 ag
//
LOCUS       DAOOMA010000315         2995 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_40062_length_2995_cov_4.260204, whole genome shotgun sequence.
ACCESSION   DAOOMA010000315 DAOOMA010000000
VERSION     DAOOMA010000315.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 2995)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2995)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2995
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40062_length_2995_cov_4.260204"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..962
                     /locus_tag="PLP95_08810"
     CDS             <1..962
                     /locus_tag="PLP95_08810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB45938.1"
                     /translation="RFVDLGDLVVATGPVVFTRTGEMTIAVDRWSMVAKCVTPPDPSA
                     PGDPGRRRPDGGPVGGLVEGLVASAAVTRRLCASLESQEFVRYEVSDAEDWEWTLRSL
                     SALGVPRFYTVLHGDDTQGGHPEGPHAEGRRGERARSRDADARGTNAPGADSQGADSQ
                     GADALEVVAAAPYVGTEAIRTIVEEVIVHVADVDLREATWLADGGDIDSGAVGGDIGG
                     PIRTAVDRFDLVVDGQRLATVEAIITDPVEQSGRAESDASVDRLVEVLERGVPPTSTL
                     RISVGALMRAERDDGGSRDERGERELLDERDEGGARSGGQDPL"
     gene            complement(939..1859)
                     /locus_tag="PLP95_08815"
     CDS             complement(939..1859)
                     /locus_tag="PLP95_08815"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HPB45939.1"
                     /translation="MTTVHTALATSRRDVWFWSGPDRCHAWLYVPDREMPASGFATVV
                     MAHGFAATKVMRLDAYAEAFCAAGYAVLVFDYRYFGDSEGEPRELLDIDAQLADWRAA
                     VSFARSQFEVDPDRVALWGTSFAGGHVVATAAQDRRIAAVIAQCPFTDGVASARSSDR
                     SGALTLMRKAIADARSAKRGGEPVRVAVAGRPGDLAMMTAADVADGYASIVPEGTTHR
                     NMIPARIALKVTNYRPGRLARDTRCPLLMYVCMDDSVAPPGAAIKYAGQAPLGEVYRF
                     GGGHFDIYTGDAFVETITAQLEFLQRVLPA"
     gene            complement(1866..>2995)
                     /locus_tag="PLP95_08820"
     CDS             complement(1866..>2995)
                     /locus_tag="PLP95_08820"
                     /EC_number="1.13.12.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015439706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="nitronate monooxygenase family protein"
                     /protein_id="HPB45940.1"
                     /translation="TKATELVGIDVPIFAFSHCRDVVAAVTNAGGCGVLGAVGHSPKQ
                     LDIDLTWIENEVGTKPFGVDLLVPEKFAGADEGGLDRGRLQDLIPEAHRSWLDDLLDR
                     YGVPQLPEGTSSQASVSIGGMRVDPKSMAPLLDVLFSHKTKLVASALGPPPAHFIERA
                     HAADIPVAALAGSVHHAERHVAAGADIIVAQGGEAGGHTGEIATMVLVPEVVDAVGDV
                     AVLAAGGIGRGRQLAAGMALGADGAWCGSVWLTTEEAETTDTIKEKYLQAGLGDTVRS
                     RSITGKPARMLRSPWTEAWEDDSGPGPLPMPLQPMLVGSALARIGRSAKPGTGAYDLT
                     TYFVGQIVGSMNQVKPTKRVVLEMIEEFVETLERLNRLAES"
BASE COUNT          511 a         1003 c         1012 g          469 t
ORIGIN      
        1 cccgtttcgt cgacctcggc gatctcgtcg tggccaccgg tccggtcgtc ttcacacgca
       61 ccggcgagat gacgatcgcg gtcgaccggt ggtcgatggt cgccaagtgc gtcaccccac
      121 ccgacccatc cgctcccggc gacccaggac gccggcgacc cgacggcggc cctgtaggcg
      181 gccttgtcga agggcttgtc gccagcgccg cggtcacgcg tcgactgtgc gcctcgctcg
      241 aatctcagga gttcgttcgc tacgaggtga gcgatgccga ggactgggaa tggacactgc
      301 gatcgttgag cgccctcggg gtgccgcgtt tctacacggt cctccacggc gatgacacac
      361 aaggcggtca tccagaagga cctcatgcag aaggacgtcg tggggagcgt gcccgctctc
      421 gcgatgccga cgctcgcggt accaatgcac ccggagccga ttcccaggga gccgattccc
      481 aaggagccga tgcgctggag gtggtcgcag ccgcgcccta cgtggggacc gaggccatcc
      541 gcaccatcgt cgaagaggtc atcgtgcatg tcgccgacgt cgatctccgc gaagcgacat
      601 ggctggccga cggaggtgac atcgacagcg gcgctgtcgg aggtgacatc ggcggtccta
      661 tccgaacagc ggtcgatcgc ttcgacctgg ttgtcgacgg tcagcggctc gcgacggtcg
      721 aggccatcat caccgatccg gtcgaacagt ccggccgcgc cgagtcggat gcgtcggtcg
      781 accggctcgt cgaggtgctc gaacgcggtg tcccgccaac ctccacgttg cgtataagcg
      841 tcggcgcgct gatgcgcgcg gaacgagacg acggcggatc gcgagacgag cgaggcgaac
      901 gtgaattgct agacgaacga gacgagggcg gagcgcgatc aggcgggcag gacccgctgt
      961 aggaactcga gctgcgcggt gatggtttcg acgaaggcgt caccggtata gatgtcgaaa
     1021 tggccgccgc cgaatcgata cacctcaccg agtggtgcct ggccggcgta cttgatggcg
     1081 gcgcccggcg gtgccaccga gtcgtccatg cacacgtaca tgagcaacgg acagcgcgtg
     1141 tcacgagcga gtcgtcccgg gcgatagttc gtcaccttca acgcgatgcg cgccgggatc
     1201 atgttgcgat gcgtcgtccc ttcgggaacg atcgacgcgt acccatcggc gacatcggcg
     1261 gccgtcatca tcgcgaggtc accggggcgt ccggcaaccg cgacacgcac cggttcccca
     1321 ccacgtttgg ccgagcgggc atcggcgatc gccttgcgca tcagtgtcag cgcaccactg
     1381 cgatccgagg acctcgccga ggcgacaccg tcggtgaacg ggcattgggc gatcaccgcg
     1441 gcgatgcggc ggtcctgcgc cgcggtcgcc acgacatggc ctccggcaaa cgacgtgccc
     1501 cacaacgcca cacggtcggg gtcgacctcg aattgcgagc gggcgaacga caccgcagcg
     1561 cgccaatcgg cgagttgggc gtcgatgtcc aacagttctc gtggctcgcc ctcgctgtcg
     1621 ccgaagtagc ggtagtcgaa gaccaacacg gcatagcccg cggcacagaa cgcctcggca
     1681 taggcgtcga ggcgcatcac cttggtcgct gcgaagccgt gtgccatgac gaccgtcgcg
     1741 aaacccgagg ccggcatctc gcggtcgggg acatagagcc acgcatggca acgatccggc
     1801 cccgaccaga accacacgtc gcgtcgagag gtggccagcg cggtgtgaac ggttgtcatc
     1861 tggcctcagc tttcggcgag tcggttgagt cgttcgagcg tctcgacgaa ctcctcgatc
     1921 atctccaaca cgacccgctt ggtgggcttc acctggttca tcgacccgac gatctgcccg
     1981 acgaagtagg tcgtgaggtc ataggcgccc gtgcctggct tggccgagcg cccgatgcgg
     2041 gcgagcgccg agccgacgag catgggttgc agcggcatcg gcagcggtcc gggacccgag
     2101 tcgtcctccc aggcctcggt ccacgggctg cgcagcatcc gcgccggctt gccggtgatc
     2161 gaccgagacc ggaccgtatc gccaagaccc gcctggagat acttctcctt gatggtgtcg
     2221 gtggtctcgg cctcctcggt ggtgagccag accgacccgc accatgcacc gtcggccccg
     2281 agtgccattc cggctgcgag ttgacggcct cggccgatgc caccggcggc aagcaccgcc
     2341 acgtcaccca ccgcatcgac gacctcgggg acgagcacca tcgtggcgat ttcgcccgta
     2401 tgaccgcccg cctcaccacc ttgagcgacg atgatgtcgg cgcctgcggc gacatggcgt
     2461 tcggcgtggt gcaccgaacc ggcgagcgcg gcgaccggga tgtcggcggc atgcgcacgt
     2521 tcgatgaagt gggccggggg cgggccgagc gccgaggcca cgagcttggt cttgtgcgag
     2581 aacaacacgt cgagcagtgg ggccatcgac ttcggatcga cccgcatgcc gccgatcgac
     2641 accgacgcct gcgaactcgt cccctccggc agttgcggga ccccgtagcg gtcgagaagg
     2701 tcgtcgagcc aggaccgatg ggcctcgggg atgaggtcct gaagcctgcc gcggtcgagg
     2761 ccgccctcgt cggccccggc gaacttctcg ggaaccagca ggtcgacgcc gaacggcttg
     2821 gtgccgacct cgttctcgat ccaggtcagg tcgatgtcga gctgcttcgg cgagtgtccg
     2881 acggcgccga gcaccccgca accaccggca ttggtgaccg ccgctacgac gtcgcggcaa
     2941 tgactgaatg caaagatcgg gacatcgatc ccgacgagtt cggtggcctt ggtgc
//
LOCUS       DAOOMA010000316         2994 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_40082_length_2994_cov_3.725077, whole genome shotgun sequence.
ACCESSION   DAOOMA010000316 DAOOMA010000000
VERSION     DAOOMA010000316.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 2994)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2994)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2994
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40082_length_2994_cov_3.725077"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(111..1229)
                     /gene="dusB"
                     /locus_tag="PLP95_08825"
     CDS             complement(111..1229)
                     /gene="dusB"
                     /locus_tag="PLP95_08825"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HPB45941.1"
                     /translation="MNIGPLVFDTPVVLAPMAGVTNAPFRRLCRRHGAGLYVSEMITA
                     RAYVEGNAKTMRLAHFDEDEPIRSIQLYGIDAHHVGEAVRKLIDSSHVDHIDLNFGCP
                     APKVTRNGGGSALPVRHRLLRDIVRAAVQATGSSCVPVTAKFRMGIDDGHLNFLRTGR
                     ICEDEGCSAVALHARTTEQLYSGDARWDAIGELKASITSIPVLGNGDIWEAADAVAMM
                     RRTGCDGVVIGRGCLGRPWLFRDLAAALGGSPIPEAPTFGEVVDMMLEHLDLLIDWAG
                     QWIGVREFRKHTGWYLKGYATGGDVRRELNQLESREQLADTLGRFDRSSTMSSEGRRA
                     KRGHVGGPRAVSLPDRWFDNEDAIDALAADAESISSGG"
     gene            1417..1923
                     /locus_tag="PLP95_08830"
     CDS             1417..1923
                     /locus_tag="PLP95_08830"
                     /inference="COORDINATES: protein motif:HMM:NF014647.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HPB45942.1"
                     /translation="MKRNAWIAALLAGSLLASACGKSDDSGSDDTDSGSSTGQAGSDD
                     VVKVAWILPGPINDGGWSTAHNEGRLAVEEHFGDKVETTYKESVADGPQADQVVADLV
                     KDGYDLIFGASFGFGESLIQASEANPDIAFEHATGIEPLDNMGIYFGAGEQSLSPAWP
                     RAPRPRPT"
     gene            1890..2546
                     /locus_tag="PLP95_08835"
     CDS             1890..2546
                     /locus_tag="PLP95_08835"
                     /inference="COORDINATES: protein motif:HMM:NF014647.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HPB45943.1"
                     /translation="MAAGAETTSNLIGFVAPFPIPEVIRHINAYTLGAQAMNPDAQVK
                     VVWTNSWFDPETERKAAESLIADGADVIANGGDGPAPGEAAQAAGVAWTGYDADQSEN
                     FPDIWLTAALYHWGDYYIGQVQKLIDGTWEPGDYYGYIADGLTDIAPFGKRVSAETQA
                     KIMEKRDEYIAGTFDETAGPIYDQDGELKIPEGEVMSHADQMSVMWFVKGVIGTIPSS
                     "
     gene            2550..>2994
                     /locus_tag="PLP95_08840"
     CDS             2550..>2994
                     /locus_tag="PLP95_08840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015439714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HPB45944.1"
                     /translation="MADRPTAGAVGAESSTAPAVSMQKITKRFPGGVVANHDVDFDLA
                     VGEIHALLGENGAGKSTLSNVLTGIYQPDAGTMSLYGEPTVISSPRDAIERGIGMVHQ
                     HFRLVETMTVAENIALGLHGPLRRRKLEDRVRELGQRYGLVVDPTA"
BASE COUNT          545 a          971 c          999 g          479 t
ORIGIN      
        1 gaagccgaac cgcctgcagg tcgtgctcgg gctcagccgg cgcggcgagg ccgaagcgag
       61 gatcaaccga cgcccggcac cacggccggc ggtgggtgca gggtccatcg tcaccctccc
      121 gaactgatcg actcggcgtc ggccgccagg gcgtcgatcg cgtcctcgtt gtcgaaccag
      181 cgatccggca gcgacaccgc ccgcggaccg cccacatggc cgcgcttcgc gcggcggccc
      241 tcggaactca tcgtcgacga acggtcgaag cgcccgaggg tgtcggcgag ttgttcgcgg
      301 ctttcgagtt gattgagttc gcgccgtaca tcgccaccgg tcgcgtaccc cttcaggtac
      361 cagccggtgt gcttgcggaa ctcccgaacg ccaatccact gcccggccca atcgatcaac
      421 aggtcgagat gctcgagcat catgtcgacg acctcgccga aggtgggcgc ctccggtatc
      481 ggcgaaccgc cgagtgccgc cgcgagatcg cgaaacaacc acggtcgtcc aagacatcct
      541 cgtccgatca cgacgccgtc gcagccggtg cgccgcatca tcgccacggc gtcggcggcc
      601 tcccagatgt ctccgttgcc caatacggga atcgaggtga tcgaggcctt cagctcgccg
      661 atcgcgtccc agcgggcatc tccggagtac agctgctcgg tcgtgcgggc gtgcagggcg
      721 accgcggagc acccttcgtc ctcacagatc ctgccggtgc gcagaaagtt caggtggccg
      781 tcgtcgatgc ccatccggaa cttggcggtc accggcacgc agctcgaacc cgtcgcttgc
      841 actgcggcgc gcacgatgtc gcgcagcagc cgatggcgca ccggcagtgc ggaaccgccg
      901 ccgttgcgtg tgaccttcgg ggcggggcac ccgaagttca ggtcgatgtg gtcgacatga
      961 gaggagtcga tcagtttgcg aaccgcctcg ccgacgtggt gggcgtcgat cccgtacagc
     1021 tggatgctgc ggatgggttc gtcctcgtcg aaatgagcga ggcgcatcgt cttcgcattg
     1081 ccctcgacat aggcgcgggc ggtgatcatc tcgctgacgt acaacccggc gccgtgtcgg
     1141 cggcacaacc ggcgaaacgg cgcgttggtg acgccggcca tcggggccag caccacggga
     1201 gtatcgaaaa cgagtgggcc gatgttcatg cgcgtaggtg gggttggggc tgaggttcgg
     1261 gcggaagtga aagccgggcc ggggcccggg aggtcgacga cgcccgaaca acggttttac
     1321 acgcgtgtaa cgcgatcctt tgcggcgcgt ttcgttcagg taccagaatc gaccctgttc
     1381 gaccgggatc accggctgac acgaaaggct caaacagtga agcgaaatgc atggatcgcc
     1441 gctcttttgg cgggttcgct gcttgccagc gcatgtggca agtcggacga ttccggctcg
     1501 gacgacacgg attcaggatc ctcgaccggc caggccggct ccgacgacgt ggtgaaggtg
     1561 gcctggattc tccccgggcc gatcaacgac ggcggctggt cgacggcgca caacgaggga
     1621 cgtctcgccg tcgaggagca cttcggtgac aaggtcgaga ccacgtacaa ggaaagcgtc
     1681 gccgacggtc cccaggccga ccaggtggtc gccgacctgg tcaaggacgg atacgacctc
     1741 atcttcggtg cgtccttcgg tttcggcgag tcgctcatcc aggcgtctga agcgaacccc
     1801 gacatcgcgt tcgaacacgc gacggggatc gagccgctcg acaacatggg tatctatttc
     1861 ggagcgggcg aacagtcgct ctcaccggca tggccgcggg cgccgagacc acgtccaacc
     1921 tgatcggttt cgttgcgccc ttcccgatcc cggaggtgat ccgtcacatc aacgcctaca
     1981 ccctcggcgc acaggcgatg aacccggacg cgcaggtgaa ggtcgtgtgg accaactcct
     2041 ggttcgatcc cgagaccgag cgcaaggccg cggagtcgtt gatcgccgat ggtgccgacg
     2101 tgatcgccaa cggcggcgac ggtccggccc cgggcgaggc cgcgcaggcc gctggggtgg
     2161 cctggacggg ttacgacgcg gatcagtcgg agaacttccc cgacatctgg ctgaccgcgg
     2221 cgctgtatca ctggggcgac tattacatcg ggcaggtcca gaaattgatc gacgggacct
     2281 gggaaccggg cgactactac ggctacatcg ccgatggcct gaccgacatc gccccgttcg
     2341 gcaagcgggt gagcgccgag acccaggcca agatcatgga aaagcgcgac gagtacatcg
     2401 ccggaacctt cgacgagacc gccgggccga tctacgacca ggacggcgaa ctcaagattc
     2461 cggagggcga ggtgatgagc cacgccgatc agatgagcgt catgtggttc gtcaagggcg
     2521 tcatcggaac gatcccgtcg agctgacagg tggccgaccg tccaacagcg ggtgccgtcg
     2581 gggctgagtc ctcgacggcg ccggcggtgt cgatgcaaaa aatcaccaag cgattccctg
     2641 gcggcgtagt agcgaaccac gacgtcgact tcgacctcgc cgtcggtgag atccacgcgc
     2701 tgttgggcga aaacggtgct ggcaagtcga ccctgtcgaa tgtcctcacc ggcatctacc
     2761 aaccagacgc cggaacgatg tcgctgtacg gcgagccgac ggtcatctcg tcgccgcgcg
     2821 acgcgatcga gcgcggcatc ggcatggtgc atcaacactt tcgcctcgtc gaaacgatga
     2881 cggtcgccga gaacatcgcg ctcggcctcc acgggccgct tcgacgtcgc aagcttgagg
     2941 accgggtgcg tgagctcgga cagcgctacg ggctcgtcgt cgacccgacc gcca
//
LOCUS       DAOOMA010000317         2991 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_40145_length_2991_cov_2.782016, whole genome shotgun sequence.
ACCESSION   DAOOMA010000317 DAOOMA010000000
VERSION     DAOOMA010000317.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 2991)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2991)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2991
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40145_length_2991_cov_2.782016"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..252
                     /locus_tag="PLP95_08845"
     CDS             <1..252
                     /locus_tag="PLP95_08845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB45945.1"
                     /translation="CVGRIRYLGVLLYDADRIAAAAGVEHDLNLYSAQLQVFLDPNDL
                     KVIAQARLDGIPESWIDAARNSPARAPSLSTSARAVTFC"
     gene            complement(458..1939)
                     /locus_tag="PLP95_08850"
     CDS             complement(458..1939)
                     /locus_tag="PLP95_08850"
                     /inference="COORDINATES: protein motif:HMM:NF013279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HPB45946.1"
                     /translation="MMTSSRGRTDPRRRLPDARATRSRIRRERREVARGEIPTEFWAL
                     LASVTMLCCVGLVAVFSSTTVRSIAGGDAPTAFLSRHALFLGIGAVLMFAGYKVGYRR
                     VSLLAPLALGSAATLLVAVLIFGSTYNGARRWFSFGFVTFQPSELAKLAIIVWVARLL
                     AMREREMHLPGRTVMPVAVVVGLAGFVIYLEPDLGTAVVLAAVAATMLVVAGARLDAL
                     FGLAIPVGATFLALSQRGYHKDRWGFLDPLSNANHENYQLLGGLSSIANGGWFGVGPG
                     GSSAKWGYLPEAESDAIFAVVAEEFGILGAMLVIGLYVVILVCGIRVAYKAADRFGRL
                     LAIGITTMISIQTFVNIGVVLGVLPNKGFTLPFISYGGTSLVMSLAAMGLLLSVAREG
                     DAAQARLRRRQRSNRRLAKRAPSRGALVLAASDGRSTESRPRRRTPAKSAARQPAARK
                     PAARQPAAKKPAARQPARQPAAKKSAPRQPAQRRSSTGRAAQR"
     gene            complement(1936..>2991)
                     /gene="murD"
                     /locus_tag="PLP95_08855"
     CDS             complement(1936..>2991)
                     /gene="murD"
                     /locus_tag="PLP95_08855"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HPB45947.1"
                     /translation="QAFDDRPFVSVTGTNGKTTVVTLVVEMLEASGVRSLAVGNTEIP
                     FVEAIGRDDVSVFVVEASSFRLAQTRRFLPAAAAWLNFAPDHLDVHRSMLHYEAAKAR
                     SWRDLGPAQMAVANAEDPIVSGYIETGEATYVTFGLSPDSARPTIGHYSVVDGRLVTP
                     EGDTLIAVHDMARNLPHDIANALAAAALARTVGATPEGIASALTHFSGLAHRVQTIAT
                     VDGVRYVDDSKATAPHAVRAAVESFETVVLIAGGRNKGLDLSELATLRDRVRAVVGIG
                     ESGPEVVSVLRPRPGRSVSSMREAVEAAKGFAQPGDVVLLSPGCASFDQYENYAERGD
                     DFVAAVAAVTATAGEPR"
BASE COUNT          525 a         1063 c          967 g          436 t
ORIGIN      
        1 tgcgtcggcc gcatccgcta cctcggcgtg ctgctgtacg acgccgaccg catcgccgcg
       61 gccgccggcg tcgaacacga cctgaatctc tattcggcgc aactgcaggt tttccttgac
      121 ccgaacgacc tcaaggtcat cgctcaggcg cgtctcgacg gcattcccga gtcctggatc
      181 gacgcggcgc gcaacagccc ggcaagggca ccgagtttgt cgaccagtgc ccgcgcagtg
      241 accttttgtt gaatgccccg gcctggcaac gccacgagtt cgatgccgct cgggggtacc
      301 agcgcaacct cctggccacg gtcggacccc acccacacca ggtcgccccg cgcgacgccc
      361 ttcgcgacca gcgcctcggc gatcgagagt ccgggcagga tgtgtccggc ggtgcctcca
      421 cccgcgatga cgatcggtcg agacgtctcc gtcccggtca tcgctgtgcc gcccgaccgg
      481 tcgacgaccg acgctgtgca ggctgccgcg gtgcagattt cttcgctgca ggttgccgcg
      541 cgggctggcg cgcagcaggc ttcttcgctg ccggctggcg cgcagcaggc ttccttgctg
      601 cgggctggcg cgctgcggac ttcgcggggg tgcgccgccg cgggcgcgat tcggtcgacc
      661 tgccgtcgct cgccgccagc accagggcac ctcgagacgg tgcccgcttg gccagacgcc
      721 ggttcgaacg ttggcgacgg cgaagccgcg cctgggcggc gtcgccctcg cgggcaacgc
      781 tcagaagcag gcccatcgcc gcaaggctca tcaccagcga cgtgcctccg tagctgatga
      841 acggcagcgt gaatcccttg ttcggcaaca cgccgagcac cacgccgatg ttcacaaacg
      901 tctggatcga gatcatcgtc gtgatgccga tggccaagag ccggccgaac cgatccgccg
      961 ccttgtaggc gacccggatg ccgcagacca ggatcacgac atagagcccg atcacgagca
     1021 tcgcgccgag gattccgaac tcctcggcga ccaccgcgaa gatggcgtcg ctttccgcct
     1081 ctgggagata cccccacttg gcggacgacc caccggggcc gacgccgaac cagccgccgt
     1141 tggcgatact cgaaagcccg ccgagcaact ggtagttctc gtggtttgcg ttcgacaggg
     1201 ggtcgaggaa gccccaacgg tccttgtgat atccgcgctg ggacaacgca agaaacgtcg
     1261 caccaaccgg aatggccagc ccgaacagcg cgtcgagtcg cgccccggcg acgaccaaca
     1321 tcgtggcggc caccgccgcc aacaccaccg cggtgccgag gtcgggttcg aggtagatga
     1381 caaacccggc caacccgacc acgacggcga ccggcatcac cgtgcgtcct ggcagatgca
     1441 tctcgcgttc gcgcatcgcc agcagccggg cgacccacac gatgatggcg agtttggcca
     1501 actccgacgg ctggaaggtg acaaagccga aggagaacca acgccgggcg ccgttgtagg
     1561 tgcttccgaa aatcagcacc gcgaccagca gggttgcggc ggaccccagg gcgagcggag
     1621 ccaacagcga cactcgccgg tacccgacct tgtagcccgc gaacatcagg accgcaccga
     1681 tgccgagaaa caacgcgtga cgcgacaaga aggccgtcgg tgcatcaccg ccggcgatgg
     1741 agcgcaccgt ggtcgacgaa aacaccgcca ccaggccgac gcaacacagc atggtcaccg
     1801 aggccagcaa cgcccagaac tcggtcggta tctcccctcg ggcaacctca cgccgctcgc
     1861 gtcgaatccg cgaccgggtt gcccgggcgt ccggaagtcg gcgacgtggg tcggtgcgcc
     1921 ctcgcgatga cgtcatcatc gcggctcacc cgccgtggcg gtgaccgctg cgaccgccgc
     1981 gacgaaatcg tcgccgcgct cggcgtagtt ctcgtactga tcgaacgagg cgcaacccgg
     2041 gctcaacaac acgacgtcgc cgggctgagc aaaccctttc gccgcctcca ccgcttcgcg
     2101 catcgacgag acgctacggc ctgggcgcgg ccgaaggacg gataccacct cggggccgct
     2161 ttcaccgatt ccgacgacgg cgcggacccg atcccgcagg gtcgcaagct ccgacaggtc
     2221 gaggcctttg ttgcggccgc cggcgatcaa cacgaccgtt tcgaagcttt ccaccgcggc
     2281 gcgcaccgca tgcggggcgg tggccttgga gtcgtcgaca tagcgcacac cgtcgacggt
     2341 cgcgatcgtt tgaacccgat gggccaaccc cgaaaagtgc gtgagcgcgc tggcgattcc
     2401 ctccggggtc gctccgaccg ttctggccaa cgctgcggcc gccaacgcgt tcgcgatgtc
     2461 gtgaggcaga ttgcgagcca tgtcgtggac cgcgatgagc gtgtccccct cgggggtgac
     2521 gagccgacca tcgaccacgc tgtagtgccc gatggtcggg cgtgccgagt ccggcgacag
     2581 gccgaaggtc acatacgtgg cctcccccgt ctcgatgtac ccgctcacga tcgggtcctc
     2641 ggcgttggcc accgccatct gagcggggcc gaggtcgcgc cagctgcgcg ccttggccgc
     2701 ctcgtagtgc agcatcgatc ggtggacgtc gaggtggtcc ggagcgaagt tcaaccacgc
     2761 ggctgccgcc gggaggaaac gccgggtctg agcgagtcga aacgacgagg cttccaccac
     2821 gaacaccgac acgtcatcgc gaccgatggc ctccacgaag gggatctcgg tgttgcccac
     2881 cgcgaggctg cgaacccccg aggcctcgag catctccacc accagggtga cgacggtggt
     2941 cttgccgttg gttccggtca ccgacacgaa cgggcgatcg tcgaaggcct g
//
LOCUS       DAOOMA010000318         2990 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_40171_length_2990_cov_3.141397, whole genome shotgun sequence.
ACCESSION   DAOOMA010000318 DAOOMA010000000
VERSION     DAOOMA010000318.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 2990)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2990)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2990
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40171_length_2990_cov_3.141397"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(51..638)
                     /locus_tag="PLP95_08860"
     CDS             complement(51..638)
                     /locus_tag="PLP95_08860"
                     /inference="COORDINATES: protein motif:HMM:NF014664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HPB45948.1"
                     /translation="MTDDLNPRIAPLPPQRWPREMKDALAPLSAERYGRPPQPPGRPK
                     GLNVLGTFAHHPTLAAAFMQFNAHVMHTTTLTERQRELVVLRVATVYHCDYERAQHEI
                     IAGDFNITADDLDRIAAGPDAPGWSPFDSALLRAVDELVADAQMGEDTYAALRREFDE
                     QQVLDLVFTVGAYGVLAMALNVCGTPLDDDLRPPA"
     gene            complement(635..1435)
                     /locus_tag="PLP95_08865"
     CDS             complement(635..1435)
                     /locus_tag="PLP95_08865"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HPB45949.1"
                     /translation="MTSNSDSDTDSDSTPTRNPARRTVVITGAGGSLGAALATHFAAD
                     PATNLVLSDLSDASLSATLDAIEAAGASVNLDTRLADVSEPAAVQAVVDLALERFGAV
                     DAMISNAGVLSPNGRIHNLTPEDWQRAFNVNVMGAVNAISAVVPSMRERRSGSIILTA
                     SVSGLTAWSHAAPYCATKAAVIQLAKVAAVEYSRDNIRVNCVCPGTFRSAIHEQLPQQ
                     AIDTIAAKHPLGLGTAADLVGAYDYLASDASRWQTGSAVVVDGGYSAP"
     gene            complement(1432..2358)
                     /locus_tag="PLP95_08870"
     CDS             complement(1432..2358)
                     /locus_tag="PLP95_08870"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HPB45950.1"
                     /translation="MSGYRTGRYKILGANRDPNFLGDAVGDTSEAAQVEAAQVEAAQV
                     EAAQTEAAQIEIPATWPNPGDVAGKRVVLTGASRGLGRLLAHGFSSAGAKLTLVARTR
                     HDLVALASELPGPSIIVVGDVSDEEVNERIAADTIAEWGGLDVWISNAGISPVVADAR
                     SVDLDTWRHVLEVNLTSAFLGARAAAKVMQPGGRIVFTGSVLGERSARGLGAYGASKA
                     GLVALAKSLALDFADEGITVNVVAPGWFDSPLAEGWKKNPKLADRVLSHTAQHRWGAN
                     TDLVGAYQFLASDASAFITGTVINVDGGYLLV"
     gene            2357..>2990
                     /locus_tag="PLP95_08875"
     CDS             2357..>2990
                     /locus_tag="PLP95_08875"
                     /inference="COORDINATES: protein motif:HMM:NF012880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HPB45951.1"
                     /translation="MAGNDELLEMYRRMAVIRGFEQRVSVLYRNGEVPGFVHLSIGQE
                     ATAVGACWALRSDDVITSTHRGHAHCLAKGLDPTAMFAELMGRDGGTNRGRGGSMHIA
                     DPNLGIFGANGIVAAGLPIAAGAATAAQLRADDAVVVAFFGDGAVAQGAFHEAVNLAA
                     VWELPVVFFCENNGYAEFSPAEAQHAAPLSSRAAGYGVGFTAIDGNDVAGV"
BASE COUNT          460 a          918 c         1096 g          516 t
ORIGIN      
        1 agaacggctg tggcgggtgg gcgcgggcgc ggcagtgggg cgggcgcggg tcaggcgggt
       61 gggcgcaggt cgtcgtcgag cggggtgccg cacacgttga gcgccatggc gaggacccca
      121 tacgcgccga cagtgaagac gaggtcgagc acctgttgtt cgtcgaactc ccggcgcaac
      181 gcagcgtagg tgtcctcgcc catctgcgcg tcggccacca gttcgtcgac ggcccgcaac
      241 agcgccgagt cgaacggtga ccaaccgggg gcgtctggtc cggcggcgat gcggtcgagg
      301 tcgtcggcgg tgatgttgaa gtcgccggcg atgatctcgt gttgggcccg ctcgtagtcg
      361 cagtgataca ccgtggcgac ccgcaacacg acgagttcac gctggcgctc ggtgagggtc
      421 gtggtgtgca tgacgtgcgc gttgaattgc atgaacgccg cggccagcgt cgggtgatgg
      481 gcgaaggttc ccaacacgtt gagccccttg ggccgaccgg gcggctgggg aggtcgcccg
      541 tagcgttcgg cgctgagcgg agcgagggcg tctttcatct cccgaggcca gcgttgcggc
      601 ggcaggggag cgatgcgagg gttgaggtcg tcggtcatgg tgctgaatat cctccgtcga
      661 cgacgaccgc cgagccggtt tgccatcggg acgcgtcact tgccagatag tcataggccc
      721 cgacgagatc cgcggcggtg ccgagtccga gcgggtgttt cgcggcgatg gtgtcgatgg
      781 cctgttgggg caattgttcg tggatcgctg agcgaaacgt tccggggcac acgcagttca
      841 cccggatgtt gtcgcggctg tattcgaccg ccgcaacctt ggcgagttgg atgaccgccg
      901 ccttggtcgc acagtacggc gcggcgtgag accatgcggt cagccccgac accgatgcgg
      961 tcaggatgat cgacccggat cgtcgctcgc gcatcgaggg cacgaccgcg gagatggcgt
     1021 tgaccgcccc catgacgttg acgttgaagg cccgttgcca gtcttcgggc gtcaggttgt
     1081 ggatccgacc gttgggcgac agcaccccgg cgttggagat catggcgtcg acggcgccga
     1141 agcgttcgag cgcgagatcg accaccgcct gcacggcggc cggttcgctg acatccgcga
     1201 gccgggtgtc gaggttgacg gaggcgcctg cggcctcaat ggcgtcgagg gttgcgctga
     1261 gggaggcgtc gctgaggtcg ctcaacacga ggtttgtcgc cgggtcggcg gcgaagtggg
     1321 tggcgagcgc ggcgccgagc gagccgcccg ctccggtgat gacgacggtg cgtctagccg
     1381 ggttccgggt cggggtcgag tcagagtcag tgtcagagtc agagttgctg gtcatacgag
     1441 gagataccct ccgtcgacgt tgatgacggt gccggtgatg aacgccgagg cgtccgaggc
     1501 gaggaactga taggccccga ccaggtcggt gttggccccc cagcggtgtt gggccgtgtg
     1561 gctgaggacc cggtcggcga gcttgggatt cttcttccat ccctcggcga gcggggagtc
     1621 gaaccatccg ggggcgacga cgttcaccgt gattccctca tcggcgaaat cgagggccag
     1681 gctcttggcg agggcgacga gtcccgcctt ggaggccccg taggcgccga gtccacgtgc
     1741 cgatcgttcg ccgaggaccg aaccggtgaa cacgatccga ccaccgggtt gcatcacctt
     1801 cgcggctgct cgggcgccga gaaaggccga ggtgagattg acctcaagca cgtgtcgcca
     1861 cgtgtcgaga tccactgagc gggcatcggc gacgacgggg gagattccgg cgttggagat
     1921 ccacacgtcg agtccgcccc attcggcgat cgtgtctgcg gcaatgcgtt cgttgacctc
     1981 ttcgtcggag acgtcaccca ccacgatgat cgaggggccg ggtaattcgc tggcgagggc
     2041 gacgagatcg tgacgggttc gtgccaccag cgtgagtttg gctccagcgg aggagaaccc
     2101 atgggcaagg aggcggccaa ggccacggct cgcaccggtc agcacgactc gctttcccgc
     2161 gacgtcgccc gggttgggcc acgtcgccgg aatctcgatc tgtgccgctt cggtctgtgc
     2221 cgcctcgacc tgtgccgcct cgacctgtgc cgcctcgacc tgtgctgcct cggatgtgtc
     2281 gcccacggcg tcccccagaa agttcggatc ccgattcgcc cccaggatct tgtaccgacc
     2341 ggtacgataa ccgctcatgg ccggaaacga tgaacttctc gagatgtatc gacgcatggc
     2401 ggtgatccgc ggcttcgaac agcgcgtctc ggtgctctac cgcaacggtg aggtgccggg
     2461 gttcgtccac ctgtcgatcg gccaggaggc caccgcggtc ggtgcgtgct gggcgctgcg
     2521 gagcgacgac gtcatcacct ccacccatcg cggtcacgcc cactgcctgg ccaaggggct
     2581 cgatccgacg gcgatgttcg ccgaactgat gggtcgcgac ggcggcacca atcgaggtcg
     2641 aggcgggtcg atgcacattg ccgatccgaa cctggggatc ttcggagcga acggcatcgt
     2701 cgccgctggc ctgcccatcg ccgcgggtgc ggccaccgcg gcccagctgc gggccgacga
     2761 tgcggtggtc gtcgcgttct tcggcgacgg tgcggtcgct cagggagcct ttcacgaggc
     2821 ggtgaacctg gcggcggtct gggaactgcc ggtcgtgttc ttctgcgaga acaacgggta
     2881 cgccgaattc tcgccagccg aagcccagca cgcagcgcca ctgtcgagcc gtgccgccgg
     2941 ctatggggtg gggttcacgg cgatcgacgg aaacgacgtc gccggcgtgg
//
LOCUS       DAOOMA010000319         2977 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_40401_length_2977_cov_4.439767, whole genome shotgun sequence.
ACCESSION   DAOOMA010000319 DAOOMA010000000
VERSION     DAOOMA010000319.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 2977)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2977)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2977
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40401_length_2977_cov_4.439767"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..204)
                     /locus_tag="PLP95_08880"
     CDS             complement(<1..204)
                     /locus_tag="PLP95_08880"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HPB45952.1"
                     /translation="MSNATTNAAPTTASLAVESVSVNFGGVMALTEVSFAIPAGQVCG
                     LIGANGASKTTLFDVINGVRVPNA"
     gene            400..816
                     /locus_tag="PLP95_08885"
     CDS             400..816
                     /locus_tag="PLP95_08885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB45953.1"
                     /translation="MSAAPNSDTTSAPGLKNLSGATQIEVEQALDRHGFVGQFGARKG
                     GMVLCFECRSEFSAELVDADAASRIEGASDPSDMAVVVPTTCPSCGTSGTLTMQYGPL
                     SSEEESEVLALISRKAPIPPLDPDGHTAVNAPNPPH"
     gene            complement(891..1286)
                     /locus_tag="PLP95_08890"
     CDS             complement(891..1286)
                     /locus_tag="PLP95_08890"
                     /inference="COORDINATES: protein motif:HMM:NF013970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HPB45954.1"
                     /translation="MIVVDASVLFEVVADTPRSRDLRTLLAAGEALSAPHLIDAEVLA
                     VIQTHLRRGHLDQTAASQAVDDLRSWPGERWSHRLLMPRAWELRNNVRSYDALYVALA
                     EALDAPLVTLDARLAAAPGPLCRIIVAGQ"
     gene            complement(1314..1559)
                     /locus_tag="PLP95_08895"
     CDS             complement(1314..1559)
                     /locus_tag="PLP95_08895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB45955.1"
                     /translation="MSKTVQVRDLDDEVYAALARQAAELQMSVPELLRREITRIAARP
                     SLTEWLKRTERRPCDIQTHDVLAALDELRGPWPGDQT"
     gene            complement(1675..>2977)
                     /locus_tag="PLP95_08900"
     CDS             complement(1675..>2977)
                     /locus_tag="PLP95_08900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB45956.1"
                     /translation="EFVTRRRVDGDEFRPIHRWAWSRAVLVMSSDNDSFELAAVLIAL
                     AVQRSRSAGAQRLHTVYEHSSASKLVRTLKSSLSALRDVDGYGDLVGETGSIAQRLAV
                     VPAALVRRLDAGLDAGLDPAPLLDDALESLSGVNRSGQANITSHELTDLIVELGASAS
                     AGHHDGPMVISDPCVGIGELLLWASRRHGVTADIALCANELDPAVARVTRIRFWLRSL
                     PIELRVGDALKQTGHAGTADVILSDLPTTAGKRDLFRWIRTVNETLRPGGRAVIATTS
                     AILPDWGSTFRGSALELGFGRLEALVLLPAAVKTGSRFDPLIAVLGTNRTASAVVIDL
                     RDAGSQRPKSRRDWFDSGRIAELIDQFRAGTLDEAAGVRIIGVNDLPLVMLPVAFTTS
                     TGEHVPTDRAKQLAANLLNELAEHPQRRAIERALTDGGLLD"
BASE COUNT          524 a          936 c         1000 g          517 t
ORIGIN      
        1 cgcgtttgga acgcgaacac cgttgatgac gtcgaacagc gtggttttgc tggcgccgtt
       61 ggcgccgatc aacccgcaaa cctgacccgc ggggatggcg aagctcacct ccgtgagcgc
      121 catgacgcca ccgaagttca cgctgaccga ctcgacggcg agtgacgccg tggtcggcgc
      181 tgcgttggtc gtggcattgc tcactcgtcc tcccctcaag ttgacgtagg tcccggtcgg
      241 gccgctcgca cacgtgagcg ttcggtccgt acgggtgaca acgtgggctg catgctatcg
      301 agacaggcga cacattcgtc gcgttcgtgt cgatcattcc gtccaggttc tgctttgtcg
      361 gtggctctac gatgtgggct gagcggggat ggaggagcca tgagcgcagc accgaattcc
      421 gacacgacat ctgccccggg gttgaagaac ctcagcggag caacgcagat cgaagttgaa
      481 caggccctcg accgccatgg cttcgtaggg cagttcggtg cgcgcaaggg cggcatggtg
      541 ctgtgcttcg agtgccgcag cgagttctcc gccgaattgg tggatgcaga cgcagcgagt
      601 cggatcgaag gggcctccga cccgtcggat atggccgtgg tagtgccgac gacttgtccg
      661 tcctgtggaa cctcaggaac gctcaccatg caatacggtc cgttgagcag cgaggaggaa
      721 tccgaggtcc tcgcgttgat cagccgtaag gcaccgatcc ccccgctcga tccggatggc
      781 cacacggcgg tgaatgcccc gaacccgccg cactgacccc gccgcactga cggtgtcgca
      841 ctgatgctgt tggaacgcct ggtgaacgct gtcggcgatg gtgagccgac ctactggccg
      901 gcaacgatga tccgacagag cggcccgggc gcagcggcga gtcgggcatc gagcgtgacg
      961 agcggtgcgt cgagggcctc cgcgagtgcc acgtataaag cgtcgtagct tcgaacgttg
     1021 ttgcgaagct cccaggcccg tggcatcagg agtcggtggc tccaacgctc tcccggccag
     1081 gagcgaagat cgtcgacggc ctgcgacgca gcggtctggt cgaggtggcc gcggcgcagg
     1141 tgagtctgga tcaccgccag tacctcggcg tcgatgaggt gcggagcgct gagcgcttcg
     1201 ccggcggcga gcaaggttcg caggtcacga ctccgagggg tgtccgctac gacctcgaac
     1261 agcacgcttg cgtcaacgac gatcacggaa ccggagctgc ggagtcttga ggctcatgtt
     1321 tggtcgccgg gccacggccc tcgaagttcg tcgagggcgg ccaggacgtc atgtgtctgg
     1381 atgtcgcagg gccgtcgctc ggtccgcttc agccattcgg tgagcgacgg tctcgcagcg
     1441 atccgtgtga tctcgcgtcg caacagctca ggaaccgaca tctgcaattc ggccgcctgt
     1501 cgggcgagcg ccgcgtacac ctcatcgtcg aggtctcgga cttggacggt cttcgacaca
     1561 actgcatctt aacatgcaaa attgcatctc caatcggtgg ttcgggccac gaaggcgtcg
     1621 accacgccgt catcgacggt gtcggacgca gcgctgagcc ggagggttct cggctcagtc
     1681 gagcagtccg ccgtcggtga gcgcccgctc gatcgcacga cgttgaggat gttcggcgag
     1741 ctcgttcagc aggttcgcgg caagttgttt cgctcggtcg gttgggacgt gctcaccagt
     1801 ggaggtcgtg aacgcaaccg gcaacatgac cagaggaaga tcgttgaccc cgatgatgcg
     1861 cacaccggcg gcctcgtcga gggtgccggc acggaactgg tcgatcaact cggcgatcct
     1921 gcccgaatcg aaccagtcgc gacgtgactt cgggcgctgc gaaccggcgt ctcggaggtc
     1981 gatgaccacc gcagacgcgg tccggttggt tccaagcacc gcgatcagcg ggtcgaaacg
     2041 actccccgtc ttcaccgcag ccggcaacaa caccagcgcc tccagacgtc cgaacccgag
     2101 ttcgagagcg gatccccgga aggtgctgcc ccagtcgggc aggatcgccg atgtcgttgc
     2161 gatgacggca cgccctcccg gccgcagcgt ctcgttgacc gtcctgatcc accggaacag
     2221 gtcgcgtttg ccagccgtcg tcggcaggtc gctgagtatg acgtcagcgg ttccggcgtg
     2281 cccggtttgc ttcaaggcat cgccaacccg aagctcgatc ggcagtgacc gcagccagaa
     2341 ccggatccgg gtcacccgcg cgacggcggg atccagttcg ttggcgcaga gcgcgatgtc
     2401 agccgtcacg ccatgacgtc gtgaggccca cagcagcaac tctccgatcc caacgcatgg
     2461 atcggagatg accattggcc cgtcgtgatg gccggctgaa gcgctcgccc ctaactcgac
     2521 gatcaggtcg gtgagctcgt gagacgtgat gttcgcctgg cctgagcggt tgacgccgct
     2581 gagcgactcg agcgcatcgt cgagtagcgg cgcgggatcg aggccggcgt cgagtccggc
     2641 gtcgaggcgg cgcacgagcg cggcgggcac cacggcgagc cgctgtgcga tggatccggt
     2701 ctcaccgacg aggtcgccat atccgtcgac gtctcgcagc gccgacaatg aggacttcaa
     2761 cgtccggacg agtttggaag cgctgctgtg ttcgtagacg gtgtgcagac gctgggcccc
     2821 cgctgagcgc gagcgctgca cggcgagcgc tatgagtacc gccgccaact cgaatgagtc
     2881 gttgtcgctc gacatgacga gaacggcgcg actccaggcc caccggtgga tcggccgaaa
     2941 ttcatcgcca tcgacacgtc gccgggtgac gaattcg
//
LOCUS       DAOOMA010000320         2966 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_40612_length_2966_cov_3.947784, whole genome shotgun sequence.
ACCESSION   DAOOMA010000320 DAOOMA010000000
VERSION     DAOOMA010000320.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 2966)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2966)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2966
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40612_length_2966_cov_3.947784"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..308
                     /locus_tag="PLP95_08905"
     CDS             <1..308
                     /locus_tag="PLP95_08905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HPB45957.1"
                     /translation="HDNACTLLNLRAANRAVAGGEPGPEGAITKLVLSENGHEATAIF
                     TALTGPDAAFNEGPGEMSNMLALWHRGLSIAGGTSEIKRNQIGERILGLPRDPLISN"
     gene            538..1641
                     /locus_tag="PLP95_08910"
     CDS             538..1641
                     /locus_tag="PLP95_08910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB45958.1"
                     /translation="MADFIMVTVVVLRLVVPLFIPKFPLPAIVLALIIDGVDQTIFQI
                     FTDSDLPGYQSYDKALDVYYLAIAYLSTMRNWREPVAFDIARFLFLFRLVGVAFFEAT
                     QWRWILMVFPNTFEYYFIAYEAIRTRWNPFVLKSRQVAGLAAAIWVFIKLPQEWWIHV
                     AQFDVTDTLAEYPIGWLVIFGGLGLIGFVVWSLRGRIPAPDWPFTVRVDDHLQLSDYE
                     GRGRERFFSAILVEKIMLCALIAVIFSQVIPGVQATDTQLAIGVTVLVVANTLISQVI
                     RRFGKVWASSFTQFLATLAINIAIVWVDARFGGDSRVYGLRWSTVFYMVMLSFLMALF
                     DRYRLSRDPQERIDVMRTLRSGEITPPLARRPS"
     gene            1664..>2966
                     /locus_tag="PLP95_08915"
     CDS             1664..>2966
                     /locus_tag="PLP95_08915"
                     /inference="COORDINATES: protein motif:HMM:NF040521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C45 family autoproteolytic
                     acyltransferase/hydrolase"
                     /protein_id="HPB45959.1"
                     /translation="MDHERSTDHKLSTAYALATANVLPLRVYRLRGTQVEMGRQFGQL
                     AATDGGFQAAIDLYAERPTMAATMIAAGMPHRQRRTVERIVDTVFARQRRRLHRIRTG
                     HFADYAQRTAAGFAELGLPADRVSATFVMEALQNMVGVVGRTSMFTKPLQVAAVPMCS
                     AVAVWGAATSDGALRHARNFDFPGGSIWDRAPAVVFCDPDDGLRYGFVTTRGLDAPGI
                     TCFNEAGLTLSVHTRFHREVGWSGPSVIDLGHEIIRTCDTLDSAIETGRRLGATSTWG
                     LLISSASQNRAAVLETTAKRTAVTLSDSADTHLTCTNRYLSSELRPGEVVASPDFATD
                     SDDRQCQLERFVEDHRGSIDSAALQEVLGDLGAIDQTDLSDDIVRLCGHSVVAHHSVA
                     SIVSNPQARSIDVSVGATPTGFGPYATVPWSWDGPVGVVGVV"
BASE COUNT          483 a         1029 c          918 g          536 t
ORIGIN      
        1 cccacgacaa cgcctgcacc ctgttgaacc ttcgcgctgc caaccgtgcg gtcgccggcg
       61 gcgaaccggg ccccgaaggc gcgatcacca agctggtact cagcgagaac ggccacgagg
      121 ccaccgccat cttcaccgcc ctcaccggtc ccgacgccgc gttcaacgag ggccccggcg
      181 agatgtcgaa catgttggcg ttgtggcacc gcggcctgtc gatcgccggc ggaacctccg
      241 agatcaagcg caaccagatc ggcgaacgca tcctcggact cccccgcgac cccctcatca
      301 gcaactgagc gagcacggta gaccggtcgc gcgactgtcg ggcgtcgacg agcggacgtc
      361 tcgactcacg tcagtatcaa cgcgcgcgcc gagcgttcag cgcactcgtc gactcactgg
      421 tgatcgtcga ggtgaccgaa gcgctcatcg ccgacgcggc cgacctggcc gagtcccagt
      481 cgttgagggg ctacgacgcg gtccacctgc cctagtgtgg cgggccaagg agggccagtg
      541 gccgatttca tcatggtgac cgtcgttgtc ttgcgactcg tcgtcccact gttcattccg
      601 aaatttccgc tgccagcaat cgtgctggcg ctcatcatcg acggtgtcga ccagacgatc
      661 tttcagatct tcaccgacag cgatctaccg ggataccagt cctatgacaa ggccctcgac
      721 gtctactacc tagcgatcgc gtacttgtcg acgatgcgca actggcgcga accggtggcc
      781 ttcgacatcg cccggttcct ctttttattt cgtctcgtag gcgtcgcgtt cttcgaagcc
      841 acccaatggc gttggatcct catggtgttc ccgaacacct tcgagtacta cttcatcgcg
      901 tacgaggcca tccgtacccg ctggaacccg ttcgtcctga agtcccgcca agtcgccggc
      961 ctcgctgcgg ccatctgggt gttcatcaag ttgccccaag aatggtggat ccacgtcgct
     1021 cagttcgacg tgaccgacac cctcgccgag tatccgatcg ggtggttggt catcttcggc
     1081 gggctcggcc tcatcgggtt cgtggtctgg tcgctgcggg ggcgaatccc cgcacccgat
     1141 tggcctttca ccgtgcgcgt cgacgatcac ctgcaactga gcgactacga gggccgcggt
     1201 cgcgagcgtt tcttctcggc gatcctcgtg gaaaagatca tgttgtgcgc cttgatcgcc
     1261 gtgattttct cccaggtcat tcccggggta caggccaccg acactcaact cgccatcggc
     1321 gtgacagtcc tggtcgtcgc caacacgctc atcagccagg tcatccggcg tttcggcaag
     1381 gtgtgggcga gttcgttcac ccagttccta gcgacgctcg ccatcaacat cgcaatcgtg
     1441 tgggtggacg cccgattcgg cggcgactcg cgggtctacg ggctgcggtg gtcgacggtg
     1501 ttctacatgg tgatgttgag cttcctcatg gcgctgttcg accgctaccg gctgagccgc
     1561 gacccccagg aacggatcga cgtgatgcgg acgctgcgga gcggcgaaat cactcccccg
     1621 ctcgcgcgcc ggccgtcata gcgcccgatc tcacgagcgc cgcatggatc acgaacgcag
     1681 cacggatcac aaactcagca cggcgtacgc gctggccacc gccaacgtgt tgccgttgcg
     1741 ggtgtaccgc ctgcggggca ctcaggtcga gatggggcga cagttcggcc aactcgccgc
     1801 gaccgacggc gggtttcagg ccgcgatcga tctctacgcc gagcgtccga ccatggcggc
     1861 cacgatgatc gccgcgggaa tgccgcatcg acagcgccgt accgtcgaac ggatcgtcga
     1921 cacggtgttc gcccggcaac gtcgaaggct tcaccggatc cgcaccgggc acttcgccga
     1981 ctacgcgcaa cgcacggcgg cgggtttcgc cgaactcggc ctgccggcgg accgggtgag
     2041 cgcgaccttc gtgatggagg ccctgcagaa catggtcggc gtcgtcggcc gcacctcgat
     2101 gttcaccaag cccttgcagg tggcggcggt accgatgtgt tcggcggtgg ccgtgtgggg
     2161 cgcagcgacc tccgacgggg cgttgcgcca tgcccggaac ttcgactttc ccggaggctc
     2221 gatctgggac cgtgccccgg ccgtggtgtt ctgcgatccc gatgacgggt tgcgctacgg
     2281 attcgtcacc acgcggggcc tcgacgctcc gggcatcacc tgtttcaacg aggccgggct
     2341 gaccctgagc gtccacaccc gattccaccg cgaggtcggc tggtcgggcc cgtcggtgat
     2401 cgacctcggc cacgagatca tccgcacctg cgacaccctc gactcggcga tcgagacggg
     2461 tcggcgactc ggcgccacct cgacctgggg actgctcatc tcctccgcgt cgcagaaccg
     2521 ggccgctgtg ctcgaaacca cggccaaacg caccgccgtc accctcagcg actccgccga
     2581 cacccacctg acctgcacca atcgctacct gagcagcgag ttgcgccccg gagaggtcgt
     2641 cgcctccccc gacttcgcca ccgattccga cgaccgccag tgccaactcg agcgattcgt
     2701 cgaagaccat cgcggttcga tcgactcggc cgcgctgcag gaggtgttgg gcgacctcgg
     2761 ggcgatcgat cagaccgacc tgagcgacga catcgtgcgc ctgtgcggcc acagcgtggt
     2821 ggcccaccac tcggtggcct cgatcgtgtc gaatccgcaa gcccggtcga tcgatgtctc
     2881 ggtcggcgca accccgaccg ggttcggtcc gtacgcgacc gtgccgtgga gttgggacgg
     2941 gcccgtcggc gtcgtgggag tcgtca
//
LOCUS       DAOOMA010000321         2964 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_40657_length_2964_cov_2.764180, whole genome shotgun sequence.
ACCESSION   DAOOMA010000321 DAOOMA010000000
VERSION     DAOOMA010000321.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 2964)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2964)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2964
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40657_length_2964_cov_2.764180"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..349
                     /locus_tag="PLP95_08920"
     CDS             <1..349
                     /locus_tag="PLP95_08920"
                     /inference="COORDINATES: protein motif:HMM:NF013630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HPB45960.1"
                     /translation="TAADAAQRRVARNDEALRRTYLRRRVALAVMVLVTVLFVAASLN
                     GGNAEATLDESVPSSSYVVRSGDTMWSIVTELGYTGDRREAVSVLAEANGGSSDVVAG
                     QRLTVPAILGSVR"
     gene            complement(377..1156)
                     /locus_tag="PLP95_08925"
     CDS             complement(377..1156)
                     /locus_tag="PLP95_08925"
                     /inference="COORDINATES: protein motif:HMM:NF013989.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HPB45961.1"
                     /translation="MHLLTRHGSPTLTSDLTDDAVIELVMAEARIRADGRPWVMCNMV
                     TSLDGAAAVQGLSGSLGGAGDKVIFAALRARSDVIVAGSATVLAERYRLPRAPHGVAG
                     DWRGAHGKAPRPRLCIVTSRGGVPDADRLIGELPSPTTDNPQFRPIIATTDAAPDLAD
                     ERFDVIRCGADSVDPTMLVDRLAALGCSTILCEGGPNLLGQFAAADLIDEWDFTIAAT
                     IVSGDAIRPVTGPDNSPTALGLDRAFIHHDGTLLLRYLRDE"
     gene            1441..2916
                     /locus_tag="PLP95_08930"
     CDS             1441..2916
                     /locus_tag="PLP95_08930"
                     /inference="COORDINATES: protein motif:HMM:NF014868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB45962.1"
                     /translation="MRTWIGGFVLLPVAIKAGAGELRPMGHAEGRALVTLSARVHTPR
                     SHVFDQNLVAESLHAVDEPLPYQCATWAHSRSPTMSLTEQHVGLGLRRVFTTEGVDPY
                     DTLEWGLRDARILDYRDGSVAFEQNGVEFPLSWSVNASNIVAQKYFRGTPGTPEREWS
                     LRQVIDRVADTITGWGDRDGYFGSDAEREIFRDELTHVLVTQKASFNSPVWFNIGVPN
                     VPNQASACFILSVDDSMDSILNWYREEGIIFKGGSGAGVNLSHIRASNELLGGGGTPS
                     GPVSFMRGADASAGTIKSGGKSRRAAKMVILDVDHPDIMEFVTCKSREEEKARVLRDA
                     GFDMDLDGADSYSIQYQNANNSVRVSDEFMAAVEADEQWTLTGRRTESANRTVSAREL
                     MRAIALAAWDCADPGIQYDSTINRWHTASVTGRINASNPCSEYMHIDNSACNLASLNL
                     LSFLGPDGVFDVERYEAAIEKAAVCNGCSTTRASAVTPNSR"
BASE COUNT          511 a          957 c          967 g          529 t
ORIGIN      
        1 cactgcggcc gatgccgctc agcgtcgcgt cgctcgcaac gacgaagcgt tgcgccgcac
       61 ctacctgcgt cggcgcgtgg ccctcgctgt catggtgttg gtgacggtgc tgttcgttgc
      121 cgcgtcgctc aacggcggca acgccgaagc gaccctcgac gagtcggtcc cctcctcgtc
      181 ctatgtcgtg cgttccggcg acacgatgtg gagcatcgtc accgagcttg gctacacggg
      241 tgatcgtcgc gaggcggtgt cggtgctcgc cgaggcgaac gggggcagtt ccgacgtcgt
      301 cgcgggccaa cgattgaccg ttccagccat cctcgggtcg gtacgctgaa ggggcgtcgt
      361 cgccgtccgg cagacgtcac tcgtcgcgca gatatcgcaa caggagggtg ccatcgtgat
      421 ggatgaaggc acgatccaaa cccaacgcgg tgggcgagtt gtccgggccg gtcaccgggc
      481 gtatggcgtc gcccgacacg atggtggcag cgatggtgaa atcccactcg tcgatcaggt
      541 cggcggcggc gaattgcccc aggaggttcg gcccgccctc gcacaagatg gtggagcatc
      601 ccagcgcggc aagtcgatct accagcatcg tcgggtccac cgagtcggct ccgcaccgga
      661 tcacatcgaa gcgttcgtca gcgaggtcgg gggcggcatc ggtggtggcg atgatcggac
      721 gaaactgcgg gttgtcggtc gtgggggatg gcagctcgcc gatcaggcgg tcagcgtcgg
      781 gcacaccgcc gcgagaggtg acgatgcaca gccgtgggcg cggggccttg ccgtgagctc
      841 cacgccagtc gcctgcgact ccgtgcggag cgcgcggcag tcggtagcgt tcggccaaca
      901 cggtggccga accggcgacg atgacgtcgc ttctggcccg aagcgccgcg aagatcacct
      961 tgtcgccggc gcctccgagc gatcccgaca gcccctgaac cgccgccgcc ccgtcgaggg
     1021 aggtgaccat gttgcacatg acccatggcc ggccgtcggc gcggatccgg gcctcggcca
     1081 tcaccaactc gatcaccgcg tcgtcggtga ggtcgctggt gagtgtgggc gaaccgtgac
     1141 gggtcagcag gtgcatgaga atttttctcc gagaagttgt ccccgaactc catccacagc
     1201 gggtggggac ggacacgaac aaactggtgc atcggtggcc ccggtgcggg gccagattct
     1261 tggctcgact gtaggtcagg gtggcggcgt cggcccgcgg gcccaggcac agggtgcgtc
     1321 gagcgagctg tctatcccca agcgatgtcg tttgttccgc ccatttccac agttattcac
     1381 acgttgtccc acttttgcct cacaggaccg ccttcctagg gtctgggcac ggaccgtggt
     1441 gtgcgcacct ggattggtgg cttcgtcttg ttgccagtgg cgatcaaggc gggtgctgga
     1501 gaacttcgac cgatgggtca cgccgaaggg cgagccttgg tcaccctctc tgcccgggtg
     1561 cacacgccgc gttcccatgt cttcgaccag aaccttgtcg ccgaatccct ccacgccgtc
     1621 gacgagccgc ttccgtatca gtgcgcaacg tgggcgcatt caaggagtcc gaccatgtcg
     1681 ctgaccgaac aacacgttgg gcttggcctt cgccgcgtct tcaccaccga aggagtcgac
     1741 ccctacgaca ccctcgagtg gggcctgcgc gacgctcgaa tcctcgacta ccgcgacggt
     1801 tcggtggcct tcgaacagaa cggcgtggag tttccgctgt cgtggtcggt caatgcctcc
     1861 aacatcgtcg cccagaagta cttccgcggc acccccggaa cccccgagcg cgagtggagc
     1921 cttcgccagg tcatcgaccg cgtcgccgac acgatcacgg gctggggaga ccgcgacggg
     1981 tacttcggtt ccgatgccga acgggagatc tttcgagacg aactgaccca cgtgctcgtc
     2041 acccagaagg cgtcgttcaa cagtcccgtc tggttcaaca tcggtgttcc gaacgtgcca
     2101 aatcaggcgt ccgcgtgctt catcttgtcg gtcgacgact cgatggattc catcctcaac
     2161 tggtatcggg aagagggcat catcttcaag ggcggttcgg gtgccggggt gaacctgtcg
     2221 cacatccggg cctcgaatga gctgttgggc ggaggaggca ctccgtccgg tccggtgagc
     2281 ttcatgcgcg gggccgacgc ctcggccgga accatcaagt ccgggggtaa gtcgcggcgg
     2341 gcggccaaaa tggtgatcct cgacgtcgat caccccgaca tcatggagtt cgtcacgtgt
     2401 aagtcgcgtg aggaggaaaa agctcgcgtg ctgcgcgacg ccggcttcga catggacctc
     2461 gacggtgccg actcctacag tatccaatac cagaacgcga acaactcggt gcgggtgagc
     2521 gacgagttca tggcagcggt ggaggccgac gaacagtgga ccctgaccgg gcgtcgcacc
     2581 gagtcggcca accgtaccgt gagcgcacgc gaactgatgc gtgccatcgc attggccgca
     2641 tgggactgcg ccgaccccgg cattcaatac gactcgacca tcaaccgttg gcacaccgcg
     2701 tcggtgacgg ggcggatcaa cgcctccaat ccctgttcgg agtacatgca catcgataac
     2761 tcggcgtgca atctggcgag tctgaacctg ctgtcgttcc tcggccccga cggggtcttc
     2821 gacgtcgaac gctacgaggc ggccatcgag aaagcagccg tatgcaacgg ctgctcgacg
     2881 acaagggcat cggccgtgac gcctaactca agatgaggcg catcaccccg tctgttgcga
     2941 tcctccatcc ctccctcccc cctc
//
LOCUS       DAOOMA010000322         2958 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_40763_length_2958_cov_3.778505, whole genome shotgun sequence.
ACCESSION   DAOOMA010000322 DAOOMA010000000
VERSION     DAOOMA010000322.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 2958)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2958)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2958
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40763_length_2958_cov_3.778505"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(200..559)
                     /locus_tag="PLP95_08935"
     CDS             complement(200..559)
                     /locus_tag="PLP95_08935"
                     /inference="COORDINATES: protein motif:HMM:NF016130.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF411 domain-containing protein"
                     /protein_id="HPB45963.1"
                     /translation="MYLRTHGATVEVTDDPDRASFREASGIADEVASCHTGEVDGYLI
                     EGHVPAEAIERLLAERPDAAVGLALPGMPADSPGMGGGDTSWAAQPVVLIGTSYPSQT
                     SPAEPLRRRVPGLKSRR"
     gene            873..1268
                     /locus_tag="PLP95_08940"
     CDS             873..1268
                     /locus_tag="PLP95_08940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HPB45964.1"
                     /translation="MMTGRGAQVASIEACDVRIVDPDKVATTREQLPSTAETDRLADW
                     FKVLGDATRARILYAVLEAGELCVCDLAATVDVPESTVSHALRWLRGAGVVRARRSGK
                     MMYYRLDDEHVRMLLDIGREHLRHRDGGC"
     gene            1268..2173
                     /locus_tag="PLP95_08945"
     CDS             1268..2173
                     /locus_tag="PLP95_08945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012224376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HPB45965.1"
                     /translation="MGHEHSHVDLRAGARHAGRLWWSFALVAGFLVVQVVVGLAANSL
                     ALLSDAGHMATDALGLGMALAAITAANRAIQAQHRTFGLYRLEILAALANAVLLFGVA
                     GYVLIEAVRRLDDPPDVASVPVLIVGIIGLVVNLIAFLLLRQGATESLNVRGAYFEVI
                     ADALGSVGVIVAAAVMWATGWGWVDPVIGAGIGLFILPRAWRLGRDAMRILVQAAPEG
                     VDLAAVTTALGGIDGVEDVHDLHVWTLTSEMDVLTAHLGIAERGDAQTVLQQARAVLA
                     ERFHLEHATLQVETAGDHSCEEMTW"
     gene            2167..2778
                     /locus_tag="PLP95_08950"
     CDS             2167..2778
                     /locus_tag="PLP95_08950"
                     /EC_number="2.1.1.100"
                     /EC_number="2.1.1.334"
                     /inference="COORDINATES: protein motif:HMM:NF016107.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HPB45966.1"
                     /translation="MVNGRALLALVLQLTFLAAAFGWRTTVHRRRTGDSGFRWQRRDR
                     VARVSGALFAVAILIGTTGVTLAAFSVTELWDPLAGSGAFVAGLVVFFAGCAVTLLAQ
                     SEMGASWRIGVDPSERTGLVTNGLFGWARNPIFTGMVTAAAGLTLLAPTVLTTAAVVM
                     LVAAVQIQVRSVEEPYLRVAMPDWLSYAGRVGRFVPGLGKFGS"
     gene            2778..>2958
                     /locus_tag="PLP95_08955"
     CDS             2778..>2958
                     /locus_tag="PLP95_08955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB45967.1"
                     /translation="MDACCGPAATAAEASETDSGRFEWRTIAAAVAAASWVAGVVAEV
                     ADAGSVATVAFVVAIV"
BASE COUNT          438 a          937 c         1014 g          569 t
ORIGIN      
        1 cgatcgccgg gctcgcaggt ctcgacccga taacgccact cgacagcacg gcggcccagg
       61 ctgtcggcct cgcggtggcg atcgccgtga tcatggcgac gaccgcagcg cagatgggtc
      121 gtggcgtatt gcgtcgatcc aaccgagcgc accggcctgg tcaccagcgg tgtgttcgcc
      181 ctggtccgca acccgatctt caccgcctac tcttgagtcc cggaacgcgc ctgcggaggg
      241 gctccgcggg cgatgtctgg ctggggtagg aggtgccgat caataccacg ggctgtgcgg
      301 cccagctggt gtcgccgccg cccattccag gcgaatcagc gggcatgccc gggagcgcca
      361 agcccaccgc tgcgtcgggt cgctcagcca gcagtcgttc gatcgcctct gcgggaacgt
      421 ggccttcgat caggtagccg tcgacctcgc cggtatggca tgacgcaact tcgtcggcga
      481 tgccgcttgc ttcacggaac gatgcgcgat cgggatcgtc ggtcacctcg accgtggcgc
      541 catgtgtgcg taggtacacg acccacgaag tgcaacagcc tcaaccgggg tcgcgacgaa
      601 cgtcgaactg caccccgtcg agatcggctg cgtcgtcgac cacggcaact tgcgtgcttt
      661 ctggtccatc tgctgtgtcg ctgccggcag aacagcccga gaccaacgca gcaatcccca
      721 acgccacgac acacgaaccc cggaacggct tcatcgactc gtaccgtacc gaggccgttg
      781 cgttcttccc agtgacgatg gtcatctggt gccaacggtg ccagagcgaa tcttccttgc
      841 ggattcatca tgtcatctgt acagtcatgc agatgatgac aggccgggga gctcaggttg
      901 cgtcgatcga ggcgtgcgat gttcgcatcg tcgaccccga caaggtcgca acgacgcggg
      961 agcagttgcc gtcgaccgcg gagaccgacc ggctggcgga ctggttcaaa gtgctcggcg
     1021 acgccactcg cgctcggatc ctgtacgccg tgctcgaggc cggcgaattg tgcgtgtgcg
     1081 acctggcggc caccgtcgac gtgccggagt cgacggtctc gcatgcgttg cgctggctgc
     1141 gcggcgccgg cgtagtgcgg gctcgacggt ccgggaagat gatgtactac cgcctcgacg
     1201 acgaacacgt ccggatgctg ctcgacatcg gccgcgagca cctccgtcac cgcgacggtg
     1261 gttgctgatg ggccacgaac acagccacgt cgacctgcgc gcgggcgccc gccacgccgg
     1321 caggttgtgg tggtccttcg cactcgtcgc aggatttctc gtcgtccagg tcgtggtcgg
     1381 gttggcggcg aactcgctgg cgctgctgtc cgacgccgga cacatggcca ccgacgcgct
     1441 cggtttgggg atggcgttgg cagcgatcac cgcagcaaac cgcgccatcc aggcacagca
     1501 ccgcacgttc ggtctgtacc gcttggagat cctcgctgcg ctcgccaacg ccgtgctgtt
     1561 gttcggcgtt gccggctacg tgctgatcga agcagtgcgc cggctggacg atccaccgga
     1621 tgtcgcgtcc gtgccggtgc tgatcgtcgg catcatcggc ctcgtggtga acctcattgc
     1681 gttcctcttg ttacgtcagg gagcaaccga gagccttaat gtgcgcggcg cctacttcga
     1741 ggtgatcgcc gacgctctcg ggtcggtcgg tgtgatcgtc gctgctgcgg tgatgtgggc
     1801 gaccgggtgg ggctgggttg acccggtgat cggcgccggt atcgggctgt tcatcctgcc
     1861 ccgggcatgg cgcctcggcc gcgacgcaat gcggatcctg gtgcaggccg cacccgaggg
     1921 cgtcgaccta gccgcggtga ccactgccct cggcggcatc gacggggtcg aagacgttca
     1981 cgacctgcac gtgtggacct tgaccagtga gatggatgtg ctcaccgcgc accttgggat
     2041 cgctgaaaga ggcgacgcac agacggtgct gcaacaggct cgcgccgttc ttgcggaacg
     2101 cttccatctg gagcacgcca cgttgcaggt cgagaccgcc ggcgatcatt cctgtgagga
     2161 gatgacatgg tgaacggcag agcgttgctg gcgctggtgt tgcagctcac atttctggcg
     2221 gctgcgttcg ggtggcgaac aaccgtgcat cgtcggcgca ccggtgactc ggggttccgt
     2281 tggcaacggc gtgatcgagt tgcgcgtgtc agcggtgcgc tgttcgccgt cgcgatcctg
     2341 atcggcacca ccggtgtgac ccttgccgcg ttctcggtca ccgagctgtg ggacccgctc
     2401 gccggaagcg gcgcgttcgt cgcaggtctg gtcgtgttct ttgctggatg tgctgtgact
     2461 ctgttggcgc agtcggagat gggggcgtcg tggcggattg gggtcgaccc gtccgaacgc
     2521 acgggactcg tcacgaatgg tctattcggg tgggcgcgca acccgatttt caccgggatg
     2581 gtgaccgctg ccgccgggtt gacgttgctc gccccgactg ttctcacgac cgcagccgtg
     2641 gtcatgctcg tcgcggcggt gcagatccag gtccgctcag tcgaggagcc ctacctccgt
     2701 gtcgcgatgc ctgactggtt gagctacgca ggtcgtgtcg gacgtttcgt gcccggactc
     2761 ggaaagttcg gaagttgatg gacgcttgtt gtgggccggc ggcgaccgcc gctgaggcat
     2821 ccgagaccga cagtggccga ttcgagtggc gcacgatcgc cgccgctgtc gctgctgcat
     2881 cgtgggtggc aggtgttgtc gccgaagtcg ccgatgctgg atctgtcgcg acggtcgcgt
     2941 tcgtcgttgc gatcgtcg
//
LOCUS       DAOOMA010000323         2958 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_40765_length_2958_cov_3.532208, whole genome shotgun sequence.
ACCESSION   DAOOMA010000323 DAOOMA010000000
VERSION     DAOOMA010000323.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 2958)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2958)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2958
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40765_length_2958_cov_3.532208"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..882)
                     /locus_tag="PLP95_08960"
     CDS             complement(<1..882)
                     /locus_tag="PLP95_08960"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HPB45968.1"
                     /translation="MSPDSSSIPSEAAASDTRVIWVLAGAGMTVSVMQTIVIPLIPSF
                     PTLFNTDAASASWVITATLLVAAVSTPVMGRLGDMFGKKRMLLICMGFLAGSAVVCAL
                     STSLPVVVFGRGVQGLGIGAIPLGISLMRDIVAPARLGSGVALMSSSLGAGGALALPV
                     AAFIADRWGWSALFWFAAALGSALGALVAVGVPESPVRRPGSVDLVGVGGLAVGMGSL
                     LLAAGEPGQRFALPNARIMVHQPSGGFQGQATDILIHAREIEALRRRLNEIYVKHTGR
                     DIAAIEQALERDNFMTAD"
     gene            1147..2007
                     /locus_tag="PLP95_08965"
     CDS             1147..2007
                     /locus_tag="PLP95_08965"
                     /inference="COORDINATES: protein motif:HMM:NF014708.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HPB45969.1"
                     /translation="MEIHEGSRAAIGAIDVVRVLPRRTRRTVGPWCFLDHMGPSTVSG
                     APGLGVGPHPHIGLQTVTWLLEGAVLHRDSLGTEQLIRPGQLNLMTAGRGVSHSEETA
                     GYYDGALHGVQLWVALPEAGRAIEPAFEHHGELPVVEMGDARATVLMGTFAGATSPAR
                     RDGAIVGVELAMAPGRCEFALDPTFEHALTVVDGSHVVVDGADLAADRLAVFGPGRTS
                     LSVQAGAPARLMLIGGPPRVDPIVMWWNYVAPSRDEIIEAHLQWTAEDARFGTVASPL
                     PRVLVDGPPW"
     gene            2004..2780
                     /locus_tag="PLP95_08970"
     CDS             2004..2780
                     /locus_tag="PLP95_08970"
                     /inference="COORDINATES: protein motif:HMM:NF017932.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M90 family metallopeptidase"
                     /protein_id="HPB45970.1"
                     /translation="MIGRGRRRRQRWSAGFDPRWRELVSRRLNAWNALDDEERERLEF
                     LTMALMFDKRWEAANGFELTDEIQVTIASQAALLALGLPDDVYRKVRTILVHPTTLVM
                     RGEHSQVPGIVSNADMAVLGLAEHTGTVMVAWDSVLSDARRPGNGHNVVIHEFAHQLD
                     MLDGTTDGTPPLATTEQFQRWVDVCTDAYRQVSAGHGGRSLRSYAGVNPGEFFAVATE
                     AFFDDARELRREHRELYEVLSEYYRQDPASRTVRQQRPIA"
BASE COUNT          544 a         1020 c          956 g          438 t
ORIGIN      
        1 gtcggcggtc atgaaattgt cacgctcaag cgcctgctcg atggcggcga tatcccgccc
       61 cgtgtgcttc acgtaaattt cattgagacg gcgacgcagg gcttcgatct cgcgggcatg
      121 gatcaggata tcagtcgcct gtccctgaaa accgccagat ggctgatgca ccatgattcg
      181 cgcgttcgga agcgcaaagc gctgaccggg ctctccggcc gccagcagca gcgatcccat
      241 tccgacggcc aggccgccga cgccgacgag atcgaccgac cccggtcgac gcacgggcga
      301 ctccgggacg ccgactgcga ccaacgctcc caacgccgac cccaacgccg cggcgaacca
      361 gaacagcgcc gaccaccccc accgatcggc gatgaacgcc gcgaccggca gggcgagcgc
      421 tccgccggcc ccgagcgagg aactcatgag ggcaactccc gacccgagtc gtgcgggggc
      481 gacgatgtcg cgcatcaggc tgattccgag cgggatcgcc ccgataccga gcccttgcac
      541 gccacggccg aacaccacca ccggcaacga ggtgctgagc gcacatacca ccgccgaacc
      601 cgcgagaaac cccatgcaga tcagcagcat ccgcttcttg ccgaacatgt cgccgagacg
      661 ccccatcacg ggggtcgaaa cggctgcgac aagcagggtc gcggtaatga cccaactggc
      721 gcttgccgcg tcggtgttga acaacgtcgg aaacgacgga atgagcggaa tgacgatcgt
      781 ctgcatcacc gaaacggtca ttccggctcc cgcaagcacc cagatgaccc gggtgtccga
      841 agcggccgcc tctgacggga tggatgagga atcgggggac atggcactca cgatcagacc
      901 cgggactctc ggcgcgatag cgcgcgatcg ggcagcgttc atcctgacgg agccgatgcg
      961 tggcgtcaca ttggacacga tggcagcggg tcgctacccg gcccatgcac gggtgcgccg
     1021 cagctacccg acccattcac gttctgtagg taattccggt ggccatgacc accggaatta
     1081 cctacagaac gccagggcgg cctagggcgg ggagctaggc cggcgttcac cgagtagaac
     1141 gagtcgatgg agattcacga aggcagtcgc gccgcgatcg gcgccatcga tgtcgtgcgg
     1201 gtcctgcccc gacggacccg gagaaccgtt gggccgtggt gctttctcga ccacatgggt
     1261 ccgtcgacgg tgagcggcgc gcccggcctg ggggtcgggc cgcaccccca catcgggcta
     1321 cagaccgtga cctggctgct cgagggcgcc gtgttgcacc gcgacagcct cggaaccgaa
     1381 caactcatcc gaccggggca gctcaacctc atgaccgccg ggcggggcgt ctcgcattca
     1441 gaagagaccg ccggctacta cgacggagcg ctgcacgggg tacagctctg ggttgcgctg
     1501 cccgaggccg gccgagcgat cgaaccggcc tttgaacacc atggcgagct gcccgtggtc
     1561 gagatgggcg acgcacgagc gacggttctc atggggacct tcgccggggc gacctcccca
     1621 gcacggcgcg acggtgccat cgtcggagtc gaactcgcga tggcacccgg gcggtgtgaa
     1681 ttcgcactcg atccgacctt cgaacacgcg ctcaccgtcg tcgacggcag ccacgtcgtc
     1741 gtcgacgggg ccgacctcgc cgccgaccga ctcgcggtgt tcggaccggg gcgaacgagt
     1801 ttgagcgtgc aagccggtgc gccggcccgc ctgatgttga tcggcggccc gcctcgtgtc
     1861 gatccgatcg tcatgtggtg gaactacgtc gccccctccc gtgacgagat catcgaggcc
     1921 catctgcagt ggaccgccga agacgcacgc ttcggcaccg tggcgtcgcc gttaccccgg
     1981 gtgctcgtcg acggaccgcc ctggtgatcg gccgaggacg gcgccgacgc caacgctggt
     2041 cggcggggtt cgatccacgg tggcgtgagc tcgtctccag acgcctcaac gcctggaatg
     2101 cgctggacga tgaggagcgc gagcgcctcg agttcctcac gatggcgctc atgttcgaca
     2161 agaggtggga ggccgcgaac ggcttcgagc tcaccgacga gatccaggtc acgatcgcgt
     2221 ctcaggcggc gttgctcgcc cttgggcttc ctgacgacgt ctaccgcaag gttcgaacga
     2281 tcctcgttca cccgaccacc ctggtgatgc gcggcgaaca ctcgcaggtc ccgggcatcg
     2341 tcagcaatgc cgacatggcg gtcctcggcc tcgcggagca caccggaacg gtgatggtcg
     2401 cgtgggactc ggtgctgtcc gatgcccgcc gtcccggcaa cgggcacaac gtcgtcatcc
     2461 acgagttcgc ccatcaactc gacatgttgg acggcacgac cgacggcacc ccaccgctgg
     2521 cgacgaccga acagttccaa cgctgggtcg atgtgtgcac cgacgcgtat cggcaagtct
     2581 ccgccggcca tggcgggcga tcgttgcggt cctacgccgg cgtcaacccc ggagagttct
     2641 tcgccgtcgc caccgaagcg ttcttcgacg acgcccgcga actgcgccgc gagcaccgtg
     2701 agctctacga agtgttgagc gagtactacc gccaagaccc ggcatcgcga acggttcgac
     2761 agcaacgacc gatagcgtga cgcctcgtga aggcccttcc ccgacctcgc ccggctcgtg
     2821 aactcgtcgc acagatcccc gaacgggcga acgtcgtgtt gggtatgtcc aacggcgagg
     2881 cccacgggct gaccgacgcc ctcgaacacg cggccgccga tggagaactc catcgagccc
     2941 agagagctac cagtcagg
//
LOCUS       DAOOMA010000324         2956 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_40798_length_2956_cov_3.548776, whole genome shotgun sequence.
ACCESSION   DAOOMA010000324 DAOOMA010000000
VERSION     DAOOMA010000324.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 2956)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2956)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2956
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40798_length_2956_cov_3.548776"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(37..567)
                     /locus_tag="PLP95_08975"
     CDS             complement(37..567)
                     /locus_tag="PLP95_08975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB45971.1"
                     /translation="MGPLTLPGYGMNHNDEFWFSWSPQRKFRFARTMVVLFIVVALLA
                     RCALGGDGGEGGSDGASTTTTPTASTTTLSPTPTSVGAVDVATKLPAARYISSVARAG
                     DKVMIVAGLDEKKNSTDTIWLFDPEAGTIADVGKFGSGSCRRGGAAGRSRPHHGRGQG
                     RFGLQHGVRGGCRRQR"
     gene            774..2372
                     /locus_tag="PLP95_08980"
     CDS             774..2372
                     /locus_tag="PLP95_08980"
                     /inference="COORDINATES: protein motif:HMM:TIGR02669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIID/LytB domain-containing protein"
                     /protein_id="HPB45972.1"
                     /translation="MLRNPRRVVVLVVCAALVSALGCQPEVPAPDATFTVSGGGYGHG
                     VGMSQYGAYGRALAGHDVAQILDAYYPGTAIEPRSLGNIRVHIGDAQATELTNPTGNI
                     VITAESGASSVIAGPNERLAVRRNGASVQVQVVAPNVGGTLTFPGAQHVYFSSAGDES
                     IRVAATGRSYRFGRLDVSPNGVGLRMIVRDLSMDQYLYGLGEMPASWPLEALKAQAIA
                     GRSYAARALAAPKNAEFDLYSTTVDQAYVGTSVTDGSSYGANWKAAVDATSGRVLTHG
                     AAVAQTYYSSSNGGFSERSDYVWASSLPYLIAAADPFDAVAANKNSRWTRTFTATELG
                     AWVENAGRGSIGAVTRVELSGNLGASGRAERATVKLVGVTASATMTGAQFRTMINNRA
                     GASRQLLSTRFTVSPTPGAAAPDLPQPGPPPATNPGAAGDTTPPNLAVWHSDPMTFGA
                     AGQVCSYVTSDEPTIYALSINLRGTEVKSPVGGVSPDRPALMCIAIPARLRPTKPTLV
                     TFTAVGIDAAVNYRFVVKQVTVQR"
     gene            complement(2396..>2956)
                     /locus_tag="PLP95_08985"
     CDS             complement(2396..>2956)
                     /locus_tag="PLP95_08985"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family protein"
                     /protein_id="HPB45973.1"
                     /translation="LILASASPRRSELLAGAGYEFDIVAADIDETPFEYEKPEAYVER
                     LAAAKAAAVAAQHPDAVVVGADTIVVLGNELVGKPRDDADAAAILRRLSGAAHHVLTG
                     IAVASPTLNTALSVESAVWFRELDDDEIDAYVATGEPLDKAGAYAIQGGGADFVSEIE
                     GAWDNIVGLPVSWLEGLLLPAGVTPR"
BASE COUNT          459 a          972 c         1042 g          483 t
ORIGIN      
        1 gccccggcgc gcgcagcggg aaggttgccg attccttcaa cgttgccgtc ggcatccacc
       61 gcgtacaccg tgctgtagac cgaatcgccc ttggccccgc ccatgatgag ggcgcgatcg
      121 cccagcggcg ccgccgcggc ggcatgaacc ggagccgaac ttgccgacgt cggcgatggt
      181 cccggcctcg gggtcgaaca gccatatggt gtcggtggag ttcttctttt cgtccaggcc
      241 ggccacgatc atcaccttgt ccccagcgcg ggcgacgctc gaaatgtaac gagcggcggg
      301 gagtttggtg gccacgtcga ctgcgccgac cgaggtcggg gtcggagaca gggtggtggt
      361 gctcgcggtt ggcgtcgtgg tggtcgatgc gccgtcgctc ccaccctcgc cgccgtcccc
      421 gccgagggcg caccgagcga gcagcgcaac gacgatgaag agcaccacca tcgtgcgggc
      481 gaatcggaac ttgcgttgtg ggctccacga gaaccagaac tcgtcgttgt ggttcatgcc
      541 gtagccgggc agcgtgagtg gacccacggc gcctggggca cgccgcgccg gtcgacgcgg
      601 tgctcggcgc ggcggtcggt tcaggggtcg ctgtggcatg ggtcgattgt tgcaggtgat
      661 ctgggcgccg cgtaggccag cgcggcggtg cgacggagtc gatcggctca ccttggtggt
      721 tcaaaggcac tgcggggcgt ggaatttcga cgatgctgcg taccctcgat cacatgctga
      781 ggaaccctcg acgtgtcgtc gtcctggtgg tgtgcgcagc gctggtttcg gcgcttggct
      841 gccaaccgga ggtgccggcg ccggacgcaa ccttcaccgt gtcgggcggc ggatacggcc
      901 acggtgtcgg gatgagccag tacggggcct acgggcgcgc gctggcgggt cacgacgtgg
      961 cgcagatcct cgacgcctac tatccgggca ctgcgatcga gccgaggtcg ttgggcaata
     1021 tccgggttca catcggtgat gcgcaggcga cggagctgac gaacccgacc ggcaacatcg
     1081 tcatcaccgc cgaatcgggt gcctccagcg tgatcgccgg gccgaacgaa cgtctcgcgg
     1141 tacgtcgtaa cggcgcgtcg gtgcaggtac aggtggtcgc acccaacgtc ggaggaacac
     1201 tcacgtttcc gggggcgcaa catgtgtact tctcctcggc cggcgacgag tcgattcgcg
     1261 tcgcggccac cggacggagc tatcggttcg gccgcctcga tgtgtcgccg aacggggtcg
     1321 ggttgcggat gatcgttcgc gacctgtcga tggatcagta cctctacggg ctcggcgaga
     1381 tgccggcgag ttggccactc gaggcgctca aggcccaggc gatcgccggg cgcagctacg
     1441 cggcgcgggc gttggccgct ccgaagaacg ccgagttcga cctgtactcg accaccgtcg
     1501 accaggcgta tgtgggtacc tcggtgaccg atggatcgtc gtacggggcg aactggaagg
     1561 cggcggtgga cgcaacctcg ggcagggtgc tgacccatgg cgccgccgtc gctcagacgt
     1621 actactcctc gtccaacggc ggcttcagcg agcgttccga ctatgtctgg gcgtcgtcgt
     1681 tgccctatct gatcgccgcg gcggacccgt tcgacgccgt ggctgcgaac aagaactcgc
     1741 gctggacacg aaccttcacc gctaccgagt tgggcgcctg ggtggaaaac gccggtcgcg
     1801 ggtcgatcgg tgccgtaacc cgagttgaac tgtccggcaa cctcggggcc tccggacgag
     1861 ctgagcgtgc gacggtgaaa ctcgtcggag tcaccgcgtc ggcgaccatg accggcgcgc
     1921 agttccgcac gatgatcaac aaccgggcgg gcgcgtctcg ccagctgctg tcgacacggt
     1981 tcacggtgag cccgacgccc ggcgccgccg caccggacct gccgcaacca gggccaccgc
     2041 cggcgaccaa tccgggcgct gcgggtgaca cgacacctcc gaatctcgcg gtctggcaca
     2101 gcgacccgat gacgttcggc gctgcgggcc aggtctgcag ctacgtgacg agtgacgagc
     2161 cgacgatcta cgcgctgtcc atcaaccttc ggggaacgga ggtgaagagc ccggtgggcg
     2221 gcgtttcccc cgaccgtccg gcgctcatgt gcatcgcgat tcccgcgcgg ctccggccga
     2281 cgaagcccac gctggtgacc ttcaccgccg tcggaatcga cgccgcggtg aactaccggt
     2341 tcgtcgtgaa gcaggtgacg gttcagcgct gagccgacgg gtccggtagg gatcgtcagc
     2401 gcggcgtgac gcctgccggg agcagcagcc cctcgagcca gctcacgggg aggccgacga
     2461 tgttgtccca cgcaccctcg atctcggaca cgaagtcggc acctccgccc tgaatcgcgt
     2521 aggcgccggc cttgtccagg ggttcgccgg tggccacgta ggcgtcgatc tcgtcgtcgt
     2581 cgagttcgcg gaaccacacc gcggactcga ccgagagcgc cgtgttcagc gtcggtgacg
     2641 cgacggcgat gccggtgagg acgtgatggg cggcgccgga cagccgtcgc aggatcgccg
     2701 ccgcgtcggc atcgtcgcga ggcttgccga ccaattcgtt tcccagcacc acgatcgtgt
     2761 cggctcccac gaccacggca tcggggtgct gtgcggcgac cgcagccgcc ttggctgccg
     2821 ccagccgctc cacgtaggcc tcgggcttct cgtactcgaa cggggtctcg tcgatatcgg
     2881 cagcgacaat gtcgaattcg taaccggcgc ccgccagcag ttcgctgcgc cgcggcgagg
     2941 ccgaagcgag gatcaa
//
LOCUS       DAOOMA010000325         2954 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_40837_length_2954_cov_3.713349, whole genome shotgun sequence.
ACCESSION   DAOOMA010000325 DAOOMA010000000
VERSION     DAOOMA010000325.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 2954)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2954)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2954
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40837_length_2954_cov_3.713349"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            325..1314
                     /locus_tag="PLP95_08990"
     CDS             325..1314
                     /locus_tag="PLP95_08990"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004024 - alcohol dehydrogenase activity,
                     zinc-dependent [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MDR family oxidoreductase"
                     /protein_id="HPB45974.1"
                     /translation="MASFNAFLLSKTDDGQSLAWTEVDETDLMEGDVTVEVSHSSLNF
                     KDGLAITGSSPVVRRWPMIPGIDFVGTVRDSSNSAFEEGDTVVLNGWGVGETHLGGYA
                     ELARVPGEWLVALPEGITPSQSMAIGTAGYTAMLCVMALESRGVRPASGPVLVTGAAG
                     GVGSVAIAILAARGYEVIASSGRAESEGEYLMSLGATQIIDRSELSEPGRPMGKERWG
                     AAVDSVGSHTLANVLAQTRYGGTVAACGLAQGMDLLTSVAPFILRGVSLLGVDSVMAP
                     LELRHEAWRRLATDLPLDLLDSLTVTHPLSDAPALAQDILDGKVRGRVVFEVA"
     gene            complement(1602..1928)
                     /locus_tag="PLP95_08995"
     CDS             complement(1602..1928)
                     /locus_tag="PLP95_08995"
                     /inference="COORDINATES: protein motif:HMM:NF014505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HPB45975.1"
                     /translation="MTAFPRRGDLWWCEHPEIGRRPVVVLSRDVAIPRLGRALIAPCT
                     TTVRGLPSEIVLEPGDDPIERRCAVNLDSVESVSLALLVQRIGSLSGDRMHGICEALA
                     VATDCR"
     gene            complement(1925..2179)
                     /locus_tag="PLP95_09000"
     CDS             complement(1925..2179)
                     /locus_tag="PLP95_09000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012222944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin MazE5"
                     /protein_id="HPB45976.1"
                     /translation="MPYLRMARIRISTTVDADLLTDARRLRSDSTDSKLIDAALAALL
                     AAERRATIDAEYAKAYENHPIGEPDDWGDLESFRNAVAST"
     gene            2592..>2954
                     /locus_tag="PLP95_09005"
     CDS             2592..>2954
                     /locus_tag="PLP95_09005"
                     /inference="COORDINATES: protein motif:HMM:NF013584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HPB45977.1"
                     /translation="MSERTASDRVASERVASERVHAFTDDALGDDDAVALAERVRSGE
                     VHPRELLEAAIARAAMVDPTLRAVETPMYDRPRTGAGSDAPFFGVPTYLKDNTEVLGV
                     PTGNGSEAYVAKPAQKDAP"
BASE COUNT          494 a          918 c         1009 g          533 t
ORIGIN      
        1 cgcgggcacg tagctcgtca ggccggtcgc ctggtcaacg agacaggcgt tcgccgatcg
       61 attcatgagt ggcagcgcct tttccggatc gacgacgcgg tagcgaaagt caagcatgta
      121 cccggccgcc gagcgccgca cgccgagcac ttcgatcccc cagccgctgg ccggacccgc
      181 aaggtcgttg gcacccggcg ccgccctgac cgccgggtcg ggggccgtcg gctccgtgcg
      241 ccgcggggct gttgagctcg ctcatggaaa tcatcgtacc gaccggtaca actttggttc
      301 tcggttttcg ctaacgtgct tcccgtggct tcattcaacg cgtttctcct gtccaagacc
      361 gacgacggcc agtccctcgc atggaccgag gtcgacgaga ccgacctgat ggaaggcgac
      421 gtcaccgtcg aggtgtcgca ctcgtcgctc aacttcaaag acggtctggc gatcaccgga
      481 tcgtcgccgg tcgttcgtcg ctggccgatg attcccggca tcgacttcgt cggaaccgtg
      541 cgcgactcgt ccaactcggc attcgaggag ggcgacacgg tcgtgctgaa cggttggggt
      601 gtcggcgaga cccacctggg cggctacgcg gagttggcga gagtgccggg tgagtggctc
      661 gtcgcactgc ccgagggcat caccccgagc cagtcgatgg cgatcggcac tgccggttac
      721 accgcgatgt tgtgtgtcat ggcactggag agccgcgggg tgcgaccagc gtcgggacct
      781 gtcctggtta ccggtgctgc ggggggtgtc ggcagcgtgg cgatcgccat cctcgcggcc
      841 cgaggctatg aggtcatcgc atcgagtggc cgtgccgaat ccgagggcga atacctgatg
      901 tcgctcggtg cgacacagat catcgaccgt tccgaactct ccgagcctgg gcgtccgatg
      961 ggtaaggaac gctggggggc cgcggtcgac agcgtcggca gtcacaccct cgcgaacgtg
     1021 ttggcacaga cccgctacgg cggcacggtc gcggcgtgcg ggttggcgca gggcatggac
     1081 ttgctcacat cggtggcgcc gttcatcctg cgcggcgtca gcctgctcgg tgtcgacagc
     1141 gtgatggccc cgcttgagtt gcgccacgag gcctggcgac gtctggcgac ggaccttccc
     1201 ctcgatctgc tcgactcgct gaccgtcacc catccgctgt ccgacgctcc ggcgttggcc
     1261 caggacattc tcgatggcaa ggtgcgcgga cgcgtcgtgt tcgaagtcgc ctgattcgtt
     1321 ggccggcgcc ggagcggatt gattggatcg tcggcgcttg tgtggcttga cggtgcggac
     1381 tgaagcggcg attaccggtt ccgtcgaact gacggggctc cttacactcg ggtcatgtcc
     1441 gtggcgatcg agctgtcgga cgaggttctg cgccgactcg aagcggccgc cgcggcgcta
     1501 catgccccac aggcaggcct tggcaaaagc gatgctctcg cgaacgttct cgcgctgaac
     1561 caacttgggt gcgcggcggg agggggtgag gtgggcccgg ctcaccggca atcggtggcg
     1621 actgcgagtg cctcgcagat tccgtgcatt cggtcgccgc tgagcgagcc aatccgctga
     1681 accaacaggg cgagcgatac cgactcgacg gagtcgagat tcactgcaca cctgcgttcg
     1741 atcggatcat cgcctggttc gaggacgatt tcgcttggta gaccgcggac cgtcgtcgtg
     1801 cacggggcga tgagagctcg ccccagtcgt ggaatggcga catcgcgaga caacacaacg
     1861 actggtcgcc gaccgatctc cgggtgttca caccaccaga gatcaccacg tcgaggaaag
     1921 gcggtcatgt cgaagcgacg gcgttgcgga acgactcgag atcaccccag tcgtccggtt
     1981 caccgatggg gtggttctcg taggccttgg catattcggc gtcgatcgtc gcgcgtctct
     2041 cggctgcgag aagcgcggcg agcgcagcgt cgatcagctt cgagtcggtt gaatcgctac
     2101 gaaggcgccg agcgtcggtg aggaggtcgg cgtcaacggt ggtgctgatg cggattcggg
     2161 ccattcgcaa gtatggcacg actatgccac gactatgcca cggaagtgcc acggtgctcg
     2221 ctggcgatct cgtcggaggc gatggcgcag aatgggttgt tccgcaagcg caggagcact
     2281 gttgggctga taggcgctcg cggttcgggc gttcgagatg ttcacgcacg ttcgcggggg
     2341 tagcgagagg gttgccgtcg agattgaccg aaacgtcctc gtcggtagtt tccagtgcct
     2401 cgtcggtggg tggtccgtta ttagtggcca ctgacgattc gacaccgtca cccagacaac
     2461 ccaccaagtg accgaacctg aaccaccagc gcggaagccc cgtcacggca aggcgcggta
     2521 cgctcgcgct cttggcagat gtcgacgcgt accagtgaga cgcgctcgaa gccgatctca
     2581 ggggagtacc aatgtctgaa cgaaccgcgt ccgaccgagt ggcgtccgaa cgagtggcgt
     2641 ccgaacgagt gcatgcattc accgatgatg cgctcggtga tgacgacgcg gttgcgctcg
     2701 cggagcgggt tcgctccggt gaggtccatc ccagggagtt gctggaggcg gcgatcgcac
     2761 gggcggcaat ggtcgacccg acccttcgtg cggtcgaaac cccgatgtat gaccgacccc
     2821 gcaccggcgc cggttccgac gccccgtttt tcggcgtgcc gacctatctc aaggacaaca
     2881 ccgaagtcct cggcgtgccc accggaaacg gcagcgaggc ctacgtcgcc aagccggcac
     2941 agaaggacgc gccg
//
LOCUS       DAOOMA010000326         2949 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_40944_length_2949_cov_3.769523, whole genome shotgun sequence.
ACCESSION   DAOOMA010000326 DAOOMA010000000
VERSION     DAOOMA010000326.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 2949)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2949)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2949
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40944_length_2949_cov_3.769523"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..498)
                     /locus_tag="PLP95_09010"
     CDS             complement(<1..498)
                     /locus_tag="PLP95_09010"
                     /inference="COORDINATES: protein motif:HMM:NF021646.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB45978.1"
                     /translation="MKYVVCVPDGCSDEPTEELGGKTPLEVADMPVLAALAARSTVGR
                     AAVIPEGFPPGSDVGNMSILGYDPAIYHTGRAPIEAAALGLKLPADRLAYRTNLVTVS
                     SDGTMVDFAGGHPTNELASEIIAAIDAELGTGGDLGLEFHPGVQYRHIMVAPADWEVA
                     ECVPPH"
     gene            complement(548..1966)
                     /gene="thrC"
                     /locus_tag="PLP95_09015"
     CDS             complement(548..1966)
                     /gene="thrC"
                     /locus_tag="PLP95_09015"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012222531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HPB45979.1"
                     /translation="MRYVSTRGSAQPLEFAEVLLEGLATDGGLYVPEAWPALPDLSGD
                     ADYVETATAVMWPFVEGSISHDDFASIVADAYSTFEPPQGSTPDVVPVSDLGDNHFLA
                     EIYWGPTLAFKDIALQLVGRLFDHELERRHDKVTIVGATSGDTGSAAIEACRDREHID
                     IFILHPEGRTSEVQRRQMTTVPSDNVHNIAIEGTFDDCQDLVKALFADEEFRRRVKLS
                     AVNSINWARVMGQIVYYVTSARSVNRRLGRPLDAPVAFTVPTGNFGNIFAGYAAMKMG
                     LAISQLVVASNTNDILYRFFTTGRMDIEGVTPTLSPSMDIQVSSNLERLLFEMVGRDG
                     AAIAELMMAFRADNTASVAAEQLAWLSETFSAGRYTDDECLALIGALYRESGKLIDPH
                     TAVGVGVARSQRWDPEIPMITLGTAHPAKFPDAVEEATGVRPALPEHLADLFDRPERF
                     EVLAADAERLKHYVSSHLTAHG"
     gene            complement(2010..>2949)
                     /locus_tag="PLP95_09020"
     CDS             complement(2010..>2949)
                     /locus_tag="PLP95_09020"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020378380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HPB45980.1"
                     /translation="EVAEGAGVDLLFEAAVAGGIPLMRPLRESLVGERVTRVMGIVNG
                     TTNFILSQMSDHGTGYAEALAEAQSLGFAERDPTADVEGFDAGAKAAIIATVAFGCRV
                     VAGDVYHEGISNISAADIAYARQMGYVIKLLAICEQFDDGSVAVRVHPTMVPVDHPLA
                     GVAGSFNAVFVEGEAVGDLMFYGRGAGGAPTASAVLGDVIDAAQNLSQSTHGSLGTLS
                     QCRVRSIDELSSAFYLNLEVSDEPGVLAAVAGIFGEHDVSIRSMEQEGLGAQARLVFI
                     THEASESSVRSTLKALTALSAVQRVNSVLRVIGPDS"
BASE COUNT          527 a         1009 c          934 g          479 t
ORIGIN      
        1 gtgaggcggc acgcactcgg cgacctccca gtcggcggga gccaccatga tgtggcggta
       61 ctgcacgccc gggtggaact cgaggccgag gtcgccgccg gtgccgagtt cggcgtcgat
      121 cgcggcgatg atctccgagg ccaattcgtt ggtcggatgg ccgccggcga agtcgaccat
      181 cgtcccgtcc gaagacacgg tcaccagatt cgtacggtag gccagccgat ccgcaggaag
      241 cttcagtccg agcgccgcgg cctcgatcgg ggcacgcccc gtgtgataga tcgccgggtc
      301 gtaaccgaga atcgacatgt tgccgacatc ggatccgggc ggaaatccct cggggatgac
      361 ggccgcccgc ccgaccgtcg aacgcgctgc gagcgccgcg agtaccggca tgtccgcaac
      421 ctcgagcggc gtcttgcccc cgagttcttc ggtcggttca tcggaacacc catcgggaac
      481 gcagaccaca tatttcacgg caaccacggt actcggtgcc ggcaaacccc acgtcagtgg
      541 ccggaactca gccgtgggcg gtcagatgcg acgacacgta gtgcttgagt cgctcggcat
      601 cggccgcgag cacctcgaag cgctccggac ggtcaaacag gtcggcgagg tgctcgggta
      661 acgctggccg cacccctgtc gcttcctcga ccgcatcggg gaacttcgcc ggatgcgccg
      721 tgccgagcgt gatcatcgga atctccgggt cccaacgctg ggaccgggcg acgccgacgc
      781 ccaccgccgt gtgcggatcg atgagcttgc cggattcgcg atacagcgcg ccgatcagcg
      841 ccaggcactc gtcgtcggtg taacggcccg ccgagaacgt ctcgctcagc cacgccaact
      901 gctcggctgc gacactggcg gtgttgtcgg cccgaaacgc catcatcagt tccgcgatcg
      961 ccgctccgtc gcgcccgacc atctcgaaca gcaggcgttc gagattcgag ctcacctgga
     1021 tgtccatcga cgggctcagc gtcggcgtca ccccctcgat gtccatgcgc cccgtggtga
     1081 aaaaccggta caggatgtcg ttggtgttcg aagcgacgac gagttggctg atggccaacc
     1141 ccatcttcat cgcggcataa ccggcgaaga tgtttccgaa attgccggtg ggaacggtga
     1201 aggccaccgg ggcatcgagc ggacggccga gacgccgatt caccgaacga gccgaggtga
     1261 cgtagtagac gatctggccc atcacccgcg cccagttgat ggagttcacg gcgctcagct
     1321 tcacccgccg tcgaaactcc tcatcggcaa acagggcctt caccaggtcc tggcagtcgt
     1381 cgaaggtccc ctcgatggcg atgttgtgga cgttgtccga gggcaccgtg gtcatctggc
     1441 ggcgctgaac ctcggaggtc cgcccctcgg gatgcaggat gaagatgtcg atgtgttcgc
     1501 gatcccgaca ggcctcgatt gccgccgacc ccgtgtcgcc ggaggtcgcc cccacgatgg
     1561 tcaccttgtc gtgacgtcgt tccaattcgt ggtcgaacag tcggcccacg agttgcagcg
     1621 cgatgtcctt gaacgccagg gtcggccccc agtagatctc cgcgaggaag tggttgtcgc
     1681 cgagatcgct gaccggcacg acgtcgggcg tcgaaccctg cggcggttcg aaggtcgagt
     1741 acgcgtcggc gacgatggac gcgaagtcgt cgtggctgat cgacccctcg acgaagggcc
     1801 acatcaccgc ggtcgcggtc tccacgtagt cggcatcgcc actcagatcc ggcaaggccg
     1861 gccaggcttc gggcacatac aggccaccgt cggtggcgag cccttcgagc agaacctccg
     1921 cgaattccaa cggctgtgcc gatccacggg tactcacata acgcatgtcg ctgctgctcc
     1981 tcgctgcttc gatccccgag atgtgcgggt caactgtcgg gtccgatgac ccgcaacacg
     2041 gaattgaccc gctgcaccgc gctcaacgcg gtgagggctt tgagggtgga acgcaccgac
     2101 gactccgacg cctcgtgggt gatgaacacc aaccgagcct gcgcgccgag cccctcttgt
     2161 tccatcgaac ggatcgacac gtcgtgctcg ccgaaaattc cggcgaccgc agcgagcact
     2221 ccgggctcgt cgctcacctc gaggttgagg tagaaggcag aggacaactc atcgatcgaa
     2281 cgcacacggc actgcgacaa cgtgccgagc gatccgtgcg tggactgcga gaggttctgg
     2341 gcggcgtcga tcacgtcgcc gagcacggcc gacgcggtcg gggccccacc ggcaccgcgc
     2401 ccgtagaaca tcaggtcgcc caccgcctca ccctcgacga acacggcgtt gaaactcccg
     2461 gccacgcctg cgagcgggtg atcgaccggc accatcgtgg ggtgaacgcg cacggcgacc
     2521 gaaccgtcgt cgaattgttc gcagatcgcc aacagcttga tcacataccc catctggcgg
     2581 gcataggcga tgtcggccgc gctgatgttc gagatgccct cgtggtacac gtcgcccgcg
     2641 acgacccgac acccgaaggc gaccgtcgcg atgatcgccg ctttcgcccc ggcatcgaag
     2701 ccctcgacat cggcggtcgg gtcgcgttcc gcgaacccca ggctctgcgc ctccgcgagc
     2761 gcctctgcat acccggtgcc gtggtcgctc atctggctga gaatgaaatt cgtcgtgccg
     2821 ttgacgatgc ccatcacccg ggtgacgcgc tcgccgacca acgactcccg caacgggcgc
     2881 atcagcggaa tcccaccggc gacggcggcc tcgaacaaca ggtcgacccc ggcaccttcg
     2941 gccacctcg
//
LOCUS       DAOOMA010000327         2940 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_41118_length_2940_cov_3.084575, whole genome shotgun sequence.
ACCESSION   DAOOMA010000327 DAOOMA010000000
VERSION     DAOOMA010000327.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 2940)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2940)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2940
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41118_length_2940_cov_3.084575"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1180)
                     /locus_tag="PLP95_09025"
     CDS             complement(<1..1180)
                     /locus_tag="PLP95_09025"
                     /inference="COORDINATES: protein motif:HMM:NF013362.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HPB45981.1"
                     /translation="MSESRKPAPRPGQRPGQKPGHKSGPKPGHKSGPKPGPKSAREIA
                     IDALVAIDNDQSFANLRLGPILERSGLDDRDRRFVTEMVYGAIRRRRSLDHLVDRFLS
                     SDPPSVARAALRLGAYQLHYLEVPDHAAVSATVDATPKRFRGVVNAVLRKVASNPVVW
                     PDVGTELSYPDWIVERLSADLGSDVAIEALEVMNTAPEVAIRSDGYIQDRSSQRVVAA
                     LPIEAGQLVLDACAAPGGKATAMAGRGARVIAADLRLKRVGLIAKNSAATAANRPDGG
                     ALWPVVADGRHLPLARRSVDGVLVDAPCSGLGVLRRRADARWRIEPADVDRLASIQFD
                     LLVEAARAVSGGGWLAYSVCTLTNAETIDVATRFVAQHPDFEPIEFVEGPWEAIGTGG
                     C"
     gene            complement(1177..2055)
                     /locus_tag="PLP95_09030"
     CDS             complement(1177..2055)
                     /locus_tag="PLP95_09030"
                     /inference="COORDINATES: protein motif:HMM:NF012760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HPB45982.1"
                     /translation="MKLVYLGTPAIAVPPLRALVAAGHEVDLVVTGADTRRGRGKQTS
                     PNPVKAAALELGLRVEHDPDAVLDSDAQLGVVVAYGAILRPHLLAHLPFVNLHFSLLP
                     RWRGAAPVERAILAGDAETGVCVMAIEEGLDTGGVFARESTPIGDKTLMDLWDELSDT
                     GSRLLVDWLPDAFVEGRLVEPTPQEGEATYAPKLTAQDRQVDLASPAVEQFRRIRLGN
                     AWTVVGDKRLKLLAATLVGDGDGDGDGDGAGAGAGAGAGDVTIRYETVQAEGKTPMAF
                     EDWWRGATAEQQAAFS"
     gene            complement(2068..2661)
                     /gene="def"
                     /locus_tag="PLP95_09035"
     CDS             complement(2068..2661)
                     /gene="def"
                     /locus_tag="PLP95_09035"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012229394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HPB45983.1"
                     /translation="MARSATPYRIRIIGDPVLREATPDVTDIDGALVQLVEDMFEVLY
                     TTDTGVGLAAPQVGVGQRIFVYDIGDDDEPGVLINPRITESDGEWTYSEGCLSIPGQY
                     FEIVRPKQIEVSALDLDGNEITFEADEFWARLIQHELDHLDGVLMIDHLDEDQRTAAK
                     KAVRELQMQAADRSDLGRQGVGLASEAPAKKRFGLFS"
     gene            2756..>2940
                     /locus_tag="PLP95_09040"
     CDS             2756..>2940
                     /locus_tag="PLP95_09040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB45984.1"
                     /translation="MTSGDSEQHHYFDPSPTVPSQPREVRLDLVDLSLRLATDRGVFS
                     ADRVDPGTRVLLGEAPPP"
BASE COUNT          431 a         1013 c          998 g          498 t
ORIGIN      
        1 ggcaccctcc cgttccgatg gcctcccacg ggccctcgac gaactcgatg ggttcgaaat
       61 ccgggtgctg agcgacgaat cgcgttgcga cgtcgatcgt ctccgcgttg gtcagggtgc
      121 acacgctgta ggcgagccat ccgccgccgc tcacggcgcg ggcggcctcg accagcagat
      181 cgaactggat cgacgccagg cgatcgacat ctgcgggctc gatgcgccat cgtgcgtcgg
      241 cccgccggcg aagcaccccg agccccgaac acggtgcgtc gacgagcacc ccgtcgacgc
      301 tgcgccgtgc cagcggtagg tggcgcccgt cggccaccac cggccacagc gcgccaccgt
      361 ctggccggtt cgcggccgtc gctgccgagt tcttggcgat caggccgacc cgcttgagcc
      421 gcagatcggc ggcgatcacc cgagcaccgc gaccggccat ggcggtggcc ttgccgccgg
      481 gcgctgcgca cgcgtcgagc accaactgcc cggcttcgat cggcaacgcg gcgacgacgc
      541 gttggctgct gcgatcctgg atgtagccgt cgctgcgaat cgccacctca ggggcggtat
      601 tcatcacctc gagcgcctcg atcgctacgt cggagcccag atccgcgctg agccgctcga
      661 cgatccagtc cgggtagctc aactcggttc cgacatcggg ccacacgacc gggttcgagg
      721 ccaccttgcg caacacggcg ttcacgacgc cgcgaaaccg cttcggagtc gcgtccacag
      781 tggcggacac cgctgcgtgg tcgggcacct cgaggtagtg cagttggtag gcgccgaggc
      841 gcaacgcggc gcgggccacc gatggagggt cgctggacag gaaccgatcg acgagatgat
      901 cgagggaacg gcgacgacga atcgctccat agaccatctc ggtgacgaac cgtcgatcgc
      961 ggtcgtcgag tccgctgcgc tccagtatcg ggccgagtcg aagattggcg aaggattggt
     1021 cgttgtcgat ggcgacgagt gcgtcgatgg cgatctcacg ggccgacttc gggccgggct
     1081 tcgggccgga cttgtggccg ggcttcgggc cggacttgtg gccaggtttc tggccgggtc
     1141 tctggccggg tctcggggcg ggcttcctcg actcactcat gaaaacgccg cttgctgttc
     1201 tgcggtggcg ccgcgccacc agtcctcgaa ggccatgggg gtctttccct ccgcctgaac
     1261 cgtctcgtag cggatcgtca cgtcaccggc accggcaccg gcaccggcac cggcgccgtc
     1321 gccgtcgccg tcaccgtcgc cgtcgccgac gagtgtcgcc gccagcagtt tcaggcgttt
     1381 gtcgccgacg acggtccagg cgttgccgag gcggatccgg cgaaactgct cgacggccgg
     1441 cgacgccagg tcgacctggc ggtcctgcgc ggtgagtttc ggtgcgtacg tcgcctcccc
     1501 ctcctgtggg gtcggttcga ccaggcgacc ctcgacgaac gcgtccggca accagtcgac
     1561 gagcagacgc gaccccgtgt cgctcagttc gtcccacagg tccatcagcg tcttgtctcc
     1621 gatcggggtc gactcacgtg cgaacacccc gccggtgtcg aggccctcct cgatggccat
     1681 gacgcagacc ccggtctcgg catctcctgc gaggatcgcc cgctcgaccg gggcagcccc
     1741 gcgccatctc ggcagcagcg aaaagtgcag gttcacgaac gggagatgtg cgagcaggtg
     1801 cgggcgcaga atcgctccat aggcgaccac gacgccgagt tgtgcgtcgc tgtcgagcac
     1861 cgcatcgggg tcgtgttcca cccgcagacc cagttcgagt gcggccgcct tcaccgggtt
     1921 cggcgaggtc tgcttgcccc ggcctcggcg ggtgtcggcg ccggtcacga ccaggtcgac
     1981 ctcgtggccc gcggcgacga gtgcgcgcag cggcggcacc gcgatggctg gggttccgag
     2041 gtagacgagt ttcatggcgc tccggcgtta cgagaacaac ccgaagcgct tcttggccgg
     2101 cgcttcagag gccaaaccga ccccctgccg gcccagatcg gaacgatcgg ccgcctgcat
     2161 ctgcagttca cgcaccgcct ttttggccgc cgtgcgttgg tcctcgtcga ggtgatcgat
     2221 catcagcacc ccgtcgaggt ggtcgagttc gtgttggatc aggcgcgccc agaactcgtc
     2281 ggcctcgaag gtgatctcgt tgccgtcgag gtcgagtgcg ctcacctcga tctgcttggg
     2341 gcggacgatc tcgaagtact gcccggggat cgacaggcac ccctcggagt aggtccactc
     2401 gccgtccgat tcggtgattc gagggttgat gagcacaccc ggttcgtcgt cgtcgccgat
     2461 gtcgtagacg aagatgcgct gcccgacgcc gacctgtggg gccgcaaggc cgacgccggt
     2521 gtcggtggtg tagagcacct cgaacatgtc ctcgacgagt tgcacgaggg ccccgtcgat
     2581 gtcggtgacg tccggggtgg cttcgcgcag caccggatcg ccgatgattc ggattcggta
     2641 cggggttgca cttcgggcca tggctcaagt ctagggcacc acccggagcc gccatctgcg
     2701 aggtccacag ctgaggcttg cgtcgctgcg gcccaggtgc cgctacgttg gccgggtgac
     2761 cagcggggat tccgaacagc accattattt cgacccctcg ccgacggttc cgtcgcagcc
     2821 ccgcgaggtg cgcctcgatc tcgtcgatct gtcccttcgg ctcgccaccg atcggggggt
     2881 gttctcggcc gatcgcgtcg accccggaac ccgcgtgctg ctgggcgagg ccccgccgcc
//
LOCUS       DAOOMA010000328         2940 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_41119_length_2940_cov_3.072097, whole genome shotgun sequence.
ACCESSION   DAOOMA010000328 DAOOMA010000000
VERSION     DAOOMA010000328.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 2940)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2940)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2940
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41119_length_2940_cov_3.072097"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..803
                     /locus_tag="PLP95_09045"
     CDS             <1..803
                     /locus_tag="PLP95_09045"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HPB45985.1"
                     /translation="LPDAHTQAPTQGSVILAAVLLKLGTYGFVRIAINILPDAAKSWA
                     PVIGILAAIGIIYGALGCLAQTDMKRLIAFSSVAHMGYVMLGISTLTKHGINAAMFGM
                     VAHGLITGMLFFVAGSIKERYHTLDMARLGGLLKSAPKMGWILGFASMASLGLPGLAG
                     FWGEFPAILATFNPAEGLSLPLFRTLMVVAAIGTTFAAAYLLWMYQRVAFGVPTEEFE
                     NDPHITDVNITEWIAWTPFLAAIVVFGFFPGLMFDVTETALKALNLGA"
     gene            807..2306
                     /locus_tag="PLP95_09050"
     CDS             807..2306
                     /locus_tag="PLP95_09050"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit N"
                     /protein_id="HPB45986.1"
                     /translation="MLAFTAPVLDWHAFAPEIVLVATLCVMLIVDVIQLDRARAVIGT
                     IAGLGFLGALVPIITLASSDVAVRSMFGGAYVVDQTSLLLKGLFVVSAYVVVLLSTNY
                     VADGDYWESEYYHTLVASVIGMTVITSARDMISIFVAFELLSMPTYMLASWRKRDTRS
                     NEAGLKYFLMGVFASGLMLYGMSLLYGWGGSTLLSDLAVAFSKDSSPAVLMGSLMVIV
                     GFGFKVSAVPFHQWAPDTYEGAPTPVTAFLAVASKTAGVVGLIQFCALLFGPVQGDLT
                     KATTPMFLILSIASMLVGNMIALKQTNLVRMLAYSGIGQAGYILAPFAVIGSDKAADG
                     VVPAVVTYLVIYAAMNLGAFGVVIAVARKTRSAEISSYAGLFHYAPGLTVVMTAFLFS
                     LIGFPPAAGWYGKFAIFKVLIDADTPAGYTLAIAIVVTSTIAAYYYLNVAKTMWFDDV
                     ADGDTTPIKVVPAVGVALAIAVVITMVLGVFPSLISDAANFTPMAAAGI"
     gene            2344..>2940
                     /locus_tag="PLP95_09055"
     CDS             2344..>2940
                     /locus_tag="PLP95_09055"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014673.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HPB45987.1"
                     /translation="MSGPEVSGSPGAGSAGAGPELSGPERFDRYVERCLYDPISGFYA
                     AHGQAGGSRGDFLTSPEVGPLFGHLIGRYLDTVWRDLGEPAPFTVVECGAGRGTLARA
                     VLSSAPECLAALDYVCVERSERLRADARDLLGESARVCGSLEEVEAPFVGVLMANELL
                     DNLAFRLIVRDGGGWAEVFVVDGAPVLGEFDGSGGPVNV"
BASE COUNT          407 a          991 c          939 g          603 t
ORIGIN      
        1 ggctgcccga tgcccacacc caggcgccca cccagggttc ggtcatcctg gccgccgtgc
       61 tgctgaagct cggcacctac ggcttcgtgc gcatcgccat caacattctc cccgacgccg
      121 ccaagagctg ggctccggtc atcggcatcc tcgccgccat cggcatcatc tacggtgcgc
      181 tcggctgcct ggcccagacc gacatgaagc ggctcatcgc cttctcgtcg gtggcccaca
      241 tgggctatgt gatgctcggc atctcgacgc tcaccaagca cggcatcaac gccgcaatgt
      301 tcggcatggt ggcgcacggc ctcatcaccg gcatgttgtt cttcgtcgcc ggatccatca
      361 aggagcgcta ccacaccctc gacatggccc gcctcggtgg cctgctgaag tccgccccga
      421 agatgggctg gattctcggc ttcgcctcga tggcctcgct tggccttccc ggcctcgccg
      481 gtttctgggg tgagttcccc gccatcctcg cgacgttcaa ccccgccgag ggcctgagcc
      541 tgccgttgtt ccgcacgctc atggtcgtcg ccgccatcgg caccaccttc gccgcggcgt
      601 acctgttgtg gatgtatcag cgagtcgcct tcggtgtgcc caccgaggaa ttcgaaaacg
      661 acccgcacat caccgacgtc aatatcaccg aatggatcgc atggaccccg ttcctcgccg
      721 cgatcgtcgt gttcggtttc ttccccggcc tcatgttcga cgtgaccgag accgccctga
      781 aagctctgaa cctgggagcc tgatcgatgc tcgcctttac cgcaccggta ctcgactggc
      841 acgcattcgc gccggagatc gtgctggtcg cgaccctctg tgtgatgttg atcgtggacg
      901 tgatccagtt ggaccgcgcc cgcgccgtca tcggtacgat cgccggcctc ggtttcctgg
      961 gcgccttggt gccgatcatc accctcgcca gctccgacgt ggccgtccgg tcgatgttcg
     1021 gaggggccta cgtcgtcgac cagacctcgc tgttgctcaa agggctcttc gtcgtgtcgg
     1081 cctacgtcgt ggtgttgttg tccaccaact acgtggccga cggcgactac tgggaatcgg
     1141 agtactacca caccctcgtc gcctcggtga tcggcatgac cgtcatcacc tcggcccgag
     1201 acatgatctc gatcttcgtg gccttcgagc tgttgtcgat gccgacctac atgctggcga
     1261 gctggcgcaa acgcgacacc cgctccaacg aagccggcct caagtacttc ctgatgggcg
     1321 tgttcgcctc gggtctcatg ttgtacggca tgtccttgct gtacggctgg ggcggctcga
     1381 cgctgctcag cgacctggcc gtggccttct cgaaggacag ctcgccggcc gtgctcatgg
     1441 gttcgttgat ggtgatcgtc ggcttcgggt tcaaggtgtc ggccgtgccg ttccaccagt
     1501 gggcccccga cacctacgaa ggtgcgccga ccccggtcac cgccttcctg gccgtcgcct
     1561 cgaagaccgc cggtgtggtc gggctcatcc agttctgcgc cctgctgttc ggcccggtgc
     1621 agggcgacct caccaaggcg accacgccga tgttcctgat cctgtcgatc gcctcgatgc
     1681 tcgtcggcaa catgatcgcc ctcaaacaga cgaacctcgt gcgcatgttg gcctactccg
     1741 gtatcggcca ggccggctac atcctcgccc cgttcgccgt gatcggttcc gacaaggcgg
     1801 ccgatggagt ggtgcccgcc gtggtgacct acctggtgat ctacgcggcg atgaacctcg
     1861 gagcgttcgg cgtcgtcatc gcggtggccc gcaagacccg ctcggcggaa atctccagct
     1921 acgccgggct cttccactac gcacccggcc tcacggtggt gatgaccgcg ttcttgttct
     1981 cgctcatcgg gttccccccg gctgcgggct ggtacggcaa gttcgccatc ttcaaggtgc
     2041 tcatcgacgc cgacaccccc gccggataca ccctggcgat cgccatcgtg gtcacgtcca
     2101 caatcgctgc gtactactac ctcaacgtcg cgaagacgat gtggttcgac gacgtggccg
     2161 acggcgacac caccccgatc aaggtcgtcc ccgcagtggg tgtggccctg gcgatcgcgg
     2221 tggtcatcac catggtgctg ggtgtgttcc cgtcgctcat ctccgacgcc gcaaacttca
     2281 cgccgatggc ggccgccgga atctgagcgg gcgccggaat ctgaccgggc atcaggtcct
     2341 gagatgtcgg gccctgaggt gtcgggttct cctggcgctg gttcggccgg cgccggtcct
     2401 gagctgtctg gtcccgagcg tttcgaccgg tacgtcgagc gttgcctcta tgacccgatt
     2461 tcgggtttct acgcggcgca cggccaggcc gggggatcgc gtggcgactt cctgaccagc
     2521 cccgaggtcg ggccgttgtt cgggcatctg atcgggcggt acctcgacac cgtgtggcgc
     2581 gacctcggcg aacccgcgcc gttcaccgtc gtcgaatgcg gggccggtag gggaacgttg
     2641 gccagggcgg tgttgtcgag tgcgcccgag tgcttggcgg cgttggacta cgtgtgtgtg
     2701 gagcgctcgg agcggctgcg ggctgacgct cgtgatctgc tgggcgagtc ggcgcgggtg
     2761 tgtgggtcgc tcgaagaggt ggaggcgccg ttcgtcgggg tgctgatggc gaacgaactg
     2821 ctcgacaacc tggcgtttcg cttgatcgtg cgcgacggcg ggggctgggc cgaggtgttc
     2881 gtcgtggacg gggcgccggt gctcggcgag ttcgatggct cgggtgggcc ggttaacgtg
//
LOCUS       DAOOMA010000329         2935 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_41218_length_2935_cov_4.862847, whole genome shotgun sequence.
ACCESSION   DAOOMA010000329 DAOOMA010000000
VERSION     DAOOMA010000329.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 2935)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2935)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2935
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41218_length_2935_cov_4.862847"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(69..326)
                     /locus_tag="PLP95_09060"
     CDS             complement(69..326)
                     /locus_tag="PLP95_09060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012227562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HPB45988.1"
                     /translation="MSGTEVSERAEDVVRHVVTAIVDHPDVVRVSTDTDRDPARINIE
                     VGEGDMGRVIGRRGRVANAIRALGRAAAAIDGEDVDVEFVD"
     gene            complement(323..592)
                     /gene="rpsP"
                     /locus_tag="PLP95_09065"
     CDS             complement(323..592)
                     /gene="rpsP"
                     /locus_tag="PLP95_09065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015798903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HPB45989.1"
                     /translation="MAVRLRLMRMGKKKQPTYRIVAADARSPRDGRFIEIVGTYDPRR
                     EPSAIALDNEKAVKWLRNGAQPSERVEKLLKISGAWDEFQASKSK"
     gene            complement(797..2281)
                     /gene="ffh"
                     /locus_tag="PLP95_09070"
     CDS             complement(797..2281)
                     /gene="ffh"
                     /locus_tag="PLP95_09070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012227559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HPB45990.1"
                     /translation="MFEALSDRFEGIFKRLRNSGHLSADQVEEVLREIRVALLEADVN
                     IKVVRSLQNRIRERAVGTEVHEALTSAQQIIKIVNEELINTLGGETMKITFSSKPPTV
                     VLMAGLQGSGKTTNSAKLARWFKAQGRNPILVGADLQRPAAVEQLRVLAQQIDVPVYS
                     EPTDPVAVAQGAIAEARRLGRDVVIVDTAGRLAIDEELMAQVREISDAVDPDYTFLVI
                     DAMLGQDAVTVAEHFNETLELDGVILTKLDGDARGGAALSVKEVVGKPIAFASTGEKL
                     DDFDSFHPDRLASRILGMGDMLTLIEKAEEVFDQEQSEIAAERLMAGTFTFDDFVEQL
                     NALRKMGSLSSIIGMLPGIPKEVRDIEIGDKEVGRVEAIIRSMTIEERANPDVIDTSR
                     RQRIARGSGTNPTEVQQLITQFKEMRRMMQQMGNMDRFSKRKAKKMKKGAKRKGKGGN
                     PSRVSAKGTMPLGQGMRMPGVDPLAAELEKAGIDLPGLDFGRDS"
     gene            complement(2353..>2935)
                     /gene="ftsY"
                     /locus_tag="PLP95_09075"
     CDS             complement(2353..>2935)
                     /gene="ftsY"
                     /locus_tag="PLP95_09075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020378137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HPB45991.1"
                     /translation="GVGKTTTIGKVGKRLTDEGRSVVMAAGDTFRAAAAEQLDTWAQR
                     CGADIVRGAEGSDPSSVIFDGVAKAAAQGSEFVLADTAGRLHTKSNLMDELGKVRRVA
                     DKGAGSVTEVLLVLDATTGQNGLIQAQQFTDAVDLTGVVLTKLDGSAKGGIVFAIQSQ
                     LGIPVKLVGLGESAQDLVDFDADQFVDALFAED"
BASE COUNT          441 a          959 c          983 g          552 t
ORIGIN      
        1 caccccgcag cccgtgggcc ttgccgatcc ggccgacctc cagcagtgcc atgggatgtc
       61 gtcgttgctc agtcgacgaa ttcgacgtcg acgtcctcgc cgtcgatcgc cgcagcggca
      121 cggcccagcg cgcgaatggc gttcgcgacg cggccgcggc ggccgatgac ccggcccatg
      181 tcgccctcgc ccacttcgat gttgattcgg gcgggatcgc ggtcagtgtc ggtggagacc
      241 cgcacgacgt cgggatgatc gacgatcgcg gtcaccacgt gacgcacgac gtcttcagcg
      301 cgttcgctca cctcggtgcc gctcatttcg acttggaggc ctggaactcg tcccaggcgc
      361 ccgagatctt cagaagcttc tcgacgcgct cgctcggctg ggcgccgttg cgaagccact
      421 tgacagcctt ttcgttgtcg agggcgatgg cggagggctc acggcgcgga tcgtaggtgc
      481 cgacgatctc gatgaagcgc ccgtcgcgtg gcgagcgggc atcggcggcg acgattcggt
      541 aggtgggctg cttctttttg cccatgcgca tgaggcggag acggactgcc acagtttctc
      601 tcttatcttc agtcgaggtg ccgcggggaa tgcgaacaag cgcggcgatg gaacattgtg
      661 atcggtgatc ggggtaccgg gcaactcccg ctgcccggac acggggaacg atcctaggcc
      721 atggcggcac ccgagtgggc cacccgaggc cgaaaagacc gggtcggggt acccctccgg
      781 tcgtcgggac gcagactcag gagtcgcgcc cgaagtcgag acccggaagg tcgatgccgg
      841 ccttttcgag ttctgccgcg agcgggtcga cccccggcat ccgcatcccc tgaccgagcg
      901 gcatcgtgcc cttcgcactc acgcgactcg ggttgccgcc cttacccttg cgctttgccc
      961 ccttcttcat cttcttggcc ttgcgcttcg agaagcggtc catgttgccc atctgctgca
     1021 tcatgcgccg catctctttg aactgcgtga tgagctgttg gacctcggtc gggttcgtgc
     1081 cggagcccct cgcgatgcgc tggcggcgac tcgtatcgat cacgtcgggg ttggcgcgtt
     1141 cctcgatggt catcgaccgg atgatcgcct cgacgcggcc gacctcctta tcgccgatct
     1201 cgatgtcgcg gacctctttg ggaatgcccg gcaacatgcc gatgatcgac gacagcgacc
     1261 ccatcttgcg cagcgcgttg agctgttcga cgaagtcgtc gaaggtgaac gtgccggcca
     1321 tgaggcgttc ggcggcgatc tcggactgtt cctgatcgaa gacctcctcg gccttttcga
     1381 tgagggtgag catgtcgccc atgccgagga tgcggctggc gagtcgatcg gggtggaagc
     1441 tgtcgaagtc gtcgagcttc tcaccggtcg acgcgaaggc gatcggcttg ccgacgacct
     1501 ccttcaccga cagcgcggcg ccgccgcggg catcgccgtc gagcttggtg aggatcacgc
     1561 catcgagttc gagcgtctcg ttgaaatgct cggcgacggt gaccgcgtcc tggcccaaca
     1621 tggcgtcgat gaccaggaag gtgtagtcgg ggtcgaccgc gtcggaaatc tcgcggacct
     1681 gggccatcaa ctcctcgtcg atggcgagac gaccggcggt gtcgacgatg accacgtcgc
     1741 ggcccaggcg gcgcgcctcg gcgatggccc cctgggcgac ggcgaccgga tcggtcggct
     1801 cggagtagac cggcacgtcg atctgttggg cgagcacccg gagctgttcc accgcggcag
     1861 gccgttgcag gtcggcgccg acgagaatcg ggttgcgacc ctgggccttg aaccagcggg
     1921 cgagcttcgc cgagttcgtc gtcttgcccg aaccctgcag gccggccatc agcaccacgg
     1981 tgggcggctt cgacgagaac gtgatcttca tcgtctcgcc gccgagggta ttgatgagtt
     2041 cctcgttgac gatcttgatg atctgctggg ccgaggtcag cgcctcgtgg acctcggtgc
     2101 cgaccgcgcg ttcacggatc cggttttgga ggctgcggac gaccttgatg ttgacgtccg
     2161 cctcgaggag ggcgacacga atctcgcgca ggacctcttc cacctgatcg gcgctgaggt
     2221 gtccactgtt gcggagacgc ttgaagatcc cctcgaatcg atctgacagt gcttcaaaca
     2281 tggcgtggtc agcctacgtg actcggtgcg aggtccggtg actccgtgcg gggtccaccg
     2341 acccggtgcg ggtcagtcct ccgcgaacag ggcgtcgacg aactgatccg cgtcgaagtc
     2401 gacgaggtcc tgggcgcttt cgccgagtcc gacgagtttg accgggattc ccagctggct
     2461 ctgaatggcg aacacgatgc cgcccttcgc cgagccgtcg agcttggtga gcaccactcc
     2521 ggtgaggtcg accgcgtcgg tgaactgctg agcctggatg agcccgttct ggccggtggt
     2581 cgcgtcgagc accaacagca cctcggtcac cgacccggcg cccttgtcgg ccactcgacg
     2641 caccttgccg agctcgtcca tcaggttcga cttggtgtgg agacgaccgg cggtgtccgc
     2701 gagcacgaac tcgctgccct gcgcggcggc cttggccacg ccgtcgaaga tgaccgagct
     2761 cggatccgac ccttcggcac cgcgcacgat atcggcgcca cagcgctgcg cccacgtatc
     2821 gagctgctcg gctgcggcgg cgcggaaggt gtcgccggcg gccatgacga cgctgcggcc
     2881 ctcgtcggtg agtcgcttgc cgaccttgcc gatcgtggtg gtcttgccga ccccg
//
LOCUS       DAOOMA010000330         2935 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_41224_length_2935_cov_3.915625, whole genome shotgun sequence.
ACCESSION   DAOOMA010000330 DAOOMA010000000
VERSION     DAOOMA010000330.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 2935)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2935)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2935
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41224_length_2935_cov_3.915625"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..652)
                     /locus_tag="PLP95_09080"
     CDS             complement(<1..652)
                     /locus_tag="PLP95_09080"
                     /inference="COORDINATES: protein motif:HMM:NF012797.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HPB45992.1"
                     /translation="MADESGALSASNSRIKQLRRLSGRRSARHADGEFVIEGPVLVSE
                     ALEAGVAVRTVFVDAAHDGLRERFRHLGATHGVETLEVASGVLGSVLSAVSPQPVCAI
                     AETTLDRPLEDVAQMAADRSRALLVLVDVNDPGNAGTLLRAAESSGAAGLVLLGNSVD
                     LYNPKVVRGSAGSMFRLAIAVGSDMSLDEALDALRRRGIASVAAVARGGVSHLEAPL"
     gene            complement(682..1068)
                     /gene="rplT"
                     /locus_tag="PLP95_09085"
     CDS             complement(682..1068)
                     /gene="rplT"
                     /locus_tag="PLP95_09085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004192938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HPB45993.1"
                     /translation="MARVKGGVHAKKKHKNVLKKAKGYYGNRSRTFRVANQSVMHAGQ
                     YAFRDRRARKGEMRKLWIQRINAACRLNDISYSRFMGGLKAADIEIDRKSLADIAVVD
                     PAAFSALVEAAKAANDAKASNAANAA"
     gene            complement(1110..1304)
                     /gene="rpmI"
                     /locus_tag="PLP95_09090"
     CDS             complement(1110..1304)
                     /gene="rpmI"
                     /locus_tag="PLP95_09090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007840008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HPB45994.1"
                     /translation="MPKMKTHRGAKKRFKVTGSGKIMRRKVNRSHILEKKPSKRTRRL
                     AGEAELPKGDAQRVRRQLGI"
     gene            complement(1444..2091)
                     /gene="infC"
                     /locus_tag="PLP95_09095"
                     /pseudo
     CDS             complement(1444..2091)
                     /gene="infC"
                     /locus_tag="PLP95_09095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012515489.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
     gene            2313..2855
                     /locus_tag="PLP95_09100"
     CDS             2313..2855
                     /locus_tag="PLP95_09100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB45995.1"
                     /translation="MSLWTPDGERPVPPKQSTAQPSSSPTAAQAASQSASVRGQGGAA
                     IEVTPELAEALATAGIDVATLSPEQIAEATQMLAEMGRVRQEMLAVPASEIVANHLMG
                     IYELGAIHLGENPPNFAEATIAIEALRAVLDRLSGKFADAEPVLRQALTQLQQVFVEL
                     KEQVEARPGSPADESDDPDA"
BASE COUNT          472 a          988 c          930 g          545 t
ORIGIN      
        1 ccagcggagc ctcgaggtga ctgactcccc cgcgcgccac cgctgctacc gacgcgatac
       61 cacggcgacg cagcgcgtcg agcgcctcgt caagactcat gtccgacccg accgcgatgg
      121 cgagccgaaa catcgacccc gcggaaccac gcacgacctt cgggttgtag aggtcgacac
      181 tgtttcccaa caacaccaac ccggcggccc cggaactctc ggcggcgcgc aggagcgtgc
      241 cggcgtttcc cgggtcgttc acgtcgacca gcaccagtaa cgctcggctg cggtcggccg
      301 ccatctgcgc cacgtcctcg agcggccgat ccagggtcgt ctcggcgatc gcacacaccg
      361 gctgcgggga cacggcgctc agcaccgaac ccagcacgcc gctcgcgacc tcaagcgtct
      421 cgacgccgtg ggtcgcaccc aggtgtcgga accgctcgcg tagcccgtcg tgtgcggcgt
      481 cgacgaagac ggttcgcacg gcaacgccgg cctcgagcgc ttcgctcacc agcaccggcc
      541 cctcgatgac gaattcaccg tcggcgtgac gcgcactgcg gcgccccgag aggcgccgca
      601 gttgcttgat tctcgaattt gaggcgctca gcgcccccga ttcgtcagcc atcgattggg
      661 accgacctcg acggtgcccg attacgcggc gttcgcggcg ttcgacgcct tggcatcgtt
      721 ggccgccttg gcggcttcga cgagggccga gaacgcggcc gggtcgacca cggcgatgtc
      781 ggcgagcgac ttgcggtcga tctcgatatc ggcggccttg aggccaccca tgaaccggct
      841 gtagctgatg tcgttgagac ggcaggccgc gttgatgcgc tggatccaca gcttgcgcat
      901 ctcgcccttg cgggcacggc ggtcgcggaa cgcatactgc cccgcatgca tcaccgactg
      961 gttggcgacg cggaacgtgc gagaccggtt gccgtagtaa cccttcgcct tcttcagtac
     1021 gttcttgtgc ttcttcttgg cgtgaacgcc acccttgaca cgtgccattg ggaactcctc
     1081 gttagatctg gtggggtgtc agtaggcgat cagatgccga gctgacgacg gacacgctga
     1141 gcatcgccct tgggaagctc ggcctcaccg gcaaggcgac gggtgcgctt tgacggcttc
     1201 ttttcgagga tgtgggaccg gttgaccttg cggcgcatga tcttgccgga accagtcacc
     1261 ttgaagcgct tcttggcgcc gcggtgggtc ttcatcttgg gcatgtcgtc acgattcctt
     1321 cgttgcttcc tccgacccgg cgccgtcggc gtcgagcggg atttctgttt cggcggcatc
     1381 atccgccgta gcggcggcct ctgctgcctc ggcggccgca cgcttctgag cggcggcctg
     1441 agccttcttg tcgggaccga gcaccatggt catgttgcga ccatcctgct tcggaaacac
     1501 ttcgacccga ccaacgtctg caaccttctc ggcaacctga tcgaggatct tgcgaccgag
     1561 ttcggggtgt tgcatctcac gaccacggaa catgatcgtg atcttcacct tgtgtccctc
     1621 gccgaggaac tgctccacct tgcgggtctt ggtgtcgaag tcgccgccgc cgatcttggg
     1681 gcggtacttc atttccttga ccgtgatgtt ggtggccttc ttgcgggatt ccttggctcg
     1741 ttgctgggcc tcgaacttgt acttgccgta atccatgatc cggcacaccg gaggattggc
     1801 gtttccggcg acctcgacga ggtcgagttc ggccgctcgg gcgatggaca gggcctcagg
     1861 cagggtcttc atgcccagct gcgctccgtc ctgaccgatg agtcgcacct cgcgggcgcg
     1921 aattcggtca ttgatccgag gttcatcatt gcgtggtggt ggtgctatga cccgacttct
     1981 tccactcaca cgtcctttcg acgttggttc gtttgggtgg cgaccccatc cccggtcgga
     2041 gttcgagcgg cgcccgaaaa cgatgacagc ggatgtcgga atcgtcgaca tccgctgcga
     2101 acatcctggt cgggcgacga cgtgtcgcac ggtgagatgg tctcaagcgt cgacgctggc
     2161 gcactcggtg ccggggatac ctcatcgagg tggaccccac ggggaaaccc cgctgcggtg
     2221 gtggccaggt ggggcatgcc cggaggcggc ccactttccg ttgagttgcg ggaactacac
     2281 tagcactccc gaccagacaa aggacagttc acgtgagcct gtggaccccg gacggcgaac
     2341 gccccgtccc cccgaagcaa tcgaccgccc agccgtcctc ctccccgacc gcagcgcagg
     2401 ccgcgtctca gagcgcatcg gtgcgaggcc aaggcggcgc cgcgatcgag gtcacccccg
     2461 agttggccga ggcgctcgcc accgccggaa tcgatgtggc gacgctgtcc cccgaacaga
     2521 tcgccgaagc cacccagatg ctcgccgaga tgggtcgggt tcgccaggag atgctggcgg
     2581 tgccagcgtc ggagatcgtg gccaatcacc tgatggggat ctacgaactc ggagcgatcc
     2641 acctgggcga gaatcctccg aatttcgccg aggccaccat cgcgatcgag gcgctgcgag
     2701 cggtgttgga tcggctgtcg ggcaagttcg ccgacgcaga acccgtgctg cgtcaggcgc
     2761 tcacccaact tcagcaggtc ttcgtggagt tgaaggaaca ggtcgaggca cggccgggct
     2821 cacccgccga cgaatccgac gatcccgacg cctgaacaac ccacgccctt acgacgtgcc
     2881 ggcctacgtt cctgcggccg gcacgtccgc gtccgacgag catccggcac gcatc
//
LOCUS       DAOOMA010000331         2930 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_41322_length_2930_cov_2.875130, whole genome shotgun sequence.
ACCESSION   DAOOMA010000331 DAOOMA010000000
VERSION     DAOOMA010000331.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 2930)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2930)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2930
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41322_length_2930_cov_2.875130"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1065)
                     /locus_tag="PLP95_09105"
     CDS             complement(<1..1065)
                     /locus_tag="PLP95_09105"
                     /inference="COORDINATES: protein motif:HMM:NF015739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsQ-type POTRA domain-containing protein"
                     /protein_id="HPB45996.1"
                     /translation="MTSPSPSPSPDSPVNPTNASPVTNEDDELVIDLTTQELPAVERQ
                     ALLVGSGGIGYTTETFVAVDAAGVGASDTPVESEAPTRGPLGSDASERSALDLPLGAE
                     DGEAGFQAPPVDPRMIARRDGVATQRRRSKRRRIAMIVGTPLAVVALFVALHSPLIDV
                     DRITIVGSDSLDLAEVERVTGLALAESMITTRLGEVEAALGADPRFGRVAVERAWPNE
                     VRIRVTDRPLVAAVTNTERSFIVAKGGVVVREAHETDYIRRVLVHDDLDVEVGGALPP
                     AIGAAVDIVASLPFAVTAQIAEIEFSEAGELIFTLTNDATVLFGTVDKAPAKVSSLTT
                     MLTSNVDRTGVCQIDVRVPRA"
     gene            complement(1062..2081)
                     /gene="murB"
                     /locus_tag="PLP95_09110"
     CDS             complement(1062..2081)
                     /gene="murB"
                     /locus_tag="PLP95_09110"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: protein motif:HMM:TIGR00179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HPB45997.1"
                     /translation="MSASSMGDALDAVAADARARGLMVREHVPLGPFTTYRVGGAAAV
                     LVEVDTAEELAGLARILAGRSGGSADTDGHEDRGTDGVETLIVGNGSNLLVADGGAPV
                     VAIRLGAGFDRIEIDSTTVVAGGAARLPVVARRSVAAGLSGFEWAVGVPGSIGGAIRM
                     NAGGHGSDMAAVVRSVRVVDLRTGQDVSMPAAACEFSYRHSSIRPHQVVVEATLELRE
                     AHDGAAVAAGEELMSEIVQWRRTHQPGGHNAGSVFTNPAGDSAGRLIDTAGCKGLRIG
                     SAEVSTKHANFIQVDADGSANDVAEVMIAVIEAVRDRHGIQLHPETVMVGFDASVLER
                     IGGAR"
     gene            complement(2078..>2930)
                     /locus_tag="PLP95_09115"
     CDS             complement(2078..>2930)
                     /locus_tag="PLP95_09115"
                     /inference="COORDINATES: protein motif:HMM:NF019850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cyanophycin synthetase"
                     /protein_id="HPB45998.1"
                     /translation="VTNVEPAHLDHHGSWEDLLAAFRRFVAETDGPTVICADDPGAVL
                     LANASSRTLTYGTSTAADARILDERHTGSGSTWQVSLPDGSVVELSVDLPGHHLVLNA
                     TAALVLAWAVGIDPLAAARGIAGYRGVGRRFEERGAAGGVRFVDDYAHMPTEVRAVLS
                     AASSGPDREGRLVAVFQPHRYSRTASVWADYAGAFDAADVVVITEVYAAGEHPIDGVD
                     GALVLRAVREASPDLDVRWMATRAELIEGLTALLQPGDLCLTMGAGDLTTLPDQLIDQ
                     LTAQGRS"
BASE COUNT          472 a         1057 c          974 g          427 t
ORIGIN      
        1 ggccctcggg acccgcacgt cgatctgaca cacgccggtt cgatcgacat tggaggtcaa
       61 catggtggtc agcgacgaca ccttcgccgg agccttgtcg acggtgccga acagaacggt
      121 cgcgtcgttg gtcagggtga agatcaattc cccggcctcg ctgaactcga tctccgcgat
      181 ctgcgccgtc acggcgaagg gcagggacgc gacgatgtcg accgcggcgc cgatcgcggg
      241 aggaagcgcc ccgcccacct cgacatcgag gtcgtcgtgc accagaaccc gccggatgta
      301 gtcggtttcg tgcgcctcgc gaacgacgac gccgcccttc gcgacgatga acgacctctc
      361 ggtgttggtc accgcggcga ccaggggccg gtcggtcact cgaattcgaa cctcgttcgg
      421 ccacgcacgc tcgacggcga ccctgccaaa gcgcgggtct gcgccgagcg cggcctcgac
      481 ctcgccgagt cgggtggtga tcatcgactc tgcgagggcc aaaccggtga cgcgctccac
      541 ttctgccaga tcgagggaat cggaaccgac gatcgtgatg cgatcgacgt cgatcagcgg
      601 cgaatgaagg gccacgaaca gcgccacaac ggccagcggc gtgcccacga tcatggcgat
      661 gcgccgacgt ttggaccgtc ggcgttgtgt ggccaccccg tctcgacgag cgatcatacg
      721 cgggtcgacc ggcggcgcct ggaagcccgc ctcgccgtct tcggcgccca gcgggagatc
      781 gagcgcgctc cgctcggagg cgtcgctccc gagcggaccg cgggtcggcg cctccgactc
      841 gaccggggtg tcggaggcgc cgaccccagc ggcgtccacg gcgacgaacg tttcggtcgt
      901 atacccgatg ccgccggatc cgacgagcag cgcctggcgt tcgaccgcgg gcaactcctg
      961 ggtcgtgagg tcaatcacca actcgtcgtc ctcgttggtg accggggagg cattcgtcgg
     1021 gttgaccggc gagtcgggcg agggagaggg cgagggagag gtcaccgtgc ccctccgatc
     1081 cgttcgagta ccgaggcgtc gaacccgacc atcaccgttt cgggatgcag ttgtattccg
     1141 tgtcggtcgc gcaccgcctc gatcaccgcg atcatgacct ccgcgacgtc gttcgccgag
     1201 ccgtcggcat cgacctggat gaaattggcg tgcttggtcg acacctcggc gctgccgatg
     1261 cgcagtccct tacaaccggc cgtgtcgatc agccgtccgg cgctgtcacc ggccggattg
     1321 gtgaagaccg atccggcgtt gtgaccgccg ggctggtggg tgcgacgcca ctgcacgatc
     1381 tcgctcatca gttcctcccc cgctgcgacc gcggcgccat cgtgggcctc gcgcaattca
     1441 agggtcgcct cgaccacgac ctggtgcggc cggatgctcg agtggcgata cgagaactca
     1501 caggcggcgg cgggcatcga gacatcttgg ccggtgcgaa gatcgaccac gcggaccgac
     1561 cgcacgaccg cggccatgtc ggagccgtgg ccgccggcgt tcatccgtat ggccccgccg
     1621 atgctgcctg gcaccccgac ggcccactcg aaaccgctca gccccgcggc gacgcttcga
     1681 cgagccacga ccggcagccg cgccgcaccg ccggcgacca ctgtcgtcga gtcgatctcg
     1741 atacggtcga acccggctcc gaggcgaatc gccacgaccg gtgcgccgcc gtcggccacc
     1801 aacaggttgg aaccgttgcc gacgatcagc gtctcgacgc cgtcggtgcc gcggtcctcg
     1861 tggccgtccg tgtcggcaga cccacccgaa cgtccggcga ggattcgagc gagaccggcc
     1921 agctcctcgg cggtgtcgac ctccaccaga accgccgccg ccccgcccac tcgataggtg
     1981 gtgaatggac cgagcggcac gtgctcgcgc accatcaacc cgcgggctcg cgcgtcggct
     2041 gccaccgcgt ccaacgcgtc gcccatcgac gacgcgctca cgaccgcccc tgcgccgtca
     2101 gctgatcgat cagctgatcc ggaagggtcg tcaggtctcc cgcccccatc gtcaggcaca
     2161 ggtcccccgg ctgcaacagc gcggtcaacc cttcgatgag ctcggctcga gtcgccatcc
     2221 accgcacgtc cagatcgggg ctggcctcgc gcaccgcgcg aagcaccaag gccccgtcca
     2281 ccccgtcgat cgggtgctcc cccgcggcgt acacctcggt gatcaccacc acgtccgcgg
     2341 catcgaaggc ccctgcgtag tcggcccaca ccgaggcggt acggctgtag cggtgcggct
     2401 gaaagaccgc gacgaggcgg ccttcacggt cggggccgga cgacgccgcg gacaacaccg
     2461 cccgaacctc ggtgggcatg tgggcgtagt cgtcgacgaa ccgtacccct cccgcggcgc
     2521 cgcgctcctc gaaccgccgc ccgacgccgc ggtagccggc gatgcccctc gcggcggcca
     2581 acggatcgat accgacggcc caggcgagaa cgagcgccgc ggtcgcgttg agcacgaggt
     2641 gatgacccgg caggtcgact gagagttcca cgaccgaacc gtcgggaagc gagacctgcc
     2701 aggtactgcc ggatccggtg tggcgttcgt cgagaatccg cgcatcggcc gcggtactcg
     2761 tgccgtaggt cagcgtccgc gacgaggcgt tcgccaacag caccgccccc gggtcatccg
     2821 cacagatcac cgtcggcccg tcggtctcgg cgacgaatcg acgaaacgcc gcgagcaggt
     2881 cctcccagga gccgtggtgg tcgagatggg cgggttcgac gttggtgacg
//
LOCUS       DAOOMA010000332         2927 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_41389_length_2927_cov_3.989206, whole genome shotgun sequence.
ACCESSION   DAOOMA010000332 DAOOMA010000000
VERSION     DAOOMA010000332.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 2927)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2927)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2927
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41389_length_2927_cov_3.989206"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..378)
                     /locus_tag="PLP95_09120"
     CDS             complement(<1..378)
                     /locus_tag="PLP95_09120"
                     /inference="COORDINATES: protein motif:HMM:NF012899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HPB45999.1"
                     /translation="MILVVKVGTSSITDDDGRLDREAIAKVCRELAAARAAGHQVVLV
                     SSGAIAAGLPELGLSARHRPKDARTLQAVSAVGQARLMATYNECFDDIGGIVSGQVLL
                     TADDFHNRSTYVQARETLTRLLEL"
     gene            complement(375..1649)
                     /gene="obgE"
                     /locus_tag="PLP95_09125"
     CDS             complement(375..1649)
                     /gene="obgE"
                     /locus_tag="PLP95_09125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020378248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HPB46000.1"
                     /translation="MSNFVDEAALNVKAGDGGAGCVSFRREAHTPKGGPDGGDGGRGG
                     DIWLLADRNVASLLAFRDYPHRKATSGKHGSGARKHGASGDDLTVHVPEGTVVKDRDG
                     SVLADLVHHGDRWLAAEGGRGGKGNARFLSNRRRAPSFAEQGEVGEEKWVWMELKLMA
                     DVALVGLPNVGKSTLISRISAAKPKIADYPFTTLVPNLGVVRTDDDFEMIVADIPGLI
                     EGAAEGKGLGHQFLRHIERARVLAILVDLASVAELDPERQEEILLAELGRYQPDLLLR
                     ERVLLGARADLADPDTIDAFDGHVISSVTGRGVAEVIGKFRQAVELARSEIEEPEAYA
                     VYRPVPEGIRVGRNSYGEWEVIGREALRAVAVSDLTNPDALDYVHDRLGALGVDKALA
                     RAGARNGDVVNIGPMSFEYESGDEVNFADGGR"
     gene            complement(1714..>2927)
                     /locus_tag="PLP95_09130"
     CDS             complement(1714..>2927)
                     /locus_tag="PLP95_09130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB46001.1"
                     /translation="KVEITNSDAPVFFSRFFLNTVTIRRNAIGTYVLPVPMGSPKNFF
                     GTGVMAPAADRENMWAALNARCSPREQGDPFGSEYLNGWNGSAFFCPADPNPNYVDSP
                     GDQYEYMIYAPPGRTQPLVVHLYHPSVSGTEPGGLSAGGTPWSTRFRIYAPDNTPFDD
                     SDNPLATCTGSGETNPRTYSGAVSGTVSNWSGSTWSRFCTIPVSAPSGYYKLGVRNDD
                     ANTDAGSNPNTYSVLASYNGNGVRCDQRTDALCPTVQGRDWMSIYASVTSTAASFFLA
                     DVGSEHAGKQLEITLFDPGEGGQTIEILDPNGNPVRMDYYTTDGVFSGNNVASIDVSG
                     NSFPQSGRLSAYKFNDRYLVITVDLPNNYAATYGANTWWKVRYNFGGSNVNDRTTWSV
                     RVIGDPVHLSD"
BASE COUNT          495 a          952 c          972 g          508 t
ORIGIN      
        1 gagttcgagc agccgcgtga gcgtctcgcg ggcctgcaca taggtcgagc gattgtgaaa
       61 gtcatccgcc gtcaacagga cctgcccgga cacgatcccg ccgatgtcgt cgaaacactc
      121 gttgtaggtt gccatcaacc gcgcctgacc gaccgcggag acggcctgca acgtgcgtgc
      181 gtccttcggt cggtgacgcg cggagaggcc gagttcgggc aacccggcgg cgatggcccc
      241 ggaactcacg agcaccacct gatggccggc ggcgcgcgcc gctgcgagct cgcggcacac
      301 cttggcgatg gcctcgcgat cgagtcgccc gtcgtcgtcg gtgatggagg aggttccgac
      361 cttgacgacg agaatcatcg tccaccgtcg gcgaagttca cctcatcgcc tgactcatat
      421 tcgaagctca tgggaccgat gttcaccacg tcgccatttc gcgcacccgc ccgggccagc
      481 gccttgtcga cccccaacgc gccgaggcgg tcgtgaacat aatcgagggc gtcgggattg
      541 gtgaggtctg aaacggcgac cgcccgcaag gcttcacggc cgatcacctc ccactcgccg
      601 tagctgttgc ggccgacacg aatgccctcg ggcaccggcc gatacacggc gtaggcctcg
      661 ggctcctcga tctcggaccg ggccagttcg acggcctgac gaaacttgcc gatgacctcc
      721 gccacgccgc ggccggtgac ggaactgatg acgtggccgt cgaacgcatc gatcgtgtcg
      781 gggtcggcga gatctgcccg agcgccgagc aacacccgtt cgcgcaacaa cagatcgggc
      841 tggtagcgac ccagttctgc gaggaggatc tcctcttgtc gttcagggtc gagttcggcc
      901 accgaggcaa ggtcgacgag gatcgccagc acgcgggccc gttcgatgtg acgcaggaac
      961 tgatggccga gccccttgcc ctccgccgcg ccctcgatca ggccgggtat gtcggcgacg
     1021 atcatttcga agtcgtcgtc ggttcgaacg accccgaggt tgggcaccag ggtggtgaac
     1081 gggtagtcgg cgatcttggg cttggcggcg gagatacggc tgatgagcgt cgacttgccg
     1141 acgttcggca atccgacgag ggccacgtcg gccatcagct tcaactccat ccacacccat
     1201 ttctcctcgc ccacctcgcc ttgctcggcg aagctcggcg cacggcggcg attcgacagg
     1261 aacctcgcgt tgcccttgcc gccgcggccc ccctccgccg cgagccagcg gtcgccgtga
     1321 tgcacaaggt cggccaagac cgaaccgtcg cgatccttca cgacggtgcc ttccgggacg
     1381 tgcacggtca ggtcgtcgcc cgaggcgccg tgcttgcgag cccccgaccc gtgcttgccg
     1441 ctggtggcct tacggtgggg atagtcgcga aacgcaagca gggatgcgac gttgcggtcg
     1501 gcgagcagcc agatgtcgcc gccgcgaccg ccgtcaccgc cgtcggggcc gcctttgggg
     1561 gtgtgggcct cacgacgaaa ggacacgcac ccggcgccgc cgtcgccggc tttcacgttg
     1621 agggcggcct cgtcgacaaa gttggacact cggccagtgt aggaccgccg cggtgcggca
     1681 ttcgtcagcg tgcggcgagt cggaccggtc gtgtcagtcc gagaggtgga ccgggtcgcc
     1741 gatcacacgg accgaccagg tcgtgcggtc gttgacgttc gaaccgccga agttgtagcg
     1801 aaccttccac cacgtgttgg cgccgtaggt cgcggcgtag ttgttcggca ggtcgacggt
     1861 gatcaccaga tatcgatcgt tgaacttgta cgcgctgagg cggcccgact gcggaaacga
     1921 gttgcccgac acatcgatcg acgcgacgtt gttgccggag aacacgccgt cggtcgtgta
     1981 gtagtccatc cgcaccgggt tgccgttggg atcgagaatc tcgatcgtct gaccgccctc
     2041 acccgggtcg aacagggtga tttcgagctg tttgccggcg tgttccgaac cgacgtcggc
     2101 caggaagaag ctcgccgcgg tcgaggtgac cgatgcgtag atcgacatcc agtcgcgacc
     2161 ctgcaccgtc gggcacagtg cgtcggttcg ctgatcgcaa cgcacgccgt tgccgttgta
     2221 ggacgcgagc accgagtagg tgttggggtt gctgcccgcg tcggtgttgg cgtcgtcgtt
     2281 acgcacgccg agcttgtagt agcccgacgg cgccgacacc gggatcgtgc agaaccggga
     2341 ccaggtcgat ccggaccagt ttgacaccgt tccgctcacc gcaccgctgt aggttcgcgg
     2401 gttggtttca ccggagccag tgcacgtcgc gagcgggttg tcggagtcgt cgaacggcgt
     2461 gttgtcggga gcgtagatcc gaaagcgcgt cgaccacggc gtccccccgg cgctcagacc
     2521 gcctggttct gtacccgaca cgctcgggtg atacaggtgc accaccaggg gctgggtgcg
     2581 tcccggcggg gcatagatca tgtattcgta ctgatccccg ggggaatcga cgtagttggg
     2641 gttggggtcg gccgggcaaa agaacgccga tccattccac ccgttgagat actcggaccc
     2701 aaaggggtca ccctgttcac gcggcgaaca cctcgcgttg agggccgccc acatgttctc
     2761 gcggtcggcc gccggggcca taacccccgt accgaagaaa ttcttcgggc tccccatcgg
     2821 aaccggcagc acataagtgc cgatcgcgtt gcgccggatg gtcaccgtgt tcaaaaagaa
     2881 gcgcgagaag aacaccggcg cgtccgagtt ggtgatctcc accttga
//
LOCUS       DAOOMA010000333         2925 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_41438_length_2925_cov_3.894774, whole genome shotgun sequence.
ACCESSION   DAOOMA010000333 DAOOMA010000000
VERSION     DAOOMA010000333.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 2925)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2925)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2925
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41438_length_2925_cov_3.894774"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..748)
                     /locus_tag="PLP95_09135"
     CDS             complement(<1..748)
                     /locus_tag="PLP95_09135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HPB46002.1"
                     /translation="MPKAVGIDLGTTNSVVAALEAGDPVVIPNSEGARTTPSVVAFTK
                     DGEVLVGEVAKRQAITNPDRTIRSVKRHMGTNWTIGIDGKDYTSQEISARTLMKLKRD
                     AEAYLGDAVTQAVITVPAYFDDAQRTATKEAGQIAGLEVLRIINEPTAAALAYGMEKE
                     GEDEAILVFDLGGGTFDVSVLEIGDGVFEVKSTHGDTELGGDDWDQRIIDWLVEGFKN
                     SQGVDLAKDNMAMQRLKEAAEKAKIELSQVQ"
     gene            1054..1815
                     /gene="gpmA"
                     /locus_tag="PLP95_09140"
     CDS             1054..1815
                     /gene="gpmA"
                     /locus_tag="PLP95_09140"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000301556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HPB46003.1"
                     /translation="MSDLILLRHGESEWNRLNLFTGWYDCDLTDTGRAEASAAGATLL
                     DAGLGPTVLHTSLQKRAIRTAQLALDTANLMWLPVRRSWRLNERHYGDLTGRNKAETT
                     EMYGEEQVKIWRRSYDIAPPAIGSDNETNPNDDAQYASLPADIIPAAECLKDVVARLM
                     PYWFDSITTDLAAGHTVLVVAHGNSLRALVKHLDSIEDADITELNIPTGIPLHYELGD
                     DFRPREAKPTSQRYLGDADAIAAKAAAVAAQATAK"
     gene            1902..2666
                     /locus_tag="PLP95_09145"
     CDS             1902..2666
                     /locus_tag="PLP95_09145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPB46004.1"
                     /translation="MTEEAVSDTQTWSTTVAASAVDATKEYGSGDTRVVALDKVTASF
                     NRGQFTAIMGPSGSGKSTLMHCLAGLDVLTSGTIRVGNDDLSTLSDRELTLLRRDKIG
                     FIFQAFNLIPTLTALENITLPMDLGGTKPDKAWLDTVIDTVGLRERLTHRPSELSGGQ
                     QQRVAVARALASRPQIIFADEPTGNLDSRTGTEILTFMRKAVDELRQTIVMVTHDPNA
                     AGHADRVLFLADGQLVDEMFEPTAAKVLDKMKALGD"
     gene            2731..>2925
                     /locus_tag="PLP95_09150"
     CDS             2731..>2925
                     /locus_tag="PLP95_09150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB46005.1"
                     /translation="MRLVAAVLDFSVLMVVMLVLQLSWTTERGGETVPTSASTWISVG
                     VAVAYFVVSHAVIGQTLGKRM"
BASE COUNT          532 a         1005 c          858 g          530 t
ORIGIN      
        1 actgaacctg gctcagttcg atcttggctt tctccgccgc ctccttgaga cgctgcatcg
       61 ccatgttgtc ctttgccagg tcgacgccct gggagttctt gaacccctcg accaaccagt
      121 cgatgatccg ctgatcccag tcgtcgccgc cgagttcggt gtcaccgtgg gtcgacttca
      181 cctcgaaaac accgtcgccg atctccagca ccgacacatc gaaggtgccg ccgccgaggt
      241 caaacaccag gatcgcctcg tcttcaccct ctttttccat gccgtacgcg agcgctgccg
      301 ccgtgggctc gttgatgatg cgcagcactt caagcccggc gatctgaccg gcctctttgg
      361 tggcggtgcg ctgcgcatcg tcgaaatagg ccggcacggt gatgaccgcc tgggtcaccg
      421 catcaccgag atacgcctcg gcgtcgcgct tgagcttcat cagcgtgcgt gccgagattt
      481 cctgggacgt gtagtccttg ccgtcgatgc cgatggtcca gttggtgccc atgtgacgct
      541 tgaccgaccg aatcgtacgg tccgggttgg tgatggcctg gcgcttggcg acctccccga
      601 cgagcacctc accgtccttg gtgaacgcga ctaccgacgg cgtggtgcgg gcgccttcgc
      661 tgttggggat gacgaccgga tcgccggctt ccaacgccgc gaccaccgag ttggtcgtac
      721 ccagatcaat tccgactgcc tttggcattt ggggggtctc ctctcacggc gtggcgccgc
      781 tgtttggttg cggcctcgtg gccgaaatca tgatgttgcc gccaagctta gcggcctctc
      841 gaccggttca ccctgcagct gagtcattta gatcataaac cttgagtcac tcgctatcaa
      901 cttttatcca caggtcgact taatgcattc tcaggcaccc ctccgcacgc cgcgccgcat
      961 cgatgtcccc catcgctggg tgtgcagaat ttccgtcgag ccgtcggttg gctcatcatg
     1021 gagacccacc gacgatcgag agcgagggac cccatgtccg atctcatcct gcttcgccac
     1081 ggcgaaagcg aatggaaccg actgaacctg ttcaccggtt ggtacgactg cgacctcacc
     1141 gacaccggac gagcagaggc atccgcggcc ggtgccacct tgctcgacgc cggcctgggt
     1201 ccgacggtcc tgcacacgtc cctgcaaaag cgcgccattc gtaccgccca gctcgcgctc
     1261 gacaccgcga atctgatgtg gttgcccgta cgtcgttcgt ggcggctgaa cgagcggcac
     1321 tacggcgacc tcaccgggcg caacaaggcc gagaccaccg agatgtacgg cgaggaacag
     1381 gtcaagatct ggcggcgctc ctatgacatc gctccgcccg cgatcggttc ggacaacgag
     1441 acgaacccca acgacgacgc acagtatgcg tcactgcccg ccgacatcat ccccgccgcg
     1501 gagtgcctca aagacgtcgt ggcccggctc atgccgtatt ggttcgactc gatcaccacc
     1561 gacctcgccg cggggcacac ggtgctggtg gtggcccacg gcaactcatt gcgagcgttg
     1621 gtgaagcacc tcgactcgat cgaggacgcc gacatcaccg aactcaacat ccccaccggc
     1681 attccgctgc actacgaact cggcgacgac ttccggcctc gcgaggccaa gccgacctcg
     1741 cagcggtacc tgggcgatgc ggacgccatc gccgcgaagg ccgccgccgt ggcggcccag
     1801 gcgacggcga agtagccaaa cgtcggccag cgtggcgctc ggggacgatt cactgcccgc
     1861 acccgagcgc ccgaagccga taccgtgtca ccactcggac gatgacggag gaagccgtga
     1921 gcgacaccca gacctggtca accaccgtcg ccgcatcagc ggtcgacgcg accaaggaat
     1981 acggttcggg cgacacgcgt gtcgtcgcac tcgacaaggt gaccgcctcg ttcaaccgcg
     2041 gtcagttcac cgcgatcatg ggaccatccg gttctggtaa gtccacgctg atgcactgcc
     2101 tcgccggcct cgacgtgttg acatcgggca cgatccgcgt cggcaacgac gacctttcaa
     2161 ccctgtccga tcgggagctc acgctgttgc ggcgcgacaa gatcggcttc attttccagg
     2221 cgttcaacct catcccgacg ctcaccgcgc tcgagaacat caccctgccg atggacctcg
     2281 gaggaaccaa gcccgacaag gcgtggctcg acacggtgat cgacaccgtc gggctgcgcg
     2341 aacgcctcac ccatcgcccc tcagaattgt cgggcgggca acagcagcgt gtcgccgtcg
     2401 cacgggcgct cgcatcacga ccgcagatca tcttcgccga cgagccgacc ggtaacctcg
     2461 acagtcgaac cggcaccgag atcctcacct tcatgcgcaa ggcggtcgac gaactgcgtc
     2521 agaccatcgt catggtgacc catgatccga acgcggcagg tcacgccgac cgagtgctgt
     2581 ttctcgccga tggccaactc gtcgacgaga tgttcgagcc gacggccgcc aaggtgctcg
     2641 acaagatgaa ggcgctcggc gactgatcag ggcacccgga cccgagtgaa caacgaaccg
     2701 acggtcgaac tgcatccttg ctcagcggta atgcgcctcg ttgccgcggt gctcgacttt
     2761 tcggtgctga tggtcgtgat gctggtccta caactgtcgt ggaccaccga acgcggcggc
     2821 gaaaccgttc ccacgagtgc ctcgacatgg atctcggtcg gcgtggccgt cgcctacttc
     2881 gtcgtctccc acgcggtcat cggacagacg ttgggcaagc ggatg
//
LOCUS       DAOOMA010000334         2924 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_41466_length_2924_cov_2.849773, whole genome shotgun sequence.
ACCESSION   DAOOMA010000334 DAOOMA010000000
VERSION     DAOOMA010000334.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 2924)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2924)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2924
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41466_length_2924_cov_2.849773"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..336)
                     /locus_tag="PLP95_09155"
     CDS             complement(<1..336)
                     /locus_tag="PLP95_09155"
                     /inference="COORDINATES: protein motif:HMM:NF019845.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HPB46006.1"
                     /translation="MKAWFLSESPGEYRWGDVPTPQPGPGEIRVRIVASAVNHIDHWQ
                     TQGRPKPKSFPHVPGSDGAGVVDAVGEGVSRLAVGDEVAINAAITSREAVETLGIDNV
                     FDPSLQLLGE"
     gene            complement(336..959)
                     /locus_tag="PLP95_09160"
     CDS             complement(336..959)
                     /locus_tag="PLP95_09160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB46007.1"
                     /translation="MISPGQPWGNLVELDGDEPRAHDDRGLAELLARHPGARVLLDGG
                     DLFTSLGGQAPAEPFEMPIDLLEVTADGRQHLAAAHVVARRRGWRGHFVVAMNGTHLG
                     GWNLGPKAHPNDGIVDLTSGSLGLGDRLKARRRAPAGAHLPHPGLATSRRRSLELHLA
                     TATPLFIDGVKVGRVSHLVIAVRGDAGRIVLAPPMRAGSSAGPVQST"
     gene            complement(956..>2924)
                     /locus_tag="PLP95_09165"
     CDS             complement(956..>2924)
                     /locus_tag="PLP95_09165"
                     /inference="COORDINATES: protein motif:HMM:NF019758.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HPB46008.1"
                     /translation="GGSAYGARGGRYLPPQRLEVIERLTSEDLLPAIYFIFSRNACDD
                     ALARCRDAGLRFTDASEAARIRTIVEAAVEGLGDHDLEVLGYDLWLQALQQGIASHHA
                     GMIPAFKEAVERCFIEGLVKVVFATETLALGVNMPARTVVIEKLTKYNGESHDFLTPA
                     QFTQLTGRAGRRGIDTEGYSVLLWSPFVGFGQVAGLATSREYPLTSAFRATYNMSANL
                     VARMSRPEALSVLGRSFAQFQSDRAIVGFERRLARSKEARAALDAALRCSCGDIGEYA
                     DLVQRVTSQRRHRPDGSAAIARSAELLRPGDVIVASFGDEAAQRLELSPGAGLRLAVV
                     SVAARGKGRLRVRTIDEQGRPVTLSPDDLAEPVRSIDTIEFPQPYAPRDPDFLDAVAA
                     ALVDARSTAATTAPAGPSRWHRLKARMETHPVHRCRRRDEHLEAWGEAASLDATIEQL
                     STQLDRRSNTVARRFDAICDLLAGLDCLDGWALTERGERLAQIYHECDLLVTLALDEG
                     AFDGLTVPELTAIVSAIVYEERRPERRGVAAFPNNTTRRRFNRFERLANVIEKAERSR
                     GLPLSRRPDPGFMIKAHGWASGRDLGTLIDDDDMSGGDFVRTTKMLIDVMGQLAEVAP
                     DLGTRRCAGDAIKAIKRGVVADAGAVTPDGP"
BASE COUNT          446 a          975 c         1038 g          465 t
ORIGIN      
        1 ctctccgaga agctgcaacg acgggtcgaa cacgttgtcg atgccgagcg tctcaaccgc
       61 ctcgcggctg gtgatggcgg cgttgatcgc cacctcgtcg ccgacggcga gccggctgac
      121 cccctccccc accgcatcca cgacgccggc gccgtccgaa cctgggacat gaggaaacga
      181 cttcggcttc ggccgaccct gggtctgcca gtggtcgatg tggttgaccg cgctcgcaac
      241 gatccgcaca cgaatctctc cgggtccggg ttgtggggtg ggcacgtctc cccagcggta
      301 ttcccctggc gactccgata agaaccaagc cttcatcagg tggactgtac cggtcccgcg
      361 ctagaaccgg cgcgcatcgg cggggccaac acgatccgac cggcatcgcc gcgaaccgcg
      421 atcacgagat ggctcacacg gccgaccttc acgccgtcga taaacagcgg ggttgcggtc
      481 gcaagatgca actccaggct gcggcgccga ctggtcgcga ggccgggatg cggcaggtgt
      541 gcaccggcgg gcgctcggcg ccgtgctttg aggcggtcgc cgaggccgag tgacccgctg
      601 gtcagatcga cgatgccgtc gttggggtgc gccttcggac ccaggttcca gccgccgagg
      661 tgggtgccgt tcatggcgac cacgaagtgc ccgcgccatc ctcgccgtct ggccacgaca
      721 tgggcggcgg cgagatgttg gcgcccgtct gcggtcacct ccaacaggtc gatcggcatc
      781 tcgaagggct cggcgggcgc ctggccgccg agggaggtga acaggtctcc gccgtcgagc
      841 agcactcggg cgccggggtg tcgggcgagg agctcggcca acccgcgatc gtcgtgggcc
      901 cgcggctcgt cgccgtccaa ctcgacgagg ttgccccacg gctgtccggg gctgatcacg
      961 ggccgtcggg cgtcaccgca ccagcgtcgg cgacaacccc tcgtttgatg gccttgatcg
     1021 cgtcgccggc gcagcggcgc gtcccgagat ccggggcgac ctcggcgagc tggcccatca
     1081 cgtcgatgag catcttggtg gtgcgcacga aatcgccgcc cgacatgtcg tcatcgtcga
     1141 tcagggtgcc caggtcgcgg cccgaggccc agccatgggc tttgatcatg aaccccgggt
     1201 cgggccgccg agacagcggt aggccgcggg aacgctcggc tttttcgatg acgttcgcca
     1261 ggcgctcgaa ccggttgaac cgccgccggg tcgtgttgtt cggaaaggcg gcgaccccac
     1321 ggcgttccgg gcggcgttcc tcatacacga tcgccgaaac tatggcggtg agctcgggca
     1381 cggtgagtcc gtcaaatgcc ccttcgtcga gggcgagggt caccagcaga tcgcactcgt
     1441 ggtagatctg ggccagccgt tcaccgcgtt cggtcagggc ccagccgtcg aggcaatcga
     1501 gccctgccag aaggtcacag atggcgtcga aacgacgtgc gacggtgttg gatcgccggt
     1561 cgagttgagt cgagagctgt tcgatcgtcg cgtcgagcga cgccgcctcg ccccacgctt
     1621 cgaggtgttc gtcgcggcga cggcaccggt gcaccggatg ggtttccatg cgggccttga
     1681 gccggtgcca ccgcgagggc ccggcggggg ccgtcgtggc ggcggtcgac ctcgcgtcga
     1741 cgagtgccgc cgcaaccgcg tcgaggaaat cgggatcgcg cggtgcgtag ggctgtggga
     1801 actcgatcgt gtcgatggac cggaccggct cggcgaggtc gtcgggtgac agcgtgaccg
     1861 gacggccctg ttcgtcgatg gtgcgcaccc gcagtcggcc ctttccgcgg gcggcgaccg
     1921 acaccacggc gaggcgaagg ccggccccgg gcgacaattc gagtcgctgc gccgcttcgt
     1981 cgccgaaact cgcaacgatg acgtctccgg gccggaggag ttcggcggat cgcgcgatcg
     2041 ccgcgctgcc gtcggggcgg tgtcggcgtt gcgaggtcac ccgttgcacc aggtcggcgt
     2101 actcgccgat gtctccacag gagcaccgca gcgcagcgtc gagagctgcc cgtgcctcct
     2161 tggaacgcgc cagtcgccgt tcgaatccga cgatcgcacg gtcggattgg aactgggcga
     2221 aactgcgccc gagcaccgac agcgcctcgg gtcggctcat acgcgccacg aggttcgccg
     2281 acatgttgta cgtggcacgg aaggcgctgg tgagcggata ctcgcggctc gtcgccaggc
     2341 cggcgacctg gccgaagccg acgaacggcg accacaagag caccgaatac ccctcggtat
     2401 cgatcccgcg gcgcccggca cggcccgtga gctgggtgaa ttgcgcgggg gtcaggaaat
     2461 cgtggctctc gccgttgtac ttggtcagct tttcgatgac gacggtgcgc gccggcatgt
     2521 tcacaccgag cgccagggtt tcggtggcga acaccacctt cacgagcccc tcgatgaagc
     2581 accgctccac ggcttccttg aaggcgggga tcatgccggc gtgatggctg gcgatgccct
     2641 gttgcagcgc ctggagccac aggtcgtaac ccaagacctc gaggtcgtgg tctccgaggc
     2701 cctcgacggc ggcctcgacg atggtgcgga tccgagccgc ctccgacgcg tcggtgaagc
     2761 gcaggccggc gtcgcggcac cgggcgagcg catcgtcgca ggcgttgcgg ctgaagatga
     2821 agtagatcgc cggaagcagg tcttcgctcg tgaggcgttc gatcacctcg agtcgctggg
     2881 gtggaaggta gcggcctccg cgcgccccgt aggcgctgcc accg
//
LOCUS       DAOOMA010000335         2923 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_41485_length_2923_cov_3.615411, whole genome shotgun sequence.
ACCESSION   DAOOMA010000335 DAOOMA010000000
VERSION     DAOOMA010000335.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 2923)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2923)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2923
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41485_length_2923_cov_3.615411"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            137..937
                     /locus_tag="PLP95_09170"
     CDS             137..937
                     /locus_tag="PLP95_09170"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019846.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HPB46009.1"
                     /translation="MDVTHDAAMSPPDPTRYGRSFADVYDDWYHELPTDDLVGFIRSR
                     VPAPAAVLELGVGTGRVALALASAGYKVTGMDSSEQMLERLALNDPQGRVRPLLADAA
                     DPNAHPRADVVVAALNMIFNLTTRVAQTNCLRGCAKAIGSDGILVTETIVAARLTERR
                     TELVTRSVEPGRVVLIATDARPETRPGAGAAGEADLGAALVHGSHLEFTDGGVALRPW
                     TVRTISPAHLDELATTAGLELVERFEDFAENAFDDGSRSQVSVYRRAR"
     gene            927..1391
                     /locus_tag="PLP95_09175"
     CDS             927..1391
                     /locus_tag="PLP95_09175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB46010.1"
                     /translation="MPADRFTVSQLVRAPADVVWDDLAHIDRHVDWMYDAADITFHGE
                     QRRGVGTTFDCLTVVGPLRTVDRMEITSWVEGAQIGVRHVGLVTGEGVISVIDAGPSH
                     CVVSWNERLRFPWFVGGPVATFAARPVMVAIWRSSLRKLAARWPSALPDGEQ"
     gene            complement(1371..2279)
                     /locus_tag="PLP95_09180"
     CDS             complement(1371..2279)
                     /locus_tag="PLP95_09180"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HPB46011.1"
                     /translation="MSSPPPFPPGPPSSAGFGGGVPATDRVCYRHHDRRAGVSCQRCN
                     RPICPSCMVSASVGFHCPDCAKAGAAGSRTISARDLAPTSYLTLTLVAVNIAIFFIDR
                     AGIGVNSRALSQEWVLYEPFVARGQWWRLVMSGFLHANLMHLAFNMFALYSIGTFLEK
                     VVGPLRYGLIYAVSLIGGSLGVMLLSDAAPTVGASGAIFGIFGAFAVLSLSRGISPMA
                     SGIGSTILLNLFITFTVPGISIGGHVGGLLAGAACGAMLFGVNPDQARDRRARQQRVG
                     NDWAVLGSMAALGAVCFVASLLLAVG"
     gene            complement(2330..>2923)
                     /locus_tag="PLP95_09185"
     CDS             complement(2330..>2923)
                     /locus_tag="PLP95_09185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB46012.1"
                     /translation="LSDEAADVLEHSLSRLVYADDLGGFIRVHNNLDLSNVERIRSGW
                     LVHRFGCRAQSDLELESRPLSPLVDLAGLLHGCSTVARSAAADALAAINPDEQPEPGT
                     PAALELEADRREVRVLADAWEERAADALIAGYTSNDAVHRLLPVERIARDALLTLFEL
                     ELSIRDLVVSTLRGHQLVSLPLESVDGLIDTPIRLRW"
BASE COUNT          529 a         1050 c          920 g          424 t
ORIGIN      
        1 gaggcgacac tgcgcatcgg cgtcgatgcc gcatgatcga gatagacctc cggggcgttc
       61 accttgcaga ggttaggacc cggcgaccgc gtccccggca ccccgacgtt gccgtggcat
      121 ctgtcagact gacgccgtgg atgtcactca cgacgctgcc atgtcgccgc cggatccgac
      181 ccgttacggc aggagcttcg cggacgtcta cgacgactgg taccacgagt tgcccaccga
      241 cgacctcgtt ggtttcatcc ggtcacgggt gccggcgccg gcggcggtgc tcgaactcgg
      301 ggttggaacc ggacgcgtcg cgctcgcgct ggcgagcgcc ggctataagg tcaccggcat
      361 ggactcgtcg gagcagatgc tcgaacggct cgcgctcaac gatccgcaag gtcgcgtacg
      421 ccccctcctc gcggatgccg ccgaccccaa cgcccacccc cgagccgatg tggttgtcgc
      481 cgcgctcaac atgatcttca acctcacgac cagggtcgct cagaccaact gccttcgagg
      541 gtgcgccaaa gcgatcggct ccgacggaat cctcgtcacc gagaccatcg tggcggcgag
      601 gctcaccgaa cggcgcaccg agttggtcac ccgttcggtc gaacccgggc gcgtcgtcct
      661 catcgccacc gacgcccggc ccgagacgcg ccccggcgca ggagccgctg gggaggcgga
      721 cctcggcgca gcgctcgtgc acggcagcca cctcgagttc accgacggcg gggtcgcgct
      781 tcgcccctgg acggtacgca ccatcagccc cgcgcatctc gacgaattgg ccacgaccgc
      841 cggcctcgaa ctcgtcgaac gattcgaaga tttcgccgag aacgccttcg acgacgggtc
      901 ccgctcacag gtgtcggtgt atcgccgtgc ccgctgaccg cttcaccgtc tcccaactcg
      961 tccgcgcccc cgccgatgtg gtctgggacg acctcgcgca catcgatcgc cacgtcgact
     1021 ggatgtacga cgccgccgac atcaccttcc acggcgaaca gcgacgcggc gtcggcacca
     1081 ccttcgactg cctcaccgtc gtgggtccgc tgcgcaccgt cgaccgcatg gagatcacct
     1141 cctgggtcga gggcgcgcag atcggggttc gccacgtcgg cctcgtcacc ggcgaagggg
     1201 tcatcagcgt catcgacgcc ggcccgtcac attgcgtcgt gagctggaac gagcgcctcc
     1261 ggtttccgtg gttcgtcggc ggcccggtcg caacgttcgc ggcccgcccc gtcatggtcg
     1321 ccatctggcg atcgtcgctg cggaagctcg cggcccgatg gcccagtgcg ctacccgacg
     1381 gcgagcagta gcgacgccac gaaacacacc gcgcccaacg ctgccatcga gccgagcacc
     1441 gcccagtcgt tgcccacccg ttgttgacgc gctcggcgat cgcgagcctg atcggggttc
     1501 accccgaaca gcatcgcacc gcatgcggcg ccggcgagca gaccgccgac gtgaccaccg
     1561 atggagatcc ccggcaccgt gaaggtgatg aacaggttca acagaatcgt gctgccgatg
     1621 ccgctggcca tcgggctgat cccgcggctc aggctcagca ccgcgaaggc cccgaaaatg
     1681 ccgaagatcg cgcccgaagc cccgaccgtc ggagccgcgt cggacagaag catcacgccg
     1741 agcgatccgc cgatcaacga cacggcatag atcaacccgt agcgaagcgg cccgaccacc
     1801 ttttcgagaa acgtcccgat cgagtacagc gcaaacatgt tgaacgcgag gtgcatcagg
     1861 ttcgcatgca ggaacccgct catcacgagg cgccaccact gcccgcgagc gacgaacggt
     1921 tcgtacagca cccactcctg tgacaatgcg cgggagttca ccccgatgcc ggcccggtcg
     1981 atgaagaaga tcgcaatgtt gaccgcgacg agcgtcaagg tcagatacga ggtcggggcg
     2041 aggtcgcggg cgctgatggt ccgagacccg gccgcacctg ctttcgcaca gtccgggcag
     2101 tgaaacccga ccgacgcact caccatgcac gacgggcaga tcggacggtt gcagcgctga
     2161 cacgacaccc cggcccggcg gtcgtgatgt cgataacaca cccgatcggt cgccgggact
     2221 cctccgccga atccagcaga tgacggcggt ccggggggaa acggcggagg cgacgacatc
     2281 gtcacagcct accgatcgtg cgtgcccgga attgggcggc gcatcagggt caccaacgca
     2341 accggatcgg ggtgtcgatg aggccgtcga ccgactcgag cggcaacgag accagctgat
     2401 ggccacgcag cgtcgacacg accagatccc ggatcgacaa ctcgagctcg aacagcgtca
     2461 gcagcgcgtc ccgggcgatt cgttccaccg gcaacaggcg gtgcaccgcg tcgttggagg
     2521 tgtaaccggc gatcagcgcg tcggcggcgc gctcctccca ggcgtcagcg aggacccgca
     2581 cctcgcgacg atccgcctcg agttcgagcg ccgccggggt tcccggctcc ggttgctcat
     2641 cagggttgat cgctgcgagt gcgtccgcag cagcggatcg cgccacggtg ctgcatccgt
     2701 gcaacagccc ggccaaatcg accagcggcg acagcggcct cgactcgagt tcgaggtcgg
     2761 attgcgcccg gcatccgaac cggtgaacca accacccgga gcggatccgt tcgacattgc
     2821 tcagatcgag gttgttgtgc acccggatga acccgccgag gtcatccgcg tacaccaacc
     2881 gcgacagcga atgctccaac acatccgccg cctcgtccga cag
//
LOCUS       DAOOMA010000336         2919 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_41545_length_2919_cov_4.152584, whole genome shotgun sequence.
ACCESSION   DAOOMA010000336 DAOOMA010000000
VERSION     DAOOMA010000336.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 2919)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2919)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2919
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41545_length_2919_cov_4.152584"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..279)
                     /gene="nuoK"
                     /locus_tag="PLP95_09190"
     CDS             complement(<1..279)
                     /gene="nuoK"
                     /locus_tag="PLP95_09190"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015439978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HPB46013.1"
                     /translation="MIGSNAYLMLSAVLFAIGVAGLLIRRNPLVMFMCIELMLNAVNL
                     SFVAFGNSLNDIGGQVIVFFVLVVAAAEVTVGLGLIVAIMRRNTGADAD"
     gene            complement(276..902)
                     /locus_tag="PLP95_09195"
     CDS             complement(276..902)
                     /locus_tag="PLP95_09195"
                     /inference="COORDINATES: protein motif:HMM:NF012709.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HPB46014.1"
                     /translation="MLAVQAGQLITFVIASMITIGGAVGVIAFRNPVHNALSLIATLF
                     GMAVLYAAQGAYFVAAIQVIVYAGAIVVLFVFVIMLLGVDRIQSYAKERTPRTLLIGG
                     SLGAGVAVMVLIAILGGIPKPTGAESVQGALGATDVNAIGEAMFSTYIFAFEITSLLL
                     VVAIVGAVVLARKPKGEPIDLDEFPKPEIDLSDADEQDALDAEIGAQQ"
     gene            complement(902..1600)
                     /locus_tag="PLP95_09200"
     CDS             complement(902..1600)
                     /locus_tag="PLP95_09200"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015439976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit I"
                     /protein_id="HPB46015.1"
                     /translation="MGVLGGFAVTLKKMFKHTDTGRVLTLEYEGGRGGAKDQKRDKPE
                     RLHGRHVLNRYEDGMEKCIGCELCAGVCPAKCIYVRGADNDPENPTSPGERFGFVYEI
                     NYLRCIHCDMCVEACPTEAITESKLFEFSFTSREDAIYTKDELLVGDDGKPKQLPWED
                     WREGDDVNTSAWMRATSPGGDARFENMVAWSGELGYGVRAPEGGQPLEREQAAQAAAA
                     AHDDHHDDHGGGHH"
     gene            complement(1600..2799)
                     /gene="nuoH"
                     /locus_tag="PLP95_09205"
     CDS             complement(1600..2799)
                     /gene="nuoH"
                     /locus_tag="PLP95_09205"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020379280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HPB46016.1"
                     /translation="MIALDPLLNNLDLTGVLVMLAKVLVAFGILMTSVILMIWFERKL
                     HADFSNRLGPRWAGPFGVLQTLADGMKIMFKSDLIPDQADRAVFKYAPLLSLVPAFIV
                     FSVIPIGGNFNGDKAGMVEIAGRQTMMQVADPHMGVLVILAASSLAIYGIMLAGWSSG
                     SKYPLLGSVRASAQMVSYEAALGLSVLAVLLNSESLSTNAIVNSQSTWGWNAWATGVI
                     PFVIFVIAATAEMNRPPFDLVEAEQELVGGFHTEYAGFRFAIFYLAEFMNVVTMSTII
                     VTLFLGGPSGPDFGVTGWVSDYVFPVIWFLLKTLFMLVVFVQFRTTLPRLRYDQLMDL
                     GWKIMIPLALGWFLLLAGLKVAQDEGWNQFVVGLVSVLVIFVGAALLSVAVRTARYRR
                     EVEKVGA"
BASE COUNT          593 a          973 c          891 g          462 t
ORIGIN      
        1 atcggcgtcg gcaccggtgt tgcggcgcat gatcgccacg atcagaccga ggccgaccgt
       61 gacctccgcc gcagcgacga cgagtacgaa aaagacgatg acctggccgc cgatatcgtt
      121 cagcgaattg ccgaaggcca caaagctcag gttcaccgcg ttgagcatca actcgatgca
      181 catgaacatc acgagcgggt tgcgtcggat gagcaggccc gcgacaccga tcgcgaacag
      241 caccgccgac aacatcagat atgcgttgct accaatcact gctgggcccc gatctcggcg
      301 tcgagtgcgt cctgttcgtc ggcgtcgctg aggtcgatct ccggcttggg gaactcgtcg
      361 agatcgatcg gctccccctt cggcttgcgg gccaacacca ccgcacccac gatcgcgacc
      421 accaacagca acgaggtgat ctcgaacgcg aagatgtagg tgctgaacat cgcctcgccg
      481 atggcgttga cgtcggtggc accgagcgcc ccctgaaccg attccgcccc ggtcggtttc
      541 gggattccgc cgaggatggc gatcagcacc atcacggcca cgcccgcgcc gagggatccg
      601 ccgatgagca gtgtgcgagg cgtccgctcc ttggcgtagc tctgaatccg gtcgaccccg
      661 agcaacatga tcacgaacac gaacaacacc acgatcgcac cggcgtacac gatcacctgg
      721 atcgcggcga cgaaataggc accctgggcc gcgtacaaca cggccatgcc gaacagcgtc
      781 gcgatgagcg acagcgcgtt gtggacggga ttgcggaagg caatcacccc gaccgcccca
      841 ccgatggtga tcatcgaggc gatgacgaag gtgatgagtt gccccgcttg aacggcgagc
      901 atcagtggtg acctcccccg tggtcgtcgt ggtggtcgtc gtgtgccgcg gcggccgcct
      961 gagcggcctg ctcgcgctcg agcggctggc caccctcggg ggcgcgcacg ccgtagccga
     1021 gttcacccga ccaggccacc atgttctcga aacgggcgtc gccgccgggc gaggtggccc
     1081 gcatccacgc cgaggtgttc acgtcgtcac cctcgcgcca gtcttcccac ggaagctgct
     1141 tgggtttgcc gtcgtcgccg acgagcagct catccttggt gtagatcgcg tcctcgcggc
     1201 tggtgaagct gaactcgaac agcttcgact cggtgatcgc ctcggtcggg cacgcctcga
     1261 cgcacatgtc gcaatggatg cagcgcaggt agttgatctc gtagacgaac ccgaagcgct
     1321 cacccggcga ggttggattc tccggatcgt tgtcggcgcc gcgcacatag atgcacttgg
     1381 cggggcacac accggcgcac agctcgcaac cgatgcactt ctccatcccg tcctcatagc
     1441 ggttgaggac gtgacgaccg tgcagacgct cgggcttgtc gcgcttttgg tccttggctc
     1501 cgccgcgacc gccctcgtat tcgagggtga gcacacgacc ggtgtcggtg tgcttgaaca
     1561 tcttcttcaa cgtgaccgcg aagccaccga gcacacccat cacgcaccaa ccttttcgac
     1621 ctcgcgacgg tagcgagccg ttcgcaccgc caccgacaac agcgccgccc caacgaagat
     1681 caccaacacc gacacgaggc cgacgacgaa ctggttccaa ccctcgtcct gggcgacctt
     1741 gagcccggcg agcaacagga accatccgag cgcgagcggg atcatgatct tccaaccgag
     1801 atccatgagc tggtcataac gcaggcgggg cagcgtcgtg cggaactgca cgaagaccac
     1861 caacatgaac agcgtcttga gcaggaacca gatcaccggg aacacgtagt ccgacaccca
     1921 gccggtgacc ccgaagtccg ggcccgacgg gccgccgagg aacaacgtga cgatgatcgt
     1981 cgacatcgtc accacgttca tgaactcggc cagatagaag atggcgaaac ggaatccggc
     2041 gtattcggtg tggaacccgc cgacgagttc ctgttccgcc tcgacgaggt cgaacggggg
     2101 tcggttcatc tcggcggtcg ccgcgatgac gaagatgacg aacgggatga ccccggtcgc
     2161 ccaagcgttc caaccccacg tcgactggct gttgacgatc gcattggtcg acagcgactc
     2221 ggagttcaac aacaccgcca gcaccgacag cccgagcgcc gcctcatacg acaccatctg
     2281 cgccgaggca cgaaccgagc cgagcagcgg atacttcgac cccgacgacc agccggccaa
     2341 catgatgccg tagatggcca gggaactcgc cgccaggatc acgagcacac ccatgtgcgg
     2401 gtcggccacc tgcatcatcg tttgacgacc ggcgatttcg accatgccgg ccttgtcgcc
     2461 gttgaagttg ccaccgatcg gaatcaccga gaacacgatg aacgccggca ccaacgacag
     2521 cagcggcgcg tacttgaaca ccgcccgatc ggcctgatcg gggatcaggt cgctcttgaa
     2581 catgatcttc atgccgtcgg ccagcgtctg caggacaccg aacgggccgg cccaacgcgg
     2641 accgaggcgg ttcgagaagt cggcgtgcaa cttgcgttcg aaccagatca tgaggatcac
     2701 cgacgtcatc aaaatgccga aggcgacgag caccttcgcc aacatgacga gcacaccggt
     2761 gagatcgagg ttgttgagga gcggatcgag agcgatcatc gcgccgcctc cagacggatc
     2821 tcggtgatcg gggcgccggc ctcgatcagg tcgttcacgt tgacgttcgg ggcgttcacc
     2881 gcaagggcgg cggtgccctg ggccacggtc gcgtccgcg
//
LOCUS       DAOOMA010000337         2912 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_41687_length_2912_cov_3.492825, whole genome shotgun sequence.
ACCESSION   DAOOMA010000337 DAOOMA010000000
VERSION     DAOOMA010000337.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 2912)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2912)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2912
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41687_length_2912_cov_3.492825"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..490
                     /locus_tag="PLP95_09210"
     CDS             <1..490
                     /locus_tag="PLP95_09210"
                     /inference="COORDINATES: protein motif:HMM:NF012652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HPB46017.1"
                     /translation="DRPEIDLRKLVAGFLRMAPDIAIVGEVRDKEALPLLLTLSSGVK
                     GYTTIHAGSARQALTRLRFICQLADSASQLPISALNTLVSEAIDVVVHCERGPQGPRV
                     TGVVAVEDLVAGVESAQFTTTEIYTRRTLKDRLEPTGAIPSRLAELFERAGLCITEEL
                     GS"
     gene            487..1368
                     /locus_tag="PLP95_09215"
     CDS             487..1368
                     /locus_tag="PLP95_09215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB46018.1"
                     /translation="MIWALAATAGYGAYLLYTAYAFRWSGLRPAPSSGRLGPTLTERL
                     RQWMRQAGIEDVEPREFVAVTVALAAVGAVSGFLLFGGVLAPLAVGAFVGSSPVASYR
                     VRRANRRATANDAWPQLIEEIRVLTGAAGRSIPQALLEVGRRGPEELRSAFDAAHREW
                     VITTDFERTVAVLKERLADPTADATCETLLVAHQIGGGDLDRRLAELAEDRRVDNLGR
                     KDARAKQAGVRFARRFVIAVPIGMAVAGMSLGDGRASYQSASGQIMVAVGLSLVALCW
                     VWSGAMLKLPDTDRVFD"
     gene            1395..2276
                     /locus_tag="PLP95_09220"
     CDS             1395..2276
                     /locus_tag="PLP95_09220"
                     /inference="COORDINATES: protein motif:HMM:NF012694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HPB46019.1"
                     /translation="MPFAVGLSLMFSEMRWFRTPPLVERLRPYAPPGTGEQHRRGWLN
                     AASLRDVIGPLVTTFGNRVSTLLGVNDDLSVRLQRAEVHLEVTEFRLKQATYALASMV
                     AAAGIGAMASMGPSFAIAATVCAPVLTFLILEQRVTTASQRWQEAITLELPVVIEQLG
                     MLLSAGYSIGAAINRLGRRGRGRSAVELAKVSARIRQGIPEIDALREWAAYTDLAAVE
                     RLVGVLALNWEASDLGALISAEARSVRREVQRQQVELIERRSQQVWVPVTVATLVPGV
                     IFMAVPFIDAMGKLTGR"
     gene            2325..2513
                     /locus_tag="PLP95_09225"
     CDS             2325..2513
                     /locus_tag="PLP95_09225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB46020.1"
                     /translation="MTNEQPTPTKRHRRSERGEGVISTAIAVLIVAFLGVGMWAGFNR
                     IFTDAVDTTEQQVRSLGD"
     gene            2546..>2912
                     /locus_tag="PLP95_09230"
     CDS             2546..>2912
                     /locus_tag="PLP95_09230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB46021.1"
                     /translation="MSTCFGVGVFLVLLTFASHVLLNLWTLSTLDAVAHDAATDVATS
                     GTDAEHLAAVETEAIRRAREALGSWGEHAEIRVAASSIDDPVVVTVSGPSLSLLPEPV
                     AAALGLHHLERTATVQRELV"
BASE COUNT          453 a         1012 c          940 g          507 t
ORIGIN      
        1 agaccgcccc gagatcgacc tgcggaagct cgtcgccggg ttcctgcgca tggcccccga
       61 catcgccatc gtcggcgaag ttcgagacaa agaagcgctc ccgcttctgc tcacactgtc
      121 gagcggggtg aagggataca ccacgattca cgccggatcg gccagacagg cgctgaccag
      181 gctccgcttc atctgccaac tcgccgattc cgcctcacaa cttccgatct cggcgctcaa
      241 cacgctggtc tccgaagcga ttgacgtggt cgttcactgc gagcgcggcc cgcagggtcc
      301 acgggtcacc ggcgtcgtcg ccgtcgagga cctcgtcgcc ggggtcgagt ccgcacagtt
      361 caccaccacc gagatctaca cccggcggac gctgaaggac cgtctcgagc cgaccggggc
      421 gattccgtcg cgactcgctg agctcttcga gcgggccggg ctctgcatca ccgaggagtt
      481 ggggtcgtga tctgggcact ggcggcgact gccggctacg gcgcctatct gctctacacc
      541 gcctacgcgt ttcgatggag cggcctgcga cccgcaccct cgagcgggcg actcggcccg
      601 accctgaccg agcgacttcg gcagtggatg cgacaggcgg gcatcgaaga cgtcgaaccg
      661 cgcgagttcg tcgcggtgac cgtcgcactc gctgcggtcg gagccgtgag cgggttcctg
      721 ttgttcggcg gggtcctggc tcccctcgct gtcggagcgt tcgtcggctc gtcccccgtc
      781 gcctcctacc gcgtgcgacg ggcgaatcgt cgtgccaccg ccaacgatgc gtggccacag
      841 ctgatcgagg agatccgcgt cttgaccggg gccgcggggc gatcgatccc acaggcgctc
      901 ctcgaggtcg gacgtcgcgg ccccgaggaa ctgcgctccg ccttcgatgc cgcccaccgc
      961 gaatgggtca tcaccaccga tttcgaacgc accgtcgcgg tattgaagga acgtctcgcc
     1021 gaccccaccg ccgatgcgac ctgtgagacc ctgctcgtcg ctcaccagat cggcggcggc
     1081 gacctcgacc gacggctcgc ggaactcgca gaggaccgtc gggtcgacaa cctcggccgc
     1141 aaggacgccc gagccaagca ggccggtgtc cgcttcgcca ggcgcttcgt catcgcggta
     1201 ccgatcggga tggccgtcgc cgggatgtcg ctcggtgacg gacgtgcctc gtatcagtcc
     1261 gcttcgggac agatcatggt cgccgtcgga ctctcgctgg tggcgctgtg ctgggtgtgg
     1321 tccggcgcga tgctcaaatt gcccgacacc gaccgggtct tcgactgatg ccggcctggt
     1381 acccactcct ggcattgccg ttcgcggtcg ggctttcgtt gatgttcagc gagatgcgtt
     1441 ggttccgcac gccgccactt gtcgagcggc tgcgtcccta cgctccgccg ggaaccggtg
     1501 aacagcatcg acgaggatgg ctcaacgcgg catcgcttcg cgacgtcatc ggcccgctcg
     1561 tcaccacgtt cggaaaccgg gtctcgaccc tgctcggggt caacgacgac ctgtcggttc
     1621 gtctgcaacg cgccgaagtc cacctcgagg tcaccgagtt ccggctcaag caggccacct
     1681 acgcgctggc gtcgatggtg gccgccgccg gtatcggcgc tatggcatcg atgggtccgt
     1741 cattcgcgat cgccgcgacg gtgtgtgcgc cggtcctgac ctttctcatc ctggaacagc
     1801 gggtcaccac cgcgtcgcag cgctggcagg aggcgatcac cctcgagctt ccggtcgtca
     1861 tcgaacaact cggcatgttg ttgtcggcgg gctactcgat cggggcggcc atcaatcgcc
     1921 tcgggcgtcg cggccgtggc cgcagcgccg tcgaactcgc caaggtcagc gcccgtatcc
     1981 gccagggcat ccccgagatc gacgcacttc gcgagtgggc cgcctacacc gatctcgcag
     2041 ccgtcgagcg cctcgtcggc gtcctggcgc tcaattggga ggcaagcgat ctcggcgcgc
     2101 tgatctccgc cgaggcgagg tcggttcgcc gcgaggttca acgtcaacag gtcgaactca
     2161 tcgagcggcg gtcccagcag gtctgggtgc ccgtcaccgt ggcgaccctc gttcccggag
     2221 tgatcttcat ggccgtcccg ttcatcgacg ccatgggaaa gctgaccggg cgatgaccgc
     2281 aacaacaacc ggtgccaagc accactcctg gagggagaac caccatgacc aacgaacaac
     2341 ccaccccgac caagcgtcac cgtcgaagcg aacggggcga gggtgttatc tccaccgcta
     2401 tagctgtctt gatcgttgcc ttcctcggtg tcggaatgtg ggccgggttc aaccggattt
     2461 tcaccgacgc cgtcgacacg accgaacagc aggtgcgctc gctcggcgac tgaacacgac
     2521 ccgcgacgat cgcgggtcgg ggacgttgag tacgtgtttc ggggtcggcg tgttcctcgt
     2581 gttgttgacc ttcgcgagcc atgtgttgtt gaacctgtgg accctcagca ccctcgacgc
     2641 ggtcgcccac gacgcggcga ccgacgtggc cacctccggc accgatgccg aacacctggc
     2701 cgcggtcgag accgaggcga ttcgacgggc gcgcgaagca ctcggttcgt ggggcgaaca
     2761 cgccgagatc cgtgtcgcgg catcttcgat cgacgatccc gtcgtcgtca ccgtgtcggg
     2821 cccgtcgctc agcctgttgc ccgaaccggt cgcggcggcg ttggggctcc atcacctcga
     2881 gcgcactgcc accgtgcaac gcgaactggt gg
//
LOCUS       DAOOMA010000338         2911 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_41706_length_2911_cov_3.862745, whole genome shotgun sequence.
ACCESSION   DAOOMA010000338 DAOOMA010000000
VERSION     DAOOMA010000338.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 2911)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2911)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2911
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41706_length_2911_cov_3.862745"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1758)
                     /locus_tag="PLP95_09235"
     CDS             complement(<1..1758)
                     /locus_tag="PLP95_09235"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF024642.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HPB46022.1"
                     /translation="MDLEVPTLPDDADGNDPTLLTATRDRLLGGLNPNQRAAVLSDAA
                     VLRIIAGAGSGKTRVLTRRIARRVADTEVDPRRVLAVTFTRKAAAELRNRIDRLGLRG
                     GINAGTFHSIAYSQLRSRWEERGIAPPALLDRKFGLVASLLPRDHARSATLPLDVISE
                     IEWAKARMIPASDYAAQATAATRRLPIDATAIADLYQRYEQEKLARRMVDFDDILRLA
                     TRDLEADAEYAAARRWRFRHLFVDEFQDVNPLQFRLLQAWVGGVPDLCVVGDPNQAIY
                     AWNGADSRYLDDFETLFPGAQTIDLSDNYRSTPQILGLANTVLTLAPSRRFDLQPNRP
                     DGPVPTIVSYDSETAEAQGVARAARDAHRPGDRWGDQAVLVRTNAQLAVFEEAFSKAR
                     IPFRSRGKGRFLDQPEIKSALSRLKRSGGQLSTQLSDLDQALATGADLDGERLGDDRL
                     ANVAELVRLGREYLDLEPNGDVAGFGSFLRTSLRSDDGTGGTDAVELVTFHAAKGLEW
                     PTVYVCGLEKGLVPIHYAETSESVAEEIRLFYVAVTRAERHLRLSWCEKRSFGSRVQS
                     RERSPLLDTVELAIELLREN"
     gene            1802..2485
                     /locus_tag="PLP95_09240"
     CDS             1802..2485
                     /locus_tag="PLP95_09240"
                     /inference="COORDINATES: protein motif:HMM:NF015648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HPB46023.1"
                     /translation="MAFPRSHLHENEQIVIDRSPHWWYLTPRALLLVVASALGIWALV
                     YDFEDGSRWQQPVRVIVGLLVAAAFLNFLVRFIQWRATHFVITTDRCIYRSGVLKKSG
                     IEIPLERINTVLFSQTVFERILGAGDIAIESAGEGSRQNFSDVSNPVQVQNMIYRQIE
                     ENENRKYDRIGVEAREAAAASAAASGIPQPTAVLSVAEQLHKLAELRDRGVITDAQFE
                     TQKRTLLGE"
     gene            complement(2521..>2911)
                     /locus_tag="PLP95_09245"
     CDS             complement(2521..>2911)
                     /locus_tag="PLP95_09245"
                     /inference="COORDINATES: protein motif:HMM:NF020133.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB46024.1"
                     /translation="DDLAPLAERADHDHDHDHDTLLRRARHVVTENARVDEVIDAFGR
                     GDLEGAGDALWRGHRSLAEDFDTSTPLVDDLIAHLRATDGVYGARMTGAGFGGCVVAL
                     CRAGLDPTPDGLASWEVRPTDGVRLLD"
BASE COUNT          473 a          916 c         1032 g          490 t
ORIGIN      
        1 gttctcgcgg agcagttcga tggcgagttc gacggtgtcg agcagcggtg aacgctcgcg
       61 cgactggacg cgggaaccga aggaacgctt ctcgcaccac gacagccgga ggtgacgctc
      121 ggctcgcgtg accgcgacgt agaagaggcg aatctcctcc gcgaccgact cggaggtctc
      181 ggcgtagtgg atggggacga gccctttttc gagcccgcac acgtacaccg tcggccactc
      241 aaggcccttg gccgcgtgga acgtgacgag ttcgaccgcg tcggtgccgc cggttccgtc
      301 gtcgctgcga agcgaggtgc gcaggaacga accgaacccc gccacgtcgc cgttcggttc
      361 gaggtcgaga tactcccgac cgaggcgcac tagttctgcg acgttcgcca ggcggtcgtc
      421 tccgagccgt tcaccgtcga ggtcggcgcc ggtggcaagg gcctgatcga ggtcgctgag
      481 ttgggtcgat aattgcccac ccgatcgttt gagccgcgac aacgccgatt tgatctcggg
      541 ctgatccagg aagcgcccct tgcctcggct ccggaagggg atccgcgctt tcgaaaacgc
      601 ctcctcgaac accgcgagct gggcatttgt gcgcacgagc accgcctgat ctccccagcg
      661 gtcgccgggg cggtgggcat cgcgggcggc gcgcgcgacc ccctgggcct cggcggtttc
      721 ggagtcgtag gaaacgatcg tcggcaccgg cccatcgggg cgattgggct gcaggtcgaa
      781 gcgccgcgac ggcgcgaggg tgagcacggt gttcgccaga cccaggatct ggggggtgga
      841 acggtagttg tcggaaaggt cgatggtctg ggcgcccgga aacagcgtct caaagtcatc
      901 gaggtaacga gaatcggcac cgttccatgc gtagatcgcc tggttggggt cgccgacgac
      961 acacaggtcg ggcacgccac caacccacgc ttgtaacagg cggaactgaa gtgggttgac
     1021 gtcttggaac tcatcgacga acaggtgtcg gaagcgccac cgtctcgcgg cggcgtattc
     1081 ggcgtcggct tcgaggtcgc gggtggcgag gcgcagaatg tcgtcgaagt cgaccatgcg
     1141 tcgggccaac ttctcctgtt cgtagcgctg atagagatcg gcgatcgccg tggcgtcgat
     1201 cggtaggcgg cgtgtggcag cggttgcctg cgccgcgtag tcggacgcgg gaatcattcg
     1261 ggccttcgcc cattcgatct cggagatcac gtcgaggggc agggtggccg aacgggcgtg
     1321 atctcggggc aacagcgacg ccacgagccc gaacttgcgg tcgagcagcg ctgggggagc
     1381 gatgccgcgc tcttcccatc ggctgcgcag ctgggaatac gcgatggagt ggaacgttcc
     1441 ggcgttgatg cctccccgca ggccgagccg gtcgatgcgg ttgcgcaact ccgccgcggc
     1501 cttgcgtgtg aaggtgacgg cgagaacccg gcgcggatcg acctcggtgt cggcgacgcg
     1561 ccgggcgatg cgccgggtga gcacgcgggt ctttcctgaa ccggccccgg cgatgatgcg
     1621 cagcaccgca gcgtcgctga gtaccgcggc tcgttggttc gggttgagcc cgccgagcag
     1681 ccgatcgcgc gtcgcggtga gcagtgtggg gtcgttgccg tcggcgtcgt cgggcagggt
     1741 cggtacctct agatccatcg ggtacgaccc taccgagcac cgccgacagt atcgtgggca
     1801 cgtggccttt cctcgatcgc accttcacga gaacgaacag atcgtcatcg accggtcccc
     1861 ccactggtgg tatctgacac cgagggcgct gttgttggtc gtcgcctctg cgctcgggat
     1921 ctgggcactg gtctacgact tcgaggacgg atcgcgctgg caacaacccg tgcgtgtgat
     1981 cgtcgggttg ttggtggcgg cggcgttcct gaacttcctc gttcggttca ttcagtggcg
     2041 cgccacccac ttcgtcatca cgaccgatcg ctgcatctat cgatctggcg tgctcaagaa
     2101 gtcgggaatc gagatcccac tcgagcgcat caacaccgtc ttgttctccc agaccgtgtt
     2161 cgaacggatc ctcggagccg gcgatatcgc catcgagtcc gcaggtgagg gttcgcgcca
     2221 gaacttctcc gacgtgagca atccggttca ggtgcagaac atgatctatc gacagatcga
     2281 ggagaacgag aaccgcaagt acgaccgcat cggggtcgag gcgcgcgagg cggcagcggc
     2341 ctcggcagcc gcctcgggga tccctcagcc gacggcggtg ctgagcgtcg ccgaacagct
     2401 ccacaagctg gccgagctgc gcgatcgcgg cgtgatcacc gacgcgcagt tcgaaacgca
     2461 gaagcggacc ctgctcggcg agtagccgct cggccgggac cggctcagtc cgggaccggc
     2521 tcagtccagg agccgcaccc cgtcggtagg tcgaacctcc cagctcgcca ggccatcggg
     2581 ggtcgggtcg agtccggcgc ggcacagcgc aaccacgcaa ccgccgaagc cagcgccggt
     2641 catccgcgca ccgtacacgc cgtcggtggc gcgcaggtgg gcgatgaggt cgtcgacgag
     2701 gggggtcgag gtgtcgaagt cctcggccag gctgcggtgg ccgcgccaca aggcgtcgcc
     2761 ggcgccctcg aggtcgccgc gtccgaaggc gtcgatgacc tcgtcgaccc gtgcgttctc
     2821 ggtcacgaca tggcgggcac ggcgcagcaa cgtgtcgtgg tcgtggtcgt ggtcgtggtc
     2881 cgcgcgttcg gcgagcgggg cgagatcgtc g
//
LOCUS       DAOOMA010000339         2906 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_41796_length_2906_cov_5.721150, whole genome shotgun sequence.
ACCESSION   DAOOMA010000339 DAOOMA010000000
VERSION     DAOOMA010000339.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 2906)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2906)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2906
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41796_length_2906_cov_5.721150"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(208..1194)
                     /locus_tag="PLP95_09250"
     CDS             complement(208..1194)
                     /locus_tag="PLP95_09250"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HPB46025.1"
                     /translation="MVSRSALGLGGIGLAALSSVAIVGHRVRRRHNPELDALAHEPTS
                     IVHHHLRTADGGVVHVAEAGTGRPVVLLHGVTLQWWVWSPVMNLLSDRYRVIAWDMPG
                     HGESELGSDGFTMETLCRDLAMVLDELDVRDAVLVGHSLGGCTVGGFLSRQPETLAER
                     VGALMFVATAATLAEGLATKGSPRTLFGLPGGALLSGLRKPRPSNPWTDNDLNAGLVA
                     MAFGPGATARMVSSVRRMIAEFPASTSVAAAPVLLRHDATAALSSAAIPTTVLAGQYD
                     RLLPPGHSRQIANLVRGSRLVELDGVGHQIMQEAPHELVAALDDLVTRASTH"
     gene            complement(1236..1514)
                     /locus_tag="PLP95_09255"
     CDS             complement(1236..1514)
                     /locus_tag="PLP95_09255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB46026.1"
                     /translation="MGVQRTSFGKLQRDRAKKAKQEAKRERRAANAAAAEEERDIETI
                     LGDEDKELSAEELLEAIEKIHRQFENDEIDFEEFEELKLELFARLPVD"
     gene            complement(1619..1819)
                     /locus_tag="PLP95_09260"
     CDS             complement(1619..1819)
                     /locus_tag="PLP95_09260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_465540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HPB46027.1"
                     /translation="MATGTVKFFNQEKGFGFISRENADDIFVHYSNIQGSGFKTLEPG
                     QEVEFDTAPGRKGEEAQNVRVL"
     gene            complement(1915..>2906)
                     /locus_tag="PLP95_09265"
     CDS             complement(1915..>2906)
                     /locus_tag="PLP95_09265"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HPB46028.1"
                     /translation="EGAAELRDVLGERLGVSGGEVIVTTGSQQGIDLVSRCLLEAGDV
                     VVVESPTYLGSLQAFRAQEARVEAIEGDRWGLDIGRLADALSAGLRPRLVSVVSEFAN
                     PSGATLSFERRRALVDLARRYRFVILDDNPYGELRWSGTSPVGLEEIAATSADDGRND
                     CVVSLGSASKILAPGLRVGWLRAPEEIRRSVVTLKQATDLQTSSLDQRLVAELLGDAD
                     WMDAHLATARETYQRRCSVLLDAVLGGFGDAVDVDQPDGGMFLWVRFNRSKIDTMELF
                     RRSIELGVAFVPGEAFGSRSGRSHAMRLCFATLKSEELRTAVRRLVRAAQPML"
BASE COUNT          524 a         1009 c          883 g          490 t
ORIGIN      
        1 tgtcaccgtt gttggtgtgt tgggatgccc tgtcgtgctc gaacatgtgt tcatggtatc
       61 gaacgacagt tcggcttgca agccgaaagc ccacgaatcc agaacaaatg ttcggcacct
      121 ccgcgcgcca ccaacacgcc gccgcgcaca cctcggagcg cgctcacgcc aacgcgacgc
      181 agccacccac aacccatccg ggctcggtca gtgcgtcgaa gctcgtgtca cgagatcatc
      241 cagcgcggcg acgagctcat gcggcgcctc ctgcatgatc tgatgcccga cgccgtcgag
      301 ctcgacgagt cgtgagccgc gcaccaggtt cgcgatctgc cggctgtggc ccggaggcag
      361 gagtcggtcg tactgtccgg ccagcaccgt cgtcggaatc gccgccgacg acagcgccgc
      421 ggtggcgtcg tgacgcaaca gcaccggcgc tgcggcgacg ctcgtcgacg ctggaaactc
      481 ggcgatcatc cggcgaaccg acgagaccat ccgagcggtg gccccgggcc cgaacgccat
      541 ggcgaccaac cctgcgttga ggtcgttgtc ggtccaggga ttcgacgggc ggggcttgcg
      601 caggccgctg agcagcgcac cgccgggtag cccgaacagc gttctggggc tgcccttggt
      661 cgcgaggccc tccgccagcg tcgctgcggt ggcgacgaac atgagcgccc cgacgcgctc
      721 cgccagcgtc tccggctgcc gcgagagaaa cccgccgacg gtacacccgc cgagggaatg
      781 cccgacgagc acggcgtcgc ggacgtcgag ttcgtcgagg accatcgcca gatctcgaca
      841 caacgtctcc atcgtgaacc cgtcggatcc gagttccgac tcgccatgcc cgggcatgtc
      901 ccaggcgatg acgcgatagc ggtcgctcaa caggttcatg accggcgacc acacccacca
      961 ttgcagcgtc acgccgtgaa gcaacaccac cggtcggccg gtgccagctt cggcgacgtg
     1021 cacgacgccg ccatcagcgg tgcgaaggtg atggtgaaca atcgaggtgg gctcgtgcgc
     1081 cagagcatcg agttcggggt tgtgacgccg acgtacccga tgcccgacga tcgctaccga
     1141 tgacagcgcc gccagaccga tcccgcccag tccgagtgcg ctacgggata ccaaacgaac
     1201 gactcgcgat gcgaacccca cgatcgtggc gccgttcagt cgacggggag acgggcgaag
     1261 agttcgagct tcagttcctc gaattcctcg aaatcgatct cgtcgttttc aaactggcga
     1321 tggatcttct cgatcgcctc gaggagctcc tcggccgaga gctccttgtc ctcatcaccg
     1381 aggatggttt cgatgtctcg ctcttcttca gcggccgccg cgttcgctgc gcggcgctcc
     1441 cgcttggctt cctgcttcgc cttcttggct cgatctcgct gcagtttgcc gaaacttgtt
     1501 cgttgtacgc ccatgatggg gaccttcttg ttctcgaacg ggtcgatcgg atcgacccgc
     1561 tatgcatggc aatgccgccg acttgcgtcg gcggcatctg cacacggctc cgtgactctt
     1621 aaagaacgcg aacgttctga gcttcttcgc ccttgcggcc gggagcggtg tcgaattcga
     1681 cctcctggcc tggctcgagg gtcttaaaac cagacccctg gatgttggag tagtggacga
     1741 agatgtcgtc cgcgttttca cgggagataa aaccgaagcc cttctcttgg ttgaagaact
     1801 tcaccgtgcc agttgccaac gtattctctt ttcgtaatgc gacccccgat gcgagggctt
     1861 cagacagtgt aggcgcacga ggcccgtcgc gcccctcacc tgagcggtcc gtcgtcacaa
     1921 catcggttgg gccgccctca ccagccgccg aaccgccgtg cggagttcct cggatttcaa
     1981 cgtcgcgaag cacagccgca tggcgtggga acggccactg cggctgccga acgcctcccc
     2041 cggcacgaac gcaaccccca gttcgatcga tcggcgaaac agttccatcg tgtcgatctt
     2101 tgaccggttg aacctcaccc acaggaacat cccaccgtcg ggttgatcga catcgactgc
     2161 atcgccgaag ccgccaagaa ccgcatcgag cagcaccgaa cagcggcgtt ggtaggtctc
     2221 ccgcgccgtg gccaggtgcg cgtccatcca gtcggcgtcg cccagcaact cggccacgag
     2281 gcgttggtcg agcgaactcg tctggaggtc ggtcgcctgc ttcagcgtca ccaccgagcg
     2341 ccgaatctcc tcgggggcgc gcaaccaccc gacccgaaga cccggagcga gaatcttgga
     2401 ggccgatccc agcgacacga cgcaatcgtt tcgtccgtca tcggccgagg tcgcggcgat
     2461 ctcttcgagc ccgacaggac tcgtcccgct ccaacgcaat tcgccgtagg ggttgtcgtc
     2521 gaggatgacg aagcggtagc gacgcgctag gtcgaccagc gcgcgccggc gttcgaaact
     2581 caacgtcgca ccggacggat tggcgaactc ggacacgacc gacacgagcc gtgggcgcag
     2641 accggcgctc agagcatcgg ccagccgacc gatgtcgagg ccccagcggt cgccctcgat
     2701 ggcctcgacg cgtgcctcct gcgcgcgaaa cgcctgcaac gaacccaggt aggtcggcga
     2761 ctcgaccacc acaacgtcgc cggcttccag gaggcaacgc gacaccaggt cgatcccctg
     2821 ttgcgagccc gtggtcacga tcacctcgcc tccgctcacc ccaagccgct cccccaacac
     2881 atcgcgaagc tcggccgcgc cctcgg
//
LOCUS       DAOOMA010000340         2903 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_41848_length_2903_cov_3.229986, whole genome shotgun sequence.
ACCESSION   DAOOMA010000340 DAOOMA010000000
VERSION     DAOOMA010000340.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 2903)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2903)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2903
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41848_length_2903_cov_3.229986"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            95..1219
                     /locus_tag="PLP95_09270"
     CDS             95..1219
                     /locus_tag="PLP95_09270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxide hydrolase"
                     /protein_id="HPB46029.1"
                     /translation="MSFRVEISDEEIADLRRRLEHTRWPDPEPVDDWSQGVPLDYVRD
                     LCQWWARDYDMGLADRVNAFDQVKVDIDGLGFHAIHARSAEPDALPLVLTHGWPGSVV
                     EFLDVIGPLTDPVAHGGDAGDAFHVVAPSLPGYGWSDKPTETGWGLDRIAAAWRALMG
                     VFGYERYVAQGGDWGSGVTSTLGALGEGGPVAIHLNMPFAFPDPDTMDDLTPTETAAL
                     ADMMEHAESGMGYSSQQSTRPQTLGYGLADSPAGQLAWIVEKFWAWTDHAGTPDSALS
                     RTQMLDDVSAYWFTDTAASSARLYWQDFKKLQAPSAISVPVGVTVFPREIIRPSRRWA
                     EKAYPTLAWFEEVEAGGHFAALEQPEIFVDQLRRYFRQFR"
     gene            complement(1294..1677)
                     /locus_tag="PLP95_09275"
     CDS             complement(1294..1677)
                     /locus_tag="PLP95_09275"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004847071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004068 - aspartate 1-decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006523 - alanine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HPB46030.1"
                     /translation="MRRRMLNAKIHRATVTDANLDYVGSITIDRDLMDAADLLEYEQV
                     AVVDVTNGARLETYVIEGPRGSGVICVNGAAAHLVHRDDLVIIISYVDVEQAELASHV
                     PTVVHVDADNRIVSEDIAANAAAAS"
     gene            complement(1901..2521)
                     /locus_tag="PLP95_09280"
     CDS             complement(1901..2521)
                     /locus_tag="PLP95_09280"
                     /inference="COORDINATES: protein motif:HMM:NF013053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HPB46031.1"
                     /translation="MARRPLNELVAPGHTAIVTQECQGAVIGPNAGLAELAAEARREA
                     IPNLVRLLDAARCVDATVVHCLAERRADGRGSNHNARLFAAAAGLGLQLERGTEGASL
                     LPELGPEPSDLVLTRWHGVGPMAGTDLDIVLRNLGVRTIIVTGVSVNVAITNFVMDAV
                     NLGYDVVLPRDAVAGVPTDYANAVIDNTLSLLATVVTTDDLLAAWE"
     gene            complement(2612..>2903)
                     /locus_tag="PLP95_09285"
     CDS             complement(2612..>2903)
                     /locus_tag="PLP95_09285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB46032.1"
                     /translation="VDDLAAGIGRVRELRTLGGGDAPGDAFDICWSEALLSDLAASRD
                     HQCSRLSALEAVGVTWSSVALGGENRAELIDTIERFGREVIAEFPATEFPAT"
BASE COUNT          445 a          881 c         1039 g          538 t
ORIGIN      
        1 gggcaccaag gggttctcga gggagggtga agtgtcggag agtgaagtgt cggagggtga
       61 tgtgacaggg catgggttgg cggtcggtga gccggtgtcg tttcgggtcg agatctccga
      121 tgaggagatt gcggatcttc gccgtcgtct cgaacacact cgttggcccg accccgaacc
      181 ggtcgatgac tggagccagg gcgtgccgct cgactacgtc cgtgacctgt gtcagtggtg
      241 ggcccgggat tacgacatgg gcctcgctga tcgggtgaac gcattcgatc aggtgaaggt
      301 cgacatcgac ggtctcggct ttcacgccat tcatgcgcgt tctgccgaac cggatgctct
      361 gccgttggtc ctcacgcatg gctggccggg aagcgtggtg gagttcctcg acgtcatcgg
      421 cccgctcacc gatcccgtcg cccacggtgg agacgctggc gatgcgttcc acgtggtcgc
      481 gccgtcgctg cccgggtacg ggtggagcga caagccgacc gaaaccgggt ggggactcga
      541 ccgaatcgcc gcggcgtggc gggcgctgat gggcgtgttt ggttacgagc gttatgtcgc
      601 ccagggcggt gactggggtt cgggggtgac ctcgacgttg ggggcgctgg gggagggcgg
      661 cccggtcgcc atccatctca acatgccgtt cgctttcccc gatccggaca cgatggacga
      721 tctgaccccg acggagaccg ccgcgctcgc cgacatgatg gagcatgccg agtcggggat
      781 ggggtactcg tcgcaacagt cgactcggcc tcagacactc gggtacgggc tcgcggacag
      841 cccggcgggc cagctggcgt ggatcgtcga gaagttctgg gcgtggaccg accacgccgg
      901 cacccccgac tcggcgttga gtcgaaccca gatgctcgac gatgtgagcg cctactggtt
      961 caccgacacc gcggcgtcat ctgcccgcct gtattggcag gatttcaaga agctccaggc
     1021 gccgtcggcc atctcggtgc cggtgggcgt gacggtgttt cctcgggaga tcatccggcc
     1081 gtcgcggcga tgggccgaga aggcctatcc gacgttggca tggttcgagg aggtcgaggc
     1141 cggcgggcac ttcgcggcgc tcgaacagcc ggagattttc gtcgaccaat tacgtcgcta
     1201 tttccgccag ttccgctgat tgtgctggcc cattgcccgt tctccgataa ccagtggtcg
     1261 tgatcacgac cactggttat cggagaacga gcgtcaggag gccgcggcgg cgttcgctgc
     1321 gatgtcctcg gacacgatgc ggttgtcggc gtcgacgtgc acgacggttg gcacatggct
     1381 ggcgagttcg gcctgttcga catccacata ggagatgatg atcacgaggt cgtcgcggtg
     1441 cacgaggtgc gcggccgctc cgttcacaca gatgacaccc gagccccggg ggccctcgat
     1501 gacataggtc tcgagtcggg cgccgttggt cacgtccacc accgcgacct gttcgtattc
     1561 gaggaggtct gcggcgtcca tgaggtcgcg gtcgatggtg atcgagccga cgtagtccag
     1621 gttcgcgtcg gtcaccgtcg cccgatggat cttcgcattc agcattcgac gtctcatctc
     1681 gggctcctgt tcggggtcgc gcgttcgatt gaacacccca aacggatgtc tcgtcgcgcg
     1741 ctgcggcgaa acggtcgaat gaggtgggag atgtcgtaca accaggcgct caggcccgtg
     1801 tcgtcgaccc ggtcatcgta gcgggggatc gtagcggggg atcggcatcg gcatcggtcc
     1861 cgatcgccag cctcaggcga ccttcaggct caggcgagct tcactcccaa gcggcgagca
     1921 ggtcgtcggt ggtgacgacg gtggcgagca gcgacagcgt gttgtcgatg acggcgttcg
     1981 catagtcggt cggcactccg gccacggcgt cgcggggcag aacgacgtcg taaccgaggt
     2041 tcaccgcatc catcacgaag ttggtgatcg cgacgttcac cgacacgccg gtcacgatga
     2101 tggtgcgcac cccgaggttg cgcaggacga tgtcgaggtc ggtgccggcc atcgggccga
     2161 ccccgtgcca tcgggtgagc actagatcgc tcggctccgg cccgagttcg ggcaggagcg
     2221 aggcgccttc ggtgccgcgt tcgagttgga gcccgagtcc ggcggcggcg gcaaagagcc
     2281 gtgcgttgtg gttcgatcct cgtccgtcgg cgcggcgctc ggcgaggcag tgcacgacgg
     2341 tggcgtccac gcagcgtgca gcatcgagca gacgaacgag gttcgggatc gcttcgcgcc
     2401 gggcctcggc cgcgagttcg gcgagtccgg cgttgggtcc gatgaccgca ccctggcatt
     2461 cctgggtgac gatggcggtg tggccgggtg cgacgagttc attgagtggg cggcgtgcca
     2521 tgttgcttcc ttcgggtgct gacccccggc tcccggctcg ttctccgata accagtggtc
     2581 gtgatcacga ccactggtta tcggagaacg gtcaggtggc ggggaattcg gtggcgggga
     2641 attcggcgat gacctcgcgg ccgaatcgtt cgatggtgtc gatgagttcg gcccggttct
     2701 cgccgccgag cgccacgctc gaccaggtca cgccgaccgc ttcgagggcg ctcagccggc
     2761 tgcactggtg atcgcgtgac gcggcgaggt cgctgagcag cgcctcggac cagcagatgt
     2821 cgaacgcatc acctggtgca tcgccgccgc cgagagtgcg cagttcccgc acccgcccga
     2881 tacccgccgc gaggtcgtcg acc
//
LOCUS       DAOOMA010000341         2900 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_41906_length_2900_cov_3.841125, whole genome shotgun sequence.
ACCESSION   DAOOMA010000341 DAOOMA010000000
VERSION     DAOOMA010000341.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 2900)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2900)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2900
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41906_length_2900_cov_3.841125"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            185..952
                     /gene="rfbF"
                     /locus_tag="PLP95_09290"
     CDS             185..952
                     /gene="rfbF"
                     /locus_tag="PLP95_09290"
                     /EC_number="2.7.7.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018040698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047343 - glucose-1-phosphate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate cytidylyltransferase"
                     /protein_id="HPB46033.1"
                     /translation="MKVVILAGGVGSRLSEETDVRPKPMVEIGHRPILWHIMKHYSSY
                     GFNDFVICLGYKGEYIKRYFSDSLALASDMTIDFGEGTVEAHETVRDDWRVTLVDTGQ
                     HTETAGRISQIKKYVDDGPFLMTYGDGVSDVDLEALVKFHEAHGNLATVTAVHPTARF
                     GALAIEGDQVVRFDEKPQTEEGWINGGFFVLEPEIFDYIPGDVDWAREPMERLAAAGE
                     LNAYRHDGFWQCMDTLRDKIYLQGLWDKGGAPWKIWS"
     gene            1003..2025
                     /locus_tag="PLP95_09295"
     CDS             1003..2025
                     /locus_tag="PLP95_09295"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HPB46034.1"
                     /translation="MKVLLTGAEGYIGVRMGHYLLERGHDVTGIDSGFHRVGWLYNTD
                     ERRPAMLTKDTRDIQLDDLRGYEAVVHLAEVSNDPVGELNGDVTYKINHEGSLRLAKL
                     AKEAGVERFVHMSSCSVYGASGERPSKEGDPVGGLTAYAKCKVLIEQEVSPLADDNFS
                     PTFMRNATAYGASPRQRFDLVVNDLAASAYLYKEIRMASDGTPWRPFVHILDISHAVA
                     CTLEAPRDVIHNEIFNVGNDRSNYQVRQIAEIIGSLIPGCELIFGDSSADKRNYRADF
                     TKIHEQLPGFECAWDVERGAKELIEIFDRIGFDEEMYRFRGHTRIKQIKHLIDTGQID
                     DDFFWR"
     gene            2036..2584
                     /locus_tag="PLP95_09300"
     CDS             2036..2584
                     /locus_tag="PLP95_09300"
                     /inference="COORDINATES: protein motif:HMM:NF013103.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase family
                     protein"
                     /protein_id="HPB46035.1"
                     /translation="MKFTELGVAGAFYIELEPNVDDRGSFSRAWCAQELREHGAVGEI
                     AQMNLSTNHRRGTIRGLHVQNPPHGEAKFFRCLAAASYHVIADVRPDSPTYGAWAGVE
                     LSAERFDALYVPPYCAKGYQALADDTAVLYAVSSPYTPGAETGLRYDDPAFGITWPIT
                     DGVIVSDKDRAWPDVQLERSKS"
     gene            2581..>2900
                     /locus_tag="PLP95_09305"
     CDS             2581..>2900
                     /locus_tag="PLP95_09305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB46036.1"
                     /translation="MIIVDTALHKRQEAGDPVRVGLIGSGFAGQGFVDQVMDHTPGMT
                     ISVVANRTLAGAAQAFSQIGIHDVDVVSTQAELDSSIAARRPAITDDPSLVYENGQVE
                     AVVEA"
BASE COUNT          586 a          882 c          913 g          519 t
ORIGIN      
        1 gaagtgggag cgagcggcgt cggaactgga atcgttggac ccggcacgat gagttcgtcg
       61 acgtccgggg aggtatcggt gatgtgggcg acagcaaacg ggccgaaatc ctcattgaac
      121 caccgggtcg atttgggcac gttctgtgcg tcaagtgtcc acagcgcagc atgaggagcg
      181 gcggatgaag gtagtcatac ttgctggtgg tgtcgggagc cggctcagcg aagagaccga
      241 cgtgcgaccc aagccgatgg tcgagatcgg acatcgaccg atcctgtggc acatcatgaa
      301 gcactacagc tcgtacgggt tcaacgactt cgtgatctgt ctgggctaca agggcgagta
      361 catcaagcgg tacttctccg attcgttggc gttggcctcg gacatgacca tcgacttcgg
      421 ggaaggaacg gtcgaggccc acgagaccgt ccgtgacgac tggcgtgtca ccctcgtcga
      481 cacgggccag cacaccgaaa ccgcagggcg gatctcccag atcaagaagt acgtcgacga
      541 cggaccgttc ctcatgacct acggcgacgg ggtctccgac gtcgacctcg aggccctcgt
      601 caagttccac gaggcccacg gcaacctcgc gacagttacc gccgtgcatc cgaccgcccg
      661 cttcggtgcg ctggcgatcg agggcgacca ggtggtccgc ttcgacgaga agccccaaac
      721 cgaggaggga tggatcaacg gtggattctt cgtgctcgag cccgagatct tcgactacat
      781 ccccggagat gtcgactggg cccgcgagcc gatggaacga cttgcggccg ccggagaatt
      841 gaatgcgtac cgccacgacg gtttttggca gtgcatggat acccttcgag acaagatcta
      901 tttgcagggt ctttgggaca agggcggagc gccctggaaa atctggagct gagcggttcg
      961 aacattcgcg acgaccgctc gatcgaaagg caagacaaac gaatgaaggt tctcctgacc
     1021 ggagccgagg gttatatcgg cgtgcgtatg ggtcactatc tgcttgagcg tggccacgac
     1081 gtcacgggta tcgacagcgg attccatcgg gtggggtggc tctacaacac cgatgagcgg
     1141 cgtcccgcca tgctcaccaa ggacacccgc gacatccaac tcgacgacct gcgtggttac
     1201 gaggccgtgg tgcacctcgc cgaggtgtca aacgatccgg tcggcgagct gaacggcgac
     1261 gtcacctaca agatcaacca tgagggcagc ctgcgccttg ccaaattggc gaaggaagcc
     1321 ggtgttgagc gcttcgtcca catgagttcg tgttcggtgt acggcgcttc tggcgaacgg
     1381 ccctcgaagg agggcgaccc ggtcggtggg ctcacggctt atgccaagtg caaggtcctc
     1441 atcgaacagg aggtgtcgcc actcgctgat gacaacttct cgccgacgtt catgcgaaac
     1501 gccacagcgt atggtgcctc tccgcgccaa cgcttcgacc tcgtggtcaa cgacctcgcg
     1561 gcgagcgcgt atctctacaa agagatccgg atggcctccg acggcactcc gtggcgcccg
     1621 ttcgtgcaca tcctcgacat ctcccacgcg gtggcatgca ccctcgaggc gccccgcgac
     1681 gtgatccaca acgagatctt caacgtcggc aacgaccgtt cgaactatca ggtccgccag
     1741 atcgccgaga tcatcggaag cctcattccc gggtgcgagc tgatcttcgg tgattccagc
     1801 gccgacaagc gaaactaccg ggccgacttc acgaagatcc acgaacaact ccctggtttc
     1861 gaatgcgcgt gggacgtcga gcgtggggcc aaggaactca tcgagatctt cgaccgaatc
     1921 ggcttcgacg aagagatgta ccgattccgt ggtcacaccc gaatcaagca gatcaagcat
     1981 ctgatcgaca cgggtcagat cgacgacgac ttcttctggc gctgagctcg ctgtcatgaa
     2041 gttcaccgaa ctcggcgtcg ccggcgcctt ctacatcgaa ctcgaaccga acgtcgacga
     2101 tcgcggatcg ttctcgcgtg cctggtgcgc ccaggaactg cgcgagcacg gtgcggtggg
     2161 tgagatcgcg cagatgaatc tgtcgacgaa tcaccgtcgt ggcacgattc gcgggctgca
     2221 cgtgcagaac cctccccacg gtgaggcgaa gttcttccgc tgcctcgccg cagcgagcta
     2281 tcacgtcatc gctgatgtcc gaccggactc gccgacctac ggcgcatggg caggcgttga
     2341 gctgtcggcc gagcgattcg acgcgctgta tgtgccgccg tattgcgcga agggctatca
     2401 ggcactggcg gacgacaccg ctgtcctcta cgccgtgtcg agtccctaca ccccaggggc
     2461 ggagacggga cttcgctacg acgatcccgc gttcgggatc acatggccga tcaccgatgg
     2521 cgtgatcgtg tcggacaagg accgggcctg gcccgatgta cagctcgaac ggagtaaatc
     2581 atgatcatcg tcgacaccgc actgcacaag cgccaggagg cgggggaccc cgtccgggtg
     2641 gggctcatcg gatccgggtt cgccggccag gggttcgtcg accaggtgat ggatcacacc
     2701 cctggtatga cgatctcggt ggtggcgaac cgcacgctcg caggagctgc acaggcgttc
     2761 agccagatcg gcattcacga cgttgacgtc gtgtcgacac aggccgaact cgacagctcg
     2821 atcgccgctc gtcgaccggc gatcaccgac gatccgtcgc tcgtgtacga gaacggtcag
     2881 gttgaggcgg tcgtcgaggc
//
LOCUS       DAOOMA010000342         2889 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_42137_length_2889_cov_3.345448, whole genome shotgun sequence.
ACCESSION   DAOOMA010000342 DAOOMA010000000
VERSION     DAOOMA010000342.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 2889)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2889)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2889
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42137_length_2889_cov_3.345448"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1401)
                     /locus_tag="PLP95_09310"
     CDS             complement(<1..1401)
                     /locus_tag="PLP95_09310"
                     /inference="COORDINATES: protein motif:HMM:NF016143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HPB46037.1"
                     /translation="MPPSGQPSKAKKFVAAFVALALIAGVAVLAVAFGDGKGPTAGGI
                     VRVGADVSKSEPGESLRDDADTDGVELIGANGGEVDRIAAAAIADLEQYWSEQMPELF
                     DKPYDPVTGGFYSWSDGEDLPACAVSPEEIRGNAFYCGAADEIGWDDTSLMPDLLHYY
                     GDLAVAVVFAHEWGHVVQARVAMSGRTVTLEQQADCYAGAWVANARAGRSAYFSPSDS
                     DMDQALAGYLELGDARGSTPDDETAHGSAFDRINSFQEGLEDGPESCTRYNDRNVADR
                     LVQIGFYDPEDYRNDGDAPYEDILSMIDEDLNDFWGTVGPEVGSGSWTPMAAGTPFSP
                     NSGEVGSCGGDEVADTSLFYCPDDRSVHFDNDELFPEVYESFGDFGLAQLYAMHFTYG
                     ALDTYSEMPDSDEGAQRAAHCLTGAWAASIFRHDRSTSKMRLSSGDFDEAVQVLLAMS
                     AGDSAGSGFQRVSAFRL"
     gene            1870..2172
                     /locus_tag="PLP95_09315"
     CDS             1870..2172
                     /locus_tag="PLP95_09315"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative quinol monooxygenase"
                     /protein_id="HPB46038.1"
                     /translation="MLVVSGTITVGEATLDKALDAIATLVAATVNEDGCGSYGFYQTP
                     GEPTVFRVFEEWESAEAMNAHMASEHFLAFMGAAGEIGITGASLSQYSVSDKTTLM"
     gene            2175..2825
                     /gene="msrA"
                     /locus_tag="PLP95_09320"
     CDS             2175..2825
                     /gene="msrA"
                     /locus_tag="PLP95_09320"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020379182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HPB46039.1"
                     /translation="MFFSRPVPDMVTAEAALAGRDQPIVDPAPHFVSQRPLVAPFPDG
                     HQQIIVAMGCFWGAERIMWQTPGVWVTAVGYSGGFTPNPTYEETCSGRTGHTEAVLVD
                     FDPKTVSLEGLMQVFWESHDPTQLFRQGNDVGTQYRSAVYWTDEAQQPVVEGTRMAYA
                     QALIEAGLGPIVTELAPAGAFYYAEDYHQQYLAKNPNGYCGIGGTGVSCPAGLATD"
BASE COUNT          484 a          975 c          942 g          488 t
ORIGIN      
        1 gaggcgaaac gccgacacgc gctgaaagcc cgagcccgcc gagtcgccgg cactcatcgc
       61 caacagcacc tgaacggcct cgtcgaagtc cccgctcgac agccgcatct tcgacgtcga
      121 gcggtcatgg cggaagatgc tcgccgccca cgctccggtc aggcagtgag ctgcccgctg
      181 cgcgccctca tcgctgtcgg gcatctccga gtaggtgtcg agcgctccgt aggtgaaatg
      241 catcgcgtag agctgcgcga gcccgaagtc gccgaagctc tcgtacacct cgggaaagag
      301 ttcgtcgttg tcgaagtgca ccgaacggtc atcggggcaa tagaacagcg aggtgtcggc
      361 cacctcgtcg ccgccacagc ttccgacctc gccactgttc ggggagaacg gggtgccagc
      421 tgccatcggg gtccacgaac ccgagcccac ctccgggccc accgtgcccc agaagtcatt
      481 caggtcttcg tcgatcatcg agaggatgtc ctcatacggt gcatcgccgt cgttgcggta
      541 gtcctccggg tcatagaacc cgatctgcac cagacgatca gcaacgtttc gatcgttgta
      601 gcgggtacaa gactccggcc cgtcctcgag gccttcctgg aacgagttga ttcgatcgaa
      661 cgcgctgccg tgggccgttt cgtcatcggg ggtcgagccg cgggcgtcgc cgagttcgag
      721 gtatccggcg agcgcctggt ccatatcgga gtcgctgggg ctgaaatacg ccgacctccc
      781 ggcccgagcg ttggcgaccc atgcgcccgc gtagcagtcc gcctgttgtt cgagcgtcac
      841 cgttcttccc gacatcgcta cccgcgcctg gaccacatgg ccccattcgt gggcgaacac
      901 caccgcaacc gccaggtcgc cgtagtagtg caacaggtcc ggcatcaacg aggtgtcgtc
      961 ccaaccgatc tcgtccgccg caccgcagta gaacgcgttc ccgcggatct cctcgggcga
     1021 aaccgcgcac gccggcagat cctccccgtc ggaccaggag tagaacccac cggtgacggg
     1081 gtcatacggc ttatcgaaca actcgggcat ctgctcggac cagtactgct cgagatcggc
     1141 gatcgctgcg gcagcgatgc ggtcgacctc cccaccgttg gccccgatga gttcgacccc
     1201 gtcggtgtcg gcatcgtcgc gcaacgattc gcccggttcg gacttcgaca cgtcggcgcc
     1261 cacccgcacg atgcccccgg cggtggggcc tttgccatca ccgaatgcga cggccaacac
     1321 ggcgacgccg gcgatcaacg ccaacgcgac gaacgcggca acgaacttct tggccttcga
     1381 cggttgcccc gatggcggca cggcgaacgg cgactgagcg cccccgggag ggatggaccc
     1441 ggggggtccg aatccgggcg gcccgtacga ttgctgaccg tacggttgct ggccgtacgg
     1501 ttgctggccg tacccggcgg gggaatgcgt ggttggcgtc tcccatggcg cccgcggacc
     1561 gcccgccggc ggagtagctg gggtcggggc ggggtaagcg gggggtgcgg ttggcggaag
     1621 atacggtggc ggcgcgaccg gtggcggcgc ggccggcgct ggatacgccg ggggtccggt
     1681 cggaggtggc ggcggcccgg tgggcgcggc accatgagat ccgccgtctt gcgggggtcc
     1741 ccacgacggt tcgtacgggt tctccggcat cggctcaggg tagcgggcga ggccccatgg
     1801 cggatctcgg ttccgtagaa tcgtcctcag gtggatccac caccgccatc acagttgagg
     1861 ggatagatca tgctcgtcgt ttccggaacc atcaccgtcg gcgaggcgac gctcgacaag
     1921 gcgttggacg ccatcgccac gctcgtcgca gcgaccgtca atgaagacgg ttgcggaagc
     1981 tacggcttct atcagacccc cggcgagccg accgtgtttc gcgtgttcga agagtgggaa
     2041 tctgccgagg cgatgaacgc tcacatggcc tccgaacact ttcttgcgtt catgggcgcc
     2101 gctggcgaga tcggtatcac gggggcctcg ctcagccaat actccgtcag cgacaagacg
     2161 accttgatgt agccatgttc ttttctcgcc ccgtccccga catggtcacc gccgaagccg
     2221 cccttgccgg tcgcgatcaa ccgatcgtcg accccgctcc gcacttcgtg tcacaacgac
     2281 cgctcgtggc gccgtttccc gacggccacc agcagatcat cgttgcgatg gggtgcttct
     2341 ggggcgccga acgcatcatg tggcagaccc ccggcgtctg ggtcaccgcc gtcgggtact
     2401 cgggtgggtt cacccccaac ccgacctacg aggagacctg ttcggggcgc accggtcaca
     2461 ccgaggcggt gctcgtcgac ttcgatccga agaccgtgtc gctcgagggc ttgatgcagg
     2521 tgttctggga gtctcacgac cccacccagt tgtttcgcca gggcaacgac gtcggcacgc
     2581 aataccgctc ggcggtctac tggaccgacg aggcccagca gccagtcgtg gagggaaccc
     2641 gcatggcgta tgcccaggca ctcatcgagg cggggcttgg cccgatcgtg accgaactcg
     2701 cgcccgccgg agccttctat tacgccgagg attaccacca gcagtacctg gcgaagaacc
     2761 cgaacggtta ctgcggcatc ggcggcacgg gagtctcctg cccggccggc ctggccaccg
     2821 actagcccgt tctccgataa ccagtggtcg tgatcacgac cactggttat cggagaacgg
     2881 gcggggttg
//
LOCUS       DAOOMA010000343         2885 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_42214_length_2885_cov_3.406360, whole genome shotgun sequence.
ACCESSION   DAOOMA010000343 DAOOMA010000000
VERSION     DAOOMA010000343.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 2885)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2885)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2885
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42214_length_2885_cov_3.406360"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            130..1239
                     /locus_tag="PLP95_09325"
     CDS             130..1239
                     /locus_tag="PLP95_09325"
                     /inference="COORDINATES: protein motif:HMM:TIGR01254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0030975 - thiamine binding [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0048502 - ABC-type thiamine transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015888 - thiamine transport [Evidence
                     IEA];
                     GO_process: GO:0030974 - thiamine pyrophosphate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter substrate-binding
                     protein"
                     /protein_id="HPB46040.1"
                     /translation="MSRIQRRTRSGWSRRFVPVVGAMAMLATIASGCGDPDDSADRPN
                     DEGAQRPTITIVTYDSYVLDPEVEAAVEEALDVDLVISASGDGAEALAAAILTAGRPA
                     GDIFFGVDNVVMSRALSEPVFVEFDRELVPNLDSVPEELHLDSTGRLVPINVGPVCVD
                     YDTGWFAERGIAPPTSFEELADPQYASLLVLEDPVTSTPGLAFLMATGVHFGNGAEQY
                     WRSLLDGGALVVGGWSEAWYEQYSVSGGERPLVLSYASSPPAEVFYSDGEVAEPASGV
                     VEATCVDQVEFAGVLAGSAHEELATRVLNEMLSLEWQQSLPLANFVYPARPDAELPEL
                     FVQFAPRPSSSVMIDPAVVDEHRDDWIDTWRSIAG"
     gene            1253..>2885
                     /locus_tag="PLP95_09330"
     CDS             1253..>2885
                     /locus_tag="PLP95_09330"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HPB46041.1"
                     /translation="MTWRRAVLGVGAVLWALAYGWPLVAVMGRSITGVAGREGLGLAT
                     WSVLWDERTLRLFAITFAQSAASVVVVMALGVPMGWALARLSFRGSSVVSAVVMVPFV
                     LPTLTVASAVLAVVGGLPDADGARFALVVVAHVCLNLGLMVRAVAAAVAAVPASIEDS
                     ARSLGHRPLRAVRATTLRMIAPQIADAALLVAMFCLTSFGVIVGLGSARVSTVEVEVW
                     FLSTRSLDLASAAVLVVAQLAIVLAIVALQARVGAQRRTRVGMGVGAVRRRPVRGRGD
                     RSLAAFTVLATAVFAGGPPLALVLRSLHTARGWGLGNYRALFGAGQHRPDRSIPALAT
                     TILGPGDAGGAISASVLGAVLGAVLALALAAPLVVSAGRDDSVGRFVDRLLIVPMSIS
                     AATLGLGFLLAYSGAWIDLRGTWIAVPLVQAATAAPIAARLLLGGVRSVDRQPFEAAA
                     VLGAGRLRRGLRIGVPLLGRPVAVAAGFAFAMAIGEFGATVFLARPDRPTIPILISRL
                     LGRAGTGNFGAAMALSCVLAAIVIGMPPANDGGGGS"
BASE COUNT          334 a          912 c         1105 g          534 t
ORIGIN      
        1 gttgcgctcg catcggctcc gcgccgggtc gaccctgggc atcagcaacg aagcgctcgg
       61 tggggaagtt gtcgtgtccg tcgacgaagg agtgaccctc gtcgtgattc cagatgcaga
      121 ggtgctctca tgagtcgaat ccaacgtcgt acaaggtccg ggtggtctcg ccggttcgtc
      181 ccggtcgtgg gggcgatggc gatgctcgcc acgatcgcgt ccggttgcgg cgaccccgac
      241 gattcagccg accggcccaa cgacgagggt gcacagcggc ccaccatcac catcgtcacc
      301 tacgacagct acgtgttgga tcccgaggtc gaggcggcgg ttgaggaggc gctcgatgtc
      361 gacctcgtca tcagcgcgag cggtgacggc gccgaggcgc tcgcggcggc gatcctgacc
      421 gcgggccgcc ctgcgggcga catcttcttc ggggtcgaca acgtggtgat gagccgggcg
      481 ttgtcggaac ccgtgttcgt cgaattcgac cgcgaactgg tgccgaacct cgactcggtc
      541 cccgaggagc tccacctcga ctccaccggt cgcctcgtgc cgatcaacgt cggtccggtg
      601 tgtgtcgact acgacaccgg ctggtttgcc gaacgcggca tcgcaccgcc gacctcgttc
      661 gaggagctcg cggatccgca gtacgcgtcg ctgctggtgc tcgaagaccc ggtgacctcc
      721 acgccgggcc tggcgttcct gatggcgacc ggtgttcact tcggcaatgg ggccgagcag
      781 tactggcgct cgctcctcga cggtggggcg ctggtggtcg gagggtggag cgaggcgtgg
      841 tacgagcagt acagcgtgag tggcggcgaa cgtccgctgg tgctcagcta cgcgtcgagc
      901 ccgccggcgg aggtgttcta ttccgatggc gaggtcgccg aaccggcctc gggggttgtc
      961 gaggcgacct gtgtggacca ggtcgaattc gccggggtgc tggcgggatc ggcgcacgag
     1021 gagttggcga cgagggtgct caacgagatg ttgtcgctgg agtggcaaca gagcctgccg
     1081 ctcgcgaact tcgtgtaccc ggcgcggccc gatgccgaat tgcccgagtt gttcgttcag
     1141 ttcgctcccc gcccgtcgtc gagcgtgatg atcgacccgg ccgtggtcga cgagcaccgc
     1201 gacgactgga tcgacacctg gcggtcgatc gccggatgac ggtggtcgtg tgatgacgtg
     1261 gcggcgagcg gtgctcggcg tgggcgcggt gctgtgggcg ctggcctatg gctggccgct
     1321 cgtcgccgtc atgggtcgca gtatcaccgg tgtcgccggg cgcgaggggc ttggactcgc
     1381 cacgtggtcg gtgctgtggg acgaacgcac actgcggctg ttcgccatca ccttcgctca
     1441 gagcgcggcg tcggtcgtcg tggtgatggc gctgggggtt ccgatgggct gggcgttggc
     1501 gcgcctgtcc tttcggggaa gttcggtcgt ctcggcggtc gtgatggtgc cattcgtgtt
     1561 gcccacgctg acggtggcca gcgcggtgct cgccgtcgtg ggcgggttgc ccgacgccga
     1621 cggcgcccgc ttcgcgctcg tggtcgttgc gcacgtgtgt ttgaacctcg ggctgatggt
     1681 ccgggccgtc gcggcggcgg tcgccgcggt gccggcatcg atcgaggact cggcgcggtc
     1741 gttggggcac cgcccgctgc gggcggtccg cgcgacgacc cttcggatga tcgccccgca
     1801 gatcgcggat gctgcgttgc tcgtggccat gttctgcctc acctccttcg gggtgatcgt
     1861 tgggctcggc tccgctcggg tgtcgaccgt cgaggtcgag gtctggttcc tcagcacccg
     1921 cagtctcgat ctggccagcg ctgcggtgct ggtggtggca caactcgcga tcgtgctggc
     1981 catcgtcgcg ttgcaggccc gcgtcggggc acagcgccgt acgcgggtcg gcatgggtgt
     2041 gggtgcggtt cgccgtcgac cggtgcgtgg ccgcggcgac cggtcgctcg ccgccttcac
     2101 ggtgctcgcc accgcggtgt tcgctggcgg cccgccgttg gcattggtgc tgcggtcgct
     2161 gcacacggct cgaggctggg ggctgggcaa ctatcgggcg ctgttcggcg ctgggcagca
     2221 ccggccggac cggtcgatcc ctgcgctggc aacgaccatc ctgggtcccg gagacgccgg
     2281 tggagccatc agtgcgtcgg tgctgggagc ggtgctggga gcggtgctgg cactggcgct
     2341 ggcggccccg ctggtggtct cggcggggcg cgacgattcg gttggtcgct tcgtcgaccg
     2401 gctgttgatc gtgccgatga gcatctccgc cgcgaccctc ggactcgggt tcctcctcgc
     2461 ctacagcgga gcgtggatcg acctgcgcgg cacctggatc gcggtgccgc tcgtgcaggc
     2521 ggcgacagcg gcccccatcg ccgcgaggct gctgttggga ggggtgcgca gcgtcgaccg
     2581 acagccgttc gaagcggctg cggtgctggg agccggccgg ttgcggcggg ggttgcgtat
     2641 cggggttccc ctcctggggc ggccggtggc cgtggctgcg gggttcgcgt tcgcgatggc
     2701 catcggcgag ttcggagcga cggtgtttct ggcccggccg gatcgcccca ccatcccgat
     2761 cctcatcagc cgcctgctcg gccgggcggg aacgggcaac ttcggagcgg cgatggcgtt
     2821 gagttgcgtg ctggcggcga tcgtcatcgg gatgccgccg gccaacgacg gcggcggcgg
     2881 atcgc
//
LOCUS       DAOOMA010000344         2883 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_42256_length_2883_cov_3.024752, whole genome shotgun sequence.
ACCESSION   DAOOMA010000344 DAOOMA010000000
VERSION     DAOOMA010000344.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 2883)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2883)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2883
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42256_length_2883_cov_3.024752"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..814
                     /locus_tag="PLP95_09335"
     CDS             <1..814
                     /locus_tag="PLP95_09335"
                     /inference="COORDINATES: protein motif:HMM:NF014910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HPB46042.1"
                     /translation="CGSEGTLAVISAVRLRLIPEVGESVVALCSFADLDEAIGFAWEI
                     RQRVPGSEAIEYLDGACADLVAMTRRVALPVRGAAMILVEAAASVAPLDDLAVGIAAA
                     GSRPDDVAFGIEPSQRRELWRLRDELTIALAALGTVHKYDVSVPHDRISHVRERTEAG
                     LARLAPRSTAWWFGHVCDDNVHLNLTGPELGVGGAERVDESTVAPRSLDELVFGVVVD
                     AGGSISAEHGIGVHKVPYLHLARSATEIAHMRAIKAAFDPQSILNPGVILPG"
     gene            complement(840..1622)
                     /locus_tag="PLP95_09340"
     CDS             complement(840..1622)
                     /locus_tag="PLP95_09340"
                     /inference="COORDINATES: protein motif:HMM:NF015000.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HPB46043.1"
                     /translation="MASRFAVLDHPGPLAFAHRGGAGEWPENTMPAFEGAVSLGFRYL
                     ETDVHRTADGVLVAFHDDRLDRVTDRAGLISELTWDEVRRAKVGGREPIVRFEELLEA
                     FPAVRFNIDPKADDAVEPLARVLRDAKAIDRVCIGAFSDARLAEMRERCGPALCTSLG
                     PKQILRLRVAATRGFRSLVRPAFVQGAAQVPVSHKGVPLVTPAFVDTAHGLGMQVHVW
                     TIDDPVEIHRLLDLGVDGIMTDQPLELKRVLTSRGQWVHPAR"
     gene            1725..2582
                     /locus_tag="PLP95_09345"
     CDS             1725..2582
                     /locus_tag="PLP95_09345"
                     /inference="COORDINATES: protein motif:HMM:NF024762.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase family protein"
                     /protein_id="HPB46044.1"
                     /translation="MRCLEAFRQEVSNGSQARLDRLTIALAGVADPRVDLDAELARLD
                     RLAARFGAEGGRNGDDLIRWMARHGFRGNAREYYHPDNSMLPRVLRTGLGIPITLSIV
                     AIEIGRRHGLGLTGIGVPGEFIVGESLVCEPPAPNPPPVGRRRPRGAAAARFHNPYRS
                     RSFDLDGLHRFWRETNQEGLVTAPEVLWPVGPVDIVERILNNLEQIYLRRSAPRGLEL
                     VYRLRVELPSATVATRRRYAEILERSGNFAEGIRQIRVVMDERHPSENVLTTRRDWLL
                     LRRLQANLN"
     gene            2662..>2883
                     /locus_tag="PLP95_09350"
     CDS             2662..>2883
                     /locus_tag="PLP95_09350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HPB46045.1"
                     /translation="MPDPSEALVEERLEQLLAEHPPADTEPQRFLEAQYDLGLAWVHF
                     PEGVGGLGVSPKLQRNVIRRVSEAGGPIGG"
BASE COUNT          455 a          952 c          986 g          490 t
ORIGIN      
        1 gtgcgggtcc gaaggaaccc tggccgtcat ctcggcggtc cggttgcggc tgattcccga
       61 ggtgggcgag tcggtggtcg cgttgtgttc ttttgccgac ctcgacgagg cgatcgggtt
      121 cgcctgggag atccgtcagc gggtgcccgg cagcgaagcg atcgagtacc tcgacggggc
      181 ctgtgccgat ctcgtcgcca tgacgcgtcg tgtcgccctg ccggttcgcg gcgccgcgat
      241 gatcctggtc gaggcggccg catccgtggc gccgctcgac gatctggcgg tggggatcgc
      301 cgctgccggg tcgcggcccg acgatgtcgc cttcggtatc gagccatcgc agcgacgcga
      361 attgtggagg ctgcgcgacg agctcacgat tgcgctggcg gctctgggaa ccgtccacaa
      421 gtacgacgtg tcggtccctc acgatcggat atcccacgtc cgggagcgca ccgaagccgg
      481 acttgcccgc ctcgctccgc gcagcaccgc ctggtggttc ggtcacgtgt gcgacgacaa
      541 cgtgcatctc aacctcaccg gtccagaact cggcgtcgga ggcgccgagc gggtggacga
      601 gtcgacggtg gctccgcgat cgctcgatga gctggtgttc ggcgtcgtgg tcgatgcggg
      661 agggtcgatc agtgccgaac acggaatcgg agtccacaag gtcccgtatc ttcatctcgc
      721 ccgttccgcc accgagatcg cgcacatgcg ggcgatcaaa gcggcgttcg accctcaatc
      781 gattctgaat cccggggtga ttctcccggg ctgatccctc cggactgtcg ccgcaagcgt
      841 catcgcgcgg ggtgaaccca ctgcccgcga gatgtcagta cgcgtttgag ttcgaggggt
      901 tgatcggtca tgatgccgtc gaccccgagg tcgaggagcc ggtgaatctc gaccgggtcg
      961 tcgatggtcc acacgtggac ctgcatcccc aggccgtggg cggtgtccac gaaggccggg
     1021 gtgacgaggg gtaccccctt gtgggagacc ggcacctgag cggccccctg gacgaacgcc
     1081 ggcctcacca gcgatcgaaa cccccgtgtc gcagcaacac gaaggcgcag gatctgcttc
     1141 ggcccgagcg aggtgcacag cgcgggccca catcgctcgc gcatctcggc caggcgggcg
     1201 tcgctgaagg caccgatgca cacccggtcg atggccttgg cgtcgcggag aaccctcgca
     1261 agcggctcga ccgcgtcatc ggccttcggg tcgatgttga accgcaccgc gggaaacgcc
     1321 tccagtaact cctcgaaccg cacgatgggt tcgcggccgc cgaccttggc gcggcgaacc
     1381 tcatcccagg tgagctccga gatcagcccg gcccggtcgg tgacacggtc gaggcggtcg
     1441 tcatggaagg cgaccaggac accgtcggcg gtgcggtgca cgtcggtttc gaggtagcgg
     1501 aagccgaggc tgaccgcacc ctcaaacgcc ggcatggtgt tttcgggcca ttcgcctgca
     1561 ccccctcggt gtgcgaaggc gagtgggcca ggatgatcga gtacggcaaa tcggctggcc
     1621 atcgggttct ttctactccg cggcgacaac gccggcgaga catcccggcc catcgcggcg
     1681 agacagcacg cacgcgagcc ccctcacggg ttaccgtcgt ggcgatgcgt tgcctcgagg
     1741 cgtttcgaca agaggtgtcc aacggcagcc aagcgcgtct ggatcgactg acgatcgcgc
     1801 ttgccggcgt ggccgacccg cgtgtcgacc tcgacgccga actcgcgcga ctcgaccgcc
     1861 tcgccgcccg gttcggcgcc gaagggggac gcaacggcga cgatctgatt cggtggatgg
     1921 ctcgtcacgg attccgtggg aacgcccgcg agtactacca cccggacaat tcgatgcttc
     1981 ctcgggtgtt gcgcaccggc ctgggcattc cgatcacgct cagcatcgtc gccatcgaga
     2041 tcggacgacg ccacggcctc gggctcaccg gaatcggtgt ccccggcgag ttcatcgttg
     2101 gggaatcgct cgtgtgcgaa cccccagctc ccaatccgcc accggtcggc cggcggcggc
     2161 cacgcggcgc cgctgccgca aggtttcaca atccgtaccg atcgcgctca ttcgacctcg
     2221 atggcttgca tcgtttctgg cgcgagacca accaggaggg cctcgtgacc gcccccgagg
     2281 tgttgtggcc cgtggggccg gtggacatcg tcgaacgaat cctcaacaac ctcgaacaga
     2341 tctacctacg ccgcagcgcg ccgcggggtc tcgaattggt gtatcgcctc cgcgtcgaac
     2401 tgccgagcgc gacggttgcc acccgacgtc gatatgcgga gatcctcgag cgctccggca
     2461 actttgctga ggggatccgc cagattcgcg ttgtgatgga cgagcgccat ccttcggaga
     2521 acgtgttgac gacccgccgc gactggctgt tgttgcggcg tctccaggcg aatctcaact
     2581 gagctacctt gaccactgga gcgccgccgg ggggcggtgc gggtcgtggg ttcgcccatg
     2641 aggggagtgt gaacgatgac catgccggat ccgagtgagg cgctggtcga ggagcgtctc
     2701 gagcagttgt tggccgaaca cccgcctgcc gacaccgaac cgcagcgatt cctggaggcc
     2761 cagtacgacc tcggactcgc gtgggtgcat tttcccgagg gcgtcggcgg cctcggggtc
     2821 agcccgaagt tgcagcgcaa cgtcatccgg cgggtgtccg aggccggcgg gccgatcggc
     2881 ggg
//
LOCUS       DAOOMA010000345         2881 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_42300_length_2881_cov_3.171975, whole genome shotgun sequence.
ACCESSION   DAOOMA010000345 DAOOMA010000000
VERSION     DAOOMA010000345.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 2881)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2881)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2881
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42300_length_2881_cov_3.171975"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            291..1511
                     /locus_tag="PLP95_09355"
     CDS             291..1511
                     /locus_tag="PLP95_09355"
                     /inference="COORDINATES: protein motif:HMM:NF014010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA C-terminal domain-containing protein"
                     /protein_id="HPB46046.1"
                     /translation="MLAEAKDTSELMVDLAYAAVYFNDPGMCEEVADLEERLNDLVQD
                     MRAVCILAVRRPAEAESMASVLQVIGAIEGIGNAAIDITRIVTHRLGIPRELIADLSN
                     AEEVSHRVWIREGSHMANRPIASLELTVQTGMRIMAIRRGRSWITEIDGDIVLVPGDV
                     LFLRGSPPGIVRLHELAAAPTWNPPDASSGAALTDLDRAVDVLVEMKNMSEAAVGLAY
                     SALALRDRGLAAEVSHLATRLDEMKDHLQLWVLRASSSDVDPSPLRGLLQLSHVAEEL
                     GNQASQMVWLISDDVEFHPVLAMALGEADVVVMRVPVAAASTVVGETLATMQLDTEPG
                     FAILAIRRGGQYLYHPRPFVAFEVGDEVIAEGPYEGRQRLAELFGWHLRDDDDDDSPG
                     ELELVPLRGTDGAR"
     gene            1511..2452
                     /gene="mca"
                     /locus_tag="PLP95_09360"
     CDS             1511..2452
                     /gene="mca"
                     /locus_tag="PLP95_09360"
                     /inference="COORDINATES: protein motif:HMM:TIGR03446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010126 - mycothiol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mycothiol conjugate amidase Mca"
                     /protein_id="HPB46047.1"
                     /translation="MSVPADDRPEGGVGNGRSSLTQPCDPNDVPLALLTVHAHPDDEA
                     SKGSALVAKYVALGVRATLVCCTGGELGSVLNPAMDREEVHADLAGVRRKELERSAQI
                     IGFSDVDLLGYRDSGMPDMEENHDPSNFWNAPLNEATARLVASIRRARPQVMITYGDD
                     QGGYPHPDHLKVHTVSVEAFDRAADESYRPDLGAAWQASKLYYSVWSRRRGLALHERM
                     VELGVESPFTPEWLDRFNQDERITTSVDVGEFMEVRDASLRAHATQVDPNEAFWFGIN
                     PREQARAYRWDDYILARSLVDTDLPEDDLFAGIVASW"
     gene            2477..>2881
                     /locus_tag="PLP95_09365"
     CDS             2477..>2881
                     /locus_tag="PLP95_09365"
                     /inference="COORDINATES: protein motif:HMM:NF016668.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChbG/HpnK family deacetylase"
                     /protein_id="HPB46048.1"
                     /translation="MTTLAQRLGFADDERVVIVTCDEFGLSHASNEGCLAALDGVATS
                     ASLLVPAPWSRSAVAYSVGLDVGVGLTLNSPFDRYRWGPITHAPSLLDGDGGFPRTLD
                     DSWDHADLDEVRRECRAQIERAGLWGLEVSHVS"
BASE COUNT          464 a          886 c          997 g          534 t
ORIGIN      
        1 gatggcgagg agccacgcgg cgtcgctcct ggcggccgga ggtgaaggct gaggttcgtt
       61 gtcgtcggtt gcgatctcca tggcgacccc ctcggagcgg ttggtgactc gcgtcacatc
      121 tccgtcgggt gcaggttacc gtcgctgcga ccggcggtgg agatcgccgg cgtgtcgatg
      181 atgcggcacc acgtcggcgg aacaggtgag cgtggccggt gcgcgcctgc ggcgcaatcc
      241 gttacgctcg ggggtcttct atggatgatc gacctcgaag tctgaggacc atgttggcgg
      301 aggcgaagga cacctccgaa ctcatggtcg accttgccta tgcagccgtc tacttcaacg
      361 accccgggat gtgcgaagag gtcgccgatc tcgaggagcg gctgaacgat ctcgttcagg
      421 acatgcgggc ggtgtgcatc cttgcggtgc gtcgacccgc cgaggccgaa tcgatggcct
      481 cggtgctgca ggtgatcggc gccatcgagg gcatcggcaa cgctgcgatc gacatcaccc
      541 ggatcgtgac ccatcgtctc gggatcccac gcgaactgat tgcagacctg tccaacgccg
      601 aggaggtgag ccaccgggtg tggatccgcg aggggtctca catggcgaac cgtccgatcg
      661 cctcgctcga gctcaccgtt cagaccggaa tgcggatcat ggcgattcgt cgcggccgca
      721 gttggatcac cgagatcgat ggcgacatcg tgttggtgcc cggagacgtg ttgtttcttc
      781 gcgggtcacc cccgggcatc gtgcggttgc acgagctcgc ggcggccccc acctggaatc
      841 cgcccgacgc ctcctcagga gctgcactca ccgatctcga ccgagcggtt gacgtgctgg
      901 tcgagatgaa gaacatgtcc gaagcagcgg tcggcctggc gtattccgcg ctcgcacttc
      961 gcgaccgcgg actcgcggcg gaggtgtcac atctcgccac acgcctcgac gagatgaagg
     1021 atcacctgca gttgtgggtg ttgcgcgcga gttcgagcga tgtcgacccg tcaccgcttc
     1081 gcggcttgtt gcagttgagc cacgttgcag aggaactcgg aaaccaggcg agccagatgg
     1141 tgtggctcat cagtgacgac gtcgagttcc acccggtgtt ggcgatggcc ctcggcgagg
     1201 ccgatgtggt ggtcatgagg gttccggtcg cagcggcatc gacggtggtc ggcgagacgc
     1261 ttgctacgat gcagctcgac accgaaccgg gatttgccat cctggcgatt cgccgcggcg
     1321 gccagtatct gtatcacccc cggccatttg tggcattcga ggttggcgac gaggtgatcg
     1381 cggaggggcc atatgagggg cgccaacggc tggctgagct cttcggctgg catctacgcg
     1441 acgacgatga cgacgattct ccgggcgaac tcgaactggt tcccctccgg ggcaccgacg
     1501 gtgcccgctg atgtcggtgc ccgctgatga tcggcccgag ggcggggtcg ggaacggcag
     1561 gtcgtcgctg acgcagcctt gcgatccaaa cgacgttccg ctcgcgttgt tgaccgtgca
     1621 cgcccacccg gacgatgagg cctcgaaggg ttcggcgctc gtcgcgaagt acgtcgcgct
     1681 cggagtccga gcgacgctgg tgtgttgtac cggcggcgaa ctcggctccg tcctcaaccc
     1741 ggcgatggat cgtgaagagg tgcacgccga cctggcgggg gttcgtcgca aagagttgga
     1801 acggtcggcg cagatcatcg ggttcagcga cgtcgatctg ctcggatacc gcgactcggg
     1861 aatgcccgac atggaggaga accacgaccc gtcgaacttc tggaacgcgc cgttgaacga
     1921 ggccacggca cggctcgtgg cgtccattcg tcgggcgcgg ccgcaggtga tgatcaccta
     1981 cggcgacgat cagggcggat atccgcaccc cgatcacctc aaggtgcaca cggtgagcgt
     2041 cgaggcattc gaccgggccg cagacgagtc gtaccggccc gacctgggcg ctgcgtggca
     2101 ggccagcaag ctctactact cggtatggtc tcgccggcgc ggcctcgccc tgcacgagcg
     2161 gatggtggaa ctgggcgtcg agtcgccgtt cacgccggag tggttggacc ggttcaacca
     2221 ggacgaacga atcacgacct cggtcgatgt cggcgagttc atggaggtgc gcgacgcctc
     2281 gctgcgggca cacgccaccc aggtcgatcc caatgaggcg ttctggttcg gtatcaaccc
     2341 gcgcgaacag gctcgcgcct accggtggga cgactacatc ctcgctcggt cgctggtcga
     2401 caccgatctg cccgaagacg accttttcgc gggcatcgtg gcttcctggt gatcgacgag
     2461 ttgtaggttg gggaccgtga cgacgctcgc tcaacgcctt ggattcgccg acgatgaacg
     2521 ggtcgtcatc gtgacctgcg atgagttcgg cctgagccac gcttccaacg agggttgtct
     2581 tgccgcgctc gacggggtgg ccacgagtgc atcgttgctc gttccggctc catggtcacg
     2641 gtccgcggtc gcatattcgg tcggcctcga tgtcggggtg gggttgaccc tgaactcgcc
     2701 gttcgatcgc tatcgctggg ggccgatcac ccacgctccc tcgctgctcg acggcgacgg
     2761 agggttccct cgaacgctgg atgattcgtg ggaccatgcc gacctcgacg aggtacgtcg
     2821 cgagtgccgg gcccagatcg agcgcgccgg gctgtggggg cttgaggtgt cccatgtgtc
     2881 g
//
LOCUS       DAOOMA010000346         2878 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_42362_length_2878_cov_3.049238, whole genome shotgun sequence.
ACCESSION   DAOOMA010000346 DAOOMA010000000
VERSION     DAOOMA010000346.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 2878)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2878)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2878
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42362_length_2878_cov_3.049238"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..265
                     /locus_tag="PLP95_09370"
     CDS             <1..265
                     /locus_tag="PLP95_09370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012223078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HPB46049.1"
                     /translation="IVGENSRTDDMDVNIVREKKLTNMRASGSDHTVALVPPRHLSLE
                     QALEFIESDECVEVTPNHVRLRKVELNATERAKLTKAAKHGRG"
     gene            302..1030
                     /locus_tag="PLP95_09375"
     CDS             302..1030
                     /locus_tag="PLP95_09375"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HPB46050.1"
                     /translation="MVSGSGTVGGRATPQRSDSLKTQSLLLTTAERLFAERGIDAVSL
                     SEINRAAGQRNKSALHYHFGSRDGVLKALLERHFDAVSHLRDALVAGRDPSHEPTLRE
                     AVAVIVLPLADHVADPDSGGIHYVGIMAQLAANPSTPIAVEIFNRQRSAIAAIAPTLS
                     RHIPQAPPPLRARRAQLLNSSLFNALLVQAHSTHSEPDRAVYVNDLIDMLAGLLSAPI
                     APESQPEVDRFAERYEQRTAGASS"
     gene            1091..1750
                     /gene="nucS"
                     /locus_tag="PLP95_09380"
     CDS             1091..1750
                     /gene="nucS"
                     /locus_tag="PLP95_09380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease NucS"
                     /protein_id="HPB46051.1"
                     /translation="MRLVIARCSVDYQGRLSAHLPLATRLLMVKADGCVAIHADGGAY
                     KPLNWMNAPNRLSEGDGTWTVTNPKGETLTITIEEILHDTAHDLGIDPGLQKDGVEAH
                     LQELLAANTDAITAGLTLVRREFPTDIGPVDLLCRDENGTAIAIEVKRRGEIDGVEQL
                     SRYLERLDRDPTLRGVQGIFVAQIIKPQAKVLASSRNIRCVEVDYDALRGIESNELRL
                     F"
     gene            1850..>2878
                     /locus_tag="PLP95_09385"
     CDS             1850..>2878
                     /locus_tag="PLP95_09385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB46052.1"
                     /translation="MSRPKALVLAALSTPVLLYLVLLGAWALFGRSDPGTVASSVHLV
                     GVDIGGQNREELNSTLEDISSEFAQTAVDIQIPDATLSTNAGQLGLSLDSERTVRAAM
                     RPGHADPLGIAPIRWAMSWFSPRKVDVAIDIDDDAAQATFSELEADHRVHPIEPTMAV
                     DSTTFHLVQGQDGSGIDLTDVLTRLPSAIDSIGETITLSAEPVTFPPKLGDDAVQAVV
                     DQANKITAGSLDITAGDESFTLTGDNLAGALSMSADGDTAELAVDHDIINLQLVSNLS
                     IPANPTGVSFDIVNGVPTPVPGHDAQVCCQTDAADRITEALLSAQPSVELATRTITAA
                     EGVEWASTL"
BASE COUNT          550 a         1010 c          861 g          457 t
ORIGIN      
        1 gatcgtcggc gagaacagcc gaaccgacga catggacgtc aacatcgtcc gcgagaagaa
       61 gctcaccaac atgcgagcct ccgggtccga ccacaccgtg gcactcgttc cccctcgcca
      121 cctcagcctc gaacaggccc tcgaattcat cgaatccgat gagtgtgtcg aggtcacgcc
      181 gaaccatgta cggttgcgca aggttgaact gaatgccact gaacgagcga agctgacaaa
      241 ggcggccaag cacggcaggg gctgagtccg ctagggggcg gacagagcaa gcgggagcag
      301 catggtgagc gggagcggca cggtgggtgg cagagccaca ccccaacgat cggacagtct
      361 caagacccag tcgctgctct tgaccaccgc cgagcggctt tttgccgaac gcggcatcga
      421 cgcggtctcc cttagcgaaa tcaatcgggc agccggccag cgcaacaagt cggcgttgca
      481 ctatcacttc ggcagtcgag acggcgttct caaggccctt ctcgaacgtc acttcgacgc
      541 cgtgtcacac ctgcgtgacg cgctggtcgc cgggcgtgac ccgagccacg aacccaccct
      601 tcgagaagcc gtagcggtga tcgtgttgcc ccttgccgac cacgtcgcag atccggacag
      661 cggcggcatt cactacgtgg gcatcatggc ccagctcgca gccaacccgt ccacgccgat
      721 cgccgtggag atcttcaacc gccaacgttc cgctatcgcc gccatcgcgc cgacgctcag
      781 tcgccacatc ccccaggcgc ctccgccgtt gcgcgcccga cgtgcccaac tcctcaactc
      841 cagcttgttc aacgcgttgc tggtgcaggc ccacagcacc cactcggagc cggaccgggc
      901 ggtgtatgtg aacgacctca tcgacatgct ggccggcctg ctcagcgccc cgatcgcacc
      961 ggaatcgcaa ccggaggtcg atcgattcgc cgagcgctac gagcagcgaa cagccggcgc
     1021 gagcagctga cgagcactga cgcgcactga cgcgaatcag cgtcccgcgc gactcattag
     1081 tctctccgag gtgcgtctcg tcatcgcccg ttgctcggtg gactatcaag gccgcctgag
     1141 cgctcacctt cccctcgcca ctcgcctcct catggtcaag gccgatggct gcgtcgcgat
     1201 ccacgcggac ggcggggcgt acaagccgct caactggatg aacgcaccga atcgcctgag
     1261 cgagggcgac ggcacttgga cggtgacgaa tccgaagggc gaaaccctga cgatcaccat
     1321 cgaagagatc ctccacgaca ccgcacacga cctcggcatc gatcccgggc tgcaaaagga
     1381 cggcgtcgaa gcgcacctgc aggagctcct ggccgccaac accgacgcga tcaccgctgg
     1441 gctgacgctg gtgcgacgcg agttcccgac cgacatcggc ccggtcgacc tgctgtgtcg
     1501 agacgagaac ggaacggcca tcgccatcga ggtcaagcga cgcggcgaga tcgacggcgt
     1561 cgaacagctg tcgcggtatc tcgaacggct cgatcgcgat ccgaccctgc gcggcgtgca
     1621 gggaatattc gtcgctcaga tcatcaaacc ccaggcgaag gtgctggcat cgagccgcaa
     1681 catccgctgc gtcgaggtcg actacgacgc gttgcgcggc atcgaatcca acgagttgcg
     1741 gttgttctga tctcgtccgg gtcgctctcc tgaggcgatt ctccggattc cgccctgaat
     1801 tgcgcccgat tggcgcgatc gcggcgcgaa cctgcgcgaa tctggagggg tgtcgcgtcc
     1861 caaagctctc gtcctcgccg cgctgtcgac gccggtgttg ctctatctcg tcctcttggg
     1921 agcctgggcg ctgttcggga ggagcgatcc gggcacggtc gccagctcgg tgcatctcgt
     1981 cggagtcgac atcggtgggc agaaccgcga ggagctcaac tcgacactgg aagacatctc
     2041 ctcggagttc gcgcaaaccg ccgtcgacat ccaaatcccc gacgcgacgc tgtcgacaaa
     2101 cgccggacag ctggggctga gcctcgattc cgaacgaacc gttcgcgccg cgatgcgccc
     2161 cggccacgcc gacccgctcg ggatcgcacc cattcgctgg gccatgtcgt ggttctcgcc
     2221 gcgcaaggtc gacgtcgcga tcgacatcga cgatgatgcc gcgcaggcga ccttttccga
     2281 gcttgaggcc gatcatcggg tccatccgat cgaaccgacg atggccgtcg acagcaccac
     2341 gtttcatctt gtccaaggcc aggacggttc gggcatcgac ctcaccgacg tcctcacacg
     2401 gctgccgagc gccatcgact cgatcggcga gaccatcacc ctgtcggccg aacccgtcac
     2461 cttcccgccg aaactcggcg acgacgcggt ccaggccgtc gtcgatcaag ccaacaagat
     2521 caccgcgggc tccctcgata tcaccgcggg cgacgaatcg ttcacgctga ccggcgacaa
     2581 cctcgccggt gcactgtcga tgtccgcaga cggcgacacg gccgaactcg ccgtcgacca
     2641 cgacatcatc aacctgcaac tcgtctcgaa cctgtcgata cctgcgaacc cgacgggggt
     2701 gagctttgac atcgtcaacg gtgtcccgac cccggttccg ggccacgatg cccaggtgtg
     2761 ctgtcagacc gacgccgctg atcgcatcac cgaggcgctc ctcagcgcgc agccctcggt
     2821 cgagctggca acacgaacga tcaccgcggc cgagggtgtc gagtgggcat cgaccctc
//
LOCUS       DAOOMA010000347         2861 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_42702_length_2861_cov_3.973628, whole genome shotgun sequence.
ACCESSION   DAOOMA010000347 DAOOMA010000000
VERSION     DAOOMA010000347.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 2861)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2861)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2861
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42702_length_2861_cov_3.973628"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1620
                     /locus_tag="PLP95_09390"
     CDS             <1..1620
                     /locus_tag="PLP95_09390"
                     /EC_number="2.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016744 - transketolase or transaldolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HPB46053.1"
                     /translation="TTGPLGQGIANAVGMAIAERSTRASFGEAVSNHHIWCIAGDGDL
                     SEGVSHEAASFAGHQRLDNLTVIYDDNHISIDGPTELALDDNAVGRFRAYGWNVVDLG
                     EAAEDLDALESALNDARAHRGGPTLIVVRSHIGFPSPTLTDSASAHGLAFKAADIEAT
                     KAVMGLPVDESFHLPDEVLAAYRNAGVRGGEARAAWEAALPAALGDRRDDYESRQSLT
                     GLAGWTDALPTFEVGESVATRVALAKVLNSLVDSVPAIAGGGADLTGNTGTELKGQTP
                     MSAEAPDGRQIHFGVREHAMGATMNGMALHGGILPVGGTFLVFSDYMRPSVRLAALMG
                     TKVLFAWSHDSVGVGEDGPTHQPVEHVASLRTIPGLDVMRPADANETAHAVRLMIEGD
                     APMALIMSRQNLPVLSGTSYDGVAKGAYALLDPSDAQITLVATGSEVSVAIDAAAELA
                     SAGIVARVVSMPSWERFEAAGRAYQESVIPSGVPSLSIEAGVTFGWHRWVDDAIGIDR
                     FGASAPGDVVMDRLGINVANVVARATELVGN"
     gene            1672..2775
                     /gene="tal"
                     /locus_tag="PLP95_09395"
     CDS             1672..2775
                     /gene="tal"
                     /locus_tag="PLP95_09395"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012228740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004801 - transaldolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HPB46054.1"
                     /translation="MGRLHELFDEQGQSPWLDNLKREWLHSGEIQRWIDRGVRGITSN
                     PAIFQKAIESATEYDEQFSALIAGGASVEDAYWELVISDINDALGLLRSTHDASGGAD
                     GFVSVEVSPALAHDSAATEAAARDLAERIDAPNLMVKIPATRAGLGPISTMIAEGHSI
                     NVTLIFSLERYAEVMEAYLSGLEAAVAAGVDDLSTISSVASFFISRVDTEVDKRLDAI
                     GTDEALSLRGHAAFVQGQLAYEMFRSTFSGDRWEALAAKGARPQRPLWASTSTKNPAY
                     ADTLYVDTLIGPDTVNTVPDATLDAFEDHGTVARTVDGDVEGAHQVWKSVGALVDLDA
                     VAEQLEREGVASFISAYDDLLAVLAAKAQQLTS"
BASE COUNT          451 a          970 c          956 g          484 t
ORIGIN      
        1 accacgggtc cgctcggcca gggcatcgcc aacgcggtcg gcatggcgat cgccgagcgt
       61 tcgaccaggg catcgttcgg agaggcggtg tcgaaccatc acatctggtg catcgccggc
      121 gacggcgacc tgtccgaagg cgtgagccac gaggccgcat cgttcgccgg ccaccagcgt
      181 ctcgacaatc tgacggtcat ctacgacgac aaccacatct cgatcgacgg tccgaccgaa
      241 ctcgcgctcg acgacaacgc ggtgggccgg ttccgtgcct acgggtggaa cgtcgtcgat
      301 ctcggcgaag cggccgagga cctcgacgcc ctcgaatcgg cgctcaacga tgcccgtgcc
      361 caccgtggcg gtcccacgct gatcgtggtg cgcagccaca tcgggttccc ctcgcccacc
      421 ctcaccgact cggcctcggc acacggcctc gccttcaagg ccgccgacat cgaggccacc
      481 aaggccgtga tggggcttcc ggtcgatgag tcgttccatc tgcccgacga ggtgctcgcc
      541 gcctatcgca acgccggcgt ccgaggcggc gaggcccggg cggcgtggga ggcggcactt
      601 ccggcagcgc tcggcgaccg ccgcgacgat tatgagtcgc gccaatccct caccggtctc
      661 gccggctgga cagatgcact tcccaccttt gaggtcggtg agtcggtggc gactcgtgtc
      721 gcactcgcga aggtcctcaa ctccctcgtc gattcggttc cggcaatcgc cggtggtggt
      781 gccgatctca ccggcaacac cggcaccgaa ctcaaggggc agacgccgat gtcggccgag
      841 gcccccgacg gccgtcagat tcacttcggc gttcgagagc atgcgatggg cgccaccatg
      901 aacggcatgg cgcttcacgg gggtatcttg ccggtcggcg ggaccttcct ggtgttctcc
      961 gattacatgc gtccgtcggt gcgcctcgcc gcgctgatgg gaaccaaggt gctgttcgcc
     1021 tggtctcacg actcggtggg cgtgggcgag gacggtccga cccatcagcc ggtcgaacac
     1081 gtggcctcgc ttcgaaccat ccccggcctc gacgtgatgc gcccagccga tgcgaacgag
     1141 acggcccacg cggtgcgcct gatgatcgag ggcgacgcac ccatggcgct gatcatgagc
     1201 cgtcagaacc tgccggtgct ctcgggcacg agctacgacg gtgtggcgaa gggggcctac
     1261 gcgctcctcg acccgtcgga tgcgcagatc acgctggtgg ccaccgggtc ggaggtgtcg
     1321 gtagccatcg acgcggccgc cgagctggcc tcggccggga tcgttgcccg cgtggtgtcg
     1381 atgccgagtt gggagcgttt cgaggccgcc gggcgtgcgt atcaggagtc ggtcatcccc
     1441 tcgggggtcc cgtcgttgtc gatcgaggcg ggcgtgacct tcggctggca tcgttgggtc
     1501 gacgacgcga tcggcatcga tcggttcggc gcatcagccc ccggtgatgt cgtcatggat
     1561 cgcctcggca tcaacgtcgc caacgtcgtc gctcgggcca ccgaactcgt cggcaactga
     1621 tcctgtcccg cacagatctc gccgccataa cgcccaggaa cggagcacca catgggtcgc
     1681 ctacacgaac ttttcgatga gcagggccag agtccgtggc tcgacaatct caagcgcgag
     1741 tggttgcact ccggcgagat ccagcgttgg atcgaccgcg gcgtgcgcgg catcacgtcc
     1801 aacccggcca tctttcagaa ggcgatcgag tcggccaccg agtacgacga acagttctcg
     1861 gcgctgatcg ctggtggggc gtcggtggaa gacgcctact gggaactcgt gatcagcgat
     1921 atcaacgatg cgctcggcct gctgcgctcc acccatgacg cctcgggtgg cgccgacggt
     1981 ttcgtcagcg tggaggtttc cccggcactg gcgcacgatt ctgcggccac cgaggcggcg
     2041 gcgcgcgacc tcgccgagcg catcgatgct ccgaatctga tggtgaagat tcccgccacc
     2101 agggccggtc tggggccgat ctcgacgatg atcgccgagg gccactcgat caacgtcacg
     2161 ctcatcttca gcctcgagcg ttacgccgag gtgatggagg cctacctgag cgggctcgag
     2221 gcggcggtgg ccgcgggggt cgacgacctg tccacgatct cgtcggtggc gtcgttcttc
     2281 atcagccggg tcgacaccga ggtcgacaag cgcctcgatg cgatcggcac cgacgaggcg
     2341 ctgtcgttgc gcgggcacgc tgcgttcgtg cagggtcagc tcgcctatga gatgttccgc
     2401 tcgacgtttt cgggcgatcg ctgggaggcg ctcgcggcga agggtgcgcg tccgcagcgt
     2461 cccttgtggg cctcgacctc gaccaagaac ccggcctatg ccgacacgct gtatgtcgac
     2521 accctgatcg gccccgacac ggtcaacacc gtgccggacg cgaccctcga cgcgttcgag
     2581 gatcacggca cggtggcgcg cacggtcgat ggggatgtcg agggtgcaca ccaggtgtgg
     2641 aagtcggtcg gcgcactcgt cgatctcgac gcggtggccg aacagcttga gcgcgagggc
     2701 gtggcgtcgt tcatctcggc ctacgacgac ctgctcgccg tcctcgccgc caaggcccag
     2761 caactcacgt cctagcccgc cccgccgcga gggagagcct gccccgccgc ccgttctccg
     2821 ataaccagtg gtcgtgatca cgaccactgg ttatcggaga a
//
LOCUS       DAOOMA010000348         2855 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_42830_length_2855_cov_3.314286, whole genome shotgun sequence.
ACCESSION   DAOOMA010000348 DAOOMA010000000
VERSION     DAOOMA010000348.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 2855)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2855)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2855
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42830_length_2855_cov_3.314286"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            147..842
                     /locus_tag="PLP95_09400"
     CDS             147..842
                     /locus_tag="PLP95_09400"
                     /inference="COORDINATES: protein motif:HMM:NF012451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HPB46055.1"
                     /translation="MGGNSYYEVIGVRRDAGVEEIRHAYLHAARQAHPDVAGPSGEAR
                     MRELNEAWATLRDEEKRAFYDLSIPDLPAPAAGGRAGDESRATRVRTTPLPRAGRVGA
                     DYHWPTPPHHLDFSGLHPNQRSGPPPTGRPGGAQFGGGEQFTPPSPTPVDQTVGRGTL
                     GRGTLGRGTRFAPLVLFAIGGLTGACGAATSSPSLLVIAAVVVVVSALGALIVRLASA
                     TTAKERGVGSQNR"
     gene            856..1674
                     /locus_tag="PLP95_09405"
     CDS             856..1674
                     /locus_tag="PLP95_09405"
                     /EC_number="4.1.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004425 - indole-3-glycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol phosphate synthase TrpC"
                     /protein_id="HPB46056.1"
                     /translation="MATYLDRILEFHRARAAADRRSLDELMHRAAAMEAPRGFRDALL
                     GSTAETADATLAVISEVKRRSPSKGDLFAELDPAGLAQTYEAGGAACLSVLTDEHHFG
                     GSANDLAQARAAVSLPVLRKDFTVCALDVVDARLMGADCVLLIVAALSDEELANFDDL
                     AAQLGLDVLVETHDEAEVERALRTTRGTLIGVNQRDLVTFEVDQNRAVRVAATIPDGV
                     VKVAESGVRGRSDAEALAAAGYDAVLVGEHLVTSGDPGTALAALKVRRPPHHDR"
     gene            1684..2379
                     /locus_tag="PLP95_09410"
     CDS             1684..2379
                     /locus_tag="PLP95_09410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HPB46057.1"
                     /translation="MWVKICGITNEEDALMAVALGADAIGFVFAPSPRQVSVGVARDI
                     IRRLPPEVEPIGVFRDQDPQFISNAVLEAGLRGVQLHGHETPQLASEITPRPRRLIVA
                     FAAGSPGIERFEEYRADAMLLDAQVPGSGKVFDWDLVDGVPDGARLILAGGLTADNVA
                     AAVERVRPWGVDVSTGVEASPGQKDPVLVSEFIAAVRAAAPAASEVDDPGADPDGAAS
                     TSAPFDWYDDSDL"
     gene            2412..>2855
                     /locus_tag="PLP95_09415"
     CDS             2412..>2855
                     /locus_tag="PLP95_09415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020379093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HPB46058.1"
                     /translation="MSETIRPATLMGDPVDGRFGDFGGMFVPETLVPACQELDRAFRE
                     AWADDGFRAELDHLLASYGGRPSPLTYCSNLSERLGCHLWLKREDLNHTGSHKINNVL
                     GQALLAKRMGKTRLVAETGAGQHGVATATAAALLGMECVVYMGEVD"
BASE COUNT          437 a          925 c         1022 g          471 t
ORIGIN      
        1 ccgatggtgt ggtgggagcg gtaacctcgt cgagtcatgg cgtggcattg tcgatgctca
       61 agcgttcggt cgaaccggac actcgggtaa cggtgggctc ggcggaagcg gtggtaacga
      121 gagttgctgc caaggagtga ggttctgtgg gcggaaacag ttactacgag gtgatcggcg
      181 tgcgtcgtga tgcgggggtt gaggagattc gccacgccta cctgcacgca gcgcgccagg
      241 cccacccgga cgtggccggc ccgtccggtg aagctcggat gcgagagctc aatgaagcct
      301 gggcgacgct gcgcgacgag gagaagcggg cgttctacga cctgtcgatt ccggatctgc
      361 ccgcacccgc ggccggcggt cgtgccggcg acgaatctcg ggcgacacgc gttaggacca
      421 cgccgctgcc gcgcgccggg cgcgtcgggg ccgattatca ctggccgacg cctccacatc
      481 acctcgattt ctccggcctg catcccaacc agcgatccgg tccaccccca accggtcggc
      541 ccggaggggc ccagtttggc ggtggggagc agttcacccc tcccagtccg accccggtcg
      601 accaaaccgt cgggcgcgga acccttgggc gcggaaccct tgggcgcgga acccgcttcg
      661 ctccattggt gctgttcgcc atcggcggcc tcaccggcgc atgcggggca gcgacgtcga
      721 gcccgtcgtt gctggtgatc gcggcggtgg tcgtcgtggt cagtgcgctg ggcgcgctca
      781 tcgtgaggct cgcgtcggcc acgaccgcca aggagcgcgg ggtcggctcg cagaacaggt
      841 aggttcaagg ccgtcatggc tacctacctc gatcgaatcc tcgagttcca ccgcgcccgc
      901 gccgcggccg accgtcgcag cctcgacgag ttgatgcatc gtgccgcagc gatggaggcg
      961 cctcggggat ttcgcgacgc gctcctcggg tcgaccgcag agaccgccga cgccacgctg
     1021 gccgtcatct cggaggtcaa acgccggtcg ccgtcgaagg gggacttgtt cgccgaactc
     1081 gacccggccg ggttggcgca gacctacgag gccggcggcg ccgcgtgtct gtcggtcctg
     1141 accgacgagc accacttcgg agggtccgca aacgaccttg cccaagcccg tgcggcggtg
     1201 tcgttgccgg tgctgcgcaa ggatttcacc gtatgtgcac tcgacgtcgt cgatgcccgg
     1261 ttgatgggag ccgactgcgt gctgttgatc gtcgccgcgc tcagcgacga ggagttggcc
     1321 aacttcgacg atctcgctgc gcagttgggc ctcgacgtgt tggtcgaaac ccacgatgag
     1381 gccgaggtcg aacgggcgct gcgcacgacc cggggaaccc tcatcggtgt gaaccagcgc
     1441 gacctcgtga ccttcgaggt cgaccagaac cgggcggtgc gggtcgcggc caccattccc
     1501 gacggtgtgg tcaaggtcgc cgaatccggt gtgcggggac gctccgatgc cgaggctctc
     1561 gccgctgccg gatatgacgc ggtcctggtg ggggagcacc tggtgaccag cggggatccg
     1621 ggcacagcgc tcgcggcgtt aaaggtgcgg cgaccccctc accacgaccg gtaggttggg
     1681 gccatgtggg tgaagatctg tggcatcacc aacgaggagg acgccttgat ggcggtggcc
     1741 ctcggggccg atgccatcgg gttcgtgttc gctccctcgc ctcgccaggt gagcgtcggc
     1801 gtcgcccgcg acatcatccg gcggctgccg ccggaagtgg agccgatcgg ggtgtttcgc
     1861 gaccaggacc cgcagttcat ctcgaacgcg gtgctcgaag cgggccttcg cggggttcag
     1921 ctccacggcc atgagacccc gcaactggcc tccgagatca ccccgcggcc gcgccgcctc
     1981 atcgtcgcct tcgccgcggg atccccgggg attgagcggt tcgaggagta ccgggcggat
     2041 gcgatgttgc tcgacgccca ggttcccggc agcgggaagg tcttcgattg ggacctggtc
     2101 gacggggtgc ccgacggagc ccggctgatc ctcgccgggg gtttgaccgc cgacaacgtc
     2161 gcagcggcgg tggaacgggt tcgtccgtgg ggggtcgacg tgtcgaccgg ggtcgaggcc
     2221 tcgccggggc agaaggatcc ggtgctggtg agcgagttca tcgccgcagt gcgcgccgct
     2281 gcacccgcgg catcggaggt cgacgatccc ggtgccgacc ccgatggggc cgcgagtacg
     2341 tcggcgccct tcgactggta cgacgactcc gacctatgag tccacgagac agatccaatg
     2401 aggcagacac cgtgagcgaa acgatcagac cggcgacgtt gatgggcgac cccgtcgacg
     2461 gccgattcgg cgacttcggc ggaatgttcg ttcccgaaac ccttgtccca gcgtgccagg
     2521 aactcgatcg ggcgtttcgc gaggcgtggg ccgacgacgg ttttcgcgcg gaactcgatc
     2581 acctgttggc gtcctacggt ggtcgcccgt cgccgctgac ctactgctcg aacctgtcgg
     2641 agcggttggg gtgccatctc tggctcaagc gagaagacct caatcacacc ggctcgcaca
     2701 agatcaacaa cgtgctgggc caggcgttgc tggccaagcg catgggcaag acccgtcttg
     2761 tcgccgagac cggagccggc cagcacgggg tggcgacggc gacggccgcg gccctgttgg
     2821 gcatggagtg cgtcgtctac atgggcgagg tcgac
//
LOCUS       DAOOMA010000349         2853 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_42867_length_2853_cov_5.044317, whole genome shotgun sequence.
ACCESSION   DAOOMA010000349 DAOOMA010000000
VERSION     DAOOMA010000349.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 2853)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2853)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2853
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42867_length_2853_cov_5.044317"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(405..1595)
                     /locus_tag="PLP95_09420"
     CDS             complement(405..1595)
                     /locus_tag="PLP95_09420"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HPB46059.1"
                     /translation="MNGPLSDLRVIEMGQLLAGPFCGQLLADFGADVIKIEAPATGDP
                     MREWGREKAQGASLWWPVVARNKRSVTLNLREAEGQELARQLIGEADILIENFRPGTL
                     EKWGLDPEDLRATRPELIVVRVSGYGQTGPYADRPGYGAIGEAMGGLRAVIGEPDRPP
                     ARAGISIGDTLAAIFACVGTLVAVHHRHNTGRGQIVDSALYEAVLGVMESLIPEYTVA
                     GYVRPRTGSILPNVAPANAYPTSDGEHLISGNQDTVWRRLAAAMGRDDLAEDERFATH
                     NARGQNQELLDGLIAEWTRTLTSARLEEVLNEFGVPNGKIYTAPDMLADAHFAAREAI
                     VTLAHPLLGDFAMHNVVPKLSDTPGSVRWVGPELGQHTDEVFAEVLGLEDGELEALRG
                     RGII"
     gene            1734..2147
                     /locus_tag="PLP95_09425"
     CDS             1734..2147
                     /locus_tag="PLP95_09425"
                     /inference="COORDINATES: protein motif:HMM:NF023125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3293 domain-containing protein"
                     /protein_id="HPB46060.1"
                     /translation="MTSAYGTEAIWDIFDESIVTFTGPDGSSVRYGSDANPYPEPLFG
                     ITAWNPGEERPHEENVAANERLKAHLTQSAIRFVDAEGASPDGTWNEPGYVLIGTDRA
                     TAVAVAREFGQLAIHEMANGVLRVVKSAGNSDTTT"
     gene            2161..>2853
                     /locus_tag="PLP95_09430"
     CDS             2161..>2853
                     /locus_tag="PLP95_09430"
                     /inference="COORDINATES: protein motif:HMM:NF025008.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HPB46061.1"
                     /translation="MATNVLDSTHPTIDPDRPTRTHATIGEEWQIIYSQGGIVMSTML
                     RAATRVLARPDLQLSAMSATFIKPVPMGDVVIDVEVLRSGRNGAQTSIELRDATSAAN
                     GDGPNTVITAVFIAADDNAPTVRSLSCPPELLGTPTPEHVAATAPREQAFLERTDWVL
                     TPEQPDDTNRQLMWFRFHEAPLTDDGTWERSTLVVPADTLGTAIHSAMMAKGDREVFS
                     VSLQITVQFHADA"
BASE COUNT          514 a         1006 c          863 g          470 t
ORIGIN      
        1 tccggtcggc cccaacaccg tcggcaccgg gggtggctgc caaatcgtca ccggggccgt
       61 gtccgggggc gtcaccggtg tcgtgtcggt cacgatcgtg gtcgtatctt caacggcggt
      121 cgatgtcgtt tcgatcgtgg cgacggtggt cgacacgcgc tgaatcgaac cgtcatcgat
      181 gacgtcggca cgagtcgttc cggtgcacgc agcgagcagg gccgaaaccc cgaagacgcc
      241 cgccgtgaga actccatacc gacgcacact gctcaatgcc gaggtgcgac gcataacagc
      301 aattctacgt atgtccgccg acatcttcgg cgcggccggg gcaatttgcc tgcggccggc
      361 agcaccccac gtcggcaccg tcgcggcttg gacacggtgc catctcagat gatgccgcgg
      421 ccgcggagtg cctcgagttc gccgtcttcg aggccgagca cctcggcgaa cacctcgtcg
      481 gtgtgttgac cgagctcagg gcccacccag cgcacggagc cgggggtgtc cgacagcttc
      541 ggcacgacgt tgtgcatcgc gaagtcgccg agcagcgggt gggccagcgt tacgatcgcc
      601 tcgcgcgcgg cgaaatgagc gtccgccaac atgtcggggg cggtgtagat cttgccgttc
      661 ggcaccccga actcgttgag cacttcctca agccgtgccg aggtcagggt gcgggtccac
      721 tccgcgatca atccgtcgag cagttcctgg ttctgtccgc gggcgttgtg cgtggcgaaa
      781 cgctcgtcct cggcgagatc atcacgaccc atcgccgctg ccagccggcg ccatacggtg
      841 tcctgattgc cgctgatcag atgctcaccg tccgaagtcg gataggcgtt ggcgggagcg
      901 acgttcggca gaatcgaccc ggttcgcggt cgcacgtacc cggcgaccgt gtactcggga
      961 atcagcgact ccatcacccc gagcaccgcc tcgtacagcg ctgaatccac gatctgacct
     1021 cgaccggtgt tgtggcgatg atggaccgcc accagcgtcc cgacgcacgc gaagatcgcc
     1081 gcaagcgtgt cgccgatcga gatgccggca cgagccggag ggcggtcggg ttcgccgatg
     1141 accgctcgaa gcccgcccat cgcctcgccg atcgcaccat aaccggggcg atcggcatac
     1201 ggtccggtct ggccgtatcc cgaaacacga acgacgatga gttcgggtct cgtcgcccgc
     1261 agatcctccg gatccagccc ccacttctcc agcgttcccg gcctgaagtt ctcgatcagg
     1321 atgtcggcct cgccgatcaa ctgcctcgca agctcctgcc cctcggcctc gcgcagattc
     1381 agggtgaccg agcgcttgtt gcgtgccacg acgggccacc acaacgacgc tccttgcgcc
     1441 ttttcgcgcc cccactctcg catcggatca ccggttgccg gggcctcgat cttgatgacg
     1501 tcggcaccga agtccgcaag gagttgtccg cagaagggcc cggcgagcag ctgtcccatt
     1561 tcgatgaccc gaagatcgct cagcggtccg ttcacccgtg tgtcctccga tgccgtgctc
     1621 cggcctgacg ctcaacgcga cgaccaacga tacgtcgcgc gacatgacac agcagggcgc
     1681 aacgaaacga caccgctccc acacggccgt agtcggtctg ctgaactggc gccatgacga
     1741 gcgcgtacgg aaccgaggcc atctgggaca tcttcgacga gtcgatcgtc acgttcaccg
     1801 gccccgacgg atcgtcggtc cgctacggct ccgatgccaa cccgtacccc gagccgctct
     1861 tcggaatcac cgcctggaat ccgggcgagg aacgaccaca cgaagaaaac gtcgcagcaa
     1921 atgagcgcct caaagcccac ctcacacaaa gcgccattcg attcgtcgac gccgagggcg
     1981 cctcgcccga tggcacctgg aacgagccgg gctacgtgct gatcggcacc gaccgggcga
     2041 ccgccgtcgc ggtcgcccgt gaattcgggc agctcgcgat ccacgagatg gccaacgggg
     2101 tgcttcgcgt ggtgaaatcc gccggcaact ccgacaccac gacgtaggac cccgcatccc
     2161 atggcaacga acgttctcga cagcactcac cccacgatcg accccgaccg gccgacccga
     2221 acccatgcca cgatcggcga ggaatggcag atcatctact cacagggcgg gatcgtgatg
     2281 tcgaccatgc ttcgtgccgc aacacgggtt ctcgctcggc ccgaccttca gctctccgcc
     2341 atgtccgcga cgttcatcaa gccggtaccg atgggcgacg tggtgatcga cgtcgaggtt
     2401 ctgcgcagcg ggcgcaatgg cgcccagaca tcgattgagc tgcgcgacgc aacaagtgcc
     2461 gccaacggtg atggtcccaa caccgtgatc accgccgtct tcatcgccgc cgacgacaac
     2521 gcgccaaccg tgcgctcgct cagttgccct cccgagcttc tcggcacgcc aacccccgaa
     2581 cacgtcgccg ccacggcgcc acgagaacag gcgtttctgg agcgcaccga ctgggtcctc
     2641 acccccgaac agcccgatga tacgaaccgg caattgatgt ggtttcgctt tcacgaggcc
     2701 ccgctcaccg acgacggaac gtgggagcgt tccaccctcg tcgttccggc cgacaccctc
     2761 ggcaccgcca ttcactcggc gatgatggcg aagggcgacc gcgaggtgtt ttcggtgtcg
     2821 ttgcagatca ccgtgcagtt ccacgcagat gcc
//
LOCUS       DAOOMA010000350         2843 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_43113_length_2843_cov_3.724892, whole genome shotgun sequence.
ACCESSION   DAOOMA010000350 DAOOMA010000000
VERSION     DAOOMA010000350.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 2843)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2843)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2843
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43113_length_2843_cov_3.724892"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1379)
                     /locus_tag="PLP95_09435"
     CDS             complement(<1..1379)
                     /locus_tag="PLP95_09435"
                     /inference="COORDINATES: protein motif:HMM:NF013173.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-6-phosphate synthase"
                     /protein_id="HPB46062.1"
                     /translation="MTNRLPVRRVRDREARPRDGIADVAGQHWEASPGGLVAALGPVF
                     DRRPGEATWVGWSGTHGEELDPFEVDGLTLVPVDLTRGEVQLYYEGFCNATIWPLYHD
                     SIIRSEYHRTWWDSYLAINRRFASAAIEAVAPGGVVWVHDYQLQLAPSMIRSVRNDVR
                     IGFFLHIPFPPVELFLQLPWRRQILLGLAGADVVGFQTAGGAANFRALIERLGIGTPD
                     GDDVIVDGRRVEVRVFPIGVDAQRIARTAADPAVQARAVEIRESLGNPDTLVLGIDRL
                     DYTKGIARRMQAFAELHQEERLEMPRHVMIQVAEPTRESVPGYAEFRERVDALVGDIN
                     GNLGLVGRPAINYIHRSQDFEEVVALYLAADVMLVTPVRDGMNLVSKEFVAARPDDTG
                     VLVLSEFTGAADALSEALLVNPYDTDGLKWAIDRAVHLPEDEMRRRMAALRVEVTTND
                     VYRWADDWM"
     gene            1512..2171
                     /locus_tag="PLP95_09440"
     CDS             1512..2171
                     /locus_tag="PLP95_09440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HPB46063.1"
                     /translation="MSTGEWHPAFEEYCETIYELAEEGVRVVQARISERLDVSRPSVS
                     GMVKRLSADGLVELNGPEIALTDSGLELAEQVVRRHRLAERFLCDVLGLPWAQAHHEA
                     GKWEHVIGDDVEGALIRVLGEPTTCPHGNPIPGTAYEPQEVRSLASLSAGTDFVVSRI
                     PESLEFEPGVLEFLEQCHIVPGATGTVSATAPDGTVTLQIDDRTFGVGAFAADRILVA
                     A"
     gene            complement(2266..>2843)
                     /locus_tag="PLP95_09445"
     CDS             complement(2266..>2843)
                     /locus_tag="PLP95_09445"
                     /inference="COORDINATES: protein motif:HMM:NF020252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HPB46064.1"
                     /translation="AVGLAAAWLLSLAAMAAAGVTASGVKTPAEFWAHAASTAAGASA
                     VASRFGIPTADCFAAGLLHDVGMVFLQQIDPDRHNALLVEHGSHGAGLVEAERRVFGM
                     SHAEAAAHVLRTWKFPQRFVDAVAGHHDPEADPFAQAVAVGEALAIAGVGDDPVAEAV
                     LERSGIEGEDRATLIEYTVQRAREVLASLPH"
BASE COUNT          498 a          975 c          923 g          447 t
ORIGIN      
        1 ttcatccagt cgtctgccca gcggtacaca tcgttggtcg tcacctccac ccgcaacgcc
       61 gccattcggc gccgcatctc gtcctcgggc agatgaacgg cgcgatcgat cgcccacttg
      121 aggccgtcgg tgtcgtacgg attgaccaac aacgcttcgc tcagcgcatc ggcagccccg
      181 gtgaattcgc tgagcacgag caccccggtg tcgtcgggtc gagccgcgac gaattccttc
      241 gacacgaggt tcatgccatc tcgaaccggg gtcaccaaca tgacatcggc ggcaaggtac
      301 agcgctacga cctcctcgaa gtcctgactt cggtggatgt aattgatcgc cggacgtccg
      361 acgaggccga ggttgccgtt gatgtcgccc acgagcgcat ccacccgctc gcgaaactcc
      421 gcatagcccg gcaccgactc cctggtcggc tcggccacct gaatcatgac gtgacgcggc
      481 atctcgaggc gctcctcctg gtgcaactct gcaaatgcct gcattcgccg ggcgatgcct
      541 ttggtgtaat cgaggcgatc gatgcccaac acgagcgtat ccggattgcc gagcgactcg
      601 cggatctcca cggcgcgcgc ctgaacagcc gggtcggcgg cggtccgcgc tattcgctgg
      661 gcgtcgaccc cgatcggaaa cacccgcacc tcgacccgcc gaccgtccac gatgacgtcg
      721 tcgccgtcgg gcgttccgat cccgagacgc tcgatgagcg ctcggaagtt cgccgcgccg
      781 ccggccgtct ggaacccgac cacatcggca cccgcgaggc cgagcagaat ctggcgccgc
      841 catggcaact gcaggaacaa ctcgaccggg ggaaacggga tgtgcaggaa gaatccgatt
      901 cgcacgtcgt tgcggactga ccgaatcatc gacggggcca actgcaattg gtagtcgtga
      961 acccacacga cgccgccggg ggcgaccgcc tcgatcgctg cggaggcgaa gcgccggttg
     1021 atggccagat aggagtccca ccaggtgcgg tggtattccg aacggatgat ggagtcgtga
     1081 tagagcggcc agatcgtggc gttgcagaaa ccctcgtagt acagctgcac ctcgccgcgg
     1141 gtgagatcga ccgggaccag ggtcaggccg tccacctcga aggggtcgag ttcttcgccg
     1201 tgggtgcccg accatcccac ccaggtcgcc tcgcccgggc ggcgatcgaa caccggaccg
     1261 agcgcggcga cgaggccacc gggactggct tcccagtgct gacctgccac gtcggcgatt
     1321 ccatcgcgcg gcctcgcttc gcgatctcgc acgcgcctca ccggaagacg attggtcatc
     1381 accaccaatg acgaatcccg accgatccca caaggtgctg ttcgctgcgc tgtttggccg
     1441 accacatcga caacgctagc gatgaggtcc ggacaaaacg cggcacccca atcgccacta
     1501 gggtggcgcc catgtcgacc ggcgaatggc accccgcatt cgaggaatac tgcgagacca
     1561 tctatgaact cgcagaagag ggcgtacgtg ttgtccaagc gcgtatttcc gagcgcctcg
     1621 atgtttcccg gccgtcggtg tcgggaatgg tcaaacgcct gagtgcagat gggttggtcg
     1681 aactcaacgg ccccgagatc gccctgaccg attccggact cgaactggcc gaacaggtcg
     1741 tacggcgcca tcggctcgcc gagcggttcc tgtgcgacgt cctcgggctc ccgtgggctc
     1801 aggctcacca cgaggcgggc aagtgggaac acgtcatcgg cgacgacgtt gagggcgcac
     1861 tcatccgcgt gctcggggaa ccgacgacgt gtccgcacgg caacccgatt cctggcacgg
     1921 cctacgaacc tcaggaggtg cggagcctcg cgtcgctgag cgcaggcacc gacttcgtcg
     1981 tgtcacggat tcccgagtcg ctcgagttcg agccgggcgt gctcgagttc ctggagcagt
     2041 gccatatcgt tccgggcgct acgggcaccg tgtcggccac ggcacccgat ggcaccgtca
     2101 cgctgcagat cgacgacagg accttcggag tcggcgcatt cgctgccgac cggatcctcg
     2161 tcgccgcctg agcagcagct gaccgtcggc gggtcagtgc ggcagggcgg gtcagtgcgg
     2221 cagggcgggt cagtgcggca gggcgggtca gtgcggcacg gcgggtcagt gcggcaggga
     2281 ggccagcacc tcgcgggccc gttgcacggt gtattcgatc agcgtcgcac ggtcctcgcc
     2341 ctcaattcct gagcgttcga gcaccgcctc cgcgacgggg tcgtctccaa caccggcgat
     2401 ggcgagcgct tcgccgaccg cgaccgcctg ggcgaacggg tcggcctccg ggtcgtgatg
     2461 gccggcgacc gcatcgacga atcgctgcgg gaacttccag gtgcgcagga cgtgggcggc
     2521 agcctcggca tgcgacatgc cgaacacccg ccgttcggcc tccacgagcc cggcaccgtg
     2581 cgagccgtgt tcgacgagca gggcgttgtg tcggtcagga tcgatctgct ggaggaacac
     2641 cattccgacg tcgtgcagca gtccggccgc gaagcagtcc gctgtcggga tgccgaatcg
     2701 agacgccacc gcagacgccc cggcggccgt cgacgccgca tgggcccaga actccgccgg
     2761 agtcttcacc ccactcgccg tcaccccagc cgcggccatc gcggcaagcg acagcagcca
     2821 cgcggccgcc aggccgacgg cca
//
LOCUS       DAOOMA010000351         2836 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_43262_length_2836_cov_4.058252, whole genome shotgun sequence.
ACCESSION   DAOOMA010000351 DAOOMA010000000
VERSION     DAOOMA010000351.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 2836)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2836)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2836
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43262_length_2836_cov_4.058252"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..774
                     /locus_tag="PLP95_09450"
     CDS             <1..774
                     /locus_tag="PLP95_09450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012222737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sir2 family NAD-dependent protein deacetylase"
                     /protein_id="HPB46065.1"
                     /translation="ETTARADPIEQATHLVDHAERVLVLSGAGISTDSGIPDFRGPKG
                     VWTRNPGAEKLSSIEHYLADPEVRVRAWQARIDNPAWGAIPNRGHDAVRHLERRKKLL
                     LLVTQNVDGLHHDSGVDPDRIVEIHGTMREVVCLSCGAREPAGPTLERIRSGDRDPHC
                     RMCGDGILKSATISFGQNLIERDLDRSFAAAMDADLVIAVGTNLSVYPVAGIVPAAKD
                     AGARVVIVNGTETDMDPLADVIVRGSISEMLPKICGGPA"
     gene            complement(787..1713)
                     /locus_tag="PLP95_09455"
     CDS             complement(787..1713)
                     /locus_tag="PLP95_09455"
                     /EC_number="3.5.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF017864.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HPB46066.1"
                     /translation="MLNVEVLRGDLVESVHRIRACAVDRAGVVIGEAKPADADWPIFM
                     RSAAKPFQAAPAAAAGVIEELGLTDEHLAVACASHDGSAYPVGLVREILSAARLDEFA
                     VHTGDDGQGGLVRHQCSGNHALALAFCVVAGWPVDGYLELDHPVQRAMNESVAAACGV
                     SPVLAADNCGMPTHRVPLSAVARAFGRLAGDDAGLDGLGRVVEAMRQHPYTVRQRGQI
                     DSELIAASGGAVVAKVAAEAGVGVGTSDGVGLAVRVEDGSPRAWGVATMAVLRRWIPN
                     APANPVLDSLAAPVLFDGAHRPVGSMRAVWRD"
     gene            complement(1775..2629)
                     /locus_tag="PLP95_09460"
     CDS             complement(1775..2629)
                     /locus_tag="PLP95_09460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017128 - phospholipid scramblase activity
                     [Evidence IEA];
                     GO_process: GO:0017121 - plasma membrane phospholipid
                     scrambling [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid scramblase-related protein"
                     /protein_id="HPB46067.1"
                     /translation="MSPTAPADWYPDPHGRHELRWFDGSGWTDHVCSNGQQGIDPQPV
                     AAVPFAEVNAQGGATVPRADLSAERFEKSLAKVGLDAGDAGGCTGHLMTESVLVVSQK
                     AKFVELTNEYAVLNQAGAQVGAVREIGQSTARKVLRFATNVDQFLTHRYEVVDMAGNI
                     IFSMVRPAKFLKSKVQVSDAMGTPIGEIVQENALGKIHFGLNVEGQRLGSINAENWRA
                     WNFNIQDAGGAEIARITKTFSGLARATFTSADNYVVQIDPTLQGPLRVMAVAAAVSVD
                     TALKQDSR"
BASE COUNT          486 a         1017 c          869 g          464 t
ORIGIN      
        1 gagacgaccg cccgcgctga cccgatcgaa caggccacgc accttgtcga ccatgcagag
       61 cgggtcctgg tcctcagcgg cgctggcatc tccaccgact cgggaatccc cgactttcgg
      121 gggccgaaag gcgtgtggac ccgcaatccc ggtgccgaga agctgtcctc gatcgagcac
      181 taccttgccg atcccgaagt gcgtgtccgc gcctggcagg cgcggatcga caatcccgcg
      241 tggggagcga tccccaaccg cggccacgac gcagtgcgcc acctcgaacg ccgcaagaaa
      301 ctgctgttgc tcgtcaccca gaacgtcgac ggactccacc acgactccgg ggtcgacccc
      361 gatcgcatcg tcgagatcca tgggaccatg cgcgaggtgg tttgcttgtc gtgcggggcc
      421 cgcgagcccg ccgggccgac cctcgagcgc atccgttccg gcgaccggga cccacactgt
      481 cgcatgtgcg gcgatgggat cttgaagtcc gcgacgatca gcttcgggca gaacctcatc
      541 gaacgcgacc tcgatcgcag ctttgccgca gcgatggacg cggacctcgt catcgcggtc
      601 gggaccaacc tgtcggttta tccggtcgcc ggcatcgttc ccgccgccaa ggacgccggg
      661 gcgcgcgtcg tcattgtgaa cgggaccgag accgatatgg acccgctcgc cgacgtgatc
      721 gtgcgtggat cgatcagcga gatgctcccc aagatctgcg gcggccccgc ctgatcgtca
      781 gcgggctcag tcgcgccaca cggctcgcat cgacccgacc ggccggtgag cgccgtcgaa
      841 gagcacggga gcggccaacg aatcgagcac cgggttcgct ggagcattcg gaatccaacg
      901 tcgcagcacc gccatggttg ccacccccca cgcccgaggc gatccgtcct cgacccgcac
      961 cgcaaggccg actccatcgc tggtgccgac ccccactccc gcctcggccg ccaccttggc
     1021 gacgaccgct cccccacttg ccgcgatgag ttcgctgtcg atctgcccac gctgtcgcac
     1081 ggtgtaggga tgctgacgca tcgcttcgac gacacgaccc aggccatcga gtccggcatc
     1141 gtcgccagcg aggcgaccaa acgcccgggc cactgcggaa agcggcacgc gatgggtggg
     1201 catgccgcag ttgtcggctg cgagcacggg cgacacgcca cacgccgcgg cgacggactc
     1261 attcatcgcc cgttgcacgg ggtgatccaa ctcgaggtac ccgtccaccg gccacccggc
     1321 cacgacgcag aacgccagtg ccaacgcatg gtttcctgaa cattgatgcc gcaccaatcc
     1381 gccttgaccg tcgtcgccgg tatgcaccgc gaactcgtcg agacgggcgg ctgaaagaat
     1441 ttcgcgcacc aacccgacgg gataggccga gccgtcatgc gatgcacagg cgaccgccaa
     1501 gtgttcgtcg gtcagcccga gttcctcgat gacaccggcc gccgcggccg gggccgcctg
     1561 aaacggcttg gccgccgaac gcatgaagat cggccaatcg gcatcggccg gcttcgcctc
     1621 gccgatcacc acaccagccc tgtcgaccgc gcacgctcgg attcgatgca cgctctcgac
     1681 caggtcgccc cgcaacacct ccacgttcaa catagggccg acgctaccgg gcaccgctac
     1741 cgggcaccgc tgccggaacg acgcttgccg ggcactatct gctgtcttgc ttgagggccg
     1801 tgtcgacgct gaccgctgcg gccaccgcca tcacgcgcaa cggcccctgc aacgtcgggt
     1861 cgatctgaac gacgtagttg tccgccgagg tgaacgtggc tctcgccagc cccgagaagg
     1921 tcttggtgat ccgggcgatc tcggcgccgc cggcgtcctg gatgttgaaa ttccatgccc
     1981 gccagttctc cgcattgatc gacccgagac gttggccctc gacattgagc ccaaagtgga
     2041 tcttgccgag cgcattctcc tgaacgatct cgccgatcgg cgttcccatc gcgtcgctca
     2101 cctgcacctt cgacttcagg aacttcgctg gtcgcaccat cgaaaagatg atgttgccgg
     2161 ccatgtcgac gacctcatag cggtgggtga ggaactgatc gacgttggtc gcaaaccgca
     2221 gcacctttcg agcggtcgac tggccgatct cgcgaaccgc tcccacctgc gctcccgcct
     2281 ggttcaacac ggcatattcg ttggtgagct cgacgaactt cgccttctgg ctgacgacga
     2341 gaacggattc ggtcatcagg tgtccggtgc atccgcccgc gtcaccggca tcgaggccga
     2401 ccttcgccaa cgacttttca aaccgttccg ccgacagatc cgcccgcgga accgtcgcac
     2461 cgccttgagc gttcacttct gcgaacggaa cggcagcgac cggctggggg tcgatccctt
     2521 gttggccgtt cgagcacaca tggtcggtcc accccgaacc gtcgaaccat cgcagttcgt
     2581 ggcgtccgtg cggatcgggg taccagtcgg caggggctgt cgggctcatc acgtctccag
     2641 ataggtcgac ctcccaccgt atcggcagcg cccccgcgtt ccacgcgacg gtgccgcgcc
     2701 taagggcgcg gcaccgtcgc ggtcaccagc ggtttggtga gccgggctca ggttgcgggg
     2761 acctcgggcg cagctggggc cgccgaagcg atgaacagct cctgttcgga gttcttcgct
     2821 ccgctgccgg catagg
//
LOCUS       DAOOMA010000352         2822 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_43571_length_2822_cov_3.780990, whole genome shotgun sequence.
ACCESSION   DAOOMA010000352 DAOOMA010000000
VERSION     DAOOMA010000352.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 2822)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2822)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2822
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43571_length_2822_cov_3.780990"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..301)
                     /locus_tag="PLP95_09465"
     CDS             complement(<1..301)
                     /locus_tag="PLP95_09465"
                     /inference="COORDINATES: protein motif:HMM:NF013584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HPB46068.1"
                     /translation="MSAGRPQTALEIAASIRSGERSAAEVIEDALASIAATEPQVGAY
                     NLVLDEAARAAAAEIDRRIAAGQEVGALAGVPIAIKDNICTRGVETTCSSRILKGW"
     gene            complement(298..600)
                     /gene="gatC"
                     /locus_tag="PLP95_09470"
     CDS             complement(298..600)
                     /gene="gatC"
                     /locus_tag="PLP95_09470"
                     /inference="COORDINATES: protein motif:HMM:TIGR00135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HPB46069.1"
                     /translation="MPERISRDDVAQVAKLARLVLSDEDLVRATAQLGDILDHARDMA
                     ALDLDGVEPTAHPYPLVNVFRDDVATASLDREEVLASAPAAEDGMFRVPPVIGEEP"
     gene            699..1103
                     /locus_tag="PLP95_09475"
     CDS             699..1103
                     /locus_tag="PLP95_09475"
                     /inference="COORDINATES: protein motif:HMM:NF014132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 sterol-binding domain-containing protein"
                     /protein_id="HPB46070.1"
                     /translation="MAKFQFLTPEWVEAAKAIRADYEGTASPHPVRANLIITEVPFGP
                     GEEKAFMDGSGEAMEIGLGELDSADLTMTVDYLTAKAIIVEGNPQAGMQAFMAGKIKV
                     VGDMTKMMGLAGGAVEDPNAAEVAQRIQDITE"
     gene            complement(1234..2622)
                     /locus_tag="PLP95_09480"
     CDS             complement(1234..2622)
                     /locus_tag="PLP95_09480"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HPB46071.1"
                     /translation="MTNRRAAGPWLVTWSVLTGAFAIGLNFTILTVSRPSIAEDLNTS
                     VASLVWLVSGPIVANALVAATGGKLGDILGHRRVYLASFAGMTVFTVLCAFAWSPASL
                     ITFRIVSAMLGAAISPCAMAIINLMFAPHERAKALGYWALVGAGSPMIGLVIGGPLVE
                     AFGWRSIFVIQIPVLVGALAVCWRVLPHTERGERTRFDVAGNVALAAAVLALMLLAER
                     TPKWGWSDARTIGIAVAAVAFARAFVVIERRVEHPLIPIRYFRRRSFTSSVIAATFAQ
                     FGYMGGFILLPVLLDEVGNWSDTRVSLTSTPRPFAFAIAGMVAGRLFATVEPRTLTLV
                     GTSMISVSLVMIAFAVDGLPLWLIVASALVSGWGMGTVQPVSSTVVANSVSNQDLGVA
                     GATVQMAIQVATSLGMNIFDAMHTALGATNGTLGGFERSYQIGALISAGGIAAALWIP
                     RRAHLTHDPIGH"
BASE COUNT          534 a          963 c          930 g          395 t
ORIGIN      
        1 tccaaccctt gaggatccgc gaactgcagg tggtctccac ccctcgggtg cagatgttgt
       61 ccttgatcgc gatcggcacc cccgccaacg caccgacctc ctggcctgca gcgatacggc
      121 ggtcgatctc ggcggccgcg gcccgagccg cctcgtcgag caccaggttg taggcaccga
      181 cctgcggttc ggtcgcagcg atggatgcca gcgcgtcctc gatcacctcg gcggccgacc
      241 gctcgcccga gcggatcgag gcggcgatct cgagcgcggt ctgtggacgt ccggcgctca
      301 cggctcctcc ccgatcaccg gaggaactcg aaacatgccg tcctcggcgg ccggcgccga
      361 cgcgagcacc tcttcacggt cgagggaggc cgtcgcaacg tcgtcgcgaa acacgttgac
      421 gagcggatag ggatgcgcgg tgggttcgac cccgtcgaga tcgagcgccg ccatatcgcg
      481 agcgtgatcc aaaatgtcgc cgagctgagc ggtggcgcgc accaggtcct cgtcgctgag
      541 aaccaggcgc gccagcttcg ccacctgtgc gacatcgtcg cgggaaatgc gttctggcat
      601 aggacgacag tctaggacgg cgcctggatc cggcccgcgg caccgcagcg gtctacagtg
      661 acgccgttca caccccgcga ggagagagaa gcccgacgat ggcgaagttc cagttcctga
      721 cccccgaatg ggtcgaagca gccaaagcga tccgcgccga ctatgagggc acggcatcac
      781 cgcatccggt gagggccaac ctgatcatca ccgaggtccc cttcggcccc ggcgaggaga
      841 aggcattcat ggacggcagc ggagaggcga tggagatcgg cctcggcgaa ctcgacagcg
      901 ccgacctcac gatgacggtg gactatctca ccgcgaaggc gatcatcgtc gagggcaacc
      961 cgcaggccgg catgcaggcg ttcatggccg gcaagatcaa ggtcgtcggc gatatgacca
     1021 agatgatggg actcgccggc ggcgccgtcg aagaccccaa cgccgccgag gtcgcccagc
     1081 gcatccagga catcaccgaa tagcaccgcg tcgcaccgag taagcgcacg ccgcagaact
     1141 ctgggcgcgc tagcaggtca catccgaccc gctagcgcgc ccagagttga ttttcgacgc
     1201 gtacgcgagc cgtgctcggt acccgatcgt ggctcagtgc ccgatcgggt catgggtgag
     1261 gtgggcgcgc cgcgggatcc acagcgcggc cgcgattccg cccgccgaga tgagcgcgcc
     1321 gatctgatac gagcgttcga acccgccgag cgttccgttc gtcgctccga gtgcggtgtg
     1381 catcgcatcg aagatgttca tgccgagcga cgtcgccacc tggatcgcca tctgaaccgt
     1441 ggctccggcc accccgaggt cctggttcga caccgagttt gcgacgaccg tcgagctcac
     1501 cggctgcacc gtgcccatcc cccagcccga caccaacgcc gaggccacga tgagccacag
     1561 cggcaacccg tcgacggcga aggcgatcat caccagcgac acgctgatca tcgaggttcc
     1621 gaccagggtc agcgtgcgag gttcgaccgt ggcgaagagc cgaccggcca ccatcccggc
     1681 gatcgcgaac gcgaacggcc gcggtgtgct cgtgagcgag acccgggtgt cgctccagtt
     1741 gcccacctcg tcgagcaaca cgggcaacaa gatgaacccg cccatgtagc cgaattgggc
     1801 gaaggtcgcg gcgatcaccg acgaggtgaa cgacctgcgc ctgaagtagc ggatggggat
     1861 cagtggatgc tcgaccctgc gctcgatgac gacaaaggcc cgggcgaacg ccaccgcagc
     1921 gaccgcgatg ccgatcgtgc gggcatcgga ccagccccac ttcggtgtgc gctcggccaa
     1981 caacatgagc gccagcaccg cggcggcgag cgccacgttg ccggcaacgt cgaaccgggt
     2041 gcgctccccc cgttcggtgt ggggcagaac ccgccagcac actgcgagcg cacccacgag
     2101 caccggaatc tggatgacga agatcgagcg ccatccgaac gcttcgacga gcgggccgcc
     2161 gatcaccagg ccgatcatcg gcgatccggc gcccacgaga gcccagtacc cgagcgcctt
     2221 ggcgcgctcg tgcggggcga acatcaggtt gatgatcgcc atcgcacacg ggctgatcgc
     2281 ggcgccgagc atcgccgaga cgatcctgaa tgtgatgagc gaggcgggcg accaggcgaa
     2341 tgcgcacaaa acagtgaaca ccgtcattcc cgcaaagctc gcgaggtaca cccggcggtg
     2401 gcccaggatg tcgccgagct ttccaccggt ggccgccacc aacgcgttcg ccacgatggg
     2461 acccgacacc agccacacga gcgacgcgac actcgtgttg aggtcctcgg cgatcgaggg
     2521 tcggctcacg gtgagaatgg tgaagttcag cccgatggcg aatgcgccgg tgagcaccga
     2581 ccacgtcacc agccacggac ctgcggcgcg ccgattcgtc atgggatatc gccggtcatg
     2641 gagcaccatc gtcgcacagt tgcgaagcca ccggtgcggc cgcgtccgcc aacgtttcgg
     2701 ggtgacatgt agccggttgg ggtggccgcc tcgtcgcggc ggctggggaa caccacgtgg
     2761 gcagaccacc ccaacccgct acatgccgta gggaaaatgt agggcggcgc ttgtcgcggg
     2821 tg
//
LOCUS       DAOOMA010000353         2821 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_43593_length_2821_cov_4.054230, whole genome shotgun sequence.
ACCESSION   DAOOMA010000353 DAOOMA010000000
VERSION     DAOOMA010000353.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 2821)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2821)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2821
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43593_length_2821_cov_4.054230"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..491
                     /locus_tag="PLP95_09485"
     CDS             <1..491
                     /locus_tag="PLP95_09485"
                     /inference="COORDINATES: protein motif:HMM:NF013907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB46072.1"
                     /translation="HGLRGEVSVRLISDRLERVAPGSQLQTNRGVLTVVSSRPHQNRW
                     IVQFEGVAGRTAAEELQGTVLSAEAIDDPSALFVHDLIGAHVVDADGIDRGVVRAVQA
                     NPASDLLVLDGDVLVPMVFIVGDVERADGGDGGDGADGGDGGGSERRVVRVEVPDGLF
                     DL"
     gene            501..1253
                     /gene="trmD"
                     /locus_tag="PLP95_09490"
     CDS             501..1253
                     /gene="trmD"
                     /locus_tag="PLP95_09490"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015798900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N(1))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HPB46073.1"
                     /translation="MRIDVFSIFPEMIRGFAEHSLLGKARGNGLVDLRAYDLRDHAPD
                     RHKTTDDAPFGGGAGMVMTPGPIFAAVEAADAPRPLLYLSPSGRRFDQEMAEELAGGE
                     GFSLLCGRYEGVDQRVIDHLCDGEVSIGDYVLNGGEVAALVMIEAVTRLVPGVMGNAE
                     SGLDESFSSGLLEYPHYTRPAEFHGWSVPAVLRSGDHGRIERWRRAEALAKTLRRRPD
                     LIAGAALSDQDRALLADHGYDLLGLPTGESAS"
     gene            1389..1730
                     /gene="rplS"
                     /locus_tag="PLP95_09495"
     CDS             1389..1730
                     /gene="rplS"
                     /locus_tag="PLP95_09495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015798899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HPB46074.1"
                     /translation="MKPTDVVDQKSMRDDVPDFAPGDTLKVHVRVVEGTRERVQVFQG
                     AVIRRQGGGINETFTVRKVSFGVGVERTFPVHSPTIAKIEVFTRGDVRRAKLYYLRDR
                     VGKAAKIKEKR"
     gene            1876..2247
                     /locus_tag="PLP95_09500"
     CDS             1876..2247
                     /locus_tag="PLP95_09500"
                     /inference="COORDINATES: protein motif:HMM:NF009154.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HPB46075.1"
                     /translation="MSTQRSTIGRDGETVAANWYLRAGYEIEARNWHCREGELDIVAH
                     RIDERGVACVVVCEVKTRSSARFGSAAEAVTRSKQYKIRAATVRYLAQRDGGPCSIRF
                     DVAVVDRSPHGYGVRVIEGAF"
     gene            2473..>2821
                     /locus_tag="PLP95_09505"
     CDS             2473..>2821
                     /locus_tag="PLP95_09505"
                     /inference="COORDINATES: protein motif:HMM:NF016240.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF480 domain-containing protein"
                     /protein_id="HPB46076.1"
                     /translation="MSDLSTPDDPAAENGAGGGDETAGMLTEEQARVLGSLIEKSRTT
                     PDDYPLTANALMRACNQSTSRDPVVNYDQRQVERVVAELKAMGLVRFVHMATGRAVTK
                     YRHVVDEAWGLDPQ"
BASE COUNT          471 a          868 c          997 g          485 t
ORIGIN      
        1 cccacgggct gcggggtgag gtgagcgttc gactgatctc cgatcggctc gaacgtgtcg
       61 ctccaggatc acaattgcag acgaaccgcg gtgtgctcac cgtggtttcg tcgcgcccac
      121 accagaatcg ttggatcgtc cagttcgagg gggttgcggg ccgcactgca gccgaggaac
      181 tgcagggcac cgtgttgtcg gccgaggcga tcgacgatcc gagtgcgctg ttcgtccacg
      241 acctgatcgg cgcccacgtg gtcgacgccg acggtatcga ccggggcgtg gttcgtgcag
      301 tccaagcgaa cccggcgagc gacctgttgg tgctcgacgg cgacgtattg gttccgatgg
      361 tcttcatcgt cggcgacgtc gaacgggccg acggcggtga tggcggtgat ggcgccgatg
      421 gtggcgatgg cggtggtagt gagcgccgag tcgtgcgcgt cgaggttccc gacggtcttt
      481 tcgacctgta ggcatccgtg atgcgcatcg atgtgttcag catctttccc gagatgatcc
      541 gcgggttcgc cgaacactcg ttgctcggca aagcccgcgg caacggtctc gtcgacctgc
      601 gggcctacga cctgcgcgat cacgccccag accgtcacaa gaccaccgac gatgcgccgt
      661 tcggcggagg tgccgggatg gtgatgaccc cggggccgat cttcgctgcg gtggaggcgg
      721 ccgacgcgcc ccgtccgctg ctgtatctgt ccccgtcggg gcggcgcttc gaccaggaga
      781 tggccgagga attggccgga ggcgagggct tctcgctgct gtgtgggcga tacgagggcg
      841 tggatcagcg cgtgatcgac cacctgtgcg acggtgaggt gtcgatcggc gactacgtcc
      901 tcaacggtgg cgaggtggcg gcgctggtga tgatcgaggc ggtgacgcga ctcgtgcccg
      961 gcgtgatggg caatgccgag tcgggccttg acgagtcgtt ttcctcgggg ctcttggagt
     1021 atccccacta caccaggccc gcggagtttc acgggtggtc ggtgccggcg gtgttgagaa
     1081 gcggcgatca cggccgcatc gagcggtggc gacgggctga ggcgctcgcc aagacgttgc
     1141 gcaggcggcc cgatctgatc gccggcgccg cgttgagcga tcaggaccgc gcgttgttgg
     1201 ccgaccacgg ctatgatctc ctcggccttc ccacgggtga atccgcctcc tgaacgcgat
     1261 gctctgcgtc gcttcgtcgg ggtcggggct cggtcttggt gccgagagcc gtggacgagt
     1321 aggctctcat ccctgtgcgc ccgcaggcgc gccccagttc gaaacatccc cgcaggagct
     1381 accgcgccat gaagccgacc gacgttgtcg accagaagag catgcgagac gacgtacccg
     1441 actttgcccc gggcgacacc ctgaaggtgc acgtgcgagt cgtcgaaggc actcgtgagc
     1501 gtgtccaggt gttccagggc gccgtcatcc gtcgtcaagg tggcggaatc aacgagacct
     1561 tcaccgttcg caaggtgagc ttcggcgtgg gcgtcgagcg aacgtttccg gttcactcgc
     1621 cgacgatcgc gaagatcgag gtcttcaccc gcggcgatgt gcgacgggcg aagctctact
     1681 acctgcgcga ccgcgtcggt aaggcggcca agatcaagga aaagcgctaa gcgcaaccct
     1741 gtggccacct cacaacaggt ggcgacgtca cccgcctcgg agatggcgat ggcgtgataa
     1801 cgacttctcc tcccgtgttg ttccccggcc aggtccggcg atcgacacga cgccgacaac
     1861 gaggcaaggg gagacatgtc gacacaacgt tcaacgattg gccgcgatgg agaaaccgtc
     1921 gcggccaatt ggtatctgag ggcgggatac gaaatcgagg ctcggaactg gcactgccgc
     1981 gagggcgaac tcgacatcgt cgcccatcgc atcgacgaac gcggagtcgc ctgcgtcgtg
     2041 gtgtgcgagg tcaagacccg atcctcggct cgattcggat cggcggccga ggcggtgacc
     2101 cggtcgaagc agtacaagat ccgtgccgcg acggtgcggt acctcgctca acgcgatggc
     2161 gggccgtgtt cgatccgctt cgacgttgcg gtcgtcgatc gatcaccgca cgggtatgga
     2221 gtgcgtgtga tcgagggtgc gttctgagcg gggcccgggt cagcgccaac ccttccagcg
     2281 cggcaaccct gccggcgcgg cgtcagattc cggtgccgag caaggcccag gccaccgcgg
     2341 cgacggcgat cgccacggcg gcgacgatga gccagacatc gatcgggcgg cgtttgtact
     2401 tgtggtgcgg gttgaacccc atggtgaacc tcggtgccct cgcagcgata ggacccccag
     2461 tattgtgcgg acatgagcga cctttcgacc cccgacgatc cggcagcgga gaacggcgcc
     2521 gggggcggtg atgagaccgc cgggatgctg accgaggagc aggctcgtgt gctcggcagc
     2581 ctgatcgaga aatcgcgcac cacccccgac gactacccgc tgacggcgaa cgcgttgatg
     2641 cgtgcgtgca accagagcac ctcgcgcgat cccgtggtga actacgacca gcgccaggtc
     2701 gaacgggtcg tcgcggagtt gaaggcgatg gggctcgtcc ggttcgtcca catggcgacc
     2761 ggtcgtgcgg tgaccaagta ccgacacgtg gtcgatgagg cgtggggact cgacccgcag
     2821 g
//
LOCUS       DAOOMA010000354         2820 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_43613_length_2820_cov_3.443761, whole genome shotgun sequence.
ACCESSION   DAOOMA010000354 DAOOMA010000000
VERSION     DAOOMA010000354.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 2820)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2820)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2820
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43613_length_2820_cov_3.443761"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..210
                     /locus_tag="PLP95_09510"
     CDS             <1..210
                     /locus_tag="PLP95_09510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015798683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl diphosphate synthase family
                     protein"
                     /protein_id="HPB46077.1"
                     /translation="YDPEMPEPELVVRTSGEIRTSNYLMWESAYSEWVFTDVLWPDFR
                     REDLYDSIAEYQQRERRFGGLSTDD"
     gene            213..932
                     /gene="recO"
                     /locus_tag="PLP95_09515"
     CDS             213..932
                     /gene="recO"
                     /locus_tag="PLP95_09515"
                     /inference="COORDINATES: protein motif:HMM:TIGR00613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HPB46078.1"
                     /translation="MALYRDVGVVLRTYKLGEADRIVVFCTQGRGKVRAVAKGVRKTR
                     SRFGGRLEPGCHVVAQFYEGRGDLDIVSQVDLVESPAPLREDLDRMGRVAAMLEAVDQ
                     MSQEGEQSSALYDLLVGGLRAVANWDTPLVTPSFYCKLLANDGVGLVVDGCVECGSPD
                     DLVSLDLNAGGLRCREHREGVSVSAQAVLILQLILGGRLAQALKLPIGAATFEVDHLA
                     SMAMERHLDRRLRSVHLLDER"
     gene            complement(1141..2079)
                     /locus_tag="PLP95_09520"
     CDS             complement(1141..2079)
                     /locus_tag="PLP95_09520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB46079.1"
                     /translation="MSDPNNPNSEGWIPPPPGMNPPPQSPPPQSPPPGGGYVPPPPPG
                     GGFTPPPPPQSPYSTPAGGFAPPPPTPGGGGVGTIDIGTVLSWAAAKYQQHAPVLLGL
                     TAVVAAATLIGNFISVKLIENAAGGVTISDDGSFRIQNSNGFWGGMFGGMILSIVISI
                     LVAILMIGITRAALKVTRGQAPSFADLTSGEHLAEYIVTSIVLWLLTAVGLVLCIIPG
                     LFVMFFMMMAPIHALDTGATVGDSISRSIAIVKANIVPIIVLVILAIIVSIMSSLIGG
                     MAGAIVAALIQLLLTPFTALASAAAYRQGNGEALAA"
     gene            2238..>2820
                     /locus_tag="PLP95_09525"
     CDS             2238..>2820
                     /locus_tag="PLP95_09525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012227726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase"
                     /protein_id="HPB46080.1"
                     /translation="MSDYLGQLDSAPEPDPTVFDKIVNLSKRRGIAFQSADIYGGFRS
                     TYDYGPIGVLLLRNVKDAWWRSMVQMRHDVVGLDAAILSPPAVWEASGHLANFTDPLV
                     DCTNCKSRHRLDKLDDPNVCPTCGAIGKFTEPRDFNLMFKTQAGPVAGAGADCFLRPE
                     TAQGMFINFINIVNSTRKKPPFGIAQVGKSFRNE"
BASE COUNT          484 a          856 c          980 g          500 t
ORIGIN      
        1 tacgaccccg agatgcccga accggaactc gtggtgcgca cctcggggga gattcgcacc
       61 tcgaattatc tgatgtggga atcggcctac agcgagtggg tgttcaccga cgtgttgtgg
      121 ccggactttc gccgggagga cctctatgac tccatcgccg agtaccagca gcgcgaacgt
      181 cgcttcggcg gcttgagtac cgacgactag ccatggcgct gtaccgcgac gtcggcgtcg
      241 tgttgcgtac ctacaaactc ggcgaggccg atcgaatcgt ggtgttctgc acgcaggggc
      301 gcggcaaggt gcgggctgtg gccaaagggg ttcgaaagac gaggtcgagg ttcggtggtc
      361 gcctcgaacc cgggtgccat gtcgtcgcgc agttctatga gggtcgcggc gatctcgaca
      421 tcgtgagcca ggtcgacctg gtcgagtccc cggcgccgct gcgagaggac ctcgatcgga
      481 tggggcgtgt ggcggcgatg ttggaggcgg tcgaccagat gagccaggag ggagagcaga
      541 gttcggcgct ctacgacctg ctcgtcggtg gcctgcgcgc cgtcgccaat tgggatactc
      601 cgctggtgac tccgtcgttc tactgcaagc tgctggccaa cgacggggtc ggcctcgtgg
      661 tcgacggctg tgtcgagtgc ggcagccccg acgatctggt cagcctggat ctgaacgccg
      721 gaggcttgcg ttgtcgcgag caccgcgagg gcgtcagcgt cagcgcgcag gcggtgctga
      781 ttctgcagct gatcctcgga ggccgcctgg cacaggcgct gaagctgccg atcggtgcgg
      841 cgaccttcga ggtcgaccac ctcgcgtcga tggcgatgga gcgacacctc gaccgtcggc
      901 tccgatcggt gcacctcctc gacgagcgct gattcgaaca gcgctgattc gacgagcact
      961 gactgcttgc gctgccgaac gcgggtttgc ccacagttca actgtgggca aacccggtcg
     1021 gcaggtgacc cgatgtgccc acagttgaac tgtgggcagc gatcgggggt ggtgcgggcg
     1081 gtgaggcctc gcgggcggtg aggcctcgcg ggcggtgagg cctcgcgggc ggtgccgggc
     1141 ctacgcggcg agggcctcgc cgttgccttg gcgatacgcg gccgctgagg cgagcgcggt
     1201 gaacggcgtc aacagcagct ggatcagcgc tgcgacgatg gcgccggcca ttccgccgat
     1261 gaggctgctc atgatgctga cgatgatcgc caggatcacc aacacgatga tcgggacgat
     1321 gttcgccttg acgatcgcga tgcttcgcga gatggagtcg cccacggttg caccggtgtc
     1381 gagtgcgtgg atgggggcca tcatcatgaa gaacatcacg aacaggcccg gaatgatgca
     1441 cagcaccagt ccgacggcgg tgagcagcca caacacgatg gaggtgacga tgtactccgc
     1501 cagatgctca ccggaggtga ggtcggcaaa cgacggggcc tgcccgcggg tgaccttgag
     1561 cgcagcccgc gtgattccga tcatcagaat cgcgaccagg atcgagatga cgatcgacag
     1621 gatcatccca ccgaacatgc cgccccagaa tccgttgctg ttttgaatcc tgaacgatcc
     1681 gtcgtcggaa atcgtcacgc caccggcggc gttttcgatg agcttcaccg agatgaagtt
     1741 gccgatgagg gtcgccgcag cgacgaccgc cgtcagaccc aggagcacgg gagcgtgctg
     1801 ctggtacttg gcggcggccc aactgagcac ggtgccgatg tcgatcgtgc ccacgccgcc
     1861 gccacccggg gttgggggtg gcggtgcgaa tccgcccgcc ggcgtggagt aggggctctg
     1921 cggcggtgga ggaggcgtga acccgccacc gggcggagga ggcggtacgt agccgccacc
     1981 gggtggcggc gactgcggag gcggggattg cggcggtggg ttcatcccgg gaggcggggg
     2041 aatccatccc tcggaattcg ggttgttggg atcgctcacg atggctcctg acgacgtggc
     2101 tttcgccgct caccgtaccg gagtcacgcc acaaaatcgc gtatctcgtc ggggtgttag
     2161 gcgatgtcga cgtcgagatg cgcgactcgg gatgtcgagg tgtggatcgc tgcagcgggc
     2221 cggtagcctc gcggaccatg tccgactatc ttggccaact cgattccgcg cccgaacccg
     2281 acccgaccgt ttttgacaag atcgtcaacc tgtcgaagcg tcggggcatc gcctttcagt
     2341 cggccgacat ctacggaggc ttccggtcga cttacgacta cggcccgatc ggcgtgctgt
     2401 tgctgcgcaa cgtcaaggac gcctggtggc gttcgatggt gcagatgcgc cacgacgtgg
     2461 tcggcctcga cgcagcgatc ctgagcccgc cggcggtctg ggaggcctcc gggcacctgg
     2521 caaatttcac cgatccgctc gtcgactgca ccaactgcaa gtcccggcat cgactcgaca
     2581 agctcgatga ccccaacgtg tgtccgacct gtggtgcgat cggcaagttc accgagcctc
     2641 gcgacttcaa cctgatgttc aagacccagg ccggcccggt tgcgggtgcc ggtgccgatt
     2701 gtttcctccg acccgagacc gcgcagggga tgttcatcaa cttcattaac atcgtgaatt
     2761 ccacccgcaa gaagccgccg ttcggcatcg cccaggtcgg aaagtcgttc cgcaacgaga
//
LOCUS       DAOOMA010000355         2817 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_43673_length_2817_cov_3.117306, whole genome shotgun sequence.
ACCESSION   DAOOMA010000355 DAOOMA010000000
VERSION     DAOOMA010000355.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 2817)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2817)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2817
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43673_length_2817_cov_3.117306"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..422)
                     /locus_tag="PLP95_09530"
     CDS             complement(<1..422)
                     /locus_tag="PLP95_09530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB46081.1"
                     /translation="MIKVVSADAVDAAALRGRLAAVRRLAGIAPAVCAPLDLDGAIIS
                     SLPGVDGHLDHVMLFAFADGRAPSPLLPADTEAIGATLALIHDAGRSIAHDDVPRFAV
                     IDVEAAALAPPSATDALLRVLERVRRFDGSAPAHAPPWR"
     gene            complement(2197..2355)
                     /locus_tag="PLP95_09535"
                     /pseudo
     CDS             complement(2197..2355)
                     /locus_tag="PLP95_09535"
                     /inference="COORDINATES: protein motif:HMM:NF013540.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
     gene            complement(2496..>2817)
                     /locus_tag="PLP95_09540"
     CDS             complement(2496..>2817)
                     /locus_tag="PLP95_09540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB46082.1"
                     /translation="DRFGPVAAGPVPVAAVVSTSYRASAVWSPEVGAGAEVVLALVDN
                     AVAARVSPERVLRHASAVARHGVAWFRGERGDAIAAANAILAAVDALAGQETPPAPPS
                     RGNR"
BASE COUNT          467 a          977 c          963 g          410 t
ORIGIN      
        1 cgccatggag gagcgtgggc aggcgccgac ccgtcgaagc gccggactcg ctcgaggacc
       61 cgtagcaggg cgtccgtcgc actcgggggt gcgagggcgg ctgcttcgac gtcgatgacg
      121 gcgaagcgcg ggacatcgtc atgggcgatc gagcgaccgg cgtcgtggat cagcgccagc
      181 gtggcaccga tcgcctcggt gtcggccggc aggagcggcg acggtgcacg cccgtcggcg
      241 aacgcgaaca gcatcacatg gtcgaggtgg ccgtcgaccc cggggagcga cgagatgatc
      301 gctccgtcga ggtcgagcgg cgcacacact gcgggcgcaa tgccggccaa gcgtcgtacg
      361 gcggcgagac gtccccgcag cgccgcggca tcgacggcgt cggccgacac gaccttgatc
      421 acacgtcggc gccgtcgtga cgggcgcgcc acacggtgct ctgatgcccc ccggcgagcg
      481 cctcgacatc ggtgattccc ggcagcgtgg ctactgccga tcccatgtcg ctcacgttcc
      541 gacgctagtg gttcgacggc cccgcggagg tcgacacgac cgtggcgaac aatttcgtcc
      601 atcgagcagg cggcgcgttc ggcgtgcgcc cggaaggaac agctcatgcg caaagcagcg
      661 ttgacgacag cgatcgtgat ggtcgtggtg accggagtcg cgtgctcgaa cggatcggtc
      721 gacggcccgg agagctccgc cgacgggacc cgaccggcac aggtcgagcc tgccgagcag
      781 cgcgacgatg cacgcgtcct ccgcgagctc gaggcagttc gtgcggcctt gcccgccgac
      841 ccatcgcctc ctccgacgac gggaaggtac ggctactcgg tctggcggtc ctacagcgag
      901 ccaggtggca tacgcgacga ggtcgacgcg atcgtgcgag cgcacccgga tcgggcacgg
      961 tcgatggtga tcggcacgtc gcatctcggc caggagatcg tggccgtacg agtgtcctcg
     1021 gacctgtcgt cggcgatcgg ggagcgtccc agcgtcgtcc acctcgccgg ccagcacgcg
     1081 cgggagtgga tcggcacgga gatcgatctg cgcctccttc gcctctacct ggacggcacc
     1141 gatcccgacg gggcacttgc tcgccaaccc gcagcaccga cctgtggttc gttccggtcg
     1201 cgaatcccga cggatacgac tacagcttca gcacggaacc gctctggcgg aagaacctgc
     1261 gggacaacga tggcgacggc cagatcaccg atgccgacgg cgtggacctc aaccgcaacc
     1321 tgccgtacaa gtggggcttc gacaacgagg ggtcgtctcc gctgcccggc gccccgacgt
     1381 accgtggccc gtctccggcg tcggtgccgc ctcctacacc acgtcatggg actcactcgg
     1441 ccacctcgcc accatgtcgt atccatcggg gaggaactcg cgatctgggc ttcaggcacc
     1501 gccacgagac cgcactccac ggccaggtcg gtgacgagcg actcgaacat cgctgaccca
     1561 ttgtgagggg tagcgacgaa atgcttgtgg tcggacccac gccaggtcgc cgcaggttcg
     1621 aactccagga ggaggcattc gttgcccatc tcactcccag cagcgacgac cgtggcctga
     1681 gaacgcgtgt cgtcgtgccc gtcggccgtg aggtcccacg gatcgctcac aaaaacgtcg
     1741 agatgcagcc ccagcagact cgacgcagcg atcaccaccg gcaggatagg agtcacggac
     1801 gactacgcag aagggcaggc gacggtgaac acgtccgaag gctgcggtcg gtcgtcggcc
     1861 ggtcggcaag tcagaatacc ggtcgtgcga gcgggagtag gcgtggtcac aatttggtca
     1921 cacacttccc gaaactcgcc aacagcggcc gacagcggcc gaactcgcca gtcctcgaaa
     1981 cgcccaattc gtgggcattc tgagggccgc cggcattggc tgacatccgc cgaacgcctc
     2041 gcccgtcgga gttcgagtcc tcccccccga gccaacgaag tagcaacggt gcccaggtca
     2101 acggcctggg cacctgtgcg agatggcacg agaagcctcc cgctggtcac gagttggtca
     2161 cgaacgtggc gggagcgagc gggacaagcc ggatggtcac gtgccgggct gggccaggtc
     2221 gctctcgatg gcggcgaaca gatcgctcat cgacatgccg aggccctggg ccaccctcgc
     2281 cacggtcgtg agcgacgtgt tgcgttgacc gcgctccagt ccgcccatgt aggcgcggtc
     2341 gatgccggcg agcgcggcga ggggcgctct tgcgtcgctt cacttccttg ttcaagcgtt
     2401 cgaggtcctc gtcggttcct gagggccggt accgcctgtt cggtcgatcg tgacggtctc
     2461 tcgcccctgc gggccgtgac ggggtcgggt gtgcttcacc ggttgccgcg gctgggcggc
     2521 gccggagggg tctcctgtcc ggcgagggcg tccaccgcag ccaggatcgc gttcgctgct
     2581 gcgatggcat caccgcgctc gccccggaac caggccacgc cgtggcgcgc caccgccgac
     2641 gcgtggcgca gcacccgctc gggtgacacc cgcgccgcca ccgcgttgtc gaccaacgcc
     2701 aacacgacct ccgctccggc cccgacctcg ggggaccaga ccgcactggc ccggtagctt
     2761 gtcgacacga ccgcagcgac cggcaccgga cccgcggcca ccggtccgaa ccggtcg
//
LOCUS       DAOOMA010000356         2814 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_43747_length_2814_cov_3.393983, whole genome shotgun sequence.
ACCESSION   DAOOMA010000356 DAOOMA010000000
VERSION     DAOOMA010000356.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 2814)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2814)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2814
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43747_length_2814_cov_3.393983"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..572
                     /locus_tag="PLP95_09545"
     CDS             <1..572
                     /locus_tag="PLP95_09545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB46083.1"
                     /translation="FPMVTIEGRRYLDGGVLNNVPINRAIELGATRIYVLHMGQHGKP
                     SHTVKRPVDAAMIAYWIARNGRFARDLATLPPHVEAFVLPPGERPDLKYDDFSQTAAL
                     IEQGYVNAVEFFAHQDRHREETTTVRAERLRRETRRVIDELRDKVPSLRSARGSQGYA
                     ELVDPDAAHDNDVAHDDGDPDRGDVASAP"
     gene            569..1003
                     /locus_tag="PLP95_09550"
     CDS             569..1003
                     /locus_tag="PLP95_09550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012228757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3151 family protein"
                     /protein_id="HPB46084.1"
                     /translation="MSEVRLIPSGIPETILPDEPAALTEAITAAQQRADDERRDALAS
                     VVARAPRSSAAWAALGSAGRDPIERYAAYRVGYHRGLDSLRANGWRGSGYVRWRHPSN
                     RGVLACFAGLMRSADEIGETDEVERLATFLRQLDPEWSDDNR"
     gene            1024..2265
                     /locus_tag="PLP95_09555"
     CDS             1024..2265
                     /locus_tag="PLP95_09555"
                     /inference="COORDINATES: protein motif:HMM:NF012503.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HPB46085.1"
                     /translation="MTESLESPPAFPQHGRSADDILSEVAELRPHDIDWRSGKAFSLV
                     YNYDDPELEHLQEQVALSFLHENALNPFRYRSLLQMETEVIGYAKALVGAQAGSLSSG
                     GTESIFLAVYTAREHARASGNTAPTLLCTDTAHPAFTKACHYLGVTEIRLAHGPDGRA
                     VPSAYREAIDDRTAIVVASTPCYPFGVIDPVTEIAAVALDAGVLCHVDACLGGWLLPW
                     WERLGRNVEPWDFRVPGVTSMSADIHKYGYTFKGASVVLYADPDLYQRQIFMFDDWPG
                     GLYASASSAGTRPGPPIASAWATINHLGADGYLRLAARVADATDRMIAGISAIDGLEV
                     FSNPDMSVFEFGSTVHDMDGICDRMDDRGWNLDRQQGGLHLMLAPGHDKVVDAFCADL
                     AEAASDHAPARGSGHTYGGVV"
     gene            2265..>2814
                     /locus_tag="PLP95_09560"
     CDS             2265..>2814
                     /locus_tag="PLP95_09560"
                     /inference="COORDINATES: protein motif:HMM:NF024214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTP transferase domain-containing protein"
                     /protein_id="HPB46086.1"
                     /translation="MALDGWIGAVLVGGDPSSDGTERALVDVDGVPIGSIALDALHGA
                     GIERVMLVGATDAHTAVLDGEPVDDVWPGEGSAGAILTALHHGFLSGASTVVVLACDL
                     PAVSSAAVARLIDTARSTSGSTEPQVVMVEEDGTARFPNGVYAMRLLPTLEARFADGA
                     LSVSDLIAEVPVLWVDGQGAFRQ"
BASE COUNT          441 a          977 c          953 g          443 t
ORIGIN      
        1 tgtttcccat ggtgacgatc gagggtcggc gctacctcga cggcggcgtg ttgaacaacg
       61 tcccgatcaa ccgcgccatc gaactcgggg ccacgcgaat ctacgtgttg cacatgggcc
      121 agcacggcaa gccgagccac accgtcaaac ggcccgtcga cgccgcgatg atcgcctact
      181 ggatcgcccg caacggccgc ttcgcccgcg acctggccac gttgccgcca cacgtcgagg
      241 ccttcgtgct cccgcccgga gaacgcccgg acctgaagta cgacgacttc agccagaccg
      301 cggcgctcat cgagcagggc tacgtgaacg ccgtcgagtt cttcgcccac caggatcgcc
      361 accgcgagga gaccacgacg gtgcgcgccg aacggctccg gcgcgagacc cgccgggtca
      421 tcgacgaact ccgcgacaag gtgccgtcgc tccgctcggc acgcggctca caggggtacg
      481 ccgagctcgt cgacccggac gcggcccacg acaatgatgt cgctcacgac gacggggacc
      541 ccgaccgggg cgacgtagca tcggcgccat gagcgaggta cgactgatcc cgtcggggat
      601 ccccgaaacc atcctgcccg acgagcccgc agcgctgacc gaggcgatca cagccgcgca
      661 acagcgtgcc gatgacgagc gccgcgacgc cctcgcgtcg gtcgtcgccc gggccccacg
      721 gtcgagcgcg gcgtgggctg cgctgggatc ggcgggacgc gaccccatcg agcgctacgc
      781 cgcctatcgg gtgggctatc accgcggcct cgactcgctg cgggccaacg gctggcgcgg
      841 cagcggctat gtgcgctggc gtcatccgtc gaaccgaggc gtcctcgcgt gcttcgccgg
      901 gctgatgcgt tcggccgacg agatcggtga gaccgacgag gtcgagcggt tggcgacgtt
      961 tcttcgccag ctcgatcccg agtggagcga cgacaaccgc tgaagggctc ctacagtcgt
     1021 gtcatgaccg aatctctcga gtccccacca gcgtttccgc agcacggccg gtcggccgac
     1081 gacatcttgt cggaggtggc ggagcttcga ccccacgaca tcgactggcg aagcggcaag
     1141 gcgttcagcc tcgtctacaa ctacgacgac cccgaactcg aacatctcca ggagcaggtc
     1201 gcgttgtcgt tcctccacga gaacgccttg aacccgtttc gctaccggtc gctgctgcag
     1261 atggagaccg aggtcatcgg ctacgccaag gcactggtcg gcgcccaggc gggttcgttg
     1321 tcctcgggcg ggaccgagtc gatcttcctc gccgtctaca cggcccgtga acacgccagg
     1381 gcatcgggca acacggcgcc gacgctgttg tgcaccgaca ccgcccaccc ggcattcacc
     1441 aaggcgtgcc attacctcgg ggtcaccgag atccgactgg cacacgggcc cgacggacga
     1501 gcggtgccga gcgcatatcg cgaggccatc gacgaccgca cggcgatcgt ggtcgcgtcg
     1561 acgccgtgtt accccttcgg ggtcatcgac ccggtcaccg agattgcggc ggtcgccctc
     1621 gacgccgggg tgttgtgcca cgtcgacgcc tgcctgggcg gctggctcct gccgtggtgg
     1681 gaacggttgg gccggaacgt ggagccgtgg gacttccggg tgcccggggt gacctcgatg
     1741 tcggccgaca tccacaagta cggctacacc ttcaagggcg catcggtggt cctgtacgcc
     1801 gacccggatc tgtatcagcg gcagatcttc atgttcgacg actggcccgg cggcctctac
     1861 gccagcgcct cgtcggccgg cacccgcccg ggccccccga tcgcctcggc gtgggcgacg
     1921 atcaatcacc tcggagccga cggctacctt cgcctcgccg cccgggttgc cgacgccacc
     1981 gaccgcatga ttgccggaat ttccgcgatc gacgggctcg aggtgttctc gaaccccgac
     2041 atgtcggtgt tcgagttcgg atccaccgtg cacgacatgg acggaatctg cgaccggatg
     2101 gacgaccgcg gctggaacct cgaccgccag cagggcgggc ttcacctgat gctggccccg
     2161 ggtcacgaca aggtcgtcga cgcgttctgc gccgatctcg ctgaggcggc gagcgatcac
     2221 gcgccggcgc ggggttccgg gcacacctat ggcggggtcg tgtgatggcg ctcgacgggt
     2281 ggatcggtgc cgtactggtc ggcggtgacc cctcgagcga cggcaccgag cgcgcactcg
     2341 tcgacgtcga cggggttccg atcggctcga tcgcgctcga cgcgttgcac ggagccggga
     2401 tcgaacgcgt gatgctcgtc ggggccaccg acgcccacac cgcggtgctc gacggcgaac
     2461 cggtcgatga cgtgtggccg ggggaggggt cggccggagc gatcctgacg gcgcttcacc
     2521 acggattcct gtccggggcg tccaccgtcg tggtgctcgc atgtgatctc cccgcggtgt
     2581 cctcggcggc ggtggctcgg ctgatcgata ccgcccgatc cacctcggga tcgactgaac
     2641 cgcaggtcgt gatggtcgaa gaggatggca ctgcgcgctt tccgaacggg gtatatgcga
     2701 tgaggctcct gccgacgctc gaggcacgct ttgccgacgg cgcactttcg gtttcggacc
     2761 tcatcgcgga ggtcccggtg ctgtgggtcg acgggcaggg ggcgtttcga cagc
//
LOCUS       DAOOMA010000357         2808 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_43896_length_2808_cov_2.932074, whole genome shotgun sequence.
ACCESSION   DAOOMA010000357 DAOOMA010000000
VERSION     DAOOMA010000357.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 2808)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2808)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2808
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43896_length_2808_cov_2.932074"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..322)
                     /locus_tag="PLP95_09565"
     CDS             complement(<1..322)
                     /locus_tag="PLP95_09565"
                     /inference="COORDINATES: protein motif:HMM:NF013396.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase domain-containing protein"
                     /protein_id="HPB46087.1"
                     /translation="MSDPLHPIVIDRLVDLADAAGGRVEFSSGRRSTAESVAISRVMF
                     DSRSVEAGDLFVALVADRDGHEFASDAIERGASAALVSREVDLDAPRILVEDTVDALS
                     RLGAA"
     gene            466..1560
                     /locus_tag="PLP95_09570"
     CDS             466..1560
                     /locus_tag="PLP95_09570"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF002528.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase family protein"
                     /protein_id="HPB46088.1"
                     /translation="MAAPSSRTPVSLPDHVRIVLLYGGASAEHDVSRVSAANVFAAAD
                     KDRYDITLFGIDRDGRWHLVGSDGPRHTDEGLGGLAVEGEVVSPSTALSAIIDAASDP
                     VVVVPVVHGPNCEDGSLQGLLELLDVAYVGPGVLSSAVSMDKAMAKTVLDAAAIPQGR
                     WDSGFEWELDDARLDAIAEHLGFPIFVKPANMGSSVGVTRANDRAELDTSVREALRFD
                     ELLVFEEMITGREVEIAVLGNESPRVSVPGEIVAGAEFYDFDDKYRDGVSKTLVPAAL
                     DDAQVAEMQDLALRTYRALRAEGLSRVDMFFDADGRGFLVNEINTLPGFTSISMYPTL
                     WEASGLGYRELIDELIRLALDRHARRRRSN"
     gene            complement(1729..>2808)
                     /locus_tag="PLP95_09575"
     CDS             complement(1729..>2808)
                     /locus_tag="PLP95_09575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB46089.1"
                     /translation="VARLGLVSVAALLGVSWWNERPQTFGYLALAAALLVVVERRSTW
                     WLAGIFAVWVPLHGSWAMGVPFVGALIFVRVMESRAFGRREIGEVAAMVAGSCLGALA
                     TPYGWSLLTFPLEQLGRESLILIVEWRPLRLDSWMSMVFLAQGIAGSAALLWRRRWVR
                     SAVALGMLALSIDAVRYAPLCAIAMVPLVAPAFAGLGTEEGISSPRRSHVAIVSGALG
                     VAVVALMMWTPSYDLSHYPTSAVDELEARTWVGAERAELPHILTPDWVGNYLEWRYGT
                     EAKPWLDDRAEVHDTVELARLVALLGEDPSRYERVIDAIDDDIVLWQSDTPFVRWLAE
                     HEDYRIVDEVGEWTIACRVASGFCP"
BASE COUNT          491 a         1000 c          899 g          418 t
ORIGIN      
        1 cagcagcacc gagacggctg agcgcgtcga cggtgtcctc gacgaggatc ctcggcgcgt
       61 cgaggtcgac ctcgcgagac acgagtgccg cgctcgctcc ccgctcgatg gcgtcgctcg
      121 cgaactcgtg accgtcgcgg tcggcgacca aggcgacgaa caggtcgccg gcctcgacgc
      181 tgcgcgaatc gaacatgacg cgcgagatcg ccaccgactc ggcggtcgaa cggcgccccg
      241 acgaaaactc gacacggccg ccggcggcat cggcgaggtc gacgagacgg tcgatgacga
      301 tggggtgcaa cggatcgctc accactccat cctgccagcc gccggcttcc gcccgctgcc
      361 gctgggttgc cgctgggttc cgggtgaatg cagccgggtt cccggggcag gcctcccggg
      421 gcaggcctcc cggggccacg gtcagtgccc gcgtaagctc cgggcatggc cgcccccagt
      481 tcccgcacgc cggtttcgct tcccgatcac gtccggatcg tgctgctcta cggcggagca
      541 tcggccgaac acgacgtctc gagggtgtcg gccgccaacg tgttcgccgc cgcagacaag
      601 gaccggtacg acatcaccct gttcgggatc gatcgcgacg gccgctggca cctcgtcggc
      661 tccgatgggc ctcgccacac cgacgagggc ctcggcggcc tcgccgtcga gggcgaagtc
      721 gtgtcgcctt cgacagccct gagcgcaatc atcgacgcgg cgtccgatcc ggtcgtcgtg
      781 gtaccggtcg tgcacgggcc gaactgcgag gacggttccc ttcagggcct gctcgaactc
      841 ctcgatgtcg cctatgtcgg cccgggcgtg ttgagctccg cggtatcgat ggacaaggcg
      901 atggccaaga cggtgctcga cgccgccgcg atcccccaag gccgttggga ctccggcttc
      961 gaatgggaac tcgacgacgc acgcctcgac gccatcgccg agcacctggg ctttccgatc
     1021 ttcgtgaagc cggcgaatat gggcagttcg gtcggggtca ccagagcgaa cgaccgcgcc
     1081 gaactcgaca cgtcggttcg cgaggcactg cggttcgacg aactgctggt cttcgaggag
     1141 atgatcaccg gccgcgaggt cgaaatcgcg gtgctgggca acgagtcgcc tcgggtatcg
     1201 gttcccggcg agatcgtcgc cggcgccgag ttctacgact tcgatgacaa gtaccgcgac
     1261 ggtgtgtcga aaaccctggt gcccgcggcg ctcgacgacg cacaggtcgc cgagatgcag
     1321 gacctggcac tgcgcaccta tcgggccctg cgggccgagg ggttgtctcg ggtcgatatg
     1381 tttttcgacg ccgacggtcg aggctttctc gtcaacgaga tcaacacgtt gcccggattc
     1441 acgtcgatct cgatgtaccc gacgctctgg gaggcctctg gacttggata ccgcgagttg
     1501 atcgatgaac tcatccggct agcgctcgac cgccacgccc gacgccgccg ctccaactga
     1561 tctcccgcgc cagcctgttc ctgcccgcgc cagcctgttc ctgcccgcgc cagcctgttc
     1621 tccgataacc agtggtcgtg atcacgacca ctggttatcg gagaacgggc caggggcggg
     1681 gtcagggcgg gggcatggca gggtcggcgg gggcagggca gggtcgggtc aggggcagaa
     1741 cccggaggcg actcgacagg cgatcgtcca ctcccccacc tcgtcgacga tccggtagtc
     1801 ctcgtgttcg gcgagccagc ggacaaacgg cgtgtcgctc tgccacaaca cgatgtcgtc
     1861 gtcgatcgcg tcgatcaccc gctcgtatcg cgacgggtcc tcgccgagca gcgccaccag
     1921 acgcgccaac tccacggtgt cgtgcacctc ggcgcggtcg tccagccacg gcttcgcctc
     1981 ggtgccgtaa cgccactcga ggtaattgcc cacccagtcc ggcgtcagga tgtggggcag
     2041 ttcagcgcgc tcggcaccga cccatgtccg ggcttcgagc tcgtcgaccg ccgaggtcgg
     2101 atagtgcgac aggtcatagc tgggtgtcca catcatcaag gccacgaccg ccacgccgag
     2161 cgctccggac acgatcgcaa cgtgtgatcg ccgcggcgac gagattccct cctcggttcc
     2221 aagaccagcg aaggccggtg cgacgagggg caccatggcg atcgcgcaca acggcgcgta
     2281 gcgaaccgca tcgatcgaca acgccaacat ccccagcgcc acggctgaac gcacccacct
     2341 ccgacgccac aacaacgccg cactcccggc aatgccctgg gccaggaaca ccatcgacat
     2401 ccacgaatcc aaccgcaacg ggcgccactc gacgatcaag atgagcgact cgcggccgag
     2461 ctgctcaagc gggaaggtca acaacgacca tccgtacggg gtcgccagag ctccgaggca
     2521 cgagccggca accatcgctg ccacctcacc gatctcgcgt cggccgaacg cgcgcgattc
     2581 cattactcgc acgaagatca gcgccccgac gaatggcacc cccatcgccc acgaaccgtg
     2641 cagcggcacc catacggcga agatcccggc cagccaccat gtgctgcgcc gctccaccac
     2701 gaccaacagt gcggcagcga gtgccaggta cccgaacgtc tgtggccgct cgttccacca
     2761 gctcaccccc aagagcgccg cgaccgagac gaggccgagg cgggcgac
//
LOCUS       DAOOMA010000358         2807 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_43912_length_2807_cov_3.662791, whole genome shotgun sequence.
ACCESSION   DAOOMA010000358 DAOOMA010000000
VERSION     DAOOMA010000358.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 2807)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2807)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2807
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43912_length_2807_cov_3.662791"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..846
                     /locus_tag="PLP95_09580"
     CDS             <1..846
                     /locus_tag="PLP95_09580"
                     /inference="COORDINATES: protein motif:HMM:NF025393.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="questin oxidase family protein"
                     /protein_id="HPB46090.1"
                     /translation="MSLVALYQLGATSQRLQDEFDRHGDGSAEARSDVAELHERVDEV
                     HALGIAEVIRRRAPSLVDGPATALFHPLIRLAYGIEADHGGQVAAALLDWETRFHVLH
                     HGMLEAGSRRPTEVLRALGNSEVEWPTTWDLDAVARGAEFGAALDGLATENLTIDDVA
                     ELALMAHCSSESFVTLHLVTAARAARSVASLLDDGSQRRLCRAMVVESAVAYAATGAP
                     RVATVEELDQRRGAALPSREAIAAKAIASSDPHVIKLVNVALSEELRTGDLLYRDFAA
                     HAVAE"
     gene            complement(913..2403)
                     /locus_tag="PLP95_09585"
     CDS             complement(913..2403)
                     /locus_tag="PLP95_09585"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HPB46091.1"
                     /translation="MADTSAPPFAELGFTDLGVPESVAAAAADSGFDTPFPIQTATLP
                     DSIAGRDVLGRGRTGSGKTLAFALPVVIRLMANDREREPGRPRALILAPTRELASQIY
                     RTVDGIARPLGFRTATVYGGIPAKPQIAAFRKGVDIVVACPGRLEDLLQQKALTLQGI
                     EITVLDEADHMADLGFLPSVKRLLDRTPAGTQRLLFSATLDRGVDGLVKQYLRNPVTH
                     SVDSAESPVATMEHHLLNVTSENRMAVLIELLSTPGKTIVFTRTKHKAKALARSLNKN
                     GIPSVDMHSNLAQNARVRNLAAFSDGRATTMVATDIAARGIHVDDVALVIHADPPVEH
                     KAYLHRSGRTARAGNDGTVVTLVLPEQRGDVADLVRKAKISPTTTNATPDHPIIAELA
                     PGERRRFTREEIAELLPFTVPQPASTKPGAKSGAKSGGKGSGGKSSGRSAGKATGRKP
                     AARNGGGSAGSRHRDDRQPAGSGRPTGPGRPTRKKPTAAGRSSRPR"
     gene            2620..>2807
                     /locus_tag="PLP95_09590"
     CDS             2620..>2807
                     /locus_tag="PLP95_09590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB46092.1"
                     /translation="MSLSPKPAARTVEAVDELLAEMAGLRARMAEHPDLADELSQLEV
                     NRWIFSITQVALDCFVWA"
BASE COUNT          440 a          883 c          992 g          492 t
ORIGIN      
        1 atgagtctgg tggccctcta ccaactcggg gcaacctcgc agcgtctcca agacgagttc
       61 gaccgtcatg gcgacggcag tgcggaggct cgtagcgatg tcgccgaact ccacgaacgt
      121 gtcgacgagg tacatgcgct cggtatcgcc gaggtgatcc gacggcgtgc tccatcgctc
      181 gtcgacggac cggcgacggc gctgtttcat ccgctgatcc gtctcgccta cgggatcgag
      241 gcagatcatg gcgggcaggt cgcggccgcg cttctcgatt gggaaacacg gtttcacgtg
      301 ctgcatcacg gcatgctcga agcaggttca cgacgtccga ccgaggtgct ccgcgcactg
      361 gggaattcgg aggtcgagtg gccgacgacg tgggatctcg atgctgtagc gcgcggagcg
      421 gagttcggag ccgcactgga cggcctcgcg accgagaacc tcacgatcga cgacgtcgca
      481 gagctcgcgc tgatggcaca ctgctcgtcg gagtcgttcg tcaccctcca tctggtcacc
      541 gcggccaggg cggcacgtag cgtcgcgagt ctcctcgacg acgggtcgca gcggcgtctt
      601 tgccgcgcga tggtcgtcga gtcggccgtg gcctacgccg cgaccggcgc tccaagggtt
      661 gccaccgtcg aggaactcga ccagcggcgg ggtgccgcct tgccgtcacg cgaggccatt
      721 gccgctaagg cgatcgccag cagcgatcct cacgtgatca agcttgtcaa cgtcgctcta
      781 tcggaggagt tgcgcaccgg cgacttgctg tatcgcgact tcgcagccca cgccgtggcg
      841 gaatgaccgc gatgcatctg tcgtagtggc ggtgaccaca accactacga cagacactcc
      901 gggagggtga ggtcagcgtg ggcgggagga gcggccggcg gctgttggct tcttgcgcgt
      961 cggtcgtcct ggtccggtcg gccgcccaga acctgcgggt tggcggtcgt ccctgtggcg
     1021 ggagcctgcc gagccaccgc cgttcctcgc ggccggcttt cgaccagtgg ccttgccggc
     1081 ggacctcccc gaactcttcc cgcccgaacc cttcccaccc gatttcgcgc cggatttcgc
     1141 gccgggcttc gtggatgccg gctgaggaac ggtgaacggc aacaactccg cgatctcctc
     1201 gcgggtgaaa cgccgacgtt cgccgggcgc caactcggcg atgatcggat gatcgggtgt
     1261 cgcgttggtc gtcgtcggcg agatcttcgc cttgcggaca aggtcggcga catcgccgcg
     1321 ctgttcgggc aacaccaggg tgaccacggt gccgtcgttg ccggcccgcg ccgtgcggcc
     1381 cgaccggtgc aggtacgcct tgtgctcgac cggcgggtcc gcgtgaatca cgagagccac
     1441 atcgtcgacg tgaatgcctc gcgccgcgat gtcggtggcg accatcgtcg tcgcacggcc
     1501 atcggagaac gccgcgaggt ttcgtactcg ggcgttctgg gccaggttcg agtgcatgtc
     1561 gaccgacggg atgccgttct tgttgagcga ccgggcgagc gccttggcct tgtgcttcgt
     1621 gcgcgtgaac acgatcgtct tgccgggggt cgacagcaac tcgatcagca ccgccatgcg
     1681 gttctccgag gtcacgttca gcagatgatg ttccatcgtg gcgaccgggg actcggcact
     1741 atcgacactg tgagtgacgg gattgcggag gtactgcttg acgagtccgt cgactccgcg
     1801 atcgagcgtc gccgagaaca gcagtcgctg tgtgccagcg ggggttcggt cgagcagccg
     1861 cttcaccgac gggaggaatc cgaggtcggc catgtgatcg gcctcgtcga gcaccgtgat
     1921 ctcgatgccc tgaagggtca gcgccttttg ctgcagcagg tcttcgaggc ggccaggaca
     1981 ggcgacgacg atgtcgacac ccttgcggaa cgctgcgatc tgcggcttgg cgggaatgcc
     2041 gccgtacacc gtcgcggtgc gaaagccgag ggggcgggcg atgccgtcga ccgtgcggta
     2101 gatctggctc gccaactccc gggtgggggc caggatcagc gcgcgtggac ggccgggctc
     2161 acgctcgcgg tcgttcgcca tgaggcggat gacgacggga agcgcgaagg ccagcgtctt
     2221 gccggacccg gtgcggccac ggccgaggac gtcgcgaccg gcgatggaat cgggaagggt
     2281 tgcggtttgg atggggaagg gggtgtcgaa cccgctgtcg gccgctgcgg ccgcgacgga
     2341 ttcggggacg ccgagatcgg tgaaaccgag ttcggcgaac gggggcgcgg acgtgtccgc
     2401 catgatgttg ctcctcgctg aggggtccgc cgaagtcatc ggtcgaggtc gatcatgtgg
     2461 cgcaaccaat gtgcgccggt gacctgcgac ggcggttcag cggggtcgct cactcgaacg
     2521 acccggatac tctaccggac cgatagcgtg caccgcacac acggcaccgg cgacatcgcc
     2581 cgtttcgtgt ggaggggaac cacacctgaa gggggagcca tgtcgctgtc accgaagcct
     2641 gccgctcgta ccgtcgaggc ggtcgatgaa ctcctggcgg aaatggctgg gttgcgcgcc
     2701 aggatggccg aacatccgga cctggctgac gagttgtccc aactcgaggt caaccggtgg
     2761 atcttctcga tcacccaggt ggcgctcgac tgcttcgtct gggccga
//
LOCUS       DAOOMA010000359         2804 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_43987_length_2804_cov_3.777374, whole genome shotgun sequence.
ACCESSION   DAOOMA010000359 DAOOMA010000000
VERSION     DAOOMA010000359.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 2804)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2804)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2804
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43987_length_2804_cov_3.777374"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1245
                     /locus_tag="PLP95_09595"
     CDS             <1..1245
                     /locus_tag="PLP95_09595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB46093.1"
                     /translation="AAATTGASTRISDGTVVSGAAAVVIPLPHAAHALAAVVQRPETG
                     TVPTTLDRPSVPRVEQVVRGWSAHLGSSPELHSGVDRRDALVRSAAADVLLGPDWDNT
                     DAVEVALVAEALGRLGRVDLFDAMTALLNDLRPNGSFDAPDPFSASLQFIRAVSATWS
                     GGCAPDAGERLLEAAALAVHYLSKRRNRAAVAARRSEVSFALESLIGLADDIGEGELA
                     ERLRGRVGELGVGSPLVGQRADEWVHALGAGFSFGFDAASQPPSVAASAAFVIRATCE
                     LAVERADGLDVFSGYVPSLAGREIDVRQLPTRWGIVSYSLRWHGRRPAVLWEMTPWRG
                     RSAAHEFALTASTLDPRWQHRGSEGEALLAEPAEADFETPPQFVHDHLLSESSDGHLH
                     GDWDAVPDFGDTSDLPDAGTFT"
     gene            complement(1289..1912)
                     /locus_tag="PLP95_09600"
     CDS             complement(1289..1912)
                     /locus_tag="PLP95_09600"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025953.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HPB46094.1"
                     /translation="MTSADSTTAALHAGQTAHRELPRVDATLLYNLWRWSRFRAYRTL
                     LGRRFEHSGRGLLGRRSRLQVRPGGSLRIGDRFVTGDDVLIGATGRIQLGTNVFINSF
                     SRIVAHDSIVIGDNVVIASDVSVLDHDHLTHTLDGHLVVENAKFVSKPIRIGSNVWLG
                     DKVTVLKGVTIGDNVIVGANAVVTSDVEPGTIVAGVPARPIRSIDLG"
     gene            complement(1976..>2804)
                     /locus_tag="PLP95_09605"
     CDS             complement(1976..>2804)
                     /locus_tag="PLP95_09605"
                     /inference="COORDINATES: protein motif:HMM:NF014213.2"
                     /note="catalyzes the removal of amino acids from the N
                     termini of peptides; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="M18 family aminopeptidase"
                     /protein_id="HPB46095.1"
                     /translation="AIHLDRDVTTAGLVLNKQQHLNPVWGLGDPEPGEFVGWLAEQVD
                     AVPDDIVGWDLMCHDLTPSRRLGVDRELLAAARLDNLCSSFGALDAVMHAAQFGASTT
                     VVAALFDHEEIGSESTTGVGGPLLGALVERLWEESGLNAAERMRARSDSRILSADMAH
                     GTHPNYPERHEPNHPIALGSGPVVKVNVNQRYATDALSGGEFRAVCESAGVPCQTYAH
                     RADLACGSTIGPISASRLGFATVDVGMAQLSMHSSRELMAVDDVDHMVRSFRAWAVA"
BASE COUNT          450 a          933 c          970 g          451 t
ORIGIN      
        1 gccgcggcga ccacgggtgc gagcacacgg atttcggacg gcacggtggt gtcgggcgcc
       61 gcggccgtcg tgattcccct gccgcacgcc gcccacgcac tcgccgcggt cgtgcagcgg
      121 cccgaaaccg gcaccgtgcc gaccaccctc gatcgaccaa gcgttcccag ggtggaacag
      181 gtggtgcgcg gctggagcgc tcacctcgga tcgagtccag aactgcacag tggggtcgac
      241 cggcgcgacg cgttggtgcg cagtgctgcg gccgacgtgc tgctcggtcc ggactgggac
      301 aacaccgacg ccgtcgaggt cgccttggtg gccgaggccc tcgggcgcct cggccgcgtc
      361 gacctgttcg acgcgatgac cgcgctgctc aacgacctgc gcccgaacgg cagtttcgac
      421 gcacccgatc cgttctcggc gtcgttgcag ttcatccgtg ccgtgtcggc cacctggagc
      481 ggaggttgcg ccccggacgc gggggagcgg ttgctcgagg ccgcggcgct ggccgtgcac
      541 tacctgtcca agcgccgcaa ccgcgccgcc gtcgccgccc gacgcagcga ggtgtcgttc
      601 gccctcgagt cgctcatcgg tctggccgac gatatcggcg agggcgaatt ggcggagcgg
      661 ttgcgcggcc gggtcggcga actcggcgtg ggttcgcccc tggtcggtca gcgggccgac
      721 gagtgggtgc atgcgttggg ggccgggttc tccttcgggt tcgacgctgc gtcgcaaccg
      781 ccgagcgtgg ccgcgtcggc ggcgttcgtc atccgcgcca cgtgcgagtt ggcggtcgaa
      841 cgagcggacg ggctcgatgt gttcagcggc tacgtaccga gcctcgccgg ccgcgagatc
      901 gatgtgcgcc aactaccgac ccgttggggg atcgtgtcgt actcgctgcg ctggcatggg
      961 cgacggcccg ccgtgttgtg ggagatgacg ccgtggcgcg gtcgcagcgc ggcgcacgag
     1021 ttcgcgctca ccgcctcgac attggatccg aggtggcaac accgtggcag cgaaggtgaa
     1081 gcgctgctgg ccgaaccggc ggaggccgac ttcgagacgc cgccgcagtt cgtgcacgac
     1141 catctgctga gcgagtcctc cgacggtcac ctgcacggag attgggacgc cgtgcccgac
     1201 ttcggcgata cctccgacct gcccgacgca ggcaccttca cctgacgccg gtcgcttcga
     1261 accctctcga tgcggtagac gctcgacttc agccgagatc gatggaacgg atcgggcggg
     1321 ccggaacacc ggccacgatg gttcccggtt cgacatcgga ggtgaccacg gcgttcgccc
     1381 cgacgatgac gttgtctccg atcgtgaccc ctttgagcac ggtcaccttg tcgccgagcc
     1441 acacgttgga cccgatgcga atgggtttcg acacgaactt ggcgttctcg accaccaggt
     1501 gaccgtcgag tgtgtgcgtg aggtgatcgt gatcgagcac cgacacgtcg gaggcgatca
     1561 ccacgttgtc gccgatcacg atcgaatcgt gcgcgacgat gcggctgaag ctgttgatga
     1621 acacgttggt cccgagctgg atacgacccg tcgccccgat gaggacgtcg tcgcccgtga
     1681 cgaagcggtc gccgatccgc agcgaaccgc cgggtctgac ctgcagccgc gaccgtcgcc
     1741 cgagcaggcc gcgcccggag tgttcgaagc gacggcccaa taacgttcgg taggcgcgaa
     1801 accgtgacca tcgccagagg ttgtacagca acgtcgcgtc gacacgagga agctcgcgat
     1861 gcgctgtttg gcctgcgtgc agcgcggcgg tggtggaatc agcggaggtc acgcctgcac
     1921 ggtaacgcga tcacgcccag atcacgccca gatcacgctc agatcccctt ccggattacg
     1981 ccacggccca ggcccgaaac gagcggacca tgtggtcgac gtcgtccacc gccatgagtt
     2041 cacggctcga atgcatggac agctgagcca tgccgacatc gacggtcgca aagccgagtc
     2101 gcgacgccga aatcggcccg atggtcgacc cacacgcgag atccgcccgg tgggcatagg
     2161 tctggcaggg aaccccggcc gactcacaga cggcacggaa ctcgccgccg ctgagggcgt
     2221 cggtggcgta gcgctggttg acgttcacct tcactaccgg accactcccg agagcgatcg
     2281 gatggttcgg ttcgtgccgt tcgggatagt tcggatgggt gccatgggcc atgtcggcgg
     2341 acaggatgcg cgaatccgaa cgcgcccgca tccgctccgc ggcgttcagc ccggattctt
     2401 cccacagccg ttctacgagc gcacccaaca agggcccgcc cactcctgtg gtggattctg
     2461 aaccgatttc ctcgtggtcg aacagggccg ccacaacggt cgtcgaggcg ccgaactgcg
     2521 cggcgtgcat caccgcgtcg agggccccga aggaggaaca cagattgtcg agtcgggcag
     2581 cggcgagaag ttcccgatcg accccgaggc gccgcgacgg cgtcaggtcg tgacacatga
     2641 ggtcccagcc cacgatgtcg tcgggaaccg cgtcgacctg ctcggcgagc cagccgacga
     2701 actcgccggg ttcggggtcg ccgaggcccc aaaccgggtt gaggtgctgt tgtttgttga
     2761 gtacgagtcc cgcggtcgtg acgtcgcggt cgaggtggat cgca
//
LOCUS       DAOOMA010000360         2798 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_44110_length_2798_cov_4.348888, whole genome shotgun sequence.
ACCESSION   DAOOMA010000360 DAOOMA010000000
VERSION     DAOOMA010000360.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 2798)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2798)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2798
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44110_length_2798_cov_4.348888"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            82..582
                     /locus_tag="PLP95_09610"
     CDS             82..582
                     /locus_tag="PLP95_09610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015798893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HPB46096.1"
                     /translation="MSDALKLEAIIEIPRGSRNKYEIDHETGALWLDRYLYTSMAYPT
                     DYGYLENTLGGDGDPLDALVLLNESVVPGTHLWVRPVAVYHMVDEAGDDAKVLCVPAK
                     DPRWDDIADLGDVNKFLLDEIAHFFESYKTLEPGKSAEKGEWADLASATKEIAESIER
                     FAAEGH"
     gene            complement(665..1414)
                     /locus_tag="PLP95_09615"
     CDS             complement(665..1414)
                     /locus_tag="PLP95_09615"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HPB46097.1"
                     /translation="MRFEGRTAIITGAASGLGRAVARRLASEGARVFGVDMNEAGLIE
                     TQGLITEAGGSMSFGTGDISDRMTAHGLVSVAMDDLGQIDTLVNCAGVMRAGHMTDVD
                     ESTFDLIYNVNVKGTFWMIQAAFPHLLDRNGSVVNIASNSALMGAPYNTVYSSSKAAV
                     VALTRSIAAEYIKTGVRVNAVAPGGINTPMMVADTFPPDTDWKLMQTMIGYRGASEPE
                     EVAAVVCFLASDEARSVHGSIYSADLGLTAF"
     gene            1483..2295
                     /locus_tag="PLP95_09620"
     CDS             1483..2295
                     /locus_tag="PLP95_09620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB46098.1"
                     /translation="MQDYPVKLGMMLFTLVDPHRGSEVAYNRWYERDHFYGGCMIGPG
                     ILAGSRWVATRDYKDLRFTGAGEFESPVAAPIDAGSYLATYFVQKGHEAEHFGWATPE
                     VFKLYEAGRGFDEREHAHTVLYSHTTAAYRDSDGVPVELALDHRFAGLTSVHLDRAEG
                     VSHRDLETWFEDSGRIGLLDETSPVAIAASWRPVIPKEGEGAAPMKLGSGPGTTQRSM
                     QLFFSDEAPAGHWDRFHAYAEAVEASGIARVVLAAPFLPTIPGTDTYTDQLW"
     gene            complement(2283..2660)
                     /locus_tag="PLP95_09625"
     CDS             complement(2283..2660)
                     /locus_tag="PLP95_09625"
                     /inference="COORDINATES: protein motif:HMM:NF013866.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HPB46099.1"
                     /translation="MVGFSMGASLALWAAARQPESFDRVVIYYGTSSLDFSAMTARVL
                     GHFVDDDVMVSDDDVTLLSAELFEGVRNPSCGTTPTPCTGLPSRVSTAAMTRRPRSSR
                     GNEPWTSSRTERDAASPPIGYQS"
BASE COUNT          506 a          950 c          874 g          468 t
ORIGIN      
        1 cgcgctgttc gacatgatcc cgtcgctgct caacggctga gataccgccg cccgagggcg
       61 accggtacga tgacccccgt tatgagcgat gcactgaagc tggaagcaat catcgagatc
      121 ccccgcggtt cccgcaataa gtacgagatc gatcatgaga ccggggccct gtggctcgat
      181 cggtacctct acacctcgat ggcgtacccg accgactacg ggtacctcga aaacaccctc
      241 ggcggcgacg gcgacccgct cgatgcactc gtgttgttga acgagtcggt cgtgcccgga
      301 acccacctgt gggttcgtcc ggtggcggtg taccacatgg tcgacgaggc gggggacgac
      361 gcgaaggtgc tctgtgtacc ggccaaggat ccccgttggg acgacatcgc cgacctcggc
      421 gacgtcaaca agttcctcct cgacgagatc gcgcacttct tcgagtcgta caagacgctc
      481 gagccgggca agtccgccga gaagggcgag tgggccgacc tcgcctcggc caccaaggag
      541 atcgccgaat cgatcgagcg tttcgctgcc gaaggccact gagtcgcagt agcgcagcgc
      601 accaacgcga gtgggccgga ccgtcgacgg tccggcccac tcgcgttctg gtcgcgactc
      661 gcgatcagaa cgctgtcaaa ccgaggtcgg cgctgtagat cgatccgtgc accgaccgcg
      721 cttcatccga cgccaagaag cacaccaccg ctgcgacctc ctcgggttcg gaggcaccgc
      781 ggtagccgat catcgtctgc atgagcttcc agtccgtgtc cggcgggaag gtgtcggcga
      841 ccatcatcgg cgtgttgatg ccgccggggg cgacggcgtt cacccgcacg ccggtcttga
      901 tgtactccgc agcgatcgac ctggtcagcg cgaccaccgc cgccttcgaa gatgaataca
      961 ccgtgttgta cggggctccc atcagcgccg agttcgaggc gatgttgacg acgctgccgt
     1021 tgcggtcgag cagatgcggg aacgccgcct gaatcatcca gaaggtcccc ttgacgttga
     1081 cgttgtagat cagatcgaag gtcgactcgt cgacgtcggt catatgaccc gcccgcatga
     1141 caccggcgca gttcaccagc gtgtcgatct gcccgagatc atccatcgct accgagacca
     1201 gaccgtgtgc ggtcatccgg tcgctgatgt cacccgttcc gaacgacatc gagccgccag
     1261 cctcggtgat caacccctga gtctcaatga ggccggcctc gttcatgtcg acgccgaaaa
     1321 cccgcgcccc ctcggaggca agacgacgtg ccacggctcg tccgagcccc gaagctgcgc
     1381 cggtgatgat ggcggtacgt ccttcgaatc gcatggaaac tcctccctga gcagcgctcc
     1441 acggcagccg ctttctgacg gttcgtcagg tacggtagcg ctatgcagga ctatcccgta
     1501 aagctcggca tgatgttgtt caccctcgtc gacccccacc gaggcagcga ggtggcgtac
     1561 aaccgctggt acgaacgcga tcacttctac ggcgggtgca tgatcggacc cggcattctc
     1621 gccggatcgc gttgggtggc gacccgcgac tacaaggacc ttcgcttcac cggcgccggc
     1681 gagttcgagt cgcccgtcgc cgcaccgatc gatgccggca gttacctcgc cacctacttc
     1741 gtccagaagg gccacgaggc cgagcacttc ggatgggcca cgcccgaggt cttcaagctc
     1801 tacgaggcgg ggcgcggttt cgacgagcgc gaacacgccc acaccgtgct ctactcccac
     1861 accaccgccg cctaccgcga ctccgatggc gtgcccgtcg agctcgcgct cgatcaccga
     1921 ttcgccgggt tgacctcggt gcacctcgac cgggccgagg gcgtctcgca ccgcgatctc
     1981 gagacctggt tcgaagacag cggccgcatc gggcttctcg acgagacctc accggttgcc
     2041 atcgcggcgt cgtggcgacc cgttatcccc aaggaaggcg agggagcggc tccgatgaag
     2101 ttggggtctg ggccgggcac cacccaacgg tcgatgcagc tgttcttttc cgacgaggcg
     2161 cctgcgggcc actgggatcg gttccacgcc tatgcggagg cggtcgaggc ctcgggaatc
     2221 gcccgggtcg tgttggcggc cccgtttctc ccgacgattc ccggcaccga cacctacacc
     2281 gatcagctct ggtagccgat cgggggcgag gcagcgtccc gttcagttct tgaggaagtc
     2341 cagggttcgt tgccacgcga gctccgcggc cgcctcgtca tagcagccgt gctcacccgg
     2401 ctcggcaaac cagtgcacgg cgtcggggta gtgccacagg acgggttccg aaccccctcg
     2461 aacagctcgg ccgacagcag ggtcacgtcg tcgtcggaga ccatgacgtc atcgtcgacg
     2521 aaatgcccga gcacccgcgc ggtcatcgcc gagaagtcga gcgacgatgt gccgtagtag
     2581 atgaccaccc ggtcgaagga ctccggctga cgggcggccg cccacaacgc gagcgaggcc
     2641 cccatcgaga atccgaccac cgagatcggc ccgctcaatc aggtgatgca tctgtggagc
     2701 tatgccgacc tcaacgagcg cgcgcggctg cgcgcggagc gttcgaagaa cccgcactgg
     2761 agaaaggaat acacgccgct gatccgcccg ctgctggt
//
LOCUS       DAOOMA010000361         2797 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_44144_length_2797_cov_3.070387, whole genome shotgun sequence.
ACCESSION   DAOOMA010000361 DAOOMA010000000
VERSION     DAOOMA010000361.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 2797)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2797)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2797
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44144_length_2797_cov_3.070387"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..259)
                     /locus_tag="PLP95_09630"
     CDS             complement(<1..259)
                     /locus_tag="PLP95_09630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB46100.1"
                     /translation="MNDSTEHSMVRVPVRFEDIAVGDTLTPVSIEISYKRICMNAAST
                     WDWFPGHHDPDYARSQGQRTIYLSTLFFHGFIDRGLNEWAGP"
     gene            complement(256..783)
                     /locus_tag="PLP95_09635"
     CDS             complement(256..783)
                     /locus_tag="PLP95_09635"
                     /inference="COORDINATES: protein motif:HMM:NF024844.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase N-terminal
                     domain-containing protein"
                     /protein_id="HPB46101.1"
                     /translation="MSAPTSDVTGLGMTFGTFDEGRAWVGHRSEPRHAWFPIDRSMVL
                     YYCSLVEDANPRYWEGEDCPPGLLMSLGFAPQWVPGYLARADMMFALSVPLPGHHIIN
                     ASTTTEFERRPRVGDHVSIVEEVASISEPKTTRVGTGVFITTVSTFSDQHGEIIGRNT
                     NVLFRYDTADSEGAS"
     gene            complement(780..1184)
                     /locus_tag="PLP95_09640"
     CDS             complement(780..1184)
                     /locus_tag="PLP95_09640"
                     /inference="COORDINATES: protein motif:HMM:NF013722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC/PaaZ C-terminal domain-containing protein"
                     /protein_id="HPB46102.1"
                     /translation="MALATGDEMPTSQFGPLTRSHIVRYAGSGGDFNPIHHDEEFARA
                     AGMPGVFGMGLLHGGVLAQRLTAWVGLANIRSFRIRFTGQVWPGDLLSFGGRVTSVRE
                     AGGQQVADLELVVTRQTGEPAIKGSAVVQVTR"
     gene            complement(1202..1660)
                     /locus_tag="PLP95_09645"
     CDS             complement(1202..1660)
                     /locus_tag="PLP95_09645"
                     /inference="COORDINATES: protein motif:HMM:NF024844.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase N-terminal
                     domain-containing protein"
                     /protein_id="HPB46103.1"
                     /translation="MADASLIGTQLGRTTFPVDRSKVREFALSLGDRDPIYQDVAAAR
                     AAGFGAIPAPPTFVVSSAHWRADDDMFGALGLDLRRVLHGECGWEYFAPVFVGDELTE
                     TRRVSNVTSREGKRGGIMTIVTIETDFTNQRDELVVRQTDVLIETGGSTQ"
     gene            complement(1653..2675)
                     /locus_tag="PLP95_09650"
     CDS             complement(1653..2675)
                     /locus_tag="PLP95_09650"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HPB46104.1"
                     /translation="MLGADLATLVETAEATDLAGFDAAWASEFYSRSGSISMAAMAAR
                     TKRCRIGSSILYGVGRSPLVLATEARDLDELSGGRVVLGLGNGTRRMMSDWHGVEDTS
                     APALRMEELVPLVRRIWNLHEGPVRHEGRFYRMNLVPTGDVAPPKRAIPIITAGVRPR
                     MCEVAGRVADGLAGHPLFTTAYVEEVARPAVVRGAERAGRDPADIEIVSMVICAVHDD
                     PQIARREAAQQIAFYSSVKTYEHVLDVSGFARQGAAIRDAFARRDLPAMFAAVTDDMI
                     DAMAVAGTAAEVREGLRRYEGVLDHIVLYSPSIGLAPERIAENLGSLIRDCAPAFAGG
                     KGDQSG"
BASE COUNT          452 a          938 c          969 g          438 t
ORIGIN      
        1 cgggtccggc ccattcgttg aggccgcggt cgatgaagcc atggaagaag agggttgaca
       61 ggtagatcgt gcgctggccc tggctgcgcg cgtagtcggg gtcgtggtga ccggggaacc
      121 agtcccatgt cgaagccgcg ttcatgcaga tgcgcttgta gctgatctcg atcgagacgg
      181 gcgtcagggt gtcgccgacg gcgatgtcct cgaaccgcac ggggacgcgg accatgctgt
      241 gctcggtgga gtcgttcatg atgcgccttc actatcggcg gtgtcgtatc ggaagagcac
      301 gttggtgttg cgcccgatga tctcgccgtg ctgatcggag aacgtactca ccgtggtgat
      361 gaacacgccg gtgcccaccc gcgtcgtctt cggttcggag atcgaggcca cctcctccac
      421 gatcgacacg tgatcgccca cccggggccg gcgttcgaac tccgtcgtcg tcgaggcgtt
      481 gatgatgtgg tggcccggca acgggacgct gagcgcgaac atcatgtcgg cacgcgctag
      541 gtacccgggc acccactgcg gcgcgaagcc gaggctcatc agcagcccgg gcgggcagtc
      601 ctcaccctcc cagtaacggg ggttcgcgtc ctcgacgagc gagcagtagt acaacaccat
      661 cgagcggtcg atcgggaacc acgcatgccg cggttcggag cgatgaccga cccacgcccg
      721 gccttcgtcg aaggtcccga acgtcatccc gaggccggtg acgtcgctcg ttggcgcgct
      781 caccgggtca cctgcacgac ggcgctcccc tttatcgcgg gttcgccggt ctggcgcgtg
      841 accacgagtt cgaggtcggc cacctgctgc cctccggcct cacgcacgct ggtgaccctg
      901 ccgccgaagc tgagcaggtc accgggccat acctgaccgg tgaaacggat gcggaacgag
      961 cggatgttgg ccagacccac ccaggcggtg agccgctggg cgagcacacc gccgtgcagc
     1021 agtcccatgc cgaacacacc gggcatgccg gccgcgcggg cgaactcttc gtcgtggtgg
     1081 atcgggttga agtcgccgcc ggagccggcg tagcggacga tgtgcgagcg cgtcagcggg
     1141 ccgaactgtg aggtcggcat ctcgtcgccg gtggccaacg ccaccggcgg gggggatgtc
     1201 gtcattgggt ggagcctccg gtttcgatca agacgtcggt ctggcgcacg acgagttcgt
     1261 cgcgttggtt ggtgaagtcg gtctcgatcg tcactattgt catgatgccg ccgcgcttcc
     1321 cctcgcggct ggtcacgttc gacacccgac gagtctcggt gagctcgtcg ccgacgaaca
     1381 ccggcgcgaa atactcccag ccgcactcac cgtgcagtac gcgtcgcagg tcgagcccga
     1441 gggcgccgaa catgtcgtcg tcggcgcgcc agtgagccga ggagacgacg aaggtcggag
     1501 gagcggggat cgcgccgaag ccggcggcgc gggcggccgc gacgtcctgg tagatcgggt
     1561 cgcggtcgcc gagcgaaagt gcgaactcgc gcactttgga ccggtcgacg ggaaacgtgg
     1621 tcctcccgag ttgcgtcccg atgagcgatg cgtcagccac tctggtcacc tttcccgccg
     1681 gcgaaggccg gcgcacaatc gcgaatgagg ctgccgaggt tctcggcgat gcgctcgggc
     1741 gcgaggccaa tcgagggtga gtagagaacg atgtggtcca gcacgccctc gtagcggcgc
     1801 agcccttctc ggacctcggc ggcggtgccg gccaccgcca tcgcgtcgat catgtcgtcg
     1861 gtgaccgcgg cgaacatggc cggcaggtca cgccgcgcga acgcgtcgcg gatggccgct
     1921 ccctgcctgg cgaaaccgct gacgtcgagc acgtgctcgt aggtcttcac cgaggagtag
     1981 aacgcgatct gctgcgcggc ctcacgacgt gcgatctgtg ggtcgtcgtg caccgcgcag
     2041 atgaccatcg agacgatctc aatgtcggcg ggatcgcgtc cggcccgctc cgcaccgcgc
     2101 acgacggctg ggcgggcgac ctcctcgacg taagccgtgg tgaacagcgg atgaccagcc
     2161 aacccgtcgg ccacgcgccc tgccacctcg cacatccgcg gacgcacgcc cgcggtgatg
     2221 atcgggatcg ctcgcttcgg cggcgcgacg tcgccggttg ggacgagatt catccggtag
     2281 aagcgcccct catggcgcac ggggccctcg tgcaggttcc agatccgacg cacgagcggg
     2341 accagctcct ccatgcgcag agcgggagcg gaggtgtcct ccacgccgtg ccagtcgctc
     2401 atcatgcggc gtgtgccgtt gccgagcccg agcacgacgc gcccgcctga aagctcgtcg
     2461 aggtcacgcg cctcggtggc gagcacgagc gggctgcgcc cgacgccata caggatcgac
     2521 gacccgatgc ggcagcgctt tgtgcgcgcg gccatcgcgg ccatcgagat cgagccggag
     2581 cgcgagtaga actccgaggc ccaggcggcg tcgaagccgg cgaggtcggt agcctcggcg
     2641 gtctcgacaa gggtggccaa gtccgcgccg agcaccgaga ccccgtacgt gctcacgacg
     2701 ccgccggcct gaatcggacc agcccaaggt cctcgttcac ccgctcgaac actgcgcgca
     2761 ctctcgcccc gatcgcaact tcatctgggg ccacgcc
//
LOCUS       DAOOMA010000362         2782 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_44476_length_2782_cov_2.596993, whole genome shotgun sequence.
ACCESSION   DAOOMA010000362 DAOOMA010000000
VERSION     DAOOMA010000362.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 2782)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2782)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2782
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44476_length_2782_cov_2.596993"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            9..917
                     /locus_tag="PLP95_09655"
     CDS             9..917
                     /locus_tag="PLP95_09655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HPB46105.1"
                     /translation="MAYFAENCTEYGLELPAPVPVERIEVGLSGGGVLSGLRWGTGPL
                     DDCAAVFLHGGAQNAHTWDTVLLALDQELSARSALCFDLPGHGHSSRRDDGWYDPTSN
                     AAAVAEAMDTLGVRDVVLVGMSLGGLSANVVAATRPDLVSNLVIVDVTPGVNPDKASA
                     IHAFIAGPQHFATFGEIFDRTVEFNPTRTPESLRRGILHNAHRVVEGKHAGSWEWNYD
                     RRKLDIDQMPPMDDLWDHVGAVAAPYLLVRGSTSPVVDDEDVAELLRRQPDARVVVVE
                     GAGHSVQGDDPLRLAELLVPLLPPRL"
     gene            1123..1329
                     /locus_tag="PLP95_09660"
     CDS             1123..1329
                     /locus_tag="PLP95_09660"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HPB46106.1"
                     /translation="MGLGSSLLRHRVALAASLGARSVTVDVLGWNERSRAMIERVGFE
                     YERSWPSRWESGHTVLGYRLWCTR"
     gene            complement(1389..2486)
                     /locus_tag="PLP95_09665"
     CDS             complement(1389..2486)
                     /locus_tag="PLP95_09665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HPB46107.1"
                     /translation="MDFSMSPALLELADEARTVAREFTADLDITEDSWLRGVDVEFPR
                     VMAEHGWLGMTWPTEWGGGGRSALERFVVVEALISEGAPLATAWFADRQIGPTLLQFG
                     TDAQRREFLPDLVAGTSKWCVGMSEPDAGSDVASLRTRADRDGDDWIVNGSKVWTSGA
                     AESDWCYLIARTDQNAKPYAGLSEFIVDLRSPGVEIRTIHDMSDDHHFCEVIFDDVRI
                     GGDRIVGTENAAFKQIMRQMEHERGGIDRLVSNRRLYLDARDRADISDPRVRNEIGRL
                     EGGYRIGRHLVLREVMGQAPQGFSAVTKTWCTEFEQRVAEFCVASFGAEAMLWNRASR
                     NICYAPGYTIMGGTTQILRNIIAERILGLPR"
BASE COUNT          429 a          918 c          957 g          478 t
ORIGIN      
        1 acgacgaaat ggcctacttc gccgagaact gcaccgagta cggcctcgaa ttgccggccc
       61 cggttcccgt cgagcgcatc gaggtcggcc tcagcggcgg tggggttctg agcgggcttc
      121 gctgggggac cggtccgctc gacgactgtg ccgcggtctt cctgcacggc ggggcacaga
      181 acgcacacac ctgggacacg gtgctgttgg cactcgacca ggagttgtcg gcacgctcgg
      241 cgctgtgttt cgacctgccc ggccacggcc actcctctcg tcgagacgac ggatggtacg
      301 acccgaccag caacgccgca gcggtcgccg aagcgatgga caccctcggg gtgcgcgacg
      361 tcgtgttggt ggggatgtcg ctcggcgggt tgagcgcgaa cgtcgtcgcc gcgacgcgtc
      421 ccgatctcgt atcgaacctc gtcatcgtcg acgtgacccc gggggtgaac cccgacaagg
      481 cgagcgcaat ccacgccttc atcgccgggc cacaacactt cgccaccttc ggcgagatct
      541 tcgaccgcac cgtcgagttc aaccccaccc gcacccccga gtcgctgcgc cgcggcatcc
      601 tgcacaacgc gcatcgggtc gtcgagggga aacacgccgg tagctgggag tggaactacg
      661 accgccgcaa gctcgacatc gaccagatgc ctccgatgga cgacctgtgg gaccacgtgg
      721 gcgcggtcgc cgctccgtat ctgctggtac gcgggtcgac ctcgccggtg gtcgacgacg
      781 aggacgtggc cgagctgttg cgccgtcagc ccgacgcccg ggtggtcgtc gtcgaggggg
      841 ccggacattc ggtgcagggg gacgatccgc tgcgcctcgc ggagctgttg gttccgctgc
      901 tcccgccacg gctctagctg ttcgctgaac gtttactcgt cgttggtggt ggaagcgtgc
      961 ggcgggctgt ttgcttcggg gtgtgctgac gctgtgtcca tcgccatcgc cgtcctcatc
     1021 accgtcctca tcgccatcgc tgggtgacct gtcgcaggcc ccggtcatcg cgtcgtcggt
     1081 cgtctggcgc atgcgtacgg caacttcgtc gaggacgccg ggatggggct tggttcgtcg
     1141 ttgctgcggc accgggtcgc actcgccgcc agcctcgggg cgaggagtgt caccgtcgac
     1201 gtcctggggt ggaacgaacg ctcccgagcg atgatcgaac gcgtcggatt cgaatatgag
     1261 cggtcctggc cctcgcggtg ggaatcgggc cacaccgtgc tcggttaccg gctctggtgc
     1321 acccggtgag cgggtggtga cgggctcggt cggcggccgc gatcgctgac cgcggtcggt
     1381 gaccgcggtc agcgggggag gccgaggatt cgttcggcga tgatgttgcg cagaatctgc
     1441 gtcgtgccgc ccatgatcgt gtagccgggt gcgtaacaga tgttgcggct ggcgcggttc
     1501 cacaacatgg cctcggcccc gaacgacgca acgcagaact ccgccacgcg ctgttcgaac
     1561 tcggtgcacc aggtcttggt caccgccgag aatccttgcg gcgcctggcc catgacctcg
     1621 cgcaacacga ggtggcgtcc gatgcgatag ccaccctcga gccgtccgat ctcgttgcga
     1681 acccgcgggt cggagatgtc ggcccggtcg cgggcgtcga ggtagaggcg acggttcgac
     1741 accagccgat cgatgccgcc gcgctcgtgt tccatctgac gcatgatctg cttgaacgcg
     1801 gcgttctcgg tgccgacgat gcggtcgccc ccgatgcgca cgtcgtcgaa gatgacctca
     1861 cagaagtggt ggtcgtcgct catgtcgtgg atcgtgcgga tctccacgcc gggggagcgc
     1921 agatcgacga tgaactccga cagcccggcg tacggcttcg cgttctggtc ggtgcgggcg
     1981 atcaggtagc accagtccga ctccgcggcc cccgaggtcc acaccttcga gccgttcacg
     2041 atccaatcgt cgccgtcgcg atcggctcgg gtccgaagcg aggcaacgtc ggaaccggca
     2101 tcgggctcgc tcatgcccac gcaccacttc gaggtgccgg cgacgaggtc ggggaggaat
     2161 tcgcgacgct gggcgtcggt gccgaactgc aacagcgtcg ggccgatctg gcgatcggcg
     2221 aaccaggcgg tggcgagggg agcgccttcg gagatgagcg cctcgacgac gacgaatcgt
     2281 tcgagcgcac tgcgcccacc gccaccccac tcggtcggcc aggtcatacc caaccagccg
     2341 tgttcggcca tgacccgggg gaactcgaca tcgacgccgc gaagccagga atcctcagtg
     2401 atgtcgagat cggcggtgaa ctcgcgggca accgtacggg cctcgtcggc cagttcgagc
     2461 agcgcgggcg acatcgagaa gtccatggcg gaaacctagc gccgccggcg ctgacgacga
     2521 gcttcccgcc ggccgtctca aactttgtgc cggaagcgac accagatgtc gcttccggca
     2581 aaaagtttgc cggatcgccg tcggttttga tggggcgacg tcgatcgagc cgtaatgtga
     2641 cccaccatgt ggcgccgcaa gatattggtc ggagtcggga tcgctgcggt cgtggcgctg
     2701 ttctcggggg cactcgtcct cgttcgacgc agcgagcgcc ccgccatccc cgacgcggtc
     2761 gacctgagtg tgccgctcgc gg
//
LOCUS       DAOOMA010000363         2772 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_44699_length_2772_cov_4.606919, whole genome shotgun sequence.
ACCESSION   DAOOMA010000363 DAOOMA010000000
VERSION     DAOOMA010000363.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 2772)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2772)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2772
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44699_length_2772_cov_4.606919"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(27..155)
                     /locus_tag="PLP95_09670"
     CDS             complement(27..155)
                     /locus_tag="PLP95_09670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB46108.1"
                     /translation="MMNVGLIARLGAGLRRRRLAALLGAGLLTTSAAACSPAAVPA"
     gene            complement(234..512)
                     /locus_tag="PLP95_09675"
     CDS             complement(234..512)
                     /locus_tag="PLP95_09675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB46109.1"
                     /translation="MDTSSEEAKSYYVDLSGMPGASRLIEHYAEVSGRQVDDMDYYTV
                     LARWKLAIVLEQSVKYGGDSPAAKALGPYVLNLMKSAADLAETSDYRG"
     gene            complement(515..844)
                     /locus_tag="PLP95_09680"
     CDS             complement(515..844)
                     /locus_tag="PLP95_09680"
                     /inference="COORDINATES: protein motif:HMM:NF013775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HPB46110.1"
                     /translation="MTASNASGGSSRHWTAPSWPEPFHSDPTKRPELAYQLVEGVALM
                     GAVDWEARGLGDLGRPDGYHDRQVERWLRFFDRVKTREVPGLAEATRWLQEHKPIDFV
                     PGIMHGD"
     gene            complement(988..2055)
                     /locus_tag="PLP95_09685"
     CDS             complement(988..2055)
                     /locus_tag="PLP95_09685"
                     /inference="COORDINATES: protein motif:HMM:NF014789.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HPB46111.1"
                     /translation="MRDRGLWACHLGPDLGGQGYGQVKLALMNEIIGRSSWAPIVFGT
                     QAPDTGNAEILAHYGTDEQKRRYLEPLLNGEVFSCYSMTEPGGGADPTLFTTRAVRDG
                     DEWVINGRKWFSSNAKTASFLIVMAVTDPDVSAYQGMSMFLVPAETPGVNIVRNTGLG
                     TEPLDEGTHAYIHYEDVRVPAGAVLGGEGQAFVIAQTRLGGGRIHHAMRTVGQCNKAL
                     EMVCERALSREVAGGLLADKQFVQGYIADTYAQLLQFRLMVLHVALGVSNEMPLASVL
                     LAGPVMGLADGPTEVHKVTVARQVLRDRRPAEGEWPSQHIPTRLAEVRATYAEILEHE
                     VGTHERADIRNARPRPARCMV"
BASE COUNT          476 a          981 c          826 g          489 t
ORIGIN      
        1 ccagccctgg cgcttcgtcg aggtcgtcac gccggcacgg cggcaggcga acacgctgcc
       61 gccgacgtgg tgagcaggcc ggcgcccagc agcgcggcga gcctgcgtcg gcgcaacccc
      121 gccccgagcc gcgcgatcag accaacgttc atcatctccc ccctcgtttc atcattcccc
      181 gcaaccgtaa gacatatcag cccgactgtc tcgcgatgac cgattgaact tgactacccc
      241 cgatagtccg aggtctcggc caggtcggca gcactcttca tgaggttcaa cacgtacgga
      301 cccaacgcct tggccgccgg actgtcaccg ccgtatttca cgctttgttc caacacgatg
      361 gcgagcttcc agcgggcgag caccgtgtaa tagtccatgt cgtcgacctg acgccccgac
      421 acctcggcgt aatgctcgat cagccgtgat gctccgggca tccccgacag atcgacgtag
      481 tagctcttcg cctcctccga ggaggtgtcc acttttagtc gccgtgcatg atgccgggca
      541 cgaagtcgat cggcttgtgc tcctgcagcc acctcgtcgc ctcagccaac ccggggacct
      601 cacgggtctt gacccgatcg aagaagcgca accagcgctc gacctgacgg tcgtggtatc
      661 cgtctggtcg accgagatcg cccaggccgc gcgcctccca gtcgacggcc cccatcagcg
      721 caacgccctc gacgagttgg tacgccagtt cgggtcgctt cgtcgggtcg ctgtggaacg
      781 gttcgggcca actcggtgcc gtccaatgcc tcgatgatcc gccactcgcg tttgatgccg
      841 tcatcacgag cgtcgggagc cttgggtgga ggcattcgca gcgccgcgtg caaaccgtcg
      901 cgccgcactt cgtagatgac gttctggatt ccgcccgaca cttcggtcag ttcgatcgga
      961 gctccggcag tgccgaggcc ccgctcgtca caccatgcac cgagccggtc gcggtcgagc
     1021 gtttcggatg tctgctcgct catgagttcc cacctcgtgt tccagaatct cggcgtacgt
     1081 agccctgacc tcagcgaggc gagtcgggat gtgttgggat ggccactcgc cctccgcagg
     1141 tcgacggtcg cgcagcacct gacgcgccac cgtcaccttg tggacctcgg tcggtccgtc
     1201 cgcgagtccc atcaccggac ccgcgagcaa caccgatgcc aagggcatct cgttcgacac
     1261 cccgagcgcc acgtgcagca ccatcagccg gaactgcaga agctgtgcgt aggtgtcggc
     1321 aatgtagccc tgcacgaact gcttgtcggc caacaaccct ccagcgacct cgcggctcag
     1381 ggcccgttcg cacaccatct ccagcgcctt gttgcattgg cccaccgtgc gcatcgcgtg
     1441 gtgaattcgt ccgcctccca ggcgggtctg ggcgatgacg aaagcctgcc cttcgccgcc
     1501 cagcacggca cctgcgggca cgcgcacgtc ctcgtagtgg atgtaggcgt gggtgccttc
     1561 gtcgaggggt tcagtaccga ggccggtgtt gcgcacgata ttgacccccg gggtctcggc
     1621 tgggaccaga aacatcgaca tgccctggta cgcgctcacg tcggggtcgg tcacggccat
     1681 gacgatgagg aatgacgccg tcttggcatt ggacgaaaac cacttgcggc cgttgatgac
     1741 ccattcgtcg ccgtcgcgaa ccgcacgggt cgtgaacagg gtcggatctg ctcccccgcc
     1801 cggttcggtc atcgaataac aagagaagac ctcaccgttg agtagcggtt ccaggtaacg
     1861 ccgcttctgc tcatcggtgc cgtagtgcgc caggatttcg gcattaccgg tgtcgggagc
     1921 ctgggtcccg aacacgatcg gcgcccaact cgaccggccg atgatttcgt tcatcagcgc
     1981 caacttcacc tgtccatatc cttggccgcc caggtctggc cccaggtggc acgcccacag
     2041 cccccggtcg cgcacctgct gcttcggcgc ctcgacaccg cccgaccgct ggcgcaacgc
     2101 gcccgcgacg cagcgttgcg gctgagcatc gagttgacct acaccgtctc ggtctccgaa
     2161 cgcgtcggca ccatggtggt gccgacgttc ttcggtacgg ccgacgaccg cgacgagcga
     2221 ctcactccgc tcaccgccgc ccccttcgtc gccccgttcg gacctgcctt tgccgcctgg
     2281 tcaaccgagg aagaccagcg ggcctggatc gaacacggcg gcgacgaaga ctccgacctc
     2341 aacgcacgct acgaacgcat cctcgcagag actccgaagc ggggttacag catcgagcgc
     2401 atgtcctccg ccctgcgcca agcggcacag gttctcgtcg cgaacgagca ggaccggtgg
     2461 tcgcgcccga cacggcgcgg tcgcactcaa catcggagtg cacccctttg ccgacttgga
     2521 cgatgcggcg atcgaagaca tcggcgatcg cctgcgcgac atcactgcgc aggcgagtca
     2581 ctgattcggc gagcggggag tcgctgggcc gccaccgcgg tcacaggccg tagatcacac
     2641 cgcctcgaag ggtgtcgccg aacaggagat acatgatttg gttcggcaac ggagctggaa
     2701 tgaacgtcac ctgatacgac accgcgcccg tcacctcgaa atcctcctcc gcggcggcca
     2761 ggtcgatccc ta
//
LOCUS       DAOOMA010000364         2770 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_44737_length_2770_cov_4.579006, whole genome shotgun sequence.
ACCESSION   DAOOMA010000364 DAOOMA010000000
VERSION     DAOOMA010000364.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 2770)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2770)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2770
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44737_length_2770_cov_4.579006"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1380
                     /locus_tag="PLP95_09690"
     CDS             <1..1380
                     /locus_tag="PLP95_09690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012224888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HPB46112.1"
                     /translation="DKAIDLIDEAGARVRIRRLQSPPDYRELEAEIAEVTRQKKEAVE
                     KQDFEEAGRLRDKEKELLARKDAKDAEMKASGVDLFDEVDEEAIAEVLSLWTGIPVYK
                     LTEEETAKLLRMEDELHKRVIGQHEAIKAVSQAIRRTRAGLKDPRRPSGSFIFLGPSG
                     VGKTELAKTLAEFLFGDESAMITLDMSEYMEKHTVSRLVGSPPGYVGYDEGGQLTEAV
                     RRKPFSIVLFDEIEKAHPDVFNTLLQILEEGRLTDSQGRSVDFRNTVLIMTSNLGTAD
                     LRKAVVGFSKRDEAVSYEKMKAKVNEALKAHFRPEFLNRIDETIVFGELSKPEVIQIV
                     DFMVARVAEQLSGQGMGLEVSDPAKLLLADRGYDPTMGARPLRRAIQRLVEDPLSEKL
                     LWKEFRAGQIISIDVADDPENPGDQILTFAGKEGFIPPTLEADDLLEVAPPAPPAVTA
                     AGGDSAAEA"
     gene            1580..2383
                     /locus_tag="PLP95_09695"
     CDS             1580..2383
                     /locus_tag="PLP95_09695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB46113.1"
                     /translation="MIALRRIRSTLVAVVVGAMTVLSSACQAIATVAIDAELDGSGTV
                     TVTVDLDASAAGQLGDPETAFAVDDLRDAGWTIDGPNAEGEHTTLAASKPFANADDLP
                     RVIAEVSGPDGPFQDVTLTHANGFAGSSLELSGRWKLTGSLDQFSDNEVAAQLDGYPL
                     GRTPEEIAAALQEHPEALKLVLEVSMPGSVQASERFTLTDGTAASGEVLGTGAPIDEA
                     FSVSTRSNNRSVVAWMIGAAVLMVGGLVVMVAALRRPQRRRGSNSTDGF"
     gene            2373..>2770
                     /locus_tag="PLP95_09700"
     CDS             2373..>2770
                     /locus_tag="PLP95_09700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB46114.1"
                     /translation="MGSDPTGSEPVACNAPRGANGFGPIRDAVTLALLGVPIGLVVGM
                     ASAAFLASLEWATGVQHRHGWLLWFLPLGGAAVAMIQTRFAAGSSGGLTSALRRMRGE
                     DVDVAATTAPVVFGGSTVSHLLGASAGREGA"
BASE COUNT          463 a          906 c          927 g          474 t
ORIGIN      
        1 gacaaggcca tcgacctgat cgacgaggca ggtgcccgtg tgcgcattcg tcggcttcag
       61 agcccgcccg actaccgcga actcgaggcc gagatcgccg aggtcacccg gcagaagaag
      121 gaagccgtcg agaagcagga tttcgaagag gccggtcgcc tgcgagacaa ggaaaaggaa
      181 ctcctcgccc gcaaggacgc gaaggatgcc gagatgaagg cgtccggagt cgacctgttc
      241 gacgaggtcg acgaggaggc catcgccgag gtgttgtctc tgtggaccgg cattccggtt
      301 tacaagctca ccgaggagga gacagcgaag ctcctgcgca tggaagatga gctgcacaag
      361 cgggtcatcg gccaacacga ggccatcaag gccgtcagcc aggcgattcg ccgcactcgc
      421 gccggactca aggatcctcg tcgtcccagc ggttcgttca tcttcctcgg cccctcgggt
      481 gtcggtaaga ccgagctcgc caagacgctc gcggagttcc tcttcggcga tgagtcggcg
      541 atgatcaccc tcgacatgtc cgagtacatg gaaaagcaca cggtgagccg tctcgtcggt
      601 tcgcctcccg gctacgtcgg ctacgacgag ggcggtcagc tcaccgaggc cgttcgccgt
      661 aagccgttct cgatcgtgct gttcgacgag atcgaaaaag cccaccccga cgtgttcaac
      721 acccttctgc agatccttga ggaaggccgt cttaccgaca gccagggtcg ttcggtcgac
      781 ttccgcaaca cggtgctgat catgacctcg aacctcggca ccgccgatct tcgcaaggcc
      841 gtggtcgggt tctccaagcg cgacgaggcc gtctcctacg aaaagatgaa ggcgaaggtc
      901 aacgaggcgc tcaaggcgca cttccgcccc gagttcttga accgtatcga cgagaccatc
      961 gtgttcggcg aactgtccaa gcccgaggtc atccagatcg tcgatttcat ggtcgctcgc
     1021 gtggccgaac agctcagcgg tcaaggcatg ggcctcgagg tcagcgaccc ggccaagttg
     1081 ctcctcgccg accgcggcta cgacccgacg atgggcgctc gcccgcttcg tcgtgcgatt
     1141 cagcgcctcg tcgaggatcc gctgtcggag aagctcctgt ggaaggagtt ccgcgccggt
     1201 cagatcatca gcatcgacgt cgccgacgat ccggagaacc cgggcgatca gatcctcacc
     1261 ttcgcgggca aggaagggtt catcccgccc accctcgaag cggatgatct gctcgaggtg
     1321 gctccgccgg caccgccggc cgtcactgcc gccggcggag actcggccgc cgaagcctga
     1381 gtcgggccgc agcgctcttg gtcgagcctg cgaccctcaa cccgaatttc ccgagtctcg
     1441 acgacgtcgg gtgtgcacgg ccaaacggcc ccgcacaccc gacgtcgtcg cgtttcaggc
     1501 tcccgacgct cgccgggtgt catcgtgtct catcggcggt cgtcggaggc cgagaggctc
     1561 accggtaggc tgtgatgccg tgattgccct gcgccggatc cgctcgaccc tcgtcgccgt
     1621 cgtcgtgggg gccatgacgg tgttgagttc cgcctgccag gccatcgcaa cggtggcgat
     1681 cgatgctgaa ctcgacggtt caggcacggt caccgtcacg gtcgatctcg acgcttcggc
     1741 ggccggccag ctcggggatc cggagaccgc gtttgccgtc gacgaccttc gcgacgccgg
     1801 ctggacgatc gacggtccga acgcggaagg tgaacacacc accctcgccg cttcgaagcc
     1861 gttcgccaat gccgacgacc ttccccgggt catcgccgag gtctccggcc cggacgggcc
     1921 gttccaggac gtgacgctga cccacgccaa cggctttgcc ggttcctccc tggaactgag
     1981 cggccggtgg aagctcacgg ggtcgctcga tcagttcagc gacaacgaag tcgccgccca
     2041 actcgacggc tatccgctcg gacgaacccc cgaagagatc gctgcggcgc tgcaggaaca
     2101 cccggaggcg ctgaagttag tgctcgaggt gtcgatgccc ggaagcgttc aggcctccga
     2161 gcggttcacg ctgaccgacg gcacggcggc gagcggtgag gtgttgggca ccggcgcgcc
     2221 catcgacgag gcgttctcgg tgtcgacgcg gtcgaacaac cggtcggtgg tggcctggat
     2281 gatcggcgcg gcagtgttga tggtcggcgg tcttgtggtc atggtggctg cgctgcggcg
     2341 tccacaacgc cgtcgtggct ccaatagcac cgatgggttc tgacccgacc ggttctgaac
     2401 cggtcgcctg caacgcccct cggggggcga acggattcgg gccgattcga gatgcggtca
     2461 ccctggcgct cctcggcgtg ccgatcgggt tggtcgtcgg catggcgagc gcggcgtttc
     2521 tggcgtcgct ggagtgggcg accggcgttc agcaccgcca cgggtggctg ctgtggttcc
     2581 tgccgcttgg cggagcggcg gtggcgatga tccagactcg attcgctgcg gggtcgtccg
     2641 gcgggttgac gagcgcgctg cgccgtatgc gcggcgagga cgtcgatgtg gcggcgacga
     2701 cggctccggt cgtgttcggc ggatccacgg tctcccatct cctcggtgca tccgccggac
     2761 gcgagggggc
//
LOCUS       DAOOMA010000365         2769 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_44761_length_2769_cov_3.922623, whole genome shotgun sequence.
ACCESSION   DAOOMA010000365 DAOOMA010000000
VERSION     DAOOMA010000365.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 2769)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2769)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2769
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44761_length_2769_cov_3.922623"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..235)
                     /locus_tag="PLP95_09705"
     CDS             complement(<1..235)
                     /locus_tag="PLP95_09705"
                     /inference="COORDINATES: protein motif:HMM:NF024900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HPB46115.1"
                     /translation="MPEAPKPITIDQPVTITINGDAVIAQKGDLLIDAAERAGHYIPR
                     FCYHSRMNPVGMCRMCLVEVDTGRGPALQPACML"
     gene            complement(228..1610)
                     /gene="nuoF"
                     /locus_tag="PLP95_09710"
     CDS             complement(228..1610)
                     /gene="nuoF"
                     /locus_tag="PLP95_09710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015439973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HPB46116.1"
                     /translation="MSDGNVPQVVSSRWDVLDAHTLEGYKRSGSYEGYSGIAKALTRR
                     PSEVLEEVRDATVLGRGGAGFPAGTKWGFTPAGVWPRYIVVNGDESEPGTYKDRVLME
                     RDPHQLIEGCLIAAYAIGAAQVFLYVRGEMAHAQERVAAALNEAYAAGLVGKNIMGTD
                     FSVDIVLHWGAGAYIVGEETALIESLEGNRGMPRLKPPYFPAAKGLYMKPTIVNNVET
                     LSNLPWLMNHGAEVYKQTGSESSPGTRLVAVSGHVNNPGVWEVPQGTTTFRELFYSEQ
                     YGNGIRNDNALKAFIPGGASAPWFFPEDLDLPLEGRTIGAAGSMAGSGAIVVMDETVD
                     AVKACLRIVRFYARESCGKCSPCREGTAWEEKILQRIVDGYGRPSDIDLLLDVADNIS
                     PGPYPVAADPNQGLEAVPYPPKQTTICPLGPSSVAPITSALRRFRHEFEAKITRREPI
                     PVSAAGGHDA"
     gene            complement(1607..2233)
                     /locus_tag="PLP95_09715"
     CDS             complement(1607..2233)
                     /locus_tag="PLP95_09715"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020379278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HPB46117.1"
                     /translation="MSRFTEANEQLAREIIARYPRAKSATIPLCHLAQEQDGYLADDA
                     ISHIAELVGITSAEVLGTASFYEMFKRKPVGKYLVNVCTNISCQVMGAEELLAHAEEK
                     LGVPAGGTTDDGMFTLEDVECIAACTEAPACLVNYRFQHRVTNESFDTLIDDIRAGKA
                     TEIPLHGTLAKARQHIDPSQVANIAPPEQQVEPAWFAARNATGEGDAS"
     gene            complement(2230..>2769)
                     /locus_tag="PLP95_09720"
     CDS             complement(2230..>2769)
                     /locus_tag="PLP95_09720"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015797835.1"
                     /note="Catalyzes the transfer of electrons from NADH to
                     quinone; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit D"
                     /protein_id="HPB46118.1"
                     /translation="ALSVTGPILRSTGYAWDLRRDAPYLRYDEVDFDVVVGGHGDTFD
                     RYAIRLAEIEESIRILRQVIEKMPEGDYRVQDKKVTPPPRARIDESMEALIHHFKLYT
                     EGFKVPEGEAYVAVESPRGELGCYIVSDGSAKPYRMHIRGPSFSNLQSLPHLMRDSYL
                     ADTVAIVSSVDPIMGEVDR"
BASE COUNT          476 a          932 c          887 g          474 t
ORIGIN      
        1 ccaacatgca cgccggctgc aacgcgggtc cgcgaccggt gtcgacctcg acgaggcaca
       61 tgcggcacat gcccaccggg ttcatgcggc tgtggtagca gaaccggggg atgtaatgac
      121 cggcacgctc ggcggcgtcg atcagcagat cgcccttctg cgcgatcacc gcgtcgccgt
      181 tgatcgtgat ggtgaccggc tggtcgatgg tgatgggctt cggtgcctca ggcatcgtgg
      241 cctcccgcgg cgctcaccgg aatcggctca cgcctggtga tcttcgcctc gaactcgtga
      301 cggaaccggc gcagcgcgga cgtgatcggc gcgaccgacg acggaccgag cgggcagatg
      361 gtggtttgct tgggcggata cgggaccgcc tcgaggccct gattcggatc cgcggccacc
      421 ggatagggcc cgggcgaaat gttgtcggcg acgtccaaca gcaggtcgat atcgctcgga
      481 cggccgtagc cgtcgacgat gcgctggagg atcttctcct cccaggccgt gccctcacga
      541 cacggcgagc acttgccgca cgactcgcgg gcgtagaacc gcacgatgcg caggcaggcc
      601 ttcaccgcgt cgaccgtttc gtccatcacc acgatggcgc ccgaaccggc catggagccg
      661 gccgcgccga tcgtgcggcc ctcgagcgga aggtcgaggt cctccgggaa gaaccacggg
      721 gcagacgcgc cgccggggat gaacgccttg agcgcgttgt cgttgcggat gccgtttccg
      781 tactgctcgg aatagaacag ctcgcgaaac gtcgtcgtgc cctgaggcac ctcccacacg
      841 ccggggttgt tcacgtgacc cgacaccgcg acgaggcggg taccgggcga cgactccgaa
      901 ccggtctgct tgtagacctc ggcgccgtgg ttcatcagcc atggcagatt cgacagcgtc
      961 tcgacgttgt tgacgatcgt cggcttcatg taaaggccct tggccgccgg gaaatacgga
     1021 ggcttgagcc gcggcatgcc gcggttgccc tcgagcgact cgatgagcgc cgtctcctcg
     1081 ccgacgatgt aggcccccgc accccagtgc aacacgatgt cgacactgaa gtcggtgccc
     1141 atgatgttct tgccgaccaa gcccgccgcg tacgcctcgt tcagcgcggc cgccacccgt
     1201 tcctgggcat gggccatctc gccgcggaca tacaagaaca cctgcgccgc gccgatcgcg
     1261 taggccgcga tcaggcagcc ttcgatgagc tgatgcgggt cgcgctccat gagcacgcgg
     1321 tccttgtagg tgcccggctc ggactcgtcg ccgttcacca cgatgtagcg aggccacacg
     1381 cccgcagggg tgaaccccca cttggttccc gcggggaaac cggcgccgcc gcgacccagc
     1441 accgtcgcgt cgcggacctc ttcgaggacc tccgatggcc gtcgggtcaa cgccttggcg
     1501 ataccgctgt agccctcata gctgccggag cgcttgtagc cctcgagcgt gtgggcgtcg
     1561 agcacatccc aacgggagga cacgacctgg gggacgttgc cgtcgctcat gatgcgtctc
     1621 cttcgccggt cgcattgcgg gccgcgaacc acgccggctc cacctgctgt tcgggcgggg
     1681 cgatgttggc cacctgcgac gggtcgatgt gctggcgagc cttcgccaac gtgccgtgca
     1741 acggaatctc ggtcgccttg ccggcgcgga tgtcgtcgat cagcgtgtcg aaggactcgt
     1801 tggtgacccg atgctggaac cggtagttca ccaggcacgc cggggcctcg gtgcacgccg
     1861 cgatgcactc gacgtcttcc agcgtgaaca tgccgtcgtc ggtggtgccc ccggcgggga
     1921 cgcccaattt ctcctcggcg tgcgccaaca gttcctcggc gcccatcacc tgacaggaga
     1981 tgttcgtgca cacgttgacc agatacttgc cgaccggctt gcgcttgaac atctcgtaga
     2041 acgacgcggt gcccaacacc tcggccgacg tgatgcccac caactcggcg atgtgcgaaa
     2101 tcgcatcgtc ggccagatag ccgtcctgtt cctgcgcgag atgacacagc ggaatcgtcg
     2161 ccgacttcgc tcgggggtag cgggcgatga tctcgcgagc gagctgttcg ttcgcctccg
     2221 tgaagcgact catcggtcca cctctcccat gatcggatcc accgacgaca cgatcgcgac
     2281 cgtgtcggcc agatagctgt cgcgcatcag atggggcagc gactgaaggt tcgaaaaact
     2341 cgggccgcga atgtgcatgc gatacggctt cgccgaaccg tccgacacga tgtagcagcc
     2401 aagctcgccg cgcggcgact cgaccgccac ataggcctca ccctcgggaa ccttgaaacc
     2461 ctcggtgtag agcttgaaat ggtgaatcaa cgcctccatc gactcgtcga tgcgggcccg
     2521 cggcggcggg gtcaccttct tgtcctgaac gcgataatcg ccctcgggca tcttttcgat
     2581 cacctggcgc agaatgcgaa tggattcctc gatctcagcc aaacgaatcg catagcggtc
     2641 gaaggtgtcg ccatgaccgc cgaccaccac gtcgaaatcc acctcgtcgt agcgcaggta
     2701 cggcgcatcg cggcgaagat cccaggcgta acccgtcgaa cgaaggatcg ggccggtcac
     2761 cgacagcgc
//
LOCUS       DAOOMA010000366         2768 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_44786_length_2768_cov_4.188721, whole genome shotgun sequence.
ACCESSION   DAOOMA010000366 DAOOMA010000000
VERSION     DAOOMA010000366.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 2768)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2768)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2768
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44786_length_2768_cov_4.188721"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1339)
                     /locus_tag="PLP95_09725"
     CDS             complement(<1..1339)
                     /locus_tag="PLP95_09725"
                     /inference="COORDINATES: protein motif:HMM:NF015013.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid II flippase MurJ"
                     /protein_id="HPB46119.1"
                     /translation="MTDAMSSVGASDASSESGSPQPPRRASVGRDSLWVAAGTTLSRV
                     TGFARVFVLAWAIGQSDLADVYALANTAPNLIYEFVVGGILSATLVPLFVRARERDDR
                     DAASVLMSVGFVAAIALTLLAMGLTFGVDRWATARWEDLDGQVSSWTQFQSGMAMLYL
                     FLPQILFYAITSLTTAYLNASRRYKTAAFAPVLTNVVSIIAFVIVGFRLQGRDDILDS
                     TIFDSSTIYWLGIGTTAGIFAMTIPLLWEMRRLHNPLRFVPRFRHPIVSTLIRLSGWT
                     FGYVAANQVALLFVATTVTSLSRYQNAFIFFQLPHGLIAVSLMTTITPELASAVAATD
                     YQALADRFVRGLRLLASLLLPAAVGYALLAVPIASFLARGSLTAEDARLTGTTLAAFA
                     IGLPAFSAYLYACRGFYAMSNTRTPFFLNLLENGANIAFVIVLSVSGRSSSAWF"
     gene            complement(1336..2064)
                     /locus_tag="PLP95_09730"
     CDS             complement(1336..2064)
                     /locus_tag="PLP95_09730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB46120.1"
                     /translation="MPTSGPPPDSIPNASVLATPMTLGAAISGAAIGALIGMLAGPVG
                     AVLLAVIGALIGVAIRLLSARSPQERAGGIDPFTVQEPWRRLTQDAQRTRTTFRDALS
                     RTPAGPIHDRLDTIGSRVDDAVANVWRTAQAGHELAAALYKMPAESDSAARVRDQVEV
                     TEQRLRRANDELTETLALAIELSVSANAPDEFNSIEAGVSAISDELGAVYSALQETSS
                     LSRRPGPSSTDPPTEPGTGTGSAP"
     gene            complement(2103..>2768)
                     /locus_tag="PLP95_09735"
     CDS             complement(2103..>2768)
                     /locus_tag="PLP95_09735"
                     /inference="COORDINATES: protein motif:HMM:NF012234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HPB46121.1"
                     /translation="ETDRSGLVAGYVGQTAPLVTRRFDEADEGMLFIDEAYTLVRGGE
                     NDFGREAIDQLVKLIEDRRDRTAVVVAGYPEEMEAFIEANPGLRSRFPKTIVFPDYET
                     SELIDIFERICDKQRYELSDEARTKLHDLINTTERGRGFGNGRFVRNVFEDAVVRHAT
                     RVARIEVPSDEDLQRFEPEDLTTAEDPTTETTTETTTYAGNDSTPAAAPPTEPTPTVN
                     DDG"
BASE COUNT          483 a          813 c         1005 g          467 t
ORIGIN      
        1 cgaaccacgc gctgctcgac cggccggaaa cggacaacac gatgacaaac gcgatgttgg
       61 cgccgttttc caacaggttg agaaagaatg gagtgcgggt gttgctcatc gcgtagaagc
      121 cgcggcaggc atacagatag gccgaaaatg ccggcaggcc gatggcgaac gctgcgagtg
      181 tcgtgccggt gaggcgggcg tcctcagcgg tgaggctgcc gcgtgcgaga aacgaggcga
      241 tgggaacggc gaggagcgcg tatcccaccg ccgccggcaa cagcagcgag gcgagtagtc
      301 gcaagccccg cacgaaccga tcggcgagcg cctggtagtc ggtggccgcg accgcgctgg
      361 cgagttccgg ggtgatggtg gtcatcaacg aaaccgcgat gaggccgtgg gggagttgga
      421 agaagatgaa tgcgttctga tagcgagaca gcgaggtgac ggtggttgca acaaacagca
      481 gggcgacctg gtttgccgcc acgtatccga aggtccaacc cgagagccgg atcagcgtcg
      541 aaacgatcgg gtggcgaaat cgtggaacaa agcgcagcgg gttgtggagg cgcctcatct
      601 cccacaagag cgggatcgtc atggcgaaaa tgccggcggt cgtgccgata ccgagccagt
      661 agatggtcga ggagtcgaag atcgtcgaat ccaagatgtc gtcgcggccc tgcaggcgaa
      721 atccgacgat gacgaacgcg atgatgctga cgacgttggt gagcaccggg gcgaatgcgg
      781 ccgtcttata gcggcgggag gcgttgaggt aggcggtcgt gagcgaggtg atggcgtaaa
      841 agaggatctg agggaggaac aggtacagca tcgccatgcc ggactggaac tgcgtccaac
      901 tcgacacctg cccgtcgagg tcctcccagc gagcggttgc ccaccggtcg acgccgaatg
      961 tgaggcccat cgccagcagg gtcagagcga tcgctgccac gaagccgacc gacatgagta
     1021 ccgaggcggc gtctcggtcg tcgcgttctc gggcgcgtac gaagagcggc acgagggttg
     1081 ccgacaaaat gccgccgacc acgaactcgt agatcaggtt cggggcggtg ttcgccagcg
     1141 cgtagacatc tgccagatcg gattgtccga tggcccacgc caacacgaag acccgtgcga
     1201 atccggtgac gcgactcagc gtcgtgccag cggcgaccca caacgagtcg cgtccgacac
     1261 ttgcccgcct cggcggctgg ggagacccgg attcgctcga tgcgtcggat gcgccgaccg
     1321 aactcatcgc gtcggtcatg gagccgagcc ggtcccggtg cctggctctg tgggcgggtc
     1381 ggtcgacgag gggcccggcc ggcggctgag cgaagacgtc tcctgcaagg cggagtacac
     1441 ggctccgagt tcgtcggaga tggccgacac cccggcttcg atcgagttga actcgtcggg
     1501 agcgttggcg ctcaccgaca attcgatcgc gagggcgagc gtctcggtga gttcatcgtt
     1561 tgctcgtcgc agccgctgtt cggtcacctc gacctggtcg cgcacgcgag cggcgctgtc
     1621 ggattctgcc ggcatcttgt acagggcggc tgccagctca tgtccggcct gggcggtgcg
     1681 ccacacgttg gcgacggcgt cgtcgacgcg ggatccgatc gtgtcgagac ggtcgtggat
     1741 cggccccgcc ggggtgcgcg aaagcgcgtc gcgaaacgtg gtgcgggtgc gttgggcatc
     1801 ctgggtgagg cgtcgccacg gttcctgcac cgtgaacgga tcgatccctc cggcccgctc
     1861 ttgggggctt cgtgcgctga gcagccggat ggcaacaccg ataagggcgc cgatcacggc
     1921 gagcagcacc gccccgaccg ggcccgccag catcccgatg agcgcaccga tggcggcgcc
     1981 ggagattgcg gcgccgaggg tcatcggagt cgccagaaca gacgcgttgg ggatcgagtc
     2041 gggcggaggg ccagaggtgg gcatggatgc gttggggatc ttgtggtgac accgagcggt
     2101 ggtcagccgt cgtcgttcac tgtaggcgtc ggctcggtgg gcggcgccgc tgcgggcgtc
     2161 gagtcgttac cggcgtaggt ggtggtttcg gtggtggttt cggtggtggg gtcctcggcg
     2221 gtggtcaggt cctccggttc gaagcgctgc aggtcctcgt cgctcggtac ctcgatccgg
     2281 gcgacccggg tggcgtggcg aaccaccgcg tcttcaaaga cgttgcgcac gaagcgtccg
     2341 ttgccgaatc cccgtccgcg ttcggtggtg ttgatcaggt cgtgcagctt cgttcgcgcc
     2401 tcatcggaga gctcgtagcg ctgtttgtca cagatccgct cgaagatgtc gatcagctcg
     2461 ctggtctcgt aatcgggaaa cacgatcgtc ttggggaacc tcgagcgcag gcccgggttg
     2521 gcttcgatga acgcttccat ctcctcgggg tagccggcga cgacgactgc ggtgcggtcg
     2581 cgccggtctt cgatcagctt gacgagttga tcgatcgcct cgcgaccgaa gtcgttctcg
     2641 ccaccgcgca ccagggtgta ggcctcgtcg atgaatagca tcccctcgtc ggcctcgtcg
     2701 aagcgtcggg tgaccagcgg agcggtctgg ccgacgtagc cggcgaccag acccgatcgg
     2761 tcggtctc
//
LOCUS       DAOOMA010000367         2762 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_44913_length_2762_cov_3.271149, whole genome shotgun sequence.
ACCESSION   DAOOMA010000367 DAOOMA010000000
VERSION     DAOOMA010000367.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 2762)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2762)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2762
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44913_length_2762_cov_3.271149"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..343)
                     /locus_tag="PLP95_09740"
     CDS             complement(<1..343)
                     /locus_tag="PLP95_09740"
                     /inference="COORDINATES: protein motif:HMM:NF012698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HPB46122.1"
                     /translation="MAGLEAGADDYLTKPFVPKELSARIRALLRRARAADTGVTRMRF
                     ADIEIVPQEGLVTKDGEQVHLTKTEFRLLVELAANAGRVFSREALLEKAWGHDYFGDG
                     CPVDVHIRRIRT"
     gene            complement(411..554)
                     /locus_tag="PLP95_09745"
                     /pseudo
     CDS             complement(411..554)
                     /locus_tag="PLP95_09745"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
     gene            complement(672..1517)
                     /locus_tag="PLP95_09750"
     CDS             complement(672..1517)
                     /locus_tag="PLP95_09750"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012673.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006020 - inositol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046855 - inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HPB46123.1"
                     /translation="MAHFDSDQPNPTLDLPADPRELATIALRIASSAADLVRERRAQG
                     FGIETKSTATDMVTDVDRASDRLIRELIGQARPDDGIITEEDATIVGTSGVTWVADPI
                     DGTTNFVYGMRPYLVSIGAVVDGVSVAAAVVEISGDTRFHAALGSGSYRDGARLRVPE
                     APTLDRALVATGFGYAPERRKRQARVVAGIIDHVRDIRRLGAAAYDLCAVAMGHVDGY
                     YEHGLGAWDLAAGRLIATEAGAAVEAIAGGPAEPHEGVIAAHPDLIGPLRSLIVEHGA
                     LEVLD"
     gene            complement(1561..1830)
                     /locus_tag="PLP95_09755"
     CDS             complement(1561..1830)
                     /locus_tag="PLP95_09755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB46124.1"
                     /translation="MTEPESALPSVRARAIAFASVLLCGLLGGAVGVWFMDTQCSDEC
                     TVPTGIAMWVGTVILALGAAVVAVLSLRTLAEWKSQGAAAEALRR"
     gene            1957..>2762
                     /locus_tag="PLP95_09760"
     CDS             1957..>2762
                     /locus_tag="PLP95_09760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB46125.1"
                     /translation="MKFDVDEHPGGGGITAASLVPPARLASLLSKARAAAGKTVEEVA
                     ANSDGRFTPAMLAAFESGIRTVTDEDAAALASLYALETTDIVPARSRLVLDTDTRHLF
                     VVGRTERIDLEAPSHDELLTRYLAMVYSMRGQAAGSNLVLRTEDLDVLGRALQRDRHD
                     VRVRLEALIAAPEELASWRHRLLNRRVLIPAAGVLVAAIAAGSLILISGNDGLPASSA
                     STMTSITSTAPTTSTAPTTIVAAEVPVDIGSAVVVERGADGSVGEARVRTG"
BASE COUNT          434 a          918 c          961 g          449 t
ORIGIN      
        1 tggtgcggat gcgccgtatg tgaacatcga cggggcagcc gtcgccaaag tagtcgtgac
       61 cccacgcctt ttcgagcagc gcctcacggc tgaacacccg accggcgttg gctgcgagtt
      121 cgaccaggag ccgaaactcg gtcttggtga ggtggacctg ttcgccgtcc ttggtgacga
      181 ggccctcctg aggcacgatc tcgatgtcgg cgaacctcat gcgcgtcacg ccggtgtcgg
      241 ccgcacgggc ccggcgcaac agtgctcgga tacgcgccga gagttctttg gggacgaacg
      301 gtttggtgag gtaatcgtcg gcccccgcct cgaggccggc cacgacgtcg tgggtcggct
      361 cgggcggtga ccatcacgat gggaacgtcg ctgacacgcc ggatcgcccg acacacatcg
      421 aacccgtcga taccgggcaa catgatgtcg atgagcacca catcggccgg ctgatgggag
      481 aactgggcga tggcatcctc gccgttggtg gcctccgcga cttcccaccc ttcatcttcg
      541 agggccagtt tcaccgacgt ccgaatccgc tcgtcatcct caacggtcaa aatgcgcgta
      601 cccacccgat cagtctttca tgcgccgcga gcaaaacggc gccacgcggc gttggttcgg
      661 cgacggccga ctcaatcgag gacctcgagt gcgccgtgtt cgacgatgag cgagcgcaag
      721 gggccgatca ggtcggggtg cgcggcgatg actccctcgt ggggctcggc cggacccccg
      781 gcgatggcct cgaccgcggc gccggcctcg gtggcgatga gccggccggc ggcgaggtcc
      841 cacgctccga gtccgtgttc gtagtagccg tcgacgtgtc ccatggcgac ggcgcacagg
      901 tcgtaggccg ccgcgcccag gcggcgaatg tctcggacgt gatcgatgat gccggcgacc
      961 acgcgcgcct gtcgtttgcg gcgttcgggt gcatacccga atccggtggc cacgagtgcc
     1021 cggtcgagcg tcggcgcttc gggcaccctc aatcgggcgc cgtcgcgata ggaccccgac
     1081 ccgagcgctg catgaaagcg cgtgtcgccg gagatctcga cgacggcggc cgcgaccgag
     1141 accccatcga cgacggcccc gatggatacg aggtagggcc gcatgccgta gacgaagttg
     1201 gtggtgccgt cgatgggatc ggcgacccag gtgacaccgc tggtgcccac gatggtcgcg
     1261 tcttcctcgg tgatgatccc gtcatcggga cgcgcctggc cgatcaactc ccggatgagc
     1321 cgatccgagg cccggtcgac gtcggtgacc atgtcggtgg cggtcgactt cgtctcgatt
     1381 ccgaacccct gagcccgccg ctcgcgtacg agatcggcgg cgctcgacgc gatccgcaac
     1441 gcgatcgtcg ccaattcccg tgggtcggcg ggcaggtcga gcgtggggtt gggttggtcg
     1501 gagtcgaaat gagccatgtt gggaccctat tggtgcggct gccctatcgg tgcggcggtc
     1561 ctacctgcgg agggcctcgg cggcggcacc ctgacttttc cattcggcga gggtgcgcag
     1621 gctcaacacg gcgaccacgg ccgcgccgag ggccaggatc acggtcccga cccacatggc
     1681 gatgccggtg ggcacggtgc actcgtcgct gcactgggtg tccatgaacc acacgccgac
     1741 cgcgccgccg agcaacccgc acaacaacac cgacgcaaag gcgatggcgc gagcgcgaac
     1801 cgagggcagg gcactttcgg gttcggtcat cagcgggtca tccacagggg tcaggttagg
     1861 tcggcgaaga atttgtttgt gtcaccgtgg tcccgcgccg atacagcgga ggccgatgca
     1921 ggttgccgac ggacgtaacg aaggaaggcc gtggtcatga aattcgatgt ggacgagcat
     1981 ccgggtggtg gggggatcac cgcagcgtcg ttggtgcctc cggcgcgcct ggcctcgctg
     2041 ctcagcaagg ctcgggccgc agcgggcaag acggtcgaag aggtcgcggc gaattccgac
     2101 ggtcgcttca ccccggcgat gctggcggcc tttgagtcgg gcatacgaac ggtcaccgac
     2161 gaggacgcgg ccgcgctcgc gtcgctctac gcgctcgaaa ccaccgacat cgtcccggcc
     2221 cgttctcgcc tggtgctcga caccgacacc cggcacctct tcgtggtcgg ccgcaccgag
     2281 cggatcgatc tcgaagcgcc cagccacgac gagttgctca cccggtatct ggccatggtc
     2341 tattccatgc gcgggcaggc cgccggctcg aacctcgtct tgcgcaccga ggacctcgac
     2401 gtgttggggc gcgcgttgca gcgcgaccga cacgacgtgc gggtacgtct cgaggcgctc
     2461 atcgcggctc cggaggagtt ggcgtcgtgg cgccaccgac tgctgaatcg gcgggtgttg
     2521 attccggccg ccggagtcct cgtcgccgcg atcgccgccg gcagcctgat cctgatcagc
     2581 ggcaacgacg ggctccccgc gtcctcggcc tccacgatga cgtcgatcac ctcgacggct
     2641 ccgacgacgt cgacggcccc gacgacgatc gtcgccgcgg aggttccggt cgacatcggc
     2701 tctgccgtgg tcgtcgagcg cggcgcggac ggcagcgtcg gcgaagcgag ggtgcggacc
     2761 gg
//
LOCUS       DAOOMA010000368         2756 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_45030_length_2756_cov_4.306183, whole genome shotgun sequence.
ACCESSION   DAOOMA010000368 DAOOMA010000000
VERSION     DAOOMA010000368.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 2756)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2756)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2756
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45030_length_2756_cov_4.306183"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1060
                     /locus_tag="PLP95_09765"
     CDS             <1..1060
                     /locus_tag="PLP95_09765"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HPB46126.1"
                     /translation="TVAAVASYWLHRAITYAAEPARRWYRDVRHYVAAATFAGLVDLA
                     VLLVVAGNSTAVLHLLIAKLAAVVVAFLIRLVSYRQTMFDVIRSDQGRDPERGPAPGE
                     VRLSLVIPAYCEEAGIAATVDRVEQALGHLRDEGGFELIVVDDGSPDDTSSAARDAGA
                     DVVITLERNQGKGAAVRAGMLAARGRSVAFTDADLSYSPDQVLRLLAALEEGWDVVVG
                     SRKHTSTLTLVETGRLREIGSRVVNALTMFVLLGQYRDTQCGLKAFRSDVARLVFSRT
                     RIDGFAFDIEVFHLCERFRLTLTEVPVEVANSERSTVHVARDTIRLIRDVFRIRSIAH
                     TGGYEIEPDEPTDFVTAN"
     gene            1088..2440
                     /gene="manB"
                     /locus_tag="PLP95_09770"
     CDS             1088..2440
                     /gene="manB"
                     /locus_tag="PLP95_09770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442410.1"
                     /note="converts mannose-6-phosphate to
                     mannose-1-phosphate; the resulting product is then
                     converted to GDP-mannose by ManC which is then used in the
                     synthesis of mannose-containing glycoconjugates that are
                     important for mediating entry into host cells; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HPB46127.1"
                     /translation="MNAFEAIFKAYDVRGIVPEQFDAEMARSIGAAFARFVLDDDPSV
                     TKVLVARDMRPSGHDMVAAFADGATGQGVDVVDLGLGSTDLLYYAAGSMHAPGAMFTA
                     SHNPAQYNGIKFCLSGARPVGEESGLFTVRDLAAAGLERVATPGTVSQRDVLADFAAH
                     VRSFIDPSVLKPLKVVADTANGMGGLVVPATFEGLPFDLEVMYGELDGTFPNHPADPI
                     QPENQRDLIARVLEVGADVGLAFDGDADRVFLVDELGVGLSGSTTTAILADAIVRRHG
                     GGKVVHNLICSKAVPEVIREAGGTPIRSRVGHSFIKGVMASEGAVFGGEHSAHYYFAD
                     NYRADSGLIAAVMVLEQLCLRDLPLSELRKPYERYAASGEINTKVPDTAEVIERVAVA
                     YEGEQQDRLDGLTVDLGDWWFNLRPSNTEPLLRLNLEAATPDDVARRVAEVRGHFGLD
                     "
     gene            2459..2686
                     /locus_tag="PLP95_09775"
     CDS             2459..2686
                     /locus_tag="PLP95_09775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trm112 family protein"
                     /protein_id="HPB46128.1"
                     /translation="MAISDDALLEFLACPQDKGPLLYLADEDLLYNPRLKVAYAIKEG
                     IPVMLASEATSVSEDEHQRIVAKAESAGAKS"
BASE COUNT          431 a          906 c          926 g          493 t
ORIGIN      
        1 caccgtggcc gcggtcgcgt cgtattggtt gcaccgcgcc atcacctatg cagcggagcc
       61 cgctcgccgc tggtaccgcg atgttcgcca ctacgtggcc gcggcgacgt tcgccgggtt
      121 ggtcgatctc gccgtgctcc tggtggtggc cggcaactcg accgcggtgc tgcacctgct
      181 gatagcgaag ctcgccgcgg tggtcgtggc gtttcttatc cgtctggtgt cgtaccgcca
      241 gaccatgttc gacgtgatcc gatccgacca ggggcgcgac cccgaacgcg ggcccgctcc
      301 cggcgaggtg cgcctcagcc tcgtcattcc tgcctactgc gaggaggcgg gcatcgcggc
      361 gacggtggat cgggtcgaac aagcgctcgg tcacctgcgc gacgaaggcg gtttcgaact
      421 catcgtcgtc gatgacggct cccccgacga cacttcgagc gcggcacgag acgccggggc
      481 cgacgtcgtg atcaccctcg agcgcaacca gggcaaaggg gcggcggttc gtgcgggaat
      541 gctcgcggcg cggggccgat cggtggcctt caccgacgcc gatctcagct actcacccga
      601 ccaggtgcta cggctgctcg ccgcgctcga ggagggttgg gatgtcgttg tcgggtcgcg
      661 caaacacacc agcacgctga ccctggtgga gaccggtcgg cttcgcgaga tcggcagccg
      721 ggtcgtgaac gccctgacga tgttcgtgct gttggggcag taccgagaca cccagtgcgg
      781 gttgaaggcg tttcgttccg acgtcgcccg actggtgttc agcaggacgc gcatcgatgg
      841 gttcgcgttc gacatcgagg tgtttcacct ctgtgagcgt ttccgcctca cgctgaccga
      901 ggtgccggtc gaggtggcga attcggagcg ctcgaccgtc cacgtggctc gcgacaccat
      961 tcggttgatt cgcgacgtct ttcgtatccg ctcgatcgcc cacaccggcg gatatgagat
     1021 cgagccggac gaaccgacgg acttcgttac cgcaaactga gccggcgacc ctacagtggc
     1081 catcgtcatg aatgcattcg aagcgatttt caaggcctac gacgtgcggg gcatcgtgcc
     1141 cgagcagttc gatgcggaga tggcccgctc catcggcgcg gccttcgccc ggttcgtcct
     1201 cgacgacgac ccgtcggtga ccaaggtgct ggtggcgcga gatatgcgcc cgtccggcca
     1261 cgacatggtc gcggcgttcg ccgatggtgc caccggccag ggcgtcgacg tcgtggatct
     1321 gggtctcggt tcgacagacc tgctctatta cgccgcaggc tccatgcatg ctcccggcgc
     1381 gatgttcacc gcatcgcaca acccggcgca gtacaacggc atcaagttct gtctgtcggg
     1441 agcccgtccg gtgggcgagg agtcggggct gttcacggtt cgcgaccttg cggcggccgg
     1501 actcgaacgc gtcgccacgc cgggaacggt gtcgcaacgc gacgtgctcg ccgacttcgc
     1561 ggcgcacgtg cgcagcttca tcgacccgtc ggtgctgaag ccgctcaagg tcgtggccga
     1621 cacggcgaac ggcatgggcg gcctggtcgt gccggcaacc ttcgagggtc ttccgttcga
     1681 ccttgaggtg atgtacggcg aactcgacgg gacgttcccg aaccatccgg ccgacccgat
     1741 ccagccggag aaccagcgcg atctgatcgc ccgggtgctc gaggtcggcg ccgacgtcgg
     1801 gctcgcgttc gacggtgatg ccgaccgggt gttcctcgtc gacgaactcg gcgtgggcct
     1861 gtcgggctcg accaccacgg cgatccttgc ggacgcgatc gtgcggcgcc acggcggcgg
     1921 caaggtcgtg cacaacctga tctgttccaa ggcggtgccc gaggtcatcc gcgaagccgg
     1981 cggaaccccg atccgcagtc gagtgggcca cagcttcatc aaaggggtca tggcctcgga
     2041 gggagcggtg ttcggcggcg aacactcggc gcactattac tttgccgaca actaccgcgc
     2101 cgattccggt ctcatcgcgg cggtgatggt gctcgagcag ttgtgccttc gcgaccttcc
     2161 gctgtcggag ttgcgcaagc cctacgagcg atatgccgcc tcgggggaga tcaacaccaa
     2221 ggtgcccgac accgccgagg tgatcgaacg ggttgctgtg gcctatgagg gtgagcaaca
     2281 agaccgcctc gacgggctca ccgtcgacct cggcgactgg tggttcaacc tgcggccgtc
     2341 caacaccgaa ccgctgctgc gcctgaacct cgaggccgcc acacccgacg acgtcgctcg
     2401 ccgtgtcgcc gaggtccgag ggcatttcgg cctcgactga tccggctacg ctcggttcat
     2461 ggccatctcc gacgacgccc tcctcgagtt tctcgcctgt ccgcaggaca agggtccgct
     2521 gctgtatctc gcggatgagg atctgctcta caaccctcgc ctgaaggtcg cgtacgcgat
     2581 caaggagggc attccggtga tgttggcctc ggaggcgacc tcggtgagcg aggacgagca
     2641 tcagcgcatc gtcgccaaag ccgagtccgc cggcgccaag tcctgagccg atcatcgtga
     2701 gtatcgacac cgactcggcg ggggaggtcc tggactcgat gggcgccatg gcgtcg
//
LOCUS       DAOOMA010000369         2748 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_45209_length_2748_cov_4.549944, whole genome shotgun sequence.
ACCESSION   DAOOMA010000369 DAOOMA010000000
VERSION     DAOOMA010000369.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 2748)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2748)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2748
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45209_length_2748_cov_4.549944"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..662
                     /locus_tag="PLP95_09780"
     CDS             <1..662
                     /locus_tag="PLP95_09780"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HPB46129.1"
                     /translation="AGFRAKSSAEPESEGERWSGVVISRFLVLALTMVLLNVFAAPVQ
                     QMQTDYLSNTRQMGPALIALFMLATNSWGFVGVALGARMADRSSRRLATAIGMVGFAI
                     GNTLLFTFGGPLMWVGSLIGSAVGGLIVPSLGALLPELFPTLRRGAANGLMNGAAVCG
                     SILGLLIVARSVSDGHYAPTVAMLALGPLIVAALVWALPETAGVELEELNPSDSAPDP
                     A"
     gene            complement(674..1414)
                     /locus_tag="PLP95_09785"
     CDS             complement(674..1414)
                     /locus_tag="PLP95_09785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB46130.1"
                     /translation="MPLASIEAYLSRPIAPTRRIALGDLHLPIDTAPGSGGVLLAGIM
                     ARFSRELDADTDEALSVLLDLLESGVRVAQPQLRHRMQVDRVGLMKARYHLESEDGRF
                     RFRFDSRVGSATQHVLAAAYAGATLSGTDRAAAFAAMRKGLDWGGAVDDRFVRFVADR
                     RHLGATVGSDPVGWAMTVLGIERAAPSDSESDDTGAATAREQFHIAITGAFRRQLIAA
                     HPDQGGDPERAADRIADIREARRILLSR"
     gene            complement(1508..2080)
                     /locus_tag="PLP95_09790"
     CDS             complement(1508..2080)
                     /locus_tag="PLP95_09790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB46131.1"
                     /translation="MVRLEDEDRVEPLLDEVNQSNDFGLDESKTRVVYEVGAWPAALQ
                     SSLAEALGIAEIPFEWDDDGDLVIYEDDEERVEEILDAMPDPDDPDAVDSDGIDVQAV
                     LSNLWESTRTLSKKATDADATVTFLDGAERMSRLSLPFGFDGAVWRWIVAEVLELRDA
                     MESDRATDVVTDDEVSDMAATISRSLRKFV"
     gene            complement(2096..2374)
                     /locus_tag="PLP95_09795"
     CDS             complement(2096..2374)
                     /locus_tag="PLP95_09795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB46132.1"
                     /translation="MSERDESETDETDETDAMDTSDAAGDLDTGDETDESGDEVIHSD
                     AELAAAEAEGDTIAEEFEEYVEYEMIGWSGESRTTLASMLTLNGIPHT"
     gene            2443..>2748
                     /locus_tag="PLP95_09800"
     CDS             2443..>2748
                     /locus_tag="PLP95_09800"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015799183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphatase class II"
                     /protein_id="HPB46133.1"
                     /translation="MTETTIETTAPDRNLAMELVRVTEAAAMAASPWVGAGDKNAADG
                     AAVDAMRKLLTTVAMRGTVIIGEGEKDEAPMLYNGEVVEHRRFVLFAFANDDGAAHGD
                     "
BASE COUNT          441 a          944 c          890 g          473 t
ORIGIN      
        1 cagccggctt ccgcgccaag tccagcgccg agcctgagtc cgaaggtgaa cgctggagcg
       61 gcgttgtcat ctcgcggttc ctcgtcttgg cactcaccat ggttctgttg aacgtcttcg
      121 ccgcgccggt ccaacagatg cagaccgact acctgtcgaa cacccgacag atgggccccg
      181 cactcatcgc gctgttcatg ttggccacga acagctgggg attcgtcggt gtcgccctcg
      241 gtgcacggat ggccgaccga tcgagtcgac ggctggcgac cgcgatcggc atggtcggct
      301 ttgccatcgg caacaccttg ttgttcacgt tcggaggccc cctcatgtgg gtcgggtcgc
      361 tcatcggctc ggcggtcgga ggtctcatcg tgccctccct cggggcactg ctccccgaac
      421 tgttcccgac ccttcgccgc ggcgcggcca acgggctgat gaacggtgct gctgtgtgcg
      481 ggagcatcct ggggttgttg atcgtcgctc gaagtgtctc ggacgggcac tacgcgccga
      541 ccgtcgcgat gctcgcactc ggaccgctga tcgtcgccgc actggtgtgg gcacttcccg
      601 agaccgccgg ggtcgaactc gaggagctca atccgagcga ttcggccccc gaccccgcgt
      661 gaccgcaccg gggtcatctc gacaacagga tgcggcgcgc ctcgcgaatg tcggcgatgc
      721 ggtcggccgc ccgctcgggg tctccgccct gatcggggtg tgcggcgatg agctgacgac
      781 gaaacgcacc ggtgatggcg atatggaact gttcgcgagc tgtcgctgca cctgtgtcgt
      841 cggattcgct gtcggacggc gccgctcgtt cgatgcccag caccgtcatc gcccagccga
      901 ccggatcgct tccgacggtg gcgccgaggt gacgccggtc tgccacgaaa cggacgaacc
      961 ggtcatcgac ggcgccgccc cagtcgaggc ccttgcgcat cgcggcgaag gccgcggcgc
     1021 ggtcggttcc gctcagcgtc gccccggcgt acgcggccgc cagcacgtgt tgggtcgcag
     1081 agccgacacg gctgtcgaac cggaaccgga accggccgtc ctcggattcc aggtgatagc
     1141 gagccttcat cagcccgacc cgatcgacct gcatccgatg gcgaagctgc ggttgagcga
     1201 cccgcacacc cgactcgagc agatcgagca gcacgctcaa ggcctcgtcg gtatctgcgt
     1261 cgagttcgcg tgagaaccgg gccatgatcc cggcgagcag gacaccgccg gatccgggag
     1321 ccgtgtcgat cggcaggtgg agatcgccaa gggcgattcg gcgggtcggg gcgatcggac
     1381 gcgacaggta ggcctcgata ctggccaacg gcattcggcc aaactacccg cacgatccga
     1441 ccgcggtagc accgggctga gaccgcggcc aggctccggc acggcgagcg cccggcgcgg
     1501 cgatcgacta gacgaatttg cgcagtgacc ggctgatcgt ggcggccatg tcggagacct
     1561 cgtcgtcggt caccacgtcg gtcgctcgat cggactccat ggcgtcgcgc agctccagca
     1621 cctcggccac gatccagcgc cacacggcac cgtcgaaacc gaacgggagg ctcaggcgcg
     1681 acatgcgttc ggccccatcg agaaaggtca cagtcgcatc cgcgtcggtc gccttcttgg
     1741 acagggttcg ggtcgactcc cacagattgc tcaacaccgc ctggacgtcg atcccatcgg
     1801 agtccaccgc gtcggggtcg tccgggtcgg gcatggcatc gaggatctcc tcgacacgtt
     1861 cctcgtcgtc ttcgtagata acgaggtcgc cgtcgtcatc ccactcgaag ggaatctccg
     1921 cgattccgag cgcctcggca agcgaactct gcagcgctgc gggccatgcg cccacctcgt
     1981 agacgacccg ggtcttcgac tcgtcgagac cgaaatcgtt cgactggttc acctcgtcga
     2041 gcaacggctc gacgcggtcc tcgtcctcga ggcgcaccac cagcgtcgct ccctgtcagg
     2101 tgtggggaat gccgttgagg gtgagcatcg aagccagggt ggtacgggac tcgcccgacc
     2161 aaccgatcat ctcgtactcg acgtactcct cgaactcctc ggcgatcgtg tcgccctcgg
     2221 cctcggccgc cgcgagttcc gcatcggagt ggatgacctc gtcgcccgac tcgtcggtct
     2281 cgtcgccggt gtcgaggtcc ccggcggcgt cgctcgtgtc catcgcgtcg gtctcgtcgg
     2341 tctcgtcggt ctcggattcg tctcgttcgc tcacaggcga catccttaga cgcgtccgcc
     2401 ccgattcggc caactccgta catgctccgg taccgtggaa ccatgactga gaccacgatt
     2461 gagaccactg cgccagaccg caacctggcc atggaactgg tccgcgtgac cgaggcggcc
     2521 gccatggcgg cgtcaccctg ggtcggggcg ggagacaaga acgctgctga cggcgccgcc
     2581 gtcgacgcga tgcgcaaact actcaccacc gtcgccatgc gcggcaccgt catcatcggc
     2641 gaaggcgaaa aggacgaagc gccgatgctc tacaacggcg aagtggtaga gcatcggcgc
     2701 ttcgtccttt tcgccttcgc caatgatgac ggtgccgcgc atggcgac
//
LOCUS       DAOOMA010000370         2744 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_45302_length_2744_cov_3.944217, whole genome shotgun sequence.
ACCESSION   DAOOMA010000370 DAOOMA010000000
VERSION     DAOOMA010000370.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 2744)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2744)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2744
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45302_length_2744_cov_3.944217"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..403
                     /locus_tag="PLP95_09805"
     CDS             <1..403
                     /locus_tag="PLP95_09805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Asd/ArgC dimerization domain-containing protein"
                     /protein_id="HPB46134.1"
                     /translation="GSVVDDGSEETDEEQKLRNESRKILGLSDLLVSGTCVRVPVFTG
                     HSLSINAEFDRAISPERAREILSQAPGVVLDELPTPLKSAGIDPTIVGRIRRDPTTDN
                     GLALFLSNDNLRKGAALNTIQIAEEILRRRG"
     gene            complement(423..677)
                     /locus_tag="PLP95_09810"
     CDS             complement(423..677)
                     /locus_tag="PLP95_09810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012223451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG nucleotide pyrophosphohydrolase
                     domain-containing protein"
                     /protein_id="HPB46135.1"
                     /translation="MEIAAFQQLMDDLYGQADRERGIPATVAWLCEELGELAQAVRKG
                     TTEEQLHELGDVFAWLASLANQIGLDLETAARRYVDNPPL"
     gene            complement(706..1185)
                     /locus_tag="PLP95_09815"
     CDS             complement(706..1185)
                     /locus_tag="PLP95_09815"
                     /inference="COORDINATES: protein motif:HMM:NF012415.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HPB46136.1"
                     /translation="MDRLNTSKDGWGRSARRTASIITALFLLVGFASGCESTDAERNE
                     VVSLVNQTRAASGLGQLAGNATLDIKADNWAQKLRNECRLSHSRLADGAPGEWRKLGE
                     NVGYGGTIAQVHTAYLNSPGHKANILDPSFTSMGAAAVWGMCDGQYRVFTVQVFMKS"
     gene            1240..2517
                     /locus_tag="PLP95_09820"
     CDS             1240..2517
                     /locus_tag="PLP95_09820"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HPB46137.1"
                     /translation="MSVLDELPSPNPQRLAVRVAPRALRHIRAGHPWVFDHSIESTSL
                     PGSPGDLAVVFDSKRNFAGIGLWDPNSPIRIRMLHAGKPRAIDDEFWAERIDAALALR
                     SGLIHSAATTGMRLVHGENDLMPGFTVDRYDTTLVVKIYSEAWLPHLNDVLLVLLDRC
                     GRAGLKIDRVVTRASRQVRSVMPVLDGAVVLGDEPSEPVVFLENGLKFYVDVVRGQKT
                     GHFLDQRDNRLMVRSFAGGRRVLDVFSSTGGFSVNAAAGGATSVLSVDLSSSALYLAK
                     KNMSLNAELSAVAECDHDIEIGDAFEELVRLAHGGRRFDLVIVDPPSFAQREADRPNA
                     LRAYRRLADLAMALVAPRGLYLQSSCSSRITMEELREAVLDAADATGRRVEIQSEHGH
                     AVDHPIGFEQGAYLKTIVVRFEPRAERGDAGRR"
BASE COUNT          449 a          861 c          914 g          520 t
ORIGIN      
        1 tggttcggtg gtcgacgacg gttccgagga aaccgacgag gaacagaagc tccgcaacga
       61 gagccgcaag atcctcggcc tttcggacct gctggtctcg gggacctgcg tgcgggttcc
      121 ggtgttcacc ggccacagcc tgagcatcaa cgccgagttc gaccgggcga tctcccccga
      181 gcgtgcccgt gagatcctct cccaggcccc gggggtggtg ctggacgagt tgccgacccc
      241 gctgaagtcg gccggcatcg acccgacgat cgtgggccgc atccgtcgcg atccgaccac
      301 agacaacggc ctcgcactgt tcctgtcgaa cgacaatctc cgcaagggag cggcactcaa
      361 cacgatccag atcgccgagg agatcttgcg ccgcaggggc tgaccgacgg cggttgccgt
      421 cgttacagcg gcgggttgtc gacgtagcga cgtgcggccg tttccagatc aagaccgatc
      481 tggttggcga gggaggccag ccaggcaaag acgtcgccga gttcgtgcag ttgctcctcg
      541 gtggtgccct tgcgcacggc ctgggcaagt tcaccgagtt cctcgcacag ccaggcgacc
      601 gtggcgggaa ttccccgctc acggtcggcc tggccgtaca ggtcgtccat cagttgctgg
      661 aacgctgcga tctccacagc gtcgaacgtt aggcgacagg tgtcgctacg acttcatgaa
      721 cacctggacg gtgaacacgc ggtactggcc gtcgcacatg ccccacaccg ccgccgcgcc
      781 catcgaggtg aacgacgggt cgaggatgtt ggccttgtgc ccggggctgt tgaggtatgc
      841 ggtatgcacc tgggcaatcg tgccgccgta gccgacgttt tcgccgagct ttcgccattc
      901 gcccggtgct ccgtcggcaa gtcgggaatg gctgaggcga cactcattgc gcagcttctg
      961 agcccagttg tcggccttga tgtcgagcgt ggcgttgccc gccagctgac cgagtccgct
     1021 ggcggccctg gtctggttga ccagggaaac cacctcgttt cgttctgcat cggttgattc
     1081 acaacccgat gcgaatccca cgaggagaaa cagtgcggtg atgatgcttg ccgtccggcg
     1141 tgcggaacgg ccccagccat ccttggaggt gttcaagcgg tccatggccc gtggtatcgg
     1201 tcacggtcgc cgcgagcctt gagtaagttt ccaccttcga tgagtgttct cgacgagttg
     1261 ccgtccccaa atccgcagcg tttggccgtt cgggtggctc cgcgagcgct tcgtcacatc
     1321 cgtgcgggtc acccgtgggt gttcgatcac agcatcgagt cgacctcgct gccgggaagt
     1381 ccgggcgacc tggcggtggt cttcgattcg aagcggaatt tcgcgggaat cgggctgtgg
     1441 gatccgaact ctccgatccg gatcaggatg ctgcacgccg gaaagcccag ggcgatcgac
     1501 gacgagttct gggccgagcg catcgacgcg gcgctggcgc tgcgtagtgg actgatccac
     1561 agcgcggcga ccaccggcat gcgcctcgtg catggcgaga acgatctgat gcccggtttc
     1621 accgtcgatc gctacgacac gacgttggtg gtgaagatct acagcgaggc gtggctaccg
     1681 cacctcaacg acgtgttgtt ggtgctgctc gaccgctgtg ggcgggcggg actcaagatc
     1741 gaccgtgtgg tcacccgtgc gagtcgccag gtccgatccg tgatgccggt cctcgacggg
     1801 gcggtggtgc tcggtgacga gccatcggag ccggtcgtgt tcctggagaa cgggttgaag
     1861 ttctacgtcg atgtggtccg cggccaaaag accgggcatt ttctggatca gcgggacaat
     1921 cgactcatgg tgaggtcctt cgcgggaggc cgtcgagtgc tcgatgtgtt cagctcgacc
     1981 ggcgggtttt ccgtcaacgc cgcggcgggc ggggcgacct cggtgctcag cgtggacctg
     2041 agttcgtcgg cgctgtacct cgcgaagaaa aacatgtcgc tcaacgccga gctttccgct
     2101 gtcgcggaat gcgaccatga catcgagatc ggcgacgcgt tcgaggaact ggttcggttg
     2161 gctcacggag gacgtcgttt cgatctcgtc atcgtcgatc cgccgtcgtt cgctcagcgc
     2221 gaggcggatc gacccaacgc gctgcgtgcc tatcgacgcc tcgccgatct cgccatggcc
     2281 ctggtggcac ccaggggcct gtacctgcag tcgtcgtgtt caagccggat cacgatggag
     2341 gagctccgcg aggcggtgct cgacgcagcg gacgccaccg gccggcgagt cgaaatccag
     2401 tccgaacacg gtcacgcggt cgaccatccc atcgggttcg agcagggcgc ctacctcaag
     2461 acgatcgtgg tgagattcga accgcgcgcg gagcgaggcg acgccggtcg gcgttgattc
     2521 attcaagttt tcggtcggtg aaggccgaag caattcgggt ccgatctcaa gctgggagtg
     2581 tcgaatccga tgccgtcgat ctcacgaggc gctacgcgcc gcctcgccgc cgccctttcc
     2641 gtgtccgccg tgttggtgac ctcggcctgc ctcgacaatg cgcagccgcc ggcgaccacc
     2701 gggctcgagg agatcgccgt gttcccgctg gcgatcccca tctc
//
LOCUS       DAOOMA010000371         2739 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_45418_length_2739_cov_3.480999, whole genome shotgun sequence.
ACCESSION   DAOOMA010000371 DAOOMA010000000
VERSION     DAOOMA010000371.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 2739)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2739)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2739
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45418_length_2739_cov_3.480999"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1412
                     /locus_tag="PLP95_09825"
     CDS             <1..1412
                     /locus_tag="PLP95_09825"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HPB46138.1"
                     /translation="VDRSSFDALRRGEGTIADGLRAARRGVAKARAVALNRSIPQYMT
                     DVVTSVPRPARTPASTPAVRSPRRIAAFSTYPIHPRRGGGQLRGWYLADALGRRDDTE
                     VTIVSLTTNPDLAGDHTLSDRVTEHCVLIDHGAARRETELRLVTGDVSITDIASGLMW
                     PGISGFTEVATRALDQACAAIAVQPYLIDAIDTLASGIATVYDSHNDEVELKATILPR
                     HTVGGRWLERWVDDLERRASEGSVLVTTTTEVDLRTLSARYRIDATTEVIPNGVDTGD
                     IEFIVGTARVARRQLLDPHLGLERNRPVALFVGSGHLPNIEAGRHIVSLARQVPGVDF
                     VLAGDHSTRLGLANLPPNVHAIGAVGDDLLELLLTSSTVALNPMGGGSGSNLKLFTYL
                     AAGLPVLSTTVGTRGIDPEAAGVMTTSVGSLAAGLDALLSEDGNERVLAGRNYVEANC
                     DWRVIADRFAALVVESTPS"
     gene            1409..>2739
                     /locus_tag="PLP95_09830"
     CDS             1409..>2739
                     /locus_tag="PLP95_09830"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HPB46139.1"
                     /translation="MTEDHRDQIPDQIPDQAFETGSDAMPRRTVFVEVTNTLAVNYIT
                     GFQRHTREMLGRLMQLDGPVRYIPVLWCSECETFRHLTTAELDAFAHFVPRPDPPRSR
                     LATLADPLPASLKRFGRRLIRTRAATSAREELARRRRLRNHPAAHDALRIDEWPDESW
                     FFDLEAAWHNVPHRSELLPWLRNRGVHTAVLVADVMPFLFHDWFDAGQIRLFTSFMQA
                     HLTYSEKFIAISECSRRDLLAVAEAEGVQRRLEVPIITMGANFKRADADLARPPEAPP
                     GRYILSVATVEPRKNHDLLIRAYDTLRDEIDDLSVVFVGKAGWMTDELQDRMRSHPDH
                     GGRFRWLDKVDDTLLNALFRHAYLAVQPAFYEGYGTPVIEALGNGVPTLSSSGGSLPE
                     AGGTYAEYFDPVDLDELVALIRRAYRDHAHYEAMRATLVDYRPPTWELGAAG"
BASE COUNT          441 a         1006 c          857 g          435 t
ORIGIN      
        1 acgtggatag gtcgtcgttc gacgcgctgc gccgcggtga gggcaccatc gccgacggac
       61 tgcgggcggc gcgacgcggc gtggccaagg cgcgggcggt tgcgctcaac cgttcgattc
      121 cgcagtacat gaccgacgtc gtcacctcgg ttccgcgacc ggcgagaacc ccggcgtcga
      181 cccctgcggt gcgatctcca cgccgtatcg cagcgttcag cacctacccc atccatcccc
      241 gccgcggcgg cgggcaactc cggggttggt acctggccga tgcgctggga cgccgcgacg
      301 acaccgaggt caccatcgtc agcctgacga ccaaccccga cctggccggc gaccacacgc
      361 tcagcgatcg ggtcaccgaa cactgcgtcc tcatcgacca cggcgcagcc cggcgcgaaa
      421 ccgagttgcg cctcgtcacc ggcgacgtgt cgatcaccga catcgcaagc ggcctgatgt
      481 ggccgggaat ctccgggttc accgaggtgg caacccgagc gctcgaccag gcctgtgccg
      541 ccatcgccgt gcagccctac ctcatcgacg cgatcgacac gctcgcgtcg gggatcgcga
      601 ccgtgtacga ctcccacaac gacgaggttg agctgaaggc cacgatcctt ccccgccaca
      661 ccgtcggtgg ccgttggctg gaacgttggg tcgacgacct cgaacgccga gcgagtgaag
      721 gctcggtgct ggtgacgacg accaccgagg tcgacctgcg cacgctgtcg gcccgctacc
      781 ggatcgatgc gaccaccgag gtcatcccca acggggtcga taccggcgac atcgaattca
      841 tcgtcggaac cgcccgcgtc gctcgccgac agctcctcga tccgcacctc ggcctcgagc
      901 ggaaccgacc ggtcgcactg ttcgtcggct cggggcacct gccgaacatc gaggccggac
      961 gccacatcgt ctcgctggcc cgccaggttc ccggcgtcga cttcgtcctt gccggcgatc
     1021 actcgacccg gctcggcctc gccaacctgc ctccgaacgt ccatgcgatc ggagccgtcg
     1081 gcgacgacct gctggagctg ttgctgacca gctccacggt cgcgctcaac ccgatggggg
     1141 gcggaagcgg atcgaacctc aagctcttca cctacctggc cgccgggctc ccggtgctgt
     1201 cgaccaccgt cggcacccgt ggcatcgacc ccgaggccgc tggggtgatg acgacatcgg
     1261 tcggttcact ggccgccggc ctcgacgcac tgttgagcga ggatggcaac gagcgggtcc
     1321 tcgccggccg caactacgtc gaggccaatt gcgactggcg ggtcatcgcc gatcggttcg
     1381 ctgccctcgt cgtagagtcg acgccatcat gaccgaggac caccgcgacc agatccccga
     1441 ccagatcccc gaccaggcct tcgaaaccgg gtccgacgcg atgcctcggc gcacggtttt
     1501 cgtcgaggtc accaataccc tggcggtcaa ctacatcacc gggtttcaac gacacacccg
     1561 cgagatgctc ggtcgtctca tgcaactcga cggtccggtg cggtatatcc cggtgctgtg
     1621 gtgttcggag tgcgagacct ttcgccacct caccaccgcc gagctcgacg cgttcgccca
     1681 cttcgtccct cgacccgatc ccccacgctc gcgccttgca acgctcgccg atccgctccc
     1741 tgcctcgctc aagcggttcg ggcggcgact cattcgcacg cgggcggcga catccgcgcg
     1801 cgaggaactc gcgcgccgac gtcgcctccg caaccacccg gccgcccacg acgcgctgcg
     1861 aatcgacgag tggcccgacg agtcgtggtt cttcgacctc gaggccgctt ggcacaacgt
     1921 gccgcaccgc agcgagttgt tgccgtggct gcgcaaccgc ggcgtgcaca ccgcggtgct
     1981 ggtggccgac gtcatgccgt tcttgttcca cgactggttc gacgccgggc agatccggct
     2041 gttcacctcg ttcatgcagg cgcacctgac ctactccgag aagttcatcg ccatctcgga
     2101 gtgcagccgg cgcgacctgc tcgccgtcgc cgaggccgag ggggtccagc gacggctcga
     2161 ggtgccgatc atcaccatgg gagccaactt caaacgcgcc gacgccgacc ttgcgcgccc
     2221 cccagaggca ccgcctggcc ggtacatcct gagcgtcgcc accgtcgagc cccgcaagaa
     2281 ccacgacctg ttgatccgcg cctacgacac ccttcgtgac gaaatcgatg acctgtcggt
     2341 ggtcttcgtc ggcaaggcgg gttggatgac cgacgagctg caggaccgca tgcgcagcca
     2401 cccggatcac ggcggacggt tccgctggct cgacaaagtc gacgacacgt tgctcaacgc
     2461 gctgtttcgc cacgcgtacc tggccgttca accggcgttc tatgagggct acggcactcc
     2521 ggtgatcgag gccctcggca acggggtgcc gacgttgagt tcgagcgggg gctcgttgcc
     2581 cgaggcgggc ggcacctacg ccgagtactt cgatccggtc gacctcgacg agttggtcgc
     2641 cttgatccgg cgcgcctacc gcgaccacgc ccactatgag gcgatgaggg cgactctggt
     2701 cgactaccgg ccaccgacct gggagctcgg agcggccgg
//
LOCUS       DAOOMA010000372         2730 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_45620_length_2730_cov_3.796262, whole genome shotgun sequence.
ACCESSION   DAOOMA010000372 DAOOMA010000000
VERSION     DAOOMA010000372.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 2730)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2730)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2730
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45620_length_2730_cov_3.796262"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..706
                     /locus_tag="PLP95_09835"
     CDS             <1..706
                     /locus_tag="PLP95_09835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB46140.1"
                     /translation="LDIMESLPKENQLLDDGHPDKFSDLATAWRDAGYVVAVPRFPVT
                     ADDAAVVDGSALTQRLADLSGQVGDVQFLLDWLLDETNAQDHRFAGRIDSDHVGYYGL
                     SYGSFTVWDAWFDAGSPDERVDALIQSDGGSNLTDEQLGSVPIPILVSHSDGDTTLPY
                     DNARRIFDALGSPKYLFTTHGHPHAMVGENTETNADEAYRQVTTAFWNRHLSGDEQAE
                     FPDSVVIEGVTSLEAG"
     gene            788..1072
                     /locus_tag="PLP95_09840"
     CDS             788..1072
                     /locus_tag="PLP95_09840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB46141.1"
                     /translation="MPANSKDAWGEVGSRLSALGLKLQLHAQEELSDEEIASKVGLER
                     LGAVAQEIADAISDAYSDEAVRADAKDVATAVRTAVEATARELRERATPE"
     gene            1154..2647
                     /locus_tag="PLP95_09845"
     CDS             1154..2647
                     /locus_tag="PLP95_09845"
                     /inference="COORDINATES: protein motif:HMM:NF025052.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HPB46142.1"
                     /translation="MWSSPLCRTSTPLAPEIAETHSAVVILLGDRAYKVKKPLDLGFL
                     DFTTVESRDAACRRELELNQRLAPDVYLGVAGIHGDDGEVCDSILVMRRLPADHRLST
                     CLDRGEDVSDALHRIAGDIAALHERSTRNPNLEAVGRREVVASRWRDGFAQLAAVPAG
                     IVEADRVTRIETLAIEYLDGREPLFESRIEHGQIVDGHGDLLADDIFVLDDGPRIIDC
                     LDFDDELRWGDVVADIAFLAMDLERLGLPDAALTFIEEYRTRSGRTWPTSLLHHYIAY
                     RAQVRAKVAAVRIAQEGDSASAARATRELQVLVEVCLHHLEAARVRLVLVGGTPGTGK
                     STVAAAIGEALGATVVRTDEVRREVRGEMPNRDGNPYSEAAVAATYNESLRRAAELLS
                     LGESVIIDATWSTESHRRLARATAAAASVPLSELRCVAPAEICDERITARLAIGTDPS
                     EATPDVAAAIRERFEPWPTSVAIATDTTMTQACQAALEAITADEVTN"
BASE COUNT          486 a          970 c          857 g          417 t
ORIGIN      
        1 cctcgacatc atggaatcgc tgcccaagga aaaccagttg ctcgatgacg gccaccccga
       61 caagttcagc gatctcgcca ccgcctggcg cgatgccgga tatgtcgtgg cggttccacg
      121 cttcccggtg accgccgacg atgccgccgt cgtcgacggg tccgcgctga cccaacgcct
      181 cgccgacctg tcgggccagg tgggtgacgt gcagttcctt ctcgactggc tcctcgatga
      241 gaccaacgcg caggaccacc gattcgccgg ccgcatcgac tccgaccacg tcggctacta
      301 cgggttgtcc tacgggtcgt tcaccgtctg ggatgcctgg ttcgacgcgg gttcgcccga
      361 tgaacgggtc gacgcgctga ttcagtctga cggcgggtcg aacctcaccg acgagcaact
      421 cgggtccgtg ccgatcccga tactggtctc ccattccgac ggcgacacca ccctcccgta
      481 tgacaacgcc cgccgcattt tcgacgccct cgggtcgccg aaatacctct tcaccaccca
      541 cggccacccc catgccatgg tcggagagaa caccgaaacc aacgccgatg aggcctaccg
      601 gcaggtcacc actgcgttct ggaaccgtca cctctccggc gacgagcagg ccgaattccc
      661 cgacagcgtc gtcatcgaag gggtcacctc actcgaggcc ggctgacccg gcggcgtcac
      721 caaccaggag ttctcgggga ccaacgaccc tgtttcaact cggcgagcgc acacaagagt
      781 gggccccatg cctgccaaca gcaaagacgc ttggggcgag gtcggcagtc gtctgtccgc
      841 cctcggcctg aaacttcaac tacacgccca ggaagaactc tccgacgagg agatcgcctc
      901 caaggtcggc ctggaacgtc tcggggccgt cgcccaggag atcgccgacg cgatcagcga
      961 cgcctactcc gacgaggccg tgcgcgcgga tgccaaggac gttgcgacag cggtgcgcac
     1021 cgccgtcgag gcgaccgcac gcgagcttcg cgagcgggcc acacccgagt agctcacggt
     1081 tccggcggga acccaacact tcaacacccc acggcgcccc acggcgcccc gatacaaccg
     1141 cgatgatgaa accatgtggt cttcgccgct ctgtcgcacc tccacaccgc tcgctcccga
     1201 gattgccgaa actcattcgg cggtcgtgat cctgctcggc gaccgcgcct acaaggtcaa
     1261 aaagcccctc gatctcggat ttctcgactt caccacggtg gagtcccgcg acgccgcgtg
     1321 tcgccgggaa ctcgaattga accaacgcct cgcgccggac gtgtatctcg gggtcgctgg
     1381 catccacggc gacgacggcg aggtctgcga ctcgatcctg gtgatgcgcc gcctgccagc
     1441 ggatcaccgc ctcagcacct gtctggatcg aggcgaggac gtgtccgacg cgctccaccg
     1501 catcgctggc gacatcgctg cgctccacga acgctcgacg cgaaacccca acctcgaagc
     1561 ggtcgggcga cgcgaggtcg tggccagccg ctggcgtgac ggcttcgcac aattggccgc
     1621 cgtgcccgcg ggaatcgtgg aggccgatcg cgtgacccgg atcgagacgc tcgcaatcga
     1681 atacctcgat gggcgcgaac cgctgttcga gtcgcggatc gaacacggac agatcgtcga
     1741 cggacacggc gacctcctcg cggatgacat ctttgtactc gatgacggcc cgcggatcat
     1801 cgactgcctc gacttcgacg acgaactgcg ttggggcgac gtcgtggccg acatcgcctt
     1861 tctggccatg gacctcgaac gcctcggcct ccccgatgcg gcactcacgt tcatcgagga
     1921 gtaccggacg cggtcggggc ggacatggcc gacatcgctg ttgcaccact acatcgccta
     1981 ccgggcgcag gttcgggcca aggttgcggc cgtgcggatt gcgcaggaag gtgacagcgc
     2041 ttccgcggca cgggcgactc gagaactgca ggtcctcgtc gaggtgtgcc ttcaccacct
     2101 cgaggcggcg cgggtgaggc tcgtgctcgt cggcgggaca ccgggaaccg gcaagtccac
     2161 ggtggccgcg gccatcggcg aggcactcgg ggcgacggtc gttcgcaccg atgaggtgcg
     2221 gcgtgaggtg cggggtgaga tgccgaaccg cgatggcaac ccgtacagcg aagcggccgt
     2281 cgccgccacc tacaacgagt cgctcaggcg ggccgccgag ctgttgtcgt tgggcgagtc
     2341 ggtgatcatc gacgccacgt ggtcgaccga atcgcaccgc cgactcgccc gcgcgacggc
     2401 ggccgcggca tcggttccac tcagcgaact gcgttgcgtt gcgccggccg agatctgcga
     2461 tgaacgcatc accgcacggt tggccatcgg aaccgacccc tcggaggcga cccccgacgt
     2521 ggccgcagcg attcgggagc gcttcgagcc gtggccgacc agcgtggcca tcgcgaccga
     2581 cacgaccatg acccaggcat gccaggccgc gctcgaggcg atcacggccg atgaggtcac
     2641 caactaggcc gcaggcctcc cccgcccggg aaccccgtcg atcactccga ggaacatgtg
     2701 tcgcagccgt cccgaccggt cgaggatcag
//
LOCUS       DAOOMA010000373         2727 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_45677_length_2727_cov_4.998129, whole genome shotgun sequence.
ACCESSION   DAOOMA010000373 DAOOMA010000000
VERSION     DAOOMA010000373.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 2727)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2727)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2727
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45677_length_2727_cov_4.998129"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1125
                     /locus_tag="PLP95_09850"
     CDS             <1..1125
                     /locus_tag="PLP95_09850"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF019334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HPB46143.1"
                     /translation="GIVHRDIKPANILVTRSHTPKLTDFGIAKGPEDADLTVVGTIIG
                     TAAYLAPEQVRSGPVDRRADLYALATVAYEALTGRAPFVGGDPAAVALARLHEAPRDL
                     TEQGVSPGLASVIMRNLEESPDRRHQSAADFLTALRNAARDPHRAVLNAQLNPAPTSI
                     ASETRPDRPTVPRQSVNTVPRRRSVIGTLVTTLLLVAPPVLVVALLATSASTTKITST
                     TTSPPSEIGPVEIASMSTFDPEGDGTEHDEETSNLGDGDLDSAWHTERYEARDFGTKS
                     GVGLIIDLGTSHELSELQLNGSTGWVGSVAVTETDPRIAGEIPGPSRPIAMTSSPIGI
                     PLDGTSGRFVTVWITELSAVEQGRHQVALSEVAVIGRSAA"
     gene            1122..1730
                     /locus_tag="PLP95_09855"
     CDS             1122..1730
                     /locus_tag="PLP95_09855"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HPB46144.1"
                     /translation="MNRKPEAASSVPHRDEDAELEDLARRSAQGDRRALEELLGALHA
                     RIATVCRRICGPDFDDAAQHAMINVARGIEGFDQRASVTTWAYRIATNASLDELRRRQ
                     RRGTSVDLDHVVIVDRSTPHSDSITDRMVIEAALDDLEPEFRSVIVMRETVGMDYAEI
                     AAALGVPIGTVRSRLSRAKGHLAAALRAGNQFDAADVQGDVL"
     gene            1727..2596
                     /locus_tag="PLP95_09860"
     CDS             1727..2596
                     /locus_tag="PLP95_09860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB46145.1"
                     /translation="MTVSPDSTPSDDRARADDLLNERVLAVLRSSEEFSDSDRDRHVA
                     LAMRALNKAEELGDSSGSQSIDLAEQRSQRRPGSTRSTRLLAAVAALLIGCLAAAILV
                     TNTGRSDDLADRADSNPQKEATADKSSGDGVADGTAHAPATAPPPVPDAAGGAESTEE
                     ASRALGQRQLGDFATLEGFAAAAMSTPTSADTDAQESGSLDDGFRDSTTANEAAPTGA
                     PQTTPSDAADATSGGPGSNPTNCDASSIAGVVDPALLSYGTVAGVGHLAWIDQSDGRL
                     ITLNLDDCTTVRH"
BASE COUNT          490 a          998 c          805 g          434 t
ORIGIN      
        1 ggcatcgtgc accgagatat caagccggcg aacatcctcg tgacccgatc ccacacgccc
       61 aaattgaccg acttcggaat cgccaaaggc cccgaagacg cagatctgac cgtggtcgga
      121 accatcatcg gcaccgcggc ctatctggct ccggaacagg tgcgatcggg tcccgtcgat
      181 cgtcgcgccg acctctacgc gcttgcgacc gtcgcctatg aggcgctcac cggtcgtgcg
      241 cccttcgtcg gtggcgaccc tgccgccgtc gcactcgccc gtttacatga agccccgcgc
      301 gacctcacag aacagggtgt gtcccccggc ctggcgtcgg tgatcatgcg caatctcgag
      361 gagtcacccg atcgccggca ccagtcggcc gccgactttc tcaccgccct gcgcaacgct
      421 gccagggacc cccaccgcgc ggtactcaac gcgcaactca acccggcacc gacgtcgatc
      481 gccagcgaaa cccgacccga taggccgacg gtcccgagac aatccgtcaa caccgtaccc
      541 cgccggcgat ccgtcatcgg caccctggtg accacgctgc tcctcgtcgc gccaccagtc
      601 ctcgtggtcg cgttactggc cacatcggcc tcgaccacca agatcacctc caccacgacc
      661 tccccgcctt cggaaatcgg acctgtcgaa atcgcctcga tgtcgacctt cgaccccgaa
      721 ggcgacggca ccgaacacga cgaggagact tccaacctcg gcgatggcga tcttgattcg
      781 gcctggcaca ccgagcgcta cgaagcacgc gacttcggta ccaagtccgg cgtcggtctc
      841 atcatcgacc tcggcaccag tcacgagctt tccgaactgc aactcaacgg gtccaccggc
      901 tgggtcggtt ccgtcgcggt gaccgaaacc gacccgcgaa tcgcggggga aattcccgga
      961 cccagccggc cgatagcgat gacttcctca ccgatcggca tcccgctcga cggcacttcg
     1021 ggccgcttcg tcacggtctg gatcacggag ttatccgccg tcgaacaggg ccgccatcag
     1081 gtcgcgctgt ccgaggtcgc cgtcatcgga aggtcggctg catgaaccga aagcccgagg
     1141 ccgcctcgag cgttccgcac cgcgacgaag acgcggaact ggaggatctc gcacgccgca
     1201 gcgctcaggg ggatcggcgg gcgctcgaag agttgctcgg cgcacttcac gcacggatcg
     1261 cgacggtatg tcgccgaatc tgtgggcccg acttcgacga cgccgcccaa cacgccatga
     1321 tcaacgtcgc ccgcggcatc gagggtttcg accaacgagc gtcggtcacc acgtgggcgt
     1381 atcgcattgc caccaacgct tcgctcgacg agctgcggcg ccggcaacgt cgcggtacgt
     1441 ccgtcgacct cgaccacgtc gtcatcgtcg accggtcgac gccgcattcc gattcgatca
     1501 ccgaccggat ggtgatcgag gccgccctcg acgatctcga accggagttc cgctccgtca
     1561 tcgtcatgcg tgaaaccgtc gggatggact acgccgagat cgccgccgcc ctcggtgttc
     1621 cgatcggtac ggtgcgctcg cgcctgtccc gtgccaaggg tcacctggcg gcagcgctgc
     1681 gggcggggaa ccaattcgac gccgccgacg tccaaggtga tgtcctatga ccgtttcacc
     1741 cgattccaca ccgagcgacg atcgggctcg cgccgacgac ctgctcaatg agcgggtcct
     1801 tgcggtgctg cgctcatcgg aggagttcag cgactccgac cgggatcgac atgttgcact
     1861 cgcgatgcgc gcgttgaaca aggccgagga actgggtgat tcgtccggtt cgcagtcgat
     1921 cgacctcgcc gaacaacgat cgcagcgacg gccgggttcg acgcgctcaa cgcgtctgct
     1981 cgcggcggtg gcagccctcc tcatcggctg tctcgctgcg gcgatcctcg tgacgaacac
     2041 cggtcgctcc gatgacctcg ccgaccgggc ggactccaac ccccaaaaag aggcgaccgc
     2101 cgacaagtca tccggggacg gcgtggccga cgggacagca cacgcaccag ctactgcccc
     2161 gccgccggtt cctgacgccg ctgggggtgc cgaatcgacc gaggaagcgt cccgggcgct
     2221 cggccaacgg caactcggcg acttcgcaac cctcgagggc ttcgccgctg cggccatgtc
     2281 gacgccaacc tcggccgaca ccgacgccca ggagtctggt tccctcgacg acgggttccg
     2341 cgactcgacc accgcgaacg aagccgcacc aaccggcgct ccccagacca ctccatccga
     2401 cgcagcggat gcaacgtcag gtggacctgg gtcgaatccg accaactgcg atgcctcctc
     2461 aatcgccggc gtcgtcgatc cagcgctgct gagttacgga accgttgccg gagtcggaca
     2521 tctcgcctgg atcgaccagt ccgacggtcg cctcatcacc ctcaatctgg acgattgcac
     2581 gaccgttcga cactgacggt tcggtggtgt tgtagcagcc ccatcggctg attgcgtaga
     2641 gtcactcgct atggccagcc ctcaggtgac cccaccgacg tcctcggagc ccgattggac
     2701 cgatcaggtc accggactcg tcgtcga
//
LOCUS       DAOOMA010000374         2718 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_45891_length_2718_cov_3.217799, whole genome shotgun sequence.
ACCESSION   DAOOMA010000374 DAOOMA010000000
VERSION     DAOOMA010000374.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 2718)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2718)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2718
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45891_length_2718_cov_3.217799"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            115..1029
                     /locus_tag="PLP95_09865"
     CDS             115..1029
                     /locus_tag="PLP95_09865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB46146.1"
                     /translation="MNGYQQEVAATFHRLRNEFRETCRAGSLFPGFGSGGIAALFVMA
                     SCRISHLDPRSVSADVHRCLAGRPDEFGTVTAWSVARNGRTLVGNCIKERNAKAHHTT
                     IRWALYVAARLFTMAVAAYVLEVIWLAATRDRAEIAPLVGPAAGNALAALGAAAAAKA
                     FRHHRPDNARIFFVMVGGAMAVSGSLGMAWSTVDPEIRTLGHLGLVIAGGFVACWANL
                     ASIDGHPTSLCRAFAGVGSVGFFVVFVVTAWEVLTSPIAGPIRAANTFGAFGWLILGT
                     GLATVAICGKSIGVGTGNCLNHDVTCAC"
     gene            1106..1894
                     /locus_tag="PLP95_09870"
     CDS             1106..1894
                     /locus_tag="PLP95_09870"
                     /inference="COORDINATES: protein motif:HMM:NF016041.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HPB46147.1"
                     /translation="MERSLLVLFVVGLVLVASEFVIRRHRNMDIDLHDTWVSAGVGSL
                     WFAAKAIGGYAGLIVVYRWISDQWAPWQLPLNNPLTWIGYWLVGDFFYYWVHRAEHRS
                     RWMWASHVVHHSSTDFGFTTAIRMPPTEILYKPLTGLWAPLLGFPPAMYAAMAAWGLI
                     TGQLQHTRIVGSLGVLDRWLATPSNHRVHHGSNERYLDKNFGGQTMIWDRIFGTYEPE
                     SEPVVYGVTEPLAGTRVRDVALGGYPALWQARRSRRLESAGAAV"
     gene            complement(1970..2476)
                     /locus_tag="PLP95_09875"
     CDS             complement(1970..2476)
                     /locus_tag="PLP95_09875"
                     /inference="COORDINATES: protein motif:HMM:NF012790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HPB46148.1"
                     /translation="MGGGIGRRATVESGDVLQVRPGDRLRVRVLPALLLTTALVAGGC
                     SDDQSDSAGSANVSAEATTGTDTPAAGVESATAALAEGRRVIDVRTAREFDEQHVEGA
                     DNLDIQGGSFDELIAELPTDGAYVVYCRSGNRSAVATKQMRAAGLDVVDGGAIADMES
                     AGWTITNG"
BASE COUNT          421 a          856 c          911 g          530 t
ORIGIN      
        1 agcgtcggtt cctattcaag gatgacatgg gggcgagtca tgcagtcgaa tacggcagta
       61 gcgattggtg ggggaatagt cctcgccggg gcagtgggtc tatccaggac cctgatgaac
      121 gggtaccaac aagaggtcgc ggcgacgttt catcgcctca ggaacgagtt tcgcgagaca
      181 tgtcgcgcag ggagtctgtt tccaggattc ggctcgggtg gaattgccgc actgttcgtg
      241 atggcatcat gtcgtatctc gcacctcgat ccgcgttcgg tcagtgccga cgttcatcgc
      301 tgccttgccg gccgaccgga tgagttcggc acggttaccg cttggtcggt tgctcgtaac
      361 ggtcgcaccc ttgtcgggaa ttgcatcaag gagcgaaacg cgaaggctca ccacacgacg
      421 attcgttggg ccctgtacgt ggccgcacgg ctcttcacca tggcggtcgc cgcatacgtg
      481 cttgaagtca tatggctggc tgctacgcgt gatcgagccg aaatagcgcc actcgttggc
      541 cctgctgcag ggaatgcttt ggcggcattg ggcgccgcgg cggcggcgaa ggccttccgc
      601 catcatcgcc ccgacaacgc tcgaatcttc ttcgtgatgg tcggcggagc aatggcggta
      661 tcggggtcct tggggatggc gtggtcgacc gtggaccccg aaatccggac gcttggtcac
      721 ctcggactcg tgatcgccgg tgggttcgtc gcgtgctggg cgaatctcgc atcgattgac
      781 ggccatccca cgtcgctgtg ccgggcgttc gctggcgttg ggtcggttgg gttcttcgtc
      841 gtattcgtcg taacggcctg ggaggttctc accagcccga tcgcagggcc gatccgtgcc
      901 gccaatacct tcggcgcctt cgggtggctc attcttggta ccgggctggc gaccgtcgca
      961 atctgcggca agagcatcgg tgtgggcaca ggcaattgcc tcaaccatga tgtgacctgc
     1021 gcctgttgag ctctgtagta cccccaggcg agtttggggg attgccccga tggtcccggc
     1081 gtccgtctcg ggcgatcgtc agtgcatgga acgttcactc ctcgtcttgt tcgtcgtcgg
     1141 cctggtgctc gtcgcctcgg aattcgtcat ccgccgtcat cgcaacatgg acatcgacct
     1201 gcacgacacg tgggtctcgg cgggcgtcgg ttcgttgtgg ttcgcggcga aggcgatcgg
     1261 cggatacgcg ggcctcatcg tggtgtaccg ctggatctcc gatcagtggg ccccctggca
     1321 actcccgctg aacaacccgc tgacgtggat cggctactgg ctggtcggcg acttcttcta
     1381 ctactgggtg catcgggcgg agcaccgcag ccggtggatg tgggcgagcc atgtcgtgca
     1441 tcactcatcc accgacttcg gcttcaccac ggcgattcgt atgccgccga cggagatcct
     1501 ctacaagccg ctcaccgggc tgtgggcccc gctcctcggc ttcccacctg cgatgtatgc
     1561 cgcgatggcg gcgtggggac tcatcaccgg gcaactccaa cacacccgca tcgtcggatc
     1621 gctcggtgtg ctggatcgat ggctggcgac cccgtcgaat caccgggtgc atcacggctc
     1681 gaacgagcgc tacctcgaca agaatttcgg tgggcagacg atgatttggg accgcatctt
     1741 cggcacctac gagccggaga gcgaacctgt tgtctacggc gtcaccgagc cgctcgccgg
     1801 cacccgggta cgcgacgttg cgctcggcgg ctacccggcg ctgtggcagg cgcgaaggag
     1861 ccgacgcctc gaaagcgccg gagccgcggt ctaggcgccc gggcagcgac agtgcgacgg
     1921 gccggcagcg acagcggcga cagggcgacc gtgcggctcg ggatgccggt cagccgttcg
     1981 tgatggtcca gccggccgat tccatgtcgg cgatcgctcc gccgtcgacg acgtcgagtc
     2041 cggcggcgcg catctgcttg gtggccaccg cggaacggtt gccggaccgg cagtagacga
     2101 cgtaggcgcc gtcggtcggc aactcggcga tgagttcgtc gaacgagccg ccctggatgt
     2161 cgagattgtc ggcgccctcg acgtgttgct catcgaactc acgggccgta cgcacgtcga
     2221 tcactcgtcg gccttcggcc agggcggccg tggcggattc gacgccggcc gccggagtgt
     2281 cggtgccggt tgtcgcctcg gccgagacgt tggcggaacc cgccgaatcg gattggtcat
     2341 cggaacaccc accggcgacg agggccgtcg tcagcagtag cgccgggagg acacggaccc
     2401 gcaacctgtc gccaggccgg acctgcaaga cgtcacctga ctcgacggtt gctcgtcgac
     2461 caattcctcc acccacggcg ctctcctcgt atcgatgtca cttgacgagt tcacgctacg
     2521 gcacggttgc gggcagaacg accggcgatg gtcccgatca cctgtgccat ccggctcgct
     2581 cgtttcgggt ccgcgtcggt gccggcctga aacggtgcgg gtcgcctcag cgattgagcg
     2641 cgaggaccaa ctcccgaatc tcatccggcg ccttcgaggg cgatccggcg tcgagcggag
     2701 gctgtgggtc gtattcga
//
LOCUS       DAOOMA010000375         2716 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_45943_length_2716_cov_2.166103, whole genome shotgun sequence.
ACCESSION   DAOOMA010000375 DAOOMA010000000
VERSION     DAOOMA010000375.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 2716)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2716)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2716
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45943_length_2716_cov_2.166103"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..293)
                     /locus_tag="PLP95_09880"
     CDS             complement(<1..293)
                     /locus_tag="PLP95_09880"
                     /inference="COORDINATES: protein motif:HMM:NF027659.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4968 domain-containing protein"
                     /protein_id="HPB46149.1"
                     /translation="MRRPPNAPPSTPPATPPATSPAALAEQMVVAGSVRVTVLTERLV
                     RIEYSADASFEDRATVSVVNRRFDPTPFEVTIDADTLNIDTGAVRVTCTNVTRP"
     gene            complement(300..1520)
                     /locus_tag="PLP95_09885"
     CDS             complement(300..1520)
                     /locus_tag="PLP95_09885"
                     /inference="COORDINATES: protein motif:HMM:NF012296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HPB46150.1"
                     /translation="MDAAATPTLRAPDSDLYYDPYDVAINADPYPVYERLREEAPIYH
                     NDRYGFWMLTRHDDVEKALVNWKAFSSARSDILDIIRAGYDLPGGVIMFEDPPAHTMH
                     RKLMSRIFTPRRMAELEDQVRRYCVACLDPLVGEPRFDIVEELARTLPMKVISMLVGI
                     PEQDQEAVRDKTDRNLRTRPGKPMEIREEEIASGSMFEDYIDWRADHPSDDLMTVLLN
                     AEFEDDNGETRRLTRQEVSTYTAVLAGAGNETTGRLIGWLIKVLADHPDQRRAVHEDR
                     SLLSRVIDETLRFEPTGHAIARYVTSDIDYYGTTVPAGSPILLSLAAANRDPRRYRDP
                     DTFDIGRNDITHLTFGYGLHYCLGASLARLEGRVALDEMLNRFPTWEVDTEAMRLFPT
                     STVRGWETMPVLIP"
     gene            complement(1523..2272)
                     /locus_tag="PLP95_09890"
     CDS             complement(1523..2272)
                     /locus_tag="PLP95_09890"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HPB46151.1"
                     /translation="MAELQGKVAVITGVGSGMGRAAAEVFVREGARVLAADVSGAQDD
                     TAAALGDAVVPLRVDVSNERDVEAMFSTALDAFGKVDAVLNVAGIAGAAPLADLSVEE
                     FDRFISVNLTGVMLGTKHGIRAMLPTGGGAILNWSSTGGLNGSRLPTAAYSASKAGVI
                     AITKAAAIEYGAEGIRANTICPGFVETAMSGGKGAFERFPQLVWSIALKRPGQPEEVG
                     ELASFLASDRATYITGAVIAIDGGSTAMLPS"
     gene            2326..>2716
                     /locus_tag="PLP95_09895"
     CDS             2326..>2716
                     /locus_tag="PLP95_09895"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HPB46152.1"
                     /translation="MANTRRARPATTEKRQRILDSTEEIILERGYAAVTSRSVAERVG
                     IQPPHLHYYFKTIDDLFVAVLHRRSHGAVERMSAALESSEPLRAWWALASDRRGTALL
                     VELLAAANHRAALRAEMAEMAKDIRRLQ"
BASE COUNT          427 a          850 c          959 g          480 t
ORIGIN      
        1 ggtctggtga cgttggtgca cgtcacccgc accgcgccgg tgtctatgtt cagcgtgtcg
       61 gcgtcgatcg tgacctcgaa cggggtcggg tcgaatcgac ggttgaccac cgacacggtg
      121 gcccggtcct cgaacgaagc atccgcgctg tactcgattc gaacgaggcg ctcggtcagg
      181 acggtgacgc gaacgctgcc agcgacgacc atctgctcgg cgagcgcggc gggggaggtg
      241 gccggcggag tggccggcgg agtgctcggt ggggcgttcg gtggacggcg catgtacggc
      301 tagggaatga ggaccggcat cgtttcccat cctcgcacgg tcgaggtggg aaacaggcgc
      361 atcgcttcgg tatcgacctc ccaggtgggg aagcggttca gcatctcgtc gagggcgacg
      421 cgaccttcga gccgagccag gctcgcgccg aggcaatagt gcagtccgta cccgaacgtg
      481 aggtgggtga tgtcattgcg gccgatgtcg aaggtgtcgg ggtcgcggta gcggcgcggg
      541 tctcgattgg cggccgcgag cgacagcaga atcgggcttc ccgccggcac cgtggtgccg
      601 tagtagtcga tgtcgctggt gacatagcgg gcgatcgcat ggccggtggg ttcgaagcgc
      661 agggtttcgt cgatcaccct cgacaggagc gaccggtcct cgtggaccgc gcgccgctgg
      721 tcggggtggt cggcgagcac cttgatgagc cagccgatga ggcgaccggt ggtctcgttg
      781 ccggccccgg cgagcaccgc ggtgtaggtc gacacctcct ggcgggtgag gcgtcgggtc
      841 tcgccgttgt cgtcctcgaa ctcggcgttc aacaacacgg tcatgaggtc gtcggagggg
      901 tggtcggcac gccagtcgat gtagtcctcg aacatcgacc cgctcgcgat ttcctcctcg
      961 cggatctcca tcggtttgcc ggggcgcgtg cgcaggttgc ggtcggtctt gtcgcgcacc
     1021 gcctcctggt cctgttcggg gattccgacc aacatgctga tgaccttcat cggcagggtc
     1081 cgagccaact cctcgacgat gtcaaagcgg ggctcgccga cgagcgggtc gaggcatgcg
     1141 acgcagtatc ggcgcacctg atcttcgagt tcagccatgc gtcgcggggt gaagatccgc
     1201 gacatgagct tgcgatgcat cgtgtgcgcc ggcgggtcct cgaacatgat gacgccaccg
     1261 ggaaggtcgt atccggcgcg gatgatgtcg aggatgtccg agcgtgcgct gctgaacgcc
     1321 ttccagttga ccagcgcctt ttcgacatcg tcatggcggg tgagcatcca gaacccgtac
     1381 cggtcgttgt ggtagatcgg cgcctcctcg cggagccgct cgtagaccgg atacgggtcg
     1441 gcgttgatcg cgacgtcgta ggggtcgtag tagagatcgg agtcgggtgc ccggagggtg
     1501 ggcgtcgcgg ccgcgtccat ggtcagctcg gcaacatggc ggtgctgcca ccgtcgatgg
     1561 cgatgaccgc gccggtgatg taggtcgccc ggtcggacgc gaggaacgac gcgagttcgc
     1621 cgacctcctc cggttgaccg ggacgcttga gcgcaatgga ccagacgagt tgcgggaacc
     1681 gttcgaaggc acccttgccg ccggacatgg cggtctcgac gaacccgggg cagatcgtgt
     1741 tggcgcggat cccttcggct ccgtactcga tggccgcggc cttcgtgatg gcgatgacgc
     1801 cggccttcga cgccgaatat gccgcggtgg ggaggcggga tccgttgagt ccgccggtcg
     1861 aggaccagtt gaggatcgct ccgccgccgg tcggcagcat cgctcggatg ccgtgtttgg
     1921 tgcccagcat caccccggtg aggttgaccg agatgaaccg gtcgaattcc tcgacgctca
     1981 gatccgcgag tggcgcggcc ccggcgattc cggcgacgtt cagcacagca tcgaccttgc
     2041 cgaacgcgtc gagcgccgtg ctgaacatgg cctcgacatc gcgctcgttt gaaacgtcga
     2101 cccggagtgg gacgaccgcg tcgccgagcg ctgcggcggt gtcgtcctgc gcgccgctga
     2161 cgtcggcggc cagcacccga gccccctctc gcacgaatac ttccgctgcc gctcggccca
     2221 tgccggaacc gacgccggtg atgactgcaa ccttgccctg gagttctgcc atgccgcaga
     2281 atgttcgagt tttaacgacc gttacaagta gttggggggc cggacatggc gaacacacgt
     2341 cgggcacgcc ccgccaccac cgagaagcgc cagcggatcc tcgactcgac cgaggagatc
     2401 atcctcgagc gcggttacgc cgcggtgacc tctcgcagcg tggcggagcg ggtcggaatt
     2461 cagcccccgc atctgcacta ctacttcaag accatcgacg atctcttcgt cgcggtcctg
     2521 caccgacgca gccacggtgc ggtcgagcgg atgagtgccg cactggaatc ctccgaaccc
     2581 ctgcgagcct ggtgggcgct ggcctcggat cgtcgcggca cggcgctgct cgtcgaactg
     2641 ctcgccgccg ccaatcaccg cgccgcattg agggccgaga tggcggagat ggccaaggac
     2701 atccgtcgac ttcaga
//
LOCUS       DAOOMA010000376         2711 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_46054_length_2711_cov_3.228539, whole genome shotgun sequence.
ACCESSION   DAOOMA010000376 DAOOMA010000000
VERSION     DAOOMA010000376.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 2711)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2711)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2711
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46054_length_2711_cov_3.228539"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(196..1104)
                     /locus_tag="PLP95_09900"
     CDS             complement(196..1104)
                     /locus_tag="PLP95_09900"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HPB46153.1"
                     /translation="MAHVTRGDVSLYWEEAGPDNGPAVLLIMGLGAQLIAWRPGFVER
                     LTDQGYRVIRFDNRDAGWSSSTSDRVLQIPDMVKLATGIDRGSADYLLSDMALDAAAV
                     LDAANVERAHVVGASMGGMIAQQLAIDLPERVASLTSIMSRPGSRLAGLPTVQVVRTV
                     MKPTPNDRAAMLEQELTRHETIGGPLFDRAATEVFLNEAYERTPERGGTPRQLAAILA
                     SPDRTPGLRLLQTPTLVIHGRQDRLVRPSGGRATAAAVPGSSLLMVDRMGHDLPQPLW
                     PLLTDAIGGHIRRAEHQRIDAGVAVG"
     gene            1209..2462
                     /locus_tag="PLP95_09905"
     CDS             1209..2462
                     /locus_tag="PLP95_09905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004180 - carboxypeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HPB46154.1"
                     /translation="MAFAETFDSIDALLEDLSSLYEDLHQHPELSFQEVRTAEILAKR
                     IEALGYDTTTGVGLTGVVGVLENGEGPTVLLRADIDALPVAEDTGLPYASTVVARDEE
                     GQEVGVAHACGHDMHATWMIGAATWLVEHRHLWSGKVLIVFQPAEEFGAGAKRMVDDG
                     LFERFGTPEVCLGQHLAPAPAGWVLLRAGPAMAASDAVKITLHGRGGHGSSPQMTVDP
                     AIMAASTIMKLQTVVSREVAPIEEAVVTVGTVRIGTKENVISDRAELKVNVRTFSDYV
                     RDKVLASIDRIAHGEAHSCGAPKDPEVEHLYHFPVLSNSEAETATVFSRFQSHFGFEN
                     TMEAPKATGSEDFGYFAESAGCPQVFWFTGGHDKERWIEAFNSGRLEEEVPFNHSPRF
                     APSQHPTIRTGIEALLVAAESWIGR"
     gene            complement(2532..2608)
                     /locus_tag="PLP95_09910"
     tRNA            complement(2532..2608)
                     /locus_tag="PLP95_09910"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2572..2574),aa:Pro,seq:ggg)
BASE COUNT          464 a          920 c          895 g          432 t
ORIGIN      
        1 ggtgtccggt ggccgaccgg gtctcgcctg tggagggttg agtctcgtgg tgtggaacgt
       61 cggcgtgcat ctttgccacc tccggtgccc tcattcggtg cagcaccatt gattcaccga
      121 tagtgacatg ttacgcgctc tgcgtgagat tgcggtctgg aacgcgtgag aacatcctca
      181 ggcgttcacc accgctcagc cgaccgcgac tccggcgtcg atacgttgat gctccgcccg
      241 ccggatgtga cctccgatcg cgtcggtcaa caacggccac aagggttggg gaaggtcgtg
      301 gcccatccgg tcgaccatca gcaacgacga cccgggcacc gccgcggccg tcgcccggcc
      361 cccgctcggg cgaacgaggc ggtcctggcg gccgtggatc accagggttg gcgtctgcaa
      421 caaccgcagc cctggcgtgc ggtcgggcga ggccagaatc gccgcgagct gcctcggagt
      481 gcccccacgt tcgggggttc gctcgtaggc ctcgttcaag aacacctccg tggcggcacg
      541 gtcgaacagc gggccaccga tcgtctcgtg gcgagtgagt tcctgttcga gcatcgcggc
      601 ccgatcgttc ggggtcggct tcatcaccgt gcgcaccacc tgcaccgtgg gcaggccggc
      661 aagtcgtgag cccggccgcg acatgatgga agtgagcgac gcgacccgtt cgggcagatc
      721 gatcgccaac tgctgggcga tcatgcctcc catcgaggcg ccgacgacat gggcccgctc
      781 gacgttggcg gcatcgagca ccgcggcggc gtcgagcgcc atgtcgctga gcaggtagtc
      841 ggccgaaccc cggtcgatcc cggtggcgag cttcaccatg tcggggatct gcagcacccg
      901 gtcgctcgtc gacgacgacc agccggcgtc gcggttatcg aagcggatga cccggtaccc
      961 ctgatcggtg aggcgttcga cgaaaccggg acgccaggcg atcaactgcg cgccgagccc
     1021 catgatcaac aacacggccg gcccgttgtc cggtccggcc tcctcccaat acagcgaaac
     1081 atcaccacgg gtcacgtgag ccatggatgc aacgctaccg agaggagagg tcctatggtg
     1141 ggcgaagcgc gcggacgacc cggttcgcgc tcgttcggcc gcatcgatgc ggcccgggga
     1201 ggaccacgat ggcattcgcc gaaactttcg actcgatcga cgcgttgctc gaggacctgt
     1261 cgtcgctgta cgaggacctc caccagcacc ccgagttgag tttccaggag gtgcgcaccg
     1321 ccgagatcct ggcgaagcgg atcgaagcgc tcggctacga cacgacgacc ggtgtcgggc
     1381 tcacaggcgt ggtcggggtg ctcgaaaacg gtgagggacc aaccgtgtta ttgcgcgccg
     1441 acatcgacgc gctgcccgtc gccgaggaca ccgggctgcc gtacgcgtcc accgtcgtcg
     1501 cccgagacga ggagggccag gaggtcggcg tcgctcacgc ctgtggccac gacatgcacg
     1561 cgacgtggat gatcggcgcg gcaacgtggc tcgtcgagca ccgacacctg tggtcgggca
     1621 aggtgctcat cgtgttccaa ccggccgagg aattcggggc cggggcgaag cggatggtcg
     1681 acgacggact cttcgaacgc ttcggcaccc ccgaggtctg cctgggccag cacctcgcac
     1741 cagcaccggc cggctgggtg ctgttgcgcg ccggaccggc gatggcggcc tcggacgcgg
     1801 tgaagatcac gcttcacggg cgcggcggac acggttcctc gccgcagatg accgtcgacc
     1861 cggccatcat ggcggcctcg accatcatga agctccaaac cgtcgtctcc cgcgaggtgg
     1921 cgccgatcga ggaggcggtc gtcacggtcg gcaccgttcg tatcggcacg aaggagaacg
     1981 tcatctccga ccgggccgaa ctcaaggtga acgtccggac gttctccgac tacgtgcgcg
     2041 acaaagtgct ggcctcgatc gaccggatcg cccacggcga agcccattcc tgcggagcgc
     2101 ccaaggaccc cgaggtcgaa cacctctacc acttcccggt gctgtcgaat tccgaggccg
     2161 agaccgcaac ggtgttctcg cgattccaga gccacttcgg gttcgagaac acgatggagg
     2221 cccccaaggc caccgggtct gaggacttcg gctacttcgc ggagtctgca ggatgcccac
     2281 aggtgttctg gttcaccggc ggccacgaca aggagcgctg gatcgaggcg ttcaacagcg
     2341 gacgcctcga agaggaggtt ccgttcaacc actccccccg gttcgctccg agccagcacc
     2401 ccacgatccg caccgggatc gaggcgctgc tcgtggcggc tgaaagctgg atcgggcgct
     2461 gacccgctgg ggcacccggt gctcgcggcg acctcgctga ttctccgccg ccgctgattc
     2521 tccgccgcaa ttggtcgggc tgacaggatt tgaacctgcg acctcttgac ccccagtcaa
     2581 gcgcgctacc aacctgcgcc acagcccgta ttcctcgact cggcacccga taggcgccgt
     2641 gccggggagt aactgtagcc gacacggtca ccggcgacga acctgcgacg aacctgcgac
     2701 tacttgaccc c
//
LOCUS       DAOOMA010000377         2704 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_46216_length_2704_cov_3.971310, whole genome shotgun sequence.
ACCESSION   DAOOMA010000377 DAOOMA010000000
VERSION     DAOOMA010000377.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 2704)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2704)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2704
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46216_length_2704_cov_3.971310"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..967
                     /locus_tag="PLP95_09915"
     CDS             <1..967
                     /locus_tag="PLP95_09915"
                     /inference="COORDINATES: protein motif:HMM:NF012926.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycoside hydrolase family 15 protein"
                     /protein_id="HPB46155.1"
                     /translation="MSCASLVRLGRFEPGLALLDWLVERVEHATRPDLLRPLYRVTGD
                     DPVPEATISEVAGYAGSRPVRIGNAAENQVQLDALGMVADLVHLIFTSGGGLRARHWA
                     LMEQLADVVVEHWREPDHGIWEVRLARQHNVVSKVMCWVVLDRAISLADATGRDVPER
                     WVAERDAIHGEVHSLGWSDRHETFTAQYGGESVDAGLLMMGLVGFIDPTEPRFASTIR
                     RIERELCEGIAVYRYQSDDGLSGVEGGMLICTGWLVQALARNGRVSDAADWFDRLVKP
                     VGPTGLLSEQVDPATERGLGNVPQAYSHLAVIDSAIALDECGYRR"
     gene            complement(995..2248)
                     /locus_tag="PLP95_09920"
     CDS             complement(995..2248)
                     /locus_tag="PLP95_09920"
                     /inference="COORDINATES: protein motif:HMM:NF017591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF839 domain-containing protein"
                     /protein_id="HPB46156.1"
                     /translation="MTEDNVDTHDQHTSDQHTSGPSTFGRRRLFRWAGAAGMLAATAP
                     LPVVTACSPQTIPGSQGSDANGLRLPPGFTSRIIAAGGQPVAGTTHVYGIFPDGGATF
                     VDRETPGGWYYVANHEVPFGGGGVTSFRFAPDGTIVDAYPICSWTSLNCAGGATPWGT
                     WLTCEEFDGGGVWECDPTRPRSARRHAAMGLFAHEAAAVAIDDRVYLTEDRPDGGFYR
                     FTPDRTGDLSSGLLEIACGPTHASEVGTVTWRRVPHVLADPVPCRRQVPDALHFDGGE
                     GIATSNDSVWFTTKGDNRIWHYSIATDAIDVRLDADPTMLGGVDNLWVTDGRLGDILL
                     VAEDGDDLQIVAVRSDNSMFEVVRVIGHPGSELAGPALSPDRTRLYFSSQRGPAPNDL
                     GPGAPGPITGVTYEVTGPFADLFNA"
BASE COUNT          404 a          816 c         1008 g          476 t
ORIGIN      
        1 gatgtcgtgt gccagcctcg tgcggctcgg acggttcgag ccgggtttgg cgctgctcga
       61 ttggctcgtc gagcgggtcg aacacgcgac gaggcccgac ctgttgcgtc cgctgtatcg
      121 ggtcaccggc gacgacccgg tgcccgaggc cacgatttcc gaggtggccg gatacgcggg
      181 aagtcggccg gtgcgaatcg gcaacgctgc agagaaccag gtgcaactcg acgcgctcgg
      241 catggtcgcc gacctcgtcc atctgatctt caccagtgga ggtgggcttc gggcgcgtca
      301 ttgggcgctg atggagcagc tcgcggatgt ggtggtggag cattggcggg agcccgatca
      361 cggcatctgg gaagtgcgcc ttgcccggca gcacaacgtg gtgtcgaagg tgatgtgttg
      421 ggtggtgctc gaccgcgcca tttcgctcgc cgatgcgacc gggcgagacg ttcccgaacg
      481 gtgggtggcc gaacgcgacg cgatccacgg cgaggtccac tctctcggat ggagcgatcg
      541 tcatgagacg ttcacggcgc aatacggcgg ggagtccgtc gatgccggcc tgctgatgat
      601 gggtctggtc ggtttcatcg atccgaccga gcctcggttc gcgtcgacga tccgtcggat
      661 cgaacgagaa ctctgcgagg gcattgcggt gtaccgctac cagagcgacg acgggctgtc
      721 gggggtggag ggaggcatgt tgatctgcac ggggtggctg gtgcaggcgt tggcccgcaa
      781 cggtcgcgtc tccgacgccg ccgactggtt cgaccgcctc gtgaaaccgg tgggccccac
      841 ggggctcctg agcgagcagg tcgacccggc caccgagcga ggcctcggca acgttcccca
      901 ggcgtactcg cacctcgcag tcatcgattc agccatagcg ctcgacgagt gtgggtatcg
      961 acgctgaacc cctcaaggct caggcgttga aggctcaggc gttgaagagg tcagcgaacg
     1021 ggccggtcac ctcgtaggtc acaccggtga tcgggcccgg ggcgccaggg ccgaggtcgt
     1081 tcggggcggg tccgcgctgg ctgctgaagt agagccgcgt ccggtcgggg ctcagcgccg
     1141 ggccggcgag ctcgctgccg ggatgtccga tgacgcggac tacctcgaac atggagttgt
     1201 ccgaacggac ggcgacgatc tgcaggtcgt cgccgtcttc ggccaccaac aagatgtcgc
     1261 cgaggcgtcc gtcggtgacc cagaggttgt cgactccgcc gagcatggtc gggtcggcgt
     1321 cgagtcgcac atcgatggcg tcggtggcga tcgagtagtg ccagatgcgg ttgtcgcctt
     1381 tggtggtgaa ccacaccgag tcgttcgagg tggcgattcc ctcaccgccg tcgaagtgaa
     1441 gtgcgtcggg gacctgcctt cggcagggaa cgggatcggc gagaacatgt ggcacccgcc
     1501 gccaggtcac ggtcccgacc tcggaagcat gtgtcgggcc gcacgcgatc tcgaggaggc
     1561 cggatgacag atctcccgtc cggtccgggg tgaatcggta gaacccgccg tcgggtcggt
     1621 cctcggtgag atacacccga tcgtcgatcg ccacggcggc ggcctcgtgg gcgaacaacc
     1681 ccatggccgc gtgtcgtcgt gcgcttcgtg gccgagtcgg gtcgcattcc cagacgccgc
     1741 cgccgtcgaa ctcctcacat gtcagccacg tgccccacgg ggtggcgccg ccggcgcaat
     1801 tgagcgaggt ccacgaacag atcgggtagg cgtcgacgat cgtgccgtcg ggagcgaaac
     1861 gaaaggaggt gacgccgccg ccaccgaagg ggacctcgtg gttggcgacg taataccacc
     1921 caccgggggt ttcgcggtcg acaaaggttg cgccgccgtc ggggaagatg ccgtagacgt
     1981 gggtggtgcc ggcgaccggt tggccgcccg ctgcgatgat cctgctggtg aagcccgggg
     2041 gaaggcgcag cccgttggca tcggatccct gggaccccgg gattgtttga ggggaacaag
     2101 cggtgactac ggggagcggg gccgtcgccg cgagcatgcc cgctgcaccg gcccaacgaa
     2161 acagccggcg gcgaccgaac gtggacgggc cgctggtgtg ctggtcgctg gtgtgctggt
     2221 cgtgcgtatc gacgttgtct tcggtcattg catggcgtcc ttttcccgag gtctccccaa
     2281 acccgatccc ccgaccggcg aacggtgcga gcggtgctcg gcgcaggata gagcctttct
     2341 gtcgcgatcg tcgctgttgt cgctgccgag ccccgcgacc agcgaccagc gacctgggta
     2401 gccccctgcg acgccgacgg cacaaacacg ggcgcgccgg tggccacgcc tgggcgtggt
     2461 gaagggcggg gtctgattgc gcgtgacctg ggtggggtga gtcctagtgg ggtgagtcct
     2521 agtgggcagg tccgggcttg tcctgaggag tctcagccat cggttcattg gcgtatcggt
     2581 ggttggggtg gtttggctgg ggtgagtgag agttgcccgg cggtggctcg cctggccctc
     2641 gtcgctctac cggttcgtcc agtcctgccc gagtttcggc gggtgcgggc gtcgtggggt
     2701 cggt
//
LOCUS       DAOOMA010000378         2701 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_46306_length_2701_cov_3.973545, whole genome shotgun sequence.
ACCESSION   DAOOMA010000378 DAOOMA010000000
VERSION     DAOOMA010000378.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 2701)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2701)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2701
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46306_length_2701_cov_3.973545"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..455
                     /locus_tag="PLP95_09925"
     CDS             <1..455
                     /locus_tag="PLP95_09925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB46157.1"
                     /translation="TVPLTPVLAVVPMAVVPMAVGADAAPAACPSQPDDASPVADPSP
                     VAMLAAALSVPAASAWAATRFGGGGMSQDSMRLSARQVLQIPLPVDHDLWRQAARASL
                     DPPADGDATGWRELGRLLNQSWRIDDDDLVDWWLARLPQMSRRSRRLR"
     regulatory      461..607
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01725"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="SAM-I-IV-variant riboswitch; SAM riboswitches
                     modulate the expression of downstream genes in response to
                     levels of S-adenosylmethionine; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF01725"
     gene            663..1874
                     /locus_tag="PLP95_09930"
     CDS             663..1874
                     /locus_tag="PLP95_09930"
                     /EC_number="2.3.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-acetyltransferase"
                     /protein_id="HPB46158.1"
                     /translation="MSQPSHPNQLEPVTGAWHPGLPAGRRQFATVSSGRPFPLEGGGH
                     LPEVVVAYETWGELDESASNAVLICHALTGDSHAAGRAAQGHPAEGWWDGLIGPGRAI
                     DTNRYFVVCSNVLGGCQGITGPASIDPRTGRPYGAAFPVVTIRDMVRAQALLATALGV
                     QRWASVIGGSMGGMQVLEWATMFPDRVGSWVSIASTIQASAQQIAWSMVGRRAIESDP
                     RFRGGDYYDAEPGDGPHRGLRLARQIAQIHYRSEEAFAEKFGRVELDRLDGPDGFTLD
                     SRFGVEGYLDYHGDKLLRRFDANSYLRLNKAMDLHDLARKRGSVEAALARVKAPSLVM
                     SISSDMLYPPYQQERIRDILIAANKRCGFYEIRSIDGHDGFLIELHQIAPLLEGFLDA
                     AWHDEQLGALR"
     gene            1871..>2701
                     /locus_tag="PLP95_09935"
     CDS             1871..>2701
                     /locus_tag="PLP95_09935"
                     /inference="COORDINATES: protein motif:HMM:NF013237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aspartate aminotransferase family
                     protein"
                     /protein_id="HPB46159.1"
                     /translation="MTDETTPRSDTVAIRAGREANGNALAPVLWASSAFVTPDAKTAQ
                     RFAQSTRAEQFYSRHGNPTINAFESAIAELEGAEAARSFASGMGAISAILLGLLSTGD
                     HVVTQRQLYGGTQMFFTAVCPRFGIDVTFVDAADPDAWDAAIIAGKTMMCFAESPANP
                     RLALVDLERFGAIGGPITVVDSTFATPIGVRPLDYGVNLVVHSATKSIGGHNDATIGV
                     VAGERELIDWLWGFAVLHGANASPFDAMNALRGLRTLPIRHQRQSATAQAIAEFLEAH
                     P"
BASE COUNT          437 a          980 c          869 g          415 t
ORIGIN      
        1 acacggttcc gctcaccccg gtgctcgccg tggtcccgat ggcggtggtc ccgatggcgg
       61 tcggcgcgga tgccgcaccg gcggcctgcc cgtcgcagcc ggacgacgcg tcgccggtgg
      121 ctgacccgtc gccggtggcc atgttggccg ccgcgctcag cgtgcccgca gcgtcggcct
      181 gggcggcgac gcgtttcggc gggggtggaa tgtcccagga ttccatgcgg ctctcggcca
      241 gacaggtgct tcagatcccc ctgcccgtcg accacgatct gtggcgacag gcggcgcggg
      301 cctcgctcga cccgccggcg gacggcgacg ccacgggctg gcgtgaactc ggtcgccttc
      361 tcaaccagtc ctggcgcatc gacgacgacg acctcgtcga ctggtggctg gcgcgcctgc
      421 cgcaaatgtc tcgaaggtcg cgccgcctcc ggtagcatgg ccgagtcatc tagagcggcc
      481 ccgaggggtc cggcaacctg ggacggacac ccaaggtgcc tctttcgacc agtgtcgtcg
      541 gcccacccgg accggcgtcg ccgaccgaaa ggatgcggcc ggttcgccgg caacgaattg
      601 tccgagtggc ccacctcggg cgactcgacg agtcagcggg tgtccgcctc cgatcgagcg
      661 tcatgagcca accgagccat cccaaccaac tcgaacccgt caccggtgcg tggcatcccg
      721 gcctgcccgc gggccgtcgc cagttcgcga cggtttcgag cggtcggccg ttccccctcg
      781 aaggcggcgg gcacctcccc gaggtcgtgg tggcctacga aacctggggc gaactcgacg
      841 agtcggcctc caatgcggtg ctgatctgtc atgcgctcac gggcgactcc cacgcagcgg
      901 gccgcgcggc gcaggggcat ccggccgagg gatggtggga cggcctgatc ggccccggcc
      961 gcgccatcga caccaaccgc tacttcgtcg tgtgttccaa cgtgttgggc ggatgccagg
     1021 gcatcaccgg acccgccagc atcgaccccc gcaccggccg tccctacggc gccgcctttc
     1081 cggtcgtcac catccgcgac atggtccgtg cccaggcgtt gttggccacc gcgctcggag
     1141 tgcaacggtg ggcgagcgtg atcggcgggt cgatgggtgg aatgcaggtg ctcgaatggg
     1201 cgacgatgtt tcccgaccgc gtcggttcgt gggtgtcgat cgcctcgacg atccaggcca
     1261 gcgcacaaca gatcgcctgg tcgatggtcg gacgccgggc gatcgagtcc gacccgaggt
     1321 tccgtggcgg cgactattac gacgccgaac ctggtgacgg cccgcaccgc gggctgcgcc
     1381 tcgcccgcca gatcgcgcag atccactacc gatccgagga agccttcgca gagaagttcg
     1441 gacgggtcga actcgaccga ctcgacggcc ccgacgggtt caccctcgac agtcgttttg
     1501 gggtggaggg ctacctcgac taccacggcg acaagttgct gcgccgattc gacgccaaca
     1561 gctacctgcg cctgaacaag gcgatggacc tgcacgacct ggcccgcaag cgcggcagcg
     1621 tcgaggccgc gctcgcccgg gtcaaagcgc cgtcgctcgt catgagcatt tccagcgaca
     1681 tgctctatcc gccgtatcag caagaacgga tcagagacat cctgatcgca gcgaacaagc
     1741 gctgcgggtt ctacgagatt cgcagtatcg acggccacga cgggttcctg atcgaactgc
     1801 accagatcgc accgctgctc gaaggattcc tcgacgccgc gtggcacgac gaacagttgg
     1861 gagcactccg atgaccgacg aaacgacccc tcgaagcgac accgtggcga tccgcgccgg
     1921 ccgtgaggcc aacggcaacg ccctcgcccc ggtgctgtgg gccagctccg cattcgtcac
     1981 tcccgatgcc aaaaccgcgc agcgtttcgc tcagagcacc cgcgccgaac agttctacag
     2041 ccgccacgga aaccccacga tcaacgcctt cgaatcggcc atcgcagaac tcgaaggagc
     2101 cgaagcggcg cgctcgttcg cctcggggat gggggcgatc tcggcgatcc tgctcggcct
     2161 gctgtccacc ggcgaccacg tcgtcaccca acgacagctc tacggcggca cccagatgtt
     2221 tttcaccgcg gtgtgcccgc gctttggcat cgacgtcacc ttcgtcgacg cggccgaccc
     2281 cgacgcctgg gatgcggcca tcatcgccgg caagaccatg atgtgtttcg ccgagagccc
     2341 ggccaacccg cgcctggcgc tggtcgacct cgaacggttc ggcgccatcg gcggaccgat
     2401 caccgtcgtc gactcgacct tcgccacccc gatcggcgtg cgccccctcg actacggggt
     2461 gaacctcgtc gtgcactcgg cgaccaaatc catcggcgga cacaacgacg ccaccatcgg
     2521 ggtcgtggcc ggtgaacgcg aactcatcga ctggctttgg gggttcgccg tgctgcacgg
     2581 tgccaacgcc tcgccgttcg acgccatgaa cgccctgcgc ggactgcgga ccctgccgat
     2641 tcgccaccaa cgccaatcgg ccaccgccca agcgatcgcc gagttcctcg aagcgcaccc
     2701 c
//
LOCUS       DAOOMA010000379         2693 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_46487_length_2693_cov_3.933283, whole genome shotgun sequence.
ACCESSION   DAOOMA010000379 DAOOMA010000000
VERSION     DAOOMA010000379.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 2693)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2693)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2693
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46487_length_2693_cov_3.933283"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..583
                     /locus_tag="PLP95_09940"
     CDS             <1..583
                     /locus_tag="PLP95_09940"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020378318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HPB46160.1"
                     /translation="EEGSFVEGDPLMPRSPYSAAKAGSDLIAMAYHETYELPVVITRS
                     SNQFGPYQFPEKIIPFFVTTLLEGGKVPLYGDGLNVRDWLFVLDNCRGVETVLRKGTV
                     GEVYNIGAGNEKTNREITDIILAALGKDESSVNYVEDRKGHDRRYSIATDKVGELGWA
                     PQVTFEEAIELTVQWYVDNRAWWAPLRERVRNR"
     gene            586..1452
                     /gene="rfbD"
                     /locus_tag="PLP95_09945"
     CDS             586..1452
                     /gene="rfbD"
                     /locus_tag="PLP95_09945"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: protein motif:HMM:NF016228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008831 - dTDP-4-dehydrorhamnose reductase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HPB46161.1"
                     /translation="MRIFVTGSNGQLGTELMQRLGDSHHEVVGVDVDTCDITDRDQIL
                     SAITQFAPDAIIHGAAFTAVDRCEAEPDVAFRVNSLATRFIADAATKVGAHVVYVSTD
                     YVFDGTKGTPYVEWDIPNPRSVYGASKLGGELELDPAWTVARTSWVCGLHGNNMVKTV
                     LRVAAERDELTFVSDQYGHPTFAGDLASKLIELAVGRARGVHHVTNQGAVSWYEFVQD
                     ILEAGGHSRDKVRPIMTAEFEAARPADAPPMAERPAYSVLDNFALRNSGFGELRDYRE
                     ALAEVVSALQAR"
     gene            1532..2539
                     /locus_tag="PLP95_09950"
     CDS             1532..2539
                     /locus_tag="PLP95_09950"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HPB46162.1"
                     /translation="MPSTPDVTAPTYDEQYYASYGGVNYAESTEFAALFHHFADRVVA
                     TLNPTKTLDAGCAIGRFVEALRARGVEAYGFDVSDYTIGQADPSIGEYVWVGSLTEAL
                     PDTYDLITCIEVIEHLPAAEAPVAVANLCAATDRILLSSTPYEYEEPTHLNVQPPEYW
                     STLFAAEGFFRVVDFDATFISPWAVLYERRPAQWADRVRDYDRSFWRLRDENVRLRNA
                     LLEATSNGGSGDPDITRADVLEWRERAQEADALGRRVDELESQLREAQHVRLRAVDAS
                     VAAERKAGTALGRVRELESEIARLQSLEHRYNEVINSTTWQLTWRALTPYRELRHRSG
                     R"
BASE COUNT          468 a          858 c          885 g          482 t
ORIGIN      
        1 cgaggagggc agcttcgtcg agggggatcc gctgatgccg cgctcgccgt actcggcggc
       61 gaaggccggc tcggacctca tcgccatggc ctatcacgag acctacgaac tgccggtggt
      121 catcacccgg agctcgaatc agttcggccc gtatcagttt cccgagaaga tcatcccgtt
      181 cttcgtgacg acgctgcttg agggcggcaa ggttccgttg tacggcgacg gactcaacgt
      241 ccgcgactgg ttgtttgtgc tcgacaactg ccgcggggtc gagacggtgc tgcgcaaggg
      301 aaccgtcggc gaggtgtaca acatcggtgc cggcaacgag aagaccaacc gggagatcac
      361 cgacatcatc ctcgccgcgc tcggcaagga cgagagctcg gtgaactacg tcgaggaccg
      421 taaggggcac gatcgccggt actccatcgc caccgacaag gtcggtgagc tcggttgggc
      481 tccgcaggtc accttcgaag aggcgatcga actgacggtc cagtggtacg tcgacaaccg
      541 agcgtggtgg gcgccgctgc gcgagcgggt gaggaaccgg tagccatgcg catcttcgtc
      601 accggatcga acgggcaact cgggaccgag ttgatgcagc ggctgggcga cagccatcac
      661 gaagtggtcg gggtcgacgt cgacacctgc gacatcaccg atcgcgacca gatcctttcc
      721 gccatcaccc agttcgcccc cgatgcgatc atccacggtg cggcctttac cgcagtcgac
      781 cgctgcgagg ccgaacccga tgtggcgttt cgcgtcaact cgctggcgac acgcttcatc
      841 gccgatgcgg caacgaaggt cggggcgcac gtggtgtacg tgtcgaccga ctacgtcttc
      901 gatggcacca agggcacccc ttatgtcgaa tgggacatcc ccaacccgag gtcggtgtac
      961 ggcgcgtcca agctcggtgg tgagcttgaa ctcgatccgg catggaccgt ggcccggacc
     1021 tcgtgggtct gcggcctgca cggcaacaac atggtcaaga cggtgctgcg ggtggcggcc
     1081 gaacgcgacg agctcacctt cgtcagcgac cagtacggcc acccgacctt tgcgggagac
     1141 cttgcctcga agctcatcga gctggctgtc ggccgggccc gcggggtgca ccacgtcacc
     1201 aatcaaggcg ccgtgagttg gtacgagttc gtccaggaca tcctcgaagc cggtgggcac
     1261 agccgcgaca aggtccgccc gatcatgact gcggagtttg aggcggcacg cccagcggac
     1321 gccccgccga tggccgagcg cccggcgtat tcggtcctcg acaacttcgc tttgcgcaac
     1381 agtgggttcg gcgaactgcg ggactaccgc gaggcgctcg ccgaagtggt ttctgcgctg
     1441 caggcgcgct gacagcgacg cccgcgttcg cggttcgggt ctggtccgcc gtgtcggaga
     1501 acccgctgtg tcaacgtcta tgctcttgcg cgtgccaagt acacctgacg tgacggcccc
     1561 gacctatgac gagcagtatt acgcctcgta cggaggggtg aattacgccg agtccacgga
     1621 atttgcggcg ctgttccatc atttcgccga tcgggtcgtg gcgacgctga atccgaccaa
     1681 aaccctcgac gcgggctgtg cgatcggtcg gttcgtggag gcgctgcgcg cccgtggcgt
     1741 cgaggcgtac gggttcgacg tgagtgacta tacgatcggc caagcggacc cctcgatcgg
     1801 cgagtacgtg tgggtcggct ccctgaccga agcacttccc gacacctacg acctcatcac
     1861 atgtatcgag gtgatcgagc accttcccgc cgcggaggct ccggtggcgg ttgccaacct
     1921 ttgtgcggcg acggatcgga tcttgttgtc gtcgactccc tatgagtacg aggagccgac
     1981 gcatctcaac gtgcagccgc cggagtactg gtcgactctc ttcgccgccg aggggttctt
     2041 ccgcgtcgtc gatttcgacg cgacgttcat cagcccgtgg gcggtgctct atgagcgtcg
     2101 gcctgcgcag tgggcagacc gggtacgcga ctacgaccga tcgttctggc gccttcgcga
     2161 cgagaacgtc cgtcttcgca acgcgttgct cgaagcgacc tccaacggcg gatcaggtga
     2221 tccggacatt actcgcgctg acgtgctcga gtggcgtgag cgggcgcagg aggctgacgc
     2281 tcttggccgg cgggtggacg aactcgagtc gcagttgcgc gaggcacaac acgtgcggct
     2341 gcgcgccgtc gatgcgtcgg tggcagcgga acgtaaggct ggtactgcgc tcggccgagt
     2401 gcgggaactc gagagcgaga tcgctcgtct ccaatcgctg gagcaccgct acaacgaggt
     2461 catcaactcc accacgtggc agctgacctg gagggcgctc acgccctacc gggagctccg
     2521 tcaccggtcg ggtcggtgac cgcaatgacc ccgcgtgttt ccatcgtcac tcccatctac
     2581 aacacccctg agcccgtgtt ccgggcaatg gtcgaatcgg tgagatccca gagcttcgtc
     2641 gactgggaac tgtgcctggt cgacggcaag tcgccgtcgc ctcatgtccg ccc
//
LOCUS       DAOOMA010000380         2676 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_46897_length_2676_cov_4.055322, whole genome shotgun sequence.
ACCESSION   DAOOMA010000380 DAOOMA010000000
VERSION     DAOOMA010000380.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 2676)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2676)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2676
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46897_length_2676_cov_4.055322"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..953
                     /gene="coaBC"
                     /locus_tag="PLP95_09955"
     CDS             <1..953
                     /gene="coaBC"
                     /locus_tag="PLP95_09955"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015939 - pantothenate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HPB46163.1"
                     /translation="ATARALSAYANGYSDDLLTATLIATRAPVIVCPAMHTEMWEHPS
                     VQDNLATLRRRGVIVVDPEEGRLAGGDVGAGRLAAPEVIVAAIEAQFTERDLEGLSVL
                     VSAGGTREPIDPVRYLSNRSSGRQGHALAEAAAARGAAVTLVTTTELPVGAGIERVAV
                     ASAAELHDAIMSRSRDSDVVIMAAAVADFTPVSVSEHKIKKADGVPLIELRPTVDILA
                     ALGAAKAPGQTIVGFAAETFDLEANARGKLERKGADLIVANDVSAPGVGFEHETNSVS
                     IFSATGRQVDIALTSKSAVANAVLDEVLLERSASDSGRLD"
     gene            1097..2293
                     /gene="metK"
                     /locus_tag="PLP95_09960"
     CDS             1097..2293
                     /gene="metK"
                     /locus_tag="PLP95_09960"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012229390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HPB46164.1"
                     /translation="MTNWTFTSESVTEGHPDKIADQVSDAILDAILAADPTARVACET
                     LVTTGLCIVAGEISTSAYVDIAGIARRTITGIGYDRESFGYDGNTCGVMVALDEQSPD
                     IAQGVDASEEARSGELTEDELDQQGAGDQGMMFGYATRETDVLMPLPIHVAHRLSERL
                     AEVRKSGAVPYLRPDGKTQVTFDYDENNQPVRLKTVLISTQHNDGIDRDTEIRPDLIE
                     QVIRPVIPERFADDDYDIYINPTGKFVIGGPVGDCGLTGRKIIVDTYGGMARHGGGAF
                     SGKDPSKVDRSAAYAARWVAKNVVASGAADRCEVQVAYAIGMAHPVSVLVETFGTNQV
                     DPAKLTQTVTEMFDLRPAAIARDLDLRKPRYQKTAAYGHFGRTDDEFTWERTDRADDL
                     RSALGL"
     gene            2398..>2676
                     /locus_tag="PLP95_09965"
     CDS             2398..>2676
                     /locus_tag="PLP95_09965"
                     /inference="COORDINATES: protein motif:HMM:NF036997.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB46165.1"
                     /translation="MRVVCDVAAVERTFDYLVPNRLIERFGSLQVGMMLRVPFHGRQV
                     AAWVVEVDCTPPEGVELREATKISSLGPPAEVIALAGWASWRWAGRGQT"
BASE COUNT          455 a          939 c          858 g          424 t
ORIGIN      
        1 cggccacggc ccgtgcgctg tcggcctacg caaacggcta ctccgacgac ctgctcaccg
       61 caacgctgat cgccacccgg gcaccggtga tcgtgtgccc ggcgatgcac accgagatgt
      121 gggagcaccc atcggtgcaa gacaacctcg ccaccctgcg gcggcgcggg gtgatcgtgg
      181 tcgaccccga ggagggtcgc ctcgccggcg gggatgtcgg agcgggtcgc ctcgcggcgc
      241 ccgaggtcat cgtcgccgcg atcgaggccc agttcaccga acgcgacctc gaagggttgt
      301 cggtgctcgt gagcgccgga ggaacccgcg aaccgatcga tcccgtgcga tacctgagca
      361 atcgcagttc cgggcgccag ggccacgccc tcgccgaggc ggcggctgcc cgcggggcgg
      421 cggtcaccct ggtcaccacc accgagttgc cggtcggcgc cggcatcgaa cgcgtggcgg
      481 tggcgagcgc cgccgaactg cacgacgcga tcatgtcgcg ttcacgcgac agcgacgtcg
      541 tcatcatggc cgccgcggtg gccgatttca cgccggtcag cgtgtcggag cacaagatca
      601 aaaaggccga cggcgtaccg ctcatcgaat tgcgtcccac cgtcgacatt ctggccgccc
      661 tcggagcggc caaggcaccg ggtcagacca tcgtcggatt cgcggccgag accttcgacc
      721 tcgaggccaa tgcccgcggc aagctcgaac gcaaaggcgc cgacctgatc gtcgcgaacg
      781 atgtgtcggc ccccggggtc gggttcgaac acgagacgaa ttcggtgtcg atcttttctg
      841 cgaccggccg ccaggtcgat atcgctctca cctccaagtc ggcggtggcc aacgccgtcc
      901 tcgacgaggt acttctggaa cgttcggcct ccgacagtgg ccgtttggac tgatccgcgg
      961 ctagacacga ctcacatcgg atgagccgaa ccgacccgcc gatccagccg atctgcccgg
     1021 cggggacgtt tcgatcacca cctaccgatt ccctttctca caaccatttc ccccgagtac
     1081 acgaggcacc atcaccatga ccaactggac gtttacgtcg gaatccgtga ccgaaggtca
     1141 tcccgacaag atcgccgacc aggtatcgga cgccatcctc gacgccatcc tcgccgccga
     1201 tccgaccgct cgggtcgcat gtgaaaccct cgtcaccacc gggctgtgca tcgttgccgg
     1261 tgagatctcc acctcggcct atgtcgatat cgccggcatc gcccgccgca cgatcaccgg
     1321 catcggctac gaccgcgagt cgttcggcta cgacggcaac acctgcggcg tcatggtcgc
     1381 cctcgacgag cagtctcccg acatcgccca gggtgtcgac gcatccgagg aggcccgctc
     1441 gggcgagctc accgaggacg aactcgacca gcagggtgcg ggcgatcagg gaatgatgtt
     1501 cggatacgcc acccgcgaaa ccgatgtgtt gatgcccctg ccgattcacg tcgcacatcg
     1561 tctttccgaa cgcctcgccg aggtccgcaa gtcgggcgcg gttccgtacc tgcgccccga
     1621 tggcaagacc caggtcacct tcgactacga cgagaacaac cagccggtgc gcctcaagac
     1681 cgtgctgatc tcgacccagc acaacgacgg catcgaccgc gataccgaaa tccgccccga
     1741 cctgatcgaa caggtcatcc gcccggtcat ccccgagcgg ttcgcggacg acgactacga
     1801 catctacatc aacccgaccg gcaagttcgt catcggaggc ccggtgggcg actgcggcct
     1861 gaccggccgc aagatcatcg tcgacaccta cggcggcatg gcgcgccacg gtggcggtgc
     1921 gttctcgggt aaagacccgt ccaaggtcga ccgttcggcg gcgtatgcgg ctcgatgggt
     1981 ggccaagaac gtggtggcct ccggcgctgc ggaccgttgc gaggtccagg tcgcgtacgc
     2041 catcggaatg gcacacccgg tgtcggtgct cgtcgaaacc ttcggcacga accaggtcga
     2101 cccggccaag ttgacccaga ccgtgaccga gatgttcgac cttcggccgg cggccatcgc
     2161 ccgcgatctc gacctgcgca agccgcgcta tcagaagacc gcggcatacg ggcacttcgg
     2221 acgcaccgac gacgagttca cctgggagcg caccgaccgc gccgacgatc tgcgttcggc
     2281 gcttgggctc tgagcccgcg gtcggcccgt tcgccgtcgt ccgggcgctt ggattcgacc
     2341 ccatcgttcg gttttgagtc cgtcccggag cgggcggacg atcgtgacgg acgcgtggtg
     2401 cgcgtcgtgt gcgacgtcgc cgcggtcgag cgaaccttcg actatctcgt cccgaaccga
     2461 ctgatcgagc gtttcggctc gttgcaggtc ggcatgatgc tgcgcgtgcc gttccacggg
     2521 cgtcaggtcg cggcatgggt cgtcgaggtc gactgcaccc cacccgaggg ggtcgagctg
     2581 cgcgaggcga cgaagatctc ctccctcggc ccgccggccg aagtcatcgc cctggccggg
     2641 tgggcgagtt ggcgttgggc ggggcggggg cagacc
//
LOCUS       DAOOMA010000381         2674 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_46952_length_2674_cov_3.609011, whole genome shotgun sequence.
ACCESSION   DAOOMA010000381 DAOOMA010000000
VERSION     DAOOMA010000381.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 2674)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2674)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2674
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46952_length_2674_cov_3.609011"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..271)
                     /locus_tag="PLP95_09970"
     CDS             complement(<1..271)
                     /locus_tag="PLP95_09970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB46166.1"
                     /translation="MTETSESPDDIAAARASRAVGSGAGGASAGGEPAGAEPSIGKKA
                     AGGFAWALVGFALMQVGSFATYSIATRVLGDEGIGVSATALTLVFW"
     gene            complement(296..1330)
                     /locus_tag="PLP95_09975"
     CDS             complement(296..1330)
                     /locus_tag="PLP95_09975"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012227115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HPB46167.1"
                     /translation="MKVSMQLPYAGGFDESVDQVVELEKAGLDIVYVAEAYSFDAVSA
                     MGYIAAKTSTVEIASGILPIYTRTPSLMAMTAAGVDYVSGGRCILGLGASGPQVIEGF
                     HGLTYDAPLGRTREMIDICRKVWAREEKLTYDGKYYQLPLPEGQGTGLGKPLKIINHP
                     VRARIPIQIAALGPKNIEMTAELAEGWIPIFYHPEKADDAFGEAIRKGTAKRDPELGQ
                     LDIVAGGLVCVGDDVKGVLDRLRPMVALYVGGMGARDKNFYNQLFQRYGYEAEAKEIQ
                     DLYLDGKKDEAAAAVPVEFLEATNLVGPEGYVKERIAAFKESGVTILSAIPMAENQPA
                     LIEQLKSWIS"
     gene            complement(1437..1664)
                     /locus_tag="PLP95_09980"
     CDS             complement(1437..1664)
                     /locus_tag="PLP95_09980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB46168.1"
                     /translation="MALVTDPVFNVNDRVVATVDLGAVPAGTPGRVILRNGISSFRYR
                     VFFDIGGPNGTDVGHVTGDVLARADRKGNRK"
     gene            complement(1648..2574)
                     /locus_tag="PLP95_09985"
     CDS             complement(1648..2574)
                     /locus_tag="PLP95_09985"
                     /inference="COORDINATES: protein motif:HMM:NF016228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar nucleotide-binding protein"
                     /protein_id="HPB46169.1"
                     /translation="MRVLITGGAGVLGSALVRVATERSDTGASADAGVSADTGANARH
                     QVEVTVRRRDSVTPPGAKRHIVELTQPMAFWSVVERRRPDVVIHTAYSRSDHVATVDI
                     TSEVATVCATFGVELIHVSTDALFDGDHAPYDESALPAPVTPYGRAKARCEEIVSENC
                     PDAAIVRTSLIVADDASDGTSAWLVEALRAGQRTTLFHDEFRCPIRDVDLAKLLWGLV
                     DLDAAERRGVWHLVGPERLSRVELGHRIARRMGLDGSLIDEASSDSMPGRRPKDLALG
                     AARMQRWLESGRSGDACWPAPIGSVEGHGSRN"
BASE COUNT          476 a          953 c          798 g          447 t
ORIGIN      
        1 tccaaaagac caaggtgagc gcggtggcgg agacgccgat cccctcgtcg ccgaggactc
       61 gagtggcaat gctgtaggtc gcgaacgacc cgacctgcat cagtgcgaag ccgacgagcg
      121 cccacgcgaa cccgccggca gccttcttgc cgatcgatgg ctcggcgccg gcgggctcgc
      181 cgccggccga cgcaccaccc gcaccagacc caactgcgcg cgaggccctc gccgcggcga
      241 tgtcgtcagg ggattcggag gtttcggtca caggcagcgg atcacaggca gcggatcagg
      301 agatccagct cttgagctgt tcgatcaacg ccggttgatt ctccgccatc ggaatggccg
      361 acaggatggt cacccccgac tccttgaacg cggcgatgcg ttccttcacg tatccctcgg
      421 gcccgaccag gttggtcgcc tcgaggaact ccaccggaac ggccgcggcg gcctcgtcct
      481 tcttgccgtc gagatacagg tcctggatct ccttggcctc cgcttcgtag ccgtaccgct
      541 gaaagagttg gttgtagaag ttcttgtcgc gagcccccat gccgcccaca tacagcgcca
      601 ccatcggccg gagacgatcg agcacccctt tgacgtcatc tccgacgcac acgagacccc
      661 cggccacaat gtcgagctgt ccgagctcag gatctcgctt ggcggtgccc ttgcggatcg
      721 cctcgccgaa cgcgtcgtca gccttttccg ggtggtagaa gatcggaatc caaccctctg
      781 cgagctcggc ggtcatctcg atgttcttcg gtcccagcgc cgcgatctga atcgggatac
      841 gagctcgaac cggatgattg atgatcttga gcggctttcc gagcccggtc ccttgcccct
      901 cgggcaacgg cagctggtaa tacttgccgt cgtaggtgag cttctcctcg cgggcccaca
      961 ccttgcgaca gatgtcgatc atctcacggg tacgcccgag gggagcgtcg taggtcaggc
     1021 cgtggaaccc ctcgatgacc tgcggacccg aggctccgag accgaggatg caacgtccac
     1081 ccgagacgta atccaccccg gcggcggtca tcgccatcag cgacggcgtg cgggtgtaga
     1141 tcggcaaaat tcccgatgca atctccaccg tcgaggtctt cgccgcgata tagcccatgg
     1201 ccgagaccgc atcgaagctg tacgcctcgg cgacgtagac gatgtcgagc cccgcctttt
     1261 ccagctcgac cacctgatcg accgactcat cgaaaccacc ggcgtacggc agttgcatcg
     1321 aaaccttcat gatgttgttt cccccctgtt gggttgtgaa cgtcgacccc ggtgtcacac
     1381 cagtcgtcga cctggcacca gcaccggcga gcaccggccc cccacgaact atttgcctat
     1441 ttgcggtttc ccttgcgatc ggctcgggcc aacacgtccc cggtcacgtg gccgacgtcg
     1501 gttccattcg ggccaccgat atcgaagaac acgcgatagc gaaacgacga gatgccgtta
     1561 cgaaggatca cccgccccgg ggtgccggcc ggaaccgctc cgaggtcgac ggtggcgacg
     1621 acgcgatcgt tgacgttgaa cacggggtca gttacgagag ccatgacctt ccacactacc
     1681 gatcggcgca ggccagcacg catccccgga ccgtcccgat tcgagccacc gctgcattcg
     1741 agccgcaccg agggccagat ccttcgggcg gcggcccggc atcgagtccg acgaggcctc
     1801 atcgatgagc gaaccgtcga gacccattcg acgggcgatt cgatggccga gttccacccg
     1861 gctgagacgc tccgggccga cgaggtgcca cacaccccga cgttcggcgg cgtccaggtc
     1921 gacgaggccc cacagcagct tcgcaaggtc gacgtcgcgg atcgggcacc ggaactcgtc
     1981 gtgaaacaag gtcgtgcgct gcccggcccg caacgcctca accaaccagg ccgaggttcc
     2041 gtcgctcgcg tcgtcggcga cgatcaacga cgtccgcacg atcgcggcgt cggggcagtt
     2101 ctcgctcacg atctcctcac accgcgcctt cgcccgccca taaggcgtga ccggtgccgg
     2161 gagcgccgac tcgtcgtagg gcgcatgatc accgtcgaag agggcgtcgg tggagacgtg
     2221 gatcaactcc acgccaaacg tcgcacacac cgtcgccacc tcggaggtga tgtccacggt
     2281 cgcgacgtga tcgctgcgcg aataggcggt gtggatgacc acatcggggc ggcgccgctc
     2341 gacgacgctc cagaacgcca tcggttgcgt gagttcgacg atatggcgtt tggccccggg
     2401 gggcgtgacg gagtctcggc gccgaacggt gacctccacc tggtggcgag cgttcgcccc
     2461 ggtatcggcg ctcaccccgg cgtcagcgct tgccccggta tcgctccgtt cggtagccac
     2521 acgcacgagc gccgatccaa gcacccctgc tccgccggtg atcagcaccc tcatcgcacc
     2581 ctcctcatcg caccctcctc atcgcaccct cctcatcgca ccctcctcac gagccccctc
     2641 acgaccccct cacgaacccg aacccgagcc ggca
//
LOCUS       DAOOMA010000382         2671 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_47010_length_2671_cov_3.798930, whole genome shotgun sequence.
ACCESSION   DAOOMA010000382 DAOOMA010000000
VERSION     DAOOMA010000382.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 2671)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2671)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2671
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47010_length_2671_cov_3.798930"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            96..596
                     /locus_tag="PLP95_09990"
     CDS             96..596
                     /locus_tag="PLP95_09990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020379083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MogA/MoaB family molybdenum cofactor
                     biosynthesis protein"
                     /protein_id="HPB46170.1"
                     /translation="MSAQDQTQNAGVRYEAKVLTVSDSVVLGNRPDTSGPAVVEYLEE
                     HGFTVVDSRVCADGSETVANQLSSMSFNFWGIIVTAGGTGFGPRDLTPEGTVRILDRR
                     AYGFSEAMRATDPKGRLSRGVSGTRGSTLIMNVAGSPSASRPMLDAVIDVVPYPLDLM
                     AGKTPD"
     gene            752..1807
                     /gene="ribD"
                     /locus_tag="PLP95_09995"
     CDS             752..1807
                     /gene="ribD"
                     /locus_tag="PLP95_09995"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012229402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HPB46171.1"
                     /translation="MSENFTAIDRECMARAIAAASQVRCATAPNPWVGAVVRGADGVI
                     YEGATMEPGKAHAEIVALAAAGTAAQGSTVYVTLEPCSHTGRTPPCVDALIEAGISRA
                     VIALEDPDERVNGRGIEALRAAGITVEVGLYADRVATQLAPYIKHRTTGRPYVVLKMA
                     ASVDGFTAAPDGSSQWITGPAARADGHRIRAESDAVIVGAGTVRRDNPSLTVRDYHPP
                     VLPEHGTVDPLRVILANSAVDPEAKVQPCREMRGELVDILDELGNDGILQVLVEGGAQ
                     VAGDFHRAGLVDKFVLYLAPALFGGGDARGLFTGLGAYSIDDVWRGRFDTVERVGGDL
                     RLELTPATAATDSQERR"
     gene            1810..2430
                     /locus_tag="PLP95_10000"
     CDS             1810..2430
                     /locus_tag="PLP95_10000"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012229403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HPB46172.1"
                     /translation="MFTGIVEELGTVVSREGSRLRIAAAAVLGGVQLGDSTVVNGVCL
                     TVVAWQDPTDAHPGWWEADVSPETYQRSALGTLEPGDRVNLERPVRLMDRLGGHLVQG
                     HVDAVGTVAAAVPDLRVTMPAELTRYVVEKGSITVDGVSLTVVDALDDGFTVAVIPHT
                     AEVTTLGQRGVGDLVNLEVDVTAKYIERLIAAHLPSAESGQRGTAS"
     gene            2427..>2671
                     /locus_tag="PLP95_10005"
     CDS             2427..>2671
                     /locus_tag="PLP95_10005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012229405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HPB46173.1"
                     /translation="MTRGATNPDSIFATIPEAVAAIGRGEIVVVVDDEDRENEGDLIM
                     AAEHATPEAITFFVRHTSGVICAPLTGDRLGELDIPL"
BASE COUNT          453 a          932 c          877 g          409 t
ORIGIN      
        1 gacagcgacg ggatggcgct gttcatctgg cggtatcgcc cgtagcggta gcacccgtgg
       61 cggtgagggt ggccggaggg cgtagtctct gggccatgag tgcgcaggac cagacccaga
      121 acgccggtgt tcgctacgag gcgaaggtgc tcaccgtttc ggacagcgtg gtgctcggca
      181 atcggcccga taccagcggg ccggccgtgg tcgagtacct cgaggaacac gggttcaccg
      241 tcgtggactc gcgggtgtgc gccgatggtt ccgagaccgt ggccaaccag ctgtcgtcga
      301 tgtcgttcaa cttctggggg atcatcgtca ccgccggcgg gacgggattc gggcctcggg
      361 atctcacccc cgaaggaacc gttcgcatcc tcgaccgtcg ggcctacggg ttctccgagg
      421 ccatgcgtgc caccgacccc aagggcaggt tgtcgcgagg ggtgtcgggt acgcggggca
      481 gtacgctcat catgaacgtc gccggctcgc cgagtgcgtc gcggccgatg ctcgatgccg
      541 tgatcgatgt cgtgccctat ccgctcgacc ttatggccgg taagaccccg gactgaaccg
      601 ggccgaaacg ccgcaccgca gaacgccgca ccgcaacaca gcgttccgcc cgcaccgttc
      661 cgccacatcc cgttccgcca catcccgttc cggtcgaaat cggaacgacc gctacaattt
      721 cgaccgaccg cctgttaccc tcacgaggac catgtcagag aacttcaccg cgatcgaccg
      781 cgaatgcatg gcgcgtgcga tcgccgccgc atcacaggtt cgatgtgcaa ccgcgcccaa
      841 tccgtgggtc ggcgccgtcg ttcgcggtgc ggacggggtg atctacgaag gggcgacgat
      901 ggagcccggc aaggcccacg ccgaaatcgt cgcgctcgcc gccgcaggaa ccgcggcaca
      961 gggttccacg gtctatgtca ccctcgagcc gtgcagccac accgggcgca ccccgccctg
     1021 tgtcgatgcg ctcatcgaag ccggaatctc ccgggcggtc atcgccctcg aggaccccga
     1081 cgaacgggtg aacggtcggg ggatcgaggc gctgcgtgcc gcgggcatca ccgtcgaagt
     1141 cgggctgtac gcagatcggg tcgccaccca actggcgccc tacatcaaac accgaaccac
     1201 cggccgcccc tatgtcgtgt tgaagatggc ggcgtcggtg gatggattca ccgcagcccc
     1261 cgacggttcg agccagtgga tcaccggccc cgcagcacgc gcggatggcc atcgcatccg
     1321 cgccgagagc gatgcggtca tcgtcggtgc cgggaccgtg cgacgcgaca atccctcgct
     1381 caccgtgcgc gactatcacc cgccggtgct gcccgaacac ggcacggtcg acccgcttcg
     1441 agtcatcctc gccaactcgg cggtcgaccc cgaggcaaag gtgcagccct gccgcgagat
     1501 gcgcggcgag ctcgtcgaca tcctcgacga gctcggaaac gacggcatct tgcaggtgct
     1561 cgtcgaaggc ggcgcccagg tcgccggcga tttccaccgc gccggcctcg tcgacaagtt
     1621 cgtgctctac ctggccccgg cgctgttcgg cggcggcgac gcccgagggc tgttcaccgg
     1681 cctcggcgcc tacagcatcg acgacgtctg gcggggtcga ttcgacacgg tcgaacgggt
     1741 cggcggcgac cttcgcctcg aactcacgcc cgccaccgct gcgacggatt cacaggaacg
     1801 gagatagagg tgttcaccgg catcgtcgag gaactcggaa ccgtcgtgtc ccgcgagggc
     1861 tcccggctgc gcatcgccgc ggccgcggtg ctcggcggag tgcaactggg cgactccacc
     1921 gtggtcaacg gggtgtgtct caccgtggtc gcctggcagg acccgaccga cgctcacccc
     1981 ggatggtggg aggccgacgt gtcgcccgag acctaccaac gcagtgcgct cggaacgctc
     2041 gaaccgggcg accgggtcaa cctcgaacgc ccggttcggc tcatggatcg cctcggcgga
     2101 cacctcgtgc agggtcacgt cgacgccgtc ggaaccgtcg ccgctgcggt gccggacctg
     2161 cgggtcacga tgcccgcgga actgacccgt tacgtcgtcg agaaggggtc gatcaccgtc
     2221 gacggcgtct cactcaccgt ggtcgatgcg ctcgacgacg ggttcaccgt ggcggtcatc
     2281 ccacacactg ccgaggtcac caccctcggg caacgagggg tgggcgacct ggtgaacctc
     2341 gaggtcgacg tgaccgccaa gtacatcgaa cgactcatcg ccgcccacct gccgagcgca
     2401 gagtcgggcc agagaggaac cgcatcatga ccagaggtgc aacaaacccc gattcgatct
     2461 tcgccacgat ccccgaggcg gtcgcagcca tcggacgcgg ggagatcgtc gtggtcgtcg
     2521 acgacgagga ccgtgagaac gaaggcgatc tcattatggc cgccgagcac gccacccccg
     2581 aggcgatcac gttcttcgtc cgccacacct ccggggtgat ctgtgctccg ctcaccggag
     2641 accggctcgg cgagctcgac atcccactca t
//
LOCUS       DAOOMA010000383         2664 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_47168_length_2664_cov_3.706018, whole genome shotgun sequence.
ACCESSION   DAOOMA010000383 DAOOMA010000000
VERSION     DAOOMA010000383.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 2664)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2664)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2664
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47168_length_2664_cov_3.706018"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1670)
                     /gene="ilvB"
                     /locus_tag="PLP95_10010"
     CDS             complement(<1..1670)
                     /gene="ilvB"
                     /locus_tag="PLP95_10010"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005948 - acetolactate synthase complex
                     [Evidence IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic-type acetolactate synthase large
                     subunit"
                     /protein_id="HPB46174.1"
                     /translation="MGEQQGEQPTRMTGAEALIKALELEGVEVIFGLPGGCILPVYDP
                     IIDSPIRHVLVRHEQGAGHMAEGYAHATGRPGVAMVTSGPAATNIVTPLCDAFMDSVP
                     MVVITGQVSVAAIGTDAFQECDTIGITRSVTKHNELVTSAQDIPLAVRQAFHLATTGH
                     PGPVLLDIPKDIIDPTHPNSMMEWHWPTDEEVAASLPGYKPTTKGHPRKIKEAAELIT
                     AAHRPVLYVGGGVLKARGAEALRELAELADIPVVTTLMARGALPDDHELNLGMPGMHG
                     NYTAVTAMQESDLLIALGSRFDDRVTGKLDGFAPDAKIIHIDIDPAELGKVRRPDVPI
                     VGDARLVAEELIRVLRNQGAPDNQTDRTPWRSTLSGWQEKYPLTYEQSAPGGLLKPQF
                     VLEQIRDLSPRDTIVASGVGQHQMWTAQYWNFQYPYTWINSGGLGTMGFSVPAAIGAK
                     VGMPDRTVWAIDGDGCFQMTAQEMVTAATERIPVKIAILNNAYLGMVRQWQEMFYEER
                     YSEVYLSPDLPDYVKWAEAMGCVAMRVESPEEVAPAIEKANSINDRPVV"
     gene            2074..>2664
                     /locus_tag="PLP95_10015"
     CDS             2074..>2664
                     /locus_tag="PLP95_10015"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HPB46175.1"
                     /translation="MTPPREGGPDGHTPQDHRHVWPTVRGFAATRLLGNLFVRAPYVF
                     ITEISRGLGVSVETMTVVLGVRELGGLGSPVAGRLVDNGHGAAVIAAGGVLVGISCLL
                     AATPWFPVFVVVMVLGAFAKNSIDLAQNAWVGHEVPVTKRARVIGFIETTWAGSFLLG
                     VPIIGFATDRFGWRSGFLITGPILALLAVGSGASLRR"
BASE COUNT          411 a          868 c          890 g          495 t
ORIGIN      
        1 acgaccggac ggtcattgat ggagttcgcc ttctcgatcg ccggagcgac ctcctcgggc
       61 gactcgacgc gcatggccac acagcccatc gcctcggccc acttcacgta gtcgggcagg
      121 tccggcgaca ggtagacctc ggagtagcgc tcttcgtaga acatctcctg ccactggcga
      181 accatgccga gatatgcgtt gttgaggatg gcgatcttga ccggaatccg ttcggtcgcg
      241 gccgtcacca tctcctgggc ggtcatctgg aaacagccgt cgccatcgat ggcccacacg
      301 gtgcgatccg gcatgccgac cttcgcgccg atggcggcgg gaaccgaaaa gcccatcgtg
      361 ccgagtccgc ccgagttgat ccaggtgtac gggtactgga aattccagta ctgggcagtc
      421 cacatctggt gctggccgac ccccgacgcg acgatcgtgt cgcgcggcga caggtcgcgg
      481 atctgttcga ggacgaactg cggcttgagc aatccccccg gagcggactg ctcgtaggtg
      541 agcgggtatt tctcctgcca gcccgacagc gtcgaacgcc atggggtgcg atcggtctgg
      601 ttgtcgggag cgccctggtt acgaagcacc cggatgagct cttcggcgac gaggcgggcg
      661 tcgcccacga tcggaacgtc cggccgccgg accttgccca actcggccgg gtcgatatcg
      721 atgtggatga tcttcgcatc gggcgcgaag ccgtcgagtt tgccggtgac ccgatcgtcg
      781 aatcggctgc cgagcgcgat caacaggtcg gactcctgca tggcggtgac cgcggtgtag
      841 ttgccgtgca tcccgggcat cccgaggttc aactcgtgat cgtcgggcag cgcaccgcgc
      901 gccatcaacg tcgtcacgac cggaatgtcg gccagttcgg ccaactcccg cagggcctcg
      961 gcaccgcggg ctttcaacac tccgccgccc acatagagca ccggacggtg ggcagcggtg
     1021 atgagctcgg cggcctcctt gatcttgcgg gggtgaccct tggtcgtcgg cttgtaaccg
     1081 ggcagcgacg cggccacctc ctcgtcggtc ggccaatgcc actccatcat cgagttcgga
     1141 tgcgtcggat cgatgatgtc tttgggaatg tcgagcagga ccgggcccgg atggccggtg
     1201 gtcgccaggt gaaacgcctg gcgcaccgcg agcgggatgt cttgggcgct ggtgaccagt
     1261 tcattgtgct tggtgaccga tcgggtgatt ccgatcgtgt cgcactcctg gaacgcgtcg
     1321 gtgccgatgg ccgcgacgga gacctgaccg gtgatgacca ccatcgggac cgaatccatg
     1381 aacgcgtcgc acagcggcgt cacgatgttg gtggccgccg gccccgaggt caccatcgcc
     1441 acaccgggac gtccagtggc gtgggcatag ccctcggcca tatgcccggc gccctgctcg
     1501 tgtcggacga gcacgtggcg aatggggctg tcgatgatcg ggtcgtacac gggaaggatg
     1561 catcccccgg gcaatccgaa gatcacctcg acgccctcga gctcgagcgc cttgatcagc
     1621 gcctccgccc cggtcattcg ggtcggttgt tctccctgtt gttcacccat ggatcttctc
     1681 tctgacagtt gatgtcggat cgtgacaagt ttggtcgttc gccggcctcg gcgtcagttc
     1741 ctgcgctgcc tcccactcgt cgttgcgacc cgcttcgggt cgatctccca catcggcggg
     1801 agccggtcgg accgatctcg gatcgcccat ccgaaaaaga cgaaaggccc ccggagggac
     1861 cttggacgcg cacgcaggaa tatcggctgc ggcgctagcg cactactacg aggctggagg
     1921 aaacgatcag gtgcgtcacg gccacaacga tatcagcaac acgcggccgt gggcgccgtt
     1981 tcgggttgag agccggggcg gcacggtcgc gaccggtagc ctgcatgcca tgccgcggtc
     2041 cccgatcgac actcagcgct gcgccgatcg gcgatgacgc cgccgcgtga gggcggcccc
     2101 gacggtcata ccccgcagga tcatcgccac gtgtggccga cggtgcgtgg cttcgccgcc
     2161 acccgcctcc tcggcaacct gttcgtacgg gcgccgtacg tgttcatcac cgagatctcc
     2221 cggggcctcg gtgtcagcgt cgaaaccatg accgtcgtgc tgggcgtgcg cgagctcggc
     2281 gggctggggt cgccggtcgc cggtcgactc gtggacaacg gccacggtgc tgcggtcatc
     2341 gccgcgggtg gagttctggt cgggatctcc tgtctgttgg ccgccacccc gtggtttccc
     2401 gtgttcgtcg tcgtcatggt gctgggggcg ttcgcgaaga actcgatcga cctggcccag
     2461 aacgcgtggg tcggccacga ggttcccgtc accaaacgag cgcgggtcat cggtttcatc
     2521 gaaacgacct gggcggggtc gttcctcctc ggcgttccga tcatcggctt cgccaccgac
     2581 cgtttcggct ggaggtccgg gtttctgatc accggaccga tcctggcgtt gttggcggtc
     2641 ggttccgggg cgagcctacg gcgc
//
LOCUS       DAOOMA010000384         2656 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_47371_length_2656_cov_3.450211, whole genome shotgun sequence.
ACCESSION   DAOOMA010000384 DAOOMA010000000
VERSION     DAOOMA010000384.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 2656)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2656)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2656
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47371_length_2656_cov_3.450211"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..330
                     /gene="lepB"
                     /locus_tag="PLP95_10020"
     CDS             <1..330
                     /gene="lepB"
                     /locus_tag="PLP95_10020"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:TIGR02227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HPB46176.1"
                     /translation="AAVNNDASQPAQLIKRVIGLPGDVVEARGGIVYIDGEPIDESAY
                     LSPDVITNNLAQPVTVAPGQIFVMGDNREHSMDSREFGTVSESSVIGRAVVIVWPIGR
                     WGGFPGD"
     gene            456..1040
                     /gene="lepB"
                     /locus_tag="PLP95_10025"
     CDS             456..1040
                     /gene="lepB"
                     /locus_tag="PLP95_10025"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:NF021981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HPB46177.1"
                     /translation="MTTSEQSTLDGSRSSSPHEKPSRASSAKSWTITIALAVGLTILL
                     RMTLFQAYSIPSPSMVPTLNVGDRVLVWYASKHPSRGDIIVFNRPPLNPKMSADEPDV
                     LIKRVIGLPGETVTAVDGVVYIDGNRLEESYLPDGTQTIIDAPITVPSDEYLVLGDNR
                     LQSVDGRRFGTISQELIVGRAVLRIWPPNRLGGL"
     gene            complement(1074..1811)
                     /locus_tag="PLP95_10030"
     CDS             complement(1074..1811)
                     /locus_tag="PLP95_10030"
                     /inference="COORDINATES: protein motif:HMM:NF015000.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HPB46178.1"
                     /translation="MIPKQLPVGETALLFADRGASAQLGENTIESFAVARRLGATAIE
                     SDLHSSADGFALLRRSPRTAGMRRRRVAEVSRSQLGDSVVTLEEFYEQVGDDLDILLH
                     VHDADAVEAALHSATRFRALDRLWLASADLEALAQWRERSELVRLVHEIGPETFAGGA
                     ERHAAALRSQRVDATMADRSLWNGGRVALYHRFTRRCFAQNAVHERMAVTLFHIGLDG
                     VSSAHPDRLVDAHREVTRPDAPPVRED"
     gene            1906..2232
                     /locus_tag="PLP95_10035"
     CDS             1906..2232
                     /locus_tag="PLP95_10035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB46179.1"
                     /translation="MALIRATCSDCGDVELRSRDIQVRICRDNGNGSYLFRCPVCRMT
                     EVKSADDQIVDILVAAGVRCSEWQLPAELLERPGGDAITHDDVLDFHELLNSEHWYAE
                     LESLQG"
     gene            2326..>2656
                     /locus_tag="PLP95_10040"
     CDS             2326..>2656
                     /locus_tag="PLP95_10040"
                     /inference="COORDINATES: protein motif:HMM:NF014470.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HPB46180.1"
                     /translation="MTSGGLLGILDSLKSGEVLMILVLAMVVLGPERLPQTARDIGRW
                     IAKFRSASSGITSELREVMNDPDMAPLREVGEFVASPRRKIMEYATEAENEAAEARRL
                     ADRAAEAA"
BASE COUNT          482 a          876 c          847 g          451 t
ORIGIN      
        1 gccgccgtga acaacgacgc gtctcagccg gcgcagctca tcaagcgcgt catcggcctg
       61 cccggtgacg tggtcgaggc cagaggcgga atcgtctata tcgacggcga gccgatcgac
      121 gagagcgcct acctgtcgcc cgatgtgatc accaacaacc tggctcagcc ggtgacggtg
      181 gcccccggac agatcttcgt gatgggtgac aatcgagagc acagcatgga cagccgagaa
      241 ttcggcaccg tgtcggagtc ttcggtgatc gggcgtgccg tggtcatcgt gtggccgatc
      301 ggacggtggg gcggctttcc cggcgattga cccgctgcga gggccgggca tcgaactcac
      361 cggtgccctg ccgatcgccg cctgggcccg attcatccgc cacggaacga accggtagga
      421 tgccgatccc acccgcattc acccgagcgg agcccatgac cacctcggaa cagtccaccc
      481 tcgacggctc acgctcgtcc tctccgcacg aaaagccgag ccgggcaagc tccgcgaaga
      541 gttggacgat caccatcgct ttggccgtcg ggttgacgat cctgttgcgg atgacgctct
      601 tccaggcgta ttcgattccc tcgccgtcga tggtgcccac gctcaacgtc ggcgatcggg
      661 tgctggtctg gtacgcatcc aaacacccga gtcgcggcga catcatcgtg ttcaaccggc
      721 cgccgctcaa ccccaagatg agtgccgacg aacccgacgt cttgatcaag cgggtcatcg
      781 gactcccggg cgaaaccgtc accgcggtcg acggggtcgt ctacatcgac gggaaccgac
      841 ttgaggagtc gtacctgccc gacggcactc agacgatcat cgacgcaccg atcacggttc
      901 cgtccgatga atacctggtg ttgggggaca atcgtctgca gtcggtcgac ggccgtcgat
      961 tcggcacgat ctcgcaggaa ctcatcgtcg ggcgtgcggt gttgcggatc tggccgccga
     1021 atcgactggg cggcctgtag ccacggcact cgccagcggc actagtgccg gcactagtcc
     1081 tcgcgcaccg gtggggcgtc ggggcgggtg acctcacgat gcgcgtcgac gagacggtcg
     1141 gggtgcgcgg acgacacgcc gtcgagtccg atgtgaaaca acgtcaccgc catgcgttca
     1201 tgcacggcat tttgagcgaa acaccggcgg gtgaaccggt ggtacagcgc gacgcggcct
     1261 ccgttccaca acgagcgatc ggccatcgtg gcatccaccc gctgcgatcg aagcgccgcg
     1321 gcgtggcgtt cggctccgcc ggcgaaggtt tcggggccga tctcatgcac gagacgaacg
     1381 agttcgctgc gttcgcgcca ctgcgccaac gcctcgaggt ccgcgctggc gagccacagc
     1441 cgatcgagcg cccgaaaccg ggtggcgctg tgcaacgccg cctcgaccgc atcggcatcg
     1501 tgcacgtgca acaaaatgtc gagatcgtcg ccgacctgtt cgtagaactc ctcaagggtc
     1561 accacgctgt cgcccagttg cgacctcgac acctcggcca ctcgacgacg tcgcatgccg
     1621 gcggttcgcg gcgacctgcg caacaacgcg aacccatcag cggaggaatg cagatccgac
     1681 tcgatcgccg tcgctccgag acgccgtgct acggcaaagc tctcgatggt gttctcgccc
     1741 aactgcgcgc tcgcgccgcg atcggcgaac aaaagggccg tttcgccgac cggtagctgc
     1801 ttgggaatca tgtaggccga ccgaacttca cggcctgaaa tgttaactcc atgtcctggc
     1861 gtgccgaaga acagaggaag ccacccagtg aacgagggag ttcccatggc actcatccgt
     1921 gcaacgtgca gcgattgtgg agacgtggag ttgcgttccc gcgacatcca ggtccggatc
     1981 tgtcgcgaca acggcaacgg gagttacctg ttccgctgcc cggtgtgtcg catgaccgag
     2041 gtgaagtccg ccgacgacca gatcgtcgac atcctggtcg ccgctggggt tcgctgcagc
     2101 gagtggcagc ttcccgccga actgcttgag cgccccgggg gcgacgcgat cacccacgac
     2161 gacgtcttgg acttccacga attgttgaac tccgaacatt ggtacgcaga actcgagtcg
     2221 ctccagggct gaggtcggtc tgacaccgac gtttgtgcgg cgacgtttgt gcggcgaccc
     2281 tgtggggcgc atttgcgccg cagcgacgcc ctgtagcatc tggccatgac ctctggaggc
     2341 ctactcggca tcctcgacag cctcaagagc ggcgaggtcc tcatgatcct cgttctcgcg
     2401 atggtcgtgc tcggccccga acggctacct caaaccgcac gtgacatcgg ccgttggatc
     2461 gccaaattcc gttcggcgtc ttcgggcatc accagcgagt tgcgcgaggt gatgaacgat
     2521 ccggacatgg ctccgcttcg cgaggtgggc gaattcgtcg cgtcgccgcg tcgcaagatc
     2581 atggagtacg cgaccgaggc ggaaaacgaa gccgccgaag cccgccgcct ggccgaccgg
     2641 gccgccgagg cggcgg
//
LOCUS       DAOOMA010000385         2642 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_47719_length_2642_cov_4.941631, whole genome shotgun sequence.
ACCESSION   DAOOMA010000385 DAOOMA010000000
VERSION     DAOOMA010000385.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 2642)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2642)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2642
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47719_length_2642_cov_4.941631"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..639
                     /locus_tag="PLP95_10045"
     CDS             <1..639
                     /locus_tag="PLP95_10045"
                     /inference="COORDINATES: protein motif:HMM:NF022427.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2804 domain-containing protein"
                     /protein_id="HPB46181.1"
                     /translation="AERTAWGPLDLDVAVERPEGHESLNVVIPWSLRRFQYTAKDNSR
                     RVRGHISIGDRRFDLGAEGDETTWGILDVGRGLWRYNNRWNWASGSGIASGGAVVGLQ
                     FGGKWTEGTGYNENALCVDGRLHKIHNELSWDYDWDHPLRPWRVIDPVGGLVEATLSP
                     THDRHSKVSIGVLSMEVHQVFGTWAGTITSESGDLVTFDAVVGFAEEARNRW"
     gene            662..2317
                     /locus_tag="PLP95_10050"
     CDS             662..2317
                     /locus_tag="PLP95_10050"
                     /inference="COORDINATES: protein motif:HMM:NF005863.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HPB46182.1"
                     /translation="MQTGSNGWNIADIWESHAAVVPNAPAIFFGDVELSWADFEDRAA
                     RLATVFNDAGLDRDSKVALYLYNGPAYLDATFAAFKQRMVPVNVNYRYTDAELRYLLD
                     NSDAEAIMVDTRFVDNVARLLPELPKVKVVVEVDEAGVGPNPAGAQIPSLIAHADAVT
                     TPPRAPRIERSLEDLWFLYTGGTTGMPKGVMWPHRSIIGAGATNFRPYATGTPDSIEF
                     FAEVARAAHDGERAPLRLLPAAPLMHGTSALTSWGTLNASGAIVMLTGHSFDAEELIT
                     TIERRGVTNLTIVGDAFARPMLAALDAADERGEPVDLSSLRLVVSSGVMWSQQAKDAL
                     LARADVACADLLGSSEGVGFANSVAKRNRSAPTARFKLGPDAQVFGDSGAPVVPGSGE
                     RGVLAVGGPIPVGYYKDEAKTAETFRTFEGRVWSVPGDFATVEADGTITLLGRGSVCI
                     NTAGEKVFVEEVEETLKRHEAVLDANVIGVPDDKWGNAVCAVVSLGDGTDGDDETAQA
                     LMDHCRETLAGYKVPKRIVFVPTVQRGPNGKADYRWAASAVSG"
     gene            2314..>2642
                     /locus_tag="PLP95_10055"
     CDS             2314..>2642
                     /locus_tag="PLP95_10055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB46183.1"
                     /translation="MKIRPWHGVFAIVLAASAFAIGACSEGEPGGDGGAGGGGEAGGV
                     ADEAEFPEWIDVAPDFLVERLGGPTSAREFRGRPFNQSARSLSDEEFRRFGSGETPRS
                     TTTSPTG"
BASE COUNT          444 a          877 c          901 g          420 t
ORIGIN      
        1 gccgaacgca cggcgtgggg gccactcgac ctcgacgtcg cggtcgagcg acccgagggg
       61 cacgagtcgc tcaacgtcgt catcccgtgg tcgctgcgcc ggtttcagta caccgcgaag
      121 gacaactccc gccgcgtgcg gggtcacatc tcgatcggcg atcgtcgatt cgatctcgga
      181 gccgagggcg acgagacgac ctggggcatc ctcgacgtcg gccgggggtt gtggcgttac
      241 aacaaccgct ggaactgggc gagcggttcg ggaatcgcga gcgggggagc ggtggtcgga
      301 ctccagttcg gcggcaagtg gaccgagggc accgggtaca acgagaacgc cctgtgcgtg
      361 gacggccgcc tccacaagat ccacaacgag ttgtcctggg actacgactg ggaccaccca
      421 ctgcgaccgt ggcgggtgat cgacccggtg ggcgggttgg tcgaggcgac cctcagcccc
      481 acccacgacc ggcacagcaa ggtgtcgatc ggagtgttgt cgatggaggt ccatcaggtc
      541 ttcggcacct gggcgggcac gattacctcc gagtccggcg acctcgttac ttttgacgcc
      601 gtcgtcggtt ttgcggaaga agcgcgcaac cggtggtgac gaagcgctga acggagcaga
      661 catgcaaacg gggtcgaacg ggtggaacat cgccgatatc tgggagtcgc acgcagcggt
      721 cgtgccgaac gcccctgcga ttttcttcgg cgacgttgaa ctgagctggg ccgacttcga
      781 ggatcgcgcg gcccgactcg ccaccgtgtt caacgacgcc ggactcgacc gggactcgaa
      841 ggtcgcgctg tacctctaca acggccccgc ctacctcgat gccaccttcg ctgcattcaa
      901 gcagcgaatg gttccggtga acgtgaacta ccgctacacc gacgccgaac tgcggtacct
      961 gctcgacaac tccgatgctg aggcgatcat ggtcgacacc cgattcgtcg acaacgtcgc
     1021 ccgcctgttg ccggagttgc cgaaggtgaa ggtcgtcgtc gaggtggacg aggccggcgt
     1081 cggacccaac cccgccggcg cgcagatccc cagtctcatc gcccacgccg acgcggtgac
     1141 gaccccgccc cgggcgccac gaatcgaacg cagtctcgag gacctgtggt tcctctacac
     1201 cggtggcacc accggaatgc ccaagggcgt catgtggccg caccggtcga tcatcggggc
     1261 cggcgccaca aactttcggc cctacgccac gggaaccccc gactcgatcg agtttttcgc
     1321 cgaggtcgcc cgagcggccc atgatggcga gcgcgcgccg ctgcgcctgt tgcctgccgc
     1381 accgctgatg cacggcacct cggcgctgac cagctgggga acgctcaacg ccagcggggc
     1441 catcgtgatg ctcacgggcc acagcttcga cgccgaggaa ctgatcacca cgatcgaacg
     1501 tcgcggtgtc accaacctca ccatcgtcgg cgacgcgttc gcccggccca tgctcgccgc
     1561 gctcgacgcc gccgacgaac gcggggagcc ggtcgacctg tcgagcctgc gactcgtggt
     1621 cagctcgggg gtgatgtgga gccaacaggc caaggacgcg ctcctcgcca gagcagacgt
     1681 cgcatgtgcc gacctgctcg gcagttccga gggggtcggt ttcgccaatt cggtcgccaa
     1741 acgcaatcgc tcggccccga ccgcccgttt caagctcggc cccgacgccc aggtgttcgg
     1801 cgattccggt gccccggtcg tgccggggtc gggggaacgg ggcgtgctgg cggtcggcgg
     1861 gccgattccc gtcggctact acaaggacga ggccaagacc gcggagacgt ttcgcacctt
     1921 cgagggccgc gtctggtcgg tgcccggcga cttcgccacg gtcgaagccg acggcaccat
     1981 caccctgctc ggacgcggct cggtgtgcat caacaccgct ggcgagaagg tgtttgtcga
     2041 ggaggtcgag gagaccctga aacgccacga agcggtgctc gacgccaacg tcatcggcgt
     2101 gccggacgac aagtggggca acgccgtctg tgcggtggtc agcctcggcg acggaaccga
     2161 cggtgacgac gagacggcac aagcgctcat ggaccactgc cgtgaaaccc tcgcgggtta
     2221 caaggtgccc aaacgcatcg tgttcgtgcc gacggtgcaa cgcggcccca acggcaaggc
     2281 cgactaccgc tgggcggcat ccgcggtctc ggggtgaaga ttcgtccgtg gcacggcgtc
     2341 ttcgcgatcg tgctcgccgc gagcgcgttc gcgatcggcg cctgttccga gggtgaaccc
     2401 ggcggcgacg gtggcgctgg tggaggtggt gaggccggcg gtgtcgccga cgaggccgag
     2461 tttcccgaat ggatcgatgt cgccccggac tttctcgtcg aacgactcgg cggcccgacc
     2521 tcggctcgcg agtttcgcgg ccggccgttc aaccagtcgg cgaggtcgtt gtcggacgag
     2581 gagtttcgtc gcttcggtag cggggaaact cctcgttcga caacgacctc gccgactggt
     2641 tg
//
LOCUS       DAOOMA010000386         2641 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_47744_length_2641_cov_4.428074, whole genome shotgun sequence.
ACCESSION   DAOOMA010000386 DAOOMA010000000
VERSION     DAOOMA010000386.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 2641)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2641)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2641
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47744_length_2641_cov_4.428074"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(167..1033)
                     /locus_tag="PLP95_10060"
     CDS             complement(167..1033)
                     /locus_tag="PLP95_10060"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HPB46184.1"
                     /translation="MPTTIHVSHATRRYGPTTVLDDVSFDLAPGITALLGPNGAGKTT
                     LIGLLSTASRPDAGEITVFGHRSDGPLPERIEIRRRLGFLPQEVRFPAGMTAFGFLDY
                     IAVLKEWDDTGARHAEVRRVLSVVGLDDRPTIKVRKLSGGQRRRLAFAQALIGDPDLL
                     ILDEPTTGLDPEQRARFRATLSERARHGTVLLATHQTEDVAAVCDRVIVLVAGRVAHD
                     GDVAGFVATARNRVWLGDRPVADAVATWRDPGGGVRSVGGTPPPGAEAAPPTVEDAYL
                     LLVGPPAMEGAA"
     gene            complement(1044..1892)
                     /locus_tag="PLP95_10065"
     CDS             complement(1044..1892)
                     /locus_tag="PLP95_10065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB46185.1"
                     /translation="MSAWHVDDAVVTAYGERRLDAVQRASVEAHAARCQECRDILGTR
                     VVGDQRSPALDLDRLWTRVESAVSTPKVGRADRALLRLGLSMSDVVVTRVVAAQSRQW
                     TIATTLVLAVTAVTAVLGPVDSAHTAFLVVAPLLPALGVAAIYRLVPHGIDMLERAAP
                     YSPARLLLWRTAYVVVTAVPISVAAGVAVLQHPWAAVSWLLPALGCTLCVVAAASWHD
                     PVRPAAAVAMGWTAVVVTWQVRDTPWAIATAPTQLVAAAIAAAAALVIHHRLSNDPTR
                     PVFGGP"
     gene            complement(1889..2467)
                     /locus_tag="PLP95_10070"
     CDS             complement(1889..2467)
                     /locus_tag="PLP95_10070"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HPB46186.1"
                     /translation="MSVSGSSGDQRDDVALVAAVAGGDAAALRVLYDRHAPWLVARLR
                     ARCWDDALIADAVQDTFLAVWRGAARWRADGDVGAWIWGIGVRQLVTHLRRAGRQARP
                     APFRERAAAPSTEERVLESIDLGEAGVVLASLSPELMEVVQAMVFDGLTAKETGRMLG
                     VPEGTVKGRMRKAKSVLRTELAGTTLHLGDLR"
BASE COUNT          437 a          906 c          915 g          383 t
ORIGIN      
        1 cctcgggttg tcgccttgtc gccgtcgagg taggggagga gcgaggcgta ggtgagcgcc
       61 aacccgagga ggaacgcagg ccgacccagg ttgcgtcgaa tctcgatccg tgccagctgt
      121 gcccacggcg accggaccgg cgcgatcgcg gaggtctggg tggcggtcat gccgcacctt
      181 ccatcgcagg cggaccgacg aggagcaggt aggcgtcctc gaccgtcggc ggtgcggctt
      241 cggcccctgg cggcggggtg ccaccgacgg agcgcacgcc gccaccaggg tcgcgccagg
      301 tcgccaccgc gtcggcgacc ggtcggtcgc cgagccacac ccgattgcga gcagtcgcga
      361 cgaacccggc gacgtcgcca tcatgggcaa cacgcccggc gacgagcacg atgacgcggt
      421 cgcacacggc ggcgacgtcc tcggtctggt gggttgccaa caacacggtt ccgtggcggg
      481 ctcgttcaga aagcgtggcc cggaaccggg ctcgttgttc ggggtcgagg ccggtggtgg
      541 gttcgtcgag gatcaacagg tccgggtcgc cgatgagcgc ctgggcgaag gccaaacggc
      601 ggcgctgccc gcccgacagc ttgcgcacct tgatcgtggg tcggtcatcc agccccacga
      661 cgctgagcac ccgccgcacc tcggcgtggc gggcaccggt gtcgtcccac tccttgagta
      721 cggcgatgta gtcgaggaat ccgaacgcgg tcattccggc agggaaccgg acctcctgtg
      781 gcaagaagcc cagccgtcgg cggatctcga tccgctcggg caacggtcca tcgctgcgat
      841 gcccgaacac ggtgatctca ccagcatcgg gtcgactcgc cgtgctcaac aacccgatca
      901 gggttgtctt gcccgcaccg ttggggccga gcagcgccgt gatgccaggg gcaaggtcga
      961 agctgacgtc atcgaggacc gtggttgggc cgtagcggcg ggtcgcgtgc gagacatgaa
     1021 tggtggtggg cacgggtcct ccttcagggt ccgccgaaca cgggccgggt tgggtcgttc
     1081 gaaagccggt ggtggatgac cagcgcggca gcggccgcga tcgccgcggc aacgagttgt
     1141 gttggagcgg tagcgatcgc ccacggcgtg tcgcgcacct gccaggtgac cacgacagcg
     1201 gtccacccca tcgcgacggc tgcggcgggg cgcaccgggt cgtgccagct cgccgcggcg
     1261 acgacgcaca acgtgcaacc caacgccggg agaagccagc tcaccgccgc ccatgggtgc
     1321 tgcaggaccg ccacgccggc ggcgactgag atcggcacgg cggtgaccac cacgtacgcc
     1381 gtgcgccaca acaacaggcg cgccggcgag tacggggctg cccgctcgag catgtcgatg
     1441 ccatgcggca ccagccgata gatagctgcg acgccaaggg cgggaagtag cggagccacg
     1501 accagaaacg cggtgtgggc cgagtcgacg ggaccgagaa ccgccgtcac cgcggtgact
     1561 gcgagcacca gcgtggtggc gatcgtccat tggcggctct gagcggcgac gacacgtgtc
     1621 accaccacgt ccgacattga caacccgagc cggagcaacg cccgatctgc gcgcccgacc
     1681 ttcggagtgg agactgcgga ctcgacccgc gtccacaagc ggtcgaggtc gagagcgggt
     1741 gaacgctggt cgccgaccac gcgggtgccc aggatgtcgc ggcactcctg gcacctggcc
     1801 gcgtgcgctt cgaccgacgc ccgctgtacg gcatcgagtc tgcgttcgcc gtaggcggtc
     1861 acgacggcat cgtccacgtg ccaggcgctc accggagatc tcccaggtgc aaggtcgtgc
     1921 cggcgagttc ggtgcgcagg acactcttgg ccttgcgcat ccgccccttc acggttccct
     1981 ccgggacacc gagcatccga cctgtctcct tcgccgtcag accgtcgaag accatcgctt
     2041 ggaccacctc catcagctcg ggcgacagcg acgccaacac gacgcccgcc tcgccgaggt
     2101 cgatcgactc cagaacccgt tcctccgtac ttggcgcggc agctcgctcc cggaacggag
     2161 caggtcgggc ctgacgtccg gcccggcgca ggtgggtgac gagctggcgc acaccgattc
     2221 cccagatcca cgcaccgaca tcgccgtcgg cgcgccaccg cgcggcaccg cgccacaccg
     2281 ccaagaacgt gtcctgaaca gcgtctgcaa tcagggcatc gtcccaacag cgggcgcgaa
     2341 ggcgggcgac caaccacgga gcgtgtcggt catagagcac ccggagcgca gcggcatcac
     2401 caccagcgac tgcagcgacg agggccacgt cgtcgcgctg gtctccggaa ctcccggaca
     2461 cgctcatcgt cgtctccccg attggctcat cgtttcactg tcgttcgaac gcagcgtttc
     2521 agatcatggc gtgttcgctg ggtgttgtcg tgcccgcttc gaggctttga ttaaggcgtg
     2581 atcgtgggcc gcctcgccga tctcctgagt gaaccggtga tcctcgcgga cgaggtcggg
     2641 g
//
LOCUS       DAOOMA010000387         2638 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_47813_length_2638_cov_4.613240, whole genome shotgun sequence.
ACCESSION   DAOOMA010000387 DAOOMA010000000
VERSION     DAOOMA010000387.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 2638)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2638)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2638
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47813_length_2638_cov_4.613240"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..388)
                     /locus_tag="PLP95_10075"
     CDS             complement(<1..388)
                     /locus_tag="PLP95_10075"
                     /inference="COORDINATES: protein motif:HMM:NF021608.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent metalloprotease"
                     /protein_id="HPB46187.1"
                     /translation="MTHFDGADDDTPDPLGDLAAGLNQIFSLFGAGAGGPGAAGLAGL
                     GGALGSPNWEQARQSAAALATQGHSDANVDPLVRMQYEQLARVAELQVARVTGLSLTR
                     SGAALAVTPVNRSQFARDTVDDYRPLF"
     gene            complement(451..1542)
                     /locus_tag="PLP95_10080"
     CDS             complement(451..1542)
                     /locus_tag="PLP95_10080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB46188.1"
                     /translation="MGVAQPPEDDRGDSALPPAHTRPWRHPSELGRFHAPGASPGPAS
                     VRVGPSSAFRSKAASAEGAGRRFSAGSLVVAAALGAVTLFATMTALGVVNPAVPQSSP
                     GSGQTASDVGRIAGTATVRVEYSGSVRVGTGVLVEPDMAITALPATAIDSGQWSYRRD
                     GTWGEASLVGIDPTTGLTLVSFAEPVAETPGVVDRGADTSDSDTVDVATEAADPTTAS
                     SAPASSPATSSVATPSVATPEIEAPEIEAPWAAASLGDSVRVAAASSGLPTLNVRGRT
                     QRIVSESGRITSGSSTLDHLLVVTAQRGTAAGDLLLHTDHDTIVGLILDRDDDSNDHL
                     AFAVPTQRAVVIARNLAQAAAARPPDTAA"
     gene            2311..>2638
                     /locus_tag="PLP95_10085"
     CDS             2311..>2638
                     /locus_tag="PLP95_10085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HPB46189.1"
                     /translation="MPRDLAIDLGTANTLVYARGEGIVLNEPSVIALNSRNREVLAMG
                     REAWQMIGRTPSYIVAVRPLRKGAITDFEVTQEMMRLLLKRVGVSRFNRPRVVVCVPS
                     AITAVER"
BASE COUNT          407 a          899 c          924 g          408 t
ORIGIN      
        1 cgaacagcgg ccggtagtca tcgacggtgt cgcgggcgaa ctggcttcgg ttcaccgggg
       61 tgacggcgag cgccgcgccc gatcgggtca ggctcagccc ggtcacccgt gccacctgga
      121 gttcggcgac gcgagcaagc tgttcgtact gcatgcgcac gagcgggtcg acgttggcgt
      181 cgctgtgccc ctgcgtcgcc aacgctgccg cggattggcg ggcctgctcc caattcggcg
      241 agccgagtgc tccgcccaag ccggccaacc ccgcagcacc ggggccgccc gcccccgcgc
      301 cgaacaggga aaagatctgg ttgaggcccg ccgcgaggtc gccgagcggg tcgggggtgt
      361 cgtcgtcagc gccgtcgaag tgggtcacgg caacatggta gatccctcgg cgaacggtcg
      421 atcggcgccc acgaggagcg ctctcaggcg tcaggccgcg gtgtcgggcg gacgtgccgc
      481 tgctgcctgt gccaggttcc tggcgatcac caccgcccgt tgggtcggga cggcgaaggc
      541 cagatgatcg ttcgaatcgt cgtcgcgatc gaggatcagg ccgacgatcg tgtcgtggtc
      601 ggtgtgcagg aggaggtcgc ctgcagccgt gccccgctgg gcggtgacga ccagcagatg
      661 atcgagggtg gacgagccgc tggtgatccg gcccgactcg ctcacgatcc gctgggttcg
      721 gcctcgcacg ttcaacgtcg gcagcccgga cgacgcggcg gcgacacgca ccgagtcgcc
      781 cagcgaagcg gccgcccacg gcgcttcgat ctctggtgcc tcgatctccg gcgtggcgac
      841 cgatggcgtg gcgaccgatg acgtggcggg ggaggacgct ggcgcagatg aagctgtggt
      901 tggatcggcg gcctcggtag cgacatcgac ggtgtcggaa tctgaggtgt cggctccgcg
      961 gtcgacgaca ccaggggttt ccgccacggg ttcggcgaac gacaccaacg ttagaccggt
     1021 cgtcgggtcg attccgacga ggctcgcctc accccaggtt ccatctcgtc gatacgacca
     1081 ttgccccgag tcgatcgccg tcgccggcaa cgcggtgatc gccatgtcgg gttccacgag
     1141 gacgccggtt cccacccgca ccgatccgga gtattcgacc cgaaccgtcg cggttccggc
     1201 gatacggccg acgtcggagg ccgtctggcc gctgccggga gaggactgcg gcaccgcagg
     1261 gttgaccacg ccgagtgccg tcatcgtggc gaagagggtg accgcgccga gagcggcggc
     1321 cacgacgagc gaaccggccg agaaccgacg gccggcgcct tcggccgagg ccgccttcga
     1381 acggaacgcg gacgacgggc caaccctgac cgacgctggg ccgggggaag cgccgggcgc
     1441 atggaaccgg ccgagctcgg agggatggcg ccaaggtcgg gtgtgtgccg gcgggagcgc
     1501 tgaatcgccg cggtcgtcct ctggcggttg tgcgacaccc atgacggtga ccttaccgag
     1561 cattgcccgg atcgagaccg cgggcccgat tgcgcgactc cggttcgggc gagtcgagga
     1621 aaggaacccg ccggcacggt tggcgggcgt cgaatcgtgc aagatagagc cgttcgctcc
     1681 tgtgccctgt cgacgggcca tcgacgtcgc ttcctgcctt gcaccaatgt tccacgtgtc
     1741 ctgtcaccca ccctccgatc gtgaaatccg gctcgtcacg ccctcgacgc ttgggggacc
     1801 tgagccgtca tgagtccggt gtcctacctc ggtattgctc tggtgatcgc cgtcgtcggc
     1861 ggaatgatcg tcgcgttggc cctgcgggaa cctcgcacac ggcgaggtga cggtgtcgat
     1921 gccttccgcc gtagcatgga tgcgttggcg cccgggaacg actcggcgtc atccccagtg
     1981 gttgtgcgca aggctgcgca gccgcggggt cgaaccgtta ccgaatccgc cgacgagccc
     2041 gaccccgccg tccccgccgt tgaagggggc gtcgatcgcc ccgaaccgcc cccgacaccg
     2101 ccgacggtgt cacaatcgtc tcacgactcc gttgccgatg acctcgattc caaccatcca
     2161 gcgtccgacc cgcagccccc tccgaccgtc tcggagccct cggacgcgtc ccgggccgaa
     2221 ccggcaaccc cgcccgatac cagcctcgag cctgacgccg gggagtctga agtcgtcgag
     2281 tctgaagctg tcgagcggga ccgctgagta atgccccgcg acctcgcgat cgacctcggc
     2341 accgccaata ccctcgtgta cgcgcgcggc gaaggcatcg tgttgaacga accgtcggtg
     2401 atcgcgctca acagccgcaa ccgcgaagtg ctcgccatgg ggcgcgaggc gtggcagatg
     2461 atcggccgca ccccctccta catcgtcgcg gtgcggccgt tgcgcaaggg cgccatcacg
     2521 gacttcgagg tcacgcagga gatgatgcgc ctgctgctca agcgagtcgg ggtgagccga
     2581 ttcaaccggc cgcgggtcgt ggtgtgcgtg ccctcggcga tcactgcggt cgaacgtc
//
LOCUS       DAOOMA010000388         2636 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_47864_length_2636_cov_4.681519, whole genome shotgun sequence.
ACCESSION   DAOOMA010000388 DAOOMA010000000
VERSION     DAOOMA010000388.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 2636)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2636)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2636
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47864_length_2636_cov_4.681519"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            59..1693
                     /locus_tag="PLP95_10090"
     CDS             59..1693
                     /locus_tag="PLP95_10090"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004333 - fumarate hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase"
                     /protein_id="HPB46190.1"
                     /translation="MADFDFSELLPLGDDPTPYRLISTEGVSTFEANGNTFLQVEPEA
                     LTLLTQTAMRDIAHLLRPGHLQQLRNICDDPEASANDYFVALELLKNANIAAGGVLPS
                     CQDTGTAIVKGKKGQFVIVNGGHDEQHLARGVFNTYQTSNLRYSQMAPLNMWDEVNTG
                     NNLPAEIKISSIDGNAYKFLFMAKGGGSANKSYLFQETKALLNPKSLAAFLDKNLRNI
                     GTSACPPYHLAIVVGGTSAEFALETAKLASARYLDSLPTEGSKLGHGFRDLDMEQEVL
                     EITRNFGIGAQFGGKYFCHDVRVIRLPRHGASCPVAVAVSCSADRQALAKITTEGIFL
                     EQLETDPAHYLPDPHEGQIGGEVVSIDLNRTMDEIRQELSKYPVKTRLSLTGTLVVAR
                     DIAHAKIAERLDAGEPMPQYLRDHAVYYAGPAKTPEGMASGSFGPTTAGRMDSYVDKF
                     QAAGGSFVMLAKGNRSQQVTDACQAHGGFYLGSIGGPAARLAKDSIRKVEVLDYEELG
                     MEAVWRIEVEDFPAFIVVDDKGNDFFADVSTPVTLK"
     gene            complement(1765..>2636)
                     /locus_tag="PLP95_10095"
     CDS             complement(1765..>2636)
                     /locus_tag="PLP95_10095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB46191.1"
                     /translation="DRAPSDASGEELAAGVVQFETVASDSSASDASTSDASATVSEPT
                     EVAAAPTAVAAASAELDALRSQIASLQDSIDELSSRPSVETIEPEYLGASKTLRYAQQ
                     TADAIIADARTRSEELVADAQRQRTLIIRQGREQAEAEFGEERDRVRGEAAAWEARRA
                     ELFEELASLTAVIGRYHDGLRSLEDHLDGAVETLKGGVDAPQTEADAPQADGDAEAEA
                     DSVAHEVGDDVVATTDEADGSELDERKAATDDSAASSASSASMTPPPVPGAFLGESES
                     TRADGPAARFSTD"
BASE COUNT          458 a          952 c          808 g          418 t
ORIGIN      
        1 caggtagatc actgtcgccg aatcgcacct gcgctcggcg cggatctagc gttgacccat
       61 ggccgacttc gacttttccg agctgcttcc gctcggcgac gatcccaccc cgtacaggct
      121 catcagcacc gagggtgtca gcaccttcga ggcgaacggc aacacgttcc ttcaggtcga
      181 acccgaggcg ttgaccctgc tcacccagac cgccatgcgc gacatcgcgc atctgttgcg
      241 gcccggacac ctccagcagc tccgcaacat ctgcgacgac ccggaagcga gcgcgaacga
      301 ttacttcgtc gccctcgagc tgctcaagaa cgccaacatc gcggcaggcg gggtgttgcc
      361 ctcgtgtcag gacaccggca ccgcgatcgt caagggcaag aagggacagt tcgtgatcgt
      421 caacggcggc cacgacgagc aacacctggc ccgcggcgtg ttcaacacgt atcagacctc
      481 caaccttcgc tacagccaga tggcgccgct gaacatgtgg gacgaggtca acaccggcaa
      541 caacctgcca gcggagatca agatctcctc catcgacggc aacgcctaca agttcctgtt
      601 catggccaaa ggtggcggct ccgccaacaa gagctacctg ttccaggaga ccaaggcgtt
      661 gctcaacccc aagagcctcg ctgcgttcct cgacaagaac ctgcgcaaca tcggcacctc
      721 agcctgtccg ccctatcacc tcgccatcgt cgtcggcgga acctcggcgg agttcgcgct
      781 cgagaccgcc aagttggcct ccgcccggta cctcgattct ctgccgaccg aagggtccaa
      841 gctcggccac gggttccgcg acctcgacat ggaacaagag gtcctcgaga tcacccgcaa
      901 tttcggcatc ggtgcccagt tcggcggcaa gtacttctgc cacgacgtcc gcgtcatccg
      961 gctcccccgc cacggcgcct cgtgccccgt ggccgtggcg gtgtcgtgtt cggccgaccg
     1021 ccaggcgctc gccaagatca ccaccgaggg gatcttcctc gagcagctcg aaaccgaccc
     1081 ggcgcactac ctgcccgacc cgcacgaagg ccagatcggc ggcgaggtcg tgtcgatcga
     1141 cctcaaccgc accatggacg agatccgcca ggagctgtcg aagtacccgg tcaagacccg
     1201 cctgtcgctc accggaaccc tggtggtcgc ccgcgacatc gcacacgcca agatcgccga
     1261 acgcctcgac gccggcgaac cgatgccgca gtacctgcgc gatcacgcgg tgtactacgc
     1321 cggaccggcc aagacccccg aagggatggc ctcggggtca ttcggcccga cgaccgcagg
     1381 ccggatggat tcctacgtcg acaagttcca ggcggccggt ggcagctttg tgatgttggc
     1441 caagggcaac cgcagccaac aggtcaccga cgcgtgccag gcccacggcg ggttctacct
     1501 cggctcgatc ggtggcccgg ccgcacgcct cgccaaggac tcgatccgca aggtcgaggt
     1561 tctcgactat gaggaactcg gcatggaggc cgtgtggcgc atcgaggtgg aggatttccc
     1621 cgccttcatc gtcgtcgatg acaagggcaa cgacttcttc gccgacgttt ccaccccggt
     1681 gacgctcaag taggcatcgc ccgggaatct tcggcgaccg gccccgaaat cgccgccacg
     1741 cccgcaggcg cgggcgtggc gcggtcagtc ggtgctgaag cgggcggccg gtccatcggc
     1801 ccgagtcgat tccgactcgc ccagaaacgc ccccggcacc ggtggaggcg tcatcgacgc
     1861 tgacgaggca ctggacgcag ccgaatcgtc ggtcgccgcc ttgcgctcgt cgagttccga
     1921 accgtctgcc tcatcggtcg tcgcgaccac gtcgtcgccc acctcatgag cgacggaatc
     1981 ggcctcggct tcagcatccc cgtcggcctg gggggcgtcg gcctcggttt ggggcgcgtc
     2041 gacaccaccc ttgagcgtct ccaccgcacc gtcgaggtgg tcctcgagcg accgcaggcc
     2101 atcgtgatac cgcccgatga ccgcggtgag cgaggccaac tcctcgaaca actcggcacg
     2161 gcgcgcctcc catgctgccg cctcaccgcg cacgcggtct cgctcctcac cgaactcggc
     2221 ctcggcctgt tcgcggccct ggcggatgat cagcgtccgc tggcgctggg catcggccac
     2281 gagctcctcg gagcgggtgc gggcgtccgc gataatcgcg tcggcggtct gttgggcgta
     2341 gcgaagcgtc ttcgacgcgc cgaggtattc gggctcgatc gtctcgacgc tcggacgtga
     2401 ggacagttcg tcgatcgaat cctgaaggct ggcgatctgc gacctcaacg cgtcgagttc
     2461 ggcgctcgcc gcggccaccg cggttggcgc cgcggccacc tcggtcggct ccgagaccgt
     2521 cgccgaggcg tcggacgtcg acgcatcgga ggccgatgaa tccgaggcca ccgtctcgaa
     2581 ctgaaccacc ccggccgcca gctcttcgcc ggacgcgtca gaaggtgccc tgtccg
//
LOCUS       DAOOMA010000389         2633 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_47937_length_2633_cov_3.984872, whole genome shotgun sequence.
ACCESSION   DAOOMA010000389 DAOOMA010000000
VERSION     DAOOMA010000389.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 2633)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2633)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2633
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47937_length_2633_cov_3.984872"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..220
                     /locus_tag="PLP95_10100"
     CDS             <1..220
                     /locus_tag="PLP95_10100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB46192.1"
                     /translation="AFHEPVRRLLALGAANGTLRPVDPEIAATALYGAVTVIGLRSLV
                     IDNHLDVDEVSGQILPLFWSGIRPNTRS"
     gene            complement(376..>2633)
                     /locus_tag="PLP95_10105"
     CDS             complement(376..>2633)
                     /locus_tag="PLP95_10105"
                     /inference="COORDINATES: protein motif:HMM:NF015649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="BTAD domain-containing putative transcriptional
                     regulator"
                     /protein_id="HPB46193.1"
                     /translation="ATEPEIGPYWLEVIDANQDRYVQPGNPNLIHLGQVLVLPPTGLA
                     SLGPPSAEQTAPPPAPAPLPAPAEDEPPVAAPEVLDDTAAPGDVTASTSTATSAPATT
                     AAPTTEAPSTPAPSTGGVEADTEPRDKATGGTLVPVAAGALSSVVLAVGLQRLLARRR
                     RRFANEHPGQISAVTPVGQRELHREVIAHADEDRIDELQQVIGSLAAGLAATASPRRP
                     SVVRHAAGSTEVILDQPDTNPPSGWDTTIDGTVWTFTGTAGIEAFDTVANDDVPSAAP
                     LLVTIGAPEDDAQLYLDLEADGLLALIGDHDVATELARSILTELTLSPLADTIRVVAI
                     GDLVENDVAVLEHLTVADSWDDLTDDLAAWASQSHLSLVENGWQTPFVARAHEPDHDA
                     LSPIVVVADHPPPTELAEMLRAHSPAAVAVVVVGELADVASVRCEPDALYLDHLNLAC
                     TPQRLDSDELAQMAALLAAAEQPADEDSLDEPAAVVPVNVSVPVDPTSGGDPDEPWPV
                     NEPPGYDVLVRLLGDITVDGGAAQLRPKATAVVAYLALNRSVSTERLEEACWYGSDRT
                     SRRKRLRDVLTEARSAIGSQHIPANTNGSYTAGPRLRTDTELFDWHVNAAAAAASAEA
                     VLHYRAALDLVSGIPFSYPGGAGQSFGWIDLEHLATTWEYRIANIARACAEMYLDAGE
                     PGPAIGVLRSVVHALPLDSAVAEALMRAHLANGDRAGLELAYKEHVAALAAADLGDPN
                     DSIEELRADSM"
BASE COUNT          373 a          801 c          993 g          466 t
ORIGIN      
        1 cgccttccac gaaccggtcc gccgactcct agcgctcggc gcggcgaacg gcacgctccg
       61 tccggtcgac cccgagatcg ccgcgacggc cctctacggg gcagtgacgg tcatcggtct
      121 ccgaagcctc gtcatcgaca accacctgga tgtcgacgag gtcagtgggc agatcctgcc
      181 gctgttctgg agcgggatcc ggcccaacac gaggtcctag ctgcgacagt ggtcactagc
      241 ccggcgcccc ctcccactgc ccggcgaaga aggctggcga agaaggacag tatccacgca
      301 gaccgactcg accgtctccc gaggcgccaa ggccgttcgg ggacgcccgc atccatctcg
      361 ccgggtcggc ccgaattaca tgctgtcagc tcggagctcc tcgatcgagt cgtttgggtc
      421 gccgaggtca gccgccgcga gagcagcgac atgctccttg taggcgagct cgagtccggc
      481 acggtcgccg ttggcgaggt gggctcgcat caacgcttcg gcgaccgcgc tgtcgagtgg
      541 gagtgcgtgc acgacgcttc ggagtactcc gatggccggg ccgggttcgc ctgcgtcgag
      601 gtacatctcg gcgcacgctc gagcgatgtt ggcgatgcgg tactcccagg tcgtcgcgag
      661 gtgctcgagg tcgatccaac cgaatgactg tccagccccg ccggggtacg agaacgggat
      721 gcccgacacg aggtcgagtg cggctcggta gtgaagcacc gcctctgcgc tggcggctgc
      781 cgctgctgcg ttgacgtgcc agtcgaacag ctcggtgtcg gtgcgcaagc gtggtccggc
      841 ggtgtagctg ccgttggtgt tcgcggggat gtgttgcgag ccgatcgcgc tgcgggcctc
      901 ggtgaggacg tcgcggagcc gcttgcggcg cgatgtgcgg tcggagccgt accagcaggc
      961 ctcttcaagg cgttcggtcg acacggagcg gttgagcgcc aggtaggcga cgaccgcggt
     1021 cgccttcggc cgcagctgtg ctgcgccacc gtcgacggtg atgtcaccga gaagtcgaac
     1081 caggacgtcg tagccgggcg gctcgttgac cggccacggc tcgtcggggt cgcctccgct
     1141 cgtcgggtcg acgggtacag agacgttgac tgggaccact gcagccggtt catcgaggga
     1201 gtcctcgtcg gctggttgct cggcggccgc aagcagcgca gccatctgtg cgagttcgtc
     1261 gctgtccaag cgttgaggag tgcaggcgag gttcaggtgg tcgaggtaga gcgcatccgg
     1321 ttcgcatcga acgcttgcca cgtctgcgag ttcgccgacg accaccaccg caacagctgc
     1381 cggagagtgt gcccgcagca tctcggccag ctcggtgggt ggcggatggt cggcaacgac
     1441 cacgatcgga ctcaacgcgt cgtggtcggg ctcatgcgcc ctggcaacga acggggtttg
     1501 ccacccgttc tcgacgagtg ataggtggga ttgactcgcc cacgcagcga ggtcgtcggt
     1561 gaggtcgtcc catgagtcgg cgacggtgag gtgctcgagc accgcgacgt cgttctcgac
     1621 gagatccccg atggcgacga cacggatggt gtcggcgagc gggctgaggg tcagttcggt
     1681 gagtatcgac cgggcaagtt ccgtcgcaac gtcatggtcg ccgatcagtg caaggagccc
     1741 gtcggcttcg aggtcgaggt agagctgggc gtcgtcctca ggtgcgccga tggtgaccaa
     1801 gagcggcgct gcgctgggca cgtcgtcgtt cgcgacggta tcgaaggcct cgatcccagc
     1861 ggttccggtg aaggtccaca cggtgccgtc gatggtggtg tcccagccgg acggcgggtt
     1921 ggtgtcgggt tggtcgagga tgacctcggt cgagccggcg gcgtggcgga ccacacttgg
     1981 ccgccgcggc gatgctgtcg ctgcgagacc ggcggcgagg gacccgatga cctgctggag
     2041 ctcgtcgatg cggtcctcgt ccgcgtgagc gatgacctct cggtgcagct cgcgctggcc
     2101 tacgggggtg acagctgaga tctggccagg gtgttcgttc gcgaagcggc ggcgccttcg
     2161 ggcgaggagt cgttggagcc cgacggcgag caccacgctc gacagtgctc cggcggcgac
     2221 ggggaccagg gtgccgcccg ttgccttgtc gcgtggttcg gtgtccgctt ccacgccacc
     2281 ggtggatggg gcgggcgtgg atggggcttc cgtggtcggg gccgctgtcg tggctggtgc
     2341 tgaagtggcc gtgctcgtcg acgccgtgac gtcgcctgga gcagcggtgt cgtcgagcac
     2401 ctccggtgct gcgacaggtg gttcgtcttc ggctggcgcc gggagcggtg cgggtgctgg
     2461 gggtggagct gtctgctcgg cactcggcgg accgagcgag gcgagtccgg tggggggcag
     2521 cacgaggacc tgtccgaggt ggatcaggtt ggggttgcca ggttggacgt agcggtcctg
     2581 gttcgcgtcg atcacttcga gccagtaagg gccgatttcg ggttcggtgg cgt
//
LOCUS       DAOOMA010000390         2623 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_48215_length_2623_cov_2.748053, whole genome shotgun sequence.
ACCESSION   DAOOMA010000390 DAOOMA010000000
VERSION     DAOOMA010000390.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 2623)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2623)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2623
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48215_length_2623_cov_2.748053"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..456)
                     /locus_tag="PLP95_10110"
     CDS             complement(<1..456)
                     /locus_tag="PLP95_10110"
                     /inference="COORDINATES: protein motif:HMM:NF013593.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsH"
                     /protein_id="HPB46194.1"
                     /translation="MGAKRESMVLSDKEKELTAYHEAGHAVCAAVLDHTDPLHKVTII
                     PMGMALGVTISLPEEDRHSYDQKYLEDSIVMAMGGRIAERLVFGVVSTGANNDLVVST
                     ERARKMVREWGMSDRIGPMAWGSQGQVFLGDDLMGGGREYSDETARVIDE"
     gene            complement(440..1705)
                     /locus_tag="PLP95_10115"
     CDS             complement(440..1705)
                     /locus_tag="PLP95_10115"
                     /inference="COORDINATES: protein motif:HMM:NF012234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent metallopeptidase FtsH/Yme1/Tma
                     family protein"
                     /protein_id="HPB46195.1"
                     /translation="MAAEPSPPSQKPNSPQGPFSKGDQGLPRWTLFIVAGIVMIAMFA
                     ATRVESSSRNEIEYSEFLAKVESGDVKEATWDNSTTIITGTLDDGTKFQTTGPVTPDE
                     ATMALLRSNTKLTFHTNQPNLLTDLIPVFLPILLLIGFFVWMQRRAQGQMGGIMSIGR
                     SRAKTYSSERPATTFADVAGYEGVKTEIKEVVDFLKEPDRFSEIGARIPKGILLVGPP
                     GTGKTLIARAVAGEAGVPFLSVSGSDFMEMFVGVGASRVRDLFDSARKMGRAIIFIDE
                     IDSIGRKRGAGLGGGHDEREQTLNQMLSEMDGFEATEGIVMMAATNRPDILDPALLRP
                     GRFDRQVVVPLPEITDRRQILDVHVRHKRLANDVDLDLVARGTPGMSGAELANLVNEA
                     ALHAVRQGSVHPQHRFRVRPRPGAHGRQA"
     gene            1839..2072
                     /locus_tag="PLP95_10120"
     CDS             1839..2072
                     /locus_tag="PLP95_10120"
                     /inference="COORDINATES: protein motif:HMM:NF023430.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3499 family protein"
                     /protein_id="HPB46196.1"
                     /translation="MSRTCARPGCDRHAVATLSYNYANSVVFLEDLAPQAHPMVHDLC
                     GPHADTLRVPRGWTLQDSRRVTSLPMFEHRKTA"
     gene            2075..>2623
                     /locus_tag="PLP95_10125"
     CDS             2075..>2623
                     /locus_tag="PLP95_10125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB46197.1"
                     /translation="MKRPSFVEDVAPPWSSAQAVGVVLGSHASLILGGSLVIVASGYS
                     ASGSVSDIPIGWQAAATSPLWIAAVVLTWFLMRRTHADPVAELGLRFRPVDLVIGVGG
                     GMFIQWVVLPLLYWPIFRLTDTGADELGEAARELADTASGSRWGALVFLMMTVLCAPI
                     AEETIYRGVLLRGLHSFGTWGAI"
BASE COUNT          412 a          821 c          885 g          505 t
ORIGIN      
        1 ttcgtcgatg acgcgagcgg tctcatcgga gtactcacgg ccgccaccca tgaggtcgtc
       61 tccgaggaac acctgccctt gagagcccca cgccatcggt ccgatacggt cgctcatgcc
      121 ccattcgcgc accatcttgc gggcgcgttc ggtggacacg acgaggtcgt tgttggcccc
      181 ggtcgagacg acgccgaaca ccagacgctc ggcgatccgg ccgcccatcg ccatcacgat
      241 cgagtcctca aggtacttct ggtcgtagct gtggcggtct tcctcgggaa gcgagatcgt
      301 gactccgagc gccatcccca tcggaatgat ggtcaccttg tgcaacggat cggtgtggtc
      361 gagcaccgcg gcacacaccg cgtggccggc ctcgtgatag gcggtcagtt ccttttcctt
      421 gtccgacagc accatcgact cacgcttggc gcccatgagc acccggtcgc gggcgtactc
      481 gaaatcgatg ttgcggatgt actgaaccct ggcgaacggc gtgcagggcg gcctcgttga
      541 cgaggttggc cagctcggcg ccgctcatgc ccggcgtgcc gcgggcgacc aggtcgagat
      601 cgacatcgtt cgccaaccgc ttgtgacgca cgtggacgtc gaggatctgg cggcgatcgg
      661 tgatttcggg cagcggaacc acgacctggc ggtcgaagcg gccggggcgc agcagcgcgg
      721 ggtccaagat gtcggggcgg ttcgtggccg ccatcatcac gatgccttcg gtcgcctcga
      781 acccgtccat ctccgacagc atctggttca gggtctgctc gcgctcgtcg tggccgcccc
      841 cgagtccggc gccgcgtttg cggccgatgg agtcgatctc atcgatgaag atgatcgctc
      901 ggcccatctt gcgggccgag tcgaacagat cacgcacccg gctggcgccc accccgacga
      961 acatctccat gaagtccgaa cccgacacgg acaaaaacgg cactcccgcc tcgccggcga
     1021 ccgcacgtgc gatgagggtc ttgccggttc cgggagggcc gacgagcagg atgcccttgg
     1081 ggatgcgagc accgatctcg gagaagcgat cgggttcctt gaggaagtcg acgacctctt
     1141 tgatctcggt cttgaccccc tcgtatccgg cgacatcggc aaaagtggtc gccggtcgtt
     1201 cggaactgta ggtcttggcg cggctgcgcc cgatcgacat gatgccgccc atctgcccct
     1261 gagcccgacg ctgcatccag acgaagaagc cgatcagcag gagaatcggg aggaacaccg
     1321 gtatcaggtc ggtgagcaga ttgggctggt tcgtatggaa cgtcagcttc gtgttgctgc
     1381 gcaacagcgc catcgtcgct tcatctggtg tgaccgggcc ggtggtctgg aacttcgtgc
     1441 cgtcgtcgag cgtgccggtg atgatcgtcg tggagttgtc ccacgtcgcc tctttgacgt
     1501 cgccagactc gaccttcgcg aggaactcgc tgtattcgat ctcgtttcgc gacgaggatt
     1561 cgactcgggt cgcggcgaac atggcgatca tcacgatgcc ggcgacgatg aacagcgtcc
     1621 agcgcggcaa cccctgatcg cccttggaga agggcccttg gggactgttc ggcttctggg
     1681 atgggggaga gggctcggca gccatggcgt acagcctaga agctgtggcg ggccttgcgt
     1741 gatcggaatc tgagaggtcg cgctcagtcc caacctcgca gtcccaagcg ggtggccatc
     1801 cgcgggttgc cgacccgatg acactatcgt cgccgccgat gagcaggacc tgtgcacgtc
     1861 cggggtgtga ccgccacgcc gtcgcgacac tcagttacaa ctacgccaac agcgttgtgt
     1921 tcctcgagga ccttgcgccg caggcccacc cgatggtgca cgacctgtgc gggccccacg
     1981 ccgacacgct gcgggtgccg cgtggctgga cgctgcagga ttcgcgtcgg gtaacgtcgc
     2041 tgcccatgtt cgagcatcgc aagaccgcct gagcgtgaag cgaccctcct tcgtcgagga
     2101 cgtcgcgccg ccgtggtcga gcgcccaggc ggtcggcgtg gtgcttggct cgcacgcgtc
     2161 gttgattctc ggggggagtc tggtgatcgt ggcgtcggga tattcggcgt ccggaagcgt
     2221 ttccgacatt ccgatcggtt ggcaggccgc ggcaacctcg ccgctgtgga tcgcggcggt
     2281 ggtgttgacc tggtttctga tgcgacgcac gcacgccgat ccggtcgccg agttggggtt
     2341 gcggtttcga ccggtcgatc tggtgatcgg ggtcggcggt ggcatgttca tccagtgggt
     2401 ggtgttgccg ttgctgtact ggcccatctt ccgcctgacc gataccgggg ccgacgaact
     2461 gggtgaagcg gcgcgtgaac tggccgacac tgcgtcgggt tcgcggtggg gtgcgttggt
     2521 gtttctcatg atgacggtcc tgtgcgctcc gatcgcggag gagacgatct accgcggcgt
     2581 cctgttgcga gggctgcact cgttcggaac ctggggagcg atc
//
LOCUS       DAOOMA010000391         2620 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_48271_length_2620_cov_3.428460, whole genome shotgun sequence.
ACCESSION   DAOOMA010000391 DAOOMA010000000
VERSION     DAOOMA010000391.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 2620)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2620)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2620
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48271_length_2620_cov_3.428460"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..929
                     /locus_tag="PLP95_10130"
     CDS             <1..929
                     /locus_tag="PLP95_10130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="excinuclease ABC subunit C"
                     /protein_id="HPB46198.1"
                     /translation="PTEPDDSEVYREWLSTQRGSNVEIRVPQRGDKRSLAETVTQNAD
                     EEFRRHRLRRASDHNSRAKALQELQDYLDLPEAPLRIECFDMSHLQGTDYVGSMVVMS
                     DGLPDKREYRRFKVNTVGGQRIGDSDDFAAMEEVLTRRFTNYLEDRAKPVEERGRFQY
                     PPQLLVVDGGKGQLGVAVRVLESLGLDEEIPVCSLAKQFEEVFVPGRSAPVRVPRQSE
                     ALFLLQRIRDESHRFAITFHRERRGKRMTRSVLDDIAGLGPTRRKRLVSELGGVNAVK
                     AASLDTLKALSWLPDAVAEAVYAKTHPEAGDT"
     gene            941..1801
                     /locus_tag="PLP95_10135"
     CDS             941..1801
                     /locus_tag="PLP95_10135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020376889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HPB46199.1"
                     /translation="MSGERTDHSPSHNTSDRGEAGSGASISGAADRGPSVHEANTAAE
                     WERHAAWWQDGFTDGADSEYVEQIIPLVAQWLPAAATVLDIGAGDGQIARMAAANGHV
                     SIAVERSESQIAVGCERDAANAGVAAVRWVRGDAVRLPLADDSVDAAVLCLVIEHIED
                     FEAAFAEVARVLRPGGRMLVLMNHPILQTPNSGWIDDHILEEQYWRIGPYLVETTTSE
                     EVGRDVSLTFHHRPLGRYLNAAADLGLNLTAFIEPAPPSGFLAETPEYSDADTIPRLL
                     LTVHTLAGGR"
     gene            1893..>2620
                     /gene="rapZ"
                     /locus_tag="PLP95_10140"
     CDS             1893..>2620
                     /gene="rapZ"
                     /locus_tag="PLP95_10140"
                     /inference="COORDINATES: protein motif:HMM:NF015620.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HPB46200.1"
                     /translation="MSEILIITGMSGAGRSQFGNHLEDLGWFVIDNLPPALIEQVHEL
                     AIGKRQDVRLAIAMGAGAAEELEAGIAHLRANGSSVRMVFLDATTEVLVRRYESTKRR
                     HPHLVGESLSEAIERERRTLATVRAAADLHIDTSSLTPYALKERVNELFGGDDALDPM
                     ATTVMSFGYKHGVPADVDIVMDCRFLANPFWIDHLRPLTGQDPEIVEYLEAQENTAEF
                     LDRFVDLLELLLPAYRAEGKSYLS"
BASE COUNT          447 a          833 c          920 g          420 t
ORIGIN      
        1 tgccgaccga acccgacgac tcggaggtct atcgcgagtg gctgagcacc cagcggggat
       61 cgaacgtcga gattcgggtg ccgcagcgag gcgacaagcg gtcgttggcc gagacggtga
      121 cacagaacgc ggatgaggag tttcgccgtc atcgtctgcg tcgggcctcc gatcacaaca
      181 gtcgcgccaa ggccctccag gaactgcagg actacctcga tctgccggag gcaccgctta
      241 ggatcgagtg cttcgacatg agtcacctcc agggcaccga ttacgtcggg tcgatggtcg
      301 tgatgagcga cgggctgccc gacaagcgcg agtaccgccg gttcaaggtc aacaccgtcg
      361 gcggccagcg catcggcgac tccgacgact tcgccgccat ggaagaggtg ctgacgcggc
      421 gcttcacgaa ctatctggag gaccgcgcca agcccgtcga ggagcgtgga aggttccagt
      481 acccgccgca actcctggtg gtcgatggcg gcaaggggca actcggcgtg gcggttcgcg
      541 tgctcgagtc gctcggcctc gacgaggaga ttccggtgtg ttcgttggcg aagcagttcg
      601 aggaggtgtt cgtgcccggc cgctcggctc cggtccgggt gccccgccaa tccgaggcgc
      661 tgttcttgtt gcagcggatt cgcgacgaga gccaccgctt tgccatcacg ttccaccgcg
      721 agcggcgcgg caagcgcatg acccggtcgg tgctcgacga catcgccggg ttggggccga
      781 cccgccgcaa gcggctggtg tcggagttgg gtggggtaaa cgcggtgaag gccgcgtcgc
      841 tcgacacctt gaaggcgttg agttggctgc ccgatgcggt ggcggaggcg gtgtacgcca
      901 agacacaccc cgaggccggc gacacatagg ctcgaccccc gtgtcaggcg aacgaaccga
      961 tcacagcccg tctcacaaca cgtccgaccg cggtgaggcc ggcagcggcg cgagcatcag
     1021 cggtgcggcc gaccggggcc cgagcgtgca cgaggccaac accgccgccg aatgggaacg
     1081 ccatgcggcc tggtggcagg acggattcac cgacggggcc gactcggagt acgtcgaaca
     1141 gatcatcccc ctggtggcgc agtggttgcc ggcggcggca acggtgctcg acatcggggc
     1201 cggcgacggc cagatcgcgc gcatggcggc ggcgaacggg catgtctcca tcgccgtgga
     1261 gcgcagcgag tcgcagatcg cggtcggctg cgagcgcgac gccgcgaacg ccggggtcgc
     1321 agccgttcgt tgggtgcgcg gcgacgcggt ccgccttccc ctcgccgacg actcggtcga
     1381 tgccgcggtg ctgtgtctgg tgatcgaaca catcgaggac ttcgaagcgg cgttcgccga
     1441 ggtcgcccgc gtgctgcggc ccggcggacg gatgctggtg ctgatgaatc acccgatcct
     1501 gcagaccccg aattcggggt ggatcgacga tcacatcctt gaggagcagt actggcgcat
     1561 cggcccgtac ctggtggaaa cgacgacgag tgaggaggtg ggccgcgacg tgtcgttgac
     1621 cttccatcat cgtccgctcg gtcggtacct gaacgcggcg gccgacctcg ggctgaacct
     1681 cacggccttc atcgagcccg ctccgccctc cgggttcctg gccgaaaccc ccgagtacag
     1741 cgacgccgac accatcccgc ggctgttgct caccgtccac accctggccg gcggtaggta
     1801 gggctgcgtt tcggcccgaa caggtcggag cgaggggcgc gtcgtatcgg ccagagcgcg
     1861 gatgctgagt ggaacaccga tacggtgaga acgtgagcga aatcctcatc atcaccggta
     1921 tgtccggggc cggtcggtcc cagttcggca atcacctcga ggatctcggg tggttcgtca
     1981 tcgacaacct gccacctgcg ctcatcgaac aggttcatga gctcgcgatc ggcaaacgcc
     2041 aggacgtgcg tttggcgatc gccatggggg ccggtgccgc tgaggaactg gaggcgggga
     2101 tcgcccatct gcgcgcgaac gggtcgagcg tgcggatggt gtttctcgat gcgaccaccg
     2161 aggtgctggt ccgccgctat gagagcacca agcgccgtca tccccatctg gtgggggaga
     2221 gcctgagcga ggcgatcgag cgcgagcgta ggaccctcgc cacggtgcgg gcggccgccg
     2281 acctgcacat cgacaccagc agcctgacgc cgtatgcgct gaaggagcgg gtcaacgagt
     2341 tgttcggtgg cgacgacgcg ctcgacccga tggcgacgac ggtgatgagc ttcggctaca
     2401 agcacggggt gccggccgac gtcgacatcg tcatggactg ccggttcctg gccaacccgt
     2461 tctggatcga tcatctgcgc ccgctgaccg gccaggaccc ggaaatcgtc gagtacctcg
     2521 aagctcagga gaacaccgcg gagtttctcg atcggttcgt cgacctgctg gaattgctgc
     2581 tgccggccta tcgagccgag ggcaagtcgt atctgtcgat
//
LOCUS       DAOOMA010000392         2615 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_48384_length_2615_cov_3.825391, whole genome shotgun sequence.
ACCESSION   DAOOMA010000392 DAOOMA010000000
VERSION     DAOOMA010000392.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 2615)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2615)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2615
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48384_length_2615_cov_3.825391"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..700
                     /locus_tag="PLP95_10145"
     CDS             <1..700
                     /locus_tag="PLP95_10145"
                     /inference="COORDINATES: protein motif:HMM:NF013706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HPB46201.1"
                     /translation="PVDPRTLGCAVGVPRQVFAIGLNYAEHIAEFTSPLGEMPPFFTK
                     FPSCLAGPFDDVPLAGEFMDWEVELVAVISKPARNVSEADALDHVAGYTVGQDITDRV
                     LQQAASGQFCLGKSRRGCGPIGPWVVTPDEFASAELDPTSLKLGCSVNGETMQSGNTE
                     AMIYTVARLVSLLSSVTDLLTGDVIFTGTPSGVGKAQIPPRSLQPGDVLESWIEGIGR
                     LRNRCVAAPTASMG"
     gene            756..2099
                     /locus_tag="PLP95_10150"
     CDS             756..2099
                     /locus_tag="PLP95_10150"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004333 - fumarate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0006106 - fumarate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006108 - malate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HPB46202.1"
                     /translation="MSESTTSAQILYGAETTKAVHNFAISGQPVPMSVVHWLARIKGA
                     AATVNARLGKLDVGVAAAITAAADEVVAGRYDEQFPVDVFQTGSGTSTNMNVNEVISA
                     LTGGVAHPNDHVNMGQSSNDTFPSAVHLAALDAVQRRLLPALDHLASALEAKSVEFHD
                     VVKAGRTHLMDAVPITLGQEFGGYGAQVRLGIERISATLDRVGQIPLGGTAVGNGLNT
                     PAEFASMVRGVLVDLTGLRHVGAPLDHFEAQANRDALVELSGALRGLAVSLTKIAQDI
                     ALMGSGPRAGIGELVLPSLQKGSSIMPGKVNPVMAEVVLQVAAQVIGNDTAITIGGMQ
                     GQFELNVRVPLIARNLLESIDLLTNSTTAFADKCIAGLAVNPAGTERGAHGTLAVATA
                     LNEHIGYDLATKIVAAAADSGRPLREVAIEAGVDADLYDSVIDLRRIAAGNEPLA"
     gene            2203..>2615
                     /locus_tag="PLP95_10155"
     CDS             2203..>2615
                     /locus_tag="PLP95_10155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014578420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HPB46203.1"
                     /translation="MTTPTPEPAPTSDYLQRILTARVYDLAVESPLEFAPSLSERIGA
                     DVWLKREDTQSVHSFKVRGAYNKMVRLTPEQLASGVICASAGNHAQGVALSARHLGCR
                     AVVVMPRTTPQVKLDAVAALGAEVVLHGDNYSDAA"
BASE COUNT          443 a          865 c          863 g          444 t
ORIGIN      
        1 gccggtcgat ccacgcaccc tcggttgcgc ggtcggggtg ccccgtcagg tgtttgccat
       61 cgggctgaac tacgcggaac acattgccga gttcacgtcg ccgctgggtg agatgccgcc
      121 atttttcacc aagtttccgt cctgtctggc gggaccgttc gacgacgttc cactagctgg
      181 ggagttcatg gattgggagg ttgaactcgt cgcggtcatc tccaaaccgg cgcgcaacgt
      241 gtcggaggcg gacgcgctcg atcacgtcgc cgggtacacg gtcggccagg acatcaccga
      301 ccgggtgttg cagcaggcgg cgagcgggca gttctgtctc ggcaagtcgc gtcggggctg
      361 tggcccgatc gggccgtggg tggtgacccc cgacgagttc gccagtgccg agctcgatcc
      421 gacctcgttg aagctgggtt gttcggtaaa cggggagacg atgcagtcgg gcaacaccga
      481 ggcgatgatc tacaccgtcg cccgactggt ttcgttgttg tcgtcggtga ccgatctgct
      541 caccggcgat gtgatcttca ccggcacacc atcgggtgtc ggcaaggccc agattccgcc
      601 gcgctcgctt cagcccggag acgtgctcga atcgtggatt gaaggcattg gtcgcctgcg
      661 caaccgttgc gtcgcagcac cgacggcgtc aatgggatga cggtgggtta gccggaacaa
      721 ccgagtagcc ggagcaacag atcaggagcg cggagatgag cgaatcaacc acgtcggcac
      781 agatcctgta cggggccgag accaccaagg ccgtgcacaa ctttgcgatc tccgggcagc
      841 ccgttccgat gtcggtcgtg cactggctcg cccgcatcaa gggggccgct gccacggtga
      901 atgcccggct cggcaagctc gacgtcggcg tcgccgcagc gatcaccgcc gcggccgatg
      961 aagtcgtcgc cggacgctac gacgagcagt tccccgtcga cgtgttccag accggttcgg
     1021 gaacctccac caacatgaac gtcaacgagg tcatctcggc gctcaccggt ggcgtcgctc
     1081 acccgaacga ccacgtgaac atgggccaat cctccaatga cacgttcccc tcggccgttc
     1141 acctcgccgc cctcgatgcc gtgcagcgac gcctgttgcc ggcgctcgac cacctcgcat
     1201 cggcgctcga ggcgaagtcc gtcgagttcc acgacgtcgt caaggccggg cggacccacc
     1261 tgatggacgc ggtgccgatc accctcgggc aggaattcgg tggatatggc gcccaggtcc
     1321 gcctcggcat cgagcgaatc tcggcaacac tagatcgggt cgggcagatc ccgctcggcg
     1381 gaacggcggt cggcaacggg ctcaacactc cggcggagtt tgcctcgatg gtgcgcggcg
     1441 tgctggtgga cctcaccggg ttgcgccacg tcggcgcgcc gctcgatcac ttcgaggccc
     1501 aggcgaatcg cgatgcgctg gtcgagctgt ccggtgcgct tcgtggcctg gcggtgtcgc
     1561 tgacgaagat cgcccaggac atcgcactga tgggttcggg gcctcgagcg ggcatcggcg
     1621 aactggtgct gccctcgctg cagaagggtt cctcgatcat gcccggcaag gtcaacccgg
     1681 tgatggccga ggtggtgttg caggtcgcgg cccaggtcat cggcaacgac accgccatca
     1741 cgatcggggg catgcaaggg cagttcgaac tcaacgtgcg ggtgccgctc atcgcccgga
     1801 acctgctcga gtcgatcgac ctgctcacta actcgacgac ggccttcgcc gacaagtgca
     1861 tcgctgggct ggcggtcaat cccgccggta ccgaacgcgg cgcccacgga accctggcgg
     1921 tggccaccgc gctgaacgaa cacatcggct atgacctggc gacgaagatc gtcgcggctg
     1981 cggccgacag cgggcgcccg ttgcgcgagg tggcgatcga ggccggggtc gacgccgacc
     2041 tgtatgactc ggtcatcgac ctgcgccgca tcgccgcggg caacgaaccg ctcgcctgaa
     2101 ccgctcgcct gacccacccc agcctgttct ccgataacca gtggtcgtga tcacgaccac
     2161 tggttagcgg agaacgagct caggcgggtc atactttcct gcatgaccac gccgaccccg
     2221 gaaccggcac cgacctcgga ttacctgcag cggattctca cggctcgggt ctatgacctc
     2281 gcggtcgaat cgccgctgga gttcgctcca tcgctgtccg agcgcatcgg tgcggacgtg
     2341 tggctcaaac gcgaggacac ccaaagcgtg cacagcttca aggtgcgcgg tgcttacaac
     2401 aagatggtcc ggctcacccc cgaacagctc gccagcggcg tgatctgcgc gtcggctggc
     2461 aaccacgccc agggggtggc gttgagcgcc cgtcacctcg gctgccgagc ggtcgtggtg
     2521 atgccacgca cgaccccgca ggtgaaactc gacgccgtcg cggcgctcgg tgcggaggtc
     2581 gtgttgcacg gcgacaacta ttccgatgcc gccga
//
LOCUS       DAOOMA010000393         2615 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_48385_length_2615_cov_3.745312, whole genome shotgun sequence.
ACCESSION   DAOOMA010000393 DAOOMA010000000
VERSION     DAOOMA010000393.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 2615)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2615)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2615
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48385_length_2615_cov_3.745312"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            52..198
                     /locus_tag="PLP95_10160"
     CDS             52..198
                     /locus_tag="PLP95_10160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB46204.1"
                     /translation="MDTGTKLATMGTASVIQFQQHLQPGWMASTVDTASDAIPTEERA
                     APNE"
     gene            213..1445
                     /locus_tag="PLP95_10165"
     CDS             213..1445
                     /locus_tag="PLP95_10165"
                     /inference="COORDINATES: protein motif:HMM:NF012296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HPB46205.1"
                     /translation="MTDFKPSERDDGLIDWITDFDILDESYVEEPAAVWRSLRAACPV
                     AHTDRYGSTWLPVDYDDLASIAHDVERFSSRDVAAITPGRDSDPEAVMMLEAPPITSD
                     PPVHASARRLLLPRFGPSAVGELTPITAGIADALIDDFLDAGAADSAAQYAQHIPVQV
                     ISRMIGLPLEDETMFTEWVVDMLQGGFQNIEKASESVLAVIRYFEGKLEERVEVPPAE
                     RPDDLLTLLIEAEHEGAPLTDRHRIGSCFLLLVAGIDTTWSSIGSALWHLATHPEDQA
                     RLRAEPELMDCAIEEFLRFYSPVTMARYVVDDTEFGGCPMKRGEKVLMAFPAGNRDPA
                     HFDRADEFVIDRQLNRHFAFGSGIHRCLGSNLARMELRVAIERFLARVPTFELTDPGA
                     VSWTGGQVRGPRAVPVAW"
     gene            complement(1608..2099)
                     /locus_tag="PLP95_10170"
     CDS             complement(1608..2099)
                     /locus_tag="PLP95_10170"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HPB46206.1"
                     /translation="MAASPGFVDAIKNRRLLGEVTDGAFIDMFSDSPVSGSANPLSIG
                     LLIGREGDQAVGRVTLERGWEGAPGRSHGGIVAACIDETIGGLLPVLGEMAFTGELAI
                     RYQAPCPMGIPLEFRAWLEHRDGRKLHVSCTGTADGVVFARATALFIVVDLERFRRYS
                     GTD"
BASE COUNT          467 a          856 c          842 g          450 t
ORIGIN      
        1 ggctgcggct gaaacgccgt ggctgacgag acgtacgacg tacactgtct cgtggataca
       61 ggaacgaagc tcgccacgat ggggacggcc tcggtgatcc agttccagca acatctacaa
      121 cccggttgga tggcgtcgac cgtcgacacc gcgagcgacg caataccgac cgaagaacgt
      181 gcagcgccca acgaatgaga aagggacacg cgatgaccga cttcaagccg tccgagcgcg
      241 atgacggtct gatcgactgg atcaccgact tcgacatcct cgatgagtcc tatgtcgagg
      301 aacccgccgc agtctggcgc tcgctccgag ccgcttgtcc ggtcgcgcat acggatcggt
      361 acgggtcgac gtggctgcca gtcgactacg acgatctggc gtcgatcgct cacgacgtcg
      421 agcgcttctc gtcgcgcgat gtcgccgcca tcactccggg acgcgactcc gaccccgagg
      481 ccgtgatgat gctggaggcg ccgcccatca cgtccgaccc accggtgcac gcatcggcga
      541 ggcggctcct gctgccgagg ttcgggccgt ccgccgtcgg cgaactcacg ccgatcacgg
      601 caggaatcgc tgacgcgctg atcgatgact tcctcgatgc cggcgcagcg gactccgccg
      661 cccagtacgc gcagcacatc cccgtgcagg tcatctcccg catgatcggg ctgccgctcg
      721 aagacgagac gatgttcacg gagtgggtgg tcgacatgct ccagggggga ttccagaaca
      781 tcgagaaggc gagcgagtcg gtgctcgcgg tgatccgcta cttcgaggga aagttggagg
      841 aacgggtcga ggtacccccc gctgagcgac ccgatgacct gctcaccctt ctcatcgagg
      901 cggagcatga aggagcaccg ctcaccgacc ggcaccgcat cggcagctgc ttcttgctgt
      961 tggtcgctgg catcgacacc acgtggagct cgatcgggtc ggcgctctgg catcttgcga
     1021 cgcaccccga ggatcaggcg cgtcttcgcg ctgaacccga gctcatggac tgcgcgatcg
     1081 aggagttcct ccgcttctac agcccggtca ctatggccag atacgtcgtc gacgacaccg
     1141 agttcggagg ctgtccgatg aagcgcggag agaaggtcct gatggcattc cctgcgggca
     1201 atcgggatcc agcacacttc gaccgcgccg acgagttcgt catcgaccgg cagctcaacc
     1261 gccatttcgc gttcggttcg ggtatacacc gctgcctcgg ttcgaatctc gcccggatgg
     1321 aactgagggt cgcgatcgaa cggttcctcg cccgcgtgcc gaccttcgag ctgaccgacc
     1381 cgggagcggt gtcgtggact ggtggacagg ttcgtggtcc tcgggccgtc ccagtggcct
     1441 ggtgaggatc cccgtgagtg cgcgagtcgc ggcctgcgta gagttgtcgt agtcgagact
     1501 gccgatcgcc tgatcccggc cgactcgatg gccgatgatc gttgctccac cgtcgaagcg
     1561 atcgattgct acctccccag gccggagtgc tctccgactg acggagctca gtcggtaccg
     1621 gagtagcgcc ggaaccgttc gagatcgacg acgatgaaca gggccgtcgc tcgtgcgaag
     1681 acaactccgt ccgctgtccc tgtgcacgag acatgcagct tccgcccgtc gcgatgctcc
     1741 agccacgcgc ggaactcgag cggaatgccc atcggacagg gcgcctggta ccggattgcg
     1801 agttccccgg taaaggccat ctcgccgagc acagggagga gcccgccgat cgtctcgtcg
     1861 atgcacgcag cgacgatgcc tccatggctg cggcctggtg ccccctccca gccacgctcg
     1921 agtgtcaccc gaccgacagc ctggtcgccc tcgcggccga tgaggagacc gatgctgagc
     1981 ggattggcgg agcccgagac cggagagtca ctgaacatgt cgatgaacgc cccgtcggtg
     2041 acttcgccga gcagccgacg gttcttgatc gcatcgacaa atccagggct cgctgccatc
     2101 tcgtcgccca gagtccggct cggttccagt tctaggcgtt cagcaaggga cgttgccctc
     2161 tgtgcgagcg cttccaagat ctcgggctcc gcccggtgcc ccaccagcgc gtgactgagt
     2221 cggcggagtg acctggccgc agcgacacgg agcgggaggt cgtcgtgggt gtcttcttga
     2281 tcgcctgacg cacgctccat ggcgacgatg atcgcgcgag ctgaaacgaa caacacaact
     2341 cgtcaccagt ctcatgaccg tggaacgggt caccgaaggg cgtaccgagt tcaccaacgc
     2401 cagacatcgg atgatcggct ggaacgatca caccgctcca tccgtcagac agttcgttca
     2461 tgcgagagct tcggcggggc tgtcagcgag tgcgtcgaac tcggcggtga ggcgggcgcc
     2521 atcagggtgg tcttcgagct cggtgcgcac ctgcgcgagc cggtacgcga ggacgcggtc
     2581 gaaccggtcg cctgtctggt tctccaacgc ggcgc
//
LOCUS       DAOOMA010000394         2611 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_48490_length_2611_cov_3.118545, whole genome shotgun sequence.
ACCESSION   DAOOMA010000394 DAOOMA010000000
VERSION     DAOOMA010000394.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 2611)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2611)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2611
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48490_length_2611_cov_3.118545"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..329)
                     /locus_tag="PLP95_10175"
     CDS             complement(<1..329)
                     /locus_tag="PLP95_10175"
                     /inference="COORDINATES: protein motif:HMM:NF013225.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HPB46207.1"
                     /translation="MRRDVNWIGLIADPGSEQPLLGDDESVAAAWIRVEGRRVMFVWS
                     RFEVAAGTLSIAAGKRLVDAFDAAARDETPVFALANSGGARMQEGTRAFMQMVGAARA
                     ARRVRSAG"
     gene            complement(339..1889)
                     /locus_tag="PLP95_10180"
     CDS             complement(339..1889)
                     /locus_tag="PLP95_10180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020378767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase large subunit"
                     /protein_id="HPB46208.1"
                     /translation="MNGARALIQTLVDAGVEVCFTNPGTSEMHFVAALDEVEGMTAML
                     CLFEGGATGAADGYARMADKPAATLLHLGPGLGNGFANLHNARRAGVPMVNIVGDHAT
                     YHKQYDAPLESDMDAVAGACSGAVIRSTTPDQVAGDAADAVAQSLASPNQVVTLALPA
                     DVCWLPAPGPVAPVVPAAPKPVDEALLAELAAALSEPGAVLLLGGRALRAAPRVAGAR
                     VAAATGARVFAETFPAREERGAGLPPLDRIAYLAEFAQMQLAGATRLVAIDAAAPVSF
                     FAYPDKASVLTPDDCVVTVVSREHDPAELLEALAAHLDAPVEVEVAEPSRPDLPTGEL
                     TSESVAAAIGALLPEGAIVVDEANTSGLFIPGATAGAPRHDWLCLPGGAIGFGIPTAT
                     GAAVACPDRPVLSLEADGSAMYTLQALWTQARENLNVTTVILKNSSYAVLNMELSRVG
                     AEAGGEQAKRMLDLTGPELDFVALARGLGVDASRATTAEEFVTALRSGLATDGPTLIE
                     AVVPALGL"
     gene            complement(1951..>2611)
                     /locus_tag="PLP95_10185"
     CDS             complement(1951..>2611)
                     /locus_tag="PLP95_10185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012226318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HPB46209.1"
                     /translation="VIDSGLGPQILEYIDLLTMAAMTASGDLDLGIPEEVKTSALAYL
                     VVVLENSHDDRLEADLHALGMLLSDLGAMDAYVLPAGAATQLIEAREKAFWLAKASGA
                     NDVVDIVVPRATIPEFMEAVQRIAGETATWIAGCGHAGDGNVHLGVFQADPEVRTSVM
                     MQLYRAGMDLGGAISGEHGIGTEKRGYYLELEDPAKLELQRRIKAAFDPNGILNPGAV
                     F"
BASE COUNT          423 a          895 c          906 g          387 t
ORIGIN      
        1 ccggcagagc ggacccgacg ggcggcccgc gctgcaccga ccatctgcat gaatgcccga
       61 gtgccctcct gcatgcgcgc cccgccggag ttggcgagtg cgaacaccgg cgtctcgtcg
      121 cgggcagctg cgtcgaacgc gtcgaccaac cgcttccccg ccgcgatcga cagcgtcccc
      181 gccgcgacct cgaaacggga ccacacgaac atgacgcgtc ggccttcgac gcggatccac
      241 gccgcggcaa ccgactcgtc gtcacccaag agcggctgtt cggatccggg atcggcgatc
      301 aacccgatcc agttcacgtc acggcgcatg ggttcacgct acagaccgag tgccggaacc
      361 accgcttcga tgagggtggg tccgtcggtc gcgaggcccg aacgcaacgc cgtgacgaac
      421 tcctcggcgg tggtcgcacg ggaggcatcc acccccaggc cccgggccag cgccacgaag
      481 tcgagttcgg gtccggtgag gtccaacatg cgcttggcct gctcgccacc ggcctcggct
      541 ccgacccggc tgagctccat gttcagcacc gcgtaggagg agttcttcag gatcaccgtc
      601 gtcacgttga ggttctcgcg ggcctgggtc cacagcgcct ggagggtgta catcgccgat
      661 ccgtcggcct cgaggctcag caccgggcga tccggacagg ccaccgcggc accggttgcc
      721 gtcgggatgc cgaatccgat ggcgccaccc ggcaggcaga gccagtcatg gcgtggagcg
      781 ccagcggtcg cgcccggaat gaacagtccg gaggtgttgg cctcgtcgac gacgatcgct
      841 ccctctggca gcaacgctcc gatggccgca gcgacgcttt cggaggtgag ttccccggtc
      901 ggcaggtcgg gacgcgacgg ctcggccacc tcgacctcga ccggcgcgtc gaggtgcgcg
      961 gcgagcgctt cgagcagttc ggccgggtcg tgttcgcgcg acaccaccgt caccacgcag
     1021 tcgtcggggg tgagcaccga cgccttgtcc gggtaggcga aaaaggacac gggtgcggcg
     1081 gcgtcgatgg cgacgagtcg ggtggcgccg gcgagttgca tctgcgcgaa ctcggcgagg
     1141 taggcaatcc ggtcgagcgg cggcagcccg gccccacgct cctcgcgggc ggggaaggtc
     1201 tcggcaaaca cccgcgcacc ggtcgccgcg gccacacggg caccggccac gcgaggagcg
     1261 gcgcgcaacg cacggccgcc cagcaacaac accgcaccgg gctccgacag cgcagcggcc
     1321 aactcggcga gcaacgcctc gtccaccggc ttgggggccg cggggacgac gggcgccaca
     1381 ggcccggggg ccggaagcca gcacacgtct gccggtaacg cgagggtcac gacctggttc
     1441 ggcgaggcca acgactgcgc caccgcatcg gcggcgtccc cggcgacctg atcgggtgtc
     1501 gtcgagcgaa tcacggcgcc cgaacacgcc ccagccacgg catccatgtc cgattcgagc
     1561 ggggcgtcgt actgcttgtg gtaggtcgcg tggtcgccga cgatgttgac catcggcacc
     1621 ccggcacggc gagcgttgtg gaggttcgca aagccgttgc cgagccccgg gccgagatgc
     1681 aacagggttg cggcgggctt gtcggccata cgtgcatacc cgtcggcagc accggtcgcg
     1741 ccgccctcga acaggcacag catcgccgtc ataccctcga cctcgtcgag tgccgcgacg
     1801 aagtgcatct cggaggtgcc ggggttggtg aaacacacct cgaccccggc gtcgacgagg
     1861 gtctggatga gtgcgcgtgc gccgttcatg tgatgctcct gcggctggaa agactggaag
     1921 gactggaatg gcggctgttg gtgccgtcga ttagaagacg gcgcccgggt tgaggatgcc
     1981 gttgggatcg aatgctgcct tgatgcggcg ttgcaactcg agcttggccg ggtcttcgag
     2041 ttcgaggtag tagccgcgct tttcggtgcc gatgccgtgc tctcccgaaa tggcgccgcc
     2101 gaggtccatg ccggcccggt agagctgcat catcaccgag gtgcggacct cggggtcggc
     2161 ctgaaacacg ccgaggtgca cgttgccgtc acctgcgtgg ccgcacccgg cgatccacgt
     2221 cgcggtctcc cccgcgatgc gctggaccgc ctccatgaac tccgggatcg tcgcacgggg
     2281 caccacgatg tcgaccacgt cgttggcacc actggccttg gcgagccaga acgccttctc
     2341 gcgggcctcg atgagctgcg tcgccgcccc tgcgggcaac acgtatgcgt ccatcgcacc
     2401 gaggtcgctg agcaacatgc cgagcgcatg aaggtcggct tcgaggcggt cgtcatggga
     2461 gttctcgagg acgaccacca gatacgcgag cgccgaggtc ttcacctcct cggggatgcc
     2521 gaggtcgagg tcgcccgatg cggtcatcgc cgccatagtc aacaggtcga tgtattcgag
     2581 gatctgtggc ccgaggcccg aatcgatgac g
//
LOCUS       DAOOMA010000395         2609 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_48538_length_2609_cov_3.836335, whole genome shotgun sequence.
ACCESSION   DAOOMA010000395 DAOOMA010000000
VERSION     DAOOMA010000395.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 2609)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2609)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2609
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48538_length_2609_cov_3.836335"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..286
                     /locus_tag="PLP95_10190"
     CDS             <1..286
                     /locus_tag="PLP95_10190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015439820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HPB46210.1"
                     /translation="ERMAEWIGARNAEEVLAAFDAAEAAAAPVYDMADVAADPHLGER
                     KALTEIDGVTMQGLVARLSATPGQIRHAGRRLAEDQEILDADDPWGSARS"
     gene            complement(460..1353)
                     /locus_tag="PLP95_10195"
     CDS             complement(460..1353)
                     /locus_tag="PLP95_10195"
                     /inference="COORDINATES: protein motif:HMM:NF012596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HPB46211.1"
                     /translation="MSDGTVRLERDESAGIATITLDNPARKNAYDPPMRRQFHAYLEE
                     LAYDDATKVVHLRGADGVFSTGADMNNAYAWYGEGAKSPNDASPEARSKPRRPSQRRR
                     LLVDRQTFDFYHFFLGFPKVTVAEVSGFALGGGFELALMADIAVVASDAELGMPATRM
                     LGPALGSLHMFFHRLGPVLARRMLLTGDTLAGAELAHLGVFTEVCEPDEVAERAKWWA
                     TKASKMPADGIVIAKEAFRLIENLTAYQGEEVLSYMFHAYGTNLQFEPDEFNFVKARS
                     EHGTKAAFTMRDAHFDVPEPS"
     gene            1481..2524
                     /locus_tag="PLP95_10200"
     CDS             1481..2524
                     /locus_tag="PLP95_10200"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HPB46212.1"
                     /translation="MEFRLDEAQVELQQTVARLCRDRVPLDLIAEREGRAVDPQVWAA
                     LAELGVLGLMVPEAEGGVGLGVIEAAIVFEQLGSHPFGGPLVWSVLAAPYLAGVTEGS
                     TIVTGIEASSIVDGSALVEHLDAATVMLVVHGDRVVAHDVASLPPATPLEPLDPLATI
                     SRLDGLDGLDGLAAGDAGGGQVIVEGAAEVEGFVTLAKVLGAALLSGVADSSLHVARD
                     YALEREQFGAAIGSFQAVKHLLADMYGRTVSAQSLTYAAAAVLDDPIEGDTPARDAAS
                     AKLLAADAAITNASTAVQILGGMGFTWDMLPNYLLKRAWALDNEFGTVEHHEALVGRA
                     VGALVSTLAGEVG"
BASE COUNT          398 a          799 c          980 g          432 t
ORIGIN      
        1 cgagcggatg gccgagtgga tcggtgcccg caacgccgag gaggtgctcg ccgcgttcga
       61 cgctgccgag gccgccgcag cacccgtcta cgacatggcc gatgtcgccg ccgatcccca
      121 cctcggcgag cgcaaggcat tgaccgaaat cgatggggtg accatgcagg ggcttgtcgc
      181 acgcctgtcg gccacccccg gacaaatccg acacgccggg cgccgactcg ccgaggatca
      241 ggagatcctc gacgcggacg acccgtgggg ctcggcccgt tcgtgaggcg cggcccgctg
      301 cctggccgct gcccggccgc tgccggaccg ctgccgacgt tctgccggcc gctggcgagg
      361 ttctgtaggt aatcgcggtg gctataggcg ccgcgattac ctacagaacg ggcgagggcg
      421 ggcgagggcg ggcgggtgcg ggcgggtgcg gggctgggct caggagggct cgggaacgtc
      481 gaagtgggcg tcgcgcatgg tgaaggcggc cttggtgccg tgctcggagc gcgccttgac
      541 gaagttgaat tcgtcgggtt cgaactgaag gttcgttccg taggcgtgga acatgtacga
      601 gagcacctcc tcgccctgat acgcggtgag gttctcgatg aggcggaacg cttccttggc
      661 gatgacgatg ccatccgccg gcatcttgga ggccttggtc gcccaccact tcgcccgttc
      721 ggcgacctcg tcgggttcgc agacctcggt gaacacgccg aggtgcgcca actcggcacc
      781 cgccagggtg tcgccggtca acagcattcg ccgggcgagg accgggccga ggcggtggaa
      841 gaacatgtgg agcgacccga gcgccgggcc gagcatgcgg gttgcgggca ttccgagttc
      901 ggcgtcggac gccaccaccg cgatgtcggc catgagcgcc agttcgaacc caccgccgag
      961 cgcgaagcca ctgacctcgg cgacggtgac cttcgggaag ccgaggaaga aatggtagaa
     1021 gtcgaaggtc tggcgatcga cgagcagtcg gcggcgttgg ctggggcgcc ggggcttgga
     1081 gcgtgcctcc ggcgaagcgt cgttcggaga cttggcgcct tcgccgtacc aggcatacgc
     1141 gttgttcatg tcggcaccgg tgctgaacac gccgtccgcg ccgcgcaggt ggacgacctt
     1201 ggtggcgtcg tcgtaggcca actcttcgag atacgcgtgg aattgccgac gcatcggtgg
     1261 gtcatatgcg ttcttgcgcg ccgggttgtc gagggtgatc gtggcaattc ccgccgattc
     1321 gtcgcgttcc agtcgaaccg ttccgtcact catgttgtcg tcccctccgg cgatgcgttg
     1381 tcttcagcgc cgtgtagacc gtaggcttaa cctatagagg tttacaacca ccgatcgtga
     1441 aagcgtcgcg gtggatccga ggggaagggg agggacccga gtggagtttc gactggatga
     1501 agcgcaggtc gagctgcaac agaccgtggc gcgcctgtgc cgcgatcggg ttccgctcga
     1561 cctcattgcg gagcgcgaag ggcgtgcggt cgacccacag gtctgggcgg ccttggccga
     1621 actcggggtg ctcgggctga tggttcccga agccgagggc ggggtgggcc tgggagtcat
     1681 cgaggcggcg atcgtgttcg aacaactcgg ctcgcatccg ttcggcggcc cgctggtgtg
     1741 gagcgtgctc gctgcgccgt atctcgccgg ggtgaccgag gggtcgacca tcgtgacggg
     1801 gatcgaggca agctccatcg tcgacggctc ggcgctggtg gagcacctcg acgccgcgac
     1861 cgtgatgttg gtggtgcacg gggaccgagt cgtcgcgcac gacgtcgcgt cgctgccgcc
     1921 ggcgacaccg ctcgaacccc tcgatccgct ggcgacgatc tcgcggctcg acgggctcga
     1981 cgggctcgac ggtctcgccg cgggagacgc gggaggcggg caggtgatcg tcgagggtgc
     2041 cgccgaggtg gaggggttcg tgaccctggc caaggtgctc ggtgccgcat tgttgagcgg
     2101 cgtggcggat agttcgttgc atgtcgcccg ggactacgcg ctcgaacgcg aacagttcgg
     2161 cgctgcgatc ggttcgttcc aggcggtgaa gcatctgctc gccgacatgt acgggcgcac
     2221 cgtttcggcg cagagcctca cctacgccgc ggctgccgtg ctcgacgacc cgatcgaggg
     2281 cgacacaccg gcacgcgacg ccgcgagcgc gaagctgctg gcggcggacg cggcgatcac
     2341 caacgcgtcg accgcggtgc agatcctcgg cgggatgggg ttcacgtggg acatgttgcc
     2401 gaactacctg ctcaagcggg cgtgggcgct cgacaacgag ttcggcacgg tcgagcacca
     2461 cgaggcgctg gtcggtcggg cggtcggcgc gctcgtcagc acgctcgcgg gggaggtcgg
     2521 gtgagcggac ctagatctgg ggcacacgag gtggtgaggt gagacccgcc tgatggtcca
     2581 gtcggtgtgc gactctgaac agaggagca
//
LOCUS       DAOOMA010000396         2609 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_48541_length_2609_cov_3.215348, whole genome shotgun sequence.
ACCESSION   DAOOMA010000396 DAOOMA010000000
VERSION     DAOOMA010000396.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 2609)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2609)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2609
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48541_length_2609_cov_3.215348"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            73..2085
                     /locus_tag="PLP95_10205"
     CDS             73..2085
                     /locus_tag="PLP95_10205"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPB46213.1"
                     /translation="MSDRAADLDELSPAELAAREAVAADAPAAVAAAGAVAGAADELN
                     RLVSTDRSTLYRDGARLVARIARAHPAPMVISVAAAFLFSGATVLGTVVLGRITDEVI
                     TPAFGDKGVSASTLWAAFAAVVVMSLLRGSGVIIRRYFAAVLEASMQATLRRGVVDKY
                     LTVPLRYYHTKPTGELLSRTDSDVIGTTTMIKALPFSIGVAALVVFALISLAAADWTF
                     ALIAVVMFPLITVLYRIYSGLAVDPIRRSQERIAHVSAVAHESFDGALVVKTLGIEDV
                     ERDRFAAAADELRQERYRLAALRALFEPVLEVLPSVGTIVLVLVGAWRIDSGAASVGN
                     LVQAAAMFSLLAMPMRIFGFFLQELPRSVVSIRRVDEVLDEADEPDGAGVVAPDRDRA
                     VGVRFDNVTFSYGTSTPDEVGEGVGVDAGEGGVEVLDGVTFEIAPGETVALVGSTGSG
                     KSTINDLLIRVIDPDSGTISVDGHDISTIDRQSLSSLVRIAFQESFLFSTTIRDNIVL
                     AAGGGAAGDGEAGDSDLDARLEHVARVARVDRFVERLPAKWDTVVGERGVTLSGGQRQ
                     RVALARALMGSPKVLLLDDATSAVDPTIEAEILANLRAEHATLMVVAHRLSTILLADR
                     VLFLNDGRIAGDGTHADLLANPDYAALVHAYEETETDEVEGEGDDD"
     gene            2078..>2609
                     /locus_tag="PLP95_10210"
     CDS             2078..>2609
                     /locus_tag="PLP95_10210"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HPB46214.1"
                     /translation="MTDARPAGARPADDLPAGAWSVLKRGLAESPELRRGVKSSAAVG
                     ILIAISRLVIPIAVQQIIDRGITGPDGFRPGFVIGCCSAALAALVLVAVGGRFTYYRM
                     ARVAEDTLYGLRTRAFDHIHRLSLSTHTESKRGVLVSRVTSDIDTLTQFAGWGALSWI
                     MNSAVLVGVLLVMFVYS"
BASE COUNT          386 a          907 c          852 g          464 t
ORIGIN      
        1 ccagtggtcg tgatcacgac cactggttat cgggaaacgg gctggggtgg gctcacgtaa
       61 tgtcgagagg cgatgtccga ccgcgccgca gacctcgacg aactgagccc cgctgaactc
      121 gccgcgcgcg aagctgtcgc cgccgacgct cccgcggccg tcgcggcagc gggcgccgtg
      181 gcgggcgcgg ccgacgagct caaccggctc gtcagcaccg atcgcagcac cctgtatcgc
      241 gacggcgccc gactcgtcgc ccggatcgct cgcgctcatc cggccccgat ggtcatctcg
      301 gtcgcagcgg ccttcctgtt ctcgggcgcc accgtgttgg gcaccgtggt gctcggccgt
      361 atcaccgacg aggtcatcac cccggcgttc ggtgacaagg gcgtgagcgc ctcgaccttg
      421 tgggcggcct tcgctgcggt cgtcgtcatg tcgctgctgc gcggttcggg cgtcatcatc
      481 cggcgctact tcgcggcggt gttggaagcc tcgatgcaag cgacactgcg ccgcggcgtc
      541 gtcgacaaat acctcaccgt cccgctgcgc tactaccaca ccaaacccac gggcgaactg
      601 ctcagccgca ccgactccga cgtcatcgga accaccacga tgatcaaggc gttgccattc
      661 agcatcggcg ttgccgcgct cgtcgtgttc gccctcatct ccctcgcggc agcggactgg
      721 accttcgccc tcatcgcggt cgtcatgttc ccgctcatca cggtgttgta ccgcatctac
      781 tcgggcctcg ccgtcgaccc gatccgccgc tcgcaggaac gcatcgccca cgtctcggcc
      841 gtcgcccatg aaagcttcga cggggccctc gtggtcaaga ccctcggaat cgaagacgtc
      901 gaacgagacc gattcgccgc cgctgccgac gagctccgcc aagagcgcta ccgactcgcc
      961 gcgcttcggg cgttgttcga accggtgctc gaggtgctgc cctcggtcgg cacgatcgtg
     1021 ctggtgctcg tgggagcatg gcggatcgat agcggcgcgg cgtcggtcgg caacctcgtg
     1081 caggcggccg cgatgttctc gctgttggcc atgccgatgc gcatcttcgg gttcttcctg
     1141 caggaactgc cacgctcggt ggtgtcgatc cgccgagtcg atgaggtgct cgacgaggcc
     1201 gacgagcccg atggcgccgg ggtggtggcg cccgaccgcg accgcgccgt gggcgtccgg
     1261 ttcgacaacg tcaccttctc ctatgggaca tcgacgccgg acgaggtggg cgagggtgtc
     1321 ggcgtggacg cgggcgaggg cggtgtcgag gtgctggacg gtgtgacctt cgaaatcgca
     1381 ccgggcgaaa ccgtggcgct cgtcggatcg accggatcgg ggaagtcgac catcaacgac
     1441 ctcctcatcc gggtgatcga ccccgattcg ggaacgattt cggtcgacgg ccacgacatc
     1501 tccacgatcg atcgtcagtc gctgagctcg ctggtacgca tcgcgtttca ggagagcttc
     1561 ctgttctcga ccacgattcg ggacaacatc gtgctggccg ccggcggcgg tgctgctggc
     1621 gacggtgaag ccggcgatag tgacctcgac gcccggctcg aacacgtggc gcgcgtcgct
     1681 cgcgtcgacc gattcgtcga gcggctccca gcgaagtggg acacggtcgt gggggagcgc
     1741 ggcgtcacgc tttcgggtgg ccagcgccag cgcgtggccc tggctcgggc attgatgggc
     1801 tcacccaagg tgctcttgct cgacgacgcc acctccgcgg tcgaccccac gatcgaggcc
     1861 gaaatcctcg ccaatctccg cgccgagcac gccacgctca tggtggtcgc ccaccgactg
     1921 tcgaccatct tgttggccga ccgggtcttg ttcctcaatg acgggcgcat cgcaggagac
     1981 ggcacccacg ccgacctcct cgccaacccc gactacgccg cgcttgtgca tgcgtacgag
     2041 gaaaccgaga ccgacgaggt ggaaggggag ggtgacgatg actgacgctc gtccggccgg
     2101 cgctcgtccc gctgacgacc ttccggccgg cgcatggtcg gtgctcaagc gcggcctcgc
     2161 cgaatcgccc gaacttcgcc gcggggtcaa gagttcggca gccgtcggca ttctcatcgc
     2221 catcagtcgc ctggtcatcc cgatcgccgt ccaacagatc atcgaccgcg gcatcaccgg
     2281 ccccgacggc ttccggccgg gcttcgtcat cggctgctgt tcggcagcgc tcgccgcgct
     2341 cgtgctcgtg gccgtcggcg ggcgtttcac ctattaccgg atggcccgtg tcgccgagga
     2401 caccctttat ggcctgcgga cccgggcgtt cgatcacatc caccgtctca gcctcagcac
     2461 ccacaccgaa tcgaaacggg gagtgctcgt gagccgggtc acctccgaca tcgacaccct
     2521 cacgcagttc gccggttggg gagcgctgag ttggatcatg aactcggcgg tgctcgtcgg
     2581 tgtgctgctc gtgatgttcg tgtattcgt
//
LOCUS       DAOOMA010000397         2609 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_48543_length_2609_cov_2.834377, whole genome shotgun sequence.
ACCESSION   DAOOMA010000397 DAOOMA010000000
VERSION     DAOOMA010000397.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 2609)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2609)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2609
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48543_length_2609_cov_2.834377"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            36..>2609
                     /gene="dnaE"
                     /locus_tag="PLP95_10215"
     CDS             36..>2609
                     /gene="dnaE"
                     /locus_tag="PLP95_10215"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HPB46215.1"
                     /translation="MPALLGFANAFPDIQLDVSFSDRLANLVDEGFDAAVEKAARLQD
                     IFGRDNLFVEIQDHGIDDQIRTNPDLLRIAKKIQAPLLATNDSHYVHRDDAVSHDALL
                     CVQTGSLISDKDRFRFHGDEHYLKTAEEMRYLFREVEVACDNTLWVAERADVQIEFGD
                     ALLPNFPLPEGFADDSEYLRHLTFEGARLRWGDPLPDNVVERLAYELKVIADMGFSSY
                     FLITWDLIKHARDNGIRVGPGRGSAAGCAVAYTLWITDLDPIKYDLLFERFLNPSRIS
                     MPDIDMDFDTRYRDEMIRYAAEVYGRDHVAQIVTFSQIKARNAVRDAARVLGYPYAVG
                     DKVAKAMPPLIMGRDTPLDACLEKKPKYEEGYAMAAELRAMYEAEPDVKAVIDVAKGL
                     EGLRRSDGIHAAAVVITKDPLTTYLPIQRKPESGKPIEESPVVTQYEMGGVESLGLLK
                     MDFLGLRNLDVISDALELISTKRGIDLDIDNVSLEDAKTYELLGRGDTIGVFQLEGTA
                     MRALVRSLAPDKFEDVAALVALYRPGPMSVNMHNDYADIKNGRKAVSYFHPDAEDLLG
                     DTYGLMIYQESVMRVAQKFAGYSLAEADNLRKACGKKIRELMAKERAGFVEGVVNTGY
                     GEKLGTELFDIIERFADYAFNKSHSYGYGFIAYQTAYLKANFPAEYLSALLTSVKSSL
                     EKAAVYLAECRVMGITVTVPDVNRSDVDFVPYLNEESGDGAGASKESLEISFGLSGIR
                     GVGEALVEHIVRERRDNGAYVDFYDFCERVDMTVLNKRTVESLIKAGAFDSCGHPRQG
                     LLSVYEQVIDQTVARRKERDMGVMSLFGEVEDTETSFNELPRVPDVEFDLRSQDPRPK
                     KN"
BASE COUNT          503 a          796 c          841 g          469 t
ORIGIN      
        1 tgacagcccc ggtgtcgttc ggcacgctgc agctggtgcc ggctctgctc ggcttcgcca
       61 atgcgtttcc cgacatccag ctcgatgtca gcttttctga ccggctggcc aacctggtcg
      121 acgaggggtt cgacgcggcg gtggaaaagg cggcgcgcct gcaggacatt ttcggtcgcg
      181 acaacctgtt cgtggagatt caggaccacg gaatcgacga ccagatccgc accaatcccg
      241 acctgttgag gatcgccaag aagatccagg ccccgttgct ggcgaccaac gacagtcact
      301 atgtgcaccg tgatgacgcg gtgtcacacg acgcgctgtt gtgtgtccag accgggtcgc
      361 tgatcagcga taaagaccgg ttccggttcc acggcgacga gcactacctc aagaccgccg
      421 aggaaatgcg gtatctgttt cgcgaggtgg aggtggcgtg cgacaacacg ctgtgggtcg
      481 ccgagcgagc ggatgttcag atcgaattcg gcgacgcgtt gctgccgaac ttcccgttgc
      541 cggaaggctt tgccgacgac agcgaatatc tacgccacct gaccttcgag ggcgcacgcc
      601 tgcgctgggg cgacccgctg cccgacaacg tcgtggaacg cctggcctac gagctcaagg
      661 tcatcgcgga catgggcttc agctcgtact tcctcatcac gtgggacctc atcaaacacg
      721 cccgcgacaa cgggatccgc gtcggtccgg gacgtggttc tgcggcgggc tgtgcggtgg
      781 cttacacgct gtggatcacc gacctcgatc cgattaagta cgacctgctg ttcgaacggt
      841 tcctcaaccc gagtcgtatc tcgatgcccg acatcgacat ggacttcgac acccggtacc
      901 gagacgagat gatccgctac gcggccgagg tgtacgggcg tgatcacgtc gctcagatcg
      961 tgaccttctc ccagatcaag gcccgcaatg cggttcgcga tgcggcccgg gtgctcgggt
     1021 acccctatgc ggtgggcgac aaggtcgcca aggcgatgcc gccgctcatc atgggtcgcg
     1081 atacgcccct cgacgcgtgc ctcgagaaaa agccgaagta cgaagagggt tacgccatgg
     1141 cggccgagct tcgtgccatg tatgaggccg aacccgacgt gaaagcggtg atcgacgtcg
     1201 ccaaggggct cgaggggctg cgccgaagcg acggcatcca cgcggctgcg gtggtgatca
     1261 ccaaggatcc gctcacgacc tatctgccga ttcagcgcaa gcccgagtcg ggcaagccga
     1321 tcgaggagtc gccggtcgtc acccagtacg aaatgggtgg ggtcgagtcg ctcgggttgt
     1381 tgaagatgga cttcctgggg ttgcgcaacc tcgacgtcat cagcgacgcg ttggagttga
     1441 tctcgaccaa acgcggcatc gacctcgaca tcgacaacgt gtcgttggaa gatgccaaga
     1501 cctacgaact gttgggtcgg ggtgacacca tcggggtgtt ccaactcgag ggcaccgcca
     1561 tgcgcgcgct cgtgcgctcg ttggcgcccg acaagttcga agatgtggcc gcgctggtgg
     1621 cgctgtatcg ccccgggccc atgtcggtga acatgcacaa cgactacgcc gacatcaaga
     1681 acgggcgtaa ggcggtgtcg tatttccacc ccgacgcgga ggacctgctc ggcgacacct
     1741 acggcctgat gatctatcag gaatcggtga tgcgcgtcgc gcagaagttc gcgggctatt
     1801 ccctcgccga ggccgacaac ctgcgcaagg cgtgcggaaa gaaaatccgc gagctgatgg
     1861 ccaaggagcg ggcggggttc gtcgaggggg tcgtcaacac cgggtacggc gaaaagctcg
     1921 gcaccgaact gttcgacatc atcgaacgct tcgccgacta cgcgttcaac aagagccaca
     1981 gttacggcta cggcttcatc gcctatcaga ccgcctatct caaggcgaac ttccccgccg
     2041 agtacctgtc cgcgttgctg acctcggtga agagctcgct ggaaaaggcg gcggtgtatc
     2101 tcgccgaatg tcgggtgatg ggcatcaccg tcacggtgcc cgacgtgaac cgctccgatg
     2161 tcgatttcgt gccctacctg aacgaagagt cgggcgatgg cgccggcgcg tcgaaggagt
     2221 cgctcgagat ctcctttggg ctttcgggca ttcgtggcgt gggcgaggcg ctcgtggaac
     2281 acatcgtgcg tgagcgtcgc gacaacggcg cctacgtcga tttctacgac ttctgcgaac
     2341 gcgtcgacat gaccgtgctg aacaagcgca cggtcgagtc gctcatcaag gccggcgcct
     2401 tcgacagttg cggacatccc cgacaggggc tgctgtcggt ctacgagcag gtcatcgatc
     2461 agaccgtcgc gcggcgcaag gaacgcgaca tgggggtcat gagcctcttc ggcgaggtgg
     2521 aggacaccga gacctcgttc aacgagttgc cccgggtgcc cgacgtcgag ttcgacctga
     2581 ggagccagga tccgcgcccg aagaagaac
//
LOCUS       DAOOMA010000398         2603 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_48702_length_2603_cov_3.253532, whole genome shotgun sequence.
ACCESSION   DAOOMA010000398 DAOOMA010000000
VERSION     DAOOMA010000398.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 2603)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2603)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2603
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48702_length_2603_cov_3.253532"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..320
                     /gene="soxR"
                     /locus_tag="PLP95_10220"
     CDS             <1..320
                     /gene="soxR"
                     /locus_tag="PLP95_10220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="redox-sensitive transcriptional activator SoxR"
                     /protein_id="HPB46216.1"
                     /translation="QRRYHRDVLRRIAFIRAAQRVGISLAEIAAALTTLPRERTPTAR
                     DWARLSSHWRASLDQRIARLEQLRDTLDGCIGCGCLSLDACALFNPDDELSAFGTGAV
                     RFE"
     gene            complement(387..1232)
                     /locus_tag="PLP95_10225"
     CDS             complement(387..1232)
                     /locus_tag="PLP95_10225"
                     /inference="COORDINATES: protein motif:HMM:NF015338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HPB46217.1"
                     /translation="MIRCPWRTRAVAAVAIVAVVAVAGGATPASAGIPSPAVKPGANM
                     VVHMNLWGAKGNPDGGWNPDIAQAVLNSLKARTNPYPLVVSFNEICETQWLSLWNNYF
                     GLHSYVAEAHWGTTVSGTCGRFGNVVAVRGSAAIRSHGSFSNQATNQTGVGWVCVASN
                     LFNLTGCSAHVTARSAPPNIRQTQMWEYSVVANQYLTQNLPVWVAGDFNLEYAENQGA
                     FDSWWYAVLGEADAVSLARANKRKTTASRRIDYIFTRSPNVITDDAYIYDSPFSDHHW
                     YQMYN"
     gene            complement(1229..1963)
                     /locus_tag="PLP95_10230"
     CDS             complement(1229..1963)
                     /locus_tag="PLP95_10230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB46218.1"
                     /translation="MTRLTFDTKRWKITRTAELAHLGVQWGADLRGGVLDGVPMIRSV
                     GAGGFLLRPDSEVALPDACPVGSILYAAGMAPIGATSYGADVKPQSDEPQVVRRYGAD
                     GGDLDAITAPAVGPDEVATGLTVSCSSDALYLERSIANPTQGTSRFQWDVLDGEGWRT
                     VLMASVGSFVPNEYTDPSVVVKVRGEPGDNSAVELVALEGGRTTGKVASDDSRFSSYR
                     VIRLGDRRIGLVGAAEDSTFRFEEAP"
BASE COUNT          479 a          848 c          799 g          477 t
ORIGIN      
        1 accaacgccg gtaccaccga gacgtgctgc ggcggatcgc gttcattcgg gccgcgcaac
       61 gcgtcgggat ctcactcgcc gagatcgcag cggccctgac caccttgccg cgggaacgaa
      121 caccaaccgc tcgcgactgg gcacgccttt cgagccactg gcgggcgtcg ctcgatcagc
      181 ggatcgcgag actcgaacag ctacgcgaca ctctcgacgg ctgcatcggc tgcggatgcc
      241 tctcactcga tgcgtgcgct ctgttcaacc ccgacgacga gctttcggcc ttcggaactg
      301 gagccgttcg gttcgagtga tcgccgcggg cgccagacgg tggcttcact gcgccatcgt
      361 cgagcgagca cgcgcaccgc ccggcatcag ttgtacatct ggtaccagtg gtggtctgag
      421 aacgggctgt cgtagatgta cgcgtcgtca gtgatgacgt tgggtgatcg cgtgaaaatg
      481 tagtcgatgc ggcgacttgc ggttgtcttc cgtttgttcg ctcgggcgag ggatacggcg
      541 tctgcttcac ccaacaccgc gtaccaccag ctgtcgaatg cgccttggtt ctcggcgtac
      601 tccaagttga agtcaccggc aacccagacc ggaaggttct gggtcaggta ttggttcgcg
      661 acgacggagt actcccacat ctgcgtctga cggatattgg gcggggcgct tcgggcagtc
      721 acatgagcag agcagccggt gaggttgaag aggttggagg cgacacagac ccaaccgact
      781 cccgtctggt tggttgcctg attactgaac gagccgtggc tccggatcgc cgcggagcca
      841 cgaacagcga cgacgttccc gaatcgcccg catgtgcctg aaacggtagt gccccagtgg
      901 gcctctgcga cgtagctgtg caggccgaag tagttgttcc acaggctcag ccattgagtc
      961 tcgcaaatct cattgaacga cacgacaagg ggataggggt tggttcgcgc cttgagcgag
     1021 ttgaggaccg cttgagcaat gtcggggttc caaccgccgt ccgggttgcc tttggccccc
     1081 cacaggttca tgtgcaccac catgttggcg cctggtttca ccgcagggct cggaatcccc
     1141 gcgctcgccg gggttgcgcc gccagcgacc gcgacgaccg caacgatcgc gacagcggcg
     1201 accgctcgtg tacgccatgg acatcggatc atggcgcctc ctcgaagcgg aacgtggagt
     1261 cttcagccgc acccacgagc ccaattcgac gatcgcctag tcgaatgacg cggtacgacg
     1321 agaatcgcga atcatcgctc gcgactttgc cagtcgtcct cccaccctcc aacgcgacga
     1381 gctcgaccgc cgagttgtca ccgggctcac cgcgcacctt gacgaccacc gatggatcgg
     1441 tgtactcgtt tggaacgaac gaaccgacgc tcgccatgag cacggtacgc cagccctcac
     1501 cgtccagcac atcccactga aaccggctgg tcccttgagt tggatttgca atgctgcgct
     1561 ccagatagag agcgtcggac gagcaagaga cggtcaaacc ggtcgcaacc tcgtcgggcc
     1621 cgactgccgg ggcggtgatc gcgtccaagt cgccgccgtc ggcaccgtag cgtcgaacca
     1681 cttgcggctc gtccgactgc ggcttcacgt ctgccccgta gctggtcgct ccgatcggcg
     1741 ccatgcccgc ggcgtaaagg atcgacccaa ccggacatgc gtccggcagc gccacttccg
     1801 agtccgggcg caacaggaac ccgcccgcac cgaccgagcg aatcatcgga accccgtcga
     1861 gcactccccc gcgcaggtcg gcgccccatt gcacaccgag atgcgcgagc tcggcggtac
     1921 gtgtgatctt ccagcgcttg gtgtcgaacg tgagacgcgt caacgcaacc tctccttcgc
     1981 agtaccgatc cccgtccgtg tcggtcgtca aggaagtcag gcaatgattt acaagcacat
     2041 cgaggcgcga ttcgcccggt gtggccacag cgccgagtga ttggatctcg cctttgatcg
     2101 gctcgcggag tcgctccttc tgtccttcgg gcgccagttc ggcaacagcg accgctgttc
     2161 tcacatcgct gatcgctagg agtcgatcac catccacgac aatacgcgtc gggcctccgg
     2221 cctcgaatgt cgatccagga attgagccct cctcgtcgac ctgaccgcac gacgtcaagg
     2281 ctatcgccat cgcgaacacc gctactcgac gtcgatagcg acctctgacg tttgcagcca
     2341 tggcgaacgc ctccttcaat gatgcaaggt ctcggcactc tatcgacggg atcagtgccc
     2401 cgcctgccga atcgcagtgc ctgagcctca gctcaagggc atgcgtgtgg gaccactggc
     2461 agcgagacgg cggtgggtgg cgggacgatg ccgccggggt tgttgaagaa gaagacggtg
     2521 ccgatcgctg gaacgtcggg ttcgccgtcg tatggtgaca cgacctacgc gtatgaccag
     2581 tacggcagcc tggcggtcat ggt
//
LOCUS       DAOOMA010000399         2601 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_48775_length_2601_cov_3.280833, whole genome shotgun sequence.
ACCESSION   DAOOMA010000399 DAOOMA010000000
VERSION     DAOOMA010000399.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 2601)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2601)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2601
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48775_length_2601_cov_3.280833"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..691)
                     /locus_tag="PLP95_10235"
     CDS             complement(<1..691)
                     /locus_tag="PLP95_10235"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HPB46219.1"
                     /translation="MTDIWIGFDADDTLWHNEHYFVESYDLFAELVAPYLEADHADPR
                     QAAHDALIETERRNVPSFGYGIKGATISMIEAAIAISHGEIDTSQILRLVERAKAMMH
                     HPVEILDGVHDALDALSCHRLVLLTKGDLKDQHRKIDASQLAERFDAVEIMHEKDSDT
                     YAKVLQRHGIDSAGFVMVGNSLHSDVWPVLGLGAGAVHVPYHTTWALETAEDPLDHPR
                     YRRTSTLHDVPR"
     gene            925..1923
                     /locus_tag="PLP95_10240"
     CDS             925..1923
                     /locus_tag="PLP95_10240"
                     /inference="COORDINATES: protein motif:HMM:NF013916.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB46220.1"
                     /translation="MSGGGTLGYRRASGVGAGAFTTLSVRPVGAPVPVLSTGPGPAEL
                     GRVALEAVRFGSVDAAGLERRAEELGGRVVTGSSLAAGLDLTIRCMDLVGPENLESAS
                     SVAALASRAGEPDRSDPACPAIAAVRVDPRFVAAVTDALAGSSVRVVSGTARIAIGEH
                     RPSQRLVEIGETVTAGAHEVDIVFEGGQRYSDGIDRWYEAIVAATRACAPAKLNVGLE
                     LGGLDGYQEVRRASMVAMAAGADMIRIATGTRTPRASQAAVLCVSEAISDFAVQSGRR
                     VGLKVDGNALGVEQALRYLVIVNETLGIAWLRPDWFRLASTGLLDEVVEQRRTGRS"
     gene            1920..>2601
                     /locus_tag="PLP95_10245"
     CDS             1920..>2601
                     /locus_tag="PLP95_10245"
                     /inference="COORDINATES: protein motif:HMM:NF012398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HPB46221.1"
                     /translation="MTPGDPTFNATRSAGGFADYSPALENRRRVVIREVYAHVIDGAF
                     VPSASSKTLTVVEPATGESLAEVASGNAADVDLAVRAARRAFDRLWNDTDPLERGRHL
                     FRLSQVIRRRCREFSVLGAIESGRPVRWLRETDGPAAVAQIFHFAGWADKFDGAVHNP
                     PARSVAVAMVEPLGVMGILAPPGRHVAIHAVDGDIEPAAREPRRLRHAQAIRARGRGR
                     SRVRSVPRL"
BASE COUNT          407 a          782 c          930 g          482 t
ORIGIN      
        1 cgcggggaac gtcatggagc gtcgatgtgc ggcggtaccg cgggtggtcc aacggatcct
       61 cggcggtctc gagcgcccag gtggtgtgat aggggacgtg aactgccccg gccccaagcc
      121 caagcaccgg ccacacgtcc gaatgcagtg agtttccgac catcacgaag ccggcggagt
      181 cgatcccgtg acgttgaagg acctttgcgt aggtatcgct gtccttttca tgcatgattt
      241 cgacggcgtc gaaacgttcc gcaagctggg aggcgtcgat cttgcgatgt tgatccttta
      301 gatcaccctt ggtgagcaac accaggcggt ggcaggaaag ggcgtcgagc gcgtcgtgga
      361 cgccgtcgag gatttccacc ggatggtgca tcatcgcctt ggcgcgttcg accagccgaa
      421 gtatctgtga tgtgtcgatc tcgccatgcg agatcgcgat agcggcttcg atcatcgaga
      481 tcgtcgctcc cttgatgcca tagccgaagc tcggaacgtt gcggcgctcg gtctcgatca
      541 aggcatcatg ggccgcttgg cgtgggtcgg cgtgatcggc ttcgaggtaa ggcgcgacga
      601 gttcagcgaa gaggtcgtag gactcgacga agtagtgctc gttgtgccac aaggtgtcgt
      661 cggcgtcgaa accgatccag atgtcggtca ccgctcaagt gtcgctcacg cgccagttct
      721 gcgcaccctc gcgaggatgg cggtggctca gctcgagccg cccggcaggc gccgatgact
      781 ggctcccccc cgagaggatt agtctttatg cggtgtggcg tcgctggggc gccatgacga
      841 ggatgtggcg atggcgagcg cgtatgggcg ggcacgtggc accgcggagg tcggcggatc
      901 gtcgagggcc gtcggtggtt ttcgatgagc ggcggtggaa ccctgggcta tcggagagct
      961 tctggggtcg gggctggagc gttcacgacc ttgtcggtga ggccggttgg cgctccggtt
     1021 ccggtgttgt cgaccggacc cggaccagcc gagcttggcc gtgttgcgtt ggaggcggtt
     1081 cgattcggct cggtcgacgc tgctgggctt gagcgccgcg cagaagaact gggcggccgg
     1141 gtcgttacgg gatcctcgct cgctgcaggt ctcgatctga cgattcgctg tatggacctg
     1201 gtcggtccag agaatctcga gtcggcatcg agcgtcgcag cgcttgcatc acgcgccggc
     1261 gaaccagacc gctccgaccc cgcgtgtccg gcaatcgctg cggtgcgtgt cgacccccgc
     1321 ttcgtcgcgg ccgtgaccga cgctctcgca gggtcctcgg tgcgggtcgt gtcgggcacc
     1381 gcaaggattg cgattggcga gcatcggcca tcgcagcggt tggtcgaaat cggtgagacc
     1441 gttaccgctg gagcccacga ggtcgacatc gtcttcgaag gcgggcagcg gtattcggac
     1501 ggtatcgatc gatggtacga agcgatcgtg gccgcgacgc gtgcgtgcgc gccggcaaag
     1561 ctcaacgtcg gtctcgagct cggtgggctc gacggatacc aggaagttcg gcgcgcctcg
     1621 atggtggcga tggcggccgg ggccgacatg atcaggatcg ctaccgggac ccgtacacca
     1681 cgagcgtcgc aggccgcagt cctctgcgtg agcgaggcga tcagcgattt cgcagtgcag
     1741 agcggacggc gagtcggttt gaaggtcgac ggcaacgcgc tgggtgtcga gcaggcgctg
     1801 cgctatctgg tgattgtcaa cgagacgttg ggaatcgcct ggctccgccc cgattggttt
     1861 cgcctcgcat cgacgggcct gctcgatgag gtagtcgagc aacgtcggac ggggcgttca
     1921 tgaccccagg cgaccccacc ttcaacgcca cgaggtccgc cggcggtttc gccgactact
     1981 cgccggcgct tgagaaccgg cgacgtgtcg tcatacgtga ggtctacgcc cacgtgatcg
     2041 acggggcatt cgtgccatcg gccagttcga agacgctcac ggtcgttgag ccggcaacgg
     2101 gtgaatcact cgcggaggtg gcctccggga atgccgcgga cgtcgacctc gcggttcggg
     2161 cggcgcgtcg tgcattcgac aggttgtgga acgacacgga cccgctcgag cgcggcaggc
     2221 acttgtttcg cctctcgcag gtgatacgac ggcgatgccg cgagttctcg gtccttggag
     2281 cgatcgaaag cggccgaccg gtgcgttggc tgcgggagac cgacgggccg gcggcggtgg
     2341 cgcagatctt ccatttcgct ggttgggccg acaagttcga tggcgccgtc cacaacccgc
     2401 ctgcacgcag cgtcgccgtc gccatggtcg agccgctcgg ggtcatgggc attctcgccc
     2461 cgcccggccg ccacgtggcg atccacgcag ttgacggcga cattgagcct gccgcccgcg
     2521 aaccacgtcg cctccggcac gctcaggcca tccgggcccg gggtcggggg agatcacgtg
     2581 tgcgcagtgt gccaaggctg c
//
LOCUS       DAOOMA010000400         2592 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_49014_length_2592_cov_4.108396, whole genome shotgun sequence.
ACCESSION   DAOOMA010000400 DAOOMA010000000
VERSION     DAOOMA010000400.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 2592)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2592)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2592
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49014_length_2592_cov_4.108396"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..473
                     /locus_tag="PLP95_10250"
     CDS             <1..473
                     /locus_tag="PLP95_10250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB46222.1"
                     /translation="NLRFTHAGLQSASPQSIEMTDLAWDQYFATLRVYLRDFAHLVPA
                     YIEAEAPTQAFGEAPSAAWNNLRAALGLDDDANVGASVRIKRVGVDPLEGTVDYLTER
                     FVGVAGRDFLVRFHRREAIGLPIAVAHHQYLDPDHAVLDVESERLAWIDWLAEL"
     gene            complement(770..1285)
                     /locus_tag="PLP95_10255"
     CDS             complement(770..1285)
                     /locus_tag="PLP95_10255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB46223.1"
                     /translation="MKPASRHPLVLSYYSELRVKCSDLDDEPRRALLGEAVQQLNAAT
                     RGVRDDEMESVLAAFADPADVVAASGRLQAIEEAQGVGTGAVPLYLAIVGLAFVFIPW
                     IGAAIGALAVVAGLVTLVRRRAAGLSIGVPLGAIGVGVLAVVLPIVLLIVLNNIQDDQ
                     STPTPKVVELR"
     gene            complement(1398..2111)
                     /locus_tag="PLP95_10260"
     CDS             complement(1398..2111)
                     /locus_tag="PLP95_10260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012230583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HPB46224.1"
                     /translation="MADQASRPRSFGQLSPEERPGPGESIVVLGLGRFGAAVASELVR
                     LGYDVLGVDEVESLVQTYSRHLTHTVEADVTNVDAIRQLGIDEMTHAIVAVGSDIESS
                     ILATAVLDEVGVPNIWAKAVSKQHARILERVGAHHVVLPEHDMGHQVAHMIGGRMLDW
                     FQLDERFAMVETVVPAELVGKTLTEAAIRQRWNVTVVCVKPSRGNFTYANAETVLGKG
                     DLLVVAGETESAEAFARVH"
     gene            complement(2104..>2592)
                     /locus_tag="PLP95_10265"
     CDS             complement(2104..>2592)
                     /locus_tag="PLP95_10265"
                     /inference="COORDINATES: protein motif:HMM:NF014443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter TrkG"
                     /protein_id="HPB46225.1"
                     /translation="AQIANPNSAKVDTVMLMFVGGGSSSTAGGIKVSTFALLGLAIWA
                     EVRGDRDTTIFHRRVPSNLQRQAVAVALLAVGAVMAATLAMLALTPFGLSEVLFESVS
                     ALATVGSSTGITPTLGAGAKILMIVLMFLGRVGPPTLFAALVLRERERLYRYPEERIV
                     IG"
BASE COUNT          484 a          943 c          744 g          421 t
ORIGIN      
        1 ccaatctgcg gtttacccac gcgggccttc agagcgcctc gcctcagtcc attgagatga
       61 ccgacctcgc gtgggaccag tacttcgcga cgcttcgggt ttacctgcgc gatttcgcgc
      121 acttggttcc tgcctacatc gaggccgagg ccccaacaca ggccttcggc gaagcaccct
      181 cggcggcttg gaacaatctc cgcgcagcgc tcggactcga cgatgacgcc aacgttggag
      241 cctcggtgcg gatcaagcgg gtcggggtcg acccgctcga gggcaccgtc gactacctca
      301 ccgaacgttt cgtcggggtg gcaggccgcg acttcctcgt acggttccat cgacgcgagg
      361 ccatcgggct accgatcgca gtggcccatc accagtacct cgaccccgat cacgccgtcc
      421 tcgacgtcga gtcggagcgc ctcgcatgga tcgactggct cgccgagctc tgaccgttgc
      481 cctccctgcc ggagcctgcc aaccggtgtc cggtccgtgt cggcggccgg caccacctcg
      541 gcaccacgcc gcgatacggt catgacatgc gcaacaagat cgtcatcgca ctcatcccga
      601 tcctcgccgc gttcatcgca aagcagctgc aggactacct gcgtaacaaa gaggcagcaa
      661 aaaccgccgc ctgaacgcga cccctggcgg gtcgctccag ccggccatcg ccccacagcg
      721 ccggtcggct caacccgcgt ccgtaccgcg ccggtcagct cagcccgggt cagcgcagct
      781 cgacgacctt cggggtcggc gtcgactggt cgtcttggat gttgttcagc acgatcaaca
      841 acacgatcgg cagcacaacg gccaacacgc ccaccccgat cgctccaagg gggacgccga
      901 tcgacagccc tgccgcgcgg cggcgaacca gcgtcacgag gccggctacc accgctaaag
      961 caccgatcgc cgcgccgatc cacgggatga acacgaacgc cagcccgacg atcgccaggt
     1021 acaacggcac tgcgcccgta ccgacccctt gcgcctcctc gattgcctgc aatcgtcctg
     1081 aagcggcgac cacgtcggct gggtcggcaa acgcggcgag gaccgactcc atctcgtcgt
     1141 cgcggacacc gcgcgtcgcc gcgttcaact gctgaaccgc ctcgcccaac agcgcacgac
     1201 gtggctcgtc gtcgaggtcc gagcacttca cgcgcaactc ggagtagtaa ctcaacacaa
     1261 gcggatgtcg cgacgctggt ttcatcgaga ccattcttcc agcgtttcac caaaacgcgg
     1321 tcgaaccccc ggcattgccg ggttcacatc cgatcggacg ctgccccgct cgcctgattc
     1381 accgctccgg cgcttgatca gtggacccgg gcgaacgcct cggcggattc ggtctccccc
     1441 gccaccacca acaggtcgcc cttgccgagc acggtctcgg cgttggcgta ggtgaagttg
     1501 cctcggctcg gcttcacaca cacaacggtg acgttccaac gctgacgaat cgccgcttcg
     1561 gtgagcgtct ttccgaccaa ttccgccgga acgaccgttt ccaccatggc gaagcgctcg
     1621 tcgagctgga accaatccag catccgtcca ccgatcatgt gggcgacctg atggcccatg
     1681 tcgtgttcgg gcaacaccac gtgatgcgcg ccgacacgct cgaggatccg agcgtgctgt
     1741 ttgctgaccg ccttcgccca gatgttgggg acgccaacct catcgagaac cgccgtggcc
     1801 aggatgctcg actcgatatc ggacccgacc gccacgatcg cgtgagtcat ctcgtcgatc
     1861 ccaagctgac ggattgcgtc cacgttggtt acgtcagcct cgacggtatg ggtcaggtgc
     1921 ctgctgtagg tctgcaccaa cgactcgacc tcgtcgacgc cgagaacgtc gtaaccgagc
     1981 cgcaccaact ccgacgcaac ggcggcgccg aatcggccca ggcccaacac gacgatcgat
     2041 tcgcccggac cggggcgctc ctccggggac agttgaccga agctgcgcgg cctcgacgct
     2101 tgatcagcca atgacgatcc tctcctcggg atatcgatac aaccgttctc gttcgcgcaa
     2161 caccagtgcc gcgaacaacg tgggtgggcc gactcggccc aggaacatca acacgatcat
     2221 caagatcttc gcgcccgcgc cgagtgtcgg agtgattccg gtcgatgacc cgaccgtggc
     2281 cagagccgac accgactcga acagcacctc cgagaggcca aatggtgtca acgccaacat
     2341 cgccagcgtc gcggccatca ccgccccgac cgccaggagc gccaccgcga cggcctgacg
     2401 ttgcaggttc gacggaaccc tccgatggaa gatcgtggtg tcgcgatcac ctcgcacttc
     2461 ggcccagatc gccaacccca acagcgcgaa ggtcgacacc ttgatgccgc cggcggtcga
     2521 actgctgccg ccacccacga acatcagcat cacggtgtcg accttcgcgc tgttggggtt
     2581 ggcaatctgg gc
//
LOCUS       DAOOMA010000401         2590 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_49054_length_2590_cov_4.224852, whole genome shotgun sequence.
ACCESSION   DAOOMA010000401 DAOOMA010000000
VERSION     DAOOMA010000401.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 2590)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2590)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2590
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49054_length_2590_cov_4.224852"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(21..317)
                     /locus_tag="PLP95_10270"
     CDS             complement(21..317)
                     /locus_tag="PLP95_10270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015798268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WhiB family transcriptional regulator"
                     /protein_id="HPB46226.1"
                     /translation="MAQAKNTDEGNGWQLAANCLGVDPDLFFPERGASTKEAKAVCQG
                     CEVRDDCLEYALVNGEKFGIWGGLSERERRRIRRQRALARAAEASAETTRTATA"
     gene            complement(437..2452)
                     /locus_tag="PLP95_10275"
     CDS             complement(437..2452)
                     /locus_tag="PLP95_10275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HPB46227.1"
                     /translation="MSHRRFRYSPWDGTQSGFELDALDVMGQLTDDLLYHGDVNAGLR
                     RLLQEGFEDRNGDHVQGLREMMERLREARRERLENNSLGGVFDDIARELRELVDDERS
                     SLDRLAQEAAASDDERREALTNNDVAERNMRLEMLPNDLAGQVKDLQNYDFTSESAQN
                     RFDELVDRLREQLMQQTLDQMSSAVQDMTPEDMARLKDMMAELNNMLAQRAAGEEPDF
                     DSFMERYGDFFPENPSTLDELLEVMAQRMAAMQAMLNSMTPEQRQQLMDLSNQLLEDM
                     DLRWQMEQLSSNLQQLFPDAGWGRSYDFDGVDPLDFPSAMQMMSDLGDLDQLENLLGQ
                     AMSPASLGEVDLDRARELLGHDTAESMRKMSELSKMLADSGMVDTVDGRLELTPEGLR
                     RLGRNALDELFSKLGEHLAGEHQIRRTGIGHERTYDTKAYEWGDPFNLRINSTIENAV
                     RRRASQGLVGGRVQLTPDDFEIEQTEQSVRTATVLMLDLSMSMPMRGNFLPAKKVAMA
                     LHALISMQFPRDFLGLVSFSRLAHVFPAKDLPSVTWDYDYGTNMQHGLALARQLLARE
                     HGTKQIIMITDGEPTAHIDSYGRSIFDYPPTRDCIDATLLEVSRCTREHITINTFMID
                     ADSGLQHFVDRMTELNRGRAFFTTPENLGDFVLHDFLDQRRRLLNRR"
BASE COUNT          421 a          818 c          841 g          510 t
ORIGIN      
        1 cgggcggtca ggcggtgctg ctaggcggtt gccgtgcggg tggtttcggc cgatgcctcc
       61 gccgcccggg ccaacgcacg ctgacgccgg atccggcgcc gttcccgctc gctcagccca
      121 ccccagatac cgaacttctc accgttgacg agtgcgtatt cgaggcagtc gtcgcgcact
      181 tcacaacctt ggcacacggc cttggcttcc ttggtcgatg cgccgcgttc ggggaagaag
      241 aggtcggggt caacaccaag gcagttggcc gccagttgcc aaccgttgcc ttcatccgtg
      301 ttcttcgctt gtgccattcc cgtcctcacc gtgacgtaat tacatccctg ttatccggaa
      361 catcttgacc ggagtcgtca agaatcgcaa ggccgacatc gagaagttca cacacgcccc
      421 tcatccgccg ccgccctcag cgacggttca acaggcggcg gcgctgatcg aggaagtcgt
      481 gaagcacgaa gtcgcccaga ttctcggggg ttgtgaagaa cgcgcgacct cggttgagtt
      541 cggtcatccg gtcgacgaaa tgctgcaatc cggagtccgc atcgatcatg aaggtgttga
      601 tcgtgatgtg ttcacgggtg catctcgaca cctcgagcag ggtcgcatcg atgcaatcgc
      661 gggtcggtgg ataatcgaag atcgaacgcc cgtacgaatc gatatgggcg gtcggttcgc
      721 cgtcggtgat catgatgatc tgtttggtgc cgtgttctcg tgccaacaac tgtctggcca
      781 gggccaaacc gtgttgcatg ttggtgccgt agtcgtaatc ccaggtgacc gacgggaggt
      841 cctttgccgg gaagacgtgg gcgagccgac tgaagctgac gaggccgaga aagtcgcggg
      901 gaaactgcat ggagatgagc gcatgcagcg ccatcgccac cttcttggcc ggcaggaagt
      961 tgccgcgcat cggcatcgac atcgacaggt ccaacatcaa cacggtggcg gtgcggacgc
     1021 tctgttctgt ctgttcgatc tcgaagtcgt cgggggtgag ttgcacccgg ccgcccacca
     1081 gcccttggga tgcacggcgg cgcaccgcat tttcgatcgt ggagttgatg cgcaggttga
     1141 acgggtcgcc ccattcgtag gccttggtgt cgtaggtccg ctcgtggcca atgccggttc
     1201 gccggatctg gtgctccccc gcaaggtgct cgccgagttt ggaaaacagc tcgtcgagcg
     1261 cattgcggcc gaggcgtcgc aggccctcgg gggtgagctc gaggcgtccg tcgacggtgt
     1321 ccaccatgcc cgagtcggcg agcatcttgg acagttcgct catcttgcgc atcgactcgg
     1381 cggtgtcgtg tccgagcagc tcgcgggccc ggtcgagatc cacctcgccg agggaggccg
     1441 gcgacatcgc ctgaccgagc aggttctcga gctgatcgag atcgccgagg tcgctcatca
     1501 tctgcatcgc ggaggggaaa tcgagcgggt cgacaccgtc gaagtcgtag ctgcgccccc
     1561 acccggcgtc ggggaacagc tgctgcaggt tcgacgagag ctgctccatc tgccagcgca
     1621 ggtccatgtc ttcgagcagc tgattcgaca ggtccatgag ctgctggcgc tgctcggggg
     1681 tcatcgagtt gagcatcgcc tgcatcgccg ccatgcgctg ggccatcacc tccagcaact
     1741 cgtcgagggt gctcggattc tccgggaaga agtcgccgta gcgctccatg aaggagtcga
     1801 agtcgggctc ctcgccggcc gcacgctggg cgagcatgtt gttgagctcg gccatcatgt
     1861 ccttgaggcg cgccatgtcc tcgggggtca tgtcctgcac ggcggaggac atctgatcga
     1921 gcgtctgttg catgagctgc tcgcgcaggc gatcgacgag ttcgtcgaag cggttctgcg
     1981 cggactcgga ggtgaagtcg tagttctgta ggtccttcac ctgcccggcg aggtcgttcg
     2041 gcagcatctc gaggcgcatg ttgcgttcgg cgacgtcgtt gttcgtgagc gcctcgcgcc
     2101 gttcatcgtc ggatgcggcc gcctcctgag cgagacgatc gaggctggaa cgctcgtcgt
     2161 cgacgagttc gcgcaactcg cgggcgatgt cgtcgaacac gccgcccagg ctgttgttct
     2221 cgagccgttc gcgccgggcc tcccggaggc gctccatcat ctcacgcagg ccctgaacgt
     2281 ggtcgccgtt gcggtcttcg aatccctcct gcagcaggcg acgcagcccc gcgttcacgt
     2341 ccccgtgata gagcaggtcg tcggtgagtt gccccatgac gtcgagcgcg tcgagttcga
     2401 aaccgctttg ggttccgtcc cagggcgagt agcgaaagcg ccggtgcgac atgtcgagac
     2461 gctaccgcgc cagccccgcc cgttcccccg cgcccccgcc agttcccgcg cgcccccgcc
     2521 agttcccgcg cgcccccgcc agttctccga taaccagtgg tcgtgatcac gaccactggt
     2581 tatcggagaa
//
LOCUS       DAOOMA010000402         2587 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_49143_length_2587_cov_3.002765, whole genome shotgun sequence.
ACCESSION   DAOOMA010000402 DAOOMA010000000
VERSION     DAOOMA010000402.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 2587)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2587)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2587
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49143_length_2587_cov_3.002765"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..252)
                     /locus_tag="PLP95_10280"
     CDS             complement(<1..252)
                     /locus_tag="PLP95_10280"
                     /inference="COORDINATES: protein motif:HMM:NF013854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009143 - nucleoside triphosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-canonical purine NTP pyrophosphatase"
                     /protein_id="HPB46228.1"
                     /translation="MSATPVASATANPKKLVELQVLFDEVAAGCFELVPRPADLPDVE
                     ETGDTLGDNARLKAVAVMEATGCAAIADDIAYNNHDVEDG"
     gene            complement(263..1003)
                     /gene="rph"
                     /locus_tag="PLP95_10285"
     CDS             complement(263..1003)
                     /gene="rph"
                     /locus_tag="PLP95_10285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015799146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004549 - tRNA-specific ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HPB46229.1"
                     /translation="MRPDGRRPDELRPITIERDFTTTSHGSVLVAFGETRVLCTATVE
                     LDVPRWMKGTGKGWVTAEYSMLPGSSPERIRRERKGPKGRTQEIERLIGRSLRAVVDM
                     KALGERQVLLDCDVLQADGGTRTASISGAWIALHDACERMVQAGIIKANPVVDNCAAI
                     LVGIIDGEPRLDLPYVEDSTAQTDMNVVMTGAGGLVEVQGTAEDSPFSRAELNALLDL
                     AEGGIAEIVARQREVVAAAPTPKSFTAR"
     gene            complement(1015..1860)
                     /gene="murI"
                     /locus_tag="PLP95_10290"
     CDS             complement(1015..1860)
                     /gene="murI"
                     /locus_tag="PLP95_10290"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HPB46230.1"
                     /translation="MVVPANPRLDADAPIAMFDSGFGGLTVARAVIDLLPHEPIVYLG
                     DTARYPYGPRSQDEVRAFSHQIAEHLVDECGAKLLIVACNTAAAAALDELQDRFDIPV
                     IGVIEPGVRSVLKATENRRVGVIGTVGTIESGAYQDTVADHAACTGLDDVELCALSCP
                     GFVEFVERGEFDSHQVHVLAERLLAPAVEFGVDSLLLGCTHYPYLARTIAEVMGREVV
                     LVSSADETAFEIADLLVERGLDRDEAVPTPAPRFLTTGDVDWFQHLGSRLLGPELERA
                     ERVTL"
     gene            1951..>2587
                     /locus_tag="PLP95_10295"
     CDS             1951..>2587
                     /locus_tag="PLP95_10295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HPB46231.1"
                     /translation="MKVQLNVLDFLTRAETVYGDRVAVIDEPDQPAPPLGPLTYRQVG
                     EHARAMAAGLDALGIDRGDRVAIVSHNSARLLVAFFGVCGWGRVAVPVNFRLAAEEVR
                     YIVEQSGARVLLIDPELADSLADVDCEHRFIIGDESDAALFRFGVDPVPWEADEDAIA
                     TINYTSGTTARPKGVECTHRQEWIHASVAGWTLGVNDRDVYLHTLPMFDCNG"
BASE COUNT          426 a          885 c          844 g          432 t
ORIGIN      
        1 cccgtcttcc acatcgtgat tgttataggc gatgtcatcc gcgatcgcgg cgcatccggt
       61 cgcttccatc accgcaaccg ccttgagccg ggcgttgtcg cccaacgtgt cgccggtctc
      121 ctccacgtcg ggcaggtcgg ccggccgagg caccaactcg aagcatccag cggccacctc
      181 gtcgaagagc acctgcaact cgacgagctt cttggggttc gcggttgccg atgccaccgg
      241 ggtcgcgctc atcactgcgc ccctaccggg cggtgaacga cttcggcgtc ggagcggccg
      301 cgaccacctc gcgttgacgg gcgacgatct cagcaatgcc accctcggcc aggtcgagca
      361 gcgcattgag ttcggcacgt gagaacggcg agtcctccgc ggtcccctgc acctcgacga
      421 gcccgccggc accggtcatc acgacgttca tgtcggtctg tgcggtcgag tcctcgacgt
      481 agggaaggtc gagtcggggt tcgccgtcga tgatccccac caagatcgcc gcgcagttgt
      541 cgaccaccgg attcgccttg atgatgccgg cctgcaccat gcgttcacac gcgtcgtgta
      601 gggcgatcca ggcacccgaa atcgaggcgg tgcgggtgcc gccgtcggcc tgcaacacgt
      661 cgcagtccaa caacacctga cgttcgccga gtgccttcat gtcgaccacc gctcgcagcg
      721 atcgtccgat cagccgctcg atctcctggg tgcggccctt gggccctttg cgttcgcgac
      781 ggattcgttc gggggacgag ccgggcagca tcgagtactc ggcggtgacc cagccctttc
      841 cggtgccctt catccaacgc ggcacgtcga gttcaaccgt cgccgtgcac aacactcggg
      901 tctcgccgaa ggcgacgagc acagagccgt gcgaggtggt ggtgaagtcg cgttcgatgg
      961 tgatggggcg caattcgtcg ggtcgacggc cgtctggtct catgggcccc gacgttacaa
     1021 ggtgacccgc tccgcccgtt ccaactcggg tccgagcagg cggctgccga ggtgctggaa
     1081 ccagtcgacg tcgccggtcg tcaaaaaccg gggtgccggg gtgggcaccg cctcgtcgcg
     1141 atcgagcccg cgctcgacca gcagatcggc gatctcgaag gccgtctcat ctgcgctcga
     1201 caccaacacg acctcgcgcc ccatgacctc ggcgatcgtg cgggccaggt acgggtagtg
     1261 ggtgcagccg agcaggaggc tgtcgacccc gaactcgacg gcgggcgcga ggagtcgctc
     1321 ggcgagcacg tgtacctgat ggctgtcgaa ctcgccccgc tcgacgaact cgacgaaacc
     1381 gggacacgac agcgcgcaca actccacgtc atcgaggccg gtgcaggccg cgtgatccgc
     1441 aaccgtgtcc tggtaggcgc cggattcaat cgtgcccacg gtgccgatca ccccgacgcg
     1501 gcggttctcg gtcgccttga gcaccgaacg aacccccggc tcgatgacgc cgatgaccgg
     1561 gatgtcgaag cggtcctgca actcgtcgag cgctgccgcc gccgcggtat tgcaggcgac
     1621 gatcaacagt ttcgccccgc attcgtcgac gagatgctcg gcgatctgat gggagaacgc
     1681 ccgcacctcg tcttgggaac gcggaccgta ggggtagcgc gcggtgtcgc ccaggtacac
     1741 gatcggctcg tggggcaaca ggtcgatgac cgcccgggcc acggtgagcc caccgaaccc
     1801 cgagtcgaac atggcgatcg gcgcgtcggc atcgaggcgg ggattcgcgg gcacgaccat
     1861 agccaccaag ctaccgaccg cgactgcgtg gcaagatacg gccatcgacg ccggtctcgc
     1921 agggggacgg ccaattccgt ggagcgctca atgaaggtgc aactcaacgt cttggacttc
     1981 ctcacccggg ccgagaccgt gtacggcgac cgggtcgcgg tcatcgacga acccgaccag
     2041 ccggcaccgc cgctcgggcc gctgacgtat cgccaggtcg gcgaacacgc acgagcgatg
     2101 gccgccggac tcgacgcgct cggcatcgac cgcggcgacc gcgtcgccat cgtcagccac
     2161 aattcggccc ggctcctcgt cgcgttcttc ggggtgtgtg gatggggccg tgttgccgtt
     2221 ccggtcaact ttcgcctcgc cgccgaggag gttcgttaca tcgtcgagca atcgggcgct
     2281 cgggtgttgt tgatcgatcc ggagttggcc gattcgctcg ccgatgtcga ctgcgaacac
     2341 cggttcatca tcggcgacga gtctgatgcc gcgctgttcc ggttcggcgt cgaccccgtg
     2401 ccatgggagg ccgacgagga tgcgatcgcc accatcaact acacctccgg gacgacggct
     2461 cgacccaagg gcgtcgagtg cacccaccgc caggagtgga ttcacgcctc ggtggccggt
     2521 tggaccctcg gggtgaacga ccgcgacgtc tatctccaca ccctgccgat gtttgactgc
     2581 aacgggt
//
LOCUS       DAOOMA010000403         2583 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_49255_length_2583_cov_3.149525, whole genome shotgun sequence.
ACCESSION   DAOOMA010000403 DAOOMA010000000
VERSION     DAOOMA010000403.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 2583)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2583)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2583
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49255_length_2583_cov_3.149525"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1889)
                     /gene="carB"
                     /locus_tag="PLP95_10300"
     CDS             complement(<1..1889)
                     /gene="carB"
                     /locus_tag="PLP95_10300"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HPB46232.1"
                     /translation="MPRRDDLQSILIIGSGPIVIGQACEFDYSGTQACRVLREEGYRV
                     ILANSNPATIMTDPDFADATYIEPLDADVLTRIIDKEQPDAVLPTLGGQTALNLAMEL
                     HARGVIGVDGTPEMIGANAQTIETAENREAFKEAMIEIGLKVPPSDTARSMPEAREVV
                     ERIGLPVVIRPAYILGGKGTGIATTPEEFDRIASYGLDASPITEILIERSIAGWKEYE
                     LEVMRDNNDNVVIVCSIENVDPMGVHTGDSITVAPAMTLTDREYQEMRDAAIACLRRV
                     GVETGGSNVQFAVDPANGDMVVIEMNPRVSRSSALASKATGFPIAKIAARLAVGYTLD
                     EITNDITTKTPACFEPAIDYVVTKVPRWAFEKFPGTSGVLGTQMQSVGEAMAIGRTFP
                     ESLQKALRSLENGRLGLNCDPGERVWDDLSDAQLLALTDRATPDRLFVVGAALERGLS
                     IESIHEACKIDPWFLDQMSLIVEERHALEAAAAQGRRPADLSAREWRRAKQLGFSDAQ
                     LAYLFSTAAAAATADVEAAVTEAAVTEEDVRAARKAAGVLPTFKTVDTCAAEFAAETP
                     YHYSAWEDENEVRPSSRPKVIILGSGPNRIGQGIEFDYCCVHASLALAGKYETIMVNC
                     NPETVS"
     gene            complement(2016..>2583)
                     /locus_tag="PLP95_10305"
     CDS             complement(2016..>2583)
                     /locus_tag="PLP95_10305"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016717 - oxidoreductase activity, acting
                     on paired donors, with oxidation of a pair of donors
                     resulting in the reduction of molecular oxygen to two
                     molecules of water [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="acyl-CoA desaturase"
                     /protein_id="HPB46233.1"
                     /translation="STVLVWHCTFLVNSMAHLVGTRRYATADSSRNNAFIAFVTLGEG
                     WHNNHHHLQSSCRQGFKWWEFDVTFYVLKVLSWLRIVRDIKKPNAKAMNSALISEGAF
                     DIGMYEYHMDKAMTRVAAATAACTGRADLEEARLEFTIRADDALAAARELGRQTRRDA
                     KRPEPAERAARGERGRGAQRSRDTLPVR"
BASE COUNT          398 a          809 c          908 g          468 t
ORIGIN      
        1 gagacggtct cgggattgca gttgaccatg atcgtctcgt acttgcccgc aagcgcgagg
       61 ctggcgtgca cacagcagta gtcgaactcg atgccctgac cgatccggtt gggcccggag
      121 cccaagatga tcaccttcgg acgtgacgac ggccggacct cgttttcgtc ctcccacgcc
      181 gagtagtgat atggggtctc ggcggcgaac tcggcggcgc aggtgtcgac ggtcttgaac
      241 gtcggcaaca ccccggcggc cttgcgggcc gcgcgcacgt cctcctcggt gaccgcggcc
      301 tcggtaaccg cggcctcgac atcggcggtc gcggcggcag cggcggtgga gaacagatac
      361 gcgagctgtg cgtcgctgaa cccgagttgt ttggcgcggc gccattcgcg ggcgctcaag
      421 tcggcggggc gccgtccctg ggccgccgcg gcctcgagcg cgtggcgttc ctcgacgatg
      481 agcgacatct gatcgagaaa ccaggggtcg atcttgcacg cctcgtggat cgattcgatg
      541 ctcagcccac gttcgagggc ggcgccgacg acgaagagac gatccggggt ggcgcggtcg
      601 gtgagcgcga gaagctgggc gtcgctcagg tcgtcccaga cccgctcgcc ggggtcgcag
      661 ttcagcccga ggcggccgtt ttcgaggctg cgcagcgcct tttgcagcga ttcggggaag
      721 gtgcgcccga tcgccatcgc ctcgccgacc gactgcatct gagtgccgag cactcccgag
      781 gtgccgggga acttctcgaa ggcccagcgt ggcaccttgg tcacgacgta gtcgatcgcc
      841 ggctcgaaac acgccggggt cttggtggtg atgtcgttgg tgatctcatc cagggtgtag
      901 ccgacggcga ggcgcgctgc gatcttggcg atcgggaacc cggtggcttt cgaggccaac
      961 gcggagctgc ggctcacccg agggttcatc tcgatgacga ccatgtcgcc gttggcgggg
     1021 tcgaccgcga actgcacgtt cgaaccgccg gtctccaccc cgacgcggcg caggcaggcg
     1081 atggcggcgt cgcgcatctc ctggtactcg cgatcggtga gggtcatcgc cggggcgacg
     1141 gtgatcgagt cgccggtgtg cacgcccatc gggtcgacgt tttcgatgga acacacgatc
     1201 acgacgttgt cgttgttgtc gcgcatcacc tcgagttcgt attccttcca cccggcgatg
     1261 gaacgctcga tcaggatttc ggtgatcggg ctggcgtcga ggccgtagga ggcgatgcgg
     1321 tcgaactcct ccggggtcgt ggcgattccg gtgcccttgc cgccgaggat gtacgcgggg
     1381 cgaatcacta ccggaagacc gatgcgttcg acgacctcgc gagcctccgg catcgagcgg
     1441 gcggtgtccg aaggcgggac cttcagaccg atctcgatca tcgcctcctt gaaggcctcg
     1501 cggttctctg cggtctcgat ggtctgcgcg ttcgcgccga tcatctcggg ggtgccgtcg
     1561 acgccgatga ccccgcgggc gtgcagttcc atcgccaggt tcagcgctgt ctgtccgccg
     1621 agggtaggca agaccgcgtc cggttgctct ttgtcgatga tgcgggtgag cacgtcggcg
     1681 tcgaggggtt cgatgtaggt ggcatcagcg aaatcggggt cggtcatgat cgtcgcggga
     1741 ttcgagttcg cgaggatcac gcggtatccc tcttcgcgaa gcacgcgaca cgcctgggtc
     1801 cccgagtagt cgaactcaca cgcctggccg atcacgatcg gcccggaacc gatgatcaag
     1861 atcgactgaa ggtcgtcacg acggggcatc tcatctcctg gcaggcaaaa agaaggcccc
     1921 cggaggggcc tgggactact tcgaatggga cgaaccccgc aacgggggct gaagggcatg
     1981 gcagagcggg gggttgaagc tgtaggccac gttcgtcacc gaacgggcag cgtatcacgg
     2041 ctgcgttggg cccctcggcc gcgctcgccg cgcgccgccc gttcggccgg ttctgggcgt
     2101 ttggcgtcgc ggcgggtctg gcgtccgagc tcgcgggcgg ccgcgagcgc gtcgtcggcg
     2161 cgtatcgtga actcgaggcg ggcttcctcg aggtcggcgc gcccggtgca ggccgccgtc
     2221 gccgccgcga cccgggtcat cgccttgtcc atgtggtact cgtacatgcc gatgtcgaag
     2281 gcaccctcgc tgatgagcgc cgagttcatg gccttcgcat tgggcttctt gatgtcgcgg
     2341 acgatacgca gccagctgag caccttcagc acgtagaagg tcacgtcgaa ctcccaccac
     2401 ttgaagccct ggcgacacga gctctgcagg tgatggtggt tgttgtgcca gccttcgccg
     2461 agggtgacga acgcgatgaa cgcgttgttg cggctcgaat cggcggttgc gtagcgacgc
     2521 gtgccgacca ggtgggccat cgagttcacc aggaacgtgc agtgccacac cagcaccgtc
     2581 gac
//
LOCUS       DAOOMA010000404         2575 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_49478_length_2575_cov_3.425000, whole genome shotgun sequence.
ACCESSION   DAOOMA010000404 DAOOMA010000000
VERSION     DAOOMA010000404.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 2575)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2575)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2575
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49478_length_2575_cov_3.425000"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(134..1327)
                     /gene="secF"
                     /locus_tag="PLP95_10310"
     CDS             complement(134..1327)
                     /gene="secF"
                     /locus_tag="PLP95_10310"
                     /inference="COORDINATES: protein motif:HMM:TIGR00966.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HPB46234.1"
                     /translation="MSSTRKKLYRVQTDFDFTGLWRWMMLVSAAATVVSVVVISIWGL
                     NLSIDFKGGAVWEVPSKHISSDQAEQVLESFDKLGGAKIQRITDAEGTSILRVQATAA
                     KDISESNEIRQALADAANSSAPDDAQIDVTDIGTNTIGPSWGKSITRDAVRALIVFFI
                     VIAGYMAWTLEWRMAVSGLLAVAHDVLITVGIYAIARFEVTPATVISILTILGFSLYD
                     TIVVYDRVRENAAKYDPSGKYTYNAIKRRSLNQVIMRSVNTSMVAILPPLAIFIVGGL
                     MFGQPTMMDFSLALIIGLAIGVYSSIGVAAPMLVWLKEREPAYSRVRSRARARGIEAE
                     ADHIPKVDSWEVGVVPEGATRRRETAKVGAVERSVDQAATTAELAQKYDRAVPPRPRK
                     QGKKR"
     gene            complement(1324..>2575)
                     /locus_tag="PLP95_10315"
     CDS             complement(1324..>2575)
                     /locus_tag="PLP95_10315"
                     /inference="COORDINATES: protein motif:HMM:TIGR00916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SecD/SecF family protein translocase subunit"
                     /protein_id="HPB46235.1"
                     /translation="DPAADPAADPAADPATEAPTTDPASPPTTVPNQFGIDVSDEDFL
                     ELLGLEYSIEAASSGITAPEDDKADQPVTLFGTPDKDGNRVIYQLGPTLLTGRAIEDA
                     TPSLQNGQWVVMPKFKTGADGIDLFNQAAAQCYAGQPTCPAVGAEAGQLGVVLDGEVI
                     SAPVIQAASYTRDQITISGNSAAGGFTEQEARDLSLALRYGSLPIELEPQTVQFVSAT
                     LGEGALRAGIIAGLIGLAVVIIYMVAYYRILGLITIGSLLVTAAMLWAVVGFLGETQG
                     LALTLAGIVGIIVSIGVSLDSSIVYFENVKEDVSEGRNFRRAVEKSFDQAWGTIIKAD
                     VSQLIGAVILYALSVGAVKGFAFYLAVTTLLDLILAYTFIRPATLLSVRSKLGDRPAL
                     FGVSVPPTRDEVRQQGPVEGGVSA"
BASE COUNT          467 a          841 c          840 g          427 t
ORIGIN      
        1 ccggatagtc gggaaggtcc gtgatgaaac gcgagaggtc ctctggggtc atgggcgccg
       61 agcgtagcgg cacccgctgc gggtcctcgc ccaccgatcg gcggcgcggg cgccgggcac
      121 ccgcgccaca gactcagcgc ttcttgccct gcttgcgcgg acgcggcggc accgctcggt
      181 cgtacttctg tgccaactcc gccgtggtcg cggcctgatc gacggaacgc tccacagccc
      241 cgaccttcgc agtctcgcga cggcgggtgg ccccttcggg aaccacgccg acctcccaac
      301 tgtccacctt ggggatgtga tcggcctcgg cctcgatacc ccgggcacgt gcgcgcgagc
      361 gaacccggga atacgcaggc tcgcgttctt tgagccacac cagcatggga gcggcgacgc
      421 cgatggagga atacaccccg atcgccaaac cgatgatgag cgccaacgag aagtccatca
      481 tcgtcggctg accgaacatc agcccgccca cgatgaagat ggccagcggc ggaaggatcg
      541 caaccatgga ggtgttcacc gagcgcatga tcacctggtt gagcgagcgc ctcttgatgg
      601 cgttgtaggt gtacttgccg ctcgggtcgt acttggcggc gttctcccgc acccggtcgt
      661 acacgacgat cgtgtcgtac aacgagaacc cgagaatggt caggatggag atgaccgtcg
      721 ccggggtgac ctcgaaacgg gcgatggcgt agatgccgac ggtgatcaac acgtcgtggg
      781 ccaccgcgag caggcccgac accgccatgc gccactcgag ggtccaggcc atgtagccgg
      841 cgatcacgat gaagaacacg atcagcgccc gaaccgcgtc gcgggtgatc gacttacccc
      901 acgacgggcc gatcgtgttg gtgccgatgt cggtgacgtc gatctgggcg tcgtcgggag
      961 cgctggagtt cgcggcatcg gcgagggcct ggcggatctc gttcgactcc gaaatgtctt
     1021 tcgcagcagt cgcctgcacg cgaaggatcg acgtgccctc ggcgtcggtg atccgctgaa
     1081 tcttggcgcc accgagcttg tcgaacgatt cgagtacctg ctcggcctga tcgctggaga
     1141 tgtgtttgga gggaacctcc cagacggcac cacctttgaa gtcgatcgac aggttgagac
     1201 cccagatcga gatcaccacc accgacacga ccgtcgccgc cgcgctgacg agcatcatcc
     1261 agcgccacag gccggtgaag tcgaaatccg tctgaacccg atagagcttc ttacgtgtgc
     1321 tgctcatgcg gacacacctc cctccacggg accctgctgt cgcacctcgt ccctcgtcgg
     1381 tggaaccgaa acaccgaaca gcgccggccg gtcacccagc ttcgaacgca ccgacaacag
     1441 ggtcgccggg cggatgaacg tgtaggccaa gatgaggtcg agcagcgtcg tgaccgcaag
     1501 gtagaacgcg aaccccttca ccgcgccgac cgacagcgcg tagaggatga ccgctccgat
     1561 cagctggctg acgtcggcct tgatgatcgt tccccacgcc tgatcgaagc tcttttcgac
     1621 ggctcggcgg aagttgcggc cttccgacac gtcctctttg acgttttcga aatacacgat
     1681 ggaactgtcg agcgacacgc cgatcgacac gatgatgccg acgatgccgg cgagggtcag
     1741 cgccaacccc tgggtttcac cgaggaaccc gaccaccgcc cacagcatcg ccgcggtcac
     1801 caacagcgag ccgatcgtga tcaacccgag gatgcggtag tacgcgacca tgtagatgat
     1861 cacgacggcg agaccgatga gaccagcgat gatcccagcg cgaagcgcgc cttcgccgag
     1921 ggtggccgaa acgaactgca cggtctgagg ctcaagttcg atcggcaacg agccgtagcg
     1981 cagcgccagc gacaggtcgc gggcctcctg ttcggtgaac ccgccggcgg ccgagtttcc
     2041 ggaaatcgtg atctgatcgc gggtgtagct cgccgcctga atgaccgggg ccgatatcac
     2101 ctcaccgtcg agcaccacgc cgagctgccc ggcctcggcc ccgacggcgg gacaggttgg
     2161 ctggccggcg taacactgcg ccgcggcctg attgaacagg tcgatgccgt cggcgccggt
     2221 cttgaacttc ggcatcacca cccactgacc gttctgcagg ctcggggtgg catcttcgat
     2281 ggcgcgaccg gtcaacagcg tcgggccgag ttggtagata acccggttgc cgtccttgtc
     2341 gggggtgccg aagagcgtga cgggctgatc ggccttgtcg tcctcggggg ccgtgatccc
     2401 actcgaggcc gcctcgatcg agtactcgag gccgagcagt tcgaggaaat cctcgtcgct
     2461 cacgtcgatt ccgaactggt tcggaacggt cgtgggcggg ctggcggggt ccgtggtcgg
     2521 cgcctcggtc gccggatctg cggcggggtc ggctgccggg tccgcggcag ggtct
//
LOCUS       DAOOMA010000405         2567 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_49688_length_2567_cov_4.208201, whole genome shotgun sequence.
ACCESSION   DAOOMA010000405 DAOOMA010000000
VERSION     DAOOMA010000405.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 2567)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2567)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2567
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49688_length_2567_cov_4.208201"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..524
                     /locus_tag="PLP95_10320"
     CDS             <1..524
                     /locus_tag="PLP95_10320"
                     /inference="COORDINATES: protein motif:HMM:NF037527.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB46236.1"
                     /translation="VDTAIRGVLSGSTLPLILAASQPMAALFRSVNTYPHLAGERHHS
                     NPSSASDAELADGARTILDEVYADQLAELAERFDAMRSQGRTAVDLSDLARLSTLGAV
                     DTLLVDMDRSIPGSIDEAGVVTFDEADDATNHGVADEIARRVFTAGGRVLAVRSDDLP
                     DGDAAAGILRFAV"
     gene            666..1877
                     /locus_tag="PLP95_10325"
     CDS             666..1877
                     /locus_tag="PLP95_10325"
                     /inference="COORDINATES: protein motif:HMM:NF024813.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HPB46237.1"
                     /translation="MGLIQAAIGALGGTMADQWKDFYTVPEGLTPTAALFAAVPRGQN
                     AGRGSNTKGSDGVITNGSRIVVPEGYGLVLMEQGAITGFAAEPGAYEWNSEAQDSKSI
                     FAGNGIVSPLITTSWERFKFGGRPGTEQRAYFVTLKELPNNRFGTQSEIYWDDAYMNA
                     QVGAITRGTYTLKIVDPILFLKTWVPAKYLAPGEVFDFTDIENDAAGQLFNEVVGSLA
                     PAFSLYTNDPSKGNRITKIQQDSIGFAQSLSAAVEQNYQWSGRGLQIISTAIIAIEYD
                     ENTRELLKNVQRADALSGARGNSNLQASVAAGFEAAGENAGAAGLVGFGVAAGGAGLG
                     GLQQPVPGVGAQSTPPPVPPAAPAPVAAPAPAAAEEDPVAKLTKLKEMLDAGLITQDD
                     YDAAKNKVLGL"
     gene            2054..>2567
                     /locus_tag="PLP95_10330"
     CDS             2054..>2567
                     /locus_tag="PLP95_10330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB46238.1"
                     /translation="MPGVPVEDATVDTQNARLDDGVNRCPRCGSTDIQLRASAGMLMC
                     LFCRHEWAEARLDESVATGDIGSLRGTVVGSGAADIDAAADTTITLKCGGCGAEVVIN
                     TDVEMGARCHWCRHVLTINEQVPNGAVPDAVLPFTITHDKAVAIIAEFAGKRRTFADR
                     TFVREFTPENV"
BASE COUNT          448 a          851 c          839 g          429 t
ORIGIN      
        1 aggtcgacac cgccattcgc ggagtcctgt cgggttcgac ccttccgttg atccttgcgg
       61 cctcccagcc gatggccgcg ttgttccggt ctgtgaatac ctacccccac cttgcgggtg
      121 aacgtcatca cagcaacccc agttcggcct ccgacgccga gctggcggat ggtgcacgca
      181 ccatcttgga tgaggtgtac gcagatcaac tcgctgaact cgctgaacgg tttgacgcga
      241 tgcgttcgca aggccgcacc gcggtcgacc tgtcggactt ggctcgactc tcgacattgg
      301 gagcggtgga cacactgctc gttgacatgg atcggtcgat tccgggctcc atcgacgaag
      361 ccggtgttgt cacctttgac gaggccgacg acgccaccaa ccacggtgtc gccgacgaga
      421 tcgcccggcg ggtattcacc gctggtggtc gggtgctcgc cgttcgctcg gacgacctgc
      481 ccgacggcga cgcggccgcg ggaatccttc gtttcgcggt ctagccacgg cactggtggc
      541 ttgccggtcg ggtggctgtc cgcggatggc tacccgaccg atcacgccca agttggcgtc
      601 gaaattcgac gacggctcga taggctcacg ttgtcatccg cgaccaacac aagagggatc
      661 tgaccatggg gcttattcag gcagcaatcg gtgcgctcgg cggcacgatg gccgatcagt
      721 ggaaggactt ctacaccgtt cccgaggggc tgacgccgac cgccgccctt ttcgccgcgg
      781 tgccgcgtgg ccaaaacgcg ggccggggct ccaacacgaa gggttccgac ggggtcatca
      841 ccaacggcag ccgaatcgtc gtgcccgagg ggtacggcct ggtgctcatg gagcagggcg
      901 cgatcaccgg ctttgctgcc gaacccggtg cctacgaatg gaacagcgag gcgcaggact
      961 cgaagtcgat ctttgccggc aacggcatcg tgagcccgct gatcaccacg tcgtgggagc
     1021 gattcaagtt cggtggccgt ccgggcaccg aacagcgcgc gtatttcgtc accctcaagg
     1081 aacttccgaa caaccgcttc ggcactcaat cggagatcta ctgggacgac gcctacatga
     1141 acgcccaggt gggcgcgatc acccgcggca cctacaccct caagatcgtc gatccgatcc
     1201 tgttcctcaa gacctgggtg ccggccaagt acctcgcccc gggtgaggtg ttcgatttca
     1261 ccgatatcga aaacgacgcc gccggacagc ttttcaatga ggtcgtcggg tcgctggccc
     1321 cagcgttctc gctgtacaca aacgacccgt cgaaggggaa ccggatcacc aagatccagc
     1381 aggattcgat cgggttcgcc cagagcctct ccgcggcagt cgagcagaac taccagtgga
     1441 gcgggcgcgg cctgcagatc atctccaccg cgatcatcgc gatcgaatac gacgagaaca
     1501 cccgtgaact gctcaagaac gtgcagcgtg ccgacgcgct gtccggtgcc cgcggcaatt
     1561 cgaacctgca ggcctcggtc gccgctggtt tcgaagcggc cggcgagaat gccggagcgg
     1621 caggcctggt cgggttcggc gttgccgcag gcggagcggg tctcggcgga ctgcaacagc
     1681 cggtgcccgg agtcggggcc caaagcacac cgcctccggt gccgccggcg gcgcccgctc
     1741 ccgtagcggc tccggcccct gcggcggctg aggaggatcc ggtggccaag ttgacgaagc
     1801 tcaaagagat gctcgatgcc ggcctgatca ctcaggacga ctacgacgcc gccaagaaca
     1861 aggtgctcgg gctgtaggcc ctcaggcctc gcagtctccg ccgccctcga cggtcgcgca
     1921 cgaaatgtca tcgatgcccc ctccggaggg gaccgcgggt caacttggcg ctcagcccac
     1981 gaccgcgggt ccccctccct atgcgccgcc ggtgccaccg gcagttccga acgcgccgct
     2041 cgagataccc gggatgcccg gcgtgccggt tgaggacgcc acggtcgaca cgcagaacgc
     2101 gcgactcgac gatggtgtga accggtgccc gcgctgcggc tcgaccgaca tccagctgcg
     2161 ggcgtcggcc ggcatgctca tgtgtctgtt ctgtcgacat gagtgggccg aggcgcgcct
     2221 agacgagtcg gttgcgaccg gcgatatcgg ctcgttgcgc ggcaccgtcg tggggtctgg
     2281 cgccgcggac atcgacgccg ccgcggacac gacgatcacg ttgaagtgcg gtggttgcgg
     2341 cgcggaggtg gtgatcaaca ccgacgtcga gatgggcgcg cggtgtcact ggtgccgcca
     2401 cgtgctcacc atcaacgagc aggtacccaa cggcgcggtg cccgacgcgg tgttgccgtt
     2461 cacgatcacc cacgacaagg cggttgcgat catcgccgag ttcgcaggga aacgccggac
     2521 ctttgcggac cggaccttcg tgcgggagtt cacccccgaa aacgtgg
//
LOCUS       DAOOMA010000406         2567 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_49689_length_2567_cov_3.894506, whole genome shotgun sequence.
ACCESSION   DAOOMA010000406 DAOOMA010000000
VERSION     DAOOMA010000406.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 2567)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2567)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2567
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49689_length_2567_cov_3.894506"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..981
                     /locus_tag="PLP95_10335"
     CDS             <1..981
                     /locus_tag="PLP95_10335"
                     /inference="COORDINATES: protein motif:HMM:NF023326.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3418 domain-containing protein"
                     /protein_id="HPB46239.1"
                     /translation="SFRIVDSDGVELAAGKDLRVLAHEVRQHADEVATQVDSGLERSG
                     IRTWDLGELPRVVQRHVGGRLLKAYPALADDGDSVAVVACPSAEEQAAMMWEGTRRLL
                     RLQMPSPARQVDSLLDERTKSLMTTGAIASKVEWYNDIIATTLDRLLADAGGPAWNEP
                     AWARLVADARNGFHDALVLSCAAASRIIAAHRDIRAAMASSVSEAVDPAMVDIAEQLD
                     RLLYPGVLTAVGYDRLDDIERYLRAVLRRLDALPNNVRRDAELMQRCRALESDYDSLL
                     ATVPMSDDVEAVGWMLEEFRVSSFAQQLRTSVPVSEKRIRNEIHRLARGL"
     gene            complement(996..1964)
                     /locus_tag="PLP95_10340"
     CDS             complement(996..1964)
                     /locus_tag="PLP95_10340"
                     /inference="COORDINATES: protein motif:HMM:TIGR00968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter ATP-binding protein"
                     /protein_id="HPB46240.1"
                     /translation="MTISARNINKKFGDFVALDDVSVDIGGGNLTALLGPSGGGKTTL
                     LRIIAGLDDPDSGVVEINGADTTWVRPQQRNVGFVFQHYAAFKHLSVFRNVAFGLEIR
                     KTSKEEIRKRVMELLELVHLDQFADRLPAQLSGGQRQRMALARALAVEPSVLLLDEPF
                     GALDAKVRKELRDWLRRLHDEVHVTTVFVTHDQEEALEVSNEIVVINQGRVEQVGSPD
                     DLYDHPRNDFVMGFLGPTTRLNGELVRPHDIAVTTSAVPHAFGARVTRLLRVGFEVRL
                     DATREVIGEPDETVTVQLTRREAVDLDLREGDEVYLSHAFSQVLVG"
     gene            complement(1972..>2567)
                     /locus_tag="PLP95_10345"
     CDS             complement(1972..>2567)
                     /locus_tag="PLP95_10345"
                     /inference="COORDINATES: protein motif:HMM:TIGR00969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sulfate ABC transporter permease subunit"
                     /protein_id="HPB46241.1"
                     /translation="FGIAVALLLVRYRFPGRRLLSTFVDLPLSVSPIVAGLALIFAYG
                     RNGWFGPALDAAGIDVIFAFPGIVLATVFVSMPLVVREIVPVLEEIGTEQEQAATVLG
                     ASWFQTFRRITLPSIKWAVAYGVVLALARSLGEFGAVKVVTAGGVAGSTQSVTSLVEE
                     RYAEFDELSAYSASLVLAAATILCLIVITIIRPSHQK"
BASE COUNT          494 a          847 c          813 g          413 t
ORIGIN      
        1 tcgttccgca tcgtcgacag cgacggcgtc gaactcgccg caggcaagga tcttcgagtg
       61 ctcgcgcatg aggtgcgcca acatgccgac gaggtggcaa cccaggtgga ctccggcctc
      121 gagcggtccg gcattcggac gtgggacctg ggcgaactcc cacgggtggt gcagcgtcac
      181 gtgggtggcc gcctcctcaa ggcctacccc gcgctcgcgg atgacggcga ctcggtggcg
      241 gtggtcgcct gccccagcgc ggaagagcag gcagcgatga tgtgggaagg cacgcgccga
      301 ctgcttcgac tacagatgcc cagcccggca cgacaggtcg actccctgct ggacgaacgc
      361 acgaagtcgc tcatgacgac cggggccatc gcgtcgaaag tcgagtggta caacgacatc
      421 atcgcaacga ccctcgaccg tctcctcgca gacgctggcg gaccggcctg gaacgagccg
      481 gcctgggcgc gcctcgtcgc ggatgcccgc aacggatttc acgacgcgct cgtgctgagc
      541 tgcgccgctg cttcgaggat cattgcagcg catcgcgaca ttcgcgctgc catggcctcc
      601 tcggtctcgg aggcggtcga cccagccatg gtcgacatcg ccgagcaact cgatcgactc
      661 ctgtatccgg gtgtgctcac ggcggtgggg tacgaccggc tcgacgatat cgaacggtac
      721 cttcgcgccg ttctgcggcg actcgacgct ctccccaaca atgtgcgccg cgacgctgag
      781 ctgatgcaac ggtgtcgggc gctcgaatcc gactacgaca gcctcctcgc cacggtaccg
      841 atgagcgacg acgtcgaggc ggtcggctgg atgctcgagg agttccgtgt cagctcgttc
      901 gcccagcaac tccgaaccag cgtcccggtg agcgagaagc gcatccgaaa cgagattcac
      961 cgcctggcgc gagggttgtg agcgcccgtt ggccctcagc cgacgagaac ctgcgagaac
     1021 gcatgagaca agtacacctc gtcgccctcg cgcagatcga gatcgacggc ctcgcgtcgt
     1081 gtgagctgaa ccgttacggt ctcgtctggc tccccgatca cctcgcgcgt cgcatcgagg
     1141 cgaacttcga aaccgacccg tagcagccgg gtcacccgag cgccgaacgc gtgcggtacc
     1201 gctgaggtgg tgaccgcaat gtcgtggggt cgaaccagct caccgttgag gcgcgttgtc
     1261 gggccgagaa atcccatcac aaagtcgttg cgagggtggt cgtagagatc gtcgggcgag
     1321 ccgacctgtt caacgcgacc ctgattgatg accacgattt cattcgacac ttccagtgcc
     1381 tcttcctggt cgtgggtcac gaacaccgtg gtcacgtgca cctcgtcgtg gagccgtcga
     1441 agccagtcgc gcaactcctt tcgaaccttc gcatcgagcg cgccgaacgg ctcatcgagc
     1501 aacaacacgc tcggttccac cgccaacgcc cgggcaaggg ccattcgttg gcgttgccca
     1561 cccgaaagct gcgccggaag ccgatcggcg aactggtcga ggtgaacgag ttcgagcaac
     1621 tccatcaccc gtttgcggat ctcctccttg gaggtcttac gaatctccag accgaaggcg
     1681 acgttgcgaa acacgctcag gtgcttgaac gccgcatagt gctgaaacac gaagccgacg
     1741 tttcgctgtt ggggacgaac ccaggtggtg tccgctccgt tgatctcgac cacccccgag
     1801 tcgggatcgt cgaggccggc gatgatgcgc aacaaggtcg tcttgcctcc accggacggg
     1861 ccgagcagag cggtcaggtt gccgccaccg atatcgaccg agacgtcgtc caacgccacg
     1921 aagtcgccga atttcttgtt gatgttgcga gcggagatgg tcatggcgat gtcacttctg
     1981 gtgcgaaggg cgaatgatgg tgatgacgat caggcacagg atcgtcgcgg ccgcgagtac
     2041 caacgaggcg ctgtacgcgc tgagttcgtc gaactcggca tagcgctcct ccaccaagga
     2101 ggtcacgctc tgggtagacc cggccactcc cccggcggtg acgaccttga ccgccccgaa
     2161 ctcaccgagc gaacgggcga gcgccaacac caccccgtac gccaccgccc atttgatgga
     2221 cggcagggtg atgcgtcgga aggtctggaa ccacgatgcg cccaacacgg tcgctgcctg
     2281 ttcctgctcg gtgccgatct cctcgagcac gggcacgatc tcccggacga cgagcggcat
     2341 cgagacgaac accgtggcca acacgattcc cgggaaggcg aagatcacgt cgatgccagc
     2401 agcgtcgagg gccggaccga accagccgtt gcgcccgtac gcgaagatca gcgccagacc
     2461 ggcgacgatc ggcgacaccg acagcggcag gtcgacaaac gtgctgagca gacgccgtcc
     2521 ggggaaccgg taccgcacca acaagagcgc aacggcgatg ccgaaca
//
LOCUS       DAOOMA010000407         2564 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_49792_length_2564_cov_3.285771, whole genome shotgun sequence.
ACCESSION   DAOOMA010000407 DAOOMA010000000
VERSION     DAOOMA010000407.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 2564)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2564)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2564
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49792_length_2564_cov_3.285771"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..330
                     /locus_tag="PLP95_10350"
     CDS             <1..330
                     /locus_tag="PLP95_10350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB46242.1"
                     /translation="FSADLAPDLLAPWRHPTTTITYAADRLPVAEAGFVPAEGRADAS
                     LILRWSDDHRLLSPAPPWLDEVDGFPVTDPCQQWWDLLDLGGEDRAEAADRLRSAILG
                     RTLPRPR"
     gene            complement(694..1629)
                     /locus_tag="PLP95_10355"
     CDS             complement(694..1629)
                     /locus_tag="PLP95_10355"
                     /inference="COORDINATES: protein motif:HMM:NF014754.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004371 - glycerone kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HPB46243.1"
                     /translation="MEPLRQLRSDDVSEFLRAAAVSARAHAPGIDHLDHLDQLEGIEG
                     GNAGSNVAATLEALVARLTAGETLTGLASLLEGARAGAVGTAGNMVAEFLAGLGELCR
                     NTDALDPTRLAMGFEVGVERLVDVVGEERPGGIPSVATVAARTALRLADEGIGMADLV
                     LAVAEDALDALEATTDLWPPLAEAGVVDAGAAAFVVVLDALVATVHGEDPEVPEWEFV
                     ENLDPELSESERFRYVVSLRLAVPERVGTPRSAEEMLAHLTAAWRALGDEVTTGFADD
                     GAVWASIATDDIGPVIEAAIGVGRPYNIAVDDTRG"
     gene            1796..2002
                     /gene="rpmB"
                     /locus_tag="PLP95_10360"
     CDS             1796..2002
                     /gene="rpmB"
                     /locus_tag="PLP95_10360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015798920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HPB46244.1"
                     /translation="MSSVCDICAKKPGFGMSLSHSHRRNKRRWNPNIQRVRAVVNGSP
                     KRVNVCTGCLKSGKVQKPVARQIP"
     gene            2012..2275
                     /locus_tag="PLP95_10365"
     CDS             2012..2275
                     /locus_tag="PLP95_10365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB46245.1"
                     /translation="MQGVIKSYDPGTDDGTIVGDTDFVSYDLAPGAVAAAGLRFVRQG
                     QRLLFTVNDDGLAADLRFGSEVDMGTPGFFESLPSAEPVADET"
     gene            2439..>2564
                     /locus_tag="PLP95_10370"
     CDS             2439..>2564
                     /locus_tag="PLP95_10370"
                     /inference="COORDINATES: protein motif:HMM:NF036305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB46246.1"
                     /translation="MPGTTNNQKLLDWVEHWRSIFEPDDVYWCDGTAEEYDRMCQE"
BASE COUNT          415 a          945 c          811 g          393 t
ORIGIN      
        1 ttctccgctg atctcgcccc agacctgctc gccccgtggc gtcaccccac gacgacgatc
       61 acctacgcag cagaccgcct gcccgtagcc gaggccgggt tcgtccccgc cgaaggccgt
      121 gccgacgcca gcctcatctt gcgatggtcc gacgaccatc gactcctctc ccctgcccca
      181 ccctggttgg acgaggtcga cggctttccg gtcaccgacc cgtgccagca atggtgggac
      241 ctgctcgacc tcggcggcga agaccgagcc gaagccgccg accgcctccg ttcggccatc
      301 ctcgggcgaa cgttgccgag gccccgatga ccggcggacg tgtcgtgctc gcgagcctgt
      361 cggccgccat ggacggcggg ttcaccgcgg ttgccgacgt gtcgtcggcc atggcggcca
      421 ccggcatgac cgagcaccat cgcctcatcg cccggcgcca tctcgcgaat cgccgcttgg
      481 cgggtccggt cgaggtgtcg gcgcgggtag tgcatcaaca gatccaacag cgaccggaac
      541 ccgctggcgt gcaacgcctg cagcttcttc ggcccgacac gagccaggcg gtcgaccggc
      601 atcgccgcca ggtcgtgcag cgtgatcggc ggggcagccg cgtgggcggt cggcgcagcg
      661 gccgccgggg cggtcggcgg ggtggtcggg cgctcagcca cgcgtgtcgt cgaccgcgat
      721 gttgtagggg cgaccgaccc cgatcgccgc ctcgatgacg gggccgatgt cgtcggtggc
      781 gatcgacgcc cacactgcgc cgtcgtcggc gaacccggtg gtgacctcgt cgcccagcgc
      841 ccgccacgcc gcggtcaggt gcgccaacat ctcctcggca gaacgcggcg tcccgacgcg
      901 ctccggcacc gccaaacgaa ggctcaccac gtagcggaac cgctccgatt ccgacagctc
      961 agggtcgagg ttctccacga actcccactc cggaacctcg gggtcttcgc cgtgcaccgt
     1021 cgccaccagt gcgtccaaca ccacgacgaa cgccgccgct ccagcgtcga cgacacccgc
     1081 ctcggcgagc gggggccaca gatcggtggt ggcctcgagt gcgtcgagcg catcctcggc
     1141 gactgcgagc accagatccg ccatgccgat gccctcatcg gcgaggcgaa gcgccgtgcg
     1201 cgccgcaacc gtcgcaacgc tcgggattcc tcctggccgc tcctcgccga cgacgtcgac
     1261 gagccgttcg accccgacct cgaatcccat ggccaggcgg gtgggatcga gcgcgtcggt
     1321 atttcggcac agctcgccca gcccggcgag aaactccgcg accatgttgc cggccgtccc
     1381 gacggcgccc gcccgcgctc cctcgagcag cgaggccaac ccggtcaggg tctctcccgc
     1441 ggtcagccgg gccacgagtg cctccagcgt ggcggccacg ttcgagccgg cgttgccacc
     1501 ctcgatgccc tcgagttggt ccaggtgatc gaggtgatcg atccccgggg catgggcgcg
     1561 ggccgacacc gcagcggccc gaagaaactc cgagacgtcg tcggatcgaa gttggcggag
     1621 cggttccatc tcgacgaacc tatccgagcg ttgaggcccg tggcttcgtg cccacccggc
     1681 aacggtgcta acgtctgcgg agcgttcgga ctctcgcgcc ggcgcaccac tcgacgcatc
     1741 cgaccgaccc ggcgggcgca tcatcccctc atttcgtgaa gatcaggaat ccaccatgtc
     1801 ctccgtctgc gacatctgcg caaagaagcc cggcttcggc atgagcctca gccactccca
     1861 ccgccgcaac aagcgccgct ggaatccgaa cattcagcgc gtgcgcgccg tggtgaacgg
     1921 ctcacccaag cgggtcaacg tgtgcaccgg ctgcctcaag tccggcaagg tgcaaaagcc
     1981 cgtcgcccgc cagattccct gagtcactgc gatgcagggc gtcatcaagt cctacgatcc
     2041 cggcaccgac gacgggacga tcgtgggcga caccgacttc gtttcctatg acctcgcacc
     2101 gggcgctgtc gccgctgccg gcctgcgttt cgttcgccag gggcagcgcc tgttgttcac
     2161 cgtgaatgac gacggcctcg ccgccgacct gcgtttcggg tccgaggtcg acatgggcac
     2221 gcccgggttc ttcgagtcgc tgccgagcgc tgagcccgtc gccgacgaga cctgacaatc
     2281 gatcaccgaa acggggccct acggccctgt tttctttttc ccgcccggtt cgacgggtca
     2341 tgtgaccttt cccactgggc ccaaatgcgg gttgcacaga cgtgctctcc gcggtaagac
     2401 caacgaacga catcgccacc agcgaatccg gaggaaacat gccaggcacg accaataacc
     2461 agaagctgct cgactgggtc gagcactggc gatccatctt cgaaccggat gacgtctact
     2521 ggtgcgatgg gaccgccgag gaatacgacc ggatgtgtca ggaa
//
LOCUS       DAOOMA010000408         2561 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_49874_length_2561_cov_3.615323, whole genome shotgun sequence.
ACCESSION   DAOOMA010000408 DAOOMA010000000
VERSION     DAOOMA010000408.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 2561)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2561)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2561
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49874_length_2561_cov_3.615323"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            229..1008
                     /locus_tag="PLP95_10375"
     CDS             229..1008
                     /locus_tag="PLP95_10375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020376892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HPB46247.1"
                     /translation="MPGSVRLEIDGGVAVITNDNTEKHNAFDDAMDAQLFGILEQLSD
                     DPSVRAVVWRGEGKSWSSGRDTSIIGNASTDLTHHQMMVRGHRGIQRLWELDAPVIVA
                     IQGWAIGGSFQRALLCDIRICTPDAKFMLPEVGHGVIPDTGGMGVLYEMCGHGLVSDM
                     VLTGRRLSADEALQHGIVSRIVEADELDATAMEMAQKIAASPAVTVKLARRVISHLSR
                     PQIVTSMDDELIYQTFLNRSDDFAEFKAARAEGREPRYTGS"
     gene            1010..1915
                     /locus_tag="PLP95_10380"
     CDS             1010..1915
                     /locus_tag="PLP95_10380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020376893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HPB46248.1"
                     /translation="MVDRSQFPGIPVPPAVGASAMAPGTYDGETVFVTGGGTGLGKAI
                     ASEFARLGANIVIASRKPEHLAAGIEAIEAIGAEVIGVECDIREPDSIAAAFDAAGQR
                     FALPGVLINNAAANFPVPAEDMSPNAWRTVVDITLNGTFFVSREFGRRHIEAGTPASI
                     LSVGASYAWTGGPGFAHSSAAKAGVKNLTETLAVEWGPYGISVNCLVPGLFPHEDMTA
                     DITGNLDRTTDKDACQPALRVGHRQELGWAATFLASPFARFISGHTLVVDGANWQRRM
                     LTNPPVVPNREQMGRGSFDPNPPAR"
     gene            complement(1942..>2561)
                     /locus_tag="PLP95_10385"
     CDS             complement(1942..>2561)
                     /locus_tag="PLP95_10385"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HPB46249.1"
                     /translation="RVVAVPTDITKPDECQRIVDTAIERFGRVDGLAQVAAYTGASGG
                     LRAADFDKVRRSIETNLIGSMRISTAAADAMRETGGGSIVLVGSQSMLAPLIEQIGYA
                     ASKGALLSASFHMAKELGPDNVRVNTVVPNWMWGPPVETYVQWQATANDITETEVKAK
                     LEAAIPLGEIVPDEEVANAIAFFLSDRARCITGQTLLVNGGEMMR"
BASE COUNT          435 a          845 c          841 g          440 t
ORIGIN      
        1 gttgcgccgc gggggagtgc tcgtcgtggt cggcaatcgg cacctgaact accacaacaa
       61 gttgaggcgg ctgttcggca acgtcgacac gatcggctcc tcaaagaaat tcgtcgtgtt
      121 ggcggcgcac aagcgctgag gacgttcggc gggccggtgt cgacaaccgt gccaacgcct
      181 aacatcggtc acacctcgcg cccggagatg tgcggggagg gagacgagat gcccggaagc
      241 gtacggctcg aaatcgacgg cggtgtcgcc gtcatcacca acgacaacac cgagaaacac
      301 aatgcgttcg atgacgcgat ggatgcccaa ctgttcggga ttctcgagca gctttcggac
      361 gacccgtcgg tgcgcgccgt ggtgtggcgc ggcgagggca agtcgtggtc ctcggggcgc
      421 gacacctcga tcatcggcaa tgccagcacc gacctgaccc atcatcagat gatggttcgc
      481 ggtcaccgcg gtatccaacg gctctgggaa ctcgacgccc cggtcatcgt ggcgattcag
      541 gggtgggcga tcggcgggtc gttccaacgg gcgttgctgt gtgacattcg catctgcact
      601 cccgacgcga agttcatgtt gccagaggtc ggccacggcg tgatccccga caccggtggc
      661 atgggtgtgc tctatgaaat gtgcgggcac ggactcgtca gcgacatggt gctcaccggc
      721 cgacggctga gcgccgacga ggcgctgcaa cacgggatcg tgtcgcgcat cgtcgaagcg
      781 gacgaactcg acgcgaccgc gatggagatg gcgcagaaga ttgcggcctc gccggcggtc
      841 acggtgaagc tcgcccgtcg ggtcatctcg catttgtcgc gcccccagat cgtgacgtcg
      901 atggacgacg agttgatcta ccagaccttc ctcaatcgat ccgacgactt cgccgagttc
      961 aaggcggcac gagccgaggg gcgcgagccc cgctataccg gaagctgaca tggtcgaccg
     1021 ttctcagttc cccggcatcc cggttccccc cgccgttgga gcgtcggcga tggcgcccgg
     1081 aacctacgac ggtgaaacgg tgttcgtcac cggcggcggc accggcctcg gcaaggcgat
     1141 cgcctcggag ttcgcccgct tgggcgcgaa catcgtcatc gcctcgcgca agcccgagca
     1201 cctggcggcc ggcatcgaag cgatcgaggc catcggcgcc gaggtgatcg gcgtcgagtg
     1261 cgatattcgg gagccagact cgatcgccgc agccttcgac gccgccggac agcggtttgc
     1321 gctgcccggt gtgctgatca acaacgcggc cgccaacttc cccgtaccgg ccgaggacat
     1381 gtcgccgaac gcctggcgca cggtcgtcga catcacgttg aacggcacat tttttgtgag
     1441 ccgcgagttc ggtcggcgac acatcgaggc cgggaccccc gcttcgatcc tcagcgtcgg
     1501 agcgtcctac gcctggaccg gcggccccgg attcgctcac agctctgccg ccaaggccgg
     1561 ggtcaagaac ctcaccgaaa ccctggcggt cgagtggggt ccttatggca tttcggtcaa
     1621 ctgcctcgtg cccgggctgt ttccgcacga ggacatgacc gcggatatca ccggcaacct
     1681 cgaccgcacc accgacaagg acgcctgcca gccggcgtta cgcgttggac atcgccagga
     1741 actcgggtgg gccgcaacgt ttctggcgtc acccttcgcc cgtttcatca gcggccatac
     1801 cctggtcgtg gatggcgcga actggcagcg gcgcatgctc accaacccgc cggtcgtgcc
     1861 gaaccgtgag cagatgggtc gaggctcatt cgaccccaac ccgccagccc ggtagcggcc
     1921 gggttctgaa cgcggcgagc atcagcgcat catctcgccg ccgttgacca acagcgtctg
     1981 tccggtgatg caacgtgcgc ggtccgacag gaagaaggcg atcgcgttcg cgacctcctc
     2041 gtcgggcacg atctcgccga gggggatggc ggcttcgagc ttggccttca cctcggtctc
     2101 ggtgatgtcg ttcgccgtgg cctgccattg cacgtaggtc tccaccggcg gcccccacat
     2161 ccagttcggc acgacggtgt tcacgcgaac gttgtcgggg ccgagttcct tggccatgtg
     2221 gaagctggca ctcaacagtg cgcccttgga cgccgcgtac ccgatctgtt cgatgagcgg
     2281 tgccagcatc gactggctgc cgacgagcac gatcgacccg ccgccggtct cgcgcatggc
     2341 atccgcggcg gcggtcgaga tccgcatcga gccgatgagg ttggtctcga tgctgcgtcg
     2401 aaccttgtcg aagtcggcgg ctctcaggcc acccgacgct ccggtgtagg cggcgacctg
     2461 cgccaggccg tcgacgcgac cgaaacgctc gatcgccgtg tcgacgattc gctggcactc
     2521 gtcgggcttg gtgatatcgg tcggcacggc aacgacgcgc t
//
LOCUS       DAOOMA010000409         2557 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_49979_length_2557_cov_3.932054, whole genome shotgun sequence.
ACCESSION   DAOOMA010000409 DAOOMA010000000
VERSION     DAOOMA010000409.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 2557)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2557)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2557
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49979_length_2557_cov_3.932054"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..595
                     /locus_tag="PLP95_10390"
     CDS             <1..595
                     /locus_tag="PLP95_10390"
                     /inference="COORDINATES: protein motif:HMM:NF016952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016603 - glutaminyl-peptide
                     cyclotransferase activity [Evidence IEA];
                     GO_process: GO:0017186 - peptidyl-pyroglutamic acid
                     biosynthetic process, using glutaminyl-peptide
                     cyclotransferase [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glutaminyl-peptide cyclotransferase"
                     /protein_id="HPB46250.1"
                     /translation="GRSQLRRIVPETGEVEVVQNLPESVFGEGLAIGPEGFVQLTWQQ
                     RRAYRWSHDARDDLGTFTYDGEGWGLTYDGTQFIQSDGSATLTFRDPSDFTVVKTLPV
                     TIDGEPLDQLNELEWVDEMVWANVWHSDTIVGIDPGSGEVAATVDASGLWADSARTQE
                     MTLNGIAHRPGDPPTRLWLTGKLWPTLFVVDLVEVQP"
     gene            592..1077
                     /locus_tag="PLP95_10395"
     CDS             592..1077
                     /locus_tag="PLP95_10395"
                     /inference="COORDINATES: protein motif:HMM:NF025215.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation family protein"
                     /protein_id="HPB46251.1"
                     /translation="MTVEVDRSSGRRGRSRVALGSIAAGLLIVSMGCGADYRSLSPGE
                     CLPSSAKVVGRREADPPTTSCEGPHRYEVFAVGRLTGDEFPGQEQLDADSRMLCYEAF
                     EPSVGFPAAEMGDEIRVVYLAPTEQSWTRDDDREVECLLIFTEDREGRVATPLKGGDS
                     G"
     gene            1181..>2557
                     /locus_tag="PLP95_10400"
     CDS             1181..>2557
                     /locus_tag="PLP95_10400"
                     /inference="COORDINATES: protein motif:HMM:NF023835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SNF2 helicase-associated domain-containing
                     protein"
                     /protein_id="HPB46252.1"
                     /translation="MTNEWNWNNQVTFVPADPPRAGSLVLWDERDTGGLFAASSSERL
                     AIRRGSRIDVEAQPVARVPLGAALEALLDVDTPMSESAQTYRRATRFALELIERGRFV
                     PAVSTGGYDAWQIDPFDPLDHAVLADLAASAPPQAFALAAEIPLDGSNNTAAMVDPAW
                     LLRAYLDAVVDRFVRSAAAGEVAGSHLWAALDAAPAEPARPWTTWAYGHLISSTRLGL
                     RVEALDIVSDGLIEGDAAGQGALDDGHLTRAQREELARFKIIPQLRSTREGSLVVDMT
                     RLGRLSDPVRRRFGAATELESLVAIGRAATMWAPLEPATGTEPGEPIEVDLDGLLEFI
                     EVAEDLEAAGIEVLWPASFDADFTPSLVGTATAAPRGADDTATLGLSTLLKFHWRVFC
                     DGERLTDRELEVLADAKRSVVFLRGRWVRTDPALVERLRTVPDAVVGAGAVAGAMAGS
                     TVDDDGEVI"
BASE COUNT          406 a          795 c          923 g          433 t
ORIGIN      
        1 cgggcggtcg cagttgcgtc ggatcgttcc ggaaacaggc gaggtcgagg tggtgcagaa
       61 cctgccggag tcggtcttcg gcgaagggtt ggcgatcggc cccgagggat tcgtgcagct
      121 cacctggcaa cagcgccggg catatcgatg gagccacgac gcccgcgacg acctcggtac
      181 gttcacctac gacggcgaag gatgggggtt gacctacgac ggaacgcagt tcatccagag
      241 cgacggatca gcgacactga catttcgcga cccgagcgat ttcacggtcg tcaagacgct
      301 gcctgtcacc atcgacggcg agccgctcga tcagttgaac gaactcgagt gggtcgacga
      361 gatggtgtgg gccaacgtgt ggcattccga cacgatcgtg gggatcgacc cgggcagcgg
      421 ggaggtcgca gcgaccgtcg acgcatccgg cctgtgggcc gattcggctc ggacgcagga
      481 gatgaccctg aacggcatcg cccatcgacc gggagatccg ccgacgcgcc tgtggctgac
      541 gggcaagctg tggccgacgt tgttcgtcgt cgatcttgtg gaggtgcagc catgaccgtc
      601 gaggtcgatc gttcgagtgg gcgtcggggc cgttctcggg ttgccctcgg gtccatcgcc
      661 gcaggtctgt tgatcgtatc gatgggatgt ggggccgatt accggtcgct gtcgccgggc
      721 gaatgcctac cctcgagcgc caaggtggtg gggcgtcgag aggccgaccc gccgacgacg
      781 agctgtgagg ggccgcatcg atacgaggtg ttcgccgtcg gccgtctcac cggcgacgag
      841 tttcccgggc aggaacagct cgacgccgat tcgcgcatgt tgtgttacga ggcgttcgag
      901 cccagtgtcg gctttccggc ggcggagatg ggcgacgaga ttcgcgtggt gtatctggcg
      961 ccgaccgagc agagttggac ccgagacgac gatcgcgagg tcgagtgcct gctgatcttc
     1021 accgaggatc gcgaggggcg ggttgcgacg ccgctcaagg gtggcgacag cggctgaacg
     1081 ggctgaaagc gggcccagcg cgaagcccag cgccagactc ggaacacgtg ttcgaaatta
     1141 tccgaacacg tgttcgttaa acatgctaag gtctcgaccc gtgacgaacg aatggaactg
     1201 gaacaaccag gtcaccttcg tgccggccga cccgccccga gcaggttcgc tggtgttgtg
     1261 ggatgaacgt gataccggcg ggctgttcgc cgcgtcatcc agcgaacggt tggcgatccg
     1321 gcgcggttcg cgcatcgatg tcgaagctca gccggtggcg cgggttccgc tcggtgctgc
     1381 cctcgaggcg ctgctcgacg tcgatacccc gatgtctgaa tcggctcaga cctatcggcg
     1441 tgccaccagg ttcgcgctcg agttgatcga acggggccgt ttcgttccgg cggtgagcac
     1501 cggcggctac gacgcctggc agatcgaccc attcgaccca ctcgaccatg cggtgctggc
     1561 cgatctggcg gcatctgccc cgccgcaggc gttcgccctc gctgcagaga ttccactcga
     1621 cgggtcgaac aacacggcgg cgatggtcga cccggcctgg ctgttgcggg cctacctcga
     1681 tgcggtggtc gatcggtttg tgcgttccgc ggcagcgggc gaggtggcag ggtcgcactt
     1741 gtgggcggcg ctcgatgctg cgccggccga acccgctcgg ccatggacga cgtgggccta
     1801 cgggcatctg atctcgtcga ccaggctcgg gctacgcgtc gaggcgctcg acatcgtctc
     1861 cgacgggctc atcgaaggag atgccgcggg ccagggggcg ctcgacgacg gtcacctgac
     1921 ccgggcccag cgcgaggaac tggctcgctt caaaatcatc ccgcagttgc gcagcacgcg
     1981 ggaggggtcg ctggtggtcg acatgacgag gctcggtcgt ctgtcggacc cggtgcgccg
     2041 tcggttcggg gcggcgaccg aactcgaatc gctcgtcgcg atcggacgag cggcgacgat
     2101 gtgggctcct ctcgagccgg cgaccggaac cgaacccggc gagcccatcg aggtcgacct
     2161 cgacgggctg ttggagttca tcgaggtcgc cgaggacctc gaggccgccg ggatcgaggt
     2221 cctgtggccc gccagtttcg acgccgattt caccccgagc ctcgtgggca cggccaccgc
     2281 ggcgccgcgc ggagcagacg acaccgcgac gctggggctg tcgacgttgt tgaagttcca
     2341 ctggagagtg ttctgcgacg gcgagcgact caccgaccgc gaactcgagg tcctcgcgga
     2401 tgccaaacgg tccgtcgtgt tcctgcgcgg ccggtgggtt cgcaccgacc ccgccctggt
     2461 cgagcggttg cgcacggttc ccgacgccgt cgtgggggcc ggagcggtcg ccggggccat
     2521 ggccgggtcg accgtcgacg acgacggcga ggtcatc
//
LOCUS       DAOOMA010000410         2555 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_50028_length_2555_cov_5.242000, whole genome shotgun sequence.
ACCESSION   DAOOMA010000410 DAOOMA010000000
VERSION     DAOOMA010000410.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 2555)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2555)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2555
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50028_length_2555_cov_5.242000"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1242)
                     /locus_tag="PLP95_10405"
     CDS             complement(<1..1242)
                     /locus_tag="PLP95_10405"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HPB46253.1"
                     /translation="MTRDVSVETDWIDPFWQRLSQRVHDPVFILDRAGTCLSVSNVVS
                     AETGYSVDELVGRPFAELASESERERVARCLSESGSSGGLSEPVEISMTMCSAVSETF
                     EVQLSIWADVLDGRSVIVVIMQRSTVARSAERLLRRKESLETLITRFQYRAAHTVADD
                     VDDIIESGLGEAAIFLGADRGYVVSFDAAAEFETMTHEWTAWGIPPQRHEYVSVPLSL
                     TPLAASAQSALEVLAVRDVSKLPPEWSIDREFLEELGISSFLELPIVIGGRAVAAMGF
                     EWHTRTGDWADSDLILLGTVAATCAQLLGSAEAEDEMRRRSRHDELTGLLNRTGLVAE
                     LNEMLASAAQPGRVGVLVVDVDGFNALNDSLGSTAGDLVLKRIGARLRGLIRPGDAVA
                     RIASDEFAIGVGECQSDWALYE"
     gene            complement(1402..2364)
                     /locus_tag="PLP95_10410"
     CDS             complement(1402..2364)
                     /locus_tag="PLP95_10410"
                     /inference="COORDINATES: protein motif:HMM:NF025587.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate and iron-dependent oxygenase
                     domain-containing protein"
                     /protein_id="HPB46254.1"
                     /translation="MSRPSHHIPVIDLSAESDEVLAQRLDAACRDRGFFVVTGHGVRS
                     ELIADLEAVARAFFALDPEVKSEIAMDDGGKAWRGWFALGSELTAGRPDHKEGIYFGS
                     ELPADDPRPLHGPNLFPSHPAEMRDVVLEYIEAVAALGRRIVALLDRGLGLDGGLVSL
                     VDDPTVLFRIFAYPPAVADGVEGWGVAEHSDYGLITLLLQDHIGGLQVASDDGWIDVE
                     PIAGSFVCNIGDMLERATGGTYRSTPHRVRNASSQLRLSFPLFFDPGWSVRVTRLIAS
                     EGAGRRRWDREDPLLFEGTYGEYLMSRVRRVFPQLGEAYLDERL"
BASE COUNT          509 a          867 c          792 g          387 t
ORIGIN      
        1 ctcatacagc gcccagtcgc tttgacactc gccgaccccg atcgcgaact cgtcggaagc
       61 gattctggcg accgcgtccc ccggccgaat caacccacgc agacgggcac cgatgcgttt
      121 caacaccagg tcacccgccg tcgagccgag gctatcgttg agcgcattga acccgtccac
      181 atcgaccacg agcacgccca cgcggccagg ctgcgccgcc gatgcgagca tctcgttgag
      241 ttcggccacg agccccgtac ggttcaacag cccggtcagt tcatcgtggc gcgagcgtcg
      301 acgcatctcg tcttcggcct ctgccgatcc gagtagttgg gcgcacgttg cggcaacggt
      361 gccgagcagg atcagatcgc tgtcggccca gtcgcccgtg cgggtgtgcc actcgaatcc
      421 catggcggcc actgcacgcc cgccgatcac gatgggcaac tccaggaacg aactgatccc
      481 caactcctcg aggaactcgc ggtcgatact ccattccgga gggagtttcg aaacatcgcg
      541 aacagcgagc acctccaacg ccgattgggc gctggcagcg agcggggtga gcgacagcgg
      601 aaccgacacg tactcgtggc gctggggcgg gatgccccaa gcggtccact catgggtcat
      661 cgtctcgaac tccgccgcgg cgtcaaagga gaccacgtac ccgcgatcgg cgccgagaaa
      721 gatcgcagcc tctccgaggc ccgactcgat gatgtcatcg acgtcgtctg caacggtgtg
      781 cgctgcgcgg tactgaaacc gggtgatgag ggtttcgagc gattcctttc tccgcaacag
      841 gcgttcggcg gaacgcgcaa ccgtcgaacg ttgcatgatc accacgatca cggaacgacc
      901 gtcgagcaca tcggcccaga tcgaaagctg aacctcgaag gtctccgaca ccgcagaaca
      961 catcgtcatc gagatctcga ccggttcgct gaggcccccg gacgatccgc tttccgacag
     1021 gcacctggcg acgcgttcgc gctccgattc gctcgccaac tcggcgaacg ggcggccgac
     1081 caattcgtcg accgagtagc cggtctcggc gctgacgacg ttggataccg acagacaggt
     1141 acccgctcga tcgaggatga acaccgggtc gtgcacgcgt tggctcagcc gctgccagaa
     1201 cgggtcaatc caatcggtct ccactgagac gtcacgcgtc atgcgactcg atcggcaccc
     1261 gtcacaggaa attgaaggat ggcacctgaa gtagcgcgaa ttggccagtc ggtggtccgt
     1321 tcggcccacc gccgcggcct ttacccaaac ggccggaccc atcgacccgg atgagtcggt
     1381 gccgcccgct cggggcacca ctcacaagcg ctcgtccagg tacgcctcac caagctgggg
     1441 aaagactcga cggacacgac tcatgaggta ctcgccgtag gtcccctcaa aaagcagcgg
     1501 atcctcgcgg tcccaacgtc ggcggccggc gccttcggaa gcgatcaggc gtgtgacccg
     1561 aaccgaccag cccgggtcga aaaacagtgg gaacgacaga cgaagttgtg aactggcgtt
     1621 tcgcactcgg tgcggggtgg accggtatgt tccgccggtc gcccgctcca acatgtctcc
     1681 gatattgcag acaaacgagc ccgcgatcgg ctccacgtcg atccatccgt catccgacgc
     1741 gacctgcagg cctccgatgt gatcctgcag caacagcgtg atcaggccat agtcgctgtg
     1801 ttcagcgacg ccccacccct ccacgccgtc ggccaccgcc ggtgggtacg caaagatccg
     1861 aaacaacacg gtcgggtcgt cgacgaggct caccagacct ccgtccaagc cgagaccccg
     1921 atcgaggagc gcgacgatgc gacgcccaag ggcggcgacc gcctcgatgt attcgagcac
     1981 gacgtcgcgc atctccgcag ggtgactcgg aaagaggttc gggccgtgaa gcggccgagg
     2041 gtcatctgcg gggagttccg aaccgaagta gatcccttcc ttgtggtcgg gacgccccgc
     2101 ggtcaattcg gaacccagcg caaaccagcc gcgccatgcc ttaccgccat cgtccatggc
     2161 gatctcgctc ttcacctcgg ggtcgagcgc gaagaacgcg cgggcaacag cctcgaggtc
     2221 ggcgatgagc tcagatcgca caccgtgtcc ggtgacgacg aaaaacccac ggtcacgaca
     2281 tgcggcgtcg aggcgctgag ccagcacctc gtcggactcg gccgacaagt cgatgaccgg
     2341 gatgtggtgc gagggccggg acaccgacga agtgttgcac gccgcgtgca gtgcgcgaaa
     2401 tggcgaggcc gtgccgcgag cgcggtcacg ccgcggcgac cacatcgagc ggggttagcg
     2461 aacggtgcgc cggagcttcg gttggaccga gaaggccgtc gacctcaagc gactgccaca
     2521 aggaccggcg aagcagcgcg gcatcggccc ccacg
//
LOCUS       DAOOMA010000411         2551 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_50151_length_2551_cov_3.871795, whole genome shotgun sequence.
ACCESSION   DAOOMA010000411 DAOOMA010000000
VERSION     DAOOMA010000411.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 2551)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2551)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2551
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50151_length_2551_cov_3.871795"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..540)
                     /locus_tag="PLP95_10415"
     CDS             complement(<1..540)
                     /locus_tag="PLP95_10415"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HPB46255.1"
                     /translation="MPDLKPTTPEALLSEVERFIADEVVPLEVQEGGNLHHASEEFRR
                     SLQAAARARGLLAPHVSREFGGHGLGLVERAPVFEAAGYSLFGPLAINIAAPDEGNMH
                     LLEMVATEEQKQVYLVPLAAGEIRSAFAMTEPAPGAGSDPSMLRTSAVPVNGGWRIDG
                     HKWFITGATGASFFICMART"
     gene            698..1846
                     /locus_tag="PLP95_10420"
     CDS             698..1846
                     /locus_tag="PLP95_10420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HPB46256.1"
                     /translation="MRRTLFTEEHDLFREAIRQFAEREIAPYYEEWELAGITPRELFT
                     KAGANGFLGMDVEEEYGGGGATDFLYNMALGEELAYAGFNGSGIGLTLHNDICVPYFR
                     TLGTEEQKQRWLPGICSGEFITAIAMTEPGIGSDLASMSTTAIRDGDSYIVNGSKTFI
                     TNGINADLIITAVKTDPTQKHKGMSLLVIERGMEGFERGRNLDKIGLHSQDTAELFFN
                     DVRVPAENLLGIEGHGFLHLVDKLAQERMNIAIYALASSEAALGWTLDYVKERTAFGK
                     PIGTFQNSRFKLAELRTEIDIAQCFVDRCIEDLSRGELDGVDAAKAKWWTTELQKRTM
                     DTCLQLHGGYGYMLEYPIARSYLDARITTIYGGTTEIMKEIIGKSLGI"
     gene            complement(1878..>2551)
                     /locus_tag="PLP95_10425"
     CDS             complement(1878..>2551)
                     /locus_tag="PLP95_10425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB46257.1"
                     /translation="SSCPTTSTGRDAVRVYEKRPSGLAVGDLRSYAATHPGEAVFAVV
                     RTFVGLAMFAFFNTRLGVVLFFVGLALLASTVPFVRRVADAAMIRPCLVLSNRPKWDG
                     VSRRVWELDAGDVDLVAVAGALSSALDGELGESIAAFEELGEFRGDVRNAVLSAEAHA
                     PGQVAWAVSGAVSIIPGRESDGVWLRLERVDHDNNASKLKVTVVSNEGPATAALATAA
                     VKVAA"
BASE COUNT          512 a          836 c          786 g          417 t
ORIGIN      
        1 cgtgcgcgcc atgcagatga agaacgatgc gccggtcgcg ccggtgatga accacttgtg
       61 cccgtcgatg cgccatccac cgttcaccgg gactgccgag gtacgcagca tcgacgggtc
      121 cgagccggca cccggcgcag gttcggtcat cgcaaacgcc gagcggattt ctccggcggc
      181 aagcggcaca agatagacct gcttctgctc ctcggtcgcg accatttcca acaggtgcat
      241 gttgccctcg tcgggggccg cgatgttgat ggccaacggc ccgaacaacg agtagccggc
      301 cgcctcgaac accggtgccc gctcgacgag tccgaggcca tgaccgccga attccctcga
      361 cacgtggggg gccagcaggc cgcgtgcgcg cgccgccgct tggagactgc gccggaactc
      421 ctccgaggcg tggtgcagat tgccaccctc ttgaacttcg agcggaacga cctcgtccgc
      481 gatgaagcgt tcgacctcgc tgagcagcgc ttccggagtg gtcggtttga gatcgggcac
      541 atggtctcct ggcgctcggt cggcgatcca gtttgccagc gtcagagatg agggagcctt
      601 cgacttcgca atttcgacag ccacggagaa gaatttgtgc cgcagtacgc gagaaccacg
      661 atcccgaagg atcgccacga cccccaggag ccacctcgtg cgccgaacac tcttcaccga
      721 agaacacgac ctgttccgcg aggcgattcg ccagttcgcc gagcgcgaga tcgcccctta
      781 ttacgaagaa tgggagcttg ccggcatcac ccctcgagag ctgttcacca aggcgggcgc
      841 gaacggcttc ttgggcatgg acgtcgaaga ggagtacggc ggcggcgggg cgaccgactt
      901 cctctacaac atggccctcg gcgaggaact ggcctacgcc gggttcaatg gttcgggaat
      961 cggcctgacg ttgcacaacg acatctgtgt gccgtatttc cgcaccctcg gcaccgagga
     1021 gcagaagcag cgctggttgc ctggcatctg ctcgggtgaa ttcatcacgg caatcgcgat
     1081 gaccgaaccg ggcatcgggt cggacctggc atcgatgtcc accaccgcca ttcgcgacgg
     1141 cgactcctac atcgtcaacg gatcgaagac cttcatcacc aacgggatca acgccgatct
     1201 catcatcact gccgtcaaga ccgacccgac ccagaaacac aagggcatgt cgctgctcgt
     1261 gatcgagcgc gggatggaag ggttcgaacg cggtcgcaac ctcgacaaga tcggtctgca
     1321 ctcgcaagac accgccgagt tgttcttcaa cgacgtgcgg gttccggcgg agaacctgct
     1381 gggcatcgaa ggccacggct tcctgcatct ggtcgacaag ctcgctcagg agcggatgaa
     1441 catcgcgatc tacgcgttgg cctcatcgga ggcagcgctc ggatggacgc tcgactacgt
     1501 caaggaacgc acggcgttcg gcaagccgat cggcaccttc cagaacagcc ggttcaagct
     1561 cgccgaactg cgcaccgaga tcgatatcgc ccagtgcttc gtcgatcgtt gcatcgagga
     1621 cctcagcagg ggcgaactcg acggggtcga cgccgccaag gccaaatggt ggaccaccga
     1681 gttgcagaag cggacgatgg atacctgcct gcagttgcac ggtggatacg gctacatgct
     1741 cgaatacccg atcgcccgct cgtacctcga tgcacggatc acgacgattt acggtggcac
     1801 gaccgagatc atgaaggaga tcatcggcaa gtcgctcggt atctaagcgc cgggtcggcg
     1861 cgggaggatt cgtctcgtta agcggcgact ttgaccgcgg ctgtggccag tgcggcggtc
     1921 gccggtcctt cgttcgacac cacggtgacc ttcagctttg aggcgttgtt gtcgtggtca
     1981 acacgttcga ggcgaagcca tacgccgtcg gactcgcgac cggggatgat cgagacggcg
     2041 ccgctgacgg cccacgcgac ctgacccggc gcgtgggcct cggccgacag cacggcgttg
     2101 cggacatcgc cgcggaactc tccgagttct tcgaacgctg cgatggactc gccgagttca
     2161 ccgtcgagcg ccgagctgag cgcacccgcc accgccacga ggtccacatc accggcatcc
     2221 aactcccaca cgcgacgcga gactccgtcc catttcggcc ggttcgacag caccagacac
     2281 ggacggatca tcgccgcgtc ggcgactcgc cgaacaaagg gcaccgtcga tgccaacaac
     2341 gccaggccga caaagaacag tacgactccc agcctcgtgt tgaagaacgc gaacatggcc
     2401 agccccacaa aggttcgaac gacggcgaat accgcctcgc ccggatgggt cgccgcgtac
     2461 gagcgaaggt cgccgaccgc caacccggat ggccgctttt cgtagacccg cacggcatcc
     2521 ctacctgtag aagtcgtcgg gcacgacgaa g
//
LOCUS       DAOOMA010000412         2548 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_50239_length_2548_cov_3.773365, whole genome shotgun sequence.
ACCESSION   DAOOMA010000412 DAOOMA010000000
VERSION     DAOOMA010000412.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 2548)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2548)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2548
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50239_length_2548_cov_3.773365"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..253
                     /locus_tag="PLP95_10430"
     CDS             <1..253
                     /locus_tag="PLP95_10430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HPB46258.1"
                     /translation="AVARAEMKVASLDHTIWFHRPMRADRWHLHDFRCHGFVRGRGLA
                     VGQIFDESGLHVASVAQEVLLRDRRERATSRSGPANSDQ"
     gene            875..2278
                     /locus_tag="PLP95_10435"
     CDS             875..2278
                     /locus_tag="PLP95_10435"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HPB46259.1"
                     /translation="MLVDVSSVILGWGLSLALPFPGAVDRRDSAVLLVAATAMLLQMG
                     AMAGMRLQKARVASRRSMELSGLGRSALAGAIWAGALARIGNLEYADERIVVGAVLSF
                     GFLAVGRGMYTSWLRTERAHGRYQRGLVLVGTNEEAASLRELLSVHPELGYRVVAVVG
                     DREGYDARDWSIPFVGPVDEALNAVAHHRADGVIIAVSALRGGELNQVSRELLRHDVH
                     VHLSSGLLGIDQRRLRPLPMAHEPLFYLEAPISAPWRRVTKRVIDVTASAVALVVISP
                     FLALAALAIKLGDRGPVLFKQERVGLNGQTFEVYKLRTMVPDAEARLDEVRQMMGNGR
                     DGVLFKLENDPRRTKVGRVLEATSFDEIPQLFNVLNGSMSLVGPRPPLPREVEMFDDE
                     LMRRFTVRPGVTGLWQVEARENPSFSAYRRLDLFYVDNWTLTIDIILMLQTCSSILAR
                     VVRRGRASVPAEDRVAA"
BASE COUNT          404 a          764 c          875 g          505 t
ORIGIN      
        1 cgcggtggcc cgcgccgaga tgaaggtcgc ctccctcgat cacaccatct ggtttcaccg
       61 tccgatgcgg gcggatcgct ggcatctcca tgattttcgg tgccacggct tcgtgcgcgg
      121 tcggggcctg gcggtcgggc agatcttcga tgagtcgggg ctgcacgtcg cgtccgttgc
      181 tcaggaagtg ctgcttcgcg accggcgcga gcgtgcaacg tctcgatcgg gccccgcgaa
      241 ttcggatcaa tagcgggttg ccgaccgcgg taaataccca agtggcagcg ttctccacat
      301 tcggtatgaa ttgtggcaca ctacaacggt gaaatttgct gtaggcggat ctctccaaac
      361 ccgtgaaagc ccacgggcac agggggagag ccattcggcc taccagaatc gacgtgctgt
      421 agtccatccg gcggttcatc gaattttccg cgcaacggtg cttgatggag tgagcagacg
      481 cgtttacgtt ggcagtcgga ggcggccact cggagccagc gtttacgccc tacgagaccg
      541 tcggccatat ttacttttat gagcgattcg gacggtttcg gatcggatcg cttcgaaggc
      601 tggggccttc accggaagct agggcgcgtg cgactggggt cgctctctag acgattgtga
      661 ttgaggaaaa gcaggcatga cgagcgagtc agtggctcca ccggaaacgg tcggtgagcg
      721 gcgaagggaa gagttcaggt cctcggacgc ctcgggcggt ccggccgtaa acgggcaggc
      781 cgacgtcctt tcgcttccgg tacgccatct tgacggtccc tttggagact cccaaggtcg
      841 cccgcaggga gcatccaccc tgaagcgttg actggtgctt gtcgatgtca gctcggtgat
      901 cctcggatgg ggcctgtcgt tggcgctgcc gtttcccggg gcggtcgatc ggcgcgattc
      961 tgcggtgttg ttggtcgctg cgacggcgat gctgctgcag atgggcgcga tggccggtat
     1021 gcgccttcag aaggctcgtg tcgcctcgag acgttcgatg gaactgtccg gacttggccg
     1081 ctctgcgctc gccggcgcga tctgggcggg tgcactcgca cggatcggca atctcgagta
     1141 cgcggatgag cgaatcgtgg tcggcgcggt gctgtcgttc gggttcctcg ccgtcggccg
     1201 aggcatgtac acgtcgtggc tgcgcaccga gcgcgcccat gggcgatacc agcgcggact
     1261 cgtgctggtc ggcacgaacg aggaagccgc ctcacttcgc gagttgttga gtgtccaccc
     1321 ggaactcggc taccgggtcg tcgctgtggt gggagatcgg gaggggtacg acgcccgcga
     1381 ctggtccatt ccctttgtcg gtccggtcga cgaggcgctc aacgcagtgg cgcaccatcg
     1441 ggccgacgga gtgatcatcg ccgtgagtgc gcttcggggc ggagaactga accaggtgtc
     1501 gcgcgaactg ctgcgccacg acgttcacgt gcacctgtcg tctggtctgc tcggaatcga
     1561 ccagcgccgc ttgcgtccgc tgccgatggc gcacgaaccg ctgttctacc ttgaggcgcc
     1621 gatttctgca ccgtggcgcc gggtcaccaa gcgggtgatc gacgtcaccg cgtcggcggt
     1681 tgccctcgtc gtgatttcac cgtttcttgc gcttgcggcg ctggccatca aactcggcga
     1741 ccgaggcccc gtgctgttca agcaggagag ggtggggctc aacggccaga ccttcgaggt
     1801 ctacaagctg cgcacgatgg tgcccgacgc cgaagcccgt ctcgacgagg tgcgtcagat
     1861 gatgggcaac ggtcgcgacg gcgtgctgtt caagctcgag aacgacccac ggcgtacgaa
     1921 ggttggccgg gtgctcgagg cgacgagctt cgacgagatc ccgcaactct tcaacgtctt
     1981 gaacggctcg atgagcctgg tggggccgcg ccccccgctg ccgcgggagg tcgagatgtt
     2041 cgacgacgaa ctcatgcgcc gcttcaccgt gcggccggga gtgaccggcc tgtggcaggt
     2101 ggaagcgcgc gagaacccgt cgttctcggc gtaccgacgc ctcgatctgt tctacgtcga
     2161 caactggacg ctcacgatcg acatcatctt gatgttgcag acctgcagct cgatcctggc
     2221 tcgtgtcgtt cgtcggggtc gggcatcggt gccagcggag gatcgcgtcg ccgcctgatc
     2281 ctggcggtcg atcctggcgg tcgggctcct cgttctggcc ccgcacctca agctgagaga
     2341 tggatttcgg gaagttcatc ctgaggtggg gttgttcgct acagtgtcgt tcgtcacact
     2401 gtcggagcgg tggggaacgg ctgtgctccc caccgaggcg cctgggtctg cgttcgatgg
     2461 acgctgaacg atcacggtgt cggccgaagc gactcggggg agatccatat gacagggcga
     2521 gccaggcaga cccacccgtc gacgccgc
//
LOCUS       DAOOMA010000413         2547 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_50262_length_2547_cov_3.813804, whole genome shotgun sequence.
ACCESSION   DAOOMA010000413 DAOOMA010000000
VERSION     DAOOMA010000413.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 2547)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2547)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2547
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50262_length_2547_cov_3.813804"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..193
                     /locus_tag="PLP95_10440"
     CDS             <1..193
                     /locus_tag="PLP95_10440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB46260.1"
                     /translation="ERSSSDTFAVAPDPALDELAELMVGATATGNVVFAVGAEETDLM
                     LRVEPMFSFDDTHTWIALG"
     gene            complement(280..810)
                     /locus_tag="PLP95_10445"
     CDS             complement(280..810)
                     /locus_tag="PLP95_10445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB46261.1"
                     /translation="MNHTARPDGAICLERAAADRLPSVVALSNRGGSDGSVRELDDDG
                     LRSDDAVPSMRPRRLWAIADAYRTVFDAWGDDEVAFNRPYLGGYETTTAGPLFRAFEP
                     RYVVRRPGHEPRRLRLGAFQNEFRREFLLGEHATAQLMQPGTDWVEPPEERVQWLAER
                     LRDAHQLVRTGRTARR"
     gene            892..1137
                     /locus_tag="PLP95_10450"
     CDS             892..1137
                     /locus_tag="PLP95_10450"
                     /inference="COORDINATES: protein motif:HMM:NF012596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HPB46262.1"
                     /translation="MGYVLLNRPAKRNARSTTLLRELAAVARWFDSVSEVRAVVISGA
                     GDHFSAGADLEAFSGPAVAKELVMTCRPFDAAEARSR"
     gene            complement(1243..1893)
                     /locus_tag="PLP95_10455"
     CDS             complement(1243..1893)
                     /locus_tag="PLP95_10455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HPB46263.1"
                     /translation="MTIRVVVADDQDLVRAGLVMILGAQPDIEVVGEAADGLSAVDLA
                     TRLRPDVLLVDIRMPGLDGVEVTRRLAGPEVADPMAVVVITTFDLDEYVLGALRAGAR
                     GFLLKDAGPDLLVQAIRAAAVGDALIAPNVTRRLLATLADRAPRTPVQPIDRLTEREE
                     EVLALVANGRTNAEIAAELFVGLSTAKTHVASLMTKIGARNRVEIAMWAYDTGRAR"
     gene            complement(1890..>2547)
                     /locus_tag="PLP95_10460"
     CDS             complement(1890..>2547)
                     /locus_tag="PLP95_10460"
                     /inference="COORDINATES: protein motif:HMM:NF019350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HPB46264.1"
                     /translation="RFRSALWQRQELEIRSEERVALARELHDIVAHHVSAIAVQAQAG
                     GAVAATQPDKAHQVFSAIESEASSALAEMRTMVRVLREGEGADFSPQPGVADLAALAR
                     PDATPAIAVSLASSVAQLPPPVDAAIYRLAQEALTNAVRHGTDATAVEIVVFSDRNTV
                     RLSVADDGRAAHGAASRPGFGLIGMEERAKLLGGTLSAGPGPEGGWLVEAVLPLDAPT
                     "
BASE COUNT          420 a          881 c          837 g          409 t
ORIGIN      
        1 cgaacggagc agctcggaca cgttcgcggt cgcgcctgat ccggcgctcg atgagctcgc
       61 cgaactgatg gtcggggcca ccgccaccgg caacgtggtc ttcgccgtcg gcgccgagga
      121 gacggacctg atgttgcggg tcgaaccgat gttttcattc gacgacaccc atacctggat
      181 agcgctcggg tagcactcca ccacggcacg aaccccatca cggcacgaac ccaatcacgg
      241 ctcgtgtcca accgtggctt ggacgagaac gcagccctgt cagcgtcgcg ccgtgcgacc
      301 cgttcgtacg agctgatgtg cgtcgcgtaa gcgttcggcg agccactgga cccgctcttc
      361 gggcggctcc acccaatctg ttccgggttg catcagctgc gccgtggcgt gttcgccgag
      421 caggaactcg cgacggaact cgttctggaa cgcgccgagc cgcagacgtc gtggttcatg
      481 gccgggacgg cgaacgacgt accgcggctc gaacgcacga aacagcggcc cggcggtcgt
      541 cgtctcataa ccccccaggt agggacggtt gaacgcgacc tcgtcgtcgc cccaggcatc
      601 gaagaccgtt cggtacgcat cggcgatcgc ccacagacgc ctcgggcgca tcgacggcac
      661 cgcgtcgtcg gatcgaaggc cgtcgtcgtc gagttcgcgc accgatccgt cggacccacc
      721 gcggttcgac aacgcgacga cgctcggcag gcggtcggcg gccgctcgct ccaggcagat
      781 cgcgccgtcc ggcctggcgg tgtgattcat cgtgtcgtga aacgacaaca gatcgagtga
      841 ggtcgccgcg ttccagtcgg caaccgtgca ggccgccggg tcgaggaccg catcggctac
      901 gtgctgttga accgaccggc caagcgcaac gcacggtcca ccacgttgct ccgggagctg
      961 gcggccgtcg cccgttggtt cgacagcgtc tcagaggttc gggccgtggt gatcagcggc
     1021 gcaggcgacc atttcagcgc cggcgccgac ctcgaagcgt tctccggtcc ggccgtggcg
     1081 aaggagttgg tcatgacctg tcggcccttc gacgcagcgg aggcccgctc gcgatagccc
     1141 gagccgcctt ttctcgttct gtaggtaatc gcggttgccc cagcaaccgc gattacctac
     1201 agaaccggga gctgtcaccc ggggaacgag gttcaattcc gatcaccggg cacgtccggt
     1261 gtcgtaggcc cacatcgcga tctccacccg gttgcgggcg ccgatcttgg tcatcaacga
     1321 tgcgacgtga gttttggccg tactcagacc gacaaaaagc tcggcggcga tctcggcgtt
     1381 ggtccgaccg ttcgcgacga gtgcgagcac ttcctcctcg cgttcggtga gccggtcgat
     1441 cggctgaacc ggtgtccgcg gcgcacgatc ggccaaggtc gccagcaacc tgcgggtcac
     1501 gttgggcgcg atcagcgcgt cgccgacggc ggccgcacgg atagcttgca cgagtaggtc
     1561 cggtccggcg tccttcagca ggaatcctcg ggcgccggcg cgcagcgcac cgaggacgta
     1621 ctcgtcgagg tcgaaggtcg tgatcacgac gaccgccatc gggtccgcaa cctctggccc
     1681 ggcgaggcgg cgtgtcacct cgacgccgtc gagcccgggc atccggatat cgacaagcag
     1741 gacatcggga cgcagccggg tggcgaggtc gacagcgctg aggccgtccg ctgcctcgcc
     1801 cacgacctcg atgtcgggtt gtgcccccag aatcatcacc aatccggccc ggaccagatc
     1861 ctgatcatcg gccaccacga cgcggatcgt catgtcggtg catccaacgg caacacggcc
     1921 tcgaccaacc agccgccttc gggccccggt cccgccgaaa gcgttccgcc gagcagcttg
     1981 gcgcgttcct ccatgccgat cagaccgaac cctggcctcg aagctgcgcc gtgagcggcc
     2041 cgcccatcgt cggcgacgct cagcctgacg gtgttgcggt cgctgaacac gacgatttcg
     2101 acggcggtcg catcggtgcc atgccgcacg gcgttggtca gtgcctcctg agcgagccgg
     2161 tagatcgctg cgtccacagg gggtgggagc tgcgctaccg agctagccag cgagaccgcg
     2221 atggccggcg tcgcgtcggg ccgcgccagc gccgccaggt cagcgactcc cggctgtggg
     2281 gagaagtcgg cgccttcacc ctcgcgcagc acccgcacca tcgtcctcat ttcggccagg
     2341 gcagaggagg cttccgactc gatcgccgag aacacctggt gagccttgtc cggctgcgtc
     2401 gcggcgaccg ctccaccggc ctgtgcctga acggcgatgg ccgacacgtg gtgcgcaacg
     2461 atgtcatgca gctcacgggc cagggccact cgctcctcgc tacgaatctc gagttcctgg
     2521 cgttgccata aggcggagcg gaaacga
//
LOCUS       DAOOMA010000414         2547 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_50274_length_2547_cov_2.949438, whole genome shotgun sequence.
ACCESSION   DAOOMA010000414 DAOOMA010000000
VERSION     DAOOMA010000414.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 2547)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2547)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2547
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50274_length_2547_cov_2.949438"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..215
                     /locus_tag="PLP95_10465"
     CDS             <1..215
                     /locus_tag="PLP95_10465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012227376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HPB46265.1"
                     /translation="GQTGKVVKPTVYIAAGISGATQHLVGMKGSKNIIAINKDPEAPI
                     FSVADLGIVGDVHKVLPAVLEAIKNR"
     gene            complement(317..1066)
                     /locus_tag="PLP95_10470"
     CDS             complement(317..1066)
                     /locus_tag="PLP95_10470"
                     /inference="COORDINATES: protein motif:HMM:TIGR03843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO1664 family protein"
                     /protein_id="HPB46266.1"
                     /translation="MSRSDDPIEGYIDTVDADSSGALEILEHGEIEVLGRMPWSSNGT
                     WLCALSDGDATCRAIYKPAAHERPLWDFPDGLWRREVATWELANDLGWNLIPPTIERD
                     GPFGVGSLQFFIHARFEEHYFTFRDDAELRPQLERLCAFDLVSNNTDRKAGHVLLTDD
                     RRIWGIDHGLNFHEDFKLRTVIWDFAGSPIPSELVEALIAFVAAPTPERLGELLSENE
                     LDAVRQRANAIIASGVFPVDPTGHRIPWPLV"
     gene            complement(1082..1579)
                     /locus_tag="PLP95_10475"
     CDS             complement(1082..1579)
                     /locus_tag="PLP95_10475"
                     /inference="COORDINATES: protein motif:HMM:TIGR03847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3090 family protein"
                     /protein_id="HPB46267.1"
                     /translation="MDSSFDFPELSIFSTGTEGPPGQRVFYLQAGTDLDVVTLRLEKQ
                     QVEALADFLEQIAVRYDMVVDEPEPMVPLESPLLTEWIVGSMLVAVDQDESRLIVIAE
                     ELLPDDADPDQPAEGAEARFVLTPAQAAGFVIGAREVVTRGRPICRLCGRPIDPDGHF
                     CPRMN"
     gene            complement(1602..2279)
                     /locus_tag="PLP95_10480"
     CDS             complement(1602..2279)
                     /locus_tag="PLP95_10480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MSMEG_4193 family putative phosphomutase"
                     /protein_id="HPB46268.1"
                     /translation="MATAKRTSARSNTRHSSPQPTTVLLVRHGQTTTTGSVLPGRSPG
                     LHLTEHGRAQAQHAAQRLATAGPIAAVYASPLERTRETAAPIAKALGLRVRTAKGLLE
                     CEFGDWTGRKLDELRKLPEWSAVQSAPSTFRFPSGESFIEMQTRICNEIASLVARHPG
                     ERIVCVSHADPIKAVVAQAQGIHLDLFQRIVISPCSITPILYGAGVPVVLSVNSTGDD
                     LSALAPS"
BASE COUNT          420 a          796 c          894 g          437 t
ORIGIN      
        1 tcggccagac cggcaaggtc gtgaagccga cggtgtacat cgccgctggc atctcgggcg
       61 ccacccagca cctcgtgggc atgaagggct cgaagaacat catcgcgatc aacaaggatc
      121 ccgaggctcc gatcttctcg gtcgccgatc tcggcatcgt gggcgacgtg cacaaggtcc
      181 ttccggccgt gctcgaagcc atcaagaatc gctgatcctc gcgatccgcc gttcaaacga
      241 cgaggccccg ctcaggcggg gcctcgtcgc gtcgtacggg cgtcgtcgcg tcgggggaga
      301 ccgagcgtcg cctggctcac acgagtggcc agggaatgcg atgtccggtc ggatcgaccg
      361 gaaacacgcc gctggcgatg atcgcgttgg cgcgttgacg tactgcgtcg agttcgttct
      421 cgctcagcaa ctcgcccagt cgttcgggcg tgggcgccgc gacgaaagcg atgagcgcct
      481 cgacgagttc gctgggaatc ggggacccgg cgaagtccca gatgaccgtt cggagcttga
      541 agtcctcgtg gaagttcagc ccatggtcga tcccccagat gcgccggtcg tcggtcaaca
      601 gaacatggcc cgccttgcgg tcggtgttgt tcgacaccag gtcgaatgcg cagagccgct
      661 cgagctgtgg ccgcagctcc gcgtcgtcgc ggaacgtgaa gtagtgctcc tcgaaacgcg
      721 cgtggatgaa gaattgcagc gagccgaccc cgaaggggcc gtcgcgttcg atcgtcggtg
      781 gaatcaggtt ccagccgagg tcgttcgcca gctcccaagt ggccacctcg cggcgccaca
      841 gcccgtcggg gaagtcccac aacgggcgct cgtgggcggc gggtttgtag atcgctcgac
      901 atgtggcatc gccgtcgctc agcgcgcaca gccaggtccc gttggaactc cacggcatcc
      961 gcccgagcac ctcgatctcg ccgtgctcca agatttcgag cgcgccagat gagtcggcat
     1021 cgacagtgtc gatataccct tcgatcgggt cgtcggatcg gctcatcgat tcgcggaacc
     1081 gtcagttcat ccggggacag aaatgcccgt cgggatcgat cggccgccca cacaaccggc
     1141 agatcgggcg accgcgggtg acgacctcgc gagcgccgat cacaaatccg gccgcttgtg
     1201 cgggggtcaa cacgaaccgg gcctcggccc cctcggcggg ttgatccggg tcggcgtcgt
     1261 cggggagcaa ctcctcggcg atgacgatca ggcgcgactc gtcctggtcg accgcgacga
     1321 gcatcgaccc gacgatccat tcggtcagga gcggcgattc gagcggaacc atcggttcgg
     1381 gctcatcgac gaccatgtcg tagcgaaccg cgatctgttc cagaaagtcg gcgagggcct
     1441 cgacctgttg cttttcgaga cgcaaggtca cgacgtcgag atcggttcct gcctgcaggt
     1501 agaagacccg ttggccgggt ggcccctcgg ttccggtgct gaagatggag agctcgggga
     1561 agtcgaagga agagtccatg atggtgccaa gcggtgcggg gtcaggacgg ggcgagggcc
     1621 gagaggtcgt cgccggtgga gttcaccgac aacacgaccg ggacgccggc gccgtagagg
     1681 atcggggtga tggagcacgg ggagatgacg atgcgctgga acaagtcgag atggatgccc
     1741 tgcgcctggg cgacgaccgc tttgatgggg tcggcatgtg agacgcacac gatccgctct
     1801 cccggatggc gagcgacgag cgatgcgatc tcgttgcaga tgcgggtttg catctcgatg
     1861 aacgactcgc cgctcgggaa tcggaaggtc gacggcgccg actgcaccgc gctccattca
     1921 gggagcttgc ggagctcgtc gagcttgcgg ccggtccagt cgccgaactc gcactcgagc
     1981 agccccttgg cggtgcggac ccgcagaccg agggccttgg cgatcggtgc cgcggtttcg
     2041 cgggtgcgtt cgagcgggga ggcatagacc gcggcgatgg gccccgccgt tgcgagacgc
     2101 tgcgccgcgt gttgcgcctg ggcgcggccg tgttcggtga ggtggagccc cggcgagcgc
     2161 cccggcagca ccgatccggt cgtcgtcgtt tgaccgtgtc ggacgagcag gacggtcgtc
     2221 ggctgagggc tcgagtgacg ggtgttcgaa cgagcagagg tgcgtttggc cgttgccatt
     2281 gccgtcacgt tagttggggt gatcgccgcc ctcggtccgc ctctggtccg cggcgttggc
     2341 ggtggagccg aactcccgac gcttcggccg cggcgcgttt cggactcggg acgcagcggc
     2401 gagtagcgtt ggcgagtcat tcaacgcacg gagaatctca gtgtcgcgtt cccatcgcac
     2461 tcgtttcaca cgtctcgcca tggtcgtcct tgcaggggcc accgtcgccg gctgctatcg
     2521 cccgccggat gccaccgaat ggggccc
//
LOCUS       DAOOMA010000415         2545 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_50322_length_2545_cov_3.829317, whole genome shotgun sequence.
ACCESSION   DAOOMA010000415 DAOOMA010000000
VERSION     DAOOMA010000415.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 2545)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2545)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2545
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50322_length_2545_cov_3.829317"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(24..680)
                     /locus_tag="PLP95_10485"
     CDS             complement(24..680)
                     /locus_tag="PLP95_10485"
                     /inference="COORDINATES: protein motif:HMM:NF013375.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HPB46269.1"
                     /translation="MIATAAPIAFVGMAGVGKSAVARRVATALGVECIDIDAEVSAVR
                     GGQSVSSIFDEIGEDEFRALETAALLDALDGARNGVRGRARRAVIATGGGVVERQRNL
                     DALRRQALVVWLRSSDDVLADRLRSSSVRRPLLDGDLEANLTRLRTRREPLYRALADI
                     EIDVGSATIPQVVEMVLSRLSAVEDPAQDGEAAQDGEAVATAVVDEVAGTGAGTGAPR
                     "
     gene            complement(744..2165)
                     /locus_tag="PLP95_10490"
     CDS             complement(744..2165)
                     /locus_tag="PLP95_10490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB46270.1"
                     /translation="MMKLVDAVTHRFPQKNPWAADALASLDDQLNPATPIHELVAGWY
                     HLGEGHQRPLLDVVFVLTDSRLGIGHPGEARVPRWIELSNITVVDAIDNSDFPLQTIE
                     LQQADGSVSLVGWPSTFSDRVIEVLLGLRDRSAPATSPLGDSIPNSLPDPMPEPFLIE
                     GATPPIAPVPAVPVESVPVESVTVQSVPVQSVPVESVPVQPAVAPAPALDFLKAPAPR
                     TAPVDLAPGVANLHGATQPEPRSEAELGADVQPGSAGDLKAGPVTSSMPVIADVDSDA
                     HDIFFGDLDAMAEAASDIPSFDADDRAPWEIMGDAWPEVVVGVNYLGGHPHIKRKRKS
                     GVMAFGPGGMEVKGTGISRWEMELDWNHVNDVEVVGADEIMFSDAIRVPADSSVVVVG
                     MIDGARLFFETVGRRPPTLRSELAPVMSMVAAGRVRRANRPRETGGQTAGSSDVAAEG
                     LAPGPTEGSVGADPSTLVGGLGQ"
     gene            complement(2199..>2545)
                     /locus_tag="PLP95_10495"
     CDS             complement(2199..>2545)
                     /locus_tag="PLP95_10495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB46271.1"
                     /translation="RMVDDVARPVAPASLEAAVADVPVLPGAPDFASMARSSDTGDIS
                     DPRLALNVSNSPIPSRGIGTLRINDDSEAELARQLAMLSPRAAEAVAGAETVTGFDDA
                     RPSRVARFFDTP"
BASE COUNT          487 a          879 c          800 g          379 t
ORIGIN      
        1 tcccaactcg acctcgacgc ggatcatcgc ggcgccccag tcccggcccc agtcccggca
       61 acctcatcca ccacggcggt ggcgacggcc tcgccgtcct gagcggcctc gccgtcctga
      121 gcgggatcct ccacggccga gagccgactc agcaccattt ccacgacctg tggaatcgtt
      181 gcggaaccga cgtcgatttc gatatcggcg agcgcacggt aaagcggctc acgccgggtt
      241 cgaaggcggg tcaggttcgc ctcgagatca ccatcgagca acggtctgcg aacagaactg
      301 ctgcgaagcc ggtctgcaag cacgtcgtcg gaggatcgaa gccacaccac gagcgcctgc
      361 cgcctcagcg catcgaggtt gcgttgccgc tcgaccaccc cgccgccggt cgcgatgacc
      421 gcccgccgag ccctgccccg tacgccgttg cgagccccgt cgagcgcatc gagcagcgcc
      481 gcggtctcga gggcacgaaa ttcgtcctcc ccgatctcgt cgaagatgga actgacgctc
      541 tgcccgcccc gaaccgcaga cacctccgcg tcgatgtcga tgcattcgac gccgagcgct
      601 gtcgccacac gccgcgccac ggcggacttg ccgacgccgg ccatgcccac gaacgcgatg
      661 ggtgctgcag tggcgatcac ggtcgacaag ggtagcggca cctcgtcaag gccggcatcc
      721 ggccgacgag cgagtggcgg ttgctactga cccaacccgc cgacgagggt cgacggatcg
      781 gcgccgaccg acccctcagt cggccccgga gccaagccct cagcggcgac gtcggaactc
      841 ccggcagttt gaccaccagt ttccctagga cggttggccc tccggacacg ccccgcggca
      901 accatcgaca tcaccggcgc gagttccgac cgaagcgtcg ggggtcgacg acccacggtc
      961 tcgaaaaaca gcctggcacc gtcgatcatg cccacgacga caacgctgga atcggcggga
     1021 actcggatgg catcgctgaa catgatctca tcagcaccga ccacctcaac gtcgttgacg
     1081 tggttccagt cgagttccat ctcccaacga ctgatcccgg tccccttgac ctccataccg
     1141 ccgggtccga acgccatgac gccgctcttg cgcttgcgtt tgatgtgcgg gtgaccaccg
     1201 aggtaattga cccccaccac gacctccggc caggcatcgc ccatgatctc ccacggagca
     1261 cgatcatcgg catcgaaact cgggatatcc gaggcggcct cggccatcgc gtccagatcg
     1321 ccgaagaaga tgtcgtgagc atccgagtcg acatcggcaa tgaccggcat cgaactggtg
     1381 acggggccag ccttgagatc gccagccgac ccgggctgga catctgctcc caactccgcc
     1441 tccgaccggg gctccggctg ggtcgctccg tgaaggttcg caacgcctgg cgcgagatca
     1501 accggagcgg tccggggggc aggggctttc aagaaatcga gcgcaggtgc cggagccact
     1561 gcaggctgaa ccggcacaga ctcgaccggc acagactgaa ccggcacaga ctgaaccgtc
     1621 acggactcta ccggcacgga ctcgaccggc accgcgggca cgggcgcgat cggcggggtc
     1681 gccccttcga tcaggaacgg ctcgggcatc gggtcgggca gcgaattcgg gatcgaatcc
     1741 cccagcgggc tcgtggccgg ggccgagcgg tcgcgcaggc cgagcagcac ctcgatcact
     1801 cggtcgctga acgtcgaggg ccaacccacg agcgacaccg aaccgtcggc ctgttgaagc
     1861 tcgatcgtct gcagggggaa atcggagttg tcgatggcgt cgacgaccgt gatgttcgaa
     1921 agctcgatcc atctcggaac ccgagcctcg cccggatggc cgatgccaag gcggctgtcg
     1981 gtgaggacga acaccacatc gagcaacggg cgctggtgcc cctcacccaa gtgataccaa
     2041 ccagccacaa gttcatgaat cggcgtcgcc gggttcagct ggtcgtccag cgatgcaagc
     2101 gcatcggcgg cccacgggtt tttctgaggg aaccgatgcg tcaccgcatc gaccagtttc
     2161 atcatcgaaa tctcactaca gagtcggagt tcggctgttc acggcgtgtc gaaaaatcta
     2221 gccacacggg atggtcgggc gtcgtcgaaa ccggtgacgg tctcagcacc agcaaccgcc
     2281 tcggcggcgc gggggctgag catggccagt tgtcgagcca gctcggcctc cgaatcgtcg
     2341 ttgatccgga gggtgccgat acctcgcgaa ggtatcggcg agttggaaac gttcagggcc
     2401 agtcggggat cggaaatgtc accagtatcg gacgagcgcg ccatcgacgc gaaatcgggg
     2461 gcgccgggaa gaaccggaac gtcggcgacg gccgcctcaa gcgacgctgg agcgacggga
     2521 cgagccacgt cgtctaccat ccgct
//
LOCUS       DAOOMA010000416         2539 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_50502_length_2539_cov_3.552335, whole genome shotgun sequence.
ACCESSION   DAOOMA010000416 DAOOMA010000000
VERSION     DAOOMA010000416.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 2539)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2539)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2539
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50502_length_2539_cov_3.552335"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..416
                     /locus_tag="PLP95_10500"
     CDS             <1..416
                     /locus_tag="PLP95_10500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB46272.1"
                     /translation="PPKVAGPDGAPADSTPEELTPYVVVASASNLTSMTIEDAAGATT
                     VVNAEYNATVRANVAVFATEYAPFDTIAGIFLSEPAAAITFTLDDARTVRVVTPAGGS
                     LSGSIPHVVVLADATTAPTRSVRAEQAGVCAAASA"
     gene            complement(481..1464)
                     /locus_tag="PLP95_10505"
     CDS             complement(481..1464)
                     /locus_tag="PLP95_10505"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HPB46273.1"
                     /translation="MAELEMMTNEPDGEAMKRVVVTGAAGFIGSHLSERLVHLCDEVI
                     GVDCFTDYYERDAKESNLEALRQVPNFRFVEADLRSDDLAPLFDGVDVVINLAASPGL
                     VRSWEEFDQYESNNLRAVQRIADAMLATGVGHLVQASTSSVYGADATGPETSETLPIS
                     PYGVTKLAGEHLLRAYGMSRGLPLTILRYFSVYGPRQRPDMAYRIFCERLIDGEEIVV
                     FGDGLQSRSNTYVSDIVEGTIAAARQAPTGEIYNIGGGREITLLEAVELLGDALGVEP
                     RIRFEEGRAGDQRRTVAQTDKARGQLGWEPSVLPAEGLALQAEWVRSLRDR"
     gene            complement(1576..2187)
                     /locus_tag="PLP95_10510"
     CDS             complement(1576..2187)
                     /locus_tag="PLP95_10510"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HPB46274.1"
                     /translation="MSNSTRTRLDPQRRRELILQEASRVFREQPYSEVSLDTVASEAG
                     VTRGLLHHYFGSKRNLYLAVLEQAVSIPAGVEIVPAGSFADLRELFDACVGLWMSTLR
                     AAGGLWPGASVGFAEADIDDVMEVARTSLVERMIDELPFPETLDRDSLSAALHAYAAF
                     ASVASDAWLRDGRLHDEQLRAMLSETLVAIAESIVPAMGAASR"
     gene            complement(2238..>2539)
                     /locus_tag="PLP95_10515"
     CDS             complement(2238..>2539)
                     /locus_tag="PLP95_10515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB46275.1"
                     /translation="INTSWSDGITGGMGWIAIALVIFGFWRPDLTLAGAYLFGGLTSL
                     NFVLQTRGVKVAPQLLSSLPYLVTIVVLVAVSAGWSRQRVGAPAALGVAYEREER"
BASE COUNT          469 a          951 c          758 g          361 t
ORIGIN      
        1 tgccaccgaa ggtcgccgga cccgacggag cgccggccga cagcactccc gaggaactga
       61 ccccctacgt cgtggtcgcc tccgcgagca acctcacgtc gatgaccatc gaggacgcgg
      121 ccggcgccac caccgtcgtc aacgccgagt acaacgcgac cgtgcgagcc aacgtcgccg
      181 tcttcgccac cgagtacgca ccgttcgaca ccatcgccgg aatcttcctc agcgagcccg
      241 cagcggcgat caccttcacc ctcgacgacg ctcgaacggt ccgggttgtg acacctgcgg
      301 gcggatcgct gtccgggtcg atcccccacg tcgtggtgct cgccgatgcc acgaccgccc
      361 cgacccgcag cgtccgcgcc gaacaggccg gcgtgtgtgc cgcggcgtcg gcctgaagct
      421 tcgcccttgc ggcgtcggcc ccgcgacgcg gcccctgcaa cgccgccccg ctgccgccga
      481 ttagcggtcg cgcagtgagc gcacccactc ggcctgcaac gccagaccct cggcgggcag
      541 caccgacggc tcccacccga gttgcccgcg ggccttgtcg gtctgagcta ccgtgcggcg
      601 ctgatcgccc gcacggcctt cctcgaaacg gatccgtggc tcgacgccga gggcgtcgcc
      661 caacaactcg acggcctcga gcaacgtgat ctcgcggcca ccgccgatgt tgtagatctc
      721 ccccgtgggg gcctgacgcg ccgcggcgat ggtgccctcg acgatgtcgc tcacgtaggt
      781 gttgctgcgg ctctgcaagc cgtcgccgaa caccacgatc tcctcgccgt cgatgaggcg
      841 ttcacaaaag atccggtagg ccatgtccgg acgttggcga ggaccgtaga ccgagaagta
      901 ccgaaggatc gtcaacggaa gtccacggct catgccatag gcccgcagca ggtgctcgcc
      961 cgcgagtttc gtcaccccgt acggagagat gggcagcgtc tcgctcgtct cggggccggt
     1021 cgcgtccgcg ccgtacaccg acgacgtcga cgcctgcacc aggtgcccga cgccggtcgc
     1081 cagcatcgca tcggcgatcc gctgcaccgc acggaggttg ttcgactcgt actgatcgaa
     1141 ctcctcccag cttcgaacca ggccgggcga ggcggccaga ttgatcacaa catcgacccc
     1201 gtcgaacaac ggcgccaggt cgtcgctacg aagatcggcc tccacgaagc ggaagttcgg
     1261 aacctgccgc agcgcctcaa ggttcgactc cttggcgtca cgctcgtagt aatcggtgaa
     1321 acagtcgacc ccgatgacct cgtcgcacag gtggacgagg cgctctgaga gatgcgaacc
     1381 gatgaacccc gcggctcccg tcaccaccac acgcttcatc gcttcaccat cgggttcatt
     1441 cgtcatcatc tccagctctg ccaccgacgc gtccatcgca cgcccgctgt gcgaggccga
     1501 aaccgtgtcg gcccagcggc ggtgggtctg tgaagcgtac ccgcgtcggg acgatccgcc
     1561 actcgccaca accgctcagc gagacgccgc acccatggcc ggcacgatcg actcggcaat
     1621 ggcgaccagc gtctcgctca acatcgcacg aagctgctca tcgtggagcc gtccgtcgcg
     1681 cagccacgcg tccgatgcga ccgaggcaaa ggccgcatag gcgtgcagcg cggcgctcag
     1741 cgaatcacga tcgagcgtct ccgggaacgg caattcgtcg atcatccgct cgacgagcga
     1801 cgtgcgcgca acctccatca cgtcgtcgat atcggcttcc gcgaacccca ccgatgcccc
     1861 cggccacagc cctcccgcgg cacgcagcgt cgacatccac aacccgacgc acgcgtcgaa
     1921 caactcgcga aggtccgcaa aggatccggc cggaacgatc tccacccccg ccggaatcga
     1981 caccgcctgc tccagcacgg cgagatacag gttgcgcttc gaaccgaagt agtgatgcag
     2041 cagcccgcgg gtgacccccg cctcgctcgc caccgtgtcg agcgacacct cggagtaggg
     2101 ctgctcgcga aacacccgcg atgcctcctg caggatcaac tcacgtcgac gctgcggatc
     2161 cagccgagtg cgagtcgagt tggacacgca gcgagcatag ggacttcacc accccggcga
     2221 cgtcacaaca cctcgcgtca gcgttcttcg cgctcatagg caacgcccaa cgccgccggc
     2281 gccccgaccc gctgacgcga ccacccggcc gacaccgcca ccagcacgac gatagtgacg
     2341 aggtacggca gcgagctgag gagctgtgga gccaccttca cgccgcgggt ctgcaacacg
     2401 aagttcaacg aggtgagtcc accgaacaga taggcaccgg cgagggtcag atccggccgc
     2461 cagaacccga agatcaccaa ggcgatcgcg atccaaccca tgccgccggt gatcccgtcg
     2521 ctccaggagg tgttgatcg
//
LOCUS       DAOOMA010000417         2537 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_50541_length_2537_cov_3.747784, whole genome shotgun sequence.
ACCESSION   DAOOMA010000417 DAOOMA010000000
VERSION     DAOOMA010000417.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 2537)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2537)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2537
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50541_length_2537_cov_3.747784"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..206)
                     /locus_tag="PLP95_10520"
     CDS             complement(<1..206)
                     /locus_tag="PLP95_10520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB46276.1"
                     /translation="MVAPQKIKPSTAGIWIGLVLLVLGPVGCISGAGYAAVKIFGELS
                     DIPSSLRSDVPSEFSVELTDVSGG"
     gene            complement(385..1527)
                     /gene="dapE"
                     /locus_tag="PLP95_10525"
     CDS             complement(385..1527)
                     /gene="dapE"
                     /locus_tag="PLP95_10525"
                     /EC_number="3.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020376944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009014 - succinyl-diaminopimelate
                     desuccinylase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinyl-diaminopimelate desuccinylase"
                     /protein_id="HPB46277.1"
                     /translation="MSVGSSPLSSSSPLSTDAVEVRDVFGLTAAMVAVASESFDEAHL
                     ADLIEAELRLLDHLDVIRVGDNVVARTNFGRPQRLVLAGHTDTVPANNNFPGHIAVDP
                     DTGDDVLHGVGSADMKGGLAVMLASARLHRDPGVDLTYVFYAREEVAAVHSGLGELFE
                     AIPEWLEGDLALLGEPTDGEVEAGCQGTMRVSVTLAGRRAHPARAWMGRNAVHRAGAL
                     LVALDAYEPRRPMLSGCQFHEALLALSIEGGVSGNVVPDAVTIMIGHRFAPDRTIAEA
                     EAHVRSVVEPFLEEGDSFEVVDAASAAAPATDHSLLATLIERNDLMVGAKLGWTDVAR
                     FAERGVPAANFGPGVALVAHTAGEYVTRTSVERTWEALDDLVNNPE"
     gene            complement(1524..>2537)
                     /locus_tag="PLP95_10530"
     CDS             complement(1524..>2537)
                     /locus_tag="PLP95_10530"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HPB46278.1"
                     /translation="LEAMASSNAERGYPASIGSPAYREAASRWVARRLGAEVGPEHLA
                     ACVGTKEFVAGLPHLLKLRRSDRDTVLYPAVSYPSYEMGAILAGCRAVAVPVDEHWRI
                     DLSAISAQDAARALCLWVNTPGNPAGGLDDLGAAAAWGRAHDVAVFSDECYVEFTWNG
                     PRRTILSHGLDGVVAVHSLSKRSNFAGARAGFYAGDPELVEYLSEIRKHQGLMVPGPV
                     QAGAIAAWDDDAHVDAQAQIYRSRLERLIGQLARLGVDASMPEGAFYLWVPAPDGDAW
                     ALAQRLAVEVGIVSSPGEFYGEQAAGFVRIAAVQPDARLDLVDARLDALGGSGLGGSE
                     LGR"
BASE COUNT          456 a          936 c          794 g          351 t
ORIGIN      
        1 aacccgccgg agacatcggt gagttcgacc gaaaactccg agggcacgtc ggaacgcagg
       61 ctcgacggga tatccgacag ttctccgaag atcttcaccg cggcgtagcc ggctccggat
      121 atgcagccga ccgggccgag aacgagcagg accaatccga tccagatgcc ggcggtcgac
      181 ggtttgattt tctgcggtgc taccactcgc tgctccttag gtcttgtggc cgaggctacc
      241 gaaccgtccc ggcaccgacg tggattaccc acgatcgcca gcacgcatcg caacggctca
      301 tcgcaacggc tcagcgatca gctgacgcgg acggtgcgat cagcggcgcg gcgacaccgg
      361 tgcaacggcc ggcggcgacc aggctcactc cgggttgttc accaagtcgt cgagggcctc
      421 ccaggtccgt tccaccgagg ttcgggtcac gtattccccc gcggtgtggg ccaccagcgc
      481 aacgcccggc ccaaagttcg ctgcgggaac gcctcgttcg gcaaagcgtg cgacgtcggt
      541 ccacccgagc ttggctccga ccatcaaatc gttgcgttcg atgagcgtag ccagcagaga
      601 atgatcggtg gccggagcgg cggccgaggc agcgtcgacc acctcaaagg aatcgccctc
      661 ctccaggaac ggctcgacga cgcttcgcac atgcgcctcg gcctcggcga tggtgcgatc
      721 cggggcgaag cggtggccga tcatgatcgt caccgcgtcg ggaacgacgt tgcccgacac
      781 acccccctcg atcgacagcg ccaacaacgc ctcgtgaaac tgacagcccg acaacatcgg
      841 ccgccgcggc tcgtaggcgt cgagggccac gagcaacgcc ccggcgcgat gcacagcgtt
      901 tcggcccatc caggcccgcg ccggatgtgc ccgcctgccg gccaacgtca ccgacacccg
      961 catcgtgccc tggcatcccg cctcgacctc gccgtcggtg ggttcgccga gcagggcgag
     1021 gtcgccttcg agccactcgg gaatcgcctc gaacagttcg ccgaggccgc tgtgcaccgc
     1081 ggcgacttct tcgcgtgcgt agaacacata ggtcaggtcc acgccgggat ctcgatgcag
     1141 ccgagccgag gccaacatca ccgcgaggcc gcccttcatg tcggcggaac cgaccccgtg
     1201 cagcacgtca tcgccggtat ccgggtcgac ggcgatgtgg ccgggaaagt tgttgttcgc
     1261 cggcaccgtg tcggtgtggc cggcgagcac caaccgctgg ggccgcccga aattcgtgcg
     1321 ggccacgacg ttgtcgccga cccggatgac gtcgaggtga tcgagcagcc gcaactcggc
     1381 ctcgatcaga tcggcgagat gcgcctcgtc gaaactctcc gacgcgaccg ccaccatcgc
     1441 cgcggtcaat ccgaacacgt cgcgaacctc gacggcgtcg gtcgacaacg gcgaggacga
     1501 agacaacggc gaggaaccga cgctcatcgg cccagctccg aaccgcccag ccccgaaccg
     1561 cccagcgcat cgagtcgggc gtcgacgaga tcgagtcgag cgtccggctg aaccgcggcg
     1621 atgcgcacga accctgccgc ctgctcgccg tagaactccc ccggactcga cacgatgccg
     1681 acctcgaccg ccaggcgctg ggccagcgcc caggcgtcgc cgtcgggggc cggcacccag
     1741 aggtagaacg ccccctccgg catcgaagca tcgacgccga ggcgggccag ttggccgatc
     1801 agtcgctcga ggcgcgaccg gtagatctgc gcctgagcat cgacatgtgc gtcgtcatcc
     1861 cacgccgcga tcgcacccgc ctgcaccggc ccgggcacca tcaggccctg gtgtttgcgg
     1921 atctccgaca ggtactccac cagctcgggg tcgcccgcat agaacccggc acgcgccccc
     1981 gcgaagttcg accgcttcga cagcgaatgc accgcgacca ccccgtcgag gccgtggctc
     2041 agaatcgtgc gcctcggccc gttccaggtg aactccacat aacactcgtc ggaaaacacc
     2101 gctacgtcgt gggctcgccc ccacgccgcg gccgcgccca gatcgtcgag cccgcccgcc
     2161 ggattgcccg gcgtgttcac ccacagacac agcgctcgcg ccgcgtcctg cgcgctaatc
     2221 gccgacaggt cgattcgcca gtgctcatcg accggcacgg ccacggcacg acaccccgcc
     2281 agaatcgcgc ccatctcata ggacggatac gaaacggccg ggtacaacac cgtgtcgcgg
     2341 tcggatcggc gaagcttcaa caggtgtggc aaccccgcca cgaactcctt ggtgcccacg
     2401 cacgcagcga ggtgttcggg cccgacctcg gcgccgagtc ggcgcgccac ccaacgagac
     2461 gccgcctcgc ggtaggccgg cgagccgatc gacgccggat agccacgctc ggcgttcgac
     2521 gatgccatcg cctcgag
//
LOCUS       DAOOMA010000418         2537 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_50549_length_2537_cov_2.967768, whole genome shotgun sequence.
ACCESSION   DAOOMA010000418 DAOOMA010000000
VERSION     DAOOMA010000418.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 2537)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2537)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2537
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50549_length_2537_cov_2.967768"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..343
                     /locus_tag="PLP95_10535"
     CDS             <1..343
                     /locus_tag="PLP95_10535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HPB46279.1"
                     /translation="DADVTPTFTRNGTPLSYGQTRRLAPDILRRVLSYRDRGCRFPGC
                     ERDTVGCDVHHIVQFNDEGPTDPANLTHLCRFHHSLHHRGGAVISGAPNERHRAVRPD
                     GTTITNTPHYL"
     gene            complement(393..1208)
                     /locus_tag="PLP95_10540"
     CDS             complement(393..1208)
                     /locus_tag="PLP95_10540"
                     /inference="COORDINATES: protein motif:HMM:NF014671.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HPB46280.1"
                     /translation="MARRRFVRTMALRHHDGASTRQPVQVVVEEPMEIRLDGHPVSTT
                     MRTPGHDFELAVGFCHGEGLLGGAAVQSVRYCGTGSAVQTGFNVVSVATGGWAPEPAT
                     ESATEQNSNGCGLSGSGAVESLSERLDPLVATPFDIALLERIEQRAERERTLLAETDG
                     SDSAAVFGVRDGRVLVVREDIDLGNAVDKVVGRLLLDGRLPALIAGGEPLGLWVSGRL
                     GFELARRAWAGGFVVLAAGGAASSLALEVAERANLTLVSFARGATATVYPAAR"
     gene            complement(1295..>2537)
                     /gene="groL"
                     /locus_tag="PLP95_10545"
     CDS             complement(1295..>2537)
                     /gene="groL"
                     /locus_tag="PLP95_10545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443099.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HPB46281.1"
                     /translation="VSIEVETEEQFAQVASISSADSEIGALIAEAITKVGKDGVITVE
                     ESNTFGMGLDFVEGMRFDKGYIAPYMVTDAERMEAVLESPYILFVSSKISAIRDLVPV
                     LEKVMQTGKPLLIIAEDVEGEALATLVVNKIRGTFNSVAVKAPGFGDRRKAMLADMAI
                     LTGGQVISEEVGLKLDTADLALLGQAERVVITKDETTIISGAGDSDEIAGRVAQIKGE
                     IDNTDSDYDREKLQERLAKLSGGVAVLKVGAATEVELKEKKHRIEDAVSTTKAAIEEG
                     VVAGGGVTLLRAQEAVNKVVESLEGDEATGARIIAKALEGPLTQIAENAGLDGGVVVN
                     AVRNLSNPAEGLNAATGEYVNLVDAGIIDAAKVTRSGLQNAASIAALFLTTMAVITDI
                     PEPAALSMPGGGMGDMGGMGF"
BASE COUNT          423 a          907 c          785 g          422 t
ORIGIN      
        1 cgacgctgat gtcacgccca ccttcacccg aaacggcaca ccactttcct acgggcagac
       61 gcgtcgcctc gcccccgaca tcttgcggcg agttctttca tatcgcgacc gcgggtgcag
      121 gttccccgga tgcgaacgcg acacggttgg ctgcgacgtg caccacatcg tccaattcaa
      181 cgatgagggc cccaccgacc ccgccaatct cacccatctc tgccggttcc accactcgct
      241 tcaccaccgc ggcggggcgg tcatatcggg cgccccgaac gaacgacacc gagccgttcg
      301 ccccgacgga acgaccatca ccaacacccc gcactaccta tgacctcaca gcgccatgag
      361 ctcacagcgc catgagctca cagcgcccgc aactaccggg ccgctgggta gaccgtcgcc
      421 gtcgcacccc tcgcaaagct gaccaacgtg aggttggctc gttcggccac ctccaacgcc
      481 aatgagctcg cggcgccccc tgcggccagg accacgaaac cgccggccca cgcccgtcgt
      541 gccaactcga aacccaaacg ccccgaaacc cacagtccga gcggctctcc cccggcgatg
      601 agcgcaggaa gcctgccgtc gagcagcagc cgaccgacga ccttgtcgac ggcgttgccg
      661 aggtcgatgt cttcgcgcac caccaaaacc cggccgtcgc gaacaccaaa caccgccgcc
      721 gagtcgcttc catccgtctc ggccagcagc gtgcgctccc gctcggcccg ctgttcaatg
      781 cgttcgagca gcgcgatgtc gaacggagtc gccaccagcg ggtcgagccg ctcgctcagc
      841 gactcgaccg cccccgaacc cgagaggcca caaccgttgg agttctgctc ggtcgccgac
      901 tcggtcgccg gttccggggc ccacccgccg gtggccacgc tgacgacgtt gaaccccgtc
      961 tgcaccgccg agccagttcc gcagtagcga accgactgca ccgccgcccc gccgaggagg
     1021 ccttcgccgt gacagaaccc gaccgccaat tcgaagtcgt ggccgggggt gcgcatcgtc
     1081 gtggagacgg ggtgcccgtc gagacggatc tccatcggct cctcgacgac cacctgaacg
     1141 ggctggcgcg tcgaggcccc gtcgtggtgt cgcagcgcca tcgtgcgcac gaaacgtcgt
     1201 cgagccatat tcccagcgta cgtcggtcgc ataacgcgac gggtccccca cggcacacgc
     1261 cgtgagggac ccgtgagcga ggatcgctgg tggcttagaa gcccatgccg cccatgtcgc
     1321 ccatgccacc gcctggcatg ctgagcgctg ctggctcggg gatgtcggtg atgaccgcca
     1381 tcgtcgtgag gaacagcgcc gcgatcgagg cggcgttctg caggccggaa cgggtcacct
     1441 tcgcagcatc gatgatgccg gcatccacca ggttgacgta ctccccggtg gccgcgttga
     1501 ggccctcggc cgggttcgac aggttgcgca ccgcgttgac cacgacgccg ccgtcgaggc
     1561 cggcgttctc cgcgatctgg gtgagcgggc cttcgagcgc cttggcgatg atgcgtgcac
     1621 cggtcgcctc gtcgccttcg agggactcga cgaccttgtt cacggcttcc tgggcacgaa
     1681 gcagggtgac gccgccgccg gccaccacgc cctcttcgat cgcagccttg gtggtggaca
     1741 ccgcgtcttc gatgcggtgc ttcttctcct tgagttccac ctcggtcgcc gcgccgacct
     1801 tgagcaccgc gacaccgccg ctgagcttgg cgaggcgctc ctggagcttc tcgcggtcat
     1861 agtcggaatc ggtgttgtcg atctcaccct tgatctgagc gacacgaccg gcgatctcgt
     1921 cggagtcgcc ggcgccggag atgatggtgg tctcgtcctt ggtgatgacg actcgttcgg
     1981 cctggccgag cagggcgaga tcggcggtgt cgagcttgag gcccacctct tcgctgatga
     2041 cctggccgcc ggtgaggatc gccatgtcgg cgagcatggc cttacggcgg tcgccgaagc
     2101 ccggcgcctt gacggcaacc gaattgaagg tgccgcggat cttgttgacc acgagcgtgg
     2161 cgagggcctc gccttcgacg tcctcggcga tgatgagcaa cggcttgccg gtctgcatca
     2221 ccttttcgag gacgggaacc aggtcgcgga tcgccgagat cttcgacgac acgaacaaga
     2281 tgtagggcga ctcgagcacg gcttccatgc gctctgcgtc ggtgaccatg tagggagcga
     2341 tgtagccctt gtcgaagcgc atgccttcga cgaaatcgag gcccataccg aaggtgttgg
     2401 attcctcgac ggtgatcacg ccgtccttgc cgaccttggt gatggcttcg gcgatgagcg
     2461 cgccgatctc gctgtcggcc gacgagatgg aggcgacctg ggcgaactgc tcctcggtct
     2521 caacctcgat ggacacc
//
LOCUS       DAOOMA010000419         2536 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_50566_length_2536_cov_3.437727, whole genome shotgun sequence.
ACCESSION   DAOOMA010000419 DAOOMA010000000
VERSION     DAOOMA010000419.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 2536)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2536)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2536
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50566_length_2536_cov_3.437727"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..642)
                     /locus_tag="PLP95_10550"
     CDS             complement(<1..642)
                     /locus_tag="PLP95_10550"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HPB46282.1"
                     /translation="MTSIEAGAGDGAAAGATGAGGGARGGARRRTSLDALRGIAVALV
                     IAYHVDPVVVPGGFIGVDLFFVLSGYLITTLLLVEHGRSQRIDLRSFYVRRIRRLWPL
                     GWTVVALICLAGLGNVWSPDQQRGLPSSALAAIAQVANWHQINSGGYVTAFVGPSPFQ
                     HYWSLAIEEQFFVVWPLVVMWLLRRRAVGWFPAGLMVMIVASVLANEAAGSFAR"
     gene            complement(685..1266)
                     /locus_tag="PLP95_10555"
     CDS             complement(685..1266)
                     /locus_tag="PLP95_10555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB46283.1"
                     /translation="MRTRFGGRPTSTVAVIATFTVVSLFGSAAVGCTRTRDVDLSDTT
                     STTIRRISDDRLADAGSVASAEAVGQVQALVNRLLGVDDPCAILNENAIQDYTLDGSS
                     LASSAVRRALSSGVTAVYNHTINLIPQDDSTMIAALRVQRETLIDVLDVVDRYATNPT
                     SNEAANQVRTLVTGEEFVKSAEVISLWLGSNCL"
     gene            complement(1301..1918)
                     /locus_tag="PLP95_10560"
     CDS             complement(1301..1918)
                     /locus_tag="PLP95_10560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB46284.1"
                     /translation="MTSAQRNMVIVVGVVICVTLGCARSTADGVRRIELVAGDTSAPT
                     SSVGLAAEVSGEGSGSSEDSVAGEESVANSSEAASSPSVRAANASRGGSAADEQFAAT
                     LDRALESGDLCVVWDLLMSVRLSAENDDVLAEAMRLVVRVMGLSSRIVPSALAEDWDL
                     VTDRIEEMAESVSSGDKDAASLAYHDTGFVHAQEEIAQWTSVKCG"
     gene            complement(2093..>2536)
                     /locus_tag="PLP95_10565"
     CDS             complement(2093..>2536)
                     /locus_tag="PLP95_10565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB46285.1"
                     /translation="VAAAGHEPPSRVITATEALDAVQRARDSGDLCELVGALMMPLPT
                     SAGGDAEASDEEGELYQNLAAMVGSSADLVPTDDADFPTLVRDWEAVGDALNVAATEL
                     TRLPGDLTNPVLVAALRSDQATSALRNLERFDRVRCGEAGPNTGA"
BASE COUNT          480 a          904 c          767 g          385 t
ORIGIN      
        1 cctcgcgaac gacccggccg cctcgttcgc gaggacggat gccacgatca tcaccataag
       61 cccagcggga aaccaaccga ccgcacggcg cctcaacaac cacatcacca cgagtggcca
      121 gaccacgaaa aactgttcct cgatcgccag gctccagtaa tgctgaaacg gcgagggacc
      181 gacgaaggcc gtgacgtacc caccggagtt gatctggtgc cagttggcga cctgcgcaat
      241 cgccgccagc gccgaggacg gcaacccccg ttgctggtcc ggagaccaca catttcccag
      301 cccggccaga cagatgagcg ccacgactgt ccagccgagg ggccacagcc gacggattcg
      361 ccgcacgtag aacgatctca ggtcgatgcg ctgtgatcgc ccgtgttcga ccaacaacag
      421 cgtggtgatc aaatagccgg agaggacgaa aaacaggtcg acgccgatga atccgccggg
      481 aaccacgacc gggtcgacgt ggtacgcgat gacgagcgcg acggcaatac cccgcagtgc
      541 atcaagcgat gtccgccggc gagcaccgcc acgggcgcca cccccggcac cggtagcacc
      601 agcagcagcg ccatctccgg caccagcctc gatcgaggtc acgaccatcg ttcgactccc
      661 cgaacgccta cgatgcgcgg cggctcagag gcagttgctt cccagccaca gactgatgac
      721 ctcggcggat ttcacgaatt cctccccggt caccaacgtc cgcacctggt tcgccgcctc
      781 gttcgacgtc gggttggtcg catagcgatc gacgacgtcg aggacatcga tcagcgtctc
      841 gcgctgcact cggagcgcag cgatcatcgt cgaatcgtcc tgcgggatca ggttgatcgt
      901 gtggttgtac accgccgtca cgccactcga cagagcgcgg cgcaccgcgg aactggcgag
      961 cgaactgccg tcgagggtgt agtcctgaat cgcgttctcg ttcaggatcg cgcagggatc
     1021 gtcgacgccg agcaggcgat tcaccaacgc ctgaacctga ccgaccgcct cggcactcgc
     1081 caccgacccg gcgtcggcca accgatcgtc ggaaattcgc cggatcgtcg tactcgtcgt
     1141 gtcggaaaga tcgacgtcgc gggtccgcgt gcacccgacc gccgccgaac cgaacaacga
     1201 caccaccgtg aacgtcgcaa tcacggcgac ggtcgaggtg ggtcgacctc cgaatcgggt
     1261 cctcaccact cagcatcctc cgtcaaccgc gtgtcgggtc tcatccgcat ttgaccgagg
     1321 tccactgcgc tatttcctct tgagcgtgaa cgaacccggt gtcgtgatac gccagcgacg
     1381 ccgcatcctt gtcacccgag gacaccgact cggccatttc ctcgatccgg tcggtgacca
     1441 gatcccagtc ctcggcgagc gccgacggaa cgattcggct gctcagcccc atcacccgga
     1501 cgaccaggcg catcgcttcg gcgaggacgt cgtcgttctc ggcgctcaaa cgaaccgaca
     1561 tcagcaggtc ccacacgaca cacaggtcgc cggattccaa cgctcggtcg agagtcgcgg
     1621 cgaactgttc atcggctgcg gaaccgccac gactcgcgtt cgcggcgcgc accgacggcg
     1681 agctcgccgc ctcggaagag ttcgccaccg attcctcgcc cgcaaccgag tcctcgctcg
     1741 atcccgaacc ttcgcccgac acctccgcgg caaggcccac cgacgatgtc ggggccgagg
     1801 tgtcgccggc gacgagttcg attctgcgca ccccatcggc cgtgctgcgt gcgcaaccca
     1861 aggtcacaca gatcacgacc ccgacgacga tcaccatatt gcgttgagcg gatgtcatcg
     1921 gggtgctccg taggcgagcc gggactcggt gtgtttcgcg tccctgggct cacgagtgca
     1981 agcaggcttg cgatcagtca gtccggcggt accgaccctg cagcatacgg aagatccccg
     2041 aacactcaag gcccaatgta tgaacgctct caccgaaacc acacgctgag actcatgcgc
     2101 cagtgttcgg gcctgcctcg ccgcaacgca cccggtcgaa ccgttcgagg ttgcgcagcg
     2161 ccgaggtcgc ctggtcggag cgcagcgcgg cgacgagcac cggattggtc aggtcgcccg
     2221 ggagcctcgt cagttcggtt gccgcgacgt tcaacgcgtc accgaccgcc tcccagtcgc
     2281 ggaccagcgt cggaaaatcg gcgtcgtcgg tcggcacgag gtcggcggag gatccgacca
     2341 tcgcggcgag gttctggtag agctcgcctt cctcgtcgct cgcctcggca tcgccgccgg
     2401 ccgaggttgg aaggggcatc atcagcgccc cgacgagctc gcacaggtcg ccggagtcgc
     2461 gcgcccgctg caccgcgtcg agcgcctcgg tcgcggtgat cacccgcgag ggcggctcgt
     2521 gcccagcggc cgcgac
//
LOCUS       DAOOMA010000420         2533 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_50649_length_2533_cov_3.586360, whole genome shotgun sequence.
ACCESSION   DAOOMA010000420 DAOOMA010000000
VERSION     DAOOMA010000420.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 2533)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2533)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2533
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50649_length_2533_cov_3.586360"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..536)
                     /locus_tag="PLP95_10570"
     CDS             complement(<1..536)
                     /locus_tag="PLP95_10570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HPB46286.1"
                     /translation="MTDLTAAAQALSAAQNVVDSGIARLAEIGIDDNQVLAYDVAHGA
                     AAVQTGQSLLNSYGDKGEIEAKITVAFIADAIAEIAGKVFGREEEWGVGSDAFDTARS
                     FVSTYRKPEFLASLAGEQGPRHLSDDFEMVQDTFRRFADEKLKPIAEHIHRSNGDIPE
                     EIIEGLAEMGAFGLSIPE"
     gene            761..1930
                     /locus_tag="PLP95_10575"
     CDS             761..1930
                     /locus_tag="PLP95_10575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB46287.1"
                     /translation="MAGKWRLARRFALATVALVLGALVAPAEYAGAQDDAGSGALQRS
                     LFVSGSPALPGSGVSVWADGCPANSSVFLSLFTADQGNLLALNVVTSSVAGAWSAVLT
                     VPDDVRPGTYLLTARCVGAGGYFGYNGYNGYNGYNGYNGYNGYNGYNGYNGYNGAGFY
                     YAPAEVVVRPGPNPPASDGDLEVTPEVIAPGASARVRGEGWAPGEQVTIVLYSDPVVL
                     LSTAANGSGKVDAEVTVPAATPVGDHRMWAINATSTSHRPRALSAAVRIARSDSSTSS
                     TSSTTTTTTPAGATSTTSPGSTEATDSPTTTTSSLLPNTSPTSTSQPTAVAVRSESSQ
                     RGTGGSSGTGGGASPIAFTGASSVELLLIALGVMCLGASVMVGVRRRVASPSLHD"
     gene            1941..>2533
                     /locus_tag="PLP95_10580"
     CDS             1941..>2533
                     /locus_tag="PLP95_10580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB46288.1"
                     /translation="MTVGWARRSDGTWTECGFAASPQRLSILLWDSAGGGAQHIDLRR
                     VVALDAFAEPVDGLLEIELRLETGETLSAWWPTAFVDEVLGHLVTLFPPPTIDGAGRY
                     QTADRDRSVAVADGRPVASAAPRFRRWVAIASAATLAVAGLNTGVLWHRVDREPMVTA
                     LASDSADTWLFIGSDDRGAVPVGANVAAFGDSNAVPGR"
BASE COUNT          391 a          799 c          875 g          468 t
ORIGIN      
        1 tcctcgggga tcgacagtcc gaaggcgccc atctccgcga gaccttcgat gatctcctcg
       61 gggatgtcgc cgttggagcg gtggatgtgc tcggcgatcg gcttgagctt ctcatccgcg
      121 aagcgccgga aggtgtcctg caccatttcg aagtcgtcgg agaggtggcg ggggccctgc
      181 tcaccggcga gggaggccag gaactcgggc ttgcgatacg tcgacacgaa tgaacgggcg
      241 gtatcgaacg cgtcggatcc gacgccccat tcctcttccc gcccgaacac cttgccggcg
      301 atctcggcga tcgcgtcggc gatgaacgcc acggtgatct tcgcttcgat ctcgcccttg
      361 tcgccgtagg agttcaacag gctctggccc gtctgcaccg ccgcggcgcc atgcgcgacg
      421 tcataggcca gcacctggtt gtcgtcgatg ccgatctcgg cgagacgggc aatgcccgag
      481 tcgaccacgt tctgtgcggc cgaaagtgcc tgtgctgcag cggtgaggtc agtcatggct
      541 cagatgttac cgcccggtaa ccgactccga caggttgcgt gccggtgctg gtgccgggtg
      601 atgcgccgtc gaccggcgcg tgcggaggcc gttcgtcaaa cgggtgccga ggttttcctt
      661 gacgcgggaa tgtggatgat tgctatattc ggcgacgtcg atttgacggg atcttcgggc
      721 ctcgtttgga gcgacattca attgaggggc agggattaat atggctggaa agtggcgttt
      781 ggcgcgccgg tttgcgctcg cgaccgtcgc gttggtgctc ggggctcttg tcgccccggc
      841 ggagtatgcg ggggcgcagg acgacgcggg atcgggagct ctgcagcgct cattgttcgt
      901 ttccggcagc ccggctttgc ccggatccgg ggtgagtgta tgggcagacg ggtgtccggc
      961 gaattcgagc gtattcctca gcctgtttac cgccgatcag ggcaaccttc tcgcgctcaa
     1021 tgtcgtcacc tcgtccgtgg ccggtgcgtg gtcggcggtc ctgacagtcc cggacgatgt
     1081 ccggccgggg acctatctgt tgacggcgcg gtgcgtggga gctggcggct atttcggcta
     1141 caacggctac aacggctaca acggctacaa cgggtacaac ggctacaacg ggtacaacgg
     1201 gtacaacggg tacaacggct acaacggagc agggttctac tacgcaccgg ccgaggtcgt
     1261 ggtgcggccg gggcccaacc cgccagcgtc tgacggcgac cttgaagtca cgcctgaggt
     1321 gatcgccccc ggcgcaagcg cacgagttcg cggcgaggga tgggcccccg gggagcaagt
     1381 gacgatcgtg ttgtactcag atccagtggt gctgctttcg acggccgcaa acgggtccgg
     1441 caaggtcgac gccgaggtca cggtgccggc tgcgaccccc gtcggggatc accgcatgtg
     1501 ggcgatcaac gcgacatcca cgtcgcatcg gccgcgcgcg ctgagcgcag cggtacgaat
     1561 cgctcggtcc gattcatcga cctcatcgac ctcgtcgacc acgaccacta cgacgcccgc
     1621 cggtgccacc tcgaccacgt ccccggggtc gaccgaagca accgatagcc cgaccaccac
     1681 cacgtcgagc ctcctgccca acacctcgcc cacgtcgacc tcgcaaccga ccgcggtcgc
     1741 ggttcgctcg gagtcgtccc agcgaggcac cggcggatcc agcggtacgg gaggcggtgc
     1801 atcgccgatt gcgttcaccg gtgcatcgtc ggtcgaactc ctgttgatcg cgctcggcgt
     1861 gatgtgcctg ggcgcctcgg tgatggttgg ggtccgccgt cgtgtcgctt caccgtcgct
     1921 acacgactga tcaatctccg atgaccgtcg ggtgggctcg caggtccgat ggaacctgga
     1981 ccgagtgtgg gttcgcagcg tcgccgcaac ggctctcgat tctcctctgg gacagcgcag
     2041 gaggaggggc acaacacatc gatcttcgcc gggtcgtggc gctcgatgcc ttcgccgaac
     2101 cggtggacgg acttctcgag atcgagttgc gacttgagac gggcgaaacc ctctcagcgt
     2161 ggtggccgac cgccttcgtc gacgaggtgc tcggtcacct cgtcacgctg ttcccgccac
     2221 caacgatcga cggtgcgggc cgttaccaga ccgcagatcg cgaccggtcg gtggcggtcg
     2281 ctgacggcag gccggtggca tcggcggccc cgcggtttcg acgatgggtc gcgatcgcga
     2341 gtgctgccac gctggcggtc gcgggcctga acaccggggt gttgtggcac cgggtggacc
     2401 gcgaaccgat ggtgaccgcg ttggcgagcg actcggcgga cacctggttg ttcatcggtt
     2461 ccgacgatcg tggcgcggtg ccggtcggtg cgaacgttgc agcgttcggc gattccaacg
     2521 cggtgcccgg gcg
//
LOCUS       DAOOMA010000421         2532 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_50676_length_2532_cov_3.349616, whole genome shotgun sequence.
ACCESSION   DAOOMA010000421 DAOOMA010000000
VERSION     DAOOMA010000421.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 2532)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2532)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2532
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50676_length_2532_cov_3.349616"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..719)
                     /locus_tag="PLP95_10585"
     CDS             complement(<1..719)
                     /locus_tag="PLP95_10585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB46289.1"
                     /translation="MAKKRSGGIPLLLTTVLSIAACVAVIIAGWKPVLGLDLQGGVSV
                     VLQPVADGEKLTDISADTLEQTKRIIQTRVDAIGVAEPEISVQGSTIVLELPGSKDQE
                     QVLELVGRTAELRFRPLVAASTNAPPADAEERVAKLRAQFNVPEGVTAESIYNESYGI
                     TGTDATGTDATGTDGLVAEDGGQITTDDGTVITEGSESTPTTAAVSGDGTGGGRKAPL
                     QTTTAAPTTAAPTTAADPAADP"
     gene            complement(789..1118)
                     /gene="yajC"
                     /locus_tag="PLP95_10590"
     CDS             complement(789..1118)
                     /gene="yajC"
                     /locus_tag="PLP95_10590"
                     /inference="COORDINATES: protein motif:HMM:TIGR00739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HPB46290.1"
                     /translation="MTTNLATLLAQNSSGGGLGALLPLIIFIPLLYMLIVPQRKQRKQ
                     HAELMDSLAVGDEVVTSGGIYGTISYIESDEPIVHLSVDTDAVIRVTRASIARKVSAD
                     AAEPDKS"
     gene            complement(1174..2289)
                     /gene="tgt"
                     /locus_tag="PLP95_10595"
     CDS             complement(1174..2289)
                     /gene="tgt"
                     /locus_tag="PLP95_10595"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020379062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - queuine tRNA-ribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HPB46291.1"
                     /translation="MKLHIDIEATDGAARSGTVRTRRGSFKTPVFMPVGTRGTVRAVT
                     TRDMDDLGAEIILGNTYHLMLRPGADVIDHFGGLHGFETWGGHMLTDSGGFQIFSLNP
                     KVDADGATFQSTYDGSYHRVTPEDAVRIQELLGADIQMVLDVCSALPASRDSLRAALE
                     TTADWAARARRSHGTFDKPDIVERQALFGIVQGGTEVDLRVESAQRTVELDFDGYAIG
                     GLSVGESREVMLPALDAALAHLPVDQPRYFMGLGDPVGIIEAVARGVDMMDCVLPTRL
                     ARHGTILTGAGKLNIKRAEFIRSDDPLDPECGCQVCERYSRGYLRHLIAVNEPSAATL
                     CTLHNLAWLLGFMDDIRAAIGAGTLSDLRAEVAAVWQ"
     gene            complement(2306..>2532)
                     /locus_tag="PLP95_10600"
     CDS             complement(2306..>2532)
                     /locus_tag="PLP95_10600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="S-adenosylmethionine:tRNA
                     ribosyltransferase-isomerase"
                     /protein_id="HPB46292.1"
                     /translation="LFISEGFDWKVVDAMLTNFHLPRSTLLVMIDAFTGSHWRDLYAH
                     ALANGYRFLSFGDAMLLGRRSDASERLVRR"
BASE COUNT          404 a          825 c          855 g          448 t
ORIGIN      
        1 gggtccgcgg cagggtctgc tgcggtcgtc ggggccgcgg tcgtcggggc cgcggtcgtc
       61 gtctgcaacg gggccttgcg gccaccgccg gttccgtcgc cggagaccgc ggccgtggtc
      121 ggcgtcgact cgctgccctc ggtgatcacg gtgccgtcgt cggtggtgat ctgcccacca
      181 tcctcggcga cgaggccgtc ggtgccggtc gcatcggtgc ccgtcgcgtc ggttccggtg
      241 atcccgtagg actcgttgta gatcgactcc gccgtcacgc cctcgggaac gttgaactgc
      301 gcacggagct tggccacacg ctcctcggcg tcggccggag gcgcattggt cgaggcggcg
      361 acgagcgggc ggaaccgcaa ctcggccgta cggccgacga gttcgagcac ctgctcctga
      421 tccttgctgc cgggcaactc caacacgatc gtggaaccct ggacgctgat ctcgggctcg
      481 gccacaccga tggcgtcgac gcgggtctgg atgatccgct tcgtctgttc gagggtgtcg
      541 gccgaaatgt cggtgagctt ctcgccgtcg gccaccggct gcagcaccac cgacactcca
      601 ccttggaggt cgaggccgag cacgggcttc caaccggcga tgatcaccgc aacgcacgct
      661 gcgatcgaca gcaccgtggt cagcagcagc gggatgccgc cggaacgctt cttcgccatg
      721 tattgccttc gctgttcgtc tgagtgtttc gggggtggtg acgtcgggtc cgtgtggccc
      781 cgttggcgtc aggacttgtc gggctccgca gcgtccgcgg acaccttacg ggcgatggat
      841 gctcgggtaa ctcgaatgac cgcgtcggtg tcgaccgaga gatgaacgat cggctcgtcc
      901 gattcgatgt agctgatcgt gccgtaaatg cccccgctgg tcacgacctc gtcgcccacg
      961 gccaggctgt ccatcaactc tgcgtgctgc ttgcgctgct ttcgctgcgg cacgatcagc
     1021 atgtacagca gcgggatgaa gatgatgagc ggaagcagtg ccccaaggcc gccgccggag
     1081 gagttctgtg cgagcagggt tgcgagattg gtggtcacga agggtgcagc ctagtgccac
     1141 gtgtgcgttc cgtggtggtc ctggccccgg gcgctactgc cagaccgccg cgacctcggc
     1201 gcgcagatcc gacagtgtgc cggccccgat cgcggcgcga atgtcgtcca tgaagccgag
     1261 cagccaggcg aggttgtgca acgtgcacag ggtcgcggca ctcggttcgt tgaccgcgat
     1321 gaggtgacga agatatccac ggctgtagcg ctcacacacc tggcagccgc actcggggtc
     1381 gagcgggtcg tcgctgcgga tgaactcggc gcgcttgatg ttgagtttgc ccgctccggt
     1441 gagaatcgtg ccgtggcgtg ccagacgggt gggcaacaca cagtccatca tgtcgacccc
     1501 ccgtgccacc gcttcgatga tgcccacggg atcgccgagc cccatgaaat agcgcggttg
     1561 atcgacggga aggtgtgcga gcgccgcgtc gagcgccggc aacatgacct cgcgggactc
     1621 accgaccgac agcccgccga tcgcataacc gtcgaagtcc aactcgaccg tgcgttgggc
     1681 cgactcgacg cgcaggtcga cctcggtgcc tccctgcacg attccgaaca aggcttgacg
     1741 ctcgacgatg tcgggtttgt cgaaggtgcc gtgactgcgc cgcgcccgcg ccgcccagtc
     1801 ggcggtggtc tccagcgccg ctctcagcga gtcgcgcgag gcgggaagcg cactgcacac
     1861 atcgagcacc atctgaatgt cggcgccgag cagctcctgg atgcgcaccg cgtcctcagg
     1921 ggtgacacgg tggtagctgc cgtcgtaggt cgactggaac gtggccccgt ccgcatcgac
     1981 cttcgggttg agcgagaaga tctgaaatcc gccggagtcg gtgagcatgt gcccacccca
     2041 cgtctcgaac ccgtgcaaac caccgaaatg gtcgatcacg tccgctcccg gacgcagcat
     2101 caagtggtag gtgttgccga ggatgatctc cgctccgagg tcgtccatgt cgcgggttgt
     2161 caccgcccgc acggtgccac gggttccgac cggcatgaac accggcgtct tgaagcttcc
     2221 ccgacgggtc cgcaccgttc cgcttcgggc ggccccgtcg gtggcctcga tatcgatgtg
     2281 caacttcacg tgatgcccct ttggctcaac ggcggaccaa ccgctcggat gcgtccgacc
     2341 ggcgccccaa gagcatggcg tccccgaacg acaagaagcg gtatccgttc gccagtgcgt
     2401 gagcgtagag gtctcgccag tgcgacccgg tgaaggcgtc gatcatcacg agcagggtcg
     2461 agcggggcag gtggaagttg gtcagcatcg cgtcgacgac cttccagtcg aacccctcgg
     2521 aaatgaacag gt
//
LOCUS       DAOOMA010000422         2516 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_51139_length_2516_cov_3.283218, whole genome shotgun sequence.
ACCESSION   DAOOMA010000422 DAOOMA010000000
VERSION     DAOOMA010000422.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 2516)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2516)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2516
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51139_length_2516_cov_3.283218"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..933
                     /locus_tag="PLP95_10605"
     CDS             <1..933
                     /locus_tag="PLP95_10605"
                     /inference="COORDINATES: protein motif:HMM:NF013003.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HPB46293.1"
                     /translation="SPVTVWTEAGRAGYNGTTVDNGHPLCSSQVGASFGEWFYVHQTE
                     ADLQSSGRRSVVIDSATSFHGATFDYSNQTLPSIVTLRGMKWSDADFSDATFINVGFG
                     CQQATNARFSGATFTSTAVGVDASHWYVVDFKNSDFSGTTWTGVVMSDVDFSGSDFSN
                     ATISNWSLENQLDFDDDGQPDNDYPSPYHLLRLDNTNWNGARIGMDVNPGTGNDAVIL
                     SGTFNNTPFKSVSLAGADLSNSEIGYVVMSGANLTGITTNNTVIHGPGFFGGSDFSGG
                     WVGATWTSPSVYYDFSAATCPDTSIYDPNTPCLG"
     gene            complement(903..1580)
                     /locus_tag="PLP95_10610"
     CDS             complement(903..1580)
                     /locus_tag="PLP95_10610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB46294.1"
                     /translation="MSTSNRLGPAGETLMTPLAAELLERCGYNPERVLTDTGNVTGAI
                     CRSIAIDDVTIAFAAAHPDAQIVTAGIGLCTRHDRRETFRVGGRARRRQLPPEDRKEG
                     ICRSGDLAEVDIGDLPKADDAVERRLRHRRGLWGETHPPVGLHRGEHGVRIGCGDAGP
                     DQTAQERVLNGWAGDCCNVAKGQEPVNRGDVMVVCVLDEVRPRRWIEVVSPERPASHP
                     RHGVFGS"
     gene            1694..2224
                     /locus_tag="PLP95_10615"
     CDS             1694..2224
                     /locus_tag="PLP95_10615"
                     /inference="COORDINATES: protein motif:HMM:NF012787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HPB46295.1"
                     /translation="MDTERFELDASSWVNSEPINLEDLLGTVVLIEAFQMLCPGCVSH
                     GIPQAAKVHRTFKRSDVVVIGLHTVFEHHDVMGPDALRAFVSEYRIPFPVAVDRPVAG
                     RSIPSTMGRYQLQGTPTALILDRSGRLRHMFLGVIDDLALGSHLGHLIAEPAEPAPAL
                     PTHPATDNPPTMEEQP"
     gene            2221..>2516
                     /locus_tag="PLP95_10620"
     CDS             2221..>2516
                     /locus_tag="PLP95_10620"
                     /inference="COORDINATES: protein motif:HMM:NF022995.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protoglobin domain-containing protein"
                     /protein_id="HPB46296.1"
                     /translation="MTTDIAAYTYGNPDLDRSPVSLDDLQALQQSLLWSDSDREALRR
                     AGAILVPQTEDILDVWYGFVGGTSHLVATFAGADGEPDGDYLGRVRARFGQWIA"
BASE COUNT          468 a          898 c          717 g          433 t
ORIGIN      
        1 tccccggtca cggtctggac ggaagccggc cgcgccggct acaacggcac caccgtcgac
       61 aacggacatc ccttgtgcag ttcgcaggtc ggagcgagct tcggcgaatg gttctacgtc
      121 caccagaccg aggccgacct ccagtcgagc gggcgacgct ccgtcgtgat cgactcggca
      181 acctcgttcc atggtgcgac gttcgactac tcgaaccaga cgctgccctc gatcgtcacg
      241 ctccgcggaa tgaagtggtc cgacgccgac ttctccgacg caaccttcat caacgtcgga
      301 ttcggctgcc agcaggcgac gaacgcccga ttctccggag cgacgttcac ctcgacggcg
      361 gtcggcgtcg atgcatcgca ctggtatgtc gtcgatttca agaacagcga cttctccggc
      421 accacgtgga ccggcgtcgt catgtccgac gtcgatttct ccggatcgga cttctcgaac
      481 gcgacgatca gcaactggtc actcgagaac caactcgact tcgacgacga cggccaaccc
      541 gacaatgact acccgtcgcc gtaccacctg ctgcggctcg acaacacgaa ctggaacggc
      601 gcccggatcg gcatggacgt caacccgggg accggcaacg atgccgtcat cttgtccggc
      661 acgttcaaca acaccccgtt caagtcggta tccctcgcgg gcgccgacct gtcgaactcc
      721 gagatcggct acgtggtcat gagcggggcc aacctgaccg gcatcaccac gaacaacacc
      781 gttatccacg gacccggctt cttcggcggt tccgacttct ccggcggttg ggtcggcgcc
      841 acctggacga gcccgtcggt gtattacgac ttcagcgccg cgacctgccc ggacaccagc
      901 atctacgacc cgaacacccc atgcctgggg tgagacgccg ggcgctcggg tgacacaacc
      961 tcgatccagc ggcggggccg cacctcatca agtacgcaca ccaccatcac atcgccgcgg
     1021 ttgaccggtt cttgaccctt ggcgacattg cagcaatcac ctgcccaacc gttgagaacg
     1081 cgttcctgcg ctgtctgatc aggaccggcg tctccacaac cgatgcgtac gccgtgctcg
     1141 ccgcggtgaa ggccgaccgg cgggtgagtt tcgccccaga ggccgcgtcg atgtcggagg
     1201 cgtcgctcta cggcgtcatc ggctttcggc aggtcaccga tgtctacctc tgccagatcg
     1261 ccggagcggc agatgccctc cttgcgatct tcgggtggaa gttgtcgccg acgagcgcgt
     1321 cccccgacgc gaaatgtttc gcgacggtcg tggcgggtac acaatccgat gccggccgtc
     1381 acgatctgcg cgtctggatg cgccgccgca aaggcgatgg tgacgtcgtc gatcgcaatg
     1441 gaccggcaga tcgcgcctgt gacgttgcct gtgtcggtca acacccgctc cgggttgtaa
     1501 ccgcagcgtt cgagcagctc agccgcgagc ggcgtcatca acgtctcgcc cgccggtcca
     1561 agtcgattcg acgtactcat gcgcgcaccg tgcgccgccc tgaaggacaa atggaaggcc
     1621 tgccgaccaa tggccttggg gcactccacc agattcctgt tgacatcatc cataggagtc
     1681 ctatactcct ggaatggaca ctgaacgatt cgaactcgac gcctcctcct gggtgaacag
     1741 tgagcccatc aacctcgaag accttctcgg aacggtggtc ctgatcgagg ccttccagat
     1801 gctctgcccc ggatgcgtca gccatggaat cccccaggcc gcaaaggtcc accgcacgtt
     1861 caaacgcagc gatgtcgtcg tgatcggact ccacaccgtg ttcgaacacc acgacgtgat
     1921 gggccccgat gcgctgcgtg cgttcgtctc ggaataccgc attccctttc ccgtggccgt
     1981 cgaccgaccg gtggctggcc gctcgatccc gtcgacgatg ggccgctacc aactccaagg
     2041 cacgccaacc gcgctgatcc tcgaccggtc gggacggctg cgacacatgt tcctcggagt
     2101 gatcgacgac ctcgcgctcg ggagccatct cggtcacctc atcgccgagc ccgcagagcc
     2161 agcacccgcg ctcccgaccc accccgcgac agacaacccg cccaccatgg aggaacaacc
     2221 atgaccaccg acatcgccgc gtacacctac ggcaaccccg acctcgaccg ctccccggtc
     2281 tccctcgacg atctccaagc actccaacaa tcactgctct ggagcgattc agaccgcgaa
     2341 gcgcttcgtc gggccggtgc catcctcgtc ccgcagaccg aggacatcct cgacgtctgg
     2401 tatggatttg tcggcggaac cagccacctc gtcgccacct tcgccggagc cgacggggaa
     2461 cccgatggcg actacctcgg cagggtgcgg gctcggttcg gccaatggat cgccga
//
LOCUS       DAOOMA010000423         2514 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_51176_length_2514_cov_4.497763, whole genome shotgun sequence.
ACCESSION   DAOOMA010000423 DAOOMA010000000
VERSION     DAOOMA010000423.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 2514)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2514)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2514
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51176_length_2514_cov_4.497763"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..280)
                     /locus_tag="PLP95_10625"
     CDS             complement(<1..280)
                     /locus_tag="PLP95_10625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HPB46297.1"
                     /translation="MNDVPQHNLAAVAEAVAQRVPDRTAIVAKGHRFTYDELNRRTRQ
                     LAHVLHDHGLGARPGGREGLASHESHQDHLAIYAYNGNEYVESMHAAFR"
     gene            525..>2514
                     /locus_tag="PLP95_10630"
     CDS             525..>2514
                     /locus_tag="PLP95_10630"
                     /inference="COORDINATES: protein motif:HMM:NF021445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerol lysyltransferase
                     domain-containing protein"
                     /protein_id="HPB46298.1"
                     /translation="MGRIVVLAGLWCIIAVPLSLPSSGVAPALVDWVNRGFGLLNIPT
                     DANLFTGAMLGSIGSALVVRKRAALWFVAVGFQGGWLLAALAVTVLRTAEVGAVPKVL
                     LDTPVEVVLFAIETVVAVVLLWVFWRVRGAFTARVAPRALRRSVLALLVGIAISAGIG
                     IVLSELAPGSLHDRFVRAEWAIRTAFGLGADPDIELIRGTGDPWVATAIGAMSTAALV
                     YAGWVFLRSSRLTDFVDTDDEIAVRTLLADYGENDSLGYFATRRDKAVVFSSDRRAAV
                     TYCVEAGASLASADPIGDEASWPSAIEQWRRVAGHHGWEPAVLSASSRGAEAYRRAGL
                     RVLSIGDEAVIEVSAFDIDAAALRPVKRAAARIERAGYHIDRRTHADLDADDLRSLAD
                     AAERWRGDEPERGFSMALGRLGDPADGRCVVVRATDATGDLRAFLSFVPWGRRGLSLD
                     LMRRDPDADNGVIEAMVCSLVDTARHVGIDRISLNFAMFREVFSDSERIGAGPVVRMT
                     ASLLTAASRWWQLETLYRSSAKYQPRWEPRFLCFSEPLQLNRALLASGIAEGFIALPG
                     RSSRAMPQPASSPALVAAAAELAQRRPRRVDRRLPPQAESRLSKAAQLSAEGSTPYAT
                     SVPWRDQVAEVRQRFAQLSDGEVSSVEVTVAGRITALRK"
BASE COUNT          358 a          848 c          854 g          454 t
ORIGIN      
        1 cccggaacgc cgcgtgcatc gactcgacgt attcgttgcc gttgtaggcg tagatggcga
       61 ggtggtcctg gtgggactca tgagaggcga gaccctcacg accacccggt cgagcaccga
      121 gcccatgatc gtgcaggacg tgtgccaatt gacgggtgcg ccgattgagc tcgtcgtagg
      181 tgaatcggtg ccccttcgcc acgatggccg ttcgatccgg cacccgttgc gccaccgctt
      241 cggctaccgc cgcgaggttg tgttgtggca cgtcgttcat cgatccccct gtgatcacaa
      301 atcggtcggc ccggcggccg caaggtcgac gatgcgaccc gaatggtgtc gacatgatgg
      361 cacttgtcgc ggcttggatg tcgaacgcga gcagtacgat tcgacctgcg atgccaagcg
      421 ctcgaacacc ccacacgacg agtcgacccg ggcgagcccg agcatcctcg acggcgaccg
      481 gcgccccggc ggttccgtca tggggccgac cgatcgtcac ctggatgggt cgcatcgtgg
      541 tcctcgccgg gctctggtgc atcatcgccg tgccgctctc gctgccgtcc tccggcgtcg
      601 ctcccgcgtt ggtcgactgg gtgaaccggg gcttcggcct gctcaacatc ccgaccgacg
      661 ccaacttgtt caccggggcg atgctcgggt cgatcggcag cgcgctggtc gttcgcaaac
      721 gcgccgcgct gtggttcgtc gccgtcggat tccagggtgg ctggttgctc gccgccctgg
      781 cggtcaccgt gttgagaacc gcggaggtcg gcgccgttcc caaagtcctg ctcgacaccc
      841 ctgtcgaagt cgtgctgttt gcgatcgaga ccgtcgtggc cgtcgtgttg ctgtgggtgt
      901 tctggagggt acggggagcg ttcacggcgc gcgtcgcccc acgggcattg cgacgatccg
      961 tgctcgccct cctcgtcggc atcgccatct ccgccggcat cggcatcgtg ttgtcggaac
     1021 tggcacccgg gtcgctgcac gaccggttcg ttcgcgccga atgggcgatt cgcaccgcgt
     1081 tcggcctcgg cgccgacccc gacatcgaat tgatccgcgg caccggcgac ccgtgggttg
     1141 ccaccgccat cggggccatg tcgacagcag cactggtgta cgcgggctgg gtgtttctgc
     1201 gctcgtcgcg gctcaccgac ttcgtcgaca ccgacgacga gatcgccgtg cggacgctgc
     1261 tggccgacta cggggagaac gattcgctcg ggtacttcgc gacacgtcgc gacaaggccg
     1321 tggtgttctc ctccgaccgt cgcgccgcgg tgacctattg cgtcgaagcc ggcgcgagtc
     1381 tggcgtccgc cgacccgatc ggcgacgagg cgtcctggcc ctcggccatc gaacaatggc
     1441 gacgggtcgc cgggcaccac ggctgggaac cggcggtgct ctccgcatcg agtcgcggcg
     1501 ccgaggccta tcgtcgcgcc gggctccggg tgctctcgat cggcgacgaa gcagtcatcg
     1561 aggtgtcggc cttcgacatt gacgccgcgg cgttgcgccc ggtgaagcgc gccgccgcac
     1621 gaatcgagcg cgccgggtac cacatcgacc gacgcaccca tgccgacctc gatgccgacg
     1681 acctacggag cctcgccgat gccgccgaac gctggcgcgg tgacgaaccc gagcgcgggt
     1741 tttcgatggc cctcggccgt ttgggcgacc cggcggatgg tcgatgcgtg gtcgtgcggg
     1801 cgaccgacgc gaccggtgac ttgcgggcgt ttctctcgtt cgtgccgtgg gggcgacgcg
     1861 ggttgtccct cgacctcatg cgtcgcgacc ccgacgccga caacggtgtg atcgaggcga
     1921 tggtgtgctc cctcgtcgac accgctcgcc atgtcggaat cgaccgaatc tcgctgaact
     1981 tcgccatgtt tcgcgaggtg ttcagcgaca gcgaacgcat cggcgccggt cccgtcgtgc
     2041 ggatgaccgc atcactgctc actgcggcat cgaggtggtg gcagttggaa acgttgtatc
     2101 gatccagcgc caagtaccag cctcgctggg agccacgctt cctgtgtttc agcgagccgc
     2161 tgcagctcaa tcgtgccttg ttggcctcgg gaatcgccga agggttcatc gctctgccgg
     2221 gtcgatcgtc ccgggcgatg ccgcagcccg cgtcaagtcc ggcgctcgtg gcggccgccg
     2281 cagaattggc gcaacgacgt ccgcgccggg tcgaccgtcg tctgcctccg caggccgaga
     2341 gtcgactgtc caaggcggca caactcagtg ctgaagggtc gaccccctat gccacctcgg
     2401 tgccctggcg ggaccaggtc gccgaggtgc ggcaacgatt cgcccagctg agcgatggcg
     2461 aggtgtcgag cgtggaggtg accgttgccg gacggatcac cgcactgcgc aaga
//
LOCUS       DAOOMA010000424         2511 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_51281_length_2511_cov_2.991450, whole genome shotgun sequence.
ACCESSION   DAOOMA010000424 DAOOMA010000000
VERSION     DAOOMA010000424.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 2511)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2511)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2511
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51281_length_2511_cov_2.991450"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(47..1222)
                     /gene="carA"
                     /locus_tag="PLP95_10635"
     CDS             complement(47..1222)
                     /gene="carA"
                     /locus_tag="PLP95_10635"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005951 - carbamoyl-phosphate synthase
                     complex [Evidence IEA];
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HPB46299.1"
                     /translation="MATTATAPSQPATTPATSAVSSPGGAGHPAAELRLGHLVLADGS
                     VFEGELFGAEPEGGIATGELVFNTVMSGYQEVISDPSYAGQVIAFTTAHLGNYGTNDD
                     DNEASVVHCRGVVLREMARRESSWRSSKSLESYLVDHAIPAIGGVDTRRLTRIIREQG
                     AMGCAFGTAPVDELAAAAKQARLTDGCDLVNVVTTSEPYEVEGDPSRHVVAIDCGIKS
                     TIVDCLTRISRVTVVPADTTAEEILALDPDGVFLSNGPGDPAAVENLTPTINALVDEV
                     PMFGICMGHQLIGQALGGRTHKLAFGHHGGNHPIKDLETGRVEITSQNHNYCVDLDGV
                     DNVVITHVNLNDQTLAGFRCTDRPVFGIQYHPEAGPGPHDSHYLFERFADLIDANRS"
     gene            complement(1341..2285)
                     /locus_tag="PLP95_10640"
     CDS             complement(1341..2285)
                     /locus_tag="PLP95_10640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB46300.1"
                     /translation="MYQILSGVPTEPVDGHVDVSFERREGWVEEMGATVLPILFGDEH
                     HGPFVVMSYIAPTDEQMPLSFAHAHASDNWRISVRGTTNMGRDAYEQGQFRSHDGGVP
                     YASDNFAWGPDGGYGIIMFADRRGFAIRPVKAEIAEVVTPQQEFNGAMLGIDMQDPCP
                     GAPAIATTLGSTVRAHLDGGFDSADSWEQVTPGVRMAVGLAGEPTSGPVILLLDCDAG
                     AEALGQRSTGTESVVAPVSGSVEIGAATLAQGDVRVEEADAQHERLIAGPEGAQLVVI
                     VGDRRALGAALEAATWSGAVGQALAPILDRLQQGLVLR"
BASE COUNT          404 a          859 c          820 g          428 t
ORIGIN      
        1 acgactggtt cgcgtcacga ctggttcgcg tcacgaccgg ttcgcgtcag gaccggttgg
       61 cgtcgatgag gtcggcgaag cgctcgaaca ggtagtggct gtcgtgaggg cccggtccgg
      121 cttcggggtg gtactggatt ccgaacaccg ggcggtcggt gcagcggaac cctgcgaggg
      181 tctgatcgtt gagattgacg tgggtgatca ccacgttgtc gaccccgtcg aggtccacgc
      241 agtagttgtg gttctgactg gtgatctcca cccggccggt ttcgaggtcc ttgatcggat
      301 gatttccgcc gtggtgcccg aaggccagct tgtgggtccg cccgccgagg gcctggccga
      361 tgagttggtg gcccatgcag atgccgaaca tcggcacctc gtcgacgagc gcgttgatcg
      421 tcggcgtgag gttctccacc gccgcggggt cacccgggcc gttcgacagg aacaccccgt
      481 cgggatcgag cgccaggatc tcctcggcgg tggtgtctgc cggcacgacg gtcacgcggg
      541 agatgcgggt gaggcagtcg acgatcgtcg acttgatccc gcaatcgatc gccacgacgt
      601 gtcgactcgg atcgccctcc acctcgtagg gctcggaggt ggtcaccacg ttgacgagat
      661 cgcagccgtc ggtgaggcgt gcctgtttcg ctgcggcggc caattcatcg accggagcgg
      721 tgccgaacgc acagcccatc gccccctgtt cgcgaatgat gcgggtcagg cggcgggtgt
      781 cgactccgcc gatggcggga atcgcgtggt cgacgagata gctctccagg ctcttcgacg
      841 agcgccagct cgactcacgc cgcgccatct cgcgaagcac cacgccgcgg caatgcacga
      901 ccgacgcctc gttatcgtca tcgttggtgc cgtaattgcc gaggtgggcc gtggtgaagg
      961 cgatgacctg gccggcatag gaggggtcgc tgatgacctc ctgatagccg ctcatgacgg
     1021 tgttgaagac caactcgccc gtcgcgatgc cgccctcggg ttcggctccg aacaattcgc
     1081 cctcaaagac cgaaccgtcg gcgagaacca ggtgtcccag gcggagttcg gctgccggat
     1141 gcccggcgcc tcccgggctg ctcaccgcag acgtcgcggg ggtggtggcc ggctggctcg
     1201 gggccgtggc ggtggttgcc atctgtactc ctctgtctgt ccgtacgagt gtcccgcact
     1261 cgtttcacgg tcaggcagcc accctatcag cccaccccac ccgttctccg ataacgagtg
     1321 gtcgtgatca cgaccactcg ttatcggaga acgaggcctt gttgcaggcg atcgaggatc
     1381 ggagcgagcg cttggcccac cgcccccgac caggtcgccg cctcgagcgc cgcacccaac
     1441 gcgcgccggt cgcccacgat cacgaccaac tgggcgccct cgggaccggc gatcaatcgc
     1501 tcgtgttgag catcggcctc ctcgacacga acgtcgccct gagcgagggt cgctgcgccg
     1561 atctccaccg aacccgacac cggggcgacg accgattcgg tgcccgtcga ccgctggccc
     1621 aacgcctcgg cgccggcgtc gcagtccaac agcagaatca ccggcccact cgtcggttca
     1681 cccgccaggc cgaccgccat gcgcaccccc ggggtcacct gctcccagga atcggcggaa
     1741 tcgaaaccgc cgtcgaggtg ggcacgaacc gtggacccca gcgtggtggc gatcgcgggc
     1801 gcaccggggc acgggtcctg catgtcgatg cccaacatcg caccgttgaa ctcctgttgc
     1861 ggagtcacga cctcggcgat ctcggccttc accggacgga tggcgaaccc gcgccggtcc
     1921 gcgaacatga tgatcccgta gccgccgtcg gggccccagg cgaagttgtc gctcgcatac
     1981 ggcacgccgc cgtcgtggga gcggaactgc ccctgttcat acgcgtcgcg ccccatgttg
     2041 gtggtgccac gcaccgagat ccgccagttg tcggacgcat gagcgtgagc gaatgacagc
     2101 ggcatctgct cgtcggtcgg agcgatgtag ctcatcacga cgaacgggcc gtggtgttcg
     2161 tcgccgaaca ggatcggcag caccgtggcc cccatctcct cgacccaacc ctcacggcgt
     2221 tcgaacgaca catcgacatg gccgtcgacc ggctcggtcg gaactccgga cagaatctga
     2281 tacatcgaag gcacccccct gatctgcgcc tgccccggat cgtaggtcgc cggcggcacc
     2341 gctgcgccgg aatcgcttca aacaccgaca ctcgattggg tgatcctgcc cgaggcaggc
     2401 ctgggtcgga cgtcccatct catcgggcaa ccctcccgtt gccctcaacg cggctgcgcc
     2461 cagaccgacg aagtccgggt accaacacgc tgccggtgcc ttctggtgcg g
//
LOCUS       DAOOMA010000425         2502 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_51535_length_2502_cov_4.090315, whole genome shotgun sequence.
ACCESSION   DAOOMA010000425 DAOOMA010000000
VERSION     DAOOMA010000425.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 2502)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2502)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2502
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51535_length_2502_cov_4.090315"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(394..2022)
                     /locus_tag="PLP95_10645"
     CDS             complement(394..2022)
                     /locus_tag="PLP95_10645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FadD3 family acyl-CoA ligase"
                     /protein_id="HPB46301.1"
                     /translation="MRAGAGDAPGSIAELLARTVEQLGDQPAVVDGDTRLTYSELDER
                     SRRFGAALVEGGVAVGDRVAIWAPNSGEWIVAVLGLWRIGAVLVPINTRFKGREAAEI
                     LRRSGATALVTVTDFLDTDLLDLVRDSGVELPDLRTTVVISGPVPDGAVGWDEFVAGE
                     SESALAESARTESALAEVDRRGAALSADDRSDILFTSGTTGVPKGVVQTHGRTLCVAL
                     DWVEMTGLRGGDRYLMVNPYFHMFGLKAGILAAIAAGATMYPEAVFDVDRVLDRVESD
                     AITVLPGAPTIYQSILDHSSVGSRDLSSLRVAVTGAADIPVELIRRMHTELPFEVIVT
                     GYGLTEGGTAVATSPGDDFEVIATTVGRPRPSFELRIVDSDGAELPACEAGEIVLRGA
                     SIMLEYLDDPGATADTLTADGWLSTGDLGALGEDGNLRIMGRSKDMFIVGGFNAYPAE
                     IENSLLRHPAAQQVAVIGIPDERLGEVGMAFVVRAPGVEATEAELIAWSRAEMANYKA
                     PRRSAFVDELPINATGKVVKEQLRQQAAASAPSD"
     gene            complement(2019..>2502)
                     /locus_tag="PLP95_10650"
     CDS             complement(2019..>2502)
                     /locus_tag="PLP95_10650"
                     /inference="COORDINATES: protein motif:HMM:NF016777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HPB46302.1"
                     /translation="NDWMGSDTEFLPFQPQAFRMLAQWRPIEDSVSALIVHGALSRFP
                     TLKVAVVENGSSWVEPLLKNLADIYKKMPQDFLENPIDVFKRNIYVSPFWEEDLGALA
                     DLIGVDHVLFGSDYPHPEGLADPASYVNELEGLPDETVRKIMGANLARLMNVEDAVLR
                     "
BASE COUNT          456 a          876 c          763 g          407 t
ORIGIN      
        1 catccgcacg acggtcgggc gggatacgcc gagcacggcg gcagcctgct cggtcgtaag
       61 gctgtcgaaa gaacgcagca ccaaggggcg ttcgccgtcg gcggcggccg ctagaacttc
      121 agccagcgcc tcggcaacgg cgtcgggaag atcgagcacc gtgccgtcct cagccgtgac
      181 ccgagggcgg ctcccggcgc ggtcaagcgc ctgccgtacc cgatcacgct cttcggtagt
      241 tagcgcgctc acggcaacca ctctacccct aaacgaaaca aacgaacact cctccgtttc
      301 ttcctgccgg ctccttcaaa ctttctgcca aaagcgacac cacgtgtcac ttacggcaga
      361 aagttcgcgt gaacgcgctc gtgagtcagt gggtcagtcg ctcggggcgg acgcggcggc
      421 ttgttggcgg agctgttctt tcacgacctt gccggtcgcg ttgatcggta gctcgtcgac
      481 gaaggcggat ctccgcggcg ccttgtagtt cgccatctcg gcgcggctcc acgcgatgag
      541 ttccgcctcg gtcgcctcga ctccgggagc gcgtacgaca aatgccatgc ccacctcacc
      601 cagtcgctcg tcgggaattc cgatgaccgc gacctgctgc gccgcagggt gacgcaagag
      661 cgagttctcg atctcggcgg gataggcgtt gaacccgccg acgatgaaca tgtccttcga
      721 acggcccatg atccgcagat tgccgtcctc gcccagcgca ccgaggtcgc cggtgctcag
      781 ccaaccgtcg gcggtcagcg tgtcggcggt ggcgcccgga tcgtcgaggt actccaacat
      841 gatcgaggca cctcgcaaga cgatctcccc cgcctcacag gccggtagct cggcgccatc
      901 ggagtcgacg atgcgcaact cgaaactcgg gcgcggccga ccgaccgtcg tggcgatgac
      961 ctcgaagtcg tcccccggcg aggtggccac cgcagtacca ccctcggtga ggccgtaccc
     1021 cgtgacgatg acctcgaacg gcaactcggt gtgcatgcgc cgaatcaact cgaccgggat
     1081 gtctgccgct ccggtcacgg cgacacgcag actcgacagg tcacgcgatc cgaccgacga
     1141 atgatcgagg atcgactgat agatcgtcgg cgcccccggc aacacggtga ttgcatcgga
     1201 ctccacccga tcgagcaccc gatcgacatc gaagaccgcc tcgggataca tcgtcgcccc
     1261 cgcggcgatc gccgcgagaa tgcccgcctt caacccgaac atgtggaagt acgggttcac
     1321 catgaggtag cgatccccac cacgaagccc cgtcatctcc acccagtcga gcgccacaca
     1381 cagggtgcga ccgtgcgtct gcacaacccc cttcggaacg cccgtcgtgc cggaggtgaa
     1441 caggatgtcg ctgcgatcgt ccgcgcttaa cgcggcgccg cgtcgatcga cctccgccag
     1501 cgcggattcg gtccgtgctg attctgcgag cgctgattcg ctctcgccgg ccacaaactc
     1561 gtcccagccg accgcaccat cgggaaccgg accggagatg accaccgtcg ttcgtagatc
     1621 gggcagctcc accccgctgt cgcgcaccag gtcgaggaga tcggtgtcga gaaagtcggt
     1681 gacggtcacg agtgccgtgg cgccgcttcg ccgcaggatc tccgccgcct cgcgtccctt
     1741 gaagcgcgtg ttgatcggca cgagcaccgc gccgatgcgc cacagcccca acacggcaac
     1801 gatccactcg ccggagttgg gtgcccaaat ggccacgcgg tcgcccaccg ctaccccacc
     1861 ctcgacgagc gccgcgccga accttcgcga gcgctcgtcc agttcggaat aggtgagccg
     1921 ggtgtcgccg tcgacgaccg ccggctggtc accgagttgc tcgaccgtgc gggccaacag
     1981 ctcggcgatc gaacccggcg cgtcgcccgc tcccgccctc atcgcagcac cgcgtcctcg
     2041 acgttcatga ggcgggcgag gttggcaccc atgatcttgc gcacggtctc atcgggaagc
     2101 ccttcaagct cgttgacgta gctggccggg tcggccaacc cctcgggatg cggatagtcg
     2161 gaaccgaaca acacgtgatc gacgccgatc aaatcagcca acgccccgag gtcttcctcc
     2221 cagaacggac tcacgtagat gttgcgcttg aacacgtcga tcggattctc gaggaagtcc
     2281 tggggcatct tcttgtagat gtcggcgagg ttcttcaaca gcggctcgac ccagctactg
     2341 ccgttctcga cgacggcaac cttgagggtc gggaaccgcg acagcgcacc gtgcacgatg
     2401 agcgcagaga ccgaatcctc gatcggacgc cactgggcga gcatgcgaaa cgcctggggt
     2461 tggaacggca ggaactcggt gtcgctgccc atccagtcgt tc
//
LOCUS       DAOOMA010000426         2488 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_51919_length_2488_cov_3.571311, whole genome shotgun sequence.
ACCESSION   DAOOMA010000426 DAOOMA010000000
VERSION     DAOOMA010000426.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 2488)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2488)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2488
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51919_length_2488_cov_3.571311"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..309)
                     /locus_tag="PLP95_10655"
     CDS             complement(<1..309)
                     /locus_tag="PLP95_10655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HPB46303.1"
                     /translation="MFARGAAGVGAIVIALSAFGGGVHTGDRPVDATEPSTAEATSTG
                     LPARVTGLRVTAAADRSVEVTWNALDDESSSGKQLAGYLIHVDWKFVKWVPKTETTTK
                     V"
     gene            complement(573..2180)
                     /locus_tag="PLP95_10660"
     CDS             complement(573..2180)
                     /locus_tag="PLP95_10660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate-forming enzyme family protein"
                     /protein_id="HPB46304.1"
                     /translation="MDQAAMDQAAIDRFQFHGQDIPWLLDHWATNTPDNDFLIWEPRS
                     GEEVRWTYRRFVDEVEKVAAALSARGVGEGDHLLIHSENSPEMVIAWYACARIGAVGV
                     TTNTRSVAAEVNYFATHTSCVGAITQPQFAALVQQAVPDIGFIVLTEDNSGEAATEAP
                     IDRETIPFADLIDHDLPAAPRLEPDALRPAGVLFTSGTTSKPKAVVHTHGNALWQAKV
                     GPRNIDMDTGDVYLAYLPFFHVNSQSWATWTTLGVGGTVVLQPKFSASRFWEVILKHE
                     VTHISLIPFVFKAVAEQPVPEHSVKVGVFGLIMPMLDAWLGMRVVAAYGMTETVTHAV
                     VSNKWEHYPDMAMGKPCPGYEMVVVDPDTKELVKPGEIGEIWLRGTRGIQLFLEYYRN
                     DEANAKAFTAEGWLQTGDLVMLGEDGNLFYRDRDKDALKVGGENVSAREVEDVCRTAG
                     PMADVAVVGKSHEMLDQVAVAFVIKGAGAPEDAEFEAAIIAACRENLADFKVPRAVYF
                     VEEFPEALLGKVAKNVLREWADAKPAL"
     gene            complement(2266..>2488)
                     /locus_tag="PLP95_10665"
     CDS             complement(2266..>2488)
                     /locus_tag="PLP95_10665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB46305.1"
                     /translation="ELRSDIDGGALLVRGRTATPGATLVVWHRDGGAVQIDTEGLGEV
                     TEHRVDGGRYLTAEVTAADYRFELVGRGA"
BASE COUNT          395 a          843 c          808 g          442 t
ORIGIN      
        1 gaccttcgtg gtggtctcgg tcttcggaac ccacttcacg aacttccagt cgacatgtat
       61 caggtatccg gcgagttgtt tcccactcga tgactcgtcg tcgagtgcgt tccacgtcac
      121 ctcgacgctg cggtcggcgg ctgcggtcac cctcagtccg gtgaccctgg ccggcaaccc
      181 cgtcgaggtc gcctcagccg tcgatggctc ggtcgcatcg accggtcggt ccccggtgtg
      241 gacaccaccg ccgaacgcgc tgagcgcgat gacgatggcg ccgacacctg cagcgccacg
      301 cgcgaacacc cgcgttcggt tcgtggatct gcccagcatg gccatgctcc ctcgtcggtg
      361 ttcgattcgc cgtgccgccc caccccagca gggtcggcct tgacgaaatg tcaccgggat
      421 cgtgacacag accgatgcat ctggctagtg cgaagtggac atctacccgc tcaggtcgtg
      481 ggcccgcatt cgccggaggc catccgagcg ctgcaagcga cgcccatctc cctccgtcac
      541 gggctccgtc gcaggttccg tcgcaggttc cgtcacaggg cgggtttggc gtccgcccac
      601 tcgcgaagca cgttcttggc gaccttgccg agcagtgcct cggggaactc ctcgacgaag
      661 tagaccgcgc gcggcacctt gaaatcggcc agattctccc ggcacgcggc gatgatcgcc
      721 gcttcgaact cggcatcctc gggcgccccg gcgcccttga tcacgaacgc gaccgcaacc
      781 tggtcgagca tctcgtgcga cttgccgacc actgcgacat cggccatcgg cccggcggtg
      841 cgacacacgt cttcgacctc tcgggccgac acgttctcgc caccgacctt gagggcgtcc
      901 ttgtcgcgat cgcgatagaa gaggttcccg tcctcgccga gcatcacgag atcgccggtc
      961 tgcagccacc cctccgcggt gaaggccttc gcgttggcct cgtcgttgcg gtagtactcg
     1021 aggaacagtt gaatcccccg ggtgccgcgc agccagatct caccgatctc gcccggtttc
     1081 acgagttcct tggtgtcggg gtcgacgacg accatctcgt agccggggca cggcttgccc
     1141 atcgccatgt cggggtagtg ctcccacttg ttcgacacga ccgcgtgggt gacggtttcg
     1201 gtcatgccgt aggcggcgac gacgcgcatg ccgagccagg cgtcgagcat cggcatgatg
     1261 aggccgaaca cgccgacctt aacgctgtgt tcgggcacgg gctgctcggc gacggccttg
     1321 aacacgaacg ggatcagcga gatgtgggtg acctcgtgct tcaggatgac ctcccagaag
     1381 cggctcgctg agaacttcgg ctgcaacacc acggtgccgc cgacgccgag cgtcgtccag
     1441 gtcgcccagc tctggctgtt cacgtggaag aacggcaggt aggcgaggta gacatcgccg
     1501 gtgtccatgt cgatgttgcg cgggccgacc ttggcctgcc acagcgcgtt gccgtgggtg
     1561 tgcacgacgg ccttgggctt ggaggtcgtg ccggaggtga acaacacgcc ggccgggcgc
     1621 agcgcgtcgg gttcgaggcg gggcgctgcg ggaagatcgt ggtcgatcag gtcggcgaag
     1681 gggatcgtct cgcggtcgat cggcgcctcg gtggccgctt cgccggagtt gtcctcggtg
     1741 agcacgatga acccgatgtc gggcacggcc tgttgtacga gcgcggcgaa ctgcggctgg
     1801 gtgatggcgc cgacgcagct cgtgtgggtg gcgaagtagt tgacctcggc cgcaaccgag
     1861 cgggtgttag tcgtcacacc gacggcgccg atgcgggcac acgcgtacca ggcgatgacc
     1921 atctcggggc tgttctcgga atggatgagg aggtgatctc cctcaccgac acccctggcc
     1981 gacagcgcgg ccgcgacctt ttccacctcg tcgacgaagc ggcgatacgt ccagcggacc
     2041 tcctcacccg agcggggctc ccagatcagg aagtcgttgt cgggcgtgtt ggtcgcccag
     2101 tgatcgagca gccacggaat gtcttgtccg tggaactgaa accggtcgat cgcggcctga
     2161 tccatcgctg cctgatccat caaagagccc ccttcggcgt gtggccctac agcatgccaa
     2221 agccgccgag gcagcgcatc atcgaacgtt cgttcaggcg ccgggtcagg cgccgcggcc
     2281 gaccaattca aagcggtagt cggcggcggt cacctcggca gtgaggtagc gcccgccgtc
     2341 gactcggtgc tcggtcacct cacccagacc ctcggtatcg atctgcacgg caccaccgtc
     2401 gcgatgccac accaccaacg ttgcgccagg cgtcgcagtg cgcccccgca cgagcagcgc
     2461 tccgccatcg atgtcgcttc gcagctcg
//
LOCUS       DAOOMA010000427         2486 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_51983_length_2486_cov_3.200740, whole genome shotgun sequence.
ACCESSION   DAOOMA010000427 DAOOMA010000000
VERSION     DAOOMA010000427.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 2486)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2486)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2486
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51983_length_2486_cov_3.200740"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            4..513
                     /gene="ilvN"
                     /locus_tag="PLP95_10670"
     CDS             4..513
                     /gene="ilvN"
                     /locus_tag="PLP95_10670"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990610 - acetolactate synthase regulator
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HPB46306.1"
                     /translation="MSAPNAPKHHMLSVLVENKAGVLARVASLFARRGYNIYSLAVAP
                     TDDPAYSRIAIVADADSAPLEQIVKQLNKLINVIKISELDPNDAVEQELMVLTVSADS
                     ATRSQIVELANIFGARIIDVGHSDVTISAAEVPSRLDDLEDLLRPYGIVVVQRTGRIA
                     LPRLDRSRL"
     gene            626..1657
                     /gene="ilvC"
                     /locus_tag="PLP95_10675"
     CDS             626..1657
                     /gene="ilvC"
                     /locus_tag="PLP95_10675"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012230278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HPB46307.1"
                     /translation="MANVFYEKDADRSLILGRKVAILGYGSQGHAHALNLKDSGVEVV
                     VGLRDGSSSKAKAEAAGLTVMGLAEASAWADLIMILLPDTEQKDIYDEFIAPNLNAGD
                     ALFFAHGFNIRYEQIVPPTDVDVALVAPKGPGHLVRRTYTEGGGVPSLIAVHQDATGN
                     ARALAMAYADANGGTRAGVLETTFPEETETDLFGEQVVLCGGLTSLIKAGFETLVEAG
                     YQPEAAYFECLHEVKLIVDLIYEEGIEGMRYSISDTAEYGDLTRGPRIITPAVKDEMR
                     NVLAEIQDGRFADEWIKESRSGRANFHRLEEEGKTHQIEKVGKELRALMPWISQGKQS
                     VKDAAGGQS"
     gene            complement(1755..>2486)
                     /locus_tag="PLP95_10680"
     CDS             complement(1755..>2486)
                     /locus_tag="PLP95_10680"
                     /inference="COORDINATES: protein motif:HMM:NF019581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HPB46308.1"
                     /translation="GSNQAGTGYQREPYLNSTSRGELNFTVEQLAATIDRCHRGGWQI
                     GVHANGDAAIDTTIDAYEAALEATPRPDHRHRIEHCSVLHPEQIERMARLGLSPSFLI
                     GHIRWWGEAFRDRLLGPERSMHYDPCASALAAGLRISLHSDYNVTPLEPLRYVEDAVN
                     RIMAANGEVLNDAERISVEAGLRAVTSDAAWQLREDHRAGTIEVGKSADLCVLDDDPF
                     RVDATRISRIAVSATYRDGVLRHGA"
BASE COUNT          425 a          828 c          797 g          436 t
ORIGIN      
        1 ccagtgagcg caccaaacgc acccaagcac cacatgttgt cggtgctcgt cgagaacaag
       61 gcgggggtgt tggcccgggt ggcgagcctg ttcgcccgcc gcgggtacaa catctactcc
      121 ctcgcggtcg ccccgaccga cgatccggcc tacagccgca tcgccatcgt ggcggatgcc
      181 gacagcgccc cgctcgagca gatcgtcaag cagctcaaca agttgatcaa cgtcatcaag
      241 atctccgaac tcgacccgaa tgacgccgtc gaacaggagt tgatggtgtt gacggtgagc
      301 gcggattcgg cgacccgctc gcagatcgtc gagttggcga acattttcgg ggcccggatc
      361 atcgacgtcg gccattccga cgtcaccatt tcggccgccg aggtcccgtc tcgcctcgac
      421 gatctcgaag atctgttgcg tccctacggg atcgtcgtgg tccagcgcac cggccgcatc
      481 gcccttccgc gactcgatcg cagccggctc tgaccggcac ccgtcatcgg cgtcccgacg
      541 tcgaaatctg catcgtcgta tttccgaacc cccgctcgtg ccagtagttt cggcacgctc
      601 gaacacccga aaggtaccac cctccatggc caacgttttc tacgagaagg acgccgaccg
      661 ttccctcatc ctgggtcgca aggtcgccat cctcggctac ggctcccagg gccacgctca
      721 cgcgctcaac ctcaaggact ccggcgtgga ggtcgtggtc ggccttcgtg acgggtcatc
      781 gtcgaaggcc aaggccgagg ccgccggtct caccgtgatg gggctcgccg aggcgtcggc
      841 gtgggccgac ctgatcatga tcctgttgcc cgacaccgag caaaaggaca tctacgacga
      901 gttcatcgcc ccgaacctga acgccggcga tgcgctcttt ttcgcccacg gcttcaacat
      961 ccgttacgaa cagatcgtgc cgccgaccga cgtcgacgtc gcgctcgtcg ccccgaaggg
     1021 ccccggccat ctcgttcgac gcacctacac cgagggtggc ggtgtgccgt cgctcatcgc
     1081 cgttcaccag gacgcgaccg gcaacgcccg tgcgctcgcc atggcctatg ccgacgccaa
     1141 cggcggcacc cgcgccggtg tgctcgaaac caccttcccc gaggagaccg aaaccgatct
     1201 cttcggcgag caggtcgtgt tgtgcggtgg tctgacctcg ctcatcaagg ccgggttcga
     1261 gaccctcgtc gaggccgggt atcagcccga ggcggcgtat ttcgaatgcc tgcacgaggt
     1321 gaagctcatc gtggatctca tctacgaaga gggcatcgaa ggcatgcgct actcgatctc
     1381 cgacaccgcc gagtacggcg acctgacccg cggcccgcgc atcatcaccc cggcggtgaa
     1441 agacgagatg cgcaacgtgt tggccgagat ccaggacggc cgcttcgccg acgagtggat
     1501 caaggagtcg cgttcgggtc gggcgaactt ccaccgcctc gaggaagagg gcaagaccca
     1561 tcagatcgaa aaggtcggca aggagcttcg tgcgctgatg ccgtggatct cccagggcaa
     1621 gcagtcggtg aaggacgccg ctggcggtca gtcctgacgc tcgaagcctg acgctcgaag
     1681 atcgtcgctg ttcatccggc tggagttggc gcttggcgtc gcctccagcc ggatgattcg
     1741 cgtccggggg cgcgtcacgc gccgtggcgc aacaccccgt cgcggtaggt ggcgctgacg
     1801 gcgatgcgtg aaatgcgcgt ggcgtccact cggaacggat cgtcgtcgag gacacacagg
     1861 tcggccgatt tgcccacctc gatcgtgccg gcgcggtgat cctctcgaag ctgccaggcg
     1921 gcgtcgctgg tgaccgcacg cagcccggcc tcgacgctga tgcgttcggc atcgttgagc
     1981 acttcaccgt tggctgccat gatccggttg acggcatctt ccacgtaacg cagcggctcg
     2041 agcggggtca cgttgtagtc ggagtgcagc gagatgcgca ggcccgcagc gagcgctgag
     2101 gcacacgggt cgtagtgcat cgatcgttcg gggccgagga gccggtctcg gaacgcctcg
     2161 ccccaccagc ggatgtgtcc gatgagaaac gacggggaca gccccagcct cgccatgcgt
     2221 tcgatctgtt cggggtgcaa gacgctgcag tgctcgatgc gatggcggtg atccgggcgc
     2281 ggcgtcgctt caagcgcggc ctcatatgcg tcgatcgtgg tgtcgatcgc ggcatcgccg
     2341 ttggcgtgca ccccgatctg ccagccgccg cggtgacagc gatcgatggt ggccgcgagt
     2401 tgttcgacgg tgaagttcag ctcgcctcgc gaggtcgaat tcaggtaggg ctcgcgctgg
     2461 tagccggtgc cggcctgatt cgatcc
//
LOCUS       DAOOMA010000428         2480 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_52174_length_2480_cov_3.365361, whole genome shotgun sequence.
ACCESSION   DAOOMA010000428 DAOOMA010000000
VERSION     DAOOMA010000428.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 2480)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2480)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2480
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52174_length_2480_cov_3.365361"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..195)
                     /locus_tag="PLP95_10685"
     CDS             complement(<1..195)
                     /locus_tag="PLP95_10685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB46309.1"
                     /translation="MAFSPRTSRALLALSILTLGMAMPSIAFAEVIESSVEQLAVQPG
                     TELVGLDDFTMIAVSWTGATT"
     gene            194..1414
                     /locus_tag="PLP95_10690"
     CDS             194..1414
                     /locus_tag="PLP95_10690"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012222741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase"
                     /protein_id="HPB46310.1"
                     /translation="MALTVSKELRNAWSATAPAAGWAAGWGRVGSKVLRLYAVKGLIL
                     SGGAGTRLRPITHTSAKQLVPVANKPILFYGIDDMVEAGITEIGIITGETGAEVREAV
                     GDGSQFGANVTYIPQDEPLGLAHCVLIAKDFLGDDDFVMYLGDNMLQQGLVEFVARFN
                     DDKRAANEPTLDGSVVPPAAQILLCEVPDPHRFGIAAVDDEGRVVRLVEKPADPPSNL
                     ALVGVYLFTKLIHNAVAAIKPSPRGELEITDAIQWLIESGQRVRHDLLEGWWLDTGKK
                     DPLLESNRRVLEELEPSVEGEVDDASEIDGRVSIEAGAKLINSHVRGPAIIGAGTVLT
                     NAYVGPFTSVAANCTITDSEIENSVVLDNAVIENVPRLVDSLLGRNTTVHRTAQRPKA
                     TRLMIGDDCTIDLE"
     gene            1422..1988
                     /locus_tag="PLP95_10695"
     CDS             1422..1988
                     /locus_tag="PLP95_10695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015797996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase family
                     protein"
                     /protein_id="HPB46311.1"
                     /translation="MATVTESDVIKGVYVVEPTKFGDERGYFIETYRREWFPQGREMI
                     QGNRGNRIAGCLVGLHYHLHQADYWYVPFGHARVVLHDLRQGSPTDGATLQFDLGEVD
                     GGENNHLGIFIPPGVAHGFASITDMAITYLVDGYYNPKDECGVAWNDPAVEADWGVAD
                     PILSQRDQQNPLRSELDPMFQPHYSLRT"
     gene            2000..>2480
                     /locus_tag="PLP95_10700"
     CDS             2000..>2480
                     /locus_tag="PLP95_10700"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020378318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HPB46312.1"
                     /translation="MKLFVTGAAGFIGSNFARHILTTSDDEVVVFDALTYAGNRESLA
                     DLEDNPRFSFVHGNICDRDAVRDAMQGCDQVVHFAAESHVDRSLLDPDVFVRTNCDGT
                     NVMCDIATQLGVERFLHISTDEVYGSIEEGSFVEGDPLMPRSPYSAAKAGSDLIAMAY
                     "
BASE COUNT          461 a          823 c          761 g          435 t
ORIGIN      
        1 ggtggtcgcg ccggtccagc tgacggcgat catcgtgaag tcgtccaggc cgaccaattc
       61 ggttcccggc tgcaccgcca gctgctcgac cgacgattcg atgacttcgg cgaacgcgat
      121 tgatggcatt gccataccga gcgtgagaat cgacagcgcc agcagggcgc gactggtccg
      181 tggggaaaag gccatggcat tgacggtatc gaaggaactc cgcaacgcgt ggtcggcgac
      241 ggccccggcg gcagggtggg cggcggggtg gggtcgcgtt ggttcgaagg tgctaagact
      301 gtatgccgtg aagggtctga tcctgtcggg cggtgcggga acgcgcctgc gccccattac
      361 ccatacgagc gccaagcaac tcgttccggt cgccaacaag ccgattctgt tctacggcat
      421 tgatgacatg gtcgaggccg ggattaccga gatcggcatc attaccggcg aaacgggagc
      481 cgaggtccgc gaggcggtcg gtgacggatc gcagttcggc gccaacgtca cctacatccc
      541 ccaggacgag ccgctcggcc tcgctcactg cgtgctgatc gctaaggatt ttctcggcga
      601 cgacgacttc gtgatgtatc tcggcgacaa catgttgcag cagggactcg tcgagtttgt
      661 cgcccggttc aacgacgaca agcgcgccgc caacgagccg accctcgacg gctcggtcgt
      721 gccaccggcc gcacagattc ttctgtgcga ggttcccgac ccgcatcggt tcggaatcgc
      781 cgcggtcgac gacgagggtc gggtcgtccg cctggtcgaa aaaccggccg atccgccctc
      841 caacctggca ctcgtcggcg tctacctctt caccaagctc atccacaacg cggtcgccgc
      901 aatcaagccg tcgcctcgtg gggaactcga gatcaccgac gccatccagt ggctcatcga
      961 atcgggccaa cgtgttcgcc acgacctgct cgaggggtgg tggctcgaca ccggtaagaa
     1021 ggacccgctg ctcgaatcga accgacgtgt cctcgaggaa ctcgagccca gcgtcgaggg
     1081 cgaggtcgac gacgcgtcgg aaatcgacgg tcgcgtgagc atcgaagcag gcgcgaagct
     1141 catcaacagt catgtgcgcg gcccggcgat catcggtgct ggcacggtgc tgacgaatgc
     1201 gtatgtcggc ccattcacct ccgtcgctgc caactgcacg atcaccgatt ctgagatcga
     1261 gaactcggtg gtcctcgaca acgcggtcat cgaaaacgtg ccgcgcctgg tcgactcgct
     1321 gctcggccgc aacaccaccg ttcaccgcac cgcgcagcgt cccaaggcaa cccgcctgat
     1381 gatcggcgac gactgcacca tcgacctgga gtaatcacct catggcaacc gtgaccgagt
     1441 ccgacgtgat taagggcgtc tacgtcgtcg aacccaccaa gttcggcgac gagcgcggct
     1501 atttcatcga gacctaccgc cgcgagtggt tcccccaggg ccgcgaaatg atccagggca
     1561 accgtggaaa tcgcatcgcc gggtgcctcg tcggtctcca ctaccacctg caccaggccg
     1621 actactggta cgtcccgttc ggccacgccc gcgtcgtatt gcacgacctt cgccaggggt
     1681 cgcccaccga tggcgcaacc ctgcagttcg acctcggcga ggtggatggc ggcgagaaca
     1741 accacctcgg tatcttcatc ccgccgggcg tcgcccacgg gttcgcctcg atcaccgaca
     1801 tggcgatcac ctacctcgtc gacgggtatt acaacccgaa agacgaatgc ggtgtggcgt
     1861 ggaatgaccc ggcggtcgag gccgactggg gggtcgccga tccgattctg tcgcagcgcg
     1921 accagcagaa cccgctgcgc agcgaactcg accccatgtt ccaaccccac tattcgctcc
     1981 gtacctgagg gatcctcacg tgaagctttt cgtcaccggt gccgccggtt tcatcggttc
     2041 caacttcgcc cgccacatcc tgaccacctc cgatgatgag gtcgtcgtct tcgacgcgtt
     2101 gacctatgcg ggcaatcgcg agagcctcgc cgaccttgag gacaatccgc ggttcagctt
     2161 cgtgcacggc aacatctgcg atcgcgacgc ggtccgcgac gcgatgcagg gatgcgacca
     2221 ggtggtgcac ttcgccgcag agagccacgt cgatcgctcg ctgctcgacc ccgacgtatt
     2281 cgtgcggacc aattgcgacg gcaccaacgt gatgtgcgac atcgcgacac aactcggtgt
     2341 cgagcggttc ctgcacatct ccaccgacga ggtgtacggc tcgatcgagg agggcagctt
     2401 cgtcgagggg gatccgctga tgccgcgctc gccgtactct gcggccaagg ctggctcgga
     2461 tctcatcgcc atggcctatc
//
LOCUS       DAOOMA010000429         2476 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_52290_length_2476_cov_3.743081, whole genome shotgun sequence.
ACCESSION   DAOOMA010000429 DAOOMA010000000
VERSION     DAOOMA010000429.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 2476)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2476)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2476
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52290_length_2476_cov_3.743081"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(5..400)
                     /locus_tag="PLP95_10705"
     CDS             complement(5..400)
                     /locus_tag="PLP95_10705"
                     /inference="COORDINATES: protein motif:HMM:NF015279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB46313.1"
                     /translation="MDRLGVADERGVHQRGDALHLKRHPAHAAERWQPQVMQLSALKA
                     QGIADFWAAVTSFETKRRASGEFDARRRHQAQDWMWDIVHAGLRHAFDRHPAVRAALP
                     QTLRDVDEARAAPSAAARALLNLFQKPNS"
     gene            329..526
                     /locus_tag="PLP95_10710"
     CDS             329..526
                     /locus_tag="PLP95_10710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB46314.1"
                     /translation="MTFQVEGVTTLVYSPLVGDTETVDECDFVSLLPDPASLPDREDA
                     TRSQRELIDRIEQASDGCDDG"
     gene            536..2065
                     /gene="glpK"
                     /locus_tag="PLP95_10715"
     CDS             536..2065
                     /gene="glpK"
                     /locus_tag="PLP95_10715"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015798310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HPB46315.1"
                     /translation="MAVVLSIDAGTTGVRSVAVDERGAVTNWHYREFTQHFPRPGWVE
                     HDAAEIWSAIEATMTDLVAELDEPIAAIGITDQRETIVAWDRRTGAPLSRAIVWQDRR
                     GAARCEELAELGHLDLVRSTTGLVLDPYFSATKIEWLIRTGGVEVTADTAFGTIDSWI
                     LWNLTGGRADRGAVHATDTSNASRTMLFDIASGAWSPELCDLFGVPMVSLPDVMASAG
                     RFGVTGTDLPVEAGIPISGIAGDQQAALFGQACVRPGMTKNTYGTGSFVLMNVGDTLP
                     TPADGLLTTVAWTLPGRLLGNEGPELVTHYALEGAIFATGAAVQWLRDGLQIIDSAAQ
                     AGPIAESVPDTGGVVVVPAFTGLGAPYWDPYARGTIVGLTRGSTRAHLVRAVVESMAY
                     QTRDVVEAMASASGRSISELRVDGGASQMNLLLQLVADQLGVEVRRPVVKETTALGAA
                     YLAGLAEGVWATVDDLDGAWIEDARVGPGDGRTEADERYRMWTRAVERSRDWADADEP
                     D"
     gene            complement(2062..>2476)
                     /locus_tag="PLP95_10720"
     CDS             complement(2062..>2476)
                     /locus_tag="PLP95_10720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB46316.1"
                     /translation="TAALFGADWVAAVSMARPPAVVSSVGDDLPSAEWMAAFAFGAAS
                     GADTEPSGVSPRAATGGGVVIGELACASLGESVDHGRDGLELLLSRRDLPLREVERSL
                     LERLSSLARTRLAALPSATVPSATVPSATVPSADA"
BASE COUNT          396 a          876 c          847 g          357 t
ORIGIN      
        1 ctcctcagga attcggtttc tggaacaggt tcaggagcgc gcgcgccgcc gccgaaggcg
       61 cggcacgcgc ctcgtcgacg tcgcgcaggg tctgcggcag cgcggcacgc accgcggggt
      121 gccggtcgaa ggcgtggcgc aggccggcgt ggacgatgtc ccacatccag tcctgcgcct
      181 ggtgacggcg ccgcgcgtcg aactcgccgc tggcacggcg cttcgtctcg aagctcgtca
      241 cggccgccca gaagtcggcg atgccctgcg ccttcagcgc gctgagctgc atcacctgcg
      301 gctgccagcg ctcagcggcg tgcgccgggt gacgtttcag gtggagggcg tcaccacgct
      361 ggtgtactcc ccgctcgtcg gcgacaccga gacggtcgat gaatgcgact tcgtctccct
      421 gttgcccgat ccggcgtcgc tgcccgaccg cgaggacgcc acccgatccc aacgcgagct
      481 catcgaccgg atcgaacagg cgagcgacgg ctgcgacgac gggtagattc acgccatggc
      541 cgtcgtgttg tccatcgatg caggtaccac cggggtgcga tccgtcgccg tcgacgagcg
      601 cggcgcggtg accaactggc actaccgaga gttcacccag cactttcctc gtcccgggtg
      661 ggttgaacac gatgccgccg aaatctggtc ggcgatcgag gcgacgatga ccgacctggt
      721 cgccgaactc gacgaaccga tcgccgcgat cggcatcacc gaccaacgcg aaacgatcgt
      781 cgcctgggat cggcgcacgg gggcaccgct gagccgggcg atcgtgtggc aggatcgccg
      841 gggagccgcc cgttgcgagg aactcgccga actcggccac ctcgacctgg tgcgatcgac
      901 caccgggctg gtgctcgatc cgtacttctc ggcgaccaag atcgagtggt tgattcgaac
      961 cggcggcgtc gaggtgaccg cggacaccgc cttcggaacg atcgattcat ggatcctctg
     1021 gaacctcacc ggcggccgcg ccgaccgcgg ggcggtgcat gccaccgaca cctccaacgc
     1081 gagccgcacg atgctgttcg acatcgcctc cggagcctgg tccccggaac tgtgcgacct
     1141 gttcggcgtg cccatggtgt cgctgcccga cgtcatggcc tccgccggac gcttcggcgt
     1201 caccggaacc gacttgccgg tcgaagccgg catccccatc tccggaatcg ccggcgacca
     1261 acaggccgcg ctgttcggcc aggcgtgcgt acgccccggg atgaccaaga acacctacgg
     1321 caccggctcg ttcgtgctca tgaacgtcgg cgacacactc cccacccccg ccgatggcct
     1381 cctcacgacc gtggcgtgga ccctgcccgg acgactgctc ggaaacgagg gcccggaatt
     1441 ggtgacccat tacgcgctcg agggcgcaat cttcgccacc ggcgcagcgg ttcagtggct
     1501 gcgcgacggc ttgcagatca tcgactcggc cgcacaagcg ggtccgatcg ccgagtcggt
     1561 ccccgacacc ggcggagtcg tcgtcgtccc ggcgttcacc ggtctgggtg cgccctactg
     1621 ggacccgtac gcccgcggaa ccatcgtcgg gctcaccaga gggtcgaccc gtgctcatct
     1681 ggtgcgcgcc gtcgtcgagt cgatggccta tcagacccgc gacgtcgtcg aggcgatggc
     1741 ctcggccagc ggtcgctcga tcagcgagct ccgcgtcgac ggcggggcgt cgcagatgaa
     1801 cctgctgctg caactcgtcg ccgatcaact cggcgtcgag gttcgacgcc cggtggtgaa
     1861 ggagacgacc gcactcggcg ccgcatacct cgccggcctg gccgaagggg tctgggcgac
     1921 ggtcgatgac ctcgacgggg cgtggatcga agatgcccga gtcggccccg gcgacggacg
     1981 caccgaggcc gacgagcgtt accgaatgtg gacgcgggcg gtcgagcggt cgcgagattg
     2041 ggccgacgcc gacgaaccgg attaggcgtc ggctgagggc accgtcgctg agggcactgt
     2101 cgctgagggc actgtcgctg agggcaatgc ggcgagccgc gtgcgggcga gcgagctgag
     2161 ccgctcgaga agcgaacgtt cgacctcacg caacggtagg tcgcggcgcg acagcaacag
     2221 ttcaagtccg tcgcgcccgt gatcgaccga ctccccgagc gaggcgcagg cgagttcgcc
     2281 gatcaccacc ccgccccccg tcgcagcccg cggtgagacg ccggacggct cggtatccgc
     2341 accggaggca gcgccgaacg cgaacgccgc catccactcg gccgacggga ggtcgtcgcc
     2401 aacgctcgag acgactgccg gaggccgggc catcgacacc gccgcgaccc agtcggcccc
     2461 gaacagcgct gcggtg
//
LOCUS       DAOOMA010000430         2466 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_52592_length_2466_cov_5.481543, whole genome shotgun sequence.
ACCESSION   DAOOMA010000430 DAOOMA010000000
VERSION     DAOOMA010000430.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 2466)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2466)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2466
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52592_length_2466_cov_5.481543"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>796)
                     /locus_tag="PLP95_10725"
     CDS             complement(<1..>796)
                     /locus_tag="PLP95_10725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB46317.1"
                     /translation="LPAAEIKLEADLKGLGADLVERIGEAASEAGAVFAEALLSAIAS
                     VSVPPLEVTADTSGFASSLGDFAPIDVPIHTDTEAATAEIEALLASLASEAATIELDA
                     DSAAALSAIADVVDNIPGVDIELNVDTSAFDAAIEDALGDAPELEIGVTADTSSLDGL
                     TSSLDSESVVIGVEADTSAAASEIDALRGSVEGEPVKMSVTGTESANANLGQLVGLAQ
                     SAKGGISGVGNELAKIPGVATPAGAAIAGIGVAAAVSFGAARDAAVV"
     assembly_gap    797..806
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(859..1191)
                     /locus_tag="PLP95_10730"
     CDS             complement(859..1191)
                     /locus_tag="PLP95_10730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB46318.1"
                     /translation="MPLADVLDAVERFAVEEHLDLVEHAIAHPLANPSTKKALDGADN
                     MRRWAEILDGPRNAVSAWLADDPTKPAVVEVSENEVALMARSWRVSRDEARARLLAMA
                     EGGADADR"
     gene            complement(1197..1619)
                     /locus_tag="PLP95_10735"
     CDS             complement(1197..1619)
                     /locus_tag="PLP95_10735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB46319.1"
                     /translation="MSTNVIDITSQLERRKLTPVAIGFGDEVFQFEPVIDFVALQDKD
                     TAIDWYFGWLSDHLVERTREELHDALVKLSRERGMQLGDVLHVMVNAYIKAAREQVAA
                     AVEAAAERPTKPRSGSGRSTTRGARKSAAGSSLVPVGR"
     gene            complement(1630..2022)
                     /locus_tag="PLP95_10740"
     CDS             complement(1630..2022)
                     /locus_tag="PLP95_10740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB46320.1"
                     /translation="MSVQINEAELARLFGVGGPVHAKFATYARRIQTTAQQLNPTNAR
                     TGAYRGGFFNESRGGGTDWSYAIGNTAPHSTFIERGTRPHTIRPRRGKFLVFQGADGR
                     TVFAREVHHPGTEAHRVLQRALETTPYR"
     gene            complement(2019..>2466)
                     /locus_tag="PLP95_10745"
     CDS             complement(2019..>2466)
                     /locus_tag="PLP95_10745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB46321.1"
                     /translation="TISHCRPLGCCVDLRVYLDPLSVFDPNANPFDTSSCCAAVEYWR
                     LWVEYLRPWCAHVRLDRNPTAEEYTAATNDVLGCFDDFRCAWRKALQSGLILPTKDGG
                     SCVSPDAIFWQPPEMLCPDGCGEAESGVAAGWRVGALIRYGARRPA"
BASE COUNT          441 a          786 c          828 g          401 t
ORIGIN      
        1 acaacagcgg cgtcacgagc cgcgccgaac gacaccgcgg cagcaacacc aataccggca
       61 atggcagcac ccgcgggggt ggcgacgccg gggatcttgg ccaactcgtt tcccaccccg
      121 gaaatgccgc ccttggccga ttgcgctagg ccgacgagtt ggccgaggtt ggcgttggcg
      181 gactcggtgc cggtaaccga catcttcacg ggttcgccct cgaccgagcc gcgtagggcg
      241 tcgatctctg acgcagccgc cgacgtatcg gcttccacac cgatcacaac cgactcggag
      301 tcaagcgaac tcgtgaggcc gtcgagtgaa ctggtgtcgg cggtaacgcc gatttcgagt
      361 tcgggcgcat caccgagcgc gtcctcgatg gcagcatcga acgccgacgt gtcgacgttc
      421 aactcgatgt cgacgccggg aatgttgtcg accacgtccg cgatggcact gagtgcagcg
      481 gcggagtccg cgtcgagttc gatcgttgcg gcttcggacg ccaacgacgc cagtagtgct
      541 tcgatctcgg cggtagcggc ttcggtgtcg gtgtggatcg gaacgtcgat cggagcgaag
      601 tcacccagcg acgaggcgaa accgctcgtg tcggcagtga cttcgagcgg cggaaccgac
      661 acggacgcga tggccgagag caacgcttcg gcgaacaccg cgcccgcctc cgacgcggct
      721 tctccgatgc gctcgacaag atcggcaccg agccccttga ggtcggcctc caacttgatc
      781 tcggccgcgg gcaggtnnnn nnnnnncgtg agttcggagg agaaccgcga gaagtccggc
      841 cggatgacaa caacgtcctc agcgatcggc atcggcaccc ccttcagcca tcgccaacaa
      901 ccgcgcccgc gcttcgtcgc gagagacacg ccacgaccgc gccatgagcg cgacctcgtt
      961 ctcggacacc tcgacgacag cgggcttggt ggggtcatcg gcaagccacg cgctcacggc
     1021 gttgcgcgga ccgtcaagaa tctcggccca acgacgcatg ttgtcggcgc cgtctagcgc
     1081 ctttttcgtc gacgggttgg ccagcggatg cgcgatggcg tgctcgacaa ggtcgaggtg
     1141 ttcctcgact gcgaaccgtt caacggcatc gagcacgtcc gcgagaggca tggccctcag
     1201 cgaccgaccg gtacgagcga tgaaccagcc gcggacttcc gagccccgcg agttgtagag
     1261 cgaccagagc cggaccgcgg cttggtaggg cgctcagccg cggcctccac ggcggcggcg
     1321 acctgttcac gagcggcctt gatgtaggca ttaaccatca cgtggagaac gtcaccgagc
     1381 tgcatccccc gctcgcgtga gagcttcacc agggcgtcgt gcagttcctc gcgggtccgc
     1441 tcgacaaggt gatcggacag ccagccgaag taccagtcga tcgcggtgtc tttgtcctgc
     1501 aaggcgacga agtcgatcac cggctcgaac tggaacacct cgtcgccaaa gccgatcgcg
     1561 accggcgtga gcttgcggcg ctctagctgt gaggtgatgt caatcacgtt tgtgctcatt
     1621 tgtgggctcc tatcggtagg gggtggtttc cagcgctcgc tggagaactc ggtgcgcttc
     1681 ggtgcccggg tggtgcacct ctcgcgcaaa aacagtgcgg ccgtccgcgc cttgaaaaac
     1741 aaggaacttc ccgcggcggg ggcggatcgt gtgcggtcgc gtgccgcgct cgatgaacgt
     1801 cgagtgcggg gctgtgttgc cgatcgcgta ggaccagtcg gtgccgccgc cgcgtgactc
     1861 gttgaagaac ccgccgcggt aggcgcccgt gcgcgcgttg gttgggttca gttgctgggc
     1921 cgtggtctga atgcgccgcg cgtaggtcgc gaacttggcg tggaccggac cgccgacgcc
     1981 gaacagtcga gcgagttcgg cctcgttgat ctgcacactc atgcgggcct ccgtgctccg
     2041 taccgaataa gggcgccgac gcgccagccc gcggcgacac ccgattcggc ctcgccgcag
     2101 ccgtcggggc acaacatttc gggcggttgc cagaagatgg cgtcgggcga gacgcatgaa
     2161 ccgccgtcct tggtggggag gatcaagccc gactgaagcg ccttgcgcca cgcgcagcga
     2221 aagtcgtcaa agcagccgag cacgtcgttg gtcgccgcgg tgtactcctc cgcagtgggg
     2281 ttgcgatcaa ggcgcacgtg cgcgcaccac gggcgcaagt attcgaccca gaggcgccag
     2341 tactccactg cagcgcaaca cgacgaggtg tcgaaagggt tcgcgttcgg atcgaacacc
     2401 gagagcgggt cgagataaac gcgaaggtca acgcagcagc cgaggggccg acagtgcgaa
     2461 atggtg
//
LOCUS       DAOOMA010000431         2466 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_52605_length_2466_cov_3.729573, whole genome shotgun sequence.
ACCESSION   DAOOMA010000431 DAOOMA010000000
VERSION     DAOOMA010000431.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 2466)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2466)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2466
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52605_length_2466_cov_3.729573"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            287..2302
                     /gene="dnaX"
                     /locus_tag="PLP95_10750"
     CDS             287..2302
                     /gene="dnaX"
                     /locus_tag="PLP95_10750"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR02397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HPB46322.1"
                     /translation="MAHQSLYRRYRPRRFADVRGQGHVIKALRNAVAQGTEGHAYLFS
                     GPRGTGKTSTARILAKALNCPNLVDGEPCVECDSCRSIENGSSYDLFELDAASNNGVD
                     AMRDLISRTVVGSPGRTKVYILDEVHMLSPAASNALLKTLEEPPDHVVFVLATTDPQK
                     VLPTIRSRTQHFEFSLLSANELADYVRWIGTDAGLDIDETAVEHVVRHGRGSARDTLS
                     ALDMVVAAGGVSDRADSVDEIVAALSGRDTGRVMSAIADAVAQGREPRVLAENLLTTL
                     RDAFLTSVHATLDHLSDADQTKMAETAAALGTATITRALESIGAAIVEMRQAADPRVP
                     LEVALIRLTTPSASADLDDLLQRIEDLERRVARGAGSAGPAAIDADASDQRANTPIDS
                     PNDAPRGAPSAPAAPQRPVAPGAASSPADIRAQLAARKNPGGPTSAPPIPSRRATGAA
                     GAAGAAGDDPATRRPPEPPQAAAAVSAPPTVAAEESDRPQTVAGSGAPVTLEQLAAVM
                     AESVIPTLGGMNKSLFNAGRFVGERDGMWEFALDNAPERVVSRAQGAIGDIEAILASR
                     LGVAVRVHLTSTTAGASPQAIRRDVGPASTPAAPASQSSGERSAAGPPATTAAVSPED
                     AAPHDEDDDEYVDIDVHELEDANDTAISGLDKVIAAFPGAVVMEEDQ"
BASE COUNT          421 a          844 c          836 g          365 t
ORIGIN      
        1 catcgttcgg gggcctcgtc gggatccgac accgcgttcg gtgggatcgg ggaccaggtg
       61 gcggccggat cgatccagcg gtacccatcc caccagagcc accggccgac gcctggctgg
      121 aagtgccacc cgggcggggc gactggcggg gatccgggca tcgacatgtc accaggatac
      181 ggacggggag tcggcggtcc gatcgcgagc ctgcgtgccg tgcatgattc gagtcgtcca
      241 ctggccaccg cctgctcacc ggtgcggacc ccggtagatt gtcgtgatgg cccatcagtc
      301 gctctatcgc cggtatcgac cgcgtcgctt cgccgatgtt cgcggccagg gccatgtcat
      361 caaggcactg cgaaacgcgg tcgctcaggg caccgaaggc catgcctacc tgttctcggg
      421 gccccgcgga accggcaaga cctcgaccgc gcggatcctc gccaaggcgc tgaattgtcc
      481 caacctcgtc gacggtgagc cgtgtgtgga gtgcgacagc tgccgctcga tcgaaaatgg
      541 ctcgagctac gacctgttcg aactcgacgc tgcgtcgaac aacggcgtcg atgcgatgcg
      601 cgacctgatc agccgaacgg tcgtggggtc gccggggcgg accaaggtgt acatcctcga
      661 cgaggtgcac atgttgtcgc cggcggcgtc gaacgcattg ctcaaaacac tcgaagagcc
      721 gccggatcac gtcgtgttcg ttctcgccac gaccgatccg caaaaagtgc tcccgaccat
      781 tcgcagccga acccagcatt tcgagttctc attgctgtcc gcgaacgaac tcgccgacta
      841 cgtgcggtgg atcggcaccg atgcgggcct cgacatcgac gagaccgcgg tcgaacacgt
      901 ggttcgccat ggtcgcggat cggcccgcga cacgttgtcg gcactcgaca tggtggtcgc
      961 cgccggcggg gtgagcgacc gggccgactc ggtcgatgag atcgtcgccg cgctgagcgg
     1021 gcgagatacc ggccgcgtga tgtcggcgat cgcggacgct gtggcacagg gccgcgagcc
     1081 gcgggtgctc gcggagaacc ttctgaccac gctacgcgac gcgtttctca catcggtcca
     1141 cgcgaccctc gatcatctct cggacgcgga tcagaccaag atggcagaga cggcagcagc
     1201 gcttggcacg gcgaccatca ccagggcgct tgaatccatc ggtgcggcga tcgtcgagat
     1261 gcgtcaggcg gcggatcccc gtgtgcccct cgaggtggcg ctgatcagac tgacgacacc
     1321 gagtgcatct gcggacctcg acgacctgct gcaacgcatc gaggacctcg agcgccgagt
     1381 tgcccgaggc gcaggctcgg caggtccggc cgccatcgac gctgacgcat cggatcagcg
     1441 agcgaacacg ccaatcgact cgccgaacga cgcaccccgt ggagcgccct ccgctccggc
     1501 tgccccgcag aggcccgtcg ctccgggagc ggcgtcgagc cctgccgaca tccgggcgca
     1561 gcttgcggcc cgcaagaatc ccggcggtcc cacttcagcg ccgccgattc cgtcacgtcg
     1621 cgccaccggt gccgccggtg ccgccggtgc cgccggggac gaccctgcca cacgtcggcc
     1681 acccgagccg ccccaggccg cggccgccgt atccgccccg cccaccgtcg cagcggagga
     1741 atcggaccga cctcaaacag tcgccggttc cggggccccg gtcacgctcg agcagctcgc
     1801 ggcggtgatg gccgagtcgg tcatcccaac cttgggcggg atgaacaagt cgctgttcaa
     1861 cgccgggcgc ttcgtcggtg agcgcgatgg catgtgggag ttcgccctcg acaacgctcc
     1921 ggaacgcgtg gtgagccggg cccagggggc gatcggcgat atcgaggcca tccttgcgtc
     1981 tcggctgggc gtggcggtgc gggtgcatct gacgagcacc acggcgggtg cttcaccaca
     2041 ggccattcga cgtgacgtcg gaccggcttc gacgccggcc gcgccggcga gccagtcgag
     2101 cggtgaacgc agcgcggctg gcccgcccgc caccaccgca gcggtctcgc ccgaggacgc
     2161 ggcaccgcac gatgaagatg acgacgaata tgtcgacatc gatgtccatg aactcgaaga
     2221 cgcaaacgac acggcaatct ccggcctcga caaggtcata gcggcctttc ccggcgcggt
     2281 ggtgatggag gaggaccaat gaccgacgat tcgaacaacc cgctcggcgg gctcgacctt
     2341 ggcgcgttgc tcggtgccgc tcagaacatg caggcacaga tgcacgagag ccagcagcga
     2401 ctcgccgaga ccgtggtcga aggcagcgcg gcgggtggac tcgtcaaggt gggggtcacc
     2461 gcagcg
//
LOCUS       DAOOMA010000432         2466 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_52613_length_2466_cov_3.425135, whole genome shotgun sequence.
ACCESSION   DAOOMA010000432 DAOOMA010000000
VERSION     DAOOMA010000432.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 2466)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2466)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2466
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52613_length_2466_cov_3.425135"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..787
                     /locus_tag="PLP95_10755"
     CDS             <1..787
                     /locus_tag="PLP95_10755"
                     /inference="COORDINATES: protein motif:HMM:NF028211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase C-terminal
                     domain-containing protein"
                     /protein_id="HPB46323.1"
                     /translation="TDYDGPLDDPQCTPDDIAYLLSAINGSNVNTVTTADIVGSWAGL
                     RPLVKSASSGRTADLSRGHNVHLAESGVVTITGGKLTTYRHMADDTVDVVVRDVLGAR
                     APEGARKCRTKKIRLRGADGFESVSEAAASYGVADHVAHHLSRRYGGEARAVMGLIKA
                     EPELGEPLVGGLPYVRAEAVFAVRHEMARTVDDVLSRRTRARLLARDDSAAAADDVAE
                     LIAADLGWDAEAAAASAAEFRAGVAHERDAAGLPEVLLAHALG"
     gene            858..1289
                     /locus_tag="PLP95_10760"
     CDS             858..1289
                     /locus_tag="PLP95_10760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB46324.1"
                     /translation="MTDTGLSISMIRSLDWATIWRAAVSTATFAVPLTLLQWWLIETD
                     RASKGDSLMLLLNLAIMGSGALGGFAAAKLAPRLALQHGAVAPALAAIAIQIVGAIRR
                     SIVGEEVSNVLGWIMLALLMAVFGMFGAWVNEKVSPPRTEG"
     gene            1291..>2466
                     /locus_tag="PLP95_10765"
     CDS             1291..>2466
                     /locus_tag="PLP95_10765"
                     /inference="COORDINATES: protein motif:HMM:NF012589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY family carbohydrate kinase"
                     /protein_id="HPB46325.1"
                     /translation="MTAILVLDIGTSGVRSAVVDETATIVVERFAEFLPDTPFDGIVE
                     YDAVTYNALAISVARDVLEDYRQTPGVTDPEIDAIGISNQRASTVVWDRHTGVPVAPA
                     QSWQDLRTIGDCLALQAAGHWTAPNQSITKAANIWNSVDPERERDLCIGTPDSWLVWS
                     LTEGAAHITEPTNVALTGFCELSGVDWNPELLALVDISESALPRIVDSSGELAWATVF
                     GPGRDGRGIPICGIAGDQQASLIGQGGLELGVAKLTFGTGAMLDISLGSQRPLEAIRS
                     EGGTFPIICWQLDGEQTWGLEAVMLSCGTNVQWLRDDLRLIETSADSETVAAQCESTD
                     GVVYVPAQLGLATPHWDYGARGTLLGLTRGTGRPQVVRAVLEGIAHRALDLIEAAEDD
                     "
BASE COUNT          391 a          862 c          832 g          381 t
ORIGIN      
        1 caccgattac gacgggccac tcgacgatcc gcagtgcacc cccgacgaca tcgcgtatct
       61 gctgtcggcc atcaacggca gcaacgtcaa cacggtcacc accgccgaca tcgtcggttc
      121 gtgggccgga cttcgcccgc tcgtgaagtc cgcatcgtcg gggcgcaccg ccgacctcag
      181 ccgaggccac aacgtgcacc tcgccgagtc gggggtcgtg acgatcaccg gcggcaaact
      241 caccacctac cgccacatgg ccgacgacac ggtcgatgtc gtcgtgcgcg acgtgctcgg
      301 cgcccgcgcc cccgagggcg cacggaagtg ccggaccaag aagatccgcc tgcgcggggc
      361 cgacggcttc gagtcggtga gcgaagccgc cgcgagttac ggcgtcgccg accacgtcgc
      421 acaccacctg tcgcgccgct acggcgggga ggcccgggcg gtgatggggc tcatcaaggc
      481 cgaacccgaa ctcggcgagc cgctcgtggg cggcttgccc tacgtgcgtg ccgaggccgt
      541 tttcgcggtc cgccacgaga tggctcgcac ggtcgacgac gtgttgagcc gccgcacccg
      601 cgcccgactg ttggcacgag acgacagcgc cgccgccgcc gacgacgtgg ccgaactcat
      661 cgcagcggat ctcgggtggg acgccgaggc tgcggcggct tccgcggccg agttccgcgc
      721 cggggtggca catgagcgcg acgccgccgg cctccccgag gtgttgttgg cccacgccct
      781 cggctgacgg gcagactggg agccatacat cgcccgtaca cgtcccccgt ccgggcctgc
      841 aacgaggatc cttcgccgtg accgacaccg gcctgtccat ctcgatgatc cgttcgctcg
      901 actgggcgac gatctggcgg gcagcggttt cgaccgccac cttcgccgtg ccgctcacgc
      961 tgttgcagtg gtggctcatc gaaacggatc gggcatccaa gggcgattcg ctcatgttgt
     1021 tgctcaacct ggcgatcatg ggcagcggcg ccctcggcgg gttcgccgca gccaagctcg
     1081 cgccgcgact tgcgctgcaa cacggcgcgg tggctccggc cctcgccgcc atcgccattc
     1141 agatcgtggg ggcgattcga cggtccatcg tcggcgagga ggtctccaac gtgctgggat
     1201 ggatcatgtt ggcgctgctc atggcggtgt tcggcatgtt cggtgcctgg gtgaacgaga
     1261 aggtctcgcc gccgagaacg gaggggtgac atgacagcga tcctggtgct cgacatcggc
     1321 acgagcgggg tgcgctccgc agtcgtcgat gaaaccgcga ccatcgtcgt cgaacggttc
     1381 gcggagttcc tccccgacac gcccttcgac gggatcgtcg aatacgacgc cgtcacctac
     1441 aacgcgttgg cgatctctgt ggcccgcgat gtcctggagg attaccggca aacacccggg
     1501 gtcaccgacc cggagatcga cgcgatcggc atttcgaacc agcgggcctc caccgtcgtg
     1561 tgggaccgcc acaccggggt gcccgtcgcg ccggcgcaga gctggcagga ccttcgcacg
     1621 atcggcgact gcctcgcact gcaggcggcc gggcactgga ccgcgccgaa tcagtcgatc
     1681 accaaggccg cgaacatctg gaactcggtc gatcccgaac gcgagcgcga cctgtgcatc
     1741 ggcactcccg actcgtggct cgtgtggtcg ctcaccgagg gggcggccca catcaccgaa
     1801 ccgacgaacg tggcgctcac cgggttctgc gagctgagcg gggtcgattg gaacccggaa
     1861 ctgctcgcac tggtcgacat ctcggagtcc gcgctccccc ggatcgtcga ttcctccggc
     1921 gagttggcat gggccacggt gttcggcccg ggccgcgacg gccgaggcat cccgatctgc
     1981 ggcatcgccg gcgaccagca ggcgagcctc atcggacagg gcgggctcga actcggggtc
     2041 gcgaagctca ccttcggcac gggcgccatg ttggacatca gcctcggctc gcagcgcccg
     2101 ctcgaagcca tccgttccga gggcggcacc ttcccgatca tctgctggca gctcgacggc
     2161 gagcagacct ggggccttga ggctgtgatg ttgagctgcg gtaccaacgt ccaatggttg
     2221 cgcgacgatc tccgcctcat cgagacctct gccgacagcg agacggtcgc ggcgcagtgt
     2281 gagagcactg acggcgtcgt ctatgtgcca gcccagctcg gtctggcgac accgcactgg
     2341 gactacgggg cacggggcac gttgctcgga ctgacccgcg gaaccgggcg accacaggtg
     2401 gtgcgcgcgg tgctcgaagg tatcgcccat cgagccctcg acctcatcga ggccgccgag
     2461 gacgac
//
LOCUS       DAOOMA010000433         2456 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_52931_length_2456_cov_3.742607, whole genome shotgun sequence.
ACCESSION   DAOOMA010000433 DAOOMA010000000
VERSION     DAOOMA010000433.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 2456)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2456)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2456
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52931_length_2456_cov_3.742607"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..823)
                     /locus_tag="PLP95_10770"
     CDS             complement(<1..823)
                     /locus_tag="PLP95_10770"
                     /inference="COORDINATES: protein motif:HMM:NF014998.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004144 - diacylglycerol O-acyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0045017 - glycerolipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="wax ester/triacylglycerol synthase family
                     O-acyltransferase"
                     /protein_id="HPB46326.1"
                     /translation="MERLSASDAAFLYMENRVVHMHVTGVLVLDPSTVPGGFTFDGFR
                     THVLNRLQQIPVFHQRLLRSPFGLDHPVWADDPDFNPTDHMRQVDLGGTATTSDLQAW
                     VGEFATARLDYSKPLWEMVVVEGFADGSVALVTKIHHVAVDGASGTDIMAQIVDLEAL
                     PPDTEELDLDAPTRLPNQAQLFVGAVASRVTSPLRGVRAFGRSLSSIGNVVREVTARG
                     DDRHSMARPFDAPRTFFNRSLSVRRSVSFGAAPLDDLKFVKSTFETTVNDVVLAAC"
     gene            complement(903..1295)
                     /locus_tag="PLP95_10775"
     CDS             complement(903..1295)
                     /locus_tag="PLP95_10775"
                     /inference="COORDINATES: protein motif:HMM:NF017426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF779 domain-containing protein"
                     /protein_id="HPB46327.1"
                     /translation="MSTARVVATGEALELIGRLTAINGPLMFHQSGGCCDGSSPMCFT
                     LGEFRVGDSDVLLGEVGGAPFYMSAEQFEYWKHTHLTLDVVAGRGGGFSLEAPEGVRF
                     LIRSRLFTDAEEADLAAVSTGASTPDSG"
     gene            complement(1298..>2456)
                     /locus_tag="PLP95_10780"
     CDS             complement(1298..>2456)
                     /locus_tag="PLP95_10780"
                     /inference="COORDINATES: protein motif:HMM:NF012398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HPB46328.1"
                     /translation="AIDHFRYFAGVLRAEEGGISQLDDTTVAYHFKEPLGVVAQIIPW
                     NFPILMATWKLAPALAAGNCVVLKPAEQTPMSILHLVELIGDLLPPGVLNVVNGFGVE
                     AGKPLASSPRVAKVAFTGETTTGRLIMQYASENLNPVTLELGGKSPNIFMADVMNADD
                     AFFDKALEGFTMFALNQGEVCTCPSRALIQESIYDAFMERALERVKAIVAGDPLDPAT
                     MIGAQASNDQLEKILSYLDIGRAEGAEVLIGGHQREVAGHEGGYYVEPTIFKGHNGMR
                     IFQEEIFGPVLAVTTFSDEAEALSIANDTLYGLGAGVWTRDMNTAYRLGRGIQAGRVW
                     TNCYHLYPAHAAFGGYKSSGIGRENHRMMLDHYQQTKNLLVSYSPDKLGFF"
BASE COUNT          434 a          770 c          815 g          437 t
ORIGIN      
        1 tgcacgcagc gagcaccaca tcgttcacgg tggtctcgaa cgtcgacttc acgaacttga
       61 ggtcgtcgag gggcgccgca ccgaaggaga cggatcgccg aacgctcaac gagcggttga
      121 agaaggttcg tggagcgtcg aacggacggg ccatggaatg gcggtcgtct ccgcgtgcgg
      181 tgacctcgcg cacgacgttt ccgatggaac tcagcgatcg tccgaacgct cggaccccgc
      241 ggagcggact cgtcaccctc gacgccaccg cgccgacgaa cagctgcgcc tggttgggca
      301 ggcgtgtagg tgcgtcgaga tcgagttcct ccgtgtcggg tggcagcgct tcgagatcga
      361 cgatctgggc catgatgtcg gttcccgaag cgccgtcgac ggcgacgtgg tgaatcttgg
      421 tgacgagcgc gaccgagccg tcggcgaacc cttcgaccac gaccatttcc cacagcggct
      481 tggagtaatc gagtcgcgcc gttgcgaact cgccgaccca cgcctggagg tcgctggtgg
      541 tcgccgttcc cccgaggtcg acctgtcgca tgtggtcggt gggattgaag tcgggatcgt
      601 cggcccacac cggatggtcg agcccgaacg gtgacctgag cagacgttgg tgaaagaccg
      661 gtatctgctg gagccggttg aggacgtggg ttcggaatcc gtcgaaggtg aatccgccag
      721 gaaccgtcga cgggtcgagc accaagacgc ccgtgacgtg catgtgcacg acgcggttct
      781 ccatatacaa gaaggcagcg tcagaggccg agagtcgctc cacgtccaca gtgtgtcact
      841 cgcactcgaa cgacaccgac cgattcgacc tattcggctg gagacgtgcc tcggggacag
      901 cgtcagccgg aatccggcgt cgacgctccg gtcgataccg ctgccaggtc ggcctcctcg
      961 gcgtcggtga acagccgtga cctgatcagg aatcgcactc cttcgggcgc ctcgagcgag
     1021 aagccgccgc cgcggccggc caccacgtcg agggtgaggt gggtgtgttt ccagtattcg
     1081 aactgctcgg cgctcatgta gaacggcgcg ccgcccacct cgcccaacaa cacgtcggaa
     1141 tcgccgacgc ggaactcgcc gagggtgaaa cacatcgggc tgctgccgtc gcagcatccg
     1201 cccgactggt gaaacatgag cggtccgttg atggcggtga ggcgaccgat gagttccagt
     1261 gcctcaccgg tcgccacaac ccttgccgtg gacatggtca gaagaacccg agcttgtcgg
     1321 ggctgtagct gaccaggagg ttcttggtct gctggtagtg gtcgagcatc atccggtggt
     1381 tctcccggcc gattcccgac gacttgtagc caccgaacgc agcatgagcc ggatacaggt
     1441 gatagcagtt cgtccacacg cggcccgctt gaatcccgcg gccgagccgg taggcggtgt
     1501 tcatatcgcg ggtccacacg ccggcaccga ggccatagag cgtgtcgttc gcgatgctca
     1561 acgcctcggc ctcatcgctg aaggtggtca ccgcgagcac cggaccgaag atctcctcct
     1621 ggaagatgcg catcccgttg tgacccttga agatggtggg ttcgacgtag tagccgccct
     1681 cgtggccagc gacctcgcgc tggtggcctc cgatgaggac ctcggcgcct tcggcgcggc
     1741 cgatgtcgag gtagctcagg atcttttcga gctgatcatt gctcgcctgc gctccgatca
     1801 tcgtcgccgg gtcgagcgga tcaccagcga cgatcgcctt cacccgctcg agtgcccgct
     1861 ccatgaaggc gtcgtagatc gactcctgaa tgagcgctcg cgacgggcag gtgcacacct
     1921 cgccctggtt gagggcgaac atcgtgaagc cctcgagggc cttgtcgaag aacgcgtcgt
     1981 ccgcgttcat cacgtcggcc atgaagatgt tgggcgactt gccgccgagt tcgagggtca
     2041 ccgggttcag gttctccgac gcgtactgca tgatgagccg tccggtcgtc gtttcgccgg
     2101 tgaatgcgac cttcgccacc cgcgggctcg acgccagtgg cttgccggcc tcgacgccga
     2161 agccgttgac gacgttgagg acgccggggg gcaacagatc gccgatcaac tcgacgagat
     2221 gcaggatcga catcggcgtc tgctcggccg gcttcagcac cacacaattt ccggctgcga
     2281 gcgccggcgc cagcttccag gtggccatga ggatcgggaa gttccacggg atgatctgtg
     2341 cgaccacgcc gagcggttcc ttgaagtggt aggcgaccgt ggtgtcgtcg agttgggaga
     2401 tgccgccctc ctcggcgcgc agcaccccgg cgaaatagcg gaaatggtcg atggcg
//
LOCUS       DAOOMA010000434         2445 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_53289_length_2445_cov_3.151464, whole genome shotgun sequence.
ACCESSION   DAOOMA010000434 DAOOMA010000000
VERSION     DAOOMA010000434.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 2445)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2445)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2445
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53289_length_2445_cov_3.151464"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..979)
                     /locus_tag="PLP95_10785"
     CDS             complement(<1..979)
                     /locus_tag="PLP95_10785"
                     /inference="COORDINATES: protein motif:HMM:TIGR00350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HPB46329.1"
                     /translation="MTHDDEQRSGDADEIWQRLRALEDTPLEDTPLERGAVPPSDSTR
                     MMPAPPPRTRGDGTGVDDLAGASGGDGAPGHYYVPAPQHRPQRSGAPAQPNPAAGRAA
                     YEQQQRAAVASNPAGNAGRQGTPTGSAAKAGSARAPQRSGSGGGRLRRWFRPKLRWIV
                     LYLPLLIMASIAALGLWGWKTVKDLGRVDVGDSIVAATGDTVNYLIVGSDSREGAEAE
                     GDAGYIGDTSVVGGQRADTIIVLRVEPTRSLMMSIPRDLWATNVATGKKGRINGTFNQ
                     GPDNLVRSVTANLGIPINQYLEVDFVSFAAMVDAMGGVDVEFPHAVFDKG"
     gene            1207..2070
                     /locus_tag="PLP95_10790"
     CDS             1207..2070
                     /locus_tag="PLP95_10790"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012478.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HPB46330.1"
                     /translation="MRRRRRGLAAPIIVVALTLLSACGSDDASVTTVTDGWDSPTAGV
                     AAGAPSITAPDGDPPTELQITDLETGDGTAAATGDLVVVNYIGALFDNGVVFDSSFDR
                     APFSVTLGQGNVIAGWDEGIVGMKPGGTRQLIIPPDMAYGSQARGNIPADSTLIFNVT
                     LNTVLTRPTDIAPVEGGTVDELRTTDLVVGDGEEATATSEVSLHYEAVHGSDATTFDS
                     SFERGQSFDVTLGQHQVIEGFEQGIVGMRVGGRRRIEIPAALGYGDTGAGTAVAPGET
                     LVFIVDLVAVN"
     gene            2071..>2445
                     /locus_tag="PLP95_10795"
     CDS             2071..>2445
                     /locus_tag="PLP95_10795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB46331.1"
                     /translation="MERHPTLVPTFRAVARSIVDDPAAEEIALAIDHQLYRESERFSD
                     HVLAWGVLLCAQSALAQASWGTPGPLTKERVEAMLALPAAALTVVVLRDGAGFGEDAI
                     AVAANRPVSEVRWLGDLLAPPTP"
BASE COUNT          389 a          849 c          796 g          411 t
ORIGIN      
        1 accccttgtc gaacacggcg tgcggaaact cgacatcgac gccgcccatc gcatcgacca
       61 tcgccgcgaa gctcacaaag tcgacctcga ggtactgatt gatcggaatc ccgaggttcg
      121 ccgtcaccga acgcaccagg ttgtcggggc cctggttgaa ggttccgttg atgcgcccct
      181 tcttaccggt ggcgacgttg gttgcccaca ggtcgcgggg aatcgacatc atcaacgaac
      241 gggtcggttc gacccgcaac acgatgatcg tgtccgcgcg ttggccgccg accaccgacg
      301 tgtcgccgat gtagccggca tctccttccg cctcggctcc ctcgcgggag tccgaaccga
      361 cgatcaggta gttcaccgtg tcgccggtgg ccgcaacgat ggagtcgccg acgtcgacgc
      421 gtcccagatc cttgaccgtc ttccaccccc acagccccaa tgccgcgatc gaggccatga
      481 tgagcagtgg caggtacaac acgatccatc gcagcttcgg tcggaaccat cgacgaaggc
      541 gcccaccgcc agaccccgag cgttgaggag cgcgagccga accggccttc gctgcagagc
      601 ccgtcggtgt gccttgccga ccggcgttac ccgcggggtt cgacgccaca gcggcacgct
      661 gctgttgttc ataggccgcc cgcccggcgg ccgggttggg ttgggccgga gcgccggacc
      721 gttgagggcg gtgctggggc gctgggacgt aatagtgccc cggtgcgccg tcgccgccgg
      781 acgccccggc gaggtcgtcg acacctgtgc cgtcgccgcg agtgcgaggc gggggcgccg
      841 gcatcatccg cgtcgagtcc gacggcggca ccgcgccccg ttcgagcggg gtgtcctcaa
      901 gtggcgtgtc ctcgagcgcc cgaagtcgtt gccagatttc gtcggcgtcg cccgagcgct
      961 gctcatcgtc gtgggtcacc gcaccatcat gtccgacacc atcggtcgtc ccggtggact
     1021 tcctgagtgc ccgccaattt caggcctgcg cctcacgtag cgcaaccacg tggaggcggc
     1081 aaatgggccg gtaggctagg tgaagtgaca tcgcgtcccc ccctcccccc gcacgatgaa
     1141 cctgccgcgt cgacgaggtc ttcgtcgtcg gcgttgccgt caaccaccag gtcgtcaaac
     1201 gtgccgatgc ggcgacggcg ccgcggcttg gcggccccga tcatcgtcgt cgcactgaca
     1261 ttgctgtccg cctgtggttc tgatgatgcg tcggtcacca ccgtcaccga cggttgggac
     1321 tcgcccaccg ccggggtcgc cgccggagca ccgtcgatca ccgctcccga tggcgacccg
     1381 ccgaccgaac ttcaaatcac cgatcttgaa acgggcgacg gaaccgcagc ggccaccggg
     1441 gatctcgtcg tcgtcaacta catcggcgcg ctgttcgaca acggcgtggt cttcgactcc
     1501 tcgttcgacc gggcgccctt cagcgtgacc ctcgggcaag gaaacgtcat cgccggatgg
     1561 gatgaaggca tcgtcgggat gaaaccgggt ggcacccgac agctcatcat cccgcccgac
     1621 atggcctatg gctcccaggc ccgtggaaac atcccggccg actcgacgct gatcttcaac
     1681 gtgacgttga acaccgtgct gacccgcccg accgacatcg ccccggtcga gggcggcacg
     1741 gtcgacgagt tgcgcaccac ggatctggtg gtcggcgacg gcgaggaagc gacggcgacg
     1801 agcgaggtct cgcttcacta cgaagccgtg cacggttccg acgcaaccac cttcgactcc
     1861 tcgttcgagc gcggccagtc gttcgatgtg accttggggc agcatcaagt catcgagggt
     1921 ttcgagcagg gaatcgtcgg catgcgtgtc ggggggcgcc gccgcatcga gattccggcc
     1981 gccctcggct atggagacac cggcgccggc accgccgtcg cccccggcga gaccctcgtg
     2041 ttcatcgtcg atcttgtcgc ggtgaactag atggaacgcc acccgacact ggtcccgacg
     2101 tttcgagcgg tcgcccgaag catcgtcgat gatccggccg ccgaggagat cgcgctcgcc
     2161 atcgaccacc agctgtaccg ggaatcggaa cgcttcagcg accacgtttt ggcctggggt
     2221 gtgttgttgt gcgctcagtc cgccctcgct caggcgagct ggggaacccc tggaccgctg
     2281 acgaaggaac gggtcgaggc catgttggcg ttgcccgccg cagcgctcac cgtggtggtg
     2341 ttacgcgatg gtgccgggtt cggggaggac gccatcgcgg tcgccgccaa ccggccggtg
     2401 tccgaggtcc gatggctcgg cgacctcctg gcgccgccca cgccg
//
LOCUS       DAOOMA010000435         2442 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_53372_length_2442_cov_4.215752, whole genome shotgun sequence.
ACCESSION   DAOOMA010000435 DAOOMA010000000
VERSION     DAOOMA010000435.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 2442)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2442)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2442
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53372_length_2442_cov_4.215752"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..695)
                     /locus_tag="PLP95_10800"
     CDS             complement(<1..695)
                     /locus_tag="PLP95_10800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB46332.1"
                     /translation="MTADTPSTTGSKAPRPDGTPRAETARAETARLAAWAMVALVVAL
                     FRNRLWATPNLAIFSELSKHLGSNPFEGSGIGGDYLLTNVLGQAIARLLGSTDPHLYV
                     RVHLGLFLVATALVVALTHRRFGYLSARTLVVALAAAPGTTVVMQWLGQPDALTFPLA
                     MSIVIARSWPARVALGVLLGLSHSEQGFAAALIAVVVSLAIDLLGDAPTAEGVYKLAS
                     RVPLTWITRPRLAA"
     gene            complement(718..1311)
                     /locus_tag="PLP95_10805"
     CDS             complement(718..1311)
                     /locus_tag="PLP95_10805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HhH-GPD-type base excision DNA repair protein"
                     /protein_id="HPB46333.1"
                     /translation="MSKVKLPITGNPDADQLLTDNPLALLLGMLLDQQVTMEKAFSGP
                     AVLAERLGHLDAARIAAMDLDEFIAVCAQTPAIHRYPTSMGTRVWEACVFISERYDND
                     ASKLWKRVRDGATLLTRVREVPGFGDEKSRIFVALLAKRFGRRPQGWELASEPFGDDQ
                     PRSVADVYDEASLLAVREAKKAAKAKKKAAAAAKVTA"
     gene            complement(1437..>2442)
                     /locus_tag="PLP95_10810"
     CDS             complement(1437..>2442)
                     /locus_tag="PLP95_10810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HPB46334.1"
                     /translation="ESVDGQILEEGITEAGSMASWTAAATSYATRGVPMLPFFIFYSM
                     FGFQRVGDLIWAAADARARGFLLGATAGRTTLAGEGLQHQDGHSLVLASTVPPCQAYD
                     PAFAYETAIIVREGIQRMYGDGEDVFYYLTLYNENYPMPAMPTHLTPEEVTSGIIEGL
                     YQWRPRPVGDLIPASILFSGTAWRAAADAAEILAEDYGVGADLWSVTSYKALREDAIA
                     VERHNRLHPGEPRRVAHVTEALQGDGPIVAVTDFMRMVPDQVAPYSPRPWTTLGTDGF
                     GRSDDRASLRRFFETDAAHVVVAVLSQLADLDTIDASVVRSALERFGVDPDVAPPWTR
                     "
BASE COUNT          391 a          767 c          900 g          384 t
ORIGIN      
        1 gcggccaggc gcggtctggt aatccacgtc agcggcaccc ggctcgcaag cttgtagacg
       61 ccctcggcgg tgggggcgtc gccgaggagg tcgatggcga ggctgacgac caccgcgatg
      121 agggctgccg cgaacccctg ctcggaatgc gacagcccca acagcacgcc gagcgccacg
      181 cgggcaggcc acgaccgggc gatgacgatc gacatggcga ggggaaaggt gagcgcgtcg
      241 ggttggccga gccattgcat caccaccgtg gtgcccggtg cggcggcgag ggcgacgacc
      301 agcgtcctgg cgctgagata gccgaaacgg cgatgggtca gtgccacgac caacgcggtc
      361 gcgacgagga acaggcccag gtggacccgg acgtacaggt gcgggtcggt cgagccgagg
      421 agtcgggcga tggcttgacc caacacgttg gtgaggaggt aatcgccgcc gatgccggac
      481 ccctcgaacg ggttcgatcc gaggtgcttc gacagctccg agaagatcgc caggttgggg
      541 gtggcccaga ggcggttgcg aaacagtgcc acgaccagcg cgaccattgc ccaggcggca
      601 agccgggcgg tttcggccct cgcggtttcg gccctcgggg tgccgtcggg cctcggcgct
      661 ttgctcccgg tggtcgaagg cgtgtcagcg gtcatgtcga aaaggcgatc tctcggttca
      721 cgccgtgacc ttggcggcgg ctgcggcctt cttcttcgcc tttgccgcct tcttcgcctc
      781 gcgcaccgcc agaagcgagg cttcgtcgta cacgtcggcg accgaccgcg gttggtcgtc
      841 gccgaacggt tcggaggcga gttcccaccc ctgcggtcgg cggccgaatc gctttgcgag
      901 caatgccacg aaaatccggg acttctcgtc gccgaacccg gggacctcgc ggacgcgagt
      961 cagcagggtg gccccgtcgc gcacccgctt ccacagcttg gaggcgtcgt tgtcgtagcg
     1021 ctcggagatg aacacgcacg cctcccacac ccgagtgccc atcgaggtgg gatagcggtg
     1081 tatcgccggg gtctgcgcgc acacggcgat gaactcgtcg aggtccatgg cggcgattcg
     1141 cgccgcgtcg aggtggccga ggcgttcggc gaggacggcg ggaccgctga acgccttttc
     1201 catcgtgacc tgctggtcca gcaacatccc gagcagcaac gccaacgggt tgtcggtgag
     1261 gagttggtcg gcgtcggggt tgccggtgat cgggagtttg accttggaca tggccccgat
     1321 tctggcacct cgacgtcgtc gatcggttcg acggggctga accgatcgac catgtcggac
     1381 tcggctggtg tcgaggtcga gcggtcgaaa cgagcggcga gcggttagcg gctaactcag
     1441 cgagtccacg gcggtgccac gtcggggtcg accccgaagc gttcgagtgc cgagcgcacc
     1501 accgacgcgt cgatcgtgtc gaggtccgcg agttgggaaa gcaccgcgac gaccacgtgt
     1561 gcggcatcgg tttcgaagaa tcgacgcagc gaggcccgat cgtcggagcg tccgaacccg
     1621 tcggttccga gcgtggtcca ggggcgaggc gagtaggggg ccacctggtc ggggaccatg
     1681 cgcatgaaat cggtgaccgc gacgatcggc ccgtcgcctt gcagcgcctc ggtgacgtgg
     1741 gccacgcgtc gcggctcgcc ggggtgaagg cggttgtggc gttcgacggc gatcgcgtcc
     1801 tcgcgcagtg ccttgtagga ggtgaccgac cagaggtcgg cgcccacccc gtagtcctcg
     1861 gcgaggatct ccgcggcatc ggccgccgcc cgccaggcgg tgccggagaa caggatggag
     1921 gccgggatca ggtcgccgac gggtcgcggc cgccactggt agagcccctc gatgattccc
     1981 gaggtcacct cctcgggggt gaggtgggtc ggcatggccg gcatggggta gttctcgttg
     2041 tagagggtga ggtagtaaaa gacgtcctcg ccgtcgccat acatgcgctg gatgccttcg
     2101 cgcacgatga tcgccgtctc atacgcgaag gccgggtcat aggcctggca cggcggaacg
     2161 gtggaagcga gcacgaggct gtggccgtcc tggtgctgga gcccctcgcc ggcgagcgtc
     2221 gttcggccgg cggtggcgcc gaggaggaag ccgcgcgctc gggcgtcggc ggccgcccag
     2281 atcaggtcgc caacccgttg gaagccgaac atcgaataga agatgaagaa cggcaacatg
     2341 ggcacgccgc gggtggcgta gctggttgcg gccgctgtcc acgacgccat cgaacccgct
     2401 tcggtgatgc cctcctcgag tatctgcccg tcgaccgact cg
//
LOCUS       DAOOMA010000436         2430 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_53739_length_2430_cov_3.839579, whole genome shotgun sequence.
ACCESSION   DAOOMA010000436 DAOOMA010000000
VERSION     DAOOMA010000436.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 2430)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2430)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2430
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53739_length_2430_cov_3.839579"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..488
                     /locus_tag="PLP95_10815"
     CDS             <1..488
                     /locus_tag="PLP95_10815"
                     /inference="COORDINATES: protein motif:HMM:NF016370.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF559 domain-containing protein"
                     /protein_id="HPB46335.1"
                     /translation="IEGQLVERRTTFDRLYATFERLAGQGRPGTRNMRAALGALDSKS
                     PTESELERRFSVLVDGLGLTQPNRQVTMPWMANERGRVDFVFVDERVIVELDGRRFHA
                     RDEAFDRDRRRDQEASMHGFETLRFTWRQVVDEPQRVAQVLSTVLRQRTATRTHQYTN
                     R"
     gene            513..1973
                     /locus_tag="PLP95_10820"
     CDS             513..1973
                     /locus_tag="PLP95_10820"
                     /inference="COORDINATES: protein motif:HMM:NF024571.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPB46336.1"
                     /translation="MTVPTFEFPMIDRFIDRVDELARLESWWNDDDALPINLFGRRRV
                     GKSWLFRRFAHGKPAILLVAHKVAPGAQLATFARALEPILGVVPELPDLASLFSTLYR
                     IARDERVLVVIDEFPWLLPSGEQATDRALTAVQAVIEHERDRSQLRLILCGSAIKQME
                     AMQTERNPLFGRFAPLHLRPLAFAEASLFLTELDPISRFERFAITGGMPRYLTTLGAD
                     RSLRSALAHQVLNHNAALWDEARTVLEQELGEPRLHFAILESLATGDKELNEIAQSTR
                     VPATSLSKYLNVLSDLGIVDRRLPIGARPNARGGHWHLLDPFFRFWFRFVFPYQDELE
                     NGLSGNDLFDAIISEQLHDHVAGEFERWCQRWTRTHRGTKSPTVGSWWGAATKHGHTS
                     AGRHTEEIDIVGIRQGRRVSIVGEAKWRTRKLDATILRDLYEYKLPALEAAGFTLDRD
                     LEVLLFARAGYSASLRAAADRDQRVTLVDVAAALAH"
     gene            complement(2126..>2430)
                     /locus_tag="PLP95_10825"
     CDS             complement(2126..>2430)
                     /locus_tag="PLP95_10825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB46337.1"
                     /translation="DDQRVGLVAHGHAEALTAADLTSGVVRIVNAAHEVFHHTPLDVR
                     VSLRGVDLGLVRWEPRLSTDGRLAGSLQIGRRALARVMRVGEALEVRVDNSVIVLE"
BASE COUNT          458 a          858 c          721 g          393 t
ORIGIN      
        1 tcatcgaggg gcaactcgtc gaacgacgca cgaccttcga tcggctctac gccacgttcg
       61 agcgacttgc cggccaagga cggcccggca cccggaacat gcgggcggcg ctgggggcct
      121 tggattccaa gtcgccgacg gaatccgaac tcgagcggcg cttcagcgtc ctggtcgacg
      181 ggctcggcct cactcaaccg aaccggcagg tgacgatgcc gtggatggcg aacgagcgcg
      241 gccgggtcga cttcgtgttc gtcgacgaac gggtcatcgt cgaactcgac ggccgccgct
      301 tccacgcccg cgacgaggcc ttcgaccgcg accgccgacg cgaccaggaa gcgtcgatgc
      361 acggcttcga gacgctgcgg ttcacgtggc gccaagtcgt cgacgagcct cagcgggtcg
      421 ctcaggtgtt gagcaccgtg ctgaggcaac gtacagccac tcgaacacat caatatacca
      481 accggtaatc taccatctcg tatattagat ctatgacggt cccgacgttc gaattcccga
      541 tgatcgaccg cttcatcgac cgcgtcgatg agcttgctcg cctcgagtca tggtggaacg
      601 acgacgacgc gttgccgatc aacctcttcg gccggcgccg tgtcggcaaa tcgtggcttt
      661 tccgacgatt cgcccacggc aagccagcga tcctgctcgt tgcgcacaag gtcgcccccg
      721 gagcgcagct cgcaaccttt gcccgagccc tcgaaccgat cctcggcgtg gtaccggagt
      781 tgcccgatct cgcgtcgctg ttctccactc tgtaccggat cgcacgcgac gaacgcgtcc
      841 tcgtcgtcat cgacgagttc ccgtggctcc tcccctcggg cgagcaggca accgaccgag
      901 cgctcaccgc ggttcaggcg gtcatcgaac acgaacgcga ccggtcacaa ctcaggctga
      961 ttctgtgcgg ctcggcgatc aaacagatgg aagcgatgca gacagaacgc aacccgctgt
     1021 tcggacgatt cgccccgctg cacctccgcc cgctcgcatt cgccgaggca tcgctgtttc
     1081 tcaccgaact cgaccccatc agccgattcg agcgattcgc catcaccggc gggatgcccc
     1141 gttacctcac cacgctcggc gcagatcgaa gcctgcgctc tgcactggcc caccaggtgc
     1201 tgaaccacaa cgctgcgctg tgggacgagg cacgaaccgt gctggaacag gaactgggag
     1261 aaccacggct gcacttcgcg atcctcgaat cgctcgcgac gggcgacaag gagctcaacg
     1321 aaatcgccca gtcgacgcgt gtgcccgcaa cctcgctctc gaagtacctc aacgtcctca
     1381 gcgacctcgg aatcgtcgac cgccgactcc ccatcggcgc ccggccgaac gcgcgcggtg
     1441 gacactggca cctcctcgat ccgttcttcc gcttctggtt tcgtttcgtc tttccgtacc
     1501 aggacgaact tgaaaacggc ctgagtggaa acgacctctt cgatgcgatc atttccgaac
     1561 agctccacga ccacgtcgcc ggcgagttcg aacgatggtg ccaacgatgg acccgcaccc
     1621 accgaggcac gaagtcaccc accgtcggtt catggtgggg agcggcaacg aagcacgggc
     1681 acacctccgc cggacgtcac accgaagaga tcgacatcgt cggaatccgc cagggcagac
     1741 gcgtcagcat cgtgggggaa gcgaagtggc gcacgaggaa actcgatgcg acgatcctgc
     1801 gcgacctgta cgagtacaag cttccagcac tcgaggccgc cggattcacc ctcgaccgcg
     1861 acctcgaagt cctgctgttc gcgcgcgccg ggtactccgc gtcactgcga gctgcggccg
     1921 accgagacca gcgcgtcacg ctcgtcgacg tcgccgccgc tctcgcgcac tgaaagtcgc
     1981 tcgcctgccg gccgtgcgcg aactttctgc cggaagtgct caaactttct gccggccgtg
     2041 cgcgaacttt ctgccgaaag tgacacgtga tgtcgcattt ggcagaaagt tcgcggcgag
     2101 gaggccgagc gggcggcgac cagcgctact cgagcacgat caccgagttg tcgacgcgca
     2161 cctcgagcgc ctcacccacc cgcatcaccc gggccagtgc gcggcggccg atctgcagcg
     2221 aaccggccag gcggccatcg gtcgacagcc ggggctccca acgcaccagg ccgagatcga
     2281 cgccgcgcaa cgacacccgc acatccaacg gcgtgtggtg aaacacctca tgggcggcgt
     2341 tgacgattcg cacgaccccg ctggtgagat cggccgcggt cagcgcctcg gcgtggccat
     2401 gcgccacgag acccacccgc tgatcgtcct
//
LOCUS       DAOOMA010000437         2429 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_53777_length_2429_cov_3.007582, whole genome shotgun sequence.
ACCESSION   DAOOMA010000437 DAOOMA010000000
VERSION     DAOOMA010000437.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 2429)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2429)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2429
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53777_length_2429_cov_3.007582"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..294)
                     /locus_tag="PLP95_10830"
     CDS             complement(<1..294)
                     /locus_tag="PLP95_10830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HPB46338.1"
                     /translation="MTDWNPVLRGEFDEGYWKELRAFVAAERSQTVVYPPQEQEFAAL
                     RLTPYAQTKVLILGQDPYHGPGQAHGLCFSVTDGVKPPPSLVNIYKELHSDLGC"
     gene            737..1228
                     /locus_tag="PLP95_10835"
     CDS             737..1228
                     /locus_tag="PLP95_10835"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HPB46339.1"
                     /translation="MAGVAGRDIYDRTADVAGWPQKFAALDAVLLEVLHRHEDRRHAV
                     HAAASDTWNILCATDGRASISAIASELGWSRRLITARFTEEYGLTPKAIAGVLRFHRS
                     VEWLRAHPTGSLAHAAAACGFSDHAHMTRQWRQICRTTPTGWLRDEVFPNVQDTAADD
                     MRR"
     gene            1233..1712
                     /locus_tag="PLP95_10840"
     CDS             1233..1712
                     /locus_tag="PLP95_10840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012225861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HPB46340.1"
                     /translation="MTEPRDPKSETHPNNAAPPTVWGSQNYTDANAAIEFLTNVFGFE
                     ATLVVEDPDDPQIIHHAELWWPEGGGFMLGTSQREGSEFSTLPGRVGMYVVTADPFPI
                     YDRCVAAGARIEREMESTPYCAAQFTVADPEGNMWCFGTYRGLSEPGDAGARVTFEE"
     gene            1775..2053
                     /locus_tag="PLP95_10845"
     CDS             1775..2053
                     /locus_tag="PLP95_10845"
                     /inference="COORDINATES: protein motif:HMM:NF025587.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate and iron-dependent oxygenase
                     domain-containing protein"
                     /protein_id="HPB46341.1"
                     /translation="MTYQPSPSIAVSRHGATAPTLVEVDLRRWDGTETQRRELAEEVR
                     EICHHVGFFELVGHGVPDEFRRRHFDFVERFFALPLEHRSAIDKARSP"
BASE COUNT          438 a          877 c          716 g          398 t
ORIGIN      
        1 gcagcccagg tcgctgtgga gctccttgta gatgttcacc aacgacggcg gtggcttcac
       61 gccgtcggtc accgagaagc acagcccgtg cgcctgcccc ggaccgtggt acgggtcctg
      121 gccgaggatc agcaccttcg tttgcgcgta cggcgtgagt cgcagcgccg cgaactcctg
      181 ttcctgtggc ggatagacga cggtttgcga gcgctcagcg gccacgaaag ctcgaagttc
      241 cttccagtac ccctcgtcga actcgccgcg cagcaccgga ttccagtcgg tcatcacggt
      301 gcccattctg cttcgactcg ttacgaggag cgacactcgg agatacggtg gcaacgatgc
      361 ccggatccgc tccgacgacg ctcaacgaga tgatccccca ccggccgcca ccggcgctgg
      421 cgggctactt cgactcgatc gtcgggtatc gcatgtccgg gttccccgcc ggggtccacc
      481 tcggcatgcc gtctcgttcc ctcaccgtca tcatcagcta cgacgatcca ctccggttgc
      541 ggatgcatcc cgacacggac catgaaggct cgacccatct ggcgatggcg agcggcatcc
      601 atccgatcgc cgtcgaaatc gatcacaccg ggaaccagca cggcatccaa ctgaacttca
      661 caccccaagg cgcccgggcg ttgttcgcca tgccctcgaa ggaactcgcc ggccttgcgg
      721 tcgacctcga cgacctgtgg ccggggtcgc tgggcgcgac atctacgacc gaaccgccga
      781 tgttgcgggt tggccgcaga aattcgccgc gctcgacgcg gtgctcctcg aggtgctgca
      841 tcgccacgag gaccgccgtc acgctgtcca cgccgcggcg agcgacacgt ggaacatctt
      901 gtgtgccacc gacggtcgcg cttcgatctc cgcgatcgcg agcgaactcg gctggagtcg
      961 acggctcatc accgcacgct tcaccgagga atacggcctc acccccaagg ccatcgccgg
     1021 agtcttgcgc tttcaccgct ccgtggaatg gctacgtgcc cacccgaccg gctccctcgc
     1081 ccatgcagcc gcggcgtgtg ggttttccga tcatgcccac atgaccaggc aatggcgcca
     1141 aatctgtcgc accacgccga cgggatggct gcgcgacgag gttttcccaa acgttcaaga
     1201 caccgccgcc gacgacatga gacgctgacc acatgaccga accacgcgac ccgaaaagcg
     1261 aaacccaccc gaacaacgca gccccgccga cggtgtgggg cagccagaac tacaccgacg
     1321 cgaacgccgc gatcgagttc ctcaccaacg tcttcggctt cgaggcgacg ctcgtcgtcg
     1381 aggatcccga tgaccctcag atcatccacc acgcagagct ctggtggccc gaaggcggag
     1441 gattcatgct cggcaccagc caacgcgagg gatcggagtt ctcgacgctg cccggccgtg
     1501 tgggcatgta cgtcgtcacc gcagatccgt tccccatcta cgaccgatgc gtcgccgccg
     1561 gagcccgaat cgaacgcgag atggagtcga ctccctactg tgcggcgcag ttcaccgtgg
     1621 ccgatcccga aggcaacatg tggtgctttg gaacctaccg aggcctcagc gaaccgggcg
     1681 acgcaggtgc ccgcgtcacc ttcgaggaat gaatcgatgc tgaatcaaag ctgacggttc
     1741 gcgacggttt cgacgagcgg cctacagtcg ctccgtgacc taccaaccgt cgccttcgat
     1801 cgctgtgtcc cgacacggcg cgaccgcccc gacgctcgtc gaggtcgacc ttcgccgctg
     1861 ggacggaacc gagacgcaac ggcgcgaact cgccgaggaa gtccgcgaga tctgccacca
     1921 cgtcgggttc ttcgaactcg tcggtcacgg cgttcccgat gagttccgtc gacgacattt
     1981 cgacttcgtc gaacggttct tcgcgttacc cctcgagcac cgaagcgcga tcgacaaggc
     2041 caggtcccct tagtttcggg gatgggagtg cgtcggtgcg gaactcaccg acaaccgggt
     2101 cgactaccgc gaacaactcg acctcgcggt ggaatacgag gcctaccccg tcggcgtcga
     2161 accggcctac ctgcgcagct accccgacat cgtcgccgta catcaccccg actggagctg
     2221 aatcgacgac gccgtcgggg cgccgcaccg tagggtggcc ccatgtgtga agcatgcggc
     2281 cccctcggag tcatcgctcg aacccgcacg gttgctcgcc cgacaggtcc catgcgcccg
     2341 gcccgcctcg cccggatggc caccgcgcac caccgggtca cccccggttc cgcccgcccg
     2401 atcgaacgac tcctcctcgg cgccaccgt
//
LOCUS       DAOOMA010000438         2427 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_53853_length_2427_cov_2.896712, whole genome shotgun sequence.
ACCESSION   DAOOMA010000438 DAOOMA010000000
VERSION     DAOOMA010000438.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 2427)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2427)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2427
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53853_length_2427_cov_2.896712"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(172..1113)
                     /locus_tag="PLP95_10850"
     CDS             complement(172..1113)
                     /locus_tag="PLP95_10850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020502092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HPB46342.1"
                     /translation="MRFGLIALDGCFGSAVASVIDIVRVADRVRGDVDPHIDPIDLAI
                     VGPKRRVTTTTSMTLSVEHPLSECGDFDVVVVPALGTLTAAATNDALQSRDARSVIES
                     LRRLDDATTQIAAACTGVFAVAETGRMHHRRATTSWFLGPEFRTRYPTVDLDLDTMVV
                     VDGNLLTAGAAFAHIDLALSLVRSISPDLAQHVAKLLLIDERPSQAAFVAYEHLHHDD
                     PIVVEFERLVRARLDEPLDIASVAQSLGTSRRTLERRVRSALNLTPLGFVQRLRIERA
                     RHLSATTDLNSAEIAMRVGYANAETLRSLLRRQRRRS"
     gene            1219..1530
                     /locus_tag="PLP95_10855"
     CDS             1219..1530
                     /locus_tag="PLP95_10855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009883809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HPB46343.1"
                     /translation="MSTPTSLPYAFVAKIVAADGQHDALGDLLASAVELANDEEGTIV
                     WFAVRTHPDTFWIFDAFPDESARDAHANGAIVEALMANQQLLGAAPEILPADVLASKL
                     P"
     gene            complement(1833..>2427)
                     /locus_tag="PLP95_10860"
     CDS             complement(1833..>2427)
                     /locus_tag="PLP95_10860"
                     /EC_number="2.1.1.100"
                     /EC_number="2.1.1.334"
                     /inference="COORDINATES: protein motif:HMM:NF018649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HPB46344.1"
                     /translation="PMRDRNHQPPMFRDRPTAAAAVATGALLTVLELTDVLAPTTPDD
                     ELHDQHTSAWFAATMATAIVSSTIALRASRPIPRRPRTWWAGLGLVWTGAIVNRLARR
                     QLGTNSRSRITVVPAHEIVRTGLYRHVRHPMYSGSTLICGGIATALSAPASALWALPI
                     VSLLHRIHIEEAKLQAALGSDYHRYRQGRARLIPGIW"
BASE COUNT          401 a          779 c          855 g          392 t
ORIGIN      
        1 cgttcgatca cctccgcgat cgctcgacga cgcagctccg gcgagtactt cgatggacgt
       61 gccatgacgg cagtctcctt ccccgcaacc cgaaggccac ggaatcgaga ctgcaccaaa
      121 cccgggggcc ttgagagttc tattacaccg gctacgcgcg tggcgctggg ctcacgatcg
      181 gcgtcgttgt ctgcgcagga gggagcggag tgtctcggcg tttgcgtagc cgacccgcat
      241 ggcgatctcg gcggagttga ggtcggtggt tgccgagaga tgccgagccc gttcgatgcg
      301 aagacgttgg acgaagccga gcggagtgag gttgagtgcc gagcggactc gtcgttcgag
      361 ggtgcgccgg ctggtgccga gcgactgcgc gacggacgcg atgtcgagcg gttcgtcgag
      421 gcgggcgcgc acgagacgtt cgaactcgac gacgatcggg tcatcgtggt ggaggtgttc
      481 gtaggcgacg aaggccgcct gcgacgggcg ctcgtcgatg agaagcagct tggctacgtg
      541 ctgggccagg tcggggctga tcgatcgcac gagtgagagt gcgaggtcga tgtgggcgaa
      601 ggcggcgccg gcggtgagaa ggtttccgtc gaccacgacc atggtgtcga gatcgaggtc
      661 gacggtcgga tagcgcgttc ggaactccgg ccccaggaac cagctggtcg tcgcccgccg
      721 gtggtgcatc cgtccggtct cggcgacggc gaacacgccg gtacacgccg cggcgatctg
      781 ggtagtcgcg tcgtcgagcc gcctgagtga ctcgatgacc gaacgagcat ctcggctctg
      841 cagcgcgtcg ttggtggccg cggcggtgag ggttcctagc gcggggacga cgacgacgtc
      901 gaagtcgccg cactcggaca gcgggtgctc caccgacagg gtcatcgatg tcgtcgtggt
      961 cacccgtcgc ttcggtccga cgatggcgag gtcgatcggg tcgatgtgtg ggtcgacatc
     1021 gccgcggact ctgtcagcca cccgcacgat gtcgatcacc gacgcgaccg ccgagccgaa
     1081 gcagccatcg agcgcgatca atccgaaacg catggcgtga acagtagcaa tgctgccgta
     1141 tacgccactc cccgttgccc ctggctcgtc gtacgttgga cttcccatac gaacaacccc
     1201 gtaagcaagg agaccaccgt gtccacaccc acatcactcc cgtacgcctt cgtcgccaag
     1261 atcgtcgcgg ccgacggaca gcacgacgcg ctcggcgatc tgctcgccag cgctgtcgaa
     1321 ctcgccaacg atgaagaagg aacgatcgtc tggttcgcgg tccgaaccca ccccgacacc
     1381 ttctggatct tcgacgcatt tcccgacgag tccgctcgcg acgcccacgc caacggcgcc
     1441 atcgtcgaag ccctgatggc aaaccagcag ctcctcggcg cagcgcccga gatcctgccg
     1501 gccgacgtcc tcgcgtccaa gctcccgtag tccaccaccg cacgagacga tcgcgccccg
     1561 tggagcggtc gggcccagtc gcctcgaccc cgcaccacgt tgacgatccc cgacgggccg
     1621 atcgcgagga ggcgaggcac caccgatcgc acgcccatat ctgcgtgggg cgccgtctcc
     1681 aaggtgccgg tcgatgacat gacgccaggc tgaccctgaa acgcatcccc gaccgcgccg
     1741 ttcggatcag ccgcgttgcc gccagtcata gctgtccgcc agcattcgga gcccgttccg
     1801 gccatgagcc tcgctgcgtc cgtagcggtg cgctaccaga tccccgggat cagacgagcc
     1861 cgcccctgcc ggtagcggtg gtagtcactc ccgagggcgg cctggagctt ggcttcttcg
     1921 atgtggatgc gatgaagcaa cgacacgatc ggcagggccc acagcgctga ggctggtgcg
     1981 ctcagggcgg tggcgatgcc gccgcagatg agggtcgagc cgctgtacat cgggtggcgg
     2041 acgtgtcggt agaggcccgt gcgcacgatc tcgtgggccg gcacgacggt gatgcgggac
     2101 cgggagttcg tgccgagctg tctgcgtgcg agacggttga cgatcgcgcc ggtccagacg
     2161 aggccgagcc cggcccacca ggtgcgcgga cgtcgcggga tcgggcggct ggccctgagt
     2221 gcgatggttg aactgacgat cgccgttgcc atggttgcgg cgaaccaggc gctggtgtgc
     2281 tggtcgtgca gctcgtcgtc gggggtggtg ggcgcgagaa cgtcggtgag ctcgagcacg
     2341 gtgaggagcg cgccggtcgc tacggcggca gcagcggtgg gccggtcgcg gaacatcgga
     2401 ggctggtgat tccggtcacg catcggc
//
LOCUS       DAOOMA010000439         2422 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_54011_length_2422_cov_3.665399, whole genome shotgun sequence.
ACCESSION   DAOOMA010000439 DAOOMA010000000
VERSION     DAOOMA010000439.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 2422)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2422)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2422
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54011_length_2422_cov_3.665399"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..320)
                     /locus_tag="PLP95_10865"
     CDS             complement(<1..320)
                     /locus_tag="PLP95_10865"
                     /inference="COORDINATES: protein motif:HMM:NF014085.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB46345.1"
                     /translation="MSPGILALVPIRSLDSGKTRLASSLDEDQRRTLTLALATSVLDV
                     LAATRSVDVAVVTSDDDVARWSRDQRTVALAPPSEGLNEAAAFGLNEAADRGYRHAAI
                     VHADL"
     gene            417..1439
                     /locus_tag="PLP95_10870"
     CDS             417..1439
                     /locus_tag="PLP95_10870"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HPB46346.1"
                     /translation="MRITVGVIGGGSWGTTVASLAAHNVSTILWGRDAATVAEINESH
                     HNSRYLAGLDLHPKLRATTDLREAVEQADVIVMGVPSQAMRSTLEQVAEHIRAWVPIV
                     SLAKGLEQGTRLRMSQVANEVLPGHPVAVLTGPNLAKEILTGDAAASVLATHDPVVAA
                     RLQEVFSTAMFRVYTNPDVIGCELAGALKNVIAIASGMADGLGAGDNTRAAVITRGLA
                     ELSRLGVAMGGNELTFAGLAGMGDLLATCVSPQSRNRFVGEQLGPGRTIEEIIDSMNM
                     VAEGVKTAPVVLDLGEEYGIDLPISTQVYEVCHEAKSASAAYAGLLRRGGLVEHPSND
                     FVFPKI"
     gene            1454..2146
                     /locus_tag="PLP95_10875"
     CDS             1454..2146
                     /locus_tag="PLP95_10875"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HPB46347.1"
                     /translation="MPKTPAPGLNFERNHWEAGVDTVVGLDEVGRGAWAGNLTIAAVI
                     VARDRRVNGIRDSKMLSEPRREVLYERIVSWCEGWAVGHASARECDELGMSAAQKLAA
                     RRALVALDRPVEVALVDGPWDFIEGGPDGLTRTETIVKGDRLSLSIAAASIVAKVTRD
                     RLMREAALHFPWYHFDTNKGYPCPRHKEALHGYGVSSIHRRSWAFVDGLAWTSQHRRS
                     VEAAEQYEFDLF"
BASE COUNT          405 a          745 c          873 g          399 t
ORIGIN      
        1 agatcggcgt gcacgatcgc ggcgtggcga tagccgcgat cggcggcctc gttgaggccg
       61 aacgcagcgg cttcgttgag cccctcgctc ggcggggcga gcgcaaccgt gcgctgatcc
      121 cgcgaccagc gggcgacgtc gtcgtcggag gtcacgaccg cgacgtcgac cgaacgggtc
      181 gctgcgagga cgtcgagcac cgaggtggcg agcgcgagcg tcagcgttcg acgttggtcc
      241 tcgtcgaggc tcgacgcgag tcgcgtcttg cccgagtcga gtgatcggat gggtacgaga
      301 gcgagaatgc cgggggacat cgtcacgagg ttagaacgaa accgccccgc cgtcgccagt
      361 cgaatgaccc gagcgcgtag ggtgtttggc caaacgacaa cgggggagac cagcccatgc
      421 gcatcacggt gggagtaatc ggcggtggtt cgtggggcac cacggtggcg agcctggccg
      481 cgcacaacgt gtcgacgatc ctgtgggggc gagatgccgc cacggtggca gaaatcaacg
      541 agtcgcatca caacagtcgg tatctcgccg gcctcgacct tcatccgaaa ctgcgggcga
      601 ccaccgacct gcgtgaggcg gtcgaacagg cggatgtgat cgtgatggga gtcccgtccc
      661 aggccatgcg ttcgacactc gaacaggtcg ctgaacacat ccgtgcctgg gtgccgatcg
      721 tgagtctggc caagggcctg gaacagggca cgcggcttcg gatgagccag gtcgccaacg
      781 aagtgctgcc cggtcacccc gtggcggtgc tcaccgggcc gaacctggcc aaggagatcc
      841 tcaccggaga cgccgcagcc tcggtgttgg ccacccacga tccggtcgtc gccgccaggc
      901 tccaggaagt gttcagcacg gcgatgtttc gggtgtacac caaccccgac gtcatcggtt
      961 gcgagctggc gggagcgttg aagaacgtca tcgccatcgc ctcgggcatg gccgacggcc
     1021 ttggcgccgg agacaacacc cgcgccgcgg tcattacccg gggacttgcc gaactcagcc
     1081 gcctcggggt ggcgatgggc ggcaacgagt tgacctttgc ggggctggcg gggatgggcg
     1141 accttttggc cacgtgcgtg tcgccgcaga gccgcaaccg cttcgtcggc gaacagttgg
     1201 gcccagggcg cacgatcgag gagatcatcg attcgatgaa catggtcgcc gagggggtca
     1261 agacggcgcc cgtggtgctc gatctcggtg aggagtacgg catcgatctg ccgatctcga
     1321 cccaggtgta cgaggtctgt cacgaggcga agtcggcctc ggccgcgtat gcggggctgt
     1381 tgcgtcgggg cggtctcgtc gagcacccgt cgaacgactt cgtgtttccg aagatctgaa
     1441 cggccggtcg tcggtgccca agacccccgc acccggcctc aactttgagc gcaaccattg
     1501 ggaggccggg gtcgacacgg tggtcggcct cgacgaggtc ggccgcggcg cgtgggctgg
     1561 gaacctgacg atcgcggcgg tgatcgtggc ccgcgaccgc cgggtgaatg gcatccgcga
     1621 ttccaaaatg ttgagcgagc cgcggcgtga ggtcctctat gagcgcatcg tttcgtggtg
     1681 cgagggctgg gcggtgggcc atgcgagcgc acgggaatgc gatgaactcg ggatgtcggc
     1741 ggcgcagaag ctcgcggcgc gccgtgcgct cgtcgctctg gaccgtccgg tcgaggtcgc
     1801 gctcgtggac gggccgtggg atttcatcga gggagggccc gacgggctga cccggaccga
     1861 aaccatcgtg aagggggacc ggttgtcgtt gtcgatcgcc gctgcctcga tcgtggcgaa
     1921 ggtcacccga gatcgactga tgcgcgaggc ggcgctgcat ttcccgtggt accacttcga
     1981 cacgaacaag ggctacccgt gcccgcgcca caaggaggcc ctgcacggct acggggtgtc
     2041 gtcgattcat cgacggtcgt gggccttcgt cgacggtctg gcgtggacgt ctcagcatcg
     2101 tcggtccgtc gaggcggccg agcagtacga gttcgatctg ttctgagcga cggccgcttg
     2161 agcgacggcc tcttgaacat cggtctgtcg ggtgcgcgcg caatcacacg ggtaagcaat
     2221 atgcttgcag gagtaagcag tccatgaaac gatcgaagtg tcatgtcgaa tcgaacccaa
     2281 cgcccctccg cccatgtgcg tcccgccgtt gccgccccct tcgacggggt gcagcgggcc
     2341 atgcgtcgcc tcacgcgggc tgacgcgtcg aatctggggc ccgatggatc gccgatcgac
     2401 gggtccaggg gctccacgga gg
//
LOCUS       DAOOMA010000440         2420 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_54074_length_2420_cov_3.747569, whole genome shotgun sequence.
ACCESSION   DAOOMA010000440 DAOOMA010000000
VERSION     DAOOMA010000440.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 2420)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2420)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2420
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54074_length_2420_cov_3.747569"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            155..1477
                     /locus_tag="PLP95_10880"
     CDS             155..1477
                     /locus_tag="PLP95_10880"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HPB46348.1"
                     /translation="MTNEAKASVSGAVELRTDRRFPKIAMLVARFPKVTETFQLREMV
                     TFEQLGVPIELYAITHHDDGGTIQEDARSLDARANYFPRISFEMIRAQLTWIRRNPSA
                     YLKAWKWSLKTFASAPEMFVRSFVVVPLAATMALRMERDGIEHIHAHFATYPTQAALI
                     IHWLTGLPYSFTGHAHDLQMRHEGLRDKITESEFFVTCTQYSQDSLRDLYGAIVLDKG
                     HVVHHGVNLDKFTYREPPAPTNEDGSPRPLRMVCTATFTEIKGHEYLIEAIRLLRDRG
                     KDIDLKLIGGDPPQQSNLQNEIRAQVRAAGLDERVEFLGKRPSDEVMRWIEWADVGVL
                     ASVRSDTGDMDGLPNVLTECLAMGRPVVSTRQPGTMELVEHGVNGLLARTRDAEELAA
                     CIEQMYDDPDARVRMGIAGHAKVVANENVVDRTTELLNIYAERVGRPL"
     gene            1481..2335
                     /locus_tag="PLP95_10885"
     CDS             1481..2335
                     /locus_tag="PLP95_10885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB46349.1"
                     /translation="MAPRRVSIRGSLRFSAESLGFALETFAGPVAAEASLRRLAVDPR
                     DRLTSFVESVGHRVRFDGSLVEIEPAAEDGTPPWSERADRLFATLVHCADGGLLNVES
                     AGESTVVSAGRAVAPLVAGDFEWIEPDPKLPPGSRSVAIPDWVRLSSAISERQFTLLS
                     PSPLAPALPPSFAMVDLGVTPASANSGASSFVGRIGDGEMLIAWRPLDDDRGHLTRAL
                     LERNVDHSVGGAQELLTAIAAARLYVGVILSDDPSGEDPRLGFMESVAVELGAVVMDG
                     VGRLVRQR"
BASE COUNT          413 a          732 c          829 g          446 t
ORIGIN      
        1 gccgagcgtg tgaagaaggt gaaggtgttc catgaagaat ggctgcctga caccgatctg
       61 atgaccccca cccagaagct caagcggcgc ggcctgcgag ccaagttcgc cgacgacatc
      121 gctgcgctgt actcctgagc gcaagggagc tcagatgacc aacgaggcca aggcgtcggt
      181 gtccggcgcg gtcgaactgc gaaccgaccg tcgattccca aagatcgcca tgctggttgc
      241 ccggtttccg aaagtgaccg agacgttcca gcttcgcgag atggtgacct tcgaacagct
      301 cggggtcccg atcgagctgt acgcgatcac acatcacgac gacggtggaa cgattcagga
      361 ggatgcccgc tcgctcgacg ctcgggcgaa ttacttcccg cggatcagtt tcgagatgat
      421 ccgtgcacag ctcacgtgga tccgccgtaa tccgagtgcc tacctcaagg cttggaagtg
      481 gtcgttgaag acgttcgcgt cggcaccgga gatgttcgtt cgatcgttcg tcgtggttcc
      541 gctcgccgca acgatggcgt tgcggatgga acgtgacggg atcgaacaca tccacgcgca
      601 cttcgcgacc tacccgaccc aggcagcgct catcatccat tggttgaccg gtctgccata
      661 ttcgttcacc ggccacgcac acgaccttca gatgcgacac gagggacttc gggacaagat
      721 caccgagagc gagttcttcg tgacgtgcac gcaatacagc caggattcgt tgcgcgatct
      781 gtacggagcg atcgttctcg acaagggcca tgtggttcat cacggggtga acctcgacaa
      841 gttcacctac cgcgagccgc cggcgccgac gaatgaagac ggctcgccgc gaccgctgcg
      901 gatggtgtgc accgccacgt tcaccgagat caagggccat gagtacctga tcgaggcgat
      961 tcggctgctg cgagaccgcg gcaaggacat cgacctgaag ctgatcggtg gcgatccgcc
     1021 gcagcagtcg aacctgcaga acgagattcg ggcacaggtg cgcgcagcgg gtctcgacga
     1081 acgcgtcgag tttctcggca aacgcccgtc cgatgaggtc atgcggtgga tcgaatgggc
     1141 cgacgtcggg gtgttggcct cggtgcgttc cgacaccggt gacatggacg gtttgccgaa
     1201 cgtgttgacc gaatgcctgg cgatgggtcg gccggtggtg tcgacgcgcc agccgggcac
     1261 gatggagctc gtcgaacacg gcgtgaatgg tctgctcgcc aggactcggg acgcggagga
     1321 actcgctgcg tgcatcgagc agatgtacga cgaccccgac gctcgtgtgc ggatggggat
     1381 cgcgggccac gccaaggtgg tcgcgaacga aaatgtcgtc gaccgcacca cggaactgct
     1441 gaacatctac gccgaacgcg tcggccgtcc tctgtagcgc atggccccga ggcgggtatc
     1501 gatccgcggc agccttcgat tctccgccga gtcgttgggc ttcgcactcg agacgttcgc
     1561 tgggccggtt gcggcagagg cctcgttgcg ccgcttggcg gttgaccccc gcgatcggct
     1621 gacgtcgttc gtcgagtcgg tcggccatcg cgttcgcttc gacggctcgc tcgtcgagat
     1681 cgaaccggcc gctgaggatg gcaccccacc gtggagcgag cgggcggatc ggctgttcgc
     1741 gacgctggtg cactgtgccg atgggggttt gctcaacgtc gagtcggctg gcgagtcgac
     1801 ggtggtgtcg gccggccgag cggtggctcc attggttgcg ggggacttcg agtggatcga
     1861 gccggacccg aaactgccac cagggtcgcg cagcgttgcg attcccgact gggtgcggct
     1921 gtcgtctgcg atttcggagc ggcagttcac gttgttgtcg ccgagcccgt tggccccggc
     1981 gctgcccccg tcgttcgcca tggtcgacct tggggtgacc ccggcgtcgg cgaacagcgg
     2041 tgcctcctcg ttcgtgggac gcatcgggga cggcgagatg ctgatcgcgt ggcgaccgct
     2101 ggacgatgat cgcggtcacc tcacccgcgc gttgctcgaa cgaaacgtcg atcactcggt
     2161 ggggggtgcg caggaactgc tcactgcgat cgccgccgca cggctctacg tcggggtgat
     2221 cctgtcggac gacccgagtg gcgaggaccc gcgactcgga ttcatggagt cggtcgcggt
     2281 ggagctcggt gcggtcgtga tggatggggt tggccggctg gtgcgacagc gctgacccat
     2341 cacgaaacgt gtcgtctgcg gccagcgtct ggctgaatcg tcgactattc gggcgtgtcc
     2401 gacgtgtccg acgtgtccgg
//
LOCUS       DAOOMA010000441         2407 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_54488_length_2407_cov_4.317177, whole genome shotgun sequence.
ACCESSION   DAOOMA010000441 DAOOMA010000000
VERSION     DAOOMA010000441.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 2407)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2407)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2407
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54488_length_2407_cov_4.317177"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..439)
                     /locus_tag="PLP95_10890"
     CDS             complement(<1..439)
                     /locus_tag="PLP95_10890"
                     /inference="COORDINATES: protein motif:HMM:NF013884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HPB46350.1"
                     /translation="MTVDRTAGSERSFGLDIARVLAAIAVLISHVAFVTGVVNPERWD
                     SWLRLILPRLDVGVPVFFVLSGLLVGRPFVRSLVRNTDPPDLATFTLRRFARIFPLYW
                     VVLAATLAFGAGAAPSPQRLVATMALVHIYVPGWAIGPITQSWT"
     gene            470..>2407
                     /locus_tag="PLP95_10895"
     CDS             470..>2407
                     /locus_tag="PLP95_10895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB46351.1"
                     /translation="MADADRPHPTSPPDEPVERSDRSLALDAVDGPGRARRGTLDPVR
                     WTMVVLIALVVGLGAAASIRGWIPVGDNAFLSYRSRHVGSSDPILLNNASSGGTDNSE
                     PYNHPGAFPLTLMAPITLIGGPGSVPLAAAVCTAASIGLISSTVRRVAGRGAQLWSLV
                     VSAGLLATQSVTMFADPWNPNFAMWPLGLAVIAAWAACQGHRWSLAVAGLAASVAVQS
                     HLAFIAPGAAAFAAGLAGTAIVWRRDSRRLRVELAATAIALSVANAQLLVDQFFGTGN
                     LGRLLSARDAGAQRVSVHDVGAVLVSKLSPSVFRRDGWFAPVFPADRASPVAIVVGLS
                     ALAVLSIAAVAFSRSADDLDLAVDVDSTSPHRRNHRRLLATLFGIVGVTVVAGSVLAL
                     RYPMRIGIPLPYFRWVWPLGAILTGALAASAVRIGSERLAAPRGGRVTTGGRWPLAFG
                     AASVMVLTGVSGVPIGEEMSPNPQRYQDLAGPLSSAAIEALTRFDDGPIGSVFVEQSH
                     LQAGLWVIPALIDQLDAAGIDVAVSDPILDQQTGGRLTSDSAPHTQFRLILRGGLDST
                     DDLESNPGALRLATPVELADREIDEFHRLVDALAPVISDGANFRADADIVDAEGLALV
                     ESLRTDPPDPQYFFSAPSIRDA"
BASE COUNT          351 a          849 c          795 g          412 t
ORIGIN      
        1 gggtccacga ctgggtgatc ggcccgatcg cccaaccggg gacatagata tgcaccagcg
       61 ccatcgtcgc gaccagccgc tggggcgagg gcgccgcgcc ggcgccgaac gcgagggtcg
      121 ccgccagcac gacccagtag agcggaaaga tccgagcgaa gcgccgcaag gtgaacgtgg
      181 cgagatccgg cgggtcggtg ttgcgcacca gcgaacgcac gaatggtcgc ccgacgagca
      241 ggcctgacaa cacgaagaac accggcacgc caacgtcgag gcgcggcagg atcagtcgaa
      301 gccacgagtc ccaacgttcg gggttgacca cgccggtcac gaacgccacg tgggagatca
      361 acaccgcgat cgccgccagc acgcgagcga tgtcgaggcc gaaactgcgc tcgctgcctg
      421 ctgtgcggtc aactgtcaca tcgctagtgt ggaccaattc gaggacctga tggctgacgc
      481 tgaccgaccg cacccgactt cccccccgga cgaaccggtc gagcgcagcg atcgctcgct
      541 tgcgctcgat gcggtcgatg gccccggtcg agcccggcgg ggcaccctcg accccgtgcg
      601 atggacgatg gtggtgttga tcgccctggt cgtcgggctg ggggcggcgg cgtcgatccg
      661 cggatggatt ccggtcggag acaacgcgtt cttgtcgtac cggagccgcc atgtcgggtc
      721 gagcgatccg atcctgctga acaatgcgtc ctcgggcggc accgacaact ccgagccgta
      781 caaccatccg ggggcgttcc cactgaccct gatggccccc atcacactca tcggtggccc
      841 cggttcggta ccactcgctg cggcggtctg caccgcagcg tcgatcgggc tcatctcctc
      901 aaccgtgcga cgcgtcgccg gacgtggggc acagctgtgg tccctcgtcg tctcggcggg
      961 gttgctggcc acccagtcgg tcacgatgtt cgcggatccg tggaatccca acttcgccat
     1021 gtggcccctc ggcctggcgg tgatcgctgc gtgggcggcg tgccagggcc atcgttggtc
     1081 gttggcggtt gccggacttg ccgcatccgt cgccgttcag agccatctcg cgttcatcgc
     1141 tccgggcgct gccgcgttcg ctgccgggtt ggcgggaacc gcgatcgtgt ggcgtaggga
     1201 ttcccgccgg ttgcgcgtcg aactggccgc caccgcgatc gcgttgtcgg tcgccaatgc
     1261 gcagcttctc gtcgatcaat tctttggcac cggcaacctc ggccgcctgc tcagcgcgcg
     1321 agacgcgggg gcccagcggg tgagcgtgca cgatgtgggc gcggtgctcg tgtcaaagct
     1381 gtcgcccagc gtctttcgcc gtgacgggtg gttcgccccc gtgtttcccg cggatcgagc
     1441 ctcccctgtc gccatcgtcg tcgggctgag tgccctggcc gtgttgtcga tcgccgcggt
     1501 cgccttcagc cgttccgccg atgacctcga cctcgcggtc gacgtcgaca gcacctcgcc
     1561 gcaccgtcgc aaccatcgtc gtctcctcgc gacgcttttc ggcatcgtgg gggtgaccgt
     1621 ggtcgccggg tcggtcctgg cgttgcgcta cccgatgcgg atcggaattc cgttgccgta
     1681 tttccggtgg gtctggccgc tcggcgccat cctcaccgga gcgctcgccg cctcggcggt
     1741 gcgcatcgga agcgagcgcc tcgccgcccc tcgtggcggg cgggtgacca ccggtggccg
     1801 ctggccgctg gccttcggcg ccgcgagcgt catggtgctc accggcgttt cgggggttcc
     1861 catcggcgag gagatgtcgc ccaatcccca gcggtaccag gacctcgcag gaccgctgtc
     1921 gtctgcggcg atcgaggccc tgacccggtt cgacgacggg ccgatcggat cggtcttcgt
     1981 cgaacagagc caccttcagg cgggactgtg ggtcattccc gcgctgatcg accaactcga
     2041 tgccgccgga atcgacgtcg ccgtttccga cccgattctc gaccaacaaa ccggcggacg
     2101 actcaccagc gactcggcac cccacacgca gtttcggctg atactgcgcg gaggtctcga
     2161 ctccaccgac gacctcgaat ccaaccccgg agccctccga ctcgccaccc ccgtggagct
     2221 tgccgaccgc gagatcgacg aattccaccg cctggtcgac gcgctcgcac cggtgatctc
     2281 ggacggggcg aactttcggg ccgacgccga catcgtcgac gcggaaggac tcgccctggt
     2341 cgagtcgctg cgaaccgacc cgcccgatcc tcagtacttt ttcagcgctc cgtcgattcg
     2401 cgacgct
//
LOCUS       DAOOMA010000442         2406 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_54530_length_2406_cov_3.621438, whole genome shotgun sequence.
ACCESSION   DAOOMA010000442 DAOOMA010000000
VERSION     DAOOMA010000442.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 2406)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2406)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2406
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54530_length_2406_cov_3.621438"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..497)
                     /gene="pyrE"
                     /locus_tag="PLP95_10900"
     CDS             complement(<1..497)
                     /gene="pyrE"
                     /locus_tag="PLP95_10900"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR00336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HPB46352.1"
                     /translation="MTTAKDRLVRHLLDHSVKHGDFTLKSGRKSPYFLDSKQTACDPD
                     GVQLMAEVALEFIPPDATAIGGITVGADPVAYGIAAVAATKGRRLRSFTIRKEAKDHG
                     VTGRVAGALRPGDKVVITEDTTSRGVTIADAVETVRSFGAEPVLVLALVDRGGTAEGY
                     ATAAGV"
     gene            complement(494..1549)
                     /gene="purM"
                     /locus_tag="PLP95_10905"
     CDS             complement(494..1549)
                     /gene="purM"
                     /locus_tag="PLP95_10905"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HPB46353.1"
                     /translation="MNDNSTAQQGATYEAAGVSIHAGDEAVARIKNSVRSTFRPEVLG
                     DIGGFGGLFALGTRYRDPVLVSTTDGVGTKAMVATAAGRFDTIGLDLVAMCVDDLVCQ
                     GAEPLFFLDYISIGKLDPDHVAKLVDGVAEGCRQAGCALIGGEMAEHPGAMDPGEFDL
                     VGFAVGVVERTEIIDGSRVAPGDVLIGLHSPGLRSNGYSLARHVVFDVAGMSLEDPAW
                     DAQDAPSLADELLVPSVIYAPTVRELGSQVEIHSVAHITGGGIGGNLNRVLNDHTDAI
                     VERGSWEPPRIFAEIQRLGSISDEEMAKVFNLGIGMIVTIPESESQRALEVLESLGQR
                     AAVIGRIVEGTGAVVLS"
     gene            complement(1546..>2406)
                     /locus_tag="PLP95_10910"
     CDS             complement(1546..>2406)
                     /locus_tag="PLP95_10910"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HPB46354.1"
                     /translation="LDIIGADFVREIDPGEMVVIDRDGVRSLRVFPEERIDPTLCLFE
                     FVYFARPDSTLYGRSVHAARVRMGERLAQQSPVEADLVMGVPESGVPAAEGYARHSAI
                     PFGQGLVKNRYIGRTFIAPTQDMRSMAVRMKLNPLRENIAGKRLVVVDDSIVRGTTTR
                     AMIQMLREAGALEVHLRISSPPYRWPCFYGMDTGTRSELLAANMTVDEIRDYLGVDTL
                     AYLSLDRLIEATGATNAGYCTACLTGNYPVPVPVELSTAAGSTSDLSSADERGDHRIV
                     DVDVAAGPKP"
BASE COUNT          438 a          804 c          749 g          415 t
ORIGIN      
        1 acgcctgcgg cggtggcgta accctccgcg gtaccgcccc ggtcgacaag cgccagcacc
       61 aacactggct cggctccaaa ggagcgtacg gtctccaccg cgtcagcgat ggtgacgcca
      121 cgagacgtcg tgtcctcggt gatgaccacc ttgtcccctg gccgcagcgc cccggcaact
      181 cgaccggtga cgccatggtc ctttgcctcc ttgcggatgg tgaaactacg cagacggcga
      241 cctttcgtgg cggctactgc cgcgatcccg taggccaccg ggtcggcccc caccgtgatg
      301 ccgccgatgg cggtggcgtc gggcgggatg aactcgagtg cgacctcagc catgagctga
      361 accccatcgg ggtcgcacgc ggtctgtttg gagtcgagga agtacggcga tttgcgtccc
      421 gacttgaggg tgaaatcacc gtgtttgacc gaatggtcga gcaggtgccg aacgagacgg
      481 tctttggcgg tcgtcacgag agcaccaccg caccggtgcc ttcgacgatc cgcccgatca
      541 ccgccgcccg ctggccgagc gattcgagca cctcaagcgc tcgctgcgac tcactttcgg
      601 gaatggtcac gatcatgccg atacccaggt tgaagacctt cgccatctcc tcgtcggaga
      661 tggacccgag ccgctgaatc tccgcgaaga tccgcggtgg ctcccacgac cctcgctcga
      721 cgatggcgtc ggtgtggtcg ttgagtaccc gattcaagtt gccgccgatc ccaccgccgg
      781 tgatgtgggc gacggagtga atctccacct gcgaacccag ttcacgaacc gtcggcgcgt
      841 agatcaccga gggaacgagc aactcatccg caaggctcgg ggcgtcctgc gcgtcccatg
      901 ccgggtcttc gagcgacatg cctgcgacat cgaacaccac gtggcgagcc agcgagtagc
      961 cgttggaacg aagccccggc gagtgcagtc cgatcaacac gtcgccgggt gccactcggg
     1021 atccgtcgat gatctcggtt cgttccacga cgccgacggc aaacccgacc agatcgaact
     1081 ctcccggatc catggccccc gggtgctcgg ccatctcacc gccgatcaga gcgcagcccg
     1141 cctggcgaca accttcagcg accccgtcga ccagtttggc gacgtggtcg gggtcgagtt
     1201 tgccgatgct gatgtagtcg agaaagaaca gcggctcggc gccttggcac accaggtcgt
     1261 cgacacacat ggccacgaga tcgagtccga tcgtgtcgaa gcggcccgcg gcggtcgcga
     1321 ccatcgcctt tgtgccgacg ccgtcggttg tcgacaccag caccgggtcg cggtacctgg
     1381 tcccgagggc gaagaggcct ccgaacccgc cgatgtcgcc gagcacctca ggtcgaaacg
     1441 tcgaacgcac cgagttcttg atgcgagcta ccgcctcgtc gccggcatga atcgacacgc
     1501 ctgccgcttc gtaggtggca ccttgctggg ccgtggaatt gtcgttcatg gcttcggtcc
     1561 ggcggcgaca tcgacgtcga cgatccggtg atcccctcgt tcatcggccg acgaaaggtc
     1621 cgaggtcgac ccggccgcgg tcgacaactc cactggaacg ggaaccggat agttcccggt
     1681 gaggcaggcg gtgcaatacc cggcgttcgt cgcaccggtt gcctcgatca gccgatcgag
     1741 ggacagatac gccaaggtgt cgacgccgag gtagtcacgg atctcgtcga ccgtcatgtt
     1801 cgcggcgagc aactccgatc gggtgcccgt gtccatgccg tagaaacacg gccagcgata
     1861 cggcggcgaa ctgattcgca ggtgtacctc gagcgctccc gcctcacgaa gcatctggat
     1921 catcgctcga gtcgtcgttc cccgaacgat cgaatcgtcg accaccacca gacgtttgcc
     1981 ggcaatgttt tcgcgcaacg gattgagttt catccgcacc gccatcgacc gcatgtcctg
     2041 ggtcggggcg atgaacgttc ttccgatgta gcggttcttg accaggcctt gtccgaacgg
     2101 aatggccgaa tggcgggcgt atccttccgc cgccggcacc cccgactcgg gaacccccat
     2161 gacgaggtcg gcttcgacgg gcgattgttg ggcaaggcgc tcgcccatac gaacccgggc
     2221 ggcgtggaca cttcgcccgt acaacgtcga gtcgggtcgg gcgaagtaca cgaactcgaa
     2281 caagcaaagc gtcggatcga tccgttcctc aggaaacacc cgcaacgagc gcacgccgtc
     2341 gcgatcgatg accaccattt cgccggggtc gatctcgcgc acgaaatcgg cgccgatgat
     2401 gtcgag
//
LOCUS       DAOOMA010000443         2403 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_54621_length_2403_cov_4.747019, whole genome shotgun sequence.
ACCESSION   DAOOMA010000443 DAOOMA010000000
VERSION     DAOOMA010000443.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 2403)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2403)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2403
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54621_length_2403_cov_4.747019"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..587
                     /locus_tag="PLP95_10915"
     CDS             <1..587
                     /locus_tag="PLP95_10915"
                     /inference="COORDINATES: protein motif:HMM:NF019421.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HipA domain-containing protein"
                     /protein_id="HPB46355.1"
                     /translation="DQSAYVVRRFDREPSVGPLAARVHMEDLAQSLGRSPEGKYDVTA
                     REVVSLLRTIDTGGGVTSEFLRQLAFNVIVGNADAHAKNYSLYLRPDGVAMTPLYDLV
                     PVFLFPEVDQRLSMAIGGARSAKAVTPNHWRKFAQSVGLDVDEVLSLVREVAGAIGEA
                     CSGDNIWAALDPDQQRVATQYVARSVERSLQPIR"
     gene            complement(611..1756)
                     /locus_tag="PLP95_10920"
     CDS             complement(611..1756)
                     /locus_tag="PLP95_10920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HPB46356.1"
                     /translation="MNDVAIIGVGLHPFGRFGAKSAIDMGADAARAALADAGVAWSDI
                     QFGFGGSFEVDNPDAVTSRLGLTGIPFMDVYNGCATAATALNLAADSIRSGQYDIGIA
                     VGMDKHGYGAFTSDPVDYAAPEWYGEIGHFLTTKFFAMKINRYMHDHGISHETLARVA
                     NKGYRNGVLNPNALRRKEISVEAILSSRMLNYPLTQYMFCSPDEGAAAVIMCRADRAK
                     QYTSTPIYLRATTLRTRRYGAFEVHASWAAVEQDAAPTVYASKAAYEIAGIGPEDVDV
                     IQLQDTDAGAEVIHMAENGFCADGDQERLIAEGATEIGGSMPVNTDGGLIANGEPIGA
                     SGLRQVYELVQQLRGTAGDRQVPGDPKVGYAQLYGAPGTAGVSILSR"
     gene            complement(1758..2189)
                     /locus_tag="PLP95_10925"
     CDS             complement(1758..2189)
                     /locus_tag="PLP95_10925"
                     /inference="COORDINATES: protein motif:HMM:NF013920.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold domain-containing protein"
                     /protein_id="HPB46357.1"
                     /translation="MSQVAFAPEVFTWPSDSPQLISGKCEQCASVTFPKPSSCARCGS
                     VDVAEHLLARTGTLWTFSTQEFLPKEPYAGGETPETFRPYGVGLVQLGDEVRVEARLT
                     ESDPKRLQIGMELELVIVPFRTDPDGTTVMTFAFAPIDKEN"
BASE COUNT          390 a          763 c          806 g          444 t
ORIGIN      
        1 ccgatcaaag cgcgtatgtc gtgaggcggt tcgatcgtga gccctctgtg gggccacttg
       61 cagctcgcgt gcacatggag gatctcgccc agagcctcgg tcgttcgccg gaaggcaagt
      121 acgacgtgac ggcacgagag gtcgtttcgc tgctccgaac aatcgacacc ggcggtggcg
      181 taacgtcgga gttcctccgc cagttggcgt tcaacgtcat cgtcgggaac gcggatgccc
      241 acgccaagaa ctactcgctg tatctacgcc ccgatggggt tgcgatgaca ccgttgtacg
      301 accttgtgcc ggtattcctc tttcctgagg tcgaccaacg actctccatg gccatcggtg
      361 gggcgcggtc ggcgaaggcg gtgactccga accactggcg caaattcgca cagagcgtcg
      421 gactcgatgt cgacgaggtg ttgtcgctcg ttcgtgaggt ggccggtgcg atcggcgaag
      481 cctgcagcgg cgacaacatc tgggcagcgc tcgatcccga tcagcagcgc gtggcgacgc
      541 aatacgttgc ccgatctgtc gagcgctcgc ttcaaccgat tcgctagcga ctgctccgct
      601 cggccggccc tcagcgagac aggatcgaca ccccggccgt gccgggagcg ccgtagagct
      661 gtgcgtatcc gaccttcggg tcgcccggaa cctggcggtc gcccgccgtg ccgcggagct
      721 gttgcaccag ctcgtacacc tgacgaaggc ccgacgcgcc gatcggttcg ccgttggcga
      781 tgagcccacc atcggtgttc acgggcatcg acccgccgat ctcggtcgca ccctcggcga
      841 tgagccgttc ctgatcacca tccgcacaga acccgttctc ggccatgtgg atgacctcgg
      901 ccccggcgtc ggtgtcctgc aactggatga cgtcgacatc ctccggcccg attccggcga
      961 tctcgtacgc ggcctttgat gcgtacacgg tcggtgccgc gtcctgttcg acagcagccc
     1021 agctcgcgtg cacctcgaag gcgccgtagc ggcgggtcct cagcgtcgtg gcgcgcagat
     1081 agatcggcgt cgaggtgtac tgcttcgccc gatccgcccg gcacatgatg accgcggcgg
     1141 cgccctcgtc gggactgcag aacatgtact gggtcagcgg gtagttcaac atgcgcgacg
     1201 acaagatggc ctcgacgctg atctccttac gtcgcagcgc gttcgggttc aacacgccgt
     1261 ttcgataccc cttgttggcg acccgggcca gggtttcgtg gctgatcccg tggtcgtgca
     1321 tgtagcggtt gatcttcatg gcgaagaact tggtggtgag gaagtggccg atctcgccgt
     1381 accactcggg ggccgcgtag tcgaccgggt cggaggtgaa cgccccgtag ccgtgcttgt
     1441 ccatgccgac cgcgatgccg atgtcgtatt gacccgatcg aatcgagtcg gccgcaaggt
     1501 tgagcgcggt cgcggcggtg gcgcatccgt tgtagacgtc catgaacggg atgccggtga
     1561 gcccgagtcg actcgtcacg gcgtcgggat tgtcgacctc gaagctgccg ccgaagccga
     1621 actggatgtc ggaccaggcc accccggcgt cggcgagcgc ggcccgcgcc gcgtccgcgc
     1681 ccatgtcgat cgcggacttg gcgccgaagc gaccgaacgg gtgcaggccg accccgatga
     1741 tggcgacgtc gttcatctca gttctcctta tcgatgggag cgaaggcgaa ggtcatgacg
     1801 gtcgtaccgt cggggtcggt tcggaagggc acgatgacga gttcgagttc catgccgatc
     1861 tgcagacgct tcgggtcgct ttcggtgagc cgcgcctcga cgcggacctc gtcgccgagt
     1921 tgcacgagtc cgaccccata ggggcggaag gtctccgggg tctcgccgcc ggcgtagggc
     1981 tccttgggca ggaactcctg ggtgctgaag gtccacaacg tgccggtgcg ggccaacagg
     2041 tgctcggcga cgtcgaccga gccgcagcgc gcacatgagc tcggcttggg aaaggtcacc
     2101 gaggcgcact gctcacactt gccgctgatg agttgtggcg aatccgacgg ccaggtgaac
     2161 acctcggggg cgaacgcgac ctgcgacatg ggacctcctc gtggactgca tcggcgtttc
     2221 ctcaaaattg acgtcgatgt cgaatatctc gttcgaagtg tagagccgtg gtgggtgtcg
     2281 gtgcacagga gatggctcgc tcggcgattg ctcaggtcgc gctgctgcgc aggtacaccg
     2341 tcgccgccat tgcaacgtgc cacagcgcgt gcggctgcac gaggctgtcg ggccgacacc
     2401 acg
//
LOCUS       DAOOMA010000444         2403 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_54633_length_2403_cov_3.206559, whole genome shotgun sequence.
ACCESSION   DAOOMA010000444 DAOOMA010000000
VERSION     DAOOMA010000444.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 2403)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2403)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2403
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54633_length_2403_cov_3.206559"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1406
                     /locus_tag="PLP95_10930"
     CDS             <1..1406
                     /locus_tag="PLP95_10930"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012403.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HPB46358.1"
                     /translation="AEKHGEVIEVESDEYFDELRARLSDPDALVELAAPPGLRAQLRP
                     YQRRGMSWMAELCRIGLGGILADDMGLGKTLQVIALHLTLAAEGWAPTLVIVPTSVLG
                     NWRREFEKFAPDTPVRVVSGSSDSLEAPADGGVVVMSYGVARRNADRLARMRWGLVVA
                     DEAQHIKNPRSRVARALREIPSRARIALTGTPVENRLTELWSVMDWAAPGLLGSAERF
                     RTAYVVAIEKHRSPLAAERLRERIAPFMLRRLKTDPGVADDLPPRIHSDVTVTLSAEQ
                     LSLYEALVRESLAEIARAEGPKRSSMVFRLLTALKQIANHPAHYLHEADGVLAGRSAK
                     LEAACDLVTDAVDNDRQVLVFSQYVEMCSLLQRRFAEQHVHSEVLHGGLPAAARDALV
                     ARFQAGELGVLIISLKAGGTGLNLTAASQVIHFDRWWNPAVEDQATDRAWRIGQRETV
                     IVHRLIAAGTIDEQSRR"
     gene            1403..1546
                     /locus_tag="PLP95_10935"
     CDS             1403..1546
                     /locus_tag="PLP95_10935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB46359.1"
                     /translation="MIERKRDLATSIVGASEEAWITELDDDALGELVELRSESCESME
                     VLR"
     gene            1543..>2403
                     /locus_tag="PLP95_10940"
     CDS             1543..>2403
                     /locus_tag="PLP95_10940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB46360.1"
                     /translation="MSAETTGIGSRWMERLVAEGLATSGDVSRGRGMNTRGALHEVSV
                     EPGLVSASVAGRGSEPRRASVGFARVANEDWTAVAAVVRSDARWQVAVVEGRLPDDLL
                     EAAAEAGVTMWLDSATTSVDCSCERGGTLCTHALALCDHIATMLDDDPLLVVTLRNGD
                     RGAFIEGAVGVTGVGGGGVGGGGASTALASAKAESTDAATSGGGALVVTPRGAGVDVV
                     GGVVGSGGFRSGVVSPILTRGDAEFVDAAPAWRRSPSVSNWEPSTRRLVGRSRLAATP
                     PPRRRLPVRDR"
BASE COUNT          381 a          754 c          869 g          399 t
ORIGIN      
        1 gtgccgagaa acacggcgag gtcatcgagg tcgaatccga cgagtacttc gacgagttgc
       61 gtgcccggct cagcgatccg gatgcgctgg tggaactggc tgcgccgccc gggctgcgcg
      121 cgcagctccg gccctatcag cggcgcggga tgtcgtggat ggcggagttg tgccgcatcg
      181 ggctgggagg gatccttgcc gacgacatgg ggctcggaaa gaccctccag gtcattgcgt
      241 tgcacctgac cctcgcggcc gagggttggg cgccgaccct ggtcatcgtg ccgacgtcgg
      301 tgttgggcaa ctggcgccgt gagttcgaaa agtttgctcc ggataccccg gttcgggtgg
      361 tctccggctc ctctgacagt ctcgaggcgc cggccgacgg cggtgtcgtg gtgatgagct
      421 acggggtcgc tcgccgcaac gccgatcgtc tggcgcggat gcgctgggga ctcgtcgtgg
      481 ccgatgaagc gcagcacatc aagaacccgc gtagccgcgt tgcgagggcg ctgcgggaga
      541 tcccgagccg cgcacggatt gcgctcaccg gaactccggt cgagaaccga ctcaccgaac
      601 tctggtcggt tatggactgg gcggcaccgg gtctgctcgg ctcggccgag agattcagaa
      661 cggcctatgt cgttgcgatc gaaaagcacc gaagcccgct ggctgccgag cgcctccggg
      721 agcggatcgc accgttcatg ttgcgtcggc tcaagaccga tccgggggtt gccgacgatc
      781 tgcccccgcg aatccattca gacgtcacgg tgacgctcag cgccgaacag ctgtcgttgt
      841 acgaggccct cgtgcgcgaa tcgctcgccg aaatcgcacg cgccgaaggg ccgaagcgat
      901 cgagcatggt gtttcgactg ctcaccgcgc tcaagcagat cgccaatcat ccggcccact
      961 accttcacga agccgatggg gtgctcgccg gccggtccgc caaactcgag gcggcctgcg
     1021 acctggtgac cgatgccgtc gacaacgacc gccaggtact ggtgttttcc cagtacgtcg
     1081 agatgtgttc gctgctccag cggaggttcg ccgaacaaca cgttcattcc gaggtgctgc
     1141 acggtgggtt gccggctgcg gcgcgcgacg cgctcgtcgc cagattccag gcgggcgagc
     1201 tcggcgtgct catcatctcg ctgaaagcgg gcggcaccgg gctcaaccta accgcggcat
     1261 cgcaggtcat ccacttcgac cgttggtgga accccgccgt cgaagaccag gccaccgacc
     1321 gggcctggcg aatcggccaa cgagaaacgg tcatcgttca tcgcctcatc gccgcgggca
     1381 cgatcgacga gcaatcgcgg agatgatcga acggaagcgt gatctcgcaa cctcgatcgt
     1441 cggagcgagc gaagaggcgt ggatcaccga actcgacgac gacgcgctgg gtgaactcgt
     1501 cgagttgcga tccgaatcct gcgaatcgat ggaggtgctt cgatgagtgc cgagacgacc
     1561 ggtatcggca gccgttggat ggagcggctc gttgcggagg gtctggcgac gagcggcgac
     1621 gtgagtcgcg gtcgtgggat gaacacccgt ggtgcgctcc acgaggtgtc ggtcgaaccc
     1681 ggtctggtga gcgcgtcggt cgccggccgg ggcagcgaac ctcggcgtgc cagcgtcggt
     1741 ttcgcccggg tggcgaacga ggactggacg gccgtcgcgg cggtggtgag aagcgacgcc
     1801 cgctggcagg tcgcggtggt cgagggccgc ctgcccgacg acctgttgga ggcggccgcg
     1861 gaggccgggg tgaccatgtg gctcgactcg gcgacgacct cggtcgactg cagctgcgaa
     1921 cgcggcggca cgctgtgcac ccacgcgttg gcgctgtgtg atcacattgc gacgatgctc
     1981 gacgacgatc cgctgctcgt ggtcacgctg cgaaacggcg accgcggggc gtttatcgag
     2041 ggagccgtcg gtgtgacggg agttggaggg ggcggagttg gcgggggcgg agcttctacg
     2101 gcgctcgcat cagcgaaagc tgagtcgacc gacgccgcca cgtcgggcgg tggcgctctc
     2161 gttgtcaccc cccgaggcgc tggcgttgac gtcgtcggcg gcgttgtcgg atccggcggg
     2221 ttccgaagcg gggtggtgag ccccatcctc acccgcggcg atgcggaatt cgtcgacgcg
     2281 gcacctgcgt ggcggcgcag cccatcggtc tcgaactggg agccgtcgac gcggcggctg
     2341 gtaggtcggt cacgcctcgc tgcaactccg ccgccgcgtc gacggctccc agttcgagac
     2401 cga
//
LOCUS       DAOOMA010000445         2401 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_54711_length_2401_cov_3.462063, whole genome shotgun sequence.
ACCESSION   DAOOMA010000445 DAOOMA010000000
VERSION     DAOOMA010000445.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 2401)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2401)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2401
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54711_length_2401_cov_3.462063"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..488
                     /locus_tag="PLP95_10945"
     CDS             <1..488
                     /locus_tag="PLP95_10945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HPB46361.1"
                     /translation="FAFERDVGVVDTNIARVLARRCGRRLSQREAQREADEFVAAGHG
                     WAHNQSLMDLGNRLCRPRPDCEQCPLSSWCVWANDRGSLPDPAIGSAGVSTRQAPFEG
                     SDRQARGRLLSALQGGGVRAVDAHALMGFEGDLERARSIVDSLIRDGLVIVDGELLRL
                     P"
     gene            623..1465
                     /locus_tag="PLP95_10950"
     CDS             623..1465
                     /locus_tag="PLP95_10950"
                     /inference="COORDINATES: protein motif:HMM:NF024118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HPB46362.1"
                     /translation="MTLRITFYGVRGSTPCCCESNQRVGGNTACVVVEGDGFDPLVLD
                     LGTGLRFWGVDVLAAQSAPLRATALVSHLHWDHVQGLPFFPPIHQPGTEMHIIGPPQQ
                     GGSLEEVFKAFLCPPVFPVGLDDLVGIITFAEAGSEPIVHGTATITARDVPHVGPTVG
                     YRIDHGGRSIAYVSDHQQPGAGSTSVAEDVIALCDGVDVLIHDAQYTPAEFDERSDWG
                     HCTLDYALEVAVQSRVKALALFHHDPGHDDAMIDRLTSELVGRSPEGGPRIFTASEGL
                     VVDV"
     gene            1542..2138
                     /locus_tag="PLP95_10955"
     CDS             1542..2138
                     /locus_tag="PLP95_10955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB46363.1"
                     /translation="MTGPDGRRLRTDKSRQRVLDAVMSLMESTGEVPTAQQIADESAM
                     STRSVFRLFGDMQDLYSEAVRSRLVDLRGRYVAPSVELPLGERLAELVATRVSVHEEI
                     SPIRRVVAPKTRNPGELQEAFKSGMEWLRSQVAATFAPELAARAEHDRETVLNALDAV
                     LSWEYWDRLRSVQELEFAAAREVVARTVSSLVEAGGTA"
     gene            2203..>2401
                     /locus_tag="PLP95_10960"
     CDS             2203..>2401
                     /locus_tag="PLP95_10960"
                     /inference="COORDINATES: protein motif:HMM:NF013756.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase"
                     /protein_id="HPB46364.1"
                     /translation="MSDDVADIDPADYRKVLGAFPTGVTVVAAVADGRPGGLAVGSFF
                     SVSLEPPLVGFCVQKSSSSWAR"
BASE COUNT          384 a          741 c          844 g          432 t
ORIGIN      
        1 cgtttgcctt tgagcgcgac gtcggagtcg tcgacaccaa catcgcacgc gtcctggcgc
       61 gccggtgcgg ccgcaggtta tcccagcgcg aggcgcaacg cgaggccgat gagttcgtcg
      121 cggccgggca tggatgggcg cacaatcagt cgctgatgga tctcggcaat cgcctgtgtc
      181 gaccgaggcc ggactgcgag cagtgcccgc tgtcgtcgtg gtgtgtgtgg gcgaacgatc
      241 gaggttcgct cccagacccc gccatcggtt ccgcgggggt atcgacccgc caagcgccat
      301 tcgaggggtc ggatcgtcag gcacgaggac gtttgctgag cgcgctacag ggtggtggcg
      361 ttcgcgccgt cgacgcccac gcgctcatgg gcttcgaagg cgatctcgaa cgcgctcgga
      421 gcatcgtcga ttcgctgatt cgcgatggtc tcgtcatcgt ggacggcgaa cttctgcgcc
      481 tgccctgatc ggttggagcg accggcaatg gggtccggcc cacgccatga aatgggtcac
      541 agacccttca ggtgaacggg aggttgcgcc tagggttgaa ggaccggtcg ggggcgtgat
      601 cggagctgtg ccgaaggggt tggtgacact gaggatcacg ttctacgggg tcaggggttc
      661 gacgccgtgt tgttgtgaat cgaatcagcg ggtcgggggc aacaccgcat gtgtcgtggt
      721 cgaaggcgac gggttcgacc cgctcgttct cgacctcgga accggcctgc ggttctgggg
      781 cgtcgacgtc ttggcggcac aaagcgcgcc gttacgggct acggcactgg tgagtcacct
      841 gcattgggac catgtgcagg gtctgccgtt cttccctccg attcatcagc cgggcaccga
      901 aatgcacatc atcggtccgc cacaacaggg cggatcgctc gaggaggtgt tcaaggcgtt
      961 cctgtgcccg ccggtgtttc ccgtcggcct cgacgacctc gtgggcatca tcaccttcgc
     1021 ggaggcaggg agcgaaccga tcgttcacgg aaccgccacg atcacggcgc gagacgtgcc
     1081 acatgtcggt ccgaccgtcg gataccggat cgaccacggc gggcgaagca tcgcgtacgt
     1141 cagcgatcac cagcagccgg gcgccggttc gaccagcgtc gcggaggacg tgatcgcgct
     1201 gtgcgacggt gtcgacgtgt tgatccacga tgcccaatac accccggccg aattcgatga
     1261 gcgttccgac tggggacact gcaccctcga ctacgccctt gaggtcgcgg tccaatcgcg
     1321 ggtgaaggcg ctcgcgctct tccaccatga cccgggccac gacgacgcca tgatcgatcg
     1381 cctcacgtcg gaactcgtcg gccggtctcc agagggtggg ccccgaatct tcaccgcttc
     1441 cgaggggctc gtcgtcgacg tgtaatcggg ggcccaccgg cgtcgtccgg ggcgcatgac
     1501 gctcgtccga tgaggggatc ctcgctgata ccgtgacgga ggtgacaggt cccgacggcc
     1561 gacggctacg caccgacaag agtcgccaac gggtgctgga tgcggtgatg tcgctcatgg
     1621 agtcgaccgg cgaggtgccg acggcgcaac agattgccga cgagtcggcg atgtcgaccc
     1681 gatcggtctt tcggctcttc ggcgacatgc aggacctcta ctccgaagcc gtgcgctcac
     1741 ggcttgtcga cttgcggggg agatacgtcg ccccgtcggt cgaactccca ctcggcgagc
     1801 gccttgctga gctggtcgcg acacgggttt cggtccacga ggaaatctcg ccgattcgtc
     1861 gagtcgtggc gcccaagacc cggaatccgg gcgaactgca ggaagcgttc aaatcgggaa
     1921 tggaatggct gcgctcgcag gtggctgcga ccttcgcccc ggagctggcg gcgagggccg
     1981 aacacgatcg cgagaccgtg ctgaatgcgc tcgacgcggt cttgagttgg gagtactggg
     2041 atcggttgcg atcggttcag gaactcgagt tcgctgcggc gcgggaggtc gtggcgcgaa
     2101 ccgtgtcgtc gttggtcgag gcgggcggga ccgcgtagat cggcggtaac gttgcggcgc
     2161 acgacgccga agcgtcgcag cgttcgccat cgaggagacc acgtgtccga cgacgtcgcc
     2221 gatatcgacc ccgccgacta ccgcaaggtg ctgggggcct ttcccaccgg ggtgacggtc
     2281 gttgccgccg tcgcggacgg acggcccggc ggcctcgccg tgggatcgtt cttctcggtg
     2341 tcgctcgagc caccactggt cgggttctgc gtccagaaat cgtcgagttc gtgggctcgg
     2401 a
//
LOCUS       DAOOMA010000446         2399 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_54769_length_2399_cov_3.507679, whole genome shotgun sequence.
ACCESSION   DAOOMA010000446 DAOOMA010000000
VERSION     DAOOMA010000446.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 2399)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2399)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2399
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54769_length_2399_cov_3.507679"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            145..1035
                     /gene="dapA"
                     /locus_tag="PLP95_10965"
     CDS             145..1035
                     /gene="dapA"
                     /locus_tag="PLP95_10965"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HPB46365.1"
                     /translation="MSLPTARFGRVITAMVTPFDDSGALDLDAAANLAAWLVDNGSDG
                     LVLAGTTGESPVLSDSEEVALAKAVRAAVDVPLLLGTGSNDTNYAVEATRRAAGLGVD
                     GVLVVSPYYNRPPQSGLDHYFRSVAAATDLPVLLYDVPTRTGRKVATATMLRLAHEVD
                     NIVGLKDAAGNPAETARLMRDAPDDFELYSGDDALTLPLLAIGAVGAVSVASHWAGRQ
                     IGEMIAAFLRGDHGEAIRINGGLIESYEFAGSDDAPNPMPSKAILRVLGIKVGNCRAP
                     LSFAPDWLEPRAREVLSALA"
     gene            1107..>2399
                     /locus_tag="PLP95_10970"
     CDS             1107..>2399
                     /locus_tag="PLP95_10970"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020376962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HPB46366.1"
                     /translation="MASSDVRITFLGGLGEIGRNCAAIEIDSRIMLLDCGLMFPDTDM
                     LGIDVVLPDFTWLRERADDIEGCILTHGHEDHVGALSYALREFSFPIIGSEFTLGLAR
                     GRIEEAGLLKRTDLVVVRDNERRTFGPFDCEFLPTTHSVPHAFATVFHTPQGAIVHSG
                     DFKIDLTPVDGRLADLARIGGIASGDGIRLLLSDSTNAEEHGHSRSETAVGGVLYDLM
                     HQHHGRRIITACFASHIHRVQQLADAAQSFGRVIVPLGRSMRRNISMARELGILNIPD
                     HALGDIENINDYEDGKVCVVCTGSQGEPMSALALLGTDDNRWLKVTSNDTVILSSHPI
                     PGNESAVSRVIDNLIRTGAQVVHSGIADVHATGHAKAEELKLFLSVTRPEWFVPVHGE
                     YRHLVAHARLGEVMGVEKDHVLVAEDGDSLILDDDGLRF"
BASE COUNT          396 a          768 c          784 g          451 t
ORIGIN      
        1 gatgcaaggg atctcggcgg gcaacctcga ctggttcgtc aagccgacga tcgactcgta
       61 tcacagcgtc tggttcgagt tgcacgagga cctgctggta actctgggga tcgaccgttc
      121 ggctgagcgg tagcctgtgc gtccatgagt ctgcctactg ctcgcttcgg acgggtcatc
      181 accgcgatgg tcacaccgtt cgacgactcc ggagccctcg atctcgatgc tgcggccaac
      241 ctggctgcgt ggctcgtcga caacggaagc gacgggctgg tcctcgccgg aacgaccggt
      301 gaaagccccg tcctcagcga ttccgaggag gtggccctcg ccaaggcggt gcgtgccgcg
      361 gtcgacgtgc ccttgttgtt gggaaccgga tcgaacgaca cgaattatgc ggttgaagcc
      421 acccggcgcg cggccggtct gggcgtcgac ggggtgctcg tcgtgagccc ctactacaac
      481 cgcccgcccc aatccggcct cgaccactat ttccggtcgg tggcggcggc gacggatctg
      541 ccggtgttgc tctacgacgt tccgacgcgc acgggccgaa aggtggcgac cgccacaatg
      601 ctgcgtctcg cccacgaggt cgacaacatc gtcgggctca aagacgcggc gggaaacccg
      661 gccgagacgg ctcgcctgat gcgcgacgcg cccgacgatt tcgagctgta ttcgggcgac
      721 gatgccctca cgttgccgtt gctggcgatc ggcgcggtcg gtgcggtgag cgttgcgagc
      781 cattgggccg gccgccagat cggcgaaatg atcgccgcgt tcctgcgcgg cgaccacggc
      841 gaggccatcc gcatcaacgg cgggctgatc gagagttacg agttcgcggg ttccgacgac
      901 gcgccgaatc cgatgccgtc caaagcgatc ctgcgggtgc tcgggatcaa ggtcggcaat
      961 tgtcgtgcgc cgctgtcgtt tgctcccgac tggcttgagc cccgcgctcg agaggtgctc
     1021 tcagcgttgg cctgagcagc gtccatctat ccgacctccc atctcgttgg cctgcgccaa
     1081 ccccatcgaa agctcaacct gactcaatgg cttcttcgga cgttcgtatt acgttcctcg
     1141 gcggactcgg cgaaatcggt cgcaactgtg cggcaatcga gatcgactct cgcatcatgt
     1201 tgctcgattg cgggctcatg tttcccgaca ccgacatgtt gggcatcgac gtcgtgttgc
     1261 ccgatttcac gtggttgcgt gaacgggccg acgacatcga ggggtgcatc ctcacccacg
     1321 gccacgagga ccatgtcgga gcgttgagct atgcgttgcg cgagttcagc tttccgatca
     1381 tcggttcgga gttcaccctc ggcctggcgc gtggccgaat cgaggaggcg ggcctcttga
     1441 agcgcaccga tctcgtcgtg gtgcgcgaca acgagcggcg aacgtttggc ccgttcgact
     1501 gcgagttcct tccgacgact cactcggtgc cgcacgcgtt cgccacggtg tttcacacgc
     1561 cacagggtgc gatcgtgcac tccggcgact tcaagatcga cctgaccccg gtcgacggca
     1621 ggctcgcaga tctcgctcgc atcggtggca tcgcgagcgg tgacgggatc cggctgttgc
     1681 tgagcgattc caccaacgcc gaggagcacg gccattcgcg ttcggagacc gcggtcggtg
     1741 gggtgctgta tgacctgatg caccaacacc acggccgccg catcatcacg gcgtgtttcg
     1801 cgtcgcatat tcaccgggtg caacagctcg ccgacgcggc acagtcgttc ggacgggtca
     1861 tcgtgccgct cgggcgttcg atgcgccgca acatttccat ggcacgggaa ctcggcattc
     1921 tcaacattcc cgaccacgcg ctcggcgaca tcgaaaacat caacgactac gaggacggca
     1981 aggtctgtgt cgtgtgcacc ggcagccagg gcgagccgat gtcggccctc gccctgttgg
     2041 gcaccgacga caaccgttgg ctgaaggtca cctcgaacga cacggtgatc ctgtcctcgc
     2101 acccgattcc cggcaacgag tcggcggtgt cgcgggtgat cgacaacctg attcgaaccg
     2161 gcgctcaggt ggtgcactcg gggatcgccg atgtgcatgc caccggccac gccaaggccg
     2221 aggagctgaa gctgttcttg tcggtgaccc ggccggagtg gttcgtgccg gtgcacggcg
     2281 agtaccggca cctggtggcc cacgcccgcc tcggcgaggt gatgggggtt gagaaggatc
     2341 acgtgttggt ggccgaggac ggagattcgc tgattctcga cgacgacggc ctgcggttc
//
LOCUS       DAOOMA010000447         2392 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_54989_length_2392_cov_3.909713, whole genome shotgun sequence.
ACCESSION   DAOOMA010000447 DAOOMA010000000
VERSION     DAOOMA010000447.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 2392)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2392)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2392
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54989_length_2392_cov_3.909713"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1036
                     /locus_tag="PLP95_10975"
     CDS             <1..1036
                     /locus_tag="PLP95_10975"
                     /inference="COORDINATES: protein motif:HMM:NF024091.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HPB46367.1"
                     /translation="SHRHPRGDLLGGRHMTSTTPPTAASAASAASAAGGATAARTQIL
                     DRGYRHYDGPRTGTSGAMRSVVKYTAQRALGIHRKFRYKIVPILVIAISFVPHIVWVG
                     IIVLTNRLDEQSDGVMPAGVGRMVANELVKDYPGSYGSIVLAIALFAAFVAPEVLCTD
                     RSSGMLGLYLASPLNRTRYLAAKAMAIGMLLSIITVGPALVMLTGYTSQGYGPDGFGD
                     WIVTLARVLGVGVAIAAFHTLIAAAISSITTRRAAASATFIVLVLGAGVLATLSLVEL
                     RAPMWVGAFDLVQLPSEFAYRVFGRPGPLSYWPRPPLDTWVVYAGFFGWMAVSLLVIW
                     DRYRRAEVTK"
     gene            1033..1965
                     /locus_tag="PLP95_10980"
     CDS             1033..1965
                     /locus_tag="PLP95_10980"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPB46368.1"
                     /translation="MTAPIIAFRDVSKSFKGLVAVSEVSFDIGPGVTALLGPNGAGKS
                     TVLRMLVGLTAPDRGTVSVFGHDPRSNTAVLRYVGLVPQQEGVFPRTRAIDFVRLAAV
                     LNSVDDPDAAARMALEAVELDPDDTRFVDQYSKGMRQRVKLAQAIVHGPSVIVADEPL
                     NGLDPRQRQRLIRIFQRLGAEGRCVIVSSHVLDEVERFGSQVLVLAQGRLAAVGDFRA
                     IRDLMDDRPHLIRLRSDNLRALTSALLARGIVRGVQFTDADTAEVAVDDVALFRRSIA
                     PAARHLDCSLTEVAPLDDDLESVFRYLVAGPGGR"
     gene            1962..>2392
                     /locus_tag="PLP95_10985"
     CDS             1962..>2392
                     /locus_tag="PLP95_10985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB46369.1"
                     /translation="MMSATSTNVALYRTLLSATTSRARVYGFVGVAVVQLIVSWMVRG
                     SGDRVQGALTMIDAFGLTLTIPLAALVFGTASLGDPIEDGTYVYLWLRPLRRAAISAA
                     AFASTLTLVVPLAVLPTVLSALVIAEGPRLVLAAAVASMAAA"
BASE COUNT          320 a          830 c          802 g          440 t
ORIGIN      
        1 atcgcatcgt caccctcgag gagatcttct tggaggtcgg cacatgacct ccacgactcc
       61 gcctaccgcc gccagcgccg ccagcgccgc cagcgccgcg ggtggcgcca ccgccgcccg
      121 cacccagatc ctggaccgcg gatatcgcca ctacgacggc ccgcgcaccg gaacctcggg
      181 cgcgatgcgc agcgtcgtca agtacacggc gcagcgtgcg ctcggcatcc accgcaagtt
      241 ccgctacaag atcgtgccga tccttgtgat cgccatcagc ttcgtccccc acatcgtgtg
      301 ggtcggaatc attgtgttga ccaaccgact cgacgagcaa tccgacggtg tgatgcccgc
      361 tggcgtcggt cgaatggtgg cgaacgagct cgtcaaggac tatccgggtt cctacgggtc
      421 gatcgtgttg gccatcgccc tgtttgccgc cttcgtcgct cccgaggtcc tgtgcaccga
      481 ccgcagctcg gggatgttgg ggctgtatct cgcctcgccg ttgaatcgga cgcgttatct
      541 ggccgccaag gcgatggcga tcggcatgtt gttgtcgatc atcaccgtcg gcccggcgct
      601 ggtcatgttg accggctaca cctcgcaggg atacggaccc gacggctttg gtgattggat
      661 cgtcaccctg gcccgggtgc tcggagtcgg cgtggccatc gccgcgttcc acaccctcat
      721 cgccgccgcc atcagttcga tcacgacccg tcgtgcggcg gcatcggcga cgttcatcgt
      781 cctggtgctg ggcgcggggg ttttggcgac gctgtcgctg gtggagttgc gcgcgccgat
      841 gtgggtcggc gccttcgacc tggtgcagtt gccgagcgag ttcgcctacc gcgtgttcgg
      901 ccgacccggt ccgctgtcgt attggccccg cccgccgctc gacacctggg tcgtctacgc
      961 cggattcttc gggtggatgg cggtgtcgtt gctcgtgatc tgggaccggt accggcgcgc
     1021 cgaggtcacg aaatgaccgc gccgatcatc gcctttcgcg acgtgtccaa gtcgttcaag
     1081 ggactcgtcg cggtgtcgga ggtgagcttc gacatcggac ccggtgtgac cgcgctgctc
     1141 ggacccaatg gcgccggcaa gtcgacggtg ttgcgaatgc tcgtcgggct gaccgcgccc
     1201 gatcggggga ccgtgtcggt gttcggccac gacccgcgct ccaacaccgc cgtgttgcgc
     1261 tacgtcggcc tcgtcccgca acaagagggg gtgtttcccc gcacacgggc catcgacttc
     1321 gttcgcctcg cggcggtgct caacagcgtc gacgaccccg acgccgcggc gcgcatggcc
     1381 cttgaagccg tcgaactcga ccccgacgac acccgattcg tcgaccagta ctccaagggc
     1441 atgcgccagc gggtcaagct cgcccaggcg atcgtgcacg gcccgtcggt gatcgtggcc
     1501 gacgagccgc tcaacggcct cgaccctcgc cagcgccaac ggctgatccg gatctttcaa
     1561 cggctcggcg ccgagggacg gtgcgtcatc gtgagcagcc acgtcctcga cgaggtggag
     1621 cggttcgggt cgcaggtgct ggtgctcgcc caggggcgcc tcgccgcggt cggcgacttc
     1681 cgggcgatcc gcgacctgat ggacgatcgg ccgcacctca tccggttgcg cagcgacaat
     1741 ctccgtgcgc tcacctcggc gctgttggcc cgcggcatcg ttcgcggcgt gcagttcacc
     1801 gacgccgaca ccgccgaggt cgccgtcgac gacgtcgcgt tgtttcggcg atcgatcgcc
     1861 cccgcagcgc ggcacctcga ctgttcactc accgaggtgg cgccgctcga cgacgacctc
     1921 gaaagcgtgt ttcggtacct cgtcgccggc cccggagggc gatgatgtcg gcgacctcga
     1981 cgaacgtcgc gctctatcga accctgctgt cggcgacgac gagccgggcg cgggtgtacg
     2041 gcttcgtcgg tgtcgccgtg gtgcaactga tcgtgtcgtg gatggtgcgg gggagcggcg
     2101 atcgcgtgca gggggcgctc acgatgatcg acgcgttcgg tctgaccctg accattccgc
     2161 tcgcggcact cgtgttcggc acggcatccc tcggtgaccc gatcgaggac gggacctatg
     2221 tgtatctgtg gttgcgtccg ctgcgccggg ccgcgatcag cgcggccgcc ttcgcctcaa
     2281 ccctcaccct cgtcgtgcca cttgcggtgc tgccgacggt gctgagcgcg ctggtcatcg
     2341 cggaggggcc gcgcctggtg ctggcggccg ccgtcgcgtc gatggccgcg gc
//
LOCUS       DAOOMA010000448         2390 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_55048_length_2390_cov_3.312206, whole genome shotgun sequence.
ACCESSION   DAOOMA010000448 DAOOMA010000000
VERSION     DAOOMA010000448.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 2390)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2390)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2390
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55048_length_2390_cov_3.312206"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..418
                     /locus_tag="PLP95_10990"
     CDS             <1..418
                     /locus_tag="PLP95_10990"
                     /inference="COORDINATES: protein motif:HMM:NF024591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HPB46370.1"
                     /translation="FTGYWRDPDATAKAFAGGWFHTGDIGVRDTDGFVYIVDRLKDMI
                     VSGGENIASSEVERAIYEHPAVVEAAVVARPDDRWGEVPVAFVVTSDEATTPDELIDH
                     CRARLAKFKVPTAVTLIEALPRNPSGKVLKRELRER"
     gene            complement(441..1346)
                     /locus_tag="PLP95_10995"
     CDS             complement(441..1346)
                     /locus_tag="PLP95_10995"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HPB46371.1"
                     /translation="MNELSFDDRVVVVTGAGRGIGRAYAELLAARGASVVVNDLGTSM
                     GGRGTDVAPANEVVAQIHDCGGVAVADGNDVSRVEGAEALVATALEHFGRLDALINNA
                     GIMMRATMPEIDLDTLQRHLDVHLIGSFNTARAAWPHFVEQGRGRIVMTTSAGMLGHP
                     ANLAYAAAKGGVVGLMRALAASGRGHDIAVNAVAPAAETRMAGQATDSPPDSPMAPEL
                     VAPLVAYLCHDACPVSGEIYAAGAGRFSRMVIATTEGYLGGHDTTIDDIAQNWAAIDD
                     TSTLSISADLLEWSAEFTRHLHAAD"
     gene            1412..2062
                     /locus_tag="PLP95_11000"
     CDS             1412..2062
                     /locus_tag="PLP95_11000"
                     /inference="COORDINATES: protein motif:HMM:NF036525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HPB46372.1"
                     /translation="MSEPTTMPEPSSPAEPSPPADERARSDKWLARRESIVDTSAKVF
                     AKQGYHATGIVELCDANGLGKGAFYHYIGSKEELLAAIHDRVMDEVLAGADRVIDGDG
                     SPATKLALLGDELLDVIHRYPDHVWVFLHEFPALTEERAATFRARRRRYEAAVESVLQ
                     AGIDAGEFRDLDPRLTTLAWLGMHNYTYLWLRGEKGVVAHDIAEPFADIFLRGILA"
     gene            complement(2083..2319)
                     /locus_tag="PLP95_11005"
     CDS             complement(2083..2319)
                     /locus_tag="PLP95_11005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB46373.1"
                     /translation="MQRQFHVSFQDDPVAVACRHVTGLSTIMWGNDYPHAEGTFRGSQ
                     ELLSSLMAGVPDEERKAMVGGTLADLLGFERTAA"
BASE COUNT          387 a          814 c          795 g          394 t
ORIGIN      
        1 gttcaccggc tattggcgtg accccgacgc gacggcgaag gcgtttgcgg gagggtggtt
       61 ccacaccggc gacatcgggg ttcgcgacac cgacgggttc gtctacatcg tcgatcgtct
      121 caaggacatg atcgtgtcgg gcggcgagaa catcgccagt tccgaggtcg agcgggcgat
      181 ctatgaacac cccgcggtcg tcgaggcggc cgtggtcgca cgacccgatg accgctgggg
      241 cgaggttccg gtcgcgttcg tggttacctc cgacgaggcg accacccccg acgaactcat
      301 cgaccattgc cgggcacggc tcgctaagtt caaggtgccg acggcggtga ccctcatcga
      361 ggcactcccc cgcaatccgt cgggcaaggt gctcaagcgt gagctgcgtg agcggtgagc
      421 tgcgtgagcg gtgaggctgg tcaatccgca gcgtggaggt gtctggtgaa ctcggccgac
      481 cattccaaca ggtcggccga gatgctcagc gtggacgtgt cgtcgatggc cgcccagttc
      541 tgcgcgatgt cgtcgatcgt ggtgtcatga cctccgaggt acccctcggt cgtggcgatg
      601 accatgcgcg agaaccggcc cgctccggcg gcgtagatct cgccgctcac cggacaggcg
      661 tcgtggcaga ggtaggcgac gagcggcgcc accaactccg gggccatcgg cgagtcgggc
      721 gggctgtcgg tggcctggcc cgccatgcgg gtttcggccg cgggggcgac cgcattcaca
      781 gcgatgtcgt ggccccggcc cgacgccgcg agcgctctca tcagcccaac gacgccgcct
      841 tttgccgcgg cgtatgccag gttcgctggg tgtcccaaca tgccggccga ggtggtcatg
      901 acgatgcggc cccgaccctg ttcgacgaaa tgtggccacg cggcgcgggc cgtgttgaac
      961 gagccgatca ggtggacatc caggtggcgc tgcagcgtgt cgaggtcgat ctcgggcatg
     1021 gtcgcccgca tcatgatgcc ggcgttgttg atgagtgcgt cgaggcgacc gaagtgttcg
     1081 agcgcggtcg ccacgagcgc ctcggcgccc tcgaccctcg acacgtcgtt gccgtccgcc
     1141 accgccaccc cgccgcagtc atggatctgt gcgacgacct cgttcgccgg cgcaacatcg
     1201 gtgccgcggc cgcccatcga ggtgccgagg tcgttcacca cgacgcttgc accgcgcgcc
     1261 gcgagcaatt cggcgtaggc ccgtccgatg ccgcgccctg cgccggtcac cacgaccacc
     1321 cggtcgtcga aggaaagttc gttcacgctg ccaaccccct gatgtaccag cgaccgtctc
     1381 gccgcgaacc cccgttaacg ttggagtcct gatgtccgag ccaaccacga tgccagagcc
     1441 atcctcgccc gccgagccat ccccgcccgc cgacgaacgc gcccggagcg acaagtggct
     1501 cgctcgccgc gaatcgatcg tcgacacctc ggccaaggtg ttcgccaagc agggctatca
     1561 cgccaccggc atcgtcgaat tgtgcgacgc aaacgggctc ggcaagggcg cgttctacca
     1621 ctacatcggc tcgaaggagg agctgctcgc agcgatccac gaccgggtga tggacgaggt
     1681 gctcgcgggt gccgatcggg tgatcgacgg cgacggatcc ccggccacga agctggcctt
     1741 gctcggcgac gaactgctcg atgtcattca ccgctacccc gatcacgtgt gggtgttcct
     1801 gcacgaattc ccggcgctca ccgaggagcg ggcggcgacg tttcgggcac ggaggcgacg
     1861 ctacgaggcg gccgtcgagt cggtgctgca ggccggcatc gacgccgggg agttccgcga
     1921 cctcgatccc cgactcacca cactcgcctg gctgggcatg cacaactaca cctacctgtg
     1981 gctgcgcggc gaaaagggcg tcgtcgccca cgacatcgcc gaacccttcg ccgacatctt
     2041 cctgcggggc atcctggcct gaggcaattc gggcgccgag gctcacgctg cggtgcgttc
     2101 gaagcccaac agatctgcga gggttccgcc caccatcgcc ttgcgctcct cgtcgggaac
     2161 tcccgccatc agcgatgaca gaagttcctg gctgccgcgg aaggtcccct ccgcgtgcgg
     2221 atagtcgttg ccccacatga tcgtcgacag gccggtgacg tgacggcacg cgacggcgac
     2281 cgggtcgtcc tggaacgaca cgtggaactg acgctgcacg tactcactcg gcatgagcgg
     2341 gtacagccat tactcgttga gcttgaacag ctgcgccatg ccgggttggt
//
LOCUS       DAOOMA010000449         2387 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_55148_length_2387_cov_3.823328, whole genome shotgun sequence.
ACCESSION   DAOOMA010000449 DAOOMA010000000
VERSION     DAOOMA010000449.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 2387)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2387)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2387
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55148_length_2387_cov_3.823328"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(145..1290)
                     /gene="sucC"
                     /locus_tag="PLP95_11010"
     CDS             complement(145..1290)
                     /gene="sucC"
                     /locus_tag="PLP95_11010"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HPB46374.1"
                     /translation="MDLFEYQGKQFFAKFGIPVSDGAATQDVDEAVAIADRVGYPVVI
                     KAQVHVGGRGKAGGVKLANNADEAREHAGNILGLDIKGHIVGTIWVEHASDISEEYYA
                     SFTLDRGAKKYLGMLSAEGGVEIEAVAEENPDAIAKIWIDPVDGLDEATARTWVEAAK
                     LNPAATDGAVDILLKLYNAYTEGDADLAEINPLILKPTGEVHALDAKVTLDGNAVFRH
                     PEYEEYDKTQNRDEREQAAHDKGLQYVGLDGSVGVIANGAGLAMSTLDVVKQAGGEAA
                     NFLDIGGGANADVMAGALEVINNDPRVKSIFINIFGGITRGEEVANGIITALGRVQID
                     APIVIRLDGTNADEGREILQPHLSDKLQMAPTMLGAAAKAVELAAAR"
     gene            complement(1457..2299)
                     /locus_tag="PLP95_11015"
     CDS             complement(1457..2299)
                     /locus_tag="PLP95_11015"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HPB46375.1"
                     /translation="MRIGLSFANLGPLASAEGAAALGEACDAHGIESLWTVEHPVVPA
                     NYDSEYPYDPSGKMPIPAEWPFPDPLVWLTWVAAHTKKVRLGTGVLILPLRNPVVLAK
                     EIATLDAMSGGRVELGIGVGWLEEEFAALGVPFERRGERTDEYIEVLRALWSPGPTSA
                     AGPIVRFDDMVSSPDPVQSSVPIVVGGHSHAAARRAGRLGDGFFPGRQDDLAELVSTM
                     RKTAREAGRDPDDIELTFAGSKSAEKRRQLEDLGASRLVYGTPMVAPDEMNAAIERLL
                     DAVT"
BASE COUNT          383 a          836 c          743 g          425 t
ORIGIN      
        1 ggtgcccgcc acgacttggg tgccgtaatc acggttcaac aggccgtagt aacgaccctg
       61 gcttccggtc aggccctgat agacgacctt ggtgttctca tcgatgaaga tcgacattgt
      121 tctgtctcct tgacggtcgt tcgatcagcg ggccgcggcc agctcgacag ccttcgcggc
      181 tgcacccaac atggtcggcg ccatctgcaa cttgtcgctc agatgcggct ggaggatctc
      241 gcgaccctca tcggcgttcg tgccgtcgag acggatcacg atcggcgcgt cgatctgcac
      301 gcggccgaga gcggtgatga tgccgttcgc cacctcttcg ccgcgggtga tgccgccgaa
      361 gatgttgatg aagatcgact tcacacgggg gtcgttgttg atgacctcga gcgcgccggc
      421 catcacgtcg gcattcgcgc cgccgccgat gtcgaggaag ttcgccgcct cgccaccggc
      481 ctgcttgacg acatcgaggg tcgacatcgc aaggcccgca ccgttggcga tcacgccgac
      541 cgaaccgtcg agaccgacgt actgcaggcc cttgtcgtgg gcggcctgct cgcgctcgtc
      601 gcggttctgg gtcttgtcgt attcctcgta ttccgggtga cggaacacgg cgttgccgtc
      661 gagggtcacc ttggcgtcga gcgcgtggac ctcaccggtc ggcttgagga tgagcgggtt
      721 gatctcggcg agatcggcgt cgccctcggt gtaggcgttg tagagcttca acaagatgtc
      781 gaccgcaccg tcggtggcgg ccgggttgag cttggcggcc tcgacccagg tgcgggcggt
      841 cgcctcgtcg agcccgtcga ccgggtcgat ccagatcttg gcgatggcgt cggggttctc
      901 ctcggcgacg gcctcgatct cgacaccacc ctcagccgac agcatgccga ggtacttctt
      961 ggcgccacgg tcgagggtga agctcgcgta gtactcctcg gagatgtcgg atgcgtgctc
     1021 gacccagatg gtgcccacga tgtggccctt gatgtcgagc cccaaaatgt tgcccgcatg
     1081 ctcgcgagcc tcatcggcgt tgttcgccag cttcacgccg ccggccttgc cgcgaccgcc
     1141 gacgtgaacc tgggccttga tcaccaccgg gtacccgacc cgatcggcga tcgcaaccgc
     1201 ttcgtccacg tcctgtgtcg ctgcgccgtc ggacaccgga atgccgaact tcgcaaagaa
     1261 ctgcttgcct tgatattcga acaggtccac gcctggtcct ccacctcgtc ggtgcgccgt
     1321 atgaatgcgg cctcatgcag accggcgttt cgttcgccgg cacgtcggtc gactttacat
     1381 gttcaagaca agtggcagaa acgccgggct ccgtcgagca gttgccggcc tgccgcccga
     1441 cgcgcgcaac gttcgctcag gtcacggcgt cgagtagacg ctcgatggcc gcgttcatct
     1501 cgtcgggtgc caccatcggg gtgccgtaca cgagacgact cgccccgagg tcctcgagtt
     1561 gacgccgctt ctcggcgctc ttggagccgg cgaaggtcag ttcgatgtcg tcggggtcgc
     1621 ggccagcctc gcgcgccgtc ttgcgcatcg tcgacaccaa ctcggccaga tcgtcctgac
     1681 gcccaggaaa gaacccatcg ccgagacggc cggcgcgacg cgcggcggca tggctgtggc
     1741 cacccaccac gatcggaacg ctcgactgca ccggatccgg gctcgacacc atgtcgtcga
     1801 aacgaacgat cggccccgcc gcgctcgtcg gccccggcga ccacaacgcc cgcaacacct
     1861 cgatgtactc gtcggtgcgc tcccctcgcc gctcgaaggg cacaccgagc gctgcgaact
     1921 cctcctcgag ccaccccaca ccgatgccca actcgacacg accccccgac atcgcatcga
     1981 gggttgcgat ctctttggcc agaaccaccg gattgcgcaa cggcagaatc aacaccccgg
     2041 tgccgaggcg gaccttcttg gtatgggccg ccacccaagt cagccacacc agcgggtcgg
     2101 gaaacggcca ctcggcggga atcggcatct tgcccgacgg gtcatagggg tactcggagt
     2161 cgtagttcgc cggaaccacc gggtgctcga cggtccacag cgactcgatg ccgtgggcgt
     2221 cgcacgcctc gccgagcgcc gccgcaccct cagcactcgc caggggaccc agattcgcaa
     2281 agctcaaacc aatccgcatg acaggaccct accgaccgcg ccgcctccgc gccccgtggt
     2341 ctgcgcttgg ctcgcggccg cttggcccgc cgccgcttgg ccagccg
//
LOCUS       DAOOMA010000450         2373 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_55612_length_2373_cov_3.548318, whole genome shotgun sequence.
ACCESSION   DAOOMA010000450 DAOOMA010000000
VERSION     DAOOMA010000450.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 2373)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2373)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2373
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55612_length_2373_cov_3.548318"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..317
                     /locus_tag="PLP95_11020"
     CDS             <1..317
                     /locus_tag="PLP95_11020"
                     /inference="COORDINATES: protein motif:HMM:NF014868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB46376.1"
                     /translation="AAIEIVFIAQEILVGNADYPTEAIGRNTRRFRQLGMGYTNLGAL
                     LMALGLPYDSDGGARLGRCGPGADVGPRLCDLGQARRPHGAIRRIPREPRAHASGARN
                     AP"
     gene            205..1614
                     /locus_tag="PLP95_11025"
     CDS             205..1614
                     /locus_tag="PLP95_11025"
                     /inference="COORDINATES: protein motif:HMM:NF014868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB46377.1"
                     /translation="MSGHAYATSGKLAARMGPFAGFHENREPMLRVLGMHREAAEHLD
                     SDVLPAELVEAAQRSWAVACELGNEVGVRNSQATVLAPTGTISFMMDADTTGIEPDLG
                     LVKTKKLVGGGTMKIVNQTIPRALTNLGYTEDEADEIVAYIAEHHSVLGAPHLKAEHV
                     AVFACSMGDNVVHYSGHLAMMAATQPFLSGAISKTVNTPESTTVEEIEQIYIDGWKLG
                     LKAVAVYRDNCKVGQPLSTGKAKPVGAVEAATQAVETITVVKHEPVRERLPRARTSKT
                     FEFRVADCKGFATIGEYADGRPGELFVRVSKQGSTLAGIMDAFAIAVSHGLQYGVPLS
                     AYVNAFVGMRFEPAGMTDDPDLRLASSLMDYLFRRIALDYLPYEERASIGLFSVSERT
                     QPTLPGVEEAVTETNQGHELPADPPSSQLGLSVDLRSVDETPVASADAPLCMQCGVKM
                     QRAGSCYVCQECGQTSGCS"
     gene            complement(1714..>2373)
                     /locus_tag="PLP95_11030"
     CDS             complement(1714..>2373)
                     /locus_tag="PLP95_11030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB46378.1"
                     /translation="NVALVVDDPFVEGLSAALCLRPVESPTGHWAADVVDVRRSADED
                     HLVPLLLSAAADRLVRNGVSSVAVPASPERPERAAALRRASLQLDCWMMIRRRGIVRI
                     DDEYEQGLPLPHLHGHRPLRPGRDLRTEDAGAWQTATMMPFDAAVPLEVVALLDPVIA
                     PDAAALTRLLVKLGDPARTDDDDSRIAIAVGAGEDRLDKALDAAGFRRSVEWWRLDLV
                     G"
BASE COUNT          407 a          768 c          819 g          379 t
ORIGIN      
        1 aggcggccat cgagatcgtg ttcatcgccc aggaaatcct ggtgggcaac gccgactacc
       61 caaccgaggc catcggtcgc aacactcggc gtttccgcca gctcggcatg ggctacacga
      121 atctcggcgc gctgctgatg gccctcgggt tgccctacga ctccgacggg ggggcgcgcc
      181 ttggccgctg cggtcccggc gctgatgtcg ggccacgcct atgcgacctc gggcaagctc
      241 gccgcccgca tggggccatt cgccggattc cacgagaacc gcgagcccat gcttcgggtg
      301 ctcggaatgc accgtgaggc ggccgagcac ctcgactccg acgtccttcc ggccgagttg
      361 gtcgaagccg cgcagcgttc gtgggcggtc gcatgcgagc tcggcaacga ggtcggggtg
      421 cgcaacagcc aggcgaccgt gttggccccg accggcacga tctcgttcat gatggatgcc
      481 gacaccaccg gcatcgaacc cgacctcggc ctcgtcaaga ccaagaagct cgtcggcggc
      541 ggaacgatga agatcgtcaa ccagacgatc ccgcgtgcgc tcacgaacct cggctacacc
      601 gaagacgagg ccgacgagat cgtcgcctac atcgcggagc atcactcggt gttgggtgcg
      661 ccgcacctca aggccgaaca tgtcgcggtg ttcgcctgtt cgatgggcga caacgtcgtg
      721 cactactcgg ggcacctggc gatgatggcc gcgacgcagc cgttcctgtc cggcgcgatc
      781 tccaagacgg tgaacacccc cgagtcgacc acggtggagg agatcgaaca gatctacatc
      841 gatggctgga agctcggcct caaagcggtg gcggtgtatc gcgacaactg caaggtcggc
      901 cagccgctgt cgaccggcaa ggcgaagccg gtgggcgcgg tcgaggccgc aacccaggcc
      961 gtcgagacga tcaccgtggt caaacacgaa ccggtgcgtg agcgtctgcc acgggcccgc
     1021 acctcgaaga ccttcgagtt ccgcgtggcc gactgcaagg ggttcgccac catcggcgag
     1081 tacgccgacg gccgcccggg cgagctgttc gtgagggtct cgaagcaggg gtcaaccctt
     1141 gcgggcatca tggacgcctt cgcgatcgcg gtgagccacg gcctgcaata cggggtgccg
     1201 ctgtcggcct atgtcaacgc gttcgtcggg atgcgtttcg agccggcggg catgaccgac
     1261 gatcccgacc ttcgcctggc ttcgtcgctg atggactacc tgttccggcg tatcgccctc
     1321 gattacctgc cctatgagga gcgggcatcg attgggctgt tcagcgtgtc cgagcgcacc
     1381 caacccacgc ttcccggagt ggaagaggcg gtcaccgaga cgaaccaggg ccacgagttg
     1441 ccggccgatc cgccctcgtc gcagctcggg ttgtcggtcg acctgcggtc ggtcgacgag
     1501 acgccggtcg ccagcgccga tgcgccgttg tgcatgcagt gtggtgtgaa gatgcaacgt
     1561 gcagggtcgt gctacgtctg tcaggagtgc gggcagacca gcggctgtag ctgaccgccc
     1621 actcgttctg taggtaatcg cggttgttat agccgccgca tttacctaca gaactggtgt
     1681 cacaacgaaa gacgccaagc gcctggccgc cacctagccg acgagatcga gacgccacca
     1741 ctcgacggag cggcggaatc cggcggcgtc gagcgctttg tcgagccggt cttcgccggc
     1801 gccgactgcg atggcgatgc gtgagtcgtc atcgtcggtg cgggcggggt cgccgagttt
     1861 caccaggaga cgggtcagtg cggcagcgtc gggggcgatc accggatcga gcagggccac
     1921 gacctcgagg ggaaccgcag cgtcgaaggg catcattgtg gcggtttgcc aggctccggc
     1981 gtcttcggtt cgcaggtcac gtccgggccg aaggggacga tggccgtgca ggtggggcag
     2041 tggcagcccc tgttcgtact cgtcgtcgat ccgcacgatg ccgcgacggc gaatcatcat
     2101 ccagcagtcg agttggagcg atgcccgacg aagggcagcc gcccgttcgg gccgttcggg
     2161 gctggcgggc acggccacgc tggacacccc gttgcggacc aggcggtcgg ccgccgcgct
     2221 cagcaggagt ggaacgaggt gatcctcgtc cgcggagcga cgaacgtcga ccacgtccgc
     2281 cgcccagtgg ccggtgggcg attcgaccgg gcgcaaacac agtgcggccg agagaccttc
     2341 gacaaagggg tcatcgacga cgagcgcgac gtt
//
LOCUS       DAOOMA010000451         2363 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_55959_length_2363_cov_3.023830, whole genome shotgun sequence.
ACCESSION   DAOOMA010000451 DAOOMA010000000
VERSION     DAOOMA010000451.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 2363)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2363)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2363
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55959_length_2363_cov_3.023830"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..855)
                     /locus_tag="PLP95_11035"
     CDS             complement(<1..855)
                     /locus_tag="PLP95_11035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HPB46379.1"
                     /translation="MSDLDHDLDVNAIKADFPLLTSTMAGESHDQLITYLDSAASSQK
                     PNVVIDTVSDYYRRLNANVARGVYELAEASTNAMEDARRSVARFVNAPSTNEIVFTRN
                     ATESINLVANTWGRQNLGAGDVVVLSQLEHHANIVPWQMLAAEKGFEIRWIRLDSDGL
                     LDLSDLDRLLVGAKLLSVSAMSNVLGTLTPIPQLVEAAHAAGAIANIDACQFVPHVPT
                     DVQAWGADFVSFSAHKMCGPTGVGALWGRMELLESTPPFLGGGGMILNVTQDGFSTTD
                     VPQKFEAGT"
     gene            complement(852..1622)
                     /gene="sufC"
                     /locus_tag="PLP95_11040"
     CDS             complement(852..1622)
                     /gene="sufC"
                     /locus_tag="PLP95_11040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015798015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HPB46380.1"
                     /translation="MNAPLFEISDLRAAPASVESGADILNGLSLTIRDGEVHAIMGPN
                     GSGKSTLASTLMGSPEYRVTGGTIRYKGDDITNWPVDERAKAGMFLAFQYPQEVSGVP
                     VRSFLRQAMGQRKGIEDMSILEVRVAMMEWMTRLDMDPAFADRMLNEGFSGGEKKRNE
                     ILQMAMLEPDLAILDETDSGLDIDALRVVANGVNEVRSARPQMGTLVITHYQRLLDYL
                     EPQFVHILIGGRIVESGGIELTHRLESEGFAPWLEHAS"
     gene            complement(1676..2125)
                     /locus_tag="PLP95_11045"
     CDS             complement(1676..2125)
                     /locus_tag="PLP95_11045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB46381.1"
                     /translation="MPEPAINAEQRPIDTTPVHTDDLPLTPLRDRNIEVSSWIEAPRD
                     LIDLHLTLPPEAPDPTGAGRTRQTFYKRRIGPWLLWRTGPAARDHARYWAGHKDDLSR
                     SFIFRLHPDGTGSGIGPSGRLHDRFRDWKLDLKAHPDQHPDRHPTMP"
BASE COUNT          365 a          696 c          827 g          475 t
ORIGIN      
        1 ggtgcctgcc tcaaatttct gcggcacgtc ggtggtgctg aaaccgtcct gggtgacgtt
       61 gaggatcatt ccgccgccac cgagaaacgg gggggtggac tcgaggagtt ccatccgtcc
      121 ccacagcgcg ccgaccccgg tgggcccgca catcttgtgc gccgagaacg acacgaagtc
      181 ggcgccccat gcctgcacat cggtggggac gtggggaacg aactggcatg cgtcgatgtt
      241 ggcgatcgct ccagccgcgt gggcggcttc gacgagttgc ggaatgggcg tcagggttcc
      301 gagaacgttg gacatcgcgg agacactgag caatttggcg ccgacgagga gtcggtcgag
      361 gtcgctcaag tcgagcaggc catcggagtc gaggcggatc caccggatct cgaagccctt
      421 ctcggcggcg agcatctgcc acggcacgat gttggcgtgg tgctccagtt gggacagcac
      481 cacgacgtca cctgcgccga ggttctgtcg tccccacgtg ttggcgacca gattgatcga
      541 ctcggtggcg tttcgagtga agacgatctc attcgtcgat ggtgcgttga cgaaccgtgc
      601 gacgctccgg cgggcgtcct ccatggcgtt tgtcgacgcc tcggctaact cgtacacccc
      661 gcgggcgacg tttgcgttga gccgacggta gtagtcgctg accgtgtcga tgacgacgtt
      721 cggcttttgt gaggaggccg ccgagtcgag gtaggtgatg agttggtcgt ggctctcacc
      781 cgccatggtg gaggtcaaca gcgggaagtc ggctttgatg gcgttgacat cgaggtcgtg
      841 gtcgaggtcg ctcatgacgc atgctccaac cagggggcga acccttcgga ctcgagtcgg
      901 tgggtgagtt cgatgccgcc gctctcgacg atacgtccgc cgatgagaat gtgcacgaac
      961 tgcggctcga ggtagtcgag gaggcgctgg tagtgggtga tgacgagggt tcccatctgc
     1021 ggccgggccg agcggacttc gttgaccccg ttggcgacga ctcgtagcgc gtcgatatcg
     1081 aggcccgagt cggtttcgtc gagaattgcc aggtcgggtt cgagcattgc catctgcagg
     1141 atctcgttgc gcttcttttc gccgcccgag aacccttcgt tcaacatgcg atcggcgaag
     1201 gcggggtcca tgtcgaggcg cgtcatccat tccatcatcg caacgcggac ctcgagaatc
     1261 gacatgtcct cgatgccctt gcgctggccc atcgcttggc gcaggaacga ccgcaccggc
     1321 accccagaca cttcctgcgg gtactggaac gccaagaaca tcccggcctt ggcgcgctcg
     1381 tcgacgggcc aattggtgat gtcgtcgccc ttgtagcgaa tcgttccgcc ggtgacccgg
     1441 tattccgggc ttcccatcaa ggtggacgcc aaggtcgact tcccggagcc gttcggaccc
     1501 atgatcgcgt gcacctcgcc gtcgcggatt gtgagggaga ggccgttgag gatgtcggct
     1561 cccgactcga cgcttgcggg cgcggcgcgg aggtcggaga tctcgaacag gggtgcgttc
     1621 atggacacga tcctaattcc tcctatcccg gtagtgtaaa tagctgcttg tgaggctacg
     1681 gcatggtcgg gtgacggtcg gggtgctggt cggggtgcgc cttgaggtcg agtttccagt
     1741 cccggaatcg atcgtgaagg cgcccgctgg ggccgattcc ggaaccggtg ccgtcggggt
     1801 gaagccggaa gatgaacgaa cgcgacaggt cgtctttgtg gccggcccag taacgggcgt
     1861 gatccctcgc cgcgggtccc gtcctccaca gcagccacgg accgatccgt ctcttgtaga
     1921 acgtttggcg ggtgcgaccg gctccggtcg ggtccggagc ctccggtggg agcgtgaggt
     1981 gtaggtcgat caggtcgcgg ggtgcctcga tccacgagga cacctcgatg ttgcggtcgc
     2041 gcaacggcgt cagtggcagg tcgtcggtgt ggaccggcgt ggtgtcgatg gggcgctgtt
     2101 cggcgttgat ggccggctcg ggcatctcaa caacgtaccc ctcgcctcga atctgccctt
     2161 cctcgcgtgg ccgtcctcgc gcggccgtcg tgggtcggtc gcgggtcggt cggtcgctgg
     2221 cggcccacga cggctcgctg atcgggtcgt gctagacggg cgttgtgccc tttacgtttc
     2281 tggctcatca ggcgccggtt ctgcccatca aacgtcgctg gcccgaacag gtcgatgggg
     2341 tggcgctcgt cgttggatcg atg
//
LOCUS       DAOOMA010000452         2353 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_56241_length_2353_cov_4.335509, whole genome shotgun sequence.
ACCESSION   DAOOMA010000452 DAOOMA010000000
VERSION     DAOOMA010000452.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 2353)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2353)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2353
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56241_length_2353_cov_4.335509"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..252
                     /locus_tag="PLP95_11050"
     CDS             <1..252
                     /locus_tag="PLP95_11050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB46382.1"
                     /translation="VRPGPTLTEMGWGWDQQLLDHTMREWQRWGVLRHGGFIDADDIA
                     AAIVHVATAPRGMLWSLVELNPEAPLPRRSDTSDQSETN"
     gene            284..1657
                     /locus_tag="PLP95_11055"
     CDS             284..1657
                     /locus_tag="PLP95_11055"
                     /inference="COORDINATES: protein motif:HMM:NF012296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HPB46383.1"
                     /translation="MTDTDTANEPRIPEFVSGGDDTTGHLEELRHDPIGLMARVRAEH
                     GDVGAFMLVDRPVALLSGSEANEPYFRAPESALDQAEAYPFMKPVFGEGVVFDASAER
                     RREMMHNQALRDKYMRGHAEKIHAEIDRMCERFGEAGQFEMLDWFAELTIYTSTTCLI
                     GPRFREDLTAEFAELFHDLEKGTDALAFVDPYMDIASFHRRDAAREELVALIDAILQK
                     RLAEPAPEGDDRDLVDVLLSIKNEDGSLAFSADYITGMFISMMFAGHHTTSTTLSWTL
                     IELLRNPEHLAAVRGELDELYADGRSASYQALREMPITEAAIKETLRLHPPLILLLRV
                     AKEPFEVAGYTVPEGWLVGATPAISNRIEESFPDADRFDPGRYLDGRAEDLENPWNWI
                     PFGAGRHRCVGAQFAMMQLKVILSVLLKDWDFELGAPPESYVNDHSKMVVQMKSPTTV
                     KYRRRQD"
     gene            1670..1870
                     /locus_tag="PLP95_11060"
     CDS             1670..1870
                     /locus_tag="PLP95_11060"
                     /inference="COORDINATES: protein motif:HMM:NF018595.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HPB46384.1"
                     /translation="MAFRIELDTDLCQCHGVCEGEAPDVFEVRKHELHILIPEPGEQH
                     RSAVQAAVKYCPTHALSIVDLD"
     gene            1914..>2353
                     /locus_tag="PLP95_11065"
     CDS             1914..>2353
                     /locus_tag="PLP95_11065"
                     /inference="COORDINATES: protein motif:HMM:NF012398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HPB46385.1"
                     /translation="MSFHEPKLLIDGQLVDATGAATYDNTNPATGKVIGQAPDASLAD
                     IDRAIAAARRAFDETDWSTNTELRVRCLRQLHDAYLANSEEIRAMTVAEVGCPVGLTY
                     AAQLDIPVSSIAYNADLLEGYEFDTDLGEAVPLGIKTHRTIAREA"
BASE COUNT          442 a          809 c          724 g          378 t
ORIGIN      
        1 gttcggcccg gacccaccct caccgaaatg ggttggggct gggaccaaca acttctcgac
       61 cacacgatgc gcgaatggca gcgctggggc gtgttgcgcc acggcgggtt catcgacgcc
      121 gacgacatag ccgcggccat cgtgcacgtc gccacggcgc cgcggggaat gctgtggtcg
      181 ctcgtcgaac tcaacccgga ggcgccgctg ccgcgtcgca gcgacacctc cgaccaatcg
      241 gaaacgaact gaccgcaacg gtctcagacc agggggaacc gtcatgaccg acaccgacac
      301 cgccaatgag ccacgcatcc cggagttcgt ctccggcggc gacgacacca ccggccatct
      361 cgaggagttg cgccacgacc cgatcggcct catggccaga gtccgcgccg aacacggcga
      421 cgtgggcgcc ttcatgctcg tcgatcgccc ggtggcacta ctgagcggct ccgaggcgaa
      481 cgagccctac tttcgggcac ccgaatcggc gctcgatcag gccgaggcct acccgttcat
      541 gaagccggtg ttcggagagg gcgtcgtgtt cgacgcctcg gccgagcggc gtcgggagat
      601 gatgcacaac caggcgctgc gcgacaaata catgcgcggc catgcggaga agatccacgc
      661 cgagatcgac cggatgtgcg aacgcttcgg cgaggccggc caattcgaga tgctcgactg
      721 gttcgccgaa ctcacgatct acacctcgac cacctgcctc atcggtccgc ggtttcggga
      781 ggacctgacc gctgagttcg cggagctctt ccacgatctc gaaaagggca ccgatgccct
      841 cgcgttcgtc gacccctaca tggacatcgc gtcgtttcac cgacgagatg cggcccgcga
      901 ggaactcgtg gccctgatcg atgccatcct gcaaaagcgc ctggccgagc ccgcgcccga
      961 aggcgacgac cgcgacctgg tcgacgtgtt gttgagcatc aagaacgagg acggctcgct
     1021 cgctttctcc gccgactaca tcaccggcat gttcatctcg atgatgttcg ccggccacca
     1081 caccacctcc accaccctga gctggaccct catcgagttg ctgcgcaatc ccgagcatct
     1141 cgcagcggtg cgcggcgaac tcgacgagtt gtacgcggat ggccgctcgg cctcctatca
     1201 ggcgctgcgg gaaatgccga tcaccgaagc ggcgatcaaa gagacgttgc gccttcaccc
     1261 gccactcatc ctgctgttgc gcgtggccaa ggaacccttc gaggtggccg gctacacggt
     1321 tcccgaggga tggctggtcg gtgccactcc ggcgatttcg aaccggatcg aggagtcctt
     1381 ccccgacgcc gatcgcttcg acccgggtcg ctacctcgac ggccgcgccg aggaccttga
     1441 gaacccctgg aactggatcc ccttcggcgc tggccgccac cgttgcgtcg gcgcacagtt
     1501 cgcgatgatg cagctcaagg tgattctgtc ggtgctgttg aaggactggg atttcgagtt
     1561 gggtgccccg ccggagtcct acgtcaacga ccactcgaag atggtggtgc aaatgaagtc
     1621 gccgacgacg gtgaagtacc gtcgccgcca ggactgaggg ggcccgatca tggcatttcg
     1681 tatcgagttg gacaccgacc tgtgtcagtg ccacggcgtg tgtgagggcg aagcccccga
     1741 cgtgttcgag gtgcgtaagc acgagttgca catcctgatc cccgaaccgg gcgaacagca
     1801 ccggagcgcg gtgcaggccg ccgtgaagta ctgcccgacc cacgccctgt cgatcgtcga
     1861 cctcgactga cccggtgtcg gccaacaacg cacctccgca agaaaggaac cacgtgagtt
     1921 tccacgaacc caagttgctc atcgacggac aactcgtcga cgccaccggc gcagcgacct
     1981 acgacaacac caaccccgcc accggcaagg tgatcggcca ggcccccgac gcgtcgcttg
     2041 ctgatatcga ccgagccatc gccgcggcac ggcgggcctt cgacgaaacc gactggtcga
     2101 ctaacaccga actgcgcgtg cggtgtcttc gccagcttca cgatgcgtat ctcgccaact
     2161 ccgaggagat ccgggcgatg acggtggccg aggtcgggtg cccggtcggg ctgacctatg
     2221 cggcgcagct cgacatcccc gtcagttcca tcgcctacaa cgccgacctg ttggagggct
     2281 acgagttcga caccgacctc ggcgaggcgg tgcccctcgg catcaagacc caccgaacga
     2341 tcgcccgcga ggc
//
LOCUS       DAOOMA010000453         2353 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_56249_length_2353_cov_3.187990, whole genome shotgun sequence.
ACCESSION   DAOOMA010000453 DAOOMA010000000
VERSION     DAOOMA010000453.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 2353)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2353)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2353
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56249_length_2353_cov_3.187990"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..382)
                     /locus_tag="PLP95_11070"
     CDS             complement(<1..382)
                     /locus_tag="PLP95_11070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB46386.1"
                     /translation="MTAATSVDNVAWDLEPLLKTSELEGVEAFLASATADVEALEAYR
                     GRLGELDAGELATFFDKVASFNDKLGRVGNYASLQFAVATDDPEVGALMQKVQEQGTV
                     LGSRLIFIELELAELDDDTAASFEA"
     gene            complement(393..614)
                     /locus_tag="PLP95_11075"
     CDS             complement(393..614)
                     /locus_tag="PLP95_11075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HPB46387.1"
                     /translation="MSVLSGAVLSGTVTTWDDPRGIGEITADDGTVYGFHCTAIADGS
                     RQTEVGAEVHFVVRPARLGRWEAAGITPA"
     gene            complement(617..1492)
                     /gene="hisG"
                     /locus_tag="PLP95_11080"
     CDS             complement(617..1492)
                     /gene="hisG"
                     /locus_tag="PLP95_11080"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HPB46388.1"
                     /translation="MLNLVLPKGSLEKATFQLFADADLPIHRNSAVTYTATIDDPRIA
                     TVRILRPQEIPSYVAQGLFDIGICGRDWVEETSSKVVSLGEMKYSKATSNPIRVVVAV
                     AGDSPVDRVEDLPQGVRVSTEYPEITRRFFESKGIEADIRLSYGATEAKIPDIVDCVV
                     EITETGSALRAAGLKVIDEIMQSYTEVVANPEVYEDADKRHAMEQIMTLLQGVLEART
                     KVVVKLNVAAAHLDEVISKLPAMKTPTVNELYGQSGYAVETVVEKRQINVLIPALKDA
                     GASDIIELPLSKIVH"
     gene            1549..>2353
                     /locus_tag="PLP95_11085"
     CDS             1549..>2353
                     /locus_tag="PLP95_11085"
                     /inference="COORDINATES: protein motif:HMM:NF012797.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HPB46389.1"
                     /translation="MTPIHLTNPDDPRVSDFRRLNDTAFRRSVEAPGPFHKGIFIVEG
                     WLAAQRLGRSRYGARAVLVDEARLERAAEQLGHVRAPVFAAPRDLLDEIVGFPLHRGV
                     VASADRGLAVLASTVIGRGRNLVVCEGINDAENLGSVIRNAAALGGDGLLVDTTTCDP
                     LARRTVRVSVGWALRLPFARLALADGLSALHESGVTTVALTPRRDGVDIDEVVQRELL
                     GDRVAVILGAEGHGLSEATIDAATFAVRIPMATEVDSLNVSAAAAVAMHR"
BASE COUNT          365 a          743 c          809 g          436 t
ORIGIN      
        1 cggcctcgaa ggacgctgcg gtgtcgtcgt cgagctcggc gagttcgagc tcgatgaaga
       61 tcagtcggga gccgaggacg gtgccctgct cttgaacctt ctgcatcagc gcaccgacct
      121 cggggtcgtc ggtggcgacg gcgaattgca ggctggcgta gttgccgacc cggccgagct
      181 tgtcgttgaa cgaagcgacc ttgtcgaaga aggtcgcaag ctcgccggcg tcgagttcgc
      241 cgaggcgacc ccggtaggcc tcgagcgcct ccacatcggc ggtggccgag gccaggaacg
      301 cttcgacgcc ctcgagttcg gaggttttca gcagcggttc gaggtcccag gcgacattgt
      361 caacggaggt ggcggcagtc atggggttca gcctacgcgg gggtgatccc cgcggcttcc
      421 catcggccca gacgcgcggg cctcacgacg aagtgaacct cggcgcccac ctcggtctga
      481 cgcgatccgt ccgcgatcgc cgtgcagtgg aacccgtaga ccgtgccgtc gtccgcggtg
      541 atctcgccga ttcctcgcgg gtcgtcccag gtggtgacgg ttccgctcag gacggctccg
      601 ctgaggacgc tcacgttcag tgcacgatct tcgacagcgg caattcgatg atgtcgctgg
      661 ctcctgcgtc tttcagcgcc gggatcaaca cgttgatctg acgcttctcg acgacggtct
      721 ccaccgcata tcccgactgc ccgtacagtt cgttgaccgt cggcgtcttc atggccggca
      781 gtttcgaaat gacctcgtcg aggtgggcgg cggcgacgtt gagcttcacc acgaccttgg
      841 ttcgggcctc gagcacgccc tgcagcaggg tcatgatctg ttccatggcg tgacgtttgt
      901 cggcgtcctc gtacacctcg gggttggcca cgacctcggt gtagctctgc atgatctcgt
      961 cgatcacctt caggcccgca gcgcgcagcg ctgagccggt ttcggtgatc tccacgacgc
     1021 agtcgacgat gtcggggatc ttcgcctcgg tcgccccgta gctgagccgg atgtcggcct
     1081 cgatcccctt ggattcgaag aatcggcggg tgatctcggg atactcggtg gagacccgca
     1141 ccccctgggg caggtcctcg acgcggtcga ccggcgaatc cccggcgacc gccacgacca
     1201 cccggatcgg gttggaggtg gccttggagt acttcatctc gccgagcgac acgaccttcg
     1261 aggaggtctc ctcgacccaa tcgcgaccac agatgccgat gtcgaacagc ccctgggcga
     1321 cataggacgg gatctcctgg gggcgaagga tccggacggt ggcgattcgg gggtcgtcga
     1381 tcgtggcggt gtaggtgact gccgagttgc gatggatggg aagatccgcg tcggcgaaca
     1441 actgaaacgt cgccttctcg agcgatccct tgggcagcac cagattcaac atggcgccaa
     1501 aagtagtgcc gcggcgacag ggtctcgaaa tgaatagcgt ctgaactcgt gaccccgatc
     1561 cacctgacca accccgacga cccacgggtg agcgatttcc gacggctgaa cgacacggcg
     1621 tttcggcgtt cggtcgaggc tcccggtccg ttccacaagg gaatcttcat cgtcgaaggg
     1681 tggttggccg cccagcgtct ggggcggtcg cgctacggcg cccgcgcggt cctggtggac
     1741 gaggcccgcc tcgaacgagc ggcggaacaa ctcgggcacg tgcgcgcccc ggtattcgcc
     1801 gctccccggg acctgttgga tgagatcgtc gggtttccgc tgcatcgcgg ggtggtggcg
     1861 agcgccgatc gcgggttggc ggtgctggcc tccacggtga tcggccgagg ccggaacctg
     1921 gtcgtgtgcg agggcatcaa cgatgcggag aacctcggca gcgtgattcg caatgccgca
     1981 gcgttgggtg gcgatggcct gctcgtggac accacgacct gtgatccact cgcgcgccgc
     2041 accgtgcggg tgtcggtcgg gtgggcgctg cggctcccct tcgcgcgact agcgctggcc
     2101 gacgggttgt cggctctcca cgaatctggg gtgacgaccg tggcgttgac ccctcgtcgc
     2161 gacggggtgg atatcgatga ggtggtgcag cgcgaacttc tcggggatcg cgtcgcggtg
     2221 attctggggg ccgaaggcca cggactgagc gaggccacca tcgacgccgc cacatttgcg
     2281 gtgcgtatcc cgatggcgac agaggtcgat tcgctcaatg tctcggctgc ggccgcggtc
     2341 gcgatgcacc gcc
//
LOCUS       DAOOMA010000454         2349 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_56385_length_2349_cov_4.159983, whole genome shotgun sequence.
ACCESSION   DAOOMA010000454 DAOOMA010000000
VERSION     DAOOMA010000454.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 2349)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2349)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2349
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56385_length_2349_cov_4.159983"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..289)
                     /locus_tag="PLP95_11090"
     CDS             complement(<1..289)
                     /locus_tag="PLP95_11090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB46390.1"
                     /translation="MTDIGVEFNFIPSLLGVRGRMVDSDLVLDLHPNPAILHHGIVRA
                     SVLAYVVDAVAGIAIDTDPERWSFTTDLTLRMRPDPITATEITTGTHHLLRD"
     gene            complement(417..1259)
                     /locus_tag="PLP95_11095"
     CDS             complement(417..1259)
                     /locus_tag="PLP95_11095"
                     /inference="COORDINATES: protein motif:HMM:NF019829.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PaaX family transcriptional regulator C-terminal
                     domain-containing protein"
                     /protein_id="HPB46391.1"
                     /translation="MSTASNIRSAVDSGSDAAGPSVSLQRLTARSVLLSVLLGTDGAW
                     LPTPLLLSTTALFGIADGTSRTALSRMSAAGEVEATGRGYRIVSGDLLARQHRQATGR
                     SGERRTWDGSWRQVIVGVGERRSAGDRAATRTALGTARFGELREGVWLRPDNLAPSEA
                     VEPWADSPDTLSATVARIDGDDAELATRLWDLDGWAGQAAGLIGDMTPMTRRLERGDA
                     EALAESFMINAAVLRLLAADPLLPDRLAPTRWPGHRLRDDYERFDAAFRHVITEHFAR
                     HADR"
     gene            1289..2329
                     /locus_tag="PLP95_11100"
     CDS             1289..2329
                     /locus_tag="PLP95_11100"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: protein motif:HMM:NF012699.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid desaturase"
                     /protein_id="HPB46392.1"
                     /translation="MASPSVLVRTTMVPSPDLLADVLPTERLTVSGMPVPEVRRELRR
                     IDNLRNVGSVVLCWAQAAITIGLCIWIGHPLAYLAGIVLMGPVFARFLILGHEAAHKL
                     LFTNKRANDFVGKWLLDAPGLVPFGIYRRSHFAHHKEEFGPTEPDIAFYRGYPMPASS
                     LWRKLRRDALFSSGWKNLTPLFRALKSATGRRLAMPIYVTQAVIFAVFWAIGHPWLYL
                     VLWIVPWMTSWRVINRLRAIAEHGGMEASKDRRKTTHHVRQSRLASFWVVPFNTGWHL
                     AHHVDMGVPWRNLPALHRELVDAGWVVPELEYPNYRALWKALASGAEVDAGIAGKRAT
                     ASGEGTAGSHPD"
BASE COUNT          372 a          758 c          807 g          412 t
ORIGIN      
        1 cgtcgcgcaa caggtgatgg gtcccggtgg tgatctcggt ggcggtgatc gggtcgggcc
       61 gcatgcgcag ggtgaggtcg gtggtgaagg accatcgttc ggggtcggtg tcgatcgcga
      121 tgccggcgac cgcgtcgacg acgtaggcca acacgctggc gcgcacgatg ccgtgatgga
      181 ggatcgccgg gttcggatga aggtccaaca cgagatcgct gtcgaccatg cggccccgaa
      241 caccgagcag cgacgggatg aaattgaact cgacgccgat gtcggtcatt tgggcctcct
      301 tgggtgcgtt catcctgcct gcttgcgcga acttcctgcc agaagcgaca ccacgtatcg
      361 ctgtacgcag gaagtttgtg gcgctaggtg cgcaggccgc gtcagcgcac gccccatcaa
      421 cgatcggcgt ggcgggcgaa atgctcggtg atcacgtgtc gaaacgcggc gtcgaagcgt
      481 tcgtagtcgt cgcgtaggcg atgaccgggc caccgcgttg gagcgagccg gtcgggcagc
      541 aacggatcgg cggccaacag ccgcaacacc gcggcattga tcatgaatga ctccgcgagc
      601 gcctcggcgt cgccgcgttc gagtcggcgg gtcatcggcg tcatgtcccc gatgaggccg
      661 gccgcctgtc ccgcccaacc gtcgaggtcc cacagccgcg ttgcgagttc ggcatcatcg
      721 ccgtcaatcc gagcgacggt tgcgctgagc gtgtcggggg agtcggccca cggctcgacc
      781 gcctcggagg gcgcgagatt gtcgggtcga agccagacgc cctcgcgtag ctcgccgaat
      841 cgggcggtgc ccagtgccgt gcgcgttgct gctcgatcgc ccgcacttcg gcgctcgccc
      901 accccgacga tcacctgtcg ccacgacccg tcccacgttc ggcgctcgcc gcttcgcccg
      961 gtggcctgcc ggtgctgacg ggcgagcagg tcgcccgaaa cgatgcggta gccgcggccc
     1021 gtcgcttcga cctcgccggc ggcggacatg cggctgagcg cggtgcggga ggttccgtcc
     1081 gcgatgccga acagtgccgt ggtgctcaac agcagtggcg tcgggagcca cgccccgtcg
     1141 gtgccgagca gcaccgacaa cagcaccgaa cgggcggtca gtcgctgcag cgagacgctc
     1201 ggccccgcag catccgagcc ggaatcgact gccgatcgaa tattactggc tgttgacaca
     1261 tgtttgagtt tcgtagtaaa tctcgagcat ggcaagccca tcggtactcg tgcgaaccac
     1321 gatggttccg tccccggatc ttctcgcgga cgtgttgccg accgagcgcc tcaccgtctc
     1381 gggaatgccg gtaccggagg tccgccgaga gctgcgacgt atcgacaacc tccgaaatgt
     1441 cggatccgtg gtgctgtgtt gggcgcaggc agcgatcacc atcgggttgt gcatctggat
     1501 cggccacccg ctggcctacc tcgccggcat cgtgttgatg ggcccggtgt ttgcccgctt
     1561 cctgatcctc ggtcacgagg cggcgcacaa gttgttgttc acgaacaaac gggcgaacga
     1621 cttcgtcggc aagtggctgc tcgacgcccc gggcctcgtc ccgttcggga tctaccggcg
     1681 ttcgcatttc gctcatcaca aggaggagtt cggtccgacc gagcccgaca tcgccttcta
     1741 ccggggctac ccgatgccgg cctcgtcgct gtggcgaaag ctccggcgcg acgccttgtt
     1801 cagctcgggg tggaagaacc tcacgccgct gtttcgggca ctgaagagcg cgacgggtcg
     1861 gcgtctcgcc atgcccatct acgtcaccca ggctgtgatc ttcgcggtgt tctgggcgat
     1921 cggacacccg tggttgtatc tcgtgttgtg gatcgtgccg tggatgacgt cgtggcgggt
     1981 gatcaaccgg ctgcgggcca tcgccgagca cggcgggatg gaggcgagca aggatcgccg
     2041 caagaccacc catcatgtgc ggcagtcgag gcttgcgagc ttctgggtcg tgccgttcaa
     2101 caccggctgg catctcgccc atcatgtcga catgggcgtg ccgtggcgga acctgccggc
     2161 gctgcatcgc gaactcgtcg atgccgggtg ggtcgtgccc gaactcgagt atccgaacta
     2221 ccgcgcgctg tggaaggcgc tcgcctccgg tgccgaggtc gatgccggca tcgcggggaa
     2281 acgagcgact gccagcggtg agggcaccgc gggcagccac ccggattgac gagaaccgcc
     2341 ggatcagcc
//
LOCUS       DAOOMA010000455         2349 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_56388_length_2349_cov_4.096774, whole genome shotgun sequence.
ACCESSION   DAOOMA010000455 DAOOMA010000000
VERSION     DAOOMA010000455.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 2349)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2349)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2349
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56388_length_2349_cov_4.096774"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1306)
                     /locus_tag="PLP95_11105"
     CDS             complement(<1..1306)
                     /locus_tag="PLP95_11105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020379588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HPB46393.1"
                     /translation="MSDQTIEHYLDEHRTFQPSPEFVAQANVSDRSLYDAADADYEQF
                     WADQARRFVSWYDDFHTPLEWNAPDAKWFVGGTLNVSYNCLDRHVEAGLGDRVALLWE
                     GEPGDIRAITYAELTDEVSRFANVLKDLGVRRGDRVAIYMPMIPELPVAMLACARIGA
                     AHSVIFGGFSPDSIADRVNDGECVAVVTADAGYRRGAPSALKVNVDTALAECTSVRNV
                     VVVNRCDLDVDMVEGRDHWYHDLIASASTVCEPERMDAEDLLFLLYTSGTTAKPKGIM
                     HTTGGYLTQTAFSTYYTFDLKADRDIYWCTADCGWVTGHSYIVYGPLANAATSVMYEG
                     TPDTPRLEDRQGDPSTWHKDRFWDIIERHGVTQLYTAPTAIRTFMKWGAQEPARHDLS
                     SLRLLGSVGEPINPEAWVWYHLNIGAERCPIVDTWWQTETGAH"
     gene            1404..2225
                     /locus_tag="PLP95_11110"
     CDS             1404..2225
                     /locus_tag="PLP95_11110"
                     /inference="COORDINATES: protein motif:HMM:NF013793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HPB46394.1"
                     /translation="MGEVVAVVNQKGGVGKTSVTLGLTSAAMARGHRVLVIDLDPQGA
                     SSWVLGVDTEQVQRSVADVLNANRAGSAKQAITPSTWSPLVDVLPSVPALQHLEVIRG
                     GLDTMLLGSKTETRLRKGLQGVTDAYDLVLIDCPPSLGALTTNGLAAATQTIIVVEPT
                     ALSLRGINPVADLIEEVWDRHNDRLNLAGVIVNRSPARSADADYHLEELVHIVGRKAV
                     WQPYIPLRVAIAEAAAARAPIHHMGSRAAAACEVYETLYKKLWRIVRPTKAATTA"
BASE COUNT          377 a          741 c          802 g          429 t
ORIGIN      
        1 tgtgggcgcc ggtttcggtc tgccaccacg tgtcgacgat cgggcagcgt tcggcaccga
       61 tgttgaggtg ataccagacc caggcctcag ggttgatcgg ttcacccacc gatccgagca
      121 gtcgcaggct ggacaggtcg tggcgggcgg gttcctgggc gccccacttc atgaaggtcc
      181 ggatcgcggt gggtgcggtg tagagctggg tgacgccgtg gcgttcgatg atgtcccaga
      241 agcggtcctt gtgccacgtc gacgggtcgc cctgtcggtc ctcgaggcgt ggcgtgtcgg
      301 gggttccctc atacatgacc gaggtcgcgg cgttcgcgag tgggccatag acgatgtagc
      361 tgtgcccggt cacccagccg cagtccgcgg tgcaccagta gatgtcgcgg tcggctttga
      421 ggtcgaaggt gtaataggtg ctgaacgccg tctgggtgag gtagccgccg gtggtgtgca
      481 tgatgccctt gggcttggcg gtggtgccgg aggtgtagag gagaaacagc aggtcctcgg
      541 cgtccattcg ttcgggttcg cacaccgtgg aggccgatgc gatgaggtcg tgataccagt
      601 ggtcgcggcc ctccaccatg tcgacgtcga gatcgcagcg gttcactacg accacgttgc
      661 gcacgctcgt gcattccgcc agtgcggtgt cgacgttcac cttgagcgca gagggtgcgc
      721 cgcggcgata tccggcatcg gcggtgacca cggcgacgca ttcgccgtcg ttgacgcggt
      781 ccgcgatcga gtcggggctg aacccgccga agatgaccga gtgagcggca ccgatgcggg
      841 cgcaggccag catggcgacg ggcagttcgg ggatcatcgg catgtagatc gccacgcggt
      901 cgccgcgccg cacgccgaga tctttcaaca cgttggcgaa acggctgacc tcgtcggtga
      961 gttccgcgta ggtgatggcg cggatgtctc ccggttcgcc ttcccacagc agcgcgaccc
     1021 ggtcgccgag gccggcttcg acgtggcggt cgaggcagtt gtacgaaacg ttgagggtgc
     1081 cgccgacgaa ccacttcgca tcgggggcgt tccactcgag cggcgtgtga aagtcgtcat
     1141 accagctcac gaaacgccgg gcttgatcgg cccagaactg ttcgtaatcg gcgtcggctg
     1201 cgtcgtagag cgaacggtcc gaaacgttcg cctgcgccac aaactccggt gacggttgaa
     1261 acgtgcgatg ttcatcgagg taatgctcga ttgtctggtc gctcacgacg tatccccctt
     1321 gctcgcggcc ggtcggccgc acccaaaaac tagcccgacg atcgaccttg tttcgtcgcc
     1381 cggcaccgac aacgatgtca gccatgggag aggtcgtcgc cgtcgtcaat cagaagggtg
     1441 gggtcggcaa gacctcggtc acgctcgggc tcacgtccgc cgccatggct cgtggacatc
     1501 gggtgctcgt catcgacctc gacccacagg gggcgtcgtc gtgggtgctc ggggtcgaca
     1561 ccgaacaggt tcagcgttcg gtggccgatg tgttgaacgc caaccgtgcc ggctccgcca
     1621 aacaggcgat cacgccctcc acctggagcc cactggtcga cgtgttgcca tcggtgccgg
     1681 cgctgcagca cctcgaggtg atccgcggcg ggctcgacac gatgctgctc ggatccaaga
     1741 ccgagacccg cctgcgcaag ggccttcagg gcgtgaccga cgcttacgac ctcgtgctga
     1801 tcgattgccc tccatcgctc ggtgcgctca cgaccaacgg gttggcggcc gctactcaga
     1861 cgatcatcgt ggtcgagccg accgcgctca gcctgcgcgg catcaatccg gtcgccgatc
     1921 tgatcgagga ggtgtgggat cgccacaacg accgactcaa cctcgccggg gtgatcgtga
     1981 accgctcgcc ggcacgcagc gctgatgccg actatcacct cgaggaactc gtgcacatcg
     2041 tcggccgcaa ggcggtgtgg cagccctaca tcccactgcg ggtggcgatc gccgaggccg
     2101 ctgcggctcg ggcgccgatc catcacatgg ggtcgcgggc cgcggccgcg tgcgaggtgt
     2161 acgagacgct ctacaagaag ctctggcgca tcgtccgccc gaccaaggcc gcgacgactg
     2221 cctgagcgcg cctgcgcgct gcctgacgcc tgcgcgctga cggttcgtcc gcgccgccca
     2281 cagttcgaac tttgtgcggg ctgtttcgaa ctttacgcgg aaagtgacac gtggtgtcac
     2341 tttccgcgt
//
LOCUS       DAOOMA010000456         2348 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_56432_length_2348_cov_4.020497, whole genome shotgun sequence.
ACCESSION   DAOOMA010000456 DAOOMA010000000
VERSION     DAOOMA010000456.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 2348)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2348)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2348
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56432_length_2348_cov_4.020497"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            69..848
                     /locus_tag="PLP95_11115"
     CDS             69..848
                     /locus_tag="PLP95_11115"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HPB46395.1"
                     /translation="MNDPTPDSTTDVLEADWQRRVVGRSLRSATERSVDRGYSLILAA
                     QKVIERSNGADVTVQEIADEAGQSLRTLYQYFESKDDLLLAVFEEAMRIYARMITEAV
                     ADLSTPLERLGGVLLSAVLMPEFSRPSQDRGLSRLRLKLAESEPDRVADAQRNVAAIL
                     IGAVRDAVASGDITNVDPETSTFTLMSMNTTFITAEVLGNDAGMRRPTAYANTLFCLR
                     GLGASVDEEWLSGVEARLRLPVRRSRNSKKTAPKKSAARNS"
     gene            995..1225
                     /locus_tag="PLP95_11120"
     CDS             995..1225
                     /locus_tag="PLP95_11120"
                     /inference="COORDINATES: protein motif:HMM:NF015947.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HPB46396.1"
                     /translation="MTHRVGPKGQVVIPKQIRDELGIEPGDEVSFWRDGDHVEVRPVR
                     GRRPLRGRFEGAALVDVLAEEREADRRRESTR"
     gene            1222..1611
                     /locus_tag="PLP95_11125"
     CDS             1222..1611
                     /locus_tag="PLP95_11125"
                     /inference="COORDINATES: protein motif:HMM:NF013970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HPB46397.1"
                     /translation="MNVVLDSWAVLRYLENTDPAASLVAELLEDRRPLMSWINLGEVY
                     YVVRRMFGESEAMETVRDLRARVEAVLPDESLVLAAARIKADVAMSYADAFAASLAQT
                     RAATLWTGDPELLVEGSAWQWRDLRGR"
     gene            complement(1710..>2348)
                     /locus_tag="PLP95_11130"
     CDS             complement(1710..>2348)
                     /locus_tag="PLP95_11130"
                     /inference="COORDINATES: protein motif:HMM:NF012296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HPB46398.1"
                     /translation="YFLELTNEKRANPTDDMLSKLTQVSVERSDGESTQLTDREISGF
                     ATLLGGAGAETVTKLVANAVVLFDEHPDVWQRIRNDHSLIPAAVEEILRIQPPSQYQG
                     RYSTAERTFSGGTIPAGFPVLLLTGAATRDERQFDNPDEFNIDRPPAVALGFGHGVHS
                     CLGAALARTESRIAIEELAKRWTRLEVDHDGLERVTMSNVAGFSNVPVRARS"
BASE COUNT          392 a          729 c          814 g          413 t
ORIGIN      
        1 atggcggtcg tcggccgggt gctgctcggc cacaaagccc aaaccgttgt cgacctgatg
       61 gatcacgtgt gaacgaccca accccggatt ccacaaccga cgtcttggaa gccgactggc
      121 agcgccgcgt cgtggggcgt tcgctgcgca gcgccaccga gcgctccgtc gaccgcggct
      181 acagcctgat ccttgcggcg caaaaggtga tcgagcgttc caacggcgca gatgtcacgg
      241 ttcaggagat cgcggacgaa gccggccagt cgcttcgcac cctgtatcag tacttcgaga
      301 gcaaggacga tctgttgctc gccgtgttcg aggaagcgat gcggatctac gcccggatga
      361 tcaccgaggc ggtggccgac ctctccactc cgctcgaacg gctcggcggc gtgttgttgt
      421 cggcggtgct gatgccggaa ttcagccgtc cgagccagga tcgcgggctg agccgactga
      481 gactgaagct cgccgaatcc gagccggacc gggtggccga cgctcagcga aacgtcgcgg
      541 cgatcctgat cggtgcggtt cgcgacgcgg tggcgagcgg cgacatcacc aacgtcgatc
      601 ccgagacgtc gacattcaca ctgatgtcga tgaacacgac cttcatcacc gccgaggtgc
      661 tcggcaacga cgccgggatg cgccgtccga cggcatacgc gaacacgttg ttttgcctgc
      721 ggggcctcgg cgcgagcgtg gacgaagaat ggctgagcgg cgtcgaggcg cgtctgcggt
      781 tgccggttcg ccggagtcgc aactcgaaga agacggcgcc gaagaagtcc gcggcccgca
      841 acagctgacg cgcccgccgc gcccgctccg cctgttgcgc cgtttcgcct gttctccgat
      901 aaccagtggt cgtgatcacg accactggtt atcgggaaac aggccggtgg gtgggcaggc
      961 cggtgagtgg cggcggacgg ttatactcgg cggtatgact caccgcgttg ggccgaaggg
     1021 tcaggtcgtc atcccgaagc agatccgcga cgaactcggc atcgaacccg gtgacgaggt
     1081 gagcttctgg cgagacggcg accacgtcga agtgcggccg gtgcgcggcc gtcggccgct
     1141 tcgcggtcgc ttcgaaggtg cagcgctggt cgatgtcttg gccgaggagc gcgaagctga
     1201 ccgacgacgc gagtcgacgc ggtgaacgtc gtcctcgatt cctgggcggt gttgcgatac
     1261 ctggagaaca ccgatccggc ggcgtcgctc gtggccgaac tcctggagga tcgacgaccg
     1321 ttgatgtcgt ggatcaatct gggtgaggtc tattacgtcg ttcggcgaat gttcggtgag
     1381 tcggaggcca tggagacggt gcgcgacctg cgggctcggg tcgaggccgt gctgccggat
     1441 gaatcgcttg tgctggctgc cgcccgaatc aaggccgacg tcgcaatgtc ctacgccgac
     1501 gcgttcgccg catcgctcgc tcagacccgg gctgccacat tgtggaccgg cgatcccgag
     1561 cttctcgtcg agggctccgc ttggcagtgg cgagaccttc gcggtcggta gcagtgcgtc
     1621 gctgactcgt tctccgataa ccagtggtcg tgatcacgac cactggttat cggagaacag
     1681 gccggtcggc cggtcagcgg gtcagcgggt caggagcggg cgcgaactgg gacgttcgag
     1741 aaccccgcca cgttcgacat cgtcacccgt tcgagcccgt catggtcgac ctcgaggcgg
     1801 gtccaacgct tggcgagctc ctcgatcgcg atacggcttt cggtgcgggc cagtgcggcg
     1861 cccaggcaac tgtgtacgcc gtggccgaag ccgagcgcca ccgccggcgg tctgtcgatg
     1921 ttgaactcgt cggggttgtc gaactggcgc tcgtcgcgag tggcggcacc cgtcaacaac
     1981 agcaccggaa acccggcggg aatggtgccg cccgagaacg tgcgctcggc ggtggagtag
     2041 cggccctggt actgcgacgg cggctggatg cgcaggatct cctcgaccgc tgcggggatg
     2101 agcgagtggt cgttgcggat gcgttgccac acgtcggggt gctcgtcgaa caacacgacc
     2161 gcattcgcca ccagcttcgt cacggtctct gcgccagcac cgcccaacag cgtggcgaac
     2221 cccgagatct cgcggtcggt gagttgagtc gactcgccgt cggaacgctc gaccgacacc
     2281 tgggtgagct tcgacaacat gtcgtcggtc gggttcgctc gtttctcatt ggtgagttcg
     2341 agaaagta
//
LOCUS       DAOOMA010000457         2347 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_56461_length_2347_cov_4.078970, whole genome shotgun sequence.
ACCESSION   DAOOMA010000457 DAOOMA010000000
VERSION     DAOOMA010000457.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 2347)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2347)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2347
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56461_length_2347_cov_4.078970"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..970)
                     /gene="rsgA"
                     /locus_tag="PLP95_11135"
     CDS             complement(<1..970)
                     /gene="rsgA"
                     /locus_tag="PLP95_11135"
                     /inference="COORDINATES: protein motif:HMM:TIGR00157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HPB46399.1"
                     /translation="MTAADRLVSIGWNDDVADRISTLPTRSTTDNTLTAGRIVRVDRG
                     EVDVALGSHPDDVVRATTTTAAKDCVAGDWVVVDLEQARVEAVAARLTAFVRRAARGA
                     FAPQTLAANMDVVLVVQALDPGLNLRRLERELVLAHQSGATPVVVITKTDLVDADTVA
                     EALDQARRTAANVEVVAVSNRTGEGFDCLHQIVGPGRTFALLGSSGVGKSTLVNTFAG
                     EEVQLEGEIREGDGKGRHTTTAARLIMLSDGRLLLDTPGVRALALWESWDGLALTFPE
                     IEDLTSECGFADCFHDDEPNCAVVEAGTIDADRLDSWRRMRDEMAEL"
     gene            1158..>2347
                     /locus_tag="PLP95_11140"
     CDS             1158..>2347
                     /locus_tag="PLP95_11140"
                     /inference="COORDINATES: protein motif:HMM:NF014587.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ppx/GppA phosphatase family protein"
                     /protein_id="HPB46400.1"
                     /translation="MIDSTHPSLAAATASGRVVIPEVLAAIDIGTNSIHMIVARALSD
                     GRFEVVTRLKEMVRLGETGDDHLKELTPAAIERGVAALARCRVVLDTYGAPYAAVATS
                     AVREADNRREFIKRARREAGIDVEVVAGHEEARLIHLGVLQALPVYDRALLLCDIGGG
                     STELLLGLRGEVRASRSFKLGAIRTTQRFFADGTVTKKSVKQAREFVRSTIAPFGREV
                     RRYGFEIAVASSGTIESLTSMALAAAGQEFQSLNGAVLTREALGDVIASLLEASTPEE
                     RALIPGIDRSRSDIIVGGAIILEQVMGQFGIESVTVSDYALREGALFDLASRFSDASL
                     DHLSDIRRRSIAHLMEVGDEDPEHSVAVSRLALSMFDQLEGLHGFGHEEREVLEAASL
                     LANVG"
BASE COUNT          368 a          756 c          822 g          401 t
ORIGIN      
        1 cgagttcggc catctcgtcg cgcatgcgac gccacgagtc caaccggtcg gcatcgatcg
       61 tgcccgcctc gaccaccgcg cagttcggtt cgtcgtcgtg gaagcagtcg gcgaatccgc
      121 actccgaggt caggtcctcg atctcgggga acgtgagcgc cagcccgtcc cacgactccc
      181 agagggcgag ggcgcgcacg ccgggggtgt cgaggaggag ccgaccgtcg ctcaacatga
      241 tgagcctcgc ggcggtggtc gtgtggcggc ctttgccgtc gccctcgcgg atctccccct
      301 cgagttggac ctcctcgccg gcaaacgtgt tcaccagcgt cgacttgccg accccggaac
      361 tgccgagcag cgcgaaggtt cgccccgggc caacgatctg atgaaggcaa tcgaaccctt
      421 ctccggtgcg attcgacacg gccacgacct cgacgttcgc ggcggtgcgt cgcgcctggt
      481 ccagcgcttc ggcgaccgtg tcggcatcga ccaggtcggt cttggtgatc accacgaccg
      541 gggttgctcc gctctggtgg gcgaggacca gttcgcgctc gaggcggcga aggttcagcc
      601 ccgggtcgag ggcctgtacg accaacacca cgtccatgtt ggcggcgagg gtctgcgggg
      661 cgaacgcgcc gcgagcggcg cgccgcacga acgcggtgag gcgcgcagcg acggcttcaa
      721 cccgggcctg ttcgaggtcg acgacgaccc agtctccggc cacgcaatcc ttcgccgcgg
      781 tggtcgtcgt tgcccgaacg acatcatcgg ggtgcgaccc gagcgccacg tctacctcgc
      841 cgcggtcgac gcgaacgatc cgcccagccg taagtgtgtt gtcggtcgtg gagcgggtcg
      901 gcaacgtcga gattcgatcg gcgacgtcgt cattccagcc gatgctgacg aggcgatcgg
      961 cggcggtcat cgacggcggg gggtggcagg ggtcggcaac gccatggcct ttccaagcta
     1021 accgcccctc ccacgcctgt tctccgataa ccagtggtcg tgatcacgac cactggttat
     1081 cggagaacga gcgggagggc gtgggttggt cgggcgtagg ttgggggcgt gagtgaggtg
     1141 cgtcgtaggc tcatcgcgtg atcgattcga cacatccgtc gctcgctgcg gcgacagcga
     1201 gcggccgggt ggtgattccc gaggtgttgg cggcgatcga catcggcacc aactccatcc
     1261 acatgatcgt cgccagggcg ctgtccgacg gccgattcga ggtggtcacc cgtctcaagg
     1321 agatggtgcg gctcggcgag acgggcgacg accatctgaa ggagttgacg ccggcggcga
     1381 tcgaacgcgg cgtcgcggcg ttggcgcggt gccgcgtcgt gctcgacacc tatggcgccc
     1441 cctacgccgc ggttgccacc agtgcggtgc gagaggccga caaccgccgc gagttcatca
     1501 agcgggcgcg ccgggaggcg ggcatcgacg tcgaagtcgt cgccggccac gaggaggcgc
     1561 gcctcatcca tctcggcgtg ttgcaggcgc tgcccgttta tgaccgcgcg ttgctgctgt
     1621 gcgacatcgg aggtggcagc accgaattgt tgctcggcct gcgtggcgag gtccgggcat
     1681 cgcgcagctt caagctgggc gcgattcgca cgacccagcg cttcttcgca gacggaacgg
     1741 tcacgaagaa gtcggtgaag caggcgcgtg aattcgttcg ttcgacgatc gccccgttcg
     1801 gtcgcgaggt ccgcaggtac ggtttcgaga tcgctgtggc ctcctcggga acgatcgaga
     1861 gtctcacgtc aatggcgctc gctgccgcgg gtcaggaatt ccagagcctg aacggagcgg
     1921 tcttgacccg cgaggcgctc ggggacgtga tcgcctccct actcgaagcg tcgacgccgg
     1981 aggaacgcgc actgatcccc ggaatcgacc ggagccgctc cgacatcatc gtcgggggtg
     2041 cgatcatcct cgaacaggtg atgggccaat tcgggatcga gtcggtgacc gttagcgatt
     2101 acgcgcttcg cgagggcgcg ctgttcgacc tggcgagtcg gttcagcgat gcgtccctcg
     2161 atcacctcag cgacatccgc cgccgcagca tcgcccacct catggaggtc ggcgatgagg
     2221 accccgaaca ctccgtggcg gtgtcgcgcc ttgcgttgtc gatgttcgac caactcgaag
     2281 ggttgcacgg tttcggccac gaagagcgtg aggtgctcga ggcggcctcg ctgctggcga
     2341 atgtcgg
//
LOCUS       DAOOMA010000458         2346 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_56498_length_2346_cov_3.371454, whole genome shotgun sequence.
ACCESSION   DAOOMA010000458 DAOOMA010000000
VERSION     DAOOMA010000458.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 2346)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2346)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2346
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56498_length_2346_cov_3.371454"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..322
                     /locus_tag="PLP95_11145"
     CDS             <1..322
                     /locus_tag="PLP95_11145"
                     /inference="COORDINATES: protein motif:HMM:NF012656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HPB46401.1"
                     /translation="IQQMIADNEIDIAATRAITKMASEVIDTGAKGSAETSMAKTFSA
                     EAINRVVDRSLQICGSLGVSRDVLLSRYYQEVRPFRIYDGPSETHRWSIAKRAFRAAE
                     ARRS"
     gene            369..773
                     /locus_tag="PLP95_11150"
     CDS             369..773
                     /locus_tag="PLP95_11150"
                     /inference="COORDINATES: protein motif:HMM:NF021510.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2237 domain-containing protein"
                     /protein_id="HPB46402.1"
                     /translation="MEVERPQERNVMGGVLEPCGTDPVTGFYRDGCCSTGPEDLGSHT
                     LCAVMTEEFLMHQRSIGNDLSTPMPQYRFPGLKPGDRWCVTARNWLRAHLDGKAAPIV
                     LASTHERALDIVPLEVLVTYRIDVPDTLEGFD"
     gene            complement(808..1986)
                     /locus_tag="PLP95_11155"
     CDS             complement(808..1986)
                     /locus_tag="PLP95_11155"
                     /inference="COORDINATES: protein motif:HMM:NF024241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HPB46403.1"
                     /translation="MDTEQRTTLWIVLAVTVAMQAGNGTIFGLIAEIQDTHGISTGQL
                     GLISSALFATSLFSALGLAHYADKGHARRMMIVGLAIGAISTAWFGVATELWQFIAAR
                     GLCGLGVGMFLAAGRSSVARLSTTRAGQNLGMMVAAETTGFVFGPTIAVLLFKLGSLS
                     TPFFVLAAIQGLALVFLLTRHADVEAKTRPTPLPMWKLSGLDLLSRPKVLGAALLGLA
                     MYLPVGVYEALWSRYLTDLGASTTFVGASLTLYGVPLALLAGFGGRLVDRDGAIPVAK
                     RTLAMLVPIVILYGLVGSYWAVALVAMVESVVQATANPSAQKAMVQACPPDRIGNGQG
                     LTSAFGLAGAGLLSSVAPWVYAELGSGWLFGIVGVMCAVLATIAFSLYSRGESLVGVA
                     "
     gene            complement(2091..>2346)
                     /locus_tag="PLP95_11160"
     CDS             complement(2091..>2346)
                     /locus_tag="PLP95_11160"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012231547.1"
                     /note="Catalyzes the synthesis of acetoacetyl coenzyme A
                     from two molecules of acetyl coenzyme A. It can also act
                     as a thiolase, catalyzing the reverse reaction and
                     generating two-carbon units from the four-carbon product
                     of fatty acid oxidation; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HPB46404.1"
                     /translation="IDLVEINEAFAAQVVPSAKHLGISWDKLNTRGGAIAIGHPFGMT
                     GARIMGTLIHNLQVSDKQFGMESMCVGGGQGMAMIVERLS"
BASE COUNT          455 a          738 c          813 g          340 t
ORIGIN      
        1 gattcagcag atgatcgccg acaacgagat cgacatcgcc gccacccggg cgatcacgaa
       61 aatggcgagc gaggtgatcg ataccggggc gaagggctcg gccgagacgt cgatggccaa
      121 gacgttcagc gcggaagcga tcaatcgggt ggtcgatcga agcctgcaga tctgtgggtc
      181 cctcggggtg tcgcgggacg tcttgttgag ccgctactac caggaggtgc ggccgtttcg
      241 aatctacgac ggaccttcgg aaacccaccg gtggtcgatc gccaagcggg cgtttcgcgc
      301 cgctgaggcg cgacgatcgt agagctgcgc cgccggaacc aacgcagcga acaagacaag
      361 gagcgaacgt ggaagtcgaa cggcctcagg agcgcaacgt gatgggcgga gtgctcgaac
      421 cgtgcggaac cgaccccgtc acggggtttt accgcgacgg atgttgcagc accgggcccg
      481 aggatctggg aagccacacg ctgtgcgcgg tgatgaccga ggagtttctg atgcaccagc
      541 gaagcatcgg caacgacctg tcgaccccga tgccgcagta tcgctttccc gggctcaagc
      601 ccggcgatcg ctggtgcgtg accgcgcgca actggttgcg agcgcatctc gacggcaaag
      661 ccgctccgat cgtgttggct tccacccacg aacgagcgct cgacatcgtg ccgctcgagg
      721 tgctggtgac gtatcgaatc gatgtgcccg acaccctcga agggttcgac tgacggcgcg
      781 gttcctgagc cctcgaccct acggctgtca ggcgacgcca acgagcgatt cgccgcgtga
      841 atacaacgag aacgcgatgg tggccagaac tgcgcacatg acgccgacga tgccgaacag
      901 ccagccggac ccaagctcgg cgtagaccca cggtgcaacc gaactgagca gtcccgctcc
      961 ggcgagaccg aatgcggagg tcaggccttg accgttgccg atccgatcgg gaggacacgc
     1021 ctgcaccatc gccttctgcg ccgaggggtt ggccgtggcc tgcactaccg attcgaccat
     1081 ggcgacgagg gcgaccgccc agtacgaccc gacgaggccg tacaggatca cgatcggcac
     1141 gagcatcgcg agggtccgct tcgcgacggg gatcgccccg tcgcggtcga cgaggcgacc
     1201 gccgaacccc gcgaggagcg cgagcggcac gccgtagagc gtcagcgatg ccccgacgaa
     1261 ggtggtcgac gcccccaggt cggtgagata gcgcgaccaa agggcctcgt acacgccgac
     1321 ggggagatac atggcgaggc ccagcagcgc ggcgccgagc accttggggc gagacagcag
     1381 atcgaggccg ctgagcttcc acattggcaa cggggtagga cgggttttcg cctcaacgtc
     1441 ggcgtgccgg gtcaagagaa acaccagcgc aagaccttgt atcgccgcga gtacgaagaa
     1501 gggcgtcgag aggctgccca gcttgaacaa caacacggcg atggtcgggc cgaagacgaa
     1561 cccggtcgtc tccgccgcca ccatcatgcc gaggttctgc ccggcgcggg tggtcgacag
     1621 ccgggcgaca ctcgaccggc cggcggccag gaacatgccg acgccgagtc cgcagagccc
     1681 gcgggcggcg atgaactgcc acagctcggt ggcgacgccg aaccaggcgg tcgatatcgc
     1741 gccgatcgcg aggcccacga tcatcatccg gcgcgcgtgg cctttgtcgg cgtaatgggc
     1801 gaggccgagc gcactgaaca gcgaggtggc gaacagcgcc gatgagatca ggccgagttg
     1861 accggtcgag atgccgtggg tgtcctggat ctcggcgatg agcccgaaga tcgttccatt
     1921 gccggcctgc atggccacgg tcacggcgag cacgatccac agggttgttc gttgctcggt
     1981 gtccacggcg ggcaacgcta ccccgagacg cgaggcggcc ctcccgaatt acccgggagg
     2041 gccgcctcag atgggcagcg aggccgaggc gtcgctgtgc tacggctgta tcaggagagg
     2101 cgctcgacga tcatggccat gccttgaccg ccaccgacac acatcgactc catgccgaac
     2161 tgcttgtcgg acacctgaag gttgtggatg agggtgccca tgatgcgggc gccggtcatg
     2221 ccgaaggggt ggccgatggc gatggcgccg ccgcgggtgt tgagcttgtc ccagctgatg
     2281 ccgaggtgct tcgcggaggg aaccacctgt gcggcgaacg cctcgttgat ctcgacgagg
     2341 tcgatg
//
LOCUS       DAOOMA010000459         2335 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_56879_length_2335_cov_2.927193, whole genome shotgun sequence.
ACCESSION   DAOOMA010000459 DAOOMA010000000
VERSION     DAOOMA010000459.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 2335)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2335)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2335
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56879_length_2335_cov_2.927193"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..591
                     /locus_tag="PLP95_11165"
     CDS             <1..591
                     /locus_tag="PLP95_11165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB46405.1"
                     /translation="PYDPNNIDLSGIDGLSDEQVERAETLVSNTVQKLPKYADQATAL
                     ADGFVTIGDAGTGFEHLINWGYINDDRVLDPDYPESLVYQADPAGGRTLVSAMFMLWE
                     GATFDDVPDIAGNLTQWHIHDDLCFSDDPTAPRVAGVTSIGGTCTPPLVKFNPTPMIH
                     VWITPHPCGPFAALEGVAGGQVPEGETKACLSHHSH"
     gene            complement(705..1625)
                     /locus_tag="PLP95_11170"
     CDS             complement(705..1625)
                     /locus_tag="PLP95_11170"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HPB46406.1"
                     /translation="MFGIHEGPEKGWTAPITLIPLVVGAVAAVAFVLYERQHENPLLK
                     VEVFKNRMLSAGTGLLFLIFIVMMGMFLVITQFLMGVLGWGAVKATAGMLPMAFAMMP
                     LSAVAPLIAKKTGTRALFMIGFSMAAGGLALVASMVSVDGGYLSILPGLMVVGAGIGL
                     SMTPGTAAITGSLPPEDQGVASALNDSVREIGASVGIALMGSVLSAAYTSGVGDAASQ
                     LPGEVAHHVEEGIFGARAVSEQMIAGANGDPQMAGLGEQLLHTAQQAFVDGWSKTMWI
                     SAGIAAVAAIGAAVLAPTKSREAEFRVDLD"
     gene            complement(1670..2215)
                     /locus_tag="PLP95_11175"
                     /pseudo
     CDS             complement(1670..2215)
                     /locus_tag="PLP95_11175"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
BASE COUNT          451 a          798 c          732 g          354 t
ORIGIN      
        1 ccctatgacc ccaacaacat cgatttgtcc gggatcgacg ggctctccga cgagcaggtc
       61 gaacgcgccg agacgctcgt gagcaacacc gtccagaaac tgcccaagta cgccgaccag
      121 gcgaccgcgc tcgccgatgg gttcgtgacg atcggcgatg ccggcaccgg gttcgagcac
      181 ctcatcaact ggggttacat caacgacgac cgcgtcctcg acccggacta ccccgaatcg
      241 ctcgtctacc aggccgaccc ggcgggcggg cgcacgctgg tgtcggcgat gttcatgttg
      301 tgggagggtg cgaccttcga cgacgttccc gacatcgccg gcaatctcac ccagtggcac
      361 attcacgacg acctgtgctt ctccgacgac ccgacagccc cgagagtcgc cggcgtcacc
      421 agcatcggcg gaacgtgcac acccccgctg gtcaagttca atcccacccc gatgatccac
      481 gtctggatca ctccgcaccc gtgtgggcca ttcgccgcgc tcgaaggcgt cgccggcggt
      541 caggtgccag agggcgagac caaggcctgc ctgtcacatc acagccatta ggccgaggcc
      601 ggtcgagacc gcccaaaacg caggcttgct gtcgatggcg acgagtgagg tcgcctccga
      661 cagcaagcct gatcagtcag tcgttcgatc ggtcagcggc tcgatcagtc gaggtcgacg
      721 cggaattcgg cctcgcgact cttggtcggg gccagtacgg ccgccccgat cgccgccacc
      781 gcagcgatcc cggcggaaat ccacatggtc ttggaccagc cgtcgacaaa cgcctgttgc
      841 gcggtgtgca gcaactgctc gcctagcccc gccatctgcg ggtctccgtt cgctccggcg
      901 atcatctgct ccgacaccgc acgcgctccg aagatgccct cttccacatg gtgcgccact
      961 tcaccgggaa gctgcgaggc agcgtctccc acgccggagg tgtaggccgc cgacagcacc
     1021 gagcccatca gggcgatgcc gaccgacgca ccgatctcgc gcaccgagtc gttgagcgcc
     1081 gaggcgacac cctggtcctc gggcggcaac gagccggtga tcgcggcggt gcccggggtc
     1141 atcgacaagc cgataccggc gccgaccacc atcagcccgg gcaggatcga cagatacccg
     1201 ccgtcgaccg acaccatcga ggcgacgagt gcgagcccgc cggcggccat cgagaacccg
     1261 atcatgaaca gcgcgcgggt ccccgtcttc ttggcgatca gcggagcgac tgcggacagc
     1321 ggcatcatcg cgaacgccat cggcaacatg ccggcggtcg ccttcaccgc gccccatccg
     1381 agcacgccca tgaggaactg ggtgatgacg aggaacatgc ccatcatgac gatgaagatc
     1441 agaaacagca gcccggttcc cgccgacaac atgcggttct tgaacacctc gaccttcaac
     1501 agcgggttct cgtgttggcg ctcgtagagc acgaaggcca ccgctgcgac ggcgccgacc
     1561 accagtggga tgagggtgat cggggcggtc cagcccttct cggggccctc gtggattccg
     1621 aacaccaggc cgccgacggc gaccgccgac agcagcgaac cgacatagtc gaacgaccgc
     1681 catggtggtc ggcaccgtca ggtaccaggc gggccgacaa tgcgaatgca atgaccgcta
     1741 gtgcgatcgg catgatgaac acccatcgcc atgatgcgtt gtcgacgatg agcgagctga
     1801 tgaacatgcc gaagatggcg ccggcaccgg cgaatcccga ccagatgccg atggcgcgct
     1861 gacgctcatc gccgtggaag gttgaggtga tgaccgacag ggtgaccggc atgatcatcg
     1921 cagcgccgac accggcgatg gcccgggcgc cgatgagcat ggcgaccgac ccggcaaagg
     1981 cgccgacgac gttcgacacc aggtagacgg tgaggcccaa caggagaacc tttcggcggc
     2041 tccaccggtc gccgatcgcg cccaccggca tcaacagggc ggccagtgcc agcgtgtagg
     2101 cgttggcgat ccacaagaag tccccggttg aggctccgag gctttcggag atcttcatct
     2161 gcgccgagtt gaggcctgac accgcagcca tgaccgcgat gagcgcaaca cacatcgctg
     2221 tgaggatcag gttcttttgc cgagagctca gctcgggctc gtcatggtgg acgaccagat
     2281 ggtcgtcccc ggtgagtact gcggtcatct cgtctcggct cgattcgtcg gtgag
//
LOCUS       DAOOMA010000460         2334 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_56912_length_2334_cov_3.223782, whole genome shotgun sequence.
ACCESSION   DAOOMA010000460 DAOOMA010000000
VERSION     DAOOMA010000460.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 2334)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2334)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2334
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56912_length_2334_cov_3.223782"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..193
                     /locus_tag="PLP95_11180"
     CDS             <1..193
                     /locus_tag="PLP95_11180"
                     /inference="COORDINATES: protein motif:HMM:NF014638.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006109 - regulation of carbohydrate
                     metabolic process [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="carbon storage regulator"
                     /protein_id="HPB46407.1"
                     /translation="IIVNGNITITVLEIRGDHIRIGIDAPREISVHREEIHAELARAN
                     KAATGSRAADVARLPRPGV"
     gene            complement(218..1261)
                     /locus_tag="PLP95_11185"
     CDS             complement(218..1261)
                     /locus_tag="PLP95_11185"
                     /inference="COORDINATES: protein motif:HMM:NF012784.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HPB46408.1"
                     /translation="MRTAAPGSTVGRVIEVIVTAVHDAEIEVKLADGRIGVIARSEYR
                     DRPAPTVGSTIDAAVLAREDPRERVALSNSWARKLRHWERVEAAKASGEPLTGTVERT
                     IKGGLLVDLGLRAFLPSSMIGEHGPQDPEAMEPEKLVGTEVTVIVTELDRALDRVVVS
                     RRDHLRRQRRQVERDVFSRVKVGDVVTGRITQLLDYGAYIDIDGVRALLHRSEVSWGR
                     VSKVSQYLSVGDEVETEVIEVQKSKRRIGLSLRRRQPDPYASLEIGSVHHATVTRLVD
                     YGVFAQLNDGEIVGLVHLSELSELPGYRPDELVTPGEAIVVKVLSVDLDKRRIALSVR
                     QATVATPPPATPG"
     gene            1512..>2334
                     /locus_tag="PLP95_11190"
     CDS             1512..>2334
                     /locus_tag="PLP95_11190"
                     /inference="COORDINATES: protein motif:HMM:NF024777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LamG domain-containing protein"
                     /protein_id="HPB46409.1"
                     /translation="MELSSAGGYEQVVHEDHPSSYWRLGDGTTADAADASTAGTSVGK
                     NRAGQSTPTVSGAIAGDPNGATQFNASTFDVASGLVSGSSSIELWFKTANQGGLIMAM
                     KPDASQFVPLAYVGTDNKLHAGFWPMPQVVSPATVTDNQWHHLVISSASTSVTVFLDA
                     VPIGMQHGAPNMLEMTATYFGTSLETGWPYSPGGWRPLNGALDEIAIYPGALSGVQVK
                     THFDAARIGVVDAQPHRIRVDFQDKAGTAALSLRQGTPGATAALASSQLSPRYNLV"
BASE COUNT          382 a          718 c          808 g          426 t
ORIGIN      
        1 gatcatcgtc aacggcaaca tcacgatcac ggtgttggag atccgcggcg accacatacg
       61 catcgggatt gatgcccctc gggagatctc ggttcatcgc gaagagatcc acgccgagtt
      121 ggcccgcgcc aacaaggcag cgaccggctc gcgcgccgcc gacgtcgccc gtcttccccg
      181 gccaggagtc tgactcgcac ggcccccgat ccggcgatta tcccggtgtc gctggcggag
      241 gtgtggcgac cgtcgcctgg cgaaccgaca gtgcgatgcg gcgcttgtcg agatcgaccg
      301 acagcacttt gacgacgatg gcctcgccgg gggtgaccag ctcgtcgggt cggtaccccg
      361 ggagttcgga cagctcggac aggtgcacga ggccgacaat ctcgccgtcg ttgagttggg
      421 cgaacacccc gtagtcgacg aggcgggtga cggtggcgtg gtgcaccgag ccgatctcga
      481 gcgaggcata cgggtcgggt tggcgtcgtc gcagcgacaa cccgatgcgg cgcttcgatt
      541 tctgcacctc gatgacctcg gtctcgacct cgtcgccgac gctgaggtac tgggacacct
      601 ttgagacgcg accccatgac acttcgctgc gatgcagaag ggcgcggacg ccgtcgatgt
      661 cgatgtatgc gccgtagtcg aggagctggg tgatgcgtcc ggtgacgaca tcgccgacct
      721 tcacccgcga aaacacatcg cgttcgacct ggcgtcgttg gcggcgcagg tggtcgcgtc
      781 gcgagaccac gacccggtcg agtgcacggt cgagctcagt gacgatcacc gtgacctcgg
      841 tgccgacgag cttttcgggc tccattgcct cgggatcctg ggggccgtgc tcgccgatca
      901 tcgagctggg aaggaacgcc cgaagtccga ggtccacgag aagtccgccc ttgatggtgc
      961 gttcgacagt gcccgtgagc ggctcgcccg acgccttggc agcctcaacg cgttcccagt
     1021 ggcgcaactt gcgcgcccac gaattgctca gcgcgacgcg ttctcgcgga tcttcgcggg
     1081 cgaggactgc ggcgtcgatc gtcgaaccca cggtgggagc ggggcggtcg cgatactcgc
     1141 tgcgggcgat gacgccgatg cggccatcgg ccaacttcac ttcgatctcc gcgtcgtgca
     1201 cggcggtgac gatcacctcg atgacccggc cgactgttga gcccggtgca gcggttcgca
     1261 caccggcagt cggcggcgcc ggcgggtgct gcggtgttga ccacgacgag gagggcgaag
     1321 tcggcacgta gtcagtttgc ctgagagcca ccgaaaatgc gccggattct ctctccgggt
     1381 acgcgagcac gtaccgactg cggaatttct ttcgtcttgg ctcaagcggg tgggattggt
     1441 gccgaacggg ttggggtgcc cgtgttggcg cccactaccg aggcgctgtc aacgtcactg
     1501 caggtgctcc gttggagttg tcgagcgcgg gcggctacga gcaggtcgtg catgaggatc
     1561 atccgtcgtc gtattggcgt ttgggcgacg gcacgaccgc ggacgcggcc gacgcctcga
     1621 ctgcgggaac ctcggtaggt aagaaccggg ccggtcagtc gactccgacg gtttcggggg
     1681 cgattgccgg tgacccgaat ggggcgacgc agttcaacgc gtcgacgttc gatgtggcgt
     1741 cggggttggt gtctgggtcg tcgagtatcg agctgtggtt caagaccgcg aatcagggcg
     1801 gcctgatcat ggcgatgaaa cccgatgctt cgcagttcgt gccgttggcc tatgtcggaa
     1861 ccgacaacaa gttgcatgcc gggttctggc cgatgcccca ggtcgtctca ccggcaacgg
     1921 tgaccgacaa ccagtggcat cacctcgtga tctcctcggc gtcgacgtcg gtcacggtgt
     1981 tcctcgacgc cgtgccgatc ggaatgcaac acggcgcgcc gaacatgttg gagatgaccg
     2041 caacctattt cggtacgtcg ttggagacgg ggtggcccta cagccctggt ggttggcgcc
     2101 cgttgaacgg cgcgttggac gagatcgcga tctatccggg ggctctgagt ggcgtgcagg
     2161 tcaagacgca cttcgacgca gcccggatcg gtgttgtcga cgcccagccg caccggatcc
     2221 gagtggactt ccaagacaag gcgggtacgg ccgcgttgtc gcttcgccag ggcacacctg
     2281 gcgcgactgc ggcgttggcg agttctcagt tgagcccgcg ttacaacctg gtga
//
LOCUS       DAOOMA010000461         2329 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_57070_length_2329_cov_3.555849, whole genome shotgun sequence.
ACCESSION   DAOOMA010000461 DAOOMA010000000
VERSION     DAOOMA010000461.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 2329)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2329)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2329
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57070_length_2329_cov_3.555849"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>462
                     /locus_tag="PLP95_11195"
     CDS             <1..>462
                     /locus_tag="PLP95_11195"
                     /inference="COORDINATES: protein motif:HMM:NF019474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta hydrolase fold domain-containing
                     protein"
                     /protein_id="HPB46410.1"
                     /translation="AGDLDVYDFSARRTAAATGMVVVSVDYRRSPETPFPGGLTDTYH
                     VVSQLEEVLAGVAHTGRVHAVADSGGAAKVASIAMRVAAGEWASPIERQVLLYPSLDY
                     TLSGASVRQFAEGYFLESDRVEWYFDNYLPPGTDRAAVSPLFGPVCADHPDT"
     assembly_gap    463..472
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <473..699
                     /locus_tag="PLP95_11200"
     CDS             <473..699
                     /locus_tag="PLP95_11200"
                     /inference="COORDINATES: protein motif:HMM:NF019474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta hydrolase fold domain-containing
                     protein"
                     /protein_id="HPB46411.1"
                     /translation="LVIAAEYDPLLSEAERYVARLIEAGARAQLIVAPGMIHAYLFFE
                     HLVPDEVATTYEVIARFLRTGEATWDPSSQ"
     gene            798..1844
                     /locus_tag="PLP95_11205"
     CDS             798..1844
                     /locus_tag="PLP95_11205"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HPB46412.1"
                     /translation="MSELLAGLKIDHVATPRLRTAVLVDERHDGEPVVFVHGNVSSSL
                     FWQESILALPDGFRGIAPDLRGFGESETAPVDATRGLGDFADDLYSLLDTLDIDRAHF
                     VGWSMGGGIVMRILLERPEMVASLTLVSPVSPYGFGGTKGASGEPVTADGAGAGGGSA
                     NPDFVAALAAGDRGDDSPTSPRNVLNGAYFANEFRSPLEETFLDSMLTTKVGDDNYPG
                     DATGSENWPGFAPGTRGVLNTMVPGVHDVSGIVGVSPKPPILWVHGSKDVIVSDASMF
                     DLATLGRMDVVPGWPGEDACPPQPMVTQTRAVLDDYAAAGGSYREVELDCGHSAMVER
                     PDEFQAALAQVLRG"
     gene            complement(1942..>2329)
                     /locus_tag="PLP95_11210"
     CDS             complement(1942..>2329)
                     /locus_tag="PLP95_11210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB46413.1"
                     /translation="AGPVPSETGPPGVIAHAQRSVSGADKAAAELKDQIVITLGKVTD
                     VGSKAGSMLQAKMELRALRSADPATLSGQITRSIELLIALGRNVRQLDGTGSVDGWGQ
                     VEMASFVDHQRSILESNGWSDPFGTT"
BASE COUNT          368 a          789 c          757 g          405 t
ORIGIN      
        1 ggccggcgac ctcgacgtct acgacttctc ggcccgacgc accgccgcgg caaccggcat
       61 ggtcgtcgtg tcggtggact atcgacgctc acccgagacc ccgtttcccg gcggcctcac
      121 cgacacctac cacgtggttt cgcagctcga ggaggtgctg gcgggcgtgg cccacaccgg
      181 ccgggtccat gcggtggccg attcgggcgg cgccgccaag gtcgcatcga ttgcgatgcg
      241 cgtcgccgca ggggagtggg cctcgccgat cgaacgccaa gtgttgctgt atccgagtct
      301 cgactacacg ctgagcggtg cgtcggttcg ccaattcgcc gagggctact tcctcgaatc
      361 cgatcgggtc gagtggtact tcgacaacta cctgccgccc ggaaccgacc gcgccgcggt
      421 gtcgccgctg ttcgggcccg tttgcgccga tcatcccgac acnnnnnnnn nnccctcgtg
      481 atcgcggccg aatacgaccc gctgctctcc gaagccgagc gatatgtcgc ccgcttgatc
      541 gaggccggcg cacgggccca gctgatcgtc gcccccggaa tgatccatgc gtacctcttc
      601 ttcgaacacc tcgttcccga cgaagtcgcc acgacctatg aggtcatcgc ccggttcctg
      661 cgcacgggcg aggcaacctg ggatccgtct tctcagtgaa ccgaacccgt tgaaccgaac
      721 cggtgcccgg cggctgccag ggacaccgcg taagatccgt cgcaccgact cgttgagtcg
      781 gcgacgagga gattgtcatg agtgagctgt tggccgggct gaagatcgat cacgtggcga
      841 ccccgcgtct gcgcacggcg gtgctggtcg acgaacgcca cgacggtgaa ccggtcgtgt
      901 tcgtgcacgg aaacgtgagt tcgtcgttgt tctggcagga atcgatcctg gcccttcccg
      961 acggattccg gggcatcgca ccggaccttc gcgggtttgg cgaatccgag accgcaccgg
     1021 tggatgcgac gcgtgggctc ggcgacttcg ccgacgacct gtactcgctg ctcgacaccc
     1081 tcgacatcga ccgagcccat ttcgtcggtt ggagcatggg cggcggcatc gtcatgcgca
     1141 tcctgctcga acgacccgaa atggtcgcaa gcctgaccct ggtcagcccc gtttccccct
     1201 acgggttcgg tggcacgaag ggggcatcgg gggaaccggt gaccgccgat ggcgccggag
     1261 ccggcggcgg cagtgcgaac cccgacttcg tggcggcgct cgctgcaggc gaccgaggtg
     1321 acgattcgcc gacgtcgccg cgcaacgtgc tcaacggtgc gtatttcgcc aacgagttcc
     1381 ggtcgccgct cgaagagacg ttcctcgact cgatgctcac caccaaggtc ggtgacgaca
     1441 actacccggg cgacgcgacc ggttcggaga attggccggg gttcgcaccg ggaacccgag
     1501 gcgtgctgaa cacgatggtg ccgggcgtcc acgacgtgtc cgggatcgtc ggggtctccc
     1561 cgaagccgcc gatcctgtgg gtgcacgggt ccaaggacgt catcgtgtcg gacgcctcga
     1621 tgttcgatct ggcgacgctg ggccgcatgg acgtcgtacc cggttggccg ggcgaggatg
     1681 cctgcccacc ccagccgatg gtgacccaga cccgggcggt gctcgacgac tacgccgcgg
     1741 ccggcggttc ctatcgcgaa gtcgaactcg attgtggcca cagcgccatg gtggaacgac
     1801 ccgacgaatt ccaagcggcg ctcgcacagg tcctgcgcgg ctgacgccgg gtgacgccgg
     1861 gttgcggccg ggttgcggcc acgctgccgc aacgacgctg ccgcaacccg gacctcgacg
     1921 acccggacct cgaccactcg ttcaggtcgt accgaacgga tcgctccacc cgttggactc
     1981 caggatcgag cgttgatggt cgacgaacga cgccatctcc acctgccccc agccgtccac
     2041 acttccggtc ccgtcgagct ggcggacgtt tcggccgagg gcgatcagca actcgatgct
     2101 tcgtgtgatc tgcccgctca gcgtggcggg atcggcggag cgaagtgccc gcaattccat
     2161 ttttgcctga agcattgagc cggctttgct cccgacgtca gtgaccttgc caagcgtgat
     2221 gacgatctga tccttcaatt cagcggccgc cttgtcggct ccgctgacgg accgctgggc
     2281 atgggctatc actccagggg ggcctgtttc agagggaacc ggccctgcc
//
LOCUS       DAOOMA010000462         2326 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_57173_length_2326_cov_4.451783, whole genome shotgun sequence.
ACCESSION   DAOOMA010000462 DAOOMA010000000
VERSION     DAOOMA010000462.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 2326)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2326)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2326
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57173_length_2326_cov_4.451783"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..518)
                     /locus_tag="PLP95_11215"
     CDS             complement(<1..518)
                     /locus_tag="PLP95_11215"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012224036.1"
                     /note="catalyzes the reversible transfer of the terminal
                     phosphate of ATP to form a long chain polyphosphate;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA degradosome polyphosphate kinase"
                     /protein_id="HPB46414.1"
                     /translation="MTTTRFNNRELSWLDFDARVLALATDPAVPVLERAKFLAIFGQN
                     LDEFYQVRVAGLLDQVEAGIVEPTPDGMTPAQQLAEISDRVEELVARADEVFVHGLLP
                     ALNAEGISFCTWDQLDVDNRRHLRQVFDDRILPILTPLAVDPAHPFPEISNLSLNLAL
                     RVVDPDNSEERFA"
     gene            864..1652
                     /gene="pstB"
                     /locus_tag="PLP95_11220"
     CDS             864..1652
                     /gene="pstB"
                     /locus_tag="PLP95_11220"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HPB46415.1"
                     /translation="MTEAVFETKNLSVLYSGFRAVRDVNMTIRRGEITAFIGSSGCGK
                     STVLRCLNRMNDLVEGASVEGQVLYRGVDLYDPEVSPVEVRRRIGMVFQKPNPFPKSI
                     WDNIAYGPRIAGVKKKQDLDDVVEKSLRRAALWDEVKDRLNTSAMGMSGGQQQRLCIA
                     RAIASEPEVLLMDEPCSALDPIATNRVEDLMRELRDDYTIVIVTHNMQQAARVSDTTA
                     FFGVEVSAEGDRRTGLLVEFDETERIFSSPADERTENYITGRVG"
     gene            1655..>2326
                     /gene="phoU"
                     /locus_tag="PLP95_11225"
     CDS             1655..>2326
                     /gene="phoU"
                     /locus_tag="PLP95_11225"
                     /inference="COORDINATES: protein motif:HMM:TIGR02135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0051049 - regulation of transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HPB46416.1"
                     /translation="MDDTDLRIEFHESLDELRDDIIRLGAMTVEMIGKGTAAMLDQDL
                     HAAQQIIDADDAIDMLEITIEESCYRMLALQQPMARDLRFIICCLRLANELERSADLM
                     VNVCKASRRMYDVTMSPQVRSYIEDMSIEAASLTRKAIDAFVESDAAKANALDDIDDR
                     LDLLQVEYMNAIINSHDTGAHGLSSAVQLALIGRYYERIGDHAVNMGERVAFMVSGWL
                     PEHAAI"
BASE COUNT          445 a          714 c          782 g          385 t
ORIGIN      
        1 gcgaatcgct cctcggagtt gtcggggtcg acgacgcgca gggccaggtt gagcgacagg
       61 ttggagatct cgggaaaggg gtgggccgga tcgaccgcga ggggggtgag gatcggaaga
      121 attcggtcgt cgaacacctg gcgcagatgg cggcggttgt cgacgtcgag ctgatcccac
      181 gtgcaaaagc tgatgccctc ggcgttcagc gcgggcagca atccgtggac gaagacctcg
      241 tcggcgcggg cgacgagttc ctcgacccga tcggagatct cggcgagctg ctgggcgggg
      301 gtcatcccgt cgggggtcgg ctcgacgatg ccggcttcga cctggtcgag caggccggcg
      361 acacgcacct ggtagaactc gtcgaggttc tggccgaaga tggcgagaaa ctttgcccgt
      421 tcaagcaccg gaaccgcggg gtcggtggcc agcgccagca cccgggcatc gaagtcgagc
      481 cagctgagtt cccggttgtt gaaacgcgtc gtcgtcaccg cacccacggt accgacgcgc
      541 cgcctcgatg cctacggcgc gaggtcccga agttgcagat tttgtggacc gttcacctgc
      601 tgttcacctc cggggcggcg aaggtacaca tagcggccgt aacgtaccca tcgtcccacc
      661 ggacaccggc tgcggccgag cccgccacgg tgtgccggat gagtgtgccc ggatgggtgt
      721 gccagatgag tttgaccccc gaccaaggac cgagtctgat gacgatgaac gacaatggca
      781 acgccacggt gcgaaccgca cgcgccgccg ttcgcagcgg cccggtggaa acgcctgccg
      841 gggctgagga gattcccgac atcgtgaccg aagccgtctt cgaaaccaaa aacctgtccg
      901 tgttgtactc cgggttccgt gcggtgcgcg acgtcaacat gacgattcgc cgcggcgaga
      961 tcaccgcctt catcggctcg tcggggtgcg gcaagtcgac ggtgctgcgg tgcctcaacc
     1021 ggatgaacga tctcgtcgag ggtgcgagcg tcgaggggca ggtgctgtac cggggagtcg
     1081 atctttatga ccccgaggtc agcccggtcg aggtgcgacg ccgtatcggc atggtgttcc
     1141 agaagcccaa tccgttcccg aagtcgatct gggacaacat cgcctacggc ccccggatcg
     1201 ccggagtcaa aaagaagcag gacctcgacg atgtcgtcga gaagtcgctg cgtcgggcgg
     1261 cgttgtggga cgaggtcaag gaccgcctga acacctcggc gatgggcatg tcgggcgggc
     1321 aacagcagcg cttgtgcatc gcccgggcga tcgcttcgga gcccgaggtg ctgctgatgg
     1381 acgagccgtg ttcggcgctc gacccgatcg ccaccaaccg cgtcgaggat ctcatgcgcg
     1441 aattgcgcga cgattacacc atcgtgatcg tgacccacaa catgcaacag gctgcccgtg
     1501 tgtccgacac gaccgcgttc ttcggggttg aggtgagcgc cgaaggcgac cgccgtaccg
     1561 gacttctcgt cgagttcgac gagaccgaac ggatcttttc cagcccggcc gacgaacgaa
     1621 ccgagaacta catcaccggc cgagtggggt gaccatggac gacaccgatc tgcgaatcga
     1681 atttcacgag tcgctcgacg aactgcgcga cgacatcatc cgcctgggtg cgatgaccgt
     1741 cgaaatgatc ggcaagggca ccgcggcgat gctcgaccag gacctgcacg ccgcccagca
     1801 gatcatcgac gccgacgacg cgatcgacat gttggagatc acgatcgagg aatcgtgcta
     1861 ccggatgctg gccctccaac agccgatggc ccgagacctg cgtttcatca tctgttgtct
     1921 gcggctcgcc aacgaactcg agcggtcggc cgacctcatg gtcaacgtgt gcaaggcgtc
     1981 gcggcgcatg tacgacgtga cgatgtcacc gcaggtgcgc agctacatcg aggacatgag
     2041 catcgaggcg gcgtcgctga cccgaaaggc gatcgacgcg ttcgtcgaga gcgacgcggc
     2101 gaaggcgaac gcgctcgacg acatcgacga ccggctcgac ctgttgcagg tcgagtacat
     2161 gaacgccatc atcaactccc acgacaccgg cgctcacggg ttgagctcgg cggttcagct
     2221 cgcgctcatc ggccgttact acgaacgcat cggcgatcac gcggtgaaca tgggcgagcg
     2281 ggttgcgttc atggtgagcg gttggcttcc ggaacatgcg gcgatc
//
LOCUS       DAOOMA010000463         2323 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_57294_length_2323_cov_3.969136, whole genome shotgun sequence.
ACCESSION   DAOOMA010000463 DAOOMA010000000
VERSION     DAOOMA010000463.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 2323)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2323)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2323
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57294_length_2323_cov_3.969136"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..223
                     /locus_tag="PLP95_11230"
     CDS             <1..223
                     /locus_tag="PLP95_11230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB46417.1"
                     /translation="GLTENIATFRQAMTEAGRNPDDMEIVPFGSIPSPEKLDHFEHIG
                     VTECVFRVPSAGRDEVLVTLDEQAALIAG"
     gene            complement(227..2170)
                     /locus_tag="PLP95_11235"
     CDS             complement(227..2170)
                     /locus_tag="PLP95_11235"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HPB46418.1"
                     /translation="MATLSDIVAVEAEFTALLNNPRRSLSRFAEVSLDFGQMLAEPDP
                     HRALEALSLAIETLRNPPAEPEIPVALGLLRTMRSVAARLGEADHELDAIGREGRILD
                     GGLVEIAPGEREALWQRAAAVRFGEGDVAAGFGELMEGLRWSGPVGIEPLTTDLLIAV
                     RRTQLPAELLNDLIELWFEIRRERAGDAYGPWCLVALGALQLTAGDTITAQMYMRQAD
                     KMFGNEQRPALAALVGGWVALSADDAPTALNRFVSASQAPGGDARIRLLAAAGLGEAL
                     VALGRVDEARKPLNEAIAYDVGDPNTVARSHELLSEIAAGEGRYEDAYQHLTVARRLE
                     QPMVVHSTPQRVHGVPTELDLRDRSEPIVLPSSPLRTASEEIAAHARSVERAAREASA
                     AAGLDATLEMHLSPILENASGDVRAALAVMWLVHPDGAAIPAATLSEREELRRQLALH
                     TLRRGCRSLATWASDLMMVIDLSEISVTSELVEAVETQLRESGADASRLVVLLSGLRL
                     LRSSERSAVVDGLRDLGVRLGITGLGSSFDSTEELLSWGVDLVVIDDEVLSLADRGRA
                     QRVVATVSALANGFGFDLLATGVGNEESFARQVSAGCHAAVGPHVGPALSEAAFRHQH
                     LIDTAIDSAIGSVAGAVAGSALT"
BASE COUNT          436 a          834 c          712 g          341 t
ORIGIN      
        1 cgggctcacc gagaacatcg ccacgttccg ccaggcgatg accgaggccg gacgcaatcc
       61 cgacgacatg gagatcgtgc ccttcggttc gattcccagc cccgaaaaac tcgaccattt
      121 cgaacacatc ggggtgaccg aatgtgtctt tcgagttccg tcggccggcc gcgatgaggt
      181 actcgtcacc ctcgacgagc aggccgcgct gatcgccggt tagcgctcag gtcagcgctg
      241 accccgcaac cgcgccagcc acggatccga tcgcggaatc gatggctgtg tcgatgaggt
      301 gctggtgacg aaaggccgcc tccgacagtg ccgggccgac gtgaggcccg accgcagcgt
      361 ggcacccggc actaacctgg cgggcgaacg attcctcatt accgacgccc gtagcgagca
      421 gatcgaatcc gaacccattc gccagtgccg agacggtggc caccacgcgc tgggcgcgcc
      481 cacggtcggc gagcgacaac acctcgtcgt cgatcaccac caggtcgacc ccccacgaca
      541 acaactcctc ggtcgagtcg aatgacgagc cgagccccgt gatcccgagc ctcaccccaa
      601 ggtcgcgaag gccgtcgacg accgccgaac gttcagacga acgaagcagc cgcagcccgg
      661 acaacagcac cacgagacgc gaagcatcgg ccccggactc gcgcaactgt gtttcgaccg
      721 cctcgacaag ttccgaggtc accgagatct cggacagatc gatgaccatc atgaggtcgg
      781 atgcccacgt cgcgagagag cggcacccac gccgcagcgt gtgcagcgcc agctgccgac
      841 gaagctcctc gcgttcggac aaggtcgccg cgggaatcgc tgcaccgtcg gggtgcacca
      901 gccacatcac cgccaaggcc gcccgcacgt cgcccgacgc gttctccagg attggcgaca
      961 ggtgcatctc caacgtcgcg tccaaccctg cggcagcgga ggcctcgcgc gccgcacgct
     1021 cgaccgagcg ggcgtgcgcc gcgatctcct cgctcgcggt gcgcagcggc gacgacggca
     1081 acacgatcgg ttccgaacgg tcacgcaagt cgagctcggt cggcaccccg tgcacacgct
     1141 gcggtgtgga atgcaccacc atcggctgtt cgagacgacg ggccacagtc aggtgctggt
     1201 acgcgtcctc gtatctcccc tcgccagcag cgatctccga cagcagttcg tggctccggg
     1261 cgacggtgtt cggatcgccg acgtcatacg cgatcgcctc gttgagcggc ttgcgggcct
     1321 cgtcgacgcg cccgagcgca accaacgcct cacccagtcc ggccgcggcc aacagtcgaa
     1381 ttcgagcatc gccaccgggc gcctgggacg cactcacgaa ccggttcagc gccgtcgggg
     1441 catcgtctgc agacaacgca acccaaccgc cgaccaatgc ggcgagcgcc gggcgctgtt
     1501 cgtttccgaa catcttgtcg gcctggcgca tgtacatctg tgcggtgatc gtgtcccccg
     1561 ccgtgagctg cagcgcaccg agcgcgacga ggcaccacgg cccgtaggca tctccggctc
     1621 gttcgcggcg aatctcaaac cacaactcga tgagatcgtt gagcagttcc gccggaagct
     1681 gtgtgcgccg aaccgcgatc aacaagtcgg ttgtgagcgg ctcgatgccg acggggccgc
     1741 tccagcgcaa accctccatc aactccccaa atcccgcggc cacgtcgcct tcgccgaatc
     1801 ggaccgcagc ggcccgctgc cacagcgcct cgcgttcgcc gggggcgatt tcgacgagac
     1861 cgccgtcgag gatccgccct tcacggccga tcgcatcgag ttcgtgatcc gcttcaccga
     1921 gccgtgccgc gaccgaacgc atcgtcctca gcagccccag cgcgaccgga atctccggct
     1981 ccgccggagg attgcgcaac gtctcgatcg ctagcgacag cgcctcgagt gcccgatgag
     2041 ggtcgggttc ggccaacatc tgtccgaagt cgaggctgac ctcggcgaaa cgcgacaggc
     2101 ttcgacgagg attgttgagc agtgccgtga attcggcctc gaccgcgaca atgtcggaca
     2161 gcgtcgccat gtcagcgcac ctcgaacgcc aggaaccacc gacgactcac gggttgaaat
     2221 gctagtgacg ccccagagcg cgatcgggga tcctcaggtc accgagatac cagcgccgcc
     2281 agtgcgcgac gtcgtcagct tcgacggagt tgattccacg ggg
//
LOCUS       DAOOMA010000464         2320 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_57422_length_2320_cov_3.282561, whole genome shotgun sequence.
ACCESSION   DAOOMA010000464 DAOOMA010000000
VERSION     DAOOMA010000464.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 2320)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2320)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2320
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57422_length_2320_cov_3.282561"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..722
                     /locus_tag="PLP95_11240"
     CDS             <1..722
                     /locus_tag="PLP95_11240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020378333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HPB46419.1"
                     /translation="RLGDGVEGLRVGLPRELLGEGIAPEIRARIDEAAAALASAGATI
                     EEVSIPTVEQALSAYYILAPAEASSNLSRFDGVRFGQRVDAATTPEMYDRSRTAGFGA
                     EVKNRIMLGTYALSAGYYDAYYGTAQKVRTLVMNDFAAAYGTVDVLLTPTSPTTAFAI
                     GANVDDPLTMYLNDICTIPTNLAGHPAMSVPFGADGDGLPIGVQVLAPALGEVAMFRA
                     AAVLEAAAPALRTPNPLEVSK"
     gene            821..1666
                     /gene="gatB"
                     /locus_tag="PLP95_11245"
                     /pseudo
     CDS             821..1666
                     /gene="gatB"
                     /locus_tag="PLP95_11245"
                     /inference="COORDINATES: protein motif:HMM:NF014930.2"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
     gene            1883..2218
                     /locus_tag="PLP95_11250"
     CDS             1883..2218
                     /locus_tag="PLP95_11250"
                     /inference="COORDINATES: protein motif:HMM:NF014674.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB46420.1"
                     /translation="MNATRFAELIAMEVGGAVTATQAKTVLAEMLDRGPGADPAAIAA
                     ELGFEAMDSGEIEALVDDLIAKHPEEFQRFCDGDGKVVGFFVGQVMKATQGKADGKAV
                     TAILNGHKP"
BASE COUNT          396 a          776 c          793 g          355 t
ORIGIN      
        1 atcgcctcgg cgacggggtc gagggcctgc gggtcggcct gccacgagaa ctgctcggcg
       61 aggggatcgc ccccgagatc cgcgctcgaa tcgacgaggc cgcagcggca ctcgcctccg
      121 ccggggcaac gatcgaggag gtctcgattc ccaccgtcga acaggccctg agcgcctact
      181 acatcctggc cccggcggag gcgagttcga acctcagccg cttcgacggc gttcggttcg
      241 gccagcgtgt cgacgctgcg accactccgg agatgtatga ccgcagccgc actgcggggt
      301 tcggtgccga ggtcaagaac cgcatcatgt tgggcaccta cgcactgtcg gcgggctact
      361 acgacgccta ctacggcacc gcacagaagg ttcgaacgct cgtgatgaac gacttcgcgg
      421 ccgcctacgg caccgtcgac gtgttgttga ccccgacctc gccgaccacc gcattcgcga
      481 tcggtgccaa cgtcgatgac ccgctgacca tgtacctcaa cgacatctgc accattccga
      541 ccaacctggc ggggcacccg gcgatgtcgg tgccgttcgg ggctgatggc gacgggttgc
      601 cgatcggcgt gcaggtcctc gcccccgcac tcggcgaggt ggcgatgttt cgcgccgccg
      661 cggtgctcga agctgctgcg ccggcgttgc gaacgccgaa tccgttggag gtgtcgaagt
      721 gagcgacgtc atcaagccgt cggtcggcgg caccggcgag gtgttcacca ccgatcgccc
      781 acagcagccc cagaacccgg tgctgccgga ggggtgggag atggtgatcg gccttgaggt
      841 gcacgccgag ctgtcgaccc gcaccaagtt gttcagcgga tcgctcaacg agttcggcgg
      901 cgaccccaac accaacatcg acccggtgag cctcgggctg ccgggggcgc ttccggtgct
      961 caaccgtcac gccgtcgagt tggccatccg caccggcctg gcgctcaatt gcacggtcgg
     1021 ccgttcggtg ttcgcccgga agaactattt ctaccccgat atgcccaagg ggtatcagat
     1081 cagccagtac gaccttccga tcaaccgcga cggctggctg gagttgcccg acggcacccg
     1141 catcggcatc gagcgcgccc acctcgagga ggacaccggc aagtcgaccc acatgggcgg
     1201 ggagtccggc cgaatccacg acgcgcagta ttccctgatc gatttcaacc gcgccggtgt
     1261 tccgttgctg gagatcgtca gccggcccga cctgcgcacc gcggaggagg ccaaggcgta
     1321 ggtgtcggaa ttgcgggaca tcctcgtcgc ggtcggcgcc tccgacggca agatggagga
     1381 gggttcgatg cgggtcgact gcaacgtcag cgtccgtcgc atcggcgacg gcctcggcac
     1441 ccgctgtgag atcaagaacg tgaactcgct gcggtcgctc gggcgcgcca tcgactacga
     1501 ggcccgccgc cagatcgatc tcatcgacgc tggggaggcg gtgcaacaag agacccgtca
     1561 ctggaacgac ggggccgggc gcacctcgac gctgcgcacc aaggaggagg cgaccgacta
     1621 ccgctacttc ccggaaccgg atctggtggt cgtcgatccg gatgaggcgt ggatcgaatc
     1681 ggtgcgggcg gccctgccgg cgttgccccg cgagcgtcga gtcgcactgg tcgaggcaac
     1741 cggtgccgga accgaggcgg cggcgctcat cgtgacccgc gacctcgacg cgctggtact
     1801 cgaggccatc gggttcggtg ccgatcccgg gcgtgccgcc acccatgcgg aacacaacct
     1861 caccgacgaa cgtgcccgcg acatgaacgc gacccggttt gccgaactga tcgccatgga
     1921 ggtcggcgga gcggtcaccg ccacgcaggc caaaacggtg ctcgcggaga tgctcgatcg
     1981 cggaccgggc gccgacccgg cggccattgc cgcggaactg gggttcgagg cgatggacag
     2041 cggcgagatc gaggccctcg tcgacgacct gatcgccaag caccccgagg agttccagcg
     2101 attctgcgac ggcgacggca aggtcgtcgg attctttgtc ggtcaagtca tgaaggcgac
     2161 ccagggcaag gcggacggca aggcggtcac cgcgatcctg aacggtcaca agccgtgatc
     2221 gtggccggtg tgaaacggcg gccggtggcg cgcgctgtgc tctgtgcagt ggtgaccgca
     2281 gcgttgtcgg tgccgacgct cgcgtgcagc tccgacgacg
//
LOCUS       DAOOMA010000465         2317 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_57545_length_2317_cov_3.029178, whole genome shotgun sequence.
ACCESSION   DAOOMA010000465 DAOOMA010000000
VERSION     DAOOMA010000465.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 2317)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2317)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2317
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57545_length_2317_cov_3.029178"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..602
                     /gene="prcA"
                     /locus_tag="PLP95_11255"
     CDS             <1..602
                     /gene="prcA"
                     /locus_tag="PLP95_11255"
                     /EC_number="3.4.25.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015798345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005839 - proteasome core complex
                     [Evidence IEA];
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0010498 - proteasomal protein catabolic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="proteasome subunit alpha"
                     /protein_id="HPB46421.1"
                     /translation="LVAVTYADGILLCAENPSRMLRKISEIYDRIGFAGVGKYNEFDQ
                     LRIAGVRHADLKGFAFSREDVDARSLANQYAQMLGQVFTHEMKPLEVEILVAEVGSSA
                     EHDQLFHIHYDGTLVDEANFTVLGGDAEAIRERLADAFVAGAPLADALAGAVGALEGP
                     DRRLPEPELEVAVLERQAPRRSFRRITGDELAALLPPEE"
     gene            607..1965
                     /gene="pafA"
                     /locus_tag="PLP95_11260"
     CDS             607..1965
                     /gene="pafA"
                     /locus_tag="PLP95_11260"
                     /EC_number="6.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0010498 - proteasomal protein catabolic
                     process [Evidence IEA];
                     GO_process: GO:0019941 - modification-dependent protein
                     catabolic process [Evidence IEA];
                     GO_process: GO:0070490 - protein pupylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Pup--protein ligase"
                     /protein_id="HPB46422.1"
                     /translation="MQRRIVGLESEYGVTCTLPGQRRLSPDEVARYLFRKVVSWGRSS
                     NVFLGNGARLYLDVGSHPEYATPECDSLHDLVAHDKAGERILEGLVTSAEERLRQEGT
                     DAEIYLFRNNTDSAGNSYGCHENYLTTRDDTVNRYDEVLIPFLISRQIYSGAGKVLNT
                     ARGPLYCLSQRGEHIWEGVSSATTRSRPIINTRDEPHADVDKYRRLHVIVGDTNMGEY
                     PTFLKVGTTSLILRMLEDRNVVLRDLTLDNPIRAIREISHDMTCSVRVRLANGREASA
                     FELQSEFLSRAQRYAQSHDMTPQEKQSLAMWEHVMAGIERDPLSLERELDWVTKFNLI
                     DAYRQRHQLAMGAPEVALIDLQYHDISRHRSVFYKLQRRDLVERIVTDQDIADAVDTA
                     PQTTRARLRGEFIKRAKERRRDYTVDWVHLKLNDDAQRTVLCKDPFKSHDERVDHLIA
                     SL"
     gene            2000..>2317
                     /locus_tag="PLP95_11265"
     CDS             2000..>2317
                     /locus_tag="PLP95_11265"
                     /inference="COORDINATES: protein motif:HMM:NF024384.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3866 family protein"
                     /protein_id="HPB46423.1"
                     /translation="MPHFRTGTVTSVVSERAGLQRVEVLMPDGATARAYVLSDLLGTV
                     AVGDEVVCNTTAVDLGLGTGGSHVVHWNLSRRELHLPGPDHVMKLRYTSLQSDVGTSE
                     LLHP"
BASE COUNT          452 a          784 c          704 g          377 t
ORIGIN      
        1 cactcgtggc cgtcacctat gccgacggca tcttgttgtg tgccgagaac ccgagccgca
       61 tgctgcgcaa gatctccgag atctatgacc gcatcgggtt cgccggggtc ggcaagtaca
      121 acgaattcga tcaactccgc atcgccggcg tgcgccacgc agacctgaag gggttcgcct
      181 tcagccgtga agacgtcgat gctcgaagcc tggcgaatca gtacgcgcag atgctcggac
      241 aggtgttcac ccacgagatg aagcccctcg aggtcgagat cctcgtcgcc gaggtcggct
      301 ccagcgcgga acacgaccag ttgttccaca tccactacga cgggacgctc gtcgacgaag
      361 cgaatttcac cgtgctcgga ggcgatgccg aggccattcg cgaacgtctc gccgacgcgt
      421 tcgtcgcagg ggcaccgctc gccgacgcgt tggccggggc ggtcggcgcg ctcgaaggcc
      481 cggaccggcg tctccccgag cccgaactcg aggtcgcggt gctcgaacgc caggcgcccc
      541 gccgcagttt ccgacgtatc accggcgacg agttggcggc gctgctgccc cccgaggagt
      601 aggacggtgc agcggcgcat cgtcgggttg gaaagcgagt acggcgtcac gtgcacgctg
      661 cccggccagc gccgtctttc cccagatgag gtggcccgct acctgtttcg taaggtggtg
      721 tcgtggggcc gttcgtccaa cgtgttcctc ggcaacgggg cacgtctgta cctcgatgtc
      781 ggctcccatc ctgaatacgc cacccccgaa tgcgattcgc tgcacgacct cgtcgctcac
      841 gacaaggcgg gcgaacgcat tctggagggc ctcgtcacgt cggcagagga acgccttcgc
      901 caagaaggca ccgacgccga gatctacctg tttcgcaaca acaccgactc ggccggcaac
      961 tcctacgggt gccatgagaa ctacctcacg acccgcgacg acaccgtcaa ccgttacgac
     1021 gaggtgttga tcccattcct gatcagccgt cagatctaca gcggcgccgg aaaggtgctc
     1081 aacaccgccc ggggaccgct gtattgcctg agccaacgcg gcgaacacat ctgggagggc
     1141 gtgagttcgg ccacgacccg cagccggccg atcatcaaca cccgcgacga accccacgcc
     1201 gatgtcgaca agtaccgtcg cctccacgtc atcgtcggtg acacgaacat gggggagtac
     1261 ccgacgtttc tcaaggtggg cacgacctcg ttgatcctgc gcatgctcga ggaccgcaac
     1321 gtcgtgttgc gcgacctcac cctcgacaac ccgattcgcg ccatccgcga gatcagccac
     1381 gacatgacct gttcggttcg ggtccgactc gcaaatggcc gcgaagcgag cgcgttcgaa
     1441 ctgcagagcg agttcttatc gagggcgcag cgctacgcac aatcacacga catgaccccg
     1501 caggaaaagc agtcgctcgc catgtgggaa cacgtgatgg ccggcatcga acgcgatccg
     1561 ctcagcctcg aacgcgaact cgactgggtc accaagttca acctgatcga cgcctaccgg
     1621 cagcgccatc aactcgcgat gggggcaccc gaagtggcgc tgatcgatct tcagtaccac
     1681 gacatcagcc ggcaccgttc ggtgttctac aagctgcagc ggcgcgacct ggtcgaacgg
     1741 atcgtgaccg accaggacat cgccgatgcc gtcgacaccg caccccagac cacccgtgcc
     1801 cgactgcggg gcgagttcat caaacgcgcc aaggaacgcc gccgcgatta caccgtcgac
     1861 tgggttcacc tgaaactcaa cgatgacgca cagcgcaccg tgttgtgcaa ggacccgttc
     1921 aaatcgcacg acgaacgggt ggatcacctg atcgcgtcgt tgtgatcgtc gacgcctgag
     1981 aacgaaccac aggagggtcg tgcctcactt tcgaaccgga accgtcacct cggtggtatc
     2041 agagcgcgcc gggttgcaac gcgtcgaggt gctgatgccc gacggggcca cggcgcgggc
     2101 ctatgtcctg agcgacctcc tcggaacggt tgcggtcggc gacgaggtcg tgtgcaacac
     2161 caccgcggtc gatctcggcc tcggaaccgg gggctcccat gtcgtgcact ggaacctgtc
     2221 gcgacgcgaa ctgcacctgc ccggacccga ccacgtcatg aaactgcggt acacctcgct
     2281 gcagagcgat gtgggcacct cggagctgtt gcacccg
//
LOCUS       DAOOMA010000466         2315 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_57608_length_2315_cov_4.564159, whole genome shotgun sequence.
ACCESSION   DAOOMA010000466 DAOOMA010000000
VERSION     DAOOMA010000466.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 2315)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2315)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2315
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57608_length_2315_cov_4.564159"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..264
                     /locus_tag="PLP95_11270"
     CDS             <1..264
                     /locus_tag="PLP95_11270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB46424.1"
                     /translation="MQMPQPSYLTNRWLSAILCVAIGLDRAHTGAVASVEVSVERHDL
                     VLSLTPSNDSDLALERYAAIERRALLAEVAGRTVSVTTPPVLR"
     gene            complement(316..684)
                     /gene="mscL"
                     /locus_tag="PLP95_11275"
     CDS             complement(316..684)
                     /gene="mscL"
                     /locus_tag="PLP95_11275"
                     /inference="COORDINATES: protein motif:HMM:NF013869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HPB46425.1"
                     /translation="MLAGFKKFIMQGNVVDLAVGVAIGAAFGALVTSFTTSFLDPLIR
                     LITGGGTTGGTFTINDQVFDYGSFITAIITFVITAAAIYFVVAVPMNTIKERRSNSAE
                     PEDPTNEEKMIALLEQIAKK"
     gene            complement(782..1255)
                     /gene="greA"
                     /locus_tag="PLP95_11280"
     CDS             complement(782..1255)
                     /gene="greA"
                     /locus_tag="PLP95_11280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015798937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0006354 - DNA-templated transcription
                     elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HPB46426.1"
                     /translation="MTTSEHHLSQAAHDRLKAELDELTTVGRIEIARKIEAAREMGDL
                     SENGDYHAAKEEKGKMEARIGHLSALLERSTIIVEDGPLDEVVAGCVVTLIYEGDDDE
                     EQYLIGSIEERRDGVAIVSPSSPLGSALLGAKPGERVSFEAPNGELTVEILRLDR"
     gene            1386..>2315
                     /locus_tag="PLP95_11285"
     CDS             1386..>2315
                     /locus_tag="PLP95_11285"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HPB46427.1"
                     /translation="MATHRIAVVGGDGIGPEVTAEALKVLDATGVSYERIDYDLGAAR
                     YLRDGTVLPDSVLDEWRQVDAILLGAVGDPAPGVSAPAGLVERGVLLRTRFELDMYIN
                     LRPFRLAPKTDFEVIRENTEGTYAGEGGFLRKGTPFEIATQGSVNTRKGAERAIRYAF
                     DRAMQTERKTCHLIHKKNVLTFAGDLWQRTFDIVAADYPEVATGYDHIDAACIHFVER
                     PERYDVIVTDNLFGDILTDLGGAVAGGVGFAASANLNPDRTGPSLFEPVHGTAPDIAG
                     QNKANPIAAIASAAMMLEFLGEADAAARVAAACE"
BASE COUNT          402 a          726 c          774 g          413 t
ORIGIN      
        1 atgcagatgc cgcaaccgtc ctacctgacc aatcgctggc tgtcggcaat cctgtgcgtg
       61 gccatcgggc tcgaccgcgc tcacaccggt gccgttgcgt cggtggaggt ctcggtcgaa
      121 cgccacgacc tggtgttgtc gctgaccccg tcgaacgaca gtgatctggc gttggaacgc
      181 tatgccgcga ttgagcgcag agcgctgctg gccgaggtgg ccggtcggac cgtgagcgtc
      241 acaacaccgc ctgttctccg ataaccagtg gtcgtgatca cgaccactgg ttatcggaga
      301 acaggctggg gcgggctact tcttggcgat ctgttcgagg agggcgatca tcttctcctc
      361 gttggtcggg tcctcgggtt cggccgagtt ggagcgacgc tccttgatcg tgttcatcgg
      421 aacggcgacg acgaagtaga tcgccgcggc ggtgatcacg aaggtgatga tcgcggtgat
      481 gaaggacccg tagtcgaaca cctgatcatt gatcgtgaag gtgccaccgg tggtgccgcc
      541 accggtgatg agccggatca gcggatcgag gaacgacgtc gtgaacgacg tgaccagcgc
      601 gccgaacgcc gcgccgatgg caacaccgac ggcgaggtcg acgacgttgc cttgcatgat
      661 gaatttcttg aagcctgcga gcatggggaa agccctttcg ttgtgaacag gtgagatgac
      721 cgtagtcacg ctatgaacgc ggggtcccca aaatgcggat aggtgttttt cctaccgttc
      781 gtcagcggtc gaggcgcagg atctcgacgg tcaattcgcc attcggggcc tcgaaggaca
      841 cccgttcacc gggcttggcg ccgagcaacg ccgagccgag cggggacgac ggggagacga
      901 tcgcgacccc gtcgcggcgc tcctcgatgg aaccgatgag gtactgctcc tcgtcgtcat
      961 cgccctcgta gatgagagtg acgacgcagc ccgcgacgac ctcgtcgagc gggccgtcct
     1021 cgacgatgat cgtcgagcgt tccaacaggg cgctgagatg gccgatgcgg gcttccatct
     1081 ttcccttctc ctccttggcg gcgtggtaat cgccgttttc ggagaggtcg cccatctctc
     1141 gagccgcctc gatctttcgg gcgatttcga ttcggccgac cgtggtcaac tcgtcgagtt
     1201 ccgccttgag gcgatcgtgg gcggcctggg agagatggtg ctcggacgtg gtcatggccc
     1261 gcgatcctac cgacgatcac ggtctcacgg gcgcaggcaa cgacggccct cggcgcgacg
     1321 ggtaggcttt cacggtcaca tcgcgagccc gaagggctgg tggaacggtc gtcggaaagg
     1381 acctcatggc aactcacagg atcgcagtgg tcggaggcga cggaatcggc ccggaggtca
     1441 ccgcggaggc actcaaggtg ctcgacgcga cgggggtctc ctatgagcgc atcgattacg
     1501 acctgggcgc ggcccgctat ctgcgcgacg gcacggtgct gcccgattcg gtgctcgacg
     1561 aatggcggca ggtcgacgcc atcctgctcg gagcggtcgg cgaccctgcg cccggggtga
     1621 gtgctcctgc cggactggtc gaacgcgggg tgttgttgcg cacccggttc gagctcgaca
     1681 tgtacatcaa cctccgcccg ttccgcctgg ccccgaagac cgatttcgag gtgatccggg
     1741 aaaacaccga gggcacctat gccggcgagg gtgggttcct gcgaaagggc acgccgttcg
     1801 agatcgcgac ccagggttcg gtcaacaccc gcaagggggc cgaacgcgcc atccgctatg
     1861 ccttcgaccg ggcgatgcag accgagcgca agacctgcca tctcatccac aagaagaacg
     1921 tgttgacctt cgctggcgac ctgtggcagc gcaccttcga catcgtcgcc gccgactatc
     1981 cggaggtggc caccggctat gaccacatcg acgcggcgtg catccacttc gtcgagcgcc
     2041 ccgagcgata cgacgtgatc gtgaccgaca acctcttcgg cgacatcctc accgatctgg
     2101 gcggagccgt cgctggcggc gtggggtttg cggcctcggc caacctcaac cccgaccgca
     2161 ccgggccgtc gctgttcgaa ccggtgcatg gcacggcccc cgacatcgcc ggacagaaca
     2221 aggccaaccc gatcgccgcg atcgcatcgg cggcgatgat gctcgagttc ctcggcgagg
     2281 ctgacgcggc ggcgcgtgtc gctgcagcgt gcgag
//
LOCUS       DAOOMA010000467         2303 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_58076_length_2303_cov_3.104982, whole genome shotgun sequence.
ACCESSION   DAOOMA010000467 DAOOMA010000000
VERSION     DAOOMA010000467.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 2303)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2303)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2303
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58076_length_2303_cov_3.104982"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..386)
                     /locus_tag="PLP95_11290"
     CDS             complement(<1..386)
                     /locus_tag="PLP95_11290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB46428.1"
                     /translation="MTGSGSQYADLGTNAYGIVEVPDAAGIDLYEFTVIAQTAADSCW
                     PGSGKSTCIHSTASPAFQLEMPPVPTIAQHSSGHPMRPWRLRAQWSSSVTQGTWTLKR
                     SLVGVGGGPVTMDRVAVTGAAAESSQ"
     gene            903..1571
                     /locus_tag="PLP95_11295"
     CDS             903..1571
                     /locus_tag="PLP95_11295"
                     /inference="COORDINATES: protein motif:HMM:NF014566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HPB46429.1"
                     /translation="MPAFVAVVVVLAVVNLTRDHAPNGVWQVVVMAALGLVVWRAGLT
                     RDELGLGRGTARRGLGVGAIVFVGIAILVILGGALGLVTDERVAGSGAEAAWRIFVVI
                     PLGTVAVEEFAFRGVLFGLGERLWESRTALVVNAVLFGMWHLYPAATGGAVTSPDTAG
                     VEVAGPWLVAGTVLATTAAGFAFAGLRRTTKSVISPAFAHLALNSVGYAVAYCAGSRF
                     GGMT"
     gene            1568..>2303
                     /locus_tag="PLP95_11300"
     CDS             1568..>2303
                     /locus_tag="PLP95_11300"
                     /inference="COORDINATES: protein motif:HMM:NF014808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M17 family peptidase N-terminal
                     domain-containing protein"
                     /protein_id="HPB46430.1"
                     /translation="MKIEVSVGSVLESAASVGILGWLGDTDLPDSLASSVEAADFTGK
                     PNQTVVVYPRTEGLPTRLVLVGLGEADKVDGERIRQAAASAIRVARGFGEATVAMGLP
                     EGLVVAGAELGEAVAVGLLLGNYRFLRYRTNLSDAESHEIEAVEVLVGATELDAVSGG
                     VATGTIVGNGVVFARDLVNTPGAEKTPPQLAQRAVELGEQVPSISVTVLDENELEAQG
                     FGGVLAVGKGSDSPPRFIIMEYGADLE"
BASE COUNT          362 a          732 c          787 g          422 t
ORIGIN      
        1 aactggctgg actccgctgc cgccccggtc acagcgacac gatccatcgt gaccggtcca
       61 ccaccgacac cgacgagcga ccgcttcaac gtccaagttc cctgagtaac cgacgacgac
      121 cactgtgcac gcagccgcca cggacgcatc ggatgcccac tgctgtgttg agcgatggtc
      181 ggtaccggtg gcatctccag ctgaaacgcc ggcgaagccg tcgaatggat acaggtcgac
      241 ttgcccgacc ctggccagca actgtccgcg gcggtctgcg cgatgacggt gaactcgtac
      301 agatcgatcc ccgccgcgtc gggcacctct acgatgccgt acgcgttcgt gccgagatcg
      361 gcgtactgcg accccgatcc cgtcacccgc ttcttttcgg tctgatagcc gtactggccg
      421 tcaaccagcg tggatgagga gatcgttggc ttgtaggtgc cactgtttcc agtccatgac
      481 tacgacccgc ccgacggttt ccaccagatc tgatagctcg ccacggttgc gtcgaccggc
      541 gcccaccgca cacgaacacg gtggaagttc gggttcgcgt tcccgggacc ggaatcggta
      601 cccgaggcca acaaggcgac ctcggttatc accggcgtgg caacggccgc acctgccgct
      661 ggtgatgaca tgaccgacaa cgctgacgcc aagagcgtcg ttgtcacgat ggcagccgcc
      721 attgtgaatc gttcaaactt catcgcaccc ctccagaagt ccgctcgcgt gcgaaccctg
      781 tcggtaggcg cgccgagttg ttgagcttcc gaccggaagg gcccgcggca cccgtagccc
      841 gacagccgtg ccggcgactg ctggcgaccg ccggtagcgc ggccgggtag tgtcccgctg
      901 gcatgccagc gtttgtcgcc gtcgtcgtcg ttctggccgt cgtcaacctg acgcgtgatc
      961 acgctcccaa cggggtgtgg caggtcgtcg tgatggctgc gctcggtctc gtggtgtggc
     1021 gcgccgggtt gacccgtgac gaactcggct tgggtcgcgg aacagcgcgt cgtggactag
     1081 gggtcggggc gatcgtcttt gttggcattg cgatcttggt gatactcggc ggggcgcttg
     1141 ggctcgtcac cgacgagcgg gtcgccggca gcggcgccga ggcggcgtgg cgcattttcg
     1201 tggtcatccc gctcggcacc gtggcggtcg aggagttcgc gttccgggga gtgctgttcg
     1261 gtttagggga gcggctgtgg gaatcgcgga ctgcgctggt ggttaacgcg gtgctgttcg
     1321 gaatgtggca cctctacccg gcggcaaccg gcggggcggt gacgtcgccg gacacggcgg
     1381 gcgttgaggt ggcaggtccg tggctcgtgg ccggcacggt gctcgcgacg accgcagcgg
     1441 gtttcgcctt cgccgggctc cgccgaacga caaaaagtgt gatttcgccc gcgtttgcgc
     1501 acctcgctct caacagtgtt ggctacgctg tcgcgtactg cgcgggatcc cgttttggag
     1561 gaatgacatg aagatcgagg tgagcgtcgg gtcggtactg gaatctgccg catcggtggg
     1621 cattttgggt tggctcggcg ataccgacct tcccgattcc ctagcgtctt cggtggaggc
     1681 agccgacttt acgggcaagc cgaaccagac cgtcgtcgtc tacccccgca ccgagggcct
     1741 tccgacacgg ctcgtgctgg tggggttggg cgaggccgac aaggtcgatg gtgaacgcat
     1801 ccgccaggcg gccgcctcgg cgattcgtgt cgcgcgaggc ttcggcgagg ccacggtcgc
     1861 gatggggctt cctgaagggc tcgtggtcgc cggggccgaa ctcggcgagg cggtggccgt
     1921 cgggctcctg ttaggcaact accgcttcct tcgctatcgc accaacctga gcgacgccga
     1981 gtcccacgaa atcgaggcgg tggaggtgct cgtcggcgca actgaactcg acgcggtgag
     2041 cggcggtgtt gcaaccggca cgatcgtcgg caacggcgtc gtgttcgcac gcgacctcgt
     2101 caacaccccg ggggctgaga agactccgcc acaacttgcc cagcgggccg tcgaactcgg
     2161 cgagcaggtg ccgtccatct cggtgacggt gctcgacgag aacgaactcg aagcgcaggg
     2221 attcggggga gtcctcgccg tgggcaaggg ttcggacagc ccgccgcgct tcatcatcat
     2281 ggaatacggc gccgacctcg aag
//
LOCUS       DAOOMA010000468         2299 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_58202_length_2299_cov_4.969697, whole genome shotgun sequence.
ACCESSION   DAOOMA010000468 DAOOMA010000000
VERSION     DAOOMA010000468.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 2299)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2299)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2299
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58202_length_2299_cov_4.969697"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2299)
                     /locus_tag="PLP95_11305"
     CDS             complement(<1..>2299)
                     /locus_tag="PLP95_11305"
                     /inference="COORDINATES: protein motif:HMM:NF012961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycoside hydrolase family 9 protein"
                     /protein_id="HPB46431.1"
                     /translation="DPSVGLGEWRDLATTVTLDASEGYQEVFARFRDSTASGSGASPT
                     DFTVAGITIDSGYEAATADPPVPRSIGLITPQILDVVVEIGRIDRGISNDGDTYTGAT
                     VEPTRFDSGWKISGPNAPTLSFVDRISTPTDGGRDATGDMKLFAVRHEVRLGLSAPLE
                     PGATYTVTSPVGSTIELVADDHLAHSPAVSVNQAGYTSTGTKIATYAIPNALGSTSSP
                     TAVEFAIVDSEGREHFRGTGVAAPSGSEAGKGDLTDAAVWRLRFDEVATAGRYRVCVD
                     EVGCSPGFAVDDAIWSRLAATVAHSLYYQRSGIELNQPYGSFSRPRTNHPDDYTTVLE
                     TNLTGFDASEQTDETLFDSITAEATTTPVADAWGGHFDAGDWDRRTQHLFMVRTLFDL
                     ARRYPEAFPDGSLNLPESGNGISDLVDEALWTVDLFRRLQREDGAVRGGIESERFPDA
                     GIASWQDTITRYAFAPDPWSSWVYASVAADASMLLAGIDDQRSVEYASSAVRAWEWAA
                     TQPVPTTDGDPANAKVLSQRAGAAAALYDLTGEDRFVQSFAADVPFRDLPASTLSCHS
                     FEWCDAGWRYLLIDQARTDATLRANIEASFASVASAIADASDTTRYGWCLEDPGVPMV
                     WGMGVGGNPHVATLLRAWLISQDPRLLELAERCSAVSLGMNPLNNSFVTGVGTNPVRH
                     PLITDASFGGLPVWPGIPVYGTHPLDDLAWVAEYRLKPAGSNVAAATLPLLRSWFDLP
                     DVAAMNEFTIWQSQAPAIWAFSVLDQ"
BASE COUNT          376 a          700 c          821 g          402 t
ORIGIN      
        1 ctggtccagc acgctgaacg cccagatggc gggagcctgg gattgccaga tggtgaactc
       61 gttcatcgcc gctacgtcgg gcaggtcgaa ccacgagcgc agcaacggca aggtcgcggc
      121 ggcgacattt gagccggcgg gtttcagacg gtactcggcc acccatgcca ggtcatcgag
      181 gggatgggtg ccgtagaccg gtatacccgg ccaaaccgga agccccccga acgaggcgtc
      241 ggtgatcagc gggtggcgaa cagggttggt tcccaccccg gtgacaaacg agttgttcag
      301 cgggttcatg cccagcgaca cggcgctgca gcgttccgca agctccagga ggcgtgggtc
      361 ctgactgatc agccacgctc gcaacagggt cgcaacgtgt gggttgccac cgactcccat
      421 gccccagacc atcggaaccc ccggatcttc gaggcaccac ccgtatcggg tcgtgtccga
      481 tgcgtcggcg atcgcggatg ccacggaagc gaagctggct tcgatgtttg cccgcagagt
      541 ggcgtcggtg cgtgcctggt cgatcaggag gtatctccac ccggcgtcac accattcgaa
      601 gctgtgacag ctgagcgtgc tggcgggcag gtcgcgaaag gggacgtccg cggcgaacga
      661 ttggacgaag cgatcttcgc cggtgaggtc gtacaacgcc gctgcggcgc ccgctcgctg
      721 tgagaggacc ttggcgttgg ccgggtcgcc gtcggtggtc ggcaccggct gggtcgctgc
      781 ccactcccaa gctcgtacgg cgctcgacgc gtactccacg gagcgttggt cgtcgatgcc
      841 ggccaacaac atcgaggcgt cggccgccac cgaggcgtaa acccatgagg accacgggtc
      901 cggtgcgaag gcgtagcggg tgatggtgtc ttgccacgag gcgatgccgg catcggggaa
      961 ccgctcgctc tcaatgccgc cgcgcacggc gccgtcttcg cgctgcagtc gacggaaaag
     1021 gtccacggtc cacagcgcct cgtcgacgag gtcgctgatg ccgttgcccg actcgggcag
     1081 gttcaggctg ccgtccggaa acgcctcggg gtatctgcga gcgagatcga acagcgtgcg
     1141 gaccatgaag aggtgctgtg tgcgccggtc ccagtctccg gcgtcaaagt gaccacccca
     1201 cgcatccgcg accggggtgg tcgttgcctc ggcggtgatg gaatcgaaga gggtctcgtc
     1261 ggtttgttcg gatgcgtcga atccggtcag gttggtttcg aggacggtcg tgtagtcatc
     1321 ggggtggttc gtccgcggcc ggctgaacga cccgtagggc tggttcagtt cgataccgct
     1381 tcgttggtaa tacagcgagt gggcgacggt tgcagccagg cgactccaga tcgcgtcgtc
     1441 gacggcgaag cccggtgagc agccgacctc gtcgacacag acccggtacc gcccggcggt
     1501 cgccacctca tcgaaacgaa gcctccagac agcggcgtcg gtcaggtcgc ctttgccggc
     1561 ctcgctgccg gacggcgccg caactccggt tccgcggaag tgttcgcgcc cctcggaatc
     1621 gacgatcgcg aactcgacgg cggtcggtga actggttgag ccgagcgcgt tgggtattgc
     1681 gtaggtggcg atcttggttc ccgtcgacgt gtagccggct tggttgaccg acaccgcggg
     1741 actgtgcgcg aggtggtcgt cggcgacgag ttcgatcgtg gatcccaccg gagaggtgac
     1801 ggtgtaggtg gcgcctggtt caagtggtgc cgacaacccg aggcgaacct cgtggcgcac
     1861 cgcgaacagt ttcatgtctc cggtggcgtc acgtccgccg tcggtgggag tagagatgcg
     1921 gtcgacgaag ctgagcgtcg gggcgttcgg gccgctgatc ttccatccgg aatcgaacct
     1981 ggtcggctcg acggtggctc cggtgtaggt gtcgccgtcg ttgctgatgc cacggtcgat
     2041 ccggccgatc tcgacgacga cgtccaggat ctggggggtg atcaggccga tcgagcgggg
     2101 aaccggtggg tcggcggtag cggcctcata gccggagtcg atggtgatgc cggcaaccgt
     2161 gaagtcggtc ggagaggctc cgctgccact ggcggtgctg tcgcggaagc gggcgaacac
     2221 ttcctggtag ccctctgagg cgtcgagggt gaccgtggtc gccaaatcgc gccattcccc
     2281 cagtccaacc gagggatcg
//
LOCUS       DAOOMA010000469         2294 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_58417_length_2294_cov_3.004020, whole genome shotgun sequence.
ACCESSION   DAOOMA010000469 DAOOMA010000000
VERSION     DAOOMA010000469.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 2294)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2294)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2294
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58417_length_2294_cov_3.004020"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..790
                     /locus_tag="PLP95_11310"
     CDS             <1..790
                     /locus_tag="PLP95_11310"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: protein motif:HMM:NF013845.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tRNA dimethylallyltransferase"
                     /protein_id="HPB46432.1"
                     /translation="VPHHLIDVLEPSEDSSAGWVQGLARGVVDDLAQRGRRPIFVGGT
                     GLYHRVVVDDMELPASYRELRAELEAANDSAVAEGPEAAARHTAGLLRRLREVDPLTA
                     STCEPNNARRMIRALEVSLGSGRPFSSYGPGMEAYPPSEDLMIGLEVPRAEMLDLLSK
                     RVDAMMAAGFLDEVGALAAEDPPIGVTARQALGYRELLRHVRGEWTLDHAVAETVLRT
                     RQFAVRQHRWFRRDPRIVWHPAPLGDAAKTASLARWIAGTLGAT"
     gene            787..1713
                     /gene="dapF"
                     /locus_tag="PLP95_11315"
     CDS             787..1713
                     /gene="dapF"
                     /locus_tag="PLP95_11315"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HPB46433.1"
                     /translation="MTDVLRLTKAHGLGNDFLVALESDNPQRLDRCVADPSIAVLLCD
                     RRRGIGADGVLYGRTGAAGAAGAAGAAGAAGADLEMVLHNADGSVAEISGNGIRCLAQ
                     AYLRASGRREGDVVIDTAAGRRVLSSAPTERLAVDNLRVDMGSVLAGPPLAPSASAWG
                     AIHVASASVGNPHIVLHVESVDGIDIASEGPRLEADVAGGVNVHFLSVEGPDTIRLVH
                     WERGAGVTEACGSGATVSAFLANQWGLVGAEVSVRMPGGEATIDLREAAEGRIHLTGE
                     AVHVATVEVDLDALVDAANDASTTDAEVANAS"
     gene            1703..>2294
                     /locus_tag="PLP95_11320"
     CDS             1703..>2294
                     /locus_tag="PLP95_11320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020376947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB46434.1"
                     /translation="MHPDQTRPEDQTSPDAHANGFDEERPGQAYPADPANPADPNDSH
                     RGAFGEFGATTSAFIDRSFRERIVLVGVSFPPYDDERTEENLDELARLVDTAGADEVG
                     RIVQRRHSPDPATYIGSGKVEEVKALAEYYDCDTVVFDDELTPAQQYNLERLLGRTAI
                     DRTAVILDIFAQNASSLEGKAQVELALLKYRLPRLRG"
BASE COUNT          344 a          769 c          817 g          364 t
ORIGIN      
        1 ggtgcctcat cacctcatcg acgtgcttga accgagcgag gattcctcgg ccgggtgggt
       61 gcagggcctc gcccgcggag tcgtggacga cttggcccag cgcggccgac gccccatctt
      121 cgtgggcggc acggggctgt atcaccgggt cgtggtggac gacatggagc tgccggcaag
      181 ctatcgcgag ctccgcgccg agctggaggc cgccaacgat tcggcggtcg cggagggccc
      241 cgaggcggcg gcacgtcaca ccgccgggtt gttgcgacga cttcgcgagg tcgatccgct
      301 cacggcgtcg acctgcgagc ccaacaacgc ccggcgcatg atccgggcgc tcgaagtcag
      361 cctcggcagc ggccgtccgt tttcctccta cgggccgggc atggaggcct atccgcccag
      421 cgaggacctg atgatcggcc tcgaggtgcc cagagccgag atgctcgacc tgttgtcgaa
      481 gcgggtcgac gccatgatgg ccgcagggtt tctcgacgag gtcggtgcac tggcggccga
      541 ggacccgccg atcggggtga cggcgcgtca ggcgctcggc taccgcgagt tgttgcgtca
      601 cgtgcgcgga gaatggaccc tcgaccacgc ggtcgctgaa acggtgcttc ggacccgcca
      661 gttcgcggtg cgtcaacatc ggtggttccg gcgcgatccc cgcatcgtgt ggcaccccgc
      721 accccttggc gatgcggcga aaaccgcttc gttggcccgt tggatcgccg gtacgctcgg
      781 tgcgacatga ctgatgtgct tcgcctgacc aaggcccatg gactgggcaa cgacttcctg
      841 gtcgccctcg aatcggacaa ccctcagcgg ctcgaccgtt gtgtggccga cccgtcgatc
      901 gccgtgttgt tgtgcgaccg tcgccggggc atcggcgcgg acggcgtctt gtacggccga
      961 accggagccg ctggagccgc gggtgccgcg ggtgccgccg gagccgcggg tgccgatctc
     1021 gagatggtcc tgcacaacgc ggatggatcg gtcgccgaga tctcgggcaa cggcatccgc
     1081 tgcctagccc aggcgtacct tcgggcctcg ggtcgacgcg agggcgacgt ggtgatcgac
     1141 accgcggcgg ggcgccgggt cctgtcgagt gcgccgaccg aacggctcgc ggtcgacaac
     1201 ctgcgcgtcg acatgggatc ggtgctcgcc gggccgccgt tggcaccgag cgcttcggct
     1261 tggggtgcga ttcacgtcgc gagtgcgagc gtcggcaacc cccacatcgt cttgcacgtc
     1321 gagtcggtgg acggcatcga catcgcctcg gaggggccgc gcctcgaagc cgacgttgcg
     1381 ggcggggtga acgttcactt cctaagcgtc gagggccccg acacgattcg gctggtgcac
     1441 tgggagcgcg gcgccggggt gaccgaggcc tgcggttcgg gggccacggt gtcggcgttc
     1501 ctggcgaatc agtggggcct cgtcggagcg gaggtgtcgg tgcgtatgcc tggcggtgag
     1561 gcgaccatcg atctgcgaga ggcggccgag gggcggatcc atctcaccgg cgaggcggtg
     1621 cacgtcgcca ccgtcgaggt cgacctcgat gcgctcgtcg atgccgcgaa cgatgcgtcg
     1681 accaccgacg ccgaggttgc gaatgcatcc tgaccagacc cgccccgagg accagaccag
     1741 tccagacgct cacgcgaacg gcttcgacga ggagcgtccg ggccaggcgt accccgccga
     1801 cccggcgaat cccgccgatc cgaacgactc gcaccgcggc gcgttcggcg aattcggcgc
     1861 cacgaccagc gcgttcatcg accggtcctt tcgcgagcgc atcgtgctcg tcggcgtgtc
     1921 gtttccgccc tacgacgacg agcgcaccga ggagaatctc gacgaactgg cacgcctggt
     1981 cgacacggca ggtgccgacg aggtcggccg catcgtgcag cggcgccaca gccccgaccc
     2041 ggcgacctac atcgggtcgg gcaaggtcga ggaggtcaag gccctcgccg agtattacga
     2101 ctgcgacacc gtggtgttcg acgatgagct cacccccgcg cagcagtaca acctggaacg
     2161 gctgctcggt cgtactgcca tcgatcgcac cgcggtcatt ctcgacatct tcgcccagaa
     2221 cgcgtcgagc ctcgagggta aggcccaggt cgagttggcg ttgttgaagt accgcctgcc
     2281 gcggctgcgt ggca
//
LOCUS       DAOOMA010000470         2283 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_58802_length_2283_cov_3.271095, whole genome shotgun sequence.
ACCESSION   DAOOMA010000470 DAOOMA010000000
VERSION     DAOOMA010000470.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 2283)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2283)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2283
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58802_length_2283_cov_3.271095"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1850)
                     /locus_tag="PLP95_11325"
     CDS             complement(<1..1850)
                     /locus_tag="PLP95_11325"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HPB46435.1"
                     /translation="MTRVLPWRRNQQPPIEALAPLIEQYRAAHPRGHTDKIQSAYEVA
                     AIAHEGQKRKSGEPYITHPLAVATIVASYGLDEDTICAALLHDSVEDTDVSLDDLAEQ
                     FGPEVARLVDGVTKLDRIHFDSKQAQQAAAIRKMLVAMADDIRVLVIKLSDRLHNMRT
                     IAALPADKQNKTANETLAVYAPLAHRLGMGELKQQLEDLCFASQHPKRYAEIDHMVAT
                     RAPERELYLTQVEEELRQRLALVQIQADVTSRSKHLSSIYDKMVLRGKEFDDIYDLVG
                     LRVIVNSEKDCYAALGAIHSAWMPVVGRFKDFIASPKFNLYQSLHTTVVGPQGKPLEV
                     QIRTREMHQRAESGLAAHFVYKEGAGAPPEDLPWLNRMVDWQRELLDPEEFMANLKTD
                     LEQDEVYVFTPKGDVHTLAKGSTPVDFAYQIHTEVGHRCIGARVGGRLVTLDHKLQSG
                     DTVEIFTAKVDGAGPSLDWLEFVATRSAQQKIKQWFNRERREDEVEAGRELLAKSLRA
                     EPPAVRRLATDETLDALAPQFGVASFEALLRAFVEGRIDPADVVAKLSTEKRGVSEGQ
                     TEVISTGAVSSRSKRRHAVGVHVEGHDDELVRIARCCTPVPGDRIIGFLTR"
     gene            complement(1890..>2283)
                     /locus_tag="PLP95_11330"
     CDS             complement(1890..>2283)
                     /locus_tag="PLP95_11330"
                     /inference="COORDINATES: protein motif:HMM:NF012383.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HPB46436.1"
                     /translation="PREVLCVMAHEMGHYVLGHVVRSILLSFVVTLAGLYFVHRAAGR
                     VIAWFRGRLGFDELRDVASVPLVLLGQRVLLVDDVLATGGTAGAAVALLRSVGATVVA
                     TSFLVELVGLGGRSRVPDVAVSSIVTYD"
BASE COUNT          383 a          771 c          738 g          391 t
ORIGIN      
        1 cgggtcagga agccgatgat gcgatcgcca ggaaccgggg tgcagcagcg ggcgatgcgc
       61 accagttcgt cgtcgtgacc ctcgacgtgt acccccacgg catggcggcg tttgctgcgc
      121 gaggacaccg ccccggtgga gatgacctcg gtctgtccct ccgacacccc gcgcttttcg
      181 gtcgagagtt tcgcgacgac gtcggccggg tcgatccggc cctcgacgaa ggcacgcaac
      241 agcgcctcga aggatgcgac cccgaactgg ggggccaacg cgtcgagggt ctcgtcggtg
      301 gcgaggcggc gaaccgccgg cggctccgct cgaagcgact tcgccaacag ctcacggccg
      361 gcctcgacct cgtcctcacg gcgctcgcgg ttgaaccact gcttgatctt ctgctgtgcc
      421 gaccgggtgg ccacgaactc gagccaatcg agcgacgggc cggcgccgtc caccttcgcg
      481 gtgaagatct cgacggtgtc gcccgactgc agcttgtggt cgagggtgac gagccgaccc
      541 ccaacccgcg cgccgataca ccggtgcccg acctcggtgt ggatctggta ggcgaaatcc
      601 accggggtcg aacccttggc gagggtgtgg acgtcgcctt tcggcgtgaa gacgtagacc
      661 tcgtcctgtt cgaggtcggt cttcaggttc gccatgaact cctccgggtc gagcagctcg
      721 cgctgccagt cgaccatccg gttgagccag ggcagatcct ccggcggcgc gcccgcaccc
      781 tccttgtaga cgaaatgcgc ggcgaggcct gactcggcgc gctggtgcat ctcccgggta
      841 cggatctgca cctcgagggg cttgccctgc ggaccgacga cggtggtgtg cagcgactgg
      901 tacaggttga acttcggcga tgcgatgaag tctttgaacc tccccaccac cggcatccac
      961 gccgagtgaa tcgcgccgag cgcggcgtag cagtccttct cggagttcac gatgacccgc
     1021 aaccccacca ggtcgtagat gtcgtcgaac tccttgccgc gaagcaccat cttgtcgtag
     1081 atgctcgaca ggtgcttgct gcgcgacgtg acgtcggcct gaatctgcac gagcgccagc
     1141 cgttggcgca gctcctcttc cacctgggtg agatagagct cgcgctccgg cgcacgggtg
     1201 gcgaccatgt gatcgatctc ggcgtaacgc ttcgggtgct gtgacgcgaa acacaggtcc
     1261 tcgagctgct gtttcagctc acccatcccg aggcgatgcg ccagcggggc gtacaccgcg
     1321 agcgtctcgt tcgccgtctt gttctgcttg tccgccggaa gcgccgcaat cgttcgcatg
     1381 ttgtgaaggc gatcggacaa cttgatgacc agcacccgga tgtcgtcggc catcgccacg
     1441 agcattttgc gaatcgccgc cgcctgttgg gcctgcttgg aatcgaaatg gattcgatcg
     1501 agcttggtca cgccgtccac gaggcgcgcg acctccggac cgaactgctc cgcaaggtcg
     1561 tcgaggctca cgtcggtgtc ctcgaccgaa tcgtgcaaca gcgccgcaca gatcgtgtcc
     1621 tcgtcgaggc cgtaggaggc gacgatcgtc gcgaccgcga gcgggtgggt gatgtagggc
     1681 tcccccgact ttcgcttctg cccctcatgc gcgatcgccg ccacctcata ggcggactgg
     1741 atcttgtcgg tgtggccccg cggatgagcg gcgcggtact gctcgatcag tggtgcgaga
     1801 gcttcgatcg gtggctgctg attgcgcctc catggaagaa ctcgagtcat cgtcgccatc
     1861 gcgtcgctcc ggggtccgct cggatggggt cagtcgtagg tcacgatcga cgatacggca
     1921 acatcgggaa ctcgcgacct cccaccgagg ccaaccaatt ccacgaggaa gctcgtggcg
     1981 acgaccgtcg ctccgacact gcgtaacaac gccaccgcag cgccggctgt tcccccggtg
     2041 gccaaaacgt cgtcgaccaa caggacccgt tggcccagca ggaccagcgg gaccgacgcc
     2101 acgtccctca gctcgtcgaa cccgagccgc ccccggaacc acgcgatcac ccggcccgcc
     2161 gcgcgatgca cgaagtacag cccggccagg gtgacgacga acgacaggag gatcgatcgg
     2221 acgacgtggc ccaggacgta gtggcccatc tcgtgggcca tgacgcacag cacctcgcgc
     2281 ggg
//
LOCUS       DAOOMA010000471         2280 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_58898_length_2280_cov_5.053483, whole genome shotgun sequence.
ACCESSION   DAOOMA010000471 DAOOMA010000000
VERSION     DAOOMA010000471.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 2280)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2280)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2280
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58898_length_2280_cov_5.053483"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(131..865)
                     /locus_tag="PLP95_11335"
     CDS             complement(131..865)
                     /locus_tag="PLP95_11335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012224214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPB46437.1"
                     /translation="MSDVANLIELQGIRAAYGNIEVLHGIDLEIPAGVVVALLGPNGA
                     GKTTTLNVCCGLVTPTAGKVIVAGRDVTGVAPEELARRGLITIPEGRGVFPNLTVREN
                     LQMASYAGRPIDEIEEMTYAQFPRLGERRSQLVGTMSGGEQQMLAMARGLASNPAVLL
                     LDELSMGLAPLVVEELYAIVADIARTGVSILVVEQFARTVLGIADLAAIMVNGSIRTV
                     GPPAEVEEELSAAYLGGDHGTTGGAR"
     gene            complement(862..1626)
                     /locus_tag="PLP95_11340"
     CDS             complement(862..1626)
                     /locus_tag="PLP95_11340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020378368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPB46438.1"
                     /translation="MALLETRGLTVRFGGNLAVSDVNLAVESGRVTGLIGPNGAGKTT
                     TFNMISGVLTPTAGRVVLDGNDVSKVGTHKRARLGIGRTFQRLEVFSSLSVRENLLVG
                     CEIRQAWARRTPANRQLLAPEGSDPDAGAEVELILDRLGLAEFADVNVGSLSTGRARL
                     VELGRALAIRPRVLLLDEPASGLDDHETEQFGELLVQLAQSGLAILMVEHDVPLVMRV
                     CTQIYVLDFGQIIAEGTPAEVQANESVLNAYLGSAL"
     gene            complement(1613..>2280)
                     /locus_tag="PLP95_11345"
     CDS             complement(1613..>2280)
                     /locus_tag="PLP95_11345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB46439.1"
                     /translation="VGGALMGGLMLGGFQMFLPRFLAGNVVGIFQFFNVDIKNLLQVA
                     PGIMGISLGRNPDGAAAQIAEAFRPVTERKDAFGLMLGGPVIIWLLARSGTVTGWGFV
                     AMMILFLLAIVPLLPVLFAPIEGGRALPAGVWLIACSAIVGGLNWHELLDSNGTRVVA
                     MIVAALVAAFGAVAIHGKVPKMTEPRPSPDLLGIDRPIMRADVLEAETVLGVSEGDFG
                     GLA"
BASE COUNT          413 a          788 c          706 g          373 t
ORIGIN      
        1 cgacgccttg agcttcaggt cgccgatctc cttgttgaac tgctggacgc gatcgggatt
       61 ctcattcatt ggtggttcct ccaaggctgg gagatcgaag gggtgtgggt tggggtcgtg
      121 ggtccgccgg tcaccgcgca ccgccggttg tgccgtggtc gccgccaagg tacgcagcgg
      181 agagttcctc ttccacctcg gccggcggcc caacggtacg gatggatccg ttgaccatga
      241 tcgccgcaag gtcggcgatg ccgagcacgg tgcgggcgaa ttgttcgacg accagaatcg
      301 acacgccggt gcgcgcgatg tccgcgacga tcgcgtataa ctcctccacc acgagtggag
      361 cgagtcccat cgacaactcg tcgagcagca acaccgccgg attcgacgcg agaccccgcg
      421 ccatcgccag catctgctgt tcgccgccgg acatcgttcc cacgagttgg ctgcgccgct
      481 cgcccagcct cgggaactgt gcgtaggtca tctcctcgat ctcgtcgatc ggtcgaccgg
      541 catagctggc catctgcaga ttctcgcgaa cggtcaggtt gggaaacacc ccccgaccct
      601 cgggaatggt gatgagccca cgccgggcaa gctcctctgg ggcgactccg gtcacgtcac
      661 ggccggcgac gatcaccttg cccgcggtcg gcgttacgag gccacaacag acgttgagcg
      721 tcgtggtctt gcccgccccg ttggggccga gcagcgccac cacgaccccc gccggaatct
      781 cgaggtcgat gccgtgcaac acctcgatgt ttccgtaggc cgcacggatt ccctgcagtt
      841 cgatgaggtt cgcgacatcg ctcacagcgc gctcccgaga taggcgttga gcaccgactc
      901 gttcgcctgg acctccgccg gcgttccctc ggcgatgatc tggccgaaat ccagaacgta
      961 gatctgggtg cacaccctca tcacgagcgg gacgtcgtgt tcgaccatca agatggcgag
     1021 gcccgattgc gcgagttgca ccagcagttc tccgaactgc tcggtctcgt gatcgtcgag
     1081 acccgaggcc ggctcgtcca ggagcagcac ccggggccgg atggccagcg ctcggccgag
     1141 ttcgacgagg cgggcccgac cggtggacaa cgagccgacg ttgacatccg cgaactccgc
     1201 gagtccgagt cgatcgagga tcagttccac ctcggcaccg gcgtcggggt cgctcccctc
     1261 gggggcgagc aattgtcggt tcgccggggt ccggcgcgcc caagcctggc gaatctcaca
     1321 acccaccaac aggttctcgc gaacgctcag cgaagagaac acctcgagcc gctgaaaggt
     1381 ccggccgatc cccaatcgag cgcgcttgtg tgtgcccacc ttcgacacgt catttccgtc
     1441 gagcaccacg cgacccgcag tcggcgtcag aacgccggag atcatgttga acgtcgtggt
     1501 cttgccggcg ccgttcgggc cgatcagccc cgtcacccgg cccgattcca ccgcgaggtt
     1561 gacgtcggac acggccaggt tcccgccgaa acgcacggtc aaccctcgag tctcaagcaa
     1621 ggccaccgaa gtccccctca ctcacgccca acacggtttc agcctcgagc acgtcggccc
     1681 gcatgatcgg ccgatcgatt cccaacaggt cgggcgacgg ccgcggttcg gtcatcttcg
     1741 gaaccttgcc gtggatcgcg accgccccga acgccgcgac gagcgcggcc acgatcatcg
     1801 cgacgacccg ggtgccgttg gaatcgagca gttcgtgcca gttcaggcct ccaacgatcg
     1861 cggaacatgc gatgagccac acccccgcag gaagcgctct tcccccttcg atcggagcga
     1921 acagcacggg caacagcggc acgatcgcca ggaggaacag aatcatcatg gcgacaaacc
     1981 cccatccggt gacggtgcca cttcgggcga gcagccagat gatcaccgga ccgccgagca
     2041 tcaacccgaa cgcgtccttg cgttcggtga ccggccggaa cgcttccgcg atctgtgccg
     2101 ccgcaccgtc cgggttgcga ccgaggctga tgcccatgat gcccggggcg acctgcagca
     2161 ggttcttgat atcgacgttg aagaactgga agatcccgac gacgttgccg gcgaggaacc
     2221 tcggcaggaa catctggaat ccgccgagca tgaggccgcc catgagcgca ccgccgaccg
//
LOCUS       DAOOMA010000472         2274 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_59125_length_2274_cov_4.062190, whole genome shotgun sequence.
ACCESSION   DAOOMA010000472 DAOOMA010000000
VERSION     DAOOMA010000472.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 2274)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2274)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2274
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59125_length_2274_cov_4.062190"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            387..734
                     /locus_tag="PLP95_11350"
     CDS             387..734
                     /locus_tag="PLP95_11350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB46440.1"
                     /translation="MMFATFVALALVVTTGVGIAGWRARRLMADPVELSHRVARLLEL
                     QVRRRVGRQWWAVSPIVDASGRVVRLLAVGGVRVGCALPSRRRPQRVNQALWLAAVVA
                     AVVPARRRRVRRR"
     gene            867..1832
                     /locus_tag="PLP95_11355"
     CDS             867..1832
                     /locus_tag="PLP95_11355"
                     /inference="COORDINATES: protein motif:HMM:NF024874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HPB46441.1"
                     /translation="MNLGSLEPVTRMRRWVRTRGRDADAHAALASRHLPLPPGLTIQW
                     LGTAGYRLDYEGQTLLIDPYLSRVSMRDVVTRAPARPNPARLDEFLGDVTAVAGVLVG
                     HTHFDHAVDAPEIARRFGCSAYGSESLRRLMTLHGLGDQAKVVTPHQRYDLGPFVVSF
                     TPSRHSKLIAGLVVPSDGELTCEHLDGLSSSAYRCGQVWGIRIEVAGITIYHQGSADV
                     VEEELTDGPVDVLLAGIAGRYASPGYFETIIRRLSPGIIVPGHYDNFFEPLGTPIEFS
                     LNVNLEAFVEEIERISRNVELVGLPVVGGASSTDGTGSMFMHKRA"
     gene            1861..>2274
                     /locus_tag="PLP95_11360"
     CDS             1861..>2274
                     /locus_tag="PLP95_11360"
                     /inference="COORDINATES: protein motif:HMM:NF014749.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HPB46442.1"
                     /translation="MVRHFLEIDDLSADELAQVLDLAEVEHPELSLTRRGVALLFEKP
                     SARTRHSMEVATIELGGHPVYVRADEVGLDERESVEDIIKVFAGYHAAIAARVFEHDK
                     LVRASAVSSVPIVNLLSDQSHPIQTLADLLTLRQEF"
BASE COUNT          373 a          752 c          735 g          414 t
ORIGIN      
        1 gacacccacg ctttggtgga caggccttcg acgatgtcgg tcatcccaac agcatccagc
       61 tcgcccacca cgtcgatcat gtgcagatcc aaacgccgtt gcagttcgaa caacaccgca
      121 acatcgtcga tcccggaacc caggttattc agctcggtgt cgaagccctc gaacatatgt
      181 atgactctaa acggaacccc cgacactgaa gcccgagacg ccgagaatcc ccgagaattc
      241 ccgaaaatcg ctggaaatca aagccaggca ccccggccag agcggccaac atgtccgacg
      301 gttgggggcc tcgtgggtac tcgtcgaccc ggtcggcaat ctcgagggtt ggacgggcac
      361 gcagtgtgtg cgtctaacct ctgagcgtga tgttcgcgac gttcgtggcg cttgccttgg
      421 tggtgacgac cggcgtcggt atcgccggat ggcgggcgcg tcgactcatg gccgatcctg
      481 tcgagctgtc tcaccgtgtc gctcgtctgc tcgaactcca ggtgcgccgc agggtcggaa
      541 ggcagtggtg ggctgtctcc ccgatcgtcg acgccagcgg gcgggtcgtt cgactgctcg
      601 ccgtcggggg tgtgcgtgtc gggtgtgcgc tgccgagtcg acggcggcct caacgtgtca
      661 accaggcgct gtggctggcg gcggtggtcg ctgcggtggt tcctgcccgt cgccgccgag
      721 ttcgccgccg ctgagttcgc ccgtcgcagc cgcccgcagc agcaccgccc gcagcacccg
      781 cccgcagcac ccgtcgcagc acccgtcgcc gtcctgcgcc gtcccttccg gagtcggcat
      841 cagtggccgc atgcaagaat cgcatcgtga acctcggatc gcttgagccg gtcactcgaa
      901 tgcgcagatg ggtcaggacc cgtggtcgtg acgccgacgc gcatgcggcg ttggccagcc
      961 gccacctgcc actgccgccg ggcctgacca ttcagtggct gggaaccgcg gggtaccgcc
     1021 tcgactacga gggtcagacg ctgctgatcg acccgtacct gagtcgcgtg tcgatgcgcg
     1081 acgtcgtgac gagggcgccg gcgcggccga atccggcgcg gctcgacgag ttcctggggg
     1141 acgtcacggc cgttgcgggg gtgctcgtcg gtcacacgca cttcgatcat gcggtggatg
     1201 ccccggagat cgcccgacgc ttcggatgct cggcgtacgg atcggaatcg ctgcgccggt
     1261 tgatgaccct gcacgggctt ggcgatcagg cgaaggttgt cacgccgcat cagcgctatg
     1321 accttgggcc gttcgtggtg agcttcactc catcgaggca ctcgaagctc atcgccggcc
     1381 tggtggttcc ttccgacggt gaactcacct gcgagcatct cgatgggctt tcgagctccg
     1441 cctaccggtg cggtcaggtg tggggcatcc gcatcgaggt cgcgggcatc acgatctacc
     1501 accagggaag tgccgacgtg gtcgaggagg aactcaccga cggccccgtc gatgtgctcc
     1561 tcgccggcat cgccggtcgg tatgcgagcc cggggtactt cgagacgatc atcaggcgac
     1621 tgtcgccggg aatcatcgtg cccggtcact acgacaactt cttcgagccg ctcggcacgc
     1681 cgatcgagtt ctcactcaac gtgaatctgg aggcgttcgt cgaggagatc gagcggatca
     1741 gtcgcaacgt cgagttggtg ggcctgcccg tcgtcggagg cgccagcagc accgacggca
     1801 ctggcagcat gttcatgcat aagcgtgcat gaacatgcaa agggtggcta gattgtgcga
     1861 atggtccgcc acttcctcga gatcgacgat ctcagcgccg acgaactcgc ccaagtcctc
     1921 gacctcgccg aggttgaaca ccccgaactc agcctgacgc ggcgcggcgt cgcgttgttg
     1981 ttcgagaagc cctccgcccg tacacgtcat tcgatggagg tcgccacgat cgagctcggc
     2041 ggccatccgg tctacgtgcg cgccgatgag gtcggcctcg acgagcggga gtcggtcgag
     2101 gacatcatca aggtgttcgc cggctaccac gccgccatcg ccgcccgggt cttcgaacac
     2161 gacaagttgg tgcgcgcctc ggcggtgtcc tcggtgccaa tcgtcaacct gttgtccgac
     2221 cagtctcacc cgatccagac cctcgccgac ctgttgacgc ttcgccagga gttc
//
LOCUS       DAOOMA010000473         2272 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_59214_length_2272_cov_3.573748, whole genome shotgun sequence.
ACCESSION   DAOOMA010000473 DAOOMA010000000
VERSION     DAOOMA010000473.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 2272)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2272)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2272
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59214_length_2272_cov_3.573748"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..775)
                     /locus_tag="PLP95_11365"
     CDS             complement(<1..775)
                     /locus_tag="PLP95_11365"
                     /inference="COORDINATES: protein motif:HMM:NF023702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase N-terminal domain-containing
                     protein"
                     /protein_id="HPB46443.1"
                     /translation="MEADSSASRRLPAHLGSEAIGHLTRLLSEWSMLADFCFSDLVLY
                     VRDDDRFRMVRHVRPATSQTIYLTDIVGQTRTPMQRPLIAAAFGTGAIHRGTIDSAWL
                     GETIRVEAIPVNLNGSPIAVLAREFVSAYRPHPGELEQTYETVFGRFTEMIADGTFPF
                     PPEDVEHATVPRVGDGVIVVDADGLVEYSSPNANSTLNRSGVEVISRGSSLRDLGFFN
                     VSMRAALATGRPSAEEREFPGDVTVALRVLPFIRNGVAEG"
     gene            complement(777..>1188)
                     /locus_tag="PLP95_11370"
     CDS             complement(777..>1188)
                     /locus_tag="PLP95_11370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB46444.1"
                     /translation="FVVVSTLEAAPVREAQFFLDLLNERKLHVGAIVLNKVLPEYLLR
                     ADGAAAAKEIAANPDEVIAQLAGAWDHEGHGPRVLAEIGTSFNNYRLVAQREAELRAE
                     LSRHADSVAALDYMSEDITDLRGLLRLGAMMWRN"
     assembly_gap    1189..1198
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1199..1935)
                     /locus_tag="PLP95_11375"
     CDS             complement(<1199..1935)
                     /locus_tag="PLP95_11375"
                     /inference="COORDINATES: protein motif:HMM:NF014433.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsA-related P-loop ATPase"
                     /protein_id="HPB46445.1"
                     /translation="MTNSVTLEQLCSAKEIVISTGSGGVGKTTTAASLAATAAAEMNA
                     KVLVLTVDPAKRLANAMGLQSFGNVETRVDPAGFAEAGVTPKGELWAAMLDTKQSWDD
                     LIRRHAPDPKTRDEILKNPLYQNITGKFVQSHDYIAMERLYELHTSGTYDLIVVDTPP
                     TRNAVDFLNAPDRMADFFSSRLLRWLIAPYRSRVVSMASKPFYSIADRVLGSQFLQDV
                     AEFFILFQTMYPGFVSRAEAVTHLLEGS"
     gene            complement(1932..>2272)
                     /locus_tag="PLP95_11380"
     CDS             complement(1932..>2272)
                     /locus_tag="PLP95_11380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="anion-transporting ATPase"
                     /protein_id="HPB46446.1"
                     /translation="LDSETDIDVAAVIVNRVLPELFNRDEEAAFDLLDTPAGRAALSG
                     VVGSGAEAVIDAARLAADLRRSRAVHLERLAGELPNTIPLIMVPYLFTRMHGIRVTRQ
                     VADLLGEEIL"
BASE COUNT          392 a          711 c          775 g          384 t
ORIGIN      
        1 cgccctcggc aacgccgttt cgaatgaatg gcagcactcg caaggcgacg gtgacgtctc
       61 cgggaaactc gcgttcctcg gcgctgggcc gtccggtggc cagcgccgct ctcatcgaga
      121 cgttgaaaaa gccgaggtcg cgtaacgacg agccacgact gatgacctcg accccgcttc
      181 ggttgagcgt cgagttggcg ttcggggagg aatactccac gaggccatcc gcgtcgacca
      241 cgatcacccc atcgcccaca cgtggcaccg tcgcatgttc gacgtcctcc ggggggaacg
      301 gaaaggtccc gtcggcgatc atctcggtga atctgccgaa taccgtctcg taggtttgtt
      361 ccaactcacc aggatggggg cgatatgccg agacgaactc gcgggccagc accgcgatgg
      421 gcgagccgtt gaggttcacc gggatggcct cgacgcgaat cgtttcgccc agccacgccg
      481 agtcgatggt gccgcggtgg atggcgcccg tgccgaaggc cgcagcgatc agcgggcgct
      541 gcatcggggt tcgcgtctga ccgacgatgt cggtcaggta gatggtctgg ctcgtggcgg
      601 ggcgcacgtg gcggaccatg cggaagcggt cgtcatcgcg cacgtacagg acgaggtcgc
      661 tgaagcagaa gtcagcgagc atcgaccatt cggacaacag ccgggtgagg tgcccgatcg
      721 cctcgctgcc gaggtgggcc gggaggcggc gcgacgccga cgaatcggcc tccacctcag
      781 ttcctccaca tcatcgcgcc gaggcgcaac aacccacgga gatcggtgat gtcctcgctc
      841 atgtagtcga gggccgccac cgaatcggcg tggcgcgaca gctcggcgcg caattcggcc
      901 tcgcgttggg cgacgaggcg gtagttgttg aagctcgtgc cgatctctgc gagcacccgt
      961 gggccgtgtc cttcgtgatc ccacgcgccg gcgagttgtg cgatcacctc gtcgggattc
     1021 gccgcgatct ccttggccgc agcggcgccg tcggcgcgta gcagatactc gggcaacacc
     1081 ttgttgagca cgatcgcgcc gacgtgaagc ttgcgttcgt tcaacaggtc gaggaagaac
     1141 tgtgcctcgc gcaccggcgc cgcctcgagg gtcgacacca cgacgaagnn nnnnnnnnga
     1201 accctcgagc aggtgggtca cggcctcggc tcgcgagacg aaaccgggat acatcgtctg
     1261 gaacaggatg aagaactcgg cgacgtcttg gaggaactgg cttccgagga cgcggtcggc
     1321 gatcgaatag aagggcttcg aggccatcga cacgacccga ctgcgatacg gcgcgatcag
     1381 ccatcgcaac agcctcgaac tgaagaagtc ggccatgcga tccggcgcat tgagaaagtc
     1441 gacggcgttt cgggtgggtg gggtgtcgac gacgatcagg tcataggtgc ccgaggtgtg
     1501 gagttcgtag agccgctcca tggcgatgta atcgtggctc tggacgaact tcccggtgat
     1561 gttctgatac agcgggttct tgaggatctc gtcgcgggtc ttcgggtcgg gcgcgtgtcg
     1621 gcggatgagg tcgtcccagc tctgcttggt gtcgagcatc gccgcccaca gctccccctt
     1681 gggggtgacg ccggcctcgg cgaacccggc cgggtcgacg cgggtctcga cgttgccgaa
     1741 cgactgcagg cccatcgcat tcgcgagccg cttggccgga tcgacggtca gcaccagcac
     1801 cttggcattc atctccgccg ctgcggtcgc cgcgagcgag gccgcggtgg tcgtcttgcc
     1861 cactccaccc gaaccggtcg agataacaat ctccttggcc gagcagagtt gttcgagggt
     1921 cacgctgttg gtcacaggat ctcctccccc agcaggtcgg ccacctgccg ggtgacgcgg
     1981 atgccgtgca tccgggtgaa cagatacgga accatgatga gcggaatcgt gttcggtaac
     2041 tcgccggcga ggcgttcgag gtgcacggcg cggctgcgac gaagatcggc ggcgaggcga
     2101 gctgcgtcga tcaccgcctc ggcccccgaa ccgacgacac cgctgagcgc ggcacggccg
     2161 gcgggcgtgt cgagcaggtc gaaggccgcc tcctcgtcgc ggttgaacag ctccggcaac
     2221 acccggttta cgatcacggc tgcgacgtcg atgtcggtct cggagtcgag cc
//
LOCUS       DAOOMA010000474         2271 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_59266_length_2271_cov_3.021661, whole genome shotgun sequence.
ACCESSION   DAOOMA010000474 DAOOMA010000000
VERSION     DAOOMA010000474.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 2271)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2271)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2271
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59266_length_2271_cov_3.021661"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..783
                     /locus_tag="PLP95_11385"
     CDS             <1..783
                     /locus_tag="PLP95_11385"
                     /inference="COORDINATES: protein motif:HMM:NF019998.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TOBE domain-containing protein"
                     /protein_id="HPB46447.1"
                     /translation="IESPLTARSANRLDAASRSQRVHEAAEILGLSDYLNRKPGELSG
                     GQRQRVALARAIVRRPHVFLMDEPLSNLDAKLRNQTRLELVELWRRLETTFIYVTHDQ
                     VEAMTMGTRIAVMNHGELQQVGAPQAVYDRPANSFVAGFIGSPPMNLLAGISETANSI
                     GVGAGRVGVGAAMNAAPGREVTIGIRPEHVRIGGNDLQATVLSLELLGHERHVIASVD
                     GARWVIRQSSAEAAPEVGSVVGLSVDPTQVHAFDTATTERVN"
     gene            783..1655
                     /locus_tag="PLP95_11390"
     CDS             783..1655
                     /locus_tag="PLP95_11390"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HPB46448.1"
                     /translation="MSVAARSRKVREGLLAYAMLAPLIAVFLAFAYYPFVRIIDFALN
                     QKFTNSDETRFVGVRGIWDTLSSADFLDGLWITVRFMIYTVPAGLILGVVLATVANRR
                     LRGIKIFQTIFSSTVASSVAVSAVVFYTLINKNAGFFRDVEFISLSSQTGALRGVALT
                     SVWQNIGLTFIIVLAGMQAIPDEINEAATLDGYGPIRRFFRITVPMISPTLMFLAVVL
                     VIHALQAYAQIDILTNGGPAGSTETLLFKIAQLQGRTDLQRGASMAIGLFVVTLIVSM
                     GQYLMLNKRVHYEN"
     gene            1645..>2271
                     /locus_tag="PLP95_11395"
     CDS             1645..>2271
                     /locus_tag="PLP95_11395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HPB46449.1"
                     /translation="MRTEQRTTGPRKAGSRTTDPSQAGTSTLSKIGWYVLLSAISVVV
                     LFPIYMTLVRAVSSGASTLFAKSPSLTPVDPDWGVFTKAFNTLGMGKPMWQSLVVTSI
                     IVVAQTITSVLAAYAFAFLEFPLKKLMFGFVIATMLLPIEVTLIANVQTMFDLDLISV
                     NNSTYLRTLAALTLPFLASALGVFLIRQGFLGIPRDLLDAARLDGYSDL"
BASE COUNT          386 a          789 c          666 g          430 t
ORIGIN      
        1 atcgagtcgc cgctcaccgc gcgctcggca aatcgtctcg acgcggcctc gcgctcccag
       61 cgggtgcacg aggccgccga gatcctcggc ctgagcgact atctcaaccg caagcccggc
      121 gaactctccg gcgggcagcg ccagcgcgtc gcactcgctc gagcgatcgt gcgacgcccg
      181 cacgtgttcc tcatggacga gccgctgtcg aacctcgacg cgaagctacg aaaccagacg
      241 cgcctcgaac tggtcgagtt gtggcgacgc ctcgagacca ccttcatcta cgtcacccac
      301 gatcaggtcg aggcgatgac gatgggcacc cgcatcgcgg tgatgaacca tggcgaactc
      361 caacaggtcg gggcacccca ggccgtctat gaccgcccgg ccaacagctt cgtcgccggg
      421 ttcatcggca gcccgcccat gaacctgctc gccggcatca gcgaaaccgc caactcgatc
      481 ggggtcggag ccggccgggt cggagtcggc gccgccatga acgctgcgcc ggggcgcgag
      541 gtgaccatcg ggatacgtcc cgaacacgtt cgcatcggcg gcaacgacct gcaggcaacc
      601 gtgttgagtc tcgaactgct cggccacgaa cgccatgtga tcgcctcggt cgacggggca
      661 cgctgggtga tacgccagag ttcggccgaa gccgcccccg aggtgggctc ggtcgtcggg
      721 ctcagcgtcg atccgacaca ggtccacgcc ttcgacacgg ccaccaccga gcgggtgaac
      781 tgatgagcgt cgccgcacgg tcccgcaagg tccgcgaggg cctgctcgcc tatgcgatgc
      841 tggctccgtt gatcgcggtc ttcttagcgt tcgcctacta ccccttcgtg cgcatcatcg
      901 acttcgcact gaatcagaag ttcaccaact ccgacgagac ccgctttgtc ggggtgcgcg
      961 gcatctggga cacgttgagt tcggcggact tcctcgacgg tctgtggatc accgttcgct
     1021 tcatgatcta cacggtgccc gccggcctca tcctcggtgt ggtgctggcc accgtcgcca
     1081 accgcaggct gcgcggcatc aagatcttcc agacgatctt cagttcgacg gtcgcctcgt
     1141 cggtcgcggt gtcggcggtg gtcttctaca cgctgatcaa caagaacgcc ggcttcttcc
     1201 gagacgtcga attcatcagc ctctcgagcc agaccggcgc gttgcgcggc gtcgcgctga
     1261 cctcggtgtg gcaaaacatc ggcctcacct tcatcatcgt cctcgccgga atgcaggcga
     1321 ttcccgacga gatcaacgag gcggccaccc tcgacgggta cggcccgatc cgacgattct
     1381 tccgcatcac cgtgccgatg atctccccca cgctcatgtt ccttgcggtc gtgttggtca
     1441 tccacgcgtt gcaggcgtat gcccagatcg acatcctcac caacggcggc ccggccggct
     1501 cgaccgaaac cctgttgttc aagatcgccc agctacaggg gcgcaccgac cttcaacgcg
     1561 gtgcgtcgat ggccattggg ctcttcgtgg tcacactcat cgtgtcgatg ggccagtacc
     1621 tcatgctcaa caagcgggtg cactatgaga actgagcaga ggacaactgg gccgaggaaa
     1681 gccgggtcga ggaccactga tccgagccag gccggcacct cgacgctgtc caagatcggc
     1741 tggtacgtcc tgctgagcgc catctcggtg gtggtgctct ttcccattta catgacgctg
     1801 gtccgagcgg tgagttcggg cgcgtcgacc ttgttcgcca agtccccgtc gctcaccccg
     1861 gtcgaccccg attggggggt cttcaccaag gccttcaaca cactcggtat gggtaaaccg
     1921 atgtggcaga gcctggtggt cacctccatc atcgtcgtcg cccagacgat cacctcggtg
     1981 ctcgcggcct acgcgttcgc ctttctcgag ttcccgctga agaagctcat gttcggtttc
     2041 gtcatcgcca cgatgctgct gccgattgag gtcaccctca tcgcaaacgt tcagacgatg
     2101 ttcgacctcg acctcatcag cgtcaacaac agcacttatc tacgcaccct cgcggcgctt
     2161 acgcttccat tcctcgcctc cgcgctcggg gtgttcctca tccgccaggg gtttctcggg
     2221 atcccccgag acctgctcga tgcggcgcgc ctcgacggct attccgatct g
//
LOCUS       DAOOMA010000475         2265 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_59487_length_2265_cov_3.351584, whole genome shotgun sequence.
ACCESSION   DAOOMA010000475 DAOOMA010000000
VERSION     DAOOMA010000475.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 2265)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2265)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2265
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59487_length_2265_cov_3.351584"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..481
                     /locus_tag="PLP95_11400"
     CDS             <1..481
                     /locus_tag="PLP95_11400"
                     /inference="COORDINATES: protein motif:HMM:NF019614.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1697 domain-containing protein"
                     /protein_id="HPB46450.1"
                     /translation="MKMADLKAAVVAAGLGDVATLQVAGNIVFDDSGHDSAYWAQLVH
                     DAVLSQFGHDLAVLVRTHRQLVDLVARHRFAGTQEGKWLMTVVLDAQPTPERIASIDP
                     TFGSPDQFVIDGAEIFIRYDGGVAGSKLQTAAFERQLGVVGTARNANTLAKLVEMTA"
     gene            complement(516..1607)
                     /locus_tag="PLP95_11405"
     CDS             complement(516..1607)
                     /locus_tag="PLP95_11405"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012231504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HPB46451.1"
                     /translation="MLVADLPTPALVVDLASLNHNIDAMAKIRPGPSVRSHVKAHKST
                     RLARYAAERSASHSACCATLRELSGMIRAGLGADLLLANETLDVAGLTAVVQAAADGD
                     AQITVAIDSVQTLDVAAAARRRGPLDVLIDVNVGMPRCGVAPERAGELAHRARSAGLE
                     VRGVMGYEGHVVGNPDRSWRIEQVALAMQQLRAAHDDVGGDIVSAGGTGTFDLHDAPP
                     DGTALVSEVQAGSYLLMDTSYATLGLPFRQAVSVLSTVISTNRTDGYGVADAGLKAFG
                     MDHGDPTTIGHTTFFCSDEHVTFVANADDSGRGMPTVGDRVHLVPAHVDPTMALHERM
                     YVVAERNEDGTVELDAEVLDTWPIDLRNW"
     gene            1667..>2265
                     /locus_tag="PLP95_11410"
     CDS             1667..>2265
                     /locus_tag="PLP95_11410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB46452.1"
                     /translation="MRGQAAIAGIAETPFYRGSPRLPIELMLDASLEAIADAGLAPSD
                     IDGICAPPGFTSAEELAANLGIEDLGFSAITAVGGASSVAGIRNAAMAVATGVATSVL
                     VVVGWNGYSWMRPRDGVAVPRHGLAMSSAQDVVVDVMVPQGAIGATQFYAPLANDYRR
                     RRGITDDDGAAFAMAMRRHAHRHPLAVMRDTPMTTEDYF"
BASE COUNT          355 a          723 c          797 g          390 t
ORIGIN      
        1 gatgaagatg gcggatctga aggctgctgt cgtggccgcc ggcttgggag acgttgcgac
       61 tctgcaggta gctggcaaca tcgtgttcga cgactccggg cacgattcgg cgtactgggc
      121 tcagttggtc cacgacgcgg tgttgtcgca attcggccac gacctcgccg ttctcgttcg
      181 aactcaccgc cagctcgtcg acttggtggc ccggcatcgg ttcgccggaa cccaggaagg
      241 caagtggttg atgacggtcg tgctcgacgc tcagcccacc cccgagcgga tcgcttcgat
      301 cgacccgacg ttcgggtctc ctgatcaatt cgtcatcgat ggtgccgaaa tcttcatccg
      361 ctacgacggc ggcgtcgccg ggtcgaagct gcagacggcg gcgttcgaac gccaattggg
      421 cgtcgtgggc accgcgcgta acgcgaacac cctcgcgaag cttgtcgaga tgaccgcctg
      481 actcttcggg tgcaggtccg cgttcaccgc ggcgctcacc agttgcgcag gtcgatcggc
      541 caggtgtcga gcacctcggc gtcgagttcg acggtgccgt cctcgttgcg ctcggcgacg
      601 acatacatcc gctcgtgtag ggccatcgtg gggtcgacat gcgccggcac caggtgcacg
      661 cgatcgccga ccgtaggcat ccctcggccg gagtcatccg cgttcgcgac gaacgtgacg
      721 tgctcgtcgg aacaaaagaa ggtcgtgtgg ccgatcgtgg tcggatcacc gtgatccatc
      781 ccgaacgcct tcagccctgc gtctgcgacg ccatatccgt cggtgcgatt ggtcgaaatc
      841 acggtggaga gcaccgacac cgcctggcga aacggcaggc cgagcgtagc gtaggaggtg
      901 tccatgagta ggtacgagcc ggcctggacc tcgctcacca gcgccgtgcc atcgggcggg
      961 gcgtcgtgga ggtcgaaggt tccggttcca cccgcggaca cgatgtcgcc accgacgtcg
     1021 tcatgggcag cacgtagctg ttgcatcgcc aacgcgacct gttcgattcg ccaggatcga
     1081 tcggggttgc cgacgacgtg gccctcatat cccatcacgc ctcggacctc gagaccggcc
     1141 gacctggccc ggtgcgccag ttcgccggcg cgctcgggcg ccaccccaca gcggggcatc
     1201 ccgacgttga cgtcgatgag gacgtcgagc ggtccgcgtc ggcgtgcggc ggcagcgacg
     1261 tcgagcgtct gaaccgagtc gatggcgacg gtgatttggg cgtcgccatc ggcagcggcc
     1321 tgcacgaccg cggtgagccc ggcgacgtcg agggtttcgt tggccagcaa caggtcggcg
     1381 ccgagcccgg cccggatcat ccccgacagt tctcgaaggg tcgcgcaaca cgccgagtgg
     1441 ctggcggatc gttcggctgc gtagcgggcg aggcgggtgg acttgtgcgc cttgacatgc
     1501 gatcgaaccg aggggcccgg gcgaatcttg gccatggcgt cgatgttgtg gttcaacgag
     1561 gccagatcga cgacgagcgc cggggtcggt aggtcggcga cgagcatcgc cgcaccctac
     1621 ccacgccggg cgagtgtggt tgggccgatc ggtaaggttg aggcccgtga ggggacaggc
     1681 cgccatcgcg ggcatcgctg agacgccgtt ctaccgcggg tcgccgcgtc tgccgatcga
     1741 gttgatgctc gacgcgtcgc tcgaagcgat cgctgatgcc gggctggctc cgagtgatat
     1801 cgacggcatc tgcgctccgc cagggttcac gagcgcggag gagctcgctg cgaacctcgg
     1861 catcgaggac ctgggattct ccgcgatcac cgccgtcggg ggcgcctcga gcgtggccgg
     1921 catacgcaac gcagcgatgg cggtggccac gggtgtcgcg acctcggtgc tcgtcgtcgt
     1981 cggatggaac gggtattcgt ggatgcggcc acgcgacggg gtggcggtgc ctcgccatgg
     2041 gctggcgatg agttcggccc aggacgtcgt ggtcgacgtg atggttcccc agggtgccat
     2101 cggcgcgacc cagttctatg cgccactcgc gaatgactac cgccgccgac ggggtatcac
     2161 cgacgacgac ggggctgcgt tcgcgatggc gatgcggcgc cacgcgcacc gtcacccgct
     2221 cgcggtgatg cgtgacacgc cgatgaccac cgaggactac ttcga
//
LOCUS       DAOOMA010000476         2261 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_59664_length_2261_cov_2.862194, whole genome shotgun sequence.
ACCESSION   DAOOMA010000476 DAOOMA010000000
VERSION     DAOOMA010000476.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 2261)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2261)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2261
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59664_length_2261_cov_2.862194"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            25..>2261
                     /gene="mobF"
                     /locus_tag="PLP95_11415"
     CDS             25..>2261
                     /gene="mobF"
                     /locus_tag="PLP95_11415"
                     /inference="COORDINATES: protein motif:HMM:NF024991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MobF family relaxase"
                     /protein_id="HPB46453.1"
                     /translation="MESEAIQVRRGSGDAAYLNDLAARDPAAAEAAKVRVLPGRGVVA
                     AVFRHRTSRAGDPLLHWHTLVANLVEGPDGRWGAFVHPELYRSVRAAGELFQTALRDE
                     LTDRLGLQWRPGRHVPEVAGVPQRLCDVFSKRAAEIDAWLEATGTPNDPQGRQAAVLA
                     TRRGKPEVEGERFDAAWKAAAIEAGWGPAQAEALLSATAGRTVAYDEIWRLVVSDPIS
                     GAAVDHTVTAEDWIEDLGRTLTDHDTTFTRPQLVEGVAARIGEGASVATVDRIVARVL
                     ASPQIVPIADDNTPRWTTTGMLDIEQRFLAAVHDTRNSRTPVDESMVDHVIGHGPMLG
                     ADQDLAVRTITTSPDSVTVLVGPAGTGKTFTLDAVRRVFESAGHYVVGVAPSARAAHE
                     LATDAHIPSATIHRQLGAWDCGLDPPSERTVLVVDEAAMAGIRELERVTRPVIAAGGR
                     VVLVGDHHQLPEITAGGGFAALATDTDVTIADLTVNRRQLNEWERDALAELRDGHVAA
                     AVSAYRDNDRIVVADDRAEMIATAVDLWLDAHQTGAVPVLLAGTNDVVAALNHAVRDR
                     LLSAGLLTAGADGLAVGERLVMRINDYRARTIDGDDTAVLNGQTGTLTAIDDTGLFVR
                     LDHDDTLVRIDNGYLTAGGIDYAYATTAHRAQGGTWDLAIAVGLDGLYREAGYLVMSR
                     GRQSNWLVLTQPELDSLDPDVGRHDSPLRLLVEEADAIDDLLHDRLSASRRKALAITR
                     APHAP"
BASE COUNT          388 a          883 c          691 g          299 t
ORIGIN      
        1 gccgtccgtc aggcggttgc ctggttggag agtgaagcga ttcaagtgcg gcgcgggtcc
       61 ggtgacgccg cctacctgaa cgacctcgcc gcccgcgatc cggctgccgc cgaggccgcc
      121 aaggtccgcg tccttcccgg gcgtggtgtc gtggccgccg tgttccggca tcgcaccagc
      181 cgcgccggcg acccgctgct ccactggcac acgctggtcg cgaacctcgt ggaaggcccc
      241 gacggccgct ggggcgcctt cgtgcatccc gagctctacc gatcggtccg cgccgcaggc
      301 gaactattcc agacagcgct gcgcgacgaa ctgaccgatc gccttggcct gcaatggcga
      361 cccggccgtc acgtccccga ggtagccggc gtcccgcaac gcctgtgcga cgtgttctcg
      421 aagcgggccg ccgagatcga cgcctggctg gaggccaccg ggaccccgaa cgacccgcag
      481 gggcggcagg ctgcggttct tgcgacacga cgaggcaagc cagaggtcga gggcgaacgg
      541 ttcgacgccg catggaaagc tgccgcgatc gaggccggtt ggggaccggc gcaggccgaa
      601 gcgctgttgt cggctaccgc tgggcggacc gtcgcctacg acgagatctg gcgtctcgtg
      661 gtcagtgacc cgatcagcgg cgccgccgtc gaccacaccg tcaccgccga ggactggatc
      721 gaggacctcg gcaggaccct caccgaccac gacaccactt tcacccgccc gcagttggtc
      781 gaaggggtcg ctgcccggat cggtgaaggg gcgtcggtcg caaccgtcga ccggatcgtc
      841 gcccgcgtgc tcgcctcgcc gcagatcgtc ccgatcgccg acgacaacac gccccgatgg
      901 acgaccaccg gcatgctcga catcgagcaa cggttcctcg ccgccgtcca cgacacccgc
      961 aacagtcgca ccccggtgga cgaatcgatg gtcgaccacg tcatcggcca cggcccgatg
     1021 ctgggtgcgg accaagacct cgccgtccga accatcacga cctcgcccga ctcagtcact
     1081 gtgctcgtcg gccccgcggg cacgggcaag accttcacgc tcgacgccgt tcgccgagtg
     1141 ttcgagagcg ctggccacta cgtggtcggc gtcgccccat ctgcccgagc cgcccacgaa
     1201 ctcgcaaccg acgcccacat cccatcggcc accattcacc gccaactcgg cgcatgggac
     1261 tgcggactcg accctcccag cgaacgcacc gtgctcgtcg tcgacgaagc cgcaatggcc
     1321 ggcatccgcg agctcgagcg ggtcacccgc ccagtgatcg ccgcaggcgg acgtgtggtg
     1381 ctcgtcggcg accaccacca gctccccgaa atcaccgcag ggggaggctt cgcagcactc
     1441 gcaaccgaca ccgacgtcac catcgccgat ctcaccgtca accgtcgcca actcaacgaa
     1501 tgggaacgcg acgccctcgc cgaactccgc gatgggcacg tcgccgccgc cgtcagcgcg
     1561 taccgcgaca acgaccgcat cgtcgtcgcc gacgaccgcg ccgagatgat cgccaccgca
     1621 gtcgacctgt ggctcgacgc ccaccaaacc ggagcggtgc cggtgctcct cgccggaacc
     1681 aacgacgtcg tggccgccct caaccacgcg gtacgcgatc gcctactcag cgccggcctc
     1741 ctcaccgctg gtgctgacgg actcgccgta ggggagcgac tcgtcatgcg catcaacgac
     1801 taccgagcgc ggaccatcga cggcgacgac accgcagtcc tcaacggaca gaccggaacc
     1861 cttacggcga tcgacgacac cggactcttc gtacgcctcg accacgacga cacgctcgtc
     1921 cggatcgaca acggctacct cacagcgggc ggcatcgact acgcctacgc caccaccgcc
     1981 caccgagccc agggcggcac ctgggacctc gcgatcgcgg tcggtctcga cgggctctac
     2041 cgcgaagccg gctacctcgt gatgtcacgc ggccgccagt cgaactggct ggtcctcact
     2101 cagcccgagc tcgacagtct cgaccccgac gtcggccgac acgacagccc cctgcgcttg
     2161 ctcgtcgaag aagccgacgc gatcgacgac ctccttcacg accggctcag cgcctcccgc
     2221 cgcaaggcac tcgccatcac ccgggcccca cacgcgcccg a
//
LOCUS       DAOOMA010000477         2261 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_59667_length_2261_cov_2.502720, whole genome shotgun sequence.
ACCESSION   DAOOMA010000477 DAOOMA010000000
VERSION     DAOOMA010000477.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 2261)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2261)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2261
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59667_length_2261_cov_2.502720"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..613
                     /locus_tag="PLP95_11420"
     CDS             <1..613
                     /locus_tag="PLP95_11420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase cyclase
                     subunit"
                     /protein_id="HPB46454.1"
                     /translation="TASSDGRETTLEMVRHVAEEVFIPFTVGGGIRTVDDARRMLRAG
                     ADKVSSNTAAVERPELISDIAAEFGSQCAVVAVDSRSSDQTPSGFEVYVHGGRTPTGI
                     DVLEWVQRIVELGCGEILLTSMDRDGTRDGFDLAVTRAVSDLVNVPVIASGGVGTLQH
                     LVEGVTLGGADAVLAASIFHFGEFTVAEAKAAMSAAGLTVRPA"
     gene            681..1124
                     /locus_tag="PLP95_11425"
     CDS             681..1124
                     /locus_tag="PLP95_11425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012230955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HPB46455.1"
                     /translation="MSDTPDGISADTPDGTSGDSQGAEADTAGIPAAPAGSRPMGRVD
                     KLPIKMLQDRLMVQLDAAEGERRSSGGILIPATAQMGRRLSWAEVKAAGPAVRNVEIG
                     DQVLFDPSDLSEVEVNGDLFIILRERDVHAVAAERLEVGSTGLYL"
     gene            1139..1516
                     /gene="hisI"
                     /locus_tag="PLP95_11430"
     CDS             1139..1516
                     /gene="hisI"
                     /locus_tag="PLP95_11430"
                     /EC_number="3.5.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-AMP cyclohydrolase"
                     /protein_id="HPB46456.1"
                     /translation="MSDSAIAVTPITPTVEALDKVTYNEHGLVAAIVQDIDTGAVLMM
                     AWMNSETLRMSLEQGRTVFWSRSRSEVWRKGDTSGDRQFIREAYYDCDGDTLLFKVVQ
                     EGKGACHTGAYTCFFSSFGEAGA"
     gene            1519..>2261
                     /locus_tag="PLP95_11435"
     CDS             1519..>2261
                     /locus_tag="PLP95_11435"
                     /inference="COORDINATES: protein motif:HMM:NF016593.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HPB46457.1"
                     /translation="MSEDGEIGGVIPGRDEFRAAARRATVVPVRRELLADQVTPVAVF
                     GRLCGVGEGANESGFLLESVGTAGRWNRWSFVGRSPRARLVCRGGEITVTGDLPDGLD
                     LSDGVLAVVEQLLERYRAEEDDTLPPLHSGIVGYLGYDVVREVEHLPGVPNDDRGWPD
                     AVLSLIGDLVALDHWSQRALLISNVYVPEGASDADLDALYDEAVARIDAMAADGTTPL
                     DEPMVSPPSKSDELPAVESSMPPGQYESAV"
BASE COUNT          347 a          720 c          817 g          377 t
ORIGIN      
        1 cacggccagc tcggatgggc gcgagacgac gttggagatg gtccgccacg tggccgagga
       61 ggtgttcatc cccttcaccg tcgggggtgg gatccgcacc gtcgacgacg cccgacgcat
      121 gctgcgcgcc ggggccgaca aggtgtcgtc gaacaccgct gcggtcgagc gccccgagct
      181 gatcagcgac atcgcggcgg agttcggctc tcagtgcgcc gtggtggccg tcgactcgcg
      241 ttcgagcgac cagaccccgt cgggtttcga ggtgtacgtg cacggcggtc gaaccccgac
      301 cggtatcgac gtgctggagt gggtgcaacg catcgtggaa ctgggctgcg gcgagatcct
      361 gttgacctcg atggaccgcg acggcacccg cgacggcttc gaccttgccg tgacgcgggc
      421 ggtgagcgat ctggtgaacg ttccggtgat cgcctcgggc ggtgtgggaa ccctgcagca
      481 tctcgtcgag ggagtgaccc tcggcggggc cgatgcggtg ctggcggcat cgatcttcca
      541 tttcggtgag ttcaccgtcg ccgaggcgaa ggcggcgatg agcgcggccg gcctcacggt
      601 acggccggcg tgacggtgcg gccgcgtgac ggtgcggccg gctcggtaga ttgttgaccc
      661 gacctcgaga aagaccctgc gtgagcgaca cccccgacgg catctcagcc gacacccccg
      721 atggcacatc tggcgacagc caaggcgccg aggccgacac cgctggcatt ccggcagccc
      781 ccgccggctc gcgcccgatg gggcgcgtcg acaagctgcc catcaagatg cttcaagacc
      841 gcctgatggt gcagctcgac gcggcggagg gcgaacgccg aagctcgggt ggaatcctga
      901 ttccggccac ggcgcagatg ggccggcgct tgagctgggc ggaggtgaag gctgccggtc
      961 cggcggttcg caacgtcgag atcggcgatc aggtgctgtt cgatccgtcc gatctcagtg
     1021 aggtcgaggt gaacggcgac ctgttcatca tccttcgcga gcgcgacgtc cacgccgtcg
     1081 ctgcggaacg gctcgaggtc ggcagcaccg gcctgtattt gtgatcgaga ctggaaccat
     1141 gagcgactca gctattgccg tcacccctat caccccgacc gttgaggcgc tcgacaaggt
     1201 gacctataac gaacacggcc tcgtggccgc catcgtgcag gacatcgaca ccggcgcggt
     1261 gctcatgatg gcctggatga actccgaaac gctgcgcatg agcctcgaac agggccgcac
     1321 cgtgttctgg tcgcggagcc gttccgaggt gtggcgaaag ggcgacacct ccggcgatcg
     1381 tcaattcatc cgtgaggcct actacgactg cgacggcgac acgctgttgt tcaaggtcgt
     1441 gcaggagggc aagggtgcgt gtcacaccgg cgcgtacacc tgcttctttt cctcatttgg
     1501 cgaggcgggg gcctgaccat gtcggaggat ggcgagatcg gcggggtgat ccccggccgc
     1561 gacgagttcc gagccgctgc ccggcgtgcc accgtggtgc cggtgcggcg cgagctgctc
     1621 gccgatcagg tcaccccggt agcggtgttc ggtcgtttgt gcggcgtggg tgagggggcg
     1681 aacgagtccg ggttcctcct cgagtcggtc ggcacagccg gtcggtggaa ccgttggtcc
     1741 ttcgtcggcc ggtcaccgcg ggcgcgcctg gtgtgccgcg gtggcgagat caccgtgacc
     1801 ggcgatctgc ccgatggcct cgacctgtcc gatggagtct tggcggtcgt ggagcagctg
     1861 ctcgagcggt accgggccga ggaggacgac acgttgccgc cgctgcactc gggcatcgtc
     1921 ggctacctgg gctacgacgt ggttcgtgag gtcgaacacc ttcccggggt gccgaacgac
     1981 gaccgtgggt ggcccgatgc ggtcctgagc ctgatcggcg acctcgtcgc gctcgaccac
     2041 tggagccagc gggcgttgct gatctcgaac gtgtacgtgc ccgagggggc gagcgacgcg
     2101 gacctcgacg cgctctacga cgaggccgtc gcacggatcg acgccatggc ggccgacggg
     2161 accaccccgc tggatgagcc gatggtgtcg ccaccgtcga aatccgacga gttgccagcg
     2221 gtcgagtcct cgatgccccc ggggcagtac gaatccgcgg t
//
LOCUS       DAOOMA010000478         2258 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_59767_length_2258_cov_4.202905, whole genome shotgun sequence.
ACCESSION   DAOOMA010000478 DAOOMA010000000
VERSION     DAOOMA010000478.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 2258)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2258)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2258
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59767_length_2258_cov_4.202905"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..583)
                     /locus_tag="PLP95_11440"
     CDS             complement(<1..583)
                     /locus_tag="PLP95_11440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HPB46458.1"
                     /translation="MTTTESRPVEADGQKTIPRIISVDDHVVEPPHVWETWLPAKYRD
                     RGPKVERRGIGTMKHIGGGAYEQTFDPDGPPADCWVFEDLVYIHKRHVAAVGYSRDEM
                     TMTPMTYDEMRPGCYDPVARIADNDMNHVDASLCFPSFPRFCGQAFTEAKDRELGEAC
                     VYAYNDWMVEEWCGDSDGKLIPLIIVPLWDADLV"
     gene            complement(692..1018)
                     /locus_tag="PLP95_11445"
     CDS             complement(692..1018)
                     /locus_tag="PLP95_11445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB46459.1"
                     /translation="MTTLQHGSDDGFDDPSLREITWNPDAGVEHRISHNAIVGVISGF
                     VFVFVLVGVGSLAVGLKTQAALSLGLFSGFWSGTMLGAMVTANAVYQRASSELHHRES
                     GDPTRY"
     gene            complement(1020..1505)
                     /locus_tag="PLP95_11450"
     CDS             complement(1020..1505)
                     /locus_tag="PLP95_11450"
                     /inference="COORDINATES: protein motif:HMM:NF024854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HPB46460.1"
                     /translation="MTRYETLASHLRGDELEDGDFESLLAFRDGLRQFLNWSEQQAKG
                     AGITAAQHQLLLAVRGHRGAASVSDIAGHLLLRHHSVVELLDRATAADLVERVDDLHD
                     QRVVRVRLTPNGEAVLRRLTAAHFEELSRIGDQLTSLWSRLPSMSPSRDGDREPNESR
                     N"
     gene            1591..1911
                     /locus_tag="PLP95_11455"
     CDS             1591..1911
                     /locus_tag="PLP95_11455"
                     /inference="COORDINATES: protein motif:HMM:NF018595.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HPB46461.1"
                     /translation="MGLEVTGDDQIDPGEGVRVKTHPGLCMGYGNCQRFAPDVYLLDD
                     EGYIAVHRLGVPPELAEQAWRGASACPERAITVIGEPERYWVERRNSAGHSHDHSHHH
                     GSVP"
     gene            1917..>2258
                     /locus_tag="PLP95_11460"
     CDS             1917..>2258
                     /locus_tag="PLP95_11460"
                     /inference="COORDINATES: protein motif:HMM:NF025615.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YwiC-like family protein"
                     /protein_id="HPB46462.1"
                     /translation="MDTAAPQQRSALRSVAMPTEHGGWGITVEPGILGLMIAPSLSGL
                     CLALAAVLAFLARTPLKLALVDRRRNRMLARTRLAWQVAVAELTVLALLAIAAIALAE
                     PWFWWPGLVAAP"
BASE COUNT          400 a          749 c          695 g          414 t
ORIGIN      
        1 agaccaagtc ggcatcccac agcggcacga tgatcaacgg gatcaacttg ccgtcggagt
       61 cgccgcacca ctcctcgacc atccagtcgt tgtaggcgta cacgcacgcc tcgccgagtt
      121 cgcgatcctt cgcttcggtg aaggcctgac cgcaaaagcg ggggaaactg ggaaagcaca
      181 gcgacgcatc gacatggttc atgtcgttgt ccgcgatgcg tgcgacgggg tcgtagcacc
      241 ccggccgcat ctcgtcatag gtcatcggcg tcatcgtcat ctcgtcgcgc gagtagccga
      301 ccgcggcgac gtgacgcttg tggatgtaga cgaggtcttc aaaaacccag caatcggccg
      361 gaggaccgtc ggggtcgaag gtctgttcgt aggcaccgcc accgatgtgt ttcatcgtgc
      421 cgatgccacg tcgttcgacc ttcggaccgc ggtcgcgata cttcgccggc agccaggtct
      481 cccagacgtg gggtggttcg acgacgtggt cgtcgacact gatgattcgt gggatggtct
      541 tttgaccgtc cgcctcgacg ggacgtgatt ccgtggtggt catgccgcta cccctctacg
      601 ttcggatggg caaaatctga cggaccgtca gatttggatt tcagggtagc cgacaacgac
      661 gccaccgcct agtcgaccgc cgacgcgcgg ctcaatatcg ggtcgggtcg ccggactcgc
      721 gatggtgaag ttccgatgag gcccgctgat acacggcgtt cgccgtcacc atcgccccga
      781 gcatcgtgcc ggaccagaat ccgctgaaca gcccgaggct gagcgccgcc tgagttttca
      841 gacccaccgc gaggcttccc acgccgacga gaacgaacac aaacacgaat cctgagatca
      901 ccccgacgat cgcgttgtgg ctgatgcgat gttcaacgcc ggcgtcgggg ttccacgtga
      961 tctcacgcag cgatgggtca tcgaagccgt cgtcgcttcc gtgctgaagg gtcgtcatgt
     1021 cagttcctgc tttcgttagg ttctcggtcg ccgtcccgac tcggcgacat ggacgggagg
     1081 cggctccaca agctcgtgag ttggtcaccg attcgcgaca gttcttcgaa atgcgcggcc
     1141 gtgagtctgc gaagcaccgc ctccccgttc ggagtcaacc gcacccgcac cactcgctgg
     1201 tcgtgcaggt cgtcgacccg ttcgaccagg tcggcagccg tcgcccggtc gagcagttcc
     1261 accacgctgt ggtggcgcaa caacaggtgg cccgcgatgt ccgacacgct cgccgccccg
     1321 cgatgcccgc gcaccgccaa caacagctgg tgctgcgcag cggtgattcc ggcacccttg
     1381 gcctgctgct cgctccagtt caggaactga cggagcccgt cccgaaaggc cagcagcgat
     1441 tcgaaatcac cgtcctcgag ttcgtcgccg cgcaggtgcg atgccagggt ttcatatcgg
     1501 gtcacgatat gatcgtagca cgatatatct acgatgagtg cgcccgagcg acgttgcgca
     1561 cagaggaccg gaatcgtata gtcgctcagt atgggacttg aggtaacagg tgacgaccaa
     1621 atcgaccccg gcgaaggcgt gcgggtgaaa acccaccctg gtctgtgcat gggctatggc
     1681 aactgccaac gatttgcccc cgacgtgtac ctgctcgatg acgagggata catcgcggtg
     1741 catcgcttgg gggttccgcc cgaactcgcc gagcaggctt ggcgaggcgc atcagcatgc
     1801 ccggagcggg ccatcaccgt gatcggcgaa cccgagcgct actgggtaga gcgacgcaac
     1861 tccgctggcc acagccacga ccacagccat caccacgggt cggtaccgtg agcaccgtgg
     1921 ataccgccgc acctcagcaa cgcagcgcgc tgcgttccgt cgccatgccg accgaacacg
     1981 gcggttgggg aatcaccgtc gaaccgggca ttctcgggct gatgatcgcc ccgagcctct
     2041 cggggttgtg cctcgccctc gcagcggtgc tcgccttctt ggcccggacc cccctcaaac
     2101 tggccctcgt cgatcgtcgg cgcaaccgca tgcttgcccg aacccgactg gcttggcagg
     2161 tcgcggtcgc cgaactcacc gttctcgccc tgctcgcgat cgcggccatc gccctcgctg
     2221 agccgtggtt ctggtggcct ggtctggtcg ccgcgcca
//
LOCUS       DAOOMA010000479         2255 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_59909_length_2255_cov_3.160909, whole genome shotgun sequence.
ACCESSION   DAOOMA010000479 DAOOMA010000000
VERSION     DAOOMA010000479.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 2255)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2255)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2255
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59909_length_2255_cov_3.160909"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..896
                     /locus_tag="PLP95_11465"
     CDS             <1..896
                     /locus_tag="PLP95_11465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glutamate--tRNA ligase family protein"
                     /protein_id="HPB46463.1"
                     /translation="FARLNLTHTVLSKRTLKTLVDTGAVQGWDDARMPTVRGLRRRGY
                     PAEAIRAFTDHISVAKTNSVVEIELLESFVRTHHNTHALRRMAVVEPLKLTITNWPEG
                     HVEWMDVVNNPEDPAAGTRSVPFTGELWIEQEDFMADPPPKYFRLTPGREVRLRNAYF
                     VTATEAVTDDDGNVIEVLATYDPETKGGNATDGRKVKSTMHWVSRPHAVEATVHRYER
                     LFRAAHPGSETGDVLDDLDPNSRVTTSARVEPALGEIAPGEVVQFERLGYFAADLDDP
                     SVFHRTVGLRDEWANIQKQKK"
     gene            999..1835
                     /locus_tag="PLP95_11470"
     CDS             999..1835
                     /locus_tag="PLP95_11470"
                     /inference="COORDINATES: protein motif:HMM:NF014698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TauD/TfdA family dioxygenase"
                     /protein_id="HPB46464.1"
                     /translation="MNDVVTVTPVAGSLGAEISGVDLGAELDSGTVAAIRAAWLEHLV
                     LFFRDQPLTPEQFLGFARTIGEPVEYKFVPGIDGFPEIISVLKEAGETVNFGGIWHSG
                     TYNDEPPMATMLVAREIPPYGGDTMFANMYDAYEALSPAMRELLDPLKAVNSSALADV
                     SKTSEDRYRESHDDDDEVPLYEAVHPVVRTHPETGRKSLYVNVAHTERFDGMTIEESR
                     PLLEFLFQHQVRPEFTCRFVWKPGSIAMWDNRCAMHNPVNDYHGFRRMMHRITLRGEQ
                     PA"
     gene            1949..>2255
                     /locus_tag="PLP95_11475"
     CDS             1949..>2255
                     /locus_tag="PLP95_11475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB46465.1"
                     /translation="MAALSTLVACGGGEAATSPASTARPEATSTTQSRGSGDSVSEAT
                     DAPLAPGAAIQARLADEFQDAQLAAEVFAGLGEEGRQQLIDRVGEDAATSDVLTYRPK
                     "
BASE COUNT          402 a          728 c          737 g          388 t
ORIGIN      
        1 agttcgctcg cctgaacctc acccacacgg tgctgtcgaa gcgcacgctc aagaccctcg
       61 tcgacaccgg cgcggtgcag ggttgggacg acgcccgtat gccgacggtt cgggggctgc
      121 gccgccgcgg ctatccggct gaggcgatcc gggcgttcac cgatcacatc tcggtggcca
      181 agacgaactc ggtcgtcgag atcgaactgc tcgagtcctt cgtgcgcacg caccacaaca
      241 cccatgccct gcgacgcatg gcggtggtcg agccgctcaa gttgaccatc accaactggc
      301 ccgagggtca cgtcgagtgg atggacgtcg tcaacaatcc cgaggacccg gccgcgggca
      361 cccggtcggt tccgttcacg ggtgagttgt ggatcgaaca ggaggacttc atggccgacc
      421 cgccgccgaa gtacttccgc ctcacccccg gccgtgaggt gcgccttcgc aacgcctatt
      481 tcgtgaccgc gaccgaggcc gtcaccgatg acgacggaaa cgtcatcgag gtgctcgcca
      541 cctacgaccc cgagaccaag ggcggcaacg ccaccgatgg ccgcaaggtg aaatcgacga
      601 tgcactgggt gtcgcggcct cacgccgtcg aggcgacggt gcatcgttac gagcgcctgt
      661 tccgagcggc gcatccgggt tctgagaccg gtgatgtgct cgacgacctc gatccgaaca
      721 gccgcgtgac cacctccgca cgtgtcgagc cggcactggg ggagatcgcg cccggggaag
      781 tcgtgcagtt cgaacgactc ggatatttcg cggccgacct cgacgatccg agcgtgttcc
      841 atcgcacggt ggggttgcgc gacgagtggg cgaatatcca gaagcagaag aagtagggcg
      901 cctggccccg cgcctggccc ggcgcgagac ggtgcgagaa ttgcgttctc gttcgcgaga
      961 tcgatattct tcgttcaggt aaccacggga ggccgctgat gaacgacgtc gtgaccgtta
     1021 cgccggtggc gggatcgctc ggcgctgaga tctctggagt tgacctcggc gccgagctgg
     1081 acagcggcac ggtcgccgcc atccgggccg catggctcga gcacttggtg cttttcttcc
     1141 gcgatcagcc gctcaccccg gaacagttcc ttgggttcgc ccgcacgatc ggcgaaccgg
     1201 tcgagtacaa gttcgttccc ggaatcgacg gctttcccga gatcatctcg gtgctcaagg
     1261 aggccggtga aacggtgaat ttcggcggaa tctggcacag cggcacctac aacgacgaac
     1321 cgcccatggc gacaatgctc gttgcccgtg agatcccgcc ctatggcggc gacacgatgt
     1381 tcgccaacat gtacgacgcg tacgaggcgc tgtcaccagc gatgcgcgag ttgctcgatc
     1441 cgttgaaggc ggtgaacagc tcggcgttgg ccgatgtctc caagaccagc gaagaccgct
     1501 accgcgaatc gcacgacgac gatgacgagg ttccgctcta tgaggcggtg cacccggtgg
     1561 tgcgcaccca tccggagacg ggccgcaaat cgctgtatgt caacgtcgcc cacaccgaac
     1621 gcttcgacgg gatgacgatc gaggagagcc gcccgctgtt ggagttcctg ttccaacacc
     1681 aggtgcgtcc cgagttcact tgccggttcg tgtggaagcc gggatcgatc gcgatgtggg
     1741 acaaccgctg cgccatgcac aacccggtga acgactacca cgggttccgc cgcatgatgc
     1801 accgcatcac gctccgcggc gagcagccgg cctaagcccg cctcaatctc gcgttttgta
     1861 ggtactttct gttgctgtgg caacagaaag tacctacaga accgtcttgg ctgggccgac
     1921 cgccggtcgt tgcgtcacgg ccgtggtgat ggctgcgctg tcaacgctgg ttgcgtgtgg
     1981 cggcggtgag gcggcgacat cgccggcctc gactgcccgc cccgaggcga cctcgacgac
     2041 acagagccgt ggttcgggcg attcggtgtc agaggcgacc gatgcgccgc tcgcgcccgg
     2101 cgctgcgatc caggctcggc tcgccgacga gtttcaggac gcccagctcg ccgcggaggt
     2161 gttcgccgga ttaggcgagg aaggccgtca gcagttgatc gaccgtgtcg gcgaggatgc
     2221 agcgacgagc gatgtgttga cctaccgtcc caagc
//
LOCUS       DAOOMA010000480         2254 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_59933_length_2254_cov_3.558436, whole genome shotgun sequence.
ACCESSION   DAOOMA010000480 DAOOMA010000000
VERSION     DAOOMA010000480.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 2254)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2254)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2254
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59933_length_2254_cov_3.558436"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..231)
                     /locus_tag="PLP95_11480"
     CDS             complement(<1..231)
                     /locus_tag="PLP95_11480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB46466.1"
                     /translation="MFRCRSLAVAVFAVWTLFVWAQRLVNIANDDLEGFDLAWSAGRA
                     VVFVAVGIAVAIVAVRPMTESVARSVVAVASGV"
     gene            339..1394
                     /locus_tag="PLP95_11485"
     CDS             339..1394
                     /locus_tag="PLP95_11485"
                     /inference="COORDINATES: protein motif:HMM:NF025597.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4339 domain-containing protein"
                     /protein_id="HPB46467.1"
                     /translation="MTTLPPPDAPAVFWIDPHGQQQGPEPLNTVIERIAFQQIPSTTP
                     VWWQGAANWIAFDSVAEFAGALQQRLQPAAPAPAPTPSADPAATLDPGGTQESASAFA
                     AEPTIEPTIEPAIETTRLFDADATIQDPAASGSSFGSSPVAEPVTTGPGLFESPDLAP
                     VDLDPIVLADPNPVSAFGAPVDAVPVDAVPIIEPLAAAPIAEPATEPVIEAIVEPTSS
                     SADSDLDAVFSELQARAQPFVDEQTRVAALNDTLSAMVRTAAEHLGHQVVAHDESNGY
                     HMFGLRDASGTPFSLAVTQVPYADTVAEAVARPVGVHVDRGGRASAYLYLGDYLDSMD
                     QALLDRHIAAIIHAAGR"
     gene            complement(1469..>2254)
                     /locus_tag="PLP95_11490"
     CDS             complement(1469..>2254)
                     /locus_tag="PLP95_11490"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HPB46468.1"
                     /translation="GSTEAAGEAAGEAAGDEALVALRGATGHPVNGAGRPTDETTAGS
                     VAASSTSQIPAIAPQGTGIERPVPEWVELNQAIYRAFAFVDLCGFTNYTDKNGAHAAT
                     ELLARFRSATRDVTTRRGTRVAKWLGDGVMLVGTSAGPIVATVAELQLRFLNDEFDIH
                     AGVAAGPVLLFEGDDYIGRAVNLAARLCEAAAPGEILAYGIGDSIPEWVRPAKSVTVR
                     AMGIGDVSGVMQLNVAHDAWADLPESERHPALHPAGGAVRDGS"
BASE COUNT          372 a          858 c          683 g          341 t
ORIGIN      
        1 gacgcccgac gcgacggcga ccacgcttcg ggcgaccgac tcggtcatcg ggcgtaccgc
       61 gacgatcgcg acggcgatac cgacggcgac gaagaccacc gcccgacccg ccgaccacgc
      121 caggtcgaac ccttcgagat cgtcgttggc gatgttgacg agccgctggg cccatacgaa
      181 cagcgtccag acggcgaaca cggcgaccgc aagtgatcga caacggaaca tggagggcag
      241 cgtagtgggg cgacgggccg tggcccctac cgcacgtttc gacggttttt ctcacaatca
      301 cttcaaccga tcgtctcact gtgggaaact cgccgaccat gacgacgctt cctccccctg
      361 acgccccagc cgtgttctgg atcgaccccc acgggcaaca acagggaccc gaaccgctca
      421 acaccgtgat cgaacgcatc gcgtttcagc agatcccgtc gacgacaccg gtgtggtggc
      481 agggtgccgc caactggatc gccttcgact ccgttgccga gttcgccgga gcgctgcaac
      541 agcgcctcca accagcggcc ccagcacccg ccccgacccc ctccgccgat ccggcggcga
      601 cgctcgatcc cgggggtacc caagagagcg cctccgcatt cgccgccgaa cccaccatcg
      661 aacccaccat cgaacccgcc atcgagacga cccggttgtt cgacgccgac gcaaccatcc
      721 aggaccccgc cgcttcgggg tcctccttcg ggtcctcacc cgtcgccgag ccggtcacga
      781 ccggaccggg cctgttcgaa tctcccgatc tggcccccgt cgatctcgac ccgatcgtgc
      841 tcgccgaccc caaccccgtc tcagcatttg gggcaccggt cgacgcagtg ccggtcgacg
      901 cagtgccgat catcgaacct ctcgcggcag ccccgatcgc tgagccggcg actgaacctg
      961 tgatcgaagc gatcgttgaa ccgaccagtt cgtcggccga cagcgacctc gacgccgtgt
     1021 tctccgaact gcaggcccgg gcccagccct tcgtcgacga acagacccgt gtcgccgccc
     1081 tgaacgacac cttgtctgcg atggtgcgca ccgccgccga gcacctcggc catcaggtcg
     1141 ttgcccacga cgagtccaac ggctaccaca tgttcggatt gcgcgacgcc agcggcacac
     1201 cgttctccct ggccgtcacc caggttccgt acgctgacac cgtcgcggaa gccgtcgccc
     1261 gccccgtcgg tgtgcacgtc gaccgtggcg gtcgcgcctc ggcgtatctc tacctcggcg
     1321 actacctcga ctcgatggat caggcgctcc tcgaccgcca catcgccgcg atcatccacg
     1381 cggctggccg ctgagcagca cggtgcggtg cgctgcaggt cgctgagcaa ccggccactg
     1441 gtcggcgctc aggacctcgc cgcggtgatc aggacccgtc gcgcaccgcg ccaccggcgg
     1501 gatgcagcgc agggtggcgc tccgattccg gcaggtcggc ccaggcgtcg tgggcgacgt
     1561 tcagttgcat cacccctgac acgtcgccga tgcccatcgc ccgcacggtc accgacttcg
     1621 cgggtcggac ccattcggga atcgaatcgc cgatcccata ggccaagatc tcacccgggg
     1681 ccgccgcctc gcacagcctc gcggcgaggt tgaccgcccg cccgatgtag tcgtcgccct
     1741 cgaacaacag caccggcccc gcagcaacac ctgcgtggat gtcgaactcg tcgttgagga
     1801 accgaagttg caactcggcc acggtggcca cgatcggtcc ggcggaggtc cccaccagca
     1861 tcaccccgtc cccgagccat ttcgccacgc gggtaccgcg gcgggtcgtg acgtcgcggg
     1921 tggcggaacg aaaccgcgcc agcaattcgg tggcggcatg cgcaccgttt ttgtcggtgt
     1981 agttcgtgaa tccacacagg tccacgaagg cgaacgcccg gtagatcgcc tggttcagct
     2041 ctacccactc gggcaccgga cgctcgattc cggtgccttg gggcgcgatg gcgggaatct
     2101 gcgaggttga cgaggcggcc accgatccgg ccgttgtttc gtcggtcggt cggcctgcgc
     2161 cgttcaccgg atggcctgtc gcaccgcgca acgcgacgag cgcctcgtcc ccggcggcct
     2221 ccccggcggc ctccccggcg gcctccgtgg agcc
//
LOCUS       DAOOMA010000481         2252 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_60004_length_2252_cov_3.291306, whole genome shotgun sequence.
ACCESSION   DAOOMA010000481 DAOOMA010000000
VERSION     DAOOMA010000481.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 2252)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2252)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2252
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60004_length_2252_cov_3.291306"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..369
                     /locus_tag="PLP95_11495"
     CDS             <1..369
                     /locus_tag="PLP95_11495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB46469.1"
                     /translation="RRYEGPRLTSAPTDTVMFSGFLDQDVFIVPSLDLVISRNGLPME
                     SRWRWRLFNRLMPAFPEVSAVDPAAPMPRSTDIHIDWEQIADVETLLRRLSTNGGAVP
                     VADAVGSNVPRVLSDTGLTR"
     gene            485..802
                     /gene="rpsJ"
                     /locus_tag="PLP95_11500"
     CDS             485..802
                     /gene="rpsJ"
                     /locus_tag="PLP95_11500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015797866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HPB46470.1"
                     /translation="MAEQKIRIRLKAYDHEIIDQSTKKIVETVVRTQAKIKGPIPLPT
                     EKNRFTVIRSPHKYKDSREHFEMRVHKRLIDILEPTPKTIDSLQRLDLPAGVDIEIKI
                     QNA"
     gene            1056..1715
                     /gene="rplC"
                     /locus_tag="PLP95_11505"
     CDS             1056..1715
                     /gene="rplC"
                     /locus_tag="PLP95_11505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012229063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HPB46471.1"
                     /translation="MATKTIVGEKVGMTQVWDENNNVVPVTVLSVTPVRVVQIKTPET
                     DGYSALQVTYGSKDPRKVTQPVLGHYDKAGVDPGVRLVELRIDDVASYELGQEITVDL
                     MEAGELVDVTAISKGKGFSGAMKRHNFSGVGASHGAHRVHRKPGSVGQCATPSRVFKG
                     KKLPGQYGNEKVTILNLKVVQADAEANLLLVKGAVPGPNGGLVLIRDAVKGGQASKGD
                     N"
     gene            1715..>2252
                     /gene="rplD"
                     /locus_tag="PLP95_11510"
     CDS             1715..>2252
                     /gene="rplD"
                     /locus_tag="PLP95_11510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020379229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HPB46472.1"
                     /translation="MANITLKNPAGSSAGTVELDDAVFGLEPNVPVMHQVVVAQLAAA
                     RAGTQSTKTRSEVRGGGAKPFKQKGTGRARQGSIRAPQWRGGGVALGPKPRKYDQRTP
                     KKMKKLALRSALSDRAADDKVIVVDSWNFDTPSTKSAVAALAALGVEGRALIVLTPTD
                     VNAWKSFRNIDKVHVITIG"
BASE COUNT          440 a          757 c          700 g          355 t
ORIGIN      
        1 cgacgctatg aagggccgcg gctcacctcg gctcccaccg ataccgttat gttttcgggg
       61 ttcttggacc aggacgtgtt catcgtgccg agcctcgatc tggtgatcag tcgaaacggg
      121 ttgccgatgg agagccggtg gcgttggcgc ctgttcaacc gcctgatgcc ggcgtttccc
      181 gaggtgtcgg cggtcgaccc ggcggcgccg atgccgcgtt cgaccgatat ccacatcgac
      241 tgggaacaga tcgccgacgt ggagacgttg ctgcggcgcc tgtcgaccaa cggcggcgcc
      301 gttccggtgg ccgacgcggt cggttcgaac gtgcccaggg tgctttccga caccggtttg
      361 acgcgctgac ccggcgcgcg actagagtga ttcgccgtgc cacgccgccg cgtggcggtt
      421 gatccttggc aattcgaagt gcaacgggcg cccggatggg tgccctcgag aagtgtgaga
      481 gagcatggct gagcagaaga ttcgcatccg tctcaaggcg tacgaccacg agatcatcga
      541 tcagtcgacg aagaagatcg tcgaaaccgt cgtgcggacc caggccaaga tcaagggccc
      601 gattccgctg ccgaccgaaa agaaccgttt cacggtcatc cgctcgccac acaagtacaa
      661 agactcacgc gagcacttcg aaatgcgtgt tcacaagcgg ctcatcgaca tcctcgagcc
      721 cacccccaag accatcgact cgctgcaacg cctcgacctt ccggctggcg tcgacatcga
      781 gatcaagatc cagaacgcgt aggttcccag aacctcgcgc accgggtttg gtgtcggctc
      841 gccgccggca ctaacctcca gacatcgtgt gaccgggcag aacctcaggt tcggctggca
      901 cacaagcgaa atcgacgttc gagcaggccc gcttcggcgg gaaccgatcg gcaaaccaag
      961 agttcgctcc gctagcccgt gctgtgcgga gcgttcgcgt gagggctccg ctgtccgccg
     1021 ttggtgagac atgagcctag gagtgaaacc tcgtcatggc gacgaagaca atcgttggtg
     1081 aaaaggtcgg gatgacccaa gtgtgggacg agaacaacaa cgttgttccg gtcaccgtct
     1141 tgtcggtcac acccgtccga gtagtccaaa tcaaaacccc cgaaaccgac gggtacagcg
     1201 cgctgcaggt cacctacggg tccaaggacc cgcgcaaggt gacccagccg gtgctcggcc
     1261 actacgacaa ggccggcgtc gaccccggtg ttcgcctcgt cgagttgcgc atcgatgacg
     1321 tggcctccta cgagctcggc caggagatca ccgtcgacct gatggaagcc ggcgaactcg
     1381 tcgacgtcac cgccatcagc aagggcaagg gcttttccgg tgcgatgaag cgtcacaact
     1441 tctccggcgt cggcgccagc cacggcgcac accgcgtcca ccgcaagccc ggctcggtcg
     1501 gccagtgcgc caccccgtcg cgcgtcttca agggcaagaa gcttcccggc cagtacggca
     1561 acgagaaggt gacgatcctc aacctgaagg tcgtgcaggc cgacgccgag gcgaacctgt
     1621 tgctggtcaa gggcgccgtg cccggaccca acggtggcct cgtcctcatt cgcgacgccg
     1681 tcaagggcgg ccaggcatcg aagggcgaca actgatggca aacatcaccc tcaagaaccc
     1741 ggccggtagc tccgccggaa cggtcgaact cgacgacgcc gtgttcggtc tcgagccgaa
     1801 cgtgccggtc atgcaccagg tcgtcgtggc ccaactcgcg gccgcccgcg ccggcaccca
     1861 gtccaccaag acccgctccg aggtccgcgg cggtggagcg aagccgttca agcagaaggg
     1921 caccggccgt gcacgccagg gctccatccg cgccccgcag tggcgcggtg gcggtgtcgc
     1981 cctcggcccc aagccccgca agtacgacca gcgcaccccc aagaagatga agaagctcgc
     2041 gctgcgttcg gccctgtccg atcgcgccgc cgacgacaaa gtcatcgtcg tcgacagctg
     2101 gaacttcgac accccgagca ccaagtccgc cgtcgcggcg ctcgcagcgc tcggagtcga
     2161 gggccgcgcc ctgatcgtgt tgaccccgac cgatgtcaac gcgtggaaga gcttccgcaa
     2221 catcgacaag gtgcacgtca tcaccatcgg tg
//
LOCUS       DAOOMA010000482         2251 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_60039_length_2251_cov_3.842896, whole genome shotgun sequence.
ACCESSION   DAOOMA010000482 DAOOMA010000000
VERSION     DAOOMA010000482.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 2251)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2251)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2251
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60039_length_2251_cov_3.842896"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..328)
                     /locus_tag="PLP95_11515"
     CDS             complement(<1..328)
                     /locus_tag="PLP95_11515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB46473.1"
                     /translation="MRSTRPSRRCTESVVALALVASLSLAACSAGDDASTAAVSADGA
                     SGASGESTERYGGLAMVYEADGGVPQLCAFVSADTPQPCTGIDLIDWSWDLVDDEQSD
                     GDQTSGT"
     gene            complement(388..1296)
                     /locus_tag="PLP95_11520"
     CDS             complement(388..1296)
                     /locus_tag="PLP95_11520"
                     /inference="COORDINATES: protein motif:HMM:NF005861.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HPB46474.1"
                     /translation="MSGIVEGRIVIVTGAGRGLGRAHALAFAAEGAKVVVNDIGAEQD
                     GSGGSLSPAQEVVDTIAAAGGEAVVNGDDVSSDEGAQRLIDSTIERFGGLDVVLNNAG
                     IVCDRMFTNATVDEWDAVMAVHLRGHFLPSKYASLYWKNEAKEGRQRAARIINTSSGA
                     GLMGSIAQAAYSAAKGGIASLTLVQAAELGRYGITANALAPSARTRMTEQAFADMMAE
                     VAEGEFDAMDPANVSPALVWLGSEQSSHVTGRMFELEGGKIGIAQGWMHGPTIDKGAK
                     WDPAEVGDAVDKLLAEAVAPTPVYGA"
     gene            1458..2141
                     /locus_tag="PLP95_11525"
     CDS             1458..2141
                     /locus_tag="PLP95_11525"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03619 family F420-dependent LLM class
                     oxidoreductase"
                     /protein_id="HPB46475.1"
                     /translation="MRYGVTMFATDRSMPVHELAVEAEARGFDSIYIPEHTHIPVSRK
                     TAPPTGDAELKEEYKRTLDPLVALAAAAALTTTIRLGTGVMLPAQREPIVTAKAIATL
                     QNLSNGRFCFGVGFGWNEDEMNSHGVDYRTRRAHMRDHMLTMRSLWRDEVAAYEGTYS
                     HLAESWAWPKPQTEVPVLLGGGAGPKLFAHIAEYGHGWIPIGGAGLTDDIGRFKETVS
                     AAGRDPEAM"
BASE COUNT          382 a          783 c          703 g          383 t
ORIGIN      
        1 cggttcccga ggtctggtcg ccgtcggatt gttcgtcgtc gacgagatcc cacgaccagt
       61 cgatcaggtc gatccccgtg cacggctgag gtgtgtcggc gctgacgaac gcacacaact
      121 gcggcacgcc tccatcagcc tcgtacacca tcgcgagccc gccataacgc tcggtggatt
      181 caccggacgc gcccgatgcg ccatcggccg acacggcggc ggttgacgcg tcatcgccag
      241 cactgcacgc tgcgagcgac aacgaggcga ccagcgccaa cgcgacgacg gattcggtgc
      301 aacgtcggga tggtctggtc gacctcatcg gcccatcatg gcgcacaggg ctcgccggcg
      361 gctcacaatg tgtggaggtg gacgagctca agccccgtag acgggagtcg gagcgaccgc
      421 ctcagcgagc agcttgtcga ccgcatcgcc aacctcggcc ggatcccact tcgcgccctt
      481 atcgatggtc gggccgtgca tccagccctg ggcgatgccg atcttgccac cctcgagctc
      541 gaacatccga ccggtgacgt ggctcgactg ttccgatccc aaccacacga gtgccggcga
      601 cacgttcgcc gggtccatcg cgtcgaattc accctcggcg acctcggcca tcatgtcggc
      661 gaaggcctgc tcggtcatcc gcgtgcgagc cgacggcgcc aacgcgttgg cggtgatgcc
      721 gtagcggcca agctcggcgg cctgcacgag cgtgagcgag gcgatgccac ccttggccgc
      781 cgagtaggcg gcctgggcga tcgaccccat cagcccggcg ccggagctgg tgttgatgat
      841 gcgcgccgca cgctggcggc cttccttcgc ctcgttcttc cagtagagcg acgcatactt
      901 cgaaggcagg aagtggccgc gaaggtgcac cgccatcacc gcgtcccact cgtccacggt
      961 ggcattggtg aacatccggt cacacacgat gccagcattg ttcaacacga catcgagtcc
     1021 gccgaagcgc tcgatcgtgg agtcgatgag tcgctgcgct ccctcgtcgg aggacacgtc
     1081 atcgccgttc accaccgcct caccacccgc cgcagcgatc gtgtcgacga cctcctgtgc
     1141 cggcgagagc gaaccaccgc tgccgtcttg ttcggcaccg atgtcgttga cgacaacctt
     1201 ggcgccctcc gcggcgaagg ccagggcgtg ggcccggcca agtccgcgcc ccgcgccggt
     1261 gacgatgacg attcttcctt cgacgatgcc gctcatgtgt gtccctgtct ctcctcgatg
     1321 cacgaccgct gcggttcttc cagcgtggtg cggtccagtt tcgccggttt ctgacgatcc
     1381 gtcacatttt ctgatcaccg aacaccgctc ccgtgaacag agttggacac ctgtccagtt
     1441 ttcggtagcc tgcgaccatg cgctacgggg tgaccatgtt cgcaaccgac cgttcgatgc
     1501 cggtgcacga gttggcggtc gaggctgagg cccgcgggtt cgactcgatc tacatccccg
     1561 aacacaccca cattccggtc agccgcaaga cggctccgcc gacgggagat gcggaactca
     1621 aggaggagta caaacgcacg ctcgatccac tcgtcgccct agcggcagcc gcggcgttga
     1681 ccacaacgat tcgcctcggc accggcgtga tgctgccggc ccagcgcgaa ccgatcgtca
     1741 ccgccaaggc gatcgccacc ctgcagaacc tgtcgaacgg acgattctgt ttcggcgtgg
     1801 gcttcggctg gaacgaagac gagatgaaca gccacggagt cgactaccgg acccgtcggg
     1861 cacacatgcg cgaccacatg ttgacgatgc gttcgctgtg gcgcgacgag gtggccgcct
     1921 atgagggcac ctactcccac ctcgccgaaa gctgggcctg gcccaagccc cagaccgagg
     1981 tgcccgtgct gttgggcggc ggcgccgggc cgaaactgtt tgcgcacatc gccgagtacg
     2041 gccacggctg gatcccgatc ggcggcgccg ggctcaccga cgacatcggt cgcttcaagg
     2101 aaacggtctc ggcggcgggc cgagatcccg aggcgatgta gatcgttccg ttcgggtcgc
     2161 tgccgagccc gtcgaagctc gaccacttcg agtcgatcgg cgtgaccgaa tgcgtgttcc
     2221 gtatcccgtc tgcaccccgc gacgtggtgc t
//
LOCUS       DAOOMA010000483         2239 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_60494_length_2239_cov_4.432234, whole genome shotgun sequence.
ACCESSION   DAOOMA010000483 DAOOMA010000000
VERSION     DAOOMA010000483.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 2239)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2239)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2239
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60494_length_2239_cov_4.432234"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(199..>2239)
                     /locus_tag="PLP95_11530"
     CDS             complement(199..>2239)
                     /locus_tag="PLP95_11530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB46476.1"
                     /translation="GAGAGLVGEAPDQAGTAAGQPELLAVDEEFSAIESILDRWQEIS
                     EAFDDDDVALKPPVDLGHLRADRDVAVEAVSLAEASVQEHRASEKLVAELERTHDQIF
                     ELDGKSPRIGATKHRRQLEALRETEAKLLEELGFASWSAYVMGVSSNALTSASSPGDD
                     AGQRIVAAARARLVEIDNAIAEAEQDNARRDAALESRQHRGQRRQRLIEDTATLLGAQ
                     QLDHSDAALVDGLLARRSELIAERKAIASDAGAGSGSGAPAGADGDAPGVESGSADSA
                     ASTADSTASADSEELATATENLRWALTTTGAELPDSDLAAEQMGAIAAGWLDAMAGLN
                     DRIGELADERVSLEAEIEEMGARYESLLARVDSGDPSDSGEAPSVAVDHEIPDTADLG
                     VATGFDAAPDLDAPTGLDVPTGFGDPTDLDVPTGFGDPTGLDVGESAGGGDEGFGVVG
                     TFGDSFGDSFSDSFADGAEPLPTEPVPNEPVPNDPLLDGPTDDDPVVIAAKRRLGAAQ
                     ARAQAHRGAAQAYAQAVERRERLAQRTNDVSFSLSEREQRVAAVLEQRAGAHDELQRL
                     ESALRETPQIAHAVGGAGVDDDSASETLEWYVLARLAQQRSVSFVGSVPIVIDDAFAQ
                     WPMHQISEVYTRLERMGDVIQIIMLTDDPAVADWAGGLGESRALVIDPRLSPAHH"
BASE COUNT          362 a          777 c          727 g          373 t
ORIGIN      
        1 ggtccggatt cccgaacccc agatcgatga tgtctttccc ggcgcgccgc tcttcgatct
       61 tgagcgagtc gatgatggtg aacacatacg gcgggaaatt ggtgatgcgc cgaaactcca
      121 taggcctgcg atcctaccgc ccctccccca tggcctcgac ccatatatcc caaccgggcc
      181 gcgccggtgc gaaatcggtc agtggtgagc ggggctgagg cgtggatcga tcacgagtgc
      241 ccggctctcg ccgaggcctc cggcccagtc ggccacggcc ggatcgtcgg tcaacatgat
      301 gatctggatg acgtcgccca tccgctcgag gcgcgtgtac acctcgctga tctgatgcat
      361 cggccactgt gcgaaggcgt cgtcgatcac gatcggcacc gaccccacga acgacaccga
      421 ccgctgctgg gccagccgag cgaggacgta ccactcgagg gtctccgatg ccgaatcgtc
      481 atcgactccg gcccctccca ccgcatgggc gatctgcggg gtctcgcgaa gcgccgactc
      541 caagcgctgc aactcgtcgt gagcacctgc tcgctgctcg agcaccgcgg ccacgcgctg
      601 ctcgcgttcg gacaacgaga agctcacatc gttggtgcgt tgagcgaggc gctcgcgacg
      661 ttcgaccgcc tgtgcgtagg cctgggcggc gccgcgatgg gcctgggctc gcgcctgggc
      721 cgcgccgagg cggcgcttgg cagcgatgac cacggggtcg tcatcggtgg ggccatcgag
      781 caacggatcg ttgggcaccg gctcgttggg caccggctcg gtgggcaacg gctcggcgcc
      841 gtccgcgaac gagtcgctga acgaatcacc aaacgagtcg ccaaaggttc ccacgactcc
      901 gaagccctcg tccccgccgc cggccgactc gccgacatcg aggccggtgg gatcgccgaa
      961 cccggtgggg acatcgaggt cggtgggatc gccgaacccg gtggggacat cgaggccggt
     1021 cggggcatcg aggtctggcg cagcgtcgaa cccggtcgca acgccgaggt cggccgtatc
     1081 ggggatctca tggtccacag caacgctcgg agcctcaccg gagtcgctcg gatcacccga
     1141 gtcgacccga gcgagcaacg attcgtaccg ggcccccatc tcctcgatct cggcttcgag
     1201 gctcacgcgc tcgtcggcga gttcgccgat ccggtcgttc aacccagcca tagcgtcgag
     1261 ccaccctgcg gcgatcgcac ccatctgctc ggcggcaaga tcggagtctg ggagttcggc
     1321 gccggtggtt gtcagggccc accgcagatt ctccgtcgcg gtcgccaact cctccgagtc
     1381 cgccgaggct gtcgaatcgg ctgtcgaggc tgccgaatcg gctgaacccg actccacacc
     1441 gggcgcatcc ccgtcggcgc cggcaggcgc accggaaccg gaaccggcac cggcatcgga
     1501 cgcaatcgcc ttgcgttcgg cgatgagttc cgagcgtcgt gccagtagcc catcgaccag
     1561 cgcggcgtcc gaatgatcaa gctgttgcgc accgaggagc gtggcggtgt cctcgatcag
     1621 acgttggcgg cgttgccccc ggtgctgtcg ggattcgagt gcagcgtcac gacgagcgtt
     1681 gtcctgttcc gcctcggcga ttgcgttgtc gatctcaacg agacgagcgc gggcggcggc
     1741 gacaattcgt tggccggcgt catcccccgg cgaggacgcg gacgtcagcg cgttcgagga
     1801 gacacccatc acgtaggcgc tccagctcgc gaatccgagt tcctcgagca gcttcgcctc
     1861 ggtttcgcgc agcgcctcaa gttgtcgacg gtgcttcgtc gcgccgatcc gaggggactt
     1921 gccatcgagt tcgaagatct ggtcgtgagt ccgttcgagt tcggccacca atttctccga
     1981 cgcgcggtgt tcctgcacgg aggcttcggc gagcgaaacc gcttccaccg cgacgtcacg
     2041 gtcagcacgc agatgaccca gatcgacggg aggcttcagc gcgacgtcat cgtcgtcgaa
     2101 cgcctccgag atctcctgcc accgatccaa gatcgactcg atcgccgaga actcctcgtc
     2161 gaccgcaaga agctccggct gccccgccgc tgttccggct tggtcgggcg cttcacccac
     2221 caggcccgct ccggcgcca
//
LOCUS       DAOOMA010000484         2237 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_60576_length_2237_cov_3.579743, whole genome shotgun sequence.
ACCESSION   DAOOMA010000484 DAOOMA010000000
VERSION     DAOOMA010000484.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 2237)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2237)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2237
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60576_length_2237_cov_3.579743"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..662)
                     /gene="scpB"
                     /locus_tag="PLP95_11535"
     CDS             complement(<1..662)
                     /gene="scpB"
                     /locus_tag="PLP95_11535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HPB46477.1"
                     /translation="MIEHRRAIEAIVMVADEPADPRLLAQLIETSPQQIDDLCSELAA
                     EYDREQRGFELVKIAGGWRFQSRAELAPYVERFVLTGQSARLSAAALETLAIVAYKQP
                     ISRAQVAAIRGVSVDGVMRTLQQRGYIAELARDPGPGQAVLFGTTSLFLERLGVNSLD
                     ELAPLGEFVPNADVVEALEQGLRVPADEAPSPAPDPNAPKWYDAPIEDAPIEDAPGEE
                     VPG"
     gene            complement(666..1445)
                     /locus_tag="PLP95_11540"
     CDS             complement(666..1445)
                     /locus_tag="PLP95_11540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HPB46478.1"
                     /translation="MAIAVQTEVFEGPFDLLLHLILRDQVDLYDINLTRIVDAYLEYM
                     ASMETLDLEVTTEFLLIAATLVDMKARRLLPGLDDGDLDDELALWEERDLLLARLLEC
                     KTFKDAAVVLATMHDHASQNFGRRCGPDERYTELTPDALAGVTPEKLRAAFLRATAPK
                     PEPRIDLFHVAPIRASVVEAVAELVDELPRIGRITFRNLTAAFVDKLEIVVRFLAVLE
                     LFKQGYVDVEQVTSFGDITIVWTGRDDDIDATLELVDAYEG"
     gene            1512..>2237
                     /gene="trpS"
                     /locus_tag="PLP95_11545"
     CDS             1512..>2237
                     /gene="trpS"
                     /locus_tag="PLP95_11545"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HPB46479.1"
                     /translation="MARVFSGIQPTGDIHLGNYLGAVVNWVRDQHVDDSVFCIVDMHA
                     ITIPKDPDELRTKTRELGAMLVACGLDPEVCTLFVQSHVPQHAQLGWIMECTVSFGEL
                     SRMTQFKDKSEGKEFVSAGLFTYPALQAADILLYDTEKVPVGADQKQHIELCRDAAMR
                     FNNRYGDTFTVPVHSTPAAGARVMDLQNPDKKMSKSADSPQGTVLIADDPKAIEKKFK
                     RAVTDSDGEVRYDLDAKPGVSNLL"
BASE COUNT          405 a          748 c          722 g          362 t
ORIGIN      
        1 cccggaacct cctcgcccgg agcgtcctcg atcggggcgt cctcgatcgg ggcgtcgtac
       61 cacttcggcg cgttggggtc gggagccggc gagggtgcct cgtcggccgg aacccgaaga
      121 ccctgttcga gcgcctcgac cacatccgcg ttcggaacga actcgcccag cggcgccaac
      181 tcgtcgagcg aattcacgcc gagccgctcg aggaacaatg aggtcgtgcc gaacagcacc
      241 gcctggccgg ggccggggtc gcgggcgagt tcggcgatgt agccgcgctg ttgcagggtg
      301 cgcatcaccc catccacgct caccccgcga atcgccgcca cctgggcacg gctgatcggc
      361 tgcttgtagg cgacgatcgc cagcgtttcg agcgcggcgg ccgacaaacg tgcggattgc
      421 cccgtcagca cgaaccgctc gacgtagggg gcgagctcgg cacggctctg aaaccgccag
      481 cctcccgcga tcttgaccag ttcaaagcct cgctgttcgc ggtcgtactc ggccgcgagc
      541 tcggagcaca ggtcgtcgat ctgttggggt gaggtctcga tcagttgggc gaggagtcgc
      601 gggtcggcag gttcgtccgc gaccatcacg atggcctcga tcgctcggcg atgttcgatc
      661 acgggttacc cctcgtaggc gtcgacgagc tccagcgtcg cgtcgatgtc gtcgtcgcgg
      721 ccagtccaca cgatggtgat gtcgccgaag ctggtgacct gctcgacgtc gacgtacccc
      781 tgcttgaaca actcgagcac cgccaggaac cgaacgacga tctcgagctt gtcgacgaac
      841 gccgcggtca ggttgcggaa cgtgatgcgg ccgatgcgag gcaactcgtc gaccaactcg
      901 gcgaccgcct cgacgaccga cgcgcgtatc ggcgccacgt ggaacagatc gatgcgcggc
      961 tcgggcttgg gggcggtggc ccgcaggaac gcggcacgca acttctcggg ggtgaccccg
     1021 gcaagggcgt cgggggtcaa ttcggtgtag cgctcgtccg ggccacaacg ccgcccgaag
     1081 ttctgcgacg cgtggtcatg catcgtggcc aacacgaccg cggcgtcttt gaaggtcttg
     1141 cactcgagga gccgcgccaa cagcagatcg cgttcctccc acagggccaa ctcgtcgtcg
     1201 aggtcgccgt cgtcgaggcc gggcaacagc cgccgggcct tcatgtcgac cagcgtcgcg
     1261 gcgatgagca ggaactcggt ggtcacctcg aggtcgaggg tctccatcga ggccatgtac
     1321 tcgaggtagg cgtcgacgat tcgggtgagg ttgatgtcgt acaggtcgac ctggtcgcga
     1381 aggatcaggt gcaacagcag gtcgaagggg ccttcgaaca cttcggtctg cacagcgatc
     1441 gccatgaccg aaacgctaca acggatactc cgcaaatcac aatggcgtga tttcgatagc
     1501 ctgaggcgcc tatggcacgc gtgttttcag gcatccaacc gaccggcgac atccaccttg
     1561 gcaactacct cggcgccgtc gtcaactggg tgcgcgacca gcacgtcgac gattcggtgt
     1621 tctgcatcgt cgacatgcac gcgatcacca tcccgaagga ccccgacgag ctgcgcacca
     1681 agacccgcga gctcggcgcg atgctcgtcg cctgcgggct cgaccccgag gtctgcaccc
     1741 tgttcgtgca gagccacgtc ccccagcacg cccaactcgg ctggatcatg gagtgcaccg
     1801 tcagtttcgg cgagctcagc cgcatgaccc aattcaagga caagtccgag ggcaaggagt
     1861 tcgtctcggc cgggctgttc acctacccgg cgctgcaggc ggccgacatc ttgttgtacg
     1921 acaccgaaaa ggtgccggtg ggtgccgatc agaagcagca catcgagctg tgccgcgatg
     1981 cggccatgcg cttcaacaac cgctacggcg acaccttcac ggtgcccgtg cattccaccc
     2041 ccgccgccgg cgcccgggtg atggacctgc agaaccccga caagaagatg tcgaaatccg
     2101 ccgactcgcc ccagggcacc gtgttgatcg cggacgaccc caaggccatc gaaaagaagt
     2161 tcaagcgtgc ggtgaccgac tccgacggcg aggtccgata cgacctcgac gccaagcccg
     2221 gggtcagcaa cctgctc
//
LOCUS       DAOOMA010000485         2230 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_60844_length_2230_cov_3.549425, whole genome shotgun sequence.
ACCESSION   DAOOMA010000485 DAOOMA010000000
VERSION     DAOOMA010000485.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 2230)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2230)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2230
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60844_length_2230_cov_3.549425"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1569)
                     /locus_tag="PLP95_11550"
     CDS             complement(<1..1569)
                     /locus_tag="PLP95_11550"
                     /inference="COORDINATES: protein motif:HMM:NF012581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HPB46480.1"
                     /translation="MTALLALHLIGGLGIVVAGDRIGRRGFYLALAGPLAVLAWLGTQ
                     IGSILGDDAAAVTQHVEWVPALGLNIDLRLDAFAALMVLLVSGIGVLVFLYATRYFGD
                     GAGVGRLCGLLVLFSGSMLGLVLADNVLFLYTCWELTAVTSFLLIGNNHTETAARAAA
                     LQAILTTALGGLAMLGGFIVIGNEAGTFRLSAILADVPTTTPAQIGLGLVLVGAATKS
                     AQFPFQSWLPGAMAAPTPVSAYLHSATMVKAGVYLVARLSPAVLAVGWFRPTVIAVGL
                     ITMILGGLSALRPTDLKALLANGTISQLGFLFVIFGVGTDEAMLAGSLMLLSHGLFKA
                     ALFMGVGIIDHQAKTRDLAELPVLDGRWRPVRVFMSISAASMAGIPLAFGFVAKETVL
                     EALTGSGIDGGWIVVAGVVVGSALTAAYSIRIIWGTFVWPSRRVAAEVAGGDGATRRA
                     APAPSAAFIAPACVLTAFTLVFGVAPGLLNTFAGSAQASLLLPTGRAAAHVVAHLSLW
                     HGFTAALGLSALALG"
     gene            complement(1741..2193)
                     /locus_tag="PLP95_11555"
     CDS             complement(1741..2193)
                     /locus_tag="PLP95_11555"
                     /inference="COORDINATES: protein motif:HMM:NF016001.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase/quinone reductase family protein"
                     /protein_id="HPB46481.1"
                     /translation="MQYPDRVVAAAAWALENGHRTLSALTGGRWPKRLLGMETLELHT
                     IGRRSGERRSTLLTSPMASDDRVVLVASKGGHSRHPAWYLNLVANPGIELTRSGVTSP
                     WVARTASPEEKASMWPDIVKVYGGYASYQANTERDIPVVICEPASLAS"
BASE COUNT          421 a          766 c          745 g          298 t
ORIGIN      
        1 cccaagggcc aacgcggaga gtccgagcgc cgcggtgaac ccgtgccaca gcgaaaggtg
       61 ggcgacgacg tgggcggccg ctcgaccggt tggcagaagc aggctggctt gggccgaccc
      121 ggcgaaggtg ttgagcagac cgggcgcgac accgaacacg agggtgaagg ccgtcaacac
      181 gcacgcgggg gcgatgaatg cggcgctggg tgccggcgcc gctctcctcg tcgccccgtc
      241 gccaccggcg acctcggccg cgacgcgacg agagggccac acgaaggttc cccaaatgat
      301 gcggatgctg taggcggcgg tgagggcgga tccgaccacc acgccggcca cgacgatcca
      361 gccgccgtcg atcccgctgc cggtcaacgc ttcgagcacc gtttccttcg cgacgaaccc
      421 gaacgccagc ggaatacccg ccatcgacgc ggcggagatc gacatgaaga cccgcacggg
      481 gcgccaccgg ccgtcgagca ccgggagctc cgcgaggtcg cgggtcttcg cctggtggtc
      541 gatgattccg acccccatga acaaggccgc tttgaacaac ccgtggctca gcaacatgag
      601 cgaacccgcc aacatcgcct cgtcggttcc gaccccgaag atcacgaaca ggaatccgag
      661 ctggctgatc gtgccgttcg ccaacagcgc tttgaggtcg gtcgggcgca gcgcactcaa
      721 ccctccgagg atcatcgtga tcagcccgac tgcgatgacc gtcgggcgga accacccgac
      781 ggcgagcacc gccggagaaa gccgggcgac gaggtacacg ccggctttca ccatggtcgc
      841 cgagtgcagg taggcgctca ctggggtcgg cgcggccatg gcgccgggca gccaggactg
      901 gaacgggaac tgagcgctct tggttgcggc gccgaccaac accagaccca gcccgatctg
      961 ggccggggtc gtggtcggca catcggccag aatcgcactc agtcgaaacg ttccagcctc
     1021 gttgccgatg acgatgaatc cacccagcat cgccaacccg ccgagcgcgg tggtgagtat
     1081 cgcttgcaag gccgcagcgc gcgccgcggt ctcggtgtgg ttgttgccga tcagcaggaa
     1141 cgaggtgacg gcggtgagtt cccaacacgt gtaaaggaac aacacgttgt cggcgagcac
     1201 caggccgagc atcgacccgc tgaacaacac gagcagtccg cacaggcgcc ccacccctgc
     1261 gccgtcgccg aagtatctcg tggcgtacag gaacaccagg accccgatgc cggacacgag
     1321 cagaaccatc agcgcggcga atgcatccag gcgcaggtcg atgttcaggc ccaacgcggg
     1381 gacccactcg acgtgttgtg tgaccgccgc cgcatcgtcg ccgagaatcg aaccgatctg
     1441 tgtcccaagc cacgccagca cggcgagcgg gccggccaac gccaggtaga agccgcgccg
     1501 tccgattcgg tcgccggcaa cgacgatgcc gagcccgccg atgaggtgaa gcgccaacag
     1561 cgccgtcatg acggaccgct cccgtgatag aggtcggtgc ggaaagcgac ggccgaaacg
     1621 ggacgtccaa cgccctccgt cgtgccaagg cggagtcgca tggtcgcagc agtttacaca
     1681 tgcggcgcgc ccagttgaat cacgtcgacc cggatccagc cgaacggagg ccggttcgga
     1741 tcagctcgcc aacgacgccg gctcgcagat caccaccgga atatcgcgtt cggtgtttgc
     1801 ctgatacgac gcgtacccgc cgtagacctt cacgatgtcg ggccacatcg acgccttctc
     1861 ctccggcgag gccgtgcggg cgacccatgg cgaggtcacg ccgctgcggg tcagttcgat
     1921 gccggggttc gcgacgaggt tcaggtacca cgccgggtgg cgggagtggc cgcccttgga
     1981 cgcgaccagt acgacacgat catccgaggc catcggactg gtgagcagcg tcgagcgccg
     2041 ttcgcccgag cggcgaccga tcgtgtgcag ctcgagcgtc tccatgccga gcaggcgctt
     2101 gggccaccgg ccaccggtga gcgccgacag cgtgcggtgc ccattttcca acgcccaggc
     2161 tgcggcggcg acgactcggt cgggatactg catcgccaca cgctacccgg cgtccgccga
     2221 ccgatcccgg
//
LOCUS       DAOOMA010000486         2228 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_60930_length_2228_cov_3.976530, whole genome shotgun sequence.
ACCESSION   DAOOMA010000486 DAOOMA010000000
VERSION     DAOOMA010000486.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 2228)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2228)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2228
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60930_length_2228_cov_3.976530"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..260)
                     /locus_tag="PLP95_11560"
     CDS             complement(<1..260)
                     /locus_tag="PLP95_11560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB46482.1"
                     /translation="MFSRLNVRTKLLVMVLPVTMVLIVVAGFGIQARLVDRAEAKQQR
                     EALVVSAQTADLVHELQRERLLAASAGLGDEAWNVQSGWRWM"
     gene            421..1227
                     /locus_tag="PLP95_11565"
     CDS             421..1227
                     /locus_tag="PLP95_11565"
                     /inference="COORDINATES: protein motif:HMM:NF025690.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4388 domain-containing protein"
                     /protein_id="HPB46483.1"
                     /translation="MSADPQTETHPIAAAPAARVAATLQGSLDTLSVRDVLALLAGSR
                     RTGRLVVRPAQPLWLVLHDGRIVHAGASSTMVLPRHLLSIGALTAGDLEQMFSLSNAS
                     VSASLSPAEVLAMLQRAAEDGTLDPEALSTAIFELSVSTVTDLLSVPERDHRGELNTL
                     VFTENDGVAFAPDLTVDLDDVVKAADSDLASLAEVESRIGDLESRVRRVQRLHRGTGS
                     VTLDALDWAALAEIDGRATLAQLGHRLGLGRGRLYRLVESLLERGLVEPA"
     gene            complement(1283..>2228)
                     /locus_tag="PLP95_11570"
     CDS             complement(1283..>2228)
                     /locus_tag="PLP95_11570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glutamate synthase subunit beta"
                     /protein_id="HPB46484.1"
                     /translation="DRVGGLLRYGIPEFKMEKRHLDRRIAQMRAEGTVFLTNHTVGID
                     VTAEHLRSNYDAVVLAVGATQARDLPIPGRDALGVYQAMEYLPSANRGAYGDYEPSES
                     PIHAEGKHVIIIGGGDTGADCLGTAHRQGAASVHQFEIMPRPSDTRPDANPWPTFPMV
                     YKVTSAHEEGGERVYSVNTEEFLKDEEGNLVGLRFVEVEQRFVDGRMTFEKVEGSETV
                     LEADLVFLAMGFTGPENGPLLSQLGVELDERTNVARDDRFATNVDGVFVAGDAGRGQS
                     LIVWAIAEGRAAAAAVDAHLMGSTDLPAPIVPTDRPLV"
BASE COUNT          366 a          771 c          724 g          367 t
ORIGIN      
        1 aacatccacc gccagccaga ctggacgttc caggcttcgt cgcccaggcc ggcactggcg
       61 gcgagaagtc gttcacgttg cagctcatgc acgagatcgg ccgtctgggc agacactacg
      121 agcgcttcac gctgctgttt ggcctcggcc cggtcgacga gtcgggcctg aatgccgaac
      181 ccggcgacca cgatgagcac catggtcacg gggagcacca tgacgagcag tttcgtgcgg
      241 acattgagac gggagaacat ggggcctctc tgacgtggaa caagccgagg gatggcaggc
      301 ggggcacgag tcgtatcgac gcggcaccgc accggcttga ggatcccgtc tcaagtcctc
      361 tcccgggccg acccggcaag ggcgggagga agtgggccgt gtggtcgata cattgacctc
      421 gtgagcgccg acccgcagac cgagacgcac cccatcgccg cggcgcccgc ggcgcgggtc
      481 gccgcaacgt tgcaggggtc gctcgacacc ctgtcggttc gagacgtgct cgccctgttg
      541 gccggctcac ggcgtaccgg ccgtctcgtc gtacgccccg ctcaaccgct gtggctcgtg
      601 ctgcacgacg ggcgcatcgt tcacgcgggg gcctcctcga cgatggtgct tccccggcac
      661 ctgctctcga tcggggcgct gaccgcgggc gaccttgaac agatgttttc actcagcaac
      721 gcctcggtga gcgcgtcgct gtcccctgcc gaggtactcg ccatgttgca gcgggccgcc
      781 gaggacggca ccctcgatcc cgaggcgctc agcaccgcga tcttcgaatt gagcgtctcg
      841 acggtgaccg atctgctctc ggttcccgaa cgcgaccacc gcggcgaact caacacgctc
      901 gtgttcaccg aaaacgacgg agtggccttt gccccggacc tgaccgtcga cctcgacgac
      961 gtggtcaagg ctgcggacag cgacctggca tcgctcgctg aggtcgagag ccgaatcggc
     1021 gacctcgaat cccgggtgcg aagggtgcag cgcctgcatc gcggcaccgg ctcggtcacc
     1081 ctcgatgcgc tcgattgggc cgcgctggcc gagatcgacg ggcgcgccac actcgcccag
     1141 ctcgggcacc gcctcggcct cggccgcggt cggctctacc ggcttgtcga gtcgttgctc
     1201 gaacgcggac tcgtcgagcc cgcctgaccg agcccgcctg aacgagcccg cctgaacggg
     1261 cccggcccga tcgaccccct cgctacacca gcggccgatc ggtcggcacg atcggagcgg
     1321 gaaggtcggt gctacccatg agatgagcgt cgaccgcggc tgccgcagcg cgcccctcgg
     1381 cgatcgccca cacgatcagg ctctgcccac gaccggcgtc gcccgcgacg aatacgccgt
     1441 cgacattggt ggcgaaccga tcgtcgcgtg cgacgttggt gcgctcgtcg agttcgacac
     1501 cgagttggct caacagcggc ccgttttccg gaccggtgaa tcccatggcc aggaacacca
     1561 ggtcggcctc cagaaccgtc tcggacccct cgactttctc gaaggtcatg cgaccatcga
     1621 cgaagcgctg ttcgacctcg acgaaccgca ggccgaccag gttcccctcc tcatccttca
     1681 ggaactcctc ggtattgacc gaatagaccc gctcgccgcc ctcctcgtgg gcagaggtga
     1741 ccttgtagac catcggaaac gtcggccacg ggttggcatc ggggcgggtg tcgctgggtc
     1801 gcggcatgat ctcgaactgg tgcaccgatg cggcgccctg tcgatgtgcc gtgccgaggc
     1861 aatccgcacc cgtgtcgcca ccaccgatga tgatgacgtg cttgccctcg gcgtggatgg
     1921 gtgactccga tggctcgtag tcgccatacg cgccacggtt cgccgaaggg agatactcca
     1981 tggcctgata cacgccgagc gcgtcccgac cggggatcgg gaggtcgcgt gcctgggtgg
     2041 cgcccaccgc caacacgacc gcgtcgtagt tgctgcgaag gtgctcggcg gtcacgtcga
     2101 ttccgacggt gtgattcgtg agaaacacgg tgccctcggc acgcatctgg gcgatacgac
     2161 gatcgaggtg acgcttttcc atcttgaatt cggggatgcc gtagcgcagc agaccaccca
     2221 cgcgatcg
//
LOCUS       DAOOMA010000487         2228 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_60932_length_2228_cov_3.699954, whole genome shotgun sequence.
ACCESSION   DAOOMA010000487 DAOOMA010000000
VERSION     DAOOMA010000487.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 2228)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2228)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2228
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60932_length_2228_cov_3.699954"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(85..1488)
                     /locus_tag="PLP95_11575"
     CDS             complement(85..1488)
                     /locus_tag="PLP95_11575"
                     /inference="COORDINATES: protein motif:HMM:NF017774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HPB46485.1"
                     /translation="MSVELELIRPALELAVEVARSGCTATPPVDPPRALVPFLRFRRI
                     PAKALVAARRVIDGDEEFRARVAAEVVEAEGVEGVEGAVESAVLGRAAVVYLTRPDGW
                     EQEFGDLVEIAERDHAIASETAAAVNRDRSAERLGRQVEELRRTLADSEASRHTEEQR
                     ARSALQRVDAMAQALDGAEASLNAAEAARQRAVRELKATERRLAERIAEVKELSARVE
                     ELEAEVLLQQGDALAADAVASAVQARQSSDALLVETLRERWDALAPALGEIQRLLAVP
                     SGDAASTPAELLKDAQTARSSAGGATGPGNSRSTAEHAGSARAVRRAPRLPARIAPGS
                     AEATRWLLSRPNLTVFVDGYNVTMLGWPELNATEQRSMLERSARRLQARHGCRVVLVF
                     DGDGEGGRDVRTALGSPVRVMFTEASVEADDEILRQLDLGTEPAVVVSNDRRVQTGAQ
                     ERGAVVMSSTEFLTILS"
     gene            complement(1522..1689)
                     /locus_tag="PLP95_11580"
     CDS             complement(1522..1689)
                     /locus_tag="PLP95_11580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB46486.1"
                     /translation="MATRTTRAFHHYSVGGELQIEDVEVLSETIEDVSCRWCATGANV
                     VELGAVTPSDI"
     gene            1874..>2228
                     /locus_tag="PLP95_11585"
     CDS             1874..>2228
                     /locus_tag="PLP95_11585"
                     /inference="COORDINATES: protein motif:HMM:NF013122.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease domain-containing protein"
                     /protein_id="HPB46487.1"
                     /translation="MSPISSPSVAAPRQISFDDLGTPLNEVTFCIIDFETTGTSCDRD
                     TITEIGAVKVHAGAEVARFQTLVNPGRLIPPQVTVLTGIAQAMVTTAPRIEAVLPTLL
                     EFIADCVMAGMARSGS"
BASE COUNT          404 a          816 c          650 g          358 t
ORIGIN      
        1 tcaggtgagg tgagaagatc gaacatgtgt tcagagtatc aggaaccaga acacgtgttc
       61 gaattattgt gtgggtaatt tcacctacga caaaatcgtc aggaactccg ttgagctcat
      121 cacgaccgct ccccgctcct gcgcgccggt ctgaactcgc cggtcgttcg acaccacgac
      181 cgcaggttcc gtcccgaggt cgagttggcg cagaatctcg tcgtccgcct cgaccgaagc
      241 ctcggtgaac atgacccgca ccggcgagcc aagcgccgtg cgcacatcac ggccgccttc
      301 gccatcaccg tcgaagacca acacgactcg acaaccgtgg cgagcctgaa gccggcgtgc
      361 cgaacgctcc aacatcgagc gctgctcggt cgcgttcaac tccggccaac ccaacatcgt
      421 cacgttgtac ccgtcgacaa acaccgtgag gttcggccga gacagcagcc atctggtcgc
      481 ctcagcgctg ccaggcgcga tccgtgcggg cagccgaggc gcccgccgaa ccgcacgcgc
      541 acttccagcg tgctcggccg tcgacctgga gttgccgggc cctgtcgccc caccagcgga
      601 cgaacgcgcg gtctgggcat ctttcaacag ttcggctggc gtcgatgccg cgtcgccaga
      661 cggcaccgcc agcaaccgtt gaatctcacc gagcgccggc gcgagcgcat cccatcgctc
      721 ccgaagggtc tccaccaaca gggcgtcgct gctttgacgc gcctgaaccg ccgacgcgac
      781 cgcgtccgca gccaacgcat cgccctgctg cagcagcact tcggcctcga gctcctcaac
      841 ccgtgcgctc aactccttga cctcagcgat gcgctcggcc aaccgacgct cggtggcttt
      901 caactccctg accgcgcgct ggcgcgccgc ttccgccgcg ttcagcgacg cctccgcccc
      961 atcgagcgcc tgcgccatgg cgtcgacccg ctgtagcgct gatcgggctc gctgctcctc
     1021 ggtgtggcgc gaggcctccg aatcagccaa cgtccggcgg agctcttcga cctgtcgccc
     1081 gaggcgctcc gcacttcgat cacggttcac cgcggcggcg gtttcagacg cgatcgcatg
     1141 gtcgcgctcg gcgatctcga cgagatcgcc gaactcttgc tcccagccgt ccggccgcgt
     1201 caggtaaaca actgcggccc ggcccagcac tgcggactcg actgccccct cgaccccctc
     1261 gaccccctcg gcctcgacga cctccgccgc gacccgcgct cggaactcct cgtcgccatc
     1321 gatcacgcgc cgcgccgcca ccaacgcctt ggcggggata cgccggaagc gaaggaacgg
     1381 gaccaacgct cgcggcggat cgaccggcgg cgtcgcggtg catcccgatc gagccacctc
     1441 gaccgccagc tcaagcgcag ggcgaatgag ctccagttcg acgctcacga cgacccccct
     1501 tccggctccc ccgcatcgga ttcagatatc ggagggtgtt accgcaccga gctccacgac
     1561 gttcgcgccc gttgcacacc agcgacatga cacgtcctcg atcgtctccg acaacacctc
     1621 gacgtcttcg atctgtagct caccgccgac gctgtaatgg tgaaatgcac gggtcgtgcg
     1681 ggttgccaca acgtcgaaac gagtgaggtt cccgcattgc gaacaccggt accgcacacc
     1741 gatatcggga aggcggggtg cccgggttgt gtctgtcgaa tccggcgagg tcacgcgacg
     1801 aatctacccg agtgcggcgc cacgtcccga aatcgttgac aagcgaacac gcgttcacta
     1861 gaacggtcgt tcgatgtcgc cgatcagctc tccgtcagtg gcggcccctc gccagatcag
     1921 cttcgacgat ctcggaacac cactgaacga ggtcacgttc tgcatcatcg atttcgaaac
     1981 gaccggaacg agctgcgacc gcgacaccat caccgagatc ggagcggtga aggtacacgc
     2041 cggcgccgag gtcgcaaggt ttcagaccct cgtgaacccg ggtcgcctga ttccacccca
     2101 ggtgacggta ctcaccggga tcgcccaagc gatggtgacc accgcaccga ggatcgaggc
     2161 ggtgttgccc acactgctcg agttcatcgc cgactgcgtg atggcgggca tggcgcgcag
     2221 cggcagcg
//
LOCUS       DAOOMA010000488         2223 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_61129_length_2223_cov_3.183579, whole genome shotgun sequence.
ACCESSION   DAOOMA010000488 DAOOMA010000000
VERSION     DAOOMA010000488.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 2223)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2223)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2223
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61129_length_2223_cov_3.183579"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>2223
                     /locus_tag="PLP95_11590"
     CDS             <1..>2223
                     /locus_tag="PLP95_11590"
                     /inference="COORDINATES: protein motif:HMM:NF012602.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HPB46488.1"
                     /translation="LINHPLDCPVCDKGGECPLQDTTMAYGPGESRFVEEKRHYRKPI
                     AISETVNLDRERCILCDRCTRFADEVVGDPLIHFMDRGSATQVNTFPGEPFTSYFSGN
                     VVQLCPVGALTATPYRFKARPWDLSEATSTCTGCSVGCRITAQSSRDQLVRFNGVDAD
                     AVNWGWLCDKGRYSYEAINDEGRLTAPLVRDADALVPARWNDALATAARLISDGLGRS
                     GPSGLAVLGGARLSNESAYAWSKLVKGVIGSDNVDAQLGDGLPVDAVLGLPKATIDDA
                     CRPGSTVILLGPDPKEALGALFIRLKHAIVEDGAKLIEVTPQRTGLSRYAASTLVYRP
                     GEAAAVIAALLDGANGDVSGVPADQLNAAKALIDAANGPVTVLLGRANVAESGATIVD
                     AAALLNDRLDGVRFLSTLRRANVHGALDMGLAPGLLPGRVRRADGAAWFSQAWPTVPD
                     SDGLDAIGILQAAADGKIDTLVLLGADPLSDVPDRRLAERALTGARAVIALDTFLNES
                     SRRADVVLAATAAHESAGTHTNLEGRVSLVAHKVTAVATTRTDWSIAAELADRLGHDL
                     GLESAEEIWAEIEELSGIHNGITLELLAQNSVEGVVAPLPAPEPIATGDAEETEAEET
                     VTDGADTEGADGADGEADDIVDEGPELIAAPEPLEFRTPEPTAVPPLDSYSLRIAAGR
                     TMYDAGTTLAHSRSSANLAPVAALRLHPHDFDRVGVASGERVRVSANNRTLVVEAVAD
                     "
BASE COUNT          330 a          753 c          782 g          358 t
ORIGIN      
        1 tgctcatcaa ccacccgctc gactgcccgg tctgcgacaa gggcggcgag tgcccgcttc
       61 aggacaccac gatggcctac ggtccgggcg aaagccggtt cgtggaggaa aagcgccact
      121 accgcaagcc gatcgccatc tccgagacgg tgaacctcga ccgtgagcgt tgcatcctgt
      181 gcgatcgctg cacccggttc gccgacgagg tggttggcga tccgctcatc cacttcatgg
      241 accgtggctc ggcgacccag gtcaacacct tccccggcga gccgtttacc tcgtatttca
      301 gcggcaacgt cgtgcaactg tgcccggtcg gtgcgttgac ggcgaccccg taccgcttca
      361 aggcccgccc gtgggatttg agcgaggcga cctccacctg caccggctgt tcggtgggat
      421 gtcgcatcac ggcgcagtcg agtcgcgacc aactcgtgcg tttcaacggc gtggatgccg
      481 atgcggtgaa ctggggttgg ttgtgcgaca agggccgcta ctcctatgag gcgatcaacg
      541 acgagggccg cttgacggca ccgttggttc gcgatgccga tgcgctggtc ccggcccgct
      601 ggaacgacgc cctcgccacc gcggcgcggc tgatcagcga cggcctcggc cgttcgggcc
      661 cgagcggcct ggcggtgctc ggcggcgccc ggttgtcgaa cgagtcggcc tatgcgtggt
      721 cgaagctcgt caagggtgtg atcgggtcgg acaacgtgga cgcccaactc ggtgacgggt
      781 tgccggtcga cgcggtcctg ggcctgccga aggccacgat cgacgacgcc tgtcgcccgg
      841 gttccacggt catcctgttg ggtcccgacc cgaaggaggc gttgggagcc ctgttcatcc
      901 gcctgaagca cgcgatcgtc gaggacggcg ccaagctgat cgaggtcacc ccgcagcgca
      961 ccgggttgtc gcgttacgcg gcctcgacgc tggtgtatcg cccgggcgag gcggctgcgg
     1021 tgatcgcggc gttgctcgac ggagcgaacg gcgacgtgtc gggggtgccc gccgatcaac
     1081 tcaacgccgc gaaggcgctg atcgatgcgg cgaacggccc ggtcacggtg ttgctgggtc
     1141 gagcgaacgt ggcggagtcg ggtgcgacga tcgtcgacgc tgcggcgctg ctgaacgatc
     1201 gcctcgacgg ggtgcggttc ttgtcgacgc tgcggcgcgc caatgttcac ggagcgctcg
     1261 acatgggcct ggcccccggt ttgttgccgg gccgggttcg ccgtgccgac ggcgccgcct
     1321 ggttctctca ggcgtggccg acggttcccg attccgacgg gctcgacgct atcggcatct
     1381 tgcaggccgc cgctgacggc aagatcgaca ccctcgtgtt gttgggcgcc gacccgctga
     1441 gcgacgttcc cgatcgacgg ttggccgagc gggcgctgac cggcgcccgg gcggtgatcg
     1501 ccctcgacac gttcctcaac gagagttcgc gccgggccga cgtcgtgttg gcggcgaccg
     1561 ccgcacacga gtccgccggc acccacacca acctggaggg ccgggtgagc ctggtcgccc
     1621 acaaggtgac cgcggtggca acgacgcgca ccgactggtc gatcgccgcc gagttggccg
     1681 atcgcctggg ccacgacctg ggcctcgagt cggccgagga gatctgggcc gagatcgaag
     1741 agctgtcggg cattcacaac ggcatcacct tggagttgtt ggcgcagaac tcggtcgagg
     1801 gcgtggtcgc cccgttgccg gctccggagc cgatcgccac cggggacgcc gaagaaaccg
     1861 aggccgaaga aaccgtcact gacggtgccg ataccgaagg cgccgacggt gccgacggtg
     1921 aggccgatga catcgtcgac gagggccccg agctgatcgc cgcccccgaa ccgctcgagt
     1981 tccggacccc ggaacccacg gcggtgcccc cgctcgattc ctactcgttg cgcatcgctg
     2041 cgggccgcac gatgtacgac gccggcacga ccctcgccca ctcgcgtagc tcggccaacc
     2101 tcgctccggt cgccgcactg cggttgcacc cccacgactt cgaccgggtc ggggtcgctt
     2161 cgggtgagcg ggtgcgggtg agcgccaaca accgcacgct cgtggtggag gccgtcgcgg
     2221 acg
//
LOCUS       DAOOMA010000489         2222 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_61178_length_2222_cov_2.717582, whole genome shotgun sequence.
ACCESSION   DAOOMA010000489 DAOOMA010000000
VERSION     DAOOMA010000489.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 2222)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2222)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2222
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61178_length_2222_cov_2.717582"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>2222
                     /locus_tag="PLP95_11595"
     CDS             <1..>2222
                     /locus_tag="PLP95_11595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glutamate synthase central domain-containing
                     protein"
                     /protein_id="HPB46489.1"
                     /translation="ASVVFTDGTVVGAVLDRNGLRPSRYWVTEDGLVVMASEVGTLDI
                     DPASVVEKGRLQPGKMFLIDTDEGRIIGDDEIKSRLAAAKPYQQWLDDNLVHLADLPE
                     VAHVTELESDTRRDQRKFGYTHEELKIILDPMARTGVEPLGSMGTDTPLAVLSNSSRS
                     LFDYFAQLFAQVTNPPLDAIREELVTSVGRVVGPERNLLDPQPESARLITLPRPIIDN
                     DELLKIEHIAELHIGQRTKRLSCLYPVAEGGEGLRRALDQLCTEASKAIEQGYTILLL
                     SDWRSNDELAPIPALLFTSAVHHHLIRDRTRTKVGIVVETGEAREVHHFALLLGYGAA
                     AVNPYLAFDSIEHRIANGLMEGITYEKAVANYIKAVSKGVLKVMSKMGISTVASYTGA
                     QIFEAIGLGQELVDTYFTGTVSRLGGIDLDVIAAEVAARHASAYPKVPEERSHRDLDY
                     GGEYQWRREGEYHLFNPKTVYKLQHSTRTGQYKVFKEYTGMVNDQSRNLATLRGLFEL
                     DVDESRSIPIDEVESVEEIVKRFSTGAMSYGSISQEAHETLAVAMNRLGAKSNTGEGG
                     EDPERLHDPVRRSAIKQVASGRFGVTSEYLTNADDIQIKMAQGAKPGEGGQLPAHKVY
                     PWVAKTRHSTPGVGLISPPPHHDIYSIEDLAQLIHDLKNSNPAARIHVKLVAEVGVGT
                     VAAGVAKAKSDVVLISGHDGGTGASPLTSLKHAGGPWELGLAETQQTLLLNGLRDRIV
                     "
BASE COUNT          429 a          769 c          674 g          350 t
ORIGIN      
        1 ggcctcggtg gtgttcaccg atggcaccgt cgtgggcgcc gtgctcgacc gcaacggcct
       61 gcgtccgagc cgctactggg tgaccgagga cggactcgtc gtgatggcct ccgaggtcgg
      121 aaccctcgac atcgacccgg catcggtcgt cgaaaagggg cgcctccagc ccggcaagat
      181 gttcctgatc gacaccgacg aagggcgcat catcggagac gacgagatca agtcacgtct
      241 cgctgccgcc aagccctacc agcagtggct cgacgacaac ctcgtccacc tcgccgatct
      301 gcccgaggtc gctcacgtga ccgaactcga gtccgacacc cgtcgcgacc agcgcaagtt
      361 cggctacacc catgaagagc tcaagatcat cctcgacccg atggcgcgaa ccggcgtcga
      421 accgctcggc tcgatgggga ccgacacgcc cctcgcggtg ttgtcgaata gcagccgttc
      481 gctgttcgac tacttcgctc agctcttcgc ccaggtcacc aacccgcccc tcgacgcgat
      541 ccgtgaggaa ctcgtcacca gcgtcgggcg cgtcgtgggt ccggaacgca acctgctcga
      601 tccccagccc gagtcggcgc gtctgatcac gttgccccgg ccgatcatcg acaacgacga
      661 gctgctgaag atcgaacaca tcgcggagct gcacatcggt cagcgaacca agcggttgag
      721 ctgcctgtat ccggtcgccg aaggtggcga agggctgcgt cgggcgctcg atcagctctg
      781 cacggaggcc tccaaggcga tcgaacaggg ctacacgatc ctgttgctgt ccgactggcg
      841 ctccaacgac gaactggccc cgatccccgc actgttgttc acctcggccg tgcaccacca
      901 cctgatccgc gatcgcaccc gaaccaaggt cggcatcgtg gtcgagaccg gcgaggcccg
      961 cgaggttcac cacttcgccc tgttgctggg ctacggcgcc gcggccgtca acccgtacct
     1021 cgccttcgac tccatcgaac accgcatcgc gaacggtctg atggagggga tcacctacga
     1081 aaaggccgtc gccaactaca tcaaagcggt gtccaagggg gtgctgaagg tcatgtccaa
     1141 gatgggcatc tcgaccgtcg cgtcctacac cggggcccag atcttcgaag ccatcggtct
     1201 cggccaggaa ctcgtcgaca cgtacttcac gggcacggtg agtcgcctcg gcggcatcga
     1261 cctcgacgtc atcgccgcgg aggtcgccgc ccgccacgcc tcggcctacc cgaaggtgcc
     1321 cgaggagcgc agccaccgcg acctcgacta cggcggcgaa tatcagtggc gtcgtgaggg
     1381 tgaatatcac ctgttcaacc ccaagacggt ctacaagctg cagcactcga cccgaacggg
     1441 ccaatacaag gtgttcaagg aatacaccgg catggtcaac gaccagtcgc ggaacctcgc
     1501 caccctccgc ggcctgttcg aactcgacgt cgacgagtcc cgttccattc ccatcgacga
     1561 ggtcgaatcg gtcgaggaga tcgtcaaacg cttttcgacc ggtgccatgt cgtacgggtc
     1621 gatcagccag gaagcgcacg agaccctggc ggtcgccatg aaccggctcg gcgcgaagtc
     1681 gaacaccggc gaaggcggcg aggaccccga acgattgcac gatccggtgc ggcgctccgc
     1741 catcaagcag gtggcgtccg ggcgcttcgg ggtgacctcg gagtatctga ccaacgccga
     1801 tgacatccag atcaagatgg ctcagggggc caagcccggt gaaggcggtc agcttccggc
     1861 ccacaaggtg tatccgtggg tcgccaagac ccgtcactcg accccgggtg tcggactcat
     1921 cagcccgccg cctcaccacg acatctactc gatcgaagac ctcgcgcagc tcatccacga
     1981 cctcaagaac tcgaatccgg ccgcacgtat ccacgtaaag ctggtcgccg aggtgggggt
     2041 cggcaccgtc gccgcaggtg tggcgaaggc caagtccgac gtcgtgctca tcagtggtca
     2101 cgacggcggc acgggcgcct caccgctcac ctcgctcaag catgccggcg ggccgtggga
     2161 actcggattg gccgaaacgc agcagaccct gctgttgaac gggctgcgcg accggatcgt
     2221 gg
//
LOCUS       DAOOMA010000490         2216 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_61396_length_2216_cov_2.920870, whole genome shotgun sequence.
ACCESSION   DAOOMA010000490 DAOOMA010000000
VERSION     DAOOMA010000490.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 2216)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2216)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2216
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61396_length_2216_cov_2.920870"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1296)
                     /gene="argS"
                     /locus_tag="PLP95_11600"
     CDS             complement(<1..1296)
                     /gene="argS"
                     /locus_tag="PLP95_11600"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR00456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HPB46490.1"
                     /translation="MIRHQLTEALRSHLSAVGIEVDGPIHIEEPQRREHGDWSSNVAM
                     ANAKRNGRVPRELAQELVDRINADLPPNVARAEIAGPGFVNFHLEPTWLHDVLRAVVA
                     AGVQSYARSDTGGGRKVMVEFVSANPTGPLHAGHARGATYGDALARLLDRVGYDVTRE
                     FYINDRGSQMLKMGASMLARATGQAVPEDGYAGAYIAQWATQLTPETVASNDVEAATE
                     VGYRCALADQREVLAELDINFDVWFSERSLVESGAIERTLSDLRDRGVVYDADGAVWL
                     RSTDFGDDKDRVLVKSDGSFTYLLPDIAYHRDKFARGFDLLINVWGADHHGYVPRMRA
                     AIVALGHDAAQFEVQITQLVKLLSNGAEVKISKRTGNIIELRPRRRRHPTGVSAAIPR
                     HPPDGRSRCHRQCVDGQSRVLHPDGSCPAVFDRGEGRRAG"
     gene            complement(1465..1539)
                     /locus_tag="PLP95_11605"
     tRNA            complement(1465..1539)
                     /locus_tag="PLP95_11605"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1504..1506),aa:Arg,seq:ccg)
     gene            complement(1666..>2216)
                     /locus_tag="PLP95_11610"
     CDS             complement(1666..>2216)
                     /locus_tag="PLP95_11610"
                     /inference="COORDINATES: protein motif:HMM:NF036901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB46491.1"
                     /translation="AEGSTYLEQWWSMRPAEAAEMNAALWFGEWGGSPEQDRMADYIT
                     EVLRASDSAMAGWAWWSWDPGGWSPIAEDLTTETANGTALKWVQPRAIAGTPTAFGWD
                     RDAGRFTLEWTERVEAVGTTDIAVPAGLFAEGIEVVVDHDVVESPEWDQQRSVLSLEV
                     DRSLADHTVCIAAAGSGACPAG"
BASE COUNT          371 a          759 c          727 g          359 t
ORIGIN      
        1 ccccgctcgg cggccttcgc cgcgatcgaa cacagccggg catgagccat ctggatgtag
       61 tacacgggat tgtccatcga cgcactggcg atgacaccga gatcgaccgt ctggggggtg
      121 tcgagggatt gcagcagata caccagtcgg gtgacgtcgt cgacgaggtc ggagttcgat
      181 gatgttgccc gtccgcttcg agatcttcac ctcggcacca ttgctgagca gcttcaccaa
      241 ctgggtgatc tgcacctcga actgggcagc gtcatggccc agagcgacga tcgcggcgcg
      301 catgcgcggg acatagccgt gatgatcggc gccccagacg ttgatgagca ggtcgaatcc
      361 gcgggcgaat ttgtcccggt gatatgcgat gtcgggcaac aggtacgtga acgagccgtc
      421 ggacttcacc aacacccggt ccttgtcgtc gccgaagtcc gtcgagcgca gccacaccgc
      481 accatcggcg tcatagacca cgccgcggtc gcgcaggtcg ctcagcgtgc gctcgatcgc
      541 ccccgactcc accaacgacc gctccgagaa ccagacatcg aagttgatgt cgagctccgc
      601 gagcacttcg cgctgatcgg cgagcgcgca ccgatagccg acctcggtgg ccgcctcgac
      661 gtcgttcgac gcgacggtct cgggggtcag ctgggttgcc cactgcgcga tgtaggcgcc
      721 ggcgtaaccg tcctcgggca ccgcctggcc cgttgcccgc gccagcatcg aggcgcccat
      781 cttgagcatc tgcgagccgc gatcgttgat gtagaactcg cgggtgacgt cgtagccgac
      841 ccggtccaac aggcgtgcca acgcgtcgcc gtaggtggcg ccgcgggcgt gaccggcatg
      901 tagcggcccc gtggggttcg ccgacacgaa ctcgaccatg accttgcgac cgccaccggt
      961 gtcggagcgg gcatacgact gcaccccggc ggcgaccacg gcgcgcagca cgtcgtgcaa
     1021 ccaggtcggt tcaaggtgga agttcacgaa tccagggccg gcgatctcgg cgcgtgcgac
     1081 gtttggcggc aggtcggcgt tgatgcgatc gaccagttcc tgggcgagct cgcgtgggac
     1141 acggccgttg cgcttggcgt tcgccatcgc gacgttcgat gaccagtcac cgtgctcgcg
     1201 tcgctgcggt tcctcgatgt ggatcgggcc gtcgacttcg atgccgaccg cggagaggtg
     1261 agaccgaagc gcttcggtga gctggtgacg aatcacgatt tctccatcgc aggtgaatgc
     1321 tggcaacgcc gcccaaccct gcggcgacgg caggtaggcg gtgagggtga gcgggaaggg
     1381 tgagcctact ccccgccgac gaggcgtcga agtgagttca ccgctcggca accgctcggc
     1441 aaccgcccgc ccggcgttgc gatgtggtgc cctcggcagg attcgaacct gcgcacacgc
     1501 ctccggaggg cgatgctcta tcccctgagc tacgagggcg gggatcgaca gcataggcgc
     1561 gctcgcgacg tcgctggcac ctcaaaagcg ttctgggcgc gctagcgggt cacatccgac
     1621 ccgctagcgc gcccagaact cgatcgcgac ggttcaggcg acggttcagc cggccgggca
     1681 cgcgcccgaa ccggccgccg cgatgcacac cgtgtggtcg gccaacgacc gatccacctc
     1741 gaggctcaac accgatcgct gctggtccca ctccggagac tcgacgacgt cgtgatcgac
     1801 cacgacctcg atcccctcgg cgaacagccc tgcgggaacc gcgatgtcgg tggttcccac
     1861 cgcctcgaca cgttcggtcc actccagggt gaaccggccg gcgtcgcggt cccatccgaa
     1921 tgcggtcggg gtgcccgcga tcgcccgggg ctgcacccac ttgagggctg taccgttcgc
     1981 cgtttcggtg gtgagatcct cagcgatcgg cgaccaaccc cccgggtccc agctccacca
     2041 cgcccaaccg gccatggcgg aatcggaggc acgcaacacc tcggtgatgt agtcggccat
     2101 acggtcctgt tcgggcgacc cgccccattc gccgaaccac agcgcggcat tcatctccgc
     2161 cgcctcggcg gggcgcatcg accaccactg ttcgagatag gtcgacccct cggcgt
//
LOCUS       DAOOMA010000491         2213 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_61523_length_2213_cov_4.006024, whole genome shotgun sequence.
ACCESSION   DAOOMA010000491 DAOOMA010000000
VERSION     DAOOMA010000491.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 2213)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2213)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2213
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61523_length_2213_cov_4.006024"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..209)
                     /locus_tag="PLP95_11615"
     CDS             complement(<1..209)
                     /locus_tag="PLP95_11615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB46492.1"
                     /translation="MAQSTDEQLRSLVSGRTVLSEFVASVAAAGPNTFVQWLDGEELV
                     GWTFDEVADKVARVAQGLVDLGVQRG"
     gene            416..1609
                     /locus_tag="PLP95_11620"
     CDS             416..1609
                     /locus_tag="PLP95_11620"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015439668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003985 - acetyl-CoA C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HPB46493.1"
                     /translation="MTTAVLIDAVRTAGGKRNGALSGWHPADLAAETLKAITERNNLD
                     PALIDDVIMGCVSQVGGQALNVGRNAVLAAGFPESVPATTIDRQCGSSQQAAHFAAQG
                     VMSGAYDIAIAAGVEVMTLVPMGASVGNGKSGFPFGEAMGLRYAERGGLVPQGISAEM
                     IAEKWGLTRDELDAFSLESQRRAAQAIAEGRFDNEIVPVRKKVLDKESREVRVLDEMH
                     TIDEGVRESSLEGLGRLKPSFKEDGLVHAGNSSQLCDGASAALIMSEDKANELGLKPR
                     ARFHTFALAGVDPVYMLTGPIPSTQKALERSGLSIDDIDLFEVNEAFAPVVLAWAKEL
                     GADLAKTNVNGGAIALGHPLGASGTKLMATLINELERTGGRYGLQTMCEGGGLANATI
                     IERLD"
     gene            1649..1975
                     /locus_tag="PLP95_11625"
     CDS             1649..1975
                     /locus_tag="PLP95_11625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB46494.1"
                     /translation="MRVSLIAWIVGAAIAFAVAAFVFDTGWLNAIVAIAAAAAILKVG
                     LMIIGSLATPIPEPPDEGELRKVKITYRCEICGAQVRMTFAASQTPEPPRHCMEEMEQ
                     IETPDI"
BASE COUNT          422 a          744 c          687 g          360 t
ORIGIN      
        1 ccccgctgca caccgagatc aaccaggccc tgagcgacgc gtgcgacctt gtcggcaacc
       61 tcatcgaagg tccacccgac gagttcctcg ccgtccaacc actgcacgaa ggtgttcggc
      121 ccagcggcgg cgaccgatgc gacaaattcg gagagcaccg tgcggccgct caccagcgac
      181 cgcagttgtt cgtcggttga ttgagccatc gagcatctcc cttgcgaagg tcccacactc
      241 cggtgagcgt gcgtacgagc cacatcagcg taacggcgat gtgactttgg ttgcgaacgc
      301 cccacagaaa cgacgcgagc cacgcggccc tgattgaccg gcgaggcacc tgcggagcac
      361 actcttgacc gaccggtcta gttttgttgg gaccacgaca aggggacgaa tcatcatgac
      421 caccgcagta ctcatcgacg cagtacgcac cgccggcggc aagcgaaacg gcgcattgtc
      481 aggctggcac cccgccgacc tcgccgccga gacgctcaaa gccatcaccg agcgcaacaa
      541 cctcgatccg gcgctcatcg acgacgtgat catggggtgc gtcagccagg ttgggggcca
      601 ggcgctcaac gtcggacgca atgccgtcct ggccgccggc tttcccgaga gcgtgcccgc
      661 caccaccatc gaccgccagt gcggctccag ccaacaagcc gcccacttcg cggcccaggg
      721 ggtcatgtcg ggcgcctatg acatcgcaat cgcggccggc gtcgaggtca tgaccctggt
      781 cccgatgggg gcatcggtcg gtaacggcaa gtccggattc ccgttcggcg aagcgatggg
      841 tctgcgctac gccgagcgcg gcggcttggt gccccaaggc atctccgccg agatgatcgc
      901 cgagaagtgg gggctcaccc gcgacgaact cgacgcgttc tccctcgaat ctcagcgccg
      961 cgccgcgcag gccatcgccg agggtcggtt cgacaacgag atcgtgcccg tgcgcaagaa
     1021 ggtcctcgac aaggaatccc gcgaagttcg ggtcctcgac gagatgcaca cgatcgatga
     1081 aggcgtgcgc gaaagctccc tcgagggact tggacgcctg aagccctcgt tcaaggagga
     1141 cggcctggtc cacgccggca actccagcca gctctgcgac ggcgcttccg ccgcgctcat
     1201 catgagcgag gacaaggcga acgagctcgg cctcaagccc cgggcccgct tccacacgtt
     1261 cgcactcgcc ggtgtcgacc cggtgtacat gctcaccggt ccgatcccat cgacccagaa
     1321 ggcgctcgaa cgttccggcc tgtcgatcga cgatatcgat ctgttcgagg tgaacgaggc
     1381 cttcgccccc gtcgtgctcg catgggcgaa ggaactcggc gccgatctgg cgaagacgaa
     1441 cgtgaacggt ggcgcgatcg ctctcggcca cccgctcgga gcctcgggca ccaagctgat
     1501 ggcgacgttg atcaacgaac tcgagcgcac cggcggccgc tacggcctgc agacgatgtg
     1561 tgagggcggc ggcctcgcga acgccaccat catcgagcgt ctcgactgaa gaaccgattc
     1621 acgtcgagcg acgtgggaga ctgacgtcat gcgagtgtcc ctcatcgcct ggatcgtggg
     1681 tgcagccatc gcgtttgcgg tggctgcatt cgttttcgac accgggtggc tcaacgcgat
     1741 cgtcgccatc gccgccgcgg ccgccatttt gaaggtcggg ttgatgatca tcggcagttt
     1801 ggcgacgccg atccctgaac ctcccgatga gggcgagctt cgcaaggtga agatcaccta
     1861 ccgctgcgag atctgcggcg cgcaggtgcg catgaccttc gccgcgtcac agactcccga
     1921 gcctccacgc cactgcatgg aggagatgga acagatcgaa acccccgaca tctgaccggc
     1981 agacggcgtc gccgcgaggc tgagcgggtc gagagttgtc cccagcctgg ggataactct
     2041 ggggacttgc gccgcttcgc ccgtgtcgat tattgcgatc gagtgtcgtt catgaccact
     2101 cgcggaccgc tcggtgacgc ggacgcaacg tcaggtgaaa cattcaacgt caactcgcga
     2161 gttacccaca tatccacaac aaactgtgga taaggccctt gcggcgacgg ttc
//
LOCUS       DAOOMA010000492         2199 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_62117_length_2199_cov_3.074627, whole genome shotgun sequence.
ACCESSION   DAOOMA010000492 DAOOMA010000000
VERSION     DAOOMA010000492.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 2199)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2199)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2199
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62117_length_2199_cov_3.074627"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            140..1261
                     /locus_tag="PLP95_11630"
     CDS             140..1261
                     /locus_tag="PLP95_11630"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020379092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HPB46495.1"
                     /translation="MYIGMVCPYSLSVPGGVQGQVFGLARALRSRGHVVQIIAPADGP
                     TTEGGVMVVGQSVMNAANGSVAPISPNAPTQIRTMRMLWDENFDVLHLHEPLVPGPSV
                     TALLLKPAPVVGTFHAAGDQPAYSSFSSLARWVGGRIDAKVAVSEEARRLAGQSIPEP
                     WTVLFNGIEIDQFAGAEPWDRPDEAGDEGKPAVFFLGRHEERKGLGVLLEALSEMDDD
                     VVVWIAGTGPETLALKAKYRDDKRLRWLGRISNEERNRRLAAAAAFVAPSLGGESFGV
                     ILLEAMAAGAPVVCSDITGYVGVAGPLDGEPAAARITRTGDSRSLAEGLAEVLRDRSL
                     AEVLREHGRRRANRFSMDRLAEAYTVLYEAVIAEAAMRA"
     gene            1375..1929
                     /locus_tag="PLP95_11635"
     CDS             1375..1929
                     /locus_tag="PLP95_11635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020379049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HPB46496.1"
                     /translation="MKWVVIVLIILIVLVAVLLIANYNGLVGLRNKIESAYSQIDVQL
                     NRRYSLIPNLVETVKGYAAHESGTLEKVVQARNLAMNTNGIADKAEAENAITGALKSV
                     FALSEAYPELKANTNFQQLQAELSSTEDRIAYARQYYNDNVRAYNTKIEAFPSNLIAN
                     AFKFTQREYFQAQGDARGDVSVSF"
     gene            1937..>2199
                     /locus_tag="PLP95_11640"
     CDS             1937..>2199
                     /locus_tag="PLP95_11640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB46497.1"
                     /translation="MTPNSTKSTTGTAGTAGTAGTDRGQHRSLLERSDLNSKAIAMIA
                     RPHAIVAGVASFVVSAVVIGFLISWPVGVIVGLLVGGVAALATL"
BASE COUNT          374 a          703 c          749 g          373 t
ORIGIN      
        1 tgcagcggtt catgcaggtt ctcgccggcg agctcgaaac gctgatccgc cgcgcacccg
       61 agcaatggca ccttctgcag ccgaactggc cgagcgattt cgaactcctc ggcgccccgc
      121 ggccgacgtt gagctgacca tgtacatcgg aatggtctgt ccctacagcc tgagcgtccc
      181 cggcggcgtt cagggtcagg tgttcggttt ggcgcgggcg ctgcgaagcc gcggtcacgt
      241 ggtgcagatc atcgcgccgg ccgacgggcc caccaccgag ggcggggtca tggtcgttgg
      301 gcagtcggtc atgaacgccg ccaacggctc ggttgccccc atctcgccga acgccccgac
      361 tcagatccgc acgatgcgca tgctgtggga tgagaacttc gacgtgttgc acctgcacga
      421 accgctcgta cccggaccgt ccgtgaccgc actgttgctg aagccggccc ccgtggtcgg
      481 caccttccac gccgccggag accaacccgc gtactcctcg ttttccagcc tcgcccggtg
      541 ggtcggggga cgaatcgacg ccaaggtggc ggtgagcgag gaggcccgac gcctcgccgg
      601 ccaatcgatt ccggaaccgt ggaccgtgtt gttcaacggg atcgagatcg atcagttcgc
      661 cggggccgaa ccgtgggacc gccccgacga ggcgggcgac gaaggaaagc ctgcggtgtt
      721 cttcctcggc cgtcacgagg aacgcaaggg cctcggggtg ctgttggagg cgttgtcgga
      781 gatggacgac gacgtcgtgg tgtggatcgc cgggacgggg cccgaaacgc ttgctctcaa
      841 ggcgaagtac cgcgacgaca agcggctgcg gtggctcggg cgaatcagca acgaggaacg
      901 caaccgtcgc ctcgctgcgg ccgcggcctt cgtggccccg tcgctcggtg gcgagagctt
      961 cggggtgatt ctgctggagg cgatggccgc cggcgctccg gtggtgtgca gcgatatcac
     1021 cggatacgtg ggcgttgccg gcccgctcga cggcgagcct gctgcggcgc gcatcacccg
     1081 gaccggcgac agtcgatcgc tcgccgaggg gctcgcggag gtactccgcg accggtcgtt
     1141 ggccgaggtg ttgcgcgagc acgggcgccg gcgcgccaac cggttttcga tggaccgcct
     1201 cgccgaggcc tacaccgtgt tgtacgaggc ggtcatcgcc gaagcggcca tgcgggcgtg
     1261 atccgaggcc gccagtgggc cttgttcggt cgctgacgac ggccccccct ggggtgggtc
     1321 gggttgccta tcatggggcg gcgcggcaac gagtcgccgg tcgaggagcc gtccatgaaa
     1381 tgggttgtca tcgttctgat catcctcatc gtgttggtcg cggtgttgct gatcgccaat
     1441 tacaacgggc tggtcgggct gcgaaacaag atcgagtcgg cgtactccca gatcgacgtc
     1501 caactgaatc gccggtactc gttgatcccg aacctggtcg agacggtcaa ggggtatgcg
     1561 gcgcacgagt cggggacgct cgaaaaggtg gtgcaggcca gaaacctggc gatgaacacc
     1621 aacgggatcg cggacaaggc cgaggccgaa aacgccatca ccggagcgct caagagcgtg
     1681 ttcgcgttga gtgaggcgta ccccgagttg aaggccaaca cgaactttca gcagctgcag
     1741 gccgagttga gttcgaccga ggaccgcatc gcgtatgccc ggcagtacta caacgacaac
     1801 gtgcgggcct acaacaccaa gatcgaagcc tttccctcga acctgatcgc caacgccttc
     1861 aagttcaccc aacgcgaata cttccaagcc caaggcgacg ctcgcggcga cgtctccgtc
     1921 tcgttctgaa acgcttatga caccgaactc caccaaatcg accaccggaa ccgctgggac
     1981 cgccgggacc gccggaacgg atcgcggcca gcaccgatca ttgctcgaac gcagcgacct
     2041 caattccaag gccatcgcga tgatcgcccg accgcacgcg atcgtggccg gtgtggcgtc
     2101 gttcgtggtg tcggcggtgg tgatcgggtt cttgatctcg tggcccgtcg gggtgatcgt
     2161 cgggctgttg gtcggcggtg tcgccgcctt ggcgaccct
//
LOCUS       DAOOMA010000493         2199 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_62130_length_2199_cov_2.387593, whole genome shotgun sequence.
ACCESSION   DAOOMA010000493 DAOOMA010000000
VERSION     DAOOMA010000493.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 2199)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2199)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2199
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62130_length_2199_cov_2.387593"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            280..1353
                     /locus_tag="PLP95_11645"
     CDS             280..1353
                     /locus_tag="PLP95_11645"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPB46498.1"
                     /translation="MSHPTAAQPNADAVVSLRHVTHRFGEVTALNSLSFDVPSGGITV
                     LLGPNGAGKTTAIRAITGAISPSGTIRTFGIDPRVDGDSVRPHCGVVSAKPALYDRLS
                     GRDNLEYAAELYGVARGDIDSRVSESAERFGITHALDHLVGGYSTGMKTRLALARAVL
                     HRPELLLFDEPTSGLDPESSAAVLDLIREMTSLGITVVMCTHLLSEAEGLADHIVILE
                     TGSCVCEGPTAQLTRRFWPHDTVVFDAVAPDQLDRIATWPGVVGYDRADLARVQLDDI
                     GRIPAIVSSLVADGVQLTRVEPVVPTLEQLYFAVRSGRGQDRFGRPAAPTAAPTAAPA
                     HAANPARAHANALAGSASQGGRR"
     gene            1350..>2199
                     /locus_tag="PLP95_11650"
     CDS             1350..>2199
                     /locus_tag="PLP95_11650"
                     /inference="COORDINATES: protein motif:HMM:NF024091.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HPB46499.1"
                     /translation="MTATNMTTTPITRPRFSGHRMWVVARTELKQLAGAKDYWIPMAL
                     LGSLFFIVLPAILLLTVSSWGDTEVVTQVSQSLRVLPKSAQAQISRIDEGGQTLYALS
                     VFLFAPVAVIVPITISTAVGAATIVGERERGTGEFLAHSPASAREIYLGKLIASFVPG
                     YLTTLVGFGCYSVLVNTIAGPAVGGWFFPTAQWWVMVLWIIPPFLALTLSLVLRLSAT
                     VRSTAAAQQASGLISLPLIGIAYGQSSGAVFGQTNAGIWVGAIAWAFAVVSLTRGVRS
                     VTRARLL"
BASE COUNT          344 a          838 c          631 g          386 t
ORIGIN      
        1 cgcccgaaac gactcacgaa cccctcaaca cgggccccat aaacagcgac ccctcatcag
       61 gggcccttgg cgcgcacacg aacggcgcgc acacgaacgg cgcgcgcgca aaccggctga
      121 ctcccgtggg ctgtagggat gcagggttcg tgccgataga tcgatgtgcc gccttttcgc
      181 cgttcgccat ctcgccagcc gctctcccag cagtctgtag ctcacccgcc gtcagcccaa
      241 cagtcatcag tccagccgcc gtcggcccag cagtcgccag tgtcccatcc gacagcggcc
      301 caaccgaatg ccgacgctgt cgtgtcgttg cggcacgtga cccatcgatt cggcgaagtc
      361 actgccctca actcgctgtc cttcgatgtc ccctcgggcg gcatcaccgt cctgctcggg
      421 cccaacgggg ccggcaagac caccgccatc cgagcgatca ccggcgcgat ctctccatct
      481 ggaacgatcc gaacgttcgg tatcgaccct cgcgtcgacg gcgacagtgt acgtccgcac
      541 tgcggagtcg tcagcgcgaa gccggcgttg tatgaccgtc taagcggacg agacaacctt
      601 gagtacgcgg cagagctcta cggcgtcgcc cggggtgaca tcgactcgcg cgtctctgag
      661 tccgcagagc gcttcggcat cacccatgcc ctcgaccacc tcgtcggcgg ctattccacc
      721 gggatgaaga ctcgtctcgc gctggcccgc gccgtgctac atcgccccga attgttgctc
      781 ttcgacgagc caacctcggg cctcgacccg gaatcctcgg ccgcggtcct cgacctcatc
      841 cgggagatga cctccctcgg catcaccgtc gtgatgtgca cccacctcct ctccgaagcc
      901 gaagggctcg ccgatcacat cgtcatcctc gaaaccggta gctgcgtatg tgaagggccc
      961 accgctcaac tcacccgacg cttctggccc catgacaccg tcgtgttcga cgccgtcgca
     1021 cccgaccaac tcgaccgaat cgccacctgg cctggcgtcg tcggatacga ccgtgccgac
     1081 ctcgcccggg tacagctcga cgacatcggc cgcatcccgg ccatcgtctc ctcgctcgtc
     1141 gccgacgggg ttcagctcac gcgggtcgag ccggtggttc ccactctcga acagctctac
     1201 ttcgccgtcc gcagcggccg cgggcaggac cgtttcggcc gccccgcagc ccccaccgca
     1261 gcccccaccg cagcacccgc tcacgccgcc aaccccgccc gtgcccatgc caatgccctg
     1321 gccggctctg cctcccaagg aggtcgacga tgaccgcgac caacatgacg actacaccga
     1381 tcactcggcc gagattcagc ggccaccgaa tgtgggtcgt cgcccgcact gaactcaaac
     1441 agctcgccgg cgccaaggac tactggatcc cgatggcgct gctgggctca ttgttcttca
     1501 tcgtgctgcc cgcgatcctg ttactcaccg tgtcgtcgtg gggtgacacc gaggtcgtca
     1561 ctcaggtctc ccagtcgctg cgggtgctgc cgaagtcggc gcaggcgcag atctcgcgca
     1621 tcgacgaggg cggccaaacc ctctacgcgc tgtcggtgtt cctgttcgcc cccgtggcgg
     1681 tcatcgtgcc gatcacgatc tccaccgcgg tcggcgccgc caccatcgtc ggcgaacgag
     1741 agcgaggcac gggcgaattc ctcgcccact cccccgcctc ggcccgcgaa atctacctcg
     1801 gcaagctcat cgccagcttt gttccgggct acctcaccac cctggtggga ttcgggtgtt
     1861 actcggtgct ggtcaacacg atcgccggtc ccgccgtcgg aggctggttc ttccccacgg
     1921 cccaatggtg ggtcatggtg ctgtggatca tccccccgtt cctcgcgctg accttgtcgc
     1981 tggtgcttcg gctctcggcg acggttcgat cgaccgctgc agcccaacag gcctccggcc
     2041 tcatcagcct gccgttgatc ggtatcgcct acgggcagtc atccggggcg gtgttcgggc
     2101 agaccaacgc cggcatctgg gtcggggcca tcgcctgggc gttcgcggtt gtgagcctca
     2161 cccgcggcgt acgttcggtc acgcgggcac gcctcctcg
//
LOCUS       DAOOMA010000494         2192 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_62401_length_2192_cov_4.681797, whole genome shotgun sequence.
ACCESSION   DAOOMA010000494 DAOOMA010000000
VERSION     DAOOMA010000494.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 2192)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2192)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2192
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62401_length_2192_cov_4.681797"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1113)
                     /locus_tag="PLP95_11655"
     CDS             complement(<1..1113)
                     /locus_tag="PLP95_11655"
                     /inference="COORDINATES: protein motif:HMM:NF012296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HPB46500.1"
                     /translation="MTQPNPSPRQPQDAPAGARFARDITDVDLSLAASFAQAVPHGAF
                     DALREAGGVAWHDELPVDDAMQTDFVRFVDSPGFWTVTSHELVNEVLRNSDRFSSELG
                     TTMMLSLDEESLPTLRAMLINMDAPHHTRIRRILQPSFTPRSVEKLHESVRVNAREIM
                     VELDGQRECELVTSVSAELPLRVLADLLGMPRSDRHLIFEWSNALIEAEGIQQSGESA
                     SGVEAMAAMVEYGQAMAAQRREHPTDDMVSTIANAQVDGDRLDDWEFAMFWVLLVVAG
                     NETTRNAISGSVAALQEHRRWSQMTAQGEVPMVALEELLRYVSPVAHFRRTATADTTL
                     GDQHIRAGDKVVVWFGAANRDPNVFSNRHGLELDRAE"
     gene            complement(1221..1856)
                     /locus_tag="PLP95_11660"
     CDS             complement(1221..1856)
                     /locus_tag="PLP95_11660"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HPB46501.1"
                     /translation="MTPRLQCRGLTVGYGSLTVVRNVDLSLSDGTVLALLGPNGAGKT
                     TMLSAMAGLLAHQSGEVILDGTVLPSGKAQAANRAGLVLVPDNRCLFTTLSVGDNLRA
                     AAPKDRDAIEEVAEMFPALRRRWKVTAGSLSGGEQQMLAIGRALVQRPRALLIDELSM
                     GLAPLIVTQLLDTVRRAADEMNTVVVLVEQHVRLALDVADEASCSFTARPF"
     gene            complement(1853..>2192)
                     /locus_tag="PLP95_11665"
     CDS             complement(1853..>2192)
                     /locus_tag="PLP95_11665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB46502.1"
                     /translation="RSLTAGPRVLLLDEPAAGLDSTESEWLAQRLRDIRTAGVSILLV
                     DHDMALVLGLCDRIEVLDQGRIIASGPPEEIRRSRHVAEAYLGAPATSATATSATVTG
                     KPASRPVEAP"
BASE COUNT          350 a          710 c          761 g          371 t
ORIGIN      
        1 ctcggcgcga tcgagttcga ggccgtgccg attcgaaaac acgttggggt cgcggttcgc
       61 cgcaccgaac cacacgacca ccttgtctcc ggcgcggatg tgttggtcgc caagagtggt
      121 gtcggcggtc gcggtgcgcc gaaagtgggc caccggcgac acgtagcgca acagctcctc
      181 aagcgcgacc atcggaacct caccttgggc tgtcatctgg gaccagcgtc ggtgttcctg
      241 gagtgccgcg accgacccgg agatggcgtt gcgggtggtc tcgttgccgg cgacgaccaa
      301 cagcacccag aacattgcga actcccagtc gtcgagccgg tcgccgtcga cctgcgcgtt
      361 ggcgatcgtc gagaccatgt cgtcggtggg atgttcgcgc cgctgcgccg ccatcgcctg
      421 cccgtactcg accatcgccg ccatcgcttc gaccccgctg gccgactcgc ccgactgttg
      481 tatgccctcg gcctcgatga gcgcgttcga ccactcgaag atcagatggc gatccgaacg
      541 cggcatgccc aacagatcgg cgagcacccg caacggcagc tccgccgaga cggaggtgac
      601 gagttcgcac tcgcgttggc catcgagttc caccatgatc tcgcgggcgt tgacccgcac
      661 cgattcgtgc agcttctcaa ccgaacgcgg tgtgaagctc ggttggagaa tccggcgaat
      721 acgcgtgtga tgcggcgcgt ccatgttgat gagcatcgcc cgcaaagtcg gcaggctctc
      781 ctcgtcgagg gacagcatca tcgtcgtgcc gagttcggag gagaaccggt ccgaattacg
      841 aagcacctcg ttgacgagtt cgtggctcgt gacggtccag aaccctgggc tgtcgacgaa
      901 gcggacgaag tcggtctgca tcgcgtcatc gacgggcaac tcgtcatgcc aggcgacacc
      961 tccggcctca cgcaacgcgt cgaaggcccc atggggcacc gcctgtgcga acgaggcggc
     1021 gaggctgagg tcgacgtcgg tgatgtcgcg ggcgaaacgg gcgccggcgg gcgcgtcctg
     1081 tggctgtcga ggtgatggat tgggctgcgt cattcggggt tcctttcccg tcggtaccgg
     1141 actggtggtt cagcggttct cgccgaggta ggcggcctcg acccgtccga tgtcggcggc
     1201 aagctcgtgg gccggggcgc tcagaacggc ctcgccgtga acgagcagga ggcctcatcg
     1261 gcgacgtcga gcgcgagacg aacgtgttgt tcgaccagca ccacgacggt gttcatctca
     1321 tcggcggcgc gccgcactgt gtcgagcagt tgggtcacga tgagcggagc gagacccatc
     1381 gacaactcat cgatcaacaa cgcccggggc cgttgcacga gggcccgacc gatggcgagc
     1441 atctgctgtt cgccgccgga cagcgatccg gcggtcacct tccaacggcg cctgagggcg
     1501 gggaacatct cggcgacctc ctcgatggcg tcgcggtcct tgggcgcagc cgcgcgcagg
     1561 ttgtctccga cgctgagcgt ggtgaacagg cagcggttgt cggggaccag cacgaggccg
     1621 gcgcggttcg cggcctgcgc cttgccggag ggcagcacgg ttccatcgag gatcacctcg
     1681 ccgctctggt gcgccaacaa tccggccatc gcggacagca tcgtggtctt gccggcgccg
     1741 ttggggccga gcagtgccaa cacggtgcca tcggacagcg acaggtcgac gttgcggaca
     1801 acggtgaggc ttccgtaacc gacggtgagg cctcggcatt gcagtcgcgg ggtcatggcg
     1861 cctccaccgg gcggcttgcg ggcttgccgg tcacggtcgc gctggtcgct gtcgcgctgg
     1921 tggctggcgc tcccaggtac gcctcggcga cgtgtcgact tcttcggatc tcctcgggtg
     1981 gaccgctggc gatgatgcgg ccctgatcga gcacttcgat gcggtcacac agccccaaca
     2041 ccagggccat gtcgtggtcg accaacaaga tgctgacccc ggcggtgcgg atgtcgcgaa
     2101 ggcgctgcgc aagccattcg ctctcggtcg agtcgagccc ggccgccggt tcgtcgagga
     2161 gcagcacccg ggggccggcg gtgagcgagc gg
//
LOCUS       DAOOMA010000495         2190 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_62487_length_2190_cov_4.310070, whole genome shotgun sequence.
ACCESSION   DAOOMA010000495 DAOOMA010000000
VERSION     DAOOMA010000495.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 2190)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2190)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2190
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62487_length_2190_cov_4.310070"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(129..1841)
                     /locus_tag="PLP95_11670"
     CDS             complement(129..1841)
                     /locus_tag="PLP95_11670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HPB46503.1"
                     /translation="MNESAGANRSRWNEAFEASPLRDADFASMSGVEVDAVYGPDPVR
                     GGNSEVPGPHDETTDEATGEGQYPGQYPYTRGPYASMYRSKLWTMRMFAGFGTAVDTN
                     LRFRDILANGGDGLSTAFDLPTLMGYDSDDPMSLGEVGRCGVAVDTQADMEDLFRGID
                     LESTTTSMTINSPAAPIFAMFIAAAEAAGMRRERIGGTLQNDILKEYQAQKEFIFPPR
                     PSLRLVRDTMTFCAAEMPRWHALSVSGYHIREAGSTAAQELAFTLANGFAYVELGLAA
                     GLAIDEFAPRLSFFFNAHSDFFEEIAKYRAARRIWARWLRDRYGATNPKSMQLRFHTQ
                     TAGVSLTAQQPEVNIVRTAIEALAGVLGGTQSLHTNSMDETLALPTEKAARIALRTQQ
                     VIAYETRVTNVADPLGGSWYVEALTDDMEALAEEIFSKIAEIGDGSMYEGCVIGVEDG
                     WFQGEIANSAYELQRKVDDGRHIIVGVNAFTEGNDEPAPETLYIDGSVEDQQLKRLDA
                     VRSERDTSRVEAALDRVRRDAADPEINLMPALLEASTAMVTLGEMMSAMADVFGRWDE
                     TPRI"
     gene            1840..2094
                     /locus_tag="PLP95_11675"
     CDS             1840..2094
                     /locus_tag="PLP95_11675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB46504.1"
                     /translation="MGATVSAAVSAAVRIPGGQTVAVTSPDQLPDTIVCVDCGGVCHR
                     LNRGFEGGDQAGDVVSYRCEDCLDRWDLVAGDPEDLDEFG"
BASE COUNT          346 a          728 c          731 g          385 t
ORIGIN      
        1 gacctcgtag ccggcgtcgc gcaacagccg cgccaccacc ttgaccccgc ggtcgtggcc
       61 gtcgaggccg agtttggcca ccagcacccg cccgagcggc acggccgatt cgttgaagaa
      121 gtccatcgtc agatcctcgg ggtctcgtcc caccggccga acacatcggc catcgccgac
      181 atcatctcac cgagggtcac catcgcggtc gacgcctcca acaacgccgg catcagattg
      241 atttcgggat ccgcggcgtc gcggcgcacc cgatcgaggg cggcctcgac acggctggtg
      301 tcgcgttcgg agcgcaccgc gtcgagtcgt ttgagctgtt gatcctcaac cgagccgtcg
      361 atgtagaggg tctcgggagc cggctcgtcg ttgccctcgg tgaacgcgtt cacgcccacg
      421 atgatgtgac ggccgtcgtc gacctttcgc tggagttcgt acgccgagtt cgcgatctcg
      481 ccttggaacc agccgtcctc aacgccgatc acgcagcctt cgtacatcga accgtcgccg
      541 atctcggcga tcttggagaa gatctcctcg gcgagcgcct ccatgtcgtc ggtgagcgcc
      601 tccacgtacc acgagccgcc gagcgggtcg gccacgttgg tcacgcgggt ctcataggcg
      661 atgacctgct gggttctcag cgcgattcgg gcggcctttt cggtgggcag cgcgagggtt
      721 tcgtccatcg agttcgtgtg caggctctgg gtgcctccga gcacgccggc gagtgcctcg
      781 atcgcggtgc ggacgatgtt cacctcgggc tgttgggcgg tgagcgacac cccggcggtc
      841 tgggtgtgga accgcaactg catcgacttc gggttggtgg ctccgtagcg gtcgcgcaac
      901 caacgggccc agatgcgacg agcggcgcgg tacttcgcga tctcttcgaa gaagtcggag
      961 tgtgcgttga agaagaagct cagcctcggt gcgaactcgt cgatcgccag tccggcggcc
     1021 aggcccaatt cgacgtaggc gaaaccgttg gcgagcgtga atgccagttc ctgggccgcg
     1081 gtcgatccgg cttcgcggat gtgatacccg gaaaccgaca gcgcgtgcca gcggggcatc
     1141 tcggccgcgc agaacgtcat ggtgtcgcgg accagacgca gcgacggccg cggcggaaag
     1201 atgaattcct tctgggcctg atactccttg aggatgtcgt tctgaagcgt cccgccgatg
     1261 cgctcacgcc gcatcccggc ggcctcggcc gcggcgatga acatcgcgaa gatcggcgcc
     1321 gccggagagt tgatggtcat cgaggtcgtg gtcgactcga ggtcgatgcc ccgaaacagg
     1381 tcctccatgt ccgcctgggt gtcgaccgcc accccgcatc ggccgacctc gccgaggctc
     1441 atcgggtcat cggagtcgta gcccatcaac gtcggcagat cgaacgccgt cgacagcccg
     1501 tcgccgccgt tggcgaggat gtcgcggaac cgaaggttgg tgtcgacggc ggtgccgaac
     1561 ccggcgaaca tgcgcatcgt ccagagcttc gagcgataca tcgaggcgta cgggccgcgg
     1621 gtgtagggat actgccccgg gtactggccc tcgccggtgg cctcgtcggt ggtctcgtcg
     1681 tgtgggccgg gcacctcgga gttcccaccg cgaacggggt ccgggccata gacggcgtcg
     1741 acctccacac ccgacatcga cgcgaagtcg gcgtcacgca gcggcgacgc ctcgaatgcc
     1801 tcgttccaac gggaccgatt cgcaccggcg gattcgttca tgggtgccac ggtatcggca
     1861 gcggtatccg ccgcggtgcg cattcccgga gggcaaactg ttgcggtgac ctcgccagac
     1921 cagcttccgg acacgatcgt gtgcgtcgac tgcggcggcg tgtgtcaccg cctcaaccgg
     1981 ggtttcgagg gcggcgacca ggccggcgac gtcgtgtcgt accgttgcga ggactgtctg
     2041 gaccgttggg acctcgtcgc cggcgaccca gaagatctcg acgagttcgg ctgatcggcc
     2101 gagccgctcc ccctttccag gaaggccctc catgaccgaa accgacgcac tccccgcctc
     2161 cgacggtcgt ctcgacgatc ttgccggtgg
//
LOCUS       DAOOMA010000496         2189 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_62536_length_2189_cov_3.722587, whole genome shotgun sequence.
ACCESSION   DAOOMA010000496 DAOOMA010000000
VERSION     DAOOMA010000496.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 2189)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2189)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2189
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62536_length_2189_cov_3.722587"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..953)
                     /locus_tag="PLP95_11680"
     CDS             complement(<1..953)
                     /locus_tag="PLP95_11680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020378885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018580 - nitronate monooxygenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HPB46505.1"
                     /translation="MKNELCERLGIEFPIFAFTHCRDVVAAVSNAGGLGVLGAVGYTP
                     EQLETELAWIDEHVGDKPYGVDIVIPMKYEGMDADMDPDALQDMLRDMIPAEHRAFVK
                     KLLADHDVPELPDGEYPELIGWTEATAGPQVEIALSHPKVKLIANALGTPPADIVKEI
                     QDSGRLVAALCGSVKHAIGHKEAGIDIVIAQGGEGGGHTGDVGSIVLWPQVADAIAPT
                     PMLAAGGIGSGRQMAAAMAMGAAGVWTGSLWLTVEEADLPPAQMQQLLDATSRDTVRS
                     RSFTGKPCRMLRNEWTDAWENPENPQPLGMPQQFMITGEAVA"
     gene            1128..>2189
                     /locus_tag="PLP95_11685"
     CDS             1128..>2189
                     /locus_tag="PLP95_11685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB46506.1"
                     /translation="MVEISTSCRRRRARRAVSAVAVGTFALVVLARPFNSASTEESAD
                     NVTDTGGGGVTAQRFVPPIDTSDLILEDVSGAVILRDGATRQASAVVRNRGGGTVDDV
                     VMRFKLGDDVVFDRATSSAGCELNVGQVICPVGSLGSGESRTVTVGLSVPSPGAAVGA
                     RDSYFLAPVSQLADLNAGEIRERSWALGYGITGSPLLSCWALDARSPNMASPSSGCGT
                     SGSPPLDPNSERVLERFSDAMRSSAVGSYEWLTEITPATSGTYKVCAAGLGDAAYVAV
                     GPTTSALVNDDVVYELDGYDSEARQRTFDLDQGVSYRVLARLSTHRYDPTTGAADIGW
                     DYLGITLADRPCTQASAGVF"
BASE COUNT          369 a          733 c          705 g          382 t
ORIGIN      
        1 gcgaccgcct caccggtgat catgaactgc tgcggcatgc cgagtggctg cgggttctcg
       61 gggttctccc acgcgtcggt ccactcgttg cgcagcatcc ggcacggctt gccggtgaag
      121 ctgcggctgc gcacggtgtc acggctggtc gcgtcgagga gctgttgcat ctgcgccggg
      181 gggaggtcgg cttcctcgac ggtcagccac aacgatccgg tccacacacc agctgcgccc
      241 atcgccatgg cagctgccat ctggcgaccg gaaccgatgc cgcccgccgc cagcatcggg
      301 gtcggggcga tcgcgtccgc aacctgtggc cacaacacga tcgaaccgac gtcgccggtg
      361 tgcccgccgc cctcgccgcc ctgggcgatg acgatgtcga tgccggcttc cttgtggccg
      421 atcgcgtgct tgaccgaacc gcacagcgcg gcgacgagac ggccgctgtc ttggatctcc
      481 ttgacgatgt cggcgggagg ggttccgagc gcgttggcga tgagtttcac cttgggatgc
      541 gacagcgcga tctccacctg cgggccggcg gtcgcctcgg tccaaccgat cagttcggga
      601 tactcgccgt cggggagctc tgggacgtcg tgatcggcga gaagcttctt gacgaacgcc
      661 cggtgctcgg cggggatcat gtcgcgcaac atgtcctgaa gcgcgtcggg atccatgtcg
      721 gcgtccatcc cctcgtactt catcgggatg acgatgtcga cgccgtaggg cttgtcaccg
      781 acgtgttcgt cgatccacgc cagctcggtt tcgagctgtt caggtgtgta gcccacggcg
      841 ccgagcacgc cgagaccccc ggcgttggag acggcagcga caacgtcgcg acagtgagtg
      901 aaggcaaaga tcgggaactc gatcccgagg cgttcgcaga gctcgttctt catatggaaa
      961 atgaatacca cagaactaga acgcgttccc ataattgttg aacgcacgtt caagaggcgc
     1021 cgtttggtcc gacttgatag gcaatatcgc ccaacgaact ggtcaatcga cctcgacaac
     1081 gcttgactgc aacctgcgac acgacgaagc ccccgaggag tgcgccggtg gtcgaaatct
     1141 caacctcatg tcggcgccgc cgagcccgac gcgccgtatc cgccgtcgcc gtcggcacgt
     1201 tcgcgttggt cgtgctcgcc cgccccttca actcggcctc aaccgaggag tccgcggaca
     1261 acgtcaccga caccggcggt gggggcgtca ccgcccaacg gttcgtccca ccgatcgaca
     1321 cgtcagatct catcctggaa gacgtcagcg gtgctgtgat cctgcgcgac ggggcgacac
     1381 gacaagcctc cgctgtggtg cggaaccgcg gcggaggcac cgtcgacgat gtcgtgatgc
     1441 gtttcaagct cggcgacgat gtggtgttcg atcgtgcaac gtccagcgct gggtgcgaac
     1501 tcaacgtcgg gcaggtgatc tgtccggtcg gatcgctcgg cagcggcgaa tcacgcaccg
     1561 tcaccgtcgg actctcggtg ccctcccccg gcgcagccgt gggagcacgc gacagctact
     1621 tcctcgctcc ggtcagccaa ctcgccgacc tgaacgcggg cgagatccgc gagcgctctt
     1681 gggcactcgg gtacggaatc accggtagcc cgctcctgag ttgctgggcg ctcgatgccc
     1741 ggagcccgaa catggcatca ccgagcagcg gctgtggcac cagcggaagt cccccgctcg
     1801 atccgaattc cgagcgggtg ctggagcgct tttccgacgc gatgcgatcc agcgctgtcg
     1861 gctcctatga atggttgacc gaaatcacgc cggcgacctc tggcacctac aaggtgtgcg
     1921 cggccggtct cggcgatgcg gcctacgtcg cagtcggccc gaccaccagc gcgctggtca
     1981 acgacgacgt ggtctacgaa ctcgacggtt acgacagcga ggcacgacag cgaaccttcg
     2041 atttggacca aggcgtgtcc tatcgggtgt tggctcggct ctccacccac cggtacgacc
     2101 ccaccacggg cgccgccgat atcgggtggg attacctcgg aatcacgctc gccgaccggc
     2161 cctgcaccca ggccagcgcc ggggtgttc
//
LOCUS       DAOOMA010000497         2187 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_62622_length_2187_cov_4.569887, whole genome shotgun sequence.
ACCESSION   DAOOMA010000497 DAOOMA010000000
VERSION     DAOOMA010000497.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 2187)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2187)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2187
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62622_length_2187_cov_4.569887"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..457)
                     /locus_tag="PLP95_11690"
     CDS             complement(<1..457)
                     /locus_tag="PLP95_11690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020378865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavin oxidoreductase"
                     /protein_id="HPB46507.1"
                     /translation="MSRRYPQVKTMRTPQQLRDRLDELQLDLPVVDEVHPDGPLADSV
                     SFSDIAAGTITTPNRFVALPMEGWDGTTDGRPTELVRRRWERFAASGCGLVWGEATAV
                     VPEGRANPNQLVIRHESVEEFAALRSVLDPSQVVGLQLTHSGRWSRPEGA"
     gene            complement(454..1449)
                     /locus_tag="PLP95_11695"
     CDS             complement(454..1449)
                     /locus_tag="PLP95_11695"
                     /EC_number="4.1.3.3"
                     /EC_number="4.2.1.41"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020378863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HPB46508.1"
                     /translation="MVSTPPQRLGREVVAMSAILVPLLDDRSIDWAALEAHIARTAAA
                     GLTPAVNMDTGYVQLLSSADRRRVLELARRVTDAPDRQDAFVAGAFVGDRAGDAYDRA
                     ALAKAANEVAAHGGTPVIFPNHGLGGLDDDSWVAAMTELGDELDRFIGFELGEMFVPY
                     GRILGLDAYEGLLGVRSCIGAKHSSLSRTLEWQRLELRDRVRPGFHVFTGNDLAIDMV
                     VYGSDYLLGLSTFAPDLFAIRDRMWRDGDARFWEVNDLLQYLGMFAFRDPVPAYRHDA
                     AMFLELRGWTSSSEVPDGAPRRPDADRDVLADIARRVDALVDELSSEVEAGGATP"
     gene            1531..>2187
                     /locus_tag="PLP95_11700"
     CDS             1531..>2187
                     /locus_tag="PLP95_11700"
                     /inference="COORDINATES: protein motif:HMM:NF013920.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold domain-containing protein"
                     /protein_id="HPB46509.1"
                     /translation="MRPLPHLTPTNTPFWTSGADGVLRVQGCLDCRRLVHPPVPRCPH
                     CGSLNHEPTEVSGRGVVAGHTTNLQQWLPGFAPPYVIANVALDDDPEVHLTTNIVDCD
                     PDEVAIGSVVQVRFEQIDDVWVPLFTLTGETVANPVAEPETPRVRAPLTKDRFEHRSV
                     LSGVGKSRIGRRLLVDPLSLTVDACLAAVADAGLTLDDIDGLATYPGAVGMGMSEGGV
                     S"
BASE COUNT          372 a          750 c          715 g          350 t
ORIGIN      
        1 gcgcaccctc cgggcgcgac cagcgccccg aatgggtgag ttgtaggccg acgacctgac
       61 tcgggtcgag caccgaccgc aacgccgcga actcctcgac cgactcgtgg cggatcacga
      121 gttggttcgg attggcccga ccctcgggca ccaccgcggt cgcttcgccc cacaccaacc
      181 cacatccact cgcggcgaag cgttcccacc ggcggcgcac cagttcggtc gggcgaccgt
      241 cggtggtgcc gtcccacccc tccatcggaa gcgcgacgaa tcgattcggt gtggtgatgg
      301 tgccggccgc gatgtcgctg aacgagacag agtcggcgag ggggccgtcg gggtgaacct
      361 catcgacaac ggggaggtcg agttggagct cgtcgagacg atcgcgtagt tgctgcgggg
      421 tacgcatcgt cttgacctgc ggatagcggc gactcatggt gtcgctccgc ccgcttcgac
      481 ctcggaggac aactcgtcca cgagcgcgtc aacgcggcgc gcgatgtcgg cgagcacgtc
      541 gcgatcggca tccggtcgcc tgggcgcccc gtcggggact tcgctgctcg aggtccaacc
      601 gcgcagttcg aggaacatgg cggcatcatg gcgataggcg gggacggggt cgcggaaggc
      661 gaacatgccg agatactgca acaggtcgtt gacctcccag aaccgtgcgt caccgtcacg
      721 ccacattcga tcacggatgg cgaacaggtc gggggcgaac gtcgacaggc ccaggaggta
      781 atccgagccg tacaccacca tgtcgatcgc caagtcgtta cccgtgaaca catggaaccc
      841 gggccggacc cgatctcgaa gttcgaggcg ttgccactca agcgtgcgcg acagcgagga
      901 gtgcttcgcc ccgatacagg accgcacgcc gagtagtcct tcgtacgcgt cgaggccgag
      961 gatgcggcca tagggcacga acatctcgcc gagctcgaaa ccgatgaacc ggtcgagttc
     1021 gtcgccgagt tcggtcatgg ctgcgaccca gctgtcatcg tcgagaccgc cgagcccgtg
     1081 gttcgggaag atgaccgggg tgccgccgtg tgctgcgacc tcgtttgccg ccttggccag
     1141 cgccgcacgg tcgtacgcgt ccccggcgcg gtcaccaacg aacgcaccgg cgacgaacgc
     1201 atcttggcgg tcgggcgcgt cggtgacccg tctggcgagt tccaacacgc gtcgccgatc
     1261 ggcactcgac agaagctgga catagccggt gtccatgttg acggcgggag tcaggccggc
     1321 cgcggcagtt ctcgcgatgt gggcctcgag cgcggcccag tcgatcgatc ggtcgtcgag
     1381 cagcggcacg aggatggccg acatggcgac gacttctcgt cccagacgtt ggggtggggt
     1441 actcaccatg gttggcagcc tgtcacggaa ccgccgcctt cgtcgcgtca ttcgtggcca
     1501 ctcaagcaag tgaacgctaa ctttcagtac gtgcgaccac tcccccacct cacgccgacg
     1561 aacaccccct tttggacctc cggggccgac ggggtgttac gagtgcaggg ttgcctcgac
     1621 tgccgacgcc tcgtccatcc gcccgtaccg cgctgtccac actgcggcag cctcaaccac
     1681 gaacccaccg aggtgtccgg acgcggagtc gttgccgggc acacgacgaa cctgcagcag
     1741 tggcttcccg gattcgcacc gccctacgtg atcgccaatg tcgccctcga cgatgacccc
     1801 gaggtccacc tcacgaccaa catcgtcgac tgcgaccccg acgaggtcgc catcgggtcg
     1861 gtcgtgcagg ttcgcttcga gcagatcgac gacgtgtggg ttccgctgtt cacactcacc
     1921 ggcgagaccg tcgccaaccc ggtcgccgaa cccgagaccc ccagggttcg cgccccgctc
     1981 accaaagacc gcttcgagca ccgcagtgtg ttgtcgggcg tcggcaagtc gcgcatcggt
     2041 cgacgccttc tggtggatcc gctgtcgctg accgtcgacg cctgcctcgc tgcggtggcc
     2101 gacgccgggc tgaccctcga cgacatcgac gggctcgcca cctaccccgg agcggtcggc
     2161 atggggatga gcgagggcgg tgtctcc
//
LOCUS       DAOOMA010000498         2175 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_63120_length_2175_cov_3.997170, whole genome shotgun sequence.
ACCESSION   DAOOMA010000498 DAOOMA010000000
VERSION     DAOOMA010000498.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 2175)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2175)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2175
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63120_length_2175_cov_3.997170"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1210)
                     /locus_tag="PLP95_11705"
     CDS             complement(<1..1210)
                     /locus_tag="PLP95_11705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012230387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="B12-binding domain-containing radical SAM
                     protein"
                     /protein_id="HPB46510.1"
                     /translation="MTSLWNELEPLLHRVSKPARYIGCEQGAQTPEHGSGVVSWLLIY
                     PDTYEIGLPNQGLQILYEILNERPDAAAERAYAPWVDLEAELRAKGLPLFSVDTHRPA
                     PLFDLLAFNLSAELNYTNVLNCVDLSGVPVRAADRSPDHAFVVAGGHCTYNPEPLADF
                     VDFVVLGDGEEVVGEITETIGDWKADGRGSREEVLRRLASIEGVYVPSMYEVAYEGRH
                     IRSVTPRYPELPEVVPKRTISDLAEWPYPKQQLVPITEVVHDRLNVEVFRGCTRSCRF
                     CQAGMITRPVRERPADQVRTMVADGLKRTGYDEVALTSLSTADMSGIADIVSGIVGDP
                     DNCGQVSVSLPSLRVDAFTVGIAAQIQKARRTGLTFAPEAGTWRMRQVINKMIREEDL
                     YGAVNSAYSQG"
     gene            complement(1305..>2175)
                     /locus_tag="PLP95_11710"
     CDS             complement(1305..>2175)
                     /locus_tag="PLP95_11710"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: protein motif:HMM:NF013279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HPB46511.1"
                     /translation="LSPTMYLVVVAMLVAVWRFARPIKGIRAWFELGPIQIQPAELAK
                     FVVIVLVATYLGSIDGQLKLRHLITSLVMFAIPMGLIMLQPDLGTNLVFVMIAAGMLV
                     CAGFPLRYIVTLVLVVVVGVVAILNSDTLEDYQRDRLTSFASADSDSYNTRQSQTAIS
                     LGGLAGAGYLQGEYTKGGSVPEQQTDFIFTVSGEEFGFLGSSVLLLLLAGIIWRVWRT
                     AQVARDRVGSLMCIGVLCLFMFHVFENVGMSLGIMPVTGIPLPFVSYGGSAMITAFAC
                     VGLVQNVHMRRYH"
BASE COUNT          424 a          703 c          694 g          354 t
ORIGIN      
        1 acccctggga gtaggccgag ttcaccgccc cgtagaggtc ttcctcgcgg atcatcttgt
       61 tgatgacttg gcgcatgcgc cacgtgccgg cttcgggagc gaaggtcaat ccggtccggc
      121 gggccttttg aatctgcgcc gcgatcccga cggtgaacgc gtcgacgcgc agcgacggca
      181 acgacaccga gacctgcccg cagttgtcgg gatcgccgac gatgccgctg acgatatcgg
      241 cgatgccgct catgtcggcg gtcgacagcg aggtcagcgc cacctcgtcg taaccggtgc
      301 gcttcaggcc gtcggcgacc atggtgcgaa cctgatcggc cgggcgttcc cggacggggc
      361 gggtgatcat gcccgcctgg cagaaccggc agcttcgggt gcagccgcgg aagacctcga
      421 cgttgagccg atcgtggacc acctcggtga tcggcacgag ttgctgtttc ggatacggcc
      481 actccgcaag gtcggagatg gtccgcttcg gcaccacctc gggcaactcg gggtaccgcg
      541 gggtgaccga acggatgtgt cgaccctcgt aggcgacctc atacatcgag ggaacgtaga
      601 cgccctcgat cgacgcgaga cggcggagca cctcctcgcg cgagcctcga ccgtcggcct
      661 tccaatcgcc gatggtttcg gtgatctccc cgacgacctc ttcgccgtca ccgagcacca
      721 caaagtcgac gaaatccgcc agcggctcgg ggttgtaggt gcagtggccg ccggcgacga
      781 caaaggcgtg gtccgggctg cgatccgctg cccggacggg aacgcccgag agatcgacgc
      841 agttcaagac gttggtgtag ttgagctcgg ccgacaggtt gaacgccaaa aggtcgaaca
      901 gcggcgctgg tcggtgggtg tcgacgctga acagcggcag accctttgcc cgaagctctg
      961 cctcgagatc gacccacggc gcgtaagccc gctcagcagc cgcgtcgggg cgctcgttga
     1021 ggatctcgta gaggatctgc aaaccttgat tcggtaggcc gatctcgtag gtgtcggggt
     1081 agatgagcag ccatgaaacg acgccggagc catgctccgg tgtctgcgca ccctgttcgc
     1141 agccgatata gcgggccggc ttggaaacgc ggtgcagcag cggttcgagc tcgttccaca
     1201 gcgacgtcat ggtgcgacca tgctactcag caaggtgtcg ctgcgctgaa ccacgcgtaa
     1261 cgccggtgtt gccgccgctc caccagcgtc cgacaacggt gcgctcagtg gtatcgacgc
     1321 atgtgcacgt tctgcacgag accgacgcag gcgaacgcgg tgatcatcgc cgaaccgccg
     1381 taggaaacga acggaagcgg aatgccggtc accggcatga tgcccagact catcccgacg
     1441 ttttcgaaca catggaacat gaacaggcac aacacgccga tacacatcag cgaacccacg
     1501 cggtctcggg cgacctgagc ggtgcgccac accctccaga tgatcccggc gagcaacagc
     1561 agcagcacgc tcgatccgag aaacccgaac tcctcgccgg acacggtgaa gatgaagtcg
     1621 gtctgctgtt cggggaccga accgcccttg gtgtattcac cttgcaggta cccggcgccg
     1681 gcgaggccgc cgaggctgat cgcggtctgg ctctggcggg tgttgtagct gtcgctgtcc
     1741 gcgctcgcga acgacgtcag gcgatcgcgt tgatagtcct ccagggtgtc ggaattgagg
     1801 atcgccacca ccccgacgac caccaccagc accagggtca cgatgtagcg cagcggaaac
     1861 ccggcgcaca ccagcatgcc tgcggcgatc atcacgaaca ccaggttggt tcccaggtcg
     1921 ggctgcaaca tgatgaggcc catcggaatc gcgaacatca ccagcgaggt gatcaggtgc
     1981 ctcagtttca gctggccgtc gatgctgccg aggtacgtag ccaccaacac gatgacgacg
     2041 aacttcgcaa gctcggcggg ctgaatctga atcgggccga gctcgaacca ggctcgaata
     2101 cctttgatcg gccgggcgaa cctccacacc gccaccaaca tcgccaccac gaccaggtac
     2161 atcgtcggcg acagc
//
LOCUS       DAOOMA010000499         2175 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_63126_length_2175_cov_3.366509, whole genome shotgun sequence.
ACCESSION   DAOOMA010000499 DAOOMA010000000
VERSION     DAOOMA010000499.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 2175)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2175)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2175
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63126_length_2175_cov_3.366509"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..241
                     /locus_tag="PLP95_11715"
     CDS             <1..241
                     /locus_tag="PLP95_11715"
                     /inference="COORDINATES: protein motif:HMM:NF015953.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HPB46512.1"
                     /translation="AALIIGIVNALIGPVLKLLSLPLTILTFGLFALVVNAVLLLLAA
                     EVAPGFEIDTFWSGLFAAILYSIVTSIGSSLLLND"
     gene            complement(370..1158)
                     /locus_tag="PLP95_11720"
     CDS             complement(370..1158)
                     /locus_tag="PLP95_11720"
                     /inference="COORDINATES: protein motif:HMM:TIGR03083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maleylpyruvate isomerase family
                     mycothiol-dependent enzyme"
                     /protein_id="HPB46513.1"
                     /translation="MDSSTMLRRFEDEARRFAELVAATPADSAVPTCPGWTLTRLVVH
                     QGSILHWARALIDANGEYLDRRSLDIGLPGVDASPQVWADWLLESATLVIEALERIPD
                     DTPTWTWGDGTTAAWWTRRLLHETAVHRIDAELAAGNTDWTIDAEVAIDAIDEHLANV
                     EASGSFCDAVTHLRGDGSIHLHTTDLDGEWLIDLNPHGFTIGHGPRAATTATRQTVAA
                     RGTARDLLAAVTNRASSTNGLPDGLQIFGEPGMLVWWFTNSALT"
     gene            complement(1259..2029)
                     /locus_tag="PLP95_11725"
     CDS             complement(1259..2029)
                     /locus_tag="PLP95_11725"
                     /inference="COORDINATES: protein motif:HMM:NF016908.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FkbM family methyltransferase"
                     /protein_id="HPB46514.1"
                     /translation="MRYLKHFGLPRTVKYLVLDRPGATGFLELTWEGYPAPFRLRRGG
                     TDLRTFVHVIAGEGYQLPWDLKSEPRWIIDAGANCGLSAVYFAKTYPTATVIAIEPDE
                     ENFRLLVHNTASYDNIVCVKAALWPRSGSINLVDPGVGAWGYRAADSTDPGAEDAAEP
                     GGPNTDASLPSTEIAAVTVEELIADHGIDEIGIFKVDIEGGEHAVFGSADGWIDRVET
                     IAIELHDRFQPGCTEVFEAATAEFTHRVERSEDLFVGR"
BASE COUNT          355 a          679 c          715 g          426 t
ORIGIN      
        1 cgctgcgctc atcatcggca tcgtgaacgc gctcatcggt cctgttctca agctgctgtc
       61 gctgccgctg acgatcctga ccttcgggct gttcgctctc gtggtgaacg ccgtgttgtt
      121 gttgctcgcc gctgaggtgg cgccaggctt cgaaatcgac accttctgga gcggactgtt
      181 cgccgcgatt ctctacagca tcgtcacctc gatcgggtcg tcgctgctcc tcaacgactg
      241 atcgtcaacg actgatcgtc aacgactgct cctcaacgac tgatcgtcaa cgactgatcg
      301 tcaacgactg atcgtcaacg actgatcctc aacggcgggc cccaaagcgc tcgggctcac
      361 ggccgggcat cacgtgagcg cgctgttggt gaaccaccac acgagcatcc cgggttcacc
      421 gaatatctgg aggccgtcgg gaagcccgtt ggtgctcgag gctcggttgg tcaccgcggc
      481 gaggaggtct cgcgccgttc ctctcgcggc caccgtttgc ctcgtggccg tcgtcgcagc
      541 gcgtgggccg tggccgatcg tgaacccgtg ggggttgaga tcgatcagcc attcaccgtc
      601 gaggtcggtg gtgtgcaggt ggatcgagcc gtcgccacgt aggtgggtaa ccgcgtcgca
      661 gaacgacccc gatgcctcga cgttggcgag gtgttcgtcg atcgcgtcga tcgcgacctc
      721 ggcatcgatc gtccagtcgg tgttgcctgc cgcaagctcg gcgtcgatgc ggtgaaccgc
      781 cgtttcgtga agcaggcggc gagtccacca agcagcggtg gtgccgtcgc cccaggtcca
      841 ggtgggggtg tcgtcgggga ttcgctcgag cgcctcgatg accagggtcg ccgactccag
      901 cagccaatca gcccagacct gcgggctcgc gtcgactccc ggcaggccga tgtcgagcga
      961 tctgcgatcg aggtattcgc cgttggcgtc gatcagcgct cgggcccagt ggagaatcga
     1021 accctggtgg acgaccaagc gggtcaaggt ccaccccgga caggtcggta cggcgctatc
     1081 ggcgggggtg gcggcgacca attcggcgaa acgccgcgcc tcgtcctcga agcgtctgag
     1141 catcgtcgag gaatccatgg cctcagtctg tcacgctcgt cgctcgtcgc ccgtcacgac
     1201 agggttcgat cagcgttcgc ctgaatctgc gttcgaccgg atccgtggcg ggtgggattc
     1261 agcggccgac gaaaaggtct tcgctgcgct ccacccggtg ggtgaattcg gcggtggccg
     1321 cctcgaagac ctcggtgcag cccggctgga aacggtcgtg gagttcgatg gcgatcgttt
     1381 cgacccggtc gatccatccg tcggcggaac cgaacacggc gtgttcaccg ccctcgatgt
     1441 cgaccttgaa gattccgatc tcgtcgatgc cgtggtcggc gatcaactcc tcgacggtga
     1501 ctgctgcgat ttcggtgctc ggcagcgatg catcggtgtt cggtccgcca ggttcagcgg
     1561 cgtcctcggc cccgggatcg gtggaatcgg ccgctcggta accccatgcc cccaccccgg
     1621 gatcgacgag gttgatcgat cccgaccgcg gccacaacgc cgccttcacg cagacgatgt
     1681 tgtcgtagga agcggtgttg tgaacgagaa ggcggaagtt ctcctcgtcg ggttcgatgg
     1741 cgatgaccgt ggcggtcggg taggtcttcg cgaagtacac ggctgataat ccgcagtttg
     1801 ctccggcgtc gatgatccaa cgcggctcgg atttgaggtc ccagggcagt tgatagcctt
     1861 cgccggcgat gacgtggacg aaggttcgca ggtcggtccc gccgcggcgg agccgaaacg
     1921 gcgccgggta cccctcccag gtcagctcga gaaatcccgt tgcgccggga cggtcgagga
     1981 cgaggtattt caccgtcctt gggagaccaa agtgcttgag gtatctcatg attccacgtg
     2041 aaatcgtctg cactctggta cccgaactcg gccctgacat ggccggcgag gctacgaacc
     2101 gcacgtgggg gcgtcaagca ccgttctccg ataaccagtg gtcgtgatca cgaccactgg
     2161 ttatcggaga acggg
//
LOCUS       DAOOMA010000500         2171 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_63277_length_2171_cov_3.468809, whole genome shotgun sequence.
ACCESSION   DAOOMA010000500 DAOOMA010000000
VERSION     DAOOMA010000500.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 2171)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2171)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2171
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63277_length_2171_cov_3.468809"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..301)
                     /locus_tag="PLP95_11730"
     CDS             complement(<1..301)
                     /locus_tag="PLP95_11730"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HPB46515.1"
                     /translation="MGDHIWTPGDPRTCNEVLDRRLDTDPDGDYLDVCGTRFSAAEVE
                     SVANRIANSYAGLGVSPGDPVATLIENSPEAMLVWWGAVRGNVVAVPINTAYKGDY"
     gene            complement(304..1143)
                     /locus_tag="PLP95_11735"
     CDS             complement(304..1143)
                     /locus_tag="PLP95_11735"
                     /inference="COORDINATES: protein motif:HMM:NF016777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HPB46516.1"
                     /translation="MTRVIDCLVNVDFADRYMANYMTRVKEDYFKADESFFSSPELGE
                     LIDLMDEKGVEKAILICQVNDVDTRSLRFVEARPDRFALGVGGFDLLKPMKNLANLEA
                     FVANNPVAYASVGPSFWGDGMYPPTDAVYYPLYVKCCELDLALSMNMGMPGPPLPAEP
                     QNPIHLDRVLYRFPELRLVMMHGADPWWEVAIRIMLKYRNVRMMTSAWAPKYLPEVLL
                     HFMRTRGKERVMFASDYPVLKHERLLAEADALDLPDDVRDNWLYGNANSFFFRENSFG
                     EEA"
     gene            1316..>2171
                     /locus_tag="PLP95_11740"
     CDS             1316..>2171
                     /locus_tag="PLP95_11740"
                     /inference="COORDINATES: protein motif:HMM:NF014790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HPB46517.1"
                     /translation="MTNSSTTATTDEPHHELREELRAWLAENWDPGLSVEVWWRIVGD
                     AGWTAPHLPPEQGGRGLFRTAVNTVRTMFAEAGAPRPPGGLGLLMAAPTILAQGTPEQ
                     IERHVPPILRGEANWCQLFSEPGSGSDLAGLTTRADRDGDRWIINGQKVWSSMAHESD
                     YGMLLARTNWDVPKHAGISWFAFKMDQPGVTVRPLREMTGDAVFNEVFFDNAVVDHAD
                     LVGGQDNGWTVTQTTLFFERTGLSSGAHAGWPVPGPKGGFWGRNAADAGRDELPTTDI
                     MVRYPELVE"
BASE COUNT          387 a          670 c          725 g          389 t
ORIGIN      
        1 ggtagtcgcc tttgtaggcg gtgttgatcg gcacggcgac gacgttgccg cgcaccgcgc
       61 cccaccacac gagcatcgcc tcgggcgagt tctcgatcag cgtggccaca gggtcgccgg
      121 gcgaaacgcc cagtcccgca taggaattcg cgatgcggtt cgccaccgat tcgacctcgg
      181 cggcgctgaa tctcgtcccg cagacatcga ggtagtcgcc gtcgggatcg gtgtcgaggc
      241 gtcggtccag cacctcgttg caggtgcgcg gatcgcccgg ggtccagatg tgatcgccca
      301 cgctcaggcc tcttcaccga agctgttctc ccgaaagaag aacgagttgg cgttgccata
      361 cagccagttg tcgcgaacgt cgtcgggcaa gtcgagcgcg tctgcctcgg cgagcagtcg
      421 ttcgtgtttg agcaccgggt agtcgctggc aaacatcacc cgttccttgc ctcgggtccg
      481 catgaaatgc aacagcacct caggcagata cttcggcgcc caggcagagg tcatcatgcg
      541 cacgttgcgg tacttcaaca tgatgcggat cgctacctcc caccacggat cggcgccgtg
      601 catcatgacg aggcgcagtt cggggaaccg atagagcact cggtcgaggt ggatcgggtt
      661 ctgcggctcg gcgggcagcg gcgggccggg cattcccatg ttcatcgaca gcgccaagtc
      721 gagttcgcag cacttcacat acagcgggta gtagacggcg tcggtgggcg gatacatgcc
      781 gtcgccccaa aagctcggtc cgaccgacgc gtaggccacc gggttgttgg cgacgaaggc
      841 ctcgaggttc gccaggttct tcatcggctt gagcagatcg aagccgccga cgccgagggc
      901 gaagcgatct ggtcgggcct cgacgaaacg cagcgaacgg gtgtcgacat cgttgacctg
      961 gcagatcagg atcgccttct cgacgccctt ttcatccatc agatcgatga gttcaccgag
     1021 ttcggggctc gagaagaacg actcgtcggc cttgaagtag tcctctttga cccgcgtcat
     1081 gtagttggcc atgtagcggt cggcgaagtc gacgttgacg agacagtcga tcactcgagt
     1141 catgcggtcc ccctgagatc cgaacggtgt tgcacctgcc gcggccgcgg cctgtgccgg
     1201 cagcgttttg gaacatccta cggtgtttgg aaagtcctac tgtacacatt tggaaagtga
     1261 cgcttcacaa tcgtgtacgg tgctcaggcg accgattcga agggggaaca tcagcgtgac
     1321 gaacagttcg acaaccgcga cgaccgacga gcctcatcac gaactgcgcg aggaacttcg
     1381 ggcatggctg gccgagaact gggaccccgg cctgagtgtc gaagtgtggt ggcgcatcgt
     1441 cggcgacgcc ggctggaccg ccccgcatct cccgccggaa cagggtggcc gcgggctgtt
     1501 tcgtaccgct gtcaacacgg tgcggacgat gttcgccgag gccggagccc ctcgcccgcc
     1561 gggagggctc gggctgttga tggccgcgcc gaccatcctc gcccagggca cccccgaaca
     1621 gatcgagcgc cacgtcccgc cgatcctgcg cggcgaggcg aactggtgtc agctgttcag
     1681 cgagccgggg tctgggtcgg acctggcggg gttgacgacg cgggccgacc gcgacggcga
     1741 ccgttggatc atcaacggtc agaaggtctg gagctcgatg gcacacgagt ccgactacgg
     1801 catgttgttg gcccgcacga attgggatgt gcccaagcac gccgggatct cgtggttcgc
     1861 attcaagatg gatcagccgg gggtgaccgt gcggccgctg cgtgagatga ccggcgacgc
     1921 cgtgttcaac gaggtgttct tcgacaacgc ggtggtcgac cacgccgatc tcgtcggtgg
     1981 tcaggacaac ggttggaccg tcacccagac gaccctcttc ttcgaacgca ccggattgtc
     2041 gagcggggcc catgccggat ggccggtgcc ggggcccaag ggtgggttct ggggacgcaa
     2101 cgccgcggac gccggccgtg acgaactccc gaccaccgac atcatggttc gctacccgga
     2161 gttggtggag t
//
LOCUS       DAOOMA010000501         2167 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_63448_length_2167_cov_4.072917, whole genome shotgun sequence.
ACCESSION   DAOOMA010000501 DAOOMA010000000
VERSION     DAOOMA010000501.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 2167)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2167)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2167
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63448_length_2167_cov_4.072917"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..348)
                     /locus_tag="PLP95_11745"
     CDS             complement(<1..348)
                     /locus_tag="PLP95_11745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB46518.1"
                     /translation="MSARRGQDSTAATVTATLIAVVGGVALGRSGLGSGSAGWLAAIG
                     LVVVVGLSALWAMLSRPDRRLGSLSTKAELVAAAAAAAAATAPHPQGPVALRSVAMLR
                     RSAVFSERTVAEVL"
     gene            506..1315
                     /locus_tag="PLP95_11750"
     CDS             506..1315
                     /locus_tag="PLP95_11750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020379087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HPB46519.1"
                     /translation="MPPTSASSSSSSSALEMADVSVVRGDRTVLADVNWLVEPHQRWV
                     LLGANGCGKTTLARIAALYDHASSGTVRVLGETVGRTDVRSLRKQVAFVSAAFVDLLR
                     GDLIAADVVMCAINASLEPWWHTYSDDDRAAARAALASLGIGHLADRRFGSLSSGERQ
                     RVQLARALMTAPDLLVLDEPTAGLDLRGREEFVRDLDAIALDGPAMILVTHHLEEIPE
                     TFDRVMILGSGAVIAQGDIDTCLTDASLSESAGVDVTIQRHGRRWAARAAF"
     gene            complement(1359..1940)
                     /locus_tag="PLP95_11755"
     CDS             complement(1359..1940)
                     /locus_tag="PLP95_11755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB46520.1"
                     /translation="MDINATLRTDLRAEELYPVLIDLATYPEWIGIVGRAVAEVDPPD
                     AVRDAATTGAVRTDAGTTGESSARESSTGVAGDSSAPDDSDEPIEAWSIDLRGQLGPL
                     RRSKRLRMVRTEATINRYVRFDRAELDGRQHSEWVLTADLNERPDGTELTMRLHYGGT
                     MWMPMLDRLLRDEIERSRARLVAYTSSHRASGR"
     gene            complement(1945..>2167)
                     /locus_tag="PLP95_11760"
     CDS             complement(1945..>2167)
                     /locus_tag="PLP95_11760"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HPB46521.1"
                     /translation="GAVVEPGTVLGYVGNTGAPGSATHLHFEIHPAGGAPVNPFPLLR
                     TVWGDRPMRSESAVAAAIAAVAAVPEEER"
BASE COUNT          336 a          777 c          711 g          343 t
ORIGIN      
        1 caacacctcc gcgaccgtgc gttcgctgaa cacagcacta cgtcgcaaca tggcgaccga
       61 ccggagcgct acgggcccct ggggatgcgg agcggtcgcc gccgccgctg ccgccgccgc
      121 tgcgaccagc tccgccttgg tggacaggga gcccagccga cgatcgggac gcgacagcat
      181 ggcccacagc gccgacaacc cgacgacgac gaccagaccg atggccgcca gccaccctgc
      241 cgaccccgac cccaaccccg atcgcccgag cgcgacaccg ccgaccaccg cgatgagggt
      301 ggccgtgacg gtggccgccg tcgagtcctg gccgcgcctg gcgctcatcg ctgtcaacct
      361 atcgcccctc ggccgatgcc ccgccgcgac cggcgcgatc tggccggcgg gtccggcgcg
      421 atctggccgg cgggtccggc gcgatctggc cggcgggtcc ggcgcgatct gacgttacgg
      481 cagcacagcg gccacgatga tgacgatgcc gcccacctca gcctcatcat cgagttcgtc
      541 gtccgccctc gagatggccg acgtttcggt cgtgcgcggc gaccgcaccg tgcttgccga
      601 tgtgaactgg ttggtcgaac cgcatcaacg ctgggtcctg ctcggggcga acggatgcgg
      661 caagaccaca ctcgcccgca tcgcagcgct ctatgaccac gccagttccg gcacggttcg
      721 ggttctgggc gaaaccgtcg gccgcaccga cgtgcgctcg ctgcgcaagc aggtggcgtt
      781 tgtgagcgcg gccttcgtcg atctgctccg tggggacctg atcgccgcgg acgtggtgat
      841 gtgtgcgatc aacgcctcac tcgagccgtg gtggcacacc tacagcgatg acgatcgcgc
      901 cgcggcgcgc gccgcgctcg cctcactcgg gatcggccac ctcgccgatc ggcgtttcgg
      961 gtcgctgtcg agcggggaac gccagcgggt acaactcgcc cgagcgctca tgaccgcgcc
     1021 cgatctgttg gtccttgacg agccgaccgc cggtctcgac cttcgcggcc gggaggagtt
     1081 cgtccgcgac ctcgacgcaa tcgccctcga cggccccgca atgatcctcg tcacccacca
     1141 cctcgaagag atcccggaaa ccttcgaccg cgtgatgatc ctgggctccg gcgccgtgat
     1201 cgcccaaggt gacatcgaca cctgcctcac cgacgcatcg ttgagcgagt cggcgggggt
     1261 cgatgtgacg attcaacggc atggacgacg ctgggcggct cgggcagcgt tttgaccgtg
     1321 gcccatgttc tgcacgcggt tggccaacgg cgggtcggct agcgccccga ggcccggtgg
     1381 ctggaggtgt aggccacgag ccgtgcccga ctgcgctcga tctcgtcacg aaggagacgg
     1441 tcgagcatcg gcatccacat cgtgccgccg tagtgaaggc gcatggtcag ctcggtgcca
     1501 tccggtcgct cgttcaggtc ggcggtgagc acccactcgg agtgctgccg tccgtcgagt
     1561 tcggcacggt cgaatcgcac ataccggttg atcgtcgcct ccgttcgcac catgcgcagc
     1621 cgcttcgatc ggcgcaatgg accgagctga ccgcgaagat cgatcgacca ggcttcgatc
     1681 ggctcgtcgc tgtcgtccgg ggcgcttgag tcgcctgcca ctccagtcga cgattccctg
     1741 gccgacgatt ctccggtcgt cccggcatcg gtcctcacgg cacccgtcgt cgcggcatca
     1801 cgcacggcgt ctggtggatc gacctcggcg acggcgcgac cgacgatgcc gatccactcc
     1861 ggataggtcg ccaggtcgat gaggaccgga tagagctcct cggctcgaag gtcggtgcgc
     1921 agcgtggcgt tgatatccac gttgttaccg ttcctcctca ggtaccgccg caacggccgc
     1981 gatcgcagca gcgacggccg attccgaacg catcgggcgg tctccccaca ccgtacgaag
     2041 cagcgggaag ggattcaccg gggcaccgcc tgccggatgg atctcgaaat ggaggtgggt
     2101 cgcagacccg ggagcaccgg tatttcccac gtagccgagg accgttcccg gctcgaccac
     2161 cgctccc
//
LOCUS       DAOOMA010000502         2164 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_63595_length_2164_cov_3.735420, whole genome shotgun sequence.
ACCESSION   DAOOMA010000502 DAOOMA010000000
VERSION     DAOOMA010000502.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 2164)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2164)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2164
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63595_length_2164_cov_3.735420"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..397
                     /locus_tag="PLP95_11765"
     CDS             <1..397
                     /locus_tag="PLP95_11765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB46522.1"
                     /translation="EALAVVHPDLVVASGLGLGAEQYGAQAAVDSGVDLVAVLAYPAP
                     DKVWPRASRERFAALAAAATRVITLSPTAPATKQAAGQACGARDRWLTQACDAAIVVW
                     DAKDRNLGRIVSDLEAAEVSCYLITPPTP"
     gene            complement(451..1380)
                     /locus_tag="PLP95_11770"
     CDS             complement(451..1380)
                     /locus_tag="PLP95_11770"
                     /inference="COORDINATES: protein motif:HMM:NF012976.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intradiol ring-cleavage dioxygenase"
                     /protein_id="HPB46523.1"
                     /translation="MIRNGTRRTGHGHVDSANDEHHGDELHDAGLSADLGLFTRRRAS
                     LTLPGTGLAALVGCSGPEADSGAGAGPTSTGSGATSASGGSISTGSGGDATAAADAGS
                     CEVIPEETAGPYPGYGSNGPDVLSESGVVRQDIRSSFGASSTTADGVPVELVLRIVDT
                     KNGCAPLAGAAVYAWHCDAEGRYSMYSDGVEDENFLRGVQESDADGYVRFTSIFPGCY
                     SGRWPHVHFEVYPSVDDAASASNRLRTSQMAFPEDISTEIYGDSRYPSSARNLSQISL
                     DRDMVFRDGVSLQMAAMTGEVASGLTATLTAPV"
     gene            complement(1492..>2164)
                     /locus_tag="PLP95_11775"
     CDS             complement(1492..>2164)
                     /locus_tag="PLP95_11775"
                     /inference="COORDINATES: protein motif:HMM:NF013986.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="BadF/BadG/BcrA/BcrD ATPase family protein"
                     /protein_id="HPB46524.1"
                     /translation="IIAGTGSVAVAADRERRIWRRFGGWDFVLGDEGSGYALGRRALQ
                     ETLLVHEGRSEASALAEAVVQHWGGTDVEGLPDDVHYPTIDKAWIASFAPIVTDLAAA
                     GDLRALEIIDDEVTVLAEAALAAVAVLRGDERSGRTDAVPVGLFGGIAKDPVFQDRFR
                     ALMGERSDVPLRFVIPEHSALVGAAALAMIDRDPQLRAEALDRLDAAVDVAVAAADAA
                     ARGVR"
BASE COUNT          342 a          780 c          713 g          329 t
ORIGIN      
        1 cgaggccctc gccgtggtcc accccgacct cgtcgtcgcg agcggactcg gcctgggagc
       61 ggaacagtac ggtgcccagg ctgcggtcga ttccggcgtc gacctggtcg ccgtgttggc
      121 ctacccggca ccggacaagg tgtggccccg agcgtcgcgg gaacgcttcg ccgcactcgc
      181 cgccgcggcc accagggtga tcacgctgag cccgacggcg ccggccacca agcaggcggc
      241 gggacaggct tgcggcgctc gcgaccgatg gctgacccaa gcctgcgatg cggcgatcgt
      301 ggtgtgggac gcgaaggacc gcaacctggg tcggatcgtg tcggatctgg aggcggccga
      361 ggtgagttgt tatctcatca cgccgccgac cccttgacct cggctctgag gccgctgtcg
      421 ccgctgtcgc cggccgaagc cgcaggtcga ttacaccggt gcggtgagcg tcgccgtcag
      481 ccccgaggcc acctcgccgg tcatcgccgc catctgcaac gacacgccgt ctcgaaagac
      541 catgtcgcgg tcgagtgaga tctgcgagag gttccgagcg ctcgacgggt accggctgtc
      601 gccgtagatc tcggtgctga tgtcctccgg aaacgccatc tgcgaggtcc gcagacgatt
      661 cgatgccgac gccgcgtcgt cgacggaggg gtacacctcg aagtgcacgt ggggccaccg
      721 gcccgaatag cacccgggaa aaatcgatgt gaaacgcacg taaccatcgg cgtcgctctc
      781 ctgcaccccg cgcaggaagt tctcgtcttc caccccgtcg ctgtacatcg agtacctgcc
      841 ttcggcgtcg cagtgccagg cgtagaccgc agctccggcg agcggcgcac atccgttctt
      901 ggtgtcgacg atgcgaagaa ccagttcgac cggcacgccg tccgcggtcg tcgacgacgc
      961 accgaaactc gagcgaatgt cctggcgtac gaccccggat tcgctcaaca cgtcggggcc
     1021 gttcgagcca tacccgggat acggtccggc ggtctcctcg gggatcacct cgcacgaacc
     1081 ggcatcggct gcagccgtcg cgtcgccgcc cgagcccgtc gagatggagc ccccactcgc
     1141 cgaggttgca ccggagccag tcgaggtggg accggcacca gcgccgctgt cggcctcggg
     1201 gccggaacaa ccgacgagtg ccgccagtcc ggttccgggc agggtcagcg atgcccggcg
     1261 tcgggtgaac agcccaagat ctgcggacaa tccggcatcg tggagctcgt cgccgtggtg
     1321 ctcgtcgttc gccgaatcga cgtggccatg gcccgttcgg cgggttccgt ttcgaatcat
     1381 cgtcgtctcc ttcgaggtac gaaaccatcg tgaccgcggt gtcttggaac tccctccgag
     1441 acccagccgg gtcggctcag aatgttcccg accctctcga ccccgtggcc ctcagcgcac
     1501 cccgcgcgcc gccgcgtccg cggcggcgac cgccacatcg acggcggcgt cgagccggtc
     1561 gagcgcctcg gcgcgcagct gcgggtcccg gtcgatcatc gcgagcgccg ccgccccgac
     1621 gagtgcggag tgttcgggga tcacgaatcg caggggtacg tcacttcgtt cgcccatgag
     1681 cgcccgaaag cgatcctgga agaccgggtc cttcgcaatg ccgccgaaca gccccacggg
     1741 aaccgcatcg gtgcggccgg accgctcgtc gccgcgcaac accgcgaccg ccgcgagcgc
     1801 agcttccgcg agcaccgtga cctcatcgtc aatgatctcg agcgcccgca ggtcgcccgc
     1861 cgcggccagg tcggtgacga tgggggcgaa cgatgcgatc cacgccttgt cgatcgtggg
     1921 gtagtgaaca tcgtcgggga gcccttcaac gtcggtcccg ccccaatgtt gcacaaccgc
     1981 ctccgccagg gccgacgcct ccgaacggcc ctcgtgtacg aggagcgtct cctgcaaggc
     2041 tcgacgtccc agcgcgtaac cggacccttc gtcgccgagc acgaagtccc agcctccgaa
     2101 tcgacgccag atccgccgct cgcggtcggc cgcgaccgcg acgctgccag tgccggcgat
     2161 gatc
//
LOCUS       DAOOMA010000503         2151 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_64167_length_2151_cov_3.761927, whole genome shotgun sequence.
ACCESSION   DAOOMA010000503 DAOOMA010000000
VERSION     DAOOMA010000503.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 2151)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2151)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2151
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64167_length_2151_cov_3.761927"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..363)
                     /locus_tag="PLP95_11780"
     CDS             complement(<1..363)
                     /locus_tag="PLP95_11780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl/decaprenyl-phosphate
                     alpha-N-acetylglucosaminyl 1-phosphate transferase"
                     /protein_id="HPB46525.1"
                     /translation="MMRSYLIVLAVSVVVTAAVMPLMMRLSAKFGAIAHPSDRKVHAQ
                     PTPSLGGGAMYLGLLAGFACAALLGEFESVFESPSNIVGVAIAATVIFATGLVDDIRD
                     VSPPAKVAGMVLAGSILAL"
     gene            complement(424..1674)
                     /gene="glyA"
                     /locus_tag="PLP95_11785"
     CDS             complement(424..1674)
                     /gene="glyA"
                     /locus_tag="PLP95_11785"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015799196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HPB46526.1"
                     /translation="MGWPTERDDAVFSLIEREVERQNTGLQLIASENFTSPEVMEATG
                     SVLTNKYSEGYPGKRYYGGNEFVDDIETIAIDRVRELFGAEFANVQPHSGANANLGVY
                     LALLEAGDTVLGMSLDHGGHLTHGSPVNISGRTYNFVPYQVTPSDERIDYDQIRDLAK
                     QHRPKLIVAGATAYPRIIDPAVLREIADESGALLMFDAAHIAGLIAGGAHPNPVPYSD
                     IVTFTTHKTLRGPRGGCILAREQYGAAINKAIFPGLQGGPLEHVIAGKAVAFREAMHP
                     SFTDYANQIVANAQALAAALGAEGFRLVSGGTDNHLMLVDLRPFDAELTGKDAQLVLD
                     EAGITLNKNTIPDDPRSPFVTSGLRIGTPSVTTQGMREAEMAQIAEFIARVLRHRDDR
                     SVIDAVRAEVADLCARFAPYRASR"
     gene            complement(1722..>2151)
                     /gene="rpiB"
                     /locus_tag="PLP95_11790"
     CDS             complement(1722..>2151)
                     /gene="rpiB"
                     /locus_tag="PLP95_11790"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012222566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase B"
                     /protein_id="HPB46527.1"
                     /translation="VGSDHAGFELKNQLADHLRSLGHEVTDLGAHSADRVDYPDFGAA
                     VGRAVAAGEADLGVCVCGSGIGIAIAANKVPGVRAATVHDATSGRLAREHNDANVICI
                     GERLTGPQVALDTLEAFVNASFEGGRHADRVAKLDALLPG"
BASE COUNT          371 a          687 c          742 g          351 t
ORIGIN      
        1 caacgccagg atcgaccccg caagcaccat gccggcaacc ttcgcaggag gcgacacgtc
       61 gcggatgtcg tcgacgagcc cggtcgcgaa aatcacggtc gcagcgatcg cgacaccgac
      121 gatgttcgac ggtgactcga acaccgactc gaactcgccg agcaacgccg cacacgcgaa
      181 ccctgccagg agaccgagat acatcgctcc cccgccgagc gacggagtgg gctgtgcgtg
      241 aaccttgcgg tcgctcggat gggcgatcgc cccgaacttc gcggacagtc gcatcatgag
      301 cggcatcacc gccgccgtga cgacgacact gaccgcgagc acgatcagat atgaccgcat
      361 cacaggatca ccctagcggc gcgatcgtcg acaacccggt gttcgagacc gctcggagcg
      421 cggtcagcgc gacgcccgat acggggcaaa ccgcgcacag agatcggcga cctcggcgcg
      481 aaccgcgtcg atgaccgagc ggtcgtcgcg gtgccgcaac acccgggcga tgaactccgc
      541 gatctgggcc atctcggcct cgcgcatgcc ctgagtggtg accgacggcg tgccgatgcg
      601 cagcccggag gtcacaaacg gcgaacgagg atcgtcgggg atggtgttct tgttgagcgt
      661 gatgccggcc tcatcgagca cgagctgcgc gtccttgccg gtgagttcgg cgtcgaaggg
      721 acgcaggtcg accaacatga ggtgattgtc ggttcctccc gacacgaggc ggaacccctc
      781 ggcgccgagc gcagcggcga gcgcctgtgc gttggccacg atctggttcg catagtcggt
      841 gaaactcggg tgcatggcct cgcgaaaggc cacggcctta ccggcgatga cgtgttcgag
      901 cgggccgccc tgcaacccgg ggaaaatcgc cttgttgatc gccgccccgt actgctcgcg
      961 agcgagaatg cacccgccgc ggggaccgcg aagggtcttg tgggtcgtga aggtcacgat
     1021 gtcgctgtag ggcaccggat tcggatgagc gccgccggcg atcaacccgg cgatatgggc
     1081 ggcgtcgaac atcaacagcg cacccgactc atccgcgatc tcgcgcagca ccgcggggtc
     1141 gatgattctc ggatacgccg tcgcaccggc cacgatgagc ttggggcgat gctgtttcgc
     1201 gaggtcgcgg atctggtcgt agtcgatgcg ttcgtccgac ggggtcacct ggtagggaac
     1261 gaagttgtag gtccggcccg agatgttcac cgggctgccg tgggtgaggt ggccgccgtg
     1321 atcgaggctc atccccaata cggtgtctcc ggcctcgagc agcgcgaggt acacgcccag
     1381 gttcgcgttt gcccccgagt ggggctgaac gttggcgaac tcggcaccga acagttcacg
     1441 cacccggtcg atggcgatgg tctcgatgtc gtcgacgaac tcgttgccgc cgtagtaccg
     1501 cttgcccggg tacccctcgg agtacttgtt ggtgagcacc gaaccggtgg cctccatgac
     1561 ctccggggaa gtgaagttct ccgaggcgat cagctgcagc cccgtgtttt gccgctcgac
     1621 ctctcgttcg atgagggaga agacggcatc gtcgcgttcg gttggccagc ccatggggac
     1681 ctttcgtcgg tggagggagt tcgtttcgtc gacccggcgg ttcagccggg caggagagcg
     1741 tcgagctttg cgacgcgatc ggcatggcga ccgccctcga acgaagcgtt gacgaacgct
     1801 tcgagggtgt cgagtgctac ctggggtccg gtgagtcgct cgccgatgca gatgacattc
     1861 gcgtcgttgt gttcgcgagc cagccgcccc gaggtggcgt cgtgcacggt ggcggcgcga
     1921 acaccgggca ccttgttggc ggcgatggcg atgccgatgc ccgagccgca cacgcacacg
     1981 ccgaggtcgg cctcgcccgc ggcgaccgcc cgcccaacgg cggcgccgaa atcggggtag
     2041 tcgacgcggt cggcgctgtg ggcaccgaga tcggtgacct cgtggccgag ggagcgcaga
     2101 tggtcggcca gctgattctt gagctcgaag cccgcgtgat ccgaaccgac a
//
LOCUS       DAOOMA010000504         2145 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_64413_length_2145_cov_4.058852, whole genome shotgun sequence.
ACCESSION   DAOOMA010000504 DAOOMA010000000
VERSION     DAOOMA010000504.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 2145)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2145)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2145
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64413_length_2145_cov_4.058852"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1604
                     /locus_tag="PLP95_11795"
     CDS             <1..1604
                     /locus_tag="PLP95_11795"
                     /inference="COORDINATES: protein motif:HMM:NF013799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HPB46528.1"
                     /translation="PAAVRAAARAAELSGDTGSAPHRWLLAARSASMAGLDPTVYINR
                     ARTGPIEVRAEAIVLEAAAVSLARRAWVLAEGRFDIDHPFGLMPAIAIAVNAHFGPNG
                     HAELFECRRLVQARAAVDLATPVTMGLLVAGRHEEAFEFAKAMYRQSVRDGRILAAAW
                     TATAAGMASLLMGDLRSTLRWCTLGLELGQAADVPYVIAQATSQLATVAALRGDREAV
                     EELTKAVDTPPLCDSPTPTLHSRRFASILDELQSGSLDLAVEQLRAFRDVAIPMAEHW
                     PIAYELVEALVRLGRNDEALEVMPLVDAIEAERSLQHGGQIERCRALTAGPENFDHHF
                     LVAIECLEGQHRSLELARTRLCFGERLIATGRNSEALEPLRSALATFDNLRTGRAAGL
                     PEEVDTTHIDPAAAAASGFYGFMSRVDHLSLLDTLAGWDRSLGNDLPVLSWCSLAVTD
                     AAGHESGPHGELARAAVRDSDARVGEIVTAVERAGVLERTAFFVIADHGMEQCDPEVV
                     RPWEDDLTEAGVQHRILGEGLIYLS"
     gene            1724..>2145
                     /locus_tag="PLP95_11800"
     CDS             1724..>2145
                     /locus_tag="PLP95_11800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB46529.1"
                     /translation="MMPSPHPTLRHLAVAVTVVFAAGAAMLGCSNAGDQHADDTGASS
                     PTTAPSTSSPSSPSALERPYGVGLVEEVFVDDTRPTSGAAGRAEQPNRTVETTILYPA
                     NVGSDGGIADGAEPADRGGPFPLVVLGHGLGGNVEYLTP"
BASE COUNT          336 a          752 c          720 g          337 t
ORIGIN      
        1 ccccggcggc cgtccgggcg gcggcacggg ccgcggagct ctcgggcgac accggctcgg
       61 cgccacaccg ctggttgctc gcagcgagaa gcgcgtcgat ggccgggctc gacccgaccg
      121 tgtacatcaa ccgggcacgg acggggccga tcgaggtcag agccgaggcg atcgtcctcg
      181 aagcggcggc cgtgtcgctg gcgaggcgcg cctgggtcct cgcagagggg cgattcgaca
      241 tcgaccaccc gtttggcctg atgccggcga tcgccatcgc ggtcaatgcc catttcggcc
      301 ccaacggtca cgccgaactg ttcgagtgtc gccggttggt gcaagctcgg gccgccgtcg
      361 atctggcgac cccggtgacg atgggcctcc tcgtcgccgg ccgacacgag gaggcgttcg
      421 agttcgccaa ggcgatgtac cgacaatcgg tacgcgacgg aaggatcctc gccgcagcat
      481 ggacggcgac ggccgccggt atggccagcc tgctgatggg agaccttcgg tcgacgctgc
      541 gctggtgcac gctcggcctt gagctcggcc aggcggccga cgtgccctat gtgatcgctc
      601 aggcgaccag ccaactggcc acggttgcgg cgttgcgcgg cgaccgggag gccgtcgagg
      661 aactcaccaa ggcggtcgac actccgccgc tgtgcgactc gccgaccccc acactgcact
      721 cgcgacggtt cgcctccatc ctcgacgagc tccagtccgg ctccttggac ctggcggtcg
      781 agcagctacg tgcgtttcgt gacgtggcaa tcccgatggc cgagcactgg ccgattgcct
      841 acgagctcgt cgaggcactg gttcgcctcg gccgcaacga cgaggccctc gaggtgatgc
      901 ccctcgtcga tgccatcgag gccgaacggt cgcttcagca cggcggccag atcgagcgat
      961 gccgtgcgct caccgcgggg ccggagaact tcgaccatca cttcctcgtc gcgatcgaat
     1021 gtctcgaggg ccagcaccgt agccttgagc ttgcacgcac ccgcctgtgc tttggcgagc
     1081 ggctgatcgc caccgggcgc aactccgagg cgctcgaacc gcttcgctcc gcgttggcga
     1141 ccttcgacaa cctccgtacc ggtcgggcag cggggcttcc cgaggaggtc gacaccaccc
     1201 acatcgaccc cgctgcggca gcggcatcgg gtttctacgg cttcatgtcg cgggtcgacc
     1261 atctcagcct gctcgacacg ctcgctggtt gggaccgcag cctcggcaac gatttaccgg
     1321 tgttgagctg gtgcagcttg gcggtcaccg acgctgcggg ccacgagagc ggcccgcacg
     1381 gcgaactcgc acgcgctgca gttcgcgaca gcgacgcgcg cgtcggagag atcgtgaccg
     1441 cggtcgagcg cgcaggggtg ttggaaagga ccgcgttctt cgtcatcgcc gatcacggaa
     1501 tggaacagtg cgaccccgaa gttgtgcgcc cctgggagga cgatctcacc gaagcggggg
     1561 tacaacatcg catcctcggt gaagggctca tctacctgag ctagtgccgt gtcaagactg
     1621 ccgggccgac tcaggggttc agattgagtt cgagcagtgc cgggccgacg aggcggatcg
     1681 acgcagctat cgtcgccaac gtgcccacca aggaccaggc ctcgtgatgc cttcacccca
     1741 tccaaccctg cgccacctcg ccgtcgccgt cactgtcgtg tttgccgccg gcgcagcgat
     1801 gctcggatgc agtaacgcag gagaccagca cgccgacgac accggcgcat cgagccccac
     1861 gaccgccccg tcgacatcct cgccgtcgtc gccgtcggcc ttggagcgcc cctatggcgt
     1921 cggcctggtc gaggaggtat tcgtcgacga cacccggccg acatcggggg ccgcgggtcg
     1981 cgccgagcag cccaaccgca ccgtcgagac caccatcttg tacccagcca acgttggctc
     2041 tgatgggggg atcgccgatg gcgccgagcc ggccgaccgc ggcggaccgt tccctctggt
     2101 cgtgctcggc catgggctcg ggggcaacgt ggagtacctg acccc
//
LOCUS       DAOOMA010000505         2141 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_64582_length_2141_cov_4.334612, whole genome shotgun sequence.
ACCESSION   DAOOMA010000505 DAOOMA010000000
VERSION     DAOOMA010000505.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 2141)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2141)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2141
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64582_length_2141_cov_4.334612"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1078
                     /locus_tag="PLP95_11805"
     CDS             <1..1078
                     /locus_tag="PLP95_11805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HPB46530.1"
                     /translation="GFGNPDLASPDVAVDKLVEAAQNSRNHRYSSSRGLPKLREAIAG
                     YYLRRFGVELDPDRHVINTIGAKEGFSHLMWTLLGPGDAALVPSPSYPIHIYGPLLAG
                     ADVRELPMINGAEQFFDAMVDTWKYSFPKPRVIVLSFPHNPTTTCVDLDFFQRVVDFA
                     RENDVVIVHDNAYADLGFDGYQPPSILQAEGALECCVEFYSMTKSFSMAGWRVAFMLG
                     NAEVVSALQKLKSYLDYGTFQPIQIAATVAINEAPDFPAEQTAIYQSRRDALCDGLNR
                     IGWEMEPPRGTMFAWAPIPEAYRDLGSIEFAKLLVREGQVAVSPGVGFGPDGDGHVRF
                     ALIENEQRTAQAIRNLRRGLTRLG"
     gene            complement(1075..1713)
                     /locus_tag="PLP95_11810"
     CDS             complement(1075..1713)
                     /locus_tag="PLP95_11810"
                     /inference="COORDINATES: protein motif:HMM:NF036525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HPB46531.1"
                     /translation="MSDDTDGGADGGATPTRRLTPRGSQRRSQLIEFATRKFASDGYH
                     PTSVADIVDGIGVGKGVFYWYFESKEMLLEQILRDAQRGLRQRQREAASAAPDPIEKL
                     EFGIRAAVRWTAEHEDLAKLVEFAQTDERFEPLVRRGRAALVGDTVPLVRKAMKQGVI
                     PDGDAEMLSYAIFGVASSLSAALLPREGVDPDHIADAVVEFCLRGIGARPDG"
     gene            1826..>2141
                     /locus_tag="PLP95_11815"
     CDS             1826..>2141
                     /locus_tag="PLP95_11815"
                     /inference="COORDINATES: protein motif:HMM:NF013962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HPB46532.1"
                     /translation="MPLFVVRVWLPDRPGALGAVASRIGSVRADVVGIDILERGAGRA
                     VDELTIDIEDPQHVDLLIKEIGQVDGVDVEHVRPARAVLGDRAVEALDLAAAIASATD
                     REE"
BASE COUNT          344 a          707 c          702 g          388 t
ORIGIN      
        1 ggggttcggg aatccggacc tcgcgtcgcc cgacgtcgcg gtcgacaagc tggtcgaggc
       61 ggcgcagaac agccggaacc atcggtattc ctcgagccgc ggcctgccca agctccgaga
      121 ggcgatcgcc gggtactacc tgcgtcgttt cggggtcgaa ctcgaccccg atcgccatgt
      181 gatcaacacc atcggggcga aggaaggctt cagccacctg atgtggaccc tgctcggccc
      241 cggcgacgcg gcgctggtgc cgtcgccgag ttacccgatc cacatctacg gtccgcttct
      301 cgccggcgcc gacgtgcgcg agttgccgat gatcaacggt gccgagcagt tcttcgatgc
      361 gatggtcgat acctggaagt acagctttcc gaagccccgg gtcatcgtgt tgtccttccc
      421 gcacaacccg acgacgacct gtgtcgacct cgacttcttt caacgcgtcg tcgacttcgc
      481 ccgcgaaaac gacgtggtca tcgtgcacga caacgcgtat gcagacctcg ggttcgacgg
      541 ttaccagccc ccgtcgatcc tgcaggccga gggggcgctc gagtgctgcg tcgagttcta
      601 ttcgatgacg aagtcgttct cgatggccgg gtggcgtgtc gccttcatgc tgggcaacgc
      661 tgaggtcgtc tcagcgctgc agaagctcaa gagctacctc gactacggca cgtttcagcc
      721 catccagatc gccgctacgg tggcgatcaa cgaggccccc gatttccccg ccgagcaaac
      781 ggccatctac cagagccgtc gcgacgcgct ctgcgacggt ctcaaccgga tcgggtggga
      841 gatggagccg cctcgcggaa ccatgttcgc gtgggcgccg attccggagg cctatcgaga
      901 tctcggttcg atcgagttcg cgaagctcct cgtgcgtgag ggtcaggtgg cggtctcgcc
      961 cggcgtcgga ttcggcccgg atggcgatgg ccacgtccgc ttcgccctca tcgagaacga
     1021 acagcgaacc gcccaggcga tccgcaacct tcgtcgcgga ctcaccaggc tcggttagcc
     1081 gtccggccgg gcaccgattc cccgaaggca gaactcgaca accgcatcgg cgatgtggtc
     1141 ggggtcgacg ccctcgcgcg gcagcagcgc cgcgctgagg cttgaggcga cgccgaagat
     1201 cgcataggag agcatttcgg cgtcgccgtc ggggatgacc ccctgcttca tcgcctttcg
     1261 gacgagggga acggtgtcgc cgacgagcgc agcgcgcccc cggcgaacga gcggttcaaa
     1321 ccgttcgtcg gtttgggcga actcgacgag ctttgccagg tcctcgtgct cggcggtcca
     1381 tcgaacggcc gcccgaatgc cgaactcgag cttctcgatg gggtccggtg cggcgctggc
     1441 ggcctctcgt tggcgctggc gcagtcctcg ctgagcgtcg cgcaggatct gctcgaggag
     1501 catctccttg gactcgaagt accagtagaa cacgcccttg ccgacgccga tcccgtcgac
     1561 gatgtcggct accgacgtcg ggtggtagcc gtcggatgcg aacttgcgag tggcgaattc
     1621 gatcagttgg ctgcgccgct gagaaccccg cggggtgagc cgacgtgttg gcgtggcgcc
     1681 tccgtcggcg cctccgtcgg tgtcgtcgct catgagcgga caagctacca gtcttgaccg
     1741 ggcggtcaag accgtcggcg gcggagttca cacgcccgta atggcgctac acagtgcgtt
     1801 catgccgcct cgataacctg caggcatgcc gttattcgtt gttcgtgttt ggcttcctga
     1861 tcgcccgggc gcactcggcg cggtcgcgag tcgcatcggc tcggtccgcg ccgatgtggt
     1921 cggtatcgac atcctcgagc gcggcgctgg tcgggcggtc gatgagttga cgatcgacat
     1981 cgaagaccca cagcacgtcg acttgctcat caaagagatc ggccaggtcg acggcgtcga
     2041 cgtcgaacat gttcggcccg cccgtgcggt gctgggcgac cgcgcggtcg aggcactcga
     2101 tctcgccgcc gcaatcgcct ccgccaccga tcgcgaggag c
//
LOCUS       DAOOMA010000506         2140 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_64631_length_2140_cov_3.426379, whole genome shotgun sequence.
ACCESSION   DAOOMA010000506 DAOOMA010000000
VERSION     DAOOMA010000506.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 2140)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2140)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2140
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64631_length_2140_cov_3.426379"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..596)
                     /locus_tag="PLP95_11820"
     CDS             complement(<1..596)
                     /locus_tag="PLP95_11820"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HPB46533.1"
                     /translation="MTNHFDLCIIGSGSGNTIVDDRFDGWKVAIVERGVYGGTCLNVG
                     CIPTKMLVYPADLVDDARDAARFGVDLDVGGVRWSDIRDRVFGRIDPISASGLEYRKD
                     LPNVTVFEANAAFVGHKRLRVGTEEITADRFVIAAGARSVVPEISGLAEVPFDTSDTI
                     MRIDEVPEHLVVIGGGFIALELAHVFEAYGATVTVLARG"
     gene            complement(627..>2140)
                     /locus_tag="PLP95_11825"
     CDS             complement(627..>2140)
                     /locus_tag="PLP95_11825"
                     /inference="COORDINATES: protein motif:HMM:NF015651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HPB46534.1"
                     /translation="PFAGLRELAEHEALVSLKAEAGGVPTPTLEAIGEVDEGGGYLLA
                     FRDSDTTPFSEVAEPGDDALTGLWNLLVRLRWARIAHQGLDQNAVVVGPDGDMQVINF
                     GVADLTGDERVLSGDIAELLAWTSLRFSPERTVNAAIAALGPGVVARSLSRLQPLALT
                     RSTRATIDSGTLESIAELIRERTGTDQQALAPLERIKPRHVLMFVMGVLAINALAPQL
                     AGFPEVWKELQNADLIWVLAVALASATSYIFAAFGLSGSLAEPLPAGPNVALQVAGSF
                     VGIAAPAQLGGVALQGRFLQRRGIDPAVVVAAIGLNQIAAFATHVIILIGFVWWAGSD
                     ELSQISLPSWATIGIGAGAVAVLAAVFLALPFGRRVVRETLVPALRRSAQGIVEVAKR
                     PVRLVGLLGGTAGVTLSYTAALVAAVKCFYGDVPFAPVAIVYLLGSFVQAVAPTPGGL
                     GAAEVAYIGGLTAIGVPSEQAVAAVLVYRFFTFFAPVLPGWFAMLWLQRTEAI"
BASE COUNT          406 a          721 c          711 g          302 t
ORIGIN      
        1 ccgcgggcca acacggtcac cgtcgctccg tacgcctcga acacatgggc cagttcgagg
       61 gcgatgaacc cgccgccgat gaccacgagg tgttcgggca cctcatcgat gcgcatgatc
      121 gtgtcggagg tatcgaacgg aacctcggcg aggccggaaa tctcgggcac gacgctgcgg
      181 gccccggcgg cgatcacgaa gcggtctgcg gtgatctcct cggtgccgac acgaagacgc
      241 ttgtggccga cgaacgccgc gttcgcctcg aacaccgtga cgttcggcag gtccttgcgg
      301 tactcgagcc ccgaggcgga aatcgggtcg atgcggccaa agacccgatc ccggatgtcg
      361 ctccagcgga cgcctccgac atccaggtcg acgccgaacc gagcggcgtc tcgggcgtcg
      421 tcgaccaggt cggcggggta gaccagcatc ttggtcggga tacagccgac attgagacag
      481 gtacctccgt acacaccccg ctcgacgatc gccaccttcc agccgtcgaa tcggtcgtcg
      541 acgatggtgt tacccgaccc ggacccgatg atgcagagat cgaagtggtt cgtcacccag
      601 gcaatgttac gaccaccccg cagggctcag atcgcctcgg tgcgttgcag ccacaacatg
      661 gcgaaccagc cgggcaggac cggagcgaag aacgtgaaga accggtagac caacacggcc
      721 gcgaccgctt gttccgacgg cacgccgatg gcggtcagcc caccgatgta ggcgacttcg
      781 gcggccccca gaccacccgg agtcggggcg accgcctgaa cgaaactgcc gagcaggtag
      841 acgatcgcca cgggggcgaa gggcacgtcg ccgtagaagc acttgaccgc ggcgactagc
      901 gctgcggtgt agctcagcgt cacgccggcg gttccgccca acagcccgac caggcgcacc
      961 ggacgtttcg ctacctcgac gatgccttgg gcggaacgcc gcagggctgg cacgagggtc
     1021 tcgcgcacca cgcgccgccc gaacggcaaa gcgaggaaaa cggccgcaag cacggcgacc
     1081 gcaccggcac cgataccgat cgtcgcccac gacggcaagc tgatctggct gagttcgtcg
     1141 ctgcccgccc accacacgaa cccgatcagg atgatcacgt gcgttgcgaa ggcggcgatc
     1201 tgattgaggc cgatggcggc gaccaccacc gccgggtcga tccctcgacg ctgcaggaac
     1261 cgaccctgca acgcaacgcc tccgagttga gcgggtgccg cgattccgac gaacgatccg
     1321 gccacctgca acgccacgtt cggacccgcc ggcagcggct cggcgagcga accgctcagg
     1381 ccgaacgcgg cgaaaatata tgacgttgcg ctggccagcg cgacggcgag cacccagatg
     1441 aggtcggcgt tctgcagctc cttccacacc tcgggaaatc cggcgagttg cggggccaag
     1501 gcgttgatcg ccagcacgcc catgacgaac atgaggacgt ggcggggctt gatccgttcg
     1561 agcggggcga gcgcctgttg atcggtgccg gttcgttccc ggatgagttc ggcgatcgac
     1621 tccagggttc ccgaatcgat cgtggcgcgg gtggaccggg tgagggccaa cggctgcagc
     1681 cgcgacagcg aacgcgccac gactccgggc ccgagggcgg cgatggcagc attgactgtt
     1741 cgctcggggg agaaccgcaa cgaggtccag gccaacagct ccgcaatgtc gccgctgagc
     1801 acgcgttcgt ctccggtgag gtcggccaca ccgaagttga tgacctgcat gtcgccatca
     1861 gggccgacga cgaccgcgtt ctggtcgagg ccctggtggg cgatacgcgc ccagcggaga
     1921 cgcacgagaa gattccagag gccggtgagg gcatcgtctc cgggttcggc cacctcggaa
     1981 aacggtgtgg tatcggaatc tcgaaacgcc aggaggtagc cccctccctc gtcgacctcg
     2041 ccgatcgcct ccagcgtcgg cgtgggaact ccaccggctt ctgccttcaa cgacaccagg
     2101 gcctcatgtt cggcgagttc gcgcaggccg gcgaacgggc
//
LOCUS       DAOOMA010000507         2135 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_64838_length_2135_cov_4.425000, whole genome shotgun sequence.
ACCESSION   DAOOMA010000507 DAOOMA010000000
VERSION     DAOOMA010000507.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 2135)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2135)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2135
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64838_length_2135_cov_4.425000"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..943)
                     /locus_tag="PLP95_11830"
     CDS             complement(<1..943)
                     /locus_tag="PLP95_11830"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014906.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HPB46535.1"
                     /translation="MAHDSTSSNVSLSRERIVEEALALLDEEGEEALSMRRLAQRLGA
                     STMSTYHHVSDKETLLDLVTDRVMSELRLPSHDASWQDAVRTMAWSFRSLTHQHPAVF
                     RLVLARPRPAGMLVTSANVRTVLIDRGLAPEFASTVFRCTVRFLLGSTLGEVGGFGGL
                     GIDHHSEEAHQVFESGIEALISGFTQQLGTVRLRAAVPDDRDTIVDVVTRAYRGRGDD
                     AGWTTESHLLGGQRTDPTEVDAAISAHDAVLIVAVDTADNILGCVRVDRSDGSERDGA
                     HFGLFAVDPERQSGGIGALLLGAAEAQAREWHCPSLEL"
     gene            1027..>2135
                     /locus_tag="PLP95_11835"
     CDS             1027..>2135
                     /locus_tag="PLP95_11835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012223290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HPB46536.1"
                     /translation="MAESHDHDHDVNDVSWIMEEYSRRDLDEIAHAVDVNADALFTWD
                     YDRSRPALSKLYEKAKTSQWNGATDLPWEIEVDQEKVATMLVSGNVERFKALSSLPGS
                     PVKNFGDAEWLQVGIEMQNWQLSQFLHGEQGALMCTGLITATTPWIDAKYYAATQVMD
                     EARHVEVFARYLYEKLNSAYPMNANLGNLIDDTIRDSRWDITYLGMQIMVEGLALAAF
                     GAIHMVTPEPLLKQLLRYVMSDEARHVAFGVLSLQEYYQGLNAAELRERQEFAFEAAE
                     RLRNRFFMIEVWERMGVEAAPLIKLMLDNPPPERNEFQHMLFSKIVPNCKKLGLLDAG
                     DGWLRTKFEELGIIQYENWTDTSDEYEQFALENLPA"
BASE COUNT          390 a          653 c          713 g          379 t
ORIGIN      
        1 cgagttccag cgatggacag tgccactcgc gggcctgagc ctcggcggca ccgaggagga
       61 gggcgccgat tccgccgctc tggcgttcgg ggtcgacggc gaacaggccg aagtgcgcgc
      121 cgtcgcgctc gctgccgtcg ctgcgatcga cgcggacaca ccccaagatg ttgtcagcgg
      181 tatccacggc gacaatcagc accgcgtcgt gggcggagat cgccgcatcg acctcggtgg
      241 gatcggtccg ttgcccgccg agcaggtggg attcggtggt ccagccggcg tcgtcgccac
      301 gcccgcgata agcccgggtc acgacgtcga cgatggtgtc gcgatcgtcg gggactgcgg
      361 cgcggagtcg aacggtaccc aactgctgag tgaacccgga gatgagcgct tcgatgccgg
      421 attcaaacac ctggtgtgct tcctcgctgt ggtggtcgat gcccagcccg ccgaaccctc
      481 cgacctcgcc gagggtcgac cccagcagga accgcacggt gcagcgaaac acggtcgagg
      541 cgaattcggg cgccaacccc cggtcgatca gcacggtgcg gacattcgcc gaggtgacga
      601 gcatgcccgc cggccgcggg cgtgccagaa ccaggcgaaa taccgctggg tgctggtggg
      661 tgagcgagcg aaacgaccag gccatcgtcc gcacggcgtc ttgccagctg gcgtcgtggc
      721 tggggagacg cagttcgctc atcacccggt cggtgacgag gtcgagcaac gtttccttgt
      781 cgctgacgtg gtgataggtc gacatggtcg aggccccgag gcgttgggcc agccttcgca
      841 tgctcaacgc ctcttcgccc tcctcatcga gtagggcgag ggcctcctcg acgatgcgtt
      901 cacgcgagag tgagacgttg ctgctggtgg agtcgtgtgc catcggatgt catcgtacgg
      961 cgtacgagct atgctgaagt gattcgtttg tacgccgtac gaattcgccg atcagagggg
     1021 gctgcgatgg ctgagagcca tgaccatgac cacgatgtca acgatgtcag ttggatcatg
     1081 gaggagtact cccgtcgcga cctcgacgag atcgctcacg ctgtcgacgt caatgccgac
     1141 gcgttgttca cctgggatta cgaccgttcg cgtcctgcgc tgtcgaagct gtacgagaag
     1201 gcgaagacct cgcagtggaa cggggccacc gacctgccgt gggagatcga ggtcgaccag
     1261 gagaaggtcg ccacgatgtt ggtgtcgggc aacgtcgagc gcttcaaggc gttgtcgtcg
     1321 ctgccgggtt cgccggtgaa gaactttggt gacgccgagt ggttgcaggt cggtatcgag
     1381 atgcagaact ggcagctcag ccagttcctg cacggtgagc agggtgcgct gatgtgtacc
     1441 ggtctcatca ccgcgacgac gccgtggatc gacgccaagt actacgccgc cacccaggtg
     1501 atggacgaag ctcgccacgt cgaggtgttc gcccggtacc tctatgagaa gctcaacagt
     1561 gcgtatccga tgaacgcgaa tctcggaaac ctcatcgacg acacgatccg cgactcgcgc
     1621 tgggacatca cctatctcgg catgcagatc atggtcgagg ggctcgccct cgcggcgttc
     1681 ggcgccatcc acatggtgac ccccgagccg ttgctcaagc agcttttgcg ctacgtgatg
     1741 agcgacgagg cccgccacgt ggcgttcggg gtgttgagcc tgcaggagta ctaccagggc
     1801 ctcaacgccg ccgaactgcg cgagcgtcag gagttcgcgt tcgaggcggc ggagcgtctg
     1861 cgcaaccgct tcttcatgat cgaagtctgg gagcgcatgg gggtcgaggc ggcgccgctc
     1921 atcaagttga tgctcgacaa cccgccgccc gagcgcaacg agtttcaaca catgctgttt
     1981 tcgaagatcg tgccgaactg caagaagctc ggtctcctcg acgccggcga cgggtggctg
     2041 cgtacgaagt tcgaagaact cggcatcatc cagtacgaga actggaccga cacctctgac
     2101 gagtacgagc agttcgccct cgagaacctt cccgc
//
LOCUS       DAOOMA010000508         2134 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_64888_length_2134_cov_4.193362, whole genome shotgun sequence.
ACCESSION   DAOOMA010000508 DAOOMA010000000
VERSION     DAOOMA010000508.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 2134)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2134)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2134
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64888_length_2134_cov_4.193362"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..795)
                     /gene="ruvB"
                     /locus_tag="PLP95_11840"
     CDS             complement(<1..795)
                     /gene="ruvB"
                     /locus_tag="PLP95_11840"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015798632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HPB46537.1"
                     /translation="MRDELLNPDYTDQDAADEVTDSLDALVGAGDASQAALDAAALRP
                     RTLAEFVGQAELKKRLNIILEAARRRGQAADHLLFAGPPGLGKTTLAGIIANEMDVQL
                     HVTSGPTLERAGDLAAILTKLGEGDVLFIDEIHRLSRTVEEVLYPAVEDFQLDIVLGK
                     GPAARSIRLDLPRFTLVGATTRTGLITGPLRDRFGLVARLDFYETRDIENIVTRAAHI
                     LGARIEGRGAHEIARRSRGTPRIANRLLRRVRDFAEVEADSAITTQV"
     gene            complement(818..1447)
                     /gene="ruvA"
                     /locus_tag="PLP95_11845"
     CDS             complement(818..1447)
                     /gene="ruvA"
                     /locus_tag="PLP95_11845"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HPB46538.1"
                     /translation="MIGSLRGVLLDRQGTEVVIEVGGIGYRLATTPATSGALGAEGAE
                     AFVWVHHHIREEAQTLYGFHTREERDTFETLIGTHGVGPSLGLAILSTLSPYSLRLAL
                     ATDDLASLCTVPGVGKKTAQRLLVELKSRLELGDIDLRAVGGEDGSAAGATPGIGADA
                     GPAAHHDVREALSGLGYSTDEVARALKTLPLEGDASTLLRQALQALAAS"
     gene            complement(1444..1959)
                     /gene="ruvC"
                     /locus_tag="PLP95_11850"
     CDS             complement(1444..1959)
                     /gene="ruvC"
                     /locus_tag="PLP95_11850"
                     /inference="COORDINATES: protein motif:HMM:TIGR00228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction
                     endodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HPB46539.1"
                     /translation="MFVLGIDPGLSRCGYCVLTPKGRSGAAVAAAIGVIRTPPDMATP
                     RRLAMLQRDVIGLLDEFPPVAVAIERVLFQNNVSTAIPVGQASGIIMAEAANRGCDVV
                     EYSPNQVKEAVAGYGSAGKEQVQEMVRRLLGHDKVLRPADAADAAAVALCHIAYNPDP
                     ARPNAAREAIR"
BASE COUNT          330 a          704 c          722 g          378 t
ORIGIN      
        1 gacctgggtc gtgatcgcgc tgtcggcctc gacctcggcg aaatcgcgaa cccgtcgcag
       61 cagtctgttg gcgatacgcg gtgttccccg actgcggcgg gcgatctcgt gcgctccccg
      121 gccctcgatg cgggcaccca ggatgtgagc ggcgcgggtg acgatgttct cgatgtccct
      181 cgtctcgtag aaatcgagcc gggcgacaag gccgaagcga tcgcgtaggg gcccggtgat
      241 cagcccggtg cgggtggtgg cgccgacgag ggtgaaccgc ggcaggtcga gacggatcga
      301 gcgggcagcc gggcccttgc cgagcacgat gtcgagttgg aagtcctcga cggccgggta
      361 cagcacctcc tcgacggttc ggctgagacg gtgaatctcg tcgatgaaca agacatcgcc
      421 ctcccccaac ttggtgagga ttgcggcgag atcacctgca cgctcgagcg tcgggccgga
      481 ggtcacgtgc aactgcacgt ccatctcgtt ggcgatgatg cccgccaacg tcgtctttcc
      541 gagacccggt gggccggcga agaggaggtg gtcggccgcc tgcccgcgcc ggcgcgccgc
      601 ctcgaggatg atgttcagac gtttcttgag ctccgcctgg ccgacgaact cggccagtgt
      661 ccgcggccgc aatgctgccg cgtcgagcgc tgcctgtgag gcgtcgcctg ccccgacgag
      721 cgcgtcgagt gaatcggtca cctcatcggc agcgtcctgg tcggtgtagt cggggttcag
      781 cagttcgtcg cgcatcgtgt cgcctcttca gcctggatca gctcgccgcc agcgcctgga
      841 gggcttggcg cagcagggtc gatgcgtcgc cctcgagtgg caacgtcttg agtgctctgg
      901 ccacctcgtc ggtggaatat ccgaggcccg acaatgcctc gcggacgtcg tgatgcgccg
      961 ccggaccggc gtcagcgcca attccaggcg tggctcccgc ggccgaaccg tcctcgcctc
     1021 ccacagctcg caggtcgatg tcgccgagtt cgaggcgcga cttcagttcg acgagcagcc
     1081 gctgcgcggt cttcttgcca actcccggca cggtgcacag cgatgccagg tcgtcggttg
     1141 cgagcgcgag ccgcagcgaa tacggcgaga gggtcgacaa gatggcgagc ccgagcgacg
     1201 gtcccacacc gtgggtgccg atgagcgttt caaacgtgtc gcgttcctcg cgtgtgtgga
     1261 acccgtacag ggtttgggcc tcctcgcgga tgtggtggtg gacccatacg aacgcctcgg
     1321 ccccctcggc gccgagcgca cccgaggtcg ccggggtggt cgcaagccga tacccgatgc
     1381 cgccgacctc gatgaccacc tcggtgccct gccggtcgag cagcacgcca cgcaacgacc
     1441 cgatcatcga atcgcctcac gtgctgcgtt cggccgggcg gggtcagggt tgtaggcgat
     1501 gtgacacaac gccaccgcag cggcatctgc ggcgtcggcc ggacgcaaca ccttgtcgtg
     1561 gccgagcagg cggcggacca tctcctgtac ctgttccttt ccagccgacc catagccggc
     1621 gaccgcttct ttgacctggt tgggcgagta ttcgaccacg tcgcagccac gattcgcggc
     1681 ctcagccatg atgatgccac tcgcctgccc gacggggatc gccgtcgaga cgttgttttg
     1741 aaacagcacc cgctcgatcg ccacggcaac cggcggaaac tcgtcgagca ggccgatgac
     1801 gtcgcgctgc agcatcgcga gacgccgggg cgtcgccatg tccgggggcg ttcgaatgac
     1861 accgatcgcc gcggcgaccg ccgctccact tcgccccttg ggggtgagca cgcagtagcc
     1921 gcaacgcgac aatccgggat cgattccaag cacgaacacg cgtacgacac tagccgcgga
     1981 tggctgcacg gtcgcgtttc gccctcttca ggatcgtcag cgctgatgaa tacggccgtt
     2041 ctggcgtggg atccggggct tccgtttcta ggtttggctg tctcggggct tcgttgtcgg
     2101 gggttccgtt tagtatcaaa cgcatgttcg aggg
//
LOCUS       DAOOMA010000509         2134 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_64895_length_2134_cov_3.717653, whole genome shotgun sequence.
ACCESSION   DAOOMA010000509 DAOOMA010000000
VERSION     DAOOMA010000509.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 2134)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2134)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2134
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64895_length_2134_cov_3.717653"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1413)
                     /locus_tag="PLP95_11855"
     CDS             complement(<1..1413)
                     /locus_tag="PLP95_11855"
                     /inference="COORDINATES: protein motif:HMM:NF012377.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase family glycosylhydrolase"
                     /protein_id="HPB46540.1"
                     /translation="MRRGATPTTLVRTAAVVLVAVLSASCSDSDGADEAAGGASTTNS
                     ASVTTVPVDGDLVGAGGPLELLPLQTAGTRIVDTAGRDVLLRGANLNSLGEYWQGVPE
                     VDATVEVTADDWDLMAANGFSVVRLIVSWSRIEPERGEFDDAYLDEVEAAVDEAAARG
                     IYTVVDMHQDAFTASLWTEDPSTCPEGTVPAKGWDGAPGWAVTTDGLSTCLRNGDRNS
                     SPAVEAAWNHFYDNTDGTRDHFVAAWAAVAERFAGNAAVAGYDLLNEPEVSRPTAELQ
                     PVYDEFMAQTIESIRTAEREAGAPFEHIVFVEPAIPAGDLSRGIVVPTEATFAGGTTN
                     AAVSVHNYMESINHIISLESLNESTQAMADALGVASWGGEYGFWDVEPDTLAVGRRYA
                     ADEDAHRWGGAWWQWRQSCGDPHSVQWENGAVVADAETEVHLNLLGCPGNEPLGPNEE
                     FLSIVGRSFPRAAPGTIVELR"
     gene            1852..>2134
                     /locus_tag="PLP95_11860"
     CDS             1852..>2134
                     /locus_tag="PLP95_11860"
                     /inference="COORDINATES: protein motif:HMM:NF015338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HPB46541.1"
                     /translation="MALSIVTANVNGIRAALKRDMESWVSAAAPDIMALQEVRAPDAL
                     VRDVIEELCEGGWHVVHSEAAAKGRSGVAVVSRVPIVGTIDAEVAGFSAR"
BASE COUNT          367 a          744 c          686 g          337 t
ORIGIN      
        1 tcgcagctcg acgatggtgc cgggagccgc ccgcgggaac gaccgtccga caatgctcag
       61 gaattcctcg ttgggaccga ggggctcgtt gccggggcag cccagcaggt tcagatgcac
      121 ctcagtttcg gcatcggcga cgaccgcacc gttctcccac tgcaccgaat gtggatcgcc
      181 acacgactgg cgccactgcc accacgctcc accccaccgg tgcgcgtcct catccgcggc
      241 gtaacgccgg ccgaccgcca aggtgtcggg ttcgacgtcc cagaacccgt actcgccgcc
      301 ccaactcgcc accccgagtg cgtcagccat cgcctgggtg gattcgttca acgattcgag
      361 cgaaatgatg tgattgatcg attccatgta gttgtgcacc gaaaccgcgg cgttggtcgt
      421 gccacccgcg aaggtcgcct cggtgggcac cacgatgccg cggctcaggt cacctgccgg
      481 gatcgccggc tcgacgaaga cgatgtgctc gaacggggcg ccggcctcgc gttcggccgt
      541 gcggatcgac tcgatcgtct gcgccatgaa ctcgtcgtag accggctgca attcggcggt
      601 ggggcgggat acctcgggtt cgttcaacag gtcgtagccg gcgaccgcgg cgttgccggc
      661 aaaacgctcg gcgaccgctg cccacgctgc gacgaagtgg tcgcgggtgc catcggtgtt
      721 gtcgtagaag tgattccacg ccgcctcgac cgctggcgac gaattccggt cgccgttgcg
      781 cagacacgtc gacaggccgt cggtggtgac ggcccaaccc ggcgcaccgt cccaaccctt
      841 cgccgggacc gtcccctcgg ggcatgtcga ggggtcctcg gtccacagcg atgcggtgaa
      901 ggcgtcctgg tgcatgtcga cgaccgtgta gatcccccga gctgccgcct catcgaccgc
      961 cgcttcgacc tcgtcgaggt aggcgtcgtc gaactccccg cgttcgggtt cgatacgcga
     1021 ccaactcacg atcaaccgca cgacactgaa cccgttggcc gccatcagat cccagtcgtc
     1081 cgcggtgacc tcgacggtgg catcgacctc aggcaccccc tgccagtact cgccgagcga
     1141 gttcagattc gccccgcgca acaacacgtc gcggccagcg gtgtcgacga tgcgggtgcc
     1201 cgccgtctga agcggcaaca actcgagcgg accgcccgca ccgacgagat cgccgtcgac
     1261 cggaaccgtc gtcaccgacg cgctgttggt cgtcgatgcc ccgccagcgg cctcatcagc
     1321 gccgtcggag tccgaacagg aagcggacaa caccgcgacg agaacgaccg cagccgttcg
     1381 tacaagcgtc gtcggtgtgg cgcccctcct catggcgccg agactaccga ccgacaccac
     1441 caacgagcat cgcgatggcg tcgtacgcac aatcgatcga ttcaggtgcg tgcgtcgagc
     1501 gtgaggagcc gatcgaacgg gtcgcggtgg atcatcggca actgcccgat tgcccgtgcg
     1561 agcgcggccg tcaaggcgag ccctgtggcg ccgagcgtac gggggcgttt ggctgactcg
     1621 cgaagaccga ccgccaggaa gtcattgacc accgctccga tgctgcgccg ctcctcgaac
     1681 gcccgctgtc ggacggcttc gtagagatca tcgcgaaggg tcagcgtggt gcgcgcgtgg
     1741 acatcatcgc atcatcgcat cgccctccta tgactcgcag tcgacggaat gccatcaggg
     1801 ctacgaccgg cgctgaaccc cgggcccggc ccgacacgcg agactgaagc catggcactg
     1861 tcgatcgtca ccgccaacgt caacggcatc cgcgccgcgc tcaagcgcga catggagtca
     1921 tgggtcagcg ccgcggcccc cgacatcatg gcgctgcagg aggtccgggc ccccgacgcg
     1981 ctggttcgcg atgtcataga agaactgtgt gagggcgggt ggcacgtcgt gcacagcgag
     2041 gccgccgcga agggacgctc cggtgtcgcc gtcgtgtcgc gggtacccat tgtcggaacc
     2101 atcgacgccg aagtcgcagg attctcggca cggt
//
LOCUS       DAOOMA010000510         2133 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_64945_length_2133_cov_3.508662, whole genome shotgun sequence.
ACCESSION   DAOOMA010000510 DAOOMA010000000
VERSION     DAOOMA010000510.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 2133)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2133)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2133
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64945_length_2133_cov_3.508662"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            415..>2133
                     /locus_tag="PLP95_11865"
     CDS             415..>2133
                     /locus_tag="PLP95_11865"
                     /inference="COORDINATES: protein motif:HMM:NF014026.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 C-terminal
                     domain-containing protein"
                     /protein_id="HPB46542.1"
                     /translation="MSAAEIVAQLDRDTKISLVSGSGFWWTEALPQFGLEQIMVTDGP
                     HGLRQQGAVGDHLGLAGSTPSTCFPPAVTVGSAWDVDLAHDIGVAIGEEANEQGVAVV
                     LGPGLNLKRHPGGGRCFEYYSEDPLLGGKLAAAFVRGTESQGVGTSVKHFAVNNQETY
                     RLVVDAVVDERTLRELYLSGFEIAVTEGRPSTVMSSYNRVNGTYVSEHHELLTSILRE
                     EWGFDGLIMSDWGGTNDRVAGIAAGEDLEMPGSKGPFDPEIRAALDSGALDEAALDAC
                     ATRVVELIQRFQRPASGPSSDRDAHHQLARRAAAAGSVLLSNDGVLPLTPTGTIAVIG
                     SFATSPRYQGAGSSQVSPTRLDNALDALRERVGTDAIVRVAEGFDAKTGNASPAQLDE
                     AVAVATDADVAVVFAGLPARFESEGFDRTTLDLPSGVVALIEAVAATGTPVVVVLNNG
                     GVVHLPWADRVNAVLECWLGGQAGGPAMVDVLFGDAEPGGRLAETIPVHQAQLPVDRF
                     FPGEPRQVQYREGLFVGYRFHDTAGVEPRFAFGHGLSYTSFEWGTPKVTGSGTDLAVS
                     VTVTNAG"
BASE COUNT          333 a          732 c          734 g          334 t
ORIGIN      
        1 ccgctacgac cgcatgaccg ccacccccaa cacccgcatc caccgacccc acgacgcccg
       61 caggtcaaac acaggccgaa cccgcgcaac ccgagggacc ggcggcggaa gggccggacg
      121 acgccccacc gccgggcaac tctcactcac cccgaccaaa ccaccccaac caccgatacg
      181 ccaatgaacc ggcgagcgac ggggcacgcc gggatggatc cgaaacgtgt gcgtcgagtg
      241 tggggctgcc gacggcatgt cgtcaggggc gccagtagga tccgctacat gaccggaccc
      301 agtgcccctc agccgagttc tccccagtcc agtgcccctc agcccacacc cgcttcctct
      361 cctgcgaccg gaacggtcgg ggctgattcg cgcgcttctt cgggtgcgac cgaattgagc
      421 gcggccgaga tcgttgcgca actcgatcgc gacaccaaga tctcgctggt gtcgggcagt
      481 gggttctggt ggaccgaggc gctgccgcag ttcggcctcg aacagatcat ggtgaccgat
      541 gggccacatg ggctgcgcca gcagggcgcg gtcggcgacc atctcggcct ggcgggttcg
      601 accccgtcca cgtgctttcc gccagcggtc acggtcggca gcgcctggga cgtcgacctc
      661 gcgcacgaca tcggcgtggc gatcggcgag gaggccaacg agcagggggt tgcggtcgtg
      721 ctcggcccgg gcctcaacct gaaacgccac cccggtggcg gtcgctgttt cgagtactac
      781 tccgaagacc cgctgctcgg cggcaagctg gcggcggcgt tcgtgcgtgg caccgagtcg
      841 cagggggtgg gcacctcggt gaagcacttc gcggtcaaca accaggagac ctaccggctc
      901 gtcgtcgatg cggtggtcga cgagcgaacg ctgcgcgagc tgtatctgag cgggttcgag
      961 atcgccgtca ccgaggggcg tccgtcgacg gtgatgagtt cctacaaccg ggtgaacgga
     1021 acctacgttt cggaacacca cgagttgctc acgtcgatcc tgcgcgagga gtggggcttt
     1081 gacgggctca tcatgagcga ctggggcggc accaacgacc gtgtggccgg catcgcggcg
     1141 ggtgaggacc ttgagatgcc gggcagcaag ggccccttcg accccgagat ccgcgccgcg
     1201 ctcgactccg gagcgctcga cgaggccgcc ctcgacgcgt gcgccactcg ggtcgtggaa
     1261 ctcatccagc gcttccagcg tcctgcgtcc gggccgagtt cggaccgcga cgcacaccat
     1321 cagctcgctc gtcgcgccgc cgctgcgggc tcggtcctgt tgtccaatga cggagtcctg
     1381 ccgctgacgc cgacggggac gattgcggtg atcgggtcat tcgccacctc gccgcgctat
     1441 caaggcgcgg gcagctcgca ggtgagcccc accaggctcg acaatgcgct tgacgcgctg
     1501 cgcgaacggg tcggcaccga tgcgatcgtt cgggtggctg aggggttcga cgccaagacc
     1561 ggcaacgcgt cgccggcaca actcgacgag gcggtggccg tcgccaccga tgctgatgtc
     1621 gccgtggtgt tcgctgggct gccggcccgc ttcgagtccg agggattcga tcgcacgacc
     1681 ctcgatcttc ccagcggcgt cgtggccctc atcgaggccg tagcggcgac gggcaccccg
     1741 gtggtcgtgg tgctcaacaa cggtggtgtg gtgcacctgc cgtgggccga ccgggtgaat
     1801 gccgtgctcg agtgctggct cggtggccag gccggagggc cggcgatggt cgatgtgctc
     1861 ttcggtgacg ccgagcccgg tgggcggctg gccgagacga ttccggtaca ccaggcgcag
     1921 ctgcccgtcg accgcttctt tccgggtgaa ccccgccagg tgcagtaccg cgaagggctc
     1981 ttcgtcggct accggttcca cgacaccgct ggggtcgagc cgaggttcgc cttcggccac
     2041 ggtctctcct acacgtcctt cgaatggggc actccgaagg tcaccgggtc gggtaccgat
     2101 ctggcggtgt cggtgacggt gaccaacgcc ggc
//
LOCUS       DAOOMA010000511         2126 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_65257_length_2126_cov_3.590536, whole genome shotgun sequence.
ACCESSION   DAOOMA010000511 DAOOMA010000000
VERSION     DAOOMA010000511.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 2126)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2126)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2126
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65257_length_2126_cov_3.590536"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..352
                     /locus_tag="PLP95_11870"
     CDS             <1..352
                     /locus_tag="PLP95_11870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012230794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HPB46543.1"
                     /translation="GAELSDAEIFERQQFAFEAAVRMRDRFLQQEVYERMGLPVKEAV
                     TAMINTPARQMFNLMLFSKIVPNCRKLGLLDRNDQWLRRRFEELGVIQFEDWTDTTDE
                     MEAFALSNEELRIA"
     gene            837..1451
                     /locus_tag="PLP95_11875"
     CDS             837..1451
                     /locus_tag="PLP95_11875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="excalibur calcium-binding domain-containing
                     protein"
                     /protein_id="HPB46544.1"
                     /translation="MIAILLALIVVGVVVGIVVASGGDDAADSDKSDGAVVETTVATV
                     AETTPESITETVPEPTVAESVVPETTRANRDPALLPAGLYCRDLAAQGYSFSAAVTYW
                     RTEGQPDRMDSDRNGIPCETVYSYDDIAAIFPYASAPVSEPLYMRLPSGLLCRDLRDR
                     GVSVRDALDYFIYEGFPDRMDADGNGVPCETVYSDASRIWWNEY"
     gene            1587..>2126
                     /locus_tag="PLP95_11880"
     CDS             1587..>2126
                     /locus_tag="PLP95_11880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB46545.1"
                     /translation="MSEGVELRLVLHAMRLLGTASSERVAERYADLTGLADPAPADRD
                     ADSVVDRVQHLLISAERSGWVSHRSGYFAGWSLTSEGRSHAELLLRAEIEESDTLLDV
                     DAAYRRFVTINRSFLEVCTDWQVLGAGTGTARVNDHSDAEHDLAVLERLEVHHRQVVA
                     ITVDLSGSLGRFGSYRHRFE"
BASE COUNT          328 a          680 c          711 g          407 t
ORIGIN      
        1 cggcgccgag ctgagcgacg ccgagatctt cgagcgccag cagttcgcct tcgaggctgc
       61 cgtgcgcatg cgcgaccggt tcctgcaaca ggaggtctac gagcggatgg ggctgccggt
      121 gaaggaggcc gtgaccgcga tgatcaacac cccggctcgt cagatgttca acctcatgtt
      181 gttctcgaag atcgtgccga actgccgcaa gctcggccta ctcgaccgca acgatcagtg
      241 gctgcgccgc cgtttcgagg aactcggcgt catccagttt gaggactgga ccgacaccac
      301 cgacgagatg gaggcgttcg cgctgtcgaa cgaggaactg cgtatcgcct gagcgccttg
      361 cccgacgcgg tcttgcccgt cgctgcctcg cccgttgcgg tcttgcccgt tctccgataa
      421 ccagtggtcg tgatcacgac cactggttat cggagaacgg tggttttggg gcgacatttc
      481 tggcgatgtt tggggatcag gaggtatcgg cggcgccggt cgctacgctg aatctccaca
      541 tcgaccggtc ggttgccgcg ggtggccgat cgccaaggaa tcggggggct gccatgagtg
      601 gcggtgacga agaccagccc acggggccat atgatcctgc cgcgacgtct cggttcacgc
      661 cgcccagttc ggtgccgccc gcggcgccta gtcagtccgg gtgggcggcg ccgcctccgc
      721 ctccatcacc gccgcctccg atgactccgc caccggcgcc gatggcgact ccgcaagccg
      781 cagtcccgac ctcggctcct gcacctgcgc cgaattcgaa ccgcacgctc ctcatcgtga
      841 tcgcgattct gctcgcactc atcgtcgtcg gtgtcgtcgt cggcatcgtg gtcgcgagcg
      901 gcggtgacga tgccgctgat tccgacaagt ccgacggggc ggtcgtggag acgacggttg
      961 ccacggtcgc tgagacgaca cccgaatcga tcactgagac ggttccggag ccgactgttg
     1021 ccgagtcggt cgttccggag accacccgcg ccaatcgcga tcccgctctg ctgccagcgg
     1081 gcctctactg ccgcgacctg gcggcccagg ggtattcctt ctcggcggcg gtgacgtatt
     1141 ggcgcaccga gggccagccg gatcggatgg actccgaccg caacggaatc ccgtgcgaga
     1201 ccgtgtactc atacgacgac atcgcggcca ttttcccgta tgcgtcggcg ccggtgagcg
     1261 agccgctgta catgcggttg ccgtcagggc tgttgtgtcg cgaccttcgc gatcgcgggg
     1321 tcagtgtgcg cgacgcgttg gactacttca tctacgaggg tttcccggac cgcatggatg
     1381 ccgacggaaa cggcgttccc tgcgagaccg tgtacagcga cgccagtcgc atctggtgga
     1441 acgagtactg acagtccgca ctcgcctgcc ccagcctgtt ctccgataac cagtggtcgt
     1501 gatcacgacc actggttatc ggagaacagg cgttttgggg ggctggtggg ggcgtttggg
     1561 ggtgggctgg ggcgtagtgt ggccgcgtgt ccgagggtgt tgagcttcga ttggtcctgc
     1621 atgcgatgcg cctgctcggt acggcatcga gcgaacgggt cgccgagcga tacgcagatc
     1681 tgacgggact cgccgacccg gcacccgccg accgcgatgc cgattccgtc gtcgacaggg
     1741 tgcagcatct gctcatcagc gccgagcgca gcgggtgggt gtctcatcgc agcgggtatt
     1801 tcgccgggtg gtcgctcacc tccgagggcc ggtcccatgc cgaactgttg ctccgcgccg
     1861 agatcgagga gtccgacacc ttgctcgacg tcgatgcggc gtatcgtcga ttcgtcacga
     1921 tcaaccggtc gtttctcgag gtgtgcaccg actggcaggt gctcggtgcg ggaaccggga
     1981 ccgcccgcgt gaacgatcac agcgacgccg aacacgacct cgccgtgctc gagcgcctcg
     2041 aggtccatca tcgacaggtc gtggcgatca cggtggatct ttccggatcg ttgggtcgtt
     2101 tcggctcgta tcggcatcgc tttgag
//
LOCUS       DAOOMA010000512         2126 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_65261_length_2126_cov_3.197006, whole genome shotgun sequence.
ACCESSION   DAOOMA010000512 DAOOMA010000000
VERSION     DAOOMA010000512.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 2126)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2126)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2126
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65261_length_2126_cov_3.197006"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1435)
                     /locus_tag="PLP95_11885"
     CDS             complement(<1..1435)
                     /locus_tag="PLP95_11885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012223078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HPB46546.1"
                     /translation="MSASAPNDRVVNPGIRNVAIIAHVDHGKTTLVDAMLRQSGAFRE
                     GAEVADRIMDSMDLEREKGITITAKNTAVRWHSPDGDEVKINIVDTPGHADFGGEVER
                     ALSMVDGVVLLVDSSEGPLPQTRFVLRKALNLELPVVLVINKIDRADARISEVVDEVY
                     ELFLDLDANEDQIDFPIVYCSARAGTATLDLDEPGTDLQPLFTTLVDTVSPPAADVSR
                     PLQALVTNLASDPYVGRLARCWVVNGSIKRNQTLSWCRADGTIERAKIGVIYMTESHE
                     RIEVESADAGEIIEVSGIEEVNIGDTLADLDNPDALPVITVDEPSLSMTIGINTAPLN
                     GQDGSKLTARQIKARLETELVGNVSLRVLPTDRPDAWEVQARGELQLAILIEQMRREG
                     FELTVGKPQVVTKEIDGKVHEPVERLTVDCPEEHMGAVSSLVAPRKARLENMANHGTG
                     WIRLDYLIPARGLIGFRTEFLTETRGTG"
     gene            complement(1544..>2126)
                     /locus_tag="PLP95_11890"
     CDS             complement(1544..>2126)
                     /locus_tag="PLP95_11890"
                     /inference="COORDINATES: protein motif:HMM:NF013927.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HPB46547.1"
                     /translation="DGSTPLWAEAFDPSRPFDTPAGLAPAPADAPDPVITKLTEAVRR
                     LELAGFAADSTLGDVQFALRNGERVGVPGGSWVDGAIAIGSSAQSWTITDPALKSFAE
                     RKDVADDSTLTTGDDQNGYLVNYGNSFVLAVEFTKDGPAAKAFLTYSNTEDRTDPNYL
                     EATTRYAAKDWRDVEFREESVAAATTATYRVRG"
BASE COUNT          329 a          637 c          767 g          393 t
ORIGIN      
        1 tgccggtgcc gcgggtctcg gtgaggaact cggtgcggaa gccgatgagg ccgcgagccg
       61 gaatgaggta gtcgaggcgg atccagccgg tgccgtggtt cgccatgttc tcgagccggg
      121 ccttgcgcgg cgcgaccagg gagctcaccg cgcccatgtg ctcttcgggg caatcgacgg
      181 tgagtcgctc gaccggttcg tggaccttgc cgtcgatttc cttggtgacg acctgcggct
      241 tgccgacggt caattcgaag ccctcgcgac gcatctgttc gatgaggatg gcgagctgca
      301 gttcgccgcg ggcctgcacc tcccaggcgt cggggcggtc ggtcggcagc acgcgcagcg
      361 atacgttgcc gacgagttcg gtttcgagtc gggccttgat ctgacgggcg gtgagcttgg
      421 agccgtcctg tccgttgagc ggcgcggtat tgatgccgat cgtcatcgac agcgacggtt
      481 cgtcgacggt gatgacgggc agcgcgtccg ggttgtcaag gtcggcgagg gtgtcgccga
      541 tgttgacctc ttcgatgccg gagacctcga tgatctctcc ggcatcggcc gattcgacct
      601 cgatgcgctc gtggctctcg gtcatgtaga tgacgccgat cttggcgcgt tcgatcgtgc
      661 cgtccgcccg acaccacgac agggtctgat tgcgcttgat ggaaccattg acgacccagc
      721 agcgtgcgag gcgaccgacg taggggtccg acgcgaggtt ggtgacgagc gcctgaagcg
      781 gtcgcgagac gtcggccgct ggcgggctca ccgtgtcgac cagggtggtg aacagcggtt
      841 gcaggtcggt gccgggttcg tcgaggtcga gggtcgcggt gccggcgcgg gccgagcagt
      901 agacgatcgg gaagtcgatc tggtcttcgt tggcgtcgag gtcgaggaac agctcgtaca
      961 cctcgtcgac gacctcggag atgcgtgcgt cggcccggtc gatcttgttg atcaccaaca
     1021 cgacgggcaa ctcaagattg agcgccttgc gcagtacgaa acgcgtctga ggcagcggac
     1081 cttcggagga gtcgaccaac aggaccactc cgtcgaccat cgaaagggct cgttcgacct
     1141 cgccaccgaa atccgcgtgg ccgggggtgt cgacgatgtt gatcttcacc tcgtcgccat
     1201 cgggggaatg ccagcgcacc gccgtgttct tggcggtgat ggtgatgccc ttttcgcgtt
     1261 cgaggtccat cgaatccatg attcgatcgg cgacttcggc cccctcgcga aaggcgccgg
     1321 actgacgcaa catggcatcg acaagggtgg tcttgccgtg gtcgacgtgg gcgatgatcg
     1381 cgacgttacg gattccgggg ttgacgacgc ggtcgttggg cgcggacgcg ctcatggtga
     1441 ctccaactga attcggggga cccgacagtt tcgcacgcga cggtgccgac caccgagttt
     1501 tgccctcgaa cgccctcgga tgaaggcgtt cgagggcgtc gagtcagccg cgtacccggt
     1561 aggttgcggt cgtcgccgcc gccacggact cctcgcgaaa ctccacgtcg cgccagtcct
     1621 tcgccgcata ccgcgtggtg gcctcgaggt agttcgggtc ggtgcggtcc tcggtgttgg
     1681 agtaggtgag aaacgccttg gcggccggtc cgtccttggt gaactcgacg gccagcacga
     1741 acgagttgcc gtagttgacg agatagccgt tctggtcgtc gccggtggtg agcgtggagt
     1801 cgtcggcgac gtccttgcgc tcagcgaacg atttgagcgc cggatcggtg atggtccacg
     1861 actgcgccga ggagccgatg gcgatggcgc cgtcgaccca cgaaccgccg ggaacgccca
     1921 cccgttcgcc gttgcgcagg gcgaactgca cgtcgcccaa cgtcgagtcg gccgcgaacc
     1981 cggcgagttc cagtcgacga acggcctcgg tcagcttcgt gatgacgggg tcgggggcat
     2041 cggccggcgc cggagccagg ccggcggggg tgtcgaaggg acgcgagggg tcgaatgcct
     2101 cggcccacag cggcgtggaa ccgtcg
//
LOCUS       DAOOMA010000513         2120 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_65535_length_2120_cov_3.635351, whole genome shotgun sequence.
ACCESSION   DAOOMA010000513 DAOOMA010000000
VERSION     DAOOMA010000513.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 2120)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2120)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2120
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65535_length_2120_cov_3.635351"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(10..468)
                     /locus_tag="PLP95_11895"
     CDS             complement(10..468)
                     /locus_tag="PLP95_11895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012228867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HPB46548.1"
                     /translation="MTTEITGIEGLKSLEGKHLGYSDYLEVTQERVNQFAEATGDHQW
                     IHVDPERAKDGPFGGTIAHGYLTLSLGPMLAPQVMKMGGFKMAVNYGCGKVRFPSPVP
                     VGANLRLGIEVTQVEDLGEGAAQCTFLYTFETEGSSKPSCVSEVIIRVYE"
     gene            complement(503..1480)
                     /locus_tag="PLP95_11900"
     CDS             complement(503..1480)
                     /locus_tag="PLP95_11900"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: protein motif:HMM:NF013827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HPB46549.1"
                     /translation="MFRFAPQRYSNDGWFRVGSVDVTTTILVLGVSVLSLFLTAISQA
                     TFFDPYSLNGHQVRSGQVWRIATWPFANFPNIWFILSAFIFFMLGTQLEGDVGRKRFA
                     WMLAILTVVPGIVGVLYPVGWGISGASLHTGAEAFGLSNLTTGLLVIFALRFPFARSL
                     LNIPLWVLLAVFETIYALQIIAERDWAMLVFFLASLAVAALIARAFDLTEFHQIPKLP
                     LPGFVSGDPYAKTNRSRARKNKQQAGSSTSRGNSTGRPTGKGRSPGSPFARSTPAEVI
                     PLRPEPRLSREAQAELDTLLDKISEGGMDSLTVDERARLDELSRTLRGE"
     gene            complement(1485..>2120)
                     /locus_tag="PLP95_11905"
     CDS             complement(1485..>2120)
                     /locus_tag="PLP95_11905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012228860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HPB46550.1"
                     /translation="NIVFMGMGEPLANYDVTWAAVERLHGDVGISARHLTISTVGIVP
                     GIRRLTLESLPVNLAVSLHAANDTLRDELVPINRRYPLEVLAQACAEYLAVKNRRLSF
                     EWAMIDGVNDTERDTRELAAYATSLRAHVNLIPLNPTPGYPTVGSPMRRVREFRDRLD
                     SLGANATIRQNRGTEIDAACGQLRNVHRSRGTAKVATPVSLAQDRGVSLPE"
BASE COUNT          371 a          624 c          753 g          372 t
ORIGIN      
        1 ctgtgttgcc tactcgtaga cgcggatgat gacctcggag acgcaactcg gcttcgacga
       61 gccctcggtc tcgaaggtgt acaggaaggt gcactgcgcg gcgccttcgc cgaggtcctc
      121 gacctgggtg acctcgatgc cgaggcggag gttggcgccg accggaaccg ggctcgggaa
      181 gcgaaccttg ccacagccgt agttgacggc catcttgaag ccgcccatct tcatgacctg
      241 aggtgcgagc atcgggccga gcgacaaggt gaggtagccg tgtgcgatcg tgccgccgaa
      301 ggggccgtcc ttggcgcgtt cgggatcgac gtggatccac tgatgatcgc ccgtcgcctc
      361 ggcgaactgg ttgacgcgtt cctgggtgac ctcgaggtag tcgctgtatc cgaggtgctt
      421 tccttcgagg gacttcaagc cctcaatgcc ggtgatctcc gttgtcatgg cgcagattct
      481 tacgcacccg cacccaaggc cgctactcgc cgcggagggt ccggctgagc tcgtcgagac
      541 gagcgcgctc atcgacggtg aggctgtcca tgccgccttc gctgatcttg tcgagcagcg
      601 tgtcgagttc ggcttgtgcc tcgcggctca ggcgcggctc gggcctcagc ggaatgacct
      661 ctgcgggggt ggatctggcg aacggtgaac ccggcgaacg gcccttgccg gtcggcctgc
      721 cggttgagtt gcctctggac gtggacgagc cggcctgctg tttgttcttg cgggcccggg
      781 atcggttggt tttggcgtac gggtcgccgc tgacgaaccc cggcagcggc agtttgggga
      841 tctggtgaaa ctcggtgaga tcgaaggccc gggcgatgag cgccgcgacg gcgagcgagg
      901 cgaggaagaa gaccagcatc gcccagtcgc gttccgcgat gatctgcagg gcgtagatcg
      961 tctcgaacac ggccaacagc acccacaacg ggatgttgag caaggaccgg gcgaagggga
     1021 aacgcagcgc gaagatcacc aggaggccgg tcgtcagatt cgacaggccg aacgcctccg
     1081 ctccggtgtg gagcgacgct ccggagattc cccaacccac cgggtacagc acgccgacga
     1141 tgcccggaac gacggtgagg atcgccagca tccatgcgaa gcgcttacgg ccgacgtcgc
     1201 cctcaagttg ggtgccgagc atgaagaaga tgaacgccga gaggatgaac cagatgttgg
     1261 ggaagttcgc gaacggccac gtcgcgatcc tccagacctg acccgatcgg acctgatgtc
     1321 cgttgagcga gtaggggtcg aagaacgtcg cctggctgat cgcggtgagg aacagtgaca
     1381 ggaccgacac gccgaggacc aagatggtgg tggtgacgtc gacggagccg actcgaaacc
     1441 agccgtcgtt gctgtagcgc tgaggagcga agcgaaacac gaggtcattc tggcagcgaa
     1501 acgccgcgat cttgtgccag cgacaccggt gtggcgacct tggcggtgcc gcggctgcga
     1561 tgaacgttgc gaagttgccc gcaggctgcg tcgatttcgg tgccgcggtt ctggcggatg
     1621 gtggcgttcg ctccgaggga atcgagtcgg tcccggaact cgcggacgcg ccgcatgggc
     1681 gagccgacgg tggggtatcc gggggtcggg ttgagcggga tgaggttgac gtgtgctcga
     1741 agcgacgtgg cgtaggccgc cagttcccgg gtgtcgcgtt cggtgtcgtt gaccccgtcg
     1801 atcatcgccc actcgaagct caaccgtcgg ttcttgactg ccaggtactc ggcgcacgcc
     1861 tgcgccaaca cctccagcgg gtaacgcctg ttgatgggca cgagttcgtc tcgaagcgtg
     1921 tcgttcgcgg cgtgaaggga aaccgccagg ttcaccggta gcgactcgag ggtgagtcgt
     1981 cggatgccgg ggacgatgcc gacggtcgag atcgtgaggt gacgggccga gatgccgacg
     2041 tcgccgtgga gccgctcgac cgccgcccag gtcacgtcat agttggcaag cggctcgccc
     2101 atgcccatga acacgatgtt
//
LOCUS       DAOOMA010000514         2113 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_65870_length_2113_cov_3.212828, whole genome shotgun sequence.
ACCESSION   DAOOMA010000514 DAOOMA010000000
VERSION     DAOOMA010000514.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 2113)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2113)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2113
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65870_length_2113_cov_3.212828"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..498)
                     /locus_tag="PLP95_11910"
     CDS             complement(<1..498)
                     /locus_tag="PLP95_11910"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF013015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HPB46551.1"
                     /translation="MNTTTGRDGPERVILHVDMDAFFASVEILDDPSLRGRPVVVGAS
                     GPRGVVAAASYEARAYGVHSAMPGALARRRCPQAVFLAGRHGRYREVSAEVMAIFGEV
                     TPLVEPLSLDEAFLDVTGRRRALGDGPRIAAAIRQRVLDEVGLTCSIGVAPNKFLAKL
                     ATETAK"
     gene            complement(509..1003)
                     /locus_tag="PLP95_11915"
     CDS             complement(509..1003)
                     /locus_tag="PLP95_11915"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HPB46552.1"
                     /translation="MREWVVAGAVIENDAGLLLVENLRRNGATDWTPPGGVIEADDAE
                     DIRSGLAREVHEETGLTVAGWGELLYEVVAEAPALGWVMRAQIHLVTAVIGEVVVGND
                     PDGIVTDAAYVPVDACAAQLTSTHRWVSEPLTDWLGQRWSEPRSYRYRLEPHRSGPGR
                     IVRL"
     gene            complement(1000..>2113)
                     /locus_tag="PLP95_11920"
     CDS             complement(1000..>2113)
                     /locus_tag="PLP95_11920"
                     /inference="COORDINATES: protein motif:HMM:NF012363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="carboxylesterase family protein"
                     /protein_id="HPB46553.1"
                     /translation="ALAWVRDNIAAFGGDPNRVTLFGESAGAMSAAVHLASPGSRGLF
                     RRVIAQSGAAGHVQSAASGTKVAEAVLDALGLGPGPFGSGAGRGGHRLLDRLQGVSAA
                     ALRQAVHTVSRQSVGDTALAFCPTIDGVTISEHPVESLRSGSSCDVDLLCGTNADEMN
                     LFRVQDLLSGTSEELNEDRLRRRLARALEFWGSSVGLDEALARYRDRYPYQDNSQLWS
                     TISTDTTFRAHMFATADAHAARGRVWSYHFTQPSSAFGGALGACHAVEVPFVFDNLDQ
                     PGVEVLLGELTPRRRRFAAALADTWAQFASVDDPSQIRVPATSDCWLRYRTGPRYQAV
                     LDLESRQVEGLDADLMSIWVRTPEARSGVESGKMVS"
BASE COUNT          362 a          761 c          690 g          300 t
ORIGIN      
        1 cttggcggtc tcggtggcga gcttggcgag gaacttgttc ggcgcaaccc cgatcgaaca
       61 ggtcaatccg acctcgtcga ggacgcgctg tcgaatggcc gcagcgatgc gaggtccatc
      121 gccgagggca cgccgccggc cggtgacgtc gagaaacgcc tcgtcgagcg acagcggttc
      181 gaccagcggt gtcacctcgc cgaagatggc catgacctca gcactgacct cgcggtagcg
      241 accgtgacga ccggccagga acaccgcctg ggggcatcga cggcgcgcca gcgcacccgg
      301 catcgccgaa tgcacgccat aggcgcgggc ctcataggag gcagccgcga cgacccctcg
      361 gggacccgac gcccccacca ccaccggacg accccgcaac gacgggtcat cgagaatctc
      421 caccgacgcg aagaacgcat ccatgtcgac gtgcagaatc acccgctcgg gcccgtcgcg
      481 gccggtggtc gtgttcacca cgcctcgctc acaggcgcac gatgcgcccc ggcccgctgc
      541 ggtgaggttc gagccggtaa cggtaactgc gcggttcgga ccagcgctgc ccgagccagt
      601 cggtcagcgg ttcgctcacc caacgatggg tgctggtgag ctgtgcagca cacgcgtcga
      661 ccggcacgta ggccgcgtcg gtcacgatgc cgtcggggtc gttgccgacc acgacctcgc
      721 cgatgaccgc ggtcaccaga tggatctgcg cacgcatcac ccacccgagc gccggagcct
      781 cggccaccac ctcatagagc aactcgcccc atccggcgac ggtcagtccg gtctcctcgt
      841 gcacctcgcg cgcgagtccg ctgcggatgt cctcggcgtc gtcggcctcg atgaccccgc
      901 cgggtggggt ccaatcggtc gcaccgttgc gccgcaggtt ctccacgagc aacagcccgg
      961 catcgttttc gatgaccgct ccggcgacga cccactctct cacgacacca tctttcccga
     1021 ttcgacgccg cttcgcgcct cgggtgttcg gacccagatc gacatcaggt cggcgtcgag
     1081 gccttcgacc tgacgcgatt cgaggtccag gacggcctgg tatcgcggcc ccgtgcgata
     1141 gcgcagccag caatccgagg tggccggaac ccggatctgc gacgggtcgt cgaccgaggc
     1201 gaactgcgcc cacgtgtcgg cgagagccgc ggcgaaacga cggcgtcgtg gggtcaactc
     1261 gccgagcaga acctcaaccc cgggctgatc gaggttgtcg aacacgaacg gaacctccac
     1321 cgcgtggcag gcgccgagcg caccgccgaa ggcgctcgac ggttgggtga agtgatacga
     1381 ccacacccgg ccccgtgctg cgtgggcgtc ggccgtggcg aacatgtgcg cccggaaggt
     1441 cgtgtcggtg ctgatggtgc tccacagttg cgagttgtcc tgatacgggt accggtcgcg
     1501 atagcgggcg agcgcctcgt cgaggccgac ggacgaaccc cagaactcga gcgcccgtgc
     1561 cagacgccgc cgcaatcgat cctcgttcaa ctcctccgat gtccccgaca gcaggtcctg
     1621 cacccgaaac aggttcatct cgtccgcgtt ggtgccacac agcaggtcga cgtcgcagga
     1681 actcccgctc cgcaacgact cgacggggtg ctcggagatc gtgaccccgt cgatcgtcgg
     1741 acagaacgcc agcgcagtgt cgccgacgga ctgacgtgac acggtgtgta ccgcctggcg
     1801 aagcgctgcg gccgaaaccc cttggaggcg atcgaggagt cggtgaccgc cccggcctgc
     1861 accgctgccg aagggacccg ggccgagccc gagcgcatcg agaaccgcct cggccacctt
     1921 ggttcccgag gccgccgact gcacgtggcc tgcggcgccg gactgcgcga tgacccgccg
     1981 aaacagtcca cgggacccgg gtgaggccag gtgcacggcg gcgctcatcg cccccgcact
     2041 ctcgccgaac agcgtcaccc gatttgggtc tccgccgaag gcagcgatgt tgtcgcgaac
     2101 ccaggccaac gcg
//
LOCUS       DAOOMA010000515         2109 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_66032_length_2109_cov_4.618793, whole genome shotgun sequence.
ACCESSION   DAOOMA010000515 DAOOMA010000000
VERSION     DAOOMA010000515.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 2109)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2109)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2109
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66032_length_2109_cov_4.618793"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..398
                     /locus_tag="PLP95_11925"
     CDS             <1..398
                     /locus_tag="PLP95_11925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB46554.1"
                     /translation="VSSDPQINAVARARLAHPVELPVGGLLVMAVLIGSFAKILLSST
                     KTEAIIVASIIAALIFAVAVIIGTRPQMRRTAVVTSVIVGFLVVIVLGLIGAIRGDSP
                     VEHHDSGDHASAVATDYRLASALGETEAS"
     gene            407..1603
                     /gene="coxB"
                     /locus_tag="PLP95_11930"
     CDS             407..1603
                     /gene="coxB"
                     /locus_tag="PLP95_11930"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR02866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit II"
                     /protein_id="HPB46555.1"
                     /translation="MTKPHRLSRLVSVSAITSAALFLSSCGYVRDSENHPLTTLDPKG
                     PQAQMIDDLSTPVFAVAGVVFVLIQALMIYLIVRYRRRPDDVDGVDEPTQIHGMDRLE
                     WAWTITPAVILAGLAILNVQTIWKMEERNDPNMITVEIIGQQWWWEYRYDFDADGSVD
                     VITANQMVIPEDTLVDLHIRSNDVIHSFWIPALNGKRDAVPGRTNRWLLQADDPGLYE
                     GTCTEFCGLSHAYMRMEVKALDSADWEQWKSDQLEPANIPADPTPDDDSDDDAVLTGY
                     KTFIANCASCHQLNGVDAGGEETDGTPDADYRGEDHPLTAGNAPNLTHLMSRNRYAGN
                     LFSLWTTDSDGNYTDQVDTSTLGDWLRDPGALKPMAADANRGMPNLQLSEDQISSLVA
                     FLSTLK"
     gene            1613..>2109
                     /locus_tag="PLP95_11935"
     CDS             1613..>2109
                     /locus_tag="PLP95_11935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020379246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HPB46556.1"
                     /translation="MAIIEAPAPVALESGQPAARPLGIFARPTSAQTGWKSWLTSVDH
                     KRIGIMYGMASMFFFVIGGSEALLIRLQLARPNQALLSADLYNQVYTMHGVTMVFLVV
                     MPLAAAFANYLLPLQIGARDVSFPRMNALSFWLYFLGGLFLNSSWFLGGGADGGWFNY
                     APNSGV"
BASE COUNT          380 a          675 c          641 g          413 t
ORIGIN      
        1 gggtgtcatc ggatccgcag atcaacgccg tggcccgtgc ccggctcgcg caccccgtcg
       61 aattgcccgt cgggggcctg ctggtcatgg cggtcctgat cggatccttc gcgaagattc
      121 tgctctcgtc gaccaagacc gaagcgatca tcgtggcatc gatcatcgcg gcgctgatct
      181 tcgcggtagc ggtgatcatc ggaactcgcc cgcagatgcg ccgtactgcg gtggtcacct
      241 cggtcatcgt cggatttctc gtggtgattg tgttgggtct gatcggagcg atccgcggcg
      301 actcgccggt tgagcaccac gacagcggcg accatgcatc ggccgtcgcg acggattacc
      361 ggttggcctc tgccttggga gagactgaag catcatgatt gagcgaatga cgaagcccca
      421 ccgactttcg aggttggtgt cggtcagcgc tatcacctcc gcggcgttgt tcctgtcgag
      481 ctgcggatac gtgcgcgaca gcgagaatca cccgctcacc acgctcgacc ccaaggggcc
      541 tcaggcgcag atgatcgacg atctctcgac gccggtcttc gcggtggcgg gggtcgtgtt
      601 tgtcctgatc caggcactca tgatctacct gatcgtgcgc taccgccgtc gccccgacga
      661 tgtcgacggc gtcgacgagc ccactcagat ccacggcatg gaccggctcg aatgggcgtg
      721 gaccatcact cccgcggtca tcctcgccgg cctcgcgatt ctcaacgtcc agacgatctg
      781 gaagatggaa gaacgcaacg atccgaacat gatcacggtg gaaatcatcg gtcagcagtg
      841 gtggtgggag taccgctacg acttcgatgc cgatgggtcc gtcgacgtca tcaccgccaa
      901 ccagatggtg attcccgagg acacgctggt cgatttgcac atccggtcca acgacgtgat
      961 ccactcgttc tggatcccgg cgctcaacgg aaagcgcgac gccgttcctg gccgcacgaa
     1021 ccgctggctg ttgcaggccg acgaccccgg cctctacgag gggacctgca ccgagttctg
     1081 cggtctgagc cacgcgtata tgcgcatgga ggtcaaggca ctcgattcag cggattggga
     1141 gcagtggaaa tccgaccagc tcgaacccgc gaacatccct gccgatccca cgccggatga
     1201 cgattccgac gatgacgcgg tgctcaccgg ctacaagacg ttcatcgcca actgtgcgtc
     1261 gtgtcatcag ctcaacggcg tcgacgcagg cggcgaggag accgacggca ccccggatgc
     1321 ggactatcgc ggtgaggatc atccactgac ggcgggcaat gccccgaacc tcacccacct
     1381 gatgagccgt aatcgctatg cgggcaacct gttttcgctg tggaccaccg atagcgacgg
     1441 caactacacc gaccaggtcg acacgtcgac cctcggcgac tggttgcgcg atcccggggc
     1501 gctgaagccg atggcagccg acgcgaaccg cggcatgccg aacctgcaac tgtccgaaga
     1561 tcagatctcg tcgctggtcg cgttcctgtc gacgctcaag taggagaccg atatggcaat
     1621 cattgaagct ccggcgccgg tggctctcga atcgggtcag ccagccgcac gccccctggg
     1681 cattttcgcc cgaccgacct ccgcgcaaac cggttggaag tcgtggctca cgtcggtcga
     1741 tcacaagcgc atcggcatca tgtacggcat ggcctcgatg ttcttctttg tcatcggtgg
     1801 cagcgaagcc ttgttgatcc ggcttcaact cgcccgaccg aaccaggcgt tgttgagtgc
     1861 tgacctgtac aaccaggtgt acacgatgca tggcgtcacg atggtgttcc tcgtggtcat
     1921 gccactcgcc gcagcgtttg cgaactacct cctgccactg cagatcggcg cccgggatgt
     1981 gtccttcccg cgaatgaacg cgctgtcgtt ctggctctac ttccttggtg gcctgttcct
     2041 gaattcgtcg tggttcctcg gcggaggcgc cgacggtggc tggttcaact acgcaccgaa
     2101 ctcgggtgt
//
LOCUS       DAOOMA010000516         2109 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_66033_length_2109_cov_4.557449, whole genome shotgun sequence.
ACCESSION   DAOOMA010000516 DAOOMA010000000
VERSION     DAOOMA010000516.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 2109)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2109)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2109
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66033_length_2109_cov_4.557449"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            169..1089
                     /locus_tag="PLP95_11940"
     CDS             169..1089
                     /locus_tag="PLP95_11940"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021623.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HPB46557.1"
                     /translation="MTILDPPIATEITSAGPEVGSTNPSAHRQIPVRHLHTDPTAVES
                     GWLVPDDPIFSHLLATLSAVFPNGEDFFVAAVRRHRDAVGDNPTLKAQVKGFIGQEAM
                     HGRAHRQLNARLAELGYHTVRADHLIEVIVARILQAPPRTLAIAATAAAEHYTGLLAE
                     SVLGHEPTRKILFGHSAVEPLICWHALEELEHKNVAFDVMDAAGAGYPTRALGFGVTT
                     TVLAGYVAAEWARAVFEDRHRITRAHRRRFRSNLARQRLLSWWFLRRAFMYLRPGFHP
                     DDTGTDQLVETWRYRLSQIATVPETKAARS"
     gene            1086..1343
                     /locus_tag="PLP95_11945"
     CDS             1086..1343
                     /locus_tag="PLP95_11945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB46558.1"
                     /translation="MSAADPPTTCAPAGIDALDDPIFQLDGWAQHGAPFDWFAARRLE
                     SPVYAHARNPHAGAGAPQVWSVLVHTNVVQLGRCPDVLSSD"
     gene            1371..>2109
                     /locus_tag="PLP95_11950"
     CDS             1371..>2109
                     /locus_tag="PLP95_11950"
                     /inference="COORDINATES: protein motif:HMM:NF012296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HPB46559.1"
                     /translation="MGHELQAVNDRTIDSVGVSREMVVAASKHLLTLDPPEHTSYRKI
                     VNRGFTPRQIGLLESTVRELLNDLLDQHTTREAFDFTTEVSMPLPVNIIARMIGSPAE
                     SDALLLRWSNEAIASTDLEYSAGEMSQLGAVMSLIGHFNTLRVEREAVPSDDLISLLL
                     DAEVDGESLTDTRLMLFLILLTAAGNETSRTAFSHAVVEFANHPDEWQRLREHPELIR
                     SAVEEIVRYSSPVLYFRRNAVEDLDIAG"
BASE COUNT          363 a          789 c          622 g          335 t
ORIGIN      
        1 cgacgaagtc atccggcggg ccacctccat gatcgacggc gccctagcgg cggtcaccgc
       61 cccaaccccg gagccgacag cgccgtcgga agcgctgtcg accaccgtcg ccacgacacc
      121 acaccgcgat acccaaccca cccgcacgaa cctctcggag gaccggccgt gaccatcctt
      181 gacccaccca tcgcaaccga gatcacgtcc gccggacccg aggtcggatc cacgaatccg
      241 tcggctcacc gccagattcc tgtgcggcat ctccacaccg accccaccgc cgtcgagtcg
      301 ggctggctcg tccccgacga cccgatcttc agccacctcc tcgccacgct gtcggcggtg
      361 tttcccaacg gcgaggactt cttcgtcgcc gccgtgcgtc ggcaccgcga cgcagtcggc
      421 gataatccga cgctcaaggc gcaggtcaag gggttcatcg ggcaggaggc catgcacggc
      481 cgagcgcacc gacagctcaa cgccaggctc gccgaactcg gataccacac ggttcgcgcc
      541 gatcacctca tcgaggtcat cgtcgcccgg atccttcagg caccgccccg gacgctcgcc
      601 atcgcggcga ccgccgccgc cgagcattac acgggcctgc tcgccgagtc ggtgctcggc
      661 cacgagccga cccgcaagat cctgttcgga cattccgcgg tcgagccgct gatctgttgg
      721 catgcgctcg aggagctcga acacaagaac gtcgccttcg acgtcatgga cgccgcgggc
      781 gccggatacc ccacccgggc gctcggcttc ggcgtcacca ccaccgtgct ggccggctac
      841 gtcgcggccg agtgggcccg ggcggtgttc gaggatcgtc accgcatcac ccgagcacac
      901 cggcgccggt tccgatccaa cctcgcccgc caacgcctcc tcagctggtg gttcctgcgg
      961 cgggcgttca tgtacctgcg accgggcttt caccccgacg acaccggaac cgaccaactc
     1021 gtcgagacgt ggcgatatcg cctctcgcag atcgccacgg ttcccgaaac gaaggcggcc
     1081 cgttcgtgag tgccgccgac ccgcccacga cctgcgcgcc cgcgggcatc gacgcgctcg
     1141 acgacccgat ttttcagctc gatgggtggg cgcaacacgg agccccattc gattggttcg
     1201 ccgcccgtcg tctcgaatcg cccgtctacg cccatgcgcg caatccgcac gcgggggccg
     1261 gcgcgccgca ggtgtggtcg gtgctcgtcc acaccaacgt cgtgcaactc ggtcgctgcc
     1321 cggacgttct ctcctccgac taggaacgcg gcggcgcgac cgggctcggg gtggggcacg
     1381 aacttcaagc ggtcaatgac cgaacgatcg acagcgtcgg cgtctcacgg gagatggtcg
     1441 tggcggcatc gaagcatctc ttgaccttgg acccacccga gcacacctcc tatcgcaaga
     1501 tcgtgaaccg agggttcaca ccacgccaga tcggccttct cgagtcgacg gtgcgcgaac
     1561 tcctcaacga cctgctcgat cagcacacca cccgcgaggc cttcgatttc accaccgagg
     1621 tttcgatgcc gctgccggtc aacatcatcg cccggatgat cggttcgccc gcagaatctg
     1681 acgccctgct cctgcgatgg agcaatgagg ccatcgcgtc gaccgacctc gagtactcgg
     1741 ccggcgagat gtcccaactc ggagcggtca tgtcgctcat cggccacttc aacacgttgc
     1801 gcgtcgaacg cgaggcggtc ccgagcgacg atctcatctc gttgctgctc gatgcggagg
     1861 tcgacgggga gtcgctcacc gatacccgcc tcatgttgtt cctcatcttg ttgaccgccg
     1921 ccggaaacga gaccagcaga accgcgttca gccatgcggt cgtcgaattc gcgaatcacc
     1981 ccgacgagtg gcagcggctg cgcgaacacc ccgagctcat ccgctcggcg gtcgaggaga
     2041 tcgtgcgata ttcgtcaccg gtgctgtact tccgccgcaa tgcggtcgaa gacctcgata
     2101 tcgccgggc
//
LOCUS       DAOOMA010000517         2102 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_66330_length_2102_cov_4.539326, whole genome shotgun sequence.
ACCESSION   DAOOMA010000517 DAOOMA010000000
VERSION     DAOOMA010000517.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 2102)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2102)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2102
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66330_length_2102_cov_4.539326"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..691
                     /locus_tag="PLP95_11955"
     CDS             <1..691
                     /locus_tag="PLP95_11955"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HPB46560.1"
                     /translation="AANETADMVKTIGPAASVHVVDVSDVAQLEALPNEVLEAHGGVN
                     ILINNAGVTSAGSFERESVEDLQWIVDINMWGVVHGCRVFLPHLHEADEAHIVNLSSM
                     VGLVGLPHNVAYSLTKGAVRGFTEGLRAELIRTSIGVTTVFPGSINTNITQHARGAER
                     DRLARMGKNRLAPIVMRPPAAVANGIVKGIERNKARVVVGPDAHMVSLITRFAPGRSG
                     LIGRLTSRVAE"
     gene            827..1345
                     /locus_tag="PLP95_11960"
     CDS             827..1345
                     /locus_tag="PLP95_11960"
                     /inference="COORDINATES: protein motif:HMM:NF022072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HPB46561.1"
                     /translation="MGKGEIVECERVDLGFLSDAPIKVTVNQVVACTGQRLWDILADG
                     ESWTRWVKPIVGVEWTSPKPLGIGTTRTVTMVGGLVGYEEYLAWDAPRHFAFRFNQTT
                     KGGPEAFVEEYRIYDLGNGSCKVSWTMAMRIGGPSARMPGVVAAGMKKANASFLKKLA
                     SYAASNPVLADD"
     gene            complement(1346..>2102)
                     /locus_tag="PLP95_11965"
     CDS             complement(1346..>2102)
                     /locus_tag="PLP95_11965"
                     /inference="COORDINATES: protein motif:HMM:NF012296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HPB46562.1"
                     /translation="AAGPDDVEALMAGAEAFEGYTSHIAGLIEARRADPGEDLISILT
                     AAYDAGELKYEEGHLQNYEGGETVSDLSSDELLMFCVLLMVAGNETTRNAIAGGMRAF
                     SQNPDQRDLLIANPDLIDSAVEEIVRFTSPVMNFTRTVAKPITLHGVDLVEGDVVFML
                     YQSANRDNRVFDNPEEFRIDRNPNPHVAFGFGTHYCMASNLARMEIKVVFEELFRRLP
                     DITLSDPHGPIDRSPSAFVAGIHSLPATFTPVG"
BASE COUNT          374 a          658 c          704 g          366 t
ORIGIN      
        1 cgcagccaac gagaccgccg acatggtcaa gaccatcggg ccggccgcct cggtgcacgt
       61 cgtcgacgtg agtgacgtcg cgcagcttga ggcgttgccg aatgaggtcc tcgaggcgca
      121 cggtggcgtg aacatcctca tcaacaacgc cggggtgact tccgctggtt ccttcgaacg
      181 ggagtcggtc gaggacctgc agtggatcgt cgacatcaac atgtggggag tcgtgcacgg
      241 gtgtcgcgtt ttcctgccgc acctgcacga ggcggacgag gcccacatcg tgaacctttc
      301 gtcgatggtc ggactcgtcg ggctgccaca caacgtcgcg tactcactga ccaagggagc
      361 ggtgcgcggg ttcaccgagg ggcttcgagc ggagctcatc cgtaccagca tcggtgtcac
      421 cacggtgttt ccggggtcga tcaacaccaa catcacccaa cacgcgcggg gcgcggaacg
      481 agatcggctc gctcgcatgg gtaagaaccg gctcgcaccc atcgtgatgc gtccacccgc
      541 agccgtggcg aacggcatcg tgaaaggcat cgagcgcaac aaggcccgtg tcgtcgtcgg
      601 tcccgacgct cacatggtca gcctgatcac ccgcttcgcg cccggtcgct cgggactcat
      661 cggacggctc accagccgcg tcgccgagta gccagcgctg accggccagc gcctgcccgc
      721 gccagcctgt tctccgataa ccagtggtcg tgatcacgac cactggttat cggagaacag
      781 gtagtgtgcg gcgggacgtg ttcggcgtga aggggacggg gcagcgatgg gtaagggcga
      841 aatcgtcgag tgtgaacggg tggatctggg gttcctctcg gacgccccga tcaaggtgac
      901 ggtgaatcag gtcgtcgcct gcaccggaca acgactctgg gacattctcg ccgacggcga
      961 gtcgtggacc cgctgggtca agccgatcgt cggggtcgaa tggacctcgc cgaaaccgct
     1021 cggcatcggc accacccgaa ccgtgaccat ggtcggcggc ctcgtcggct acgaggagta
     1081 cctcgcgtgg gatgcgcccc gtcatttcgc cttccggttc aaccagacca ccaagggcgg
     1141 cccggaagcc ttcgtcgagg agtaccgcat ctacgacctc ggcaacggaa gctgcaaggt
     1201 gtcctggacg atggcgatgc ggatcggcgg cccgtcggcc cggatgccgg gggtcgtcgc
     1261 tgcgggcatg aaaaaggcga acgccagctt cctcaagaag ctggcgtcct atgccgcatc
     1321 gaacccggtg cttgccgacg actgatcaac ccacgggggt gaacgtggcg ggcagcgagt
     1381 gaatgccggc cacgaaggcg gagggtgagc ggtcgatcgg gccgtgcgga tcgctcaacg
     1441 tgatgtccgg cagtcgccgg aacagttcct cgaagaccac cttgatctcc atgcgggcga
     1501 ggttggatgc catgcagtag tgggtgccga acccgaatgc gacgtgcgga ttcgggttcc
     1561 ggtcgatccg gaactcctcc ggattgtcga acacccggtt gtcgcggttg gccgactgat
     1621 agagcatgaa caccacatcg ccttcgacga ggtcgacccc gtgcagtgtg atcggcttcg
     1681 ccacggtgcg ggtgaagttc atgaccgggc tcgtgaagcg gacgatctcc tcgaccgccg
     1741 agtcgatcag atcggggttc gcgatcagca ggtcccgctg atccgggttc tggctgaagg
     1801 cgcgcattcc gccggcgatg gcgttgcggg tggtctcgtt gccggcgacc atcaacagca
     1861 cacagaacat cagcagctca tcgctgctga gatccgaaac cgtttcaccg ccctcgtaat
     1921 tctgaaggtg gccctcctcg tatttcagct ccccggcgtc gtaggcagcg gtgaggatgg
     1981 agatgagatc ctcgccaggg tcggctcggc gggcctcgat cagcccggcg atgtggctgg
     2041 tgtatccctc gaatgcctcg gcacccgcca tgagcgcttc gacgtcgtcg gggcccgccg
     2101 ct
//
LOCUS       DAOOMA010000518         2098 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_66523_length_2098_cov_4.413607, whole genome shotgun sequence.
ACCESSION   DAOOMA010000518 DAOOMA010000000
VERSION     DAOOMA010000518.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 2098)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2098)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2098
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66523_length_2098_cov_4.413607"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1388
                     /locus_tag="PLP95_11970"
     CDS             <1..1388
                     /locus_tag="PLP95_11970"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015094.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="AarF/ABC1/UbiB kinase family protein"
                     /protein_id="HPB46563.1"
                     /translation="GWVTTKHFGPLGVRSAMGRALKREVAPDAFARPLRKTFEELGTT
                     FMKFGQLVGSSPGVFGEEVAAEFRSCLDTGPVVAFDEVRRVIESELGMHLDEAFATFD
                     PDPIGRASIAVVHRATLHDGTDVAVKVLRPGIEERVAVDMDLFEPLLTLAARTTGEYA
                     AGQLVQMFDGFRLQLGEELDLRNEARAIRHMQGLLGVVDLPLVTVPTTYENFSGARVL
                     TMEFFDGVPVDDLERIAEYGFDPAPVVTQTVKGFLMTAVRWGFFHGDVHAGNLLLLRD
                     GRVAVIDWGICGRLDADTHHFFRRICEAGLGDDTAWADIAAHTIKQYGALLEEGLGMG
                     HDDVTAFMKLTLEPLLRAPFGEVSLVAILNAPQAKVAEAKGLEVERRSVRSVLKRFRE
                     QRKLRLMAEAEGIIESDFERGLFLLTKALMYFERYGKMYMNDVSIFGDEEFFRAALDA
                     PAETLREVNGR"
     gene            1463..2065
                     /locus_tag="PLP95_11975"
     CDS             1463..2065
                     /locus_tag="PLP95_11975"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HPB46564.1"
                     /translation="MVSASTGTRRTQAERREATRSAILNASLEQLLDGGLSGFTTTEV
                     CRRAGVSQGALFKHFDSKSALLAAVIEHLFEALRDDYEVAFVELGDAAQPAHGVDLLW
                     ARMFDPRLGAGFELYTAARSDADLRAALEPVVSSHCQRIHELAAGLLPEIDPQRLAPL
                     VDLAILSIQGLVLNQMAAPDPGQVERLRNMLDELVEFVVS"
BASE COUNT          349 a          660 c          705 g          384 t
ORIGIN      
        1 tcggttgggt caccaccaaa cacttcgggc cactcggtgt gcgcagcgcg atgggtcgtg
       61 cgctcaagcg cgaggtcgct ccggacgcct tcgcccgtcc tctgcgcaag accttcgagg
      121 agttgggaac gacgttcatg aagttcggcc aactcgtcgg atcgtcgccc ggggtgttcg
      181 gcgaagaggt cgcggccgag ttccgatcct gtctcgacac cgggccggtc gtggccttcg
      241 acgaggtccg tcgcgtgatc gagtccgaac tcgggatgca cctcgacgag gcgttcgcga
      301 ccttcgatcc cgatccgatc ggtcgtgcat cgatcgcggt cgttcaccgc gccacgttgc
      361 acgacggcac cgacgtggcc gtcaaagtgc tgcggccggg aatcgaagaa cgcgtcgcgg
      421 tcgacatgga cctgttcgag ccgctgttga ccctcgcggc gcgcacgacg ggggaatacg
      481 cggcggggca gctggtgcag atgttcgacg ggttccggct gcagctcggc gaggaactcg
      541 acttgcgcaa cgaggcccgg gcgattcgcc acatgcaggg gctgttggga gtggtcgacc
      601 taccgcttgt gaccgtgccc acgacctatg agaacttcag cggtgctcgg gtgttgacca
      661 tggaattctt cgacggtgtt cccgtcgatg acctcgaacg catcgccgag tacgggttcg
      721 atccggcgcc ggtcgtcacc cagacggtca aggggttcct catgaccgca gtacggtggg
      781 ggttcttcca cggagacgtc cacgccggga acctcctgtt gttgcgcgac ggccgtgtgg
      841 cggtgatcga ttggggcatt tgtggtcgcc tcgacgccga cacgcaccat tttttccggc
      901 gcatctgcga ggcgggcctc ggcgacgaca cggcgtgggc cgatatcgca gcgcacacga
      961 tcaagcagta cggagccctg cttgaagagg gcctcggcat gggccacgac gacgtgacgg
     1021 cgttcatgaa gctgaccctc gaaccgctgc tgcgggcgcc attcggtgag gtgtcgctcg
     1081 tggcaatcct gaatgctcca caggcgaagg tggcagaggc gaaggggctg gaggtcgagc
     1141 gtcggagcgt gcgttctgtc ctcaagcgtt tccgggaaca gcgcaagttg cgcctgatgg
     1201 ccgaggccga gggcatcatc gagagcgact tcgaacgtgg cctgttcctg ttgaccaagg
     1261 cgctgatgta tttcgagcgc tacggaaaga tgtacatgaa cgacgtcagc atcttcggcg
     1321 acgaggagtt cttccgcgcc gcgctcgacg ccccggctga gacgctgcgc gaggtgaacg
     1381 gtcggtagct cagggacgga atccaggcga cccatttaga aagtgacttg tcactttcta
     1441 aatgggggcg tacggtgagg ccatggtctc cgcctccact ggaactcgac gcacccaggc
     1501 cgaacgtcgc gaggcgacgc gctccgcgat cctcaacgcc tcgctcgaac agctcctcga
     1561 cggaggactc tcgggcttca ccacaaccga ggtctgtcgg cgtgccgggg tcagccaggg
     1621 ggcgttgttc aagcacttcg actcaaagtc cgcgctgctc gccgcagtca tcgaacacct
     1681 ctttgaggcg ctgcgtgacg actacgaggt ggcgttcgtc gaactcggcg acgccgccca
     1741 gccggcgcac ggcgtcgacc tgttgtgggc gcgcatgttc gacccccgcc ttggcgccgg
     1801 attcgagctc tacaccgcag cgcgcagcga tgccgatctg cgcgccgcac tcgagccggt
     1861 cgtgtcgtcc cactgccagc ggatccacga gctcgccgcc ggcctactcc ccgagatcga
     1921 cccccagcgc ctcgccccgc tcgtcgactt ggcgatcctg tcgatacagg ggctggtgct
     1981 gaaccagatg gccgctccgg accccggtca ggtggagcgg cttcgcaaca tgttggacga
     2041 gctcgtcgag ttcgtcgttt cgtaacggac caagggggga accgtgttgg aggaactg
//
LOCUS       DAOOMA010000519         2095 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_66702_length_2095_cov_3.125980, whole genome shotgun sequence.
ACCESSION   DAOOMA010000519 DAOOMA010000000
VERSION     DAOOMA010000519.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 2095)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2095)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2095
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66702_length_2095_cov_3.125980"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..448)
                     /locus_tag="PLP95_11980"
     CDS             complement(<1..448)
                     /locus_tag="PLP95_11980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB46565.1"
                     /translation="MGTARFSRSDGSVKCRPLETVELSIRGVRWAAIALVTAWMASSI
                     GRLDVTTALTALVAPLALLAANLLSVRTARSSSVSRLIWLQLFFGVVAVVAMTLAIRP
                     GGGSPMWAVIAFPVLEGAMRFQFGGAVMCWIAVATPLFIDRIMRWQP"
     gene            complement(452..1069)
                     /locus_tag="PLP95_11985"
     CDS             complement(452..1069)
                     /locus_tag="PLP95_11985"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:TIGR02228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HPB46566.1"
                     /translation="MALESVSVFGVVTMSMLCLWVVVPVVVLGWSASAIESGSMRPSI
                     ERGDVVLLRPLDRDQSLDPGAIIRFRSGGVGDSIIHRVSDVDLDGERYVTQGDANRSV
                     DSDLVPFDEVEGVGALMIPIVGFPMVWIAEGSYWSAALVFVGVLVLLTPVLRPTSPRK
                     GSSSLDSIDRGSVALTAWSFVPNDSRTLTLVPAAEGRAVGSEREV"
     gene            complement(1186..1767)
                     /locus_tag="PLP95_11990"
     CDS             complement(1186..1767)
                     /locus_tag="PLP95_11990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB46567.1"
                     /translation="MSSNHRNRVVTLVATAVSFIFVTFLIVGTSRAAFSDTTANDNNT
                     VGSAGVVLTDDDSGSAMFTVADMSPDDTIVRCIEVTYDGTITTNDPIQLYRTAAPTGT
                     GLEAYLDMTVEIGTGGGSAACTGFTPSSTLTSGSLAAFFSTHTAYASALSTAWTPTGA
                     GQKRTFRFTLTMQDDNAAQAKNTTFGYTWEARA"
BASE COUNT          414 a          694 c          665 g          322 t
ORIGIN      
        1 cgggctgcca gcgcatgatc cgatcgatga acaacggtgt cgccacggcg atccagcaca
       61 tgaccgcgcc gccgaactgg aaccgcatcg ccccctcgag cacgggaaag gcgatcaccg
      121 cccacatcgg gctgccgcct ccggggcgaa tggccaacgt catcgccacg accgctacga
      181 ctccaaagaa caactggagc catatcagcc gcgacaccga cgacgaccgt gccgtgcgca
      241 ccgacaacag gttcgccgcc aacaacgcca acggagcgac gagtgccgtc aaggcggtcg
      301 tgacgtcaag acgtccgatc gaggacgcca tccatgccgt gacgagcgcg atcgccgccc
      361 accgaacacc ccgaatcgac aattcgacgg tctcgagcgg ccggcatttg accgaaccgt
      421 cgcttctgga gaaacgggcc gtccccatgg ctcacacttc ccgttccgag cccaccgctc
      481 ggccttcggc tgccggaacg agcgtgaggg tccgtgagtc gttcggaacg aatgaccacg
      541 cggtcagtgc gaccgaaccg cgatcgatcg agtcgagcga cgatgatcct tttcggggcg
      601 acgtcggccg caacacgggc gtgagcaaca cgagcacgcc gacgaagacg agcgcggcgc
      661 tccagtacga gccctcggcg atccacacca ttggaaatcc gacgatcgga atcatgagcg
      721 caccgacccc ttcgacctcg tcgaacggca ccagatcgct gtcgaccgag cgattcgcat
      781 ccccctgagt gacgtaccgc tcgccgtcga ggtcgacatc ggaaacccgg tggatgatcg
      841 aatcgccgac ccctcccgat cggaaccgga tgatcgcccc cggatccagg gattggtcgc
      901 ggtcgagggg tcgaagcaac acgacgtcgc cgcgttcgat cgacggccgc atcgaacccg
      961 actcgatcgc ggaggccgac cacccgagca ccacgaccgg caccacgacc cacaggcaca
     1021 gcatcgacat cgtcacgacc ccgaacaccg acaccgattc caacgccatc ttcgaatcgc
     1081 ctcgcaccac ctcgatgacc gctgcggtcg acgatctggc gaggcgattc gatcgccggg
     1141 acgggggaca tggagcgaga cgtcgtcgac gcagcggcga tgccgctagg cgcgggcttc
     1201 ccaggtgtag ccgaacgtcg tgttcttcgc ctgggccgcg ttgtcgtcct gcatcgtcag
     1261 ggtgaacctg aaggtgcgct tctgcccggc tccggtggga gtccacgccg tcgacaatgc
     1321 gctggcgtag gccgtgtgag tgctgaagaa cgcggcaagc gatccgctcg tcagggtcga
     1381 agacggggtg aacccggtgc atgcagccga acccccaccc gtgccgattt cgacggtcat
     1441 gtcgaggtag gcctcaaggc cagtgccggt cggagcagcc gtgcgataga gctgaatggg
     1501 gtcattcgtg gtaatcgtgc cgtcgtaggt cacctcgata caccgcacga tggtgtcgtc
     1561 ggggctcatg tcagcgacgg tgaacatcgc cgaccccgag tcatcatcgg tgaggacaac
     1621 cccggccgaa ccgacggtgt tgttgtcgtt cgccgtggtg tcggagaacg cggcacgtga
     1681 ggttccaacg atgaggaacg tgacgaagat gaacgaaacg gcggtggcga cgagggtgac
     1741 gacgcgattg cgatggttgc ttgacatggt gtcctcagag gggtgggtcg cgtagcggca
     1801 tggacatcat gtagatcggc atcgaggcgt cgcggctgag tgcaccggtc gaaattccca
     1861 accgaggtag ccctgatgac ccaggcgaac gcgcgagccg gttttcgacc gctgacaact
     1921 caacaggacc gccgcaccgc cgatgagcat tcagatgaat cgcctctgga tctcgcgggt
     1981 gttgctgggc tggctgttgg tcgtcggcgc gctcaccgtc gctacgcgca ccgtgtcggt
     2041 cgccgcattc tccgacacca ccgacaccgg cgcctcgagt gctgccgggg cgctc
//
LOCUS       DAOOMA010000520         2092 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_66838_length_2092_cov_2.684340, whole genome shotgun sequence.
ACCESSION   DAOOMA010000520 DAOOMA010000000
VERSION     DAOOMA010000520.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 2092)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2092)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2092
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66838_length_2092_cov_2.684340"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..747)
                     /gene="mobF"
                     /locus_tag="PLP95_11995"
     CDS             complement(<1..747)
                     /gene="mobF"
                     /locus_tag="PLP95_11995"
                     /inference="COORDINATES: protein motif:HMM:NF041492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MobF family relaxase"
                     /protein_id="HPB46568.1"
                     /translation="MSIGKLAAGAGDYYTAMVADGAEEYFTSAREAPGVWVGQASQRL
                     GLAGTVQPDDFASVLDHQYPGTNVRITAARSAPSVAGFDATFCAPKSVSILHGLGTPD
                     IRAAVRQAHDVAVREALAVFESAASRGRRGHGGADVIDGDGFVAAAFAHRTSRAGDPH
                     LHTHVVIANLVHGPDGRWTALDARPLYHWSKTVGYLYEAELRHQLTRTVGVEWRTVRR
                     GIADVAEVPMQVVDEFSTRRHEIEAHLAENG"
     gene            complement(863..1216)
                     /locus_tag="PLP95_12000"
     CDS             complement(863..1216)
                     /locus_tag="PLP95_12000"
                     /inference="COORDINATES: protein motif:HMM:NF024139.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HPB46569.1"
                     /translation="MSLPELFTVTEAAVVLRIGRTTAYELARRDLATGGGEGLGVIRV
                     GGQLRVPRVALERLIGAPVGTPVTTGPTVPLVPDTVVPLDVVPDPTPARVDHPSAASG
                     SRRRLVSVDQLRFDS"
     gene            1528..1878
                     /locus_tag="PLP95_12005"
     CDS             1528..1878
                     /locus_tag="PLP95_12005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HPB46570.1"
                     /translation="MPSRPKRLTPNQIVAFNLTRARTDRGWSQPEAAAALEPFLGVRW
                     SRASYSAVERSVDGVRIKQFSADELVAIARCFDRPVSWLLTPPAGTVAATPDHLDGLD
                     ASVLQRTLRSTRKP"
     gene            1875..>2092
                     /locus_tag="PLP95_12010"
     CDS             1875..>2092
                     /locus_tag="PLP95_12010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB46571.1"
                     /translation="MTEPTPERNRRAPSVAPRKNPTTGLWEFIVDLGPGADPGGVWRE
                     RRQARRRGFATKAAAQEALDDLRGKARTG"
BASE COUNT          363 a          739 c          686 g          304 t
ORIGIN      
        1 cccgttctcc gccaggtgtg cttcgatctc gtgacggcgg gtagagaact cgtcgaccac
       61 ctgcatcggc acctcggcga catcggcgat cccccgccgc accgtccgcc actcgacacc
      121 gacagtccga gtgagttggt gacggagctc ggcctcgtac aggtagccga ccgtcttcga
      181 ccagtggtag agcggtcggg cgtcgagggc ggtccaccgc ccgtcgggcc cgtggacgag
      241 gttggcgatc accacgtgtg tgtgcaggtg gggatcgccg gcgcgagagg tgcggtgagc
      301 gaacgcagcg gcgacgaacc cgtcgccatc gatcacgtcg gctccgccat ggcctcgccg
      361 gcctcgcgac gctgcggact cgaacaccgc gagcgcttca cggacggcga cgtcgtgcgc
      421 ctggcggacg gcagcacgga tgtcgggggt gccgaggccg tggagaatcg acaccgactt
      481 cggagcgcag aacgtcgcgt cgaacccggc gacgctcggc gcggaccgag ccgctgtgat
      541 ccgcacgttg gttcccggat actgatgatc gaggacggat gcgaagtcgt cgggctggac
      601 ggtgccggcg agtcccagcc gttggctggc ttgaccgacc cagacgccgg gggcctcgcg
      661 ggccgaggtg aagtactcct cggcgccgtc ggcgaccatg gcggtgtagt agtcgccggc
      721 cccggcggcg agcttgccga tgctcaacaa ggcgaccgcc gacctgaaca gcggacgtgt
      781 ccgaccacag ggcaccgtgg tgcaagtgcg tcgaacgaaa tggcacctga ccaaccaaca
      841 gtcctgccga ccgcgcgacc gatcacgaat cgaaccgcaa ctgatccacc gacaccaacc
      901 gacgacgact gccagacgca gcactcgggt ggtcgacgcg agcaggcgtt ggatcgggga
      961 cgacatcgag cggcaccacc gtgtcgggca ccaacgggac cgtcggcccg gtggtgaccg
     1021 gtgtcccgac cggggcgccg atcagccgct caagggccac cctggggacc cggagctgcc
     1081 ctccgacccg gatcaccccc aagccctccc cgcccccggt ggcgaggtcg cgtctggcga
     1141 gctcgtaggc ggtggtccgg ccgatgcgca gcacgacggc cgcctcggtg acagtgaaca
     1201 attcaggtag tgacatctgc gcttctccaa gggttgaggt gtttccctac ctatgcgcgc
     1261 gcgggtcccg ccgaaggcga tatctgtcga atcctcccga gggaacgaaa ctgtccagtc
     1321 ggccgtccac cggaccgtcg tgttcactgt cgcctctcct gtcgccacgc tgacggcgtc
     1381 gaatccggcc attccgatgg tcatcctgac caccgacctg aacaatcttg cgttcaggga
     1441 tgaccatcac cgattccgga ctttgacgcg tacgtgtcaa ctttttcgtt gatgacagcc
     1501 ggacgccgca agcgtagagt cggcgacgtg cccagccgcc cgaagcgcct cacgccgaac
     1561 cagatcgttg cgttcaacct cacgcgtgcc cgcaccgacc gcgggtggag ccaacccgaa
     1621 gccgccgcgg cgctcgaacc gtttctcggc gtgcgctggt cgcgagcgag ctactcggcc
     1681 gtcgagcggt cggtcgacgg tgtccgcatc aaacagttct ccgccgacga actcgtcgca
     1741 atcgcccgct gtttcgaccg gcccgtcagc tggttgctca ccccacccgc cgggacagtg
     1801 gcagccacgc ccgaccacct cgacggcctc gacgccagcg tcctccaacg aacactccgc
     1861 tcaacgagga aaccgtgaca gaaccgacac ccgagaggaa ccggcgcgca ccatcggtcg
     1921 ctccacgcaa gaacccgacg actgggctat gggagttcat cgtcgacctc ggtcccggcg
     1981 ccgaccctgg cggggtctgg cgggagcgac gccaagcgcg acgacgcggc ttcgccacca
     2041 aggctgccgc ccaggaggcg ctcgacgacc tccgaggcaa ggcacggacc gg
//
LOCUS       DAOOMA010000521         2084 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_67181_length_2084_cov_2.918679, whole genome shotgun sequence.
ACCESSION   DAOOMA010000521 DAOOMA010000000
VERSION     DAOOMA010000521.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 2084)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2084)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2084
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67181_length_2084_cov_2.918679"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..710)
                     /gene="recA"
                     /locus_tag="PLP95_12015"
     CDS             complement(<1..710)
                     /gene="recA"
                     /locus_tag="PLP95_12015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015798102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HPB46572.1"
                     /translation="MEQHKALEGALAQIEKQFGKGSVMRMGEKTSMAVGSVSTGALSL
                     DIALGIGGLPRGRVTEIYGPESSGKSTLAMHVVAEAQRNGGTCAYIDAEHAMDPAYAA
                     RIGVDVDQLLISQPDTGEQALEITDMLIRSGALDVIVIDSVAALTPRAEIEGEMGDSH
                     VGLQARLMSQALRKVTANLNKTDTICIFINQLREKIGVMFGSPETTPGGRALKFYSSV
                     RLDIRRIESIKDGVEIVG"
     gene            complement(983..>2084)
                     /locus_tag="PLP95_12020"
     CDS             complement(983..>2084)
                     /locus_tag="PLP95_12020"
                     /inference="COORDINATES: protein motif:HMM:NF014517.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="nicotinamide-nucleotide amidohydrolase family
                     protein"
                     /protein_id="HPB46573.1"
                     /translation="HDDLTRSAIAAVMGVELEFDADRADLIRSMFGGRGRDMPDNNLL
                     QAYKPVGAQFIEQQPGTAPGLVCPVAWPRDGARRGAGGGGGGGGAESVPTVDKVIYAV
                     PGVPWEMKEMLAATIVDDLRRRSGEHFVIGSRVLRTWGQSESGLAELLADRIAELDEL
                     GNPTIALLASGAEGLKVRVTAKAPTEQEATAILDEEETRLRRILSELVFGIDDQTMES
                     VVLDLLRERGDTLAIAESVTGGLVSSRLVDVPGASAVFRGGVVSYASEVKFDVLGVPE
                     GPVVTLEAAEAMATGVRRVLGATVGVGITGVAGPDPAEGRRPGTVCIAVDLNGAVSSA
                     EMRLPGGRQQVRELTCISALAMLRTRLATADT"
BASE COUNT          372 a          724 c          633 g          355 t
ORIGIN      
        1 ttgccgacga tctcgacacc gtccttgatg gactcgatac gacgaatgtc gagacgcacc
       61 gagctgtaga acttcagtgc acgaccgccc ggggtggtct ctggcgagcc gaacatcacg
      121 ccgatcttct cgcgaagctg gttgatgaag atgcagatgg tgtcggtctt gttgaggttc
      181 gccgtgacct tacgaagggc ctggctcatg aggcgggcct gaagacccac atggctgtct
      241 cccatctccc cctcgatctc ggcccgaggc gtgagcgcgg ccaccgagtc gatgacgatc
      301 acgtcgagtg ccccggaacg aatcaacatg tcggtgatct cgagcgcctg ctcgccggtg
      361 tcgggctggg agatgaggag ttgatcgaca tcgacgccga tgcgggccgc atatgccgga
      421 tccatcgcat gctcggcgtc gatgtaggcg catgttccgc cgttgcgctg agcctcagcg
      481 accacgtgca tggccaaggt ggacttgccg gaggactccg gaccgtagat ttcggtgacg
      541 cggccacgcg gcagcccacc gattccgagt gcgatgtcga gactcagagc gccggtcgag
      601 accgagccga ccgccatcga cgtcttctcg cccatccgca tgaccgagcc cttgccgaac
      661 tgcttttcaa tttgtgccaa cgcgccttcg agtgccttgt gctgctccac gtctgtttcc
      721 ttccaactgg ttcgtggtgg gctgccgtgt tggcagcgat atcgatgcaa tgaccgtacc
      781 gatgacccct gacagcgaag ccggggaact cacacgaacg tagtttcgaa caggtgttct
      841 tggcaagcat ttacgccaac atttcttccc catgccatct cgggagcagc cgccgagcag
      901 ccgcccgccc gttctccgat aaccagtggt cgtgatcacg accactggtt atcggagaac
      961 gggcaagggc acgcgagaac gatcaggtgt cggcggtggc cagtcgcgtc cgcaacatgg
     1021 cgagcgcgga gatgcaggtc aactcccgta cctgctgacg gcctccgggc agacgcatct
     1081 ccgcggagga caccgcgccg ttgagatcga cagcgatgca caccgtgccg ggccgtcgcc
     1141 cctcggccgg atcggggccc gcgactccgg tgatgccgac acccacggtc gcccccaaca
     1201 cccggcgcac acccgtggcc atcgcctcgg cagcctccag ggtcaccacc ggaccttccg
     1261 gaactcccag cacgtcgaat ttcacctcgg aggcatacga caccaccccg ccgcgaaaga
     1321 ccgccgaggc acccggtacg tcgaccagcc gcgacgacac caacccaccg gtcaccgact
     1381 cggcgatcgc caaggtgtcg ccgcgctcgc gcaacaggtc gagcaccacc gattccatgg
     1441 tttggtcgtc gatgccgaac accaactcgc tgaggatgcg tcgcagccgc gtctcctcct
     1501 cgtcgaggat cgcggtcgcc tcctgttcgg tcggtgcctt ggcggtcacc cgcaccttga
     1561 ggccctccgc gcccgaggcc aacagcgcga tcgtcgggtt gccgagctcg tcgagttcgg
     1621 cgatccggtc ggccaacaac tcggcgagcc ccgattcgga ctgaccccag gttcgcagga
     1681 cgcggctacc gatcacgaaa tgctcaccac tgcggcgacg aagatcgtcg acgatcgtgg
     1741 cggcgagcat ctccttcatc tcccacggca cgccgggcac cgcatagatg accttgtcga
     1801 cggtcgggac ggactctgca ccgccaccgc caccgccgcc acccgcgccg cggcgagcac
     1861 cgtcacgtgg ccaggcaacc ggacacacca gacccggcgc cgtgccgggt tgttgctcga
     1921 tgaactgcgc cccgaccggt ttgtaggcct gcaacaggtt gttgtcgggc atgtcgcgtc
     1981 cgcgtccacc gaacatcgaa cggatcaggt ccgcacgatc ggcgtcgaac tccaactcga
     2041 cccccatcac cgcggcgatc gccgagcggg tgaggtcgtc gtgg
//
LOCUS       DAOOMA010000522         2082 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_67260_length_2082_cov_3.474100, whole genome shotgun sequence.
ACCESSION   DAOOMA010000522 DAOOMA010000000
VERSION     DAOOMA010000522.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 2082)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2082)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2082
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67260_length_2082_cov_3.474100"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            155..433
                     /locus_tag="PLP95_12025"
     CDS             155..433
                     /locus_tag="PLP95_12025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB46574.1"
                     /translation="MEGDRVLTMMWTVVRSLARHPRLWPTAFAQFLVLVPPRWWRRRP
                     WLPVPDVAYLRFRMVTAYGGSGDARATSEDVIAYLQWCRSMRVLSSAG"
     gene            complement(444..935)
                     /locus_tag="PLP95_12030"
     CDS             complement(444..935)
                     /locus_tag="PLP95_12030"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HPB46575.1"
                     /translation="MGYVIRRIAARVVCFNPQGQLLMIRSSDPVDRTKPDWWELPGGG
                     IDHGESATDAILRELREECGVNNVVMGQLIWTQHAQFTFAGWDFDQHETLFVAQSDGD
                     SSGVTTLEALEAVAFQELRWRTIEELMADPLPTVPPRLREFIVPISRGDYPNEPIDIS
                     PRT"
     gene            977..1675
                     /locus_tag="PLP95_12035"
     CDS             977..1675
                     /locus_tag="PLP95_12035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB46576.1"
                     /translation="MSFSVERRRGRGEDLHATGFIEARRSVFLMELEGACAVLGSTQS
                     AHILDPDAVERSGRQVTTRRSGGGLVVMNPGEVLWVDVVIPAGDPLWRDDVSSAGAWL
                     GELWVDALHDAGEIADSGSFEVHRGPLRHRDLARTVCFAGIGAGEVVDGSAKVIGVSQ
                     RRTRHWARFQCLAYARFDPTETTGLLDDAVFGGPTSSVDRDALTRHLSASVRTVPAMD
                     RVSDALVERLVTLR"
BASE COUNT          311 a          634 c          755 g          382 t
ORIGIN      
        1 cattgccgga gaagtcttgc aggtcgctcg gtaggatggc gttcgtgccg acccaggagt
       61 tcccgtcaac cagcgcgtca accagtgcgt taaccagtgc gtcaaccagc gcgtcgagcg
      121 gcgcgtcgac ggcgccgacc cgtacccgca gtccatggag ggtgaccggg tgttgacgat
      181 gatgtggacg gtcgtgcggt ccttggcgcg ccacccccgg ctgtggccga cggcgttcgc
      241 ccagttcctc gtgctggtgc cgccgcggtg gtggcgtcgg cggccgtggc tgccggtccc
      301 cgatgtcgcc tacctgaggt tccggatggt gaccgcctac ggcgggtcgg gcgatgcccg
      361 cgcgacgagc gaggatgtca tcgcctacct gcagtggtgc cgttcgatgc gggtgttgtc
      421 ctcggccggg tgacgcggat cgatcacgtc ctcggcgaga tgtcgatcgg ttcgttcggg
      481 tagtcgcccc gagagatcgg cacgatgaat tcgcggagtc gtggcggcac ggtcgggagg
      541 ggatcggcca tcagctcttc gatggttcgc cagcgcagtt cctgaaacgc gacggcctcg
      601 agggcctcga gggtggtgac gccgctcgaa tccccgtcgg attgcgcgac gaacagtgtt
      661 tcatgctgat cgaagtccca gccggcgaag gtgaactgag cgtgctgggt ccagatgagc
      721 tggcccatga cgacgttgtt cacgccgcat tcctcgcgca gctcgcgaag gatcgcgtcg
      781 gtcgcgctct cgccgtggtc gatgccacct ccgggcagtt cccaccagtc gggcttggtg
      841 cggtcgacgg ggtcgctcga acggatcatg agcagttggc cctgcgggtt gaaacagacc
      901 acgcgtgcgg cgatgcgtcg gatcacgtat cccatggcgc cgaaccatac ttgccccgcc
      961 ggtagcattc gccccgatgt ctttcagcgt tgaacgccgc cggggtcgtg gcgaggacct
     1021 tcacgctacg gggttcatcg aggcgcgacg cagcgtcttt ctcatggaac tcgagggcgc
     1081 atgcgcagtg ctcggcagca cccagtcggc gcacatactc gatccggatg cggtggagcg
     1141 cagcggccgt caggtcacca ctcggcgctc cggcggcggg ctcgtggtga tgaacccggg
     1201 ggaggtcctg tgggtggatg tggtgatccc cgcgggcgat ccgctgtggc gcgacgacgt
     1261 ttcgtcggcc ggggcctggc tgggggagtt gtgggtcgac gcgctccatg acgccgggga
     1321 gatcgccgac tcgggatcct ttgaggtaca tcgcggtccg ctccggcacc gtgacctcgc
     1381 ccgtacggtg tgtttcgccg gtatcggagc cggcgaggtc gtcgacggtt cggccaaggt
     1441 gatcggggtt tcgcagcgcc gaacgcggca ttgggcgagg ttccagtgtc tggcctatgc
     1501 ccggttcgac cctaccgaga cgaccgggtt gttggacgac gccgtgttcg gtggcccgac
     1561 gagctcggtg gaccgcgacg cgctcacccg gcacctgagc gcgagcgttc ggacggtacc
     1621 ggcgatggat cgcgtgtcgg acgcgttggt cgaacgcctc gtcaccctcc gctgacgttt
     1681 cgcggcagcg tcgccggtcc ggccccgctc caccggtggc gcgttcgacc ggtggcgcgg
     1741 gctgccggtg aggtcgattt ggcgcgaagt ggagcggatc gaagcctgga cgggctcgga
     1801 acgctcgttc gaaatcacaa acgtggaagg ggatttcatg atgtctgaat gatgttcaat
     1861 gcatctccaa cgcgaaaaaa cgcgagaaat ctcccaaaaa ccggcgacga acgctagcgg
     1921 tggtggggtg gtcactgcgt agagtggtgc gttatggagt gccggaccag tgaagtggtg
     1981 gaaagtggtg gctgtgacag ctgccacggc cgcgctgcga caccggaggc ccgcgggggt
     2041 gcccgatgac ggcccttcgc acctacgtcg gcacccacgt ca
//
LOCUS       DAOOMA010000523         2067 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_68032_length_2067_cov_2.859841, whole genome shotgun sequence.
ACCESSION   DAOOMA010000523 DAOOMA010000000
VERSION     DAOOMA010000523.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 2067)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2067)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2067
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68032_length_2067_cov_2.859841"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            298..642
                     /locus_tag="PLP95_12040"
     CDS             298..642
                     /locus_tag="PLP95_12040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB46577.1"
                     /translation="MEVGEGGDEAREAGGDAAAEARWRHAPGWVWRAIPGRVLLAARG
                     GLERTVEGIAVPVWIALSEPATISELTGEFAEVWPDQPPSRDAISEALEVMATYDLVV
                     CDGVAQGPGARP"
     gene            653..1717
                     /locus_tag="PLP95_12045"
     CDS             653..1717
                     /locus_tag="PLP95_12045"
                     /inference="COORDINATES: protein motif:HMM:NF026257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HPB46578.1"
                     /translation="MNHQLASVAHPDDVMKALAVRGLRGAQVSVADLAPIDHLDPRSL
                     VGVAEATRMLGPMLGAVHDGELDFDGDSTRYLVERHEANMLWAIKLEARILEMRDLFA
                     AAGGVRHLILKGSAIAHLDEDDPAMRSTSDVDVLVAGDDLDRAVGALSADGATRQFPQ
                     RRPGWDRTFAKSVTMTGTDAIEIDLHRMLCDGVFGVRLPLAELFAESTTFELGGERVG
                     ALSRQHRFLHAAWHAVLGSRHPKLLNLRDLARYMDSPALAPDAIAPIARHWGGEAVLV
                     TAIREVHDALPFQSPTWTDWAQQATPTIPQREFELVERHRNEGSSFGRAKVDMWRELP
                     SLRLKSAYAWGAVVGRILRP"
     gene            complement(1825..>2067)
                     /locus_tag="PLP95_12050"
     CDS             complement(1825..>2067)
                     /locus_tag="PLP95_12050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB46579.1"
                     /translation="AIVHHDPGGDGWHPISHAQATIEVLSHTICSRSRPGDALDAVLK
                     IASGAESVLGSRGEASEAIVELLELLEGRGEGTARE"
BASE COUNT          366 a          714 c          672 g          315 t
ORIGIN      
        1 gatcatccac accatctggc gatgcatcgc gtccttgaac tccgaccgca gcttctccat
       61 ctcaaggcga agctccgcgc tgagcaacgc gagatccgtc ttcgtcgcga cgtcacccca
      121 gcccagcggc ggaagatgct ccatcaacgt ctccgctcta tcaaaagctc aagagggaac
      181 cgagttccga gaaccactcc gtccatcaac caaagaaggc ggcggcgcct cccaacgtac
      241 ccagcgaatg gcaccgcgac aacccatctg cgatgactcc tgtggcaggc tggcgaggtg
      301 gaggttggcg agggcggcga cgaagcacgc gaggccggcg gtgatgctgc agcagaggct
      361 cggtggcgac acgccccggg ttgggtgtgg cgcgccatcc ccgggcgggt gctgctcgcc
      421 gcacgtggcg ggctcgagcg caccgtcgag ggcatcgcgg tgccggtgtg gatcgcgttg
      481 agcgaaccgg cgacgatcag cgaattgacc ggggagttcg ccgaggtgtg gcccgatcag
      541 ccgccgagcc gagacgccat cagcgaggcg ctcgaggtga tggcgaccta cgacctggtc
      601 gtatgcgacg gggtggccca agggccgggg gcccgaccat gagcggccga ccatgaacca
      661 ccaactggcg tcggtggccc accccgacga cgtgatgaag gccctcgcgg ttcgcggact
      721 gcgcggggcg caggtatcgg tggccgacct cgcgccgatc gatcacctcg acccgcgatc
      781 gctcgtcggg gtcgccgagg caacccgaat gctcggcccg atgctggggg cggtccacga
      841 cggcgagttg gatttcgacg gcgactcgac ccgatacctc gtcgagcgtc acgaggcgaa
      901 catgttgtgg gccatcaaac tcgaggcccg catcctggag atgcgcgacc tgttcgctgc
      961 ggccggcggg gtgcgccacc tcatcttgaa aggctccgcg atcgcgcatc tcgatgagga
     1021 cgacccggcg atgcgctcga cctccgatgt cgacgtgttg gtcgccggcg acgacctcga
     1081 ccgcgcggtt ggcgcgctca gcgccgatgg ggccacacgg cagttcccac aacgccgtcc
     1141 gggctgggac cgaactttcg ccaagtcggt caccatgacc ggcaccgacg ccatcgagat
     1201 cgacctgcac cggatgctgt gcgacggcgt gttcggggtg cggctgccgc tggcggagtt
     1261 gttcgccgag tccaccacct tcgaactcgg cggcgaacgc gtcggcgcgc tctcgcgaca
     1321 acatcggttc ctgcacgcgg cctggcatgc ggtgctcgga tcgaggcacc cgaagctgtt
     1381 gaacctgcgc gacctcgccc gctacatgga ctcccccgcg ctcgcgcccg acgccatcgc
     1441 gccgatcgcc cgccactggg gcggcgaagc ggtgctcgtc accgcgatac gcgaggtcca
     1501 cgacgcgctc cccttccaat cgccgacgtg gaccgactgg gcacaacaag caacaccgac
     1561 aatcccacag cgagaattcg aactcgtcga acgccaccgc aacgaaggct cctcattcgg
     1621 ccgagccaaa gtcgacatgt ggcgcgaact gccgagcctg cgactgaaga gcgcgtacgc
     1681 ctggggcgcc gtcgttggac gaatactccg gccgtagcgc tgtcaataga gcgcgttgcg
     1741 gcccggccat gacaggtctt gcctcgtcta cgacaacgac agccacaacg acaacgtcat
     1801 cgggggcgct cgcacaatca gccgttattc gcgtgccgtt ccttcgccac gaccctcaag
     1861 gagctccagc agctcaacga ttgcttcgct ggcctcgccc cgactgccaa gaaccgactc
     1921 ggccccgctg gcgatcttca acacagcgtc gagcgcgtcg cccggtcgcg aacgactgca
     1981 gattgtgtga gagagcacct cgatcgtcgc ttgtgcatgc gagatcgggt gccagccatc
     2041 cccgccgggg tcgtggtgca cgatggc
//
LOCUS       DAOOMA010000524         2064 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_68150_length_2064_cov_5.158288, whole genome shotgun sequence.
ACCESSION   DAOOMA010000524 DAOOMA010000000
VERSION     DAOOMA010000524.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 2064)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2064)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2064
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68150_length_2064_cov_5.158288"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..838
                     /locus_tag="PLP95_12055"
     CDS             <1..838
                     /locus_tag="PLP95_12055"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR01930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003985 - acetyl-CoA C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HPB46580.1"
                     /translation="AGVNDLVVTGGVQNMSMIPISSAMTVAEPMGFTDPFCGSTGWVD
                     RYGTQEVSQFRGAELIAEKWEISREDMEGFAIESHTRAIRAREEGRFDAEIVEFNGLS
                     HDEGPREPNVDKIRSLQTLVEGGRITAAVASQISDASAAMLIASEQAVKDHNLTPRAR
                     IHHLSVRADDPIFMLSAPIPATRYALDKAGMSLDDIDLVEINEAFASVVLAWAKELDA
                     DLSKVNVNGGAIALGHPLGATGVRLMTTMLNELERTGGRYGLQTMCEGGGQANVTIIE
                     RL"
     gene            858..>2064
                     /locus_tag="PLP95_12060"
     CDS             858..>2064
                     /locus_tag="PLP95_12060"
                     /inference="COORDINATES: protein motif:HMM:NF012656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HPB46581.1"
                     /translation="MNFDDTPEEAAFRAEVQAFLAANAKPKAGDETDWSRNGASTDHA
                     VAEDYRRRCREWQRTLYDNGWAGIAWPKAFGGRGGNASQQIIFNQELAKYDATSGFIG
                     AAQALVGPAIMAFGTPEQQQRYLPPLLSGEESWCQLFSEPEAGSDLATLGARAVLDGD
                     EWVVNGQKVWNSSAQHADFGILICRTNPDVPKHAGITYFIVDMRTPGIEVRPLIQAQG
                     VAHFNEVFFSDVRIPAANVVGAVNDGWKVTKVTLRSESSMISGGGQASTFVNVLATIR
                     RTGRDTDPLVRQELAKVWSNEQILRYLSMRMQTAVMTGRKDLALHGSLLKNFFTRSFT
                     HRATLALDCEGPEGMLTGADAEGDGFWQYQCINQFASKIGGGTEEVHRNNLAEQALGL
                     PREASADVDT"
BASE COUNT          376 a          663 c          696 g          329 t
ORIGIN      
        1 ggcgggcgtc aacgacctcg tcgtcaccgg cggcgtgcag aacatgtcga tgattccgat
       61 cagctccgcc atgaccgtcg ccgaaccgat gggcttcacc gacccgttct gtggctcgac
      121 cggctgggtc gatcgctacg gcacccagga ggtgagccag ttccgcggcg ccgaactcat
      181 cgccgagaag tgggagatct cccgcgagga catggagggg ttcgcgatcg agtcgcacac
      241 ccgggcgatc cgggcgcgcg aggagggtcg cttcgacgca gagatcgtcg agttcaacgg
      301 cctttcccac gatgagggtc ctcgcgagcc gaacgtcgac aagatccgct cgctgcagac
      361 cctggtcgaa ggcggacgca tcaccgcggc ggtggcctcg cagatttccg atgcttcggc
      421 cgcaatgttg atcgcgtcgg agcaggcggt gaaggaccac aacctcaccc cccgcgctcg
      481 gatccaccat ctttcggtac gtgcggatga cccgatcttc atgctgtcgg ccccgattcc
      541 cgcgacgcgc tatgcgctcg acaaggcggg gatgtcgctc gacgacatcg acctcgtcga
      601 gatcaacgag gcgttcgcgt cggtggtgtt ggcctgggcg aaggagctcg acgcggacct
      661 gtcgaaggtg aacgtcaacg gtggagcgat cgcgctcggg cacccgctcg gtgccaccgg
      721 ggtgcggctc atgaccacga tgctgaacga actcgaacgc accggcggtc gttacgggct
      781 gcagaccatg tgtgagggcg gcggacaggc caacgtcacc atcatcgagc gcctctaggc
      841 atcgccggag acgacccgtg aatttcgacg acacccccga ggaagcggcg tttcgcgccg
      901 aggtgcaagc gttcctcgcc gcgaacgcca agccgaaggc cggggacgag accgactggt
      961 cgcgcaacgg agcctcgacc gaccacgccg tcgccgagga ctatcggcgt cggtgccgcg
     1021 agtggcagcg cacgctgtac gacaacggtt gggcgggaat cgcctggccg aaggcgttcg
     1081 gtggtcgggg aggcaacgcg tcccagcaga tcatcttcaa ccaggaactc gcgaagtacg
     1141 acgcgacgag tggcttcatc ggtgcggccc aggcgcttgt gggcccggcg atcatggcct
     1201 ttggcacccc ggagcaacag cagcgctacc tgcctccgct gctgagtggc gaggagtcgt
     1261 ggtgccagct cttcagcgag ccggaggccg gtagcgacct ggcgacgctg ggcgcgcgtg
     1321 ccgtgctcga cggcgacgag tgggtcgtga acggccaaaa ggtgtggaac agctccgcgc
     1381 aacacgcgga tttcggcatc cttatctgcc ggaccaaccc cgacgttccc aagcacgcgg
     1441 gcatcaccta cttcatcgtc gacatgcgca ccccgggcat cgaggtgcgt ccgctcattc
     1501 aggcacaggg cgtcgcgcac ttcaacgagg tgttcttcag cgacgttcgc attccggcgg
     1561 ccaatgtcgt cggggcggtg aacgacggct ggaaggtcac caaggtgacg ctgcgcagcg
     1621 agagttcgat gatctccggc ggtggtcagg cctcaacgtt cgtcaacgtg ttggcgacga
     1681 tccgccgaac cggcagggac accgatccgc tggtgcgaca ggaactcgcc aaggtgtggt
     1741 cgaacgagca gatcctgcgg tacctctcga tgcgcatgca gacggcggtg atgacgggtc
     1801 gcaaggatct ggcgctgcac gggtcgttgc tgaagaactt cttcacccgc agcttcaccc
     1861 atcgcgccac cctcgccctg gactgcgagg ggccggaggg catgttgacc ggagccgatg
     1921 ccgagggcga cggattctgg caataccagt gcatcaacca gttcgcctcc aagatcggcg
     1981 gcggaaccga ggaagttcat cgcaacaacc tcgccgagca ggcactcggg ttgccccgcg
     2041 aggcgtcggc cgatgtcgac accc
//
LOCUS       DAOOMA010000525         2062 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_68270_length_2062_cov_2.941704, whole genome shotgun sequence.
ACCESSION   DAOOMA010000525 DAOOMA010000000
VERSION     DAOOMA010000525.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 2062)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2062)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2062
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68270_length_2062_cov_2.941704"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            144..1316
                     /gene="dxr"
                     /locus_tag="PLP95_12065"
     CDS             144..1316
                     /gene="dxr"
                     /locus_tag="PLP95_12065"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HPB46582.1"
                     /translation="MTTIAVMGSTGSIGTQTLEVIATDPQRFQVDSLGAFGGNLDLLA
                     KQANTVRPRVVALADDAKAAELKERLDPGIELVAGPDSLAEVSTAADVTINGVVGFAG
                     LGVTMEVLRSGKRLGLANKESLIAAGPVVQPLRATPGAELIPVDSEHCAIHQCLRSGA
                     QGAGGPRLKRIVLTASGGPFRGRSLRELESVTVQEALAHPTWSMGPKITVDSSTLVNK
                     GLEVIEAHELFGIDYDHIDVVVHPSSIVHSMATFSDGSTIAQLSNPTMHLPIGYALAW
                     PDRLPTAFGEIDWTQPLELRFDPPDLEAFPCLGLAFDAGRAGGTAPAWFSAANEVVVD
                     AFLAGRVRWVDIAALNAAALDAWDGLAADSVEAVLHADREARRHAESLLLNAEVPS"
     gene            1313..>2062
                     /locus_tag="PLP95_12070"
     CDS             1313..>2062
                     /locus_tag="PLP95_12070"
                     /inference="COORDINATES: protein motif:HMM:NF014244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HPB46583.1"
                     /translation="MTTPIDHTDNAGTEPAGTEPAGTEPVTAAAAAAAVDPDDLGRSG
                     VRGERAEIGKPYVRWSRLSILVALLAAVGIWQGWRTLLLIGAVIVMIFFHELGHFVMA
                     RRADMKVTEFMIGFGPRIASFRRGDVEYGLKAIPAGAYVKIIGMANIEEVAPGDEDRT
                     YRQKGYWQRMGVAVAGSAMHFIMALLLIGSSLVFIGLGDPSNWMVDNISPGSAAERAG
                     VQLGDKVVEFGGVSVDSWAAMSAEARANPDRR"
BASE COUNT          334 a          658 c          693 g          377 t
ORIGIN      
        1 gttctccgat aaccagtggt cgtgatcacg accactggtt atcggagaac gggtccgggg
       61 tggtgggtgg cgggcgcggg ctgacacggt gctcaggagc cagcggccag agtgtccgta
      121 ctcggcgata cgatgcgggg atcatgacga cgattgcagt gatggggtcg accggttcga
      181 tcgggaccca gacccttgag gtcatcgcaa ccgatccgca gcggtttcag gtcgattcgc
      241 tcggggcgtt cggcggcaat ctcgatctct tggccaaaca ggccaacacg gttcgtccac
      301 gcgtcgtcgc tcttgctgac gacgccaagg ccgcggaact caaggagcga ctcgatcccg
      361 ggatcgaact ggtcgccggc ccggattcgc ttgcggaggt gtccacggcg gccgatgtca
      421 cgatcaacgg ggtggtcgga ttcgccggtc tcggcgtgac aatggaggtg ctgcgctccg
      481 gcaagcgcct cggactcgcc aacaaggagt cgttgatcgc cgcaggcccg gtcgtgcaac
      541 cgctgcgtgc aaccccgggc gccgaactga tcccggtcga ctccgagcat tgcgcgatcc
      601 accaatgtct tcgctccgga gcacaaggtg ctggcggccc tcgcctcaag cgaatcgtgc
      661 tcaccgcctc gggcgggccg tttcgagggc gatcgcttcg cgaactcgag tcggtgacgg
      721 tgcaagaggc gctcgcgcat ccgacctggt cgatgggccc gaagatcacc gtcgactcct
      781 ccacgctggt gaacaagggc cttgaggtga tcgaggccca cgaactgttc ggcatcgact
      841 acgaccacat cgacgtcgtg gtgcatccga gttcgatcgt ccactcgatg gcgacgttca
      901 gcgacggttc aacgatcgcc cagctgtcga acccgacgat gcacctgccg atcggatatg
      961 cgctggcctg gcccgatcgg ctcccgaccg ccttcggcga aatcgactgg acacagccgc
     1021 tcgagctgcg tttcgacccg cccgacctcg aggcgttccc gtgccttggt cttgccttcg
     1081 acgccggacg cgccggcggc accgctccag cctggttctc cgcggcgaac gaggtggtgg
     1141 tcgacgcgtt cctggcgggg cgagtccgct gggtcgacat cgcggcgctc aacgcggccg
     1201 cactcgatgc ctgggacggg ctcgcggccg acagcgtcga agccgttctg catgcggacc
     1261 gcgaggcccg ccgtcacgcc gagtcattgc ttctcaacgc cgaggttccc tcatgacgac
     1321 tccgatcgac cacacggata atgcgggcac cgaaccggct ggcaccgaac cggctggcac
     1381 cgaaccggtt actgccgcgg ccgctgccgc agcggtcgat cccgacgatc tcggccgcag
     1441 cggtgtgcgc ggcgaacgcg ccgaaattgg caagccgtac gtgcgttgga gccggctgtc
     1501 gattctcgtc gccctgttgg cagcggtggg catctggcag ggctggcgca cgctgctgct
     1561 catcggcgcc gtcatcgtga tgatcttctt ccatgagctc ggccacttcg tgatggcgcg
     1621 gcgtgctgac atgaaggtca ccgagttcat gatcgggttc ggtcctcgca tcgcttcgtt
     1681 tcgccgcggt gatgtcgaat acgggctcaa agccattcct gccggggcgt atgtgaagat
     1741 catcggaatg gccaacatcg aggaggtcgc tcccggtgac gaggaccgaa cctaccgcca
     1801 gaaggggtat tggcagcgca tgggcgtggc ggtcgctggt tcggcgatgc acttcatcat
     1861 ggccctgttg ctgatcggat cgtcgctcgt gttcatcggc ctcggcgatc cgtcgaactg
     1921 gatggtcgac aacatctctc cggggagcgc ggctgaacgt gccggggtgc agcttggcga
     1981 caaggtggtc gagttcggag gcgtgagcgt cgattcttgg gctgccatgt cggcagaggc
     2041 acgagccaac cccgaccgcc ga
//
LOCUS       DAOOMA010000526         2059 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_68414_length_2059_cov_3.437126, whole genome shotgun sequence.
ACCESSION   DAOOMA010000526 DAOOMA010000000
VERSION     DAOOMA010000526.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 2059)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2059)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2059
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68414_length_2059_cov_3.437126"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1240
                     /locus_tag="PLP95_12075"
     CDS             <1..1240
                     /locus_tag="PLP95_12075"
                     /inference="COORDINATES: protein motif:HMM:NF024850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HPB46584.1"
                     /translation="GNMRRFASALLVVAALLATSCAADDDNQAVSTDTTVATGDTDSP
                     TGDTVKFGTLDSPCGEAPDGKVATVKAEDAGRGTDKLYIGVANDRASIRAGLLQELWE
                     TTTAFVSWCNDQGGINGLPLEAIDLDGKLFEIEGAMATACTDTFALVGGGWAQDNLMF
                     TGKDGSDFHKCGLIAFPGFAASPEFAGATDIVNVGPNPPNRQPTASLDALAQAFPGET
                     SNLAILFPDLDSLRTRAAQTIATAEQVDGFDQFHQIAYSPVSQDWGVLAQQVLASGAT
                     SVFAIGEPATLSLLNQALRDQNFEGPIATEINVYDQRTIDSAGPEAAEGITVAMRSYF
                     GPFEEADEYPVTQQLIDILDEYSPNWTRASLVANSWSANLLFATTAKACAQEGEIRGS
                     ACCKRARRWTTGTPEGSVSP"
     gene            1237..1428
                     /locus_tag="PLP95_12080"
     CDS             1237..1428
                     /locus_tag="PLP95_12080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB46585.1"
                     /translation="MNIAEDTASLCSILMQVRDGKFQRLYPEKGGADAENMGMHCGEL
                     VDIEGDFGAGNRESSILDN"
     gene            1505..>2059
                     /locus_tag="PLP95_12085"
     CDS             1505..>2059
                     /locus_tag="PLP95_12085"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HPB46586.1"
                     /translation="MTAPRAAPAFRSDFEPRYGEVERVAPGIRRILAHNPSKYSAWGT
                     GTYFIGGDLAGGGPGGPVAIIDPGPDLDSHHRALDAALEGEEVAAILVTHTHADHSPA
                     SARLAELTGAIRYGYGPHPAAAIPAARDEAEPDEDGADEESGSYDAEFVPDVTLRDGD
                     VIGADLHPGADWRFVAIHTPGHISN"
BASE COUNT          375 a          712 c          644 g          328 t
ORIGIN      
        1 ggggaatatg cgccgtttcg cttcggctct cttggtcgtc gccgcgctgc tcgcgacatc
       61 gtgcgctgcc gacgacgaca atcaggccgt cagtaccgac acgaccgtcg caaccggcga
      121 caccgacagc ccgaccggcg acaccgtcaa gttcggcacg ctcgacagcc cgtgtggtga
      181 agcacccgac ggcaaggtcg caaccgtgaa ggccgaagat gccggccgag gaacggacaa
      241 gttgtacatc ggcgtcgcca acgaccgggc ctcgatccgt gccgggctgc ttcaggagtt
      301 gtgggagacc acgaccgcgt tcgtgagctg gtgcaacgat cagggcggca tcaacggcct
      361 tccgctcgaa gccatcgacc tcgacggcaa gctcttcgag atcgaaggag cgatggccac
      421 ggcctgcacc gacaccttcg ccctcgtcgg cggcggttgg gcccaagaca acctgatgtt
      481 caccggcaag gacggctccg atttccacaa gtgcggcttg atcgccttcc ccggattcgc
      541 cgcatcgccg gaattcgccg gagcgaccga catcgtcaac gtcggcccca acccgccgaa
      601 tcgccaaccg acggcctcgc tcgacgccct cgcccaggcg tttccgggcg agacatcgaa
      661 tctggccatc ctgttccccg atctcgattc tcttcgtacc cgagcggccc agaccatcgc
      721 gaccgccgag caggtcgacg gcttcgacca gttccaccag atcgcctaca gcccggtgag
      781 ccaggactgg ggggtgctcg cccagcaggt tcttgcaagc ggcgcgacct cggtcttcgc
      841 aatcggcgaa ccggccaccc tttcgttgtt gaatcaagcg cttcgcgatc agaacttcga
      901 aggtccgatc gcgacggaga tcaacgtcta tgaccagcgc acgatcgact ccgcaggacc
      961 cgaggccgcc gaaggcatca ccgtggcgat gcgcagctac ttcggtccgt tcgaggaggc
     1021 cgacgagtat ccggtcaccc agcaactcat cgacatcttg gatgagtact cgccgaactg
     1081 gacccgcgcc tcgctcgtcg ccaactcgtg gtcggccaac ctgttgttcg ccacgacggc
     1141 caaggcctgc gcgcaagagg gtgagatcag ggggtctgca tgctgcaaaa gggccaggcg
     1201 gtggacgact gggacaccgg agggctcggt ctccccgtga atatcgccga ggacaccgca
     1261 tcgttgtgtt cgatcctgat gcaggtgcgc gacggcaagt tccagcggct ctaccccgaa
     1321 aagggcggtg ccgatgccga gaacatgggc atgcactgcg gcgaactcgt cgatatcgaa
     1381 ggcgacttcg gtgccggcaa ccgggagtcg tcgattctcg acaattagcg gcgggttcga
     1441 taccccggcc accgctctcg gtggccgggg tattggccgg gggatcggcc ggtaggattc
     1501 gcccgtgact gctcctcgtg cggcaccggc gttccggagc gattttgagc ctcgctacgg
     1561 cgaggtcgaa cgggtcgccc ccggcattcg acgaatcctg gcgcacaacc cgtcgaagta
     1621 cagcgcctgg ggcacgggca cctacttcat cggcggcgac ctcgccgggg gcggaccggg
     1681 cggacccgtc gccatcatcg accccggacc tgacctcgac agtcaccacc gtgcactcga
     1741 cgcggcgctc gagggcgagg aagtcgccgc catcctcgtc acccacaccc acgccgatca
     1801 ctcccccgcg tcggctcgcc tcgcagaact caccggggcg attcgctacg gctacggacc
     1861 acatccagcc gccgcgattc cggccgcacg cgacgaagcc gagccggatg aggacggcgc
     1921 tgatgaagag tctggctcct acgatgccga gttcgtccca gacgtgacac tgcgcgacgg
     1981 cgacgtgatc ggagccgacc tgcatccggg ggccgactgg cgctttgtgg cgatccacac
     2041 gcccggacac atctcgaat
//
LOCUS       DAOOMA010000527         2059 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_68426_length_2059_cov_2.513473, whole genome shotgun sequence.
ACCESSION   DAOOMA010000527 DAOOMA010000000
VERSION     DAOOMA010000527.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 2059)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2059)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2059
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68426_length_2059_cov_2.513473"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1418
                     /locus_tag="PLP95_12090"
     CDS             <1..1418
                     /locus_tag="PLP95_12090"
                     /inference="COORDINATES: protein motif:HMM:NF040138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HPB46587.1"
                     /translation="SLLATSSMSTARGNQTSRLSIALTGMGEVVKSLTYVRCADTVEY
                     QDAFDKNENAAKLSSQQIDQGGTALTFTVVDVTGCESGVDQGTQDVTIRVSSGGSSLE
                     ATVVKRDPEPLSRDMFVVLVGTPISTAGDVLYGFQLSGEGSFSPRDIVEYRFECAADP
                     PTTSTENPDVDGPTTIVTSDPNDPAARCVYRARSDSFDAVVTLTITDSSGVMVSATER
                     WLVPPASSVPLAPTVTFEWTPKTITQATKTFTSTSPTPVVSPIVRWEWDFGDGSPRVS
                     CTDGSCATTSHTYTSSGTYTAELRLTDSLGLIGVGASDPIVISIASPTSTTPAPTTTI
                     APQTISISKNVYKTAGCYSGAAGEDTFLEQLGTDGWWHFYFQGCVGSESVALHDPPGG
                     PFADAFHAPTGKKCQMTLERTNGSIIATTKWYGPNTPTPAPIADIGPVADTGGWVFIL
                     GGAFKSWPSTMVWRITCTDVP"
     gene            1370..>2059
                     /locus_tag="PLP95_12095"
     CDS             1370..>2059
                     /locus_tag="PLP95_12095"
                     /inference="COORDINATES: protein motif:HMM:NF019575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HPB46588.1"
                     /translation="MAIDDGVEDHMHRRSLTASPHRRNDRRRNEHGYTLIEVMIASLL
                     AGILLAGLAGWTMTSMRSLSQVRGWAIDQAGTDRINRTLVNDAATALVIVTPTTGSPM
                     LDCQNLPLPNVYYGEPDLTPDVVALVGSLNFTLSNWARIIKWYANPRQYVTGVSSGAT
                     LDVAADKTLMLPTGATLGLLQINSDLAGTAEFARRLEEAVHELTATPAIATGKLESVG
                     ALSGVALQVLYA"
BASE COUNT          351 a          670 c          686 g          352 t
ORIGIN      
        1 cctcactgct ggcgacgagt tcgatgtcga cggctcgtgg caaccagacg agtcggctca
       61 gcatcgcgtt gacgggtatg ggcgaggtgg tcaagagcct cacctacgtg cggtgcgcgg
      121 acacggtcga ataccaggac gcgttcgaca aaaacgagaa cgcggcgaaa ctcagctcac
      181 aacagatcga tcagggcggc actgcgctga cgttcacggt cgtcgatgtc accggctgtg
      241 agtccggggt ggatcagggc acccaggacg tgacgattcg cgtcagttcc ggcggcagtt
      301 cgctggaagc gaccgtggtg aagcgcgatc cggaaccgtt gtcgcgcgac atgttcgtgg
      361 tgttggtcgg cactccgatc agcaccgccg gcgatgtgtt gtacgggttc cagttgtcgg
      421 gcgaggggtc gttttcgccg cgggacatcg tcgagtatcg gttcgagtgc gccgccgacc
      481 cgccgaccac ctccaccgag aaccccgacg tcgacggccc gaccaccatc gtgacctccg
      541 accccaacga ccccgccgca cggtgtgtgt atcgggcacg ttcggactcc ttcgatgcgg
      601 tggtgacgct gacgatcacc gattcgtcgg gggtcatggt gtcggcaacc gagcggtggc
      661 tggtgcctcc ggcgagttcg gtgccgctcg ctccgacggt gaccttcgag tggacgccca
      721 agacgatcac ccaagccacc aagacgttca cgtcgacgag tcccaccccg gtggtgtcgc
      781 cgatcgtgcg gtgggagtgg gatttcggcg acggctcgcc gcgggtgtcg tgcaccgacg
      841 gcagttgtgc caccacctca cacacctaca cctcctcggg cacttacacc gcggagctcc
      901 gcctgaccga cagcctcggg ttgatcgggg ttggcgcctc ggatccgatc gtcatttcca
      961 tcgcttcccc gacgagcacc actccggcgc ccacgaccac gatcgccccg cagaccatct
     1021 cgatttcgaa gaacgtctac aagaccgccg gttgctacag cggcgccgct ggcgaggaca
     1081 cgttcctgga acaactcggc accgacggct ggtggcactt ctatttccag gggtgcgtcg
     1141 gcagcgagtc ggtggcgctt catgaccctc cgggcgggcc gttcgccgac gcgttccacg
     1201 ccccgaccgg caagaagtgc cagatgaccc tcgagcgtac gaacgggtcg atcatcgcca
     1261 cgacgaagtg gtacgggccg aacacgccga cccccgctcc gattgccgac atcggcccgg
     1321 ttgccgacac cggcggctgg gtgttcatcc tgggcggagc gttcaagtcg tggccatcga
     1381 cgatggtgtg gaggatcaca tgcaccgacg ttccctgacc gcgtcgccgc accgccgtaa
     1441 cgatcggcgc cgcaacgagc acggatacac gctgatcgag gtgatgatcg catcgttgct
     1501 cgcgggaatt ctgttggcgg gcctggcggg ttggacgatg acgtcgatgc gttcgctcag
     1561 ccaggttcgc ggctgggcca tcgaccaggc gggcaccgat cggatcaacc gcacgctggt
     1621 gaacgatgct gcgaccgcgc tcgtcatcgt gacgccgacc acggggtcgc cgatgctcga
     1681 ttgccagaac ttgcccttgc cgaacgtcta ctacggcgag ccggacctga cgcccgacgt
     1741 ggtggcgctc gtcggctcgc tcaacttcac gctcagcaac tgggcgcgca tcatcaagtg
     1801 gtacgccaac ccgcgccagt acgtgacggg tgtctcgtcg ggcgcgacgc tggacgtggc
     1861 ggccgataag acgttgatgc tgccgacggg cgcgacgctg ggcctgctgc aaatcaatag
     1921 cgacctggcc gggacggcag agtttgcgcg gcgactagag gaagcggtgc acgagttgac
     1981 ggcgacgccg gccatcgcca cgggcaagct agagagcgtc ggcgcgctgt cgggcgtggc
     2041 gttgcaggtc ctgtacgcg
//
LOCUS       DAOOMA010000528         2052 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_68767_length_2052_cov_3.288433, whole genome shotgun sequence.
ACCESSION   DAOOMA010000528 DAOOMA010000000
VERSION     DAOOMA010000528.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 2052)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2052)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2052
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68767_length_2052_cov_3.288433"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1070)
                     /locus_tag="PLP95_12100"
     CDS             complement(<1..1070)
                     /locus_tag="PLP95_12100"
                     /EC_number="2.8.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024860.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA];
                     GO_function: GO:0050656 - 3'-phosphoadenosine
                     5'-phosphosulfate binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HPB46589.1"
                     /translation="MSAANDLNTPEPVRIDDYAAPRFPPEVAEAIEGAKALDDLLDYS
                     VEGVVAQARGELQAAGIAEPDAGPDDYLGRMQLLFNAFWEHPRLSHMGRISLHTMFVQ
                     MTRNRMLLEDLLARHPEIHDIEIDRPIVIAGLPRTGTTHLQQLLAADPHLRSLPYWES
                     LEPIPMPTDVARADGRDGRWDRTNAACEFMDLAMPYFKRMHEMSADHVHEEIQLLAVD
                     FSSMFFEPLAPIPRWRDDFLARDQTPHYEYMKTVLKAMSFLRGGSKRWMLKSPQHLEQ
                     FVALRNVFGDATVVVTHRDPVAVTVSMATMAAYAARMQMDPVDPVEIGDYWSDRVTRM
                     LGAAVRDRDVLAPSLRMTTFQST"
     gene            complement(1067..>2052)
                     /locus_tag="PLP95_12105"
     CDS             complement(1067..>2052)
                     /locus_tag="PLP95_12105"
                     /inference="COORDINATES: protein motif:HMM:NF018452.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1214 domain-containing protein"
                     /protein_id="HPB46590.1"
                     /translation="RWIFSITQVALDCFVWADVDAPRFVDIVGTTKKWGGDNADAFYQ
                     HAPIGPGRRYRVSGWRGDAAYMSLTVYAGPDDGHYSERIVGTVNDEQLAFDEEGRFSF
                     WVAPEPLDDGPGIITDAESVVALTRDYLADPVAGRRCTWRIECIDDAPPYEFTDDDLA
                     RRFRAALTWVRDQAAIVPVGDMDSVNAVADPYPVPTATFGWAAGDAAYAMGSFELAEG
                     EQLVIRGRSPKCRFWNLVLWNPLLHTYNYDRDRVTINGAQVRYEPDGSFEIVVAASDP
                     GHPNWVSTQGHRRGRIWLRWFLPEHTPEPLTTSVEPIGAGPVGAGSIGGGR"
BASE COUNT          338 a          653 c          701 g          360 t
ORIGIN      
        1 gtggattgaa acgtcgtcat cctgagcgat ggcgccaaca cgtcgcggtc gcgcacggcg
       61 gcaccgagca ttcgggtgac tcggtcggac cagtagtcgc cgatctcgac ggggtcgacg
      121 ggatccatct gcatccgggc ggcgtacgcg gccatggtcg ccatcgacac ggtcacggcc
      181 accggatcgc ggtgagtcac cacaacggtg gcgtcgccga acacgttgcg cagcgccacg
      241 aattgttcga ggtgctgggg cgatttcaac atccaccgtt tcgatccgcc gcgaaggaac
      301 gacatcgcct tcaacaccgt cttcatgtac tcataatgcg gcgtctgatc gcgggcgagg
      361 aagtcgtcgc gccaccgcgg gatgggtgcg agcggttcga agaacatcga gctgaagtcg
      421 acggcgagga gctgaatctc ctcgtgcacg tggtcggcgc tcatttcgtg catacgtttg
      481 aaatacggca tggcgaggtc catgaactcg cacgcggcgt tggtgcgatc ccagcgccca
      541 tctcgtccgt cggcgcgggc gacgtcggtc ggcatcggga tcggctcgag cgactcccag
      601 tacggcaacg accgcaggtg gggatccgcg gcgagaagct gttgcagatg ggtcgtgccg
      661 gtgcggggta gcccggcgat gacgatgggc cggtcgatct cgatgtcgtg gatctcggga
      721 tggcgggcta gcaaatcttc cagcagcatc cggttacggg tcatctgcac gaacatggtg
      781 tgcagcgaga tgcgccccat gtggctgagt cgcggatgct cccagaaggc gttgaagagc
      841 agttgcatcc tccccaggta gtcgtcgggg cccgcgtcgg gttcggcgat ccccgccgcc
      901 tgaagctccc ctctcgcctg ggcgaccacc ccctccacgc tgtagtcgag gaggtcgtcg
      961 agcgccttgg ctccctcgat cgcctcggcg acctcgggcg gaaaccgcgg tgctgcgtaa
     1021 tcgtcgatgc gcacgggctc gggcgtattc aggtcgttgg cagcgctcat cgcccgcctc
     1081 cgatcgaccc ggctccgacc ggcccggctc cgatcggttc gaccgaggtc gtcaacggtt
     1141 cgggtgtgtg ttcgggcagg aaccaccgaa gccagatcct ccccctccga tgcccctggg
     1201 tcgacaccca gttcgggtga cctggatcgc tcgcggcgac cacgatttcg aagctgccat
     1261 ccggttcgta gcgcacctgc gcaccgttga tcgtgacgcg atcgcggtcg tagttgtagg
     1321 tgtgcaacag cgggttccac agcacgagat tccagaatcg gcacttcggg gaacgtcccc
     1381 ggatgacgag ttgctcgccc tcggccagct cgaaggatcc catggcgtag gcggcatccc
     1441 ccgcagccca tccaaaggtc gccgtgggaa ccgggtacgg gtcggcgacc gcgttcacgc
     1501 tgtccatatc gccgaccggc acaatcgccg cctggtctcg cacccaggtg agcgcagcgc
     1561 ggaaacggcg cgcgagatcg tcgtcggtga actcgtatgg tggcgcgtcg tcaatgcatt
     1621 cgatgcgcca ggtgcagcgg cggcctgcga ccgggtcggc caggtagtca cgggtgaggg
     1681 ccaccaccga ctcggcgtcg gtgatgatgc ccggcccgtc gtcgaggggt tcgggcgcca
     1741 cccagaacga aaagcggccc tcctcgtcga aggcgagctg ctcgtcgttg acggtgccga
     1801 cgatgcgttc ggagtagtga ccgtcgtcgg gtccggcata gacggtgagc gacatgtacg
     1861 ccgcgtcgcc gcgccagcca ctcacgcggt aacggcggcc gggcccgatc ggggcgtgtt
     1921 ggtagaaggc atcggcgttg tcgccgcccc acttctttgt ggttcccacg atgtcgacga
     1981 agcgtggggc gtcgacgtcg gcccagacga agcagtcgag cgccacctgg gtgatcgaga
     2041 agatccaccg gt
//
LOCUS       DAOOMA010000529         2043 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_69221_length_2043_cov_4.149396, whole genome shotgun sequence.
ACCESSION   DAOOMA010000529 DAOOMA010000000
VERSION     DAOOMA010000529.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 2043)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2043)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2043
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_69221_length_2043_cov_4.149396"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(421..1671)
                     /locus_tag="PLP95_12110"
     CDS             complement(421..1671)
                     /locus_tag="PLP95_12110"
                     /inference="COORDINATES: protein motif:HMM:NF012656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HPB46591.1"
                     /translation="MDLTYPPEAETFRVEIRTWLEENLPQGWFDEGFEMTPEEKRRFN
                     DEWTTKLFDGGWICATWPTEYGGKGLTTIQGVVLSEEFARAKAPMRADFFGDTLVGPT
                     ILMNGTEEQKKYFLPRILNGTMRWCQGFSEPNSGSDLASLKTSAVLDGDEWVINGQKV
                     WTTQAQFADYCFVLARTDPDAPKIQAGISYLLVPMDQPGIEVRPIIQPDGTAEFNEVF
                     FENARCPKDNVVGELNNGWKVANDTLTFERGMSATTGHRRFEEEHRRMVAAAKANGKI
                     DDPFVRQGLAKYYSKIQILRINGLRNLTAALTGKRDAGTAALGATNKLFWSEMHQDAM
                     QLALAIFGAGSMLIDAGPEDGSWPGQGRLEKRDHYPVSPMMSTYFFSRSETIWGGSSQ
                     IQRNIVGERVLGLPREPKPAAPKN"
     gene            1809..>2043
                     /locus_tag="PLP95_12115"
     CDS             1809..>2043
                     /locus_tag="PLP95_12115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HPB46592.1"
                     /translation="MSIVIETPPAPAKKERWTNQWLELKAEVIDTGLCTGCAGCVIAC
                     PHDVIGYNHEAGGYRPFHLEEELGPDDCVHGQKG"
BASE COUNT          354 a          687 c          643 g          359 t
ORIGIN      
        1 ggcggcaccg gcgagcagca ccatcgcaca accgacgacc agcgcccagc gaccgaggag
       61 ccgggcccgc gtcgggcggc cgggtggcgg ttgcgacggg cgtgactgcg aggctcgctg
      121 ctgcggcgcc cggggctgcg gcgcacgctg ctgcggcgcc cggggctgcg acccacgctg
      181 ttgcgacgag gattgccgac cgtcgccgcg aaccatgcgc gcggcacccg acggcggacg
      241 ctgaccgccc gaggaactcg ctgaattgga tggacggcgc gacgatcggt tgtcgccccc
      301 acctcggggt gtctgcggca cggcgtcgag cgtaatcgtt tcgcaacacg gcccccggtc
      361 acccaccgaa cctaagggcc acatgggtcc gggttgcccg acaacggttg cgccagagcg
      421 ctaattcttc ggtgcagccg gcttcggttc gcgaggaagg cccagcaccc gttcacccac
      481 aatgttgcgc tggatctgac tcgatccgcc ccagatcgtt tcggaacggc tgaagaaata
      541 ggtgctcatc atcggcgaca ccggatagtg atcgcgcttt tcgagtcgtc cctgccccgg
      601 ccaggagccg tcctcgggac cggcgtcgat cagcatcgat ccggccccga aaatcgcgag
      661 cgccagttgc attgcatctt ggtgcatctc cgaccagaac aacttgttcg tcgccccgag
      721 cgcggcggtg cccgcatcgc gcttgccggt gagcgcggcg gtcaggttcc gcagaccgtt
      781 gatccgcaga atctggatct tcgagtagta cttcgcgagc ccctgacgaa cgaacgggtc
      841 gtcgatcttg ccgttcgctt tggcggccgc caccatccgg cggtgctcct cctcgaaacg
      901 gcggtgcccg gtcgtcgccg acatgccgcg ttcaaaggtc agcgtgtcgt tggcgacctt
      961 ccagccgttg ttgagttcgc cgacgacatt gtccttcgga caacgggcgt tttcgaagaa
     1021 cacctcgttg aactcggccg tgccgtcggg ctggatgatc gggcggacct cgatgccggg
     1081 ctgatccatc ggcaccagca gatacgagat tcccgcctgg atcttcggag cgtcggggtc
     1141 ggtgcgggcc aacacgaagc agtagtcggc gaattgcgcc tgggtggtcc acaccttctg
     1201 gccgttgatc acccattcgt caccgtcgag caccgcggag gtcttgagcg acgcgaggtc
     1261 cgagcccgag ttcggctccg agaagccctg acaccatcgc atcgtgccgt tgaggatgcg
     1321 cggcaggaag tacttctttt gttcctcggt gccgttcatg agaatcgtcg gaccaaccag
     1381 cgtgtcgccg aagaagtcgg cccgcatggg agccttcgcc cgagcgaact cctcgctcag
     1441 caccacgccc tggatggtcg tgagcccctt acccccgtac tcggtcggcc acgtcgcaca
     1501 gatccaccca ccgtcgaaca gcttcgtggt ccattcgtcg ttgaaccggc gcttttcctc
     1561 gggggtcatt tcgaacccct cgtcgaacca accctgggga aggttctctt ccaaccaggt
     1621 gcgaatctcg acgcggaacg tttcggcttc gggtgggtag gtcagatcca tgtcgacatt
     1681 cttgaccgac cggtcagctt tcgccacccg acccgttggc gcgacctccg cgccgcaccg
     1741 gcgcccgtca gctgacagag acgaaaagga tgggacgatc caggcgtcac acaactaagg
     1801 tcaacaccat gtcaattgtg atcgagaccc ctccggctcc ggccaagaag gaacgctgga
     1861 ccaaccagtg gctcgaactc aaagccgagg tcatcgacac cggtctgtgc acgggatgcg
     1921 ccggatgcgt catcgcatgc ccccacgacg tcatcgggta caaccacgag gccggcggct
     1981 accgcccgtt ccacctcgag gaggaactcg ggccggacga ctgcgtgcac ggccagaagg
     2041 gtt
//
LOCUS       DAOOMA010000530         2043 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_69226_length_2043_cov_3.679577, whole genome shotgun sequence.
ACCESSION   DAOOMA010000530 DAOOMA010000000
VERSION     DAOOMA010000530.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 2043)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2043)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2043
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_69226_length_2043_cov_3.679577"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..401
                     /locus_tag="PLP95_12120"
     CDS             <1..401
                     /locus_tag="PLP95_12120"
                     /EC_number="4.1.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012223190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004611 - phosphoenolpyruvate
                     carboxykinase activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase
                     domain-containing protein"
                     /protein_id="HPB46593.1"
                     /translation="GRGQDDQRQKEGADAEKLPKMFWVNWFRKDSDGKFMWPGYGDNS
                     RVLKWVLERLDGSADATETAIGNVPTVDAIDRTGLDIDDEMMAKILEVDNDAWRQEIP
                     LVEGHFAFIGERLPTEMRDELADLEKRLAN"
     gene            complement(555..917)
                     /locus_tag="PLP95_12125"
     CDS             complement(555..917)
                     /locus_tag="PLP95_12125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB46594.1"
                     /translation="MSVQDLHGDLAWAVVVANGLVGSWALGAHWLAALRRRALWWFVV
                     ASQVLIFPQMAFGVWLMAVDGVEPPSMHTFYGFLLPFTVLILFGYSRTSVRSYLYLLY
                     GLGGWFLMGLGIRAMILH"
     gene            960..1277
                     /locus_tag="PLP95_12130"
     CDS             960..1277
                     /locus_tag="PLP95_12130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB46595.1"
                     /translation="MRHAQFHIADIQLGTRSLPLHYGTLQVVDREGTIDWELVLHTID
                     PEPVAQAIHLLAFRSITGVEESGALTLTPMHGEAALVRWVDNSLVFRGAGLLDGFNES
                     QFG"
     gene            complement(1327..>2043)
                     /locus_tag="PLP95_12135"
     CDS             complement(1327..>2043)
                     /locus_tag="PLP95_12135"
                     /inference="COORDINATES: protein motif:HMM:NF014625.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HPB46596.1"
                     /translation="HGEVVPGVVVSGGQLGIHRVAETFASAEVLGVQRIEFLGYIDSG
                     MIGTPENDAPYSFWGADVDDAASRLAAILRDEEADVLVAYDPNGNYGHPDHIQVHRVG
                     YRAAELAGTPRVYEMTINRDRMIEAFLARRDTATAEELEQLPEIDPDSFQMGMPDAEI
                     THSIDVQALTATKRAAMRCHASQIGEDHFFLTLSDDDFALAFGTEWLSTGPGPGAQAR
                     RISPICSPEPGRRSDCPFCR"
BASE COUNT          395 a          676 c          627 g          345 t
ORIGIN      
        1 caggccgagg gcaggacgat cagcgccaga aagagggcgc tgatgccgag aagcttccga
       61 agatgttctg ggtcaactgg ttccgcaagg actccgacgg caagttcatg tggccgggct
      121 acggcgacaa cagccgtgtc ctcaagtggg tgctcgaacg cctcgatggt tccgccgacg
      181 cgaccgagac cgctatcggc aacgtgccga ccgtcgacgc gatcgaccgg accggcctcg
      241 acatcgacga cgagatgatg gcgaagatcc tcgaggtcga caacgacgcg tggcgccagg
      301 agatcccact cgtcgagggc catttcgcat tcatcggaga gcgcctgccg acagagatgc
      361 gcgacgaact cgccgacctc gaaaagcgtc tcgccaacta ggcacctgcc tgtacctgcc
      421 tgtacctgcc cggcggttgc cggctcctgc ctgttctccg ataaccagtg gtcgtgatca
      481 cgaccactgg ttatcggaga acggcatttt tggggcggtt ttggggcggt ttttggggcg
      541 gttctggggc cggatcagtg caggatcatc gcccggatcc ccaagcccat gaggaaccat
      601 ccgccgagtc cgtacagcag gtacagatac gaacgcaccg aggtccggct gtatccgaac
      661 aggatcaaca cggtgaaggg cagcaggaac ccgtagaacg tgtgcatgct cggcggctcc
      721 accccgtcga ccgccatcag ccagacgccg aaagccatct gcgggaagat caagacctgc
      781 gacgcgacga cgaaccacca caacgcccgc cgacgcagcg cggcaagcca gtgcgcaccg
      841 agcgcccacg acccgaccaa cccattggcc accacgaccg cccacgccag gtccccatgc
      901 aggtcttgaa cgctcaccgt gtcagcctac cgacgacacc aacgtaggct tggccgacga
      961 tgcgacacgc gcagttccac atcgcggata tccaactcgg cacccgctcg ctccccctgc
     1021 attatggcac cctccaggtc gtcgatcgcg agggaacgat cgactgggaa ctcgtcttgc
     1081 acaccatcga ccccgaaccg gtcgcccagg cgattcatct tctggcgttt cgctcgatca
     1141 ccggggtcga ggaatccggt gcactcaccc tcactccaat gcacggtgag gcggcgctcg
     1201 tgcgttgggt cgacaacagc ctggtgtttc gaggagccgg tctgctcgac ggattcaacg
     1261 aatcgcagtt cggctaaccg agcgaccggg ttctgtaggt acattctgcg gccatagcca
     1321 ccgcgattac ctacagaacg ggcaatcgga gcggcgaccg ggttcaggtg aacagatcgg
     1381 tgagatacgc ctcgcctgcg ccccggggcc gggaccggtc gataaccact cggtgccgaa
     1441 cgccagtgcg aaatcgtcat ccgacagcgt caggaagaag tggtcctcgc cgatctggct
     1501 ggcgtggcaa cgcattgccg cccgctttgt cgccgtcagt gcctgcacat cgatcgaatg
     1561 ggtgatctcc gcatcgggca ttcccatctg gaaactgtcg gggtcgatct cggggagttg
     1621 ttcgagttcc tcggccgtgg cggtgtcgcg tcgggcgagg aacgcctcga tcatccggtc
     1681 tcggttgatg gtcatctcat agacacgtgg cgttccggcc aactccgccg cccgatagcc
     1741 cacccggtgc acctggatgt gatccgggtg accgtagttt ccgttggggt cataggccac
     1801 cagaacgtcc gcctcctcgt cgcgcaggat cgcggccaga cgcgatgccg cgtcatcgac
     1861 gtcggctccc caaaagctgt acggggcgtc gttttcgggg gtcccgatca tgcccgaatc
     1921 gatgtagccg agaaactcga tccgctgcac gccgagcacc tcggccgacg cgaacgtctc
     1981 ggcgacccgg tggatgccga gttgcccgcc ggagaccacg acacccggga cgacctcgcc
     2041 gtg
//
LOCUS       DAOOMA010000531         2036 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_69541_length_2036_cov_3.431095, whole genome shotgun sequence.
ACCESSION   DAOOMA010000531 DAOOMA010000000
VERSION     DAOOMA010000531.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 2036)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2036)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2036
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_69541_length_2036_cov_3.431095"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..454)
                     /locus_tag="PLP95_12140"
     CDS             complement(<1..454)
                     /locus_tag="PLP95_12140"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HPB46597.1"
                     /translation="MTKISRRPDRATPLVRRLDGFTSTVFAEMSLLAAETGSFNLGQG
                     FPDSDTPAAVVEAATRAMCDGVNQYTHPLGEPVLRQAIADHQQRFWDMTVDPQREVLV
                     TAGATEAIAATVLSLIEPDEEVLMFEPYFDSYAAVVALAGARRRVVTLR"
     gene            528..>2036
                     /locus_tag="PLP95_12145"
     CDS             528..>2036
                     /locus_tag="PLP95_12145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012230675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HPB46598.1"
                     /translation="MSNGWNLAELWERIAARFPDSPAHIQGDRTILWSETDERANGVA
                     HTLLEAGAQHQDKVALYLYNAPEYLEGSFAAYKAGLVPVNTNYRYVDDELLYLWDNAD
                     AVAVIFHGEFTPRIQGIRDRLPKVKTWLWVDDSTGPKPDWAIAYEDAAAAHPANTTAP
                     WGRSGDDLVMIYTGGTTGMPKGVMWRQDDLMMATVSVTLPKLTEGYGNITYDELLSEV
                     VEPGGPGLPACPLMHGTGWFMGNLFLSNGGCCVTTVGRKFDPVELLDTVERNKVQSLV
                     MVGDAFGRPIVAALDENPGRWDISSLVLISSSGVMWSEPNKEALLRHNPGMLLVDVFS
                     SSEAIGLGQSASFAGAEAETAKFVLGPNARVIAEDGSDVVPGSGQRGRVAVGGATPLG
                     YYKDPEKSAGTFVMIDGRRFSIPGDWATVETDGTITLLGRGSVCINTAGEKVFPEEVE
                     EVLKLNESILDAVVVGVPDDKFGQAITAVVEPASGATVDEASVIAEVKEHLAG"
BASE COUNT          370 a          700 c          633 g          333 t
ORIGIN      
        1 cacggagggt caccacccgc cgcctcgctc cggccaatgc gacgacggcg gcataggagt
       61 cgaagtacgg ctcgaacatc agcacctcct cgtcgggttc gatcagggac agcaccgtcg
      121 ccgcgatggc ctcggtcgcc cccgcggtga ccaggacctc gcgctgtggg tccaccgtca
      181 tgtcccagaa gcgttgctga tggtcggcga tcgcctgacg caacacgggc tcgccgagcg
      241 gatgggtgta ctggttgaca ccgtcacaca ttgcccgggt tgccgcctcg accacggccg
      301 ccggggtgtc ggagtccggg aagccctgac cgaggttgaa cgacccggtc tccgccgcca
      361 gcagcgacat ttcggcaaac accgtggagg tgaacccgtc gaggcgccgt accagcggag
      421 ttgcgcggtc gggtcgccgt gaaatcttcg tcaccaacgc agcgtaggtg ctcccccaac
      481 tccggcggtg ctcctatgct gagcgccgac caaagggggc actacacatg tcgaacggat
      541 ggaatctcgc ggaactctgg gagcggatcg ccgctcgctt tcccgactca cctgcccaca
      601 ttcaggggga tcgcacgatc ctgtggagcg aaaccgacga gcgggccaac ggcgtcgccc
      661 acacgctgct cgaagccggt gcccaacacc aggacaaggt cgcgctgtac ctctacaacg
      721 cccccgaata tctcgaaggc tccttcgcgg cctacaaggc cggcctcgtt ccggtgaaca
      781 ccaactaccg ttacgtcgac gacgaactgc tgtatctgtg ggacaacgcc gatgccgtcg
      841 cggtcatctt ccacggcgag ttcaccccca gaatccaggg gatacgcgat cgtctcccca
      901 aggtgaagac ctggctgtgg gtcgacgact cgaccggccc caagcccgat tgggccatcg
      961 cctatgagga cgcggctgcc gctcatcccg ccaacaccac cgccccctgg gggcgcagcg
     1021 gtgacgacct cgtcatgatc tacaccggtg gcaccaccgg catgcccaaa ggcgtcatgt
     1081 ggcgtcagga cgacctgatg atggccaccg tttcggtcac gctgccgaag ctcactgaag
     1141 gatacggaaa catcacctac gacgaactcc tgagcgaggt cgtcgaaccg ggcgggcccg
     1201 gacttcccgc ctgcccgctc atgcacggca ccggctggtt catgggcaac ctgttcctgt
     1261 ccaacggcgg atgctgcgtc accaccgtcg gacgcaaatt cgatcccgtc gaactgctcg
     1321 acaccgtgga gcgcaacaag gtgcaatcgc tggtgatggt gggcgacgcg ttcggccgcc
     1381 cgatcgtcgc tgcgctcgac gagaaccccg gccggtggga catctccagt ttggtgctca
     1441 tctcctcgtc cggcgtgatg tggtccgagc ccaacaagga ggcattgttg cgccacaacc
     1501 cgggcatgct cctcgtggac gtgttctcct cctccgaggc catcggcctc ggacaatccg
     1561 ccagcttcgc cggagccgag gcggagacgg cgaagttcgt gctcggaccc aacgcccgcg
     1621 tcatcgccga ggacggctcc gacgtcgtgc ccggctcggg tcaacgcgga cgcgtcgctg
     1681 tgggtggggc gacccctctc ggctattaca aggaccccga gaagtcggcg ggaaccttcg
     1741 tgatgatcga tggccgccga ttctccatcc ccggcgattg ggccaccgtc gaaaccgacg
     1801 gcacgatcac cctgctcggc cggggatcgg tgtgtatcaa caccgccgga gaaaaggtgt
     1861 tccccgagga ggtggaagag gtgttgaagc tcaacgaatc catcctcgat gccgtcgtcg
     1921 tcggtgtgcc cgatgacaaa ttcgggcaag ccatcaccgc cgtcgtcgag ccggcctcgg
     1981 gcgccaccgt cgatgaggcc tccgtcatcg ccgaggtcaa ggaacacctc gccggg
//
LOCUS       DAOOMA010000532         2023 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_70191_length_2023_cov_4.509654, whole genome shotgun sequence.
ACCESSION   DAOOMA010000532 DAOOMA010000000
VERSION     DAOOMA010000532.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 2023)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2023)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2023
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_70191_length_2023_cov_4.509654"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>2023
                     /locus_tag="PLP95_12150"
     CDS             <1..>2023
                     /locus_tag="PLP95_12150"
                     /inference="COORDINATES: protein motif:HMM:NF023454.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3516 domain-containing protein"
                     /protein_id="HPB46599.1"
                     /translation="LSLYPAQEEATLSLFADEHAVITTPTGSGKSLVATAGHAEALAA
                     GGFSVYTAPIKALVSEKFFDFRAIFGADNVGMVTGDTQINPTAPILCCTAEILAHAVL
                     QSGRELPLRHVTMDEFHYFAERGRGWAWELPLLELHHTRFLLMSATLGDTGWLVEHLD
                     STTSRSAAVVGGGERPVPLSFRYAVTPIHETINELIGDDLAPVYVVYFTQKAASEAAQ
                     AFMSLNVLTKEEKQRAAEMMAGFRFDTPFGKDLRRHLAAGIGVHHAGLLPRYRLLVER
                     MAREGLLKLICGTDTLGVGVNIPIRSVLITQLCKYDGARTRILSVREFQQIAGRAGRR
                     GYDTSGTVVVQAPAYWIENRRDEAKAANAGKRLKHRKKAPEHGFKDWNSDTFDRLVGG
                     VPETLVSRFEVDGGAVLRVLDRPGDGVAQLRDIVSRSFAKESARDEMLTQIDDMLASL
                     VAADLVEELDEPDAEGRPYRPHPQLQERFSLDRPLSPFVVDMLTAIAEADADGSPGDD
                     VPSDDVPAESEQAVPATSPYAVALDMVSVVEASLEDPSAILQAQRRVLRDEEWAKLTA
                     EGVEFSERQDRVAEVDWPRPLRDELVAAWRIHLEANPWLAGEWVRPKSIARDMFEQGE
                     TLTGYVHRFGLRRSEGVLVRYLADCFRALERNVPDHLRTPELAEIGDWLA"
BASE COUNT          319 a          656 c          712 g          336 t
ORIGIN      
        1 ggctctcgct gtacccggcc caggaggagg ccaccctgtc gttgttcgcc gacgagcacg
       61 cggtgatcac gacgcccacg gggtcgggca agtcgctcgt cgccaccgcc ggccacgccg
      121 aggcactggc cgccggaggc ttctcggtct acacggcccc gatcaaggcg ctcgtgtcgg
      181 agaagttctt cgatttccgg gcgatcttcg gcgccgacaa cgtcgggatg gtcaccggcg
      241 atacgcagat caacccgacc gcgccgatcc tgtgttgcac cgccgagatc ctcgcgcacg
      301 ccgtcttgca gtcgggtcgc gagctgccgt tgcgtcacgt cacgatggac gagttccact
      361 acttcgccga acgggggcgg ggttgggcgt gggaactgcc gctgctcgaa ctgcaccaca
      421 cccgctttct gctgatgtcg gcgaccctcg gagacacggg ctggctcgtc gaacacctcg
      481 actcgacgac ctcgcgatcc gcggcggtcg tcgggggagg cgagcgcccg gtgccgctgt
      541 cgtttcgcta tgccgtcacg cccattcacg agacgatcaa cgaactcatt ggcgacgacc
      601 tcgctccggt ctacgtcgtg tacttcaccc agaaggcggc gagtgaggcc gcccaggcgt
      661 tcatgagcct caacgtgttg acaaaggagg agaagcagcg tgcggccgag atgatggcgg
      721 gcttccggtt cgacaccccc ttcggcaagg acctgcggcg acatctcgcc gcgggcatcg
      781 gcgtccatca cgcggggctg ttgccgcggt atcgactgct ggtcgagcgg atggcccgcg
      841 aggggctgtt gaagctcatc tgcggcaccg acaccctggg cgtgggggtg aacatcccga
      901 tccgatcggt gctcatcacg cagctgtgca aatacgacgg ggcgcgcacc cgcatcctca
      961 gcgtgcgcga gttccaacag attgccggcc gggccggacg acgtggctac gacacctccg
     1021 ggaccgtcgt cgtgcaggcg ccggcatact ggatcgagaa ccgtcgcgac gaggccaagg
     1081 cggcgaatgc gggaaagcgg ctcaagcacc gcaagaaggc gcccgagcat gggttcaagg
     1141 actggaactc cgacacgttc gatcggctcg tcggcggggt tcccgagacg ttggtctcac
     1201 ggttcgaggt ggatggcggc gcggtgctgc gggtgctcga ccgtcccggt gacggcgtgg
     1261 cgcaactgcg cgacatcgtg tcgcgcagct tcgccaagga atcggcccgc gacgagatgc
     1321 tcacccagat cgacgacatg ttggcctcgc tggtcgccgc ggatctggtg gaggaactcg
     1381 acgagcccga tgccgagggt cgtccgtacc ggccgcatcc acagctgcag gagcgctttt
     1441 cgctcgatcg accgctgtcg ccgttcgtcg tcgacatgct cacggcgatc gccgaggccg
     1501 acgccgatgg ctcgcctggc gatgatgtgc cgtccgacga tgtgcctgct gagagcgagc
     1561 aggcggtgcc cgcaacgtcc ccctacgcgg tggcgctcga catggtgagt gtggtcgagg
     1621 ccagtctcga ggacccctcc gcgatcctgc aggcgcagcg gcgggtgttg cgcgacgagg
     1681 aatgggcgaa gctcaccgcc gagggcgtcg agttctccga gcgccaggac cgtgtcgcag
     1741 aggtcgactg gccccggccg ctgcgcgacg agttggtggc ggcgtggcgg atccacctcg
     1801 aggccaaccc gtggctggcg ggggagtggg tgcggccgaa gtcgatcgcc cgcgacatgt
     1861 tcgagcaggg cgagacgctc accgggtacg tgcatcgctt cgggttgcgt cgttcggaag
     1921 gcgtgttggt ccggtacctc gcggattgct tccgggcgtt ggaacgaaac gttcctgacc
     1981 acctgcgcac accggagctc gcggagatcg gcgactggtt ggc
//
LOCUS       DAOOMA010000533         2019 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_70385_length_2019_cov_3.031059, whole genome shotgun sequence.
ACCESSION   DAOOMA010000533 DAOOMA010000000
VERSION     DAOOMA010000533.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 2019)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2019)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2019
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_70385_length_2019_cov_3.031059"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..507
                     /locus_tag="PLP95_12155"
     CDS             <1..507
                     /locus_tag="PLP95_12155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB46600.1"
                     /translation="TSPDGSTVFVVGGYDGKTGSTEVLRTTDGVNFTAIATLNHVVRY
                     PAVTVLDDTLWVVGGEYDNALSAAIQRIDLTTGAVTDLPELGTPVSRANAFTVGNSVF
                     VAGGRTAGGGSSAILRFEPYNGGFVVAGNLPTPRYDAGIVVEANRAWLIGGSTPQAST
                     DIVELTAS"
     gene            565..1254
                     /gene="pgl"
                     /locus_tag="PLP95_12160"
     CDS             565..1254
                     /gene="pgl"
                     /locus_tag="PLP95_12160"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: protein motif:HMM:NF013356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017057 - 6-phosphogluconolactonase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HPB46601.1"
                     /translation="MIQFRYIGTDEFAQVGAELLAERIDEAVAERGVCHLAVSGGRAP
                     WELFGVLGRRDLKWTQVHIWQVDERVAPDRDVDRNSVGLKTSLLALAPVPESNIHWMD
                     VTSDDLESAAAAYAEELDRVCGGVLDIVHLGLGADGNTASWSPGDPVSDVDDADVAIS
                     AEFNGFVRMTLTPRCVNRARGRIFLVTGGDKERAIDELTNGGDIPAAKVTPAATVAIV
                     ALPAPLHEDLD"
     gene            1427..>2019
                     /locus_tag="PLP95_12165"
     CDS             1427..>2019
                     /locus_tag="PLP95_12165"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HPB46602.1"
                     /translation="MAETSRRTQQDRSAATQRALLDATIECLVEYGYAGTTTRLVAER
                     AQVSRGAQTHHYPTKRDLVVAAVERLFDEQARHFTSAFDQVDPTVRTLEHAVEALWEI
                     ATGPAYAAVLEVLVAARTDEELRVVVHGVAAALETTVVDLLMWFAPAITDRDIARRLV
                     GLVFTLVQGGAVSRLGGFGQPDEVIRLATSMIDVALAA"
BASE COUNT          342 a          714 c          646 g          317 t
ORIGIN      
        1 acctcaccgg acgggtcgac cgtgttcgtg gtcggcggct atgacggcaa gaccggatcg
       61 accgaggtgc tccgcacgac cgatggcgtc aacttcactg ccatcgccac gttgaatcac
      121 gtcgtgcggt atccggcggt caccgtgctc gacgacacgc tgtgggtggt cggcggcgag
      181 tacgacaacg cgctgtccgc tgcgattcag cgcatcgacc tcaccactgg cgccgtcacc
      241 gacctgcccg aactcggcac gccggtgtct cgtgccaacg cgttcaccgt gggcaactcg
      301 gtgttcgtcg ccggcggccg aaccgccggc ggtgggtcat cggccatttt gcgtttcgag
      361 ccctacaacg gcggattcgt tgtcgccggc aacctgccga cgccacgcta cgacgccggg
      421 atcgtcgtcg aggccaaccg cgcctggctg atcggcggca gcaccccgca ggcgtcgacc
      481 gacatcgtcg aactcaccgc ctcctagcga tgtccctaca atgagtggtt cccgagtgtc
      541 gagccccctt cgaggccagc ggtcatgatc cagtttcgtt acatcggaac cgacgagttc
      601 gcccaggtcg gcgccgaact tctcgccgag cgcatcgacg aggcggtcgc cgaacgcggc
      661 gtgtgccacc tcgccgtttc cggtggtcgc gcaccgtggg agctgttcgg ggtcctcggc
      721 agacgagacc tgaagtggac ccaggtgcac atctggcagg tcgacgagcg ggtagctccc
      781 gaccgcgacg tcgatcgcaa cagtgtcggg ctcaagacct ccctgctcgc gctcgcacca
      841 gtgccggagt cgaacatcca ctggatggac gtgacctccg acgaccttga gtcagccgcc
      901 gcagcgtacg ccgaagaact cgaccgggtc tgtggcggcg tcctcgacat cgtgcacctc
      961 ggccttggcg cggatggcaa caccgcgagc tggtcgcccg gcgatccggt gagcgatgtc
     1021 gacgatgccg acgtggcgat cagcgccgaa ttcaacggat tcgtccgcat gaccctcacc
     1081 ccccgatgcg tgaatcgggc gcgggggcgc atctttctcg tcaccggtgg cgacaaggaa
     1141 cgggcgatcg acgagctcac caacggcggc gacatccccg ccgcgaaggt gaccccggcg
     1201 gccacggtcg cgatcgtcgc ccttccggca ccgctacacg aggatctcga ctgaacgagc
     1261 gcgcggcgcc gcgtcgaacg gtcctgctcg ggcggtcctg ctcgggcggc catgctcggg
     1321 cggtaatcca ctaccggtac ggacgcgagc gcggcgacgt cccattcgtg ataaacaaac
     1381 caacaggccg gttttaaaga cccccggcag gaacgacgag atcgccgtgg ccgagacctc
     1441 gagacgaaca caacaagacc gcagcgccgc aacccagcgc gccctgctcg acgcgacgat
     1501 cgagtgcctc gtcgaatacg gctatgcggg cacgaccacg cgcctggtcg ccgaacgagc
     1561 acaggtctcc cgcggtgcac aaacccacca ctacccgacc aaacgcgacc tcgtcgtcgc
     1621 cgcggtcgag cggctcttcg acgaacaggc gcgacatttc accagcgcgt tcgatcaggt
     1681 cgaccccacg gtgcggaccc ttgaacatgc ggtcgaggcg ttgtgggaga tcgcgaccgg
     1741 accggcctac gcggccgtcc tcgaagtgct cgtcgccgcc cgaaccgacg aggaattgcg
     1801 tgtcgtggtc cacggggtgg ccgccgcgct cgaaaccacc gtggtggacc tgttgatgtg
     1861 gttcgccccg gcgatcaccg accgcgacat cgcccgccgc ctcgtcgggc tggtgttcac
     1921 cctcgttcag ggcggggccg tctcgcgcct cggcgggttc ggacaacccg acgaagtcat
     1981 ccggctggcc acctccatga tcgacgtcgc cctagcggc
//
LOCUS       DAOOMA010000534         2018 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_70414_length_2018_cov_4.195619, whole genome shotgun sequence.
ACCESSION   DAOOMA010000534 DAOOMA010000000
VERSION     DAOOMA010000534.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 2018)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2018)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2018
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_70414_length_2018_cov_4.195619"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1263)
                     /locus_tag="PLP95_12170"
     CDS             complement(<1..1263)
                     /locus_tag="PLP95_12170"
                     /inference="COORDINATES: protein motif:HMM:TIGR02095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch synthase"
                     /protein_id="HPB46603.1"
                     /translation="MHVVFATAEIEPIVSTGGLGAASFGLVNALRRTGVEVTIVVPAY
                     APWDLPEERIVPLAVPAWVGQARARIGVHPAVGRIAMIEAPTIERPHPYVDPATGQGW
                     GDNDHRFFAWSAAVGALCHDLRSSGERVDVVHVNDWHAATTLAHLDESFRTVLSIHNL
                     AHQGWGGLGWMNALGHRGAAFVERGAVNALSGAVRLAERIVAVSPTYANEIRTFEGGM
                     GMDALLRDRGDAVVGILNGIDVDDWNPATDEALVSRFSVDDLESRSANHPALCAELGL
                     EVGPGPLVVSVSRFDGQKGIDMIPEVSHLLARIPTRMAFLGSGDKATEAAMSGVAAWN
                     PGRIAFRQGFDAALAHRMFAAADFAIVPSRFEPCGLTQMQAMRYGALPIVSDVGGLHD
                     TVVDVDDQPRRGVGVKLHAVSGAGIVDGV"
     gene            1310..>2018
                     /locus_tag="PLP95_12175"
     CDS             1310..>2018
                     /locus_tag="PLP95_12175"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012223100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'(2'),5'-bisphosphate nucleotidase CysQ"
                     /protein_id="HPB46604.1"
                     /translation="MIRHDTPPPEVDRADHQLASDLAEGAAELLVSLRDKLESDGEPS
                     YRIKALGDAVANRWILDRLAEVRPDDPVLSEEQYEHPEGVRARLNAAKTWIIDPLDGT
                     REFSEIPRVDWAVHIAIAVDHVAAAGAVSLPALGETYTTLHPAVVPPHDGPPRLVVSR
                     TRPAYEAMAIREALDGVLVPMGSAGAKTMAILRGEADIYAHSGGQYEWDSCAPVAVAA
                     AAGLWVSRLDGSPLRYNQ"
BASE COUNT          345 a          744 c          637 g          292 t
ORIGIN      
        1 caccccgtcg acgatgccgg ctccactgac cgcgtgcagt ttcaccccga cccctcgccg
       61 cggctgatcg tcgacgtcga cgacggtgtc gtgcagaccg ccgacatcgg acacgatcgg
      121 cagtgccccg tagcgcatgg cctgcatctg ggtgagcccg cacggctcga atctgctcgg
      181 aacgatcgcg aagtcggcag ccgcgaacat ccgatgggcc aacgccgcgt cgaatccctg
      241 gcgaaacgcg atgcggcccg gattccacgc ggccaccccc gacattgcgg cctcggtcgc
      301 cttatccccc gaccccagaa acgccatccg cgtcggaatc cgggccagca ggtggctcac
      361 ctcgggaatc atgtcgatgc ccttttgtcc gtcgaatcga ctcaccgaca ccacgagtgg
      421 gcccgggcca acctcgagac cgagctcggc gcacaacgcc gggtggttcg cagaccggga
      481 ctcgagatcg tcgaccgaga acctcgacac caacgcctcg tcggtggccg gattccagtc
      541 gtcgacgtcg atgccgttga ggatgccgac gaccgcgtcg ccgcgatccc gcaacagtgc
      601 gtccatgccc atgccgccct cgaaggtgcg gatctcgttc gcataggtcg ggctgaccgc
      661 gacgatccgt tcggccaggc gtaccgcccc cgacaacgcg ttgaccgccc cgcgttcgac
      721 aaacgccgcg ccacggtgac cgagcgcgtt catccagccg agaccgcccc acccctgatg
      781 ggcgaggttg tggatcgaca gcaccgtgcg aaacgactcg tcgagatgag cgagcgtcgt
      841 ggccgcgtgc cagtcgttca cgtgcaccac gtcgacccgt tcgccggagg agcgaaggtc
      901 gtgacacagc gcgccgactg cggccgacca cgcaaagaac cggtggtcgt tgtcgcccca
      961 cccctgcccc gtggcgggat cgacataggg gtgcgggcgc tcgatcgtcg gcgcctcgat
     1021 catggcgatc ctcccgaccg ccggatggac gccgatccgg gcccgagcct gcccgaccca
     1081 ggcaggaacc gcgagcggaa cgatccgttc ctcgggcagg tcccatggcg cataggcggg
     1141 tacgacgatg gtgacctcca cccccgtgcg gcgaagggca ttgacgagcc cgaatgacgc
     1201 cgcaccgagt ccgccggtgg acacgatcgg ctcgatctcc gcggtcgcaa acacaacgtg
     1261 caccgcacga ccgtattcgc ctcgccggtt tttggcagaa tccgaggcaa tgattcgcca
     1321 cgacacccca cctcccgagg tcgaccgggc cgaccatcaa ctcgcgtccg acctcgccga
     1381 gggggccgcc gagttgctcg tcagccttcg agacaagctc gagtccgacg gcgagccttc
     1441 gtatcgcatc aaggcgctcg gcgatgccgt ggcgaatcgc tggatcctcg accgactcgc
     1501 cgaggtgcgc cccgacgacc cggtcctcag cgaggaacag tacgaacacc ccgagggagt
     1561 gcgcgcccgg ctgaacgccg cgaagacctg gatcatcgac ccgctcgacg gaacccggga
     1621 gttctccgag atccctcgcg tcgactgggc ggtgcacatc gccatcgccg tcgatcacgt
     1681 cgccgccgcc ggagcggtct cgctgcccgc gctcggcgag acttacacca ccctgcatcc
     1741 ggcggtagtt cctccacacg acggtccccc gcgcctggtg gtcagccgga cccgtccggc
     1801 ctacgaagcg atggcgatac gcgaggcact cgacggagtg ctggtgccca tgggctcggc
     1861 cggagccaag acgatggcga ttcttcgcgg cgaggccgac atctacgccc actccggcgg
     1921 acagtacgag tgggactcgt gcgcgccggt ggccgtcgct gccgccgcag ggctgtgggt
     1981 ctcgcgcctc gacggcagcc cccttcgata caaccagc
//
LOCUS       DAOOMA010000535         2015 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_70580_length_2015_cov_4.246939, whole genome shotgun sequence.
ACCESSION   DAOOMA010000535 DAOOMA010000000
VERSION     DAOOMA010000535.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 2015)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2015)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2015
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_70580_length_2015_cov_4.246939"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..982
                     /locus_tag="PLP95_12180"
     CDS             <1..982
                     /locus_tag="PLP95_12180"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071111 - cyclic-guanylate-specific
                     phosphodiesterase activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GGDEF domain-containing phosphodiesterase"
                     /protein_id="HPB46605.1"
                     /translation="EPYEIDGQQLRLTVSAGIGVGVDGDEGAAEGLIRRSTAAMNRSR
                     GQGPGGVSTYDPSVEEQTAQRYALEQRLRGALERGEFQVHYQPEVDMVSGRVIGAEAL
                     LRWHCDGELQDAFRFVPVIEETGLIVPVGRWVLNEAVRQARVWRESIGDRAFVMRVNL
                     SARQLDDDNLVDQVAEALEREGFPAGCLCLEITESSLMSDVERSAELLGRLRALGVGL
                     AIDDFGTGYSSLSYLKRFPVDVLKVDRSFVTGVDHDAQDQAIVRSVVTLAEALKMSVT
                     AEGIETEAHARTLIELGCRRGQGWWYARAVDPATFESMLDAELSPVAFAT"
     gene            1066..1893
                     /locus_tag="PLP95_12185"
     CDS             1066..1893
                     /locus_tag="PLP95_12185"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipase"
                     /protein_id="HPB46606.1"
                     /translation="MRSTESSFVGAGGVRVYTVEWLPEGEPVADIVLIHGYGEHSGRY
                     LPWVERFVAAGYRVASLDHRGHGKTTGVKRGEVDSFDSLVDDLSAYVDAVRCERPLFL
                     YGHSMGGLAATRLAERGDERFAGIVLTGPALAVADAIPSVLVKVANVVGKLAPKLPTI
                     QLDGNAISRVQEVRDAYDADPLNFRGKMTAGTGRELNVNFAAAHAQAARISVPLLVLH
                     GEADALAAVEGSRNLVTEVSSTDVTLRTWPGAFHELHHEPERDEVFEVVSDWLAAHR"
BASE COUNT          320 a          609 c          720 g          366 t
ORIGIN      
        1 cgagccatac gagatcgacg gccaacagct ccgtttgacg gtgagcgctg ggatcggggt
       61 cggcgtcgat ggagacgaag gcgcggcgga gggcctgata cgacgcagca ccgccgccat
      121 gaaccgatcc cgcgggcagg gcccaggggg tgtgtcgacg tatgacccga gcgtcgagga
      181 acaaaccgcg cagcgctatg ccctcgagca gcggctgcgt ggtgcactcg aacgcggaga
      241 atttcaggtg cattaccaac ccgaggtcga catggtgtcg ggcagggtca tcggcgccga
      301 ggcactgttg cggtggcact gcgacggcga actgcaagac gcgtttcggt tcgtgccggt
      361 catcgaggag accgggctca tcgtgccggt cggccgttgg gtgctgaacg aagcggtccg
      421 gcaggctcga gtttggcgtg agtcgatcgg agatcgggcc tttgtcatgc gcgtcaacct
      481 gtcggcgcgc cagcttgatg acgacaacct cgtcgatcag gtggcggagg cgctcgagcg
      541 cgagggtttc ccggcagggt gcctgtgtct ggagatcacc gagtcgtcgc tcatgagcga
      601 tgtggagcgg tcggccgaat tgttgggccg gcttcgtgca ctcggcgtcg ggctggcgat
      661 cgacgacttc ggcaccgggt attcgtcgct gtcctatctc aaacggttcc ctgtcgatgt
      721 gttgaaggtc gaccggtcat tcgtcaccgg cgtcgatcac gatgcccagg atcaggcgat
      781 cgtgcggtcg gtggtcaccc tcgccgaggc gctcaaaatg tcggtgaccg ctgagggaat
      841 agagaccgag gcccacgctc ggaccctgat cgagctgggg tgccgccgtg gtcagggctg
      901 gtggtacgca cgagcggtgg atccggccac gttcgaatcg atgcttgacg cggaactgtc
      961 gccggtcgcc ttcgccacgt gatcgcccgg atcggtcgcg gcgacgtttc gcattgttga
     1021 ttcccgcccc gccgtgggtg gttgaaggcg cgtaacgtga gccgtatgcg cagcaccgaa
     1081 tcgtcctttg tcggagccgg gggtgtccgg gtttataccg ttgagtggtt gcccgagggg
     1141 gagccggtgg ccgacatcgt gctcatccac gggtacggcg aacactcggg tcggtacctg
     1201 ccctgggtgg aacgcttcgt ggcggcgggc tatcgggtcg catcgctcga ccatcggggc
     1261 catgggaaga caaccggcgt gaaacggggc gaggtcgact ccttcgactc gctggtcgac
     1321 gatctgtcgg cgtatgtcga cgcggttcgt tgcgaacgtc cgttgtttct ctacgggcac
     1381 tcgatggggg gcctcgccgc gacccgactt gccgagcgcg gcgacgagcg cttcgctggc
     1441 atcgtcttga cggggccggc ccttgccgtg gccgacgcga tcccgtccgt gctggtgaag
     1501 gtcgcgaacg tcgtgggcaa gctggcgccg aaactgccga cgatccagct cgacggcaat
     1561 gccatctcgc gggttcagga ggtgcgcgac gcctacgacg ccgacccgct caacttccgc
     1621 ggcaagatga ccgccggcac cggtcgggaa ctcaacgtga acttcgccgc cgcacacgca
     1681 caggccgcgc gaatctcggt gccgctgctg gtactgcacg gcgaggccga tgcgctcgcc
     1741 gccgtggaag ggtctcggaa cctcgtcacc gaggtctcct ccaccgacgt gacgttgcgg
     1801 acctggccgg gggcctttca cgaactgcac cacgaacccg aacgcgacga ggtgttcgaa
     1861 gtggtgagcg actggctcgc cgctcatcgt tagcgtcgct ccatccaacg cctgcggaag
     1921 cgttggggcc ggggcagtgg actcagttcg tgcctcagat ggtgtctcag tgggggtcga
     1981 ggacgacgac cgggatcaca cgacctgtcg atgct
//
LOCUS       DAOOMA010000536         2013 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_70671_length_2013_cov_3.972932, whole genome shotgun sequence.
ACCESSION   DAOOMA010000536 DAOOMA010000000
VERSION     DAOOMA010000536.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 2013)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2013)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2013
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_70671_length_2013_cov_3.972932"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..691)
                     /locus_tag="PLP95_12190"
     CDS             complement(<1..691)
                     /locus_tag="PLP95_12190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012231034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HPB46607.1"
                     /translation="MSDTTTELTSDDPTGEEFAYRQILDYDVSDADLEALYSSSMKGV
                     EDGQLVTGTVVKVDHDEVLLDIGYKSEGVIPARELSIRNDVDPHELVSIGDEVEALVV
                     TKEDKEGRLILSKKRAQYERAWGQIEQIKEADGVVSGPVIEVVKGGLIVDIGLRGFLP
                     ASLVELRRVRDLQPYVGQTLEAKIIELDKNRNNVVLSRRAWLEETQKEQRDEFLTNLK
                     PGERRRGVVSSV"
     gene            complement(769..1524)
                     /locus_tag="PLP95_12195"
     CDS             complement(769..1524)
                     /locus_tag="PLP95_12195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HPB46608.1"
                     /translation="MADRQRHWFEALADHMGPAYLRYSFAMGTAQEVDFIVEVLGLEA
                     GMRVLDVGCGPGRHSLELARRGFEVVGLDISATFIDLANADAAATTATFVCGDARNMS
                     FQAEFDAAISLCQGAFGLGGPPVSLDPSNLDNDLAVLRGIEAALRPGGVVGLSAFSSY
                     FQVRWLEDHDTFDAATAVNHETTEVRDPSGTAIPADLWTTCYTPRELRMIVERAGLEV
                     NDIFSVTPGDYVATAPSVHTPEFLVVAQRRRSA"
     gene            complement(1527..>2013)
                     /locus_tag="PLP95_12200"
     CDS             complement(1527..>2013)
                     /locus_tag="PLP95_12200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA polymerase"
                     /protein_id="HPB46609.1"
                     /translation="EAYGLAQRLNIATKEAQGILDAYFLAFPAVKDYMDRTVEEARER
                     GYTETLFGRRRRIPELSSGVRSVRMAGERQAMNAGIQGLAADIFKVALVRIDDAFEQG
                     DTGARLILQVHDEVICEVAPERLDEVQAQVIDIMRGAFAMAVPLEVNVASGDSWAAAK
                     G"
BASE COUNT          350 a          651 c          653 g          359 t
ORIGIN      
        1 cgacgctgga gaccacaccg cggcggcgct cgcccggctt gagattcgtg aggaactcgt
       61 cgcgctgttc cttctgggtc tcttcgagcc atgcacgacg gctgagcacc acgttgttgc
      121 ggttcttgtc gagctcgatg atcttcgcct cgagcgtctg gcccacatag ggctgaaggt
      181 cgcgcacccg gcgcaactcc accagcgaag ccggcaggaa gccacgaagg ccgatgtcga
      241 cgatgagacc gcccttgacg acctcgatga ccgggccgga cacgacgccg tcggcttcct
      301 tgatctgctc gatctgaccc cacgcacgct cgtattgcgc acgcttcttc gacaggatta
      361 ggcgaccttc cttgtcttcc ttggtgacga ccagcgcctc gacctcatcg ccgatcgaga
      421 cgagttcgtg cgggtcgacg tcgttgcgga tcgacagctc acgtgccgga atcacgcctt
      481 ctgacttgta cccgatatcg agcaggacct cgtcgtggtc gaccttgacg accgtaccgg
      541 tgacgagctg tccgtcttcg acacccttca tcgacgagga gtagagggct tcgaggtcgg
      601 cgtcggagac gtcgtagtcg aggatctggc ggtaggcgaa ctcctcgccg gtgggatcgt
      661 cggaggtgag ttctgtggtg gtatctgaca cgaaagagcc tgtagggatt gatggatagt
      721 ggatagagat gcgctgcgat acaacggtcg cagcaaagca cgaacaggct aggcgctgcg
      781 gcggcgttgc gccactacga gaaactcggg cgtgtgcacc gagggcgccg tggccacgta
      841 gtcgccgggg gtgaccgaga agatgtcgtt cacctccagg ccggcgcgtt cgacgatcat
      901 gcgaagttcc cggggcgtgt agcacgtggt ccacagatcc gcgggaatcg ccgtcccact
      961 ggggtcgcgc acctcggtgg tctcgtggtt caccgcggtc gcagcgtcga acgtgtcgtg
     1021 gtcctcaagc cagcgcacct ggaaatagga actgaacgca ctcagcccga cgacgcctcc
     1081 cggccgaagg gcggcctcga tgccgcgcag cacggccaga tcgttgtcga ggttggacgg
     1141 atcgaggctc accggtgggc cgccgagacc gaacgctccc tgacacagcg agatcgcggc
     1201 gtcgaattcc gcctgaaagc tcatgttgcg ggcgtcgccg cacacgaatg tcgcggtggt
     1261 cgccgcagcg tctgcgttcg cgaggtcgat gaacgtggcg ctgatgtcga gcccgaccac
     1321 ctcgaatccg cgacgagcga gttcgaggct gtgacggccc ggaccgcagc cgacgtcgag
     1381 cacgcgcatc cctgcctcaa gcccgagcac ctcgacgatg aagtcgacct cctgggcggt
     1441 acccatcgcg aaggagtacc gcagatatgc gggtcccatg tggtctgcca gggcctcgaa
     1501 ccagtgtcgt tggcgatcgg ccacggtcac cccttcgccg cggcccacga gtcgcccgac
     1561 gcgacgttga cctcgagcgg gacggccatg gcaaacgctc cgcgcatgat gtcgatgacc
     1621 tgcgcctgca cctcatcgag gcgttcgggg gcgacctcgc agatcacctc gtcgtgcacc
     1681 tgcaggatca ggcgcgcccc ggtgtcgccc tgttcgaacg cgtcgtcgat ccgcacgagc
     1741 gccaccttga agatgtctgc cgccagccct tgaatgccgg cgttcatggc ctgacgctcc
     1801 ccggccattc gtaccgaacg cacgccggaa ctgagctcgg gaatgcggcg ccggcgcccg
     1861 aaaagggtct cggtgtagcc gcgttcgcgg gcctcctcga cggtgcgatc catgtagtcc
     1921 ttcaccgcag gaaatgccaa gaagtacgca tcgagaatgc cctgggcttc cttggtcgct
     1981 atgttgaggc gctgggcgag cccgtaagcc tcc
//
LOCUS       DAOOMA010000537         2010 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_70833_length_2010_cov_3.353964, whole genome shotgun sequence.
ACCESSION   DAOOMA010000537 DAOOMA010000000
VERSION     DAOOMA010000537.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 2010)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2010)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2010
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_70833_length_2010_cov_3.353964"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            598..>2010
                     /locus_tag="PLP95_12205"
     CDS             598..>2010
                     /locus_tag="PLP95_12205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB46610.1"
                     /translation="MDPEPMTAVDDEVHEVGDPSEFEPVDEAASETVGVKSPSWTWWT
                     RERLFSLIVVAACCIYMVWAVHPELVLTDTTATGGDMGAHVWGPAFMRDFLLPQFAVT
                     GWSADWYAGFPAYLFYMVIPSLIIVILDVGIFDVTSIFGALASVVVVAGAIAAMWRIR
                     HHESRLVRFVVWVGAGLGALLLIDLPYNIAFKLVVVSGLIAFPAAVWYLMNALSLRRP
                     GPELGAIASLLFLMDRYLFNIYGGNMASTMAGEFAFSISITLALFGLGLVANGVRTGR
                     YRARAAMVIALSLMCHVIPGFFFLVTAAVIIVVLRPQVSSWKWALPTGLSAGLMGLWW
                     YLPFYGHSHFLNDMGWEKLGVQLRGESWARTQIGGALRTAGVEVASSNPVDAIRFDMN
                     WVQIRRYLLPFAPFEINGQTINDPNMHHGKIIFALAVVGAVMSLIMVVRAGIFFTLVT
                     AITAIAFIVMPQHRFWNARVL"
BASE COUNT          309 a          628 c          655 g          418 t
ORIGIN      
        1 cgcgatcaca acgaaactca ccggccggct cagcacggcg atcctggtgc acgccacgtt
       61 caacgcgatc accgcaattc cgctggtctt cggctgaggt gagccgtcgg acccggttga
      121 gggtcgggcg cactaaccgg ggcgggactg gcgtccgaca ccggacccgt cggggttgat
      181 ccgatcggat caaagcgccg caaggtgctt gaacccggcg cggtctcgac gatggagagg
      241 ttccactttc cactgagggg aacctgttca tgagtactgc acggaagcgg tcgacggtcg
      301 cgcaggatgc gcaaagcgcc gttcattcga aatcgccgga gcaccgctgt ggcggctgca
      361 atcaaatcgt ggtttcgatc aatctgctga tcgacgggga ggagttgacg atgcgttcct
      421 gttcgatctg tgatcggcgc agctggcacc gcggcggtga accggtgaat ctcagtggta
      481 tcctcgccga cttgagttcc tccccgacgc gttaccggcg ggatctcgcg agccgctgaa
      541 cgctccggtg tggcggcaaa acctcgaccc gtcgaccaca caggtaagaa tcgaccgatg
      601 gacccagagc cgatgaccgc cgtggacgac gaggtgcacg aggtcggaga cccttcggaa
      661 ttcgaaccgg tcgacgaggc cgcctccgaa acggtggggg tgaagagccc ctcctggaca
      721 tggtggacga gggagcggtt gttcagcctc atcgtcgtcg ctgcctgttg catctacatg
      781 gtgtgggcgg ttcacccaga gctcgtcctc accgacacca ccgccaccgg cggtgatatg
      841 ggtgcgcacg tgtggggccc ggcgttcatg cgcgacttcc tgttgccgca gttcgcggtg
      901 accggctgga gcgccgactg gtatgccggg ttccccgcct acctcttcta catggtcatc
      961 ccgtcgctga tcatcgtgat cctcgacgtc ggcatcttcg acgtcacttc cattttcggg
     1021 gcgctggcat cggtcgtcgt ggtggccggg gccatcgccg cgatgtggcg gatccgccat
     1081 cacgagtcgc ggctcgttcg cttcgtggtg tgggtcggtg cgggcctcgg cgcactgttg
     1141 ttgatcgatc tgccctacaa catcgccttc aagttggtgg tcgtgtcggg gctcatcgcg
     1201 ttccccgccg cggtgtggta cctgatgaac gctctgtcgc tgcgtcgccc gggccccgaa
     1261 ctcggggcga tcgcttcgct gctgttcttg atggaccgct acctgttcaa catctacggc
     1321 ggcaacatgg cctcaacgat ggccggggag ttcgcgttct cgatctcgat caccctcgca
     1381 ctgttcggct tgggcctcgt tgcgaacggt gttcgaacgg gccggtatcg ggcccgtgcc
     1441 gcgatggtca tcgcgttgtc gttgatgtgc cacgtcattc ccgggttctt cttcctcgtc
     1501 accgcggcgg tcatcatcgt ggtgttgcgc ccgcaggtgt cgtcgtggaa gtgggcgctg
     1561 ccgacggggt tgagcgccgg gttgatgggc ctgtggtggt acctgccgtt ctacggtcac
     1621 agccacttcc tcaacgacat gggctgggag aagctcggtg tccagttgcg cggggagagt
     1681 tgggcccgca cccagatcgg tggcgcactg cgaactgcgg gggtcgaggt ggccagttcg
     1741 aatcccgtcg acgccattcg cttcgacatg aactgggtgc agatccgtcg ctacctgttg
     1801 cccttcgccc cgttcgagat caacggccaa acgatcaacg accccaacat gcaccacggc
     1861 aagatcatct tcgccctggc ggtggtcggc gcggtgatgt cgctgatcat ggtggtgcgc
     1921 gccggcatct tcttcaccct cgtcaccgcc atcaccgcga tcgcgttcat cgtgatgcca
     1981 cagcaccgct tctggaacgc ccgggtgctg
//
LOCUS       DAOOMA010000538         1998 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_71416_length_1998_cov_3.522903, whole genome shotgun sequence.
ACCESSION   DAOOMA010000538 DAOOMA010000000
VERSION     DAOOMA010000538.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 1998)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1998)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1998
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_71416_length_1998_cov_3.522903"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            90..650
                     /locus_tag="PLP95_12210"
     CDS             90..650
                     /locus_tag="PLP95_12210"
                     /inference="COORDINATES: protein motif:HMM:NF013077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase"
                     /protein_id="HPB46611.1"
                     /translation="MSVEFTDLQALVRTRRTTKQTDSDREVPRTVVEDLCELATWAPN
                     HRRTNPWRFAVFTGAARQRLGDTIGDVLVKLEAHEAAIKKTRTKYFRASTIIVVGSIE
                     GPDEVTTGENRDSVSAGINNMLLGAHALGLATLWSTIATPTAPELLALCGFPPGTFCA
                     GAIYLGYPTGSEGAGVREAPTITWHE"
     gene            complement(663..1307)
                     /locus_tag="PLP95_12215"
     CDS             complement(663..1307)
                     /locus_tag="PLP95_12215"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HPB46612.1"
                     /translation="MIVSSDIAESVVAHSTARDEVQRELAQVTAERTGRAAGMQIGHD
                     QALFMEMLTRAIGARRAIEIGTFTGYSSLAIARGLGPEGHLTCCDVSEEWTSTAREFW
                     RRAGVEDRIDLRIAPALETIASFGAEVRFDLAFIDADKENYAAYLDALDPHLPAGAIV
                     LIDNTLWSGAVLDPPSDDASAQALAALNDALVDGERFTVAMLTIGDGVTMLQKR"
     gene            1488..>1998
                     /locus_tag="PLP95_12220"
     CDS             1488..>1998
                     /locus_tag="PLP95_12220"
                     /inference="COORDINATES: protein motif:HMM:NF012336.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HPB46613.1"
                     /translation="MASEVYIVSGVRTPIATAYKGSLGGVDAFELAQTVIAAAVERSG
                     VDASQFEDMGFGESFQGGGNIGRNAAIRAGLTNIPGVATQRWCASGMAGVQWVAANIT
                     AGMIDAGVAGGVESMTTAPGTSRGGEFWLSPANEPTELAPPFNTALGVGDNAARIAGV
                     TREQADEWAF"
BASE COUNT          343 a          687 c          645 g          323 t
ORIGIN      
        1 ctgtcgaagc cctcgaacat gcgtttgata ctaaacgcga cccctgacaa cgaagttggt
       61 gactccccgg acgcgccaaa ctggtggtca tgtccgtcga gttcaccgat ctccaagcgc
      121 tggtgcgcac ccgtcgcacg accaaacaga ccgattccga tcgcgaggtg ccccgtacgg
      181 tcgtcgagga cctgtgcgaa ctggcgacct gggcccccaa tcaccggcga accaacccgt
      241 ggcgctttgc cgtgttcacc ggagctgcac gccaacgtct cggcgacacg attggcgacg
      301 tgttggtcaa actcgaggcg cacgaggccg ccatcaagaa gacccgcacc aagtacttcc
      361 gggcttcaac catcatcgtc gtcggttcga tcgaaggacc cgacgaggtc accaccggcg
      421 aaaaccgcga ctcggtatcg gccgggatca acaacatgct gctcggagcg catgcgctcg
      481 ggctcgcgac gctgtggtcg acgatcgcca ccccgaccgc tcccgaactg ctcgcgcttt
      541 gcgggttccc gcccggcacg ttctgcgccg gggcgatcta cctgggttac ccgaccggtt
      601 ccgagggcgc cggcgtgcgc gaggccccga ccatcacctg gcacgaatga cccaccgagc
      661 gctcagcgct tctggagcat cgtcaccccg tcgccgatgg tcaacatcgc gaccgtgaac
      721 cgctcaccgt cgaccaacgc atcgttgagc gcagcgagcg cttgggcgga cgcatcgtcg
      781 ctgggtgggt cgagcaccgc ccccgaccac aaggtgttgt cgatgagcac aatcgccccg
      841 gcgggcagat ggggatcgag cgcgtcgagg tacgcggcgt agttctcctt gtcggcgtcg
      901 atgaacgcca ggtcgaagcg cacctccgca ccgaacgacg cgatcgtctc gagcgcgggc
      961 gcgatgcgta gatcgatccg gtcctccacc cccgctcgcc gccagaattc gcgggccgtc
     1021 gaggtccact cctcgcttac gtcacaacag gtcaggtgac cctccggacc gagaccgcgg
     1081 gcgatggcga gggacgagta cccagtgaag gtgccgatct cgatcgcccg acgggctccg
     1141 atggcgcggg tgagcatctc catgaacagg gcctggtcgt ggccgatctg catgccggca
     1201 gcacggccgg tgcgttcggc ggtgacctgt gcgagctcgc gctgcacctc gtcgcgagcg
     1261 gtgctgtgcg cgacgacgga ctcggcgatg tcggatgaga caatcaccct tcgagtgctg
     1321 gccactggtc ctccccggcc gccccggtac ggcattgcgc aggaacgagc gaggcgatcc
     1381 taggccgagc ccagcttcac gtcgttgcgt ggcgcatgac acaatcgaac gcactgctga
     1441 tctgacgaag cgtcaggtca cccgtccact cgtaggagtt tcccgtcatg gcatccgagg
     1501 tctatatcgt cagcggcgtt cgcacgccga tcgccaccgc ctacaagggc tcgctcggcg
     1561 gggtcgatgc gttcgaactc gcccaaactg tgattgccgc agcggtcgag cgttccggtg
     1621 tggacgcctc ccagttcgaa gacatgggct tcggtgagtc gttccagggt gggggcaata
     1681 tcggccgcaa cgccgcgatc cgtgccggtc tcacgaacat cccgggcgtc gccacccaac
     1741 gctggtgtgc ttcgggaatg gccggcgtgc agtgggtcgc cgccaacatc accgcgggca
     1801 tgatcgacgc cggtgtcgct ggcggtgtcg agtcgatgac gaccgctccg ggcaccagcc
     1861 gcgggggcga gttctggctg tccccggcca acgagccgac cgagctcgca ccgccgttca
     1921 acacggcgct cggggtcggc gacaacgccg cccgcatcgc cggggtgacc cgcgagcagg
     1981 ccgacgaatg ggcgttca
//
LOCUS       DAOOMA010000539         1996 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_71501_length_1996_cov_3.872746, whole genome shotgun sequence.
ACCESSION   DAOOMA010000539 DAOOMA010000000
VERSION     DAOOMA010000539.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 1996)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1996)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1996
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_71501_length_1996_cov_3.872746"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..874
                     /locus_tag="PLP95_12225"
     CDS             <1..874
                     /locus_tag="PLP95_12225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB46614.1"
                     /translation="PRTQYYEMIGSRALYHDGWKAVTYHPPQIANYEGISAATFDDDR
                     WELYHVAEDFSECHDLAESEPDKLAELQELWWREAKRFNVLPLNNEPGVHGDGRYKRM
                     AYRYHGRVGPLSEAIAPNLKNREWTIEADLRLNRRDPSSPVAGVIAAHGSHAGGWVLF
                     VHDDRLMFTYNYLDTERSTATAEVALPATDVTVRANFVRDPFGAGGTVELFYGDVPVG
                     SALVAATTPLTYGTAGFTIGFLPSSPIDHRLSARAELDPEVLGEVRFELRGVDPIRDS
                     MAPPIDNRVDLATQ"
     gene            complement(936..1868)
                     /locus_tag="PLP95_12230"
     CDS             complement(936..1868)
                     /locus_tag="PLP95_12230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB46615.1"
                     /translation="MTEQQPSEPTEQRPTDSGTDSGTDSGTAGRAGISGRRAVGILGI
                     LVVVLVGVLAWMTLRDNARPVDIEDAKNRISTTVDLEAKGEQGEGEQGPTPSTERVRP
                     APGIYRFTGEGTESLSTPPLSQSQGPEMPATVEHDGDDCWTFRIDYSTNHWQQWRYCW
                     SGDDLVETGGTTWQRWVIGPTSIANTGVFECTDETVVIPAERGDGASWTGTCVGTNDA
                     AEGEMTSTGPYRFAGETTLAVGDTNLDVLHFVRERTMSGAQTGTERTDVWFDAETGLP
                     VRNERNLEAETDTVIGASTYTEQGWFQLAELGTL"
BASE COUNT          328 a          693 c          624 g          351 t
ORIGIN      
        1 gcctcggacg cagtactacg agatgatcgg atcacgagcg ctgtatcacg acggctggaa
       61 ggcggtgacc tatcacccgc cacagatcgc caactatgag ggcatcagcg ccgccacctt
      121 cgacgacgac cgttgggagc tctatcacgt cgccgaggac ttctcggagt gtcacgatct
      181 ggccgagtcc gaacccgaca aactcgccga attgcaggag ctctggtggc gcgaggccaa
      241 gcgcttcaac gtgttgccgc tgaacaacga acccggggtg cacggcgacg ggcgatacaa
      301 gcgaatggcg taccgctacc acggacgcgt cgggccgctg tcggaggcga tcgctccgaa
      361 tctcaagaac cgcgagtgga ccatcgaggc cgacctgagg ttgaaccgac gcgatccgtc
      421 gtcgccggtt gccggggtga tcgcggcgca cggcagccac gccggcggct gggtgctgtt
      481 cgtgcacgac gatcggctga tgttcaccta caactatctc gacaccgaac gcagtacggc
      541 gacggcggag gttgcgcttc ctgccaccga tgtgacggtc cgcgccaatt tcgtgcgcga
      601 cccgttcggc gccggcggga cggtcgagct gttctacggc gacgtgccgg tcgggtcggc
      661 cttggtggcc gccaccactc ccctgaccta cggcacggcc ggattcacta tcgggttcct
      721 tccgtcgagc cctatcgatc accgcctcag cgcacgcgcc gaactcgacc ccgaggtgct
      781 cggtgaggtg cgattcgaac tgcgcggcgt cgaccccata cgcgactcga tggctccccc
      841 gatcgacaac cgggtcgatc tcgccaccca gtaggaccac tcagtaggac cactcatacc
      901 gtgcggatca ggccgtaccg atcagaccgt gccgatcaga gcgtgccgag ttcggcgagt
      961 tggaaccatc cctgctcggt ataggtcgag gccccgatga cggtgtcggt ttcggcctcg
     1021 aggttccgct cgttgcgcac gggcagaccc gtctccgcgt cgaaccacac gtcggttcgc
     1081 tcggttccgg tctgggctcc gctcatcgtg cgctcccgca cgaaatgcaa cacgtcgaga
     1141 tttgtgtcgc cgaccgccag cgtcgtctcg ccggcgaaac ggtaggggcc ggtcgaggtc
     1201 atctcaccct cggccgcatc gttggttccg acacaggttc cggtccacga ggccccatcg
     1261 cctcgttcgg ccggaatcac gaccgtctcg tcggtgcact cgaagacacc ggtgttcgcg
     1321 atcgatgtcg ggccgatcac ccaacgctgc cacgtcgttc cacccgtctc gacgagatcg
     1381 tccccgctcc agcagtagcg ccactgctgc cagtggttgg tgctgtagtc gatgcggaag
     1441 gtccagcagt cgtcgccgtc atgttcgacc gtcgcgggca tctccggacc ctgggattgc
     1501 gacagcggcg gtgtcgacag cgactcggtc ccctctccgg tgaaccgata gatgccgggt
     1561 gccggacgaa cgcgttccgt cgagggggtg gggccctgct cgccctcacc ctgctcgccc
     1621 ttggcttcga ggtcgacggt ggtggagatc cggttcttgg cgtcctcgat gtcgaccggc
     1681 cgggcgttgt cccgcaacgt catccatgcg agcaccccga cgagcaccac cacgaggatc
     1741 ccgaggattc ccacggccct gcgtcctgag attcccgccc tgcccgcggt gccagagtcg
     1801 gtgccagagt cggtgccgga gtcggtcggg cgctgttcgg tcggttcgct cggttgctgc
     1861 tcagtcacgg ggatgccacc aatcgtcttc gccggctgcc gggagcctag ccaccgacca
     1921 acacccgcac gccaacaccc gcaccccaaa ccccgccgcc aaacccgttc tccgataacc
     1981 agtggtcgtg atcacg
//
LOCUS       DAOOMA010000540         1992 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_71712_length_1992_cov_3.488900, whole genome shotgun sequence.
ACCESSION   DAOOMA010000540 DAOOMA010000000
VERSION     DAOOMA010000540.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 1992)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1992)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1992
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_71712_length_1992_cov_3.488900"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1067
                     /locus_tag="PLP95_12235"
     CDS             <1..1067
                     /locus_tag="PLP95_12235"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HPB46616.1"
                     /translation="GGGRLARSRRSAGTQHRAGTQHRAVISEVIPPALSGERIDRVVA
                     MLTGCTRAESAAAIAAQTVRIDDKVITKPSARVRTDQIVIVSEDPHIAEQPPVADASV
                     EVPVVYADESVIVIDKPAGMIVHPGAGHDELTMVNGLLARFAELEDVGERHRPGIVHR
                     LDKGTSGLMVVARTPQAYDDLVEQLATHAVEREYEALVWGLVEPATGTIDAPVGRSRR
                     NPLLMTVSADGRFARTHYEVIEQFNDPQPMTRLRLRLETGRTHQIRVHLRSIGHPVAG
                     DDVYGGRRPLLGITRPFLHARRLLFTHPSTGDDVEFHSELAEDLGALLGTLSWLTDAP
                     VEELPDPFEGYDADGQPLEG"
     gene            complement(1098..1544)
                     /locus_tag="PLP95_12240"
     CDS             complement(1098..1544)
                     /locus_tag="PLP95_12240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HPB46617.1"
                     /translation="MKVSTRGDYASRALLSLALHESDGPTSVRDIAERTGLPQPYLEQ
                     ILLALKGAGLVRSKRGVGGGYVLARPPEEITLAEVVSAVDGPIQAGDFGKPHTNGSCD
                     HEGQCVLLAIWGDVGDKMQQALTAYTLADISAISAGDRPWPESAGT"
     gene            1651..>1992
                     /locus_tag="PLP95_12245"
     CDS             1651..>1992
                     /locus_tag="PLP95_12245"
                     /inference="COORDINATES: protein motif:HMM:NF013799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HPB46618.1"
                     /translation="MTSDSIPQPVLPDYGGACNAGIIPAVLGDPEEAPSWMPRGAIDA
                     DTTIVLVLDGLGWEQMQQRLSLLPTLAQFEAMPITTVAPSTTATALTSITTGLTPGEH
                     GVIGYRIAIDRD"
BASE COUNT          339 a          681 c          668 g          304 t
ORIGIN      
        1 ctggcggtgg ccgtctggcg cgctcccgaa gaagcgccgg aacccagcac cgagccggaa
       61 cccagcaccg agccgtgatt tccgaagtca tcccgccagc gctctcgggc gagcgaatcg
      121 accgggtcgt cgcgatgctc accgggtgta cccgggcgga atcggccgca gcgatcgccg
      181 cccagaccgt gcgaatcgac gacaaggtca tcacgaaacc ctcggcgagg gtgcgcaccg
      241 atcagatcgt cattgtcagt gaggatcccc acatcgccga gcaaccgccg gtggccgacg
      301 cctcggtcga ggtcccggtc gtctacgcgg atgagtcggt catcgtcatc gacaagcccg
      361 cgggcatgat cgtgcatccc ggagcgggcc acgacgaact cacgatggtc aatggcctgc
      421 tggcccgctt cgctgaactt gaagacgtgg gggagcgcca ccggccggga atcgtgcacc
      481 gcctcgacaa gggcacgtcg gggctcatgg tcgtcgctcg gacaccgcag gcctacgacg
      541 acctcgtcga gcaactcgca acccacgcgg tcgaacgaga atatgaggcg ttggtgtggg
      601 ggctggtcga acccgccacc ggcacgatcg atgcccccgt cgggcgatcc cggcgcaatc
      661 cgttgctcat gaccgtcagc gccgatggcc gcttcgccag aacccactac gaggtcatcg
      721 agcagttcaa cgatccgcag ccgatgaccc gtttgcgcct ccggctcgaa accggtcgaa
      781 cccaccagat tcgggtccac ctgcgcagca tcggccatcc cgtcgccggc gacgacgtct
      841 acggcgggcg ccgcccactg ttgggcatca cccgaccgtt cctccacgct cgtcggctgc
      901 tgttcaccca cccgagcacc ggtgacgacg tcgagttcca cagcgaactc gccgaggacc
      961 tcggagcgct tctgggcacg ctgtcgtggc ttaccgacgc ccccgtcgag gaactccccg
     1021 atccgttcga gggatatgac gccgatgggc aaccgctcga ggggtgaggc cccggccgag
     1081 ccgacgcagc ggcggcctca ggtgccggcg gattccggcc aggggcgatc gcctgcgctg
     1141 atggccgaga tgtcggcgag ggtgtaggcg gtgagcgcct gttgcatctt gtcgccgacg
     1201 tctccccaga tcgccaacaa cacgcactgc ccttcgtggt cacatgagcc gttggtgtgg
     1261 ggtttgccga aatcgcccgc ctgaatcgga ccgtcgacgg cgctcacgac ctcggcgagg
     1321 gtgatctcct cgggcggacg ggcgagcaca tagccgccgc cgacgccacg cttggaacga
     1381 acgaggccgg cgcccttcag cgccaggagg atctgttcga gatacggctg cggcaggccg
     1441 gtgcgctcgg cgatatcgcg caccgaggtc ggaccgtcgg actcgtggag cgcgagcgac
     1501 agcagtgctc gtgaggcgta gtcgccgcgg gtggaaacct tcacggcgcc aatcgtaggc
     1561 cgaagcgtgg caagcgggtg tcggcgccga tgcggagagg gtgcgcagtc ggtgtccgtg
     1621 tcggcgccgg cggcactacc gtgggggtca gtgacgagcg actccattcc acaacccgtg
     1681 cttcccgact acggcggggc atgcaacgcc ggaatcatcc cggccgtcct gggcgatcca
     1741 gaggaggctc cctcctggat gcctcgcggc gcgatcgatg ccgacacgac gatcgtgctg
     1801 gtgctcgacg gactcggctg ggaacagatg caacagcgtc tgtcgttgtt gcccacgctg
     1861 gcgcagttcg aggcgatgcc gatcaccacc gttgccccgt cgaccaccgc gacggcgctg
     1921 acctcgatca cgacggggtt gacgcccggc gaacacggtg tgatcgggta ccgaatcgcc
     1981 atcgatcgcg ac
//
LOCUS       DAOOMA010000541         1992 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_71715_length_1992_cov_3.418172, whole genome shotgun sequence.
ACCESSION   DAOOMA010000541 DAOOMA010000000
VERSION     DAOOMA010000541.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 1992)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1992)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1992
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_71715_length_1992_cov_3.418172"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..403)
                     /locus_tag="PLP95_12250"
     CDS             complement(<1..403)
                     /locus_tag="PLP95_12250"
                     /inference="COORDINATES: protein motif:HMM:NF014626.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0018142 - protein-DNA covalent
                     cross-linking [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HPB46619.1"
                     /translation="MCGRFVAATPVEELAEYFGASAPAEPVPAQYNIAPSADVEVVVS
                     DAQGSRHIELYRWGLVPVWAKDLNVGNRMINARSETAAEKNSFKSSLSKKRCIVPADG
                     FYEWAAVAGRKAKQPYFIRRADGAPLAFAGLA"
     gene            473..1663
                     /locus_tag="PLP95_12255"
     CDS             473..1663
                     /locus_tag="PLP95_12255"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HPB46620.1"
                     /translation="MNDTAPDRRSDRPDRRRAPELSVITPTYNEADNIEVLLDALEVA
                     LVTVPHEVIVVDDDSPDRTWERAERYAESHRNVRVLRRFSDHGLSSAVMAGMEASNGE
                     VIAVIDADLQHDETALVAMVDMIRDGSADIVVGTRGAEGGSYGDWSVFRRFVSWVAAM
                     IAKVFLRVQTSDPMSGFFAISRDAYLRSAPEVNPRGFKILLEFIGRNRDLRVGEVGYE
                     FRNRVAGETKMSPSIIRSYLLAVFELRFGRQVKGQFFLYSLVGASGVVVNLAVFSLLE
                     VAGVGTIDLGFSRPVRWSLLGGIAASIVWNFLLNNYFTFWEQRFHRRNLLGGLALFSA
                     VSLLGVVIHVAVFQFLQNNGWGRSVLGPALTDIVQDAFGYVVALISNYFLNVNYIWRR
                     RPED"
     gene            complement(1698..>1992)
                     /locus_tag="PLP95_12260"
     CDS             complement(1698..>1992)
                     /locus_tag="PLP95_12260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443375.1"
                     /note="Presence of a B(12) (cobalamin)-binding domain
                     implies dependence on cobalamin itself, in one of its
                     several forms, or in some unusual lineages, dependence on
                     a cobalamin-like analog; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cobalamin-dependent protein"
                     /protein_id="HPB46621.1"
                     /translation="ALDSGQLSVREVVAAAIDEDVDVIGLSMLSGAHLALVPPVLEHL
                     TANGVDIPVVVGGIIPDRDLPTLLDAGVAAVLAPGASEEEIVATMQRVVAGRG"
BASE COUNT          308 a          677 c          656 g          351 t
ORIGIN      
        1 ccgccagccc ggcgaaggcc agcggggcac cgtcggcacg gcggatgaag tacggctgct
       61 ttgccttgcg accggcgacc gctgcccatt cgtagaagcc gtcggcgggc acgatgcagc
      121 gcttcttcga cagcgaggac ttgaagctgt tcttctcggc ggcagtttcg ctgcgggcgt
      181 tgatcatccg gttgccgacg ttgaggtcct tggcccacac cggtacgagg ccccagcgat
      241 acagctcgat gtgacgcgac ccctgagcgt cgctgaccac gacctcgacg tccgcgctcg
      301 gagcgatgtt gtactgggcc ggtaccggct cggcgggagc gctcgccccg aagtactcgg
      361 ccagttcctc gaccggggtc gccgcgacaa aacgtccaca catgtgcacg acgctagtgc
      421 cgtggccgcg cacccacgtc ggcgccggcg gccccgctag cgtctgaaca tcgtgaacga
      481 cactgccccc gatcggcgct ccgaccgccc cgaccgacgc cgcgcccccg agttgtcggt
      541 gatcaccccg acctacaacg aggccgacaa catcgaagtg ttgctcgatg cgctcgaggt
      601 ggcactcgtc accgtgcccc acgaggtcat cgtggtcgac gacgactccc ccgatcgcac
      661 ctgggagcgg gccgagcgct acgccgagtc gcatcgcaac gtccgggtgc ttcgccgctt
      721 cagcgatcac ggactgtcga gcgcggtcat ggccggcatg gaggccagca acggcgaggt
      781 gatcgcggtc atcgatgcgg atctccaaca cgacgagacc gcgctcgtgg cgatggtcga
      841 catgatccgc gacggttcgg ccgacatcgt cgtcggcacg cggggtgccg agggcggcag
      901 ctacggcgac tggtcggtgt ttcgccgatt cgtctcctgg gtcgccgcca tgatcgccaa
      961 ggtgttcctc cgggtgcaga catcggatcc catgtcgggg ttcttcgcca tctcccgcga
     1021 cgcctatctg cgcagtgcgc cggaggtgaa cccgcgcggg ttcaaaatcc tcctcgagtt
     1081 catcggccgg aaccgggacc tgcgcgtggg cgaggtgggc tacgaattcc gcaatcgggt
     1141 cgccggcgaa acgaagatgt cgccgtcgat catccgctcc tatctcctcg cggtgttcga
     1201 gctgcgtttc ggcaggcagg tgaaggggca gttcttcctg tacagcctgg tgggagcgtc
     1261 gggggtggtg gtgaacctcg cggtgttctc actgctcgag gtcgcgggcg tcggcacgat
     1321 cgatctcggc ttcagccgac ccgtgcgctg gtcgttgctc ggcggcatcg cggcgtcgat
     1381 cgtgtggaac ttcttgttga acaactactt cacgttctgg gagcaacggt ttcaccgccg
     1441 caacctgctc ggcggcctcg cgttgttcag cgcggtctcc ctgctcggcg tcgtcatcca
     1501 cgtggcggtg tttcagtttc tccagaacaa cggatggggc cgctcggtgt tggggcctgc
     1561 gctcaccgac atcgtccagg acgcgttcgg ctatgtcgtc gcgttgatca gcaattactt
     1621 cctcaacgtg aattacatct ggcggcgacg ccccgaggac tgagaagccc gacgcgttcg
     1681 gggctgcgtt cagcgactca accgcgtccg gcgaccacgc gctgcatcgt cgcgacgatc
     1741 tcctcctcgg aggcaccggg ggccaacacc gcggcaaccc cggcgtcgag cagcgtcggc
     1801 aggtcgcgat cggggatgat gccgccgacc accacgggga tgtcgacccc gttcgccgtc
     1861 aggtgctcga gcacgggcgg caccagcgcg agatgcgcgc ccgacagcat cgacagcccg
     1921 atcacatcga cgtcctcgtc gatcgccgcg gcgaccacct cccggaccga cagctggccc
     1981 gagtcgagcg cg
//
LOCUS       DAOOMA010000542         1979 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_72367_length_1979_cov_3.134096, whole genome shotgun sequence.
ACCESSION   DAOOMA010000542 DAOOMA010000000
VERSION     DAOOMA010000542.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 1979)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1979)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1979
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_72367_length_1979_cov_3.134096"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1284)
                     /locus_tag="PLP95_12265"
     CDS             complement(<1..1284)
                     /locus_tag="PLP95_12265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB46622.1"
                     /translation="MKRAISPRFSDVASATAVLSPSAVGPTAGQRDPSPSGTPADDAL
                     EHTALEHTALGHTALGHTTNTDAGAVPDGPFSAPPAPPGDPGPDVTATTSSHDRLSVF
                     NPMIAAVRLSTVAIGLLLAATTLIDGDLATVVGTVVVAGYSTYRAIRPVRDGSPSAID
                     RQLLLESLVLTSVVAATGFWTSPFVFSLLPIIVISGFAGGFGYALRAAAGLAIIVGVP
                     TILVEENHTEALTLSGTWTTLVLLIALVAGFGRKLSGQADRERDLAFDRLSRLADANT
                     LLYSLHKVTQTLPSSLDMHDVVVSTVTQLRELFEFDSVAIVLFDETAGDWNVIRQEGC
                     HLPDRLGSDELPPGLRQAITTAGVVRLARLGAIDGDGLAERSASGLYSALVARGTTIG
                     LIAVEHRRPDAFDLRCANVLSGVVPALAMALDNAQW"
     gene            1358..>1979
                     /gene="coaE"
                     /locus_tag="PLP95_12270"
     CDS             1358..>1979
                     /gene="coaE"
                     /locus_tag="PLP95_12270"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012228820.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HPB46623.1"
                     /translation="MILVGLTGGIGSGKTTVSAGLAARGAVVIDADRIVHELQSPGQQ
                     VLAEMVERFGEEILHPDGTLNRQAVADRVFGDEEALADLGKIVHPRVSEEIFRRVAEQ
                     DHTDNIVVLDIPLLTESGWEGMVGTIVVDLDTELAVQRLMAHRSFPEADARARIARQA
                     TREERRAGAWIVIDNSGDFDDLERLIDDAWEQILAKRDEVLAAGFHG"
BASE COUNT          336 a          605 c          726 g          312 t
ORIGIN      
        1 ccactgggcg ttgtcgaggg ccatcgccaa cgccggcacc acgccggaca agacgttggc
       61 gcagcgcagg tcgaacgcgt cgggccgacg gtgttcgacg gcgatcagtc cgatggtcgt
      121 gccgcgggcg acgagcgccg agtagaggcc cgatgcggac cgttcggcca gcccgtctcc
      181 gtcgatcgct ccgagacggg cgaggcgcac gacgccggcg gtggtgatcg cctgtcgaag
      241 cccggggggc aactcgtcgg aaccgagccg atcggggagg tggcatccct cctggcggat
      301 gacgttccag tcgccggcgg tctcgtcgaa caagacgatg gccaccgagt cgaactcgaa
      361 caactcgcgc aactgcgtca cggtggacac cacgacgtcg tgcatgtcga gcgatgacgg
      421 aagcgtctgg gtgaccttgt ggagcgagta gagcagcgtg ttcgcatcgg ccaggcgact
      481 gaggcggtcg aaggccaggt cgcgttcgcg gtccgcctgt ccggagagtt ttcgtccgaa
      541 gcccgcgacg agggcgatca ggagcaccag ggtggtccac gtgccggaca gcgtgagcgc
      601 ttcggtgtgg ttttcctcga ccaggatcgt ggggactccg acgatgatgg ccagtccggc
      661 cgcggcgcga agcgcgtagc cgaatccgcc cgcgaagcca ctgatcacga tgatcggcag
      721 gaggctgaac acgaacggac tcgtccagaa tccggtcgcc gccacgacgc tggtcaacac
      781 cagggactcg aggagcagtt ggcgatcgat cgccgacggg gaaccatctc gaacgggacg
      841 gatcgcccgg taggtcgaat agccagcgac gaccacggtg ccgacgacgg tggccaggtc
      901 gccgtcgatg agcgtcgtgg cggccaacag cagcccgatt gcgacggtcg agaggcgtac
      961 cgcggcgatc atcgggttga agacgctcaa ccggtcatgg ctgctggtgg tggcggtgac
     1021 gtcggggccg gggtctccgg gtggcgccgg cggggcggag aacggaccgt caggaaccgc
     1081 tccggcgtcg gtgttcgtgg tgtgtccgag tgccgtgtgt ccgagtgccg tgtgttcgag
     1141 cgccgtgtgt tcgagtgcgt catcggccgg agtccccgag ggcgacggat cacgctgccc
     1201 cgccgtgggc ccgacggcgc tcgggctcag cacggccgtc gccgatgcga cgtcggagaa
     1261 cctgggggaa attgcccgct tcatcgctgg ttctatcggc agcttcgggc agaacatgag
     1321 tcgtgtgact caccgggggg ttcgataatc tccccgcatg attctcgtgg ggctcaccgg
     1381 aggcatcgga tcgggaaaga cgacggtgtc cgcggggctc gccgcacggg gtgccgtggt
     1441 catcgacgcc gaccggatcg ttcatgaact gcaatcgccg ggccaacagg tgctcgcgga
     1501 gatggtcgag cgcttcggcg aagaaatcct gcatcccgac ggcacgttga atcgccaggc
     1561 ggtggccgac cgcgtgttcg gcgacgagga ggcgttggcc gacctcggca agatcgtgca
     1621 tccgagggtg tcggaggaga tctttcgccg cgtcgccgaa caggaccaca ccgacaacat
     1681 cgtcgtgctc gacatcccgt tactcaccga atccggctgg gagggcatgg tgggcacgat
     1741 cgtcgtcgat ctcgacaccg agttggcggt gcagcggctg atggcgcacc ggtcgtttcc
     1801 cgaggccgat gcccgggccc ggatcgcccg acaggcgacc cgcgaggagc gtcgggccgg
     1861 agcgtggatc gtcatcgaca actcgggcga tttcgacgat cttgagcgcc tgatcgacga
     1921 tgcctgggaa cagatcctgg ccaagcgcga cgaggtgctc gccgccggat ttcatggga
//
LOCUS       DAOOMA010000543         1978 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_72413_length_1978_cov_3.645346, whole genome shotgun sequence.
ACCESSION   DAOOMA010000543 DAOOMA010000000
VERSION     DAOOMA010000543.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 1978)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1978)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1978
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_72413_length_1978_cov_3.645346"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..434
                     /locus_tag="PLP95_12275"
     CDS             <1..434
                     /locus_tag="PLP95_12275"
                     /inference="COORDINATES: protein motif:HMM:NF019928.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HPB46624.1"
                     /translation="DTEVVVTRVFDAPPHLVFQAWSTPKLFRLWWVPRSMAMTLHHCE
                     MDVRTGGTYRLNFGGNMDFFGRYLEVTPPSRIVWTNEEGAEEGSVTTVAFEPVDDDKT
                     LLVMSEQFPSKEALDAAGTGAADATHETFAQLDEVLETLGD"
     gene            complement(487..1260)
                     /locus_tag="PLP95_12280"
     CDS             complement(487..1260)
                     /locus_tag="PLP95_12280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HPB46625.1"
                     /translation="MRRRTLALLMAFLTAASAMLTACDLPEQVAGTPCQTEGDLAKDA
                     THVLACTADLRWTTFITLERADLIMTRLAANEPTPASPAPVEATTGCSISRRYAADAA
                     SLPIGQRKHRAIITALGSTRRGCGCLIAREWSIGMEPEAVASLRNAMDQKEALMVAMN
                     PAASIESRTIFVRAAWRSTAEQTCLRRALGRTAEEPGKSFHEFGVAIDLEDWEPRYGD
                     FDRRILQANGWCRTYPAEGWHYEYRPLLEQWGHGSRCID"
     gene            complement(1321..>1978)
                     /locus_tag="PLP95_12285"
     CDS             complement(1321..>1978)
                     /locus_tag="PLP95_12285"
                     /inference="COORDINATES: protein motif:HMM:NF013431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB46626.1"
                     /translation="IPEGRLRYREIDVVLEPTHSDGSAIHGLGADRPWVVIGQSDRSA
                     TLSVVLRTAPYDLHARLSYDLCPDRLQLHLSVTNVGHSVVPVGLGFHPWFRSGMVRLP
                     ATQRWPGEPLPTGPPIAVDGAYDLRGGTIPAAMDACFTGLTDSWADVPGARLRWDDSV
                     SHVVVYSAEPGWLCVEPVTMVNNGIALADRGEAGHGVVALEPGCSMDLRVDIERATSA
                     "
BASE COUNT          364 a          662 c          636 g          316 t
ORIGIN      
        1 cagacacaga ggtcgtcgtc acccgcgtgt tcgacgcccc accgcacctg gtgttccaag
       61 cgtggtcgac gcccaaactc tttcgcctct ggtgggtgcc gcggtcgatg gccatgacct
      121 tgcaccactg cgagatggac gttcggaccg gagggaccta ccgactgaat ttcggaggaa
      181 acatggactt cttcggtcgc tatctcgagg tcacaccacc gtcacgcatc gtgtggacca
      241 acgaagaagg cgccgaggaa ggctccgtca ccactgtggc gttcgaaccg gtcgacgacg
      301 acaagacgct gcttgtgatg agcgagcagt tcccgtcgaa ggaagcgctc gacgccgccg
      361 ggacgggcgc ggccgacgca acccacgaaa ccttcgctca actcgacgag gtcctggaaa
      421 cgctgggcga ctgaacgccc ggcgatgcgc tccctcaccg cggacgggtc gataacgcgc
      481 ctaccgctag tcgatgcagc gcgaaccgtg cccccactgc tccagcagcg gccggtactc
      541 gtaatgccag ccctcggccg gataggtgcg gcaccacccg ttcgcctgga ggatcctgcg
      601 gtcgaaatcg ccgtaccggg gctcccagtc ctcgaggtcg atcgcgacgc cgaactcgtg
      661 aaacgacttg cccggctctt cggcggttcg tcccagcgcg cgtcgcaaac acgtctgttc
      721 ggcggttgaa cgccacgcag cacgcacgaa gatggtgcgc gattcgatcg aggccgcggg
      781 gttcatcgcg accatcaagg cctctttttg gtccatcgcg tttcggaggg aagcgacggc
      841 ctctggctcc ataccgatgc tccactcccg agcgatcaaa cagccacacc ctcggcgggt
      901 cgaccccaac gccgtgatga tggcccggtg cttgcgctgc ccgatgggca gcgacgcagc
      961 atcggccgca taacgacgcg agatcgaaca accggtggtc gcctcgaccg gagcggggct
     1021 cgccggtgtc ggctcgttgg cagcgagccg ggtcatgatg agatccgcac gctcgagcgt
     1081 gatgaaggtc gtccaccgaa ggtccgccgt gcaggcaagt acgtgcgtgg cgtctttggc
     1141 caggtcgccc tcggtctgac acggcgtccc ggccacctgt tcgggcaggt cgcaggcggt
     1201 caacatcgcc gatgccgcgg tcagaaaggc catcaaaaga gcgagggttc ggcgacgcat
     1261 cgttcctcct tgtcgaagtc gcgatcgtat cgccgcgcgc acgccgtgtc ggcgaccgcg
     1321 ctacgccgac gtcgctcgtt cgatgtccac ccgcagatcc attgagcacc cgggctcaag
     1381 cgcgacaacg ccatgaccag cctctcccct gtcggccaac gcaatgccgt tgttgaccat
     1441 cgtgaccggt tcgacgcaca accaacctgg ctctgcgctg tacacgacca cgtgggaaac
     1501 cgaatcgtcc catcgcaggc gcgcaccggg aacatcggcc cacgagtcgg tgagcccggt
     1561 gaagcacgca tccatcgctg cggggatggt cccgccgcgc aggtcgtagg ccccatcgac
     1621 agcgatcggc ggaccggtag ggagcggctc gccaggccat cgttgggtcg caggcaggcg
     1681 aaccatgccg gaacggaacc acgggtggaa cccgaggccg acggggacca cgctgtgtcc
     1741 gacgttggtc accgagagat gtagttgcag tcggtcagga cagaggtcgt agctcaggcg
     1801 agcatgaagg tcgtatggcg ccgttcgcaa gaccacggac aacgtcgcgc ttcgatccga
     1861 ctgaccgatc acgacccagg ggcgatctgc cccgagtcca tggatcgcgc tgccatcgga
     1921 gtgggtcggc tcgaggacga cgtcgatctc gcggtagcgg agccgaccct ccgggatc
//
LOCUS       DAOOMA010000544         1967 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_73054_length_1967_cov_3.514121, whole genome shotgun sequence.
ACCESSION   DAOOMA010000544 DAOOMA010000000
VERSION     DAOOMA010000544.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 1967)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1967)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1967
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_73054_length_1967_cov_3.514121"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            406..894
                     /locus_tag="PLP95_12290"
     CDS             406..894
                     /locus_tag="PLP95_12290"
                     /inference="COORDINATES: protein motif:HMM:NF026766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Imm26 family immunity protein"
                     /protein_id="HPB46627.1"
                     /translation="MGDVLTNLQVLKPSRKKPQAGDLFAMQLPDDRFLFGRVVTTEAM
                     AGPSMGGAILIYVYRPRFDSKEVPERSALSAGELLVSPMMTNRLPWSKGYFETVAHWP
                     IEAGDVLEQHCFRRWDGRYFDEALNEVPGAVEPVGDYGLHSYRTIDDEVSDALGFERA
                     PD"
     gene            complement(1191..>1967)
                     /locus_tag="PLP95_12295"
     CDS             complement(1191..>1967)
                     /locus_tag="PLP95_12295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HPB46628.1"
                     /translation="DIVNKVAIFTVWQANYEVCGVRKMWKAMRRADSDIGRDQVGRLM
                     SELGIAGARRGKRVRTTRPDDSAVRPSDLVNRDFAATAPNQLWVTDLTYVPTWSGMVY
                     VSFITDVFSRTIVGWRVAQNMRTDMVLESIEMARWSRGQRLEGFVCHSDAGSQYTSIR
                     YTERLDEIGATPSIGSVADSYDNALAETINGLYKTEVIRRSGPWRDVDQVELATLAWV
                     HWFNNERLHSALGDIPPAEFEAAWHADQKADTEEVLINRG"
BASE COUNT          343 a          588 c          654 g          382 t
ORIGIN      
        1 caccaccccc acctttgaaa cccaagaaat cctgccaact acttgacgcg cgacagatca
       61 acccacccgc cgtacaaacc gcatgaccac ccacaaccac ccgcaacaaa accggcaaaa
      121 ccttattgac acgctccgct acggagtcag caacggcaag aacgtgtacg tggggattac
      181 gaacaacgtt gctcgtcgtc aggcggagca cgggtcgagg ttcgtgctcg atccgatcac
      241 ttcgtcgccg gtaacgagag ggcaggcacg ggccattgag gaggcgttga tcgtccgaaa
      301 cccgggtttc cagaacgtgc gacacagcat cagtccgaac cattcgtggt accaggaggc
      361 tgtcgactgg ggcgaagctt ggctccgagc caacgggttg tagcgatggg tgacgttctc
      421 acgaaccttc aggtcctgaa gccatcgcgc aagaagccgc aggccggaga tctcttcgcg
      481 atgcagttgc ccgatgaccg gttcctgttc ggtcgggtcg tcacgacgga agcgatggcc
      541 ggaccgtcga tgggtggcgc gattctgatc tatgtctacc ggcctcgctt cgactccaag
      601 gaggtgccgg agcgctcagc gctttcggcc ggtgaactgt tggtgtcgcc gatgatgacg
      661 aaccgcctac cgtggtcgaa ggggtacttc gagacggtgg ctcactggcc gatcgaggcc
      721 ggtgatgtcc tcgagcagca ctgcttccgg cgttgggacg gtcgttactt cgatgaggcg
      781 ctcaacgagg tgccgggcgc ggtggagccg gtcggtgact acgggttgca tagctaccgg
      841 acgatcgacg acgaggtcag tgacgccctg gggtttgagc gggcgcctga ctgaccgtcg
      901 agcccgtcta cgggtcggcc agttctatcg gtcagtgcac ggtgagggca tggtagaggg
      961 ttgagggtga ttggtagtcg agtttgcggc ggcgttgtcc gttgatgatg tcggcggcgt
     1021 gttgggcttg agcgggggtg accagtgaca ggttggtgcc tcgtgggaac cagaactgaa
     1081 tcgccctggg ttcggtagag gctcggtggt ttggttttcg agccggccat caccggcgtg
     1141 gttcacatca tgacgcgtgc gctgcggcaa cgcgaatcct gcgtggggcg tcaaccccgg
     1201 ttgatgagca cctcctccgt gtccgccttc tggtcggcgt gccaggcggc ttcgaactcg
     1261 gccggtggga tatcgccgag ggcgctgtga aggcgctcgt tgttgaacca gtgaacccag
     1321 gccaaggtgg ccagctcgac ttggtcgacg tcgcgccacg ggccgctccg tcggatcacc
     1381 tcggtcttgt agagaccgtt gatcgtctcg gcgagcgcgt tgtcgtagct gtccgcgacc
     1441 gacccgatcg agggggtcgc tccgatctcg tcgaggcgtt cggtgtagcg gatcgacgtg
     1501 tattggctgc cggcgtcgga gtggcacacg aacccctcca ggcgttggcc gcgtgaccag
     1561 cgggccattt cgatcgactc caacaccatg tcggttcgca tgttctgagc gacccgccac
     1621 ccgacgatcg tgcgggagaa cacgtcggtg atgaacgaca catacaccat ccccgaccag
     1681 gtgggcacat acgtcagatc cgtcacccac agctggttcg gggccgtggc ggcgaagtcg
     1741 cggttcacca agtccgatgg tcgcacagcg ctgtcgtcgg gccgggtggt gcggacgcgt
     1801 ttgcctcgcc gggccccggc gataccgagt tcgctcatca gccggccgac ctggtcgcgg
     1861 ccgatatcgc tgtcggcgcg gcgcatcgct ttccacattt tgcggacacc gcagacctcg
     1921 tagttggcct gccacaccgt gaagatcgcg accttgttca cgatgtc
//
LOCUS       DAOOMA010000545         1965 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_73159_length_1965_cov_4.061257, whole genome shotgun sequence.
ACCESSION   DAOOMA010000545 DAOOMA010000000
VERSION     DAOOMA010000545.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 1965)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1965)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1965
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_73159_length_1965_cov_4.061257"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..660
                     /locus_tag="PLP95_12300"
     CDS             <1..660
                     /locus_tag="PLP95_12300"
                     /inference="COORDINATES: protein motif:HMM:NF014042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-phospho-L-lactate transferase CofD family
                     protein"
                     /protein_id="HPB46629.1"
                     /translation="LAEGAALDVVTAEMARAWGLELRIVPVTNDRLSTKLHTEDGRLD
                     FQEYFVREHHEVAVTGVEFDGTDTATAAPGVLGSIAGAEVIVIAPSNPIVSVGPVLAV
                     PGVRDAIEARREVAVAVSPIIGGKALKGPADRLLRELGHESSALGVARMYVDLVSTMV
                     IDTVDADLAPDIEALGMTVVVTDTIMSDLDIARRLALSTVEAITGTASSTPHAASGEP
                     S"
     gene            657..1361
                     /gene="cofE"
                     /locus_tag="PLP95_12305"
     CDS             657..1361
                     /gene="cofE"
                     /locus_tag="PLP95_12305"
                     /EC_number="6.3.2.31"
                     /inference="COORDINATES: protein motif:HMM:TIGR01916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043773 - coenzyme F420-0 gamma-glutamyl
                     ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="coenzyme F420-0:L-glutamate ligase"
                     /protein_id="HPB46630.1"
                     /translation="MSELRIFGVEGIGEIARGTDLASAICAAVELCDGDVIVVTQKIV
                     SKAEGAMVEVDPDDPLSHKPIVERESRRILRRRGELIISETKHGFVCANAGIDLSNVD
                     RGQAALLPDDPDRSARRIRDAIRGKFGVTVGVVVSDTFGRPWRRGVTDVAIGSAGIVA
                     VLDLRGTVDAYGRELMVTEVCVVDELCGAADLVMGKSTGVPVAVVRGVDPSWLGDVGG
                     GVLTDVVRSPADDLFR"
     gene            complement(1375..>1965)
                     /locus_tag="PLP95_12310"
     CDS             complement(1375..>1965)
                     /locus_tag="PLP95_12310"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HPB46631.1"
                     /translation="VAPDAILATNTSSLPVTEAAPQRPESPYGVSKKVFTDYLVAYNT
                     LYGLDFTSLALANVYGPRQDPHGEAGVVSIFAGRLLAGEPCTIFGDGTQTRDFVFVDD
                     VVDAFVRAATRGDGILFNIGTGREVSVNELYTSMATAADVSEPAIYAPARTGELDRIS
                     LSAAKAAVHLGWESWTHLDAGTSAVIEWARGEQSAP"
BASE COUNT          297 a          680 c          665 g          323 t
ORIGIN      
        1 ctcgctgagg gggcggcact cgacgtcgtc accgcggaga tggcccgggc gtgggggctc
       61 gagctgcgaa tcgttccggt cacgaacgac cgtttgtcca ccaagctcca caccgaggac
      121 ggtcgcctgg actttcagga gtatttcgtt cgagagcacc acgaggttgc ggtgaccggg
      181 gtggagttcg acggaaccga caccgcgacc gcggcgcccg gggttctcgg ttccatcgcc
      241 ggggccgagg tcatcgtgat cgcaccgtcg aacccgatcg tgtcggtggg cccggtgctc
      301 gccgtgcccg gggttcgcga cgcgatcgag gcccggcgcg aggttgccgt ggccgtgtca
      361 cccatcatcg ggggcaaggc actcaagggg cccgcagatc gcctgctgcg cgagctcggc
      421 cacgagtcct cagcgctcgg ggtcgcccgc atgtacgtcg acctcgtctc cacgatggtg
      481 atcgacaccg tcgacgccga tctcgccccc gacatcgagg ccctcgggat gaccgtggtg
      541 gtcaccgaca cgatcatgag cgacctcgac atcgctcgcc ggctcgcgct cagcaccgtc
      601 gaagcgatca ccggcactgc atcctcaacc ccccacgcag catcgggaga gccgtcatga
      661 gcgaactgcg aatcttcggc gtcgagggca tcggcgagat cgcccgtgga accgaccttg
      721 cctccgcaat ctgcgccgcc gtcgagttgt gcgacggcga cgtgatcgtg gtgacccaga
      781 agattgtctc gaaggccgag ggagccatgg tcgaggtcga ccccgacgat ccgctcagcc
      841 acaagccgat cgtcgaacgc gaatcgcgtc gaatcctgcg ccgccgcggc gaactcatca
      901 tctccgagac caaacacggg ttcgtctgcg cgaacgccgg catcgacctc tccaacgtcg
      961 accgcggcca ggccgcgctg ttgcccgacg acccggaccg ctcggcgcgg cgcatccgcg
     1021 acgccatccg cggcaagttc ggggtcacgg tcggggtggt cgtgagcgac accttcggac
     1081 gcccgtggcg tcgcggggtc accgacgtcg ccatcggttc cgccggcatc gtcgcggtgc
     1141 tcgacctgcg cggcacggtc gacgcctacg gccgcgagct catggtcacc gaagtctgcg
     1201 tggtcgacga gttgtgcggt gccgccgacc tcgtcatggg caagtcgacc ggcgtgcccg
     1261 tcgccgtcgt ccggggcgtc gatccgagct ggttgggcga cgtcggcgga ggtgttctca
     1321 ccgacgtcgt tcgctccccc gccgacgacc ttttccgtta ggcccaggct cgcatcacgg
     1381 tgcgctctgt tcgccgcggg cccactcgat caccgcgctc gtgccggcgt cgaggtgggt
     1441 ccacgactcc cagcccaggt gcaccgcggc cttggctgcg ctcaacgaga ttcggtcgag
     1501 ttcgccggtt ctcgccggag cgtagatcgc cggttcgctc acgtcggccg ccgtcgccat
     1561 cgaggtgtac aactcgttga ccgacacctc gcggcccgtg ccgatgttga acaggattcc
     1621 gtcgccgcgt gtcgcggctc gcacgaacgc gtcgaccacg tcgtcgacga aaacgaaatc
     1681 tcgggtctgg gtcccgtcgc cgaagatcgt gcacggttca ccggcgagca gtcgcccggc
     1741 gaagatggac accacccctg cctccccgtg cgggtcctgg cgcgggccgt acacgttggc
     1801 caacgccagc gaggtgaagt cgagtccgta gagcgtgttg taggcgacga gatagtcggt
     1861 gaacaccttc ttgctcacgc cgtaaggcga ctcgggccgc tggggcgcag cctcggtcac
     1921 cggcagcgag gaggtgttcg tggcgaggat ggcgtccggg gcgac
//
LOCUS       DAOOMA010000546         1964 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_73258_length_1964_cov_2.336826, whole genome shotgun sequence.
ACCESSION   DAOOMA010000546 DAOOMA010000000
VERSION     DAOOMA010000546.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 1964)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1964)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1964
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_73258_length_1964_cov_2.336826"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1232)
                     /locus_tag="PLP95_12315"
     CDS             complement(<1..1232)
                     /locus_tag="PLP95_12315"
                     /inference="COORDINATES: protein motif:HMM:NF013106.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HPB46632.1"
                     /translation="MAYLYAEQDRQEVELSEVPEELKQAVLGTEDADFYHHNGVSVRA
                     LARAFSANVESGATGQGGSTITQQLAKISLGNTDKTLKRKAQDAVLALRLEEQMTKDQ
                     ILERYLNTVYLGHGAYGVQAAAETYFNASVGDLGWSESILLAGMIQSPYRYDPTREIG
                     HERALKRREVVVGQLRSKGIITAEIAEEVLSTPIATEVFERRASGESDQLVGGGYFSE
                     QVKQQLLSMPELGETRQDRYNAVFSGGLRVYATYDPKAQRMAEEAVKEVPDSKGKYFT
                     GLVSLDPDTSAVRAMVGGPDFETSQFNYVTQAWRQPGSTFKYFVLVAALERGYVPNDI
                     ISGVSPCRFPDPSAPGGIAKISGGGKTASLTSQTQSSSNCGFVRLGQVVGLDIVSEVA
                     DRMGITTIDADFNEVPIT"
     gene            complement(1251..1466)
                     /locus_tag="PLP95_12320"
     CDS             complement(1251..1466)
                     /locus_tag="PLP95_12320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB46633.1"
                     /translation="MDSVDLHPPRRADTRVIAFGLRLLTVFGGGGAAVALVIALIAGV
                     TSNVSSVVPSVNQDEVLLAPLAVRSII"
     gene            1559..>1964
                     /locus_tag="PLP95_12325"
     CDS             1559..>1964
                     /locus_tag="PLP95_12325"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HPB46634.1"
                     /translation="MATPPTTSTIKVALAGAGWAEQVHTLAAAAVPGITIPAVATRSL
                     ESARAFAELVGGEGVGIEELPAAADAVVVATPPTSHADIAVAMLERGVPVLVEKPLAC
                     TLAEADAIVDAAQRNGVAACYAENLLFAPALDL"
BASE COUNT          313 a          656 c          648 g          347 t
ORIGIN      
        1 tcggtgatcg gaacctcgtt gaagtccgcg tcgatcgtgg tgatgcccat gcggtccgcg
       61 acctcggaca cgatgtcgag accgacgacc tgacccagcc ggacgaagcc gcagttcgac
      121 gagctctgcg tctgcgaggt gagcgaggcc gtctttccgc ctccggagat cttcgcgatt
      181 ccgcccgggg cgctcgggtc ggggaaccga cacggcgaaa cccccgagat gatgtcgttc
      241 ggcacgtaac cacgttcgag cgcagcgacg agcacgaagt atttgaacgt cgaacccggc
      301 tgacgccacg cctgggtcac gtagttgaac tgggaggtct cgaaatccgg gccgccgacc
      361 atggcgcgca cggcactggt gtcgggatcg agcgacacca gaccggtgaa gtatttaccc
      421 ttcgagtcgg gcacctcctt caccgcttct tccgccatgc gctgggcctt ggggtcatag
      481 gtcgcgtaga cgcgcaggcc gccgctgaac acggcgttgt atcggtcctg gcgggtctcg
      541 ccgagctcgg gcatcgacag cagttgctgt ttgacctgtt cggagaagta tccgcctccg
      601 acgagttggt cggactcccc cgacgccctg cgctcgaaca cctccgtggc gatcggcgtc
      661 gacagcacct cctcggcgat ctccgcggtg atgatcccct tggaccggag ttggccaacc
      721 acgacctcac gtcgtttgag cgcccgttcg tgaccgatct cgcgggtcgg gtcgtagcgg
      781 tacggcgact ggatcatgcc ggcgagcaga atcgactcgc tccagccgag gtcgcccacg
      841 gacgcgttga aataggtctc cgccgcggcc tgaacgccgt aggccccgtg accgagatac
      901 acggtgttga ggtagcgctc aaggatctgg tccttggtca tctgctcctc gagacgcagg
      961 gccaacacgg cgtcctgcgc cttgcgtttg agcgtcttgt cggtgttgcc gagcgagatc
     1021 ttcgcgagct gctgggtgat cgtcgagccg ccctgcccgg tcgcccccga ttcgacgttc
     1081 gccgagaagg ctcgggccag cgcccgcacg ctgacgccgt tgtggtggta gaagtcggca
     1141 tcctcggttc cgagcacggc ctgcttcagc tcctcgggca cctccgagag ctcgacttcc
     1201 tggcgatcct gttcggcata caggtacgcc atctcgtttc ctgccgagtc ttagatgatc
     1261 gaacgcacgg cgagcggtgc gagcagcacc tcgtcctggt tgacgctcgg cacgacgctc
     1321 gacacgttcg acgtcacccc ggcgatcagc gcgatgacga gcgcgacggc tgcgccgccg
     1381 ccgccgaaaa ccgtcagcag gcggagcccg aatgcgatga cgcgggtgtc agcccgtcgt
     1441 gggggatgta ggtcgacgga gtccactgcg tcgacatgct actttccgcc ctcaaacccg
     1501 ttctgcgata cctgagggtc cacacggcga cccggcgcac cgggctcgga gatcgaccgt
     1561 ggccactccc ccaacgacct cgaccatcaa agtggcgctc gccggtgccg gctgggcgga
     1621 acaggtgcac accctggccg cagccgccgt gccggggatc acgattccgg cggtggcgac
     1681 gcgttcgctc gagtcggcgc gtgcgttcgc cgaactcgtc ggcggcgagg gggtcggcat
     1741 tgaggagctt cccgccgccg ccgatgcggt ggtggtggcc accccgccga cgagccacgc
     1801 ggacatcgcg gtggcgatgc tcgaacgcgg agtgccggta ctggtggaaa agccgctcgc
     1861 ctgcaccctg gccgaagcgg acgccatcgt tgacgccgct cagcgcaacg gcgttgcggc
     1921 ctgttatgca gaaaacttgc tgttcgcacc ggcgctcgat ctcg
//
LOCUS       DAOOMA010000547         1960 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_73444_length_1960_cov_4.247769, whole genome shotgun sequence.
ACCESSION   DAOOMA010000547 DAOOMA010000000
VERSION     DAOOMA010000547.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 1960)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1960)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1960
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_73444_length_1960_cov_4.247769"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1260)
                     /gene="ccrA"
                     /locus_tag="PLP95_12330"
     CDS             complement(<1..1260)
                     /gene="ccrA"
                     /locus_tag="PLP95_12330"
                     /EC_number="1.3.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012224829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043880 - crotonyl-CoA reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crotonyl-CoA carboxylase/reductase"
                     /protein_id="HPB46635.1"
                     /translation="MSMQHILEAIQNGASGEELAALPLPESYRAAYVLRDEQTMWDGV
                     ESEDKDPRQSLHVGEVATPEIAPDEVYIAVMASSINFNTVWTSIFEPLPTFLFLDRLG
                     KESVWGARHAQDFHVVGSDASAVVLRVGSAVRNWKPGDHVTVHCNYLDDQDPSAHNDS
                     MLAANQRIWGFETNYGGLADLSIVKANQLMPKPAHLSWEEAAVNALCASTSYRMLVGD
                     HAGRMKQGDNVFIWGATGGIGAYAAQLVLNGGGTPVGVVSSENRVKLLNAMGVEHAID
                     RREENYQFWSDEHTQDESEWRRLGKKVRSLIGDDPDIVFEHPGRSTMGASVFITKRGG
                     KIVTCAATSGYMLEFDNRHLWMKLKSIISSHFANYQEAWNMNQLISQGRIQPVMSEVF
                     SLDQVGEAALKVHRNQAEGKLGVLCLAP"
     gene            1393..>1960
                     /locus_tag="PLP95_12335"
     CDS             1393..>1960
                     /locus_tag="PLP95_12335"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012224825.1"
                     /note="Catalyzes the synthesis of acetoacetyl coenzyme A
                     from two molecules of acetyl coenzyme A. It can also act
                     as a thiolase, catalyzing the reverse reaction and
                     generating two-carbon units from the four-carbon product
                     of fatty acid oxidation; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HPB46636.1"
                     /translation="MAGSVIISGARTPIGKLSGGLANFSGSDLGGLAIKAALERAGIS
                     GEQVDYVFMGQVLLGGAGQMTARQAAVAAGIPMTVPATTINKVCLSGINSIMMADLLI
                     QSGQADIVVAGGMESMTNAPYLLPGARGGYRMGDKKVIDSMMFDGLTCAIDSLAMGAA
                     TEKYAGVAGLTRDEQDQVAYLSNARAAAA"
BASE COUNT          352 a          639 c          617 g          352 t
ORIGIN      
        1 cggagccagg cacagcacac ccagtttgcc ttcggcctgg ttgcggtgga ctttgagggc
       61 ggcctcgccg acctggtcga gtgagaacac ctcgctcatc acgggctgga tacgaccctg
      121 agagatgagc tggttcatgt tccaggcttc ctggtagttc gcgaaatggc tggagatgat
      181 gctcttcagc ttcatccaca ggtggcggtt gtcgaactcg agcatgtagc cggaagtcgc
      241 cgcgcaggtg acgatcttgc cgccgcgctt ggtgatgaac accgaggcgc ccatcgtcga
      301 gcggcccgga tgctcgaaca cgatgtcggg atcatcgccg atgagcgaac ggaccttctt
      361 gcccaggcgg cgccattcac tttcgtcctg ggtgtgctcg tcgctccaga actggtagtt
      421 ctcctcgcga cggtcgatcg catgctcgac gcccatcgcg ttcaggagct tcacccgatt
      481 ctccgaggac acgacgccaa ccggggttcc cccgccgttg aggacgagct gtgccgcata
      541 ggcaccaata ccgccggtcg ctccccagat gaacacgttg tcgccctgct tcatgcgtcc
      601 ggcgtgatcg ccgacgagca tgcggtagga cgtcgatgcg cacaacgcgt tcaccgccgc
      661 ttcctcccac gacaggtggg ccggcttcgg catgagctga ttggccttca cgatcgacag
      721 gtccgcgagg ccgccgtagt tcgtctcgaa tccccagatg cgctgattcg ccgccagcat
      781 cgaatcgttg tgtgcggacg gatcctggtc gtcgaggtag ttgcagtgca ccgtgacgtg
      841 gtcgcccggt ttccagttgc gcaccgcgga gccaacacgc aacaccactg ccgacgcatc
      901 ggaaccgacg acgtggaagt cctgagcgtg gcgcgcaccc cagaccgatt ccttacccag
      961 ccggtcgagg aagaggaagg tcggcagggg ttcgaagatc gaggtccaca ccgtgttgaa
     1021 gttgatcgac gaggccatga ccgcgatgta cacctcgtcc ggcgcgatct ccggcgtggc
     1081 gacctcaccc acgtgaagcg actgacgggg gtccttatcc tcggactcga cgccgtccca
     1141 catcgtctgt tcgtcgcgca gcacatacgc cgcccgatag gactctggga gaggcagcgc
     1201 ggcgagttcc tcgcccgagg caccgttctg gatggcttcg aggatgtgtt gcatcgacat
     1261 ggcgcaagac gataacgact gacgatacct cttgcccaga ccggacccgg ccgccggtcg
     1321 gcgttggcag aggccggtta ccgaccggta acatcgaccg atcacaacca tccacgacca
     1381 aggggactct atatggccgg atcggtgatt atcagcgggg ctcgtacccc gatcggcaag
     1441 ctctcgggcg ggctcgcgaa cttcagcggc agcgacctcg gcggactcgc catcaaggcc
     1501 gctctcgagc gcgcagggat ctcgggcgaa caggtcgatt acgtcttcat gggccaggtg
     1561 ctcctcggcg gggccggcca gatgaccgcc cgccaggccg ccgtggccgc gggaattccg
     1621 atgacggttc cggcaacgac gatcaacaag gtctgcctgt ccggtatcaa ctcgatcatg
     1681 atggccgacc tgctgatcca gtcaggtcag gccgacatcg tcgtcgccgg cggcatggag
     1741 tcgatgacga atgccccgta cctgcttccc ggcgcccgcg gcgggtaccg catgggcgac
     1801 aagaaggtca tcgactcgat gatgttcgac ggactcacgt gtgccatcga cagcctcgcc
     1861 atgggcgctg ccaccgagaa gtacgccgga gttgccggcc tcacccgcga tgagcaggac
     1921 caggtcgcct acctgtcgaa cgcacgcgct gcggccgctc
//
LOCUS       DAOOMA010000548         1958 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_73544_length_1958_cov_4.475565, whole genome shotgun sequence.
ACCESSION   DAOOMA010000548 DAOOMA010000000
VERSION     DAOOMA010000548.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 1958)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1958)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1958
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_73544_length_1958_cov_4.475565"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..182)
                     /locus_tag="PLP95_12340"
     CDS             complement(<1..182)
                     /locus_tag="PLP95_12340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB46637.1"
                     /translation="MDGFAARCERLVELLREEAPSAEEQRRLSDRVIAAAHEEDLFRS
                     VVPSSLGGLAEPLDELL"
     gene            274..957
                     /locus_tag="PLP95_12345"
     CDS             274..957
                     /locus_tag="PLP95_12345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HPB46638.1"
                     /translation="MRPNAVHHLAIMTRDLPAQLEFFTQVLGLPLVALYDMHGVEGAL
                     HAFVALNDAEYVAFVWAPGVDEIEPIDQVTHAGHGGGLSAAGTMQHVSFNVDTFDALE
                     TLRDRLRSSGVPVFGPIDHGMCSSIYFAGPEGLTLEAAWSAKPVDGREWIDPSVFERA
                     GVSAGDVERFRQPPTFERPAEAVAQPGLDAAGPHLGYPSDQYEFMLTLPDHVIAKGAS
                     VPDPPVRID"
     gene            complement(977..1537)
                     /locus_tag="PLP95_12350"
     CDS             complement(977..1537)
                     /locus_tag="PLP95_12350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012225219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HPB46639.1"
                     /translation="MSGYPREELEAMVDKWLEANRIAEESGDWRSMVDCYADDAHYGW
                     NYGPEVDFMANGKDEIRDWAIGLEMDGLAGWTYPYESLIIDEKKGMFIGLWRQVADVK
                     RDDGSDYDIAGIGGSWFKYAGNMQWAWQRDWFDLGNAASLFIELMGKDQLSEGMTQRM
                     NRRLGPPTPGHYRRGESPRPIWPEDR"
     gene            1625..>1958
                     /locus_tag="PLP95_12355"
     CDS             1625..>1958
                     /locus_tag="PLP95_12355"
                     /inference="COORDINATES: protein motif:HMM:NF013530.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HPB46640.1"
                     /translation="MKVLVTGASGFVGSHTVAALDAAGHEVTAFVRSPQKLATALAPF
                     DLARQPSVAVGDLGSAASIAEAVAAGSHGAAGGFEAIVHAAADVGVAGGSGPAGDINV
                     AGTRAVLEA"
BASE COUNT          295 a          659 c          659 g          345 t
ORIGIN      
        1 agtagttcgt cgagcggttc ggcgagtccg ccgagcgagg agggcaccac cgatcgaaac
       61 agatcctcct catgggcggc ggcgatgacc cgatcgctga gccgacgttg ttcctccgcg
      121 ctgggtgcct cctcgcgaag cagctccacc aggcgctcgc aacgtgcagc aaacccgtcc
      181 atagatcccc cttgattcgg tcggccgtcg tcgggccgcg ccctcggcgc acaaactaag
      241 ttcggccatg ttgtcatctc aagggggaag ctgatgaggc ccaacgccgt ccatcacctg
      301 gcgatcatga ccagggacct gcccgcgcag ctcgagttct tcacccaggt gctcggcctg
      361 ccgctcgtgg ccctctacga catgcacggc gtcgaaggtg cgctacatgc gttcgtggcg
      421 ttgaacgacg cggagtacgt ggcgttcgtc tgggcgccgg gcgtcgacga gatcgagccg
      481 atcgaccagg tgacccacgc cggacacggc ggcgggctga gcgcggcagg aacgatgcaa
      541 cacgtgtcgt tcaacgtcga taccttcgac gcgctcgaga ccctgcgcga ccgactgcga
      601 tccagcggcg tgccggtgtt cggtccgatc gaccacggga tgtgttcgtc gatctacttc
      661 gctggcccgg aggggctgac actcgaggcc gcgtggtcgg ccaagcccgt ggacggccgc
      721 gaatggatcg acccgtcggt gttcgaacgg gccggggtga gcgctggcga cgtcgagcgg
      781 ttccggcagc cgccgacctt cgaacgcccg gccgaggcgg ttgcgcagcc gggcctcgac
      841 gccgccgggc cccacctcgg ctacccgagc gatcagtacg agttcatgtt gacgctgccc
      901 gaccacgtca tcgccaaggg agcgagcgtg cccgaccctc ccgtgcgcat cgactgaacc
      961 gcccggggca cttcgctcag cggtcctcgg gccagatggg gcggggcgac tcgccgcgcc
     1021 ggtagtggcc gggggtgggc gggccgagtc ggcggttcat gcgctgggtc atgccctcgc
     1081 tgagttggtc cttgcccatc aactcgatga acaacgacgc ggcattgccg aggtcgaacc
     1141 agtcgcgctg ccaggcccac tgcatgttgc cggcgtactt gaaccacgat ccgccgatac
     1201 cggcgatgtc gtagtcggag ccgtcgtcac gcttcacgtc cgcgacctga cgccacaggc
     1261 cgatgaacat gcccttcttt tcgtcgatga tcagcgactc atacgggtag gtccagccgg
     1321 ccagcccgtc catttccagc ccgatcgccc agtcgcggat ttcgtctttg ccgttggcca
     1381 tgaagtcgac ctcggggccg tagttccagc cgtagtgcgc atcgtcggcg tagcagtcga
     1441 ccatcgatcg ccaatctccc gactcttcgg cgatacggtt ggcctcgagc cacttgtcga
     1501 ccatcgcttc gagttcttcg cgtggatatc cggacatagt tccccctcgt cgtggtgtcg
     1561 ggtgtcgcag gtggctaggt ttccacctgt aaagttgtct gtcaaccgtg gggaagggtt
     1621 tggaatgaag gttctggtca ccggagcgag cggctttgtc ggcagccata ccgtcgcggc
     1681 cctcgacgcc gccggacacg aggtgaccgc gttcgtgcgg tcgccacaga agctggcgac
     1741 cgcgctggcc ccgttcgatc ttgcccgtca gccgtcggtc gcggtcggcg acctcgggtc
     1801 cgcggcgtcg atcgccgaag ccgtcgccgc cggatcgcac ggggccgccg gcgggttcga
     1861 ggcgatcgtt cacgccgcgg ccgacgtcgg ggtggccggc gggagcggac ctgccggcga
     1921 catcaacgtc gccggcaccc gcgccgtgtt ggaggcgg
//
LOCUS       DAOOMA010000549         1956 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_73684_length_1956_cov_2.695950, whole genome shotgun sequence.
ACCESSION   DAOOMA010000549 DAOOMA010000000
VERSION     DAOOMA010000549.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 1956)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1956)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1956
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_73684_length_1956_cov_2.695950"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..183)
                     /locus_tag="PLP95_12360"
     CDS             complement(<1..183)
                     /locus_tag="PLP95_12360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB46641.1"
                     /translation="MRIANIDGVPHLISNTPNGDHDCISVAAATGGAITAGFPEIFGV
                     LDQVSALLRKVEVGDVL"
     gene            complement(268..918)
                     /locus_tag="PLP95_12365"
     CDS             complement(268..918)
                     /locus_tag="PLP95_12365"
                     /inference="COORDINATES: protein motif:HMM:NF020387.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RES family NAD+ phosphorylase"
                     /protein_id="HPB46642.1"
                     /translation="MIPGRYASDAEHVLGDLADTDADLDSLVRLSAATNSRLLAQEER
                     HPGGLGRGDLVFGVPYSKIVNGAFAYGGQGARFHPPGPRGAWYCALDVATCLAEVAHH
                     RIVHLRETGVTEETDVPYRLFLADIHAQDFALLDDGDSRARSCLDPDSYVGGQALGAR
                     LRSEYRGGVRYPAVRLDGGVCLAVLHAPIVANVRRGDLYMLTISDAALAQVVTVPA"
     gene            complement(987..1427)
                     /locus_tag="PLP95_12370"
     CDS             complement(987..1427)
                     /locus_tag="PLP95_12370"
                     /inference="COORDINATES: protein motif:HMM:NF021255.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2384 domain-containing protein"
                     /protein_id="HPB46643.1"
                     /translation="MTVLDVRLPATPSEVGVWPDLHDRDVRAELTPAAIRAVARVADR
                     WRLTVGDVCSLLGAISESSWHSWQRSAPAALSVDQLTRVSLLLGIYTSLHVLHPGPLA
                     DEWVRRPNSNVLFLGRTPLDVMRVGGIPAMLEVRALLDGRRGGL"
     gene            1558..>1956
                     /locus_tag="PLP95_12375"
     CDS             1558..>1956
                     /locus_tag="PLP95_12375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB46644.1"
                     /translation="MAANFEPTTTPPQSAEFAFFPAEHWERFCETALVQARMAGLDVH
                     FGGPGRLDSSDGIVYSLLPLAKRLADRDIATWDEALEAHFEEMADSLNEPVGPQHLRV
                     RLMAAGSMPPEALDTPFWTEFAPGIWACLVV"
BASE COUNT          365 a          700 c          599 g          292 t
ORIGIN      
        1 caacacgtca ccgacctcga ccttgcgcaa caacgcgctc acctgatcga gcaccccgaa
       61 aatctccggg aaccccgccg tgatcgcgcc gcctgtcgcc gccgcgacac tgatgcagtc
      121 gtggtccccg ttcggcgtgt tgctgatcag gtggggaacc ccgtcgatat tggcgatacg
      181 catgcgacct cctcgcgtct gcgcaatcat cgcaccgatc gcgtgggcga gctccgatgc
      241 gcgccggcga gcgccggtgc gcatacctca ggcggggacc gtcaccacct gcgccaacgc
      301 ggcgtcgctg atcgtcaaca tgtagaggtc cccgcgacgc acgttggcga cgatcggggc
      361 gtgcagcacg gcgagacata ccccaccatc gaggcgcacc gccgggtacc gaacaccccc
      421 gcgatactcc gatcgcagcc gggcaccgag cgcttgacca cccacatagc tgtcgggatc
      481 gagacacgac ctcgcccgcg aatcgccgtc gtcaagcagg gcgaaatcct gggcgtggat
      541 atctgccaga aacagtcgat acggaacgtc ggtctcctcg gtcacccccg tctcgcggag
      601 atgaacgatg cgatgatgcg cgacctccgc cagacacgtg gcgacgtcga gcgcgcaata
      661 ccacgcaccc cgaggccctg gcggatgaaa ccgtgctccc tggcctccgt aggcgaacgc
      721 gccgttgacg atcttggaat agggcacacc gaacacgagg tcgccccgac cgagaccgcc
      781 cgggtggcgc tcctcctggg cgagcagccg actgttcgtc gcggccgaga gccgaacgag
      841 tgaatccagg tcggcgtcag tatcggcaag gtcgccaagg acgtgctccg cgtcgctcgc
      901 gtagcgaccc gggatcaacc gacacgtgtc gtcatgcgca accgcggcca gcggaggcca
      961 ttcgttcgcc gtacgccgct ggccggtcac agtccgccgc gacggccgtc gagcagcgcc
     1021 cggacctcga gcatcgccgg aatacctccg acacgcatca cgtccaacgg ggttcgaccg
     1081 aggaacaaca cattggagtt cgggcgccgc acccactcgt cggcgagcgg ccctggatgc
     1141 aacacgtgca gcgaggtgta gatccccaag agcaacgaga cgcgcgtgag ctgatccacg
     1201 ctcagcgccg caggcgcgga gcgttgccac gagtgccaac tgctctccga gatagcaccg
     1261 aggagactgc acacgtcacc aacggtgagc ctccagcgat ccgccacgcg agccaccgca
     1321 cgaatcgccg ccggggtgag ttcggcacgg acgtcgcggt catgcaggtc gggccagaca
     1381 ccgacctcgc tcggcgtcgc aggcaatctg acatccaaaa ccgtcattcg catcacctcc
     1441 agttctatag gcatagtact cctgaactgg agattctccc cgctcgtcgt cgatcctcgg
     1501 caaccgcact cgggctaccg acccgcacca aactgcggcg acggtagggt tcgggcgatg
     1561 gccgcgaact tcgaacccac cactacccca ccccagagcg ccgagtttgc gttctttccg
     1621 gccgagcact gggagcggtt ctgcgaaacg gcgctcgttc aggcccgaat ggccggcctc
     1681 gatgtccact tcggcggtcc cgggcggctc gattccagcg acggcatcgt ctacagcctg
     1741 ttgccgctcg caaagcgact cgccgaccgc gacatcgcca cgtgggatga ggcgctggaa
     1801 gcccacttcg aggagatggc cgactcgctg aacgagccgg taggtccgca acacctccgc
     1861 gttcgactga tggccgccgg ttccatgccg cccgaggcac tcgacacccc gttctggacc
     1921 gagttcgcac cgggcatctg ggcgtgtctc gtggtc
//
LOCUS       DAOOMA010000550         1955 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_73718_length_1955_cov_4.288421, whole genome shotgun sequence.
ACCESSION   DAOOMA010000550 DAOOMA010000000
VERSION     DAOOMA010000550.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 1955)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1955)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1955
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_73718_length_1955_cov_4.288421"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..386)
                     /locus_tag="PLP95_12380"
     CDS             complement(<1..386)
                     /locus_tag="PLP95_12380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB46645.1"
                     /translation="MTTPKPKAKPRKVPAGKGAVPSRSVVKRPDSLREVVEVSLDDVA
                     DRSLAPKTIELIETMVRDVIEHDRGAEADEWERLVGAVLTPKVTCELLGISRQALDKR
                     RKGAKVLAVPTVANRWVYPLATVGTA"
     gene            complement(863..1555)
                     /locus_tag="PLP95_12385"
     CDS             complement(863..1555)
                     /locus_tag="PLP95_12385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB46646.1"
                     /translation="MNDFNESNADASWTDNSHVGECFGSGQHDVEAWDVDAILDIDID
                     DPEVEAVALALRNEVADWVAQAPLTLVNDSIANAFHQTAGNLGLRDQAIAWALEVRSN
                     LREGERAAIAGNVESIAALLAVCATDPRPSVRAAISVNRLCVDARVQWALTRDDDPLV
                     VLSFLENAEPGREQIEWLIRHSEWDTVRYQIARRRLATDLLELLTDDSNQRVAALATK
                     RLADRLAKRSAR"
     gene            1681..>1955
                     /locus_tag="PLP95_12390"
     CDS             1681..>1955
                     /locus_tag="PLP95_12390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB46647.1"
                     /translation="MVANDGESPTSTPGSHPSTSELKGYGRAGLDRVAAGEWHRAGID
                     HYAAEWHRSEGRSLEDAVRVQTAEQPPNAERRASLSTFDELTGDVWVS"
BASE COUNT          344 a          614 c          626 g          371 t
ORIGIN      
        1 cttgcggtgc caaccgttgc gagggggtac acccaacggt tcgcaacggt tggcaccgca
       61 agcaccttcg cgcctttgcg tcgcttgtcc aacgcctggc gggagatacc caacagctca
      121 catgtgacct tcggggtgag cactgcgcca acgaggcgtt cccactcgtc tgcctctgcg
      181 ccccggtcat gctcgatcac gtcgcgcacc atcgtctcga tgagttcgat cgtcttcgga
      241 gccaggctgc gatcggcgac gtcgtcgagt gaaacctcga cgacttcacg aagcgagtcc
      301 ggtcgcttga cgaccgagcg cgaaggaacg gcgcccttgc ccgctggaac cttcctcggc
      361 ttggccttcg gcttcggtgt cgtcattgca accatgccaa ccagtatgcc gaagtagttg
      421 caggtacggt aggcgcagtc aggtggcggc ccgtggcgcc acgtcgacca cctcgacgtg
      481 gcgcccgtga tggaggggca cgattcggta cccagtggtc ggagctgcac agctgatcgc
      541 cgatctcatg acctgacgga ctgctttcta cggagtcggg agttcgtaga aagcgttgac
      601 cggcgggctg ctcggcgacc cacttgttcg agcggtggag gaggagcggc aagctcacga
      661 tgccttcgaa cttgtcgagt tcaagctgct ccactcgtcg cagatactcc tcgaggcgaa
      721 atgtagtgaa gaccctcgat tcccacctgc cgctccagat gcgcaatccg gaactgtcga
      781 tccacgctcg ggcggcgcat tgagctcgat cggtggtgtc tcgaccggcg gtcgaggaag
      841 agccgcacgc tcaggtcgtc ggtcatcgtg cgctcctctt cgcgaggcgg tcggccagac
      901 gtttcgtcgc aagtgctgcg actcgctgat tcgaatcgtc ggtcaacagc tcgaggaggt
      961 cggtggcgag tcgacgcctg gcgatctggt atcgcacggt gtcccactcg ctgtggcgaa
     1021 tgagccactc gatctgctct cggcctggtt cagcgttctc gaggaacgac agcaccacga
     1081 gcgggtcgtc gtcgcgggtc agcgcccatt ggactctggc gtcgacgcac aggcggttca
     1141 cgctgatcgc ggcgcgaacg ctgggccgag ggtcggtggc gcacacggcg agcagcgccg
     1201 caatggactc gacgttgccg gcaatggccg cccgttcgcc ttcccgcagg ttggagcgga
     1261 cctccaacgc ccaggcgatg gcctgatcgc ggaggccgag gttgcccgct gtctggtgga
     1321 acgcgttcgc gatgctgtcg ttgaccaggg tcagcggcgc ctgcgccacc caatcggcga
     1381 cctcgttgcg caaggcgaga gcgaccgcct cgacctccgg atcgtcgata tcgatgtcga
     1441 ggattgcgtc gacgtcccat gcctccacgt cgtgttgacc gcttccgaag cactccccga
     1501 cgtgtgaatt gtcggtccat gaggcgtcgg cgttcgattc gttgaagtcg ttcatgggat
     1561 ccccagtcgt tgatgtcgtg attcggtggt gcagttccgg tcggcctgca aggagcactg
     1621 aaacagcaca actcagttgt gtcaatacac aaaagtgaga tgaaccgata aattgttgag
     1681 atggttgcaa acgacggcga atcacccaca tccacccctg gttcgcaccc gtcaacctcc
     1741 gaactcaagg ggtacggcag ggcaggcctc gaccgcgtcg ccgcaggtga atggcaccgc
     1801 gccggcatcg accactacgc cgcagaatgg catcgctccg aagggcgttc gctcgaggat
     1861 gcggtccgag tacaaacggc cgagcaaccg ccgaacgccg agcggcgagc gtccttgtcg
     1921 accttcgacg agttgaccgg cgacgtgtgg gtctc
//
LOCUS       DAOOMA010000551         1955 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_73722_length_1955_cov_3.774211, whole genome shotgun sequence.
ACCESSION   DAOOMA010000551 DAOOMA010000000
VERSION     DAOOMA010000551.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 1955)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1955)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1955
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_73722_length_1955_cov_3.774211"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(65..490)
                     /locus_tag="PLP95_12395"
     CDS             complement(65..490)
                     /locus_tag="PLP95_12395"
                     /inference="COORDINATES: protein motif:HMM:NF014660.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliW"
                     /protein_id="HPB46648.1"
                     /translation="MSVLVRSERFGDVECDDAATVVFPDGLLGFEEEREFVVMPADPE
                     GIYSWLQSTRTPSLAFLTTSPTCFFADYAVEIDDAEVAEIEILDESEAMVLAIVTIAE
                     DRATANLLGPIVVNTRTRRARQVVLADSTWSTKELLGEF"
     gene            complement(495..>1955)
                     /locus_tag="PLP95_12400"
     CDS             complement(495..>1955)
                     /locus_tag="PLP95_12400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB46649.1"
                     /translation="EPALSDPVAAGVGGPDPANPGRHLCYEGFPLESALARVSADHGA
                     AAIIVSAERVRRGGVRGFFAREVFQVVVAVPDDAEIDPEDVDGALGLELGESAEFGDD
                     LVEEDSEPIDLTVGAFATDQRVGGPQDDELWALLAAPLPRDPEPRAADRRGQPGSTVV
                     PPPYPGAPGTPTDPHLIRLAPPPPRWPSTHQEPLVDAVVEPVVEAGVASLPFVVDVVE
                     SEAILAELVQPLKSLGPIASTTLVAGPARRSGVESIAEPRAEVVRAEAVRHAKAAFRA
                     SEPSRPVVSSRAGGPEVDLSAMLGALETSLVAVPAPPAAGVIALVGDLAEIITAAQSL
                     ARHLGVAAHDVHVVARGASASPVLGVVRPRSDAARNPQNRTLVAIELAAGRDGHEWTR
                     SALAALDPRQIRFVADARRYLSHQHFSVAAVGGVDVVDLSGVGALAEPADALALGVPV
                     ATIDGRGATPEMWAAVLLAHRCSQRQGTTWSGAEDE"
BASE COUNT          341 a          677 c          650 g          287 t
ORIGIN      
        1 ccgtgatcgt gatgttgccg ttgacgatga tcgattcgtt cgtccgacgt gacagaacca
       61 gcatctagaa ctcgcccaac agttccttgg tcgaccaggt ggagtcggcg aggacgacct
      121 ggcgagcccg gcgcgtccgg gtgttgacca caatagggcc gagcaggttg gcagtggctc
      181 gatcctcggc gatcgtcacg atcgccagca ccatcgcctc ggactcatcc aggatctcga
      241 tctccgccac ctccgcgtcg tcgatctcga ccgcatagtc ggcgaagaag cacgtcggag
      301 acgtcgtcag gaatgccaac gacggcgtcc gggtcgactg caaccagctg tagataccct
      361 ccggatcggc gggcatcacc acgaactcgc gttcttcctc gaagccgagt aggccatcgg
      421 ggaacacgac ggttgcagca tcgtcacact cgacatcgcc gaaccgttct gaccggacca
      481 acacactcat gacactactc atcctccgca ccactccagg tggtgccttg tcgttgtgaa
      541 cagcgatggg ccagcaacac tgccgcccac atttcggggg ttgccccacg cccgtcgatg
      601 gtcgcgaccg gcacaccaag tgccagagca tcggcgggtt cggcaagcgc cccaacccca
      661 ctcaggtcga ccacgtcgac ccctcccacc gcggcaacgc tgaaatgctg atgggaaagg
      721 taccgacgag cgtcggcgac gaatcgaatc tgacgaggat cgagggctgc caacgccgag
      781 cgcgtccact cgtgcccgtc gcggccggcc gccagttcga tggcgacgag ggtccggttc
      841 tgcgggtttc gtgccgcgtc ggagcgtggc cggaccacgc cgaggaccgg cgaagccgac
      901 gcaccgcgag cgacaacgtg cacatcgtgg gccgccaccc ccagatgccg cgccaggctc
      961 tgcgcagcgg tgatgatttc cgcgaggtcg ccgacgagcg ctatgacccc ggccgcaggc
     1021 ggtgcgggaa ccgctacgag tgaggtctcg agggctccca gcatggccga caggtccacc
     1081 tcgggcccgc cggcacggga cgaaacgact gggcgtgacg gttcactcgc ccgaaacgct
     1141 gctttggcat gacgcaccgc ttcggcgcgt acgacctcgg cgcggggttc ggcgatcgac
     1201 tcgactcccg accggcgggc cgggccggcg acgagcgtcg tcgaggcgat cggtccgagc
     1261 gacttcagcg gttgcaccag ttcggcgagg atcgcttcgg attcgacgac gtcgacgacg
     1321 aagggcagcg aggccacccc cgcctcgacc accggttcaa ccacggcgtc gaccagcggt
     1381 tcctggtggg tcgacggcca gcgaggcggc ggcggcgcga gtcggatcag gtgtgggtcg
     1441 gttggggtgc cgggcgcacc cggatacggc ggggggacga cggtggagcc cggctgccct
     1501 cgacgatccg cagcgcgagg ctctgggtcc cgcggcaacg gcgccgcgag caacgcccac
     1561 agctcgtcgt cctgcggacc accgacgcgc tgatcggtcg cgaaggctcc cacggtgagg
     1621 tcgatcggct cggagtcctc ctcgacgaga tcgtcgccga actcagccga ttccccgagt
     1681 tcgagaccca gcgcaccgtc gacgtcctcg ggatcgatct ccgcgtcgtc gggcacggcg
     1741 acgaccacct ggaacacttc gcgggcgaag aagccacgaa cgccgccgcg ccgcacgcgt
     1801 tcggccgaca cgatgatcgc cgcggccccg tgatcggcac tgaccctggc cagcgcggat
     1861 tcgagcggga acccctcgta acacaggtga cgtccagggt tggcagggtc gggtccgccg
     1921 acccctgcag cgactgggtc cgacagcgct ggttc
//
LOCUS       DAOOMA010000552         1950 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_74026_length_1950_cov_2.494459, whole genome shotgun sequence.
ACCESSION   DAOOMA010000552 DAOOMA010000000
VERSION     DAOOMA010000552.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 1950)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1950)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1950
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_74026_length_1950_cov_2.494459"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..368)
                     /locus_tag="PLP95_12405"
     CDS             complement(<1..368)
                     /locus_tag="PLP95_12405"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HPB46650.1"
                     /translation="MTATVSTLTKGEQTRQTILDAAIVRFGRDGYRASSVADIARDAG
                     VGGTVAYAHFENKESLFLAALDTDAAAVINEGVLHLLDEDRIEWQETLVLALIDAIEQ
                     HPLAKRVLSGLEPHATDRVI"
     gene            442..1527
                     /locus_tag="PLP95_12410"
     CDS             442..1527
                     /locus_tag="PLP95_12410"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021623.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HPB46651.1"
                     /translation="MDNSLPPLGGPATSISSAHLAEQLHPSDPNPPGAEMSLSTDTNL
                     DTDLAADGQPADGPAASATGATAATAPLVRQTSNADHDIPVRRVSFEESLRTIPRHYA
                     RDENIFLSHLGVAMSGVFPDGEDFFVKSVRHFRSEITDPVLKKQVAGFIGQESIHGRE
                     HRVLNRRFDELGYKATRIEEITKRGLAFRWKVSKPISCLAITAALEHFTATLAEELLS
                     SDEFRDMFGHPAVKEVFMWHALEEAEHKAVAFDVYRAVGGSERLRIMSMRFIRVAFIL
                     SSTITMLLGVLRDPASRDLRRLRRDWKAFRRSPIVSARLWKILKEYDRRGFHPDDRDT
                     NHLTVEWREKLFGDDGSLNHVLPAKAA"
     gene            1580..>1950
                     /locus_tag="PLP95_12415"
     CDS             1580..>1950
                     /locus_tag="PLP95_12415"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HPB46652.1"
                     /translation="MSNLADDTVADTIDITDAARDEGQPGGVEHLDVLVVGAGLSGIG
                     MACHLVREAPWASFAVFEARDDLGGTWDLFRYPGIRSDSDMFTLGYPFRPWNRKESIA
                     SGGHILEYLRETVEENHLTNL"
BASE COUNT          360 a          650 c          602 g          338 t
ORIGIN      
        1 tcgatgaccc gatcggtcgc gtggggttcg agtccggaca gcacccgctt cgcaagtggg
       61 tgctgttcga tggcgtcgat cagcgcgagc accagggttt cctgccattc gatgcggtcc
      121 tcgtcgagca ggtgcaacac gccctcgttg atgaccgccg cggcgtcggt gtcgagcgca
      181 gcgaggaaaa gcgattcctt gttctcgaag tgggcgtagg cgaccgtgcc gccgacgccg
      241 gcgtcgcggg cgatgtcggc caccgacgac gcgcggtatc cgtcgcgtcc gaaacgcacg
      301 attgccgcat cgagaatcgt ctgacgggtc tgctcgccct tggtgagcgt cgagacggtc
      361 gcagtcatac gaatcagggt agcaccaccc atcaataatt gagggattga aagaatcact
      421 caaatcctct aaggtgttcc cgtggacaat agcttgccgc ctctgggcgg acccgccacg
      481 tcgatctcct cggcacacct cgccgaacaa ctgcacccat ctgatccgaa tcccccagga
      541 gccgaaatgt cgctcagcac cgacaccaac ctcgacaccg atctcgccgc cgacgggcaa
      601 cccgccgacg gccccgccgc ctcggcaacc ggagcgaccg ccgccaccgc tccgctggtt
      661 cgccagacca gcaacgccga tcacgacatt cccgttcggc gcgtgtcgtt cgaggagagc
      721 ctgcgcacca tccctcgcca ctatgcgcgc gacgagaaca tcttcttgtc gcacctgggg
      781 gtcgccatgt ccggcgtgtt tcccgacggt gaggacttct tcgtgaagtc ggtgcgccac
      841 ttccgatcgg agatcaccga tccggtactc aagaagcagg tggcgggctt catcggccag
      901 gagtccatcc acggccgtga acatcgggtg ctgaatcgcc gcttcgatga actcggctac
      961 aaggcgaccc ggatcgagga aatcaccaag cgtgggctcg cgttccgctg gaaggtgtcc
     1021 aagccgatct cgtgtctcgc catcaccgca gcgctcgagc acttcaccgc gacgctcgcg
     1081 gaggagttgc tgtcgagcga cgaattccgc gacatgttcg ggcatccagc ggtgaaggag
     1141 gtgttcatgt ggcatgcgct tgaggaggcc gagcacaagg cggtcgcgtt cgatgtctac
     1201 cgcgccgtcg gcggcagcga gcgcctgcgc atcatgtcga tgcgcttcat ccgggtggcg
     1261 ttcatcctgt cgtcgacgat cacgatgctg ctcggggtcc tgcgagatcc ggcgtcgcgc
     1321 gacctgcggc gtctgcgccg cgactggaag gcgttcaggc gctcgccgat cgtcagtgcg
     1381 aggctgtgga agatcctcaa ggaatacgac cgccgagggt tccatcccga tgatcgagac
     1441 accaaccacc tgacggtcga atggcgcgag aagctcttcg gcgacgatgg ctcgctgaac
     1501 cacgtcctgc ccgccaaggc cgcctgagca gtccctccac gatcgaccga gcaccacacg
     1561 tagaaacgag gacccaacga tgagcaacct cgcagacgac accgtcgcag acacgatcga
     1621 catcaccgac gccgcacgtg acgaaggcca gcccggtggg gtcgaacatc tcgacgtcct
     1681 cgtggtgggc gccggcctgt ccggcatcgg catggcctgc cacctcgtgc gcgaggcgcc
     1741 gtgggcatcg ttcgccgtgt tcgaggcgcg cgacgatctc ggcggaacct gggatctgtt
     1801 tcgctatccg ggcatccggt ccgactccga catgttcacc ctcgggtacc cgtttcgacc
     1861 gtggaatcgc aaggagtcga tcgcatctgg aggccacatc ctcgagtacc tgcgcgagac
     1921 ggtcgaggag aaccacctga ccaacctcat
//
LOCUS       DAOOMA010000553         1945 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_74289_length_1945_cov_4.173016, whole genome shotgun sequence.
ACCESSION   DAOOMA010000553 DAOOMA010000000
VERSION     DAOOMA010000553.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 1945)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1945)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1945
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_74289_length_1945_cov_4.173016"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(63..896)
                     /locus_tag="PLP95_12420"
     CDS             complement(63..896)
                     /locus_tag="PLP95_12420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HPB46653.1"
                     /translation="MQEFRNKVAAITGAGSGIGRALALDLAKRGTHLALSDIDEAGLA
                     ETVALCEGFGAKVSSTRLDVADRDAVFAWAHAVAEEFGTVNLIFNNAGVALGADISEM
                     SIEDFEWLMSINFWGVVHGTQAFLPHLKASGDGHVVNTSSVFGLFSVPSQSAYNAAKF
                     AVRGFTDALRMELEIEGAPVSATTIHPGGIKTNIAKNSRMSDSAAAVAGGRDEVHKQF
                     TKIAMTKPDKAARQIITAVERNRRRALIGPDAKGFDLMSRLPAGLYQSAIVRGARKRL
                     G"
     gene            complement(911..>1945)
                     /locus_tag="PLP95_12425"
     CDS             complement(911..>1945)
                     /locus_tag="PLP95_12425"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HPB46654.1"
                     /translation="FGGQIVHPQFWPEDLDYAGKQVVVIGSGATAVTLVPSMADTAAK
                     VTMLQRSPTYIGSLPAVNPLTATVRKVTPKRAHGTILRWMNSTGTQSFYRLSRRHPEL
                     VKKMLLKRIAQALPDHDVAKHFTPKYNPWDQRFCVVPDGDLFKALRKGTADVVTDHIE
                     RFDETGIVLESGEHLDADIIVTATGLDLLFIGGVELSIDGEAVDIANTMSYKGMGLEG
                     VPNFAVIIGYTNASWTLKADLTAGYVTKLLGFMRERGFTRAMPSKHPELHSDDALLGL
                     DAGYVLRTAGAFPKQGSREPWRLHQSYLKDYRNLKMSGVQDDTMVFSTCTPSAVATSE
                     PVGAVASGAS"
BASE COUNT          319 a          649 c          617 g          360 t
ORIGIN      
        1 gcgcccacaa gttagcgccg acacctcggc gcgccgggtg cccgaaggcg caaccagcga
       61 cctcagccga ggcgcttgcg cgcacctcgc acgatcgccg actgatacag cccggcgggc
      121 aggcgactca tcaggtcgaa gcccttggcg tcggggccga tgagcgcgcg tcgccggttg
      181 cgttcgaccg cggtgatgat ctgacgcgcc gccttgtcgg gcttggtcat cgcgatcttg
      241 gtgaactgct tgtgcacctc gtcgcgtcca ccggccaccg ctgccgccga gtcgctcatg
      301 cgggagttct tggcgatgtt ggtcttgata ccgcctgggt ggatcgtggt cgccgacacc
      361 ggcgcaccct cgatctccag ttccatacgg agcgcgtcgg tgaagccgcg caccgcgaac
      421 tttgccgcgt tgtaggccga ctggctgggc acgctgaaca ggccgaacac cgacgaggtg
      481 ttgacgacgt ggccgtcgcc ggatgccttg aggtgcggta ggaacgcctg ggttccgtga
      541 acgacccccc agaagttgat actcatcaac cactcgaagt cctcgatcga catctcggag
      601 atgtcggcgc cgagcgcaac gccggcgttg ttgaagatga ggttgaccgt gccgaactcc
      661 tcggccacgg cgtgtgccca cgcaaatacc gcgtcgcgat ccgcgacatc gaggcgggtc
      721 gaactcacct tggctccgaa tccctcgcac agcgcgacgg tttcggccag tccggcctcg
      781 tcgatgtccg acagcgccag atgcgtgcca cgcttggcaa ggtcgagggc caacgctcgt
      841 ccgattcccg accctgcccc ggtgatcgcg gccaccttgt tgcgaaactc ctgcatgatt
      901 tcctttcgag ttacgacgcg cccgacgcta cggcgccgac cggctcggac gtggcgaccg
      961 ccgacggcgt acaggtcgaa aacaccatcg tgtcatcctg aactccgctc atcttcagat
     1021 tccggtagtc cttcaggtag ctctggtgca gccgccacgg ctcgcgtgag ccttgcttgg
     1081 ggaacgctcc ggcggtccgc aacacgtatc cggcgtcgag gcccaacaac gcatcgtcgc
     1141 tgtgcagttc cgggtgtttg gacggcatcg cccgggtgaa tccccgctcg cgcatgaagc
     1201 ccaacaactt ggtgacgtat ccggcggtga ggtcggcctt cagggtccaa ctggcgttgg
     1261 tgtagccgat gatcaccgcg aagttcggca ccccttcgag ccccattccc ttgtaggaca
     1321 ttgtgttcgc aatgtcgacc gcctcgccgt cgatgctcag ttcgaccccg ccgatgaaca
     1381 acaggtcgag gccggtcgcc gtcacgatga tgtcggcgtc gaggtgctcg ccggactcca
     1441 acacgatgcc cgtctcgtcg aaccgctcga tgtggtcggt caccacgtcc gcagttccct
     1501 tgcgcaacgc cttgaacagg tcgccgtcgg gcaccacaca gaatcgctga tcccacgggt
     1561 tgtacttcgg cgtgaagtgc ttggcgacat cgtggtccgg aagcgcctgg gcgatgcgct
     1621 tgaggagcat cttcttcacc agttccggat ggcggcggct gagccggtag aacgactggg
     1681 ttcccgtgga attcatccac cgcaggatcg tgccgtgggc gcgcttgggg gtgaccttgc
     1741 gcacggtggc ggtgagcggg ttcaccgccg gcaacgaccc gatgtaggtg ggcgaacgct
     1801 gcagcatcgt cacctttgcg gcggtgtcgg ccatcgacgg caccagggtc accgccgtcg
     1861 cccccgaccc gatgacgacg acctgcttgc ccgcataatc gaggtcctcg ggccagaact
     1921 gcggatgcac gatctgcccg ccgaa
//
LOCUS       DAOOMA010000554         1937 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_74749_length_1937_cov_3.091392, whole genome shotgun sequence.
ACCESSION   DAOOMA010000554 DAOOMA010000000
VERSION     DAOOMA010000554.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 1937)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1937)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1937
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_74749_length_1937_cov_3.091392"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..470
                     /locus_tag="PLP95_12430"
     CDS             <1..470
                     /locus_tag="PLP95_12430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB46655.1"
                     /translation="LGKGPSLSADKAGNGQVAGAAFYMTSSGTDAVVSGAWDFIKYFN
                     DTPQQVRWTREGSYMPTRAAAVTELEADPAWADSQRGTWINTAYESMKDLDASFPGPL
                     VGPYLKVRATVQSMLETIALPDSGGAADRVAPALDEAVAQITSDLANYNSTTR"
     gene            complement(519..1814)
                     /locus_tag="PLP95_12435"
     CDS             complement(519..1814)
                     /locus_tag="PLP95_12435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012223447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HPB46656.1"
                     /translation="MASMLDLDDVRAQVLGDSRGVLGRGLGRSYGDGAQNAGGTVVDG
                     RSAIGIIDATPDGMVTAKAGTSLEELMEWFIPRGWFVPVTPGTRQVTVGGAIAADIHG
                     KNHHRAGSWCNHVHSFRIMVGSGDIVDVSPDSDPDLFWATAGGMGLTGLIIDATFQMK
                     PIETSRLLVDTDRAADLDSVMQLMVEGDHEYDYSVAWIDIMTGGDAMGRSVLDRGRFA
                     RRDELPSKLASDPLSFTSNTLAAFPPIAPSGLINKLTVKAFNELWFRKAPAQRRNGLQ
                     TITQFFHPLDLVSDWNRVYGPKGFLQWQYVVPDEAGEFVRRSLEELSRSGVAPSFLAV
                     LKRMGPGNLGDLSFPAAGWTLAFDAPVGNPELNVILDRLDTEVVEAGGRIYLAKDSRL
                     DPAHMEKMYPRLRHFRETKARVDPQNRFQSDLGRRLGLC"
BASE COUNT          343 a          671 c          613 g          310 t
ORIGIN      
        1 ggttgggcaa gggcccatcg ctgagcgcag acaaggcggg caacggccag gtcgccggtg
       61 ccgcgttcta catgaccagc tccggcaccg acgcggtcgt gtcgggtgcg tgggatttca
      121 tcaagtactt caacgacacc ccacagcagg tgcgttggac acgtgagggc tcctacatgc
      181 cgacccgggc cgccgccgtc accgaactgg aagccgatcc ggcctgggcc gacagccaac
      241 gcggaacgtg gatcaacacg gcctatgagt cgatgaagga cctcgacgcc agcttccccg
      301 gccccctcgt cggcccgtac ctgaaggtgc gcgccacggt gcagtcgatg ctcgaaacca
      361 tcgcacttcc cgactccggc ggcgcagccg atcgggtcgc tcccgccctc gacgaggcgg
      421 tcgcccagat cacctccgat ctggccaact acaacagcac cacccgctga cccgcacgac
      481 ccgctggtag tgacgcgacc ctcgggcggc gtctcggctc aacagagtcc gagacgtcgc
      541 ccgaggtcgc tctggaaacg attctgcgga tcgacccgcg ccttggtctc acggaagtgc
      601 cgcaggcgcg ggtacatctt ttccatgtgc gccggatcga ggcgactgtc cttggccaga
      661 tagatccggc cgccggcctc gaccacttcg gtgtcgagac gatcgaggat gacgttgagc
      721 tccggattac ccacgggggc atcgaacgcg agggtccacc cagcggccgg aaacgacagg
      781 tcaccgagat ttccgggacc catccgtttg agcaccgcca gaaacgacgg tgccaccccg
      841 gaccggctga gttcttcaag gctgcggcgt acgaactccc ccgcctcgtc gggcaccacg
      901 tattgccact gcaggaaccc tttgggccca tacacccggt tccagtcgct gacgagatcg
      961 agcgggtgga agaactgggt gatggtctgc agcccgtttc gacgttgtgc cggagccttg
     1021 cgaaaccaca gctcgttgaa cgccttgacc gtcaacttgt tgatcagccc cgacggggca
     1081 atcggcggaa acgcggcgag cgtgttcgac gtgaaactca gcgggtcgga cgcgagtttc
     1141 gacggcagct cgtcgcgccg ggcgaaccga ccgcggtcca acaccgagcg gcccatcgcg
     1201 tcgccgccgg tcatgatgtc gatccacgcc accgagtagt cgtactcgtg gtccccttcg
     1261 accatgagct gcatcaccga gtcgagatcc gcggctcggt cggtgtcgac gagcagccga
     1321 ctcgtctcga tcggcttcat ctggaaggtg gcgtcgatga tcagcccggt gagccccatt
     1381 cctccggcgg tggcccagaa caggtcgggg tcggaatcgg gggagacatc gacgatgtcg
     1441 ccgctgccca ccatgatccg aaacgaatgg acgtggttgc accacgaacc ggcacgatga
     1501 tggttctttc cgtggatgtc ggcggcgatc gctccaccga cggtcacctg ccgggtcccc
     1561 ggggtgaccg gcacgaacca gccgcgcgga ataaaccact ccatcagctc ctcgagcgag
     1621 gtgccggcct tggcggtcac catcccgtcg ggggtggcgt cgatgatgcc gatcgcgctg
     1681 cggccgtcga ccacggtgcc tccggcgttc tgcgctccgt cgccgtagga ccgtcccaga
     1741 ccccgaccga gcacaccgcg cgaatcgccg agcacctggg cgcgaacgtc gtcgaggtcg
     1801 agcatcgagg ccatgtgtga tgccgtcggc gccgttcgac cccacccttg caacaaccgg
     1861 gtcggatggg tccgcgcgcc acctgaccgc gcgccacctg accgagcgtc acctgacggc
     1921 tccgtcatcg gatcaac
//
LOCUS       DAOOMA010000555         1936 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_74801_length_1936_cov_3.374801, whole genome shotgun sequence.
ACCESSION   DAOOMA010000555 DAOOMA010000000
VERSION     DAOOMA010000555.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 1936)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1936)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1936
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_74801_length_1936_cov_3.374801"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..242
                     /locus_tag="PLP95_12440"
     CDS             <1..242
                     /locus_tag="PLP95_12440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB46657.1"
                     /translation="SLTLDVKHAEAEKIFDAHFTASTDNGYPVTTSNHPELTSTGPAK
                     YSVLPGTLSSWAPIHPAAGGPADFLTSWCCLSLRR"
     gene            215..820
                     /locus_tag="PLP95_12445"
     CDS             215..820
                     /locus_tag="PLP95_12445"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF014453.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006486 - protein glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HPB46658.1"
                     /translation="MVLLIAAPVIVPVGVLCALAIRLDSSGPALFRQERVGKNGSPFT
                     VYKFRTMVNEPNPIVPDASRITRVGKILRRFSLDELPQLINVANGTMSIVGPRPTLQY
                     QVDRYSDFQRRRLEVRPGLTGLAQVNGRNALNWDRRIELDVEYVERQSLLLDLQIIAR
                     TIKTVLSGEGIDGHDATDPLVATVEATQPEPADSGDPPVEP"
     gene            817..1935
                     /locus_tag="PLP95_12450"
     CDS             817..1935
                     /locus_tag="PLP95_12450"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HPB46659.1"
                     /translation="MTEPGRRPRLRVGLVVSTPQRRGAEVFAVGLAERLRERDWCVEV
                     ASIDRSDAANVLEEITPLGDGRRSVAMWRNLVRLARRCDVLVASGGTTLLPVAAIARI
                     TATPYVYRNIGDPSAWGNVHGANLRLGLPLRSAAGVIALYGGAGDDLQRRYRLDARRI
                     TVAPNAASSNEFSPPTETERRKARAVFGFGIDQPVVVYLGALSEEKNPLLVIKAVLAE
                     PTLRLLVVGDGPLREKCDALAASAPDRVRVLGTRNDVPAVLAAADALVISSHTEGIAG
                     AAIEAGLSGVPVVTTDVGGMAELVLDGVTGLVLDRPDAATLGRALVKAANHRDPWGAA
                     AVEHCRAHYEFDAVTDLWVEALQAAADRRFPNPQAMGS"
BASE COUNT          340 a          707 c          571 g          318 t
ORIGIN      
        1 agagcctgac cctggatgtc aagcacgccg aagccgagaa gatattcgac gcccacttca
       61 ccgcatcgac cgacaacgga tacccggtca cgacctcgaa ccaccccgaa ttgacatcca
      121 ccggacctgc caaatactcg gtattgcccg gtacgctgtc gtcgtgggca ccaatacatc
      181 cggcagcggg cgggccagcc gacttcttga catcgtggtg ctgcttatcg ctgcgccggt
      241 gatcgttcca gtcggggtcc tgtgcgcgct cgccattcgg ctcgattcct ccgggcctgc
      301 gctctttcgc caggaacgag ttgggaagaa cggatcgccg ttcaccgtct acaagttccg
      361 caccatggtg aacgaaccca acccgatcgt tcccgacgcg tcacgcatca cccgcgtcgg
      421 caagatcctg cgacggttct ccctcgatga actcccccaa ctcatcaacg tcgccaacgg
      481 aaccatgtcg atcgttggcc cacgcccaac cttgcagtac caggtcgacc gctacagcga
      541 cttccaacgc cgccgtcttg aggtacgtcc gggcttgacc ggcctcgcac aggtcaacgg
      601 ccgcaatgcg ctgaactggg accgtcgcat cgaactcgac gtcgagtacg tcgaacgcca
      661 gtcgctgctc ctcgacctgc agatcatcgc ccgcaccatc aagacggtgc tcagcggtga
      721 aggcatcgac ggacacgatg ccacagaccc tctcgtggcg acggtcgagg cgacccaacc
      781 ggagccggcg gattccggtg atccgcctgt ggagccatga ccgagcccgg tcgacgccca
      841 cgattgcggg tcgggctcgt cgtttccaca cctcagcgcc gcggtgccga ggtgttcgcc
      901 gtcggcctgg ccgaacggct ccgcgaacga gactggtgcg tcgaggtcgc ctcgatcgac
      961 cggtcggatg cagcgaatgt cctggaggaa atcaccccgc tcggcgacgg ccggcgttcg
     1021 gttgccatgt ggcgaaacct cgtgcgcctc gcacggcgtt gcgacgtcct cgtcgccagc
     1081 ggtggcacca ccctgttgcc cgtcgcagcg atcgcccgca tcaccgccac gccctatgtg
     1141 taccgcaaca tcggcgaccc ctccgcctgg ggcaacgtcc acggcgcgaa ccttcgcctc
     1201 gggctcccgc tgcgaagcgc cgctggcgtc atcgcactgt atggcggtgc cggcgacgac
     1261 ctccagcgtc gataccgcct cgacgcccga cgcatcaccg tcgcccccaa cgctgcttcg
     1321 agcaacgagt tctccccgcc gaccgaaacc gaacgtcgca aggcacgagc ggtattcggt
     1381 ttcggtatcg accaaccggt cgtcgtctac ctcggcgcat tgtcggagga gaagaacccg
     1441 ctcctggtga tcaaggccgt cttggccgaa ccgacgctgc gcctcctcgt ggtcggcgac
     1501 ggaccgctac gcgagaagtg cgatgcactc gcagcatccg ctcccgaccg cgttcgcgtt
     1561 cttggaaccc gcaacgacgt gcccgctgtg ctcgcggccg ccgacgcact cgtgatctcg
     1621 agccacaccg agggcattgc tggcgccgcc atcgaagcgg gactcagcgg ggtccccgtc
     1681 gttacgaccg acgtgggtgg aatggccgaa ctcgtcctcg acggcgtgac agggctggtc
     1741 ctcgaccggc ccgacgccgc gaccctcgga cgcgcactcg tcaaggccgc caaccatcga
     1801 gacccttggg gcgcggccgc tgtcgagcac tgtcgagcgc actacgaatt cgacgccgtc
     1861 accgacctgt gggtcgaagc ccttcaggcc gcggccgacc gacgcttccc aaacccacaa
     1921 gcgatgggct cgtaac
//
LOCUS       DAOOMA010000556         1930 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_75160_length_1930_cov_4.600533, whole genome shotgun sequence.
ACCESSION   DAOOMA010000556 DAOOMA010000000
VERSION     DAOOMA010000556.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 1930)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1930)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1930
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_75160_length_1930_cov_4.600533"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(27..>1930)
                     /locus_tag="PLP95_12455"
     CDS             complement(27..>1930)
                     /locus_tag="PLP95_12455"
                     /inference="COORDINATES: protein motif:HMM:NF023418.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transglutaminaseTgpA domain-containing protein"
                     /protein_id="HPB46660.1"
                     /translation="RVAAARWGAAMSGEQVALDRRAASPRRTLAIGALSALSVGLAVS
                     LLPARVGGVAMAFGSGATVALLGVFGLRSVPLRRVVRGLCLLGVVIVVGWADLASSQV
                     DTTMVGLAWMAATAAALVAAQTTWGAAPGRPPQWSAPAVATATVGLVVAMATSAIVFS
                     PTVTGAFGDGVRSGASANPWDRQSSNPMAATSQLDMTRRPRLSDDLIMTVRSDAPGFW
                     RAQVFDVWDGRTWTISDAQLAPIGADGSVNSTDRDDLATATLHQPTEVVEQFRIEARF
                     SSSMPLSPVPLRVNADENINMVSLPDGSIVVPAGLGKGATYEVTSARQLDLTAEVLRS
                     TNEVEANARIADRYAQQPVATERVRRLAVELAAGADTTFDKVQAIEAWLDRNVTYSID
                     APLAPTDTDVVDDFLFESRVGWCEQIASSMTVLLREQGVPARLATGFVPDSRDAVSGR
                     YRVLENGAHAWTEVWFEGVGWVPFDPTAGIEVSGRASLGESSTGAVRTAAMIVGLVVV
                     LWVVLWPPVMGLVRRRGRGVRLFEAALSALDRRRRTRRLARSGEVADRVVIRLEEVAR
                     RHGLERSRDESGARFARRVGATLDVEGLDEVAAALDLRLYGPDGERDKAVPQLESALT
                     HLFSDNQWS"
BASE COUNT          339 a          698 c          622 g          271 t
ORIGIN      
        1 tgttctccga taaccagtgg tcgtgatcac gaccactggt tatcggagaa caggtgggtg
       61 agcgcggatt cgagctgagg gaccgccttg tcacgttcgc cgtcggggcc atacagccgc
      121 aggtcgagcg cggcggcgac ctcgtcgaga ccttcgacgt cgagggtcgc gcccacacgt
      181 cgggcgaacc tcgcaccgct ctcatcgcgg ctgcgctcga ggccgtgacg tcgtgccacc
      241 tcctcgaggc ggatgaccac acggtctgcg acctcgccgc tgcgcgccaa ccggcgcgtt
      301 cggcgacgtc gatcgagagc ggacaatgcc gcctcgaaca gccgcacacc gcggccgcgg
      361 cgacgaacga gtcccatcac cggtggccac agcaccaccc acaacaccac gacgagaccg
      421 acgatcatcg cagccgtgcg aaccgctccg gttgacgact caccgagact ggcccgaccc
      481 gacacttcga tgccggcggt cgggtcgaac ggcacccacc ccacaccctc gaaccacacc
      541 tcggtccaag cgtgcgcacc gttctccaaa acccggtacc gcccgctgac cgcatcgcga
      601 ctatcgggca cgaatccggt cgcgaggcgg gcagggaccc cctgttcgcg caagagcacc
      661 gtcatcgacg aagcgatctg ttcgcaccag ccgacgcggg actcgaacaa gaagtcgtcg
      721 acgacatcgg tgtcggtcgg ggcgagcggg gcgtcgatcg aataggtgac gttgcggtcg
      781 agccacgcct cgatcgcttg gaccttgtcg aaggtggtgt ctgcacccgc cgccagttcg
      841 accgcgagcc ggcgcacacg ctcggtcgcg acgggctgct gcgcgtaccg atcggcgatc
      901 cgtgcattcg cctccacctc gttcgtcgag cgaagcacct ccgcagtcag gtcgagctgt
      961 cgggcgctcg tcacctcgta ggtggcccct ttgcccaacc ccgcgggaac gacgatcgat
     1021 ccgtccggca gcgacaccat gttgatgttc tcatcggcgt tgacccgaag cggaaccggg
     1081 ctgagcggca tggaactgga gaaccgcgcc tcgatccgga actgctcgac gacctcggtc
     1141 ggctggtgga gcgtggcggt ggccaggtca tcgcggtcgg tcgagttgac ggaaccgtcg
     1201 gcgccgatcg gggccagctg agcatccgat atcgtccagg ttcggccgtc ccagacatcg
     1261 aacacctgcg ctcgccagaa ccccggggca tcgcttcgca ccgtcatgat caggtcgtcg
     1321 ctgagacgtg gtcgccgggt catgtcgagt tgactcgtcg cagccatcgg attcgacgat
     1381 tgccggtccc acggattcgc gctggccccc gaacgcacac catcgccgaa cgcgcccgtc
     1441 acggtcggcg agaacacgat ggccgaggtg gccatggcga ccacgaggcc gaccgtcgct
     1501 gtggcaacgg cgggcgccga ccattgcggt ggccggccgg gtgcagcccc ccaggtcgtt
     1561 tgcgcggcca ccagcgctgc ggccgtggcg gccatccacg ccagcccgac catggtggtg
     1621 tcgacctggg atgaggcgag gtctgcccag ccgaccacga tgacgacgcc caacagacac
     1681 agcccacgaa cgacccgacg aagcggcacc gaccgcaggc cgaacacccc caacagcgca
     1741 acggtcgctc cgctgccgaa cgccattgcc actcccccga cgcgcgccgg caacaagctg
     1801 acagcgagcc cgaccgacag agcgctcagg gcaccgatcg cgagggtgcg tcggggcgac
     1861 gccgcccgtc gatcgagcgc gacctgttcg ccactcatcg ctgcgcccca tcgggcggcg
     1921 gcgacgcgat
//
LOCUS       DAOOMA010000557         1929 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_75258_length_1929_cov_2.025080, whole genome shotgun sequence.
ACCESSION   DAOOMA010000557 DAOOMA010000000
VERSION     DAOOMA010000557.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 1929)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1929)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1929
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_75258_length_1929_cov_2.025080"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            275..1273
                     /locus_tag="PLP95_12460"
     CDS             275..1273
                     /locus_tag="PLP95_12460"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HPB46661.1"
                     /translation="MTPSQPTSSSASRVRIGLVGAGMMGTLHARVISESLRCELTWIC
                     DRNETSGRNAAARFTTRWVPEMEFNSVDAVIIASSTETHPALALAAIDAGKALLVEKP
                     LADTLADTMQIVDSAAAGDIPLMCGFLERFNPVIRTAMEFVEAPVHAAAARHSPYVTR
                     IRTGVASDLLIHDLDALIRLFGTRPTLQRGQLGEVHPDSDPGSEDIAEAILDFGAGRL
                     ATASASRVAQRKVRTLVITELDRQIELDLLRQDITIYRHVEHAASDDRTGLGYRQQTV
                     IDIPVLRFQREPLAAQLDEFLDVVAGDHDLTAVRESLLEPHLALGALRASITSAES"
     gene            1275..>1929
                     /locus_tag="PLP95_12465"
     CDS             1275..>1929
                     /locus_tag="PLP95_12465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB46662.1"
                     /translation="MAQRRAAQLGGLIRSGQSATAIGLAFNGLAAYVFLAAAGRSLGP
                     AEFAPLSVLWTMLFLVATGLFQPFEQELARAVSANRSLGLGYSEVVRRNAVVAAALVA
                     CVILIGAIAAGPIAEGFFRGERSFVIALVVGIAGIGFMFFVRGLLAGSGRFYGFAVLF
                     VADGLTKSLPAVIFWLAGGRNPSVFVAVLVAGGFVGGAAPLTRGGVRATDPGPIPQWG
                     "
BASE COUNT          313 a          693 c          573 g          350 t
ORIGIN      
        1 gcacgttggc gcggccaagg catcagccgc cacaagctga tcctccggct cccgtgactt
       61 ccgagctgcc acaagcgatg ccgacgcttc gacccgcccg agctctgtcg gccgaacagc
      121 acatcccgta cgtcacgatc gtcggtttcg acatcgagct tcagccgacg aggccatagc
      181 ccagcccgcc gacgacacga cgaaggcatg gccgcgcaca accgacgcgg tagggtgcat
      241 cggcttccgc gcaacgaacg aactggagcc gagcgtgacc ccttcccagc caacttccag
      301 ctccgcttcc cgagtccgaa tcggcttggt cggagcgggc atgatgggaa cgcttcacgc
      361 acgggtcatt tccgaatcgc tccgttgcga gctgacgtgg atctgcgatc gcaacgagac
      421 gagcgggcga aacgctgcag cccgcttcac cactcgttgg gtcccggaaa tggaattcaa
      481 ctccgtcgac gccgtcatca tcgcctcatc gaccgagacc catccggcgt tggccctcgc
      541 tgcaatcgac gctggcaaag cgctcctcgt cgaaaagccg ttggcggaca cccttgcgga
      601 cacgatgcag atcgtcgatt ccgccgccgc tggcgatata ccgctcatgt gcgggttcct
      661 cgaacgtttc aatcccgtca tccgcacggc catggaattc gtcgaggcac cggtccacgc
      721 cgccgcggct cgccactccc cctatgtcac ccgaattcgc accggtgtgg cctcggacct
      781 gctcattcac gacctcgatg cactcatccg tttgttcggc acgcgcccca cgttgcagcg
      841 tggccagctg ggagaggtac accccgatag cgatcccggt tccgaggaca tcgccgaagc
      901 catcctcgat ttcggtgccg gtcgactggc gactgcgtca gcgagccgcg tcgctcaacg
      961 caaagtacgg acgctggtga tcaccgaact cgaccgccag atcgaactcg acctgctccg
     1021 ccaggacatc accatctacc gccacgtcga acacgccgca tccgatgacc gcaccggact
     1081 cggataccgc caacagaccg tcatcgacat ccccgtcctc aggttccaac gcgaaccgct
     1141 cgccgcgcag ctcgacgaat tcctcgacgt ggtcgccggc gaccacgacc tcacggccgt
     1201 gcgggagagc cttcttgagc cgcaccttgc actcggggcc ctgcgcgcaa gtatcacctc
     1261 tgcggagagc tgaggtggcc cagcgcaggg ccgcccaact gggcggtctg attcgctcgg
     1321 gacagtcagc gaccgctatc gggctggcgt tcaacggtct ggcggcgtac gtattcctcg
     1381 ccgcggccgg acggtccctc ggccctgcgg agttcgcgcc gctgtccgtg ctttggacga
     1441 tgttgttcct cgtggcaacg gggttgtttc agccgttcga acaggaactc gcccgcgccg
     1501 tctccgccaa ccggtcactc ggcctcggct acagcgaggt cgtacgacgc aacgccgtcg
     1561 tcgccgcagc gctcgtcgcc tgcgtgatcc tcatcggagc catcgccgct ggaccgatcg
     1621 ctgaaggctt ctttcgaggg gagcgctcct ttgtcattgc gctcgtcgtc ggcatcgccg
     1681 gcatcgggtt catgttcttt gtgcgcggac tactcgcagg ctccgggcgc ttctacggat
     1741 tcgccgtcct cttcgtcgcc gacggcttga cgaagtcgct tccggccgtg atcttctggc
     1801 tggcaggtgg ccgcaaccct tcggtcttcg tcgccgttct cgtcgccggc ggattcgtgg
     1861 gaggggccgc cccactgacg cgcggcggtg tgcgggcgac cgaccctgga cccatcccgc
     1921 agtggggcg
//
LOCUS       DAOOMA010000558         1928 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_75298_length_1928_cov_3.633209, whole genome shotgun sequence.
ACCESSION   DAOOMA010000558 DAOOMA010000000
VERSION     DAOOMA010000558.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 1928)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1928)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1928
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_75298_length_1928_cov_3.633209"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..406
                     /locus_tag="PLP95_12470"
     CDS             <1..406
                     /locus_tag="PLP95_12470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB46663.1"
                     /translation="LKFEFLPYFRVQLAQLGSGAAAATATAAAAPVSAPFGAGAQAAA
                     PKPAEVAPIAAPPAAPVFGAEAAAAETTPEPPAGDVRVGAVTLGSASAPTSGGDAPTN
                     LDSPVGASSTAPNGSTFGAPEEERGRRGLFGR"
     gene            complement(555..968)
                     /locus_tag="PLP95_12475"
     CDS             complement(555..968)
                     /locus_tag="PLP95_12475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB46664.1"
                     /translation="MFASLIIGLAIIVVGAVLSGWMLFGQNGSVDTSAHHVHSTLTAP
                     PTNAVATAGGSQVSSNTADTAPVATGRGRFERRPGGVRDTMGMARRDGSRNVKVGWLV
                     RLRSAALLVLITVGIGTVIGALFGMVALAASFVIG"
     gene            967..>1928
                     /locus_tag="PLP95_12480"
     CDS             967..>1928
                     /locus_tag="PLP95_12480"
                     /inference="COORDINATES: protein motif:HMM:NF017200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HPB46665.1"
                     /translation="MTDPIVSTTRSRRVWPWVAAVLLVVAVVVAFVVPVPYYLEGPGS
                     TRATQQFITISGHESFESEGQVRLVTVSQRHATLAALALGWLRPNIDIVSERDVIGTV
                     DRKTERLINQAQMDRSKLVALVAAFDVLGLPITITGHGALVQGFAEDEVPARSVLQVG
                     DVITAVGERSIATTTELRSALDGAAPGDVVTVTVSRLESQDPLQALIPSAQRHELAVE
                     VELARNPEEDRAMLGVTVQTFNETVDTGFSVEFDSGGVLGPSAGLAWTLGLIDRLTPG
                     DLTGGRVVGVTGEIDAAGNVGAIGGVDKKVVGAIRDGATVFLYPS"
BASE COUNT          305 a          648 c          669 g          306 t
ORIGIN      
        1 gctcaagttc gagttcctcc cgtacttccg agtccagctc gcgcagctcg gctcgggtgc
       61 cgctgcggcc accgccacag cggccgcagc gccggtgtcg gcgcccttcg gtgccggcgc
      121 tcaggcggcc gcaccgaagc ccgccgaggt agccccgatt gccgcaccac ccgcagcgcc
      181 ggtgttcggc gccgaagcgg ccgcagcgga gacgaccccc gagccccccg caggtgatgt
      241 tcgcgtcggc gccgtcaccc tcggaagcgc ctcggccccc acctcgggcg gagatgcccc
      301 gaccaacctc gactcgccgg tcggtgccag cagcaccgct cccaacgggt cgacgttcgg
      361 cgctcccgaa gaagagcgcg gccgccgcgg attgttcggt cgctgaacct cccccaaggt
      421 cgtcaagggt tcgccgggtc ctgcagggtc cggcgaacct tcgtttttgg ggcgacgatc
      481 cgcgtgaggc ggcgccggcc cggcgaaggg gctcagccga aggggctcag ccgaaggggc
      541 tcagccgaag gggctcagcc gatgacgaaa ctggccgcga gcgcgaccat gccgaacagt
      601 gcgccgatca ccgtgccgat ccccacggtg atcaacacca acaacgcggc tgagcgcagc
      661 cggacgagcc agccgacctt gacgttgcgc gagccatctc ggcgagccat gcccatcgtg
      721 tcgcgaacgc caccaggcct gcgctcgaac cgaccacggc cagtggcgac gggtgcggta
      781 tcggccgtgt ttgaactcac ctgagaacct ccagcggttg cgaccgcgtt cgtgggtggt
      841 gccgttaggg tgctgtgcac gtgatgggct gaggtgtcga ccgagccgtt ctggccgaac
      901 aacatccacc ccgacaacac cgcacccacc acaatgatgg ccaacccgat gatgagtgac
      961 gcgaacatga cagaccccat cgtatcgacg acgcggtcga ggagggtgtg gccatgggtt
     1021 gcggcagtgt tgctggtggt ggcggtcgtg gtcgcgttcg tcgtgccggt gccgtactac
     1081 ctcgaggggc cgggctccac ccgtgccact cagcagttca tcaccatcag cggccacgag
     1141 tcgttcgagt cggagggtca ggtgcggctc gtcaccgtgt cgcaacgtca tgcgaccctc
     1201 gccgccctcg cgctggggtg gcttcgaccc aacatcgaca tcgtcagtga acgcgacgtg
     1261 atcggtacgg tcgatcgcaa gaccgaacgc ctcatcaatc aggcacagat ggaccgttcg
     1321 aagctggtgg ccctcgtggc ggctttcgac gtgttggggc tccccatcac catcaccgga
     1381 catggcgcgc tcgtgcaggg attcgccgag gacgaggtgc cggcgcggtc ggtgctgcag
     1441 gtcggcgacg tgatcaccgc cgtcggtgag cgctccatcg cgacgaccac cgagttgcgc
     1501 agtgccctgg atggggccgc gcccggcgat gtcgtcacgg tgaccgtttc gcggctcgag
     1561 tcgcaggacc cgctgcaggc gttgattcct tcggcgcagc gacacgaact cgcggtcgag
     1621 gtggagctcg ccaggaaccc cgaggaggac cgggcgatgc tcggcgtgac ggtgcagacg
     1681 ttcaacgaga ccgtcgacac gggattctcg gtggaattcg actcgggcgg ggtgctcggg
     1741 ccgtctgccg ggttggcgtg gacactcggt ttgatcgacc gtctcacccc tggggatctg
     1801 accggcgggc gggtcgtcgg cgtcacggga gagatcgacg ctgccggcaa tgtcggtgcg
     1861 atcggcgggg tcgacaagaa ggtggtcgga gcgatccgcg atggggccac ggtgtttctc
     1921 tatccctc
//
LOCUS       DAOOMA010000559         1914 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_76138_length_1914_cov_3.291017, whole genome shotgun sequence.
ACCESSION   DAOOMA010000559 DAOOMA010000000
VERSION     DAOOMA010000559.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 1914)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1914)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1914
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_76138_length_1914_cov_3.291017"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..520
                     /locus_tag="PLP95_12485"
     CDS             <1..520
                     /locus_tag="PLP95_12485"
                     /inference="COORDINATES: protein motif:HMM:NF014874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cyanophycin synthetase"
                     /protein_id="HPB46666.1"
                     /translation="ALAHVVAPPGRFETIAEGQPFHVVVDYAHTPDALENLLGAAREL
                     CDPADGAGGRLRVVFGCGGDRDASKRPRMGEVAQRLADSVTVTSDNPRSEDPRAIIDA
                     INAGRRRTDDTIVDRREAIEAVLASAGPGDVVVIAGKGHETTQTIGNTVLPFDDREVA
                     RAWLRSRDWGRS"
     gene            487..1572
                     /gene="mraY"
                     /locus_tag="PLP95_12490"
     CDS             487..1572
                     /gene="mraY"
                     /locus_tag="PLP95_12490"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012224655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HPB46667.1"
                     /translation="MASLTRLGSLMIRIFLAAGLASVLSLFGTKYLIDVLTRLKFGQP
                     IHEDVPEGHTVKAGTPTMGGIAIVISAAISYAVSGRYRGVHTERGLYVMMAIVGAGFV
                     GFLDDFIKIRNERNLGLGKGAKMFGLLSVAVGFSVLMVTQTKQSTTLSFTRFNNFESW
                     ADLGKVGWAIWAVLLIVGASNAVNLTDGLDGLAAGSAIFGFGAYVLIGFWTFRNPIVY
                     NLIGGDELTVGLDLAVVAAAMMGACGGFLWWNANPAKIFMGDTGSLAIGVAFACLALT
                     TNTALLLPVIGALFVAETVSVILQVISFRKFGRRIFRMAPIHHHFELAGWPETTVIIR
                     FWMLSAACTAVGVGLFYYDFFKVTNTL"
     gene            1572..>1914
                     /locus_tag="PLP95_12495"
     CDS             1572..>1914
                     /locus_tag="PLP95_12495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB46668.1"
                     /translation="MTVDRLAERSLLLWGWGVTNAAVAAALTARGRRVVVADDRPTPD
                     KLAEAAALGLGIETPASRDEIAWLVADVDAFVPAPGLPESHPVFAAAQGAGVALVSEF
                     DLAQAFDDRPFV"
BASE COUNT          287 a          619 c          650 g          358 t
ORIGIN      
        1 ggccttggcg cacgtcgtcg caccacccgg acgctttgaa acgatcgccg aggggcagcc
       61 gtttcatgtc gtcgtcgact acgcgcacac cccggacgcg ctggagaatc tcctcggtgc
      121 ggctcgcgaa ctgtgcgatc cggccgacgg ggccggcggc cgcctgcgcg tcgtgttcgg
      181 gtgcggcggc gatcgcgatg cgtcgaagcg gccgcgaatg ggagaggttg cccagcgtct
      241 ggcggattcg gtgacggtca cctcggacaa cccccgttcg gaggaccccc gagccatcat
      301 cgacgccatc aatgccggtc ggagacggac cgacgacacg atcgtcgatc gtcgcgaggc
      361 catcgaggcg gtccttgcct cggcagggcc cggcgacgtg gtcgtcatcg ccggcaaggg
      421 tcacgaaacg acccagacca tcggcaatac cgtgttgccc ttcgacgacc gtgaggttgc
      481 ccgtgcatgg cttcgctcac gcgattgggg tcgctcatga ttcgtatctt cctggccgcc
      541 ggcctggcct cggtgctttc gctgttcgga acgaagtacc tcatcgacgt gttgacccgg
      601 ctgaagttcg gtcagccgat ccacgaggac gttccggagg gccacacggt caaagcgggc
      661 accccgacca tgggcggaat cgccatcgtg atctcggcgg ccatttccta tgccgtgagc
      721 ggccgctacc ggggggtcca caccgaacgt gggctgtacg tgatgatggc catcgtcggc
      781 gccgggttcg tcggcttcct cgacgacttc atcaagattc gcaacgaacg caacctcggc
      841 ctcggcaagg gggcgaagat gttcggcctg ttgtcggtgg cggtcgggtt ctcggtgttg
      901 atggtcaccc agaccaaaca gagcaccacg ctgagtttca cccgattcaa caacttcgaa
      961 tcgtgggccg atctcggcaa ggtcggatgg gcgatctggg cggtgctgtt gatcgtgggt
     1021 gcctccaatg cggtgaacct caccgacggc ctcgacggcc tcgccgccgg cagcgccatc
     1081 ttcgggttcg gcgcgtacgt gctcatcggg ttctggacct tccgaaaccc gatcgtctac
     1141 aacctgatcg gcggcgacga actgaccgtc ggcctcgacc tggcggtggt ggccgccgcg
     1201 atgatgggcg cctgcggggg gtttctctgg tggaacgcca acccggcgaa gatcttcatg
     1261 ggcgacaccg gctcgctcgc gatcggcgtc gcgttcgcat gtctcgcgct cacgacgaac
     1321 accgcgctgc tgctgccggt gatcggagcg ttgttcgtcg ccgaaacggt gtcggtgatc
     1381 ctgcaggtca tcagcttccg taagttcggc cgccggatct tccgcatggc gccgatccat
     1441 catcacttcg agctcgccgg ctggccggag accacggtga tcatccggtt ctggatgctg
     1501 tcggccgcgt gcaccgccgt cggggtggga ctgttctatt acgacttctt caaggtgacc
     1561 aacacgctgt gatgaccgtc gatcgactcg ccgagcgctc gctactgctc tggggctggg
     1621 gcgtcaccaa cgccgcggtc gcagcggcgt tgacggcgcg cggacgccgg gtcgtggtcg
     1681 ccgacgatcg cccgaccccc gacaagctcg ctgaagctgc ggcgctgggg ttgggaatcg
     1741 agacgccggc gagtcgcgac gagatcgcat ggctcgtggc ggacgtcgac gcgttcgtgc
     1801 cggctcccgg gctgccggag tcgcatccgg tgttcgccgc ggcacaaggt gccggggtgg
     1861 cgctcgtgag cgagttcgat ctggcccagg ccttcgacga tcgcccgttc gtgt
//
LOCUS       DAOOMA010000560         1914 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_76162_length_1914_cov_2.280258, whole genome shotgun sequence.
ACCESSION   DAOOMA010000560 DAOOMA010000000
VERSION     DAOOMA010000560.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 1914)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1914)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1914
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_76162_length_1914_cov_2.280258"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..616)
                     /locus_tag="PLP95_12500"
     CDS             complement(<1..616)
                     /locus_tag="PLP95_12500"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012229379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HPB46669.1"
                     /translation="MKHLLSIDDLTRGDIEGLLDLTDRFVEVAHRAVPKVPALRGRTV
                     VSLFFEDSTRTRLSFETAAKRLSADVMSFSVSTSSVKKGESLRDTVETITAFGVDAII
                     IRHRSSGAPLQITRWTDAQVLNDGDGWHQHPTQALLDSYTIRQRFGSLDGLRIGIIGD
                     IQHSRVARSDVDAFTKLGAEVVLVAPPTLLPPSLEGWPVTVTHDL"
     gene            complement(613..1182)
                     /gene="pyrR"
                     /locus_tag="PLP95_12505"
     CDS             complement(613..1182)
                     /gene="pyrR"
                     /locus_tag="PLP95_12505"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012229378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HPB46670.1"
                     /translation="MADRERRGAPPYGAEFQARTVVMDSDDIGRAIRRMAHEIVERNH
                     GLDAVALVGLQTGGVPLAEAIAADLSEIESVTVEVGRLDVAFYRDDIGLRPVLTEEVT
                     DIAFDVDNRVIVLVDDVLFTGRTIRAALDALSSYGRPRSVQLAVMVDRGHRELPIRPD
                     YVGKNLPTRRDEMVNVSALGVELGEMVRK"
     gene            complement(1266..1700)
                     /gene="nusB"
                     /locus_tag="PLP95_12510"
     CDS             complement(1266..1700)
                     /gene="nusB"
                     /locus_tag="PLP95_12510"
                     /inference="COORDINATES: protein motif:HMM:TIGR01951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HPB46671.1"
                     /translation="MANSYSPGFVPSEPGRHEARERAVALGYEAEQRDLDAAGILDDQ
                     ILDPDPYTVALVAGVGEHLEEIDTTIERFARGWTLERMAAMDRAVLRVAIFELAHRED
                     IPTPVVIDEAVELAKRYGTDDSSRFVNGVLDSAANALRSTGS"
     gene            complement(1703..>1914)
                     /locus_tag="PLP95_12515"
     CDS             complement(1703..>1914)
                     /locus_tag="PLP95_12515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012229376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HPB46672.1"
                     /translation="EIIGVELPASVDLPISHTEPGLQGDRSSAGRKPATLVTGLVVQV
                     PLFIGPDDVIRVDTRTGEYITRANG"
BASE COUNT          310 a          628 c          650 g          326 t
ORIGIN      
        1 cgagatcgtg ggtcaccgtc accggccaac cttccagcga gggcggcaac agcgtcggtg
       61 gggccaccag caccacctcg gcgcccagct tggtgaacgc gtcgacatcg gagcgggcga
      121 cacgcgaatg ctggatgtcg ccgatgatgc cgatgcgaag accgtcgagc gaaccgaaac
      181 gctgccggat cgtgtagctg tcgagcagcg cctgggtcgg gtgctggtgc catccgtcgc
      241 cgtcgttgag cacctgcgcg tcggtccatc gggtgatctg cagcggcgcg cccgagctgc
      301 ggtggcggat gatgatggca tcgacgccaa acgcggtgat cgtctccacc gtgtcgcgca
      361 gcgactcccc cttcttcacc gacgaggtcg acaccgagaa actcatgacg tcggccgaga
      421 ggcgtttggc cgccgtctcg aagctcaacc gggttcgggt ggagtcctcg aagaacagcg
      481 acacgaccgt gcgcccgcga agggccggga ccttggggac cgcgcggtgg gccacttcga
      541 cgaaccggtc ggtgaggtcg agcagcccct cgatgtcgcc gcgggtcagg tcgtcgatac
      601 tcagcaggtg cttcatttgc gcaccatttc gccgagttcg acccccaagg ccgacacgtt
      661 gaccatctcg tcacgcctcg tcggcaggtt cttgcccaca tagtcggggc gaatcggcag
      721 ttcgcggtgg ccgcggtcga ccatcacggc gagttgcacc gacctcgggc ggccatagga
      781 actcagtgcg tcgagcgcgg cgcgaatggt gcggccggtg aacaacacgt cgtcgacgag
      841 cacgatcacg cggttgtcga cgtcgaaggc gatgtcggtg acctcctcgg tcaacaccgg
      901 ccgcagcccg atgtcgtctc gatagaacgc gacgtcgagc cggccgacct cgacggtgac
      961 cgattcgatc tcgctgaggt cggcggcgat cgcctccgcg agcggcactc ccccggtctg
     1021 gaggccgacg agggccacgg cgtcgaggcc gtggttccgt tcgacgatct catgtgccat
     1081 gcgacgaatc gcacgaccga tgtcgtcgga atccatcaca acggttctcg cctggaactc
     1141 agcgccgtat ggcggggctc cgcgacgctc gcggtcggcc attcgcacac tcctcgcccg
     1201 aaggcactcc gtggaggcct ctccggaccc ccgttaacgg tgtcgacgcc gaggttacgg
     1261 gacgttcagg agcccgtcga acgcaacgcg ttcgctgcgc tgtcgagcac gccgttgacg
     1321 aatcggctcg aatcgtcggt gccgtagcgc ttcgccagtt cgaccgcctc gtcgatcacc
     1381 accggggtcg ggatgtcttc acggtgggcg agttcgaaaa tcgcgacccg cagcaccgca
     1441 cggtccatgg ccgccatgcg ctcgagggtc caacctcggg cgaagcgttc gatcgtggtg
     1501 tcgatctctt cgaggtgttc gccgaccccc gccaccagtg cgacggtgta ggggtcgggg
     1561 tcgaggatct gatcgtcgag gatgccggcc gcatcgaggt cgcgttgttc ggcttcgtag
     1621 ccgagcgcca ccgctcgctc gcgtgcctcg tggcgacccg gctctgatgg gacgaatccc
     1681 ggtgaatacg aattggccac ggtcagccgt tggctcgggt gatgtactcg cccgttcggg
     1741 tgtcgacacg gatgacgtcg tcggggccga tgaagagcgg aacctgcacc acgagtccgg
     1801 tcacgagcgt cgccggtttg cggcccgccg agctgcggtc gccctgcagg cccggctcgg
     1861 tgtgcgagat gggcaggtcg accgaggccg gcaactcgac accaatgatc tcgt
//
LOCUS       DAOOMA010000561         1913 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_76192_length_1913_cov_4.038751, whole genome shotgun sequence.
ACCESSION   DAOOMA010000561 DAOOMA010000000
VERSION     DAOOMA010000561.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 1913)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1913)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1913
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_76192_length_1913_cov_4.038751"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..895
                     /locus_tag="PLP95_12520"
     CDS             <1..895
                     /locus_tag="PLP95_12520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alpha-isopropylmalate synthase regulatory
                     domain-containing protein"
                     /protein_id="HPB46673.1"
                     /translation="INGYGERTGNANLITVIPNLTLKMGVTTLPEGNISRLTAVSRHV
                     AELVNLPPHPADPYVGSSAFAHKGGLHSSALAKVGSASYEHIDPAVVGNRTRVLVSDL
                     GGRAGMAMKAEEFGVDLDARQAAEVSDRIKVLEAEGYVFEAADASLELLMRRAGGWDN
                     PFFRVEAYRVSSYHREGDVAAEGHDVEVSTEATVKVWVGDERIAAVGEGNGPVNALDA
                     ALRNALEARFPAISDIHLVDFKVRILDTSASTDAVTRVLIDSTNGDDTWTTIGVSTNV
                     IEASWDALIDAITFGLLHRTS"
     gene            929..1426
                     /locus_tag="PLP95_12525"
     CDS             929..1426
                     /locus_tag="PLP95_12525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB46674.1"
                     /translation="MSAPEFVPTSPTARKSYQSPKWRPGSWHADRPGELDGRQPEGDR
                     LGAPGPDPGYVDRLVDTVESSLKLGHHEHLADVREAIAVVALKRASLFGRAPVIHDVT
                     AAVTALGFDQAPPSGDAGERRAALLEECHHPHFYVRLRSIADLVEAEVLAQPAAKITS
                     AVANW"
     gene            complement(1475..>1913)
                     /locus_tag="PLP95_12530"
     CDS             complement(1475..>1913)
                     /locus_tag="PLP95_12530"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HPB46675.1"
                     /translation="LPVRPNGTQGWVHTSQVRTSTIHAHVAINLAERRLVATVDGTVI
                     ADTPVVIGAADTPTPTGRLFLTHYDEKSEGSAYGPWVAALSGFSQALDRFSGGVPVIA
                     IHGTNNPAAVGQARSNGCIRIPNDVIRVLRDSLPLGTPVDIWP"
BASE COUNT          301 a          628 c          673 g          311 t
ORIGIN      
        1 gatcaatggc tacggcgaac gaaccggcaa cgccaacctg atcaccgtca tcccgaacct
       61 cacgctcaag atgggggtga cgacccttcc cgagggcaac atcagccgac tcaccgcggt
      121 cagccgccat gtggccgaac tcgtcaacct ccccccgcat cccgccgatc cctacgtggg
      181 ttcctcggcg tttgcgcaca agggcggcct gcactcctcg gcgttggcga aggtcggaag
      241 cgcctcctat gagcacatcg acccggcggt ggttggcaac cggacgcggg tgttggtgtc
      301 cgacctcggc gggcgggcgg ggatggcgat gaaggccgag gagttcggcg tcgacctcga
      361 tgcgcgtcag gccgccgagg tgtccgatcg gatcaaggtc ctcgaggccg aggggtatgt
      421 gttcgaggcc gccgacgcct cgctcgagct gttgatgcgg cgggccgggg gatgggacaa
      481 cccgttcttt cgggtcgagg cctatcgggt gtcgtcgtat caccgcgagg gtgatgtcgc
      541 ggccgagggt cacgacgtcg aggtgtcgac cgaggcaacg gtgaaggtgt gggtcggcga
      601 cgaacgcatc gctgcggtgg gggagggcaa cggcccggtc aacgccctcg acgcggcgtt
      661 gcgcaacgcg ttggaggcgc gattcccggc gatttccgac atccatctgg tcgacttcaa
      721 ggtccggatc ctcgatacct ccgcgagcac cgatgcggtc acccgggtgc tgatcgactc
      781 gacgaacggc gacgacacgt ggaccaccat cggggtgagc accaacgtca tcgaggcgtc
      841 gtgggacgcg ctcatcgacg ccatcacctt cggcctgttg caccggacgt cgtgaccgtg
      901 gcggcgcttc gataatcttg ggggcactat gtctgcaccc gagttcgttc ccacgtcgcc
      961 gaccgctcgc aagtcctacc agtcgccgaa gtggcgcccg ggttcgtggc acgccgatcg
     1021 accgggtgaa ctcgacggtc gacagccgga aggcgaccga ctcggcgctc cgggacccga
     1081 cccgggctat gtcgatcgtc tcgtcgacac cgtcgagtcg tcgctgaagc tcggccacca
     1141 cgagcacctc gcggatgtcc gcgaggccat cgccgtcgtc gcgctcaagc gggcctcgtt
     1201 gttcgggcgg gctccggtga ttcacgacgt gacagcggcc gtcaccgcgc tcgggttcga
     1261 tcaggcgcca ccctcgggcg acgcgggcga gcggcgggcc gcgttgttgg aggaatgcca
     1321 ccacccgcac ttctacgttc ggttgcgcag catcgccgac cttgtcgagg ctgaggtgct
     1381 ggcacagccc gccgcgaaga tcaccagcgc cgtcgccaac tggtaacacc gcgacgctgg
     1441 cggccggcgg ccggcggcgg acggcgccaa tggctcaggg ccagatgtcg accggcgtgc
     1501 cgagcggcag cgagtcgcgc aacacccgga tcacgtcgtt cgggatgcga atgcagccat
     1561 tcgatcgagc ctgtcccacc gcggccgggt tgttcgtccc gtggatggcg atgaccggca
     1621 cgcccccaga aaacctgtcg agcgcctggc tgaaccccga cagtgccgcc acccacggac
     1681 cgtaggccga gccctcggac ttctcgtcgt agtgggtgag gaagagtcgc ccagtgggcg
     1741 tcggggtgtc ggcggcgccg atgacgaccg gggtgtcggc gatgaccgtc ccgtcgacgg
     1801 ttgcgacgag tcgtcgttca gcgaggttga tggcgacgtg agcgtggatg gtcgaggtgc
     1861 ggacctggct ggtgtggacc catccctggg ttccgttcgg gcgcaccggc aac
//
LOCUS       DAOOMA010000562         1899 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_77005_length_1899_cov_2.894794, whole genome shotgun sequence.
ACCESSION   DAOOMA010000562 DAOOMA010000000
VERSION     DAOOMA010000562.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 1899)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1899)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1899
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_77005_length_1899_cov_2.894794"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..964)
                     /locus_tag="PLP95_12535"
     CDS             complement(<1..964)
                     /locus_tag="PLP95_12535"
                     /inference="COORDINATES: protein motif:HMM:NF020084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FIST N-terminal domain-containing protein"
                     /protein_id="HPB46676.1"
                     /translation="MRFAAVLSEHPLATQAVGEVVGQVLESLDAAPDIAMVFVSRSHG
                     GAIEDIARVVRRLLNPRVLVGATAAGVLGGGQEIEGGAAISLWAARFGVDVEPVRLEA
                     IPTAEHLLVAGGAALQADEGTLLLLADPFSFAMDQVIDHLRVVAPGVTMVGGMASGAL
                     GPGGNTLVLDDERFTTGAVGVWLPDTVDVRAAVSQGCRPIGEPFVVTRAERNVLYEIG
                     GRSAVERINELVESSTDAEKRLMAAGLHIGRVIDEARLEHGRGDFLVRSVLGADRSVG
                     AVAIDDEIPVGATVQFQVRDAASAHEDLVSRLRELHEGGVAGALA"
     gene            complement(1092..1475)
                     /locus_tag="PLP95_12540"
     CDS             complement(1092..1475)
                     /locus_tag="PLP95_12540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB46677.1"
                     /translation="MQILSALFLDEINLRQVAGPSTRIDMSGIQFSAVPPGPYPVTVS
                     PHLVVIIRCAPGENTVAALEVTFLRDGEQIARNAQPVDIEQGKFGYRLVRAELSYDEP
                     GTVEAHVRLDGGPATVVPYSLLAAG"
     gene            1549..>1899
                     /locus_tag="PLP95_12545"
     CDS             1549..>1899
                     /locus_tag="PLP95_12545"
                     /inference="COORDINATES: protein motif:HMM:NF024938.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HPB46678.1"
                     /translation="MSSPASSPLTRPATHTLSEAESKAFLEPFGVSIAPERVVATAEA
                     AVAAAEEIGWPVVAKLCGDTIAHKTERGLVRLHLTGADATRQAAQSLLDAALPDDGEV
                     GVLVTAMISGTRELI"
BASE COUNT          311 a          676 c          636 g          276 t
ORIGIN      
        1 aggccagcgc accggcgacg ccgccctcgt ggagctcgcg aagcctggat acgaggtcct
       61 catgggcgga ggcggcatcg cgcacctgga actgcaccgt ggcgccgacc gggatctcgt
      121 cgtcgatcgc caccgcaccc accgaacggt ctgctccgag cacgctgcgg accaggaagt
      181 cgccgcggcc gtgttcgagc cgggcctcgt cgatcacccg gccgatgtgc aaccccgcgg
      241 ccatcagtcg cttttcggca tcggtgctcg attcgaccag ttcgttgatg cgttcgactg
      301 cgcttcggcc gccgatctcg tacaacacgt tgcgttcggc gcgggtgacg acgaatggct
      361 cgccgatcgg tcgacaaccc tgtgataccg cggcgcgaac atcgaccgtg tcgggcagcc
      421 ataccccgac cgcgccggtg gtgaaccgtt cgtcgtcgag gaccagggtg ttgcccccag
      481 ggccgagcgc ccccgaggcc atcccgccga ccatggtcac ccccggggcc acgactcgca
      541 ggtggtcgat cacctggtcc atggcgaagg agaacggatc ggcgagcagc aacaacgtcc
      601 cctcgtcggc ctgaagcgcg gcgccaccgg cgaccaacag atgctcagcg gtggggatcg
      661 cctcgaggcg gacgggttcg acatcgacgc cgaaccgggc ggcccacagc gatatggcag
      721 caccgccctc gatctcctgg ccgcccccga ggacgccggc ggcggtcgcc ccgacgagca
      781 cccgcgggtt gaggagcctt cggaccacgc gggcgatgtc ctcgatggcc ccgccatggg
      841 atcgactgac gaacaccatg gcgatgtcgg gggcggcgtc gagggattcg agcacctgac
      901 caacgacctc gccgaccgcc tgcgtggcga gcgggtgctc gctgagcacg gcggcgaacc
      961 tcatgtgacg cctcgcaggc ggtcaccggc ttcgggccgc cgagcatcgc ccccgcctgg
     1021 gttctgtagg tactttcggt ggccctggcc accgaaagta cctacagaac ggttgagcgg
     1081 ctggcgaggg ttcacccggc ggcgagcaag ctgtagggca cgaccgtcgc cgggccaccg
     1141 tcgaggcgca cgtgggcctc caccgtgccc ggttcgtcgt agctcaactc cgcccgcacg
     1201 aggcgatagc cgaacttgcc ctgttcgatg tcgacgggct gagcgttgcg ggcaatctgt
     1261 tccccgtcgc gcaggaacgt cacctcgagc gccgccaccg tgttttcgcc tggcgcgcag
     1321 cggatgatga ccaccagatg aggcgacacc gtcaccggat aggggcccgg gggcaccgcc
     1381 gagaactgga tgccgctcat atcgatgcga gtcgatggac cggccacctg gcgaaggttg
     1441 atctcgtcca ggaagagtgc ggagagaatc tgcatggctg cgacgctagt gccgacgcga
     1501 ggaccgaggg ccacggtcac aaccgcctcg acgcgtagga tggccgccat gtcctcacca
     1561 gcttcgtcgc ccctcacccg gcccgcgacc cacacgctgt ccgaagccga gtccaaggcg
     1621 ttcctcgagc cgttcggcgt gtcgatcgcc cccgaacgcg tcgtggccac cgccgaggcg
     1681 gcagtcgccg ccgccgagga gatcggttgg ccggttgtcg caaagctctg cggggacacc
     1741 atcgcccaca agaccgaacg tggcctggtg cgccttcatc tcaccggcgc cgacgcgacg
     1801 cgacaggccg cccagtcgct gctcgacgcc gcactgcccg acgacggaga ggtcggcgtg
     1861 ctcgtcactg cgatgatcag cggcacccgg gaactcatc
//
LOCUS       DAOOMA010000563         1894 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_77312_length_1894_cov_2.707993, whole genome shotgun sequence.
ACCESSION   DAOOMA010000563 DAOOMA010000000
VERSION     DAOOMA010000563.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 1894)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1894)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1894
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_77312_length_1894_cov_2.707993"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..542
                     /locus_tag="PLP95_12550"
     CDS             <1..542
                     /locus_tag="PLP95_12550"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPB46679.1"
                     /translation="MNLSRLDSGEMVLHLESTLVADLIDSAVNEFRWHHADDEDLEVE
                     VSVSPDDIHFEVDPLLISRVVTNLLENAFRHGAAPYAVAARQYPAAHGRPANVEIVVA
                     DHGPGIPPDEASRVFERFQRLDRSRRSGGSGLGLAIVSGIVERHHGTVGVDDNEPGGA
                     RMVVTLPQRQPNFEHRQAQ"
     gene            complement(554..1660)
                     /locus_tag="PLP95_12555"
     CDS             complement(554..1660)
                     /locus_tag="PLP95_12555"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HPB46680.1"
                     /translation="MNFNFTEEQQAIAELADQIIGDHSDPAALREIELGSGPRFDAGL
                     WSALAEAGLLGIAAPESAGGAGLGLVEAALVIRASALHAAAAPVWETLGLGIPVLSRY
                     VDNEVAAGWLAKAASGEAIVTAAWHEELGEPLDPQLVTATADGPAWSLSGTKVLVPAG
                     LIANAVIVPARTESGPALFLVDLSDLGDAERVEVRTTAATPDANLHFANTAATLLATG
                     DEAIAWAYDRAVATQCAAALGTAESALRITADYTKERKQFDVPIATFQAVGHRAADSY
                     IDVESIRLTTMQALWLLAADRDATIEVSAAQYWAAQSGQRVSLAAAHLHGGVGGDRDY
                     PLHRYFIFEKQYELHLGGASAALRRLGREIAASA"
     gene            complement(1686..>1894)
                     /locus_tag="PLP95_12560"
     CDS             complement(1686..>1894)
                     /locus_tag="PLP95_12560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020379203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HPB46681.1"
                     /translation="YLEAFQLLMEVLGPRSYLHRHSPAEAIKGRLEMMYRSLVILTYG
                     GGTNEIQRDLIAMFGLGLPRALRG"
BASE COUNT          312 a          624 c          643 g          315 t
ORIGIN      
        1 tgatgaacct gtcccgcctc gattccggtg agatggtgct gcacctcgaa tcgaccttgg
       61 tcgccgacct gatcgattct gcggtgaatg aattccgctg gcaccacgcg gacgatgagg
      121 acctcgaggt cgaggtatcg gtcagtcctg acgacatcca cttcgaggtc gatccgctgc
      181 tgatctcacg ggttgtgacc aacctgttgg agaacgcgtt tcgccacggc gcagctccgt
      241 acgcggtcgc tgcgaggcaa tacccggcgg cccatgggcg gcccgccaat gttgagatcg
      301 tcgtggccga tcacggcccg ggcatcccgc ccgacgaggc cagccgagtg ttcgaacgct
      361 ttcagcggct cgaccgctca cgtcgctcgg gcggctccgg gctcggcctg gccatcgtgt
      421 cgggcatcgt cgaacgccat cacggcaccg tcggtgtcga cgacaacgag cccggcggtg
      481 ctcgaatggt cgtgacgctt cctcagcgtc aacccaactt cgagcaccgc caggcccagt
      541 aacgacgccg tagctacgcg ctcgccgcga tctcgcggcc caatcggcga agcgccgcgc
      601 ttgccccgcc gagatgcaac tcgtactgct tttcgaagat gaaatagcgg tgcaaggggt
      661 agtcgcggtc gccgcccacg ccgccgtgga ggtgcgcggc ggcgagcgac acgcgttggc
      721 cggactgggc cgcccaatac tgtgcggcgg acacctcgat ggtggcgtcg cggtcggccg
      781 ccagcagcca caaggcctgc atggtggtga gccggatcga ctcgacatcg atgtaggagt
      841 cggcagcgcg gtggccgacg gcctggaacg tggcgatggg aacgtcgaac tgcttgcgtt
      901 ccttggtgta gtccgccgtg atccgcagcg cggattcggc cgtgccgagc gcggccgcgc
      961 actgggtggc aaccgcacgg tcataggccc acgcgatcgc ctcgtcgccg gtggccagca
     1021 acgtggccgc ggtgttggcg aaatgcaggt tggcatcggg ggttgccgcg gtggttcgca
     1081 cctcgacgcg ctctgcgtcg ccgagatccg acagatcgac gaggaacaac gccggtccgg
     1141 actcggtgcg tgccggcacg atgaccgcat tcgcgatcag cccggccggc accagcacct
     1201 tggtgccgct cagggaccag gcggggccat cggcagtcgc cgtgacgagt tgcgggtcga
     1261 gcggttcgcc gagttcctca tgccacgccg cggtgacgat cgcctccccc gaggcagcct
     1321 tcgcgagcca cccggccgcg acctcgttgt cgacatatcg cgagagcacc gggatgccga
     1381 gaccgagggt ttcccacacc ggcgcagccg cggcgtgcaa cgccgatgct cggatgacga
     1441 gcgccgcctc gacgaggccg agccccgctc ccccggcgga ttcgggggcg gcaataccca
     1501 acagtcccgc ctcggcaagc gccgaccaca gtccggcgtc gaagcggggt ccgctaccga
     1561 gttcgatctc acgcaacgct gcggggtcgg agtgatcgcc gatgatctga tcggcgagtt
     1621 ccgcgatggc ctgttgttct tcggtgaagt tgaagttcat ggtcgtcctt tgagaatccc
     1681 agcggtcagc cgcgaagtgc gcgcggtaga ccgagcccga acatggcgat gagatctcgc
     1741 tggatctcgt tggtgccgcc gccataggtg aggatcacca gcgagcgata catcatctcg
     1801 aggcggcctt tgatcgcctc ggccggcgag tggcggtgca gatacgagcg tgggccgagc
     1861 acctccatca acagttggaa cgcctcgaga taga
//
LOCUS       DAOOMA010000564         1891 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_77457_length_1891_cov_3.810458, whole genome shotgun sequence.
ACCESSION   DAOOMA010000564 DAOOMA010000000
VERSION     DAOOMA010000564.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 1891)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1891)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1891
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_77457_length_1891_cov_3.810458"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..464
                     /locus_tag="PLP95_12565"
     CDS             <1..464
                     /locus_tag="PLP95_12565"
                     /inference="COORDINATES: protein motif:HMM:NF012707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HPB46682.1"
                     /translation="YGFAVETGSELKPELDGALAEVRDDGTYDLIFNKWFPEDGNAGP
                     GAEDSDVEGSRTLKVCSDIPYEPMEFEGDGPRGLNYTGFDIELLDAMAVKIDANLEIL
                     AVPFDGILGNLASGTCDVVASSVTITEEREQEVDFTEPYFDADQSLLVKKD"
     gene            483..1370
                     /locus_tag="PLP95_12570"
     CDS             483..1370
                     /locus_tag="PLP95_12570"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HPB46683.1"
                     /translation="MPSDEQAEPTITMTDPSPHGAHFGEPEIYRSGRRKRVASTRRRL
                     AAYALGVAILVIVGLAADWDKLSTSFLNTEKFIDQFPEIVTIAAKNTVLYTVSSFIGG
                     VVLGTIMALLRLSSIRAYRWFAAVYIEVFRGLPALLTIVLVGFAVPIAFGLRFPSIFG
                     LPTGGIIGLSLVAGAYLAETLRAGIQAVPNGHIEAARSLGMSHGQTLRKVVLPQAFRI
                     VIPPLTNELVLLIKDTSLLSVLGVSVTSKEITKFARDASGQGWNYTPLLVGGLMYLMI
                     TIPLTRLVAYLEKRNERIR"
     gene            1367..>1891
                     /locus_tag="PLP95_12575"
     CDS             1367..>1891
                     /locus_tag="PLP95_12575"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HPB46684.1"
                     /translation="MTAAIQLLGLRKSFGDNEVLRGIDLSIEVGTVVCIIGASGSGKS
                     TLLRCVNRLEEPSGGSVLIDGVDITHPDTDLDLVRQRIGMVFQHFNLFPHLSVLGNVT
                     LAQKMVLKRSDAEAVDVATKMLERVGLADKVEAFPSQLSGGQQQRVAIARALAMNPPI
                     MIFDEATSALDPELV"
BASE COUNT          344 a          586 c          587 g          374 t
ORIGIN      
        1 agtacggctt cgcggtcgag accggctcgg aactgaagcc ggaactcgac ggtgcactcg
       61 ctgaggttcg cgacgacgga acctacgacc tgatcttcaa caagtggttc cccgaggacg
      121 gcaacgccgg gccgggggcc gaggactccg acgtcgaagg gtcgcggacg ctcaaggtgt
      181 gctccgacat cccctacgag ccgatggagt tcgaaggcga cggaccacgc ggtctgaact
      241 acaccggctt cgacatcgaa ctgttggacg cgatggccgt caagatcgac gcgaatcttg
      301 agatccttgc ggtgcccttc gacggcattc tcggcaacct ggcgtctgga acctgcgacg
      361 ttgtggcctc atcggtcacc atcaccgagg agcgtgagca ggaagtcgac ttcaccgagc
      421 cgtacttcga cgccgaccag tcgttgctgg tgaagaagga ctgatgttct ttcggaggtc
      481 acgtgccttc tgacgagcag gcagagccga cgatcaccat gaccgacccc agccctcacg
      541 gcgcgcattt cggcgagccg gagatctacc ggtccggacg acgcaaacgc gtcgcctcca
      601 cccgacgccg cttggccgcg tacgcgctcg gggtggccat cttggtgatc gtcggcctcg
      661 cggccgactg ggacaagttg tcgacgagct tcttgaacac ggagaagttc atcgatcagt
      721 tcccggaaat cgtcacgatc gcggccaaaa acaccgtctt gtacaccgtg tcgtcgttca
      781 tcggtggcgt ggtgttgggc acgatcatgg cgttgctgcg actcagttcc atccgggcct
      841 accggtggtt tgccgcggtc tacatcgagg tgttccgcgg ccttcctgcg ctcctcacga
      901 tcgtgctcgt cggcttcgcg gtgccgatcg cattcggtct ccggtttccg agcattttcg
      961 ggttgccgac gggaggcatc atcgggctgt cgctggtggc cggcgcctac ctcgcggaga
     1021 cgctgcgtgc gggcattcag gcggttccga acggccacat tgaagcagcg cgttcgctgg
     1081 ggatgagcca cggccaaacc ttgcgcaagg tcgtactgcc tcaagccttt cgcatcgtga
     1141 ttccaccgtt gacaaacgaa ctggtcctgt tgatcaagga cacctcgctg ctgtcggttc
     1201 tcggcgtgtc ggtgaccagt aaggagatca ccaagttcgc gcgtgacgca tccgggcagg
     1261 gctggaacta caccccgcta ctcgtgggtg gcctgatgta tctcatgatt accatcccgt
     1321 tgacgcgact cgtcgcctac ctcgagaagc gaaacgagcg gatccgatga ccgcggcgat
     1381 tcaactcctc ggcctacgaa agtcctttgg cgacaacgag gtgcttcggg gcatcgacct
     1441 gtcgatcgag gtgggcaccg tcgtgtgcat catcggcgcg tcgggttcgg gtaaatcgac
     1501 gctgcttcgc tgtgtgaacc ggctggaaga gccttcgggc ggatcggtgt tgatcgacgg
     1561 cgtcgacatc acacaccccg acaccgacct cgacctggtg cgtcaacgca tcggcatggt
     1621 ttttcagcat ttcaatctgt ttcctcatct cagcgtgctc ggcaacgtca cgctcgccca
     1681 gaagatggtg ttgaaacgat ccgacgccga agccgtcgac gtggcaacga agatgctgga
     1741 acgggtcgga ttggccgaca aggtcgaggc gttcccctcg cagctttccg gcgggcagca
     1801 acaacgggtc gcgatcgccc gggcacttgc gatgaatccg ccgatcatga tcttcgacga
     1861 ggcgaccagc gcgctcgacc ccgaactcgt c
//
LOCUS       DAOOMA010000565         1890 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_77531_length_1890_cov_2.518256, whole genome shotgun sequence.
ACCESSION   DAOOMA010000565 DAOOMA010000000
VERSION     DAOOMA010000565.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 1890)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1890)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1890
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_77531_length_1890_cov_2.518256"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(96..1646)
                     /locus_tag="PLP95_12580"
     CDS             complement(96..1646)
                     /locus_tag="PLP95_12580"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HPB46685.1"
                     /translation="MPDSLEFPSRFAIETPDHPAIINASDGRVVTYAQLDDRSLRIAN
                     LMRSLGLGVGDHMAVLVDNRPEFLEIVWAGLRSGLLVTPINWHLGADEAAFIIDDCEA
                     RVLFASGTVAEVGATIAASDSVGRLEGRFSVGGPIEGFVALDDAIVDQPTTPPSDECE
                     GNWMFYSSGTTARPKGVVPAFNGQPIGTANGFAMLVKFLYGGDETSVYLSPAPLYHAA
                     PAAWTTTMHRLGATSVVMDRVEPHAVLAAIEKYRVTHAQFVPTHLVRLLKLSEQERAR
                     YDLSSLRCVVHAAAPCPPEVKRAAMEWLGPIVYEYYSGSEGVGFCAIGPQEWLERPGS
                     VGKCLTGPVHIVGPDGEEVAVGEEGEVWFEPPGRFEYQGNEQATAQAYDHRGWSTLGE
                     MGRVDEEGYLYLTGRASNMIVSGGVNISPQESEDVLIAHPAVADVVVLGVPHPDFGEA
                     VRAIVQPVEGALARAGEEALGAELIEACRAQLSRYKGPTSVVFVEELPRLPTGKLVKR
                     KLPASLLR"
BASE COUNT          348 a          654 c          575 g          313 t
ORIGIN      
        1 gggcgttctg taggtaatcg cggttgtcat agcaaccgcg attacctaca gaacgccaga
       61 ggggggtaca gaacgccaga gggcgagagg gccggttagc gcaggagcga ggcgggcagt
      121 ttgcgtttga cgagcttgcc ggtaggcaac cgcggcaact cctcgacgaa cacgaccgag
      181 gtcgggccct tgtagcggct gagttgtgct cgacacgcct cgatgagttc ggcccccagc
      241 gcctcctccc cggcacgggc cagggcaccc tcgaccggct gcacgatcgc ccgaaccgcc
      301 tcgccgaaat ctggatgcgg aacgcccagc accaccacat ctgccaccgc cggatgggcg
      361 atcagcacgt cctcagactc ctgcggcgag atgttcaccc cacccgacac gatcatgttc
      421 gacgcccgcc cggtcagata gaggtagccc tcctcgtcga cgcgccccat ctctccaagg
      481 gtgctccaac cgcgatggtc ataggcctgc gccgtcgcct gctcgttgcc ctgatattcg
      541 aagcggccgg gtggttcgaa ccacacctcc ccctcctcgc cgaccgcgac ttcctcccca
      601 tcgggaccga cgatgtgcac cggccccgtg aggcacttgc ccaccgaacc cggacgttcg
      661 agccactcct gcggtccgat cgcgcagaac ccgacgccct cactgcccga gtaatactcg
      721 tacacgatcg gccccaacca ctccatcgcc gcacgtttca cctccggcgg acacggggcc
      781 gcggcgtgca cgacgcagcg cagactcgac aagtcatagc gcgcccgctc ctgttcggag
      841 agcttgagca gccgcaccag gtgggtcggt acgaactgtg cgtgggtcac ccggtacttc
      901 tcgatcgccg caagaaccgc gtgcggctcg acgcgatcca tcaccaccga ggtcgctccg
      961 agccgatgca tcgtggtggt ccatgccgcc ggcgccgcgt gatagagggg cgctgggctg
     1021 agatacaccg aggtctcatc gcccccgtag aggaacttca cgagcatcgc gaagccattc
     1081 gctgtgccga tgggttggcc gttgaacgcc gggaccacac ctttgggccg cgcggtggtg
     1141 cccgaggagt agaacatcca gttgccctcg cactcgtcgc tcggcggggt cgtcggctgg
     1201 tcgacgatcg catcgtccag tgcgacgaag ccctcgatcg gccctccgac gctgaatcgc
     1261 ccttcgagcc ggcccaccga gtcggacgcc gcgatcgtgg caccgacctc cgctaccgtc
     1321 ccagacgcga acagcactcg cgcctcgcag tcgtcgatga tgaacgccgc ctcgtccgct
     1381 cccagatgcc agttgatcgg cgtgaccaac agacccgacc gcaagccggc ccacacgatc
     1441 tcgagaaact ccggccgatt gtcaacgagc accgccatgt gatcgccgac gcccaagccg
     1501 agcgagcgca tgaggttggc gatccgcagc gagcggtcgt cgagttgcgc gtaggtgacc
     1561 acccggccgt cggaggcgtt gatgatcgcc ggatggtcgg gggtttcgat ggcgaatcgg
     1621 ctcgggaatt ccagcgagtc gggcatggtg gcactccgaa ggtggcggac ttcatacctc
     1681 acacggatgc gctgaggaaa aaccgagact atcgtgattc ggaaagtgac gcttttctca
     1741 tcgcgcaggc aactttccat cgcgtcaaca acgggggttg ccatgacggc cacgtcggtc
     1801 gagttcgatc cgttctcacc agagttcttc aacagtccgt acgagaccta tcgacgtctt
     1861 cgagacgagg caccgctgta cttcaacgag
//
LOCUS       DAOOMA010000566         1877 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_78322_length_1877_cov_3.832602, whole genome shotgun sequence.
ACCESSION   DAOOMA010000566 DAOOMA010000000
VERSION     DAOOMA010000566.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 1877)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1877)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1877
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_78322_length_1877_cov_3.832602"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..209)
                     /locus_tag="PLP95_12585"
     CDS             complement(<1..209)
                     /locus_tag="PLP95_12585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB46686.1"
                     /translation="MTTADAPVDLAPRRKPPIWVLPTGLITFTAVLVCGWVANSVWAN
                     WQDTHPLALIVLSPINRHLILTANQL"
     gene            290..1135
                     /locus_tag="PLP95_12590"
     CDS             290..1135
                     /locus_tag="PLP95_12590"
                     /inference="COORDINATES: protein motif:HMM:NF014193.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SURF1 family protein"
                     /protein_id="HPB46687.1"
                     /translation="MYRFLLKPRWLLSHVLVAAMVVGMLAAMMWQVSRLHQKQDLNAR
                     IEQRAHGEVLALGDVLAGHDLATDSGQDDVEYQAVSARGTYDTDHEFTIPNRTLDGAP
                     GRLVITPFVWSDTEAPILVNRGFIPQSFTDDTAPIDGVEPPEGEVAIAGYLRLTELPG
                     SLQTKRVDVGDNRFARLDLAAIAEVQGTALQPVYLLLGSQEPPTAQERLTMYPLPPRS
                     EGKHFSYAVQWAIFTLIACIGYPLVLRRIARNRAGQIDDDMPTEGMWHADARRRDDTV
                     EVGGE"
     gene            1132..1701
                     /locus_tag="PLP95_12595"
     CDS             1132..1701
                     /locus_tag="PLP95_12595"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HPB46688.1"
                     /translation="MRRSTARERIMDAVVACAERGGLGSFALEDVASQADVSRATIYS
                     HFPGGRQQLIDETVEREVADFWRSLADEVRSYDDVEDRVVAAMMSAHRRMIEHALLQR
                     LVTLEPGEILPALFAADRRLHGLIVGYLRETLAREVLRDGVDLDDAAEYLARMVLSHI
                     GTAGRWDLTDRTSVQRLVRTQFLGGILSR"
BASE COUNT          311 a          539 c          666 g          361 t
ORIGIN      
        1 agttggttgg cggtgaggat gagatggcgg ttgatcgggc tcaacacgat gagcgccagc
       61 ggatgtgtgt cctgccagtt cgcccaaacg gagttggcga cccacccgca caccaacacg
      121 gcggtgaagg tgatgaggcc ggtggggagc acccagatcg gaggtttgcg cctcggcgcg
      181 aggtcgacgg gtgcgtccgc agttgtcacg cgggtcagac tacgggaagt ctccgccttt
      241 gcggtccgcg gcggatgacc cgcgggacac ggcactagtt tggctggcga tgtatcggtt
      301 cttattgaag ccgcgctggt tgctgtccca tgtgctcgtc gcagcgatgg tggtcgggat
      361 gttggcagcg atgatgtggc aggtttctcg cttgcaccaa aagcaggatc tcaacgcgag
      421 gatcgaacag cgcgctcacg gcgaggtgct cgcgttgggt gacgtgttgg cgggccacga
      481 cctggcgacc gattccggcc aggacgatgt cgagtaccag gcggtttcgg cgagggggac
      541 ctacgacacc gaccacgagt tcacgatccc gaaccgcacg ctcgacggcg caccggggcg
      601 actggtcatc acccccttcg tatggtccga caccgaggct cccatcctgg tgaaccgagg
      661 gttcattccg cagtcgttca ccgacgacac ggctccgatc gatggggttg agccgccgga
      721 gggtgaggtg gcgatcgccg ggtatctgag gctcaccgag ttgccgggat cgttgcagac
      781 caagcgggtc gatgtcggcg acaaccgttt cgcccgcctc gaccttgccg cgatcgccga
      841 ggtgcagggc acggccttgc agccggtgta cctgttgttg ggttcccagg agccgccgac
      901 ggctcaggaa cggctcacga tgtatccgct tccgcctcgg tcggagggca agcacttctc
      961 ctatgcggtg caatgggcga ttttcacgtt gatcgcctgt atcgggtacc cgttggttct
     1021 gcggcgtatc gctcggaatc gggcgggtca gatcgacgac gacatgccga ccgagggcat
     1081 gtggcatgcc gatgctcgac gccgtgacga cacggtcgag gtcggcggcg aatgaggcgg
     1141 tcgaccgccc gcgaacgcat catggacgcg gtcgtcgcct gcgccgagcg aggtgggctt
     1201 gggtcgttcg ccctcgaaga cgttgcgtcg caggccgatg tgtcccgggc gacgatctac
     1261 agtcattttc ctgggggccg ccagcagttg atcgacgaaa cggtcgagcg cgaggtggcc
     1321 gacttctggc ggagcctcgc ggatgaggtc cgctcgtatg acgacgtcga ggatcgcgtt
     1381 gtcgcggcga tgatgtcggc gcaccgtcgg atgatcgaac atgcgctgtt gcagcgcctc
     1441 gtcaccttgg aaccgggtga gattctgccg gcgttgttcg cagcggaccg gcgcctccat
     1501 ggcctcatcg tgggatacct cagggagacg ttggcccgtg aggtcttgcg cgacggagtc
     1561 gacctcgatg atgcggcgga gtatctggcg cgcatggtgt tgagccacat cggcacggct
     1621 ggtcgttggg acctgaccga tcggacgtcg gtgcagcggt tggtccgcac ccagttcctc
     1681 gggggaatcc tctcccgata atgtgaaata tgtcacacgg tgcacaatga gacagattca
     1741 gtcgtattgt ctcatgtgtg accgatggca tgaacgtcga gttccccgca ggcggtgacg
     1801 ccggccaggg agtcgaggac cgggtgctcg acgctgcgct tgagtgcatc ggccggtggg
     1861 gtatgaccaa gacgacg
//
LOCUS       DAOOMA010000567         1874 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_78529_length_1874_cov_3.507422, whole genome shotgun sequence.
ACCESSION   DAOOMA010000567 DAOOMA010000000
VERSION     DAOOMA010000567.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 1874)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1874)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1874
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_78529_length_1874_cov_3.507422"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..469
                     /locus_tag="PLP95_12600"
     CDS             <1..469
                     /locus_tag="PLP95_12600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB46689.1"
                     /translation="YATTNDPQSPKVSGPLTSGATAYSSCAGCHGQTGGGGAGPALTN
                     VDELFPDPASHVRWVLLGTAGYQSEGTPTYGADNKPVGGAGNMPSQVALDSSELLSII
                     RHERETLAGETYDEQAWLDAVEELLNDPNPEVSSKAEEYGAIVESWANFAPGS"
     gene            466..1005
                     /locus_tag="PLP95_12605"
     CDS             466..1005
                     /locus_tag="PLP95_12605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB46690.1"
                     /translation="MTRSHKFIIVLTAAVLYGGSACSGDDSSNGTTVQATQEAAPSMA
                     DVTRDAGLVFPPSVSDFRLVNISETQIDLSFQIDADEVATFAEESGLTLTRGERAIKH
                     ASPIWQLEVYGEFSGSVTNFGKVQRAVEVVANEAFVAPSTTAGPTTTRADSDAPTNEM
                     PEIARDPGRYTVRATLTLG"
     gene            complement(1028..>1874)
                     /locus_tag="PLP95_12610"
     CDS             complement(1028..>1874)
                     /locus_tag="PLP95_12610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB46691.1"
                     /translation="AEGAGGAEASIAVLLAALLGGAAMHGRELLTIALDPRIESFALW
                     LEQLVAESSGKHGTGITPVVGEAIDVVAATPDRRIVVTIGEVAGIDRLREMDVPLVEL
                     GLEELTDIGAQVVLWEYAVALACRVLGVNPFDQPDVESAKVAARALLDRAAPANPVEL
                     SLEEGLALAVPGDTIALCGFVDPGGADADALEALRSRLGRELGFVTTLGFGPRFLHST
                     GQLHKGGPSEIVVLQFLRSAEELAVPGSALGFEQIKAAQAAGDLVALAEAEQRCARIR
                     LDDLA"
BASE COUNT          328 a          717 c          574 g          255 t
ORIGIN      
        1 ctacgcgacc accaacgatc cgcaatcccc caaggtctcc gggccgctca ccagcggcgc
       61 cacggcgtac tcctcctgtg caggctgtca cggccaaacc ggcggcggcg gcgcgggccc
      121 ggcgctcacc aacgtcgacg agctgttccc cgatccggca tcgcacgtgc gttgggtcct
      181 gctcggcacc gccggctatc agtccgaggg cacaccgacc tacggcgccg acaacaagcc
      241 cgtcggcggc gcaggcaaca tgccctcgca ggtcgcgctc gactccagcg aactgctgtc
      301 gatcatccgc cacgagcgcg agacgctcgc cggcgagacc tacgacgagc aggcatggct
      361 cgacgcggtc gaggaactcc tgaacgaccc gaatcccgag gtcagctcca aggcggagga
      421 atacggagcc atcgtcgaaa gctgggccaa cttcgcgccg ggttcgtgac ccgctcgcac
      481 aagttcatca tcgttctcac tgccgccgtc ctgtacggcg gcagtgcctg ttccggcgac
      541 gactcgtcca acggcaccac ggttcaagcc acccaggagg cggcgccgtc gatggcggat
      601 gtcacccgcg atgccgggct ggtgttcccg ccgtcggtca gcgattttcg cctcgtcaac
      661 atctcagaga cccagatcga cctgtccttt cagatcgatg ccgacgaggt ggccacgttc
      721 gccgaggaat cagggttgac cctcaccaga ggtgagcggg ccatcaagca cgcctcaccg
      781 atctggcagc tcgaggtgta cggcgagttc tcggggtcgg tcaccaactt cgggaaggtg
      841 caacgggcgg tcgaagtcgt ggccaacgaa gccttcgtcg cgccgagcac caccgccggg
      901 ccgaccacga cgcgcgccga cagcgacgcc ccgaccaacg agatgcccga gatcgcccgc
      961 gaccccgggc gttacaccgt tcgggccacc ctcaccctcg gctgacccgc ctacccgcct
     1021 gcccgggcta cgccaggtcg tcgaggcgga tgcgggcgca gcgctgctcc gcctcggcca
     1081 gcgccaccag atcgccggcg gcctgtgccg ccttgatctg ctcgaaaccc aacgcgctgc
     1141 ccggtaccgc caactcctcg gcgctacgca gaaactgcaa cacgacgatc tccgacggcc
     1201 cgcccttgtg caactgcccg gtcgaatgca ggaaccgcgg gccgaacccc agcgtggtca
     1261 caaacccgag ttcgcggccc aaccgcgagc ggagggcttc gagcgcgtcg gcgtccgcac
     1321 caccgggatc gacaaacccg cacagtgcga tcgtgtcgcc gggcaccgcc aacgccaagc
     1381 cctcctccag tgacaactcc accgggttcg ccggcgcggc acggtcgagc agggcgcggg
     1441 ccgcaacctt cgcgctctcg acatcgggct gatcgaacgg attcaccccg agcacccggc
     1501 acgccagcgc aaccgcgtat tcccacagca ccacctgggc gccgatgtcg gtcagctcct
     1561 cgagccccaa ctcgacgagc ggaacgtcca tttcccgcag ccggtcgata cctgcgacct
     1621 cgccgatcgt cacgacgatg cggcgatccg gggtcgccgc caccacgtcg atcgcctcgc
     1681 ccaccaccgg ggtgatgccg gtgccgtgtt tgccgctcga ctcggcgacg agttgctcga
     1741 gccacagcgc gaacgactcg atgcgagggt cgagcgcgat ggtcaacaat tcgcggccat
     1801 gcatcgccgc gccgccgagc agcgcggcga gcagcaccgc gatcgacgcc tcggcaccgc
     1861 cagcgccctc cgca
//
LOCUS       DAOOMA010000568         1872 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_78633_length_1872_cov_4.418272, whole genome shotgun sequence.
ACCESSION   DAOOMA010000568 DAOOMA010000000
VERSION     DAOOMA010000568.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 1872)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1872)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1872
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_78633_length_1872_cov_4.418272"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..727
                     /locus_tag="PLP95_12615"
     CDS             <1..727
                     /locus_tag="PLP95_12615"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020045332.1"
                     /note="Produces ATP from ADP in the presence of a proton
                     gradient across the membrane. The beta chain is a
                     regulatory subunit; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HPB46692.1"
                     /translation="LTMAEYFRDVEGQDVLLFIDNIFRFTQAGSEVSTLLGRMPSAVG
                     YQPTLADEMGALQERITSTGGRSITSLQAVYVPADDYTDPAPFTSFTHFDGTTELSRD
                     IAALGIYPAVDPLASTSTILAPEVVGDRHYGVARRTQEVLQRYKELQDIIAILGLDEL
                     SEEDRITVDRARKVQKFLAQPMFVAEVFTGLPGIFTPVSETIESFEALLNGDLDHLPE
                     QAFYNVGGAEDVLRKAKELEKNA"
     gene            727..1137
                     /locus_tag="PLP95_12620"
     CDS             727..1137
                     /locus_tag="PLP95_12620"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HPB46693.1"
                     /translation="MALQVELVSPERIAYSGEAKEVIARVVTGDIAFLTGHVPYLGIL
                     QSAPVRVLFEDGTELPIAVHQGFVQVADDTVTILSDVCELPDQIDIRRAEAAKAKAEE
                     ALRADVGDGEAQDALRRAQTRISVATGVVAGARH"
     gene            complement(1264..>1872)
                     /locus_tag="PLP95_12625"
     CDS             complement(1264..>1872)
                     /locus_tag="PLP95_12625"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012222641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphatase class II"
                     /protein_id="HPB46694.1"
                     /translation="PGPCVYMDKIATGPEAAEVIDINKSVAENLAAVAAAKGKKVSEL
                     TAVILDRPRHEQIIAEVRAAGTRIRLIPDGDVAGAISTAWPESGADIMFGVGGTPEGV
                     IAAAALKCMGGAIQGKLWPRNDEERQAAIEAGYDLDEVLTEETLVAGNNCFFSATGIT
                     DGELLEGVRYNRAGATTQSLVMRSTSGTVRLVEAQHRAAKLG"
BASE COUNT          302 a          614 c          621 g          335 t
ORIGIN      
        1 gctcacgatg gcggagtact tccgcgatgt ggaaggccag gacgtgctgt tgttcatcga
       61 caacatcttc cgcttcaccc aggcgggttc cgaggtgtcg accctgctcg gtcgtatgcc
      121 ctcggcggtg ggttaccagc cgaccctcgc cgatgagatg ggtgcgcttc aggagcggat
      181 cacctcgacc ggcggtcgct cgatcacctc gctgcaggcc gtgtacgtgc ccgccgacga
      241 ctacaccgat ccggcgccgt tcacctcctt cacccacttc gacggcacca ccgagttgag
      301 ccgtgacatc gcggcgctcg gcatctaccc ggcggtggat ccgctggcgt cgacctcgac
      361 gattctcgcc cccgaggtcg tgggcgaccg tcactacggc gttgcccgcc gcactcagga
      421 agtgctgcag cgctacaagg aactccagga catcatcgcc attctcggcc tcgatgagct
      481 ctccgaggaa gaccgcatca ccgtcgaccg tgctcgtaag gtgcagaagt tcctggcgca
      541 gccgatgttc gtcgccgagg tgttcaccgg tctgcccggt atcttcacgc cggtgtcgga
      601 gacgatcgag tccttcgaag cgctgctcaa cggcgacctc gaccacctgc ccgagcaggc
      661 gttctacaac gtcggtggtg ccgaggacgt gttgcgcaag gcgaaggaac tggagaagaa
      721 cgcctaatgg cgctgcaggt cgaactcgtc tcacccgagc gcatcgcgta ctcgggcgaa
      781 gccaaagagg tgatcgcacg tgtcgtcacc ggtgacatcg cgtttctgac cgggcacgtg
      841 ccgtatctgg gcatcttgca gtcggcaccg gtgcgcgtgt tgttcgagga cggcaccgag
      901 ttgccgatcg ccgttcacca gggcttcgtc caggtggccg acgacacggt cacgatcctg
      961 agcgacgtct gcgagctgcc cgaccagatc gacatccgtc gtgccgaggc ggccaaggcg
     1021 aaggccgagg aggcgttgcg cgcggatgtg ggcgacggtg aggctcagga cgcgctgcgt
     1081 cgggcccaga cccgtatttc ggtggcgacc ggggtcgtcg ccggcgctcg ccactgacgc
     1141 gcacccggcg cagcgccggt cacccgacgc accgccggtc acccaacgac ccaacgaacg
     1201 gttctgtagg tagtttctgt cgctctggca acagaaacta cctacagaac gttttcggtc
     1261 aggtcagccg agcttggcgg cgcggtgttg ggcctcgacg aggcgcacgg tgcccgaggt
     1321 cgagcgcatg acgagcgact gcgtcgtcgc cccggcgcgg ttgtagcgaa ccccttcgag
     1381 gagttcgccg tcggtgatgc cggtggcgct gaagaagcag ttgttgccgg cgacgagggt
     1441 ctcctcggtg aggacctcgt cgaggtcgta gccggcctcg atcgcggcct gtcgttcctc
     1501 gtcgttacgc ggccagagct tgccttggat ggcgccgccc atgcacttga gcgcggccgc
     1561 ggcgatgacc ccttccggag tgccaccgac gccgaacatg atgtcggcac ccgattccgg
     1621 ccatgccgtg gagatggcac ccgcgacgtc gccgtcgggg atgagccgga ttcgggtgcc
     1681 cgcagcgcga acctcggcga tgatctgttc gtggcgggga cgatcgagga tgaccgcggt
     1741 caactccgaa accttcttgc ccttggccgc agcgaccgca gcaaggttct cggcgaccga
     1801 cttgttgatg tcgatgacct cggcggcctc ggggccggtg gcgatcttgt ccatgtacac
     1861 acacggaccc gg
//
LOCUS       DAOOMA010000569         1866 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_79055_length_1866_cov_3.652678, whole genome shotgun sequence.
ACCESSION   DAOOMA010000569 DAOOMA010000000
VERSION     DAOOMA010000569.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 1866)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1866)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1866
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_79055_length_1866_cov_3.652678"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            186..476
                     /locus_tag="PLP95_12630"
     CDS             186..476
                     /locus_tag="PLP95_12630"
                     /inference="COORDINATES: protein motif:HMM:NF014643.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system
                     prevent-host-death family antitoxin"
                     /protein_id="HPB46695.1"
                     /translation="MSTVSATEARTTLPDLLDRVTSGEEITITRHGRTVAVLVHPDAL
                     RVRKAGRALAEAEQVGALLRRARELPLADMPSISTDRADELIAELAATRSIR"
     gene            484..900
                     /locus_tag="PLP95_12635"
     CDS             484..900
                     /locus_tag="PLP95_12635"
                     /inference="COORDINATES: protein motif:HMM:NF013970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HPB46696.1"
                     /translation="MDAFDAEALIYAAVPNHPLGKRVRRLLSAAATSPDGTGIGSVLL
                     IPELLSKPMREGATTEMTELATLLGRLILLPVDEHVAGLAAALGSSYRLRAADAVHLA
                     TAVAAGADRFITNNQSDFPTTISEIEIVYPAELPHP"
     gene            complement(948..1595)
                     /locus_tag="PLP95_12640"
     CDS             complement(948..1595)
                     /locus_tag="PLP95_12640"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HPB46697.1"
                     /translation="MAKIETIDQGAAGPRSRKGAQTRARLLEAAKAVFERTGFLEARV
                     SDIAKEAGMSHGSFYHYFDSKEQVFREVAEQQEALLTAPPSESETDDGGAELSEFDRL
                     LRANRRYLERYRDNGRIMGVIEEVSRYDHEVSEARMRRQKHFADRAERSIRRMQRSGA
                     VSKALDPEIAAGALGSMVARFAELWLIEHWGDYDLDEVAMQLMLLWANAIGLDTP"
     gene            complement(1681..>1866)
                     /locus_tag="PLP95_12645"
     CDS             complement(1681..>1866)
                     /locus_tag="PLP95_12645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB46698.1"
                     /translation="VPSFVRVDVELPKLSSMKIDKKALRAQAWEAQRVYWRPSRNEGL
                     RLLDADGTVGLAPLLRR"
BASE COUNT          355 a          666 c          526 g          319 t
ORIGIN      
        1 cccacgaatc gaccccaccc caccaacaac aagccaaccc ggcacaatta cccacgcccc
       61 cagacatcgg gtcggagtcc accccgggag gtaggcacag cgatacgagg gcccgagaca
      121 ccaccacgca aaggggactc agactgctga ttgtatggag ccgtacgttg atgtacattc
      181 aacccatgag caccgtatcg gcgacagaag cccgaacgac gttgcccgat ctcctcgacc
      241 gggtgacatc aggtgaggaa atcacgatca cccgccacgg gcgaacagtc gctgtactcg
      301 tacaccccga cgcgctgcgt gtccgcaagg cggggcgcgc gcttgcggag gccgagcagg
      361 tcggcgcgct gctccgacgt gcacgcgaac tccccctcgc ggacatgccc tcgatctcga
      421 ccgatcgcgc cgacgaactc atcgccgagt tggcagcaac acgatcgatt cgttgagtcg
      481 gcaatggacg cattcgacgc cgaagcattg atctacgcag ccgtgccgaa tcatccgctc
      541 ggcaaacgtg tgcgccgtct cctctcagca gccgcgacct ccccggacgg cactggcatc
      601 ggatcggtgc tcctcatccc cgaactcctc tccaagccga tgcgcgaggg agcgacaacc
      661 gagatgacgg aactggccac gttgctcggg cggctcattc tcctacccgt tgacgaacat
      721 gtcgccggtc tggctgccgc actgggatcc agttatcggc tacgcgctgc ggacgcggtg
      781 catctggcga ctgctgtcgc cgcaggcgcc gaccgtttca tcaccaacaa tcagtccgat
      841 tttccaacca ccatctcaga gatcgagatc gtctacccgg ccgagctgcc acacccttga
      901 ccgagcacta gtcgcgactg gattcagcga ccgtcggtga tgccgcacta cggggtatcg
      961 aggccgatcg cgttggccca caacagcatg agttgcatgg caacctcgtc gaggtcgtag
     1021 tcgccccagt gctcgatcaa ccacagttct gcaaacctcg ccaccatcga gcccagcgct
     1081 cctgccgcga tctccgggtc gagagctttc gacaccgctc cagatcgctg catccgacga
     1141 atcgaacgct cggcgcgatc cgcgaagtgc ttctggcgac gcatccgcgc ctcactcact
     1201 tcatggtcgt accgcgacac ctcctcgatc acccccatga tccggccgtt atcgcggtac
     1261 cgctcgaggt agcgacgatt cgcccgcaac aaccggtcga actcgctgag ctcggccccg
     1321 ccgtcgtccg tttccgattc cgacggcggg gcggtcaaca gcgcctcttg ctgctcggcc
     1381 acctcgcgaa acacctgctc cttggaatcg aagtagtggt agaaggaccc gtgcgacatc
     1441 cccgcttcct tggcgatgtc ggagacccgg gcctcgagaa acccggtgcg ttcgaacacc
     1501 gccttcgctg cctcgagcag acgtgcacgg gtctgtgccc ccttgcggga acgcggtccc
     1561 gcggcaccct ggtcgatggt ctcgatcttc gccataggca gcactctctc gatgggtgag
     1621 ctcggtcgac tcggtcgccg tcgattctac ggggatgggt ccgcttcgac gtcgaagcgc
     1681 tcagcgacgc agcagcggcg caaggccgac ggtgccgtcg gcatcgagca accgaagccc
     1741 ctcgttgcgt gacggacgcc agtacacccg ctgcgcctcc cacgcctggg cccgcagcgc
     1801 cttcttgtcg atcttcatac tcgacagctt cggcagttca acatcaactc gaacgaagga
     1861 cggcac
//
LOCUS       DAOOMA010000570         1862 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_79318_length_1862_cov_2.740454, whole genome shotgun sequence.
ACCESSION   DAOOMA010000570 DAOOMA010000000
VERSION     DAOOMA010000570.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 1862)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1862)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1862
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_79318_length_1862_cov_2.740454"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            50..310
                     /locus_tag="PLP95_12650"
     CDS             50..310
                     /locus_tag="PLP95_12650"
                     /inference="COORDINATES: protein motif:HMM:TIGR01764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HPB46699.1"
                     /translation="MKKLLPDPKSQPTLSVDEVAEILGLDRKTVYASVRSGELPSLRV
                     GRRILVPTRWLAARIAPDADEAALTGSRSPRSQHPAQGQLPL"
     gene            327..1544
                     /locus_tag="PLP95_12655"
     CDS             327..1544
                     /locus_tag="PLP95_12655"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HPB46700.1"
                     /translation="MNGWITQRRGTYYVVIDHGADRLTGKRQRRWHRAGTTKRAAQHL
                     LATLLTEHETNRHVEADEMTLRVYLTDEWLPSVRNELRHSTYESYRRNLVNHVLPHLG
                     DARLQMIRPIDLTRFYTGLLTDGRRNGAGGLSAKSVRNVHVALHKAFDDAVDLLYLRS
                     NPAATAKPPRARATDTDSIRYWTGPELRHFLDTNQDHHHWPIWYLAANTGMRRGELLG
                     LRWRDVDFDGRCLSVRRAIISVAGEVIESQPKTRRGQRTVDLDSHTVEVLASHRRSQA
                     EQRRVLGLPGSAELVFTKPDGTNFHPDNVRQAFERRVLRTEVPRIRFHDLRHTHATLL
                     LKAGAPPKVVSERLGHATVAFTMDVYAHVIPGMQAEAAQLFHDLVFAVDEPVQDGTEN
                     GPAESDDSASRED"
BASE COUNT          345 a          642 c          568 g          307 t
ORIGIN      
        1 aagcgcaact gaatacgacc ggtcgcaacc cgtgcgcgct taggtgggta tgaagaagtt
       61 gctgccagat ccgaaatctc aacccacgtt gagcgtcgac gaggtcgccg agatcctcgg
      121 cctcgaccgg aagaccgtct acgcgtcggt acggtccggt gagctaccgt cgctgcgggt
      181 cggccgacga atcctcgttc cgacccgatg gctcgccgct cgcatcgcac ccgatgccga
      241 cgaagctgct ctcaccggtt cccggtctcc ccgatcgcag catcccgccc agggtcagct
      301 gcctctctga ccaggagacc agtccgatga acggatggat cacccaacgt cgcggcacct
      361 actacgtcgt gatcgaccac ggcgccgatc gattgactgg caagcggcag cgacgctggc
      421 atcgagccgg gacgacgaaa cgcgctgccc aacatctgct ggcgacgctg ctcactgagc
      481 acgagacgaa ccgtcacgtc gaagccgacg agatgacact ccgggtgtac ctgacggacg
      541 agtggttgcc ctcggtgcgc aatgagctac ggcactccac gtacgagtcc tatcgccgca
      601 acctggtcaa ccatgtcctt ccccacctcg gggacgcccg cctccagatg atccgaccga
      661 tcgacctgac ccgcttctac accggcctgc tgactgacgg tcgccgcaac ggagcgggcg
      721 gcctgtcggc caaatcggtc cgcaacgtcc acgtcgcgct acacaaggcg ttcgacgacg
      781 ccgtggacct gctgtacctg cggagcaacc ctgccgcgac cgccaagcct cccagggctc
      841 gagcaaccga caccgactcg atccggtact ggaccgggcc cgagctccgg cactttctcg
      901 acacgaacca ggatcaccac cactggccga tctggtacct cgcggcgaac acggggatgc
      961 gacgtggcga gctgctcggg ttgcggtggc gcgacgtcga cttcgacgga cggtgcctgt
     1021 cggttcgccg ggcgatcatc tccgtcgccg gcgaggtcat cgaatcccaa cccaagactc
     1081 gacgtggcca gcgcacggtc gacctcgaca gccacactgt cgaagtgctc gcttcacaca
     1141 ggcgctcaca ggctgagcaa cgacgcgtcc tcggtcttcc tggctccgcc gagcttgtgt
     1201 tcaccaagcc ggacgggacg aacttccacc ccgacaacgt tcggcaggcg ttcgaacggc
     1261 gggtgctccg cacggaggtt ccgcgcatcc ggttccacga tctgcgccac acccacgcca
     1321 ccctgcttct caaggccggg gcgccgccga aggtcgtgtc cgagcgcctc ggccatgcga
     1381 ccgtggcgtt cacgatggac gtctacgcgc atgtcatccc cggcatgcag gccgaagcag
     1441 cacagctgtt ccatgacctc gtgttcgcag tcgacgagcc ggttcaggac ggcacggaga
     1501 acggacccgc cgagagcgac gactccgcct cgcgggagga ctgatcggcc gatcagcgtc
     1561 gtggtcagcc accggcctcg atgaccttcg ggttttcggg cagccgctcg gcgacacgat
     1621 ttggcagatc tggcagacaa tctggcagac acgggtcgat cggctaaccc cgggaaccac
     1681 aacggcgggc tgaaaagccc gccgttactg gattctggat ggtggcgggg gcgggatttg
     1741 aacccgcgac cttcgggtta tgagttagac ggtcaaccgt tcaacccgta ccagcaagtg
     1801 tcgaaacatc ccattctgtg gggatttcgg cgttgccaac ggacgtctcg tgccgttggg
     1861 tg
//
LOCUS       DAOOMA010000571         1858 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_79575_length_1858_cov_3.333333, whole genome shotgun sequence.
ACCESSION   DAOOMA010000571 DAOOMA010000000
VERSION     DAOOMA010000571.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 1858)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1858)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1858
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_79575_length_1858_cov_3.333333"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..189)
                     /locus_tag="PLP95_12660"
     CDS             complement(<1..189)
                     /locus_tag="PLP95_12660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB46701.1"
                     /translation="MGKRRQQQFEESGVEDDLLVAIQRLGRLMGSRQVAGRIADAADV
                     EIPQQGVQILRSLLRHSEL"
     gene            complement(286..>1858)
                     /locus_tag="PLP95_12665"
     CDS             complement(286..>1858)
                     /locus_tag="PLP95_12665"
                     /inference="COORDINATES: protein motif:HMM:NF013530.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="nitroreductase family deazaflavin-dependent
                     oxidoreductase"
                     /protein_id="HPB46702.1"
                     /translation="AGHSVVALVRSVDRLIEPMAALGVAVPETVIGDMNDSEAVRSAL
                     STCDAVVHCADVHCADVHCDAEVSTDPRSDGLMIERNRNGTRTVLGLAHELGLDPIIH
                     ISSTSALFEPGVAALTPDHPIGRSSMPHARSMPYARSAAACEKVARDLQADGAPVVIV
                     YPSGILGPGPGPGPGPGPGPGPGAALGEPATQMAHLVARFVASGVMPTRHAALSIIDV
                     RDLAAVIVRLLEPDRGVRRIMCGGHFVGLATLAAELRRLTGRRFPVAPVPPAALRWAG
                     SVTDAVRGRFNFETASTYEAMALATRWNGTDDTSMTDLGIELRPLGDTLAASLSAWSE
                     AGLVTSRQLGAFEPAMGRPARPPRGIRVPGRVMSSRPLRKFGPRVFPPLHRAIDRLSG
                     GRIIGDSRASPLMILEHTGAKTLTVRRTPLAVVPRSCGTFLALGSNFAQESHPAWTTN
                     LLAHPSTTMHFRGRDIAVEAVLLTGQERADAWDEAITWYPGWVRYAQFTDREFRLFEL
                     VPEGHERHPTNRCEP"
BASE COUNT          326 a          613 c          638 g          281 t
ORIGIN      
        1 caactcactg tgacgcagca acgagcggag aatctgcaca ccctgttggg ggatctcgac
       61 gtcagcagca tcagcgatcc gacccgcgac ctgacggctt cccatcaggc gacccaggcg
      121 ctggatcgca acaaggaggt cgtcctcaac ccccgactcc tcaaactgtt gttgcctacg
      181 cttgcccacg gatgctcctc gcccggccga gtgcgatcgt acggaacctg tccggacccg
      241 ctaacacccc cgagtccgcg cagcggcgag tcaacgcagc gccgatcacg gttcacagcg
      301 gttggtcgga tgtcgttcgt ggccctcggg cacgagttcg aagagccgaa actcccggtc
      361 ggtgaactgc gcgtatcgca cccaccccgg ataccacgtg atggcctcgt cccaggcgtc
      421 ggcacgttcc tgacccgtca agagcacagc ctcgaccgcg atgtcgcgtc ctcgaaagtg
      481 catcgtcgtc gaggggtgag ccagcaggtt ggtcgtccac gccgggtggc tttcttgcgc
      541 gaagttgctg ccgagagcca gaaacgttcc acaggaacgt gggaccaccg ccagcggtgt
      601 gcggcgaacg gtcagcgtct tggctccggt gtgttcgagg atcatcaacg gcgaggctcg
      661 actgtcgccg atgatgcgac cgccgctgag tcggtcgatc gcccggtgaa gcgggggaaa
      721 gaccctcggc ccgaacttgc gcagcggccg agagctcatc accctgcccg ggacgcgaat
      781 gccgcgcggc gggcgtgccg gcctgcccat tgccggttcg aacgctccga gctgacgaga
      841 cgtgaccagg ccggcctccg accaggctga caacgacgca gcgagggtgt ctccgagcgg
      901 gcgtagctcg atgccgaggt cggtcatcga ggtgtcgtcg gttccgttcc accgggtcgc
      961 caacgccatc gcttcgtagg tcgatgcggt ctcgaaattg aaccgccctc gcaccgcgtc
     1021 ggtcaccgac ccggcccacc gcaaggctgc gggaggcacc ggggcgaccg gaaagcggcg
     1081 gccggtgagt cgtcgcaact cggcggcgag ggtggcgaga ccgacgaaat ggcctccgca
     1141 catgatccgg cgaacacccc ggtccggttc gagaagccga acgatgaccg cggcgagatc
     1201 tcggacgtcg atgatcgaaa gtgcggcgtg acgggtaggc atcacgccgc tggcaacgaa
     1261 gcgagcaacc aagtgagcca tctgggtggc tggttcgccg agcgccgcgc cggggccggg
     1321 gccgggaccg gggccgggac cggggccggg gccggggccg agaattccgc tcgggtacac
     1381 gatcacgacc ggggcaccgt cggcttggag gtcgcgagcc accttttcgc acgccgccgc
     1441 cgatcgtgcg tacggcatcg atcgtgcgtg cggcatcgac gaccggccga tgggatggtc
     1501 gggagtcaac gctgcgaccc cgggctcgaa cagggcgctg gtgctcgaga tgtgaatgat
     1561 ggggtcgagc ccgagctcat gggcgaggcc gagcacggta cgggtgccgt tgcggttccg
     1621 ttcgatcatc aacccgtcgc tacgaggatc cgtcgacacc tcggcgtcgc agtggacatc
     1681 ggcgcagtgg acatcggcgc agtgaacaac ggcgtcgcag gtgctcaacg cggaacgaac
     1741 cgcttcgctg tcgttcatgt ccccgatcac cgtctcggga acggcgacac cgagcgccgc
     1801 catcggctcg atcaatcggt cgacgctgcg aacgagcgcg acgacgctat ggccggcg
//
LOCUS       DAOOMA010000572         1857 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_79668_length_1857_cov_2.344617, whole genome shotgun sequence.
ACCESSION   DAOOMA010000572 DAOOMA010000000
VERSION     DAOOMA010000572.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 1857)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1857)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1857
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_79668_length_1857_cov_2.344617"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..388)
                     /locus_tag="PLP95_12670"
     CDS             complement(<1..388)
                     /locus_tag="PLP95_12670"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HPB46703.1"
                     /translation="MTDDVTTSGAGRPNQREFILDTALQLIATNGAAKMSMRQLAKQC
                     GLNVAAIYHYFDSKDALVKAVVDERQYGARMAEIVEVDASLSAEDRLFAVFMVIWDGA
                     IAEEEVWRILLGEGLRGEPSVMVVGRD"
     gene            complement(488..1816)
                     /locus_tag="PLP95_12675"
     CDS             complement(488..1816)
                     /locus_tag="PLP95_12675"
                     /inference="COORDINATES: protein motif:HMM:NF012296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HPB46704.1"
                     /translation="MTSPVFTPFSPTRDRQPVDLDIVDFTDPSTFDDPWDTYAALRDL
                     DHLHYDAKNDLYIAPRHEDVFHISRDNELYVSRFGVRPIIAGDMSIITMDGPDHVATR
                     RLINKGFTPRRVRAMMPHIRELAGQLIDDVIDSGHIDFVEQFAIHVPLIIICELMGLD
                     PAQRMDMYRWSDQMMAGDGHVDGDDPVLLAAAEAFGEFAVLCMGLIAERREDPKDDLI
                     SILTKAYDEGGLEREHEKVYQGVDDATRQAMLEKSALNDDELFAFLAVLMVAGNETTR
                     NAITGGLLALSEYPEQKQLMIDNLFNDEFMDPAIEELIRYVTPVLGFVRTVEQDHTYR
                     NTDLKKGDRVLMLYAAANRDERAISNPDVLDFQRPENPHMAFGIGPHYCLGANLARME
                     VKLVFQELLSRMPDITVPAGAKVPRGESSLVLALQHMPADFTAGGCPVAH"
BASE COUNT          295 a          544 c          676 g          342 t
ORIGIN      
        1 ggtcgcggcc gaccaccatg accgagggct cgccacgaag gccttcgccg aggaggattc
       61 gccagacctc ctcctcggcg atcgctccgt cccagatgac catgaacacc gcgaacagcc
      121 ggtcctcggc cgacagcgac gcgtcgacct cgacgatctc ggccatgcgg gccccgtact
      181 ggcgctcgtc gacgaccgcc ttcaccaagg catccttgct gtcgaagtag tgatagatcg
      241 ccgcgacgtt gaggccgcat tgcttggcca actggcgcat cgacatcttc gccgcaccgt
      301 tggtggcgat gagctgcaac gcggtgtcga ggatgaactc gcgctggttc ggccgtccgg
      361 ctccggaggt tgtcacgtcg tcagtcatgg tgcggtggct ctcatgcggt ccgtttgtcg
      421 ggcaaaggtg cggcccctgc ccggccccgg gtgggtgggg caggggccgg gcaggggccg
      481 ggtcggctca gtgggcgacc ggacacccgc cggcggtgaa atcggccggc atgtgctgaa
      541 gtgcgagaac gagcgaggac tcgccgcggg gaaccttcgc gcccgcgggg acggtgatgt
      601 cgggcatgcg ggagagcagt tcctggaaca ccagcttgac ctccattcgg gcgaggttgg
      661 ccccgaggca atagtgcggg ccgatgccga aggccatgtg cggattctcc gggcgctgga
      721 agtccaagac gtccgggttg gaaatggcgc gctcgtcgcg gttcgccgcc gcgtacagca
      781 tcaggacgcg gtcgcccttc ttgaggtcgg tgttgcgata ggtgtggtcc tgttcgaccg
      841 tgcgaacgaa gccgagcacc ggcgtcacgt agcggatgag ctcctcgatc gccgggtcca
      901 tgaactcgtc gttgaaaagg ttgtcgatca tcaactgctt ctgttcgggg tactcgctga
      961 gcgccagcag gccgccggtg atggcgttgc gggtggtctc gttgccggcg accatcaaca
     1021 cggcgaggaa cgcgaacagc tcgtcgtcgt tgagggccga cttttcgagc atcgcctggc
     1081 gggtcgcgtc gtcgaccccc tgatagacct tctcgtgttc acgctcgagg cctccctcgt
     1141 cgtaggcctt ggtgaggatc gagatgaggt cgtctttggg gtcctcgcgg cgctcggcga
     1201 tgaggcccat gcacaacacg gcgaactcgc cgaaggcctc ggccgccgcg agcagcaccg
     1261 ggtcgtcgcc gtcgacgtgg ccgtcgcccg ccatcatctg gtcgctccag cggtacatgt
     1321 ccatgcgctg ggccggatcg agtcccatca gttcgcagat gatgatgagg ggcacgtgga
     1381 tggcgaactg ctcgacgaaa tcgatgtggc cggagtcgat gacgtcatcg atgagctggc
     1441 cggcgagttc gcggatgtgg ggcatcatcg cccgcacccg gcggggcgtg aagcccttgt
     1501 tgatgaggcg gcgggtggcg acgtggtcgg ggccgtccat ggtgatgatc gacatgtcgc
     1561 cggcgatgat ggggcgaacg ccgaaccgcg agacgtacag ctcgttgtcg cggctgatgt
     1621 ggaagacgtc ctcgtggcgg ggggcgatgt agaggtcgtt cttggcgtcg tagtggaggt
     1681 gatcgaggtc tcgcagcgct gcgtaggtgt cccacggatc gtcgaaggtc gacgggtcgg
     1741 tgaagtcgac gatgtcgagg tcgaccggct gacggtcgcg ggtgggcgag aacggcgtga
     1801 acacaggcga ggtcatgcgg cagtctagct aattgaacgt tcgtttggga agagtct
//
LOCUS       DAOOMA010000573         1853 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_79900_length_1853_cov_3.286429, whole genome shotgun sequence.
ACCESSION   DAOOMA010000573 DAOOMA010000000
VERSION     DAOOMA010000573.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 1853)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1853)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1853
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_79900_length_1853_cov_3.286429"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..789
                     /gene="sucD"
                     /locus_tag="PLP95_12680"
     CDS             <1..789
                     /gene="sucD"
                     /locus_tag="PLP95_12680"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012224005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HPB46705.1"
                     /translation="GTSPSKAGTDVDGIPIFATVAEAVEATGATASCIFIPAPGVKNA
                     VIEAAEAGIKFIVVITEGVPAHDEAWFYNKLQRDFPDVQLLGPNCPGIISPGKANIGI
                     TAGHIAKPAVAGEKNVGIVSRSGTLTYQALYELQQQGIGVTTCVGIGGDPVPGTTFID
                     CLKAFEADPETHAIMMIGEIGGSAEEEAAEFIKTSVTKPVSSYIAGVTAPAGKKMGHA
                     GAIVSGNAGTAQGKMDALRAAGVKVGLNPTEAGELMVEIIKGLG"
     gene            1373..>1853
                     /locus_tag="PLP95_12685"
     CDS             1373..>1853
                     /locus_tag="PLP95_12685"
                     /EC_number="1.1.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020379005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HPB46706.1"
                     /translation="MSSIIYTYTDEAPALATHSFLPIIQAFAKVAGVRVETRDISLAG
                     RIIANFPEGLSDTQRIGDALAELGDIVTHAEANVIKLPNVSASVPQLKAAIAELRAKG
                     YDVPEYPDDPADDNEVSIRERYAKVLGSAVNPVLREGNSDRRAAASVKNYAKKHPHRM
                     "
BASE COUNT          318 a          605 c          616 g          314 t
ORIGIN      
        1 ggcaccagcc cttccaaggc cggcaccgac gtcgacggca tcccgatctt cgccaccgtc
       61 gccgaagctg tcgaggccac cggcgccacc gcgtcgtgca tcttcatccc ggctccgggc
      121 gtgaagaacg ccgtgatcga ggccgccgag gcgggcatca agttcatcgt ggtcatcacc
      181 gaaggcgtgc cggcccacga cgaggcctgg ttctacaaca agttgcagcg cgacttcccc
      241 gacgtgcagt tgctgggccc gaactgcccc ggcatcatca gccccggcaa ggcgaacatc
      301 ggcatcaccg ccggtcacat cgccaagcct gcggtggcgg gtgagaagaa cgtcggcatc
      361 gtgagccgct cgggcaccct gacctatcag gcgctctacg aactgcagca gcagggcatc
      421 ggcgtgacca cctgtgtcgg catcggcggc gacccggttc ccggcacgac cttcatcgat
      481 tgcctcaagg cgttcgaggc cgaccccgaa acccacgcca tcatgatgat cggcgagatc
      541 ggtggttcgg cggaggaaga ggcggcggag ttcatcaaga ccagcgtcac caagccggtg
      601 tcgtcctaca tcgccggtgt gaccgcgccg gccggcaaga agatgggcca cgccggcgcg
      661 atcgtgtcgg gcaacgcggg cacggctcag ggcaagatgg acgccctgcg cgctgccggt
      721 gtgaaggtgg gtctgaaccc gaccgaggcc ggcgagttga tggtggagat catcaagggc
      781 ctcggctgag ccaatggcct cggctggtgc cgaccctgct ggccccgccg ctggttacag
      841 gcctcgaaac ccgccgctcc tggtgcatcc ttgcccggga cgcggcgggt tcggcgtttt
      901 cggggcggct aagggaagcg acgctccgcc agcccgaaca agatccgtgc gacttcccgt
      961 tgtaactcag tgagcccgct catgatgccg cttgtttgat cggctcgtga gcgtgttgcg
     1021 tgacgaggcc attccaagcg gctcgtagtt cgcgtggaag cacgagatcg gtccatgctt
     1081 cacagagcaa gacgccgtcg atggtttgtt cgatgtcggc aggtgttcct tcgcggagga
     1141 ggatttcgta gattcgaagt cgccctgctt caacggcact ggagacaaag cccgccaata
     1201 caaggcccgt tgccgatgtg gcttcgccgt gtttgccctt gtgagccggc cacaggaggt
     1261 cggcgcccct gcccgcccct gcccgcaggg gttggtggtg tttgtcgggt cgatctcgcg
     1321 actcgacggc ggtgttcgaa agcgggtcgg cgtcgggcgg tagccttgcg gcgtgagctc
     1381 gatcatctac acctacaccg acgaggcgcc ggcgcttgcg acgcactcat tcctgccgat
     1441 cattcaggcg ttcgcgaagg tcgcgggtgt ccgggtcgag acccgcgaca tctcgttggc
     1501 ggggcgcatc atcgccaatt tccccgaggg cctgagcgac acccaacgca tcggcgacgc
     1561 gctcgccgaa ctcggcgaca tcgtcaccca cgccgaggcg aatgtcatca agttgcccaa
     1621 cgtttcggcc tcggtgcccc agctcaaggc ggccatcgcc gagttgcggg cgaaggggta
     1681 cgacgtgccc gagtaccccg atgatccggc cgacgacaac gaggtgtcga tccgcgagcg
     1741 gtacgcgaag gtgctcggct cggccgtgaa cccggtgctg cgcgagggca actccgatcg
     1801 tcgtgctgcg gcatcggtga agaactacgc gaagaagcat ccgcatcgca tgg
//
LOCUS       DAOOMA010000574         1848 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_80215_length_1848_cov_3.383714, whole genome shotgun sequence.
ACCESSION   DAOOMA010000574 DAOOMA010000000
VERSION     DAOOMA010000574.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 1848)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1848)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1848
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_80215_length_1848_cov_3.383714"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..625)
                     /locus_tag="PLP95_12690"
     CDS             complement(<1..625)
                     /locus_tag="PLP95_12690"
                     /inference="COORDINATES: protein motif:HMM:NF013866.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HPB46707.1"
                     /translation="MTGSTETEFANGSPLEAYSRSSFTHEGLTRDIYRRGSGPAVIVI
                     AEMPGITPKVVAFADRVVDLGCTVVMPALFGEPGLDPNAMAVPAAALHMAATLTRACV
                     AKEFTVWSLGETSPIVGWLRALAAHEHERCGGPGVGAVGMCFTGGYALSMAVDDRLVA
                     PVLSQPSMPIALGRRHKAALDISPADLDRVKERARRGDLEVLGLRFCG"
     gene            716..1780
                     /locus_tag="PLP95_12695"
     CDS             716..1780
                     /locus_tag="PLP95_12695"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: protein motif:HMM:NF002872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent 6-phosphofructokinase"
                     /protein_id="HPB46708.1"
                     /translation="MSAPVRRLAINTGGGDAPGLNATIRGAVLGAIDQGWDVIGIRDG
                     YEGLFHDDGVVELHRDVVRDVTNVGGTILGTTNRGNPFETGRGEAVVQRLRELGVDIM
                     VAIGGDGSLTIGHKLSELGMPVIGVPKTIDNDLEHTDQTPGFDSAVSFATECIDRLVS
                     TTTAHDRIMVIEVMGRTAGWIALYSGIAGAAHAILIPEIPYDIEVVAQRVRQRAERGQ
                     DYSIVVVAEGAKPIGGSATTLQGTTRLGGVSQLVAEQLEELTGKEARWSNLGHLLRGG
                     SPTADDRLLGLRYGSEAIRAIVEGHRNVMIALRDGNAVTVPLSEVADRARLIPDDDGV
                     LRTARAIGICLGEPGRADAC"
BASE COUNT          325 a          653 c          601 g          269 t
ORIGIN      
        1 cgccacagaa ccgaaggccc aacacctcca ggtcgccccg cctcgcacgc tccttcaccc
       61 ggtcgagatc ggctggggag atgtcgaggg ctgccttgtg tctgcggccc aacgcgatcg
      121 gcatcgacgg ctgggacaac accggtgcga ccagccggtc atcgaccgcc atcgacagcg
      181 cgtacccacc ggtgaagcac atgccgaccg cgccgacccc cgggccgccg caccgctcgt
      241 gttcgtgcgc cgcgagcgct cgaagccacc cgacgatcgg gctggtctcg ccgagggacc
      301 agacggtgaa ctccttcgcc acgcacgccc gggtcagggt cgcggccata tggagcgcgg
      361 ccgccggaac ggccatggcg ttggggtcga ggccgggctc gccgaacagc gccggcatca
      421 ccacggtgca acccaggtcg acgactcgat ccgcgaaggc caccaccttc ggggtgatgc
      481 ccggcatctc ggcgatcacg atcaccgccg gaccgctgcc gcgccggtag atgtcgcggg
      541 tgagcccctc gtgagtgaac gacgagcgcg aataggcctc gaggggtgaa ccgttcgcga
      601 actctgtctc cgtggaccct gtcatgggtc agaagctacc ggcgcgggcg ctacggtcga
      661 gatatcgaat ccgtttctcc tcggccggac ctgccggccc cgaaagcgag gcgccatgtc
      721 cgcacccgta cggcgactgg ccatcaacac cggtggcggt gacgcccccg gtctgaacgc
      781 cacgattcga ggtgccgtgc tcggcgccat cgatcagggt tgggacgtca tcggaattcg
      841 cgacggctac gaggggctct tccacgacga cggagtcgtc gaactgcacc gcgacgtggt
      901 gcgcgacgtc accaacgtcg gaggcaccat cctcggcacc accaaccgcg gcaacccctt
      961 cgagacgggc cgcggcgaag cggtcgtgca gcgccttcga gaactcggcg tcgacatcat
     1021 ggtggcgatc ggcggcgacg ggtctctcac gatcggccac aagctctccg agttgggcat
     1081 gccggtcatc ggggtcccca agacgatcga caacgacctc gaacacaccg atcagacccc
     1141 gggcttcgac agcgctgtca gctttgccac cgaatgcatc gaccgactcg tctccacgac
     1201 gaccgcgcat gaccggatca tggtgatcga ggtcatggga cgcaccgcgg gatggatcgc
     1261 cctgtactcc ggcatcgccg gcgccgcaca cgccatcttg attccggaga tcccctacga
     1321 catcgaggtc gtggcgcaac gggtccgcca gcgcgccgag cgcgggcagg actactcgat
     1381 cgtcgtcgtc gccgaaggtg ccaagccgat cggtggaagc gctaccacgc tgcagggaac
     1441 gactcgactc ggcggcgtgt cgcaactcgt cgccgaacaa ctcgaggaac tgaccggcaa
     1501 ggaggcgcgc tggtcgaacc tcgggcacct gcttcggggc ggttccccga cggctgatga
     1561 ccgcctcctc ggcctgcgct acggctccga ggcgatccgg gcgatcgtcg agggtcaccg
     1621 caacgtgatg atcgccctgc gcgacggcaa cgccgtcacc gtgccgctca gcgaggtggc
     1681 cgatcgcgcc cgcctcatcc ccgacgacga cggagtgttg cgcacggctc gggcgatcgg
     1741 aatctgcctc ggcgagccgg ggcgggccga cgcctgttga cccacccaca caagacgttc
     1801 tgtaggtaat tgcggttgcc atggcaaccg caattaccta cagaacgt
//
LOCUS       DAOOMA010000575         1830 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_81406_length_1830_cov_3.232676, whole genome shotgun sequence.
ACCESSION   DAOOMA010000575 DAOOMA010000000
VERSION     DAOOMA010000575.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 1830)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1830)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1830
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_81406_length_1830_cov_3.232676"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(128..1546)
                     /locus_tag="PLP95_12700"
     CDS             complement(128..1546)
                     /locus_tag="PLP95_12700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HPB46709.1"
                     /translation="MTNLESTPSTTAATPSFTELVDGVRATFNSGRTRPMTWRRRQLE
                     GMLQMLKVHEQDFVDAIVSDLGRPVLEAFSADIGHARLQIKHVLKHFEQWARTEKVSP
                     GLLSQPGTAEIIKEPLGVALVIAPWNYPVQLLLEPMAAALAAGNAVVAKPSELAPASA
                     AVLARLIPQYLDDDAVVVVEGGVPETTALLEEPFDHIFFTGSTNVGRVVMTAAAKHLT
                     PVTLELGGKSPTIVAADADLAIAAKRIVWAKLMNAGQTCIAPDYVLVDETVKDRFVDL
                     VVDAIRTFVGADPKQSSDLGRIISPRHLDRLVGLVSGGGGTTVVGGDHDQATKYLAPT
                     VIVDPDLESNLMTEEIFGPVLPIVSVVSVDEAIEFVNARPKPLALYVFTQSSTTADLL
                     LRRTSSGGACVNHAVVHILPDGLPLGGVGPSGMGAYHGRTGFDAFSHHKSVVRKKTRP
                     DMPLLYAPYGGWKERIVRFVFR"
BASE COUNT          328 a          611 c          592 g          299 t
ORIGIN      
        1 acctcatggg cactctcggt ctgggtcgaa gcccttctcg gccaacccta aaaacctcgg
       61 caaacacggc cacagagccg cccacgcgcc ggccggccga ccgaccgacc ggccgatcag
      121 tgcgggctca gcggaacacg aacctcacga tgcgctcctt ccaacccccg tacggggcgt
      181 agagcaatgg catgtccgga cgggtcttct ttcgaacgac cgacttgtgg tggctgaacg
      241 cgtcgaagcc ggtgcggccg tgatacgccc ccatccccga tgggcccact ccgccgagcg
      301 gcaacccgtc ggggaggatg tgcacgaccg catggttcac acaagcacct ccggagctcg
      361 tgcgtcgaag cagcaggtcg gccgtggtcg acgactgggt gaacacatac agcgccagcg
      421 gcttcggtcg agcgttgacg aactcgatcg cctcgtcgac cgacaccacc gacacgatcg
      481 gcaggaccgg cccgaagatc tcctcggtca tcaggttcga ctcgaggtcc ggatcgacga
      541 tcacggttgg cgccaggtat ttcgtcgcct ggtcatgatc cccacccacc accgtggtcc
      601 cgccaccgcc ggacaccaat ccgacgagcc ggtcgaggtg ccgcggcgag atgatgcgac
      661 cgagatcgct gctctgttta ggatcggcac cgacgaaggt gcgaatagcg tcgaccacca
      721 gatcgacgaa gcgatccttc accgtctcat cgaccagcac gtagtcgggg gcgatgcagg
      781 tctggccggc gttcatgagc ttcgcccaca cgatgcgctt cgcagcgatg gcgagatccg
      841 cgtcggccgc gacgatcgtc gggctctttc cgccgagttc gagggtgacc ggcgtgaggt
      901 gtttggccgc cgcggtcatc accacgcggc cgacattggt ggatccggtg aagaagatgt
      961 gatcgaaggg ttcctcgagc aatgccgtgg tctcgggcac tcccccttcc accaccacga
     1021 cggcgtcatc gtcgaggtac tgcgggatca ggcgggccaa caccgcggcg ctcgccgggg
     1081 ccagttcgga cggcttggca accaccgcgt tgcccgcagc gagcgcggcg gccatcggtt
     1141 caagcagcag ctgaaccgga tagttccacg gggcgatcac cagcgccacg ccgagcggct
     1201 ctttgatgat ctcagcggtt cccggctggc tgagtaggcc ggggctcacc ttttcggtgc
     1261 gggcccactg ctcgaagtgc ttcagcacgt gtttgatctg caggcgggcg tgtccgatgt
     1321 cagccgagaa cgcctcaagc acgggccggc caagatccga gacgatggcg tcgacgaagt
     1381 cctgctcgtg gaccttgagc atctgaagca ttccctcgag ctgacgacgc cgccacgtca
     1441 tcggccgggt gcggccgctg ttgaacgtcg cccgcacccc gtccaccagt tccgtgaacg
     1501 acggcgtggc cgcagtggtc gatggggtgg attcgagatt ggtcatggat gcccctgcgc
     1561 taggtgcccg aaagcgactg tccggcttga gggcaacgtt actcctcggc gcacctggtc
     1621 ctagaaacct cgggtccccg cagccgagac cgcggggacc cgagatgggc aattcggttt
     1681 gagcgagaca gcactcggca gcatccggcc gcgtccaccc gtgctcagca gcgtcaagca
     1741 gcgtcaaacg gggtcgagtg cgcgctggcg aagctcaggg gttgagccga tcgaggatca
     1801 gatcactctg cgccgcacgc agcgccgagc
//
LOCUS       DAOOMA010000576         1826 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_81684_length_1826_cov_4.649915, whole genome shotgun sequence.
ACCESSION   DAOOMA010000576 DAOOMA010000000
VERSION     DAOOMA010000576.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 1826)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1826)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1826
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_81684_length_1826_cov_4.649915"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..316)
                     /locus_tag="PLP95_12705"
     CDS             complement(<1..316)
                     /locus_tag="PLP95_12705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HPB46710.1"
                     /translation="MSDTSIPNVLAARYASTEMVEVWSPRRKVILERQLWIAVMRAQA
                     ELGIDIPAEAIAAYESVIDQVDLTSIAERERVTRHDVKARIDEFCALAGHEHIHKGMT
                     SRD"
     gene            complement(347..901)
                     /gene="purE"
                     /locus_tag="PLP95_12710"
     CDS             complement(347..901)
                     /gene="purE"
                     /locus_tag="PLP95_12710"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: protein motif:HMM:NF012934.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HPB46711.1"
                     /translation="MYAATSRPLQPKRRGDGADRNPNGGRPPPLLHGDDVKVAILMGS
                     ASDTDKMKPASETLAKFGIEADVRVLSAHRNHSQVSDLVSSARDNGYVAFICGAGMAA
                     HLAGAVAAQTTLPVVGVPLSGGALNGVDALYSTVQMPKGIPVATVAVDGSMNAALLVV
                     QMLAISDADLAAKLADHRAEMAGA"
     gene            complement(855..>1826)
                     /gene="purD"
                     /locus_tag="PLP95_12715"
     CDS             complement(855..>1826)
                     /gene="purD"
                     /locus_tag="PLP95_12715"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HPB46712.1"
                     /translation="KAYMKELLVEAGVPTAAHGTFTDEAAALEFLDQMTSPYVIKTDG
                     LAGGKGVLVTESLDEARQAVRRYLSGAAFGAAGRTLIVEEGLSGPEISVFAICDGTKA
                     VLLSPAQDFKRINDGDEGPNTGGMGAYTPVPGVDDAVLADVLNRFINPTLETLRSRGI
                     DYRGVLFAGLMLTADGPRMLEYNVRFGDPECQVVTPRLRNDLAQILAEAAAGDLRTTP
                     DYGEDALVNVVCAASGYPQNPRTGDVITGIEAAAAREGVTVYCAGVARNDEGDLVTAG
                     GRVLNVVGRGATIAEARERAFAAIDDIQFDGMHVRRDIAAAAAEASW"
BASE COUNT          313 a          613 c          576 g          324 t
ORIGIN      
        1 ggtcgcgact cgtcatcccc ttgtggatgt gttcgtggcc ggccagtgca cagaactcgt
       61 cgatgcgcgc cttcacgtcg tggcgggtga cccgttcacg ctccgcaatg gatgtcagat
      121 cgacctgatc gatcaccgac tcgtacgcgg cgatcgcttc ggcggggatg tcgatgccga
      181 gttccgcctg ggcacgcatg actgcgatcc acaactgccg ctcgaggatc accttgcggc
      241 gcggcgacca cacctcgacc atctcggtcg atgcgtagcg ggctgcgagt acgttgggaa
      301 tcgaggtgtc gctcacggga tcctcatcgg gtggggtgct gggagttcaa gcgccggcca
      361 tctcggcgcg gtgatcggcg agtttggcag cgagatccgc gtcactgatc gccaacatct
      421 gcacgacgag cagggctgca ttcatcgacc cgtcgaccgc gaccgtcgca accggaatgc
      481 ccttgggcat ctgcacggtc gaatacaggg cgtcgactcc gttgagcgca ccacccgaca
      541 gcggcacacc gacgaccggc agggtggtct gggcggccac ggctccggcg agatgcgcag
      601 ccattccggc gccgcagatg aacgcgacgt aaccgttgtc gcgggcgctc gataccagat
      661 cggagacctg cgagtggttg cggtgtgccg acagcacccg gacatctgcc tcgataccga
      721 atttggcgag cgtctcgctc gccggcttca tcttgtcggt gtccgatgcg gaccccatca
      781 gaattgcaac cttcacatca tctccatgca agaggggcgg gggtctccca ccgttcggat
      841 ttcgatctgc gccatcacca cgacgcttcg gctgcagcgg ccgcgatgtc gcggcgtaca
      901 tgcatcccgt cgaattggat gtcgtcgatc gcggcaaagg cccgttcgcg ggcctcagcg
      961 atcgtggcgc cgcgccccac gacgttcagc actctgccac ccgcggtgac gaggtcgccc
     1021 tcgtcgtttc gagcgacccc agcgcagtac accgtgactc cctcgcgggc cgcagcggcc
     1081 tcgatgccgg tgatcacatc gccggtacgg gggttctggg gatatcccga cgcggcgcac
     1141 acgacgttca ccaaggcatc ctcgccatag tcgggagtgg tgcgcagatc gccggcggcc
     1201 gcctcggcca ggatctgagc gaggtcattg cgcaatcgag gggttacgac ctgacactcc
     1261 gggtcaccga atcgcacgtt gtactcgagc atccggggac cgtcggcggt cagcatcaat
     1321 ccggcgaaca gcacgccgcg gtagtcgatg ccccgcgacc ggagcgtctc caacgtcggg
     1381 ttgatgaagc gattcaacac gtccgccaac acggcgtcat cgacaccggg gaccggggtg
     1441 taggccccca ttccaccggt gttcggcccc tcgtcgccat cgttgatccg cttgaagtcc
     1501 tgggccggcg acaacaggac ggccttggtg ccgtcgcaga tggcgaacac cgagatctcc
     1561 ggcccgctga gcccctcctc gacgatgagc gtgcgacccg cagctccgaa cgctgcgccg
     1621 gaaagatatc gacgaaccgc ttgtcgggcc tcgtcgagcg attcggtgac gagcacaccc
     1681 tttccaccgg ccagtccatc ggtcttgatg acgtacggcg aggtcatctg atcgaggaac
     1741 tccaatgccg cggcctcgtc ggtgaaggtc ccatgcgccg ccgtcggaac gccagcctca
     1801 accaacagtt ccttcatgta ggcctt
//
LOCUS       DAOOMA010000577         1820 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_82131_length_1820_cov_3.366572, whole genome shotgun sequence.
ACCESSION   DAOOMA010000577 DAOOMA010000000
VERSION     DAOOMA010000577.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 1820)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1820)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1820
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_82131_length_1820_cov_3.366572"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(133..1368)
                     /locus_tag="PLP95_12720"
     CDS             complement(133..1368)
                     /locus_tag="PLP95_12720"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HPB46713.1"
                     /translation="MPRAFRGPHTSSTALDIGVYGARGVPSTYSGYETFLTLLLPELA
                     KRGDRVTMYCRSGEDFTNDDWQGVHRKVLPTIPGKNFSTLSHGLVAGVAARFARHDVV
                     LVVNVANAAYCALSRYTRQPVVLNVDGQEWLRGKWGNSAKTIFLRSAQISRHCASALI
                     ADGAAMADIYRDEFASDTTVIPYCVTNGDWTKNPATVQNLGLEPYRYLLIAGRHNPEN
                     NIDRIVRDYAAGSHPLPLVVLGTANYDSPVTTAIAELAERDERIRMLGHVGDRNAFFD
                     LVHHAKVYLHGHSVGGTNPSLVEAMGTGSRIAAFDTAFSREVLGDNAEYFNLDETAGP
                     NAGLNSLDRAVTTILDDNPTHDLHVRDQTAARAGDLYNVDDVVGAYRDLLAAAANSRR
                     PVSLRTRWAAGTRANAASR"
     gene            1626..>1820
                     /locus_tag="PLP95_12725"
     CDS             1626..>1820
                     /locus_tag="PLP95_12725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB46714.1"
                     /translation="MSTSLPSDQPRASIRSRTLGRAALAVFAVFGVVAGRLALGNVGA
                     DEIPPPPAQSPSSEPTPRDPG"
BASE COUNT          262 a          542 c          677 g          339 t
ORIGIN      
        1 cgaggcgcga caacgccagc atctgcagtg cgaggctcga agcggcgacc accccctggc
       61 tggcgatcgc ggcgccggca ccgccgacga cgttgcgccg ctgtgtgatc ttggcgacga
      121 acccggtctg catcagcgtg aggcagcgtt cgctctggtg ccggccgccc agcgggtccg
      181 cagggaaacg ggtcggcggc tgttggccgc ggcggcgagc aggtctcggt acgcgccgac
      241 cacgtcgtcg acgttgtaca ggtcgccggc gcgcgctgcg gtctggtcgc gcacgtgcag
      301 gtcgtgggtg gggttgtcgt cgaggatcgt ggtcacggcg cggtcgaggc tgttcagtcc
      361 ggcgttcggt ccggcggttt cgtcgaggtt gaaatactcg gcgttgtcgc cgagcacctc
      421 gcgggagaac gcggtgtcga acgcggcgat gcgtgagccg gttcccatcg cctcgacgag
      481 gctggggttg gtgccgccga cggagtggcc gtgcaggtac accttggcgt ggtgcacgag
      541 atcgaagaac gcgttgcgat cgccgacgtg gccgagcatg cggatgcgct cgtctcgttc
      601 ggcgagctcg gcgatggctg tggtcaccgg cgagtcgtag ttcgcggtgc cgagcaccac
      661 taggggcagc gggtgggagc cggccgcgta gtcgcgcacg atgcggtcga tgttgttttc
      721 ggggttgtgg cggccggcga tcaacaggta ccggtagggc tcgaggccga ggttctggac
      781 cgtcgcgggg tttttcgtcc agtcgccgtt ggtgacgcaa taggggatga cggtggtgtc
      841 ggaggcgaat tcgtcgcggt agatgtcggc catggcggcg ccgtcggcga tcagggcgct
      901 ggcgcagtgg cgcgagatct gggcggacct caagaagatg gtcttggcgc tgttgcccca
      961 cttgccccgc agccactcct gtccgtcgac gttgagcacg acgggctgtc gggtgtagcg
     1021 cgacagtgcg cagtaggcgg cgtttgccac gttgacgacc aacacgacgt cgtggcgggc
     1081 gaaccgggcg gcgactccgg cgacgaggcc gtggctcagc gtcgagaagt tcttgccggg
     1141 aatcgtgggg agaaccttgc ggtgtacccc ttgccagtcg tcgttggtga agtcctcgcc
     1201 gcttcggcaa tacatcgtga cccggtcgcc gcgtttggcg agttcgggga gcagcagggt
     1261 gaggaaggtc tcgtaaccgg aataggtgga gggcacgccc cgggcgccgt agacgccgat
     1321 gtcgagtgcc gtgctggagg tgtgggggcc tcggaaggct cgtggcattc gctcaggcta
     1381 tcggcgggtt cgcgaaggct tgcacggacc gggtggggta tttcaccctt cgcgcgggtc
     1441 gcagcgctca gcgtcgtgaa tctcgccgaa ttcgggtgtc taccgggcac tttcgcccat
     1501 gccgcctgag gcggatttcg gccgcaacgg tactaaattc ttaattgaaa cacgcgcatt
     1561 gtcgattcaa tgtgttaccg gcgaattcat ctgctgtagc gaccagcgtc gccgaaggag
     1621 aaccagtgag cacatccctt ccatccgatc agccgcgggc gtcgatacgt tcccgcacgc
     1681 tcggacgtgc agcgcttgcc gtgttcgccg tgttcggtgt cgtcgccggc aggctggcac
     1741 tcggcaacgt cggcgccgac gagatcccac ctccgccggc ccagtctccg agttccgagc
     1801 ccactccgcg cgatccgggc
//
LOCUS       DAOOMA010000578         1820 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_82140_length_1820_cov_3.018130, whole genome shotgun sequence.
ACCESSION   DAOOMA010000578 DAOOMA010000000
VERSION     DAOOMA010000578.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 1820)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1820)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1820
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_82140_length_1820_cov_3.018130"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(87..389)
                     /locus_tag="PLP95_12730"
     CDS             complement(87..389)
                     /locus_tag="PLP95_12730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB46715.1"
                     /translation="MEQSGTDGSLDDFLQRVDDVLDRAFPGESASVEEVEAVAGAGAA
                     AGVEASVVSRVSDDRRFGTFTSPPPSPLQPASPLPPPSQPATPHALHQIDPSELLA"
     gene            495..1010
                     /locus_tag="PLP95_12735"
     CDS             495..1010
                     /locus_tag="PLP95_12735"
                     /EC_number="1.-.-.-"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: protein motif:HMM:NF013412.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine-phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HPB46716.1"
                     /translation="MPEAQRPAMAEYGVPDDLDGTLPWDWARQRLVANRNYWVVTVDA
                     DHRPHSTPVWGVWVDDDTFWFSCAHTALKARNLRANPHVAVTTADTVEFVSVEGVAVE
                     LTPPAEVARAWALKYRDADNGTDASDADDTTEMEAFFSGNAAFQVRPTKVIGMIERPD
                     EFAQRATRWVF"
     gene            complement(1124..1303)
                     /locus_tag="PLP95_12740"
     CDS             complement(1124..1303)
                     /locus_tag="PLP95_12740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB46717.1"
                     /translation="MKAREGVVVGIVGVMIGAAIDASWRADERASCRDRLLLATGVEA
                     TMLPRSSPLRDRRLD"
     gene            complement(1300..1548)
                     /locus_tag="PLP95_12745"
     CDS             complement(1300..1548)
                     /locus_tag="PLP95_12745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB46718.1"
                     /translation="MQPVFLDHRLPRLAHGSLLLIAAVALVMGAVGWVALGAHPLSGP
                     ILVNLNSHRGIHITDILAAVPLGVALALSIEAWRVIRP"
BASE COUNT          341 a          620 c          591 g          268 t
ORIGIN      
        1 acggcatgtt cggagcaact gccgctctac acccgcaatc cggctgattt cgcggccctg
       61 gatcgactcg tcgaagtcgt cgccgtctag gcgagcagtt ccgagggatc gatctgatgg
      121 agcgcgtgcg gggtggcggg ctgtgatggt ggagggagtg gtgatgctgg ctggagtggt
      181 gatggtggcg ggctggtgaa ggtaccgaag cgacggtcgt cactcacgcg agacaccacc
      241 gacgcctcga ccccggccgc cgccccagcc ccagccaccg cctcgacctc ctccactgag
      301 gccgactcgc cggggaacgc ccgatcgagc acgtcatcca cacgctgaag gaagtcatcg
      361 agcgacccat cggtacctga ttgctccacg tcactaccct cacgtcgatc tgctcgtcga
      421 cctgcacgtc gagcagaggc tacgggagac gaagcttccc cacccacgtg ccgtcagcgg
      481 gtacggtgcc gaccatgccc gaagcacaac gcccagcgat ggccgaatac ggcgtccccg
      541 acgacctcga tggcacgctc ccgtgggact gggcccggca acggctggtc gccaatcgca
      601 actactgggt cgtcaccgtc gatgccgacc accgacccca ctccacgccg gtgtggggcg
      661 tgtgggtcga cgacgacacg ttctggttct cgtgcgccca caccgcgctg aaggcacgaa
      721 acctgcgggc caacccacac gttgcggtca ccaccgccga cacggtcgag ttcgtgtcgg
      781 tcgaaggggt cgcagtcgaa ctcacgccac ccgccgaggt cgcccgggcc tgggcgctca
      841 agtaccgcga cgccgacaac ggcactgacg ccagcgacgc cgatgacacg accgagatgg
      901 aggcgttctt ctccggcaac gcggccttcc aggtgcgccc gacgaaggtg atcgggatga
      961 ttgaacggcc cgacgagttc gcccagcggg cgacccgatg ggtcttctag cgcggcgcgg
     1021 ccgaagcggc cacaaccacc gcacggggtc cgtcgatgag acgccggcaa tgcaggcaac
     1081 atcaacgggt gaacgcagcg tcctggccgc gaacgccggt atctcagtcg agtcgacggt
     1141 cgcgtagcgg cgaggaacgg ggcaacatcg tcgcctcgac tcccgttgcg agcaggagcc
     1201 gatcgcggca actcgcccgc tcatccgccc gccagctcgc gtcgatggcc gcgccgatca
     1261 tcactcccac gatcccgacc acgacacctt ctcgcgcctt catggccgga tcacccgcca
     1321 cgcttcgata ctgagtgcaa gcgcaacgcc cagcggcacc gccgccaaga tgtcggtgat
     1381 gtggatgcct cggtgactgt ttagattcac gagaatcggg cccgacagcg ggtgtgcacc
     1441 gagagcgacc cacccgacag cacccatcac aagcgccacg gcggcgatga ggagcagcga
     1501 tccgtgcgcc agacggggta agcggtgatc aagaaagacc ggttgcatgg atcacatgat
     1561 ggggaccggc cggttgtcgg ctgactagac caaggtgagg ggacggtaaa acctcgcccg
     1621 atggtgtcgg caccaacgcc ccgctccggc acactgaggg cgtggaacag gtcctggtgg
     1681 ttgaagatga cgagggaatt cgctcgggcc tcgtgcgtgc gttgggcaac gcgggctacg
     1741 agtgcatcgc cgctcccgac gcggccaccg tcaccttcgt ggaatcggct cccaggtata
     1801 catacaccga cggcgcggcg
//
LOCUS       DAOOMA010000579         1818 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_82294_length_1818_cov_2.752127, whole genome shotgun sequence.
ACCESSION   DAOOMA010000579 DAOOMA010000000
VERSION     DAOOMA010000579.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 1818)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1818)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1818
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_82294_length_1818_cov_2.752127"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..953)
                     /locus_tag="PLP95_12750"
     CDS             complement(<1..953)
                     /locus_tag="PLP95_12750"
                     /inference="COORDINATES: protein motif:HMM:NF024571.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HPB46719.1"
                     /translation="MSDQYVRRFLDDELSEVIRVHPAVLVVGPRASGKTTTARRYAKS
                     VVRLDRPAEAAAFVADADAALASFDEPVLLDEWQAVPGVLGAVKRAVDDDSRPGRFIL
                     TGSIRADLVAETWPGTGRLIRLDMWGLSEREIVGSTGERGPLAVLAAGDPMALGTPTA
                     PPDLVGYIELALRGGFPDTALRLDGSGRDRWLTSYLDQLLTRDAVDISGRDPVLLERY
                     FETLALNSAGIVADNTIFGAAAVDRRTANAYERLLSNLFVLDAVPAWLTNRLSRLVKT
                     PKRYITDASLIGAALRMDARSVMRDGDILGRVIDTFVAAQLR"
     gene            complement(1475..>1818)
                     /locus_tag="PLP95_12755"
     CDS             complement(1475..>1818)
                     /locus_tag="PLP95_12755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB46720.1"
                     /translation="PLTFVGILLAVLIASTSVFNVEISRTGLAVRSILGLPRFRVPIE
                     DIESVELIDIDPMGEFGGFGVRSRRGRRAVVLRRGDALEVTRRSGKRFAVTVEHPQDA
                     VAVLRALLDQR"
BASE COUNT          332 a          561 c          604 g          321 t
ORIGIN      
        1 cgaagttgtg cagcgacgaa ggtgtcgatc acacgaccca ggatgtcccc gtctctcatg
       61 acgctgcggg cgtccatgcg gagtgctgcg ccgatgagcg acgcatcggt gatgtagcgc
      121 ttcggcgtct tgacgagtcg cgagagtcga ttcgtgagcc acgcgggcac ggcatcgagg
      181 acgaacaagt tcgagagaag ccgctcgtaa gcgtttgccg tccgccgatc gacggcagcg
      241 gctccgaaga tcgtgttgtc agccacaatc cccgcggagt tgagcgccaa cgtctcgaag
      301 taccgctcca acaggacggg atctcgaccc gagatgtcga cagcgtcacg cgtcagtagc
      361 tgatcgaggt agctggtgag ccagcgatcg cgacctgatc catccagcct gagcgcagtg
      421 tcggggaatc cgccacgaag cgccagctcg atgtagccga caaggtccgg tggcgctgtt
      481 ggtgtgccga gcgccattgg atcaccggcg gccagtaccg cgagcggacc ccgttcgccg
      541 gtggagccta cgatttcgcg ctcggacagc ccccacatgt cgagacggat cagccgaccg
      601 gtcccgggcc atgtctcggc cacaaggtcg gcccgaatgc tgcccgtcag aatgaaccga
      661 ccaggccgac tgtcatcgtc cacggcacgc ttgactgcac cgagcacgcc aggaacggcc
      721 tgccactcgt cgaggagcac cggctcatca aaactggcga gggccgcatc ggcgtccgcg
      781 acgaacgcgg ccgcctcggc gggccggtcg agacgaacga cgctttttgc gtaccggcga
      841 gcggtcgtgg tctttccgga tgcacgtggt ccgacgacaa gtacagcggg gtgcacgcgg
      901 atgacctcgc tgagctcgtc atcgaggaat cgacgtacgt actgatcgga catgtcgcca
      961 ggataccgtc gcagaccagg cttcgatgct catctggacc accgcgcgat gtcgttctag
     1021 accactcacc gatgtcggtc tggaccgtcg gtctggaccg tcggtccggg tggtcggcgc
     1081 tgggtggtcg gcacgcgctc gccgctcgcc gccgctcggc gctgctcgtc cgcgaagttt
     1141 ctgccagaaa cgacatcacg tgtcactttt ggcagaaagt tcgcgaggag atcggtgacc
     1201 aaacgccgcc atgaacggcg tcggggcggg gtccgtagtc ggacgcccac cccgacgact
     1261 gctcgccaca gcgaatgggt gcatttggcg atgtgcccac atcggaagtg gtggcaatgt
     1321 gccccagcgg cacagcgcct cgttggaggt cacgggtgac atcgtgtcga agcgcccaag
     1381 cagcatttca agcatcgatg ccgcttgtca atgaattcca aagatccgag ggtttgtggg
     1441 ttgattctgt ttctgttcag ttcgccggca tggctcagcg ctggtcgagc agagctcgca
     1501 gtacggctac ggcgtcttga gggtgttcga cggtgacggc gaagcgtttg cccgatcggc
     1561 gggtgacctc gagggcgtcg ccgcggcgca acaccacggc tcgccggccg cgtcgtgaac
     1621 gcacgccgaa gccgccgaat tcgcccatcg ggtcgatgtc gatgagttcg accgattcga
     1681 tgtcctcgat cggcacgcgg aatctcggaa gtccgaggat cgagcgcacg gcgagtccgg
     1741 ttcggcttat ctcgacgttg aacacgctgg tcgacgcgat gagtaccgcg aggaggattc
     1801 cgacgaacgt gagcggtg
//
LOCUS       DAOOMA010000580         1811 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_82771_length_1811_cov_4.208998, whole genome shotgun sequence.
ACCESSION   DAOOMA010000580 DAOOMA010000000
VERSION     DAOOMA010000580.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 1811)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1811)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1811
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_82771_length_1811_cov_4.208998"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..747
                     /locus_tag="PLP95_12760"
     CDS             <1..747
                     /locus_tag="PLP95_12760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HPB46721.1"
                     /translation="NLGISHRRAIVAAGSAGLGLATARALAGEGVAVAICGRNPDRLN
                     AALASLGSGAVAIVADMSVPGDATRFAAEAQDRLGGTIDIVVANAGGPPPGTALSTDP
                     DAYRGALELNFISGVELIQAVVSPMRAQRWGRVIAVTSIGARQPIANLAASSSARAAM
                     TSFIKTLSLEIAADQVTANTIQPGLHATDRVLALHGGDPSGAAASVPVGRLGDAAEFG
                     AAAAFLCSEHAGFITGTSLLLDGGASTALI"
     gene            complement(809..>1811)
                     /locus_tag="PLP95_12765"
     CDS             complement(809..>1811)
                     /locus_tag="PLP95_12765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020378422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HPB46722.1"
                     /translation="SSSRRWRAASRAPALASGSCIILKPSPETPISVLRLAQLIEQAG
                     VPAGVVSVLPAGREVGEHLVTAPGIDKVSFTGSTAAGRRIGALCGERLRRVTLELGGK
                     SAAVVMPDADFVEVAPQLLPYGIMNNGQACVAQTRILVPRSRYDEAVEAFSNAVSALK
                     VGDPAEMDTDVGPLIAERQRDRVEGYIAKGIAEGARVAVGGGRPDSESGWFVEPTLFV
                     DVDNKMTIAQEEIFGPVLAMIAYEDIDDAIEIANDSDFGLSGSVWGPDVDAALDVARQ
                     IRTGTVSINGFAMEWSAPFGGFKDSGLGRELGPEGLEAYLEDKSIALPAGYEPTLTQ"
BASE COUNT          299 a          640 c          578 g          294 t
ORIGIN      
        1 aacctcggaa tttcacatcg gcgtgcaatc gttgcggcag gatctgccgg actcgggctc
       61 gcgaccgcac gggcgctcgc tggtgagggg gtcgccgtgg cgatctgtgg gcgaaacccc
      121 gaccgtctga acgccgcgct cgcctcgctc ggcagcgggg ccgtcgcgat cgtcgccgac
      181 atgtccgtgc caggggacgc gacccgtttc gcagcggagg cgcaggaccg acttggcgga
      241 accatcgata tcgtggtcgc caatgctggc gggccgcctc cgggcaccgc gttgagcacc
      301 gacccggacg cctaccgcgg cgcgctggaa ctcaacttca tctccggagt cgaactgatc
      361 caagcggtcg tctcgccgat gcgggcgcaa cgctggggtc gggtgatcgc cgtcacctcg
      421 atcggggcgc gtcaacccat cgcgaatctc gccgcgtcga gttcggcgcg cgcagcgatg
      481 acctcgttca tcaagacgct ctcgttggag atcgccgccg accaggtgac cgccaacacg
      541 atccagcccg gccttcacgc caccgatcgg gtcttggcgc ttcacggggg tgacccatcg
      601 ggtgcggccg cttcggtgcc ggtcgggcgt ctcggggacg ccgccgaatt cggagcggcc
      661 gccgcgttcc tgtgcagcga gcatgccggg ttcatcaccg gcacctcgct actactcgac
      721 ggcggcgcca gcaccgcgct catctgaccc gacagtgccg gctggcactg tcggccatgc
      781 agtgctgagt ggtggggtcg gtggaaacct actgagtcaa cgtcggctcg tagccagcgg
      841 gcaaggcgat cgacttgtcc tcgaggtacg cctcaaggcc ctccggccca agttcgcgac
      901 cgaggccgga gtccttgaac ccgccaaacg gcgccgacca ctccatggca aacccgttga
      961 tgctcaccgt gccggttcgg atctgacggg cgacgtcgag agcagcatcc acatcggggc
     1021 cccacaccga ccctgacagg ccgaaatcgc tgtcgttcgc gatttcgatc gcgtcgtcga
     1081 tgtcctcgta ggcgatcatc gccagaaccg gtccgaagat ctcctcctgc gcgatcgtca
     1141 tcttgttgtc gacgtccacg aacagcgtcg gctcgacgaa ccaaccggac tcactgtccg
     1201 ggcgtccgcc gccgaccgcg acgcgagcgc cctcggcgat gcccttggcg atgtagccct
     1261 cgacgcgatc gcgctgtcgc tcggcgatga gcggaccgac atcggtatcc atctccgctg
     1321 gatcgccgac cttcaacgca ctcaccgcgt tgctgaacgc ctccaccgcc tcgtcgtagc
     1381 ggctgcgcgg caccagaatg cgagtctggg caacgcacgc ctggccgttg ttcatgatcc
     1441 cgtaaggcag aagttgtggt gccacctcga cgaaatccgc atcgggcatc accacggccg
     1501 ccgacttgcc gccgagttcg agcgtcacac gccgcaaacg ttcgccgcac agcgcgccga
     1561 tgcgacgacc ggcggcggta gaaccggtga agctcacctt gtcgataccg ggcgcggtca
     1621 cgaggtgttc gcccacctcg cggcccgctg gcagcacact cacgacaccc gcgggaaccc
     1681 cggcctgctc gatgagttga gcaaggcgaa gcaccgagat cggagtctcg ggcgacggct
     1741 tcaaaatgat gcaacttccc gatgccagcg ccggggccct gctggccgca cgccaacgcc
     1801 tgctgctgga g
//
LOCUS       DAOOMA010000581         1811 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_82797_length_1811_cov_2.920843, whole genome shotgun sequence.
ACCESSION   DAOOMA010000581 DAOOMA010000000
VERSION     DAOOMA010000581.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 1811)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1811)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1811
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_82797_length_1811_cov_2.920843"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1071
                     /locus_tag="PLP95_12770"
     CDS             <1..1071
                     /locus_tag="PLP95_12770"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071111 - cyclic-guanylate-specific
                     phosphodiesterase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing phosphodiesterase"
                     /protein_id="HPB46723.1"
                     /translation="HQLREATIVAERIVAVLDEPMLIAGRWVSASASIGVAYGDRTMS
                     VSDLLRNADLAMYTAKKQGKSCYRVYEPEMHSAALERLEVEATLRGAAARGELFVEFQ
                     PIYDTRSGEMVEAEALVRWNHPERGLLGPGAFIPQAESTGVIEEVGEFVLRESLRWVR
                     EWGRDLGLDAVPAVNVNLSPRQLNDPRLPERVFAVLSELDLPASKLVLEITESAIMGD
                     PEQSRRILERISALGVRLAVDDFGTGYSSLAYLQQFPIAFLKIDGSFVQQMLVNEGAS
                     MVEAVTQLAHTLGLTPVAEGVESEAQLREVVRIGVDLAQGYHLSRPVSPDALFALLKS
                     QRRSDALSSLTLLNPPTPLDSL"
     gene            complement(1131..1502)
                     /gene="rnpB"
                     /locus_tag="PLP95_12775"
     ncRNA           complement(1131..1502)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="PLP95_12775"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(1538..>1811)
                     /locus_tag="PLP95_12780"
     CDS             complement(1538..>1811)
                     /locus_tag="PLP95_12780"
                     /inference="COORDINATES: protein motif:HMM:NF014801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HPB46724.1"
                     /translation="NDLFVQLVADITGRTVEVSPVRDATAIGAAMLAGLALGVWSSPE
                     ACTRLFKPRSVVLPDPDLDREAMRRQWAKAIDRARGWYEDLSALDF"
BASE COUNT          268 a          612 c          615 g          316 t
ORIGIN      
        1 caccagctcc gtgaggcgac gatcgtggcc gaacggatcg ttgcggtgct cgacgagccg
       61 atgctgatcg ctgggcgttg ggtgagcgcc agcgcgtcga tcggggtcgc ctacggggac
      121 cgcacgatga gcgtctcgga cctgttgcga aacgccgacc tcgccatgta caccgccaag
      181 aaacagggca agtcctgcta tcgggtctac gaaccggaga tgcactcggc ggcactcgag
      241 cgtctcgagg tggaggccac ccttcgcggg gccgccgccc gcggtgagct gttcgtcgag
      301 tttcagccca tctacgacac ccgcagcggg gagatggtcg aagccgaggc cctcgttcga
      361 tggaaccatc ccgaacgagg tctgctcggc cccggggcat tcatccccca ggccgagtcg
      421 acgggggtca tcgaggaagt cggcgagttc gtcttgcggg aatcgcttcg atgggttcgc
      481 gagtggggtc gcgaccttgg cctcgacgcg gtgccggccg tgaacgtgaa cctgtcgcct
      541 cgacagttga acgacccccg gctgccggaa cgtgtcttcg cggtgttgtc cgaactcgac
      601 ctgccggcat cgaagctggt gctggagatc accgagagcg ccatcatggg cgaccccgag
      661 cagtcgcgtc ggatccttga gcggatcagc gcgctcggtg tgcgccttgc ggtggacgat
      721 ttcggcaccg ggtactcgtc gttggcgtac ctgcagcagt ttccgatcgc cttcttgaag
      781 atcgacggca gtttcgtgca acagatgctc gtgaacgagg gggcatcgat ggtggaggcg
      841 gtcacccagc tggcgcacac gctgggcctg accccggtcg ccgagggtgt cgagagcgag
      901 gcccagctgc gcgaggtcgt caggatcggc gtcgacctgg cacagggata ccacctgagc
      961 cgacccgtca gccctgatgc gctgttcgcg ctgttgaagt cgcagcgacg ctccgacgcg
     1021 ctcagctcgc tcaccctgct caatccgccg accccgctcg actcgctgta gcggcggtac
     1081 ttcgggcgtt tcggcccgcc gaccctgccg gccacggcga gggtgtggtg ctggtgagcc
     1141 gccctgtaag cggggttctg ttccccggcg cctcgcggca tccggttcgg tgaccatcca
     1201 tctgtgcagc ccacctgtgg gcggcgcggt tgcgccgagc gagcaactca acccacgact
     1261 tggccttgct ccggatgggg tttaccgagc cgccacggtc gcccgcgacg ctggtgcgct
     1321 cttaccgcac cctttcaccc tcacctgtgc cgggcgaacc cggccatcgg cggtctgctt
     1381 tctgttgcac tgatccgtga ggtcgccccc acctggctct cgccagcatc ctgctcttcg
     1441 gagccccgac tttcctcagg cgcgccgatc gctcgacggc ccgcggtcac ccgggcaact
     1501 caccagcccg aggagcttag gtcagcgggc tggtggctca gaagtcgagt gccgacaggt
     1561 cctcgtacca gccgcgtgcc cggtcgatcg ccttggccca ctggcgccgc atcgcctctc
     1621 ggtcgaggtc ggggtcgggc aacacgacgc tgcgcggctt gaacagtcgg gtgcacgctt
     1681 cgggcgagga ccacaccccg agggcgagcc cggcgagcat cgcggccccg atagcggtgg
     1741 catcgcgcac cggactcacc tcgacggtgc gcccggtgat gtcggccacg agctggacga
     1801 agaggtcgtt g
//
LOCUS       DAOOMA010000582         1807 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_83070_length_1807_cov_4.138128, whole genome shotgun sequence.
ACCESSION   DAOOMA010000582 DAOOMA010000000
VERSION     DAOOMA010000582.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 1807)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1807)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1807
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_83070_length_1807_cov_4.138128"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1725
                     /locus_tag="PLP95_12785"
     CDS             <1..1725
                     /locus_tag="PLP95_12785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB46725.1"
                     /translation="SPITSGRDHDHWLSENESVGGANGSAAGGPNESVGSNGSLTWTF
                     AKLKFVLLRASLRRRQSVWNRVGTFVALLASLLGAIAGFIATVSLRGGWSAVAEERFL
                     VIGCFLVIFGWWFGPVLSGGSDETVDPMRLALLPLTRAQLRRGQIAAGLVGPAPLMVV
                     VWGIGLVIGTAGPWWTVPFSILAAVVLVLTALIGSRAIATSLAVLNRTRRGGDLATFV
                     AVLGAAMLFVAGQALQFISDEQLDTMTRIARATPLGMIGDAFRLLGDGDVVGALWRIA
                     ALGAVAAAAAWCWSRQLDRLMSESDVRGRLEAAATISALPIFDGFRSRLAHTPAGAAV
                     AREIVYLKRSPGRRASMLTSLMLGFAYVAMLAARGQIRDPMIVLVSPLAMVFALQYAA
                     NQNGVDPAAFWIEVVAGPQQIARWAGRQALGAVSILTPVLITGVVLAAVSGGWRQFAV
                     MVATMIGATFALVGLGSAMSPAFATPVPDSGNPFGNRQAMQGTGCVAGLVGIMYLVVG
                     AAIVALPEISLWWAVDNRRWGLAALVFIGALAAQLGVWFASSRFAIRSLARNELVVLE
                     RLDARLNA"
BASE COUNT          218 a          601 c          647 g          341 t
ORIGIN      
        1 tctccgataa ccagtggtcg tgatcacgac cactggttat cggagaacga gtcggtgggg
       61 ggtgcgaacg ggtcggcggc ggggggcccg aacgagtcgg tgggctcgaa cgggtcgctc
      121 acctggacct tcgccaagct gaagttcgtc ctgttgcggg cctcgctgcg gcggcgacaa
      181 agcgtgtgga atcgcgtcgg cacgttcgtc gcgctcctgg cgtcgctgct gggggcgatc
      241 gccggcttca tcgcgaccgt gtcgttgcgc ggtggatggt cggccgtcgc cgaggagcgg
      301 ttcctcgtca tcggctgctt tctcgtcatc ttcggctggt ggttcggccc cgtcctcagc
      361 ggcggctccg acgaaacggt cgacccgatg cgcctcgcgt tgttgcccct cacgcgtgcg
      421 cagttgcggc gcggtcagat cgccgccggg ctcgtcgggc cggctccgct gatggtggtc
      481 gtgtggggga tcggcctggt catcggcacc gccggcccct ggtggacggt gcccttttcg
      541 atcctcgccg cggtggtcct cgttttgacg gcgctcatcg gcagccgcgc catcgctaca
      601 tcgctcgcgg tgctgaaccg cacccgccgt ggcggcgatc ttgcgacctt cgtggcggtg
      661 ctcggagcgg cgatgttgtt cgtcgccggg caggcgctgc agttcatctc cgacgagcaa
      721 ctcgacacga tgacgcggat cgcgagagcg acacccctcg gcatgatcgg cgacgccttc
      781 cggctgctcg gcgacggcga cgtcgtgggc gcgctgtggc gcatcgccgc gctcggtgcc
      841 gtcgccgcag cagcggcgtg gtgctggagt cgccaactcg accgcctgat gtcggaatcg
      901 gatgtgcgcg gacgcctcga ggccgccgcc acgatcagcg cattgccgat cttcgacggc
      961 ttcaggtctc gtctggccca caccccggcg ggagcggccg tcgccaggga gatcgtctat
     1021 ctcaaacgtt cgcccggccg aagggcgtcc atgctcacct cgctcatgtt ggggttcgcc
     1081 tacgtggcga tgctcgcggc gcgcggccag atccgcgatc cgatgatcgt gctcgtctcg
     1141 ccgctcgcca tggtgttcgc gctgcagtac gccgcgaacc agaacggcgt cgacccggcc
     1201 gcgttctgga tcgaggtcgt ggcgggacct caacagatcg cccgctgggc cggacggcag
     1261 gcgttggggg cggtgtcgat cctgacgccg gtactcatca ccggtgttgt gctggccgcg
     1321 gtgtcgggtg ggtggcgaca gttcgcggtg atggtcgcca cgatgatcgg agcgacgttc
     1381 gcgctcgtcg ggctcgggtc cgccatgtcg ccggcattcg ccacgccggt gcccgacagc
     1441 ggcaacccgt tcggcaaccg acaggccatg cagggcacgg gatgcgtggc tggcctcgtc
     1501 ggcatcatgt atctggtggt cggggcggcg atcgtggcgc tgccggagat ctcgctgtgg
     1561 tgggcggtcg acaaccgccg gtggggcctc gccgcgctcg tgttcatcgg ggcgttggcg
     1621 gcacagctcg gcgtgtggtt cgcgtcgtcg cgctttgcca tcaggtcgct ggcgcgcaac
     1681 gagctcgtcg tgctcgaacg cctcgacgcc cgcctcaacg cctgaccgcc ctcagccgcc
     1741 ctcagccgcc ctcagcctgt tctccgataa ccagtggtcg tgatcacgac cactggttat
     1801 cggagaa
//
LOCUS       DAOOMA010000583         1806 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_83158_length_1806_cov_3.471159, whole genome shotgun sequence.
ACCESSION   DAOOMA010000583 DAOOMA010000000
VERSION     DAOOMA010000583.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 1806)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1806)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1806
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_83158_length_1806_cov_3.471159"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(186..827)
                     /locus_tag="PLP95_12790"
     CDS             complement(186..827)
                     /locus_tag="PLP95_12790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB46726.1"
                     /translation="MPRSFAHRRRDSLRFNWSVLRHRLGRSAVFYWLAVAALGIVTYL
                     AATGNTPGDQATRQVIIATAPVDPGDTLSPADIAVVAVAEAAVPDDALDDYPDGSTPG
                     SPIRVGVGLQRGDIVTQRHLSRAESELAAMVGDGRTAVSIDHDNEALPITVNDVVDLY
                     SVDTGGSIRNVARSVAIVSTSPRHLVAAVPNARVPEIISAQLGGVLTVALSGQ"
     gene            complement(919..>1806)
                     /gene="map"
                     /locus_tag="PLP95_12795"
     CDS             complement(919..>1806)
                     /gene="map"
                     /locus_tag="PLP95_12795"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HPB46727.1"
                     /translation="PSPPVTPGLQSPRRSVPASIPRPEYALTGEPNSREGRAVRTPEE
                     IEAMRVAGRVAAEVLIEVGHAVKPGVTTDELDRIGHEASIARGAYPSPLNYRGFPKSL
                     CSSVNEIICHGIPDSRKLVDGDIVNIDVTCFIEGVHGDTNCTFTVGDVDDASLELIRA
                     TRKAMHAGIDVVKPGARINEIGRAIEASVRGGPYGVVREFIGHGIGDQFHTSLQIPHY
                     YSSHYRTALEEGMTFTIEPMITLGNPALYVWDDDWTAATISGQRCAQFEHTMVVTANG
                     VELLTVPADQPPAEEQFAV"
BASE COUNT          284 a          537 c          671 g          314 t
ORIGIN      
        1 tcgacgatat cgagcagcag gcgctgcagg gtcgaagcga ccatcgcgtc gaccctgcag
       61 cgcctgctgc tcgatatcgt cgaggtcggc gcagacctga cctggacacc gggtggtgat
      121 gcggcggcat cgttggtcat cgcggacgtg tcgctcggcg ggcggtgacg gcgggaccgg
      181 aggccctatt gcccggacag cgcgacggtg agaacgccgc cgagttgtgc cgaaatgatc
      241 tcggggaccc gggcattggg caccgctgcg acgaggtgtc gcggtgaggt cgacacgatg
      301 gcaaccgatc gtgcgacgtt gcggatggac ccgccggtat ccaccgaata caggtcgacg
      361 acgtcgttga ccgtgatcgg cagtgcctcg ttgtcgtggt cgatcgaaac cgcggtgcgc
      421 ccgtcgccga ccatcgcggc gagttcggat tcggcacggc tcaggtggcg ttgggtgacg
      481 atgtccccgc gctgaaggcc gaccccgacg cggatggggg atccgggtgt ggaaccatct
      541 gggtagtcgt cgagggcgtc gtcgggcacc gcggcctcgg caacggcgac gacggcgatg
      601 tcggcgggag acaacgtgtc tccggggtcg accggggcgg tcgcgatgat cacctgtcga
      661 gtcgcttgat cgccgggggt gttcccagtc gcagcgaggt aggtgacgat gccgagcgca
      721 gctactgcca accagtagaa gaccgccgat cggccgagtc ggtggcgtag caccgaccag
      781 ttgaagcgca gcgagtcgcg gcgccggtgg gcgaacgacc tgggcatgga atcctccggg
      841 tgtgtctggt ggggaggaca ccgaagcggg tgtcgaacgc agttggtcga cctcggcggt
      901 cggaccggag gccgtcggtc agacggcgaa ctgttcctcg gcgggcggtt gatcggcggg
      961 cacggtgagc agttcgaccc cattcgcggt cacgaccatg gtgtgttcga actgggcaca
     1021 gcgctgaccg gagatcgtgg cagcggtcca gtcgtcgtcc cacacgtaga gcgcggggtt
     1081 gccgagggtg atcatcggct cgatcgtgaa ggtcattccc tcctcgagcg cggtgcggta
     1141 atgcgaggag tagtagtggg gaatctgcag tgaggtgtgg aactgatctc cgatgccgtg
     1201 gccgatgaac tccctcacga cgccgtaggg gccaccgcga accgaggctt cgatcgctcg
     1261 gccgatttcg ttgatccgtg ctccgggctt cacgacgtcg atgccggcgt gcatcgcctt
     1321 gcgcgtcgcc cggatgagtt cgagggatgc gtcgtcgacg tcgccgacgg tgaacgtgca
     1381 gttcgtgtcg ccgtggaccc cttcgatgaa acacgtcacg tcgatgttca cgatgtcgcc
     1441 gtcgacaagc ttgcgactat cggggatgcc gtggcagatg atctcgttca ccgaagagca
     1501 caacgacttg gggaacccgc ggtagttgag cgggctcggg taggcgccgc gggcgatcga
     1561 cgcttcgtgg ccgatccggt cgagctcgtc ggtggtcacc cccggcttca cggcgtggcc
     1621 gacttcgata agcacttcgg cggcgacacg gccagcgacc cgcatcgcct cgatctcctc
     1681 gggggttcgc actgcccggc cttcgcgcga gttcggctcg ccggtgagcg cgtactcggg
     1741 ccgtgggatc gacgcgggca cgctgcgacg tgggctctgc aatccagggg tcaccggggg
     1801 tgacgg
//
LOCUS       DAOOMA010000584         1802 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_83432_length_1802_cov_3.231254, whole genome shotgun sequence.
ACCESSION   DAOOMA010000584 DAOOMA010000000
VERSION     DAOOMA010000584.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 1802)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1802)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1802
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_83432_length_1802_cov_3.231254"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1082
                     /locus_tag="PLP95_12800"
     CDS             <1..1082
                     /locus_tag="PLP95_12800"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: protein motif:HMM:NF005899.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HPB46728.1"
                     /translation="FTGQVLSTGVTIIGKSRLPEFGLNASTEFMTREPTRNPWNPAYS
                     VGASSGGSAALVAAGVVPIAHANDGGGSIRIPAACAGLVGLKTTRGRHLISDRMKKVP
                     IDIGTEGIVSRSVRDTAVFLDGIEAGYRNPMLPPVGRIEGPGNRQLRVGVIFDSITGP
                     PDADTRAAVEQTVAILEEAGHHVEEIPLPVGLGFRDDFLQYWALLAHALIDLGKHLVA
                     SDFDKTKADGVGRGLRDHHRANLRHTRGAIKRLKQVPAAYGEMFRGHELVVSPVLRHP
                     APEIGYLSPQLPFSTLIDRLFAYVAYTPLNNIAGTPAMSVPARLSSTGLPVGVQLQGA
                     WGDERTLIEAAYLLEQANPFPRITD"
     gene            complement(1121..>1802)
                     /locus_tag="PLP95_12805"
     CDS             complement(1121..>1802)
                     /locus_tag="PLP95_12805"
                     /inference="COORDINATES: protein motif:HMM:NF016115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004061 - arylformamidase activity
                     [Evidence IEA];
                     GO_process: GO:0019441 - tryptophan catabolic process to
                     kynurenine [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HPB46729.1"
                     /translation="EFDADGDGVDEPVFYNGYRAGIDLVGPSEDARGDGSGHRCFAHH
                     LGLEHMAAHGVQGRGVLVDLEAHLSDEWRAVDLATLQRIMDEDGVVVEPGDILLLHTG
                     FATRILEWGGNPDPRKLFTTSSYLDAKDPALLEWIETSQISALVADNYAVEGLLGKDR
                     EPERHSFLPIHHLCLFKLGVPLGEMWYLHDLALWLREHNRSRFLLTAPPLRLPGVVGS
                     PLTPIATV"
BASE COUNT          284 a          611 c          611 g          296 t
ORIGIN      
        1 cgttcaccgg acaggtcctg tcgaccgggg tcaccatcat cggcaagtcg cggcttcccg
       61 agttcgggct caacgcctcg acggagttca tgacgcgcga gccgacccgt aacccgtgga
      121 atcccgccta ttcggtgggt gcttcctcgg gaggttcggc agcgctcgtc gcggctggag
      181 tcgtgccgat cgcgcacgcg aatgacggcg gtggttcgat ccgcatcccg gcagcgtgtg
      241 cggggctcgt cgggttgaag acgacccgcg gccgccacct catcagcgac cggatgaaga
      301 aggtgccgat cgacatcgga accgagggca tcgtgtctcg gtcggtgcgc gacacggcgg
      361 tgttcctcga cgggatcgag gccggctacc gcaacccgat gctgccgccc gtgggacgca
      421 tcgaagggcc cggcaaccgg cagctgcgtg tcggggtcat cttcgattcc atcaccggtc
      481 cgcccgacgc cgacacccgc gccgccgtcg agcagaccgt ggcgatcctc gaggaagccg
      541 gccaccacgt cgaggaaatc ccgctgcctg tcggcctcgg tttccgcgac gacttcctgc
      601 agtactgggc gctgctggcc cacgcgctca tcgatctcgg caagcacctg gtcgcatcgg
      661 atttcgacaa gacgaaggcc gacggtgtgg ggcgcggcct acgggatcat caccgggcga
      721 acctgcgcca cacccgtggg gcgatcaagc gcctgaagca ggttccggcc gcgtacggcg
      781 agatgtttcg gggccacgaa ctcgtcgtgt cgccggtgct tcgccatccc gctcccgaga
      841 tcggctacct gagcccccaa ctcccgttct cgacgctgat cgaccggctg ttcgcctatg
      901 tcgcctacac gccgctcaac aacatcgccg gaacgccggc catgtcggtc ccggcgaggt
      961 tgtcgtcgac gggcctgccg gttggcgtgc agttgcaggg ggcgtggggc gatgagcgca
     1021 cgctgatcga ggccgcgtac ctgttggaac aagccaaccc gttcccccgc atcaccgact
     1081 gagccggtcg ccgtctcgcc gtctcgccgt ctcggttgcg tcagacggtg gcgatggggg
     1141 tgagcggcga gccgacgacg ccgggcaggc gcagcggggg agcggtgagc aggaaccggc
     1201 ttcggttgtg ctcgcgcagc cacagcgcca ggtcgtgcag gtaccacatc tcgccgaggg
     1261 gcacgccgag tttgaacagg cacaggtgat gaatgggcag gaacgagtgg cgttcgggct
     1321 cgcggtcctt acccagcagt ccctccacgg cgtagttgtc ggcgacgagt gcggagatct
     1381 gagaagtctc gatccactcc aacaacgccg ggtccttcgc gtcgagatag ctcgaggtcg
     1441 tgaacagctt gcgggggtcg gggttgccgc cccattcgag gatccgcgtg gcgaaaccgg
     1501 tgtggagcaa caagatgtcg ccgggttcga cgaccacgcc gtcctcgtcc atgatccgct
     1561 gcagggtggc caggtcgacc gcgcgccatt cgtcgctcag gtgcgcttcg agatcgacga
     1621 gcacacctcg cccctgcacc ccgtgcgccg ccatgtgctc caacccgagg tgatgggcga
     1681 aacaacggtg gccgctgccg tcgccgcggg cgtcctcact cggcccgacg aggtcgatgc
     1741 ccgcccggta gccgttgtag aagaccggct cgtcgacccc gtcgccatcc gcgtcgaact
     1801 cg
//
LOCUS       DAOOMA010000585         1802 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_83439_length_1802_cov_3.105323, whole genome shotgun sequence.
ACCESSION   DAOOMA010000585 DAOOMA010000000
VERSION     DAOOMA010000585.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 1802)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1802)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1802
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_83439_length_1802_cov_3.105323"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1132)
                     /locus_tag="PLP95_12810"
     CDS             complement(<1..1132)
                     /locus_tag="PLP95_12810"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HPB46730.1"
                     /translation="MSHHVSARSGVEPFHVMEVVAAAAARGDVVHLEVGQPSDGAPPS
                     AVARAQELLASGDALGYTPALGITALRERIAGWYHERYGLETDPGRIAVTAGASGAVM
                     LALLARFDPGQRIAYAVPGYPCYPQLIAALGLIGVPVPCGAADGFVLGAEPLDRLIGS
                     DVAPIDGVFVASPANPTGTQYDPEQLRQLAQWCRDRGVQLIADELYHGITFDRPAPSA
                     GGDESAIVIGSFSKYFCMTGWRLGWMLGPAPLIDAVDRLSQHAYLSPPTLAQHIAIAA
                     FDDVAVLDARVERYAHNRRILLAALSDLGVEDVAPCDGAFYAYGRVDHWGIDSFELVQ
                     RWLAEIGVAAAPGVDFDHRDGHRWVRWSFAGSTEDVTEGCERL"
     gene            complement(1142..>1802)
                     /locus_tag="PLP95_12815"
     CDS             complement(1142..>1802)
                     /locus_tag="PLP95_12815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB46731.1"
                     /translation="AAGDADHGFHGMDFYFVGRGGMLGDVEPAVAAAAFGFFPPAVVE
                     LMWASGTSVMAASQASREYLAACAKWGRNHFGADIDYAELAQLSARVVAAGPTAGFPL
                     FAGWQKAAASVSTDDAKGAAMVLLTALRELRGGAHLVAVTAAGVEPLDAVLHTGGVPN
                     AQLFGYAEPYPDVSQLGETMEAIERTTNRIVSLAYEALDSSELDRFVDLTKAASIGIT
                     D"
BASE COUNT          307 a          617 c          620 g          258 t
ORIGIN      
        1 ggagccgctc gcagccttcg gtgacgtcct ctgtcgaacc ggcgaaggac caccggaccc
       61 agcggtgccc gtcgcggtga tcgaaatcga ccccgggcgc tgcggccaca ccgatctcgg
      121 ccagccaccg ttgcaccagt tcgaagctgt cgatccccca gtggtcgacg cggccgtagg
      181 cataaaaggc gccgtcgcac ggggcgacgt cttcgacgcc gaggtcgctc agcgccgcga
      241 gcagaatgcg gcggttgtgc gcgtagcgct cgacccgtgc atcgagcacc gcgacgtcgt
      301 cgaacgcggc gatcgcgatg tgttgggcga gggttggcgg cgacagatac gcgtgctggc
      361 tcagccggtc gacggcgtcg atcagcggtg ccggtccgag catccaaccg agccgccatc
      421 cggtcataca gaagtatttc gagaagctcc cgatcacgat cgcggattcg tcgccgccag
      481 cagagggggc cggtcgatcg aaggtgattc cgtgatacag ctcgtccgcg atgagctgaa
      541 ccccgcgatc ccggcaccac tgcgccagtt gtcggagctg ttcggggtcg tactgggtgc
      601 cggtcgggtt cgccgggctg gcgacgaaca ccccatcgat cggggcgaca tccgagccga
      661 tcaaacggtc gagcggctcg gcgccaagga cgaacccgtc cgccgctcca cacggcaccg
      721 gaaccccgat caggccgagc gcggcgatga gctgggggta acagggatac ccgggtaccg
      781 cataggcaat ccgctgtccc gggtcgaaac gggccagcag cgccaacatg accgccccgg
      841 aggccccggc ggtgacggcg atgcgccccg ggtcggtttc gaggccgtag cgctcgtgat
      901 accacccagc gatccgctcg cgaagcgcgg tgatgccaag tgccggcgtg tagcccagag
      961 cgtcgcccga tgcgagcaac tcctgagccc gtgccaccgc cgagggcggg gcgccgtcgc
     1021 tcggctggcc cacctcgaga tgcaccacgt ctccgcgcgc cgctgcggca gcgaccacct
     1081 ccatgacatg gaacggttca acgccggagc gggcggagac gtgatggctc atcgtcgggt
     1141 gtcagtcggt gatcccgata ctcgccgcct tcgtcagatc gacgaaacgg tccaattcgg
     1201 acgagtcgag cgcttcatag gccaacgaaa cgatgcggtt ggtggtgcgc tcgatggcct
     1261 ccatcgtttc gccgagttgg gagacgtcgg ggtaaggctc ggcgtagccg aacaactggg
     1321 cgttgggaac accacccgtg tgcaacacgg catcgagcgg ctcgaccccg gcggcggtga
     1381 cggccacgag gtgggcgcct ccgcgcagct cacgcagcgc ggtgagcaac accatggcgg
     1441 cgcccttcgc atcgtcggtc gacaccgacg ctgcggcctt ttgccacccg gcaaacagcg
     1501 ggaacccagc ggttgggccg gcggccacga cgcgggcgct caactgggcc agttccgcgt
     1561 agtcgatgtc ggcgccgaag tggttgcgac cccatttcgc gcaggccgcc aggtattcgc
     1621 gcgaggcctg gctcgccgcc atcaccgagg tgccgctcgc ccacatcaac tcgacgacgg
     1681 ccggcgggaa gaacccgaag gccgctgccg ccactgcggg ctcgacgtcg ccgagcatcc
     1741 cgccgcggcc aacgaagtag aaatccatgc cgtggaaccc gtggtcggcg tcgcctgcgg
     1801 cg
//
LOCUS       DAOOMA010000586         1798 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_83703_length_1798_cov_4.273092, whole genome shotgun sequence.
ACCESSION   DAOOMA010000586 DAOOMA010000000
VERSION     DAOOMA010000586.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 1798)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1798)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1798
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_83703_length_1798_cov_4.273092"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            255..734
                     /locus_tag="PLP95_12820"
     CDS             255..734
                     /locus_tag="PLP95_12820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB46732.1"
                     /translation="MNMLAAIGSTGYNVLLVLHLLAMAVWFGANVAIGVTHGRAVGAS
                     NEIIGWLAGVQRMVSMRIKSAAFLVLFVTGMGLVGMSKEIFKMSDPFVSVGFFAIILG
                     GALGGMVYAPGSRAVEAAAKDGDAAAIKRHNDKMGLIGMVESLVVVITVLFMVFKWG"
     gene            complement(758..1159)
                     /locus_tag="PLP95_12825"
     CDS             complement(758..1159)
                     /locus_tag="PLP95_12825"
                     /inference="COORDINATES: protein motif:HMM:NF013970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HPB46733.1"
                     /translation="MLDTTALLARYLDGPFRPIVLDAMRSDDDWCACALALSEALMLI
                     DQIGDDPERADVLRRTLRDDWERIHVVPVDQRCLDRASELGRTQPLRTIDALHLAAAD
                     RLPRPVRFATFDPAQIPVALALGFDVVSTES"
     gene            complement(1165..1443)
                     /locus_tag="PLP95_12830"
     CDS             complement(1165..1443)
                     /locus_tag="PLP95_12830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB46734.1"
                     /translation="MERIGVRDLRADLSAHLRRAGAGERIVVTIDGTPVAQLGPLEAD
                     LDHLTIDDLAAKGLIIAARRSDRPEPEFVMPMWAGTRLDQLVREVRGR"
     gene            1547..>1798
                     /locus_tag="PLP95_12835"
     CDS             1547..>1798
                     /locus_tag="PLP95_12835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HPB46735.1"
                     /translation="MNFAFSEEQEELRKIVRDFLNAKSSEATVRELMETESGYDPAVW
                     SQLAEQLGVQSLIIPEEFGGQGFGYVELIVVLEEMGRRLL"
BASE COUNT          320 a          544 c          605 g          329 t
ORIGIN      
        1 tcgttggcgg catcgaagag cagcgcctcg ccggtgtggc gacaccgcag aacgaacacg
       61 ttgttctcga acgggccgac gacgaccttg tgaatctcaa gatcggtgtc ggcgtggtgc
      121 agcgtggtgt cggccatggg tccatgttgc cccaaatggc ggcgtagagc cgggcggaac
      181 cgatgtggtt gcatcggcac cgcaagcgag ggtggtaggc gatgtcgtgg gccgggcatc
      241 taggctgagc tgccatgaac atgctcgcag ctattggttc gaccggttac aacgtcttgt
      301 tggtccttca ccttctcgcg atggcggtct ggttcggggc gaacgtcgcc atcggcgtga
      361 cccacgggcg ggccgtcggg gcatcgaacg agatcatcgg ctggctcgct ggtgttcagc
      421 gcatggtgtc gatgcgcatc aagagcgcgg cgtttctcgt gctcttcgtc accggcatgg
      481 gcctcgtggg catgagcaag gaaattttca agatgtccga cccgttcgtg tcggttggat
      541 tcttcgccat catcctcggc ggagcgctcg gtgggatggt gtatgccccc ggctcccggg
      601 cggtcgaggc cgctgcgaag gacggcgacg ctgcggcgat caagcgtcac aacgacaaga
      661 tgggactcat cggcatggtc gagtcgctcg tcgtcgtgat caccgtgttg ttcatggtgt
      721 tcaagtgggg ttgatcagct gcggctgagc accagtccta cgactcggtc gacaccacat
      781 cgaagccgag ggccaacgcg acgggaatct gcgcggggtc gaacgtcgcg aaccggaccg
      841 gtcgtggaag tcgatccgcg gccgcaaggt gaagcgcgtc aatcgtgcgc aacggctggg
      901 tccgacccaa ctcgctcgct cggtcgaggc accgctggtc gaccggtacg acgtggatgc
      961 gctcccaatc gtcgcgcagg gtgcgccgta agacatccgc ccgttcgggg tcgtcaccga
     1021 tctgatcgat cagcatcaat gcctcgctga gggccaacgc acacgcgcac cagtcgtcgt
     1081 cggagcgcat cgcgtccagc acgatcggac gaaacgggcc gtcgagatag cgcgccaaca
     1141 acgccgtggt gtccaacatg agcgtcaccg gccgcgcacc tcacgcacga gttgatcgag
     1201 gcgcgtcccc gcccacatgg gcatgacgaa ctccggttcg ggccgatcgc ttcgccgtgc
     1261 cgcgatgatg agccccttgg cggcgagatc gtcgatggtc aggtggtcga ggtcggcctc
     1321 aagcggtccg agttgggcga ccggcgtgcc gtcgatcgtc acgacgatac gttcgcccgc
     1381 tccggcccga cgcaggtggg ccgaaagatc ggcgcgtaga tcgcgtaccc cgatgcgttc
     1441 catcgccgga ccttagcgaa gccctggcga cgatgtacag cgatgatgta catcagtgta
     1501 caccccgccg attgtgccgt cggccaacac gccacagaat gtcgccatga atttcgcctt
     1561 ctcagaagaa caagaagaac tgcgcaagat cgttcgcgac ttcctcaacg cgaagtcctc
     1621 cgaggcgacc gtgcgtgagc tcatggaaac cgagtcgggc tatgacccgg cggtgtggag
     1681 ccagctcgcc gaacagctcg gcgtgcagag cctgatcatc cctgaggagt tcggcgggca
     1741 ggggttcggg tacgtcgaac tcatcgtcgt gctcgaggag atgggacgcc gcctgttg
//
LOCUS       DAOOMA010000587         1798 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_83733_length_1798_cov_2.868044, whole genome shotgun sequence.
ACCESSION   DAOOMA010000587 DAOOMA010000000
VERSION     DAOOMA010000587.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 1798)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1798)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1798
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_83733_length_1798_cov_2.868044"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..684
                     /locus_tag="PLP95_12840"
     CDS             <1..684
                     /locus_tag="PLP95_12840"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HPB46736.1"
                     /translation="WAQGLGGTVRLNNLAARWNPYLRGERLEADATFRTPLGDPLPGS
                     KKLWSHIRRCRRRLVEDGATIDVRVGSQIRPDDLEQLLALHARRSHDAGRTTLFTEAQ
                     RPFFEGLARDARPGAGPVVVRIDHGDKVIGALIGFADSETFAYYQSGWDSEYASLSIG
                     SLLVATAIEYAASESLKTFDFLRGDEPYKLRFDARQVDNYSITAYRGAGGRVLRLRDD
                     ARARRRRSS"
     gene            785..>1798
                     /locus_tag="PLP95_12845"
     CDS             785..>1798
                     /locus_tag="PLP95_12845"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HPB46737.1"
                     /translation="MSQSTWVSRAPRLRHVAPFDGVRGLGVLGVMGGHALPLATHSFS
                     AIVDVFFVISGFLITTLLLQEHRQTGGIAVRKFYARRLLRLLPGLWVMLAGTAVIGVI
                     IKVSGRLDDPALHLGKPTLKALGKEIVVSALYLHNIVYPTNNGPWIDHLWTLSVEEQF
                     YLLIGLASLIFIVRGHIKAITVVLVALTAAIQISRFALTPGPLGQVAAAVWVQRPDSL
                     MVGMLAAIVSANIADPVPPRVKKALSVAGWVGIPMLFGAVWASTGWARTLGIHHAYVP
                     ENYGDLLAAGTRPTGFYWIQWGNTVANWGVAAITLCLFRVPDWWPNKLMSWKLLVWVG
                     GALS"
BASE COUNT          275 a          580 c          606 g          337 t
ORIGIN      
        1 tgggcccagg ggctgggagg aacggttcgg ttgaacaacc tcgcggcgcg gtggaatccg
       61 tatttgcgcg gcgagcgcct ggaagccgac gccacgtttc gaaccccgct cggcgacccg
      121 ttgcccgggt cgaagaaact gtggagccac atccgtcggt gccgccgacg tctcgtcgag
      181 gacggcgcaa cgatcgacgt gcgcgtcggg tcacagatcc gccccgacga cctcgaacag
      241 ctgttggcac tgcacgctcg acggtcccac gacgccgggc gtacgaccct gttcaccgag
      301 gcgcaacgtc cattcttcga ggggttggcc cgtgacgccc ggcccggagc gggcccggtc
      361 gtggtgcgga tcgaccacgg ggacaaggtc atcggagcgt tgatcggctt cgccgactcc
      421 gagaccttcg cctactacca atcggggtgg gactcggagt acgccagttt gagcatcggc
      481 tcgttgctcg tcgccaccgc catcgaatac gcagcctcgg aatcgctgaa gaccttcgat
      541 ttcctccgcg gggatgagcc ctacaagctg cgtttcgacg ctcgacaggt cgacaactac
      601 tcgatcaccg cctaccgcgg cgccggtggg cgagtcctgc gccttcgcga cgacgcacgg
      661 gcccggcgcc ggcgatcgtc ctgatggcct cgagctgatg gcctcgaggc cgtcgagagc
      721 gcggcgcacc ccctaagatg gatcagcccg tggtcgcccg cggggccgtg aacgaggggg
      781 agcattgtca caatcgacgt gggtctcaag agcgccgcga ctgcgccatg tcgccccatt
      841 cgacggcgtt cggggcctcg gagttctcgg cgtcatgggc ggacacgccc tgccgcttgc
      901 gacccactcg ttcagcgcga tcgtcgacgt gttcttcgtg atctcggggt ttctgatcac
      961 gacgttgctg ttgcaggaac accgccagac gggcggtatc gccgttcgaa agttctatgc
     1021 gcgccgtctc ctgcgcctct tgcccggcct gtgggtgatg ctcgccggaa cggcggtgat
     1081 cggcgtgatc atcaaggtgt cgggtcgcct cgatgatccg gcactgcacc tgggcaagcc
     1141 caccctcaag gcactcggca aggagatcgt cgtttccgcg ctgtacctgc acaacatcgt
     1201 gtacccgacc aacaacgggc cgtggatcga tcacctgtgg acgttgtcgg tcgaggagca
     1261 gttctacctg ctcatcgggt tggcatcgct gatctttatc gtccgcgggc acatcaaagc
     1321 catcaccgtc gtgctggtcg cgttgaccgc cgcgattcag atcagccgct tcgcgctgac
     1381 ccccggaccc ctcgggcagg tcgccgccgc ggtgtgggtt caacggccgg attcgctgat
     1441 ggtgggcatg ttggcggcga tcgtgtcggc caacatcgcc gacccggtgc cgccgcgggt
     1501 caaaaaggcg ctgtcggtgg ccggttgggt cgggattccg atgctgttcg gcgccgtgtg
     1561 ggcatccacc ggctgggccc gcaccctcgg cattcaccat gcgtacgtgc cggagaacta
     1621 cggcgacctg ttggcagcgg gcacccggcc gaccgggttc tattggatcc agtggggcaa
     1681 cacggtcgcg aactggggtg tggccgccat cacgttgtgc ctgtttcggg ttcccgactg
     1741 gtggccgaac aagttgatga gttggaagct gctggtgtgg gtgggcggcg cattgtcg
//
LOCUS       DAOOMA010000588         1797 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_83784_length_1797_cov_3.917336, whole genome shotgun sequence.
ACCESSION   DAOOMA010000588 DAOOMA010000000
VERSION     DAOOMA010000588.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 1797)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1797)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1797
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_83784_length_1797_cov_3.917336"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1095
                     /locus_tag="PLP95_12850"
     CDS             <1..1095
                     /locus_tag="PLP95_12850"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPB46738.1"
                     /translation="RNLHSAIAEQRRTTLKAAWYVGLMFPIADVFCAVALGAVIATGA
                     SVGVDWGLNAGSLVALMFLVNLLQQPIGELGEVLDQTQSAIAGWRKVLDVLDTPVDVV
                     EPVDGVRLPAGPLDIDIDHVDFSYGDGALVLNDVVLKIPAGTNLAIVGETGSGKTTLA
                     KLICRLADPTGGEIRVGGVDLRDVDAQARTHAIRMVPQDGFLFEGTVGDNILLGRTRY
                     DGRPAGALDAMAAIDALGLRWWVDSLPDGLDTHTGERGDNLSVGERQLVALARAQLAD
                     PGLLILDEATSAVDPETERALAGALLRLAQGRTTVSIAHRLSTAEAADLVAVFDRGCL
                     VEFGAHDELVAAGGVYAGLYGSWVGNTRAS"
     gene            1305..>1797
                     /locus_tag="PLP95_12855"
     CDS             1305..>1797
                     /locus_tag="PLP95_12855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB46739.1"
                     /translation="MKIDAADLAERTHGLTTTWWLRAAGMTRSEIRWLTRSGQWEDLG
                     NGVLRRKGTPRTVEQQMLAAVLLVAGRRGHAAVSYESAGWLWSLDGMRPRPIHVVTTR
                     SRVTTAPEGSRIRRMEELPARWTTQIDGITVCRPELLALHLFADFREETAIRRVEQMW
                     SKRL"
BASE COUNT          276 a          608 c          607 g          306 t
ORIGIN      
        1 cgaaacctcc actccgccat cgccgagcag cgccgcacca cgttgaaggc cgcctggtat
       61 gtggggctga tgtttccgat cgccgatgtg ttctgcgcgg tcgcgctcgg agcggtgatc
      121 gccaccgggg ccagcgtggg ggtcgactgg ggcctgaacg ccgggtcgct ggtggcgttg
      181 atgttcctcg tgaatctgtt gcagcagccg atcggcgagc tcggcgaggt gctcgaccag
      241 acccagtccg ccattgccgg gtggagaaag gtgctcgacg tgttggacac ccccgtcgac
      301 gtcgtcgagc cggttgacgg ggttcggttg ccggcgggtc cactggatat cgacatcgat
      361 catgtcgatt tctcctatgg tgacggcgcg ctcgtgttga acgacgtcgt gttgaagatc
      421 cccgccggca ccaacctggc gatcgtcggc gagaccggct cgggaaagac cacgctcgcc
      481 aaactgatct gccgtctcgc cgacccgacc ggcggcgaga tccgtgtcgg aggggtcgac
      541 cttcgagacg tcgacgccca ggctcgcacc cacgccatcc gcatggttcc ccaggacggc
      601 ttcctgtttg aaggcaccgt cggtgacaat attctgctcg gccggacccg ctacgacgga
      661 cgtcccgccg gggcactcga tgcgatggcg gcgatcgacg cgcttggact gcgatggtgg
      721 gtcgactcgt tgcccgacgg cctcgacacc cacaccggcg aacgcggcga caacctctcg
      781 gtcggcgaac ggcaactcgt agcgctggcc cgagcccagc tcgccgatcc cggcctgttg
      841 atcctcgacg aggcgacctc ggccgtcgac cccgaaaccg aacgagcgct tgccggcgcc
      901 ttgttgcgcc tcgctcaggg gagaacgacg gtgtcgatcg ctcaccggct ttccaccgcc
      961 gaggcggccg acctggtcgc ggtgttcgac cgcggatgcc tcgtcgagtt cggtgcgcat
     1021 gacgaactcg tcgcggccgg cggggtgtac gccggcctct acgggtcgtg ggtcggtaac
     1081 acccgcgcct catagccctg ccagcgctag cccgccccgc cccgcctggc cctgcctggc
     1141 cctgcctggc cctgcctgcc cgccggcccg cctgcccagc ccgacccgtt ctccgataac
     1201 cagtggtcgt gatcacgacc actggttatc ggagaacggg ccgagggtga gtagggggac
     1261 agcccccata gatgccggcc ccgccaacgg cgcacgatcg accgatgaag atcgatgcag
     1321 ctgacctggc cgagcgaacc cacgggctca caaccacctg gtggcttcgc gccgcgggaa
     1381 tgacccgatc cgagatccgc tggctcactc gttcggggca gtgggaagac ctgggcaacg
     1441 gggtgttgcg acgcaagggg acgccgcgca ccgtcgaaca gcagatgctc gctgcggtgt
     1501 tgcttgtggc cggacgtcgc ggccatgcgg cggtctccta tgagtccgct gggtggttgt
     1561 ggtcgctcga cggaatgcgc ccccgaccga ttcacgtcgt gacgacccgc agccgggtga
     1621 cgaccgcgcc tgaggggagc cgaattcgtc gcatggagga actgcccgcg cgttggacga
     1681 cgcagatcga tgggatcacc gtctgtcgcc ccgagctgtt ggccctgcac ctgttcgccg
     1741 actttcgtga ggagacggcg attcgtcgag tcgaacagat gtggtcgaaa cggctgc
//
LOCUS       DAOOMA010000589         1793 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_84076_length_1793_cov_3.241657, whole genome shotgun sequence.
ACCESSION   DAOOMA010000589 DAOOMA010000000
VERSION     DAOOMA010000589.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 1793)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1793)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1793
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_84076_length_1793_cov_3.241657"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..307
                     /locus_tag="PLP95_12860"
     CDS             <1..307
                     /locus_tag="PLP95_12860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB46740.1"
                     /translation="DLDNLVPICSFHHRRVHGGHLDITIVDGIAEFRDGRGSLLGNTL
                     ARHMVEGAPHRPSEQSLRDHIDNTAIDKYTAKPCGGGAPLTAWALDTWVEGLLYRPR"
     gene            complement(359..>1793)
                     /locus_tag="PLP95_12865"
     CDS             complement(359..>1793)
                     /locus_tag="PLP95_12865"
                     /inference="COORDINATES: protein motif:HMM:NF036799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB46741.1"
                     /translation="SQLLVAQGATLHPGVVVAEGNTPKPACFDVRTARVNAILGSSLS
                     TGEIRGYLDSIRFTTSDRGGVAGDSEVSDSEVIDSDVFAVEVPSWRPDSTAEIDAIEE
                     VARLHGYGRFGKTLPKSPTGGGLTDRQVDIRRLRRFLVATGLNEVTPMAFLAPGDAEN
                     SGVRADAVRVANPLVAEESVLRTSLLPGLVKTVGYNATHRQVGVGLFEIGHCFAAAPD
                     GGLPVEWEEVAVVLAGRDARDAVELARRMTTLFGLVGPVTIRNEPVGGLHPGRSGVIR
                     AGSKKKGADIGVVGEVDPAVAERHGISERVAYVSFTLGGPEAWHGGDGLLSVARSEAK
                     ARPISRMPSADFDLAFRVADSVGADVLGQTLRGAAPEVVSVELFDVFRGGAAGAAAGG
                     VGDAGDASGAAGDASGAAGDASGASGAAGAAGAAGAAASPGGGGRSLAYRIRVQALDH
                     TLKDAEIAAVRERLIEAASNGHGATLR"
BASE COUNT          329 a          681 c          536 g          247 t
ORIGIN      
        1 cgacctcgac aacctggtcc cgatctgctc gttccaccat cgccgtgtcc atggcgggca
       61 cctcgatatc acgatcgtcg atggcatcgc cgagttccgt gacggacgcg gaagcctgct
      121 cggcaacacg ttggcacgcc acatggtcga gggcgcaccg caccggccga gcgaacagtc
      181 cttgcgcgac cacatcgaca acacggccat cgacaagtac acggctaagc cgtgcggggg
      241 tggcgcacca ctcacagcgt gggcactcga cacctgggtt gagggcctcc tctaccgccc
      301 ccgatgaagg tggcgctgag gacgctccac actggtcatg tcgatgtgcc ggccgccgct
      361 accgcaacgt cgcgccgtgg ccgttcgatg ctgcctcgat gagccgttcg cgcaccgccg
      421 caatctcggc gtccttgagc gtgtggtcga gcgcctgcac ccggattcgg taggccagcg
      481 agcgaccacc tccaccgggt gaggccgcgg caccagcggc acccgcggca cccgcggcac
      541 ccgaggcacc cgaggcatcg cccgcggcac ccgaggcatc gcccgcggca cccgaggcat
      601 cgcccgcatc accaacgcca cccgcggcgg caccggcggc gccgccacga aagacgtcga
      661 acaactccac cgacacgacc tcgggcgcgg caccccgcaa cgtctgtccc aacacatctg
      721 cgccgaccga gtccgccacc cggaatgcga gatcgaaatc agcgctgggc atccgactga
      781 ttggacgcgc cttggcctcg ctgcgcgcca cactcaacag gccatcgcca ccgtgccagg
      841 cctcggggcc gccgagggtg aacgatacgt aggccacccg ttcggaaatc ccgtgtcgct
      901 cggcgaccgc cggatcgacc tcacccacaa ctccgatgtc ggcacccttt ttcttcgagc
      961 cggcgcggat caccccgctg cgtcccgggt gcaggccgcc gaccggctcg ttacggatgg
     1021 tgaccggacc caccaggccg aacagcgtcg tcatgcgccg tgccaactcc acggcgtctc
     1081 gcgcatcgcg accggcgagc accacggcga cctcctccca ttccaccggc aacccgccat
     1141 cgggggccgc ggcgaagcag tggccgatct cgaacagccc gacaccgacc tgacgatgtg
     1201 tcgcgttgta cccaaccgtc ttcaccagac ccggcaacag cgaggttcga agtaccgact
     1261 cctcagcgac gagcggattg gcgactcgca ccgcatcggc ccgaaccccg gagttctccg
     1321 catcaccggg cgcgaggaac gccatcgggg tgacttcgtt caacccagta gcgacgagga
     1381 accggcgcag acgacggata tcgacctgtc gatcggtgag gcctcccccg gtcggcgact
     1441 tcggcagcgt cttgccgaaa cggccatagc cgtgcagcct cgcgacctcc tcgatggcat
     1501 cgatctcggc ggtcgaatca gggcgccacg acggcacctc gaccgcgaac acatcggaat
     1561 cgatcacctc ggaatcgctc acctcggaat cacccgccac gccgccacga tccgaggtgg
     1621 tgaaccggat cgagtcgaga tacccgcgaa tctccccggt cgacaacgag gatccgagaa
     1681 ttgcgttcac ccgcgccgtt cgcacgtcga aacacgccgg cttcggcgtg ttgccctcgg
     1741 cgacgacaac gccggggtgc aacgtcgccc cctgtgcaac gagcaactgc gag
//
LOCUS       DAOOMA010000590         1790 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_84297_length_1790_cov_3.410951, whole genome shotgun sequence.
ACCESSION   DAOOMA010000590 DAOOMA010000000
VERSION     DAOOMA010000590.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 1790)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1790)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1790
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_84297_length_1790_cov_3.410951"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1790)
                     /locus_tag="PLP95_12870"
     CDS             complement(<1..>1790)
                     /locus_tag="PLP95_12870"
                     /inference="COORDINATES: protein motif:HMM:NF013508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB46742.1"
                     /translation="PSNTPGAAKANTVTGEGSYGGGIPGVPLGTASTNHVLATPGFWG
                     ANTSKSFHDASGSSNYYVTDGDTGRYQFSATIDSDYPLDRVVLSDGSWTFTDGDIAPD
                     RSGGGFPDSFTPTSITPGTWNLAIPATIETSADGITWVTAASGVASGAAAIAWPNPAH
                     RHVRWVWDESTDFSGVFSTTGQRVVGTIGTPAATDYGRYTNTSTLEVSPRDQALRTAT
                     SSAPYFLESPLPHPGIAKTVSRTTMQPGDQATYTITASNSADATGSLQQPIVTDCVPA
                     HFTVVSTTSTGWTPSVDPTCNAGETPLAFQWSGTLTPGQSATAITITVKAHDHTDGTP
                     IPYTSASVKHINTARIAPGSGGSFRHCVQGPCAASREVTVSPTIQLDSSKCVSGRLDG
                     GIFRPTPRCTSGEAAQAPALTTPGGTIDYRLSLRNVGNVDANNVTFIDILPRVGDTAV
                     ITGTPLNQRRTEYRPLLVDEIDVPAGWTVEYSTSANPCRAEVGGPSTGCDTPAWTSTP
                     DPLALSTYRSVRFSYAGTLHFGDDATFEWRMRAPVVDASYDRDGTDADDPQEFLAVCD
                     PLVAYTDDSHCPRAVNSFAYGPMRTSIRPL"
BASE COUNT          264 a          497 c          712 g          317 t
ORIGIN      
        1 cttaagggac ggatcgatgt ccgcatcggc ccataggcga aggagttcac cgctcggggg
       61 caatggctgt catcggtgta ggcgaccagc gggtcacaca ccgccaggaa ctcctgaggg
      121 tcgtctgcgt cggtgccgtc ccggtcgtag gaggcgtcga cgaccggggc gcgcatgcgc
      181 cattcgaagg tggcgtcgtc gccgaagtgc agggtgccgg cgtaggagaa ccgcaccgat
      241 cggtaggtgg acaacgccag cggatcgggg gtcgaggtcc aggcgggggt gtcgcaaccg
      301 gtgctcggac cgccgacctc tgcacgacat gggttggccg aggtcgagta ttcgaccgtc
      361 cagcccgcgg gcacatcgat ttcgtcgacc aggagcggcc ggtattcggt gcgccgttgg
      421 ttcaacggcg taccggtgat gaccgccgtg tcgccgacgc ggggtaagat gtcgatgaac
      481 gtgacgttgt tggcgtcgac gttgccgacg ttgcgaaggc tcaggcggta gtcgatggtg
      541 ccgccgggtg tggtgagcgc cggggcttgt gcggcctcac cgctcgtgca ccgcggggtc
      601 ggtcggaaga tgccgccgtc gagtcggccg ctcacgcact tgctgctgtc gagctgaatg
      661 gtcggcgaga cggtgacctc ccgagaggct gcgcacgggc cctgcacaca gtgacgaaag
      721 ctgccgccgg atcccggggc gatgcgggcg gtgttgatgt gtttgaccga tgccgaggtg
      781 taggggatcg gcgtaccgtc ggtgtggtcg tgtgccttga cggtgatggt gatggcagtg
      841 gcgctctgac ctggggtgag ggtgcccgac cattggaagg ccaacggggt ttcgccggcg
      901 ttgcaggtcg gatccaccga cggggtccat ccggttgagg tggtggagac cacggtgaag
      961 tgtgccggca cacagtcggt cacgatcggc tgctggagcg aaccggtcgc gtcggcggag
     1021 ttggacgcgg tgatggtata ggtcgcctgg tcgccgggtt gcatggtcgt gcggctgacg
     1081 gtcttggcga taccggggtg aggcagtggt gactcgagga agtacggcgc cgacgaggtg
     1141 gcggtgcgaa gcgcctgatc gcgcgggctc acctcgaggg tcgaggtgtt ggtgtatcgg
     1201 ccgtagtcgg ttgcggccgg cgttccgatg gtgccgacaa cgcgctggcc ggtggtgctg
     1261 aacactcccg agaagtcggt cgactcatcc cacacccaac ggacgtggcg atgggccggg
     1321 ttcggccagg cgatggccgc agcgccgctg gcgacgccgc tcgcggcggt cacccaggtg
     1381 atcccatcgg cggaggtttc gatcgtcgcg gggattgcga ggttccacgt gccgggggtg
     1441 atcgaggtgg gggtgaagct gtcggggaac cctccgccgg agcggtcggg cgcgatatcg
     1501 ccgtcggtga aggtccagga gccgtcggac aacacgaccc ggtcgagggg gtagtcgctg
     1561 tcgatcgtgg cgctgaactg gtaccggccg gtgtcgccgt cggtcacgta gtagttggag
     1621 gagccggagg cgtcgtggaa ggacttcgag gtgttggcac cccagaagcc gggggtggcc
     1681 aggacgtggt ttgtcgatgc ggtgccgagc ggaactccag gaatcccgcc cccgtaggaa
     1741 ccctcacccg tgacggtgtt cgccttggcc gcccccgggg tgtttgaggg
//
LOCUS       DAOOMA010000591         1787 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_84510_length_1787_cov_3.584873, whole genome shotgun sequence.
ACCESSION   DAOOMA010000591 DAOOMA010000000
VERSION     DAOOMA010000591.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 1787)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1787)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1787
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_84510_length_1787_cov_3.584873"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..890)
                     /locus_tag="PLP95_12875"
     CDS             complement(<1..890)
                     /locus_tag="PLP95_12875"
                     /inference="COORDINATES: protein motif:HMM:NF016777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HPB46743.1"
                     /translation="MTTRTLDCPVFDADHHMYETRDALTKYLPERYKGAIDYIDVRGR
                     TKIVVRGHISDYIPNPTFDVVARPGAQEEYFRVGNPEGKSRREIFGEPMRSIPAFREP
                     APRLELLDEQGIDRCLMFPTLASLVEERMRDDPELIHAVTHALNQWMHETWTFNYEDR
                     IFATPVISLPIVDKAIEELDWVVERGAKVVLIRPAPVPGFRGPRSFGLPEFDPFWERV
                     AEHDILVAMHASDSGYAQYLDDWEPADAYLPFKPTAFRMVAMGKRPIEDTMAAIVCHG
                     ALHRNPDLRVLSVENGASWVP"
     gene            1099..>1787
                     /locus_tag="PLP95_12880"
     CDS             1099..>1787
                     /locus_tag="PLP95_12880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB46744.1"
                     /translation="MADTATSAHASASPYYDPFDFDIDTNPYPVWKRLRDEAPLYYND
                     KHDFYALSRADDVEAALVDWRTYSSAKGTLLELIRADIDIPPGSIIFDDPPTHDRLRS
                     VLSRVFTPRKMNAIEPKVREFCARSLDPLVGSGGFDFIADLGAQMPMRTIGMLLGIPE
                     TDQEAIRDRVDEGLKLTDSNNAGITAEQMQENIDQAQIFADYIDWRAKNPSDDVMTEL
                     LNAEVETDDGTT"
BASE COUNT          334 a          570 c          568 g          315 t
ORIGIN      
        1 ggcacccacg acgcgccgtt ttcgacggag agcacccgca ggtccggatt gcggtgcagc
       61 gcaccatgac acactatcgc ggccatggtg tcctcgatgg gccgcttgcc catcgcgacc
      121 atccggaacg cggtcggctt gaacggcagg tacgcgtctg cgggctccca gtcgtcgagg
      181 tactgggcgt aaccgctgtc ggaggcgtgc atggccacga ggatgtcgtg ttcggcgacg
      241 cgctcccaga acgggtcgaa ctcgggaagg ccgaacgagc gtggcccgcg gaacccgggc
      301 accggcgcag gccggatgag cacgaccttg gcgccgcgct cgacgaccca gtcgagttcc
      361 tcgatcgctt tgtcgacgat gggcagcgag atgacggggg tggcgaagat gcggtcctcg
      421 tagttgaacg tccaggtctc gtgcatccac tggttcagtg cgtgggtgac ggcgtggatc
      481 agttcggggt cgtcacgcat gcgctcctcg acgaggctgg caagcgtcgg gaacatgagg
      541 cagcggtcga tgccctgctc gtcgagcagt tcgagtctcg gggccggttc gcgaaacgcc
      601 gggatcgagc gcatgggctc gccgaagatt tcgcggcggc tctttccctc ggggttgccg
      661 acccggaagt actcctcctg ggcgccgggg cgggcgacga cgtcgaaggt cgggttgggg
      721 atgtagtcgc tgatgtggcc gcgcacgaca atcttggtgc gacctcgaac gtcgatgtag
      781 tcgatggccc ccttgtagcg ctccgggagg tacttggtga gcgcgtctcg ggtttcgtac
      841 atgtggtggt ctgcgtcgaa gacgggacag tccagtgtgc gagtggtcat cgtggccccc
      901 tgatgtgttg cggccctctg gctgaggaga accccattat cgcaaacgga gagttaggtt
      961 tcaacgagca cgcgggggtt cgtcacatct gcgagccgcc agcgtcccgc ccagagtcgc
     1021 cattcgaaca cgattgcgag aatcagcttc tcctcataga gtaacttcac tttcatcgcc
     1081 gtcgtcaggg ggaccgacat ggccgacacc gccaccagcg cgcacgcttc cgcctcaccg
     1141 tattacgacc cgttcgactt cgacatcgac accaacccat atccggtgtg gaaacggctg
     1201 cgcgacgagg cgccgctcta ttacaacgac aaacacgact tctatgcgct cagccgcgcc
     1261 gatgacgtcg aggcggcact cgtcgactgg cgcacctaca gctcggcgaa aggcaccctg
     1321 ctcgagctga ttcgcgccga catcgacatt ccgccgggct cgatcatctt cgacgacccg
     1381 cccacccacg atcgcctccg cagcgtgttg tcgcgggtgt tcactccacg aaagatgaac
     1441 gcgatcgaac cgaaggtacg tgagttctgc gcccgaagcc tcgacccact cgtcggctcc
     1501 ggcggcttcg acttcatcgc agacctcggt gcccagatgc ccatgcgcac gatcggcatg
     1561 ttgttgggca tccccgaaac cgaccaggaa gcgatccgcg accgggtcga tgaagggctc
     1621 aagctcaccg attcaaataa cgccggaatc accgccgaac agatgcagga aaacatcgat
     1681 caggcgcaga tcttcgccga ctacatcgac tggcgggcca agaacccctc cgacgacgtc
     1741 atgaccgagt tgctcaacgc cgaggtcgaa accgacgacg gcaccac
//
LOCUS       DAOOMA010000592         1786 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_84578_length_1786_cov_5.324668, whole genome shotgun sequence.
ACCESSION   DAOOMA010000592 DAOOMA010000000
VERSION     DAOOMA010000592.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 1786)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1786)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1786
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_84578_length_1786_cov_5.324668"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(1..621)
                     /locus_tag="PLP95_12885"
                     /pseudo
     CDS             complement(1..621)
                     /locus_tag="PLP95_12885"
                     /inference="COORDINATES: protein motif:HMM:NF012517.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
     gene            complement(643..1470)
                     /locus_tag="PLP95_12890"
     CDS             complement(643..1470)
                     /locus_tag="PLP95_12890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB46745.1"
                     /translation="MTVTTSSPLSLRACRELAHRLISADEPTNALWAIDDPDPTSAPT
                     LVRGFDAAEDLLGLDLRGAHAVALVAQCRVSGEQGYSALVQSSCGCAAVAVRTNHDAR
                     IISGDSFDGFLSDLLRRTLGLPTMPEDRSPAEAVAAAWLTAVLDAVADPATAVYVRSW
                     ADVVAMHPFVDFTGLSHVGLAAEEAIDPELDVTVDVIARLCGELDWERLRHLVASDAE
                     PCSGLRSCEAAWMDTAMFARWTLGCYRDVEELLDDCALFISDELIERLQCALARCAQ"
BASE COUNT          326 a          579 c          614 g          267 t
ORIGIN      
        1 gccgacctcg gtgcgtcgct cgcgggcgta catgatcgtc atcccgaagt gttcgatgtc
       61 gaagggccgc ccggcgtctt cggctgaacg cagtatcacg tcgcgacagt cgctcgcctg
      121 ggccggggtc tggaagctgc cgatccatcc gtcagcgagt cgaccggtcc gctggagcgc
      181 accctcactg cgcgccccca accagatgga atgcggttcg ctcggcgtcg gcttgagccg
      241 cagcgccttg ccctcgccag gatgtggcac cgatccgccg gcccacaagg tcctgatgat
      301 cgacagatcg tcctcgaaca tcggcacgcg tcgacctcgg gggacgccaa acggctcgcg
      361 atccgcaggg tggggtgaac cgaggccgaa ggccggtaac agacgtccgc cgctcaacac
      421 cgacagcgag gcgagttgtt tcgccatgag gtacgggtcg cgtccgggca gtacccagac
      481 gttcgcaccc aacttgatcc ggctggtccg accggcggcg atcgccagcg agacggtcgg
      541 gtcgagactt tcgctgacga cacgatccga gacccacaac gagttgaagc cgaggtcttc
      601 gacgaaatcc acgacctcgc cgaagtgcct cgggagcgca acctactgag cgcagcgggc
      661 gagggcgcac tgaaggcgct cgatgagttc gtcggagatg aacagtgcgc agtcgtcgag
      721 caactcctcg acgtcgcggt agcacccgag cgtccatcgg gcgaacatcg ccgtgtccat
      781 ccacgcggct tcgcacgaac gcagcccgct gcagggttcg gcgtcggacg cgacgaggtg
      841 gcgcaaccgt tcccagtcga gttcgccgca gagtcgtgcg atgacatcga ccgtcacgtc
      901 gagttcgggg tcgatcgcct cttcggcggc cagcccaaca tgggagagac cggtgaagtc
      961 gacaaacggg tgcatggcga caacatctgc ccacgaacgc acgtagaccg cggtggctgg
     1021 gtcggcgacc gcgtcgagaa ccgcggtgag ccatgccgca gcgacggcct cggcggggga
     1081 tcggtcctcg ggcatggtgg gcaggccgag cgtccgccgc agtagatcgc tgaggaatcc
     1141 gtcgaaggaa tcgccgctga tgattcgggc gtcgtggttt gtgcgaaccg cgactgcggc
     1201 acaaccacag ctgctttgaa cgagcgccga gtagccctgc tcaccgctca ctcgacattg
     1261 cgcgaccaac gccacagcgt gagcgccgcg caggtcgagg cccaacagat cctcggcagc
     1321 gtcgaagccg cgcaccaggg tcggagccga tgtcgggtcg gggtcgtcga tcgcccagag
     1381 tgcgttggtg ggctcatccg cgctgatcag ccgatgggcg agctcgcgac aggcgcgaag
     1441 cgacaagggg gaggaggtgg tgacggtcat ggcgtttcct tcgtgtgcgt gcacccgacc
     1501 ggttgcgcac tgcgtgcgcc gaggcgcgaa gcgagccgaa gccgggtgag tcgatactga
     1561 gcgggcatga ggcgtccgcg acctggccat tactcaatca cggaccaccg acactgaagc
     1621 gggcagcatc gagtccggac gcgacgaggc gccgcacccg aaggtgcggc gcctcgaaat
     1681 cacgagccga aacggctgct ggtaaacccg ttcgtcagtt cgaacaggtc gtcccgtcga
     1741 gggatgcgca cagcaggccg ctggcgccag cattgccggc ctggcc
//
LOCUS       DAOOMA010000593         1786 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_84603_length_1786_cov_2.852686, whole genome shotgun sequence.
ACCESSION   DAOOMA010000593 DAOOMA010000000
VERSION     DAOOMA010000593.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 1786)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1786)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1786
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_84603_length_1786_cov_2.852686"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..559
                     /locus_tag="PLP95_12895"
     CDS             <1..559
                     /locus_tag="PLP95_12895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB46746.1"
                     /translation="GFRVGGVGSLIRTAKARTAAELWSEPATAGIAAMEFFVRSEVVD
                     RLASTAPASTTPANGSAFTTNRSAEFYRWRYSFEPLRYRVWPFGNGGIVFRVRRRGAA
                     FEATICDVIEPDVPNAKLLREFGRLRKATGADYLLGTQASVRRSWGFAPLPGSGPMLT
                     WRPLAQPGVPTLAEVDLALGDLELF"
     gene            568..1701
                     /locus_tag="PLP95_12900"
     CDS             568..1701
                     /locus_tag="PLP95_12900"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HPB46747.1"
                     /translation="MLWLAKGLGPGGMERLLETHARVGDRSIFEFEAAYLVERPHSIV
                     GSLEELRVPVHRLEGGLASWPRQLRRLVAEHRIDVVHTHSPMVAALARPVLRSMRNRP
                     ALVYTEHNSWDCYGTATRLANAATYRLDDEQLAVSAAAATSAPAVLRPRMRELTHGID
                     VKRLQQLDIDRVEIRERLGIGAEAKLVITVAHLRAEKGYEVWLEAAKRVTEAREDVVF
                     VSVGHGPLEAELREQHRRLGLDDRVRFLGFRDDAVELLAAADVCCFASHSEGLPVVVM
                     EAAALGVPIVATAVGGLVGAIDDGVNGRLVPPGDPQALADALSEVLGDDDVRKQMGEA
                     MRSRAGRYDAIGAIGVIEALYREVHAAHRGAGLAEAHRRGGAV"
BASE COUNT          262 a          519 c          669 g          336 t
ORIGIN      
        1 ggggtttcgt gtcggcgggg tcggttcgct cattcgaacc gcgaaggctc gcacggcggc
       61 ggagttgtgg tcggaaccgg ccacggccgg gatcgctgca atggaatttt tcgttcgttc
      121 agaggtggtc gaccgactgg cgtccacagc gccggcgtcc accacgccag cgaacgggtc
      181 agcgttcacc accaaccgct ccgcggagtt ctaccgttgg cgttactcgt tcgagccgct
      241 gcgctaccgg gtgtggccgt tcggcaacgg cggcatcgta tttcgggttc gtcgacgggg
      301 tgcggcgttt gaggcaacga tctgtgacgt catcgaaccc gacgtgccaa acgcaaagct
      361 cctgcgcgaa tttggccggc tccgcaaggc gaccggtgcc gactacttgt taggaaccca
      421 ggcgtcggtg cgtcgttcgt gggggttcgc cccgttgccg ggttcggggc caatgctgac
      481 ctggcggccc ctcgcccagc cgggtgtacc gaccctcgcc gaggttgatc tcgcgctcgg
      541 cgacctggag ttgttctagt gatccgcgtg ttgtggttgg caaaggggct cggaccgggt
      601 gggatggagc gtttgctgga aacccatgcc cgcgtgggcg accgttccat cttcgagttc
      661 gaggcggcct atctggtgga gcgacctcac tcgattgtcg ggtcgttgga ggagttgagg
      721 gttccggttc accgactcga gggcgggttg gcgagttggc cgaggcagct gcgccgtctg
      781 gttgcggagc atcgcatcga tgtcgtccac acgcattcgc cgatggtggc ggcgctcgcc
      841 cgtccggtgt tgcggtcgat gcgcaatcga ccggcgctcg tctataccga acacaactcg
      901 tgggactgct acggcaccgc gacgcgtctg gcgaacgcgg cgacctatcg gctcgacgac
      961 gagcagcttg cggtgtccgc cgcggctgca acatcggcgc ctgcggtact gcggccgcgg
     1021 atgcgggagt tgacacatgg gatcgatgtc aaacgtttgc aacagctcga catcgaccga
     1081 gtcgagatcc gggagcggct cggcattggc gccgaggcga agttggtgat caccgttgcg
     1141 caccttcggg ccgagaaggg ctacgaggtg tggttggagg ccgccaagcg ggtgaccgag
     1201 gcacgcgagg atgtcgtgtt cgtcagcgtt gggcacggtc cgctggaggc ggaactccgc
     1261 gagcaacatc ggcggctcgg ccttgacgat cgggtgaggt tcctcggctt tcgggacgac
     1321 gccgtcgagt tgttggcagc ggccgacgtg tgctgtttcg cgtcccattc ggaggggttg
     1381 ccggtcgtcg tgatggaggc agccgcgctc ggggtgccga ttgtggccac cgcggtcggc
     1441 gggttggtcg gagcgatcga cgacggcgtg aacggacggc tggttccacc cggggacccg
     1501 caggcgttgg ccgatgcgct gagcgaggtg ttgggagacg acgacgtacg aaagcagatg
     1561 ggcgaagcga tgcggtcgcg ggctgggcgt tacgacgcca tcggtgcgat tggcgtgatc
     1621 gaggcgttgt accgcgaggt gcatgcagcg caccgtgggg ccggactcgc cgaggcgcac
     1681 cgacgcggag gtgccgtctg atgtgcggca tcgcaggcat ctacgatcct cgagccgccc
     1741 tgggcgcgga agcgttcgag gcgctgctcg tgcggatgac cgacac
//
LOCUS       DAOOMA010000594         1784 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_84710_length_1784_cov_3.864662, whole genome shotgun sequence.
ACCESSION   DAOOMA010000594 DAOOMA010000000
VERSION     DAOOMA010000594.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 1784)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1784)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1784
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_84710_length_1784_cov_3.864662"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(7..1062)
                     /locus_tag="PLP95_12905"
     CDS             complement(7..1062)
                     /locus_tag="PLP95_12905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020378785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-eliminating lyase-related protein"
                     /protein_id="HPB46748.1"
                     /translation="MMIDLPAPEHSFASDNAAGASPEVLEALAAANIGSALAYGDDRW
                     TRAAIDDVRRTLDAPAEVLFTWGGTGANVVGLGTLLQPWQAVICADSAHIVVDECGAP
                     VRFTNSQLIALATPDGKLRPDDLVAPSHWLGSEHHPQPRVVSITQSTELGTLYTVDEI
                     AALCDAAHALDMVVHLDGARIANAAAALNTDLRAMIRDTGVDAFTFGFTKNGAVFGEA
                     VCFVDPSLAASAAFVRKQAGQLVSKSRFVAAQISALLADDLWLRNARHANDRAQDLAQ
                     RVSAIDGVEVCGTAEVNAVFVRLPRPVLEVLREWSFFWEWDAGSDLVRWMTSFATTAE
                     DIDRFVDGIRVALRLPS"
     gene            complement(1114..>1784)
                     /locus_tag="PLP95_12910"
     CDS             complement(1114..>1784)
                     /locus_tag="PLP95_12910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB46749.1"
                     /translation="PVTLEGLPLSLDVFAVATRAVVVLIGTDTPARQIAKTAIGLSIE
                     SEQTAAIDVVVPRAIDLVLQRAKELIAAGLAAQVVDRCEERAELWRRLSMRSGLEPAA
                     IVLSLAPFPLPTIASMLRVSLVDLQTVVASWQRGDDPIDGTPVTELAPAIITSVVRPS
                     VAPLELPAGQTANPAGDPVGSGVADTNLAEESALSADHGALDEFLALPDGRGSRIRRL
                     LRRA"
BASE COUNT          289 a          583 c          625 g          287 t
ORIGIN      
        1 ggcgaatcat gacggcagtc ttagtgcgac tcgaatcccg tcgacgaacc ggtcgatgtc
       61 ctcggcggtc gtcgcgaaac tcgtcatcca ccgaaccagg tcgctgccgg cgtcccactc
      121 ccagaagaac gaccactcgc gcaagacctc aagaaccggg cgggggagcc ggacgaatac
      181 cgcgttcact tcggcggtgc cgcacacctc gaccccgtcg atggcgctca cacgttgcgc
      241 cagatcctgg gcgcggtcgt tggcgtggcg ggcgttgcgc agccacaggt catcggcgag
      301 gagcgcgctg atctgtgccg caacgaagcg gctcttcgac acgagttgtc ccgcttgctt
      361 tcgaacgaac gcggccgacg cggccagcga cgggtcgaca aagcacacgg cctcgccgaa
      421 gacggcgccg ttcttggtga agccgaaggt gaacgcgtcg accccggtat cgcggatcat
      481 ggccctcaga tcggtgttga gcgcggcggc ggcgtttgcg atgcgtgcgc catcgaggtg
      541 gaccaccatg tcgagcgcat gagccgcatc gcacagggct gcgatctcgt cgaccgtgta
      601 cagcgtgccc agttcggtcg attgcgtgat cgacacgaca cgaggctgcg ggtgatgttc
      661 gctgcccagc caatgtgatg gggccaccag gtcgtcgggg cgaagctttc cgtcgggcgt
      721 cgccagcgcg atcagttgtg agttggtgaa acggaccggc gcgccgcact cgtcgacgac
      781 gatgtgagcg ctgtcggcac agatgacggc ttgccagggt tgcaacaggg tgccgagacc
      841 gacgacgttg gctcccgtgc cgccccaggt gaacagcacc tccgctggag cgtcgagtgt
      901 tcgccggacg tcgtcgatcg cggcgcgggt ccagcggtcg tcgccgtagg ccaacgccga
      961 gccgatgttc gctgcggcga gcgcctcgag gacctccggg gatgcgccgg cagcgttgtc
     1021 gctcgcaaag gagtgttccg gtgctggtag atcgatcatc atccgatcat cggtctgcgg
     1081 gggtcgacgg cgccactacc gacgctgtcg gcgtcacgcc cggcgcaaca gtcggcgaat
     1141 cctcgagccg cgcccgtcgg gcagtgccag gaattcatcg agcgcaccgt ggtctgcgct
     1201 gagcgcggac tcctcggcga ggttcgtgtc ggcgacgccc gacccgacgg gatcgcccgc
     1261 cggattcgcc gtttggccgg cgggcagctc gagcggagcg acgctcgggc gcacgaccga
     1321 cgtgatgatc gctggcgcca actcggtgac cggggtgccg tcgatcgggt cgtcgccacg
     1381 ctgccatgag gcgacgaccg tttgaaggtc gaccagcgac acccgcagca tcgacgcgat
     1441 cgtcggcagg gggaacgggg cgaggctcag cacgatcgcc gctggctcga ggccggatcg
     1501 catcgacagg cggcgccaca gttcggctcg ttcctcgcaa cggtcgacga cctgtgccgc
     1561 caggcccgcg gcgatgagtt ccttcgctcg ctgcaacacg agatcgatcg cccgcggcac
     1621 gacgacgtcg atcgccgcgg tctgttcgga ttcgatcgac agtccgatcg cggtcttggc
     1681 gatctggcgc gccggggtgt cggtgccgat cagtacgacc accgcccggg ttgcgactgc
     1741 gaacacgtcg aggctcaacg ggaggccttc gagcgttacg ggca
//
LOCUS       DAOOMA010000595         1779 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_85055_length_1779_cov_4.845708, whole genome shotgun sequence.
ACCESSION   DAOOMA010000595 DAOOMA010000000
VERSION     DAOOMA010000595.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 1779)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1779)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1779
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_85055_length_1779_cov_4.845708"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            48..1172
                     /gene="steA"
                     /locus_tag="PLP95_12915"
     CDS             48..1172
                     /gene="steA"
                     /locus_tag="PLP95_12915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012226778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative cytokinetic ring protein SteA"
                     /protein_id="HPB46750.1"
                     /translation="MTQPITGAAFADARTKDLVKRLLPGQIAVIDHRDLDRVAAETLV
                     ESGIAAVINADESSSGRYPNEGPIIVLEAGIALVDRVGAQIMDAVSDGLEVTIDPDGR
                     ISADGETIGHGVRQSLESIHEIHEESRKNLGQEFIRFIENTVEYFDHNQDLVSDDLEI
                     PDVGIDFEGRHVLMVVRGHDYREDLTLLRNSGYVAEQRPLLIGVDGGADALLELGMTP
                     DVIVGDFDSVTEQALRSGAKLVVHAFPDGRAPGAERLDELGCDYAVFKASGTSEDIAM
                     LMAYNQGAELIVAVGTHSSMVDFLDKGRSGMASTILTRMKVGPALVDAKGVSRLYHSG
                     VRKRDLLLLVVGALIAIAIIIVVSDPVRLVIHSIWSDLTG"
     gene            1181..>1779
                     /locus_tag="PLP95_12920"
     CDS             1181..>1779
                     /locus_tag="PLP95_12920"
                     /inference="COORDINATES: protein motif:HMM:NF022824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055070 - copper ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper transporter"
                     /protein_id="HPB46751.1"
                     /translation="MINLRFHIVSIVAVFLALAIGILTGSTLLDRATIQVLEDRQQSL
                     DDRNEELRGRVAALEDNTKRRDLAQAALDDQALAELVPGLVADEPLLLIAARGIDEDT
                     VNGTSELLADAGANVLGTAWFDERLDLREAQVRTRLAAALNVSDSTLAALVEALAVPL
                     SVPTPATTTTTAASVDPAAPVDPAAPVDPAAPVDPAAPV"
BASE COUNT          290 a          582 c          605 g          302 t
ORIGIN      
        1 catgcggccg aactgctgag ctcggcaggc cgcggctcgg gggcgcaatg acccagccga
       61 tcaccggcgc ggccttcgcc gacgctcgaa ccaaggacct cgtcaagcgg ctgctgcccg
      121 gacagatcgc ggtgatcgac caccgcgacc tcgatcgggt ggcggccgag acgctggtcg
      181 agtcggggat cgccgcggtc atcaacgcgg acgagtcgag ttctggccgc tacccgaacg
      241 aggggccgat catcgtgttg gaggcgggga tcgccctcgt cgaccgggtc ggcgcgcaga
      301 tcatggacgc cgtgtccgac gggctcgagg tgaccatcga tcccgatggc cgtatcagcg
      361 cggacggcga gacgatcggc catggggtgc gccagagcct cgagtcgatt cacgagatcc
      421 acgaggagtc gcgcaagaac ctgggccagg agttcatccg cttcatcgaa aacaccgtcg
      481 agtatttcga tcacaaccag gacctggtga gcgacgatct cgagatcccc gatgtcggca
      541 tcgactttga gggccgtcac gtgctgatgg tggtgcgcgg tcacgattac cgcgaagacc
      601 tgaccctgct gcgcaacagc ggatatgtcg ccgagcaacg tccgttgctc atcggcgtgg
      661 acggcggagc ggacgcgttg ttggaactcg gcatgacccc cgatgtgatc gtgggggatt
      721 tcgattcggt gaccgagcag gcgctgcgca gcggcgcgaa gctcgtcgtt cacgccttcc
      781 ccgacgggcg ggcgccgggg gcggaacgtc tcgacgaact cggctgcgac tacgcggtgt
      841 tcaaggcctc ggggacgagc gaggacatcg cgatgttgat ggcctacaac cagggcgccg
      901 agctgatcgt cgccgtcggg acccacagtt cgatggtcga ctttctcgac aagggtcgct
      961 ccggcatggc ctcgacgatc ctgacccgga tgaaggtcgg ccccgcactc gtcgacgcca
     1021 agggggtgag ccgcctctac cactcggggg tgcgcaagcg cgacctgctc ctgttggtcg
     1081 tcggcgcgct catcgccatc gccatcatca tcgtcgtatc ggatccggtg cgactcgtga
     1141 tccactcgat ctggtcggat ctgacgggct gaggagccac atgatcaacc ttcgtttcca
     1201 catcgtgagc atcgttgcgg tcttcttggc gctcgccatc ggcatcttga ccggctccac
     1261 cctgctcgac cgtgcgacca ttcaggtgct cgaggatcgc cagcagagcc tcgatgaccg
     1321 caacgaggaa ctgcgcggcc gggttgcggc gctcgaggac aacaccaagc gccgcgacct
     1381 cgcccaggcg gccctcgacg atcaggcgtt ggccgagttg gttcccggtc tggtcgccga
     1441 cgagccgttg ctgttgatcg ccgctcgggg gatcgacgag gacacggtga acggcacctc
     1501 ggaattgttg gccgacgccg gtgcgaacgt gttgggcacc gcctggttcg acgaacgcct
     1561 cgaccttcgc gaggcccagg tgcgtacccg gctcgcagcg gcgttgaacg tgtcggattc
     1621 gacgttggcg gcgctggtgg aggccctggc ggttccgctg agcgttccga cgcctgccac
     1681 gaccacgacg accgccgcat cggtggaccc tgcggcaccg gtcgatcccg ccgcaccggt
     1741 cgaccctgcg gcaccggtcg atcccgccgc tccggtcga
//
LOCUS       DAOOMA010000596         1775 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_85336_length_1775_cov_4.816279, whole genome shotgun sequence.
ACCESSION   DAOOMA010000596 DAOOMA010000000
VERSION     DAOOMA010000596.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 1775)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1775)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1775
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_85336_length_1775_cov_4.816279"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..188
                     /locus_tag="PLP95_12925"
     CDS             <1..188
                     /locus_tag="PLP95_12925"
                     /inference="COORDINATES: protein motif:HMM:NF012339.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HPB46752.1"
                     /translation="DSIEAAGQLAPSGCRNGICHTCSTEVIDGCALNLRDGRIVEAGQ
                     HVQLCISAPLTDLTLAL"
     gene            255..1424
                     /locus_tag="PLP95_12930"
     CDS             255..1424
                     /locus_tag="PLP95_12930"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: protein motif:HMM:NF012699.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016717 - oxidoreductase activity, acting
                     on paired donors, with oxidation of a pair of donors
                     resulting in the reduction of molecular oxygen to two
                     molecules of water [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA desaturase"
                     /protein_id="HPB46753.1"
                     /translation="MTIVAPDLDQLTSHEPVGLATHTVEPAAQHPGIAPYGDTELSEK
                     QLGELAAELDALRDELLADIGQRDADYIRRIVKVQKSLELGGRASLMAGVLPPMWVVG
                     VAMLAASKILENMEIGHNVMHGQYDWMRDPHLHSSTYEWDNVCPSSQWKHYHNAVHHQ
                     WTNVRGLDDDLGYGVFRVDPTRKWSPAHAAQPLIFVALAALFQYGVGYQDSYAGRPEG
                     QRADWAWRKPKLMETARKIRRQWLKDYVAFPALAAPLGAPSAVAVLSGNAAANLIRNV
                     WSFSVIFCGHFPDGVAIFDKADAIDESRGHWYLRQITGSANFTGGRLMDIMSGNLNHQ
                     VEHHVFPDIPANRYAEVAPKLREICGRYGLRYNTGSMPKQFATVVKKICRLALPG"
     gene            1520..>1775
                     /locus_tag="PLP95_12935"
     CDS             1520..>1775
                     /locus_tag="PLP95_12935"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygenase MpaB family protein"
                     /protein_id="HPB46754.1"
                     /translation="MFQPEPRSIGPNSLLWRYAGDQRLGFVGLATGILQLMHPGIGAG
                     VAQHSAFFTEPWDRIQRSMPLILGVIYNEDAENTGNSVREF"
BASE COUNT          338 a          639 c          509 g          289 t
ORIGIN      
        1 tcgacagcat cgaggcagcg gggcagctcg caccctcggg gtgtcgaaac ggcatctgcc
       61 acacgtgttc gaccgaggtg atcgacggct gcgcgctcaa cctgcgcgac ggacgaatcg
      121 tcgaggccgg ccagcacgtt cagttgtgca tctccgctcc gctcaccgac ctcacgctcg
      181 cgctgtaacc cagtgcccat ccccaccgcc acccatcgcc acaccgccgc cgcacagcac
      241 aggacgttcc cacgatgacc atcgtcgccc ccgacctcga ccagctcacg tcgcacgagc
      301 cggtcggcct cgccacccac accgtcgaac ccgccgccca acacccgggc atcgccccct
      361 acggcgacac cgagctgagc gagaagcaac tcggtgagct cgccgctgaa ctcgacgcgt
      421 tgcgcgacga attgctcgcc gatatcggcc aacgagacgc cgactacatc cgtcgaatcg
      481 tcaaggtgca aaagtccctc gaactcggtg ggcgcgcctc gctgatggcg ggagtgctgc
      541 cgccgatgtg ggtggtcggt gtcgcgatgc tcgcggcgtc gaagatcctc gagaacatgg
      601 aaatcggcca caacgtcatg cacggccagt acgactggat gcgagacccg cacctgcact
      661 caagcaccta cgagtgggac aacgtgtgcc cttcctcgca gtggaagcac taccacaacg
      721 ccgttcacca ccaatggacc aacgtgcgcg gactcgacga cgacctgggc tacggcgtgt
      781 ttcgcgtcga ccccacacga aagtggagcc ccgctcacgc cgcccagccg ctgatcttcg
      841 tcgcgctcgc ggcgctgttc cagtacggcg tcggatacca ggactcctat gccgggcgtc
      901 ccgagggcca acgcgccgac tgggcgtggc gcaagccgaa actcatggag accgcccgca
      961 agattcgccg tcagtggctt aaggactacg tggcgtttcc tgccctggcc gcaccactcg
     1021 gagcaccatc ggcggtggcc gtgctcagtg gcaacgcagc cgcgaacctc atccgcaatg
     1081 tgtggtcctt cagcgtgatc ttctgtggcc atttccccga tggcgtcgcc atcttcgata
     1141 aggccgacgc catcgacgaa agtcgcggcc attggtatct gcgccagatc accggctcgg
     1201 ccaacttcac cggcgggcgg ctcatggaca tcatgagcgg caacctcaac catcaggtcg
     1261 aacaccacgt gttccccgac atcccggcga atcgctacgc cgaggtcgcc ccgaaactcc
     1321 gcgagatctg cggccgctat ggcctgcgct acaacacggg gtcgatgccg aagcagttcg
     1381 caacggtggt caaaaagatc tgtcgcctcg ccctaccggg gtagcgcctc gcggcaccgc
     1441 cgcaaccgac ctgcccccgg agggttgaat tcgacgccca ccgttgacag cccggaccga
     1501 tggaacaatt attgttctta tgttccagcc tgaaccccgg tcgatcggac caaactcgct
     1561 cctgtggcgc tatgcgggcg accaacgact cggattcgtc ggcctcgcca ccggcatctt
     1621 gcagctcatg caccccggca tcggtgcggg agtcgcccag cactcggcgt tcttcaccga
     1681 gccgtgggac cgaattcaac gatcgatgcc gctcatcctc ggcgtcatct ataacgagga
     1741 tgcggagaac accggcaact ccgtgcgcga gtttc
//
LOCUS       DAOOMA010000597         1774 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_85406_length_1774_cov_4.686446, whole genome shotgun sequence.
ACCESSION   DAOOMA010000597 DAOOMA010000000
VERSION     DAOOMA010000597.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 1774)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1774)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1774
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_85406_length_1774_cov_4.686446"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1774
                     /locus_tag="PLP95_12940"
     CDS             <1..>1774
                     /locus_tag="PLP95_12940"
                     /inference="COORDINATES: protein motif:HMM:NF013727.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA translocase FtsK 4TM domain-containing
                     protein"
                     /protein_id="HPB46755.1"
                     /translation="RKSTRSTRSTGSTRSSGRTAAAPSRGQRVAEGVSLAFDGHGHDM
                     WGLACIALAIVSAIGIYAGSAGIVGSAVATAIGSVVGVAKFIAPPAIAALGIWLIRGP
                     IDAEELAAADPDLVVAHTRTAARRAIGGVILVLSILGLIHIAVDPPPLSTSPDAFKDA
                     GGYVGAIGGDGLVRLIGTPGAIAVLALLVILGLTLCIGLPFRDLFPILGRTLSPAVAG
                     GSRSFSNLFRIGAEPSDVELFDYEADFDDEDEPQPKAKPRKKAAAKAGADGAGGTAVQ
                     PKPQEPTIVLPTVNAGTTVEQLEIELSPLRPDSIWKLPALSLLKRTSAKPPDTGPIQK
                     RGIQLEAALAEHGVTTRLSGMVVGPTITRYELELGQGVKVNQVVNLSKDIAYAMASPD
                     VRIIAPIPGKHAVGVEVPNQKRELVRVGDILCSQEAQKAKTPLAVAVGRDIEGNSVLV
                     DLAKMPHILIAGQTGAGKSSCINSIMTSVLMRATPDDVRLILVDPKRVELTQYNRVPH
                     LLTQVVTNPKKAANALAWAVAEMERRYELLEEVGSRDLASYNERFDRGELQPGLGEVD
                     KEYQRLPLILIVVDELADLMMVAAR"
BASE COUNT          294 a          559 c          616 g          305 t
ORIGIN      
        1 gcgcaagtcg acgcgctcga cgcgctcgac cggtagcacg cgttcctcgg ggcgcaccgc
       61 cgccgcgccg agccgcgggc agcgggtcgc cgagggcgtg tcgttggcct tcgacggtca
      121 tggccacgac atgtgggggc tggcgtgcat cgcgctggcc atcgtgagcg cgatcggcat
      181 ctacgcgggt tctgccggca tcgtcgggtc cgcggtggcg acggcgatcg gctcggttgt
      241 cggggtggcg aagttcatcg ctccgcccgc catcgcagcg ctgggcatct ggcttatccg
      301 cgggccgatc gacgccgagg agttggcggc cgccgacccg gatctcgtgg tcgcgcacac
      361 cagaaccgcg gcgcgtcggg ccatcggcgg cgtcatcttg gtgttgtcga ttctcggcct
      421 catccatatt gcggtcgatc caccgccgtt gtcgacgagt cccgacgcgt tcaaggacgc
      481 cggcggatac gtgggagcga tcgggggcga cggcctcgtg cgcctgatcg gcaccccggg
      541 tgcgattgca gtgctggcgt tgttggtgat tctggggctg acgttgtgca tcggattgcc
      601 cttccgcgac ctgtttccga tactcgggcg cacgttgagc cccgcggtcg ccggggggag
      661 ccgctcgttc agcaacctgt ttcgcatcgg tgccgagccc tccgacgtcg agctgttcga
      721 ttacgaggcc gacttcgacg acgaggacga accgcagccc aaagcgaagc cgcgaaagaa
      781 ggccgcggcc aaggcggggg ccgacggtgc cggtggcacg gcggtacagc ccaaaccgca
      841 ggaaccgacg atcgtgttgc ccaccgtcaa cgccggcacc acggtggaac aactcgagat
      901 cgaactgtcg ccgttgcgtc ccgattccat ttggaagctt ccggcgttgt cgctgctgaa
      961 gcgcacgagt gcgaagccgc ccgacacggg cccgattcaa aaacggggca tccaactcga
     1021 ggcggcgttg gcagagcacg gcgtcacgac acgactgtcg ggcatggtgg tcggcccgac
     1081 gattacccgc tacgagctcg aactgggcca gggcgtgaag gtgaatcagg tcgtcaacct
     1141 gtccaaggac atcgcgtatg cgatggcgag ccccgatgtg cgcatcatcg ctccgattcc
     1201 cggcaagcat gccgtcggtg ttgaggttcc caatcagaaa cgcgagctgg tgcgggtcgg
     1261 cgacatcttg tgctcccagg aggcgcagaa ggcgaagact ccgctggcgg ttgcggtcgg
     1321 tcgcgacatc gagggcaatt cggtgttggt cgacctggcg aagatgcccc acatcctgat
     1381 cgccggtcag acgggtgccg gcaagtcgag ttgcatcaac tcgatcatga cctcggtgct
     1441 catgcgcgcc acccccgacg acgttcgcct gatcctggtc gacccgaagc gggtcgaact
     1501 cacccagtac aaccgggtgc cgcacctgtt gacccaggtg gtgaccaatc cgaagaaggc
     1561 cgccaacgcg ctggcgtggg cggtggccga gatggagcgg cgttacgagc tgctcgaaga
     1621 ggtcggctcc agagacctgg cgagctacaa cgagcgcttc gatcgcggcg agcttcaacc
     1681 tggtctgggt gaggtcgaca aggagtatca acgcctaccc ctcatcttga tcgtggtcga
     1741 cgagttggcc gacctgatga tggtggcggc gcgc
//
LOCUS       DAOOMA010000598         1767 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_85965_length_1767_cov_2.970794, whole genome shotgun sequence.
ACCESSION   DAOOMA010000598 DAOOMA010000000
VERSION     DAOOMA010000598.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 1767)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1767)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1767
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_85965_length_1767_cov_2.970794"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..246
                     /locus_tag="PLP95_12945"
     CDS             <1..246
                     /locus_tag="PLP95_12945"
                     /inference="COORDINATES: protein motif:HMM:NF024966.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HPB46756.1"
                     /translation="AFPAFQHLVSNPMLHIDGDTATGRTMCFNPMAVNAASPGDHNVF
                     FCGLWYVDTFVRTERGWRIASRTEDKSFTYNMSGAFR"
     gene            243..1139
                     /locus_tag="PLP95_12950"
     CDS             243..1139
                     /locus_tag="PLP95_12950"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03619 family F420-dependent LLM class
                     oxidoreductase"
                     /protein_id="HPB46757.1"
                     /translation="MRFSYAESLCDVTHYVSLAQAAEEAGWHSMVVPDSVAYPRDSDS
                     KYPYNGTGEREFLDDQPFIEPFVLIPTLAAVTERIKFATFVLKLAIRQPVIVAKQATS
                     TAVLSNNRLTLGVGLSPWPEDFTITQSQWRARGRRMDEMIDIVSGLCRPSADEGSPST
                     DFFEYSGSFYEIPAIKMCPAPTRKIPILIGGQSDAALKRAARVGDGWMHAGMGDGSDL
                     GEQIATLSRYRTEFGRCDEPFEIHVISLDAYSVDGVKRLEDLGVTDVIVGFRDPYSMP
                     DTPLQPKLDALRRFADQVIAKV"
     gene            complement(1166..>1767)
                     /locus_tag="PLP95_12955"
     CDS             complement(1166..>1767)
                     /locus_tag="PLP95_12955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB46758.1"
                     /translation="LPATLLIDAYRSDTAAPIPPTTFLVTPTDTGVSLSWSATNDDDS
                     GLSGYLIHRDDVYLGYVPADTTTWFDPHVVDGPTTYHVRGIDRAGNYAGPASRTIGGG
                     DDVAPPAPANVQLTYGAETATGYEVTLSWDGVEDLPAVGASGLSGYWLNIDGAEKWLP
                     ADTTSYTVDTERVSSAFVHAMDRNNNFSERARPAVPTHP"
BASE COUNT          313 a          585 c          549 g          320 t
ORIGIN      
        1 gcattcccgg cgtttcagca cctggtgtcg aacccgatgc tgcacatcga tggcgacacc
       61 gccaccgggc gaacgatgtg tttcaacccg atggccgtca acgccgcctc acccggagac
      121 cacaacgtgt tcttctgtgg gctctggtac gtcgatacct tcgttcgcac cgagcgcgga
      181 tggaggatcg cctcgcgcac cgaagacaag agcttcacct acaacatgtc cggagcattt
      241 cgatgagatt cagttacgcc gagtcgctgt gcgacgtcac ccactacgtc tcactcgctc
      301 aggccgccga ggaggccgga tggcattcga tggtcgtgcc cgactcggtc gcctaccccc
      361 gcgactccga ctcgaagtac ccctacaacg gcaccggcga acgcgagttt ctcgacgacc
      421 agcccttcat cgagccgttc gtcctgatcc cgacactcgc tgcggtgacc gagcgcatca
      481 agttcgcgac cttcgtgttg aagttggcca tccgccaacc ggtgatcgtc gccaagcagg
      541 ccacctccac cgcggtgttg tcgaacaacc gcctcacgct cggggtcggg ctgtcgccgt
      601 ggccagagga tttcacgatc acgcagtcgc aatggagggc ccgcggcaga cgcatggacg
      661 agatgatcga catcgtctcc ggactgtgcc ggccctctgc cgacgaaggg tcacccagca
      721 ccgacttctt cgagtactcc ggttcgttct atgaaatccc cgccatcaag atgtgtccgg
      781 cgccgacccg caagatccca atcctaatcg gtggccaaag cgacgccgcg ttgaagcggg
      841 cggcgcgcgt gggcgacggc tggatgcacg caggaatggg tgacggctcc gacctcggcg
      901 agcagatcgc gaccctgtct cggtatcgca ccgagttcgg gcgctgcgac gagcccttcg
      961 agattcacgt catcagcctc gacgcctact ccgtcgacgg ggtgaaacgt cttgaggatc
     1021 tcggggtcac cgatgtgatc gtcggcttcc gcgaccccta ttcgatgccc gacactcccc
     1081 ttcaaccgaa actcgacgcc ttgcgacgct tcgccgacca ggtcatcgcc aaggtttgag
     1141 cgcgtccggc cggcgcagcg cgcgtctacg ggtgtgtggg caccgcgggt cgtgcacgtt
     1201 cggagaagtt gttgttgcga tccatcgcat gcacaaaggc gctggacacg cgctcggtat
     1261 cgacggtgta ggaggtcgtg tcggcgggca gccacttctc cgctccatcg atgttcagcc
     1321 agtaacccga caggccggac gctccgaccg caggaaggtc ctcgacccca tcccaactga
     1381 gcgtcacctc gtagccggtt gcggtctcgg cgccgtaggt cagctggacg tttgccggtg
     1441 ccggaggggc gacgtcgtct cccccaccga tcgtgcgcga tgcgggcccg gcgtagttgc
     1501 cggcgcggtc gattccccgc acgtggtagg tggtcggccc gtcgacgacg tgtggatcga
     1561 accacgtggt cgtgtcggcc ggaacataac ccaggtacac atcgtcgcga tgaatcaggt
     1621 atcccgacag acccgagtcg tcgtcgttgg tcgccgacca cgacagcgat acgccggtgt
     1681 cggtcggggt gacgagaaac gtggttgggg gaatcggcgc agccgtgtcg gagcggtacg
     1741 catcgatgag caacgtcgca ggcaacc
//
LOCUS       DAOOMA010000599         1764 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_86178_length_1764_cov_5.780573, whole genome shotgun sequence.
ACCESSION   DAOOMA010000599 DAOOMA010000000
VERSION     DAOOMA010000599.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 1764)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1764)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1764
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_86178_length_1764_cov_5.780573"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            58..735
                     /locus_tag="PLP95_12960"
     CDS             58..735
                     /locus_tag="PLP95_12960"
                     /inference="COORDINATES: protein motif:HMM:NF039921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HPB46759.1"
                     /translation="MDHTDPGVNTSDDLRAADGRVPGRRGRATRERLIACTLNMLQET
                     SYRDLKVVDIAREAGTSPATFYQYFPDVESALVSLVEQMAEDGNHRLTRIVGEGDWRL
                     KYAYDTCAEIVAAFFAFWDDNAALMRVLDLTSAEGDTRFRSIRTKLLNELTVELADLI
                     GRFRDAGKHPADINPMAVAGILVSMLSHTAGHRNDFAVHGIAVPDTQRALERLLYSSI
                     CGAKPTR"
     gene            788..1174
                     /locus_tag="PLP95_12965"
     CDS             788..1174
                     /locus_tag="PLP95_12965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB46760.1"
                     /translation="MLDSQVVEQMNAAVAGTGEVGVPLAAWSVLMRACAEPNPLQLHF
                     DVKDALGYRHALITEHRVVAESAPAIGGRYSHQQILRTVGREQDRSIGRGRSWRIDVE
                     HLDAGGTVVAVESYEAFGYHPKAAVT"
     gene            1171..1500
                     /locus_tag="PLP95_12970"
     CDS             1171..1500
                     /locus_tag="PLP95_12970"
                     /inference="COORDINATES: protein motif:HMM:NF013722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC/PaaZ C-terminal domain-containing protein"
                     /protein_id="HPB46761.1"
                     /translation="MRIEVTEEFIRSGSSAFGVDVAVHHDRNAAQAAGLRHIIMSTPH
                     LSALMEAVVVSTFGGRVRDLRISMIRPVFAGTTVEIDVAQTHAGWAVTMTDVEQPDRA
                     LCAAACS"
     gene            complement(1550..>1764)
                     /locus_tag="PLP95_12975"
     CDS             complement(1550..>1764)
                     /locus_tag="PLP95_12975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HPB46762.1"
                     /translation="ARNMITELERTDDSDVPVYIPGNPVKMSKVADGPDTRIPWVGEH
                     TDSVLREELGIDSGRLAELRAEGVIN"
BASE COUNT          292 a          540 c          612 g          320 t
ORIGIN      
        1 gatgaccccc gagtcgctcg agcacgcgct cgacctcgtc ggctcgtacg gagagcaatg
       61 gatcacaccg acccaggggt gaacacgagc gacgacctac gcgccgccga cggtcgcgtg
      121 cccggccgtc gcggccgtgc gacccgcgag cggctcatcg cgtgcacgct caacatgttg
      181 caggagacga gctaccgcga cctcaaggtc gtcgacatcg cccgtgaggc tggcacgtct
      241 ccggcgacct tctatcagta cttccccgac gtcgagtccg cgttggtcag cctggtcgag
      301 cagatggccg aggacggcaa tcatcggctc acccgtatcg tcggtgaggg tgactggagg
      361 ttgaagtacg cgtacgacac gtgtgcggag atcgtcgcgg cgttcttcgc gttctgggac
      421 gacaacgccg cgctcatgcg ggtgctcgat ctcacctcgg ccgagggcga cacgcgattt
      481 cgctccatcc gcaccaaatt gctcaacgag ttgacggtgg aactcgcgga cctcatcggt
      541 cggttccgcg acgccggcaa gcatcccgcc gacatcaacc cgatggcggt ggccggaatt
      601 ctggtgtcga tgctgtcgca caccgcgggg caccgtaacg acttcgcagt gcacggcatc
      661 gcggtgcccg acacgcagcg agcgttggaa cggctcctgt attcgtcgat ctgtggtgcc
      721 aagccaacgc ggtgacgcgg tcgccgactg gatcggtcga cctcgatacc ggcaatcggc
      781 acaatgggtg ctggattcgc aggtggtcga gcagatgaac gctgcggtgg ccggaaccgg
      841 cgaggttggc gtaccgctgg cggcgtggtc ggtactcatg cgcgcgtgtg cggagcccaa
      901 tccactgcaa ctgcacttcg atgtgaagga cgcgctcggc tatcgccacg cgttgatcac
      961 cgagcatcgc gtcgtggcgg agtcggcgcc ggcgatcggc ggacggtact cccaccagca
     1021 gatcctgcgc acggtggggc gtgagcagga ccgctcgatc gggcgggggc gaagctggcg
     1081 catcgacgtc gaacatctcg atgccggggg tacggtcgtc gccgtcgaga gctatgaggc
     1141 ctttggctat cacccgaagg ctgctgtgac gtgaggatcg aggtcaccga ggaattcatc
     1201 cggagtggtt cgtcggcgtt cggcgtggac gttgcggttc accacgaccg aaatgccgcg
     1261 caggcggcgg gactgcgcca catcatcatg tcgacaccgc atctttcggc gctgatggag
     1321 gcagtggttg tgtccacgtt cgggggacga gttcgcgatc ttcggatctc gatgattcgc
     1381 cccgtgttcg ccgggacgac cgtcgagatc gatgtcgccc agacccatgc aggttgggcg
     1441 gtgacgatga ccgatgtgga acaacccgac cgggcgttgt gtgccgcggc ctgtagttga
     1501 ggtgtggctg cggtgtgccg ccgtggctgg aacgcagagg cggctgcgct cagttgatga
     1561 cgccctcggc acgaagctct gcgagacgcc cggagtcgat gcccaactcc tcgcggagga
     1621 ccgaatcggt gtgttcgccg acccagggga tgcgagtgtc gggtccatcc gcgaccttcg
     1681 acatcttgac cgggttcccg gggatgtaga ccggtacgtc gctgtcgtcg gtgcgctcga
     1741 gttcggtgat catgttgcgg gcga
//
LOCUS       DAOOMA010000600         1764 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_86195_length_1764_cov_3.441194, whole genome shotgun sequence.
ACCESSION   DAOOMA010000600 DAOOMA010000000
VERSION     DAOOMA010000600.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 1764)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1764)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1764
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_86195_length_1764_cov_3.441194"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..209
                     /locus_tag="PLP95_12980"
     CDS             <1..209
                     /locus_tag="PLP95_12980"
                     /inference="COORDINATES: protein motif:HMM:NF017984.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0036297 - interstrand cross-link repair
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="crosslink repair DNA glycosylase YcaQ family
                     protein"
                     /protein_id="HPB46763.1"
                     /translation="VRVKGADPIQQERMIGDYVDAYGSITRSQAAALCQLSPTQARAV
                     LKRMVDREELRLVGERRGAKYVRA"
     gene            complement(461..1402)
                     /locus_tag="PLP95_12985"
     CDS             complement(461..1402)
                     /locus_tag="PLP95_12985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020502092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HPB46764.1"
                     /translation="MRIGLIAIDGCFGSAIASIIDIVRVADGARGDVDPRIDPIELAI
                     LGPKRRVTTTASMTLSVDHPLSESAEFDVVVVPALGTLTAAATNDALQSRDARSVIAS
                     LGRLDDATTRIAAACTGVFAVAETGRMHHRRATTSWFLGPEFLKRYPTVALDLDTMVV
                     VDGNLVTAGAAFAHIDLALSLVRSISPDLAQHVAKLLIIDERPSQAAFVAYEHLRHED
                     PIVVEFERFVRARLDEPFNVAFVAQSLGTSRRTLERRVRAALNLTPLGFVQRLRIERA
                     RHLSATTDLTSAEIALRVGYANAETLRSLLRRERRRS"
     gene            1511..>1764
                     /locus_tag="PLP95_12990"
     CDS             1511..>1764
                     /locus_tag="PLP95_12990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009883809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HPB46765.1"
                     /translation="MSTPASLPYAFVAKIVAADGQHDALADLLAGAVALANEEVGTIV
                     WFAVRTHADTFWIFDAFPDEAARDAHANGAIVAALLANQHL"
BASE COUNT          328 a          539 c          596 g          301 t
ORIGIN      
        1 acgtgcgggt gaagggcgca gaccccatcc agcaagaacg aatgatcggt gactacgtgg
       61 atgcgtacgg gtcgatcacc cgatcgcaag cggccgcact gtgtcagttg tccccgaccc
      121 aggcaagggc cgtgctcaag cgcatggtcg atcgggagga gttgcgactg gttggtgaac
      181 gccgcggcgc caagtatgta cgcgcgtgaa cgggggtcgg tgtgcctgaa ggcacgcctc
      241 gagctgtgcc tgagttgcgg tcgaatgctg cacctgacca ggtcaccgac ttaagaaaac
      301 tacctacgtt tgggtagttt ccctgaacta cctgtttcgg tttcgcggtt ccggacctgg
      361 ggtgcccacg ccggaagatg tcaagcagtc tctcgacgcg acgtgctgag aggtggttcc
      421 agtcgagcac taggacaccg gctacaggca tggcgatagg tcaggaacgg cgtcgctccc
      481 tacgcaggag ggagcgcaga gtctcggcgt tcgcgtagcc gacccgtagc gcgatctcgg
      541 cggaggtgag gtccgtggtt gctgagaggt gccgagctcg ttcgatgcga agccgttgga
      601 cgaagccgag cggagtgagg ttgagcgccg cacggactcg tcgttcgagg gtgcgccggc
      661 tggtgccgag cgactgcgcg acgaaggcga cgttgaacgg ttcgtccagg cgggcgcgca
      721 cgaagcgttc gaactcgacg acgatcgggt cctcgtgccg gagatgttcg taggcgacga
      781 aggccgcctg cgacggacgc tcgtcgatga tgaggagctt ggcgacatgt tgggccaggt
      841 cggggctgat cgatcgcacg agtgagagcg cgaggtcgat gtgggcgaac gcggcgccgg
      901 cggtgacgag gttcccgtcg accacgacca tggtgtcgag atcgagggcg acggtcggat
      961 agcgcttcag gaactccggc cccaggaacc agctggtcgt cgcccgccga tgatgcatcc
     1021 gtccggtctc ggcgacagcg aacacgccgg tgcacgccgc ggcgatccgg gtggtcgcgt
     1081 cgtcgaggcg cccgagcgag gcgatgaccg aacgagcatc tcggctctgg agggcgtcgt
     1141 tggtagcggc ggccgtgagg gttccaagcg cagggacgac gaccacgtcg aactctgcgg
     1201 actccgacaa tgggtggtcc acagacaggg tcatcgatgc cgtcgtggtc actcgccgtt
     1261 tcggtccgag gatggcgagt tcgatcgggt cgatccgcgg gtcaacatcg ccgcgggctc
     1321 cgtcggccac ccgcacgatg tcgatgatcg acgcgatagc cgaaccgaag cagccgtcga
     1381 tcgcgatcag tccgatacgc atggcgcaaa caatagcaat actgccgtat acgccacttc
     1441 tcacatgccc ttgctcgtca tacgctgaac tcgcttcacc aagaaacctc gacacctagg
     1501 agaacccctc atgtccacac ccgcatcact tccgtatgcc ttcgtcgcca agatcgtcgc
     1561 ggccgatgga cagcacgacg cgctcgccga tctgctcgcc ggcgctgtcg cgctcgccaa
     1621 cgaagaagta ggaacgattg tctggttcgc ggtcaggacc cacgccgaca ccttctggat
     1681 cttcgatgca ttccccgacg aggccgctcg cgacgcccac gccaacggcg ccatcgtcgc
     1741 agcactgctg gccaaccagc acct
//
LOCUS       DAOOMA010000601         1758 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_86660_length_1758_cov_2.906048, whole genome shotgun sequence.
ACCESSION   DAOOMA010000601 DAOOMA010000000
VERSION     DAOOMA010000601.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 1758)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1758)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1758
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_86660_length_1758_cov_2.906048"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..314
                     /locus_tag="PLP95_12995"
     CDS             <1..314
                     /locus_tag="PLP95_12995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB46766.1"
                     /translation="APIEDSLYGVAGVDLCVAYRVGDDSVAAVTMRPGTRLCADALAG
                     VEVSYVRVVDGIPHTDWWRLQANKLRDQGIVTSTRRRPVFARQPDGTFVEYTRERAAS
                     A"
     gene            344..>1758
                     /locus_tag="PLP95_13000"
     CDS             344..>1758
                     /locus_tag="PLP95_13000"
                     /inference="COORDINATES: protein motif:HMM:NF012935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase N-terminal
                     domain-containing protein"
                     /protein_id="HPB46767.1"
                     /translation="MDEFDYVVVGGGSAGCVVAGRLSEDPTVRVCLLEAGGEGRDVLI
                     RAPLGFAAAMPRGINSWNYSTVPQPGFNGRRGFQPRGKALGGSSTINAMIYARGHRSD
                     YDEWLALGNPGWGYDDVLEYFKKSECNAIHRDSPFHGTDGPLHVTNLRSPSPLNDVFL
                     EACQAHGIPFNPDLNGADHHGCYHVQVTQKDGERHSTAAAFIHPNLDRPNLDVRTGAH
                     ISRVRFDQRRATAVDYERGGRRESVRARREIIMSAGAFGTPQILMASGIGPGAHLQEL
                     GISTILDLPGVGQNLQDHISALLIYRAKIPDHTVGISPVGVARLLKGMWDWRRHRRGV
                     ITSCAAESGVYYRTSPDVEVSDMEMELIVGIGDDHGRKLHLGHGYSCHVCVLRPKSVG
                     HLKLASADPFATPLIDPAFLDHPDDLETMVAGYKLTKRLLDSPPLASLGAKDMWTGGV
                     RTDDDTRAILRARVDTVYHPIG"
BASE COUNT          294 a          617 c          567 g          280 t
ORIGIN      
        1 ccgccccgat cgaagactcg ctgtacgggg tcgcgggcgt cgacctgtgc gtcgcctacc
       61 gggtcggcga cgactcggtg gcggcggtga ccatgcgccc agggacgcgg ttgtgcgcag
      121 acgcgctcgc cggggtcgag gtgtcctatg tgcgtgtggt cgacgggatt cctcacaccg
      181 actggtggcg tctgcaggcc aacaagctcc gcgatcaggg aatcgtgacc tcgacccgtc
      241 gccgtccggt gttcgcccgc caacccgatg gcaccttcgt cgaatacacc cgggagcgcg
      301 ccgcgagcgc ctgatcgacg tcggtgttga ggtggttcgc tccgtggacg agttcgacta
      361 tgtggtcgtg ggtggaggtt cggcgggctg cgtcgtagcc ggccgcctca gcgaggatcc
      421 gacggtgcgg gtgtgtctac tcgaggccgg cggggagggc cgcgacgttt tgatccgggc
      481 ccctctcggg ttcgcagcgg ccatgcctcg gggcatcaac agctggaact attcgacggt
      541 gccccaacct gggttcaacg ggcggcgcgg gttccaaccg cgcggcaagg cactcggtgg
      601 gtcctccacg atcaacgcga tgatctacgc ccgcgggcac cgcagcgact acgacgagtg
      661 gcttgccctc ggcaacccgg gttggggcta cgacgacgtc ctcgagtact tcaagaaatc
      721 cgagtgcaac gccatccacc gggactcgcc attccacggc accgatggcc cgctccacgt
      781 gacgaatctg cgcagcccga gccccctcaa cgacgtcttc ctcgaggcat gccaggccca
      841 cggaatcccg ttcaaccccg acctgaacgg cgccgaccat cacggctgct accacgtgca
      901 ggtcacccag aaggacggcg aacgccacag caccgcggcc gcgttcatcc acccgaacct
      961 cgaccgcccc aacctcgatg tgcgcacggg agcgcacatc tcccgggtgc gcttcgacca
     1021 acgacgcgcg accgcggtcg actacgagcg cggcggacgg cgcgaatcgg tccgggctcg
     1081 ccgcgagatc atcatgtcgg ctggggcgtt cggaaccccg cagatcctga tggcgtcggg
     1141 aatcggcccc ggcgctcacc tgcaggaact cggcatctcg acgatcctcg acctccccgg
     1201 agtcggacag aaccttcagg atcacatctc ggcgttactc atctatcggg ccaagatccc
     1261 cgaccacacc gtggggatct ccccggtcgg cgtggcacgc ctgctcaagg gaatgtggga
     1321 ctggcgacgc catcgccgcg gcgtgatcac gagttgcgca gccgagtcgg gggtctatta
     1381 ccgcaccagc cccgacgttg aggtctccga catggagatg gaactcatcg tcggcatcgg
     1441 cgacgaccac ggccgcaagc tccatctcgg ccacggctat tcgtgccatg tctgcgtgct
     1501 gcggcccaag agcgtcggcc atctgaaact cgccagcgcc gatccgttcg ccacgccgct
     1561 gatcgacccc gcctttctcg atcatcccga tgatctcgaa acgatggtcg cgggctacaa
     1621 gctgacgaaa cggctgctcg acagcccgcc gctggcctcg ctcggcgcca aggacatgtg
     1681 gacggggggc gtcaggaccg acgacgacac ccgcgccatc ctgcgggcgc gcgtcgatac
     1741 ggtctaccac ccgatcgg
//
LOCUS       DAOOMA010000602         1752 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_87097_length_1752_cov_4.766058, whole genome shotgun sequence.
ACCESSION   DAOOMA010000602 DAOOMA010000000
VERSION     DAOOMA010000602.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 1752)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1752)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1752
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_87097_length_1752_cov_4.766058"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            97..306
                     /locus_tag="PLP95_13005"
     CDS             97..306
                     /locus_tag="PLP95_13005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020379485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HPB46768.1"
                     /translation="MTTTTYTVDGMTCGHCEAAVRQEVEALDGVTVLQVSAADKVLVV
                     ESDEVLDDAAVIAAVDEAGYRAARV"
     gene            309..506
                     /locus_tag="PLP95_13010"
     CDS             309..506
                     /locus_tag="PLP95_13010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB46769.1"
                     /translation="MAGEASVRTRLGAYVVALVVVFGASFGIGRVVVPDGAGQRWQER
                     IESDHHTGTDASQEAEDHDEH"
     gene            496..>1752
                     /locus_tag="PLP95_13015"
     CDS             496..>1752
                     /locus_tag="PLP95_13015"
                     /inference="COORDINATES: protein motif:HMM:TIGR01494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HPB46770.1"
                     /translation="MSTDATLHTIDLDIGGMTCASCAMRIEKKLNKVDGVSASVNYAT
                     EKAHVEVPEGVSVEDLIHVVEQTGYSASQPRPVGNDANPADGIATSGHDLELIALRQR
                     LVGAVVLSVPVIAMAMVPALQFTYWQWLSLTLTAPVVVWAAWPFHRAALINLRHGAAT
                     MDTLISVGTTAAFVWSLYALFFGTAGEPGMTHAFELSVAPTDGAANIYLEAAAGVITF
                     VLAGRYFEKTSKRQAGAALNALLELGAKEVSVLRSPDDVEVTIAIDELVVGDRFVVRP
                     GEKIATDGLVVDGASAVDESMVSGESVPVDVAVGDRVIGATVNQSGRLVVEATRVGAD
                     TQLAQMARLVAEAQSGKAEVQRLADRIAGVFVPVVIAVAVATLGVWLGGGFGISAAFT
                     AGVAVLIIACPCALGLATPTALLVGTG"
BASE COUNT          282 a          535 c          638 g          297 t
ORIGIN      
        1 agtggtcgtg atcacgacca ctggttatcg gaaatggggc tgggtggcgg gaatataccc
       61 taggggggta tggttggggt gatcaggagg tcgatcatga ctacgacgac atacacggtg
      121 gatggaatga catgtgggca ctgcgaggcg gcggtgcgac aggaggttga ggcgctcgat
      181 ggcgtcacgg tgctgcaggt gagcgccgcg gacaaggtgc tcgtggtcga atccgacgag
      241 gtactcgacg atgcagcagt gatcgcggca gtcgacgaag ccggctaccg cgctgcgcgg
      301 gtctgatcat ggccggcgag gcgtcggtgc gcacgcggct cggcgcgtat gttgtcgcgc
      361 tcgtggtggt gttcggcgcg tccttcggaa tcggacgggt cgttgtgcct gacggtgccg
      421 gacagcggtg gcaggagcgc atcgagtccg accatcacac cggcaccgac gcctcgcaag
      481 aggcggagga ccacgatgag cactgacgca accctgcaca cgatcgatct cgacatcggc
      541 ggcatgacgt gcgcctcgtg cgcgatgcgg atcgaaaaga agctgaacaa ggtcgacggg
      601 gtgagcgcgt cggtcaacta cgccaccgaa aaggcgcacg ttgaggtgcc cgagggcgtg
      661 tcggtcgagg atctgattca cgtcgtcgaa cagaccgggt acagcgcgag ccaaccccgc
      721 cccgtcggca acgatgccaa ccccgccgac ggcatcgcga cgagcggtca cgacttggag
      781 ttgatcgcgc tgcgccagcg tctcgtcggt gcggtcgtgc tgtcggttcc ggtgatcgcc
      841 atggcgatgg tgccggcgct gcagttcacc tactggcagt ggttgtcgct gaccctcaca
      901 gcgcccgtgg tcgtgtgggc ggcgtggccg tttcaccgtg cggcgctgat caatctccgt
      961 cacggcgcgg cgacgatgga caccctcatc tcggtcggca cgaccgcagc gtttgtgtgg
     1021 tcgctgtacg cactgttctt cggcaccgcg ggtgagcccg ggatgacgca cgcgttcgaa
     1081 ctgtcggtcg ccccgaccga cggagcggcc aacatctacc tcgaagcggc ggcgggcgtg
     1141 atcaccttcg tgttggccgg ccggtacttc gaaaagacct cgaagcgtca agccggtgcg
     1201 gcgctgaacg cactcctcga actcggggcc aaggaagtct cagtgcttcg ctcaccggac
     1261 gacgtcgagg tcaccatcgc gatcgacgaa ctcgtcgtcg gcgaccgctt cgtcgtgcgg
     1321 cccggcgaaa agatcgccac cgacggcctc gtggtcgacg gcgcctcagc ggtcgacgag
     1381 tcgatggtga gcggagagtc ggtgcccgtc gacgtcgcag tcggcgaccg tgtgatcgga
     1441 gcgacggtca accagagcgg gcgcctcgtc gtcgaggcga ccagggtcgg cgccgacacc
     1501 cagctcgcgc agatggcccg actcgtcgcc gaggcccagt cgggcaaggc ggaggtgcag
     1561 cgtctcgccg atcgcatcgc cggggtgttc gtcccggtgg tcatcgccgt tgcggtcgcc
     1621 accctcgggg tgtggctcgg aggcgggttc ggaatcagtg ccgcgttcac ggcgggcgtc
     1681 gcggtgttga tcatcgcgtg cccatgcgcg ctcgggttgg ccaccccgac ggccttgctg
     1741 gtcggcaccg gg
//
LOCUS       DAOOMA010000603         1739 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_88076_length_1739_cov_3.480404, whole genome shotgun sequence.
ACCESSION   DAOOMA010000603 DAOOMA010000000
VERSION     DAOOMA010000603.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 1739)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1739)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1739
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_88076_length_1739_cov_3.480404"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1199
                     /locus_tag="PLP95_13020"
     CDS             <1..1199
                     /locus_tag="PLP95_13020"
                     /inference="COORDINATES: protein motif:HMM:NF012493.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HPB46771.1"
                     /translation="ATMPPRIAGLAKRHLSDPTHITIAREVVAEGEAPRVSQTVYYVR
                     RNDKAAALARILDVEVPEAAIVFCRTRTEVDELTEALNGRGYRAEGLHGGMSQEQRDR
                     VMGRVRATTADLLIATDVAARGLDIDHLTHVVNFDVPAAPEQYVHRIGRVGRAGREGT
                     AITIAEPRQQRFIKNIERVTKQHVTIGQIPTVAQLRTRRKELTIEKIRAELVEHLYED
                     YRSIVDSLSDEFDVADIAAAAVRAAHEALGSTHPENEEEIHNVEMRSERRPAGNDRFR
                     KGTDRTLRGPGERRGFDSKRGDRRGFDRDGERPGDRPVQPGYSRIWVSAGRRNGVRPG
                     DLVGAICGETSLRGNQIGAIRITENFSLVEVPSGNADEVIRKLGDTTIKGRRTSVRRD
                     KGPRRD"
BASE COUNT          334 a          592 c          545 g          268 t
ORIGIN      
        1 tcgccaccat gcccccgcgt atcgccgggt tggcaaagcg tcacctcagc gacccgaccc
       61 acatcacgat cgcccgcgag gtcgtcgccg agggcgaggc gccacgggtc agccagaccg
      121 tttactacgt gcgtcgcaac gacaaggccg cagcactcgc ccgcattctc gacgtcgagg
      181 ttcccgaggc ggcgatcgtg ttctgccgca cccgaaccga ggtcgacgaa ctcaccgagg
      241 cgctcaacgg ccgcgggtat cgcgccgagg gcctgcacgg cggcatgagt caggaacaac
      301 gcgaccgcgt catgggtcgg gttcgcgcca cgactgccga tctgttgatc gccaccgacg
      361 tcgctgctcg cggcctcgac atcgatcacc tcacccacgt ggtgaacttc gacgtgccgg
      421 ccgctcccga acagtacgtg caccggatcg gccgcgtcgg tcgtgccggg cgcgagggga
      481 ccgcgatcac gatcgccgag ccgcgccagc agcggttcat caagaacatc gagcgcgtga
      541 ccaaacagca cgtcacgatc ggccagattc cgaccgttgc ccagctgcga acccgccgca
      601 aggaactcac gatcgaaaag attcgcgccg agctcgtcga acacctctac gaggactacc
      661 gctcgatcgt cgattcgctg agcgacgaat tcgacgttgc cgacatcgca gcggctgcgg
      721 tgcgcgccgc tcacgaggca ctcgggtcga cccacccgga aaacgaagag gaaatccaca
      781 acgtcgagat gcgttcggaa cgccgtccgg cgggcaacga tcgtttccgc aagggaaccg
      841 atcggaccct gcgcggcccg ggcgaacgcc gtgggttcga ctcgaaacgt ggcgaccgaa
      901 ggggcttcga tcgcgacggg gaacggccgg gagatcgccc ggtccagccc ggatacagcc
      961 gcatctgggt gagcgccgga cgccgcaacg gtgtgcggcc gggcgacctg gtgggggcga
     1021 tctgcgggga aacctcgctg cgcggcaacc agatcggggc catccggatc accgagaact
     1081 tctccctcgt cgaggttcct tccggcaacg cggatgaggt gatccgcaag ctcggcgaca
     1141 cgacgatcaa gggccgcaga acgagcgtgc gtcgcgataa gggtccgcgt cgagactgac
     1201 gcggccatcg cacggatcgg cgccgcgggc ggcacatggt gacctcgacg aagccgctcc
     1261 atcggcgccg attcccgagt ttcagcacat ttcgcgtcac ctcaaaaccc ttgacgtggc
     1321 gacgtcggct ccgtaacact tccatcgcgg ggcgcctttt tgagaccgcc atcccagcgg
     1381 ttgggttctt tcacccacct gaagccgatc cgcatcaaac ggaggcgacc cgggtaggat
     1441 gggtcgcacg cctcagtatc tgagccgagt ggcccagagt cgttcgaccc agaaaaatga
     1501 gttcgtccgg atcccccctc cggaccttga atttctcatc accgttctgt cacggtgacc
     1561 gagagctaca tgggagttgc agcagtgaat cacaacccaa gtcagaccaa gtcaaagcga
     1621 gtgcggctcg cagcggcatc gatgatcctc ggttcgacga tgatcggcgc ccctttggtc
     1681 gccggtgcac agtcggagga tctcgttccg gccaccaccg atacttcggc cccggtcgc
//
LOCUS       DAOOMA010000604         1735 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_88400_length_1735_cov_3.012500, whole genome shotgun sequence.
ACCESSION   DAOOMA010000604 DAOOMA010000000
VERSION     DAOOMA010000604.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 1735)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1735)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1735
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_88400_length_1735_cov_3.012500"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..843
                     /locus_tag="PLP95_13025"
     CDS             <1..843
                     /locus_tag="PLP95_13025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015798203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HPB46772.1"
                     /translation="AHIVAVRPLRDGVISDFEVCEKMLRYFIQKVNHRRFARPRMVIC
                     VPSGVTGVEQRAVQEAAEYAGAAKPVDIIEEPMAAAIGANQPVHEPRGNMIVDIGGGT
                     TEVAVISLGGVVASQSVRVAGDELDEAIIQFVKKEYSLALGERTAEEVKMYLGSAYPL
                     EQEMQAEIRGRDLVSGLPKTIVTSTEEIRDAIEEPVSAIVDAVKFTLDKTPPELAADI
                     MEQGIVLAGGGALLAGLNTRIANETGMPVRVADNPLDAVALGSGQYLELPEAARGVLH
                     GARG"
     gene            859..1692
                     /gene="mreC"
                     /locus_tag="PLP95_13030"
     CDS             859..1692
                     /gene="mreC"
                     /locus_tag="PLP95_13030"
                     /inference="COORDINATES: protein motif:HMM:TIGR00219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HPB46773.1"
                     /translation="MHGSNPRRPRTTLIIAILASLTVITITAKDVPVIGSVRSAIVDA
                     TSPVNGAFRSATKPLRNWFGSVTDHERVVEENERLRDEVAVLKSKQIANANAATELEA
                     IKEQEGLPTEEHFDSLLAQVATGPYSNFDEHTLMLTRGARDGVKVGSPVVTSVGLVGK
                     IESTTDSTSIVRLITDPEMAISLRIATKGTYVAGHGTGPNSPFVVDYGVPLTEEIAEG
                     ETIVTSGLNTAQFPPHIPVGTVTKVSTSRSDMTQVLEVAYLADFTRLNYVRVLLWVPN
                     K"
BASE COUNT          329 a          568 c          568 g          270 t
ORIGIN      
        1 gcacacatcg tggccgttcg cccgctgcgc gacggcgtga tcagcgactt cgaggtgtgc
       61 gagaagatgc tgcggtattt catccagaag gtgaaccacc gccgtttcgc ccgcccgcgc
      121 atggtgatct gtgtgccgtc gggggtgacg ggggtggaac aacgcgccgt gcaggaggcc
      181 gccgaatacg cgggggccgc caagccggtc gacatcatcg aagagccgat ggccgcggcg
      241 atcggggcga atcagccggt gcacgaaccg cgcggcaaca tgatcgtcga catcggcgga
      301 ggcaccaccg aggttgcggt catctcgctc ggcggtgtgg tcgccagcca gtcggtgcgg
      361 gtcgccggcg acgaactcga cgaagcgatc atccagttcg tcaaaaagga atacagcctc
      421 gcgctcggcg agcgtacggc cgaggaagtg aagatgtacc tcggctccgc ctacccgctc
      481 gaacaggaaa tgcaagcgga aatccgcggc cgcgacctcg tcagcggact gcccaagacg
      541 atcgtcacct ccaccgagga gatccgcgac gccatcgaag aaccggtctc ggccatcgtc
      601 gacgcggtga agttcaccct cgacaagacc ccgccggaac tcgccgccga catcatggaa
      661 cagggcatcg tgctcgccgg cggcggggcg ttgctcgcgg ggctcaacac ccggatcgcg
      721 aacgaaaccg gcatgccggt gcgcgtcgcc gacaacccgc tcgacgcggt ggcgctcggc
      781 tcgggtcagt acctcgaact tcccgaggcg gcgcgaggcg tgttgcacgg cgcccgcggc
      841 taggcctcga cccgacgaat gcacggctcg aacccgcgtc gaccccgcac gacactgatc
      901 atcgccattc ttgcctcgct gacggtcatc acgatcacgg ccaaggatgt gccggtgatc
      961 ggcagcgtgc gatcggcgat cgtcgatgcg acatcgccgg tcaacggcgc gtttcgctcg
     1021 gcgaccaaac cgttgcgcaa ctggttcgga agcgtcaccg accacgagcg tgtggtcgag
     1081 gagaacgagc ggctgcgcga cgaggttgcg gtgctcaagt cgaaacagat cgccaacgcg
     1141 aacgccgcca ccgaactcga agcgatcaag gagcaggagg ggcttccgac cgaggagcac
     1201 ttcgactcgt tgttggcgca ggtggcgacc ggcccgtatt cgaacttcga tgagcacacg
     1261 ctgatgttga ctcgcggcgc tcgcgacggg gtgaaggtcg gaagccctgt cgtgacgagt
     1321 gtcggcctgg tgggcaagat cgaatccacg accgattcga cctccatcgt gcgcctcatc
     1381 accgaccccg agatggccat ttcgctgcgc atcgccacga agggcaccta cgttgccggt
     1441 catggaaccg gcccgaacag ccccttcgtc gtcgattacg gagtcccgct gaccgaggag
     1501 atcgccgagg gcgagacgat cgtcacgagc gggctcaaca ccgcccagtt tccgcctcat
     1561 atcccggtgg gtaccgtgac gaaggtatcg acgagtcgat ccgacatgac ccaggtgctc
     1621 gaggtggcct accttgcgga cttcacgcgc ctcaactatg tgcgcgtgct gctctgggtg
     1681 ccgaacaaat gacccccctc gacgtcgccg cacgctgggg actcgtcgtg gtcat
//
LOCUS       DAOOMA010000605         1730 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_88794_length_1730_cov_3.354627, whole genome shotgun sequence.
ACCESSION   DAOOMA010000605 DAOOMA010000000
VERSION     DAOOMA010000605.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 1730)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1730)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1730
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_88794_length_1730_cov_3.354627"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(212..>1730)
                     /locus_tag="PLP95_13035"
     CDS             complement(212..>1730)
                     /locus_tag="PLP95_13035"
                     /inference="COORDINATES: protein motif:HMM:NF020976.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alkaline phosphatase D family protein"
                     /protein_id="HPB46774.1"
                     /translation="VVFGIASGCAANYVPPPNGTPFTLGVMSGLHAPDAVVLWTRLDP
                     TQAAGTTTVSWQIATDPAMSTVIRRGSSSADPSRDHTVKVLVEGLEPDRSYWYRFDVD
                     GVASRVGRARTLPSPGAAVRSLSVVVASCQNWANGWYHAWKGIAAEDVDAVLWLGDYI
                     YESGTSVLSARVDQVAEATTLEQYRAKYRMYRSDPALQDGHAAHPFVPVRDDHELFND
                     CDRFSMLQFPERAAASYQAWFDYMPVMPIDGTRVYRSYRWGDLAVIPMLDSRQYRDRG
                     ANGFQGSTKPPIVGIGDVVREAQLPGRSMLGEEQRGWLLDTIDEAQAEGVRWKLLGNP
                     VMMTPTRAIDLDEPGIRKLFPDLPRNEGLFINMDAWNSYLWERQLLFDHWHANSVDSI
                     AVLTGDIHSFWRARQRLDMEDEASPVVATEYVTGSISSTTPNILRTPEFGRFLESGPV
                     QWIPRFDYVDFRHNGYGVVRATPERLDVQFRRTLATERYASVTDGMSFSEYFSEY"
BASE COUNT          318 a          578 c          558 g          276 t
ORIGIN      
        1 actctcactc accccgacca aaccacccca accaccgata cgccaatgaa ccggcagtga
       61 ccgaaacgag cgatgcaccc tcaaggggac gaaccctgcg aaccctgtca accctgtcaa
      121 ccctgtcaac ggccccgccc ggggtgtgtt ggcgcgcccg cacacggtgc cggtgctggt
      181 gctggtgctg gcgagtagga actgccgggt atcagtactc gctgaagtac tcgctgaagg
      241 acataccgtc ggtcacgctg gcgtaccgtt cggtcgccag cgtgcggcgg aactgcacgt
      301 cgaggcgctc cggcgtcgct cgcacaaccc catagccgtt gtgacgaaaa tcgacgtagt
      361 cgaaccgtgg aatccactgc acggggcccg attcgaggaa ccgcccgaac tcgggggtgc
      421 gcaagatgtt gggagtcgtc gacgagatcg agccggtgac gtactcggtc gcgacgaccg
      481 gcgaggcctc gtcctccata tcgagccgtt gccgcgcccg ccagaagctg tggatgtcgc
      541 cggtcagcac cgcgatcgag tcgaccgagt tcgcgtgcca gtggtcgaac agcaactgcc
      601 gttcccagag ataggagttc cacgcgtcca tgttgatgaa caggccttcg ttgcgaggaa
      661 ggtcggggaa cagtttccgg atgccgggtt cgtcgagatc gatcgcccgc gtcggtgtca
      721 tcatcaccgg gttgccgagc agcttccagc gaacaccctc ggcttgggcc tcatcgatgg
      781 tgtcgagcag ccacccccgc tgctcctctc ccagcatcga ccggccgggg agttgcgcct
      841 cgcggaccac gtcgccgatt ccgacgatgg ggggcttggt cgaaccctga aagccgttcg
      901 ccccgcgatc gcggtactgg cgactgtcga gcatcgggat cacggcgagg tcgccccagc
      961 gataggaacg gtagacgcgt gtaccgtcga tcggcatcac cggcatgtag tcgaaccacg
     1021 cctgatacga cgctgcggcc cgctcgggga actgcagcat cgagaaacgg tcgcagtcat
     1081 tgaacagttc gtggtcatcg cgaaccggca cgaacggatg cgcggcgtgg ccgtcttgga
     1141 gcgccgggtc gctgcgatac atccgatact tggcccggta ctgctcgagc gtcgtggcct
     1201 cggccacctg gtcgacacga gccgacaaca ccgacgtgcc gctttcgtag atgtagtcgc
     1261 cgagccacaa cacggcgtcg acgtcttcgg cggcgatccc cttccaggcg tgataccagc
     1321 cattcgccca gttctgacac gaggccacga ccaccgacag cgaccgcacc gcagcgcccg
     1381 gactgggaag ggtccgagcg cgtccgaccc ggctggcaac gccatcgacg tcgaagcggt
     1441 accaatagga gcggtcgggt tcgaggccct cgacgagcac cttcacggtg tgatcccgcg
     1501 acggatctgc gctcgaggac ccccgccgaa tcaccgtcga catcgccggg tcggtggcga
     1561 tctgccagct gacggtggtc gtaccagcgg cctgggtcgg gtcgagacgg gtccacagca
     1621 cgaccgcgtc gggggcgtgc aacccggaca tgactccgag ggtgaacggc gtcccgttcg
     1681 gcggcggcac gtagttcgcg gcacagccgc tggcgatacc gaacacgacc
//
LOCUS       DAOOMA010000606         1723 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_89350_length_1723_cov_3.601319, whole genome shotgun sequence.
ACCESSION   DAOOMA010000606 DAOOMA010000000
VERSION     DAOOMA010000606.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 1723)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1723)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1723
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_89350_length_1723_cov_3.601319"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1634
                     /locus_tag="PLP95_13040"
     CDS             <1..1634
                     /locus_tag="PLP95_13040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HPB46775.1"
                     /translation="LAIGLSFIGGMGIAAALTVAVTMVASVTLLPALLGFAGHRVELT
                     RWRGLIAAGFVALGLLGVGLNFTPMVAAFGVAVAILVAGFFVAPLRAEVRQRRPKPAE
                     QTLAYKWSHLIQRHPWSAMLAGVAVLLVVASPVLSLRLGSADAGNAPEDTDARRAYDL
                     LSEGFGPGFNGPLIVTVRGVNMIAAGGIGGLLEGTDGVAQVVGPIPSNLQDLQASGLM
                     EFMSGGAAGGAAGGTEAGAAGDPTAGGLLPSDPEELLAFDLSTLTSSEFAPLLPDNVE
                     ELLSPSALMFQVFPTTSPQDEETVALIERIRDEVVGGVRGVVDGAELHVTGNTAINVD
                     FTTYLSERMVMFFSIVLALSFLLLMMVFRSILVPIKAVIMNLLSIGAAYGVVVAVFQW
                     GWGSSLFGVSPAPIEPFIPVMMFAIVFGLSMDYEVFLLSRIKEEYDRSGDSRNSVADG
                     LAATARVITAAAAIMVVVFGSFLFEDERVVKSFGVGLAVAVFLDATLVRMLLVPATME
                     LLGDKNWWFPRWLGRILPRLNVEGPAHDLDVTHDA"
BASE COUNT          228 a          514 c          615 g          366 t
ORIGIN      
        1 tgttggcgat cgggctgtcg ttcatcggtg ggatggggat cgctgcggcg ctcacggtgg
       61 cggtgacgat ggtcgcctcg gtgaccctgt tgccggcgct gctgggattc gccggtcatc
      121 gggtagagct gacgcggtgg cgcgggctga tcgccgcagg gttcgtggcg ctcggcctgc
      181 tcggcgtcgg cctgaacttc actccgatgg tggcggcatt cggcgtcgcg gtcgccatcc
      241 tggtcgccgg gttctttgtt gcgccgctgc gtgcggaggt tcgccagcgt cgccccaagc
      301 ccgctgaaca gacccttgcg tacaagtgga gtcacctcat ccagcgtcat ccgtggagtg
      361 cgatgctcgc cggggtggcg gtgttgctgg tggtcgcatc gccagtgctg tcgttgcgcc
      421 tcggatccgc ggatgcgggc aacgctcccg aggacaccga cgcccggcgg gcctatgacc
      481 tgctgtcgga gggtttcggg ccggggttca acggtccgct gatcgtgacg gtccgcgggg
      541 tcaacatgat cgccgccggc ggaatcgggg ggctgctcga gggcaccgac ggcgtcgccc
      601 aggtggttgg tccgatcccg tcgaatctgc aggacctcca ggcctcgggg ttgatggagt
      661 tcatgtcagg cggtgcggcg ggcggtgcgg cgggcggtac agaggccggt gcggcggggg
      721 atccgaccgc cggcggcctg ttgccgagcg accccgaaga actgttggcc ttcgacctct
      781 caacgctgac gtcatcagag ttcgccccgc tgcttcccga caatgtcgag gagttgctga
      841 gcccgtcggc gttgatgttc caggtgtttc ccacgacctc gccacaggat gaagagacgg
      901 tcgcgttgat cgagcgcatc cgtgacgagg tggttggagg ggtccgcggc gtcgtcgacg
      961 gtgcggagct gcacgtcacc ggcaacaccg cgatcaacgt ggacttcacg acctacctgt
     1021 ccgagcgcat ggtgatgttc ttcagcatcg tgttggcgct gtcattcctg ctgttgatga
     1081 tggtcttccg ctcgattctc gtgccgatca aggcggtcat catgaacctg ttgtcgatcg
     1141 gcgctgccta cggcgtcgtg gtggcggtgt tccagtgggg atggggttct agcctgttcg
     1201 gtgtctcgcc agcgcctatc gaaccgttca ttccggtgat gatgttcgcc atcgttttcg
     1261 ggttgtcgat ggactacgag gtattcctcc tgtcgcgaat caaagaggaa tacgaccgct
     1321 ccggcgactc acgtaactcg gtggccgacg gtctggcggc gacggcccgg gtgatcaccg
     1381 ccgcggcggc gatcatggtg gtggtgttcg gttcgtttct gttcgaggat gagcgggtcg
     1441 tcaagagctt cggtgtgggg ctggccgttg cggtgtttct cgacgcgacc ctggtgcgga
     1501 tgttgctggt tccggcgacg atggaactgc tcggtgacaa gaactggtgg ttcccccgct
     1561 ggctcggtcg gatcctgccg cggctcaatg ttgagggccc ggcccacgac cttgacgtga
     1621 cccacgacgc ctgagcccct gcccggccca gcctcgcgcc cggcccagcc ggttctccga
     1681 taaccagtgg tcgtgatcac gaccactggt tatcggagaa ccg
//
LOCUS       DAOOMA010000607         1714 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_90074_length_1714_cov_4.241109, whole genome shotgun sequence.
ACCESSION   DAOOMA010000607 DAOOMA010000000
VERSION     DAOOMA010000607.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 1714)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1714)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1714
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_90074_length_1714_cov_4.241109"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..456)
                     /locus_tag="PLP95_13045"
     CDS             complement(<1..456)
                     /locus_tag="PLP95_13045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012228867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HPB46776.1"
                     /translation="MTLRLATPADLLDHVGDQLGPTDAVSVDQDRINQFAEATGDHQW
                     IHVDVERANAESPFGGPIAHGYLTLSLANLFLPELLTVENFSMGVNVGVDKVRFPSPV
                     PAGATISATGEILSAEQIGEGIQVVVRITISVDGAAKPSCIVDTVSRFFP"
     gene            complement(453..842)
                     /locus_tag="PLP95_13050"
     CDS             complement(453..842)
                     /locus_tag="PLP95_13050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB46777.1"
                     /translation="MLAVSAFAALVAVGAASGCGSSEPSRDAFIAEMRTTLGSDLAEG
                     ASLDDVDPDDLRTVVDEFLGCTYDAVKDDSELLRQMYRDPSFSTATTGSSDNSGQLRS
                     RADQLSAECVTELQESTNQLVSKDSSQ"
     gene            complement(907..1689)
                     /locus_tag="PLP95_13055"
     CDS             complement(907..1689)
                     /locus_tag="PLP95_13055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HPB46778.1"
                     /translation="MAAPVPDYPPARGLLTDNTVVVTAAAGTGIGFSLAKRCTEEGAT
                     VLISDLHERRLAEAADRIEVETGRRPFTQLCNVTDEEQVQALVAAGIEQLGHIDVWMN
                     NAGLGGNAPLVEMTDDQWNVVLDVTLTGTFRCMRAVLPHMYERNAGVVVNNASVLGWR
                     AQELQAHYAAAKAGVMALTRCSAMEAAKHNVRVNAVSPSLAMHPFLAKVTPDGLLEEL
                     EAREAFGRAAEPWEIANVMVMLASDYTTYMTGEVVAVSSQHA"
BASE COUNT          272 a          517 c          617 g          308 t
ORIGIN      
        1 ggggaagaaa cggctgacgg tgtcgacgat gcacgagggt ttcgctgcac cgtcgacgct
       61 gatcgtgatg cgcacgacca cctggattcc ctcgccgatc tgttccgccg acaggatctc
      121 gccggtcgcc gagatcgtcg ccccggccgg cacgggtgaa gggaaccgca ccttgtcgac
      181 cccgacgttg acccccatgg agaagttctc gaccgtgagc aactcgggca ggaagaggtt
      241 ggcgagcgac agcgtgagat agccgtgggc gatcgggccg ccgaaggggc tctcggcatt
      301 cgcccgttcg acgtccacgt ggatccactg atggtccccg gtcgcctcgg cgaactggtt
      361 gatgcggtcc tggtcgacgc tcaccgcgtc ggtggggccg agttggtcgc cgacgtggtc
      421 gagcaggtcg gcaggggtgg cgagtcgaag cgtcattgcg aggaatcctt ggacacgagt
      481 tggttggtcg attcttggag ttcggtcacg cactctgcgc tcaattgatc ggcgcgcgag
      541 cggagttggc ccgaattatc ggagcttccg gtggtcgcgg tgctgaacga cgggtcgcga
      601 tacatctggc gaagcaattc ggaatcgtcc ttcaccgcgt cgtaggtgca gccgaggaac
      661 tcgtcgacca cggtgcgcag atcgtcgggg tcgacgtcgt cgaggctcgc tccctcggcc
      721 aggtcggaac cgagggtggt gcgcatctcc gcgatgaacg cgtcgcgcga cggttccgat
      781 gagccgcacc cgctcgcagc accgacagcg acgagcgcgg cgaacgcgga caccgcgagc
      841 atgcggcgag ccgttcgacg gctctcggat cggggtcgag gggggaggtg gatcacgacc
      901 gtcaggctag gcgtgctggg acgatacggc gacgacctcg ccggtcatgt aggtcgtgta
      961 gtcgctggcc aacatgacca tgacgttcgc gatttcccag ggctctgcgg cccggccgaa
     1021 ggcctcgcgg gcttcgagtt cctcgagcag accgtcggga gtgaccttgg cgagaaacgg
     1081 gtgcatggcg agcgacggcg acacggcgtt gacccgcacg ttgtgcttgg cggcttccat
     1141 cgccgaacag cgggtcagtg ccatcacacc ggccttggcc gcggcgtagt gggcctgcag
     1201 ttcttgcgct cgccagccca gtaccgacgc gttgttcacc acgacaccgg cgttgcgttc
     1261 gtacatgtgg ggcagcacgg cgcgcatgca ccgaaacgtg ccggtgaggg tgacgtcgag
     1321 gacgacgttc cactgatcgt cggtcatctc gacgagcggg gcgttaccac ccaaacctgc
     1381 gttgttcatc cacacgtcga tgtggccgag ttgttcgata cccgcagcga cgagcgcttg
     1441 cacctgttcc tcgtcggtca cgttgcagag ttgggtgaac ggacggcgac cggtttcgac
     1501 ctcgatgcga tcggcggcct cggcgaggcg ccgttcgtgc aggtcgctga tgaggaccgt
     1561 cgcgccttcc tcggtgcagc gcttcgcgag ggagaacccg atgccggtgc cggcggccgc
     1621 ggtgaccacc acggtattgt cggtgaggag gcctcgtgcg ggtgggtaat cgggaacggg
     1681 tgcggccatg gggattcctc cggcgggtgc ggtt
//
LOCUS       DAOOMA010000608         1714 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_90075_length_1714_cov_3.898734, whole genome shotgun sequence.
ACCESSION   DAOOMA010000608 DAOOMA010000000
VERSION     DAOOMA010000608.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 1714)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1714)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1714
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_90075_length_1714_cov_3.898734"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..269)
                     /locus_tag="PLP95_13060"
     CDS             complement(<1..269)
                     /locus_tag="PLP95_13060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015439742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB46779.1"
                     /translation="MTEAWIIDAVRTPRGGGKPGKGALTGHHPQHLGAAVLKAIAERN
                     DLNTAEVDDIIFGTSRQVGTQGGDLGRMAELDARAAVRLGQEAER"
     gene            complement(303..1484)
                     /locus_tag="PLP95_13065"
     CDS             complement(303..1484)
                     /locus_tag="PLP95_13065"
                     /inference="COORDINATES: protein motif:HMM:NF023568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HPB46780.1"
                     /translation="MGHKVETIAYPIVRVEQAAFKDTYGGDEGVVLCESYLLKPVGVD
                     SDTVIVFMHPVGAGAYLPMVRGLAKAGHHVIYCNSHYRADFALIMEKVVLDLGACIRD
                     AKQRLGYAKVVLAGWSGGGSLSLFYQSQAEHPTLTETPAGDPPDLTAADLIPADGIML
                     LAAHPSRHGTLTLSLDPAVRDESNPDDRDPAFDLYGPDAPQPPYDREWLERYRAAQVA
                     RNRRITAWVKAKLAELRASGRDNEEFAFVVHGTMADPAALDGTIDPNDREVGVSFLGD
                     PRVVNNGPVGLARFCTLRSWLSQWSLDDANGDGPRAAATISIPALVIYNSADNICLPQ
                     FALDLYDAIGHEDKELHCIDGATHYYLGDDQRPKLAEAVGIITRWLTDHDFGSPATDL
                     E"
BASE COUNT          245 a          532 c          615 g          322 t
ORIGIN      
        1 cttctctcag cttcctgtcc gaggcgtact gcagcacggg cgtccagctc ggccatgcgc
       61 ccgaggtcgc cgccctgggt tccgacctgt cggctcgtgc cgaaaatgat gtcgtcgacc
      121 tcggcggtgt tgaggtcgtt gcgctcggcg attgccttga ggaccgcggc gccgaggtgc
      181 tgcgggtgat gtccggtgag ggcacccttg cccggcttac ccccgcctcg cggcgtgcga
      241 accgcatcga tgatccatgc ttcggtcatg tgtccaaacc ccctggagct aatttgagaa
      301 tcctattcta aatctgtggc cggcgagccg aaatcgtggt cggtgagcca acgggtgatg
      361 atgcccacgg cctcggcgag cttcgggcgc tggtcgtcgc cgaggtagta gtgggtcgcc
      421 ccgtcgatgc agtgcagttc cttgtcctcg tggccgatgg cgtcgtagag gtccaacgcg
      481 aactggggca gacagatgtt gtcggcgctg ttgtagatga ccagcgccgg aatcgagatc
      541 gtcgccgcag cgcgcggccc gtcgccgttg gcgtcgtcga ggctccactg cgacagccaa
      601 ctgcgcagcg tgcagaaccg ggcgaggccg acggggccgt tgttgaccac ccgcggatca
      661 ccgaggaacg acacgccgac ctcgcggtcg ttcgggtcga tggtcccgtc gagcgcggcc
      721 ggatccgcca tcgtgccgtg cacgacgaat gcgaactcct cgttgtcccg gccggaggcc
      781 cgaagttccg cgagcttggc cttcacccat gcggtgatac gccggttccg tgccacttgc
      841 gccgcacggt agcgctcgag ccactcgcgg tcgtaggggg gttgtggggc gtcggggccg
      901 tagagatcga aggccgggtc gcggtcgtcc gggttcgact cgtcgcgtac cgcggggtcg
      961 agcgacaggg tgagggtgcc gtggcgcgac gggtgcgcgg cgagcagcat gatcccatcg
     1021 gcggggatga ggtctgcggc ggtgaggtcg ggcggatcgc ccgccggcgt ttcggtgagc
     1081 gtcgggtgtt cggcctgcga ttggtagaac agcgacagcg aaccgcctcc cgaccacccg
     1141 gcgagcacga ccttggcgta gcccaggcgt tgcttggcgt cgcggatgca ggcgccgagg
     1201 tcgagcacga ccttttccat gatgagggcg aagtcggccc ggtagtggct gttgcagtag
     1261 atgacgtgat gtcccgcctt ggcgagaccg cgcaccatcg gcaggtacgc gcccgctccg
     1321 accggatgca tgaacacgat gaccgtgtcg ctgtccaccc cgaccggctt gaggagatac
     1381 gactcgcaca acacgacgcc ctcgtcaccg ccgtaggtgt ctttgaacgc tgcctgttcg
     1441 accctaacga tcgggtacgc gatggtctcg actttgtggc ccacgaggcc cccctgatgt
     1501 ggtgttgtgg tgtcgccttg cgtgttgcgt gttgcgtgtt gcgtgcggcg tggactcaga
     1561 ccatgtcggc ggggcgcagg tcgaatccga gcgcgacccg gccggcgagt tcggcggtgc
     1621 ggtcccagtc gaagatgacg tgacccttca gggtttccag gtcgcgttgg atgcgctgga
     1681 gcgggtgatc gctgcggtac acgctggcgc ccgc
//
LOCUS       DAOOMA010000609         1709 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_90489_length_1709_cov_2.492745, whole genome shotgun sequence.
ACCESSION   DAOOMA010000609 DAOOMA010000000
VERSION     DAOOMA010000609.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 1709)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1709)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1709
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_90489_length_1709_cov_2.492745"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..879
                     /locus_tag="PLP95_13070"
     CDS             <1..879
                     /locus_tag="PLP95_13070"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HPB46781.1"
                     /translation="NFWPEMNRTGATITSTLGTMAYLLAHDEDRPEMPRSGSESANSS
                     LRLLAAAPLPPEVDETIKGRFGVATFSGGYGVTEASMISWLPPGVTNRPAAAGVVNDA
                     YFDVRIFDDDDNELPTGTDGEIVIRPKRPQVMFEGYWGRPDATVETSRNWWYHTGDIG
                     RIDEDRFLYFVDRKADYLRRRGENISSFEVERILMKHPAIADVAVHAVFSELTEDDLK
                     VTATIAVGEEVSEEELFRWCLDELPYFALPRYVEFRAELPRSPVGRVLKRELRDEGVT
                     AATWDVEAAGITYERR"
     gene            1150..>1709
                     /locus_tag="PLP95_13075"
     CDS             1150..>1709
                     /locus_tag="PLP95_13075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB46782.1"
                     /translation="MRKRCDIAVLAFIAAAMLSGCGDESEEIGRRSEPQSSPISGVPN
                     VDVEPMNELTWGPLVASAAVEVVVADTELTPEVDGERKWRAVGRVFWRNTGEENWVET
                     PQLSVERSAISALAVREREVLVAVAECPSDFAQDATQPCGSAQLQTLDLESREWSRRA
                     DLPGGDNESLVGLWGTASGWIAATQRL"
BASE COUNT          309 a          485 c          591 g          324 t
ORIGIN      
        1 aatttctggc cggagatgaa ccggaccgga gcgacgatca cctccacgct cggcacgatg
       61 gcgtacctgt tggctcacga cgaggaccgt ccagagatgc cgcggtcggg gtcggagtcg
      121 gcgaactcct cactgcggtt gttggccgcg gcgccattgc cccccgaggt tgacgagacg
      181 atcaagggtc gcttcggggt cgccacgttc agcggtggct acggggtgac cgaggcgtcg
      241 atgatctcgt ggctgccgcc cggtgtgacc aaccgacccg ctgcggccgg tgtggtgaac
      301 gacgcctatt tcgacgtgcg gatcttcgac gatgacgaca acgagttgcc caccggtacc
      361 gacggcgaga tcgtcattcg gcccaagcgc ccccaggtga tgttcgaggg gtactggggt
      421 cggccagatg cgacggtcga gacgagtcgg aactggtggt atcacaccgg cgacatcggc
      481 cggatcgacg aggaccggtt cctgtacttc gtcgatcgca aggccgacta cctgcggcgg
      541 cgcggcgaga acatctcgag cttcgaggtc gaacggatcc tcatgaagca cccggccatc
      601 gcggacgtcg cagtgcatgc ggtgttcagc gaactcaccg aggatgacct caaggtcacc
      661 gcgacgatcg cagtcggtga ggaggtgagc gaggaggagc tgtttcgctg gtgcctcgac
      721 gaattgccgt atttcgcgct gccgcgctac gtcgagttcc gcgccgagtt gccgcgcagc
      781 ccggtcgggc gggtgctcaa gcgggagttg cgtgacgagg gggtcacggc ggcgacgtgg
      841 gatgtcgaag cggcggggat cacctacgag cgtcgctgat ctcgacaaca ttggcgggca
      901 cgagtgtggt cgctgtaccc acacgcggcg ctcaaacatg agcgacgacg cctgttccgc
      961 cggcctgtac tttcaccttg cgcgcgctgg tagctttcat ccgtcaagtg ccttcctcgt
     1021 tactcctctg gccgcttcat aacacccaga aaggcccacg agttagggca cctgtcgtga
     1081 tcgggaactc tcaacccata tcaccccgtg aaggatcacg ggttagagca aagtctagga
     1141 gagtgaggca tgcgtaagag atgcgatatt gcggtcttgg ctttcattgc tgcggcaatg
     1201 ctctccggct gtggtgatga gtccgaggaa attggacgac gatcagaacc tcagtcgtca
     1261 ccgatctcag gcgttccaaa tgtcgacgtt gagccgatga atgagttgac gtgggggccg
     1321 ctcgtggcgt cggcagcggt cgaagtcgtg gtcgctgata cggagttgac gcccgaggtc
     1381 gacggcgagc ggaaatggcg tgctgtcggg cgggtcttct ggcgcaacac aggtgaagag
     1441 aattgggttg aaacaccgca attgagcgtg gagcggtcgg cgatctccgc gctggcggtg
     1501 cgggagcgcg aagtgctcgt ggcggtcgcc gagtgtccgt ctgatttcgc ccaggatgca
     1561 actcagcctt gcggatccgc tcaactgcaa acgctcgatc tggagtcgag ggagtggagt
     1621 cggcgcgccg atcttcctgg tggagacaat gagtcgctcg tcggcctttg gggtacggcg
     1681 agcggttgga tcgcggccac acaacggct
//
LOCUS       DAOOMA010000610         1702 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_91064_length_1702_cov_2.541591, whole genome shotgun sequence.
ACCESSION   DAOOMA010000610 DAOOMA010000000
VERSION     DAOOMA010000610.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 1702)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1702)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1702
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_91064_length_1702_cov_2.541591"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            100..>1702
                     /gene="otsB"
                     /locus_tag="PLP95_13080"
     CDS             100..>1702
                     /gene="otsB"
                     /locus_tag="PLP95_13080"
                     /EC_number="3.1.3.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004805 - trehalose-phosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-phosphatase"
                     /protein_id="HPB46783.1"
                     /translation="MNGWSDELSEALAAAARSPQLLVACDYDGTLAPIVDDPAAAHPI
                     RAAMTALRGLALVPDTSVSVISGRALRDLALLTRLPDEVHLVGSHGSEFEPHDTGRLG
                     QAEIALLHDVTREMLAIASNYPGCSVEPKPVGAAFHYRRADAHVGATAAAEVLAGPAR
                     RAGVHVREGKAVVELSVVDTDKGAALDRLRSILGADTVIFLGDDATDEDAFARLRGPD
                     IGVKVGEGTTLAAHRVESPTEVANLLGRLLEDRQAWLAGHRAPAIERHSMLSDQRTLA
                     IVDPDARVSWLCHPRADSAAVFGELIGGPTTGYFAVRPLDSNGPSTQRYLPDTMILET
                     KWRSMTVTDYLDVSEHRANELAGRSDLVRVLEGDGRAEILFVPRVDFGRGYTELEITP
                     DGVAVMGGHEPIFLHSPDVDWQITAEGRHQSAHAVVNLGGAPVVLDLRLGRIDHDAVA
                     PLPETDRRRETATFWRAKTEPLRLPSVAPTLVRRSALVLDALRYQPSGAMLAAATTSL
                     PESIGGPRNWDYRFCWPRDSSMSCAS"
BASE COUNT          259 a          548 c          620 g          275 t
ORIGIN      
        1 ctggatgaag gcgctcggca tcgacgccgg tgcagacggt gtgggcagtg caggcggtgt
       61 agacggtgaa cggagcggca gggtgaacgg agcggcaggg tgaacggatg gtccgatgag
      121 ttgagcgagg cactcgccgc ggctgcccgg tcgccgcagt tgctggtggc gtgtgattac
      181 gacggcaccc ttgcccccat cgtggatgac cccgccgcgg cgcaccccat tcgcgcggcg
      241 atgacggcgt tgcgaggcct ggcgctcgtt cccgacacgt cggtgtcggt gatttcgggg
      301 cgagcgctgc gcgacctcgc cttgctcacc cggcttcccg acgaggtcca tctcgtcggg
      361 agtcacggtt cggaattcga accccacgac accggacggt tgggccaggc cgagatagcg
      421 ctgttgcacg atgtgaccag ggagatgctg gcgatcgcat cgaactatcc gggctgctcg
      481 gtcgaaccca agccggtggg ggcggccttt cactaccgcc gcgcggatgc ccatgtcggc
      541 gcgacggccg cggccgaggt cctcgccggg ccggccagac gagcgggcgt tcacgtgcgc
      601 gagggcaagg cggtcgtcga actgtcggtg gtcgacaccg acaagggtgc ggcgctcgac
      661 cggctgcgtt cgatcctcgg tgccgacacg gtgatcttcc tcggagacga cgccaccgac
      721 gaggacgcct tcgcacgcct gcgcggaccc gatatcgggg tgaaggtagg ggaggggaca
      781 acgctggcgg cccaccgggt ggagagcccg accgaggtgg cgaacctgtt gggtcgcctc
      841 ctcgaggacc ggcaggcctg gcttgcaggg caccgtgctc cggcgatcga acgacacagc
      901 atgttgtccg accagcgcac gctggccatc gtcgaccccg acgctcgagt gagttggttg
      961 tgccacccgc gcgccgacag cgcggcggtg ttcggcgagc tcatcggagg gccgaccacc
     1021 gggtacttcg ccgtgcgtcc actcgattcg aacgggccgt cgacgcagcg gtatctcccc
     1081 gacaccatga ttctcgagac caagtggcgc tcgatgaccg tgaccgacta cctcgacgtg
     1141 tcggaacatc gggcgaacga actcgccggg cggtccgatc tggtacgcgt tctcgagggc
     1201 gatgggcgag ccgagatcct gttcgtgccg cgggtcgact tcggtcgcgg ctacaccgaa
     1261 ctcgagatca cccccgatgg ggtggcggtg atgggtggcc acgagccgat cttcctgcac
     1321 agtccggacg tcgactggca gatcaccgcc gaggggcgac atcagagcgc tcatgcggtg
     1381 gtgaacctgg gtggcgcccc ggtggtgttg gaccttcgac tcggtcggat cgaccacgac
     1441 gccgtggcgc cgctgcccga gacggaccgt cggcgcgaga ccgccacgtt ctggagggca
     1501 aagaccgagc cgctgcggct gccctcggtt gccccgacgt tggtgcggcg ctcggcgttg
     1561 gtgctcgatg cacttcgcta tcagccgtcg ggagcgatgc tggccgccgc aaccacgagc
     1621 ctgcccgaga gcatcggcgg gccgcggaac tgggattacc ggttctgttg gccgcgcgat
     1681 tcgtcgatgt cgtgtgccag cc
//
LOCUS       DAOOMA010000611         1700 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_91177_length_1700_cov_3.474772, whole genome shotgun sequence.
ACCESSION   DAOOMA010000611 DAOOMA010000000
VERSION     DAOOMA010000611.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 1700)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1700)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1700
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_91177_length_1700_cov_3.474772"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..589
                     /locus_tag="PLP95_13085"
     CDS             <1..589
                     /locus_tag="PLP95_13085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HPB46784.1"
                     /translation="GLGKALKSTVHPIRNEIPLLLAAEGPKNVALAAEIADGWIPFWF
                     GPSQDAHYRAALGEGFAREDARHGGLGDGFEVVSPLPIIPNDDVEAAADLLRPMLALY
                     IGGMGAKGANFHFDVFARMGFEAECVRIQDLYLDGHKSDAIAAVPLRLVEEVALIGPF
                     DKILAELPAWKETVITTAQINAPVEMLEAVASVLA"
     gene            complement(618..1469)
                     /locus_tag="PLP95_13090"
     CDS             complement(618..1469)
                     /locus_tag="PLP95_13090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB46785.1"
                     /translation="MRKFWQRPQQITSHDLVTTTIRTWKTEDLQSMSWSEPLEWLTRD
                     LQHEAGLTDEARPIVRQRLLDLLLDRAVGGRTGVEAPASGAEPSSETRTVVLAGTDPI
                     AIATLARHYGATAANDPMMTEFCGLEWELDFHLPHFAEWQATDPLAAQYSSAAQRNRF
                     ASNNATVANLATHLWAAFGHLEHLTQIRAAAPRAVIVVLHGGDDSAAANEACRRRAQF
                     CANVERNKVERYWAFRLAAMRDTSNAQLMGFRTAMDPRLIEVNASEALDNPTALAADI
                     AARLVAA"
     gene            1518..>1700
                     /locus_tag="PLP95_13095"
     CDS             1518..>1700
                     /locus_tag="PLP95_13095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HPB46786.1"
                     /translation="MTSDDDLLRFPVRAPKGAGSAYLVRPGDDGAGRGHGVLVLHSWW
                     GLNRAVKDFCNRLCDEG"
BASE COUNT          250 a          492 c          646 g          312 t
ORIGIN      
        1 cggattgggt aaggcgttga agtcgaccgt gcacccgatc cggaacgaaa ttccgttgct
       61 gctggccgcc gagggcccca agaacgtcgc actcgccgca gagattgcgg acggctggat
      121 tccgttctgg ttcggcccga gccaggacgc ccactaccgt gccgcgctcg gcgagggatt
      181 cgcacgcgag gacgcccgtc atggtggcct cggtgacggg ttcgaggtgg tgtcgccgct
      241 gccgatcatt ccgaacgatg acgtcgaggc agccgccgat ctccttcggc cgatgttggc
      301 gctctacatc gggggcatgg gagcgaaggg tgcgaacttc cacttcgatg tcttcgcccg
      361 catgggtttc gaggccgagt gcgtgcggat ccaggacctg tacctcgatg gccacaagtc
      421 cgacgcgatc gcggcggtgc cgctgaggtt ggtggaggag gtggcgctca tcggaccctt
      481 cgacaagatc ctcgcggagc tgccggcgtg gaaggaaacg gtgattacca ccgcccagat
      541 caacgcaccc gtcgagatgc tcgaggcggt tgcgtcggtg ctggcgtagg ggttcgtcgc
      601 cgccgtgcgt cggccgttca ggccgcgacg aggcgggcgg cgatgtcggc tgccagcgcg
      661 gtcgggttgt cgagagcctc ggatgcgttg acctcgatga ggcgcgggtc catcgcggtg
      721 cgaaagccca tcagctgagc gttgctcgtg tcgcgcatgg cagcgaggcg aaatgcccag
      781 taccgctcga ctttgttgcg ctcgacgttc gcgcagaact gagcgcgacg ccgacaggct
      841 tcgttcgctg cagccgagtc gtcgccgccg tggaggacga cgatcacggc gcgaggtgcc
      901 gccgctcgga tctgtgtcag gtgttcgaga tgcccgaacg cagcccacag gtgggtggcg
      961 aggttggcga cggttgcgtt gttggacgcg aaccggttgc gctgtgccgc gctggagtac
     1021 tgcgcggcga gggggtcggt ggcctgccat tcggcgaaat gagggaggtg gaagtcgagt
     1081 tcccactcca gcccacagaa ttcggtcatc atcggatcgt tggcggccgt tgcgccgtag
     1141 tggcgcgcca gggtggcgat agcgatggga tcggttccgg cgagtacgac ggttcgcgtt
     1201 tcggaggagg gttcggctcc cgacgcgggt gcctccaccc cggttcgtcc gccgacagcg
     1261 cggtccaaga ggaggtcgag cagtcgttgg cgcacgatcg gccgcgcctc gtcggtgagg
     1321 cccgcctcgt gctggaggtc gcgggtcagc cattcgagcg gctccgacca ggacatcgac
     1381 tggaggtcct cggtcttcca tgtgcgaatc gtggtggtga cgaggtcgtg ggaggtgatc
     1441 tgttgcgggc gctgccagaa cttgcgcatg gcggcgacgc taccggcgcc ggttcgacgg
     1501 cgtggaacaa tggtcgggtg acgagtgacg acgacctgtt gcggtttccg gtccgtgcgc
     1561 cgaagggtgc ggggtccgcg tatctggtgc ggcccggcga cgatggtgcc gggcggggtc
     1621 acggggtgtt ggtgctgcat tcatggtggg gcctcaaccg ggcggtgaag gacttctgca
     1681 accggctgtg tgacgaaggc
//
LOCUS       DAOOMA010000612         1698 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_91319_length_1698_cov_3.989044, whole genome shotgun sequence.
ACCESSION   DAOOMA010000612 DAOOMA010000000
VERSION     DAOOMA010000612.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 1698)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1698)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1698
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_91319_length_1698_cov_3.989044"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..335)
                     /locus_tag="PLP95_13100"
     CDS             complement(<1..335)
                     /locus_tag="PLP95_13100"
                     /inference="COORDINATES: protein motif:HMM:NF020897.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HPB46787.1"
                     /translation="MEQILSGIGVLDKAMYLVELLERRSRTLAELVEDSAMSRSTVHR
                     MLLALELHGLAARDADGRWHLGARLIGLGVAASDSFPLARLAEPVLARLRDESGESVQ
                     LYVRQGEHRV"
     gene            412..>1698
                     /gene="leuC"
                     /locus_tag="PLP95_13105"
     CDS             412..>1698
                     /gene="leuC"
                     /locus_tag="PLP95_13105"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012230463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HPB46788.1"
                     /translation="MSQPKTLSQKVWDRHVVRQVDGEPDLLFIDLHLVHEVTSPQAFD
                     GLRLNHRTPRHPELTVAIEDHNIPTANIDQPIKDPISAKQLQVLGKNTDEFDITHYPM
                     GHKYNGIVHVVGPEQGLTLPGMTVVCGDSHTATHGAFGALAFGIGTSEVEHVLATQTL
                     RQPELGTLAVTVNGELPEGSTAKDIILAILAELGTSGGIGYIAEYRGSAIEALSMEGR
                     MTICNMSIEAGAKAGLIAPDEVTFEYLKGRPHAPQGELWDRAVEEWKTLKTDEGAVFD
                     KEIVLDASKIFPHVSWGTNPGQVIQMTDVIPSPEDFSDPVERNAAERALEYMDLTPGQ
                     PIKEVPVDVVFIGSCTNSRIEDMRAAAAVAKGHKVNPAVTTYVVPGSHLVKAQAEAEG
                     LDKIFIEAGFDWREPGCSMCLAMNPDKLTPGQRSAST"
BASE COUNT          339 a          608 c          502 g          249 t
ORIGIN      
        1 acccggtgct ccccctggcg cacgtagagc tgcacggatt cgcccgactc gtctcgcagc
       61 cgagccagca ccggttcggc gagacgggcg aggggaaacg agtccgacgc tgcgacgccc
      121 aggccgatca ggcgcgcccc gaggtgccaa cggccgtcgg cgtctcgtgc ggcgagcccg
      181 tgcagttcga gcgccagcaa catgcggtgg acggtggaac gcgacatcgc cgagtcctcg
      241 acgagctcgg cgagggtccg ggagcgacgc tcgagcagct cgacgaggta catcgccttg
      301 tcgagcactc cgattccgct tagtatctgt tccatcagtt gagattctga tcccacatat
      361 tgggacctgt caagacactg acgcccaacc accccacaag gaagtcccca gatgagccag
      421 cccaagacgc tgagtcagaa ggtctgggac cgccacgtcg tccgccaggt cgacggcgaa
      481 cccgacctgt tgttcatcga cctgcacctg gtgcacgagg tcacgagtcc ccaggccttc
      541 gacggtctgc gcctcaacca ccgaacgccg cgccaccccg aactcaccgt ggcgatcgag
      601 gaccacaaca tccccaccgc caacatcgat cagcccatca aggacccgat ctcggccaag
      661 cagcttcagg tgctcggcaa gaacaccgac gagttcgaca tcacccacta cccgatgggc
      721 cacaagtaca acggcatcgt ccacgtcgtg ggccccgaac aaggcctcac gctgcccggc
      781 atgaccgtcg tgtgcggcga cagccacacc gccacccacg gagcgttcgg cgccctggcg
      841 ttcggcatcg gcaccagcga ggtcgaacat gtcctcgcca cccagacgct gcgtcagccc
      901 gaactcggca ccctggcggt cacggtgaac ggcgaacttc ccgagggctc gaccgccaag
      961 gacatcatct tggccatcct cgccgaactc ggcaccagcg gcggcatcgg ctacatcgcc
     1021 gagtaccgcg gttcggccat cgaggcactg tcgatggaag gtcgcatgac catctgcaac
     1081 atgagcatcg aagcgggggc caaggccggt ctcatcgccc ccgacgaggt caccttcgag
     1141 tacctcaagg gacgcccgca cgcaccccag ggtgagctgt gggaccgggc ggtcgaagag
     1201 tggaagacgc tcaagaccga cgagggcgct gtgttcgaca aggagatcgt gctcgacgca
     1261 tcgaagattt tcccgcacgt ctcctggggc accaacccgg gccaggtcat ccagatgacc
     1321 gatgtcatcc cctcccccga ggacttcagc gatccggtcg aacgcaacgc ggctgagcgc
     1381 gcgcttgagt acatggacct tacgcccggt cagccgatca aagaggtacc ggtcgacgtc
     1441 gtcttcatcg gctcctgcac caactcgcgc atcgaagaca tgcgtgccgc ggccgccgtc
     1501 gccaagggcc acaaggtcaa cccggccgtc accacctacg tggttccggg ctcgcacctc
     1561 gtgaaggcac aggccgaggc cgaggggctc gacaaaatct tcatcgaggc cggtttcgac
     1621 tggcgtgaac cgggatgctc catgtgtctc gccatgaacc ccgacaagct cacccccggc
     1681 cagcgctccg cctcgacc
//
LOCUS       DAOOMA010000613         1692 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_91779_length_1692_cov_4.467318, whole genome shotgun sequence.
ACCESSION   DAOOMA010000613 DAOOMA010000000
VERSION     DAOOMA010000613.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 1692)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1692)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1692
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_91779_length_1692_cov_4.467318"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1072)
                     /locus_tag="PLP95_13110"
     CDS             complement(<1..1072)
                     /locus_tag="PLP95_13110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HPB46789.1"
                     /translation="MRDAGPLLGGLDDLGPVGDASGEQPAMWSPVDPRRRELLDVLHV
                     AVRIVGASSGRIVDGAGIGDFASTDLASTDCVPAVRHELAHELRIALLHPTSGDRIGD
                     MVLHDSEVPSDGDLAVLRLLGTLAAGTIAGVDRYRELRRGRVLGLVVLDAQGRLRSAS
                     PDLAMAFGVDLDRSPDVAGLDLVHPDDLETAMAGLAKTTSFPGEKFPLDVRLRLGDGS
                     WLPVEMTALSSETETIDGVVLQVRDVVDRTAVDMLVSHQSLVLSMIGRGEQLATSLGE
                     LARLVAQQVGGGCVITLRRRGESAVAGDDSSEVVAAFGVSEAFVEAVDATAVDADSTP
                     WGTATFRAQSTRVDDLFVEPCGE"
     gene            1272..>1692
                     /locus_tag="PLP95_13115"
     CDS             1272..>1692
                     /locus_tag="PLP95_13115"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HPB46790.1"
                     /translation="MTATIPFPPDATSHDIAVVELSKSYGPVDVFTKISFSASPGHRL
                     GLVGENGAGKSTLLRMIVGTTDRDGGTIRRPSPVGYLEQELPYPDTATIDDVVEETLA
                     PIREIEAALTTAALDLAYVDAAIATAAQQTYDDALEAA"
BASE COUNT          335 a          616 c          494 g          247 t
ORIGIN      
        1 attcgccgca cggttcgacg aacaggtcgt ccacccgggt cgactgggcg cgaaaggttg
       61 cggttcccca cggagtcgag tcggcatcga ccgccgttgc gtcgaccgcc tcgacgaacg
      121 cctcgctgac accgaacgcg gcgaccacct cactcgaatc gtcgccggcc actgcgctct
      181 cgccgcgtcg gcgcaacgtg atgacacagc cgcccccgac ctgttgcgca accaggcgag
      241 cgagttcacc gagggaggtg gccaactgct cgccacgacc gatcatcgac aacaccaacg
      301 actgatgcga caccaacatg tccacggcgg ttcgatcgac cacatctcgg acctgcaaca
      361 cgacgccgtc gatcgtctcg gtttcgctcg acagcgcggt catctcgacc ggcagccacg
      421 aaccgtcacc gaggcgcaac cgcacgtcga gggggaactt ctctcccgga aacgacgtcg
      481 tcttggccag tccagccatc gcggtctcga ggtcatcggg gtgcacgagg tcgaggccgg
      541 cgacgtcggg ggaacggtcg aggtcgacgc cgaacgccat cgccagatcg ggagatgctg
      601 aacgcaaacg gccctgggca tcgagcacca ccaagccgag cacccggccg cgtcgaagct
      661 cgcgatagcg gtccacaccg gcgatggttc ctgccgccaa cgtgcccagc agtcgcagga
      721 ccgcaagatc accgtcggag ggaacttcgc tgtcgtgcag gaccatgtcg ccgatccgat
      781 cgcccgacgt cgggtgcagc aacgcaatcc gcaactcatg agcgagttcg tgacgcaccg
      841 ccggcacaca gtcggtcgag gcaagatcgg tcgaggcaaa gtcgccgatc cccgcaccgt
      901 cgacgattcg gcccgaactc gcgccgacga ttcgaaccgc gacgtgcaac acgtcgagga
      961 gctcgcgccg tcgaggatcg accggcgacc acatcgccgg ctgttcaccg gaggcatcgc
     1021 caaccggtcc caaatcgtcg agcccgccaa ggagcggacc agcatcccgc actctcttat
     1081 tgtccaccat tgacttcgtt atcgacccgt cgagccccga acctgaactt gttctccgat
     1141 aaccagtggt cgtgatcacg accactggtt atcgggaaac aggctggggg gcgcggtcgc
     1201 tagagtcgcg gcatggagat ctgagcgata cccgacgcct caaccgaatt cgtacagatc
     1261 tggagggtct catgaccgcg accatcccct ttccgcctga cgccacctca cacgacatcg
     1321 ccgtcgtaga actgtcgaag tcctacggcc ccgtcgacgt cttcaccaag atcagcttct
     1381 ccgcatcgcc cgggcaccgc ctcggcctcg tcggcgagaa cggcgccggc aagtcgacgt
     1441 tgttacgcat gatcgtgggc accaccgacc gcgacggcgg caccattcgc cgacccagcc
     1501 ccgtcggcta cctcgaacag gaactcccct atcccgacac cgcgaccatc gacgacgtcg
     1561 tcgaggagac cctcgcaccc atacgcgaga tcgaggccgc gctcacgacc gccgcgctcg
     1621 acctggccta cgtggacgcc gccatcgcga ctgcggcaca gcagacctac gacgacgcgc
     1681 tcgaagccgc gc
//
LOCUS       DAOOMA010000614         1692 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_91787_length_1692_cov_3.877825, whole genome shotgun sequence.
ACCESSION   DAOOMA010000614 DAOOMA010000000
VERSION     DAOOMA010000614.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 1692)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1692)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1692
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_91787_length_1692_cov_3.877825"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            45..755
                     /locus_tag="PLP95_13120"
     CDS             45..755
                     /locus_tag="PLP95_13120"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HPB46791.1"
                     /translation="MIRLQKVLAQAGIGSRRVCEEYIEDGRVSVNGAIAELGDKVDPD
                     VDVVEFDGVQIGVRPGMMYYLLNKPTGVVTTASDPQGRPTVVDLVPQEPRVFPVGRLD
                     FETSGLLLLTNDGDLTHRLTHPSFGVDKEYLAEVEGVPKRSALRALREGVELDDGPTA
                     PASVSQPAENLLKLTIHEGRNRQVRRMCEAVGHPVVRLVRTRIGPLTDTTLKAGQWRE
                     LTQDEVRALERAVRDPDD"
     gene            855..1223
                     /gene="aroH"
                     /locus_tag="PLP95_13125"
     CDS             855..1223
                     /gene="aroH"
                     /locus_tag="PLP95_13125"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015798765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004106 - chorismate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HPB46792.1"
                     /translation="MSISVRALRGATTFEVDEADHVSHRVVELVSSMLERNGVHHDDL
                     VSIIFTATDDLTSTFPATAARQLGLGDVPLICARELSIAGAMPRCVRVLMHLETDRSR
                     SELRHVYLHDARALRDDLPE"
     gene            1234..>1692
                     /locus_tag="PLP95_13130"
     CDS             1234..>1692
                     /locus_tag="PLP95_13130"
                     /inference="COORDINATES: protein motif:HMM:NF014234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004665 - prephenate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0008977 - prephenate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0006571 - tyrosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase/arogenate dehydrogenase
                     family protein"
                     /protein_id="HPB46793.1"
                     /translation="MAEPEAPQVTRSARRAQLIGTGLIGGSVGLVLKAQGWHVTGFDA
                     DESAASLAIELGAIDEIGLDLNAEVCFVALPVGSVAAAANRLLDMPGTNWVVTDVGSV
                     KAPIAAAVAHPRFVGGHPMAGSEVEGVAGSRADMFDGAVWVLTPSEQTDPL"
BASE COUNT          281 a          545 c          586 g          280 t
ORIGIN      
        1 gaactcgatg cgctcgacat cttgaaagac gacgaggccg agggatgatc cgactccaga
       61 aggtgctcgc ccaggcgggt atcgggagcc gacgggtgtg cgaggaatac atcgaggacg
      121 gtcgcgtgtc ggtcaacggt gcgatcgccg agcttggaga caaggtcgat ccggacgttg
      181 acgtcgtcga gttcgatggc gtgcagatcg gcgttcggcc gggaatgatg tattacctgc
      241 tcaacaagcc gaccggcgtg gtgacgacgg cttcggatcc ccaggggcgg ccgacggtgg
      301 tcgacctggt tccgcaggag ccccgcgtct ttcccgtggg tcgcctcgat ttcgagacct
      361 cggggttgtt gctgttgacc aacgacggag atctcaccca ccgcctgacc cacccgagct
      421 tcggcgtgga caaggagtat ctcgccgagg tcgaaggggt gccgaaacgc tcggcgttgc
      481 gggccctgcg cgagggggtc gaactcgacg acgggcccac cgctcccgca tcggtgtccc
      541 agccggccga gaacctgttg aagctcacga tccatgaggg gcgtaaccgt caggttcgac
      601 gcatgtgcga ggcggtcgga catccggtgg tgcgcctcgt tcgaacccgg atcggcccgc
      661 tcaccgacac gaccctcaag gctgggcagt ggcgcgaact cacccaggac gaggtgcggg
      721 ccctcgaacg ggccgtgcgc gatcccgacg actagtgctg gtgggcctct cccggagggc
      781 cgctccggac ggtcgcaccg gaaggtcgcg ccagcgggag ggtgacgctg cgcggccaac
      841 tacaatcgct ggccatgtcg atcagcgtcc gtgccctccg aggcgccacc acctttgagg
      901 tcgatgaggc cgaccacgtg tcacaccgag tggtcgaact cgtgtcgtcc atgctggagc
      961 gaaacggcgt tcaccacgac gatctcgtgt cgatcatctt caccgcgacc gacgatctca
     1021 ccagcacgtt cccggcgacc gcagcacgcc aactcggcct cggcgacgtg ccgctgatct
     1081 gtgcaaggga actgtcgatc gctggcgcga tgccgcgttg tgtgcgggtg ctcatgcacc
     1141 tcgaaaccga caggtcccgg tccgagctgc gccatgtgta cctccacgac gcacgggcgc
     1201 tgcgcgacga ccttcccgaa tagatgcggc gccgtggctg aaccagaagc accgcaggta
     1261 acccgctcgg cgcgccgtgc ccagctgatc ggcaccgggc tgatcggcgg ttcggtcggt
     1321 ctcgtgttga aagcgcaggg gtggcacgtc accggtttcg acgccgacga atccgcagcg
     1381 tccctggcca tcgagctcgg agcgatcgac gagatcggcc tcgacctcaa cgccgaggtg
     1441 tgcttcgtcg ccctacccgt cggttcggtc gccgccgcgg ccaatcggct gctcgacatg
     1501 ccgggaacga actgggtggt gaccgacgtg gggtcggtga aggccccgat cgcagcggcg
     1561 gtggcccacc cgaggttcgt cggtgggcac ccgatggcgg gatccgaggt cgagggggtc
     1621 gccggatcgc gagcggacat gttcgacggt gcggtctggg tgttgactcc ttcggaacag
     1681 accgatccgc tc
//
LOCUS       DAOOMA010000615         1685 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_92347_length_1685_cov_3.226994, whole genome shotgun sequence.
ACCESSION   DAOOMA010000615 DAOOMA010000000
VERSION     DAOOMA010000615.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 1685)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1685)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1685
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_92347_length_1685_cov_3.226994"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..737
                     /locus_tag="PLP95_13135"
     CDS             <1..737
                     /locus_tag="PLP95_13135"
                     /inference="COORDINATES: protein motif:HMM:NF012697.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HPB46794.1"
                     /translation="VELIDLVAARSEARGRRPDLVGVTGSVAVGKSSMAAELAAALQA
                     RYRLTVEVVSTDGFLLPNATLEPLGRAFHKGFPDTYDTAGLIDFLETIRSGAEATFAL
                     YDHIRYDLSAERGRIENPDMVIVEGLNLFGSSAVAGDRRVAECLDERIYVDADTSVVA
                     TWFVDRFMKFRDQASSNPDSFYSMFVDMSDSDAVATAELVWDEINGVNLREHIAPARS
                     EATIIVTKADDHSIESIEIAELGFPI"
     gene            1069..>1685
                     /locus_tag="PLP95_13140"
     CDS             1069..>1685
                     /locus_tag="PLP95_13140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB46795.1"
                     /translation="MTMFRRLSTTRSGITGATARGLSILVFVGLSTLAVSCSEVSEAS
                     SFPEPDRATDLFEITRPNREITTLRAVDERSILVEATDARSTGSNARSVFAWNNAMAG
                     SSQWSCATVRHEGGGTQEGVALRIRTDDARVRAITVTKNIMFGATWVYNVHLWDTARR
                     GAVPATLLTSVDLRRRFGPVDSVDTPRRLCAAVEGNELRFKAWRAS"
BASE COUNT          290 a          548 c          535 g          312 t
ORIGIN      
        1 ttgtggagct catcgacctg gtcgccgctc ggtccgaggc acgcggccgc cgccccgatc
       61 tcgtcggcgt gacaggttcg gtcgccgtcg ggaaatcgtc gatggccgcc gagctcgcag
      121 ccgcacttca agctcgatac cgcctcaccg tcgaggtcgt gtcgaccgac ggattcctcc
      181 tccccaacgc gacgctcgaa ccgctcggac gggcctttca caagggcttc cccgacacct
      241 acgacaccgc ggggctcatc gactttctcg agacgatccg gtccggcgcc gaagcgacct
      301 ttgcgctgta tgaccacatt cgctacgacc tgtcggcgga acgcggacgc atcgagaacc
      361 ccgacatggt catcgtcgaa gggctgaacc tgtttgggtc gagtgcggtc gccggagacc
      421 gtcgagtcgc ggagtgcctc gacgaacgca tctacgtcga cgccgacacg tcagtcgtcg
      481 ccacatggtt cgtcgaccgg ttcatgaagt tccgcgacca ggcgtcatcc aacccggaca
      541 gcttctattc gatgttcgtg gacatgagcg attcagatgc cgtagcgact gccgagctcg
      601 tgtgggacga gatcaacggt gtcaatcttc gtgagcacat cgcccccgct cgatcagagg
      661 ccacgatcat cgtgaccaag gcggatgatc actcgatcga gtcgatcgag atcgccgagc
      721 tcggcttccc catctgaggg cctggccgaa aacacgacga aggcccgggg ggatgggcct
      781 tcgtcgttgc gtcagctatc gctttcgctc gttctcgacg atcggagggg gggggagatg
      841 gtcgagaacg ttcgatggcc cgtaggccag tactctcgac agttgcgccg agagggcgag
      901 cgaaccgggg ggatggtccg cttcgcaacg ccactcacca tagcttcgct cactccagtt
      961 agcatccgat ttggcgtcgt tatggcgctt ttcacacatt gccgatgccg actgtcgccc
     1021 gaccccgtaa actcatcggt ccatacaggc ggctcccaag gcggtgacgt gaccatgttc
     1081 cggcgtttga gcacgacgcg atccggtatc acgggggcca ccgcgcgcgg gttgtcgatc
     1141 ctggtgttcg tgggcctgtc gacgctggcg gtgagctgca gcgaggtgtc ggaagcatcc
     1201 agcttcccgg aacccgatcg agcgaccgac ttgttcgaga tcacccgccc gaaccgtgag
     1261 atcaccaccc tgcgcgcggt cgacgagcgt tcgattctcg tggaggccac cgacgcaaga
     1321 tcgacggggt cgaacgcccg aagcgtgttc gcgtggaaca acgccatggc cggttcctcc
     1381 cagtggtcgt gcgccaccgt tcgccacgag ggtggtggca cccaggaagg ggtggcgttg
     1441 cgcatccgca ccgatgacgc tcgggtacga gccatcaccg tgaccaagaa catcatgttc
     1501 ggggcgacgt gggtctacaa cgttcacctc tgggacaccg ctcgccgcgg cgcggtgccc
     1561 gcgacgctgc tgacctcggt ggatctccgt cgccgattcg gacccgtcga ctccgtcgat
     1621 accccccgac ggctgtgtgc ggcggtcgag ggcaacgagt tgcgtttcaa ggcctggcgt
     1681 gcgtc
//
LOCUS       DAOOMA010000616         1681 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_92703_length_1681_cov_3.406519, whole genome shotgun sequence.
ACCESSION   DAOOMA010000616 DAOOMA010000000
VERSION     DAOOMA010000616.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 1681)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1681)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1681
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_92703_length_1681_cov_3.406519"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..604)
                     /locus_tag="PLP95_13145"
     CDS             complement(<1..604)
                     /locus_tag="PLP95_13145"
                     /inference="COORDINATES: protein motif:HMM:NF014704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HPB46796.1"
                     /translation="MPGRVNALDVALVLFALSAVFGGWRAGFVHRAFSWLGLFGGILV
                     ASIVLRRIASATEVATDAPALLRSLAVLVVGAVLGNAVGTWLGSRASAAVDESAFDLL
                     DSAGGALLGLASTALMAWMVLPTMTMVPGWPADIARSSVVASRLNRALGPAPDVLDGV
                     SQSLGLGAFDGLWHLMEQIDIGGLQPEAPLETPLSTEVTDS"
     gene            complement(589..1365)
                     /locus_tag="PLP95_13150"
     CDS             complement(589..1365)
                     /locus_tag="PLP95_13150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012228721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine-N1)-methyltransferase"
                     /protein_id="HPB46797.1"
                     /translation="MSASELHEGDVVVLLDNKHRRYLLTLQSGKEFHSHAGYIAHDEI
                     IGAGEGAVLTSTRGATYTVLRPTLSEFILKMPRGAQVIYPKDIGPILMMADIGPGMRV
                     LESGVGSGALSMGMLRTGVHITGYELREDFAERAQANVRKFLGEEALERYDVQLRNCY
                     EGIDERDLDRVVLDLPEPWQVVPHARTALRPGGIIVAYSPSIVQVMQFRDALERDGFI
                     LAETLEVLNRGWYIEGQAVRPDHRMVAHTGFLTSARCPGG"
     gene            complement(1362..>1681)
                     /locus_tag="PLP95_13155"
     CDS             complement(1362..>1681)
                     /locus_tag="PLP95_13155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020379538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tRNA(Ile)-lysidine synthetase"
                     /protein_id="HPB46798.1"
                     /translation="YCVVSGIDYVIDECPMAAGNKHIGYKNLLNDLERQSPGTKSDFY
                     LSFLRRASALFTPEADEQREALGTCATCGSPSPSEVCAFCRLVERAGGAQPVELTTKD
                     HRE"
BASE COUNT          302 a          563 c          550 g          266 t
ORIGIN      
        1 ccgagtcggt gacctcggtc gacagcggcg tctcgagcgg cgcctcgggc tgcagtcccc
       61 cgatatcgat ctgctccatg aggtgccaca gcccgtcgaa ggcgccgagg ccgagggatt
      121 gagacacacc gtcgagcaca tcgggggccg ggccgagtgc gcgattgagt cgactcgcca
      181 ccaccgacga gcgggcgatg tctgcgggcc atcccggaac catcgtcatc gtcggaagca
      241 ccatccacgc catcaacgcg gtcgacgcca ggccgagcag cgcacctccg gccgaatcga
      301 gcaggtcgaa agccgactcg tcgaccgcag cgctggcgcg ggaccccaac caggtcccca
      361 ccgcgttgcc gagaaccgca ccgacgacca gaaccgcgag cgagcgcaac agtgccgggg
      421 catcggtggc cacctcggtc gcgctggcga ttcggcgcaa cacgatcgac gcgacgagga
      481 tgccgccgaa gagcccgagc cacgaaaacg cccgatgcac gaacccggcg cgccaaccac
      541 cgaacaccgc cgaaagggcg aacaggacca gcgcgacgtc gagggcgttc accctcccgg
      601 gcacctcgcc gaggtgagga accctgtgtg agccaccatg cgatgatcgg ggcgcaccgc
      661 ctggccctcg atgtaccacc cgcggttgag cacttcgagg gtctccgcca ggatgaatcc
      721 gtcgcgttcg agggcgtcgc gaaactgcat cacctgcacg atgctcggcg agtacgcaac
      781 gatgatgccc ccgggccgca acgcggttcg tgcgtggggc accacctgcc acggttcggg
      841 caggtccaac accacgcggt cgaggtcgcg ttcatcgatg ccctcgtagc agttgcgaag
      901 ctgcacgtca tagcgttcga gggcctcctc gccgaggaac ttgcgtacgt tcgcctgggc
      961 gcgttcggcg aagtcctctc gaagctcgta tccggtgatg tgcacgccgg tgcgcaacat
     1021 gcccatcgac agcgcacccg atcccacccc cgattcgagt acccgcatgc ccggtccgat
     1081 gtcggccatc atcaggatcg gcccgatgtc tttgggatag atgacctgcg ctccgcgtgg
     1141 catcttgaga atgaactcgc tcagcgtcgg gcgcagcacc gtgtaggtgg cgcctcgcgt
     1201 cgaggtcagc acggcaccct ccccggctcc gatgatctcg tcgtgggcga tgtaaccggc
     1261 gtgagaatga aactccttgc cggattgcag ggtgaggagg tagcggcggt gcttgttgtc
     1321 caacaacacc accacgtcgc cttcatggag ttccgatgca ctcactcgcg gtgatccttc
     1381 gtggttagtt cgacgggttg tgcgccgccc gcacgttcga cgaggcggca aaaggcgcag
     1441 acttccgagg ggctcggcga tccgcacgtc gcacaggtcc cgagggcttc gcgttgctcg
     1501 tccgcctcgg gcgtgaacag cgcggaggcg cgacgcagaa agctcaggta gaagtccgac
     1561 ttggtgccgg ggctctggcg ctcgagatcg ttgagcaggt tcttgtagcc gatgtgtttg
     1621 ttgccggccg ccatcgggca ctcgtcgatg acgtagtcga tgccgctgac gacgcagtag
     1681 g
//
LOCUS       DAOOMA010000617         1677 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_93040_length_1677_cov_3.987053, whole genome shotgun sequence.
ACCESSION   DAOOMA010000617 DAOOMA010000000
VERSION     DAOOMA010000617.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 1677)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1677)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1677
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_93040_length_1677_cov_3.987053"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..373)
                     /locus_tag="PLP95_13160"
     CDS             complement(<1..373)
                     /locus_tag="PLP95_13160"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HPB46799.1"
                     /translation="MAGPGRPRRFDEAHERQLLLDASIAVMERNGFDDVAVADILAEA
                     DVSTRSFYRHFASKDELVCAIYHREAQRIAEQITLRTKNAADPVEALDAWLSAMLAVG
                     HHPRKAKRAAILASPGARRAEG"
     gene            462..1118
                     /gene="nth"
                     /locus_tag="PLP95_13165"
     CDS             462..1118
                     /gene="nth"
                     /locus_tag="PLP95_13165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HPB46800.1"
                     /translation="MKRRDKADRIGEMLDEMYPEQPIPLDHHDPYTLLVAVALSAQTT
                     DKKVNEVTPALFARASTPQEMALLDPEEIREIIREVGLAPTKAKNLSTMAHQIVEHGG
                     EVVADWEFLESLAGVGHKTASVVMSQAFDVPAFAVDTHIHRLAHRWGLSNGTTVDRTE
                     RDLKAVFPKDTWNRRHLQIIFFGREHCPARNHDLTTCPICSWASTKKMQSGAVTHSKR
                     "
     gene            complement(1163..>1677)
                     /locus_tag="PLP95_13170"
     CDS             complement(1163..>1677)
                     /locus_tag="PLP95_13170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB46801.1"
                     /translation="VGVVDDEQIGIGPIRGRDHRGRPLALDERAVSYEIAEVVQRHLG
                     GATPSTTRAAIESLCARVPNEPNLRRFAAQLALMCGDPGRAVTHVDHALTLEGTPGAR
                     AKLLLARSRAHHSLGDPVAAATDRDEVIALGRDSAPFEFAAAHREQAKALSASALRKL
                     SIDILHLDAR"
BASE COUNT          292 a          534 c          587 g          264 t
ORIGIN      
        1 acccctcggc gcgtcgagcc cccggcgacg cgagaatggc ggctcgtttg gccttgcgag
       61 gatggtggcc gaccgccagc atcgcggaga gccaggcatc gagggcctcg accggatcgg
      121 cggcgttctt ggtccgcagg gtgatctgct cggcgatgcg ctgagcctca cgatgataga
      181 tggcgcagac cagttcgtcc ttggacgcga aatgtcgata gaaggagcgg gtcgacacgt
      241 cggcctcggc gaggatgtcg gcgaccgcca catcgtcgaa tccgttgcgt tccatgacgg
      301 cgatcgacgc atccaacaga agctgtcgtt cgtgtgcttc gtcgaaacgc ctcgggcgcc
      361 cgggaccggc catggcgccc aacgtatcgg acaacgttcg ggccgtgacg ctcgatcgct
      421 accctttggc gacgtaaccg ttggagagtg gagcgagggt cgtgaaacgc cgggacaagg
      481 ccgaccgcat cggcgagatg ctcgacgaga tgtaccccga acaaccgatc ccgctcgacc
      541 accacgatcc ctacaccctg ctcgtggccg tggcgctgtc ggcacagaca accgacaaga
      601 aggtcaacga ggtgaccccg gcgctgttcg cgagggcatc gacccctcag gagatggcgt
      661 tgctcgaccc cgaggagatc cgcgagatca tccgcgaggt cggcctcgcg ccgacgaagg
      721 ccaagaacct gtcgacgatg gcacatcaga tcgtcgaaca cgggggcgag gtcgtcgcgg
      781 attgggagtt cctcgaatcg ctggccgggg tcgggcacaa gaccgcgagt gtggtgatga
      841 gccaggcctt cgacgtcccg gccttcgccg tcgacacgca catccatcgg ctcgctcatc
      901 ggtgggggct gtcgaacgga accacggtgg atcgcaccga acgcgatctc aaggcggtgt
      961 ttcccaagga cacctggaac cgccgtcacc tgcagatcat cttcttcggg cgcgagcact
     1021 gcccggcgcg gaaccacgat ctgacgacct gtccgatctg ttcgtgggcg tcgacgaaga
     1081 agatgcagtc gggcgcggtc acacactcga aacgctgaat cgagatgcgc tgaatcgcag
     1141 cgcgcggcga cgttgcctcg gctcagcggg cgtcgaggtg caagatgtcg atcgagagct
     1201 tgcgcagcgc cgaggccgac agcgctttgg cctgctcgcg atgggctgcg gcgaactcga
     1261 agggcgccga gtcccggccg agggcgatca cctcgtcgcg gtcggtcgcc gcggcgaccg
     1321 ggtcgccgag tgaatggtgg gctcgtgagc gggcgaggag cagcttggct cgtgcgccgg
     1381 gggtgccctc gagggtgagc gcgtggtcga catgggtgac cgctcggccg gggtcgccgc
     1441 acatcagcgc gagctgggcg gcgaaacgac gcaggttcgg ctcgttcgga acccgcgcac
     1501 acagcgactc gatcgcggcc cgtgtcgtcg agggagttgc cccgccgagg tgccgctgga
     1561 cgacctccgc gatctcgtag gagaccgccc gttcgtcaag agcgagcggt ctgccgcggt
     1621 ggtcgcgacc ccgaatcggt ccgatgccga tctgttcgtc gtcgacgact cccacga
//
LOCUS       DAOOMA010000618         1677 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_93046_length_1677_cov_3.743527, whole genome shotgun sequence.
ACCESSION   DAOOMA010000618 DAOOMA010000000
VERSION     DAOOMA010000618.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 1677)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1677)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1677
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_93046_length_1677_cov_3.743527"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1383)
                     /gene="gltX"
                     /locus_tag="PLP95_13175"
     CDS             complement(<1..1383)
                     /gene="gltX"
                     /locus_tag="PLP95_13175"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HPB46802.1"
                     /translation="MSSPYRLRFAPSPTGYLHLGSARAALFNWLHARHLAAQGHEAEF
                     VLRIEDTDRERSQPELIQVIFDVLEWLGIDYDGEATFQSDRADLHAAAIARLVESGAA
                     YRCDCSQDEVAARNAAVGGGASGYDGFCRDRQVSAEGPHVVRFRTPDEGATSWDDLIR
                     GTITVEHASIEDFVLARTDGTPVFLVANAVDDGDMGITHVIRGEDLINVTPKVLLLRE
                     ALGYEGRPEFAHMPLIVNEQRKKLSKRRDDVSVEDYKRRGFLPEAMVNYLALLGWGPS
                     DGIEVRPISEIIELFDISDVGSSPAMFDVKKLEAINGEYIRALDVDEFVARCEPWFAQ
                     QPWAERYDADVFARIAAEVQLRARVLSEVPPQVDFLFLDEISIDEASWDKALKGVDDA
                     AAWLDEVLGRYENLGEWSAEALHATTLEMAEANGLKLGKAQGPIRVAVTGRTVGPPLF
                     EALAVLGRDET"
     assembly_gap    1421..1430
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1479..>1677
                     /locus_tag="PLP95_13180"
     CDS             1479..>1677
                     /locus_tag="PLP95_13180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB46803.1"
                     /translation="MTRRSRNFIAFLAAGVAVTGLVVAFTLGEPTDATTPDTTVAPTT
                     APTTAATTTVPIHLEPAVTEIA"
BASE COUNT          292 a          602 c          508 g          265 t
ORIGIN      
        1 ggtctcatca cgtccgagta cggcgagcgc ctcgaacagc ggcggcccga ccgtacggcc
       61 ggtgaccgct acccgaatcg ggccctgggc cttaccgagt ttcagcccgt tcgcctcggc
      121 catttccaac gtcgtcgcat gcaacgcctc ggcgctccac tcccccaggt tctcgtaccg
      181 cccgagcacc tcatcgagcc acgctgcggc gtcgtcgacc cccttgagcg ccttgtccca
      241 cgaggcctcg tcgatggaaa tctcgtcgag gaacaagaaa tcgacctgcg gcggcacctc
      301 cgacaacacc cgcgcccgaa gctgcacctc cgcggcgatg cgagcgaaca cgtccgcgtc
      361 gtatcgctcc gcccacggct gctgtgcgaa ccacggctcg caacgggcga cgaactcgtc
      421 gacgtcgagc gcacggatgt actcgccgtt gatcgcctcg agcttcttca cgtcgaacat
      481 cgccggcgac gaaccgacgt cggagatatc gaacaactcg atgatctcgg aaatcgggcg
      541 aacctcgatg ccatcggagg gcccccagcc cagcagcgcc aggtagttga ccatcgcttc
      601 gggtaggaaa ccgcggcgct tgtagtcctc gacgctcacg tcgtcgcgtc gcttcgacag
      661 ctttttgcgc tgctcgttga cgatgagcgg catgtgtgcg aactcgggcc gcccctcata
      721 gcccaacgcc tcgcgcaaca acagcacctt cggcgtgacg tttatgaggt cctcgccgcg
      781 gatgacatga gtgatgccca tgtcaccgtc atcgaccgcg ttggccacga gaaacaccgg
      841 ggtgccgtcg gtgcgggcca acacgaagtc ctcgatgctg gcgtgttcga cggtgatggt
      901 gccgcggatc agatcgtccc agctcgtcgc cccctcgtct ggggtacgga accgcacgac
      961 gtgaggccct tcggccgaca cctgacggtc gcggcaaaag ccgtcgtacc ccgatgcccc
     1021 accgccgacc gcggcgttgc gcgccgcgac ctcgtcctgg ctgcagtcgc agcggtacgc
     1081 cgcgccggac tcgaccaacc gtgcgatcgc cgcggcgtgc aggtcggcgc ggtcggactg
     1141 gaaggtcgcc tcgccgtcgt agtcgatgcc gagccactcg agcacgtcga agatgacctg
     1201 gatgagttcg ggctggctcc gctcgcggtc ggtgtcctcg atccgcagca caaactccgc
     1261 ctcgtgcccc tgagcggcga gatggcgggc gtgcaaccag ttgaacagtg cggcccgagc
     1321 gctgccgaga tgcaggtaac cggtcggaga cggagcgaaa cgaaggcgat agggagaact
     1381 cacggcccaa ggctaccgcc gacacccacc cggctccgga nnnnnnnnnn agcccgccac
     1441 ggtcgaaacc aggtcgtctc cctagactga acggtgtcat gacgcgtcgt tcacgtaatt
     1501 tcatcgcgtt tctcgccgcc ggggtcgccg ttaccgggct cgtcgtggcc ttcaccctcg
     1561 gcgaaccgac cgatgccacg acaccggaca cgacggtggc gccgacgacc gccccgacca
     1621 ccgccgccac gacaaccgtt ccgatccacc tcgagccggc ggtcaccgag atcgcca
//
LOCUS       DAOOMA010000619         1673 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_93388_length_1673_cov_3.860939, whole genome shotgun sequence.
ACCESSION   DAOOMA010000619 DAOOMA010000000
VERSION     DAOOMA010000619.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 1673)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1673)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1673
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_93388_length_1673_cov_3.860939"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..731
                     /locus_tag="PLP95_13185"
     CDS             <1..731
                     /locus_tag="PLP95_13185"
                     /inference="COORDINATES: protein motif:HMM:NF012596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HPB46804.1"
                     /translation="INRPERRNALSWDLVARMRSAVQAARDDSGVRVIVLTGAGDKAF
                     CAGADLDTMAAGAGAFALHEQRGQLAGLFLDLYEVGKPTIAKVRGFCLAGGFGLATAC
                     DLVVAAEDATFATPEINVGLWPFMITVPLTRSIPPKKALELMMTGRRISAAEADSLGL
                     LTRVVEAAELDDEVRRLADELASKSSAVMKLGRDSFYSVYGRNAADALAQLHPLLSLN
                     NTLADAAEGIAAFRDKREPEWTDG"
     gene            824..1567
                     /locus_tag="PLP95_13190"
     CDS             824..1567
                     /locus_tag="PLP95_13190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HPB46805.1"
                     /translation="MSESAPVISSNVIDRVATITIDRQDRRNALNFAALEALDSAVAD
                     ALGANVAAIVFTGAGGHFCAGADLKELEDQAFDTRLRTMLSNVADASVVTIAAIGGAC
                     MGLGMQLALACDIRIATEDAYFGIPAAKLGIVVNHWTLNRLTWAVGPGAARHMLLSAE
                     VYRFDDAMRCGLVQSSGDLAAALELAARVTTLAPLAIAGTKLGLDLVEQDIASPAFDA
                     AFDRAWASEDLEEGRRAFAERRPPVFTGR"
BASE COUNT          260 a          555 c          571 g          287 t
ORIGIN      
        1 cgatcaaccg gcccgagcgg cgcaacgcgc tgtcgtggga tctcgtcgca cgaatgcgct
       61 cggcggtgca ggcagcccgc gacgattcgg gggtgagggt gatcgtgttg accggcgccg
      121 gcgacaaggc gttctgcgcc ggtgccgacc tcgacacgat ggccgccgga gccggtgcgt
      181 ttgccctgca cgaacagcgc ggacagctcg ctgggctgtt cctcgatctg tacgaggtgg
      241 gcaagccgac gatcgccaag gtccgcggat tctgcctcgc gggtgggttc gggcttgcca
      301 cggcctgcga cctcgtcgtc gccgcggagg acgccacatt cgccaccccg gagatcaacg
      361 tcgggttgtg gccgttcatg atcaccgtcc cattgacgcg gtcgatcccc ccgaagaagg
      421 cgctggagct gatgatgacc ggccgccgga tctccgcggc cgaggcggat tcgttgggac
      481 tgttgacgag ggtggtggag gcggccgaac tcgacgatga ggttcggcgt ctcgccgatg
      541 agttggcgtc gaaatcctcc gcggtcatga agctcggtcg cgactcgttc tattcggtgt
      601 acggacgaaa cgcggccgac gccctcgccc agttgcatcc gttgctgtcg ctcaacaaca
      661 cccttgcgga cgctgccgaa ggcatcgccg cgtttcgcga caagcgcgaa cccgagtgga
      721 ccgacggctg acgggtcggt cacgctgagc acggctcgtg cccggtgtga ccaccgatcc
      781 cagcagtcct cgagtcccgc agtttctccc gaaaggcatc acgatgagcg agtccgcccc
      841 ggttatctcc tccaacgtca tcgatcgtgt ggcgaccatc acgatcgatc gtcaggatcg
      901 gcgaaacgca ctcaatttcg cggcgctcga ggcgctcgat tcggcggtgg ccgacgcgct
      961 cggcgcgaat gtggcggcga tcgtgttcac gggggccggc ggtcatttct gtgcaggcgc
     1021 cgacctcaag gagttggagg atcaggcctt cgacacccgg ctgcgcacca tgctctccaa
     1081 cgttgccgac gcctcggtgg tgacgatcgc cgcgatcggc ggggcctgca tgggtctggg
     1141 catgcagctg gccctcgcct gcgacatccg catcgcgacc gaggacgcct acttcggtat
     1201 ccccgccgcg aagttgggca tcgtggtgaa ccactggacc ctcaaccgac tgacctgggc
     1261 agtcgggccg ggggccgcac gccatatgtt gctgagcgcc gaggtctacc ggttcgacga
     1321 tgccatgcgc tgtggtctgg tgcagtcatc gggggacttg gcggcagcgc tcgaactggc
     1381 ggcccgggtc acgaccctcg caccgttggc gatcgcgggg acgaaactcg ggctcgacct
     1441 ggtggaacaa gacatcgcca gtcccgcatt cgacgcggcc ttcgaccggg cctgggcgag
     1501 cgaggacctc gaagaggggc ggcgggcctt cgccgagcgt cgcccgccgg tgttcaccgg
     1561 gcgttgacca gaccccaccg ctgaccccgc ctgccgcggc ccagcctgcc gcggcccagc
     1621 ctgttctccg ataaccagtg gtcgtgatca cgaccactgg ttatcggaga acg
//
LOCUS       DAOOMA010000620         1663 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_94303_length_1663_cov_4.000622, whole genome shotgun sequence.
ACCESSION   DAOOMA010000620 DAOOMA010000000
VERSION     DAOOMA010000620.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 1663)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1663)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1663
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_94303_length_1663_cov_4.000622"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..388)
                     /locus_tag="PLP95_13195"
     CDS             complement(<1..388)
                     /locus_tag="PLP95_13195"
                     /inference="COORDINATES: protein motif:HMM:NF012993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HPB46806.1"
                     /translation="MKVAAIQHDICWHDPEPTHAIVDPMIDEAVEGGASLVVLSEMWA
                     TGFSMDPFAVCQAMDGPSVAFMIDRAARHGIWVSGSLAIHDDGLAYNRFIAAGPNGEL
                     VTYDKRHPFSYAGEHKAYSAGADLVSF"
     gene            complement(425..916)
                     /locus_tag="PLP95_13200"
     CDS             complement(425..916)
                     /locus_tag="PLP95_13200"
                     /inference="COORDINATES: protein motif:HMM:NF025698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4395 domain-containing protein"
                     /protein_id="HPB46807.1"
                     /translation="MTSRRFSFPETVDDYAARTVATGVALSGVVLLITRWYWIAPVLA
                     FGFVARVIAGPTFSPLAQFATKVAVPALGGAKRPMPGKPKRFAQAIGATFTIAATIAH
                     FAFGASAVALLLMGMLVVAASLEAGLGFCLGCWMFGRLMKLGVIPDDACVECNDLSMR
                     VPY"
     gene            complement(958..1254)
                     /locus_tag="PLP95_13205"
     CDS             complement(958..1254)
                     /locus_tag="PLP95_13205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB46808.1"
                     /translation="MPLRQVVLRIDERDASGLCVVRCPSCSARITQHACDAMMTALVA
                     VGIEISLAVGDVGEPDPGVPLTETEVNEFADRLKIADDVIGLVGRNDPPTTSVH"
     gene            1468..>1663
                     /locus_tag="PLP95_13210"
     CDS             1468..>1663
                     /locus_tag="PLP95_13210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_process: GO:0046855 - inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HPB46809.1"
                     /translation="MAFDLQTDLNFAESVMRQAGEFTLKHFRSSSLTIDDKSDGSPVT
                     EADRGAEELVRRLIEDRFPDD"
BASE COUNT          353 a          558 c          473 g          279 t
ORIGIN      
        1 cgaagctgac gaggtcagca ccagcgctgt acgccttgtg ttcgccggcg taactgaacg
       61 ggtgccgctt gtcgtaggtc accaactccc cgttcggtcc ggctgcgatg aagcggttgt
      121 aggccagtcc gtcatcgtgg attgcgagcg atccactcac ccagatgcca tgtcgtgccg
      181 cgcggtcgat catgaacgcc acgctcggac cgtccatcgc ctgacacacg gcaaaggggt
      241 ccatggagaa cccggtcgcc cacatttcgc tcaacacgac caacgatgcg ccaccttcga
      301 cagcctcgtc gatcatcggg tcgacgatcg catgagtcgg ttcagggtcg tgccagcaga
      361 tgtcgtgttg tatggcggcg accttcacgg ccccaacgct accggcgcgc tgtgctcgac
      421 gcgctcagta cggcactcgc atggacaagt cgttgcactc cacgcaggca tcgtcgggga
      481 tcacgcctaa cttcatgagg cgaccgaaca tccaacaccc caggcaaaac ccgaggcccg
      541 cctcgagcga cgcggccacg accagcatgc ccatcaacag caaggccacc gccgacgctc
      601 caaaggcgaa gtgagcgatc gtcgcggcga tcgtgaacgt tgcgccgatc gcctgggcga
      661 aacgcttcgg ctttccgggc atcggacgct ttgctccccc cagcgcaggc accgcaacct
      721 ttgtggcgaa ttgggcgagt gggctgaacg tcgggccggc gatcactcgg gcgacgaagc
      781 cgaaggccaa taccggtgcg atccaatacc aacgcgtgat caacagcacg acaccactca
      841 acgccacccc ggtggcgacg gtacgagccg cgtagtcatc gacggtctcc gggaacgaga
      901 atcggcgtga ggtcatgaag ccatgatagc gcctaattcc gaccaaacaa gtcgggatta
      961 gtgaaccgat gttgttggtg gatcatttcg tcctacgagg ccgatcacgt catcggcgat
     1021 cttcaaccga tccgcaaact cgttgacctc cgtttcggtc aacggaactc cagggtcggg
     1081 ttcgccgaca tcaccgaccg caagggagat ctcaatccca accgcgacca acgccgtcat
     1141 catcgcgtca caggcatgtt gggtgatccg agccgaacac gacgggcatc gcacgacgca
     1201 gagaccggac gcgtctcgct catcgatacg cagcaccacc tgccgcaagg gcacctcgaa
     1261 gaccccacaa cgccaacagg tggttctgat caggctccaa cttgttgggc tgtcatcaca
     1321 tttcgccacg ccgcctcgct tcatctcccg aacggtacgt ggacctatcg gtgccggctc
     1381 ggccgcgatg agcgtccggg tccaaaacag gcgaaaaggc ccgggtcgaa cttcacccaa
     1441 cgggtcgctc gcgacgaaac tggcgacatg gccttcgacc tccagaccga tttgaacttc
     1501 gccgaatccg taatgcgcca agcgggcgag ttcacgttga aacacttccg ctcatccagc
     1561 ctgacgatcg acgacaaatc cgacggatcg ccggtcaccg aagccgaccg cggcgccgag
     1621 gaactggttc ggcgtctcat cgaagaccgc ttccccgacg act
//
LOCUS       DAOOMA010000621         1661 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_94486_length_1661_cov_3.277709, whole genome shotgun sequence.
ACCESSION   DAOOMA010000621 DAOOMA010000000
VERSION     DAOOMA010000621.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 1661)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1661)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1661
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_94486_length_1661_cov_3.277709"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..316
                     /locus_tag="PLP95_13215"
     CDS             <1..316
                     /locus_tag="PLP95_13215"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015798229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HPB46810.1"
                     /translation="PVVWACDPMHGNTFTSNGGRKTRHFDAILAEIRGFFEVHRAVGT
                     WAGGVHVELTSDDVTECMGGAEAIGDDDLHLRYETICDPRLNGRQSLDLAFEVSELLR
                     RG"
     gene            390..758
                     /locus_tag="PLP95_13220"
     CDS             390..758
                     /locus_tag="PLP95_13220"
                     /inference="COORDINATES: protein motif:HMM:NF016394.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CD225/dispanin family protein"
                     /protein_id="HPB46811.1"
                     /translation="MADQFPPPPGDQGWVPPPPNYGGYPPPGPPFATQPSGMKPDAYL
                     VQSILVALFCCQPLGIVAIVFSVMSGSAWNRGDVAGANDHAANARKFVKWSLISGLVV
                     VVLYLLIAVIAVLGSSSTSG"
     gene            complement(742..1608)
                     /locus_tag="PLP95_13225"
     CDS             complement(742..1608)
                     /locus_tag="PLP95_13225"
                     /inference="COORDINATES: protein motif:HMM:NF012797.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HPB46812.1"
                     /translation="MTAESTPRVRLDAIVQDPSDERVASFMGLRDRDLRLRARATAAS
                     DDPQEAVFVAEGDPVVERAVRAGYRLITLLVDATRSQPLPEPIAEAVVEGGGEVLGAG
                     PQVLERITGLGVHRGCLGVFARRDLPSAEQVVGGARRLLVLERVVNPINLGVIVRSAV
                     GLGLDAMVLDEASVDPLYRRASRVAMGEVFDFPYARVRRFPEGLDMVHAAGFVTCALT
                     PAPDAVPIDSLRFGAEEKVALLLGSEGPGLSDATMSACDHRVVIPLSGRVDSLNVGAA
                     AAIACYAITRKC"
BASE COUNT          270 a          608 c          527 g          256 t
ORIGIN      
        1 cccggtcgtg tgggcgtgcg acccgatgca cggcaacacc ttcacctcca acggcggccg
       61 caagacccga catttcgacg cgatcctcgc cgagatccgg gggttcttcg aggttcaccg
      121 tgcggtgggc acctgggcgg gcggcgtcca cgtggaactc accagcgatg acgtcaccga
      181 atgtatgggc ggggccgagg cgatcggcga cgacgacctc cacctgcgct acgagacgat
      241 ctgcgaccca cgcctgaacg gtcgccaatc cctcgatctg gccttcgagg tttcagaact
      301 gctccgccgc ggttaaccgg tcggcgggct acgctgcgac ctacccgatc cgaccctgac
      361 cgatccgcta ctgagattgc gaggcaccca tggccgacca gttcccacct ccaccgggag
      421 accagggatg ggttcctccc ccgccgaact acggcgggta tccgcctccg ggcccgccgt
      481 tcgcgacgca gccgagtggg atgaagcccg atgcctacct cgttcagtcg atcctcgtgg
      541 cgctgttttg ttgccaaccg ctcggcatcg ttgcgatcgt gttctcggtc atgtccggca
      601 gcgcgtggaa ccgtggggac gtggccgggg cgaacgatca cgcagcgaac gcccgcaagt
      661 tcgtcaagtg gtcgctgatc tccgggttgg tcgtcgtcgt cctctacctc ctcatcgctg
      721 tcatcgccgt gctggggtca tctagcactt ccgggtgatg gcgtaacacg cgatggccgc
      781 ggcggctccg acgttgagtg agtcgacgcg ccccgacagc ggaatgacga cgcggtggtc
      841 gcacgcagac atcgtcgcgt cgctcaaccc cgggccttcg ctccccaaca acagcgccac
      901 cttctcctcg gcgccgaagc gcagcgaatc gatgggcacg gcgtcgggcg ccggggtgag
      961 cgcgcacgtc acgaaacccg cggcgtgcac catgtcgagg ccctcgggga atctgcggac
     1021 ccgcgcgtag gggaagtcga aaacctcgcc catcgccacc ctcgaggcgc gccggtacag
     1081 cgggtcgacg ctggcctcat cgagcaccat ggcgtcgagg ccgaggccga ccgcgctgcg
     1141 cacgatcacc ccgaggttga tggggttgac gacccgctcc aacaccaaca gccgtcgcgc
     1201 cccgcccacg acctgttccg cgctcggcag gtcgcgccgc gcgaacacgc cgagacaccc
     1261 ccgatgcacc cccagcccgg tgatgcgttc gaggacctga ggtccggcac cgagcacctc
     1321 gccgccgccc tccaccaccg cttctgcgat gggctcggga agcggctgcg aacgcgtggc
     1381 gtcgaccagc agggtgatga gccgatagcc ggcgcgcacg gcccgctcga ccactggatc
     1441 gccctcggcc acaaagaccg cctcctgagg atcatcgctc gcggcggtcg cccgcgcccg
     1501 taggcgcagg tcgcgatctc gcagccccat gaacgacgcc acccgttcgt cgcttggatc
     1561 ctgcacgatc gcatcgaggc gaactcgggg ggtcgactcc gctgtcatct cgctgatcgt
     1621 aggctggcca cgatgccgcg aacgccaccg acgaccacca c
//
LOCUS       DAOOMA010000622         1659 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_94687_length_1659_cov_3.535536, whole genome shotgun sequence.
ACCESSION   DAOOMA010000622 DAOOMA010000000
VERSION     DAOOMA010000622.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 1659)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1659)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1659
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_94687_length_1659_cov_3.535536"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(266..676)
                     /locus_tag="PLP95_13230"
     CDS             complement(266..676)
                     /locus_tag="PLP95_13230"
                     /inference="COORDINATES: protein motif:HMM:NF013098.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HPB46813.1"
                     /translation="MNITIRNSFLPHIDPGASVAFYRDVLGFEVRLDVGYEDMRWITV
                     GPTGQPDVSIVLQPPVVDPGVSEDERRSIAEMMAKGVFAGIVLATPNVDEAFAQIEAH
                     GVEVIQEPTDMPYGARDCAFRDPAGNMVRLQQWD"
     gene            complement(687..1163)
                     /locus_tag="PLP95_13235"
     CDS             complement(687..1163)
                     /locus_tag="PLP95_13235"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HPB46814.1"
                     /translation="MVADHRHLDELVLLRRVRDRIDREHALPLDVESLARDAGMSAGH
                     LSRQFKAAYGEPPYSYLMTRRVERAMMLLRRGDLSVTEVCFAVGFSSLGTFSTRFTEL
                     VSMSPSAYRRLAADANAAATASTPIGATNTNGIPSCVARHATKPIRNREALLRAHP"
     gene            complement(1157..>1659)
                     /locus_tag="PLP95_13240"
     CDS             complement(1157..>1659)
                     /locus_tag="PLP95_13240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HPB46815.1"
                     /translation="LFDAANEHDRLLELARALGVRRIVETHGHWDHIQAVTAMRDAGF
                     SVGVTTEDAAMLPSYDEILDDDTVIQVGELRVRTIHTPGHTPGSMCFVVENKPLLFSG
                     DTLFPGGAGNTSLEGGDFTAIMTSIDERLYRGLDDNTIVMPGHGLDTTIGAEKPHFDE
                     WVDRGW"
BASE COUNT          304 a          497 c          558 g          300 t
ORIGIN      
        1 aactccccga gcgcgtcgat catgtgcaga tccaaacggc gttgcagctc gaacaggacc
       61 gcaacatcgt cgatcccggc acccaggcta ttcagctcgc tgtcgaagcc atcgaacata
      121 tgtatgactc taaacgcgac caccgacaac gaagcccgag acgccgagaa tcctcgagaa
      181 ttctcgagga tctccggaaa tcaaggccag gcacccaagc ctgattggtc aacagggccg
      241 atgtccgctg gcggcggtca gccgatcagt cccactgctg gaggcgcacc atgttgccgg
      301 cggggtccct gaacgcgcag tcccgcgccc cgtacggcat gtcggtcggc tcctggatga
      361 cctcgacacc gtgggcttcg atctgggcga acgcctcgtc gacgttcggg gtggcgagga
      421 cgattccggc aaagacaccc tttgccatca tttcggcgat ggatcgccgt tcgtcctcgg
      481 atacgccggg gtcgacgacc ggcggttgca gcacgatcga gacgtccggt tggcccgtgg
      541 ggccaacggt gatccaacgc atgtcctcgt agccgacgtc gagtcggacc tcaaagccga
      601 ggacgtcgcg gtagaacgcg acagaggcac ctgggtcgat gtggggaagg aatgagttgc
      661 ggatggtgat gttcatggtg tcgaacctac gggtgtgctc gcagcagggc ttctcgattc
      721 ctgatcggtt tggtggcgtg tcgggccacg cacgacggga tgccgttggt gttggtcgca
      781 ccgattggcg ttgaagccgt ggcggcggcg ttggcgtcag cagctagccg ccgatacgcg
      841 ctcggcgaca tggagacgag ttcggtgaac cgagtgctga acgtgccgag ggaactgaat
      901 ccgacggcga aacacacctc ggtcacgctg aggtctccgc ggcgcaacag catcatcgcc
      961 cgctcgacgc gacgggtcat caggtacgag tacggcggct cgccgtaggc agctttgaac
     1021 tgtcgactga ggtgtccggc tgacatgccc gcatcgcgag cgagcgattc gacatcgagg
     1081 ggcagcgcgt gctcgcggtc gatccggtca cgaacccgac gtaacagaac cagttcgtcc
     1141 aggtggcgat ggtcggctac caaccccggt cgacccattc gtcgaaatga ggtttctcgg
     1201 cgccgatggt cgtgtcgagg ccgtggccgg gcatgacgat cgtgttgtcg tcgaggcccc
     1261 ggtagagccg ttcgtcgatg gaggtcatga tcgcggtgaa gtcgccaccc tccaatgacg
     1321 tgttgcctgc accaccgggg aacagggtgt cgccgctgaa gagtaggggc ttgttctcga
     1381 cgacgaaaca catcgacccg ggggtgtggc ctggggtgtg gatggtgcgc acgcgcaatt
     1441 cgccgacctg gatgaccgtg tcgtcgtcga ggatctcgtc gtagctcggc agcatcgcag
     1501 cgtcctcggt cgtcaccccg acggagaatc cggcatcgcg catcgcggtc acggcctgga
     1561 tgtgatccca gtgcccgtgg gtctccacga tgcggcgaac ccctagtgcc cgtgcgagtt
     1621 cgaggaggcg atcgtgctcg ttggcggcat cgaagagca
//
LOCUS       DAOOMA010000623         1659 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_94699_length_1659_cov_2.887157, whole genome shotgun sequence.
ACCESSION   DAOOMA010000623 DAOOMA010000000
VERSION     DAOOMA010000623.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 1659)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1659)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1659
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_94699_length_1659_cov_2.887157"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..308)
                     /locus_tag="PLP95_13245"
     CDS             complement(<1..308)
                     /locus_tag="PLP95_13245"
                     /inference="COORDINATES: protein motif:HMM:NF016724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HPB46816.1"
                     /translation="MATSIRLRPWQKAALEKLLAKDADNFLAVATPGAGKTTFALTVA
                     AQYLSSGLCQGLFVVAPTQHLKVQWAEAAHRIGLHLDDDWAASDGELPSDMHGIVTTY
                     "
     gene            488..901
                     /locus_tag="PLP95_13250"
     CDS             488..901
                     /locus_tag="PLP95_13250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020378395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HPB46817.1"
                     /translation="MQFPDELPDHPARNASVASMTAIENGDRATWLSLFAPDAVVADP
                     IGPSPLDPDGNGHRGLEAIAAFYDTVIGPNQVRFTIKESWEAGNEVANVGRITTTMAD
                     GLVVHTDGVFTYRIDDAGRVEALRAYWSIDRLTYE"
     gene            915..>1659
                     /gene="purU"
                     /locus_tag="PLP95_13255"
     CDS             915..>1659
                     /gene="purU"
                     /locus_tag="PLP95_13255"
                     /EC_number="3.5.1.10"
                     /inference="COORDINATES: protein motif:HMM:NF004684.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008864 - formyltetrahydrofolate
                     deformylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyltetrahydrofolate deformylase"
                     /protein_id="HPB46818.1"
                     /translation="MTILLLSCPDGPGLVAAISGFIQDHGGNIVHADQHADERHDLFL
                     QRIEFTLEPGADHDAFRTEFSLLAQHLGMSWTLAEESDRQRVALLVSKEGHCLLDLLS
                     RHHLGLLNIDIPVVLSNHPDFREITEKFGVPFLHLPVDLDHKSAQQRAVLQALEEHDI
                     DLVVLARYMQILSPEIIDRYPRRIINIHHSFLPAFIGAKPYHQAYDRGVKIIGATAHY
                     ATAELDQGPIIRQDVTPVSHRDDPERLIRM"
BASE COUNT          319 a          584 c          481 g          275 t
ORIGIN      
        1 tgatacgtgg tcacgattcc gtgcatgtcc gacggcaact cgccgtcgga ggccgcccag
       61 tcgtcgtcga ggtgtaagcc gatacgatgc gccgcctcgg cccactgaac cttgaggtgc
      121 tgggtcgggg cgaccacgaa caacccctgg caaagccccg aggacagata ctgcgcggcg
      181 acggtgagcg cgaaggtcgt cttgccggca ccaggggtgg cgacggcgag gaagttgtcc
      241 gcgtccttcg cgagcaactt ttcgagcgca gctttttgcc agggccgaag cctgatcgat
      301 gtcgccatga gggtgggtga gcgtccgtga acgggagtcg gggcaagggg acccaatagt
      361 ctgccagcct ccaccgacat gaccggaacc gttccggtca cacctcggat gcgacccgct
      421 gactcggaag ttcggtaccg tcaacagact gaccctgttc cacccattct caccgaagga
      481 gcccccgatg cagttccccg acgaacttcc cgaccacccg gcgcgcaacg cgtcggtcgc
      541 ctcgatgacc gccatcgaga acggggaccg agcgacctgg ctgtcgctgt tcgctcccga
      601 cgccgtggtc gctgacccga tcggcccctc cccgctcgac cccgatggca acggccaccg
      661 cggtttggag gcgatcgccg ccttctacga cacggtgatc ggcccgaacc aggtgcgctt
      721 caccatcaag gaatcctggg aggccggcaa cgaggtggcc aacgtaggtc gaattacgac
      781 gacgatggcc gatggactag tggtgcacac cgacggggtg ttcacctacc ggatcgacga
      841 cgccggacgt gtcgaggcac tccgcgccta ctggtcgatc gaccgactca cctacgagta
      901 gtccgacagc caagatgacg atccttctcc tctcctgtcc cgacggcccc gggctggtcg
      961 ctgcgatcag tgggttcatc caggaccacg gcggcaacat cgtccacgcc gaccaacacg
     1021 cggacgaacg ccacgacctg tttctgcagc gcatcgagtt cacgctcgaa cccggcgccg
     1081 atcacgacgc gttccgcacg gagttctcgc tgcttgcgca acacctcggc atgtcgtgga
     1141 ccctcgccga agagtccgac cgccaacgag tggcgctctt ggtgtcgaag gaaggtcact
     1201 gcctgctcga cctgttgtcg cgacatcatc tcggcctcct caacatcgac attccggtgg
     1261 tgctctccaa tcaccccgat ttccgggaaa tcaccgagaa gttcggtgtc ccgttcctac
     1321 acctcccggt cgacctcgac cacaagtcgg cccagcagcg cgcagtgctc caagcgctgg
     1381 aggaacatga catcgacctc gtggtgctcg cccgctacat gcagatcctg tcccccgaga
     1441 tcatcgaccg atatccgcgc cgcatcatca acatccacca ctcgttcctg cccgcattca
     1501 tcggggcaaa gccctatcac caggcgtacg accgcggagt gaagatcatc ggagcgaccg
     1561 cccactacgc cacggcggaa ctcgaccaag gcccaatcat ccgccaagac gtcaccccgg
     1621 tgagccaccg cgacgacccg gagcgactca tccgcatgg
//
LOCUS       DAOOMA010000624         1654 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_95150_length_1654_cov_5.026266, whole genome shotgun sequence.
ACCESSION   DAOOMA010000624 DAOOMA010000000
VERSION     DAOOMA010000624.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 1654)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1654)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1654
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_95150_length_1654_cov_5.026266"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..812)
                     /locus_tag="PLP95_13260"
     CDS             complement(<1..812)
                     /locus_tag="PLP95_13260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB46819.1"
                     /translation="MAGLTSSRRRDRRVVYSPVDLLRLVASLIALALVVLFVNFGSSA
                     LSGAESDLIHQAERLPDGFVRLIVRVVQVIALFAPIVGGCVILARRQFRRFAGTLGAA
                     TAGTIIGRWIMTDLLDLSVARFEITGALAEASFPTAAFLAGFSSIVAADSPWMSHRWR
                     RAARVWLFVLLALRVLSGQTGLRELIVAVAVGWVVGSAVGAIVGAPDRRPSELSIASS
                     LARLGVSVAWVKHIKSVSGRHEYQVMTNGAISLRVLVAARDGWQTMLPGRLYR"
     gene            897..>1654
                     /locus_tag="PLP95_13265"
     CDS             897..>1654
                     /locus_tag="PLP95_13265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020379203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HPB46820.1"
                     /translation="MHIALTDEQAALRDELAAYYKQLLTDEIRDALMVEHGCGPVTRQ
                     MVKQMAEDGWLGIGWPVEYGGQGKSQMEQFIFYDESMRSGAPVPMLTINTVGPTIMNY
                     GTQEQKDFFLPKILAGDLHFSIGYSEPEAGTDLASLRTRAELDGDEWVINGQKMWTSL
                     ASDADYCWLAVRTAPDAPKHKGISMIIVDLKNTPGITIEPLNLLSSHDINAVYYDNVR
                     VPKENLVGGLNNGWSLITNQLNHERVTLCSSGLL"
BASE COUNT          361 a          558 c          501 g          234 t
ORIGIN      
        1 cgatagaggc ggcccggcag catggtctgc caaccgtcgc gtgccgcgac gagcacccga
       61 agcgaaatcg cgccgttggt catcacctgg tactcgtggc gtcccgacac cgacttgatg
      121 tgtttcaccc aggccaccga cacgccgagg cgtgcgagcg aggacgcgat gctcagttcc
      181 gagggtcgtc ggtcgggagc tccgacgatc gcgcccaccg ccgagccgac cacccaaccg
      241 accgccaccg cgacgatgag ttcgcgaagc ccggtttggc ccgacagcac ccgcagcgcc
      301 agcaacacga acaaccacac gcgcgccgcc cggcgccatc gatgcgacat ccacggggag
      361 tcggcggcga cgatggagga gaacccggca agaaaggccg cggtcggaaa gctcgcctcc
      421 gccagcgcgc cggtgatctc gaaccgggcc accgacaggt ccaacagatc ggtcatgatc
      481 caccggccga tgatggtgcc ggcggtcgca gcgccgagcg tgcccgcgaa gcggcggaac
      541 tgtcgccgcg ccagaatcac gcaaccaccg acgatcgggg caaacagggc gatcacctgg
      601 acgacgcgca cgatcagccg cacgaacccg tccgggagcc gttcggcctg atggatcagg
      661 tcggattcag ctccggacaa cgctgaggaa ccgaagttca cgaacaacac gacaagcgcg
      721 agggcgatca acgaggccac aagcctcaga agatcgaccg gtgagtacac cactcgacga
      781 tctcgtcgtc gggagctcgt gaggcctgcc atggcttgtt caacctaatc ggcgcagcga
      841 gagccaacgt ggaggcacct ctgacccgca aacgagaacg cgttacagtt cgccccatgc
      901 acatcgcctt gaccgacgaa caggcggctc tgcgagacga actcgccgcc tactacaaac
      961 aactgctgac cgacgagatc agagatgcgc tcatggtcga acacggctgt ggccctgtca
     1021 cccgacagat ggtcaagcag atggccgagg acggctggct cggaatcggc tggcccgtcg
     1081 agtacggagg ccagggcaag tcgcagatgg agcagttcat cttctacgac gagtccatgc
     1141 gttcgggggc gccggtgccg atgctcacga tcaacaccgt ggggccgacg atcatgaact
     1201 acggcacgca ggaacaaaag gacttcttcc tacccaagat cctcgccggc gatctgcact
     1261 tttccatcgg ctactccgaa cccgaggcgg gcaccgacct tgccagcctc cggacccgcg
     1321 ccgaactcga cggtgacgag tgggtcatca acggccagaa gatgtggacc tcgctggcct
     1381 ctgacgccga ttactgctgg ttggcggtgc gcacggcccc cgatgcccct aagcacaagg
     1441 gcatctcgat gatcatcgtc gatctcaaga acaccccggg catcaccatc gaaccgctca
     1501 acctgttgtc gagccacgac atcaacgcgg tgtattacga caacgtccgg gttcccaaag
     1561 agaacctcgt cggcgggctc aacaacggtt ggagcctcat caccaaccag ctcaaccacg
     1621 agcgggtcac cctgtgcagt tcggggctgt tgga
//
LOCUS       DAOOMA010000625         1644 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_96048_length_1644_cov_5.927627, whole genome shotgun sequence.
ACCESSION   DAOOMA010000625 DAOOMA010000000
VERSION     DAOOMA010000625.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 1644)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1644)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1644
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_96048_length_1644_cov_5.927627"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            30..1640
                     /locus_tag="PLP95_13270"
     CDS             30..1640
                     /locus_tag="PLP95_13270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HPB46821.1"
                     /translation="MFDLLTSPEAEGVPGGYVEGPPTAAEVARIAAINAWHDEYDELE
                     AEVRTLAGEINAAHARLATKVATMTSDPRYNLAGDWRSVTHWLIVNCGMTRYMAQLVA
                     QVAESVTDNAMPTVMGLAHDGAISLGMAALAARVACPENEQALAGIATTATTTQAARA
                     FGWYRKLKPGIDDQTPDDQEPTDDETPDDERASGDERPAGSAQGGTAVPTPDTPDATD
                     DDETYWRTWTDEHGRWRVNGRAPIELGALLDEAFEAARRSAQRHGTPQTHNNTDSDSA
                     HNTAAGDQPVSEDPRDSRAPAGERTAIASNYLILYHLARMVIDSAQTDRLTREGNEPF
                     CVNVLVDIDTLVTQRLRPDSVATLLNGTELTPTVVAMLAQAGTLQLLWHQNGVPIKVG
                     TEQRFATPTQRKALRVQYGGCAVPGCGQTRFLHYHHVQPYPHGPTDLDNLVPICSFHH
                     RRIHSGHLNAAIVDGQAQFHDERGRLLGNTMAQHALNATTPPTEHSLRNRIDNIDIDK
                     YTAKPAGAGAPLTSWALSVWVEALLGQP"
BASE COUNT          384 a          653 c          410 g          197 t
ORIGIN      
        1 cgtgttctgg ttcctgatac tctgaacaca tgttcgatct tctcacctca cctgaagccg
       61 agggcgtgcc cggcggatac gtcgagggac cgcccacagc ggccgaagtc gcgcggatcg
      121 ccgcgatcaa cgcgtggcac gacgaatacg acgaactcga agccgaggtg cgaaccctgg
      181 ccggcgaaat caacgcggct cacgcccgcc tggccaccaa ggtcgccacg atgacctcag
      241 atccccgcta caacctcgcc ggcgattggc gttcggtcac ccactggctg atcgtgaact
      301 gcggcatgac ccgctacatg gcccaactgg tcgcccaagt cgccgaatcg gtcaccgaca
      361 acgccatgcc caccgtgatg ggcttggcac acgacggtgc catctcactc ggcatggccg
      421 cgctagccgc acgcgttgcg tgccccgaaa acgaacaagc cctcgccggc atcgccacca
      481 ccgccaccac cacccaggcc gcccgagcgt tcggctggta ccgcaaactc aaaccaggca
      541 tcgacgacca gaccccagac gaccaggagc ccaccgacga cgagacaccc gacgacgaga
      601 gggcatccgg cgacgagcgt ccggccggct cggctcaagg cggcacggcg gttcccaccc
      661 ccgacacccc cgacgcgacc gacgacgacg aaacctactg gcggacctgg accgacgaac
      721 acggacgctg gagagtcaac ggtcgagccc cgatcgaact cggagcccta ctcgacgaag
      781 ccttcgaagc cgcccgccgc agcgcacaac gccacggaac accccaaacc cacaacaaca
      841 ccgacagcga cagtgctcac aacaccgccg ccggggacca acctgtcagc gaggacccac
      901 gcgacagccg ggcacccgcg ggcgaacgca ccgcgatcgc gtcgaactac ctgatcctgt
      961 accacctcgc ccgaatggtc atcgattccg cacaaaccga ccgattgacc cgcgaaggaa
     1021 acgaaccgtt ctgcgtgaac gtcctcgtcg acatcgacac cctcgtcacc caacgcctcc
     1081 gacccgactc ggtcgcaacc ctgctcaacg gcaccgaact caccccaaca gtggtcgcca
     1141 tgttggccca agccggaacc ctgcaactgc tctggcacca aaacggtgtc cccatcaagg
     1201 tcggcaccga acaacgcttc gcgaccccaa cccaacgaaa agcgctacgc gtccaatacg
     1261 gcggatgcgc agtcccagga tgtggccaaa cacgattcct gcactaccac cacgtccagc
     1321 cctaccccca cggcccaacc gatctcgaca acctggtacc gatctgctcg ttccaccacc
     1381 gccgcatcca cagcggccac ctcaacgcgg ccatcgtcga cggacaagca caattccacg
     1441 acgaacgcgg acgcctcctc ggcaacacca tggcccaaca cgcactcaac gccaccacac
     1501 caccaaccga acacagcttg cggaaccgca tcgacaacat cgacatcgac aaatacaccg
     1561 ccaaacccgc cggcgccggc gcaccactca cctcatgggc actctcggtc tgggtcgaag
     1621 cccttctcgg ccaaccctaa aaac
//
LOCUS       DAOOMA010000626         1635 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_96822_length_1635_cov_4.501266, whole genome shotgun sequence.
ACCESSION   DAOOMA010000626 DAOOMA010000000
VERSION     DAOOMA010000626.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 1635)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1635)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1635
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_96822_length_1635_cov_4.501266"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..343
                     /locus_tag="PLP95_13275"
     CDS             <1..343
                     /locus_tag="PLP95_13275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB46822.1"
                     /translation="AYPAELATLFDTVAQSDHGSSLWLAPDRDDKGTNVLVVPTQVAE
                     VFRFGYGPDSATHHHREAESHGLQVRRWTTGRLGIDVDVHGDLDVIDLTRLNAGERPV
                     IEHSDDAHLVR"
     gene            365..1135
                     /locus_tag="PLP95_13280"
     CDS             365..1135
                     /locus_tag="PLP95_13280"
                     /inference="COORDINATES: protein motif:HMM:NF017932.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M90 family metallopeptidase"
                     /protein_id="HPB46823.1"
                     /translation="MAWLSRRRERRAALAEGFRDEWRAAIDDWFEWRSGLDHDQRSRH
                     EALMMRLMFERRWEAGSGFELDDEVRLVIAALAARIAIGLPDDPFRTVTSILVQADDV
                     RMTGEFEQVAGIVSDEESWVSGLSDPTGPVMLSWDAICEDLDYPGDGRNLVYHEFAHQ
                     LDQLDGHSDGVPPIPDAAARTRFIEVCTAAYELAVRGEGATTLDEYAGVNPAEFFAVA
                     TEAFFDAPMTLRGEHRQLYEVLSDYFGQDPAQRPGAAN"
     gene            1153..>1635
                     /locus_tag="PLP95_13285"
     CDS             1153..>1635
                     /locus_tag="PLP95_13285"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HPB46824.1"
                     /translation="MHAASHPTPRPTPLDPPAVALAIAAHPDDVEFCAGATLARWARH
                     GTVVHHLICTDGSKGTWDASADIAALVERRQAEQRAAAAALGATGEVVFLGEVDGELR
                     STSDTVERVCREIRRLRPDVVLAHDPWKRYRIHPDHRAAGFLAIDAVVAARDPHFFAQ
                     Q"
BASE COUNT          264 a          578 c          539 g          254 t
ORIGIN      
        1 cgcctacccc gccgagttgg ccacactgtt cgacaccgtc gcccagagcg atcacggttc
       61 gtcgctgtgg ctggcccccg accgagacga caagggcacc aacgtcctgg tcgtgccgac
      121 gcaggtcgca gaggtgttcc gattcggata cgggcccgac tcggccacac atcaccaccg
      181 ggaagccgag tcgcacgggt tgcaggttcg ccgatggacc accgggcgcc tcggcatcga
      241 cgtcgacgtc catggcgacc tcgacgtcat cgacctcacc cgattgaacg ccggcgagag
      301 gcccgtcatc gaacacagcg acgacgccca cctcgttcgc tgaccagggg cctcgacgaa
      361 cgccgtggcc tggttgtcga ggcgccgcga acgacgagcc gccctggcgg agggcttccg
      421 cgacgagtgg cgcgccgcga tcgatgactg gttcgaatgg cgcagcggat tggaccacga
      481 tcaacgaagc cgccacgagg cgctgatgat gcgcctgatg ttcgagcgac gctgggaggc
      541 cggctcgggg ttcgaactcg acgacgaggt ccgcctggtg atcgccgcgc tggccgcgcg
      601 gattgccatc gggttgcccg atgatccgtt tcgcacggtt acctcgatcc tcgttcaggc
      661 cgacgacgtg cgcatgaccg gcgagttcga acaggtcgcc ggcatcgtga gcgacgagga
      721 atcgtgggtg tccggactgt ccgatcccac cggcccggtc atgttgtcgt gggacgcgat
      781 ctgcgaggac ctcgactatc cgggcgacgg acgcaatctc gtgtatcacg aattcgctca
      841 tcaactcgac caactcgacg gccacagcga cggtgtgccc ccgatacccg atgcggcggc
      901 ccggacgcga ttcatcgagg tgtgcacggc cgcctacgaa ctcgcggttc gcggtgaggg
      961 ggccacgacc ctcgacgaat acgcaggggt gaaccccgcc gagttcttcg ccgtggccac
     1021 cgaggcgttc ttcgatgcgc cgatgacgct gcgcggcgaa catcgccagc tctacgaggt
     1081 gctcagcgac tacttcggac aggacccggc acagcgaccg ggcgccgcga actgagggct
     1141 acgatcgccg cgatgcacgc cgcgtcgcac cccacacctc gcccgactcc gctcgacccg
     1201 cccgccgtcg cgttggccat cgctgcgcac cccgacgacg tcgagttctg cgccggggcg
     1261 acgctcgccc gctgggcacg tcacggcacg gtcgtgcacc acctcatctg caccgacggg
     1321 tcgaagggca cctgggatgc ctcggccgac attgcggccc tcgtcgaacg tcgccaggcg
     1381 gaacaacgcg ccgcggccgc cgcgctcggg gccaccggcg aggtcgtgtt cctcggtgag
     1441 gtcgacggcg agttgcggtc gacctcggac acggtggagc gcgtctgtcg ggagatccga
     1501 cgacttcgcc ccgatgtcgt gttggcccac gatccctgga agcggtaccg gatccacccg
     1561 gatcaccgcg ccgccgggtt cttggccatc gatgccgtcg tggccgcacg cgatccccat
     1621 ttctttgccc agcag
//
LOCUS       DAOOMA010000627         1634 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_96956_length_1634_cov_2.941735, whole genome shotgun sequence.
ACCESSION   DAOOMA010000627 DAOOMA010000000
VERSION     DAOOMA010000627.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 1634)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1634)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1634
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_96956_length_1634_cov_2.941735"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..477
                     /locus_tag="PLP95_13290"
     CDS             <1..477
                     /locus_tag="PLP95_13290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monooxygenase"
                     /protein_id="HPB46825.1"
                     /translation="TSGLVGRLGFDPIGRGGAKLSERWHDGTHSLHGVLTAEFPNFFV
                     VSFVQAGFGLNFVHFLGESTNHIAWMIEHCRDEGIATIEATVEAEDEWMNILWEASGP
                     LARYNRTCTPSYNNSEGERTMLAARSAVFPGPLMEYVEYLEAWRDSGELKGTKTTR"
     gene            complement(602..>1634)
                     /locus_tag="PLP95_13295"
     CDS             complement(602..>1634)
                     /locus_tag="PLP95_13295"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF012946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HPB46826.1"
                     /translation="GEPYTPDWPGLDDYAGALVHPQHWPRDLDLTDKRVVVIGSGATA
                     ATLVPAIAGDAAHVTMLQRSPTYFINRPNSSELADTLRGLDVPEEWTYEIVRRQMMQL
                     TDLLTTTARDHPDYVRDMLLDEVRNQLPEGFDVDTHFTPSYRPWQQRLAAVPDGDLFD
                     AIRGGKVSMVTDTIKRFTPTGIETDGGETLEAEVVVTATGFNLSVLGDIDFTVDGESI
                     DPSTLVSYRGIMFSGLPNLAYVFGYFRASWTLRADIISDFVCRVLTRMDERGDTTVMP
                     RLRDSDADMALLPWVDPENFNPGYLARSLDRMPRRGDREPWTWGSEYPDEKAALEAAD
                     LDDGTLQFS"
BASE COUNT          287 a          517 c          554 g          276 t
ORIGIN      
        1 acgagcggcc tggtcggccg tctcgggttc gacccgatcg ggcgtggtgg cgccaagctc
       61 agcgaacgct ggcacgacgg cacccactcg ttgcacgggg tgctcaccgc cgagttcccg
      121 aacttcttcg tcgtgagctt cgttcaggcc gggttcggat tgaacttcgt ccacttcctg
      181 ggcgagtcga ccaaccacat cgcctggatg atcgaacact gccgcgacga gggcatcgcc
      241 accatcgagg cgaccgtcga ggccgaagac gagtggatga acatcctgtg ggaggcctcc
      301 ggtcccctcg cccgctacaa ccgcacgtgc accccgagct acaacaacag cgagggtgaa
      361 cgcacgatgt tggccgcacg cagcgccgtg tttcccggac cgctcatgga atacgtcgag
      421 tacctcgaag cgtggcgcga ctccggtgag ttgaagggca ccaagaccac ccgctgaggc
      481 tcggcgctgg gcgcgtgctg aggtacagcc gcgaactttc tgctgaaagt gacacgtgat
      541 gtcacgttct gcacaaagtt cgcggcgacg accggccggc ctcccgtcac gagtaccgcc
      601 gtcaggagaa ctgcagggtc ccgtcgtcga gatcggcggc ttcgagcgcc gccttctcat
      661 cggggtattc actgccccac gtccacggct cacgatcacc gcggcgaggc atccggtcga
      721 gtgaccgcgc caggtagccc gggttgaagt tctcggggtc gacccacggc aacagggcca
      781 tgtcggcgtc gctgtcgcga aggcgcggca tcaccgtcgt gtcgccgcgc tcgtccatgc
      841 gggtgaggac gcggcacaca aagtcgctga tgatgtcggc acgaagcgtc caactggccc
      901 ggaaatagcc gaacacatag gccaggttcg gcaggcccga gaacatgatg cctcgatagg
      961 acaccagcgt ggacgggtcg atcgattcgc cgtcgacggt gaagtcgatg tcgccgagta
     1021 ccgagaggtt gaatcccgtg gcggtgacga cgacctcggc ctcaagcgtc tcgccaccat
     1081 cggtctcgat ccccgtcggc gtgaaccgct tgatcgtgtc ggtcaccatc gagaccttgc
     1141 cgccacggat cgcatcgaac aggtcgccgt cgggcaccgc agcgagacgc tgttgccacg
     1201 ggcgatacga cggcgtgaag tgcgtgtcga cgtcgaatcc ctcgggaagc tggttgcgca
     1261 cctcgtcgag cagcatgtct cgcacatagt cggggtgatc gcgtgcggtc gtggtcaaca
     1321 gatcggtgag ctgcatcatc tgacggcgca cgatctcgta ggtccactcc tcgggaacgt
     1381 cgaggccgcg cagcgtgtcg gcgagttcgc tcgagttggg gcggttgatg aaataggtcg
     1441 gcgagcgctg cagcatcgtg acgtgggccg cgtcgccggc gatggcggga acgagcgtgg
     1501 cggcggtggc ccccgagccg atcacgacga cgcgtttgtc ggtgaggtcg agatccctcg
     1561 gccaatgctg tgggtggacg agcgcgccgg cgtaatcgtc caggcccggc cagtcggggg
     1621 tgtagggctc accg
//
LOCUS       DAOOMA010000628         1625 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_97752_length_1625_cov_3.873885, whole genome shotgun sequence.
ACCESSION   DAOOMA010000628 DAOOMA010000000
VERSION     DAOOMA010000628.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 1625)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1625)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1625
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_97752_length_1625_cov_3.873885"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            141..>1625
                     /locus_tag="PLP95_13300"
     CDS             141..>1625
                     /locus_tag="PLP95_13300"
                     /inference="COORDINATES: protein motif:HMM:NF020976.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase D family protein"
                     /protein_id="HPB46827.1"
                     /translation="MASPIDRRRFLTTLGLGSVAVVVSCSDDGRSGDRSETSVDGGTP
                     EPSTVVEVATAGLAENPFRLGVASGDPTPTSVILWTRLIVGDSLTSELVDIPLTWEVA
                     VDEAFGQIIGSGIVEATAQWGYSVHLDATDLAPATEYFYRFRVDDYVSPTGRTRTAPA
                     DDQQLDSLNIVFASCQNYAHGYYTAHDAIAADRADAVFFLGDFIYESAGSPDSFRPFE
                     GDEPTDLWGYRSRYELYLADESLQRSRASAPWIVTWDDHEVENNTAGNTSQDGITGAE
                     FANRRAAAYAAYYEHQPLRVPPPVGPTLELYRSIRFGTLAEFFVLDGRQYRDDQPCGD
                     EILTSRAQCADFEVDRTMLGPDQERWIGEGLAAATTTWKVLAQQTVMSSLVIGDIVLN
                     VDQWDGYPQARDRLLSTIVDHDIDNVVVLTGDIHSAGAGTLHTGPELARIPVATEFVG
                     TSITSNSLIDAFPGGAEMLGQVKFEGIEYLNVVDHGYCRCTITPQ"
BASE COUNT          286 a          596 c          474 g          269 t
ORIGIN      
        1 ttgcccttgc cttcgtcgcc ccactgggta ccgacgacga tcgttgcgga catgcctgcc
       61 tccaatttcg ttgacggcaa agactacagg cgacgctttg ctccgagaaa agtggttccc
      121 accatgagta gcgtcgcacc atggcctctc cgatcgaccg ccggcgtttc ctcacgaccc
      181 tcggactcgg aagcgtcgcc gtcgtggtgt cgtgcagcga cgacggccgg tccggagatc
      241 gttcggaaac cagcgtcgac ggaggcaccc ccgaaccctc cacggtcgtc gaggtcgcga
      301 ccgccgggct cgccgagaat ccgttccggc tcggcgtggc atcgggcgac ccgacgccta
      361 cctcggtgat cctgtggacc cgactcatcg tcggcgactc cctcacctcg gagttggtgg
      421 acattccgct cacgtgggag gtggcggtcg acgaggcctt cgggcagatc atcggatccg
      481 gcatcgtcga ggccaccgcc cagtggggct attcggtcca tctcgatgcc accgacctcg
      541 ccccggccac cgagtacttc taccgcttcc gcgtcgacga ctacgtgtcc ccgaccggcc
      601 gaaccaggac cgcacccgcc gacgaccagc aactcgacag tctcaacatc gtgttcgcct
      661 cgtgccagaa ctacgcccac ggctactaca cggcccatga cgccatcgcc gccgatcggg
      721 ccgacgcggt gttcttcctc ggggacttca tctatgaatc agccggaagc cccgactcgt
      781 ttcgcccctt cgagggcgac gaaccgacgg atctgtgggg ataccgcagc cgctacgagt
      841 tgtacctcgc cgacgagtcg ttgcagcgca gccgcgcctc ggctccgtgg atcgtcacct
      901 gggacgatca cgaggtcgaa aacaacaccg ccggcaacac gagccaggac ggcatcaccg
      961 gcgccgagtt cgccaatcgt cgcgccgccg cctacgccgc gtactacgaa caccaacccc
     1021 ttcgcgtgcc gcctccggtc gggccgaccc tcgagttgta ccgcagcatc cgattcggca
     1081 cgctcgccga attcttcgtc ctcgacggcc gccagtaccg cgacgaccaa ccgtgcggag
     1141 acgagatcct cacgagccgc gcgcagtgtg ccgacttcga ggtggaccga acgatgttgg
     1201 gacccgatca ggagcggtgg atcggcgagg gcctcgccgc ggccaccacc acctggaagg
     1261 tgctcgcaca gcagacggtg atgagttcgc tcgtcatcgg cgacatcgtc ctcaatgtcg
     1321 atcagtggga cggctacccg caggcccgcg accgattgct gtcgacgatc gtcgaccacg
     1381 acatcgacaa cgtcgtcgtg ctcaccggcg acattcattc cgccggtgcc ggcaccctcc
     1441 acaccggccc cgaactcgcc cggatccccg tcgccaccga gttcgtcggc acctcgatca
     1501 cctcgaacag cctcatcgac gcgttccccg gcggagccga gatgctgggg caggtcaaat
     1561 tcgagggcat cgagtacctc aacgtcgtcg accacgggta ctgccgttgc acgatcaccc
     1621 cacag
//
LOCUS       DAOOMA010000629         1623 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_97973_length_1623_cov_2.470663, whole genome shotgun sequence.
ACCESSION   DAOOMA010000629 DAOOMA010000000
VERSION     DAOOMA010000629.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 1623)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1623)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1623
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_97973_length_1623_cov_2.470663"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..613
                     /locus_tag="PLP95_13305"
     CDS             <1..613
                     /locus_tag="PLP95_13305"
                     /inference="COORDINATES: protein motif:HMM:NF024639.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HPB46828.1"
                     /translation="ASRTVSSATAAGTLIEAGERVLLCAGEGAREAVEDAGGELVDDP
                     RAAERVIVGFHRSFDYDAMLGATRAVLGGATLLATNDDPIYPAADGPAPGGGAILASI
                     VAATSQAPTVAGKPHEPMAALIRARFTDSTGSGGSMAPEAPSAGVFVGDSPRTDGAMA
                     ARLGWPFAMVLSGNHTHSDEAWQAPNLADLVEGYLHGSERFGG"
     gene            606..1436
                     /locus_tag="PLP95_13310"
     CDS             606..1436
                     /locus_tag="PLP95_13310"
                     /inference="COORDINATES: protein motif:HMM:TIGR00478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HPB46829.1"
                     /translation="MASRRRLDAELVRRKIVDSRSEAQRLIEKRAVTVNGSIADKAAR
                     QVDPGDAIELVGPKKRYVSRGGDKLAAALDAFGIDPAGMTVLDAGASTGGFTDCCLQR
                     GAARVIAVDVGYNQLHERLRSHERVESHERFHVKDLTPQQFGLVDLLVADLSFISLRS
                     VARPLLSVVAPGADLVVLVKPQFEAGRSEVSKGKGIITDPEIHARTVAEVSEAFEALG
                     ATRLGSIESPIHGAQGNTEFLVWFRRSASELPDADLGGARIVGEARIVGESPPRGTRT
                     "
     gene            1433..>1623
                     /locus_tag="PLP95_13315"
     CDS             1433..>1623
                     /locus_tag="PLP95_13315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB46830.1"
                     /translation="MSAFGFLLHGDRDLARSLGADLTRWLIERGHEVRLLERDAARAG
                     YPTLAFEESEFAAGLDLIVG"
BASE COUNT          249 a          539 c          586 g          249 t
ORIGIN      
        1 cgcgtcgcgg accgtcagct cggcgaccgc ggcgggaacg ctgatcgaag cgggtgaacg
       61 cgtgttgttg tgcgccggcg agggggcgcg cgaggcggtc gaggacgcgg gaggggaact
      121 cgtcgacgat ccccgcgctg ccgagcgggt gatcgtgggg ttccaccgca gcttcgacta
      181 cgacgccatg ttgggtgcca cccgggcggt gctcggcggc gcgacgctgc tggccaccaa
      241 cgacgacccg atctatcccg ccgcggatgg gccggccccc ggcggcgggg cgatcctcgc
      301 ctcgatcgtt gcggcgacga gccaggctcc gacggtcgcg ggcaaacccc atgagccgat
      361 ggcggcgctg atccgggcca ggttcaccga ttccaccggc tctggcggct ccatggcccc
      421 cgaagcaccg tcggctggcg tcttcgtcgg ggattcaccg cgaaccgacg gtgcgatggc
      481 ggcacggctg ggctggccct tcgccatggt gttgtcgggc aatcacacac acagcgacga
      541 ggcctggcag gccccgaacc tcgccgacct cgtcgagggt tacctgcacg ggtcggagcg
      601 cttcggtggc tagccgtcgc cgcctcgacg cggagctggt ccgccgcaag atcgtcgaca
      661 gccgctccga agcacagcgc ctcatcgaaa agcgcgcggt gacggtgaac ggatcgatcg
      721 ccgacaaggc ggcccggcag gtcgacccgg gtgacgcgat cgaactggtc ggcccgaaga
      781 agcgctacgt cagccgcggg ggagacaagc tcgccgcggc actcgatgcg ttcgggatcg
      841 acccagccgg gatgacggtg ctcgacgccg gcgcctcgac cggaggtttc accgattgtt
      901 gcctgcaacg tggtgcagcc agggtgatcg ccgtcgacgt cggctacaac caactccacg
      961 aacggctgcg gtcccacgag cgcgtcgaga gtcatgagcg cttccacgtc aaggatctga
     1021 ccccacagca gttcggactc gtcgacctgt tggtggcgga tctgtcgttc atctcgttgc
     1081 gcagcgtggc tcggccgctg ttgagcgtcg tggcgccggg tgccgacctg gtggtgctcg
     1141 tgaagccgca gttcgaagcc ggccgctccg aggtgtcgaa gggcaagggg atcatcaccg
     1201 atcccgagat ccacgcccgc accgtggccg aggtgagcga ggccttcgag gcgctcggcg
     1261 cgacccgttt gggttcgatc gaatcgccga tacacggcgc ccagggcaac accgaattcc
     1321 tggtgtggtt ccggcgcagc gcttcggaat tgcccgacgc cgacctcggc ggagcccgta
     1381 tcgtcggcga agcccgtatc gtcggagaga gcccgccgcg aggtacccgg acatgagcgc
     1441 attcggattc ttgttgcacg gcgaccgcga cctcgctcga agcctcggtg ccgatctgac
     1501 ccggtggctg atcgaacggg gccacgaggt ccggctgctg gagcgcgacg cggcacgggc
     1561 ggggtacccg acgttggcct tcgaggaatc ggagttcgcc gcgggcctcg acctgatcgt
     1621 ggg
//
LOCUS       DAOOMA010000630         1614 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_98825_length_1614_cov_2.388069, whole genome shotgun sequence.
ACCESSION   DAOOMA010000630 DAOOMA010000000
VERSION     DAOOMA010000630.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 1614)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1614)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1614
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_98825_length_1614_cov_2.388069"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..501)
                     /locus_tag="PLP95_13320"
     CDS             complement(<1..501)
                     /locus_tag="PLP95_13320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015798074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HPB46831.1"
                     /translation="MQGKPTITVDTDINAVWARYADTRDPGLRDALILNYSPLVKFVA
                     GRVAVGLPQNVEQADLVSYGMFGLIDAIDKFEPERGFKFETYAMSRIKGAILDELRSI
                     DWVPRSVRSKVRAVEKAFAKLEAREGRSPSEEEVAAELGWTDEQFKQVLSQISVTGMA
                     ARIAAVC"
     gene            complement(505..1404)
                     /locus_tag="PLP95_13325"
     CDS             complement(505..1404)
                     /locus_tag="PLP95_13325"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HPB46832.1"
                     /translation="MAWDLDAFERSLLSVAASTRGAYVRDMAAFAQWAGEVGSDAPES
                     VDRLLVRRYLAEQAGHGVAASAKSPATVRRRLASLRRYYGWMARQGRIEVDPTSNISA
                     PRGDKKLPRVLSADQLHQMLDESAESPHRSDDPAKQARDDAILELLYGSGLRVSELCG
                     LGPDDIDLAQRAVTVWGKGAKQRRVPISTPAVDALHRHLRARDGHTSTEAVDRGALFV
                     NNRGRRLTPRDAQRILDRRSPVPTHPHTLRHSFATHLLDGGADLRVVQELLGHSDLAT
                     TQIYTHVSKERLRTVYDTTHPRA"
BASE COUNT          265 a          510 c          544 g          295 t
ORIGIN      
        1 gcagacggcg gcgatgcgcg ccgccatgcc ggtcaccgag atctgtgaca acacctgttt
       61 gaactgctcg tcggtccaac cgagctcggc ggcgacctcc tcctcactcg gggaacgccc
      121 ctcgcgggct tcgagtttgg cgaaggcctt ttcgaccgcg cgcaccttcg aacgcacgga
      181 gcgaggcacc cagtcgatcg aacgcagctc gtcgagtatg gcgcccttga tgcggctcat
      241 cgcgtaggtc tcgaacttga agccgcgctc tggttcgaac ttgtcgatcg catcgatgag
      301 gccgaacatc ccgtagctca cgaggtcggc ctgttcgacg ttctggggca accccactgc
      361 aacacggccc gccacgaact tcaccagcgg tgagtagttg aggatcaacg catctcgaag
      421 cccgggatcc cgggtgtctg cgtatcgagc ccacacggca ttgatatcgg tgtcaacggt
      481 gatcgttggc tttccttgca tgcgtcaggc cctcggatga gtcgtgtcgt acaccgttcg
      541 cagccgctcc ttggacacat gggtgtagat ctgggtggtt gccagatccg agtgcccgag
      601 cagctcttga acgactctta gatcggcacc gccgtccaaa agatgagtcg cgaaagaatg
      661 gcgcaacgtg tgggggtgag tgggaaccgg cgagcgtcga tcgagaattc gctgagcgtc
      721 tcgcggggtg agccgtcgac ctcggttgtt gacgaacaac gcgcctcggt cgaccgcttc
      781 ggtcgaggtg tggccatctc gtgcgcggag gtggcggtgt agggcatcga cggccggcgt
      841 ggagatcgga acgcgtcgtt gtttggcgcc ctttccccag acagtgacgg cccgctgagc
      901 caggtcgatg tcgtcggggc ccagcccgca gagctcactg acccgcaacc cggagccgta
      961 cagcagctcg aggatcgcgt cgtcgcgggc ctgcttggcc gggtcgtcgc ttcggtgggg
     1021 cgactcggcg gattcgtcga gcatctggtg cagctggtcg gcgctgagga cccggggcag
     1081 ctttttgtca ccacgcggtg ccgaaatatt cgaggtcggg tcgacctcga tgcgaccctg
     1141 tcgggccatc cagccgtagt agcgacgcaa cgatgcgagg cggcgacgaa ccgtcgccgg
     1201 tgatttcgcg gaggcggcga cgccgtgacc ggcctgctcg gcaagatacc gccgcaccaa
     1261 caggcgatcc accgactccg gagcgtcgct gccgacttcg cccgcccact gcgcaaaggc
     1321 cgccatgtcg cggacgtatg cgccgcgggt cgacgctgcc accgacaaca acgatcgctc
     1381 aaatgcgtcg aggtcccagg ccatgcagac acggtacacc cgacatcgct ctgcgtgcac
     1441 gaccgttcac tcttcgcgcg aaccgcggtg aaacccggcg gttaccgggg tgcgccgtca
     1501 aggccgcggg cgtggcgctt gcgatccggg ctgtgtttgc ggccgcggct gtggctgggg
     1561 ctttggctgc gatggtggct gcagctgtgg ctgtggctgc gccaaacctt ggga
//
LOCUS       DAOOMA010000631         1611 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_99080_length_1611_cov_3.015424, whole genome shotgun sequence.
ACCESSION   DAOOMA010000631 DAOOMA010000000
VERSION     DAOOMA010000631.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 1611)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1611)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1611
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_99080_length_1611_cov_3.015424"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..253)
                     /locus_tag="PLP95_13330"
     CDS             complement(<1..253)
                     /locus_tag="PLP95_13330"
                     /inference="COORDINATES: protein motif:HMM:NF015279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HPB46833.1"
                     /translation="MNKADRAGATETVSDVEQMLNLRPEDGWRPPVVSTVSTSAAGVG
                     ELWDAIWAHRRHLERAAEGERRRRGRTATELRGIVAAQLA"
     gene            complement(321..755)
                     /locus_tag="PLP95_13335"
                     /pseudo
     CDS             complement(321..755)
                     /locus_tag="PLP95_13335"
                     /inference="COORDINATES: protein motif:HMM:NF015279.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
     gene            complement(869..1132)
                     /locus_tag="PLP95_13340"
     CDS             complement(869..1132)
                     /locus_tag="PLP95_13340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifU family protein"
                     /protein_id="HPB46834.1"
                     /translation="MRDKVLEVIEIVRPAIQADGGDVNLIEVDEGAGVVTVELTGACV
                     SCPASTVTLKAGIERIMKDRVPGVTEVRQLGEETVETGTIVSL"
     gene            complement(1212..>1611)
                     /locus_tag="PLP95_13345"
     CDS             complement(1212..>1611)
                     /locus_tag="PLP95_13345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HPB46835.1"
                     /translation="SGRPKHADVATLPYPGIATDVLPMLVALMSIADGTSFATENLYQ
                     GRFRYIGELQRMGADIRVEGHHMAVRGVERLSGAPVRAFDIRAGAALVVAAFGAEGET
                     VIHDAHHIDRGYVDMAAKLRALGASVVATE"
BASE COUNT          272 a          595 c          490 g          254 t
ORIGIN      
        1 gtgcgagctg agccgcgacg attccgcgca gttcggtcgc ggttctcccc cgacggcgac
       61 gctcgccctc agcggcgcgc tccaggtggc ggcgatgcgc ccagatggca tcccacagct
      121 cccccacccc cgccgccgac gtggaaacgg tggacaccac tggcgggcgc cagccgtcct
      181 cgggccgcag attcagcatc tgttcgacgt cggaaaccgt ctcggtcgct ccggcccgat
      241 cggccttgtt caccacgaag acatccgatc tccatgagcc ccgccttgtt ggcctggatc
      301 gcgtcgcccc aacccggatt gaggaccacc acgcacgtgt ccgccgacgc gacgatttcc
      361 acctcgacct gtcccacgcc cacggtttcg accaccacga ccgggaatcc gaccgcgtcg
      421 agcacccgta tcgccgaagg ggtcgccagc gccaacccac cgaggtggcc tcgggtggcc
      481 atcgaacgga tgtagatccc ctcgtcgagg gtctgatcgc ccatgcggat gcgatcgccg
      541 aggatcgcgc caccgctgaa cggggacgac gggtcgatcg ccaacaccgc aaggcgttcg
      601 aggtcggact cggtgcgagc gtgggtgcac aacgcgttcg tcagcgtcga ctttcccgaa
      661 cccggcgctc cggtgattcc caccgtgtag gcggtgccac cgagtgggtg agcgagccgg
      721 ccgaccgtgc gggcatcggg tccgccgcgc tccaccaacg acagcaggcg agcgatcgcc
      781 gcacggcgcc caccccgagc ctcgtcgagg agcgcggcga tacgttgctg ctgtgcggcc
      841 gatgccgcgt cgtcgcccac ggaaccgatt acaacgacac gatcgtgccg gtctcgaccg
      901 tttcctcgcc gagttgacgc acctcggtca caccggggac acgatctttc atgattcgct
      961 cgattccggc cttgagggtc accgtggatg ccggacaact cacacacgca cccgtcagct
     1021 cgaccgtcac gacgcccgcg ccctcgtcga cctcgatgag gttgacgtcg cccccatcgg
     1081 cctggatggc gggccggacg atctcgatga cctcaagcac cttgtctcgc acgtcaaact
     1141 cctgactcgg acctcggcgc tccagcgtag gccccgacca ctcatggttc ccaacgccgg
     1201 gccgacagcg gctattccgt cgccaccacc gaggctccga gtgcacgcag tttggccgcc
     1261 atgtcgacat agccacggtc gatgtgatgg gcgtcgtgga tcacggtttc accctccgcc
     1321 ccaaacgccg cgaccaccaa cgccgctccg gcgcggatgt cgaaggcacg caccggcgca
     1381 cccgaaagtc gctcgacccc acgaaccgcc atgtgatggc cctcgacacg gatgtcggca
     1441 cccatgcgtt gcagctcgcc gatatagcgg aatcggccct gatagaggtt ctcggtggca
     1501 aagctcgtgc catccgcgat cgacatgagc gcgaccagca tcggcaacac gtcggtggcg
     1561 attcccggat agggcagcgt cgcgacatcc gcgtgtttgg gacgtccgct c
//
LOCUS       DAOOMA010000632         1600 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_100119_length_1600_cov_2.911327, whole genome shotgun
            sequence.
ACCESSION   DAOOMA010000632 DAOOMA010000000
VERSION     DAOOMA010000632.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 1600)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1600)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1600
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_100119_length_1600_cov_2.911327"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..327)
                     /locus_tag="PLP95_13350"
     CDS             complement(<1..327)
                     /locus_tag="PLP95_13350"
                     /inference="COORDINATES: protein motif:HMM:NF024822.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4112 domain-containing protein"
                     /protein_id="HPB46836.1"
                     /translation="MTSGHDDSPLVLLRSTRALPIAVPEDAPAEGLVPWYVRRLAWML
                     DDSIPVGTSRRVGIDGFLSFIPGFGDAAGFGFATVVVLAGVRAGCSWVTVLRMTVIAL
                     IESTLGL"
     gene            complement(349..852)
                     /locus_tag="PLP95_13355"
     CDS             complement(349..852)
                     /locus_tag="PLP95_13355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB46837.1"
                     /translation="MNLSRNEPPGRPVGAAAVIATIAGTTMPAGVAGVTIARATYRPS
                     LDSADYIARLPDIDGPGPWLAVAAISLGGIAIAVAALRVVLQLRSILPAWVIAVASAF
                     LGVGFGGGVALITAPATGANIGGGAAMVLGLPALVLASGMLVAMAITSPTTAADTATA
                     RPSSPTT"
     gene            912..>1600
                     /locus_tag="PLP95_13360"
     CDS             912..>1600
                     /locus_tag="PLP95_13360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020378337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HPB46838.1"
                     /translation="MAAPTDLPDDPIERLEALTELLRRYNAEYYELDAPTIPDADYDD
                     LKRQLEHLEAEHPQFAAADSPTTTVGGAPSQIFAEVVHRVPMMSLDNAMDQGELRAWG
                     ERTMKRLESEGFDAADIDYVCELKIDGLAMSLRFEAGDYVQAATRGNGRVGEDVTPNV
                     AGIGPIPRRLADGAGGTGSTGSTGGTGGAGTPEVLEVRGEVYLPVAAFDALNAAQAAA
                     GARLYANPRNTA"
BASE COUNT          277 a          505 c          591 g          227 t
ORIGIN      
        1 gagcccgagg gtcgattcga tgagcgcgat gacggtcatg cgcagcaccg tgacccaact
       61 gcatccggcg cgaaccccgg cgagcacgac gaccgtggcg aatccgaatc cggcggcgtc
      121 gccgaacccg ggaatgaagc tgaggaaccc gtcgatgccg acgcgtcgac tcgtccccac
      181 cgggatcgag tcgtcgagca tccacgccaa gcggcgcacg taccagggca ccaacccctc
      241 ggccggggcg tcctcgggga cggcgatcgg caacgctctg gtcgagcgga ggagcacgag
      301 cggtgagtcg tcgtggccgg aagtcacggc gcggacatta cccagtggtc aggtcgtcgg
      361 cgaggacggg cgggccgtgg ctgtgtcggc ggccgtggta gggctagtga tcgccatggc
      421 gacgagcatt ccgctggcga gcaccagcgc cggcagcccg agcaccatcg ccgcaccgcc
      481 accgatgttc gcgcccgtcg ccggtgcggt gatcaacgcg acgccgccgc cgaagccgac
      541 accgaggaat gctgatgcga cggcgatgac ccaggccggc aggatggatc tgagttgcag
      601 gacaacgcga agtgccgcaa cagcgatggc gatgccgccg agggagatgg ctgcgacggc
      661 gagccatgga ccgggtccgt cgatgtcggg caaacgggcg atgtaatcgg ccgaatcgag
      721 cgatgggcgg taggtggcgc gggcgatcgt cacgccggcg acgccggcag gcatcgtcgt
      781 gccagcgatc gtggcgatca ccgccgccgc gccaacaggt cgcccgggtg gttcgttgcg
      841 ggagagattc atcgcgtcgc ctcgccatcg cttcgtcgag agtgtcaggg gcgaacggta
      901 gtgtgcccgt tgtggccgcg ccgaccgatc ttcctgacga tcccatcgaa cgcctcgagg
      961 cgctcaccga gctccttcgt cgctacaacg cggagtatta cgaactcgac gcaccgacga
     1021 tccccgacgc cgactacgac gacctgaaac gtcagctcga gcacctcgaa gccgagcatc
     1081 cacagttcgc ggcggcggat tcgccgacga cgacggtcgg cggtgcgccg tcgcagatct
     1141 tcgccgaggt agtgcaccgc gtcccgatga tgtcgctcga caatgcgatg gatcagggcg
     1201 aattgcgggc ctggggggag cggacaatga agcggctcga atccgagggg ttcgacgccg
     1261 ccgatatcga ctacgtgtgc gaactgaaga tcgatgggct ggcgatgtcg ttgcgtttcg
     1321 aggcgggcga ctacgtgcag gcggcgacgc ggggcaacgg tcgggtgggc gaggacgtga
     1381 ccccgaatgt ggcggggatc ggtcccatcc cgaggcggct tgctgacggt gctgggggca
     1441 ctgggagtac tgggagtact gggggcactg ggggtgctgg caccccggag gtgttggagg
     1501 tgcgcggcga ggtctacctg ccggtcgcgg ccttcgacgc gctcaacgcc gcgcaggcgg
     1561 ccgccggggc gcggctctac gccaatcccc gaaacaccgc
//
LOCUS       DAOOMA010000633         1597 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_100364_length_1597_cov_3.267185, whole genome shotgun
            sequence.
ACCESSION   DAOOMA010000633 DAOOMA010000000
VERSION     DAOOMA010000633.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 1597)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1597)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1597
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_100364_length_1597_cov_3.267185"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..264)
                     /locus_tag="PLP95_13365"
     CDS             complement(<1..264)
                     /locus_tag="PLP95_13365"
                     /inference="COORDINATES: protein motif:HMM:NF012707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HPB46839.1"
                     /translation="MARSARFRSAAAALAAAALLAAATGCSSDDDASGSNSQDTSGAG
                     SSTISMLADLAGKKIGVQSGTTGESYANEHAPSDAEVVAFDDTV"
     gene            complement(397..1470)
                     /gene="ychF"
                     /locus_tag="PLP95_13370"
     CDS             complement(397..1470)
                     /gene="ychF"
                     /locus_tag="PLP95_13370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012225567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HPB46840.1"
                     /translation="MERFGFVGLPNAGKSSLYNALSGGGALAAPYAFATVDPNVGVAK
                     VPDPRVEALGEMSKTKTLVHASVQFVDIAGLVKGAAQGEGLGNRFLAHIREVDAIVFV
                     LRAFVDDDVPGSHDPLEQLDILETELIYADLETLEKQVDKRRKAAKGDKSILDEVAAM
                     QQAIEVLQSGVALYRSELDDDTRALLRPYFLLTNKAAMAVVNIDEGQLADVESIVAPV
                     REAMGGAEVVGACIQLEAEAVLLDAEERAEMLEALGLGDGALARFLRAAYQMLGLRTY
                     LTTGEKETRAWTFRAGAKAPQAAGVIHTDFERGFIRAETISYDELIAAGSWAKAKELG
                     KVRLEGKDYVVADGDVMEFRFNV"
BASE COUNT          279 a          531 c          505 g          282 t
ORIGIN      
        1 gacggtgtcg tcgaacgcca caacctcagc gtcgctcggg gcgtgctcgt tcgcgtagga
       61 ctcaccggtg gtgccggact gcacaccgat cttcttcccc gcgaggtctg ccagcatgct
      121 gatcgtcgag gacccagcac cgctggtgtc ctgcgagttc gagccggagg cgtcatcatc
      181 gctgctgcaa cccgtcgcgg cggcgagcag ggctgcggcg gcgagtgcag cagcggcgga
      241 acggaaacgg gcggaacgtg ccatcagatg atctcctcat tggcggttgc tcggaaacac
      301 attggcccga aggttagagt ctcggccgac ctgcaggacg cctcagcgat tttgaccgct
      361 ccggagcgag ggtcgccgcc cgcaggttca gaccgctcac acgttgaagc ggaactccat
      421 cacgtcgcca tccgccacga cgtagtcctt gccctcaaga cggaccttgc cgagttcctt
      481 cgccttggcc cacgaccccg cggcgatgag ttcgtcatag gaaatcgtct cggccctgat
      541 gaagccgcgt tcgaaatcgg tgtggatgac accggccgcc tgcggcgcct tggccccggc
      601 tcggaaggtc cacgcgcggg tctccttctc gccggttgtg agatacgtgc gcaaccccaa
      661 catctggtag gcggcgcgca ggaagcgggc gagggcaccg tcgccaagac cgagggcctc
      721 gagcatctcg gcccgttcct cggcgtccaa gagcacggcc tcggcctcga gttggatgca
      781 cgcaccgacg acctcggccc cgcccatggc ctcgcgaacg ggggccacga tggattcgac
      841 atcggcgagt tggccctcgt cgatgttgac caccgccatc gccgccttgt tggtgagcag
      901 gaaatacggc cgaagcaatg cacgggtgtc gtcgtcgagt tcactgcggt acagagcgac
      961 gccgctttgc agcacctcga tggcctgctg catcgcggcg acctcgtcga ggatggattt
     1021 gtcgcccttt gccgccttgc gccgcttgtc gacctgcttt tccagcgtct cgagatcggc
     1081 atagatcagc tcggtttcca agatgtcgag ttgttcgagc ggatcgtgac tgccgggaac
     1141 gtcgtcgtcg acaaacgctc gcaggacgaa cacgatggcg tcgacctcac ggatgtgcgc
     1201 caggaaccgg ttgccgagcc cttcgccctg ggcagcgcct ttgacgaggc cggcgatgtc
     1261 gacgaattgc accgatgcgt gcaccagcgt cttggttttc gacatctcgc cgagggcttc
     1321 cacccgaggg tcgggcacct tggcgacgcc gacgttcggg tcgacggtgg cgaatgcgta
     1381 cggagccgca agcgctccgc cacccgatag tgcgttgtac agcgacgact tccccgcgtt
     1441 cggaagccca acaaacccga atcgttccat ggtgtcgaat acgctacccg ggcaccgccg
     1501 cgcgccacga cccgaggaat ccttcgaagc ctctcagcgc ggaccgcacc agaccgatgt
     1561 ttgaaccgac aaactactcg ccgacgccgg aagggaa
//
LOCUS       DAOOMA010000634         1592 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_100809_length_1592_cov_5.127521, whole genome shotgun
            sequence.
ACCESSION   DAOOMA010000634 DAOOMA010000000
VERSION     DAOOMA010000634.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 1592)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1592)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1592
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_100809_length_1592_cov_5.127521"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1282)
                     /locus_tag="PLP95_13375"
     CDS             complement(<1..1282)
                     /locus_tag="PLP95_13375"
                     /inference="COORDINATES: protein motif:HMM:NF012770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HPB46841.1"
                     /translation="MSSRSRGVRRIAALSIAAIVAVSGCTSGKSGSISPQGDSGDTGA
                     STPDETGALDWSRCDGDTIAVESLVLNQAAAKGMKCASMEVPVDHEEPDGATITLALA
                     RVPATKPSERIGSLVFNPGGPGGSGLEFLASSAALISPDIAERFDLVSFDPRGLGASS
                     PLECLTPDQREEWISRSSDEVADTPEMALEFETAVAQGCEADDAALYANIGTDAVIED
                     LDLIREALGEDQLNFMGISYGTRIGALYASRHPDRVRAIILDSPVTPDRSLAALAGGQ
                     AASLAESLAQFYRWCDADPECALQPDAASAARSQAERLEVTPIEVEGENGLEQLDGSM
                     FETALITAMYDPAMSQPLAEAIVALDASGAERERAAAFLLRLAGLQHSRQPDGTYGNG
                     FEVQTLVSCADAEAPLSSQEAEQIAESTPTLLLPD"
     gene            complement(1397..>1592)
                     /locus_tag="PLP95_13380"
     CDS             complement(1397..>1592)
                     /locus_tag="PLP95_13380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB46842.1"
                     /translation="PEVVRLLRAEGVTCPVVAGGIIPTDDRPKLLADGIAAVFSPKDY
                     ELAELMTAIADLALRHRDET"
BASE COUNT          241 a          498 c          579 g          274 t
ORIGIN      
        1 cgtcgggtag cagcagggtg ggggtcgatt ccgcgatctg ctcggcctcc tgggagctca
       61 agggcgcctc ggcatcagcg cagctgacga gcgtttggac ttcgaacccg tttccgtagg
      121 tgccgtcggg ctgccgtgaa tgctgcaggc cggcgaggcg caacagaaag gcggcggcgc
      181 gttcgcgttc ggcgccgctg gcgtcgagcg cgacgatcgc ctcggcgagc ggctgggaca
      241 tcgccgggtc atacattgcg gtgatcaggg cggtttcgaa catcgaaccg tcgagttgtt
      301 ccagcccgtt ctcgccttcg acctcgatcg gggtgacttc gagtcgttcg gcctgtgacc
      361 gggccgcact cgctgcgtcc ggctgaagcg cgcactcggg atcggcgtca caccaccggt
      421 agaactgagc gagactctcg gcgaggctgg ccgcctggcc accagcgagc gctgcgagcg
      481 atcggtcggg ggtcaccggg gagtccaaga tgatggcgcg gacacggtcg gggtgccggc
      541 tggcgtacag cgctccgatg cgtgtcccgt agctgatgcc catgaagttc agctggtcct
      601 cgccgagcgc ctcgcgtatg aggtcgaggt cctcgatgac cgcgtcggtg ccgatgttcg
      661 cgtacagggc ggcgtcgtcg gcctcgcaac cctgtgcaac ggcggtttcg aattccaggg
      721 ccatctccgg agtgtcggcg acctcgtcgg aggagcggga gatccactcc tcgcgttggt
      781 cgggggtgag acactcgagc ggactcgatg ctccgagccc gcgagggtca aaggacacga
      841 ggtcgaaacg ttccgcgatg tcggggctga tgagcgctgc gctcgaggcg aggaactcca
      901 ggccggagcc gccgggtccg ccggggttga acaccagcga cccgattcgt tcgctgggtt
      961 tggttgcggg aacccgcgcg agcgcgagcg tgatcgtcgc cccgtcgggt tcctcatggt
     1021 cgacggggac ctccatcgag gcgcatttca tgcccttggc ggccgcttga ttcaacacga
     1081 gtgactcgac cgcgatggtg tcgccgtcgc agcgcgacca gtcgagggcc ccggtttcgt
     1141 ccggtgtcga agcgcccgta tctccggagt ccccctgtgg cgagatcgag ccggacttgc
     1201 ccgaggtgca accggatacg gcgacgatgg cggcgatgct gagcgcggcg attcggcgca
     1261 cccctcgaga tctcgatgac atggcgtcga ccgtatcggt ccgcgccgac tgaagcgggg
     1321 caccgacctc aatcgtgctg tgcgtcgtca catgagcgcg gcgttggtgg ccgacaccgc
     1381 acgacgtggg cagtacctac gtctcgtcgc gatggcggag cgcgagatcg gcgatcgcgg
     1441 tcatcagctc ggcgagttcg tagtccttcg gggaaaacac tgcagcgatc ccgtcggcga
     1501 gcagcttcgg ccggtcgtcg gtggggatga tgccgccggc caccaccgga caggtgacgc
     1561 cttcggcgcg aaggaggcgc acgacctcgg ga
//
LOCUS       DAOOMA010000635         1586 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_101408_length_1586_cov_3.401698, whole genome shotgun
            sequence.
ACCESSION   DAOOMA010000635 DAOOMA010000000
VERSION     DAOOMA010000635.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 1586)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1586)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1586
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_101408_length_1586_cov_3.401698"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..310
                     /locus_tag="PLP95_13385"
     CDS             <1..310
                     /locus_tag="PLP95_13385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HPB46843.1"
                     /translation="TLAVRHEVLAVEVIDPRELELPDVGVVTLHDTETGRLREVDTSS
                     RRLRADYARVAAEQRRAIAAAITSSGAHHLRLRTDEDWLLALAQFVASNRRHLTMGGR
                     "
     gene            419..1393
                     /locus_tag="PLP95_13390"
     CDS             419..1393
                     /locus_tag="PLP95_13390"
                     /inference="COORDINATES: protein motif:HMM:NF012321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HPB46844.1"
                     /translation="MDWKFLDAQRLWWLVAVAALVVGYIAVQFRRRRSAVRFSNVNLI
                     DKIAPKSPGWKRHLVAALHLCALAVLVTAYARPQNEQRVPRERTTIILAIDTSLSMYA
                     TDVDPSRIESAKSAAVDFVESVPANLQLGVVSFNGSTSLLVPPTTNRRQAVTAIDGME
                     LGEGTAIGDAVNTSLKAIASVPADSDGERPPAVIVLLSDGMTTVGTPTEDAIQPALDA
                     GVPVYTIAFGTADGYVEVDDGTGTGNTEIVPVPVDTDALGNLAEQTHGQFFEAASTKD
                     LTKVYEELGSSIGYDIERVEITAKVLGWGLGLLALSSVLSLWWYQRLP"
BASE COUNT          261 a          544 c          510 g          271 t
ORIGIN      
        1 caccctcgcg gtgcgccacg aggtcctcgc cgtggaggtc atcgaccccc gcgaactcga
       61 gttgccggac gtcggcgtcg tcaccctgca cgacaccgag accgggcgac tgcgcgaggt
      121 cgacacctcg tcgcggcgac tgcgcgccga ctatgcccgc gtcgccgccg aacagcgtcg
      181 cgctatcgct gcggccatca cctcgtcggg ggcccaccat cttcggttgc gaaccgatga
      241 ggactggctg ttggccctcg cccaattcgt tgcgtcgaac cggcgccacc tgacgatggg
      301 cgggcggtag cacccgccat gatcgccgcc gcgctggaac ccccactgga actcgcacgg
      361 gaattcgccc tgggactcgc cccagaacta gccccgggaa ggaactgagg aaacaccgat
      421 ggattggaag tttctcgatg cccagcgcct gtggtggctg gtggccgtcg ctgcgctcgt
      481 cgtcggctac atcgcggtgc agtttcgtcg tcggcgaagc gccgtgcggt tcagcaacgt
      541 caacctgatc gacaagatcg caccgaagag ccccggctgg aaacgccacc tcgtcgcggc
      601 gttgcacctg tgtgcgctcg ccgtgctcgt caccgcctat gcccgaccgc agaacgaaca
      661 gcgggtgccc cgggaacgga ccacgatcat cttggcgatc gacacgtcgc tgtcgatgta
      721 cgccaccgac gtcgacccgt cgcgcatcga atcggccaag tccgccgccg tcgatttcgt
      781 cgagtcggtt cctgccaacc tgcagctcgg ggtggtgagt ttcaacggca gcacctcctt
      841 gttggttccc ccgacgacca accgtcgcca ggcggtgacc gcgatcgacg gtatggaact
      901 cggcgaggga accgcaatcg gcgatgccgt caacacctcg ctgaaggcca tcgcctcggt
      961 gcccgccgac agcgacggcg agcgcccgcc ggcggtcatc gtgttgctct ccgacgggat
     1021 gaccaccgtc ggcacgccca ccgaggatgc gattcagccg gcgctcgacg ccggcgtgcc
     1081 cgtctacacc atcgcctttg gcaccgccga cggatacgtc gaggtcgacg acggaacggg
     1141 aacgggcaac accgagatcg tgccggtccc ggtcgacacc gacgccctcg ggaacctcgc
     1201 cgaacagacc cacggacagt tcttcgaagc cgcctccacc aaggacctca ccaaggtcta
     1261 cgaggagttg ggcagctcga tcggttacga catcgaacgg gtcgaaatca ccgcgaaggt
     1321 cctcgggtgg ggcctcgggt tgctcgcgtt gtcgagcgtg ctgtcgctgt ggtggtacca
     1381 gcgcctgccg tagtcggctg gtcggctggc cggctggtcg gctggtcggc cctgagcatt
     1441 tcgggctcgt ctggttcatc gggcgttcgt ggttcccgaa gatctggccc acccggcttc
     1501 gctgtcggag gtcgcgttta gtatcaaacg catgttcgag ggcttcgaca gcgaactgaa
     1561 tagcctgggt tccgggatcg acgatg
//
LOCUS       DAOOMA010000636         1578 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_102164_length_1578_cov_3.629678, whole genome shotgun
            sequence.
ACCESSION   DAOOMA010000636 DAOOMA010000000
VERSION     DAOOMA010000636.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 1578)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1578)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1578
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_102164_length_1578_cov_3.629678"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1150
                     /locus_tag="PLP95_13395"
     CDS             <1..1150
                     /locus_tag="PLP95_13395"
                     /inference="COORDINATES: protein motif:HMM:TIGR01499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HPB46845.1"
                     /translation="AVPRMLHETPAEALVTASGLRVGTYLSPEGTVQERIRIDGAPID
                     DASLADAVLTVRNVADAIDVTLSAFEAVTLTALVAFADAPVDVAVVEVGLLGRFDATN
                     IIDGDVAVVTSVGGDHTDFRDGWLERVAAEKAGIIKPSSVVVLGDLDDALVPIFAGED
                     HEHLTRFGHDFELVDDRVALGGRQIEVVTSHRSQLGALVPLHGAHQSVNAAVALEATE
                     AVLHVNLTDETVAAAFDSLSLRGRVEVAAVEPLIVLDGGHNPDAAAALGSTLAESFTV
                     AGRRVGVVAMLAGRSPHRYLEALTSHFPLDLVIAASLGGERGLDGSAIVDAAQALGLA
                     VVESPTLEAGLRRAIEGGDESDLIVVCGSFRVLEPAHRTLKALQHEHP"
     gene            1228..>1578
                     /locus_tag="PLP95_13400"
     CDS             1228..>1578
                     /locus_tag="PLP95_13400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB46846.1"
                     /translation="MSRPSGQPTDSNSSRRRSRRPQRHQRGQRGATLVEYTLFVALFV
                     LVAVGAVGGLNSGAKSYFANSSSRIGEPINNRTEVDLTGTTTVGGGWGGGGATTTIAP
                     ASTTTAASTTTAAPT"
BASE COUNT          247 a          522 c          543 g          266 t
ORIGIN      
        1 cgccgttccg cggatgctcc acgaaacgcc ggcggaggcg ctcgtcaccg cgagcgggct
       61 tcgagtgggc acctatctca gtcccgaggg cacggttcag gaacgcatcc gcatcgatgg
      121 agcaccgatc gacgacgcct cactggccga cgcggtgttg acggtgcgca atgtcgcgga
      181 cgccatcgac gtgacactgt cggcgttcga ggcggtgacg ctcacggcac tcgtcgcctt
      241 cgccgatgct ccggtcgacg tcgcggtggt cgaggtcggg ttactcggcc gattcgatgc
      301 gaccaacatc atcgatggtg atgtggccgt ggtgacctcg gtgggcggcg atcacaccga
      361 ctttcgcgac ggctggctcg aacgggttgc ggccgagaag gctggaatca tcaaaccatc
      421 atcggtcgtg gtgctcggcg acctcgatga cgcactggtg cccatcttcg ccggcgagga
      481 ccacgagcac ctgacccggt tcggacacga tttcgaactt gtcgacgacc gggtggctct
      541 cggcggccgt cagatcgagg tcgtgacgtc gcaccgtagc caactcggcg cactcgtccc
      601 gttgcacggg gcacaccaga gtgtgaatgc ggcggtcgcg ctcgaggcga ccgaggcggt
      661 gctgcacgtg aatctgaccg acgagaccgt cgccgcagcg ttcgacagcc tgtcgttgcg
      721 cggccgagtc gaggtcgcag ccgtcgagcc actcatcgtg ctcgacggcg gccacaaccc
      781 cgatgcggct gcagcgttgg gctcgacgct ggcggagtcc ttcacggtcg ccggccgtcg
      841 agtcggggtg gtggccatgt tggcgggccg ttcgccccat cggtatctcg aggcgctgac
      901 ctcgcacttt ccgctcgatc tcgtcatcgc agcctcgctg ggcggcgagc gtggactcga
      961 cggttcggcg atcgtcgacg cggcgcaggc cctcggtctg gcggtggtcg agtcgccgac
     1021 gctggaggcc ggcctgagac gtgcgatcga gggtggcgat gaatcggacc tgatcgtggt
     1081 gtgcgggtcg tttcgggtgc tcgaaccggc gcatcggaca ctcaaggcgc tccaacacga
     1141 acacccctag cggggttccg ctcggaaaat tcgtcaagtc gcggccgcgg gctgtcgata
     1201 cgacgggtgc acgccgagga ggagcccatg tctcgaccga gcggtcaacc gaccgattcc
     1261 aacagctcac gtcgccgctc tcgtcggcca cagcggcacc agcgcggtca gcgcggggca
     1321 accctggtcg aatacacgct gttcgtggcg ctgttcgtgc tcgtcgccgt cggcgcggtc
     1381 ggtgggctca acagtggtgc caagtcgtac ttcgccaact cctcctcgcg aatcggcgag
     1441 ccgatcaaca atcgcaccga ggtcgacctg accggcacga cgacggtcgg tggtggctgg
     1501 ggcggtggcg gcgccacgac cacgatcgcc ccagcatcca cgaccacggc tgcatccacg
     1561 accacggctg cgcccacg
//
LOCUS       DAOOMA010000637         1566 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_103369_length_1566_cov_3.219060, whole genome shotgun
            sequence.
ACCESSION   DAOOMA010000637 DAOOMA010000000
VERSION     DAOOMA010000637.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 1566)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1566)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1566
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_103369_length_1566_cov_3.219060"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(140..1507)
                     /locus_tag="PLP95_13405"
     CDS             complement(140..1507)
                     /locus_tag="PLP95_13405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015799362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HPB46847.1"
                     /translation="MTVIDRSVLARRLASEEQRFIETHPKAAAVAAGGSSLLAGVPMA
                     WMTRWPGAFPIIVDEASGARFVDIDGHSYVDFCLGDTGAMTGHGLAQVADAIERQARR
                     CITTMLPSPDAAWVGDELARRFGLASWQMAMTATDANRFVLRFARHLTGRPKVLVFNW
                     CYHGTVDETFAVLDDQGRVVTRPGTIGSPVPVSDTTKVVEFNDLDALEAALAPGDVAA
                     VLAEPALTNIGIVLPDPGYHEALREICDRTGTLLVIDETHTLCVGPGGATREWDLRPD
                     FFVIGKAIAGGVPAAAYGMSAEIAERLGPVLGDHTIDTAGVGGTLTGNALALAAIRAT
                     LSSTLRGEDFARMIPLAQAWSDGVQSVIDGAGLAWSVNRLGCRSEYWFCPPPRNGRDA
                     AAAVDEDLEAYLHLHALNRGVLLTPFHNMALMSPAHTSSDVEAHTAAFREAVDALVEP
                     RSAEG"
BASE COUNT          254 a          549 c          525 g          238 t
ORIGIN      
        1 ttcccgcgct gtcggcaccg gagaccacgg cgatctggcc caacaactcg ccagcggcgc
       61 gcagcggcgg ccgtgaccgc ggcacctccg cgccggcagc cgggtccgcg ttcgaatccg
      121 tactcgtcat cgacgattcc tacccttcgg ccgaccgagg ttcgaccagt gcatcgacgg
      181 cctcgcgaaa cgccgcggtg tgggcctcga cgtcggacga ggtgtgcgcc gggctcatca
      241 gcgccatgtt gtgaaaaggc gtcagcaaca ccccgcggtt gagcgcgtgc aggtggaggt
      301 aggcctccag atcctcgtcc acggcggcag ccgcatcgcg cccattgcgg ggcggcgggc
      361 agaaccagta ctcgcttcga cacccgaggc ggttgacact ccacgccaac ccggcgccgt
      421 cgatgaccga ctgcacaccg tcgctccagg cctgggccag cggaatcatc cgggcgaaat
      481 cctcaccgcg cagggtactc gacagggtgg cgcggatcgc cgccaaggcg agcgcgttgc
      541 cggtcagggt tccgccgacc cctgcggtgt cgatggtgtg atccccgaga accggaccga
      601 gccgctcggc gatctccgcg ctcatcccgt aggccgccgc tggcacaccg ccggcgatgg
      661 ccttaccgat gacgaagaag tcgggccgca gatcccattc gcgggttgcg ccgcccggtc
      721 cgacacacag cgtgtgggtc tcgtcgatca ccaacagcgt gccggtgcgg tcgcagatct
      781 cgcgtaacgc ctcgtggtat ccggggtcgg gcaggacgat gccgatgttg gtgagcgccg
      841 gttcggccaa cacggcggcc acgtcgccgg gggcgagcgc ggcctcgagc gcatcgagat
      901 cgttgaactc gacgaccttg gtggtgtccg acaccggcac cggtgaaccg atcgttcccg
      961 gccttgtgac gacccggccc tggtcgtcga gcaccgcgaa ggtctcgtcg acggtgccgt
     1021 gatagcacca gttgaacacg agcactttcg ggcggccggt gagatggcgg gcgaatctca
     1081 gcacgaaccg gttggcgtcg gtggcggtca tcgccatctg ccacgaggca agcccgaacc
     1141 ggcgggccaa ctcgtcgccc acccaggcgg cgtcggggct cggcagcatc gtggtgatgc
     1201 agcgacgcgc ctgccgctcg atcgcgtcgg cgacctgggc gagtccgtgg ccggtcatcg
     1261 ccccggtatc accgaggcag aagtccacat acgaatgacc gtcgatgtcg acgaaccgcg
     1321 ctccgctcgc ctcgtcgacg atgatcggaa acgctcccgg ccatctcgtc atccatgcca
     1381 tcggcacgcc ggcgagcaac gatgacccgc ccgccgcaac cgcggcggcc ttcggatggg
     1441 tctcgatgaa gcgctgttcc tcgctggcaa ggcgccgagc gagcaccgat cggtcgatga
     1501 cggtcatcgc gaaagcctac gccttggccg aaccgctcat cggggtgttt gcacctcgtc
     1561 gcgaac
//
LOCUS       DAOOMA010000638         1566 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_103373_length_1566_cov_3.137657, whole genome shotgun
            sequence.
ACCESSION   DAOOMA010000638 DAOOMA010000000
VERSION     DAOOMA010000638.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 1566)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1566)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1566
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_103373_length_1566_cov_3.137657"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..235
                     /locus_tag="PLP95_13410"
     CDS             <1..235
                     /locus_tag="PLP95_13410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB46848.1"
                     /translation="VIGAAPARSSSGAREVEAFAAAEVGAVSVVVVVGAAEVDGAGAA
                     VVSTVGNVDTGSPNSVVVGAKAPARVMKKSVPA"
     assembly_gap    249..258
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<259..622)
                     /locus_tag="PLP95_13415"
     CDS             complement(<259..622)
                     /locus_tag="PLP95_13415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB46849.1"
                     /translation="MGITGRITAVFALVCCGFVAGSAAAEASCVPPSIDVVPAYGAPG
                     DRITVFGEAWATSCDDVVPLPEGQEPARAVENIDITMWNYTGVSRPTSVATASANDQF
                     QFRTSFVIPDDVPAGVTTL"
     gene            838..>1566
                     /locus_tag="PLP95_13420"
     CDS             838..>1566
                     /locus_tag="PLP95_13420"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HPB46850.1"
                     /translation="MYRTRRTAVSAGRGLSAIALALALVASGCGDSEEVADPDATTTT
                     AASGEMPGGDADCSPVEWDDVADGGEFVDYAQLVSAGDNTSFDPAAVQTLDESQITNS
                     LFDGLTDFDYSNGCEPELKGLAAESWEVNDDSTEFVFTIKEGLEFSNGEPVLPHNFKQ
                     AWERLGSKELGAAYSYLISFIKDGSKLNEGELDTLDSIVADDEAMTLTVTLEAPLSDF
                     PAIVSFAPLSPITDVVHGTYRFVRR"
BASE COUNT          285 a          478 c          526 g          267 t
ORIGIN      
        1 cgtcattggc gctgcaccag cccgttcgtc gtcgggggct cgtgaggtcg aggccttcgc
       61 tgcggccgag gtcggggcgg tctcggtcgt cgtcgtggtc ggggctgccg aggtggacgg
      121 tgccggcgct gcggtggtgt cgacggtggg caacgtcgac accggttcgc cgaacagcgt
      181 cgtagtgggc gccaaggcgc cagcgcgggt gatgaagaag tcagtgcctg cgtagtgggt
      241 gtagccctnn nnnnnnnntc aaggttgtca ctccggctgg aacatcgtcg ggaatgacga
      301 acgaggtccg gaactggaat tgatcgttcg ccgaagccgt cgccaccgac gtcggcctcg
      361 aaaccccggt gtagttccac atggtgatgt cgatgttctc gaccgcgcgc gctggctcct
      421 gcccctccgg cagcgggacg acatcgtcgc aactcgtcgc ccaggcctcg ccgaagaccg
      481 tgatgcggtc gccgggcgct ccgtaggccg gaacgacgtc gatcgaaggg ggcacgcacg
      541 acgcttcggc cgccgccgac ccggccacga acccgcaaca cacgagtgcg aaaacggcgg
      601 tgatgcgtcc ggtgatgccc atggcgctca atctaggtcg gcgccccgac tcgaacccgg
      661 aggaacgcgt ctcgaaaccg gaccaacgat cacacgcaaa tcgaccgcat ggcgatagat
      721 tcagaacgac gcatcaattc ggtgcgccgt caggctcgaa cggttgaggc tggggggatg
      781 gcggtgacct tgcaagccga tgatcgcccg agtgccccgg caccaaggag tgtgagcatg
      841 tataggacga gacgaacggc agtttcggcc ggacgcggat tgtcggcgat tgcgctggca
      901 ttggcgctgg ttgcatcagg ctgtggcgat tcggaggagg tggccgaccc cgatgccacc
      961 acgacgaccg cggcctcggg ggagatgccc ggcggcgacg ccgactgcag tccggttgag
     1021 tgggacgatg tcgcggacgg cggcgaattc gtcgactacg cacagctggt gagcgctgga
     1081 gacaacacca gcttcgatcc ggcggcggtt cagaccctcg atgagtctca aatcacgaac
     1141 agtctcttcg acggactgac cgacttcgac tactcgaatg gctgtgagcc cgaactcaag
     1201 ggcctcgcag cagagtcgtg ggaggtgaac gacgactcga ctgaattcgt gttcacgatc
     1261 aaagaaggcc tcgagttctc gaacggcgag ccggtgctgc cccacaattt caaacaggcg
     1321 tgggagcgtc tcggctccaa ggaactcgga gccgcgtact cgtatctgat ctcgttcatc
     1381 aaagatgggt cgaagctgaa cgagggtgaa ctcgacacgc tcgattcgat cgtggccgat
     1441 gacgaggcca tgaccctcac ggtcacgctc gaggcgccgc tgtcggattt cccggcgatc
     1501 gtctcgttcg ctccgctgtc gccgatcacc gacgtcgtcc acggcaccta ccgcttcgtc
     1561 cgccgc
//
LOCUS       DAOOMA010000639         1565 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_103517_length_1565_cov_2.318543, whole genome shotgun
            sequence.
ACCESSION   DAOOMA010000639 DAOOMA010000000
VERSION     DAOOMA010000639.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 1565)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1565)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1565
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_103517_length_1565_cov_2.318543"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(85..1233)
                     /locus_tag="PLP95_13425"
     CDS             complement(85..1233)
                     /locus_tag="PLP95_13425"
                     /inference="COORDINATES: protein motif:HMM:NF012656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HPB46851.1"
                     /translation="MDFELTEAERELAHLCRDFARNEIAPRAPEAWEAAVCPTDLLRE
                     MGELGLLGMLIPERWGGIEMTTVGFVAAMEQIGHADQSVAAAWQAHVTIGSLPLLLFG
                     NDDQRERWLRRLAEGTALGAFGLTEPDAGSDARGIRTRAERVDGGWLINGAKTFISNA
                     GTDMSFGVTLLARTGSDDNAKFASFVVEKDTSGFTMGPKMRGIGWKGLDTRELFFDDV
                     FVPEENLIGDPEMGLSQFLSTLEVGRISIAALSLSLTQAVLDMAVDYAHQRQQFGQPI
                     AKFQAIQFKLADIATELEAARWLTYRAAALRDAGKPFLKEASMAKLKASRLAMSAASE
                     AVQIHGGLGYMLESPVARFYCDAKVLEIGEGTNEIQHMVIGRTLTPGA"
     gene            1296..>1565
                     /locus_tag="PLP95_13430"
     CDS             1296..>1565
                     /locus_tag="PLP95_13430"
                     /inference="COORDINATES: protein motif:HMM:NF024842.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding protein"
                     /protein_id="HPB46852.1"
                     /translation="MQPNDLEPSDRGVGVDVLIVGAGVTGIYQLYRALECGFSAEIVE
                     AGSGVGGTWFWNRYPGARFDSESYTYAYLFSQELFEEWEWSEHFAE"
BASE COUNT          253 a          492 c          530 g          290 t
ORIGIN      
        1 cgagatcgcc gatgcgctcc gccgtctcgc cgccgtgtga gccgcgccgc gcgcacgcca
       61 gcgggcgatg tgcacgccag ccggctatgc gcctggcgtg agggtgcggc cgatcaccat
      121 gtgttggatc tcgttggtgc cttcgccgat ttcgagcacc ttggcgtcgc agtagaagcg
      181 ggcgacgggc gattcgagca tgtagccgag cccgccgtgg atctgcacgg cctccgatgc
      241 cgccgacatg gccaggcgac tcgccttgag tttcgccatc gacgcctctt tcaggaacgg
      301 cttgccggcg tcgcgtaacg ccgccgcccg gtaggtcagc caccgggccg cttcgagttc
      361 ggtggcgatg tcggcgagct tgaactgaat ggcctggaac ttggcgatcg gctggccgaa
      421 ttgttggcgt tgatgtgcgt agtcgacggc catgtccaac accgcctgcg tgaggctgag
      481 tgacagggcg gcgatggaga tgcgtccgac ctcgagggtg ctgaggaact ggctgagccc
      541 catctcgggg tcgccgatga ggttctcctc gggcacgaac acgtcgtcga agaacaactc
      601 tcgggtgtcg agtcccttcc agccgatgcc gcgcatcttg ggtcccatcg tgaaccccga
      661 agtgtccttc tcaacgacga aactcgcgaa cttcgcgttg tcgtcggagc cggtgcgtgc
      721 caacagggtc accccgaagc tcatgtcggt gccggcgttg gagatgaacg tcttggcgcc
      781 gttgatgagc cagcctccat cgacacgttc ggcacgggtg cggataccgc gggcgtcgga
      841 accggcatcg ggttcggtca ggccgaacgc cccgagcgcg gtgccttcgg cgaggcggcg
      901 taaccaccgt tcgcgctggt cgtcgttgcc gaacaacagc agcggcaacg agccgatcgt
      961 gacgtgggcc tgccaggccg cggcgaccga ctggtctgcg tggccgatct gttccatcgc
     1021 tgcgacgaac ccgaccgtgg tcatctcgat gccgccccag cgttcgggaa tcaacattcc
     1081 gagcaggccg agttcgccca tttcacgcag gagatcggtg ggacacaccg ccgcctccca
     1141 ggcctcgggt gcccgtgggg cgatctcgtt gcgtgcgaaa tcccggcaca gatgtgcaag
     1201 ttcgcgttct gcttccgtca gctcgaagtc cacatttgta tactaaccgg tcggtacaaa
     1261 aagttcgggg gaatggctgt gccgggggac gcgacatgca accaaacgat cttgaaccgt
     1321 ccgaccgagg tgtcggtgtc gacgtgctca tcgtcggcgc cggggtcacg ggcatctacc
     1381 agctctaccg ggcactcgag tgcgggttca gcgccgagat cgtcgaggcc ggctccgggg
     1441 tcggcgggac gtggttctgg aaccgctacc cgggcgcccg gttcgactcg gagagctaca
     1501 cctatgccta cctgttctcg caggaactgt tcgaggagtg ggagtggagc gagcatttcg
     1561 ccgag
//
LOCUS       DAOOMA010000640         1555 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_104488_length_1555_cov_3.402667, whole genome shotgun
            sequence.
ACCESSION   DAOOMA010000640 DAOOMA010000000
VERSION     DAOOMA010000640.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 1555)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1555)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1555
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_104488_length_1555_cov_3.402667"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..821
                     /locus_tag="PLP95_13435"
     CDS             <1..821
                     /locus_tag="PLP95_13435"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HPB46853.1"
                     /translation="PDAPTVVLLHGWVASGGLNWFNAFAPLSQHFRVIAPDFRGHGRG
                     IKNHRRFTLAACADDTAALCREIGVERAIFCGYSMGGPIGQLIWKRHRDLVEGLVFSA
                     TSSMFVPGLQQRLLFASSMALLAGTTRGTQVVTRLPWVVRSHFPKGLREVQTGRPSRI
                     QIWAAQEFRRHDMRLVLEAGTAIATFSSRRWIHEVDVPTTVLLTTKDRAVAPTAQLHL
                     ALAIDGAKIQRFDEGHTSPVLESFGDAITGACLSVEQSIRRKRRSSDRSTKRSN"
     gene            833..>1555
                     /locus_tag="PLP95_13440"
     CDS             833..>1555
                     /locus_tag="PLP95_13440"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HPB46854.1"
                     /translation="MATELHTYNLADVWEAVSDRVPERVAVISEGRDVTYGELEERSN
                     RLAHALYEHGVRAGDHIGAYLVNGIEYFEVMLAAYKLRAVPVNINYRYVKTELEYLFN
                     NSDAVAVVANEEFVERLVETAGAIPKVHTVLIVREGDSAGDLPEGFVDYEEAIAGASA
                     DRDFIELVGPRTNDDEYVIYTGGTTGMPKGVVWRHEDIFFAGIGGGDPMRLNGFVSSP
                     AELIDRIIDFEMLTLPLAPMMHA"
BASE COUNT          281 a          473 c          498 g          303 t
ORIGIN      
        1 ccccggatgc tcctacggtg gtgttgttgc acggatgggt cgcgtccggt gggctgaact
       61 ggttcaacgc cttcgcaccg ctcagtcagc attttcgtgt tatcgcaccg gactttcgtg
      121 gacacgggcg cggaatcaag aatcaccgca ggttcaccct ggcggcgtgt gccgacgata
      181 ccgcagcgtt gtgccgcgaa atcggcgtcg aacgcgccat cttctgtggc tactcgatgg
      241 gcgggccgat cgggcagctc atctggaaac gccatcgaga cctcgtcgag gggctggtgt
      301 tctctgcgac gagttcgatg ttcgtgccgg gccttcagca acgcctgctg ttcgcctcat
      361 cgatggcatt gctcgccggt accacccgcg gtacccaggt ggtcaccagg cttccgtggg
      421 tggtccgctc gcatttcccc aagggcctcc gcgaggtcca gaccggacgg ccatcgcgaa
      481 ttcagatctg ggccgcgcag gagtttcgcc gacacgacat gcgcttggtg ctcgaggcgg
      541 gaaccgctat cgctacgttc tcgtcgcggc gttggattca cgaagtcgat gtgccgacca
      601 cggtgttgtt gacgaccaag gaccgggcgg tcgccccgac ggcgcaattg cacctagcgt
      661 tggcgatcga cggtgcgaag atccagcgtt tcgacgaggg ccacacgtcg ccggtgcttg
      721 aatcgttcgg agatgcaatt accggagcct gcctgagcgt cgaacagtcg attcgacgca
      781 agcgccggtc ctcggaccgg tcgacgaaga ggagcaactg atcgccatgg ccatggccac
      841 tgaactccac acctacaacc ttgctgatgt ctgggaggcg gtgtcggacc gagtgcccga
      901 acgcgtcgca gtcatcagcg aaggccgcga tgtcacctac ggcgagctcg aagagcgctc
      961 aaatcgtctc gcccacgcgt tgtatgaaca cggtgtccgc gccggtgatc acatcggggc
     1021 gtatctcgtc aacggcatcg agtacttcga ggtgatgctc gccgcgtaca agctccgggc
     1081 ggtaccggtg aatatcaact accgctacgt caagacggag ttggagtacc tgttcaacaa
     1141 ctccgacgcg gtggccgtcg tggcgaatga ggagttcgtc gaacgcctcg tggagaccgc
     1201 cggggcgata ccgaaggtgc acacggtgct gatcgttcgc gagggcgact cggcaggcga
     1261 tctccccgag ggtttcgtcg actacgagga ggcgatagcc ggggcctccg ccgaccgcga
     1321 cttcatcgaa cttgtcgggc cccgaaccaa cgacgacgag tacgtgatct acaccggcgg
     1381 gaccaccggc atgcccaagg gtgtcgtgtg gcgccacgag gacattttct ttgccgggat
     1441 cggtggtgga gatccgatgc ggctcaacgg gtttgtgtcc tcgccggcag aactcatcga
     1501 ccgcatcatc gactttgaaa tgttgacgct tccactggct ccgatgatgc atgca
//
LOCUS       DAOOMA010000641         1554 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_104596_length_1554_cov_3.621081, whole genome shotgun
            sequence.
ACCESSION   DAOOMA010000641 DAOOMA010000000
VERSION     DAOOMA010000641.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 1554)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1554)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1554
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_104596_length_1554_cov_3.621081"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..931
                     /locus_tag="PLP95_13445"
     CDS             <1..931
                     /locus_tag="PLP95_13445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB46855.1"
                     /translation="HLVVQHRVVLDGVLEPGLLSRVRKFTVLEQVGHFEEVAVIGKLL
                     DGVIDLVPPPAPRPTTTKGETRALDDPFSGFVFKVAANMDPSHRDRIAFFRVCSGRFE
                     RGMVVTHQRTGKPFATKYAHQVFGQDRETVEEAFPGDVVGLVNANEVRVGDTLFVSPP
                     VEFPRIPAFAPEHFSIARVKDTGRYKQFRKGISQLDEEGVVQVLRNEMRGDQAPILAA
                     VGPMQFEVVAYRLQSEFGAEVELEPTAYRVCRLTDDDGRERLKSMRGVEVLERSDGAL
                     LALFESTYWLDRLVAEEPDLRLDKLVAEGELHQ"
     gene            995..1288
                     /locus_tag="PLP95_13450"
     CDS             995..1288
                     /locus_tag="PLP95_13450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB46856.1"
                     /translation="MTEGDGRSSNDSNPHRLRRIIAVPAIVVELGLIVIGIWQIAGGD
                     DSAPEVKVGGQQTSRDDVDTTATTNVVVGESGELDVEGDPGQVGLVPAQTEER"
     gene            1378..1454
                     /locus_tag="PLP95_13455"
     tRNA            1378..1454
                     /locus_tag="PLP95_13455"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1412..1414,aa:Ala,seq:tgc)
BASE COUNT          276 a          482 c          514 g          282 t
ORIGIN      
        1 tcatctggtc gtgcagcatc gtgtggttct tgatggtgtt ctcgaacccg gtcttctgag
       61 ccgcgttcgg aagttcaccg ttcttgagca ggtaggccac ttcgaggaag ttgcagttat
      121 cggcaagttg ctcgatgggg tgatcgatct ggttcctccg cccgcccccc ggccgacgac
      181 gaccaagggt gagacccggg cactcgacga cccgttctcc gggttcgtgt tcaaggttgc
      241 ggccaacatg gatccatcgc atcgggaccg catcgcgttc ttccgggtgt gttcgggacg
      301 tttcgagcgc ggcatggtcg tcacccatca gcgcaccggc aaaccgttcg ccaccaagta
      361 cgcacaccag gttttcggac aggatcgcga gacggtcgag gaggcattcc ccggagacgt
      421 cgtcggcctc gtcaacgcga acgaggtgcg cgtgggcgac acgctgttcg tcagcccgcc
      481 agtcgagttt ccgaggattc cggcgtttgc tcccgaacac ttctcgatcg cccgagtgaa
      541 ggacacgggt cgttacaagc agttccgcaa gggcatcagc cagctcgacg aggaaggtgt
      601 cgtgcaggtg ctacgcaacg aaatgcgcgg cgatcaagca ccgattctcg ccgcggtcgg
      661 gccgatgcag ttcgaagtgg tggcgtaccg actccagtcg gagttcgggg ccgaggtcga
      721 actcgagccg acggcgtacc gcgtgtgccg gctcaccgac gacgacgggc gcgaacggct
      781 caagtccatg cgcggggtcg aggtgctcga acgttccgac ggcgccctgt tggcactgtt
      841 cgaatcgacc tactggctcg atcgcctcgt cgccgaggag ccggaccttc ggctcgacaa
      901 gctcgtcgcg gaaggcgagt tgcaccagtg atcgggcgcc gaccgatggc cgcactacgc
      961 tcgaccacgg cttgatcgag gagacaatgc gttggtgacc gagggcgacg gccgaagttc
     1021 aaacgattcc aacccgcacc ggctgcggcg gatcatcgct gttcccgcaa tcgtcgtaga
     1081 gctgggactg atcgtcatcg gaatctggca gatcgctgga ggtgacgact ccgctcccga
     1141 ggtgaaggtc ggtggccaac agacctcccg cgacgatgtc gacaccaccg cgaccaccaa
     1201 cgtggttgtt ggcgagtcgg gtgaactcga cgttgaaggc gaccccgggc aggtcgggct
     1261 cgtccccgcg cagaccgagg aacgctaggc cctcgggcgc ggtgggcgcc ggctctgggc
     1321 gggctggcac ctgattaccc agggagcgcg gtgcacgcct agaatcaagg atcccttggg
     1381 ggtgtagctc agttggctag agcacctgct ttgcaagcag ggggtcgtgg gttcgagtcc
     1441 catctcctcc accagatcgt cgcaataccg accgccgtca gcaccgtcat gacggcgagc
     1501 cccgacgctg tcgacgcatt cgccggagat ccagagagct tgcccaagtt ggcg
//
LOCUS       DAOOMA010000642         1551 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_104902_length_1551_cov_2.831551, whole genome shotgun
            sequence.
ACCESSION   DAOOMA010000642 DAOOMA010000000
VERSION     DAOOMA010000642.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 1551)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1551)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1551
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_104902_length_1551_cov_2.831551"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1528)
                     /locus_tag="PLP95_13460"
     CDS             complement(<1..1528)
                     /locus_tag="PLP95_13460"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HPB46857.1"
                     /translation="MLPAAWLTIAVTVVLASALSAFGSQDRGDAIASLLNVANWRFLA
                     TGSSYNDLFSTPSPFRHFWSLAIEEQAYVALPVLVWVALRVSRGSLSVLASVLGVLLS
                     LSMWTTLTADSVDRVYYGTITRSAEILIGALLAAMVAHPKTRVHLLRRWVLRSVVVVA
                     GVVSLVGFLVACVRVEVGSAFVLKGGLVLIAVGSAALILNAAMGIGIIGFIFEWRPLR
                     WLGGISYGVYLFHWPLFTLLTERRTGLGHFPRFVVLVALTLVLAVLSSSHVEIPMRRR
                     RFPKPALPTMASAAAVIVVVLALIMPTTTSLVFDAEAASAQLDTLVMGSLPAATSGES
                     GTSADGSPGEGLSELAAAQAQREAERAPAIMHFGDSVALSMAFPLAAWAVETQGGFFL
                     GGDTTIGCGIGRGGYERSFGVVPRRAECDNWPQRWYPITDVDGLDIAMVHTGQWELVD
                     RRIPLGMNHTVVGEDAALLSTPVPTQIDEVWRNVGDPIYDDYLLAELSAAQELLSRRG
                     A"
BASE COUNT          319 a          529 c          494 g          209 t
ORIGIN      
        1 gcgctccgcg acgcgacagc aactcttgag ctgccgacag ctctgcgagc aggtagtcgt
       61 cgtagatcgg atctccgacg ttgcgccaca cctcatcgat ctgcgttggc acgggggtgc
      121 ttagcagcgc ggcgtcttcg ccgacgacgg tgtgattcat cccgagtggg atacgacggt
      181 cgaccagttc ccactgtccc gtgtgcacca tggcgatgtc gagaccgtcg acgtcggtga
      241 tcggatacca gcgctgcggc cagttgtcgc actcggcacg acgaggaacc accccgaagg
      301 agcgttcgta gccgccgcga ccgatgccac acccgatcgt ggtgtcgcct ccgaggaaga
      361 acccaccctg ggtttccacc gcccaggcgg ccagcgggaa cgccatgctg agggccaccg
      421 aatcgccgaa gtgcatgatc gccggtgcgc gttcggcctc gcgttgcgcc tgggccgcgg
      481 ccaactcgga caacccttcg cccggcgaac cgtcggcgga ggttccggac tcccctgacg
      541 tcgctgccgg aaggctgccc atgacgaggg tgtcgagttg agccgaagcg gcctcggcat
      601 cgaacaccag cgaggtggtg gtgggcatga tcaacgccaa caccacgacg atgaccgccg
      661 cggccgacgc catcgtcggc agggcgggtt tcgggaatcg acgacgtcgc atcggaatct
      721 cgacgtgact gctgctgagg accgccagca ccagcgtcag cgccacgagc acgacgaacc
      781 gcgggaaatg gccgagtccg gtgcggcgtt cggtcagcag cgtgaacagt ggccagtgga
      841 acaggtacac gccgtaggag attccgccga gccaccgcag cggtcgccat tcgaagatga
      901 acccgatgat gccgatgccc atcgcggcgt tcaggatcaa cgccgccgat ccaacggcga
      961 tcagcaccag accgcccttg aggacgaacg cacttccgac ctcgacccgg acgcaggcca
     1021 ccaggaaacc caccaacgac acgaccccgg ccaccacgac gacgcttcgc agcacccatc
     1081 gtcgcaacag atgcaccctg gtcttggggt gggcgaccat cgctgccagc agtgcgccga
     1141 tgaggatctc ggccgagcgg gtgatggtgc cgtaatagac gcgatcgaca gagtcagcgg
     1201 tcagcgtcgt ccacatcgac agcgacaaga gcaccccgag aacgctcgcc agcacgctca
     1261 gcgagcctct cgagacccgc agagccaccc acaccagcac cggcagcgcc acatacgcct
     1321 gttcctcgat tgcgaggctc cagaagtgcc gaaacggaga cggcgtggaa aacaggtcgt
     1381 tgtaggacga cccggtcgcc aggaaccgcc agttggcaac gttcaacaac gaagcgatcg
     1441 cgtcgccgcg atcctgtgaa ccgaacgccg acagcgccga ggccaacacg acggtgaccg
     1501 cgatggtgag ccaggcggcc gggagcaacc gccggaaacg ccgagcccag a
//
LOCUS       DAOOMA010000643         1521 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_108038_length_1521_cov_3.182810, whole genome shotgun
            sequence.
ACCESSION   DAOOMA010000643 DAOOMA010000000
VERSION     DAOOMA010000643.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 1521)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1521)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1521
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_108038_length_1521_cov_3.182810"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..681)
                     /gene="aroC"
                     /locus_tag="PLP95_13465"
     CDS             complement(<1..681)
                     /gene="aroC"
                     /locus_tag="PLP95_13465"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HPB46858.1"
                     /translation="MSSQSGRLFRVSTYGESHGVAVGVVVDGCPPRLELCEADIQADL
                     DRRRPGQSRLVTQRNEADVVEILSGMWEGRTTGTPIALEVRNADHRSSAYDHMADLYR
                     PSHADYTYDAKYGIRAIAGGGRASARETIGRVAAGAVARKLLKVAAGIEVLAWVSSVH
                     SIDADEAIDATSVTLEQVEADPTRCPSPLHAARITEAIEAARRDGDSLGGVVTCVARN
                     VPAGLGEPV"
     gene            697..>1521
                     /locus_tag="PLP95_13470"
     CDS             697..>1521
                     /locus_tag="PLP95_13470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB46859.1"
                     /translation="MDADWFMMSSALPTMAALRTRMFDRQVSRVWPHALALFALLMAC
                     SFLVSNGRISNADEGAVLSQLEIIDTYGSWMMPNPLPSIDPDGSWFGIENSDLGATGF
                     LTYGKHPMYPTAMAPFYAAFGYHGLLSASVLGTVATAWAAAALARRWRPSAAVATLWV
                     VGVATPIFFDSFWAMAHTIGAAFATVSLLGAIAVVVDRRRIHLVTTVFGVAGAALFRS
                     EGVLWGLALGVAVALWGSLDAWRRARAEAPGTSPRASVLRWVRRLDPFAVATSALAG"
BASE COUNT          187 a          512 c          545 g          277 t
ORIGIN      
        1 aaccggctcg ccgaggccgg cgggcacgtt acgggcgacg caggtgacca cgccgccgag
       61 cgagtctccg tcccggcgcg ccgcttcgat cgcctcggtg atcctcgccg cgtgcagcgg
      121 cgacgggcat cgcgtcgggt cggcctcgac ctgttcgagg gtcacactcg tcgcgtcgat
      181 cgcctcgtcg gcgtcgatcg aatgcaccga actcacccac gcgagcacct cgatgccggc
      241 ggcgaccttc agcagctttc gggcgacggc tccggcggcg acccggccga tggtctcgcg
      301 cgccgaggcc cgtccgccgc cggcgatcgc gcggatgccg tacttggcgt cgtaggtgta
      361 gtcggcgtgg ctcggccggt acaggtcggc catgtggtca taggcgctcg aacggtgatc
      421 ggcgttgcgg acctcgagcg cgatgggggt gccggtggtg cggccctccc acatgcccga
      481 gagaatctcg acgacgtctg cctcgttgcg ttgggtgacc agtcggctct gcccgggtcg
      541 acgacggtcg aggtcggcct gaatgtcggc ctcgcacagt tcgagccggg gagggcagcc
      601 gtcgacgacg accccgaccg ccaccccatg ggattcgccg taggtggaca cccgaaacag
      661 ccggccgctc tgcgaagaca tgctcacaag tatggcgtgg acgccgattg gttcatgatg
      721 agttccgccc tgccgacgat ggccgcgctt cgcacgcgca tgttcgatcg acaggtctcg
      781 cgagtgtggc cccacgcgtt ggccttgttc gccctgttga tggcctgttc gtttctcgtg
      841 tcgaacggca ggatctccaa cgccgatgag ggggcggtgc tgtcccagct cgagatcatc
      901 gacacctacg gaagctggat gatgcccaat cctcttccat cgatcgaccc cgacggttcc
      961 tggttcggca tcgagaactc ggacctcggc gccaccgggt tcctcaccta cggcaaacac
     1021 cccatgtacc cgacggcgat ggcgccgttc tacgccgcgt tcggctatca cggcctgttg
     1081 agcgcctcgg tgctcggcac cgtggcgacg gcgtgggcgg ccgccgcgtt ggcccggcgt
     1141 tggcgcccga gcgctgcggt ggcgacgctg tgggtggtcg gggtggcgac gccgatcttc
     1201 ttcgattcct tctgggcgat ggcccacacc atcggcgccg cctttgccac ggtgtcgcta
     1261 ctcggcgcga tcgccgtggt cgtcgatcgt cgccggatcc acctcgtcac caccgtgttc
     1321 ggcgtggcgg gagcggcgtt gttccggagc gagggggtgc tgtggggtct cgcgctcggc
     1381 gtggcggtgg cgctgtgggg gtcgttggac gcgtggcgcc gggctcgtgc ggaggccccc
     1441 ggtacgtcgc cgagggcgtc ggttctccgg tgggttcgcc gtctcgaccc gttcgcggtg
     1501 gcgacgagcg cactcgcggg t
//
LOCUS       DAOOMA010000644         1514 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_108814_length_1514_cov_3.352981, whole genome shotgun
            sequence.
ACCESSION   DAOOMA010000644 DAOOMA010000000
VERSION     DAOOMA010000644.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 1514)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1514)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1514
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_108814_length_1514_cov_3.352981"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..911)
                     /locus_tag="PLP95_13475"
     CDS             complement(<1..911)
                     /locus_tag="PLP95_13475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012229346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HPB46860.1"
                     /translation="MNSESDPVAPTAEPAAPSGEVVIIGAGPAGLTAAYQLGKVQRHA
                     TVLESDDIVGGISRTVERNGWRFDIGGHRFFTKVPEVSALWHEILPQGDFLLRPRKSR
                     IFYNGKYFDYPLKAMNALTNLGPIEAIKCVASYAVAQVRPPKDQDNYEGWLVARFGWR
                     LYRTFFKTYTEKVWGVPVSSMPADWAAQRIKNLSLFNAIVNALLPNRNQKDIASLIEE
                     FEYPKFGPGMMWERCTDLVTEQGGDVIMQTRVVEIRHSDGVAHTVVAESTDGARTEYP
                     AEHVISSMPMPALLRSITRNGANPADE"
     gene            complement(930..1172)
                     /locus_tag="PLP95_13480"
     CDS             complement(930..1172)
                     /locus_tag="PLP95_13480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB46861.1"
                     /translation="MAFEITLTDHHTEVVADADTFAHDKTMTTFYRTGNARQTIDCWS
                     VPVASIRTDNILMVRMVAGADVACEPIASVHHLATA"
     gene            complement(1320..>1514)
                     /locus_tag="PLP95_13485"
     CDS             complement(1320..>1514)
                     /locus_tag="PLP95_13485"
                     /inference="COORDINATES: protein motif:HMM:NF024591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HPB46862.1"
                     /translation="RGSEVVAVVSLRPDATVSDDELIAHVKARLASYKAPKSIVRVAG
                     VRRSPSGKPDYAWARSLAEG"
BASE COUNT          262 a          449 c          520 g          283 t
ORIGIN      
        1 cactcgtcag ccggattggc gccgttgcgg gtgatcgagc gcagcagcgc cggcatgggc
       61 atcgagctga tgacgtgttc ggcggggtac tcggtgcggg cgccgtcggt cgattccgcg
      121 accacggtgt gggcgacacc gtcgctatgg cgaatttcca ccactcgggt ctgcatgatc
      181 acgtcgccac cctgttcggt gaccagatcg gtgcaacgct cccacatcat gcccgggccg
      241 aacttcgggt actcgaactc ttcgatgagc gaggcgatgt ctttttggtt ccggttcggc
      301 aacagcgcgt tcacgatggc attgaacagc gacaggttct tgatgcgttg cgcagcccag
      361 tcggcgggca tggaactgac cggaaccccc cacacctttt cggtataggt cttgaagaac
      421 gtgcggtaga ggcgccaacc gaaccgtgcg acgagccacc cctcgtagtt gtcctgatcc
      481 ttcggtgggc ggacctgagc gacggcatag gaggcgacgc acttgatcgc ctcgatcggc
      541 ccgaggttcg tcaacgcgtt catcgccttg agcggataat cgaagtactt gccgttgtag
      601 aaaatccgac tcttgcgcgg ccgcaacagg aagtcgccct ggggaaggat ctcgtgccac
      661 agggccgaca cctcaggaac cttggtgaag aaacggtgac caccgatgtc gaaacgccat
      721 ccgttgcgtt cgaccgtgcg ggagatgcca ccgacgatgt cgtcgctttc cagcacggtg
      781 gcgtgacgtt gaaccttgcc gagctgatag gcagcggtga ggccggctgg cccggcgccg
      841 atgatcacga cctctcccga tggcgcggcg ggttcggcgg tgggggcgac ggggtcggac
      901 tcagaattca cgagagccga tgctagttct cacgccgtgg cgagatggtg aacgctcgct
      961 atcggttcac aggcgacatc ggccccggca accatgcgaa ccatcaagat gttgtcggtg
     1021 cggatactcg cgacgggaac cgaccagcag tcgatcgtct ggcgagcgtt accggtgcgg
     1081 taaaaggtgg tcatggtctt gtcgtgggcg aaggtgtcgg cgtcggcgac gacctcggtg
     1141 tgatggtcgg tgagggtgat ttcgaaagcc atgtaggcat cgtgcaccct caggcctgtg
     1201 gggaaccagg gcagcatcgt tgggtctttc ctgggtgcgg actgtggaca cggccccttg
     1261 gcggtggatg cccggtggat gcccggtgga tgcccggtgg aggtggcgga ggcggcgtct
     1321 cagccctcgg ccagcgaacg cgcccaggcg tagtcgggct tgcccgaagg gcttcgtcga
     1381 acgccggcga cccggacgat cgacttcggg gccttgtagg aggcgaggcg cgccttgacg
     1441 tgagcgatga gctcgtcgtc gctcaccgtc gcgtcgggtc gcaacgacac caccgccacc
     1501 acctccgaac cccg
//
LOCUS       DAOOMA010000645         1504 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_110023_length_1504_cov_2.677019, whole genome shotgun
            sequence.
ACCESSION   DAOOMA010000645 DAOOMA010000000
VERSION     DAOOMA010000645.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 1504)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1504)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1504
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_110023_length_1504_cov_2.677019"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..546)
                     /locus_tag="PLP95_13490"
     CDS             complement(<1..546)
                     /locus_tag="PLP95_13490"
                     /inference="COORDINATES: protein motif:HMM:NF020892.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HPB46863.1"
                     /translation="MTRYLITSALPYINGVKHLGNLVGSMLPADASARFLRMSGEEVL
                     FICATDEHGTPAEIAAAEAGLPVEEFCRQAHEVQKGLGERFLLSFDHFGRTSRPETHE
                     ATRHIAAKLEEQGYLEERVIKQVYSVADGRFLPDRYVKGICPNCGSPDQYGDNCEVCG
                     ATYAPTELKEPKSVVSGATPVL"
     gene            642..1289
                     /locus_tag="PLP95_13495"
     CDS             642..1289
                     /locus_tag="PLP95_13495"
                     /inference="COORDINATES: protein motif:HMM:NF015089.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HPB46864.1"
                     /translation="MITELVYGFDIETDTTTDGLDPNVSSVVAAAVVSARERLVFVGD
                     EATLLRDLDAALAEMAPGVLVTWNGLRFDLPFIALRARMRRLDLGLRLDPEPLEWRRD
                     DEARGSAAPRTSRRVRWGPPSPAASWGAHRHLDAYRLYRGDVGQTLGLSCALKPLSRF
                     LGYEPVEVDRSRIDLLTPTELDAYVSSDAELARELALRRPAALAGWCQRNTKIEW"
BASE COUNT          247 a          501 c          482 g          274 t
ORIGIN      
        1 cagcaccggc gtcgcgccgg acacgaccga cttcggctcc ttgagctcgg tgggggcata
       61 ggtggcgccg cagacctcgc agttgtcgcc gtactggtcg ggcgatccgc agttggggca
      121 gatgcccttc acgtagcgat cgggcaggaa ccgaccatcg gcaaccgaat acacctgttt
      181 gatgacgcgc tcttcgaggt acccctgctc ctcgagtttg gcggcgatgt gacgggtcgc
      241 ctcgtgagtc tcggggcgac tcgtgcgccc gaagtgatcg aagctcaaca gaaaacgctc
      301 gccgagaccc ttttgcacct cgtgggcctg ccggcagaac tcctccaccg gcaatccggc
      361 ctcggccgcg gcgatctcgg cgggggtgcc gtgttcgtcg gtcgcacaga tgaacagcac
      421 ctcttccccg ctcatccgca ggaacctcgc ggacgcgtcg gccggaagca tcgaaccgac
      481 gagattgccg aggtgcttca caccgttgat gtacggaagg gccgaggtga tgagatagcg
      541 agtcatggat gtcccgtggt cgtgggcgtc cagcgacgca ccgatcccgc gatcctacga
      601 gttcgacgag agtgtcggtg cctcgtcgta gcatcggcgc gatgatcacc gagctcgtct
      661 acggcttcga tatcgagacc gacaccacca ccgacggcct cgacccgaac gtctcgagcg
      721 tcgtcgccgc ggccgtggtg tccgcacgcg agcggctcgt gtttgtcggc gatgaggcga
      781 cgctgctgcg cgatctcgac gctgcgctcg ccgagatggc tcccggcgtg ctggtcacct
      841 ggaacggact gcgtttcgac ctgccgttca tcgcgttgcg cgctcgaatg cggcggctcg
      901 acctcgggct tcgcctcgac cccgaaccgc tcgagtggcg ccgcgacgac gaggcccgcg
      961 gctccgcggc ccctcgcacc tcgcggcgtg tgagatgggg gccgccctcc cccgccgcct
     1021 cctggggggc gcatcgccac ctcgacgcct atcgcctcta ccgtggcgac gtcggccaga
     1081 cgctcgggct gtcgtgtgca ctcaagccac tgtcgcgatt tctcggatac gaaccggtcg
     1141 aggtggatcg atcgcggatc gaccttctca ccccgacgga actcgacgcg tacgtctcca
     1201 gcgacgccga actcgcacgc gagcttgcgc tgcgacgacc tgcggcgctc gccggttggt
     1261 gccagcgcaa cacgaaaatc gagtggtgag ctgtccaagt ggcaccgcgt ctctctcaaa
     1321 ctctaaatcc accacgcggc atctcagtat ttccgagtgc atctttgacg cgatgacaaa
     1381 agtcacataa cttgcagatc ttgttggcat gcccggtggc tccgggtagc gacggaggga
     1441 taactgaatg aagcgaacgc gcctgttggc tctgactggg ctggtctcgg caaccgtgat
     1501 ggtg
//
LOCUS       DAOOMA010000646         1497 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_110859_length_1497_cov_1.739945, whole genome shotgun
            sequence.
ACCESSION   DAOOMA010000646 DAOOMA010000000
VERSION     DAOOMA010000646.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 1497)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1497)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1497
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_110859_length_1497_cov_1.739945"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            147..1091
                     /locus_tag="PLP95_13500"
     CDS             147..1091
                     /locus_tag="PLP95_13500"
                     /inference="COORDINATES: protein motif:HMM:NF021916.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_function: GO:0004476 - mannose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HPB46865.1"
                     /translation="MPGAAILDSEGAYAVAQSTPSAVRATLAAAVRAEVPNDLPIRAV
                     VLASSARSRLTSSAVIAIAAERASVPVVELTAGPLPRWVGSDTLVVALDSGVAEGLLS
                     DSTVDGVPIVAVGGNVDDRPQPIVETAAALAALEQFGVVSGLLDDMVAACGFLEARWA
                     ELVSSPSAAQRLAKRVGRTIPLVYGAGAVGHAATAAWKHSVNRNAKAPAFANAVPGVD
                     HDEVCGWAQHGDVTRQVFTLVVLRHPFQSADESHRLDVTAETCDEIVAGVHQVTVSSP
                     NKVAALFELMLHGEVVSLEMAALADIDPGPTPITDRYL"
     gene            1281..>1497
                     /locus_tag="PLP95_13505"
     CDS             1281..>1497
                     /locus_tag="PLP95_13505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018036870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HPB46866.1"
                     /translation="MDFKVADLSLAGFGRKEIELAEHEMPGLMATRAEFADAQPLKGA
                     RIMGSLHMTVQTAVLIETLVALGADVRW"
BASE COUNT          222 a          496 c          510 g          269 t
ORIGIN      
        1 gcccatctgg gattcttcga ctggatgcga tttggcatcc cggtgatgct gatcgacgag
       61 gctgagtcgg tctctgagga ggagcacgcg cgcttgctgg cgaaggccga ggccgacggt
      121 atccagctca acttcacacc ggcctgatgc ccggcgccgc gatcctcgac agtgagggcg
      181 cgtacgcagt ggcgcagtcg acgccatcgg cggttcgggc caccctcgct gcggcagtcc
      241 gcgccgaggt gcctaacgat cttccgattc gcgctgtggt gctggcgtcg tccgcccgtt
      301 cacggctcac ctcctcggcg gtcatcgcga tcgccgccga gcgcgcctcc gtccccgtcg
      361 tcgagttgac cgccggtccg ttgccccggt gggtcggcag tgacaccctt gtggtcgcgc
      421 tcgactcagg cgtcgccgag ggactgttga gcgactcgac cgtcgacggc gtgcccatcg
      481 tcgcggtagg cggcaatgtt gacgatcgcc cgcaacccat cgtcgagacg gccgctgcgc
      541 tcgcggcgtt ggagcagttc ggcgtcgttt cgggactact cgacgacatg gtcgcggcgt
      601 gcgggttcct tgaagctcgg tgggccgaac tggtgtcgtc accgtcggcg gcacagcggc
      661 tcgcgaagcg ggtcggtcgc accattcccc tggtctacgg cgctggtgct gtcggccacg
      721 cggccacggc ggcctggaaa cactcggtca accgcaacgc caaagccccg gcgttcgcga
      781 acgcggtgcc gggcgtcgac cacgatgagg tgtgcggttg ggcccagcat ggcgacgtca
      841 cccggcaggt gttcacgctc gtggtgctcc gtcacccgtt ccagtctgct gatgagtcgc
      901 accgtctcga cgtgacggcc gagacgtgtg acgagatcgt cgcgggcgtt caccaggtga
      961 cggtgtcgtc gcccaacaag gtggctgcgc tgttcgagtt gatgctgcac ggcgaggtgg
     1021 tttcgttgga gatggccgcg ctcgcagaca tcgatccggg cccgacgccg atcaccgacc
     1081 gctacctctg agcggagctt gacgacacag cgggcgggcc actgcacgcg gcccgtcgat
     1141 cgcctacgct gtccggccgt acgggctgct gtgaacccgg tgtctgatga ccccagccac
     1201 gcacactcat ccgagcagtg acgcgacgcg ccgaaaccgg ttgcggtgtc gctgacatga
     1261 cgtcgcggag gcgcaaccac atggacttca aagtcgcaga tctgtccctt gccggattcg
     1321 gtcgtaagga aatcgagctc gccgagcacg agatgcccgg gctcatggcc accagggccg
     1381 agttcgccga tgcgcagccg ctcaagggcg cccgcatcat gggttcgctc cacatgacgg
     1441 tgcagacagc ggttcttatc gaaacgctcg tcgccctggg tgccgacgtt cgttggg
//
LOCUS       DAOOMA010000647         1491 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_111495_length_1491_cov_2.571031, whole genome shotgun
            sequence.
ACCESSION   DAOOMA010000647 DAOOMA010000000
VERSION     DAOOMA010000647.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 1491)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1491)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1491
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_111495_length_1491_cov_2.571031"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1462)
                     /gene="glgB"
                     /locus_tag="PLP95_13510"
     CDS             complement(<1..1462)
                     /gene="glgB"
                     /locus_tag="PLP95_13510"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: protein motif:HMM:TIGR01515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003844 - 1,4-alpha-glucan branching
                     enzyme activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching enzyme"
                     /protein_id="HPB46867.1"
                     /translation="MARDHVVAELGLSDALLVLVSEQDLTLDDRRFNLPGSVGGTNWR
                     YRSRLTLADLRGDTDLGTEARRVLANLHSWRRTSRGNWPEGSGPAVDVADIESLHRGS
                     HARLADRFGLHPVIARGMIGAAVAVWAPHARSVFVAGDFDGWAGTRLTQHGTSGVWDG
                     FAPSAVLGDRYKLRIEAAAGRWLEKCDPFARSAELPPGNASIVTADDPAIDGPHRTWG
                     DGDWMARRAHHQRAGQPISIYEVHLGSWRFGADGRPLGYREAASQLAAYCRELGFTHV
                     ELMPVMEHPFGGSWGYHVTGFFAPTSRFGTPNDFAAFVDELHQAGIGVIVDWVPAHFP
                     EDAFGLVDFDGEALFEYADPRLGRHPEWGSRVFDYGRNEVRAFLVSSARWWVERYHID
                     GLRVDAVASMLYRNYGREDGEWVPNVHGGRENLEAVAFVRHLNDEIHTAAPGVLMIAE
                     ESTAWAGVTAPTSHGGLGFDLKWDLGWMHDTLDYLQR"
BASE COUNT          255 a          522 c          491 g          223 t
ORIGIN      
        1 cgcgctgcag atagtcgagc gtgtcgtgca tccaccccag gtcccacttg aggtcgaagc
       61 ccagtccgcc gtgcgatgtg ggtgcggtca ccccggccca ggcggtggac tcctcggcga
      121 tcatcaacac gccgggtgcc gcggtgtgga tctcgtcgtt gaggtgacgg acgaacgcga
      181 cggcctcgag gttctcgcgt ccgccatgca cgttcggcac ccattcgccg tcctcgcggc
      241 cgtagttgcg atacagcatc gacgccaccg catcgacccg cagcccgtcg atatggtagc
      301 gctcgaccca ccaacgagcc gacgacacga gaaaggcccg cacctcgttt cggccgtaat
      361 cgaacacccg gctgccccac tcggggtgac gaccgaggcg cggatcagcg tattcgaaca
      421 gcgcctcacc gtcgaagtcg accagcccga acgcatcctc gggaaagtgc gccggcaccc
      481 aatcgacgat cacgccgata cccgcctgat ggagctcgtc gacgaaggct gcgaaatcgt
      541 tcggggtgcc aaaacgcgag gtcggggcga agaacccggt cacgtggtag ccccacgagc
      601 cgccgaaggg gtgttccatc accggcatga gctcgacgtg ggtgaagccc agttcgcggc
      661 agtacgcggc gagttgtgac gccgcctccc gatagccgag cgggcgacca tcggccccga
      721 agcgccacga cccgaggtgc acctcgtaga tcgaaatcgg ctggcccgcc cgctgatgat
      781 gtgcgcgccg agccatccag tcgccatcgc cccaggtccg gtggggaccg tcgatggccg
      841 ggtcgtcggc ggtgacgatc gaggcgttgc cgggcggaag ttccgcgctc cgggcgaacg
      901 ggtcgcattt ttcgagccac cgtccggcgg ccgcctcgat gcgcagcttg tagcgatctc
      961 cgagcaccgc ggatggcgcg aacccgtccc acaccccgga ggtcccgtgt tgggtcagcc
     1021 gcgtgcccgc ccatccgtcg aagtcgccgg caacgaacac cgaacgggca tgcggtgccc
     1081 acaccgccac cgcggcgccg atcatccctc gggcgatgac cgggtgcagc ccgaaacggt
     1141 cggcgagccg tgcgtgtgag ccgcggtgca acgactcgat atcggcgacg tccaccgcgg
     1201 gacccgaccc ttcgggccag ttgccgcgcg aggtgcgcct ccatgaatgg agattggcca
     1261 ggacccgtcg agcttcggtt cccaggtcgg tgtcgcctcg aagatctgcc agggtcagac
     1321 ggctgcgata ccgccagttg gttccgccga ccgatcccgg aaggttgaaa cgacgatcgt
     1381 cgagggtcag gtcctgttcg ctcaccagca ccaacaacgc gtcgctcagg ccgagctccg
     1441 cgacaacgtg atcgcgggcc acgcgcgggt cgatcgcgtc gcccgtcgtg t
//
LOCUS       DAOOMA010000648         1482 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_112536_length_1482_cov_4.336370, whole genome shotgun
            sequence.
ACCESSION   DAOOMA010000648 DAOOMA010000000
VERSION     DAOOMA010000648.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 1482)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1482)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1482
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_112536_length_1482_cov_4.336370"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..323)
                     /locus_tag="PLP95_13515"
     CDS             complement(<1..323)
                     /locus_tag="PLP95_13515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB46868.1"
                     /translation="MDQDSEEPESNRGPDATVSDGMEHLQVAANEMIAAARVFLNVIE
                     EVVADREKLASVAGGVTDLLSGTSEMLTRLVQAAGSTHAADPAGADGASARGRHTTEE
                     SASRRP"
     gene            complement(390..>1482)
                     /locus_tag="PLP95_13520"
     CDS             complement(390..>1482)
                     /locus_tag="PLP95_13520"
                     /inference="COORDINATES: protein motif:HMM:NF014433.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ArsA family ATPase"
                     /protein_id="HPB46869.1"
                     /translation="ACAADGARVTVMSTDPAHSLSDSFGVALDSSLTEVAANVQATEL
                     DAMTRMEENWGEIRSYLRDVLRWAGVDEIEAEELSIIPGLEEIFALTDIVTFDRSGEV
                     DVLIVDCAPTAETIRFLSLPDVLSWYMDRAFPLGRRLTATVGPVVQRLASIPVAGDHV
                     FGAVERMYRELEAVRTLLRDGSRSTARLVVTPEKMVVAEARRTFTYLSLFGYRTDAVI
                     ANRILPDEVTDPWFDEWKRSQSHHLEEISEGFAPLRVLRAPLADREVVGVEALTALGT
                     HLYREVAAAEVLIDGDAVRIDREGETFRVHLDLPFATKDNLQVSRSAAEVFVRVGPYR
                     RSILLPESLRRRDIVRATLSEGRFTIVFE"
BASE COUNT          266 a          527 c          450 g          239 t
ORIGIN      
        1 ggtcggcggc tcgccgattc ctcggtcgtg tgccttccac gggccgaggc gccgtccgcg
       61 cctgccgggt ctgcggcgtg ggtcgacccg gccgcctgaa caaggcgggt cagcatctcc
      121 gacgttccgg acaacagatc ggtcacgcca cccgccaccg aggccaactt ctcgcggtcg
      181 gcgacgacct cctcgatcac gttcaagaag acgcgggcgg cggcgatcat ctcattcgcc
      241 gccacctgca ggtgttccat cccgtcggag acggtcgcgt ccgggcctcg gtttgactcc
      301 ggttcctctg agtcctgatc catcgggtcc atcgggtcca tcatgccaac gagtgaacga
      361 ctcgcgctgc cggcgcccac atcaacgacc tattcgaaca cgatcgtgaa tcggccttcg
      421 gacagcgtcg cccgcacgat gtcacgacgc cgaagcgact ccggcagcag aatcgagcgt
      481 cgataggggc ccacacggac gaacacctcc gcggcgctgc ggctcacctg caggttgtcc
      541 ttggtcgcga acgggaggtc gagatgcact cggaaggtct ccccctcgcg gtcgatccgt
      601 acggcatcgc cgtcgatcag cacctccgcc gcagccacct cgcgatacag gtgcgttccg
      661 agcgcggtga gcgcctcgac gccgaccacc tcgcgatcgg ccagcggggc acgcaacacc
      721 cgtagcggcg caaacccctc cgagatctcc tcgaggtgat gggactgcga acgtttccac
      781 tcgtcgaacc acgggtcggt cacctcgtcg ggcaggatgc gattcgcgat caccgcgtcg
      841 gttcgatagc cgaacaacga cagataggtg aaggttcgac gggcctcggc gaccaccatc
      901 ttttccgggg tcacgacgag gcgagcggtc gatcgcgagc catcgcgcag cagcgttcgg
      961 accgcttcca gctcacggta catccgctcg accgcaccaa agacgtgatc gccggcgaca
     1021 ggaatactcg ccaggcgctg cacgaccggc cccaccgtcg cggtgaggcg ccgaccgagt
     1081 ggaaacgctc ggtccatgta ccaactcaac acgtcgggca acgagagaaa cctgatcgtt
     1141 tcggccgtgg gcgcgcaatc gacgatcagc acgtcgacct cgccgctgcg gtcgaaggtc
     1201 acgatgtcgg tgagcgcgaa aatctcctcg agtcccggga tgatcgacaa ttcctcggcc
     1261 tcgatctcat cgactcccgc ccagcgcaac acgtcgcgca aatagctgcg aatctcgccc
     1321 cagttctcct ccatccgggt catcgcgtcg agttcggtcg cctgcacgtt cgccgcgacc
     1381 tcggtgagcg atgaatcgag agcgacgccg aagctgtccg acagcgagtg ggccgggtcc
     1441 gtcgacatca ccgtgaccct cgcaccatct gccgcgcagg cc
//
LOCUS       DAOOMA010000649         1476 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_113244_length_1476_cov_2.643913, whole genome shotgun
            sequence.
ACCESSION   DAOOMA010000649 DAOOMA010000000
VERSION     DAOOMA010000649.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 1476)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1476)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1476
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_113244_length_1476_cov_2.643913"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1476
                     /locus_tag="PLP95_13525"
     CDS             <1..>1476
                     /locus_tag="PLP95_13525"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020376835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HPB46870.1"
                     /translation="MNPFEHGEVYVTEDGGETDLDLGHYERFIDENLTANSNATTGSI
                     YTAVIAAERRGDYLGKTVQVIPHITDEIKSRIAAMASPDIDVAITEVGGTVGDIEILP
                     FIEAIRQFRLDVGRENCCYIHLTLVPFIGPSGEQKTKPTQHSVTELRSRGIQPQIIVL
                     RSEAPVGDGVKRKISSLSDVPVEAVINAADAESLYDLPGVLHAEGLDTEVVKLLGLDV
                     PEPDMTEWDEVVRRVKTADFPVRIGIVGKYVALPDAYLSVVESLYHAGAFNGSDIRIE
                     WISAEEAEDLVASGEMGNLDGIVIPGGFGERGVEGKIAAARYTREHDIPTLGLCLGLQ
                     VMTIEYARDALGMDGANSAELDPDTPYPVIDIMDDQVDVENKGGTMRLGAYPAVLGPG
                     TRVAEIYGSTEVSERHRHRYEFNNRFRERFEGTDFVISGTSPDGRLVEFIELANHPFW
                     IGTQAHPELKSRPTHPAPLFREFVVAARARACDELRLRVRD"
BASE COUNT          277 a          484 c          479 g          236 t
ORIGIN      
        1 gatgaatccc ttcgaacacg gagaggtgta cgtcaccgag gacgggggtg aaaccgacct
       61 cgacctcggt cactacgagc gcttcatcga cgagaacctg accgccaact ccaacgcgac
      121 gacggggtcg atctacaccg cggtcatcgc ggcggagcgt cgcggcgact atctgggcaa
      181 gaccgtgcag gtgattcctc acatcaccga cgagatcaag tcccgtatcg ccgcgatggc
      241 gagtcccgac atcgacgtgg cgatcaccga ggtcggcgga accgtcggcg atatcgagat
      301 cctgccgttc atcgaggcga tccgacagtt ccgcctcgac gtggggcgcg agaactgctg
      361 ctacatccac ctgaccctgg tgcccttcat cggaccgtcg ggggagcaaa agaccaagcc
      421 gacccagcac tcggtgaccg agctgcgaag ccgcggcatt cagcctcaga tcatcgtgtt
      481 gcgttccgag gcaccggtgg gcgacggcgt gaagcgcaag atcagttcgc tcagcgacgt
      541 cccggtcgaa gcggtcatca acgccgccga tgccgagagc ctctatgatc tgcccggcgt
      601 actgcacgcc gagggcctcg ataccgaggt agtcaagttg ctcggactcg acgtgcccga
      661 accggacatg accgaatggg acgaggtcgt gcggcgtgtg aagaccgcgg atttcccggt
      721 gcgaatcggc atcgtcggca aatacgtcgc cctgcccgac gcgtatttgt cggtggtcga
      781 gtcgctctat cacgccggcg ccttcaacgg gtccgatatc cgcatcgagt ggatctcggc
      841 cgaggaggcc gaagacctcg tcgccagcgg tgagatgggc aatctcgacg gcatcgtgat
      901 ccccggcggg ttcggcgaac gcggcgtcga gggcaagatc gcggccgcgc gctacacccg
      961 cgaacacgac atcccgaccc tcggcctgtg cctgggactg caggtgatga cgatcgagta
     1021 cgcccgcgac gcgttgggca tggacggtgc gaactcggcc gaactcgacc ccgacacccc
     1081 gtatccggtg atcgacatca tggacgacca ggtcgacgtc gaaaacaagg gtggcacgat
     1141 gcgcctcggc gcctatccgg cggtgctcgg tcccggcacc cgggtggccg agatctacgg
     1201 ctcgaccgag gtgagcgagc ggcaccgaca ccgctacgag ttcaacaacc ggttccgcga
     1261 gcgcttcgag ggcaccgact tcgtcatctc gggcacctcg cccgacggtc gtctggtcga
     1321 gttcatcgag ttggcgaatc acccgttctg gatcggcacc caggcgcacc cggaactcaa
     1381 gagccggccc acccacccgg cgccgctgtt tcgggaattc gtggtcgcgg cccgggcccg
     1441 ggcctgtgat gaactcaggc tgcgggtgcg tgacga
//
LOCUS       DAOOMA010000650         1467 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_114389_length_1467_cov_2.004249, whole genome shotgun
            sequence.
ACCESSION   DAOOMA010000650 DAOOMA010000000
VERSION     DAOOMA010000650.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 1467)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1467)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1467
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_114389_length_1467_cov_2.004249"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1109)
                     /locus_tag="PLP95_13530"
     CDS             complement(<1..1109)
                     /locus_tag="PLP95_13530"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF013180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HPB46871.1"
                     /translation="MAAGALGLDDLVTAMPHAAAVLNRQGIAVVANPAWVGVAADLAG
                     IFPQAVEEVVDLEPVIDLVDRRRSPADAIRAAIRGGIPEVVERLRLRSTTARSAEPRV
                     VEVRVAAIGVASEYVLATCIDVTSGAGDGGWDLVDRHPLTGLPGRRLFLELLGQAMTM
                     SDRHRYHPAALAVSVTGLDDVAGSHGPEARDELTVAVASRLKAGLRPGDVVAQTGDDL
                     FVIMLPDVDGAYLAEAVAYRLGVEVGRPFRLGQHRAEVQLFVGLATAESSKSPELVLA
                     SAEAALAAALVHPAEEVVATRRVVVHGAGGDGHLSEAGGAARRIDVAELREEELVSFF
                     QPILDITDRRVVAGEALMRWRHPHYGVLSAGEFLGL"
     gene            complement(1251..>1467)
                     /locus_tag="PLP95_13535"
     CDS             complement(1251..>1467)
                     /locus_tag="PLP95_13535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020378381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HPB46872.1"
                     /translation="SGDVLVRDGRVPADLAVGDILCTPVTGAYGHSMGSNYNKVLRPP
                     IVFVRSGAARLVVRRETNEDLIRLDVG"
BASE COUNT          260 a          522 c          495 g          190 t
ORIGIN      
        1 agccccagga actcgccggc agacagcacg ccgtagtggg ggtgacgcca gcgcatcagc
       61 gcttcgccgg cgacgacccg gcgatcggtg atgtcgagga tcggctggaa gaagctcacc
      121 agctcctcct cgcgcaactc ggcgacgtcg attcgacgcg ccgccccacc ggcctcgctc
      181 agatgaccgt cgccgcccgc gccgtgcacg acgactcgac gtgtcgcaac gacctcctcg
      241 gcggggtgaa ccagtgccgc ggccaacgcc gcctccgcgc tcgcgaggac gagttcaggg
      301 ctcttcgacg actcggccgt cgcgaggccg acgaagagct gtacctcggc acgatgctgg
      361 ccgagccgga acgggcgacc cacctcgacg ccgaggcggt aggcgacggc ctcggcgagg
      421 taggcgccgt cgacatcagg cagcatgatg acgaacaggt cgtcaccggt ctgcgccacc
      481 acgtcgcctg gtcgcaatcc agccttaagg cgcgacgcga cggcgacggt caactcgtcg
      541 cgggcttcag ggccgtggga tccggcgacg tcgtcgaggc cggtgacgct gaccgccagc
      601 gcggcggggt gatagcgatg gcggtccgac atggtcatcg cctgaccgag tagctcgagg
      661 aacaggcgac ggcccggcag gccggtgaga ggatgtcgat cgacgagatc ccacccaccg
      721 tccccggcgc cgctggtgac gtcgatgcat gtagcgagca cgtactccga ggccacaccg
      781 atggcggcga cgcgcacctc gacgacgcgg ggttcggccg atcgggcggt ggtcgagcga
      841 aggcgaagcc gttcgacaac ctcgggaatc ccgccacgga tcgcggcgcg gatcgcgtcg
      901 gccggcgacc gacgccgatc gaccagatcg atcaccggtt cgaggtcgac gacctcctcg
      961 accgcctgag ggaagatgcc ggcgaggtcg gctgccactc ccacccacgc cggattcgcc
     1021 acaacagcga tcccctgccg gttcaacacc gcggcagcgt gcggcatcgc ggtcaccagg
     1081 tcatccaatc ccaaggcacc tgctgccacg tcatcctcca ccatgtcggg accgctcgtt
     1141 gcggagcgct gcgatacacc cagcaccgac cggcacgttc ggtcgacgtg tcgagactac
     1201 gcaccaccgc cgcggaatcg gcggatgggg ccccttcgga agggggccga tcagccgacg
     1261 tcgaggcgga tgaggtcctc gttggtctcg cgccggacga ccagccgagc ggcgccgctg
     1321 cggacgaaca cgatcggcgg acgcagcacc ttgttgtagt tcgatcccat cgaatgcccg
     1381 tacgcgccgg tcaccggagt acacaggatg tcgcccacgg cgagatccgc gggaacccgg
     1441 ccgtcgcgaa ccaaaacgtc acccgac
//
LOCUS       DAOOMA010000651         1457 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_115533_length_1457_cov_4.029244, whole genome shotgun
            sequence.
ACCESSION   DAOOMA010000651 DAOOMA010000000
VERSION     DAOOMA010000651.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 1457)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1457)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1457
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_115533_length_1457_cov_4.029244"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..348
                     /gene="bfr"
                     /locus_tag="PLP95_13540"
     CDS             <1..348
                     /gene="bfr"
                     /locus_tag="PLP95_13540"
                     /EC_number="1.16.3.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004322 - ferroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_process: GO:0006880 - intracellular sequestering of
                     iron ion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin"
                     /protein_id="HPB46873.1"
                     /translation="DESIDEMRHAEELIDRVLYLDGVPNLQRLYALRVGEDVVEQFNN
                     DLAVEREAVDRLRRGIALCNEVADHGTRQLFEKILVAEEEHIDWLETQLTTIEQIGVQ
                     NYLSEQLGEHPSH"
     gene            complement(382..1329)
                     /locus_tag="PLP95_13545"
     CDS             complement(382..1329)
                     /locus_tag="PLP95_13545"
                     /inference="COORDINATES: protein motif:HMM:TIGR03621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03621 family F420-dependent LLM class
                     oxidoreductase"
                     /protein_id="HPB46874.1"
                     /translation="MAHPRRFRFGAELHRPFPDMTWAETAREVESLGYSTLFVPDHFQ
                     DQFAPIAALASAATATSDLKVGTLVLDNDYRHPVITAMEMATIDAISEGRLELGLGAG
                     WKRLDYDQGGIAYDEPRVRVERFMEAVEVVRALFGDDPVNFSGDYYTITDMVGAPRPH
                     TVGGPPLLLAGGARRMLRFAGQHAAIVGVNPSIHSGEVDAAAARDALSDRIDEKFSWV
                     REGAGERFDDIEFNAWCSVATITDDPLGFAEMVAPGFGIAAEDAHTALDSPITMIGTL
                     ASLGEQLHRRRERWGYSYHVFADDAVRPMAPLVAAYRDA"
BASE COUNT          263 a          486 c          481 g          227 t
ORIGIN      
        1 gacgagtcca tcgacgagat gcgccacgcc gaggaactca tcgaccgggt cctgtatctc
       61 gacggtgtgc cgaaccttca gcggctgtat gcgttgcgcg tcggcgagga cgtcgtcgag
      121 cagttcaaca acgatctggc cgtcgagcgc gaggcggtcg accgcctgcg acgcggcatc
      181 gcgttgtgca acgaggtcgc cgaccacggc acgcgccaac tcttcgaaaa gatcctcgtc
      241 gccgaggaag aacacatcga ctggctcgaa acccagctca ccacgatcga acagatcggc
      301 gtgcagaact acctgagcga gcagctcggc gagcacccct cgcactgacc agcgagatgg
      361 tggctcaggc gagatggcgg ctcaggcgtc gcggtaggcg gcgacgagtg gagccatcgg
      421 ccgcacggcg tcgtcggcga acacgtggta gctgtagccc cagcgctcgc gtcgacggtg
      481 cagttgctcg ccgagactcg ccagcgtgcc gatcatcgtg atgggcgagt cgagcgcggt
      541 gtgcgcatcc tctgcagcga tgccgaagcc cggcgccacc atctctgcga acccgagcgg
      601 atcgtcggtg atggtcgcga cgctgcacca ggcgttgaac tcgatgtcgt cgaagcgctc
      661 ccccgccccc tcgcgcaccc acgagaactt ctcatcgatg cgatccgaga gcgcgtcgcg
      721 agccgctgcc gcatcgacct cacccgagtg gatcgacgga ttcaccccca cgatcgccgc
      781 gtgctgccca gcgaagcgca acatgcgccg ggcgccgccc gcgagcaaca acggaggccc
      841 accgaccgtg tgaggccgcg gcgcacccac catgtcggtg atcgtgtagt agtcgcccga
      901 gaagttgacg gggtcgtcgc cgaacagcgc ccgaacgacc tcgaccgcct ccatgaaccg
      961 ctcgacgcga acgcggggtt cgtcatacgc gatgccgccc tggtcataat cgaggcgctt
     1021 ccaaccggcg ccgaggccga gttcgagccg accctcgctg atcgcgtcga tcgtcgccat
     1081 ctccatcgcg gtgatgaccg gatggcggta gtcgttgtcc aacacgaggg tgccgacctt
     1141 caggtcgctg gtagccgtgg ccgccgacgc cagcgcggcg atcggtgcga actggtcctg
     1201 aaagtgatcg ggaacgaaca gcgtcgagta tccgagcgac tccacctcgc gggcggtctc
     1261 agcccaggtc atgtcgggaa acggtcggtg cagttcggct ccgaatcgga aacgtcgtgg
     1321 gtgggccatg ggggttcctc cttagcgatg gtccgccgga cgggtcgagt gtgtcagtcg
     1381 cgaagcgacg gttgagcacc atccggcctc aacggcaccg gtccctgcga cgcgttgacc
     1441 agatcggcga tcagttg
//
LOCUS       DAOOMA010000652         1455 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_115874_length_1455_cov_1.665000, whole genome shotgun
            sequence.
ACCESSION   DAOOMA010000652 DAOOMA010000000
VERSION     DAOOMA010000652.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 1455)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1455)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1455
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_115874_length_1455_cov_1.665000"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..859
                     /locus_tag="PLP95_13550"
     CDS             <1..859
                     /locus_tag="PLP95_13550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB46875.1"
                     /translation="SVAEITARVAARFPGVVLPPRPQLDEVLARADLRVEWSDQLQAF
                     ASSESGPGGIGTLTRVTTRRGTAFGTDGTTLAPAPDALDPELAEAIEVDARLSRSLAE
                     GGFLALRVPTDRLAQAQRGLARYENPAGAQPAMTHVDLEATFLAHLRSNAQQRGVAWA
                     NLEGADDPADPNWAKLSILATAAVDATIDEVARHSNVVAWYPGALVRHGSGAGAGAAT
                     APLDRLRDLVFDGEHPLRTLWLVVLGGTTDALPKVDGTPVPTLAASQWMDMNDAWLKN
                     VHRAGGLNP"
     gene            856..>1455
                     /locus_tag="PLP95_13555"
     CDS             856..>1455
                     /locus_tag="PLP95_13555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB46876.1"
                     /translation="MIDSTLLVSTLKGQLNDLLADLGRQVDTVADIGDPLRREYQAAF
                     DAERTGWTFSEWADDRLTQVAVGWLLGCVFVRFCEDNALVDDPMISGRSDGERGNAAV
                     LARAAQQDYFRANPTHSDREYLQHAFRRAALLPGLDGVLGEGHSPLWLTDPSADACTE
                     LLALFRRTDDAGDLVLDFTDPQRSTRFLGDLYQDLSEHAK"
BASE COUNT          207 a          498 c          503 g          247 t
ORIGIN      
        1 gagcgtcgcc gagatcaccg cacgggtcgc ggcccgcttt ccgggggtgg tgttgccgcc
       61 gcggcctcaa ctcgacgagg tgttggcccg tgccgatctg cgcgtcgagt ggtccgatca
      121 gcttcaggcc ttcgcgtcga gcgagtcggg tccgggcggg atcggcacgc tcacccgggt
      181 cacgacccgc aggggcacgg cgtttggcac cgacggcacc accctcgccc cggcgcccga
      241 cgcgctcgac cccgaactcg ccgaggcgat cgaagtggac gcccgcctga gccgctccct
      301 cgccgagggc gggtttttgg cgttgcgggt gcccaccgat cgcctcgctc aggcccaacg
      361 tggtctggcc cggtacgaga accctgccgg cgcccagccg gcgatgaccc atgtcgatct
      421 cgaagcgacc tttctcgctc atctccgctc gaatgcccaa cagcgtggtg tggcgtgggc
      481 gaacctcgaa ggcgcggatg atccggccga tccgaactgg gcgaaactgt cgatcctcgc
      541 caccgccgcg gtcgacgcca cgatcgacga ggtcgcccgc cacagcaatg tcgtggcctg
      601 gtatccgggt gcgttggtgc gtcacggttc cggtgcgggt gcgggcgcag ccacggcccc
      661 gctcgaccgt ttgcgcgacc tcgtgttcga cggcgagcat ccgttgcgca ccctgtggct
      721 ggtggtgttg gggggaacga ccgacgcgtt gcccaaggtg gatggcaccc cggtgccgac
      781 gctggcggcc agccagtgga tggacatgaa cgacgcctgg ttgaagaacg ttcatcgggc
      841 gggaggcctg aacccatgat tgattcgacg ttgttggtct cgaccctgaa gggccaactc
      901 aacgatctgt tggccgacct gggccgccag gtcgacaccg tggccgacat cggcgacccg
      961 ttgcgccgcg aataccaggc ggccttcgac gccgagcgca ccgggtggac cttcagcgag
     1021 tgggccgatg accgtctcac ccaggtggcg gtcggctggc tgctggggtg cgtgttcgtg
     1081 cggttctgcg aggacaacgc gttggtggac gacccgatga tttcgggccg cagcgacggc
     1141 gaacgcggca acgcggcggt gttggcccgc gccgcccagc aggactattt ccgggcgaac
     1201 cccacccatt ccgaccgcga gtatctccag cacgcgtttc gccgtgcggc gctgttgccg
     1261 ggcctcgatg gtgtgttggg tgagggccac agcccgttgt ggctcaccga cccgtcggcc
     1321 gatgcgtgca ccgagttgtt agccctgttt cgccgcaccg acgacgccgg cgatctggtg
     1381 ctcgatttca ccgaccccca gcgcagcacc cgctttctcg gcgacctgta ccaggacctg
     1441 tccgagcacg cgaag
//
LOCUS       DAOOMA010000653         1443 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_117303_length_1443_cov_4.000720, whole genome shotgun
            sequence.
ACCESSION   DAOOMA010000653 DAOOMA010000000
VERSION     DAOOMA010000653.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 1443)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1443)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1443
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_117303_length_1443_cov_4.000720"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..258)
                     /locus_tag="PLP95_13560"
     CDS             complement(<1..258)
                     /locus_tag="PLP95_13560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015798981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB46877.1"
                     /translation="MAVTDIPGFIADLKDHATRHGFHIHDERHFIETYSMRQAWEVDL
                     HPEEACGGPLDLHLALEVEPRAILAFEDLVVGLPEVEARTPS"
     gene            313..1053
                     /locus_tag="PLP95_13565"
     CDS             313..1053
                     /locus_tag="PLP95_13565"
                     /inference="COORDINATES: protein motif:HMM:NF012673.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0046855 - inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HPB46878.1"
                     /translation="MENATTFALFDRIAAEVADAVAVADRVGRGERPGQYRLDLDADA
                     VVTEALRSAGFGVVSEESGRDGYGAAVTVVVDPVDGSTNASRSLPCWATSLCAVDADG
                     PLLATVVDQSRRVTWRAVRGGGATRQVGDGQPEPIRVAEPVLAGDAVVAINGMSSNKA
                     ETWQFRAYGAASIELCMVADGTLDGYTNLDGDSHGVWDYLAAVLILAEAGGVARDVEF
                     RDLVTLNPRERRCIVAASCDSLLAEMMW"
     gene            1053..>1443
                     /locus_tag="PLP95_13570"
     CDS             1053..>1443
                     /locus_tag="PLP95_13570"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HPB46879.1"
                     /translation="MGSSRTQRWSLQVYGRLPSMMRQRLVRAFKPSYTVGSMTAVTRS
                     DGAVLHVRHSYASHWALPGGLVNRREDVATGAVRETREEVGIEVVLHGQPSVTVDPIK
                     QVVRVIYRAGLAEGVVATDAHPASAEIV"
BASE COUNT          223 a          438 c          538 g          244 t
ORIGIN      
        1 ggacggcgtc cgcgcctcca cctccggaag cccgacgacg aggtcttcga acgcgaggat
       61 ggcccgcggc tcaacctcaa gcgcgaggtg cagatcgagc gggccgccgc atgcctcctc
      121 gggatgaaga tcgacctccc acgcctgacg catggagtag gtctcgatga agtgccgctc
      181 gtcgtggatg tggaagccgt gacgggttgc atggtctttg agatccgcga tgaagcctgg
      241 gatgtcagtc actgccattg ggagccagtc tcgcaggtcc gtcaaggcac tgcgacccga
      301 taccgtggcg gtgtggagaa tgccacaacg ttcgcgttgt tcgacaggat cgcagccgag
      361 gtcgccgacg cggttgccgt ggcggatcgc gtcggtcggg gcgagcggcc aggacagtac
      421 cgcctcgacc tcgatgccga cgcggtggtc accgaggcac tgcgctcggc agggttcggt
      481 gtggtgtcgg aggaatccgg acgcgacggc tacggtgcgg cggtgaccgt cgtggtcgac
      541 cccgtcgacg gctcgacgaa cgcgagccgc tcgctgccgt gctgggcgac gagtctgtgc
      601 gccgtcgatg cggacggccc gctgttggcg accgtcgtcg accagagccg tagggtcacc
      661 tggcgtgcgg tgcgaggcgg cggagcgacc cgccaggtcg gtgacggtca gcccgagccg
      721 attcgggtgg cggaaccggt gctggccggc gacgccgtcg tcgctatcaa cggcatgtcg
      781 tccaacaagg cggaaacctg gcagttccgg gcgtacggag cggcctcgat cgagttgtgc
      841 atggtcgccg atggcaccct cgacgggtac acgaacctcg acggagactc ccatggggtg
      901 tgggactacc tcgcggccgt attgatcctc gcggaggccg gaggggttgc ccgcgacgtc
      961 gagttccgcg acctggtcac gctgaatcct cgtgaacggc gctgtatcgt cgcggcgtcc
     1021 tgcgactcgc tgttggcaga aatgatgtgg tgatgggttc aagccggaca caacgttgga
     1081 gccttcaggt gtacgggcgg ttgccgtcga tgatgcgcca gcgcctcgtg cgggcgttca
     1141 agccctccta cacggtgggg tcgatgacgg cggtgacccg ctccgatggg gcggtgttgc
     1201 acgtgcggca cagctacgcg tcgcactggg cgttgcccgg gggactggtg aatcgccgtg
     1261 aggacgtcgc gacgggcgcg gttcgagaaa cccgtgagga ggtcggcatc gaggtggtgt
     1321 tgcacggcca accgtcggtc accgtcgatc cgatcaaaca ggtggtgcgg gtcatctacc
     1381 gggccggcct cgccgagggg gtcgtggcga ccgacgccca tcccgcatcg gcggagatcg
     1441 tcg
//
LOCUS       DAOOMA010000654         1441 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_117580_length_1441_cov_3.310245, whole genome shotgun
            sequence.
ACCESSION   DAOOMA010000654 DAOOMA010000000
VERSION     DAOOMA010000654.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 1441)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1441)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1441
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_117580_length_1441_cov_3.310245"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(18..1091)
                     /gene="gcvT"
                     /locus_tag="PLP95_13575"
     CDS             complement(18..1091)
                     /gene="gcvT"
                     /locus_tag="PLP95_13575"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HPB46880.1"
                     /translation="MTIQHSPLDATHRELGAKMVPFGGWDMPLSYPAGTLAEHQACRS
                     AAVVFDVSHLGTVRVSGPGAFDALQRAFTNDLAKIEPGRCQYTHSLSEDGSVADDIIV
                     WWRAADVFDVMPNASNTDRICRALVDGAAGAEVKVDDVTTERAVIAVQGPQARELLAP
                     LLPEAAAVGRFRIIETTFDGTPIVIAGTGYTGEDGLEIAVPAQRAAELWRAVTAMGIE
                     PAGLGARDTLRLEAGLPLHGHELGDGITSLNADLEWVVSWTKGDFVGREALAAQKEAG
                     VTPILRGLSVDTKRPPRAEMDVYRGDTLVGRITSGNISPVLGHGIAMAMIEPGLELGS
                     VLEVDLRGKRVPATVVEMPFVKR"
     gene            complement(1114..>1441)
                     /locus_tag="PLP95_13580"
     CDS             complement(1114..>1441)
                     /locus_tag="PLP95_13580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012228598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HPB46881.1"
                     /translation="LELKVIKSLLDAGIKLEQVRQLFAYLTEHLGEDVTTANLVISGS
                     GTVLVRTGEELIDALQSGQGVLNVLPLGGVKDELDATIVELYPSRPAAAGPSDGVADG
                     PAALAQ"
BASE COUNT          218 a          495 c          488 g          240 t
ORIGIN      
        1 tgggcggtgg gttcggttca gcgcttcacg aacggcatct cgacgacggt cgccggcacc
       61 cgctttccgc ggaggtcgac ctcgagcacc gagccgagtt cgaggcccgg ctcgatcatg
      121 gccatggcga tgccgtggcc aagaacgggt gagatattgc ccgaggtgat ccgccccacc
      181 agcgtgtcgc cccggtagac gtccatctcg gcacgcggcg ggcgtttcgt gtcgaccgac
      241 aacccgcgca ggatgggggt gacccccgcc tccttttgcg ccgcgagcgc ctcgcgaccc
      301 acgaagtcgc ccttcgtcca cgacacgacc cactcgagat cggcgttgag gctggtgatg
      361 ccgtcgccga gttcgtggcc gtgcagcggg aggccggcct cgaggcgcag cgtgtcgcgg
      421 gccccgagtc cggccggctc gatccccatc gccgtcacgg cgcgccacag ctctgctgcc
      481 cgttgcgccg gaaccgcgat ctcgaggccg tcctcgccgg tgtatccggt gcccgcgatg
      541 acgatcggcg tgccgtcgaa ggttgtctcg atgattcgga atctccccac cgcggccgcc
      601 tcgggcaaca gcggggcgag caattcgcgg gcctggggcc cttggacggc gatcaccgca
      661 cgttcggtgg tgacgtcgtc gaccttgacc tcggcgcccg ccgctccgtc gacgagcgcg
      721 cggcaaatcc gatcggtgtt cgatgcgttc ggcatcacgt cgaacacgtc ggccgcacgc
      781 caccacacga tgatgtcgtc ggccaccgaa ccatcctcgg acagcgaatg ggtgtactgg
      841 cagcgaccgg gttcgatctt ggcgaggtcg ttggtgaagg cccgctgcaa cgcgtcgaac
      901 gcccccgggc cactgacgcg caccgtgccg aggtggctca catcgaacac gaccgccgcg
      961 ctgcggcaag cctggtgctc ggcgagggtg cccgccggat acgaaagcgg catgtcccat
     1021 ccgccaaagg gcaccatttt ggcgccgagt tcacggtggg ttgcgtcgag cggtgagtgc
     1081 tgaatcgtca ccgccgcagt ctagaggggc gcgttattgc gccagagccg ccggcccgtc
     1141 cgccacgcca tccgaaggcc cggcggccgc cggccgggac gggtacagct cgacgatcgt
     1201 cgcgtcgagt tcgtccttca ccccgccgag cggcaacacg ttcagcaccc cttgtccgga
     1261 ctggagcgcg tcgatgagtt cctcgccggt acgaaccagc acggttcccg aaccgctgat
     1321 gaccaggttg gcggtggtga cgtcctcgcc gaggtgttcg gtgagatagg cgaacagctg
     1381 gcggacctgt tcgagtttga tgccggcatc gagcaggctc ttgatcacct tgagttcgag
     1441 c
//
LOCUS       DAOOMA010000655         1426 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_119599_length_1426_cov_2.975930, whole genome shotgun
            sequence.
ACCESSION   DAOOMA010000655 DAOOMA010000000
VERSION     DAOOMA010000655.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 1426)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1426)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1426
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_119599_length_1426_cov_2.975930"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..615)
                     /locus_tag="PLP95_13585"
     CDS             complement(<1..615)
                     /locus_tag="PLP95_13585"
                     /inference="COORDINATES: protein motif:HMM:NF016196.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF445 family protein"
                     /protein_id="HPB46882.1"
                     /translation="MAASFHLTSDDRQRLADLRRMRRLASLVLLGATIVFVIARLLEV
                     RVDDTWGYLRATAEAGMVGGIADWFAVTALFRHPLGIPIPHTAILPNRKDQLGRTFGG
                     FVQGSFLAPEVLTQRLEGVSVSDRMAQWLLESDNSELAAEKAAALLATIARLLPDEEI
                     GDLLVNDVLGRLDQVDVAPIAGQVLDIATEDGRHHQLLDAVLKGL"
     gene            631..>1426
                     /locus_tag="PLP95_13590"
     CDS             631..>1426
                     /locus_tag="PLP95_13590"
                     /inference="COORDINATES: protein motif:HMM:TIGR03317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB46883.1"
                     /translation="MGNLVGAWVERSVITVEGPDSASYLQGQLSQDVASMNVADSRWS
                     LLLAPSGKTVAWLRVHRVGDEAFVLDLEPAAAPDALARLERFKLRVEVAMSLAVGHQM
                     LSVRSLADADAGAGADADVVVPAQAASVPVGAPAGSSVGVRGVDLICSDPALIAAPAG
                     MELDPVAVERLRISHGVPTFGVDFDEDTIPAELGPWLVEESVSWTKGCYTGQELVARV
                     DARGNNTPRHLSVLVFDDPGAMVQIGAEVVANTLSDGVVGAVTSPYH"
BASE COUNT          230 a          434 c          516 g          246 t
ORIGIN      
        1 gagccccttg agcaccgcgt cgagcagctg gtgatggcgg ccgtcctcgg tggcgatgtc
       61 gaggacctgc ccggcgatcg gtgcaacatc gacctgatcg aggcggccga ggacgtcgtt
      121 gacgagcagg tcgccgatct cctcgtccgg caacagacgg gcgatcgtcg ccaacagcgc
      181 cgccgccttc tctgccgcga gttcggagtt gtcggactcg agcagccatt gcgccatgcg
      241 gtccgacacg ctgacccctt cgaggcgttg agtgagtacc tcaggagcga ggaagctgcc
      301 ctggacgaac ccgccgaagg tgcgtccgag ctgatccttt cggttgggga ggatggcggt
      361 gtgtgggatc ggaatcccca acgggtggcg aaacagcgcg gtgacggcga accagtcggc
      421 gatccccccg accatcccgg cctcggcggt tgcccgcaaa tatccccagg tgtcgtcgac
      481 gcggacctcg aggaggcgag cgatgacgaa cacgatggtc gcgcccagca gcaccagcga
      541 tgcgagccga cgcatacggc gcagatcggc gaggcgttgt cgatcgtccg atgtcagatg
      601 aaagctggca gccatggaag actcaaccct atgggcaacc tcgttggggc atgggtcgaa
      661 cgcagtgtga tcacggtcga aggaccggac tcggcgtcgt atctgcaggg acaactgagc
      721 caggacgtcg cctcgatgaa cgttgccgac agtcggtggt cgttgctgct cgctccgagc
      781 ggaaagaccg tggcgtggct gcgggtgcat cgtgtcggcg acgaggcgtt cgtgctcgac
      841 ctcgaaccgg ccgcggcccc ggacgcgctc gctcggctcg aacggttcaa gttgcgagtc
      901 gaggtggcga tgtcgttggc ggtgggccac cagatgttgt cggtgcggtc gctcgccgat
      961 gccgatgccg gtgccggtgc cgatgccgat gtcgtggtgc cggcacaggc ggcgagtgtg
     1021 ccggtcgggg cgccggccgg gtcgtcggtg ggggtgcgcg gagtcgacct catctgttcc
     1081 gacccggcgc tgatagcggc ccccgcggga atggaactcg acccggttgc ggtcgaacgc
     1141 ctgcgtatct cacacggagt tcccaccttc ggtgtcgact tcgacgagga cacgattccc
     1201 gcggagctgg gcccgtggct cgtggaggaa tcggtgagtt ggaccaaggg gtgttacacg
     1261 gggcaggaac tcgtcgcccg ggtcgatgcc cggggcaaca acaccccgcg gcatctgagc
     1321 gtgctcgtct tcgacgaccc cggtgccatg gtccagatcg gtgcggaggt cgtggcgaat
     1381 accctttccg atggtgtggt gggcgcggta acctcgccgt atcatt
//
LOCUS       DAOOMA010000656         1417 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_120779_length_1417_cov_3.333333, whole genome shotgun
            sequence.
ACCESSION   DAOOMA010000656 DAOOMA010000000
VERSION     DAOOMA010000656.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 1417)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1417)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1417
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_120779_length_1417_cov_3.333333"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..702
                     /locus_tag="PLP95_13595"
     CDS             <1..702
                     /locus_tag="PLP95_13595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020378645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HPB46884.1"
                     /translation="ARSFSFQPGPVFCSLLLADEINRATPKTQSALLEAMQERRVTAG
                     GETRPLPRPFLVLATQNPIEQEGTYPLPEAQLDRFLLKAEVGMPTAAELTQIIERTTG
                     SHQATPRPVTTPGEVSEMIALTRSVPVPEAVVRFAATIVVATHPNSEGAPDAVRRYVE
                     AGSSPRGAQALILAAKAVALMDGRPSVSSDDIRSVAQPALRHRVALGYDAINDDVAVS
                     SVIDAVVESAATVAL"
     gene            745..>1417
                     /locus_tag="PLP95_13600"
     CDS             745..>1417
                     /locus_tag="PLP95_13600"
                     /inference="COORDINATES: protein motif:HMM:NF013998.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HPB46885.1"
                     /translation="MLERDERRRLERLAVRLDRRVGAGFSGSHRSSQRGRSLDFADWR
                     DYLPGDDVRSIDVQAWARLDQVVIRLFEADVDLSAQLLLDTSGSMAGVAGDRSDTGSI
                     GRTKFFQAQRVAAAIGIILLTRNESISLTTFDDRVAARYHSRTAAAKFLAHLERLSAA
                     GPTPLADRANAIVTRRPRRGLMVVISDFLTDEWELALGRLAARGEPVLAICITDPADD
                     APDLAG"
BASE COUNT          218 a          508 c          469 g          222 t
ORIGIN      
        1 gcacgcagct tctcgttcca gcctgggccc gtgttctgca gcctgctgct ggccgacgag
       61 atcaaccgcg ccacgcccaa gacccagtcg gccctgctcg aggcgatgca ggagcggcgg
      121 gtgaccgccg gcggcgagac ccgaccgctg ccgcgcccgt tcctggtgct ggcgacgcag
      181 aacccgatcg aacaggaagg cacctacccg cttcccgaag cccagctcga ccggttcctg
      241 ttgaaggccg aggtcggcat gccgaccgca gccgaactca cccagatcat cgagcgcacg
      301 accggctccc atcaggccac accccgcccc gtgaccaccc cgggtgaggt ctccgagatg
      361 atcgcgttga cgcgttcggt tccggtgcct gaggcggtgg ttcgcttcgc cgccaccatc
      421 gtggtggcca cccatcccaa cagcgagggt gcccccgatg ccgtacgccg ttacgtcgag
      481 gccgggtcga gcccccgcgg cgcacaggcc ttgatcctcg cggcgaaggc ggtcgcgctg
      541 atggacggcc ggccgtcggt gagctcggat gacattcgat cggtcgcgca gcccgcgctt
      601 cgccaccgcg tcgccctggg ctacgacgcg atcaacgacg acgtcgccgt gtcgagcgtg
      661 atcgatgcgg tggtcgaatc ggccgccacg gtggcgttgt aggcgtcgcg gtcgggatcg
      721 cgaacggcga gagcagggtt cgacgtgttg gaacgagacg agcgcaggcg gctcgaacgt
      781 ttggcggtgc ggctcgatcg gcgcgtcgga gcagggtttt ccggctcaca ccggtccagc
      841 caacgcgggc gatccctcga cttcgccgat tggcgcgact acctccccgg cgacgacgtc
      901 cgctccatcg acgttcaggc atgggcacgc ctcgaccagg ttgtcatccg cctgttcgaa
      961 gccgacgtcg acctgtcggc ccagttgctg ttggatacct ccgggtcgat ggccggcgtc
     1021 gctggcgacc ggtcggatac cggcagcatt gggcgaacaa agttcttcca ggcgcaacgg
     1081 gtcgcggcgg cgatcggcat catcttgttg acccgcaacg agtcgatatc gctgaccacc
     1141 ttcgacgacc gcgtcgccgc ccgctaccac tcgcgaacgg cggccgcgaa gtttctcgcc
     1201 cacctcgaac ggctcagcgc agccgggccg acgccactcg ccgacagagc aaacgccatc
     1261 gtgacccgac gcccgcgccg cggactcatg gtggtgatct ccgacttcct gaccgacgag
     1321 tgggaactcg cgctcgggcg gttggcggcg cgtggcgaac cggtgttggc catctgcatc
     1381 accgaccccg ccgacgacgc ccccgatctg gccggtg
//
LOCUS       DAOOMA010000657         1416 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_120922_length_1416_cov_3.271124, whole genome shotgun
            sequence.
ACCESSION   DAOOMA010000657 DAOOMA010000000
VERSION     DAOOMA010000657.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 1416)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1416)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1416
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_120922_length_1416_cov_3.271124"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..723)
                     /locus_tag="PLP95_13605"
     CDS             complement(<1..723)
                     /locus_tag="PLP95_13605"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HPB46886.1"
                     /translation="MTITEFDGKVAVITGGAAGIGRAMARRFGSAGMKLVLADIETAV
                     LDATVAEFQAEGIDTTGVVADVSKRQDIERIAAVAMEAHGGIHLACNNAGVSGGGLSW
                     EVSEADWKWILEVDLWSVIHGVGVFTPLIEQSGGGHIVNTASMAGVTSPPFMSPYNVA
                     KHGVVALSESMFHELAFLQSSVGVSVLCPGWVRTQIAHSERNRPTEGAHAAAGLEPED
                     LGEDGPAAIFRSLVDDGIDPAAV"
     gene            complement(878..>1416)
                     /locus_tag="PLP95_13610"
     CDS             complement(878..>1416)
                     /locus_tag="PLP95_13610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HPB46887.1"
                     /translation="GYQVLTLEDVVDTADIFITTTGNKGIISAEHMARMKHQAIVGNI
                     GHFDNEIDMAGLAKMSDVRRINIKPGTDEFVFPDGHSVIMLAEGRLLNLGCATGHPSF
                     VMSNSFTNQVIAQIELFTKTELYPVGVYVLPKHLDEKVARLHLDSLGVKLTKLTDEQA
                     DYLGVPVEGPYKPDHYRY"
BASE COUNT          256 a          467 c          437 g          256 t
ORIGIN      
        1 gaccgcagcc ggatcgatac cgtcgtcgac caacgagcgg aagatcgcgg ccgggccgtc
       61 ctcgccgagg tcctccggtt cgagcccagc ggcagcatgg gcgccctcgg tcgggcggtt
      121 gcgttcggaa tgcgcgatct gggtccgtac ccaccccggg cacaacaccg agaccccgac
      181 gctcgactgc agaaaggcga gctcgtggaa catggactcg ctgagtgcga ccacgccgtg
      241 cttggcgacg ttgtagggag acatgaacgg cggcgacgtc acgccggcca tcgacgccgt
      301 gttgacgata tggccgccgc cagactgctc gatgagcggg gtgaacacgc cgacaccgtg
      361 aatgacgctc cacaggtcga cctcgaggat ccacttccaa tccgcctcgc tcacctccca
      421 gctgagtccc ccgcccgaaa cgccggcgtt gttgcaggcg aggtggattc ccccatgggc
      481 ctccatcgcc accgctgcga tgcgttcaat gtcctgccgc ttcgacacgt cggcgacaac
      541 gcccgtggtg tcgattccct cggcctgaaa ctcggcgacc gtcgcatcca acacggcggt
      601 ttcgatatct gccaacacga gtttcatccc cgccgaaccg aagcgccggg ccatcgcccg
      661 gccgatgccg gctgcgccgc cggtgatgac tgcgaccttg ccgtcgaact ctgtgatcgt
      721 catggccaga tcatggtgca tccaccgccc gagcgctccc caaacgcccc aaaagcctcc
      781 ccaaacgccc caaaacgccg ttctccgata accagtggtc gtgatcacga ccactggtta
      841 tcggagaacg ggctggcgct ggtcagccgg gagtgactca gtaccggtag tgatccggct
      901 tgtaggggcc ctcgaccgga acgccgaggt agtcggcctg ctcgtcggtc agcttcgtga
      961 gcttcacgcc gaggctgtcg aggtgcaacc gagcgacctt ttcgtcgagg tgcttgggca
     1021 acacgtacac gccgaccgga tacagctcgg tcttggtgaa cagctcgatc tgggcgatga
     1081 cctgattcgt gaacgagttc gacatcacaa aactcgggtg gcccgtcgca caaccgaggt
     1141 tcaacagacg cccctcggcg agcatgatca cggagtgtcc gtcggggaac acgaactcgt
     1201 cggtgcccgg cttgatgttg atgcggcgca cgtcgctcat cttggcaagg ccggccatgt
     1261 cgatctcgtt gtcgaagtga ccgatgttgc cgacgatcgc ctgatgcttc atccgcgcca
     1321 tgtgctctgc ggagatgatg cccttgttgc cggtggtggt gatgaagatg tcggcggtgt
     1381 cgacaacgtc ttcgagcgtg aggacctgat agccct
//
LOCUS       DAOOMA010000658         1390 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_124552_length_1390_cov_4.274157, whole genome shotgun
            sequence.
ACCESSION   DAOOMA010000658 DAOOMA010000000
VERSION     DAOOMA010000658.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 1390)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1390)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1390
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_124552_length_1390_cov_4.274157"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..517
                     /locus_tag="PLP95_13615"
     CDS             <1..517
                     /locus_tag="PLP95_13615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB46888.1"
                     /translation="KNDNVMMTNAVVCVSDRKRAREIAMSLGRGYLNTMVNMYHDTMP
                     PQDNAVVWPNPPHRIPDESTLDYLIEAGYLLCGTPEEVNEQIAKYQEVGCDQLVFGIP
                     TEGFRDEEILEMIELFGTEVIPNYDTDPEHSTTRYRREAKRKYPDFEKPVDPNLKVSV
                     LPESALIQLPE"
     gene            583..1170
                     /locus_tag="PLP95_13620"
     CDS             583..1170
                     /locus_tag="PLP95_13620"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HPB46889.1"
                     /translation="MTSTRDRLLDVAEQRFARSGIAAVTTREIVEAAGQRNTSAVSYH
                     FGSREGLLAAILRRRGGPIDIERGRRRASLGDASHRDLIACLVVPYSAMLDDGGGRAY
                     LRIVDQLRGRFTAWRIESDVESTAQLAAILDEIERGIDASPALRRERIVALIMLMTSS
                     IADRARRLDDGEVPELDHDDYLANLVDMCAAVLQA"
BASE COUNT          249 a          434 c          455 g          252 t
ORIGIN      
        1 caagaacgac aacgtgatga tgaccaacgc cgtggtgtgc gtatcggatc gcaagcgggc
       61 acgtgaaatc gccatgtcgc tcggccgcgg ctatctcaac acgatggtga acatgtacca
      121 cgacacgatg ccgccccagg acaacgccgt cgtgtggccg aaccctccgc atcgcatccc
      181 cgacgagtcg accctcgact acctcatcga ggccgggtac ctgttgtgtg gcacccccga
      241 ggaggtcaac gaacagatcg ccaagtacca ggaggtcggc tgcgatcaac tggtgttcgg
      301 cattccgacc gagggattcc gcgacgagga aatcctcgaa atgatcgaac tgttcggcac
      361 cgaggtgatt ccgaattacg acaccgaccc ggagcactcg accacccggt atcgccgcga
      421 ggccaagcgt aagtacccgg atttcgagaa gccggtcgat ccgaacctga aggtcagcgt
      481 gcttcccgag agcgcgttga tccaactgcc ggagtgaccc gggcgtcggt cgcgtcggcg
      541 tcggcagggg cgccgacgtc gactccggcg aggtcggctc cggtgacgtc gacgcgggat
      601 cggctgctgg atgtcgccga gcagcgcttt gctcgttcgg gcatcgccgc ggtgacgact
      661 cgcgagatcg tcgaggccgc cgggcaacgc aacaccagcg cggtgagcta tcacttcggc
      721 tcccgcgagg ggttgctggc ggcgatcctg cgtcgacgag ggggtccgat cgatatcgag
      781 cggggtcgcc gtcgagcctc gcttggcgac gcgtcgcatc gtgacctcat cgcatgtctc
      841 gtcgtgccgt actcggccat gttggacgat ggtggcggcc gcgcgtactt acgcatcgtc
      901 gatcagttgc gcgggcgctt caccgcatgg cgcatcgaat ccgacgtgga gagcactgct
      961 cagttggcgg ccattctcga cgagatcgag cgcggtatcg acgcgtcgcc ggcgcttcgg
     1021 cgtgaacgca tcgtggccct catcatgttg atgacctcct cgatcgccga tcgggcccgc
     1081 cgtctcgacg acggcgaggt gcccgagctc gaccacgacg actaccttgc aaacctcgtc
     1141 gacatgtgcg ccgcggttct gcaggcgtag gtgtccgcac gacctcgggc ctatttggcg
     1201 tcagcccaac ccgtccacgc gatggttgaa ctccccattg actgaagctt gagcgtgggg
     1261 cgggaccctt gccgacggaa cggcattggg agtggtcggg cgattgaact caccgactag
     1321 cgtcgaacgt ctacgctagt ggtacgtcgg ttaaatagcg aggtgggtta gtgtcgggtt
     1381 atgccgatcg
//
LOCUS       DAOOMA010000659         1387 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_124965_length_1387_cov_2.897147, whole genome shotgun
            sequence.
ACCESSION   DAOOMA010000659 DAOOMA010000000
VERSION     DAOOMA010000659.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 1387)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1387)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1387
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_124965_length_1387_cov_2.897147"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..271
                     /locus_tag="PLP95_13625"
     CDS             <1..271
                     /locus_tag="PLP95_13625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="steroid 3-ketoacyl-CoA thiolase"
                     /protein_id="HPB46890.1"
                     /translation="AMTMSDIDLTEINEAFASVALAWMKEFDADPATTNPNGGAIALG
                     HPLGATGAFLMTKALNELERTDGTNALITMCCGGGLGTGTIISRL"
     gene            312..860
                     /locus_tag="PLP95_13630"
     CDS             312..860
                     /locus_tag="PLP95_13630"
                     /inference="COORDINATES: protein motif:HMM:NF012774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HPB46891.1"
                     /translation="MTAGSARAKASLIAIVALVAGFAGFAAALAGCTGRDGPVHGVLE
                     ANAVVVRHVDGDTLVARIGPDEEKVRLIGIDTPETVDPRQPVQCFGKEASRHLAELVP
                     EGTSLRLEVDEEPRDRYGRLLVYVYRASDGLFVNLDMVEQGYANTLSIAPNLTHRDRF
                     SEAERTARTEGRGLWSNCPADG"
     gene            complement(941..>1387)
                     /locus_tag="PLP95_13635"
     CDS             complement(941..>1387)
                     /locus_tag="PLP95_13635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB46892.1"
                     /translation="EVSHVSSYLDAMLLRPEFFDIYVDLALEFELPVRIAGPAAEQGA
                     GFRFRQLASEEGLWFPDELIELGSAGIVGRLIEVTDSLEPGVTEIVLRPCVDSPEARA
                     IGAEWDGRVADLAHLSDPGPLAELLESRGVKTIGWRMVRDALRSAG"
BASE COUNT          260 a          501 c          433 g          193 t
ORIGIN      
        1 ggccatgacc atgtccgaca tcgacctcac cgagatcaat gaagcgttcg cttccgtcgc
       61 gctggcgtgg atgaaagaat tcgacgccga cccggcgacc accaacccga acggcggggc
      121 cattgcgctg gggcacccgc tcggagccac gggggcgttc ctcatgacca aggcgctcaa
      181 cgaactcgag cgcacggacg gcaccaatgc cctcatcacc atgtgctgcg gcggtggcct
      241 cggcacggga accatcatct cccggctctg acgaagcgac ggatacgaaa ccgtcgcggt
      301 ccgcgtcgaa cgtgaccgcc ggctcggcga gggcgaaggc ctccctcatc gccatcgtcg
      361 cgctcgtcgc cgggttcgcc gggttcgccg cggcgttggc ggggtgcacc ggtcgcgacg
      421 gacccgttca cggcgtgctc gaggcgaacg ccgtggtggt gcgacacgtc gatggcgaca
      481 ccctcgtcgc ccggatcgga ccggacgagg agaaggttcg cctcatcggc atcgacaccc
      541 ccgagaccgt cgatccacgc cagccggtgc aatgcttcgg caaagaggcc tcgcgtcacc
      601 tcgcagagct cgttccggag ggaacctcgc ttcgcctcga agtcgacgag gagcctcgcg
      661 atcgatacgg ccggcttctg gtgtacgtat atcgagcttc agacggactc ttcgtcaacc
      721 tcgacatggt tgaacagggc tacgcaaaca cgctgagtat cgccccgaac ctgacccatc
      781 gcgaccgttt cagtgaggcc gaacgcacgg cgcgcaccga ggggcgtggg ctctggtcga
      841 actgcccggc cgacggctga ggcgcgggct cggcacatcg accggggcgc cgaccggagc
      901 accgaccgga gcaccgaccg gtgcgccacc ggggcgcagg tcaccctgcc gaacgcagcg
      961 cgtctcgcac cattctccac ccgatggtct tcacgcctcg gctctcgagc agctccgcaa
     1021 gcgggcccgg atccgacagg tgcgccaagt cggcgacgcg accgtcccac tcggccccga
     1081 tcgcgcgcgc ttcaggcgag tcgacgcaag gccgcaacac gatctcggtc acccccggct
     1141 cgagcgagtc cgtcacttca atcaaacgcc cgacgatccc cgcagaaccc aactcgatca
     1201 actcgtcggg aaaccacaac ccctcctcgg acgcgagctg gcgaaaccga aaccccgccc
     1261 cctgctccgc cgctgggccg gcgatgcgga cgggaagctc gaactcgagc gccaggtcga
     1321 cgtagatgtc gaagaactcc ggccgcaaca acatggcgtc gagatacgac gacacatggg
     1381 atacctc
//
LOCUS       DAOOMA010000660         1381 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_125819_length_1381_cov_3.997738, whole genome shotgun
            sequence.
ACCESSION   DAOOMA010000660 DAOOMA010000000
VERSION     DAOOMA010000660.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 1381)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1381)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1381
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_125819_length_1381_cov_3.997738"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..658)
                     /locus_tag="PLP95_13640"
     CDS             complement(<1..658)
                     /locus_tag="PLP95_13640"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HPB46893.1"
                     /translation="MTAQRPRVALTLEQCWHEVPGGTARAALGLIDALRSRDNVAMVG
                     IAARHNSPPAGAYRPSIPVEHVALPRLAMYESWHWLRRPLAESTTGPVDLVHATGIAV
                     PPTKVPLVATLHDLAFLHLPEMYTRRGNAFFRRALELTRRHAAIVCCSSLATLEDCAE
                     AGIDRSRLRHVPLGVDIAPVTDDDRQRVRRRYGLERPFVLWTGTREPRKNLARLLDAF
                     A"
     gene            complement(665..>1381)
                     /locus_tag="PLP95_13645"
     CDS             complement(665..>1381)
                     /locus_tag="PLP95_13645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB46894.1"
                     /translation="KPIRREFFTDGERDRSYYFGDLESWTGYGTDFDDAQRITGAVLA
                     YLGAPRLACAVHHPQIMRRDLWIEAFELWDSLRDDNLICEWAWYFNVSRWRHPERFST
                     NEVYRTLCWPPHLHEFARLYRQPEYQFENFYPEFYEQGRVFEGLPRTVPADDATAARV
                     AVDKLVRWSELGFAVGRLDFANTPDEPWTKGDWKRRVAFRGLWAARKVYDYASLEDRA
                     EANRIHAGDDARDRFTPYEG"
BASE COUNT          216 a          440 c          508 g          217 t
ORIGIN      
        1 gggcgaacgc gtcgaggagt cgtgccaggt tcttgcgcgg ctcgcgggtt ccggtccaca
       61 acacgaatgg ccgctcaagt ccgtaccggc gccggacccg ttggcggtcg tcgtcggtga
      121 ccggagcaat gtcgaccccg agggggacgt ggcgcagtcg tgagcggtcg atgcctgcct
      181 ccgcgcagtc ctcgagggtc gccagcgacg agcagcacac gatggcggcg tgtcgccgcg
      241 tgagttcgag ggcgcgccgg aagaacgcgt tgccccggcg cgtgtacatc tcggggaggt
      301 ggaggaacgc gaggtcgtgc agggtggcga cgaggggcac cttcgtcgga ggcaccgcga
      361 tcccggtggc gtggacgaga tccaccggtc cggtcgtcga ctccgcgagc ggacggcgca
      421 accagtgcca cgactcatac atggcgaggc gggggagcgc gacgtgttcg acagggatcg
      481 acggtcggta ggcaccggcg ggcggcgagt tgtgtcgggc tgcgatgccg accatcgcaa
      541 cgttgtcgcg actgcgcaga gcgtcgatga gtccgagtgc cgcccgggcc gtcccgccgg
      601 gcacctcgtg ccagcactgc tcgagggtca acgcgacgcg cggtcgctgc gccgtcatcg
      661 cggcttaacc ctcatagggg gtgaagcggt cgcgggcgtc gtcgccggcg tgaattcggt
      721 tcgcctcggc gcggtcctcg agcgacgcgt agtcgtacac cttgcgtgcg gcccacaggc
      781 cgcgaaacgc cacgcggcgc ttccagtcgc ccttggtcca cggctcgtca ggcgtgttcg
      841 cgaagtcgag gcggccgacc gcgaatccca gttcgctcca gcggaccagc ttgtcgacgg
      901 cgacccgcgc ggcggtggca tcgtcggcgg gcaccgtgcg cggaagcccc tcgaaaaccc
      961 gcccctgttc gtagaactcg ggatagaagt tctcgaactg gtactcgggc tggcgataca
     1021 ggcgcgcgaa ctcgtgcaga tgcggtggcc agcacagcgt gcggtacacc tcgttcgtcg
     1081 agaaccgctc cgggtgacgc cagcggctga cgttgaagta ccacgcccat tcacagatca
     1141 ggttgtcgtc gcgcaacgag tcccacagtt cgaaagcctc gatccagagg tctcttcgca
     1201 tgatctgcgg gtggtgcacg gcacaagcca ggcggggcgc cccgaggtag gcgagcaccg
     1261 caccggtgat gcgctgggcg tcgtcgaagt cggtgccgta gccggtccag ctctcgaggt
     1321 cgccgaagta gtacgagcgg tcgcgttcgc cgtcggtgaa gaactcgcgt cggatgggct
     1381 t
//
LOCUS       DAOOMA010000661         1379 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_126117_length_1379_cov_3.372356, whole genome shotgun
            sequence.
ACCESSION   DAOOMA010000661 DAOOMA010000000
VERSION     DAOOMA010000661.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 1379)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1379)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1379
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_126117_length_1379_cov_3.372356"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..211)
                     /locus_tag="PLP95_13650"
     CDS             complement(<1..211)
                     /locus_tag="PLP95_13650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB46895.1"
                     /translation="MSSTAIGDDRFGPEPEAHVSDAPTDNDAHESFASSDGSFGVPNV
                     GVPTIDVPTVVVPTVDGPISSVPMIA"
     gene            313..383
                     /locus_tag="PLP95_13655"
     tRNA            313..383
                     /locus_tag="PLP95_13655"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:345..347,aa:Cys,seq:gca)
     gene            497..570
                     /locus_tag="PLP95_13660"
     tRNA            497..570
                     /locus_tag="PLP95_13660"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:531..533,aa:Val,seq:gac)
     gene            complement(770..>1379)
                     /locus_tag="PLP95_13665"
     CDS             complement(770..>1379)
                     /locus_tag="PLP95_13665"
                     /inference="COORDINATES: protein motif:HMM:TIGR01167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009986 - cell surface [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HPB46896.1"
                     /translation="LDPQWSNRAIPTDATWTLEINAPAPLCSPVAATAVIYDMKTTNQ
                     WPQTLSETLDVVFKGAGTYTIEFTKVCRRVQFDLTDGRNPEQLNTGFDHTLLFPGDLG
                     TAHQHLGRAADCEEPTTTTSTTAVAPTSAAPTTTVAVVDSATTIPNDVRAATTVAPGS
                     NGKVGAELAATGSDNVWLAVFGAGLIFLGAALTMGRRRVGDA"
BASE COUNT          231 a          417 c          495 g          236 t
ORIGIN      
        1 cggcgatcat cggcaccgag gagatcggcc cgtcgacggt cggaacaacg acggtcggaa
       61 cgtcaatcgt cggcacccca acgttgggta caccgaagga gccgtccgat gacgcgaacg
      121 actcatgcgc atcgttgtcg gtgggagcgt cgctcacgtg tgcctctggc tcgggtccga
      181 aacgatcgtc gccgatcgca gtggacgaca tcgtaccgac cgcaacgctg cggggatgtg
      241 accgtgctca ggagctcctc gagataatga agtgccgatc cggccccggg gcgcgctagg
      301 gtgatggccc ctggcgacgt ggccgagtgg ttaggcagag gcctgcaaag ccttgtacac
      361 cggttcgaat ccggtcgtcg cctctccagg gcgcccgaag catccgtgtg gtgcggaggg
      421 cgaaggtgtc gggcgacacc gccgtggcga ttggtgtcaa ccgatcggtg cggtgctaag
      481 gtgtcgctca ccaaacgggc cgttggcgca gttggctagc gcacctgcat gacgcgcagg
      541 gggtcagggg ttcgaatccc ttacggccca caagagcgaa accccccccg agcgatcggg
      601 gggtttcgtc gttttcaggc gtgggcgggc actgcactcg acggcacgac ggcactcgac
      661 ggcacgacgg cgcacacatg tcgacggtgc ccacacgaca aagcggggtg gaatcgacca
      721 gtggccggct ccaccccgct gagcgggttc actcagttgt gaacgacgat caggcgtcgc
      781 cgacccggcg gcgacccatg gtcagcgcag caccgaggaa gatgagcccg gcgccgaaca
      841 cggcgagcca cacgttgtcg ctacccgtcg ctgccaattc tgctccgacc ttgccgttgc
      901 tgcccggcgc caccgtggtt gctgcccgga cgtcgttcgg aatcgtggtt gcgctgtcga
      961 ccacggcgac ggttgtcgtc ggggcggcac tggtgggcgc tacggccgtg gtggaggtcg
     1021 tggtggttgg ctcttcacag tctgcggcac gaccgaggtg ctgatgcgca gtgccgaggt
     1081 cgccagggaa caacagcgtg tggtcgaagc cggtgttcaa ctgctcgggg ttgcgtccgt
     1141 cggtgaggtc gaactgcacc cgacggcaca ccttggtgaa ctcgatcgtg taggtgccgg
     1201 cgcccttgaa cacgacgtcg agggtctcag acagggtctg gggccactgg ttggtcgtct
     1261 tcatgtcgta gatgaccgcg gtggccgcaa ccggcgaaca cagcggcgcg ggtgcgttga
     1321 tctcgagggt ccaggtggcg tcggtcggga tcgcacggtt ggaccactgg ggatcgaga
//
LOCUS       DAOOMA010000662         1369 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_127513_length_1369_cov_3.685693, whole genome shotgun
            sequence.
ACCESSION   DAOOMA010000662 DAOOMA010000000
VERSION     DAOOMA010000662.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 1369)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1369)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1369
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_127513_length_1369_cov_3.685693"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..454)
                     /locus_tag="PLP95_13670"
     CDS             complement(<1..454)
                     /locus_tag="PLP95_13670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB46897.1"
                     /translation="MSDDIPTDRASAANDAERAEGAEEVRLFGDPRSGLIDRRSPGTP
                     TPEGAAMARRATDIDSGVRLNRGTGFATFLLVSGWLLFGATLIAGGLIIYNAQEVGAF
                     SNPWNSTRLAVGIAVLSIGLTQSILMIALSLVMTQLQTLMRFRFRDAPT"
     gene            complement(496..>1369)
                     /locus_tag="PLP95_13675"
     CDS             complement(496..>1369)
                     /locus_tag="PLP95_13675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020379013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HPB46898.1"
                     /translation="SLPLLEAGSGLTGGVDFHLGYSPERIDPGNQRYRLENTPKVVSG
                     MNAESLAHVQGFFDRLVETTVPVSGTREAELTKLLENTFRHVNIALVNELAMFAKDLD
                     INVWEAIDAASTKPFGYMRFTPGPGVGGHCLPIDPSYLSWQIERRLGQTFRFVELAND
                     VNSHMPDYVVRRVQFLLNEAERSVKGSKVLVFGLAYKADTGDARETPSVPICELLTGL
                     GANVSVVDPHVEDRQFPDGVSRAEGTPQQIAEADIVLFLVDHSNFDTAAIVGNARLVL
                     DCRHRLEGPNVEHL"
BASE COUNT          226 a          411 c          502 g          230 t
ORIGIN      
        1 gtgtcggggc gtcgcgaaac ctgaaccgca tgagcgtctg caactgggtc atgaccagcg
       61 acaacgcgat catcaggatc gactgggtca agccgatgga caacacggcg atgcccaccg
      121 cgaggcgcgt cgagttccac gggttggaaa acgcgccgac ctcctgagcg ttgtagatga
      181 tgagcccgcc ggcgatgagc gtcgctccga acaacagcca tcccgacacg agcaggaagg
      241 tggcgaatcc cgttccgcgg ttgaggcgca cgccggaatc gatgtcggtg gcgcgccgtg
      301 ccatcgccgc gccctcgggc gttggagttc cgggcgagcg tcggtcgatg aggcccgagc
      361 gcgggtcgcc gaagaggcgg acctcctcgg cgccctcggc gcgctcggcg tcgtttgctg
      421 cgctagcgcg atcggtggga atgtcgtcgg acatcgcccg acaccctagt ggtggccgat
      481 cggcaggtgg tgggtctaca ggtgttcgac gttggggccc tcgaggcggt ggcggcagtc
      541 gagcacgagg cgggcgttgc ccacgatcgc tgcggtgtcg aagttcgagt gatcgacgag
      601 gaacaacacg atgtcggcct cggcgatctg ttgcggggtg ccctcggctc gggagacgcc
      661 gtcggggaac tgacggtcct cgacgtgggg gtcgacgacc gacacgtttg cgccgaggcc
      721 ggtgagcaac tcgcagatcg gcaccgacgg ggtttcgcgg gcgtcgccgg tgtcggcctt
      781 ataggcgaga ccgaacacca gcaccttcga ccccttcacc gaccgttcgg cctcgttgag
      841 gaggaactgc acccgccgca cgacatagtc gggcatgtgg ctgttgacgt cgttggccag
      901 ctcgacgaag cggaaggtct ggccgaggcg acgctcgatc tgccacgaca gatagctcgg
      961 gtcgatcggc aggcaatgcc cgccgacacc ggggccgggg gtgaagcgca tgtatccgaa
     1021 cggcttggtc gacgcggcgt cgatcgcctc ccatacgttg atgtcgagat ctttggcgaa
     1081 catggccaac tcgttgacga gcgcgatgtt gacgtggcgg aaggtgttct cgagcagctt
     1141 ggtgagttcc gcctcgcggg tgcccgacac cggcactgtg gtctcgacga ggcggtcgaa
     1201 gaagccctgg acgtgggcga gcgactcggc gttcattccc gacacgacct tcggggtgtt
     1261 ttcgaggcgg taacgctggt tgccggggtc gatgcgttcc gggctgtagc ccaggtggaa
     1321 gtcgacgccg ccggtgaggc ccgagccggc ttcgagcagc ggcagggac
//
LOCUS       DAOOMA010000663         1360 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_128856_length_1360_cov_3.564751, whole genome shotgun
            sequence.
ACCESSION   DAOOMA010000663 DAOOMA010000000
VERSION     DAOOMA010000663.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 1360)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1360)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1360
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_128856_length_1360_cov_3.564751"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..922
                     /locus_tag="PLP95_13680"
     CDS             <1..922
                     /locus_tag="PLP95_13680"
                     /inference="COORDINATES: protein motif:HMM:NF014065.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="bifunctional ornithine
                     acetyltransferase/N-acetylglutamate synthase"
                     /protein_id="HPB46899.1"
                     /translation="GAAVEGIAAQQLAVASTGVIGRRYPMEQMRSAIAGLSLPDTEAT
                     AADFAAAAQAIMTTDTHAKLVSAEVETLDGSTATLVGIAKGVGMIEPNMATLLTFFFT
                     DAELSAPALDAAFRSAMEVTFNALSIDTDTSTSDSAAIFANGAAGRVDPAAFGAALRS
                     VARDLVLMIAADGEGASKVIEVRVSGARDDAQAKLVAKAIVNSPLVKTAVHGSDPNWG
                     RIVMAIGKCDDQVDIDQDRVSVDIGDTRMYPSAATESERSDALARAEAHMAGDHVVLS
                     ANLGIAAGEFTVYGCDLTDGYIRINADYTT"
     gene            919..>1360
                     /gene="argB"
                     /locus_tag="PLP95_13685"
     CDS             919..>1360
                     /gene="argB"
                     /locus_tag="PLP95_13685"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020376860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HPB46900.1"
                     /translation="MSNDPTSSTQPGNDAPSNNQGGSGQSRQVDVLLQALPYIQRFRS
                     ATVVVKFGGNAMTSPELFDQFAQDIVMMHSVGIRPVVVHGGGPQIGQWLDRLGKSSEF
                     VDGQRVTDAETLEVVQMVLVGKVNSDIVSALNAHGPVAVGLSGQD"
BASE COUNT          246 a          455 c          455 g          204 t
ORIGIN      
        1 aggagcggcg gttgagggca tcgccgcgca acaacttgcg gtcgcctcca ccggggtgat
       61 cggacggcgc tacccgatgg agcagatgcg atccgcgatc gccgggctgt cgctgcccga
      121 tacggaggcc acggctgccg acttcgccgc agccgcgcag gcgatcatga ccaccgacac
      181 ccacgcgaag ctcgtttcgg ccgaggtgga aaccctcgac ggttcgaccg ccaccctcgt
      241 cggtatcgcc aagggggtcg gcatgatcga gcccaacatg gcgaccctgc tcaccttctt
      301 cttcaccgac gccgaactca gcgccccggc cctcgacgcc gcgttccgtt ccgcgatgga
      361 ggtcacgttc aacgcactca gcatcgacac cgacacctcg acgagcgact cggcggccat
      421 cttcgccaac ggcgcggccg gccgtgtcga tccggcggcc ttcggcgccg cgcttcgctc
      481 ggtggcccgg gacctggtgt tgatgatcgc ggccgacggc gaaggagcct cgaaggtgat
      541 cgaggtgcgg gtgagcggcg cacgagacga tgcccaggcg aaattggtgg ccaaagcgat
      601 cgtgaattcg ccgctggtga agacggcggt gcacggctca gacccgaact ggggccgcat
      661 cgtgatggcc atcggcaagt gcgatgacca ggtcgacatc gaccaggacc gggtgtcggt
      721 cgacatcggc gacactcgca tgtatcccag cgccgcgacc gaatccgaac gcagcgacgc
      781 cctcgcacgg gcagaggccc acatggccgg cgaccacgtc gtgttgtccg cgaacctggg
      841 cattgccgcg ggcgagttca cggtgtacgg atgcgacctc accgacggct acatcaggat
      901 caacgcggac tacacgacat gagcaacgac ccgacctcaa gcactcaacc cggaaacgac
      961 gccccctcga acaaccaagg aggcagcggg cagagccgcc aggtcgacgt gttgttgcag
     1021 gcgcttccct acatccagcg cttccgttcg gcgacggtcg tggtgaagtt cggcggcaat
     1081 gcgatgacgt cgcccgagct gttcgaccag ttcgcccagg acatcgtcat gatgcactcg
     1141 gtgggcatcc gcccggtcgt cgtgcacggc gggggacccc agatcgggca gtggctggac
     1201 cgtctcggca aatcgagcga gttcgtcgac ggccaacggg tgaccgacgc cgagaccctc
     1261 gaggtcgtgc agatggtgct cgtgggcaag gtgaactccg acatcgtcag cgcgttgaac
     1321 gctcacgggc cggttgcggt gggcctgtcc gggcaggacg
//
LOCUS       DAOOMA010000664         1353 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_129882_length_1353_cov_3.155624, whole genome shotgun
            sequence.
ACCESSION   DAOOMA010000664 DAOOMA010000000
VERSION     DAOOMA010000664.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 1353)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1353)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1353
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_129882_length_1353_cov_3.155624"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(349..777)
                     /locus_tag="PLP95_13690"
     CDS             complement(349..777)
                     /locus_tag="PLP95_13690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB46901.1"
                     /translation="MSTTFAELLGAHNIIIPDETLTELEVPVLSGPQRQGDVGIFPRS
                     QVSAAELSHMTAIPTTCVQVVRGEATGNTHLLHPAGRCWWRPIESKAGSVLLGVLHVD
                     DDAECFLIHTDEHGANGIAPGSYEIRGKRELADEIRRVAD"
     gene            complement(791..>1353)
                     /locus_tag="PLP95_13695"
     CDS             complement(791..>1353)
                     /locus_tag="PLP95_13695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB46902.1"
                     /translation="QSAGWWWPHREFVMVCETPDMIVREQVGPRGWGSHQLHNDRGPA
                     IRWRDGWALHFWHGTRVPAWVIDGPTVEKINAETNSEIRRCAIEAYGWDLYLEHLGAN
                     PVDVADDPGNPGHTLRLYDVPNSRELYDGNDVRLLVMDNASRDRDGSRRTYAETVPAT
                     IATCVAAAAWQFDVPETTYRELVRAT"
BASE COUNT          232 a          376 c          470 g          275 t
ORIGIN      
        1 tgggcgtgcg tccaaacccg gtcgacacga ctgcgagcat atgatcaaaa atcggttggg
       61 gtggggtagg cgtgtagatg tgcgctctcg tgttcagcgt catgactggc actcctcggg
      121 tgggaggatg acgaacgagt cgccgaacgg ttggatgcgc cagccgggtg gcactgtcaa
      181 cgcttcgatc gttacagcaa cgagagcggg ctcaaactcg acaacggttg cagaatccgt
      241 cattagacag tcctttcaat tgtgtgattc ggaaaatatc tatggtgcga gcgagggaac
      301 gcacagccgg tagggtggtg gacgggtcga gtccgagaat caacacactc aatccgctac
      361 gcggcgaatc tcgtcggcga gttcacgctt cccgcggatc tcataggacc ccggtgcgat
      421 gccgttcgct ccgtgctcat cggtgtgaat cagaaagcac tcggcgtcgt cgtcgacgtg
      481 cagcacgccg agcaggaccg atccggcttt ggattcgatc ggacgccacc agcatcgacc
      541 ggcagggtgt agcagatggg tgttcccggt tgcttcgccg cgcacaacct gtacgcacgt
      601 cgtcgggatc gcggtcatgt gtgagagttc ggcggcggac acctgcgagc gggggaagat
      661 gccgacgtcg ccttggcgtt gaggccccga cagcaccggc acctcgagtt cggtgagggt
      721 ttcgtcgggg atgatgatgt tgtgggcgcc gaggagttcg gcgaacgtgg tggacattgg
      781 tgttcctctt tcaggttgcg cgaacgagtt cgcggtaggt tgtttcgggt acgtcgaact
      841 gccaggccgc tgctgccaca caggtggcga tcgtggcggg cacggtttcg gcgtaggtgc
      901 ggcgtgagcc gtctcggtca cgtgacgcgt tgtccatgac gaggagtcgg acgtcgttgc
      961 cgtcgtaaag ctctcggctg tttggcacgt cgtagagccg cagtgtgtgg cccgggttgc
     1021 cgggatcgtc ggcaacgtcc acggggttcg ctccgaggtg ttcgaggtac agatcccagc
     1081 cgtacgcttc gatcgcacac cggcggatct cggagtttgt ttcggcgttg atcttctcga
     1141 cggtgggtcc gtcgatgacc caggcgggga ctcgtgtgcc gtgccagaag tggagtgccc
     1201 agccgtcacg ccagcggatg gcggggcctc ggtcgttgtg gagctggtgc gacccccagc
     1261 ctcgaggccc gacctgctcc cggacaatca tgtccggggt ctcgcacacc atgacgaact
     1321 ctcggtgcgg ccaccaccaa ccagcggatt gag
//
LOCUS       DAOOMA010000665         1349 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_130428_length_1349_cov_5.112056, whole genome shotgun
            sequence.
ACCESSION   DAOOMA010000665 DAOOMA010000000
VERSION     DAOOMA010000665.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 1349)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1349)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1349
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_130428_length_1349_cov_5.112056"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..254)
                     /locus_tag="PLP95_13700"
     CDS             complement(<1..254)
                     /locus_tag="PLP95_13700"
                     /inference="COORDINATES: protein motif:HMM:NF014790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HPB46903.1"
                     /translation="MTIAITDDHRTLAETADAFLTRVDSKGAARALLTTDDETLPTFW
                     SDLAELGWLGLHLPEEHGGSGYGAPEQVVIIEALARHAAPG"
     gene            complement(314..>1349)
                     /locus_tag="PLP95_13705"
     CDS             complement(314..>1349)
                     /locus_tag="PLP95_13705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015439742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HPB46904.1"
                     /translation="LGRMAALDAGYDTLASGVTLDRFCGSGITVANLGAATIMSGMEH
                     VVIAGGSEMMSSYRENSPRTPMPFLDSGNEHLRAIHPQPHQGVSADVIATLEKIPRSA
                     LDELSLESQRRAAVAQAEGRFDKSLIAVHHPDGSLALDRDEYPRPQTTLESLAGLTPA
                     FTTLADHPLDDQGTTFRSLVHQKFPGLDIEHVHHAGNSSGVVDGAAAILMTSDAYAKA
                     NGLTPRARIRAFANVGDDPTLMLNAPVPAARKVLDKAGLTLDDIDLFEVNEAFAVVAE
                     KFIRDLDIDRNKINVNGGAIALGHPIGATGAMLIGTVLDELERTDGTFGLVTMCAAGG
                     MAPAVIIERI"
BASE COUNT          193 a          390 c          527 g          239 t
ORIGIN      
        1 ccgggagcgg cgtggcgtgc gagcgcctcg atgatcacga cctgttcggg ggcaccgtaa
       61 cccgatccgc cgtgttcctc gggaaggtgc agtccgagcc agccgagctc ggccaggtcg
      121 gaccagaacg tcggcagggt ctcgtcgtcg gtggtcagca gcgcgcgggc cgcgcccttg
      181 gaatcgacgc gggtcaggaa ggcatccgcg gtctcggcga gggtgcggtg gtcgtcggtg
      241 atggcgatgg tcatggaggt acctctcgtt cggttcggtt cgttcggttc gatctggttg
      301 tcggcggtgt gggtcagatg cgctcgatga tgaccgccgg ggccatgccg cccgcagcgc
      361 acatcgtgac gagcccgaag gtgccgtcgg tgcgttcgag ttcgtccaac acggtgccga
      421 tgagcatcgc cccggtggcg ccgatcgggt ggccgagcgc gatggcgccg ccgttaacgt
      481 tgatcttgtt gcggtcgatg tcgaggtcgc ggatgaactt ctcggccacg acggcgaagg
      541 cctcgttcac ctcgaacagg tcgatgtcgt ccaaggtcag cccggccttg tcgagcacct
      601 tgcgtgccgc gggcaccggg gcgttcaaca tcagcgtcgg atcgtcgccg acgttggcga
      661 aggcccggat gcgggcgcgg ggcgtcagcc cgttggcttt cgcgtaggcg tcggaggtca
      721 tgaggatcgc ggcggcgccg tcgaccacac cggagctgtt gccggcgtga tgaacgtgtt
      781 cgatgtcgag gccggggaac ttctggtgca ccagcgatcg gaaggtggtg ccctgatcgt
      841 cgagcgggtg gtcggccagg gtcgtgaacg ccggggtcaa tccggcgagc gactcgagcg
      901 tcgtctgggg acgcgggtac tcatcgcggt cgagggcgag ggagccgtcg gggtgatgga
      961 cggcgatgag tgacttgtcg aaacgcccct cggcctgggc gaccgcggcg cggcgttggg
     1021 attcgaggga gagctcgtcg agggccgaac gcgggatctt ctcgagcgtc gcaatgacgt
     1081 cggccgatac gccctgatgg ggttgggggt ggatggcacg aaggtgttcg ttgcccgagt
     1141 cgaggaaggg catcggcgtg cgcggcgagt tctcgcgata gctcgacatc atctcggatc
     1201 ctccggcgat gacgacgtgt tccatcccgc tcatgatggt ggcggcgccg aggttggcca
     1261 cggtgatgcc cgacccacag aagcggtcga gggtgacccc cgaggcgagg gtgtcgtagc
     1321 cggcgtcgag cgcggccatg cgcccgagg
//
LOCUS       DAOOMA010000666         1344 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_131193_length_1344_cov_3.530644, whole genome shotgun
            sequence.
ACCESSION   DAOOMA010000666 DAOOMA010000000
VERSION     DAOOMA010000666.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 1344)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1344)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1344
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_131193_length_1344_cov_3.530644"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..671)
                     /locus_tag="PLP95_13710"
     CDS             complement(<1..671)
                     /locus_tag="PLP95_13710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB46905.1"
                     /translation="MDLATAKDHIRSTVDANRHRLIEVSHDLWEHPELCFEEHRAHDL
                     LTAELEAAGFEVERGAFGLDTAFRARAGSPSATGPSVGIVLEYDALPVVGHACGHNII
                     AAAGLGAAIAAAEVVERFDARLVVYGTPAEEGGAGKVHMIDRGAFRDVDVAMMVHPAD
                     VDLNAFWAIAIQELHVDYHGRAAHAAAAAHLGLNALDAAVLGYMNVAALRQHIAGNER
                     VHGIFT"
     gene            complement(743..>1344)
                     /locus_tag="PLP95_13715"
     CDS             complement(743..>1344)
                     /locus_tag="PLP95_13715"
                     /inference="COORDINATES: protein motif:HMM:NF012335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HPB46906.1"
                     /translation="ADMIEFAAHLAECAAYPVALTSAWRMLRRGRFEPGQTVLVTGIG
                     GGVATAAQMLVQHLGGRVFTTSRDEYKRQRSVELGAEDSFDSTQRYPLKADIVVDSIG
                     PATWDNCVGALKPGGRLVTCGGTTGQIVELNLPRLFFKHHEILGSTLGSPEEFAYVTG
                     LMNDGLAVVIDHEYPLDQYPQAVERIRSGEQLGKLVIHH"
BASE COUNT          198 a          436 c          471 g          239 t
ORIGIN      
        1 gtgaagatgc cgtgcacccg ttcgttgccg gcgatgtgct ggcgcagggc cgccacgttc
       61 atgtatccca acacggcggc atcgagggca ttgaggccca ggtgcgcagc ggccgcggcg
      121 tgggcggccc tgccgtggta gtcgacgtgg agctcctgga tggcgatcgc ccagaaggcg
      181 ttgaggtcga cgtccgcggg gtgcaccatc atggcgacgt cgacgtcgcg aaacgcgccc
      241 cggtcgatca tgtgcacctt gccggcgccg ccctcttcag ccggggtgcc gtagacgacg
      301 agccgggcgt cgaagcgttc gacgacctct gctgcggcga tggccgcgcc gaggccggcg
      361 gccgcgatga tgttgtgccc gcacgcgtgg ccgacgacgg gcagggcgtc atattccaac
      421 acgatcccga cgctcggccc ggttgctgac ggcgatccgg cgcgggcccg gaacgcggtg
      481 tcgagcccga aggctccgcg ttcgacctcg aacccggccg cctcgagttc ggcggtcagc
      541 aggtcgtgcg ctcgatgttc ctcgaaacac agctccgggt gctcccacag gtcgtgggac
      601 acctcgatga gccgatgacg gttcgcgtcg accgtcgagc ggatgtggtc cttggcagtg
      661 gcgaggtcca tgtcgccagc ctaggtctgt gagcgcggct gaccgcttct gtgcggtgct
      721 tcagcggtga ttgagcgctg tttcagtggt gaatgaccag cttgccgagc tgttctccgc
      781 tacggatccg ctcgaccgct tgcgggtatt ggtcgagtgg atattcgtgg tcgatgacca
      841 ccgccagtcc gtcgttcatc aacccggtga cgtaggcgaa ctcctcgggt gaaccgaggg
      901 tggagcccag aatctcgtgg tgcttgaaga acagccgggg caggttcagc tcgacgatct
      961 gtccggtggt gccgccgcag gtgacgagtc gtccccccgg tttcagcgca ccgacacagt
     1021 tgtcccacgt cgccggaccg atggaatcga ccacgatgtc ggccttgagc gggtaccgct
     1081 gggtcgaatc gaagctgtcc tcggccccga gttccaccga ccgctggcgc ttgtattcgt
     1141 cgcgcgaggt cgtgaagact cgcccgccga ggtgttgcac cagcatctgc gcggcggtgg
     1201 ccactccccc gccgattccg gtgacgagca ccgtctgtcc gggttcgaaa cggcctcggc
     1261 gcagcatccg ccacgccgag gtgagcgcga ccgggtacgc ggcgcactcc gcgaggtgcg
     1321 cggcgaactc gatcatgtcg gccg
//
LOCUS       DAOOMA010000667         1326 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_134042_length_1326_cov_3.878049, whole genome shotgun
            sequence.
ACCESSION   DAOOMA010000667 DAOOMA010000000
VERSION     DAOOMA010000667.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 1326)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1326)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1326
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_134042_length_1326_cov_3.878049"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..928
                     /locus_tag="PLP95_13720"
     CDS             <1..928
                     /locus_tag="PLP95_13720"
                     /inference="COORDINATES: protein motif:HMM:NF022732.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3089 domain-containing protein"
                     /protein_id="HPB46907.1"
                     /translation="ISSHPSSMRVSDWFASEDEEGYVARYQAAPLTSRCRVFAPLYRQ
                     WTLASIGARVGGDVPDDEVNPYDDVLAAWRHYLATDNDGRGVVLIGHSQGSMHLTRLI
                     QEEIEPDESASRLLIAAYLAGTSIQVPDGADVGATFSSTPLCREETDTGCVVTWSSYR
                     SEEPESAGSIFGRHFDGTVAACNPPSSLRGTRSVTSPLMPAARNASILSSLLPAVPED
                     ASWFDGAVIDTTYVALDDFVFAECRDTETKNAAMITVHADPTDPRADDITGDLSAEWG
                     LHLVDVSLFLGDIIDLISTQFEAWESAPSASG"
     gene            complement(1079..>1326)
                     /locus_tag="PLP95_13725"
     CDS             complement(1079..>1326)
                     /locus_tag="PLP95_13725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB46908.1"
                     /translation="GAAVTAVGVAVDPVSANEAAAKPPTPPATSAAATVTAAAPRRAA
                     RGWDFADRPRGAADSAASREAEGASLGGAPKTDDGAE"
BASE COUNT          211 a          483 c          412 g          220 t
ORIGIN      
        1 catcagcagc cacccctcgt cgatgcgggt ctccgactgg ttcgccagcg aggacgaaga
       61 gggctacgtg gcccgatacc aggccgcacc gttgaccagc cgctgtcggg tattcgcccc
      121 gttgtaccga cagtggaccc tggcatcgat cggtgctcgg gtcggtggcg atgttcccga
      181 cgacgaggtc aacccctacg acgacgtcct cgccgcctgg cgccactacc tcgcgaccga
      241 caacgacggg cgaggggtcg tgttgatcgg acactcgcag ggatcgatgc acctgacgcg
      301 attgattcaa gaggagatcg agccggacga gtcggcctca cgactcctca tcgccgccta
      361 cctggccggc acctcgatcc aggtccccga cggcgccgac gttggagcga cgttctcctc
      421 gacgccgttg tgtcgggagg aaaccgacac cggttgtgtc gtcacctggt cctcgtaccg
      481 aagcgaggag cccgaatcgg cgggctccat cttcggccgc cacttcgacg gcacggtcgc
      541 tgcgtgcaac cccccgtcgt cgttgcgcgg gacgcgctcg gtgacctccc cgttgatgcc
      601 ggccgcccgc aacgcctcga tcttgtcctc gctgctcccg gcggtacccg aagatgcctc
      661 atggttcgat ggagcggtca tcgacaccac ctatgtcgca ctcgacgact tcgtgttcgc
      721 cgagtgccga gataccgaaa cgaagaacgc cgcgatgatc acggtgcacg ccgatcccac
      781 cgacccgcgc gctgatgaca tcaccggcga cctgtctgcc gagtggggcc tgcacctcgt
      841 cgacgtgagc ctctttctcg gcgacatcat cgacctgatc agcacacagt tcgaggcgtg
      901 ggaatcagcg ccgtccgcat ccggctgaac gttcccacag gcacacagcc gactttcagg
      961 gtcttcggag gaagctccaa ggttcgcgga ggaaagtcgt tcccatgaac gaatctccct
     1021 ttccgcctca tcagccctgg gtgggctccc cgcctcccta cccgccgaac gaaccgccct
     1081 attcggcacc gtcgtcggtc ttgggagcgc cgcccagcga ggcgccttcc gcttcgcgcg
     1141 atgccgccga gtccgcggca ccgcgaggcc ggtcggcgaa atcccaaccg cgggccgctc
     1201 ggcgcggggc ggcggccgtg acggtcgccg ccgcgctcgt cgccggcgga gtcggcggct
     1261 tcgcggcggc ttcattcgcc gaaaccggat cgaccgcgac gcccaccgcg gtgaccgcgg
     1321 cacctc
//
LOCUS       DAOOMA010000668         1326 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_134057_length_1326_cov_3.467349, whole genome shotgun
            sequence.
ACCESSION   DAOOMA010000668 DAOOMA010000000
VERSION     DAOOMA010000668.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 1326)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1326)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1326
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_134057_length_1326_cov_3.467349"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..401
                     /gene="lexA"
                     /locus_tag="PLP95_13730"
     CDS             <1..401
                     /gene="lexA"
                     /locus_tag="PLP95_13730"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012230868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HPB46909.1"
                     /translation="SGIAAGIVSVPLVGDVAAGYGVIAEENIEDTLVLPAQFTGSGDL
                     FMLRVRGESMIEAGIFDGDFVVVRQQATAESGDTVVAGVDDELGTVKNIRFEGSEVVL
                     SPANSTMEPRRFPADSVEIYGKVVTVLRKL"
     gene            complement(628..>1326)
                     /locus_tag="PLP95_13735"
     CDS             complement(628..>1326)
                     /locus_tag="PLP95_13735"
                     /inference="COORDINATES: protein motif:HMM:NF026139.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DapH/DapD/GlmU-related protein"
                     /protein_id="HPB46910.1"
                     /translation="RLHLLSTCKVRPHGLSLEGQFGLLNNVVWTDRGPFDPDTFEATR
                     ARLRAAGEPVTVLGIDKFPNLVDYVVPAGVRIAHGARVRLGAYLGEGTTVMHEGFVNF
                     NAGTEGPAMVEGRISAGVFVGANTDIGGGASIMGTLSGGGKEVVSIGSGSLLGANAGI
                     GISLGDNCVVEAGLYVTAGTVVELPDGSTVKAVALSGRSDMLFRRDSRRGAVVMLPQT
                     AEWGGLNTDLHSNA"
BASE COUNT          219 a          466 c          433 g          208 t
ORIGIN      
        1 cgtccggcat tgccgcaggc atcgtgtcgg tgccgctcgt cggcgatgtc gctgcaggct
       61 acggcgtgat cgccgaggag aacatcgaag acaccctcgt tctgccggcg cagttcaccg
      121 gctccggaga cctgttcatg ctgcgggtcc gcggcgaatc gatgatcgaa gccggcatct
      181 tcgacggcga cttcgtcgtg gtgcgccagc aggccaccgc ggaaagcggc gacacggtgg
      241 tcgccggggt cgacgacgaa ctgggcacgg tcaagaacat ccgattcgag gggtccgagg
      301 tcgtgttgtc gcccgccaac tcgaccatgg aaccccgccg tttccccgcc gattcggtgg
      361 agatctacgg caaggtcgtg accgtcctgc gcaagctgta acgacggctc tccccgtcgt
      421 cgcgaccgct cccggccccg caagcgccgc ggtgcgagcg gtcgcgggca ccagacgcaa
      481 gcgcgatgac cgttcgaggc cacacctcgg gcggtcatcg tcgcgccggg cgctcgtcgc
      541 atcgggaact caggcgctcg ggtgcaccgg gcgctcagac tcgtcgggcg cctaggcgaa
      601 ccgaccgccc agaggcgaac caggtgctca ggcgttgctg tgcaggtcgg tgttcaaccc
      661 gccccattcg gcggtttggg gcagcatcac gacggctccg cgccggctgt cgcggcggaa
      721 caacatgtcg ctgcgaccgc tcagcgccac cgccttcacc gtcgatccgt cgggcagttc
      781 caccacggtc ccggcggtga cgtagaggcc ggcctcgacc acgcagttgt cacccagact
      841 gatgccgatt ccggcattgg cgccaagcag gctgcccgaa ccgatcgata ccacttcttt
      901 cccgcctccc gacagggtgc ccatgatcga cgcgcctcca ccgatgtcgg tattcgcgcc
      961 gacgaaaacc cccgccgaga tacgcccctc caccatggcc ggaccctcgg ttccggcatt
     1021 gaagttcacg aacccctcgt gcatcacggt cgttccctcg ccgagatagg cgccgagacg
     1081 aacccgagct ccgtgggcga tacgcacacc ggccggcacg acgtagtcga cgaggttcgg
     1141 aaacttatcg attcccagca cggtcacggg ctcgccggcc gcgcgcagcc gggcgcgggt
     1201 ggcttcgaag gtgtcagggt cgaacgggcc gcgatcggtc cacacgacgt tgttcaacaa
     1261 gccgaactgc ccttcgaggc tcaggccgtg ggggcgcacc ttgcaggtcg acagcaggtg
     1321 caggcg
//
LOCUS       DAOOMA010000669         1320 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_134962_length_1320_cov_3.836364, whole genome shotgun
            sequence.
ACCESSION   DAOOMA010000669 DAOOMA010000000
VERSION     DAOOMA010000669.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 1320)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1320)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1320
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_134962_length_1320_cov_3.836364"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..936)
                     /locus_tag="PLP95_13740"
     CDS             complement(<1..936)
                     /locus_tag="PLP95_13740"
                     /inference="COORDINATES: protein motif:HMM:NF017760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HPB46911.1"
                     /translation="MSSPTATTSRRTAPRPGSPRAAFASRAFRWVFLGTFASNIGTWM
                     QNVALLAFADQLTHDATFVGIVTMAQLGPMLVLSPIGGVLADRFDRKVLMIGAATVQA
                     SMSAALAFAAMSDDPSLLLIVMCVLGIGIASAINGPAAQATTPALVGRENLAGAVAVN
                     SAQMNASRVLGPILALIPFLETPSTVFFVNASTYVFVIAAIALVKFDSKPPHDPNREA
                     SIRQFIAGVRAAKANKVVGRGLATVSIYSLFSLAYIYQMAGFSREELGLAGQAYRLLF
                     GTFGLGAALGAIAVGTRLAQVDRTILVRVGLGGFGV"
     gene            complement(1054..1127)
                     /locus_tag="PLP95_13745"
     tRNA            complement(1054..1127)
                     /locus_tag="PLP95_13745"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1091..1093),aa:Val,seq:cac)
BASE COUNT          242 a          417 c          469 g          192 t
ORIGIN      
        1 cacgccgaag ccgccgaggc cgacgcgcac gaggatcgtg cgatcgacct gcgccagccg
       61 ggttccgacc gcgatggccc ccagcgcggc gccgaggccg aaggttccga acaacagccg
      121 gtaggcctgg cctgccaggc cgagctcctc acgactgaag ccggccatct ggtagatgta
      181 cgcgagcgag aacagcgaat agatcgacac ggttgcgagc ccgcggccga ccaccttgtt
      241 ggccttcgcg gcgcgaaccc cggcgatgaa ttggcgaatc gaggcctcgc ggttcgggtc
      301 gtgcgggggc ttggagtcga atttcaccag cgcgatcgcg gcgatgacga acacataggt
      361 cgacgcgttg acgaagaaca cggtgctcgg agtttccagg aacgggatga gcgccaggat
      421 cgggccgagg acccgcgatg cgttcatctg tgccgagttc accgcgaccg cgccggcgag
      481 gttctcgcgc ccgacgagtg cgggggtggt ggcctgggcg gccgggccgt tgatggccga
      541 ggcgattccg attccgagca cacacatcac gatgagcaac agcgacgggt catccgacat
      601 cgccgcaaac gccagcgccg cactcatcga cgcctgcacg gttgccgcgc cgatcatcag
      661 caccttgcgg tcgaagcggt cggcgaggac acccccgatc ggggacaaca ccagcatcgg
      721 gccgagttgg gccatggtga cgatgccgac gaaggtcgcg tcgtgggtga gctgatcggc
      781 gaaggccaac agcgcgacgt tttgcatcca ggtgccgatg ttcgacgcga aggtaccaag
      841 aaacacccag cgaaacgccc gggacgcgaa cgccgcacga ggcgaaccgg gtcgcggtgc
      901 ggttcgcctg gaggttgtcg cggtcgggga actcacgcgt cgagtctccc cggcgctgtg
      961 gtccgggggc gaatctgagc tcatgcgctg cgctcgccga ggcggccgag gtggccgaca
     1021 accctcccgc cgaggttgcc gtggtgttcg tagtgggcga tggcagactc gaactgccga
     1081 cctcacgcgt gtgaagcgtg cgctctaacc aactgagcta aacgcccgat agcggccgca
     1141 atgttagcag agcggcgcgc cgctcgatcc aatcacgacc acgactgaag gtcggcgagg
     1201 tggcgagagc catgggggga gccgtggcgg gcgaggttct cagaccccga gggagcggcc
     1261 gatgacctct ttcatgatct cggtggtgcc gccgtagatg cgctgcacgc gcgcatcggt
//
LOCUS       DAOOMA010000670         1319 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_135250_length_1319_cov_1.570411, whole genome shotgun
            sequence.
ACCESSION   DAOOMA010000670 DAOOMA010000000
VERSION     DAOOMA010000670.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 1319)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1319)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1319
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_135250_length_1319_cov_1.570411"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..491)
                     /locus_tag="PLP95_13750"
     CDS             complement(<1..491)
                     /locus_tag="PLP95_13750"
                     /inference="COORDINATES: protein motif:HMM:NF013584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HPB46912.1"
                     /translation="MPDDLHLIDAVGQAALVRDGLATPAELTDAAIARIEATNPTLNA
                     VISERFEAARAEAASDLPDGPFRGVPILLKDLGAAMAGEIQHAGSSLLKALDYRAPAD
                     SHLVRRLRAAGFVILGRTNTPEFGSTCTTEPVSYGATHNPWNVEHSSGGSSGGSAAAV
                     AAR"
     gene            545..>1319
                     /locus_tag="PLP95_13755"
     CDS             545..>1319
                     /locus_tag="PLP95_13755"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HPB46913.1"
                     /translation="MSATVTDLPDELDRRGERRTNVQSVNRRGAALIARFVRAHPMPM
                     IVSVLGAVLFAASAVGSTVAIGSVTDTVITPAFRQGAGTLTSGTVWAGVGLIVMVGFV
                     RGMSIVVRRYFAAILEARMQATLRRGVVDKYLTAPLSFHHSRPTGELLAHADADVSGT
                     TMVIKPLPFSIGVIALVVFALVSLIEVDWAFTVIALGLFPMLTLLNKVYARRAEVPSA
                     LVQQRLGDVSAVAHESFDGALVVKTLGIEQAESERFALAS"
BASE COUNT          197 a          439 c          459 g          224 t
ORIGIN      
        1 atgcgggcgg cgaccgctgc ggccgaccca cccgacgagc caccggagga atgctcaacg
       61 ttccaggggt tgtgggtggc cccgtaggac accggctcgg tggtgcacgt cgacccgaac
      121 tccggggtgt tggttcggcc caggatgacg aagccagccg cccgaagtcg acgaacgagg
      181 tggctgtcgg ccggcgcccg gtagtcgagc gccttcagca ggctcgaacc ggcgtgttgg
      241 atctcgccgg ccatcgccgc gccgaggtcc ttgagcagga tcggtacgcc gcggaacggc
      301 ccgtcgggaa ggtcgctcgc cgcctccgcc cgcgcagcct cgaaccgctc ggagatcacc
      361 gcgttgaggg tcgggttggt cgcctcgatc cgagcgatgg ctgcatcggt cagctcggcc
      421 ggcgtcgcta aaccgtcgcg gacgagcgca gcttggccga ccgcgtcgat caggtggagg
      481 tcgtcgggca tgcccgcaaa ctactggtga tcgaatcgat cgtctcggta gcctcgccgt
      541 cgcgatgagt gcaacggtca ccgatctacc cgacgaactc gatcggcggg gcgaacggag
      601 aaccaacgtc cagagcgtca accgtcgcgg tgccgcactg atcgcccgct tcgtgcgcgc
      661 ccacccgatg ccgatgatcg tctcggtgct cggcgcggtc ctgttcgcgg cgagcgcggt
      721 cggctcgacg gttgcgatcg gctctgtgac cgacaccgtg atcacgccgg cgttccggca
      781 gggagccggc acgctcacat cagggaccgt gtgggccggg gttgggttga tcgtcatggt
      841 cggattcgtg cggggaatgt cgatcgtggt ccgccgctac ttcgcggcga tactcgaagc
      901 gcggatgcag gcgacactgc gccgcggcgt ggtcgacaaa tacctgaccg cgccgctctc
      961 gtttcaccac agccgcccga ccggcgaact gctcgcccac gccgacgccg acgtctccgg
     1021 caccacgatg gtgatcaagc cgctgccctt cagcatcggc gtgatcgcgc tcgtcgtgtt
     1081 cgctctggtc agcctcatcg aggtcgactg ggcgttcacc gtgatcgcgc tcggactgtt
     1141 cccgatgctg acgctgctca acaaggtgta cgcacgccgt gccgaggtgc cgtcggcgtt
     1201 ggtccagcag cgactgggtg acgtgtcggc cgtcgcgcac gagagcttcg acggagcgct
     1261 ggtcgttaag acgctcggca tcgaacaggc cgagtcggag cggtttgcct tggcctccg
//
LOCUS       DAOOMA010000671         1271 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_143373_length_1271_cov_3.350329, whole genome shotgun
            sequence.
ACCESSION   DAOOMA010000671 DAOOMA010000000
VERSION     DAOOMA010000671.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 1271)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1271)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1271
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_143373_length_1271_cov_3.350329"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..846
                     /locus_tag="PLP95_13760"
     CDS             <1..846
                     /locus_tag="PLP95_13760"
                     /inference="COORDINATES: protein motif:HMM:NF021379.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2079 domain-containing protein"
                     /protein_id="HPB46914.1"
                     /translation="GPLGNNSTEVVVGAVTDPGPFADTVLCHGFWDGSLHEADPGRAD
                     IVCPSGQILEEVYPPPRGFATLMQPFGYVGLLSPILLLVGLPQHLVNSATMQPFTWDL
                     RWHYALMPFAGVLLATVWSVVKRKRAIVAWALISMLVVGVVTTHDRGIGPWGADFSRG
                     YWRVVDDPTNQVYREVMAEIDGDDVVSTNYFFVPHLSHREYIYTFPNPWRLSNYGAAG
                     STVEPPDPATVDVVLIDRARLNAADQMLFDEVRNSGDFEVVTHRVATPPDRVADLYLL
                     RRRAP"
     gene            complement(851..>1271)
                     /locus_tag="PLP95_13765"
     CDS             complement(851..>1271)
                     /locus_tag="PLP95_13765"
                     /inference="COORDINATES: protein motif:HMM:NF019869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HPB46915.1"
                     /translation="ASASAANALARAVSVLQRLFAPFLPFATEEAWSWWMQGSVHGAP
                     WPTVAELDVEAAATELPVLAVVENVLRDVRRAKSEAKVGMKADVERVVVSDDSAHLAA
                     LELVAADLREALRIATLDTTPGASYSIDVTLAPPPAE"
BASE COUNT          192 a          419 c          430 g          230 t
ORIGIN      
        1 ggccccctcg gcaacaattc gaccgaggtg gtggtcggcg cggtgaccga tcccgggccc
       61 ttcgccgaca ccgtgctgtg tcacggattc tgggacgggt cgcttcacga agcagaccct
      121 ggtcgcgccg acatcgtgtg cccgtccggc cagatcctcg aggaggtgta ccctccgcct
      181 cgaggattcg cgacgctcat gcagccgttc ggatacgtcg ggctactcag tcccatcctg
      241 ttgctggtcg ggctgcccca gcacctcgtc aactcggcca cgatgcagcc attcacctgg
      301 gaccttcgct ggcactacgc gctcatgccc tttgccgggg tgctgttggc caccgtgtgg
      361 agcgtggtga aacgcaaacg cgcgatcgtc gcttgggcgc tgatctcgat gctggtggtc
      421 ggcgtcgtca cgacccatga ccggggtatc ggcccgtggg gggccgactt ttcgcggggg
      481 tactggcggg ttgtcgacga tccgaccaat caggtgtacc gcgaggtcat ggccgaaatc
      541 gacggcgacg acgtcgtgtc gactaactat ttcttcgtgc cacacctctc tcatcgcgag
      601 tacatctaca cgtttccgaa cccgtggcgg ctgtcgaact acggagctgc aggctcgacc
      661 gtcgagcccc ccgacccggc gaccgtggac gtggtgctga tcgaccgtgc gcgcttgaac
      721 gccgcagatc agatgttgtt cgacgaagtg cgcaacagcg gcgatttcga ggtggtgacc
      781 caccgggtgg ccacgccgcc cgatcgcgtt gccgatctct acctgttgag gcgtcgcgcc
      841 ccctgacgcg ctattcggcg ggcggcggcg cgagggtgac gtcgatcgag tagctggccc
      901 cgggtgtggt atcgagcgtt gcgatgcgca acgcttcgcg caggtcggcg gcgacgagtt
      961 cgagcgcggc gaggtgcgcc gagtcgtcgc tcaccacgac gcgttcgacg tcggctttca
     1021 tgccgacctt ggcctcggac ttcgcgcgcc gcacgtcgcg aaggacgttc tcgacgaccg
     1081 cgaggacggg aagttcggtg gcggctgcct cgacatcgag ttcggcgacg gtcggccacg
     1141 gggcgccgtg caccgaaccc tgcatccacc acgaccacgc ctcctcggtg gcgaatggga
     1201 ggaacggggc gaacagccgt tgcaacacgc tgacggctcg cgccaacgcg ttcgccgccg
     1261 aggcggatgc g
//
LOCUS       DAOOMA010000672         1262 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_144988_length_1262_cov_2.909693, whole genome shotgun
            sequence.
ACCESSION   DAOOMA010000672 DAOOMA010000000
VERSION     DAOOMA010000672.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 1262)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1262)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1262
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_144988_length_1262_cov_2.909693"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..575)
                     /locus_tag="PLP95_13770"
     CDS             complement(<1..575)
                     /locus_tag="PLP95_13770"
                     /inference="COORDINATES: protein motif:HMM:NF015431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HPB46916.1"
                     /translation="MDQRQLRALLAVGEHRSFSAAAKALNTVQSNVSTHVARLERELG
                     VTLIDRAVNELTAEGRVVAERARRIDHEYEALASDVAAMRDVVTGSVRIGIIGTTARW
                     IVPGFLTRLKAHYPQVRVVILDATTSSLVLHLAGGQLDLAIVNTPVEDPELSSEDLFV
                     EQRILVVPLEHPLADRDSVTLAEVAKHEVLIS"
     gene            872..>1262
                     /locus_tag="PLP95_13775"
     CDS             872..>1262
                     /locus_tag="PLP95_13775"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HPB46917.1"
                     /translation="MADSASASRPAKKRPPRRARTPKFSSAAIFDLDRTLLAGASGPV
                     FSDALRRHGVINSPKLPIEPALFKMFDLFGENRPTMLLTKQGVRLAKGWNRAAVEAAA
                     LDATDELMSKILPYAITEIAQHRAAGRA"
BASE COUNT          240 a          398 c          404 g          220 t
ORIGIN      
        1 gagatcaaca cctcgtgttt ggcgacctcg gcgagggtga cgctgtcgcg gtccgcgagc
       61 ggatgttcga gtggcaccac cagaatgcgt tgttcgacaa agaggtcctc ggagctgagt
      121 tcggggtctt cgaccggggt gttcacgatg gcgaggtcga gttgcccgcc ggcgagatgc
      181 aacaccagcg aggaggtggt cgcatcgaga atgacgacgc gaacctgggg gtagtgcgct
      241 ttcagccggg tcagaaaccc gggcacgatc catcgggccg tcgttccgat gattccgatg
      301 cgcacggatc cggtcaccac gtctcgcatc gccgcgacgt cggaggcgag cgcctcgtat
      361 tcgtggtcga tgcggcgggc gcgttcggcc acgacccgac cctcggcggt gagttcgttg
      421 accgcccgat cgatcagcgt cacaccgagt tctcgttcga gccgggcgac gtgggtgctc
      481 acgttggatt gaacggtgtt gagggccttc gctgcggcgg agaagctgcg gtgttccccg
      541 acagcgagca atgctcggag ctgtcgttgg tccatggtgc cgatcgtacc ccgcgatatc
      601 tctaaaagcg atcacaacta tcgacaacaa ctgctggaaa gattgcttgc agctgtttat
      661 actgaacctc acaagagcgg ccaaggcgaa cggacccccc tccgagccgg gccgccggtc
      721 tccgacagaa tcccccctct gcggaagacc aaccccgaag gaccggatcc cccctccggt
      781 ccttcggctg ttttgcccgg atgggtcggg tcctcgcata ttgcgcgaag aaccccgtgt
      841 ggaggcgggg ttcaacggat agcgtcgggg cgtggccgac tcagcatcag catcacgtcc
      901 agcgaagaag cgcccgccac gccgggcacg gactccgaag ttctccagcg cagcgatctt
      961 cgacctcgac cgaacgttgc ttgcaggagc aagcggtccg gtgttcagcg acgcccttcg
     1021 acgccacggg gtcatcaatt cgccgaaact tccgatcgag ccggcgctgt tcaaaatgtt
     1081 cgacctgttc ggcgagaacc gcccgacgat gctgctgacc aaacagggag tgcgcctcgc
     1141 caagggatgg aaccgcgccg cggttgaagc ggcagcactc gatgcgaccg acgagttgat
     1201 gtcgaagatc ctgccctacg ccatcaccga aatcgcccaa caccgagccg ccggccgagc
     1261 ga
//
LOCUS       DAOOMA010000673         1258 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_145657_length_1258_cov_3.722361, whole genome shotgun
            sequence.
ACCESSION   DAOOMA010000673 DAOOMA010000000
VERSION     DAOOMA010000673.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 1258)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1258)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1258
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_145657_length_1258_cov_3.722361"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(135..938)
                     /locus_tag="PLP95_13780"
     CDS             complement(135..938)
                     /locus_tag="PLP95_13780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020378173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HPB46918.1"
                     /translation="MLIATWNVNSLNARMPRVEEWISTVQPDVLCLQETKMSDAQFPH
                     MAFSALGYESVHHGFGRWNGVAILSKVGIDDVVSGFDDGRDDPDPDSRIIWATCGGVR
                     VASVYVPNGRELDHDHYRYKLDWLGRLREHLDRHHTPDELLVVLGDWNICPTSLDVWD
                     ESRFAGMTHVSQLERHALENVVEWGLVDTFRQRYGDAGDLFSYWDYRNGDFHKRRGLR
                     IDYLMSSTALAQLCRADLVDRNARKGAKPSDHAPVLGLFDVVVSTGAPR"
     gene            1055..>1258
                     /locus_tag="PLP95_13785"
     CDS             1055..>1258
                     /locus_tag="PLP95_13785"
                     /inference="COORDINATES: protein motif:HMM:NF027404.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB46919.1"
                     /translation="MSDDQNSTRPLGPNTWLVDEMYEQYLANPTSVSATWQEFFADYR
                     PPGAAPSPVVSHDTTDTAPSPTAP"
BASE COUNT          227 a          419 c          392 g          220 t
ORIGIN      
        1 gatcatctct cggcctaccg cggcgaggcg acggatcgcg actcggcgct gcgtctcggg
       61 cagtccgtgc agaccgtcga cccgagggct gtgggccacc tcggtcgagc catcggcgtt
      121 gggctgtgac accgtcatcg tggggccccc gtcgaaacta cgacgtcgaa caaccccaga
      181 accggcgcat ggtcggaagg cttggcgccc ttgcgggcat tgcgatcgac gaggtcggcc
      241 cgacacaact gcgccaacgc ggtgctcgac atcagatagt cgatacgcag tccacgccgt
      301 ttgtgaaagt ccccgttgcg gtagtcccag tagctgaaca ggtcgccggc gtcgccgtag
      361 cgctgacgga acgtgtcgac gagtccccac tcgacgacgt tttcgagggc gtggcgctcg
      421 agttggctga cgtgggtcat cccggcgaag cgcgattcgt cccacacgtc gagcgaggtc
      481 gggcagatgt tccagtcgcc gagcaccacc aacaactcgt cgggggtgtg atgtcgatcg
      541 aggtgttcgc gcaggcgtcc gagccaatcg agtttgtagc ggtagtgatc gtggtcgagc
      601 tctcggccat tgggaacata gaccgacgcc acccggacac cgccacaggt cgcccagatg
      661 attcgtgaat ccgggtcggg atcgtcgcga ccgtcgtcga atccgctcac cacgtcatcg
      721 atgccgacct tggagaggat cgccaccccg ttccaccgcc cgaatccgtg gtgcacggac
      781 tcgtacccga gcgcactgaa tgccatgtgt gggaactgtg cgtcggacat cttcgtttcc
      841 tgaaggcaca acacatcggg ttgcaccgtg gagatccact cctcgacacg gggcatccgg
      901 gcgttcagcg agttcacatt ccacgtggca atcaacacgc cgcgaacctt acccgttgga
      961 gtttttcgcc ccgcatttcc cggccggcgc gacgtgacaa acacccacgg agccaccacc
     1021 gcccggggga accgaacggt taggctcctc gttgatgtct gacgatcaaa attcgacgcg
     1081 tccgttgggt ccgaacacct ggttggtcga cgagatgtac gagcagtacc tcgccaaccc
     1141 cacctccgtg agcgctacgt ggcaggagtt cttcgccgac taccggccgc cgggcgcggc
     1201 gccgtcgccg gtcgtgagcc atgacaccac cgacaccgcg ccgtcgccga ccgcgccg
//
LOCUS       DAOOMA010000674         1256 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_146115_length_1256_cov_2.734388, whole genome shotgun
            sequence.
ACCESSION   DAOOMA010000674 DAOOMA010000000
VERSION     DAOOMA010000674.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 1256)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1256)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1256
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_146115_length_1256_cov_2.734388"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(121..759)
                     /locus_tag="PLP95_13790"
     CDS             complement(121..759)
                     /locus_tag="PLP95_13790"
                     /inference="COORDINATES: protein motif:HMM:NF014305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB46920.1"
                     /translation="MLRRRRTVAPPIPQSGHVLIMLHGHGSGASQLPAVLREQADDGW
                     VRVTPNASIAVDGGLSWFDTGPRGVEPESLEASVESLRSVIASLVHDAGVDPGSIALG
                     GFSQGGAVAIATAIDRGARGEQPLGGLFVLAGFVPERVDHELDLRSVGARSVLIVHPD
                     DDEVVPPFLMEALVDEIRSARDVEEVRYETVVGGHAIGEEMLGVLTGWLASR"
     gene            871..>1256
                     /locus_tag="PLP95_13795"
     CDS             871..>1256
                     /locus_tag="PLP95_13795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB46921.1"
                     /translation="MTGSHSPDAHGLNSLFSVSSPAPRRAEATEHADEIAEIVDITPH
                     LAAAPPTAGPPPLPPQFAGSSPSTSQPPTTQPPTGLPPTPQPPTTGPVVLAEPPFPDA
                     PLLTEPCAALEFAPPPAGLPAVPVTL"
BASE COUNT          210 a          488 c          368 g          190 t
ORIGIN      
        1 aggtcgtctt ggcaaccgac gcgcccgccg ccctgcccgc cagctacgcc gacgcggtcc
       61 tcaccaacac cttcagccag ttggcgacgc tgtgcgaggc acaagcggtg atcgccgcgt
      121 tcaacgagac gccaaccacc ccgtgagcac ccccaacatc tcctcgccga tcgcgtggcc
      181 cccaaccacc gtttcgtaac gaacctcctc gacgtcgcga gccgaccgga tctcatcgac
      241 gagggcttcc atgagaaacg gcggaaccac ctcgtcatcg tcggggtgca cgatcaacac
      301 cgacctcgcc cccaccgaac gcaggtcgag ttcatggtcg acccgctccg gcacgaatcc
      361 tgccaacacg aacaatcccc cgaggggttg ctccccgcgc gctccccgat cgatcgccgt
      421 cgcgatggcc accgcgccgc cttgggagaa cccaccgagc gcgatcgagc ccgggtcgac
      481 cccggcgtcg tgcaccagcg aagcgatcac cgatcgcaac gattcgaccg aggcctccag
      541 cgactcgggt tcaaccccgc ggggtccggt gtcgaaccac gacaacccgc cgtcgacggc
      601 gatcgacgca ttcggcgtca ctcgaaccca accatcatcg gcctgttcac gaagcacggc
      661 gggcagctgc gaggcgcccg atccgtgtcc gtgcaacatg atcagtacgt gtccggactg
      721 aggaatcggc ggtgccaccg tgcgacgtct cctgagcatt gggcgccccg atggggcgtt
      781 cgacctgttg accaagaaca taggttcatt acgctgagct ttgagccact cggaggcttc
      841 gttgctctcg tcgaccgttg gaagggtgtc gtgaccggtt ctcatagccc cgacgcccac
      901 gggctgaact cgctgttttc ggtgtcgtca ccggcaccgc ggcgcgccga ggcgaccgaa
      961 cacgctgacg agatcgccga gatcgtcgac atcacgccgc acctcgccgc cgctcccccg
     1021 acggcgggcc cgccgccgct tccaccgcag tttgcgggct cctctccgtc gacgtctcag
     1081 ccgccgacga cccagccgcc gacggggttg ccgccgacgc cccagccgcc gacgaccggg
     1141 ccggtcgtcc tggccgaacc gccattcccc gacgctccgc tgttgaccga accgtgcgcg
     1201 gcgctcgagt tcgctccccc gcccgctggg ttgccggcgg tgcccgtaac gctcga
//
LOCUS       DAOOMA010000675         1254 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_146453_length_1254_cov_3.154295, whole genome shotgun
            sequence.
ACCESSION   DAOOMA010000675 DAOOMA010000000
VERSION     DAOOMA010000675.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 1254)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1254)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1254
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_146453_length_1254_cov_3.154295"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..321
                     /locus_tag="PLP95_13800"
     CDS             <1..321
                     /locus_tag="PLP95_13800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015797999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SufD family Fe-S cluster assembly protein"
                     /protein_id="HPB46922.1"
                     /translation="VHVDEGAYGCKAHVQCDALILDDLSISDTYPYMEIGAQDAVIGH
                     EATVSKVADEQLFYLQSRGLSEEQAMSMVVNGFIEPITRTLPMEYAVEWSRLIELQME
                     GSVG"
     gene            complement(360..>1254)
                     /locus_tag="PLP95_13805"
     CDS             complement(360..>1254)
                     /locus_tag="PLP95_13805"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sensor domain-containing diguanylate cyclase"
                     /protein_id="HPB46923.1"
                     /translation="LGLIDLPVAVTDAETTILWCSHALADLAGYRAEILIGQRFAQAL
                     GTERPERVTAVLKDVATSERTVGRAAIAATDWAGTVRQLAISASRTRDGGDSGGLFIV
                     AENRTSERRRLYAQGANPMSTPTTGAGGVLDSLPSRFTFENQFDTSQRRAQRTRVPLA
                     VFMCGIDGYDDVLQQLGTDAAKTLIDATAARLSHALRSEDLLMYDGEDRFAIVAESVP
                     NDQVAHQTARRIRLAVSEPISIAGTELHPGLSVGITYGLGTERYDDVAEQAARALAHA
                     RSTGANQTATFDSLGLLEAQP"
BASE COUNT          200 a          389 c          443 g          222 t
ORIGIN      
        1 gtgcacgtcg acgagggggc gtacggctgc aaggcccatg tgcagtgcga cgccctgatc
       61 ctcgacgacc tgtcgatctc cgacacctac ccgtacatgg agatcggagc ccaggacgcc
      121 gtcatcggcc acgaggcgac ggtgtcgaag gtcgccgatg aacagctgtt ctacctgcag
      181 agccggggcc tgtccgagga gcaggcgatg tcgatggtcg tcaacggatt catcgaaccg
      241 atcacccgga cgttgccgat ggagtacgcc gttgaatgga gccgtctgat cgaactccag
      301 atggagggca gcgtcggctg accgcgcctg tggctggcgt tgacggtcga cgcgagcgct
      361 cagggttgcg cttcgagtag accaagcgag tcgaacgtgg ctgtctggtt ggctccggtc
      421 gaacgggcgt gggcaagcgc ccgcgcggcc tgttcggcga cgtcgtcgta gcgctcggtg
      481 cccaacccgt aggtgattcc caccgagagt ccagggtgga gttcggttcc agcgatcgag
      541 atcggttcgc tcaccgcgag gcggatgcgc cgtgccgtct ggtgggcgac ttggtcgttg
      601 ggcaccgatt cggccacgat ggcgaagcgg tcctcgccgt cgtacatcag caggtcttcg
      661 ctgcgtagcg cgtgcgacag tcgtgccgca gtcgcgtcga tcaacgtctt ggctgcgtcc
      721 gttccgagtt gctgcaggac gtcgtcgtag ccgtcgatcc cgcacatgaa caccgcgagg
      781 ggcacccggg tgcgttgggc acggcgctgg gatgtgtcga actggttttc gaaggtgaac
      841 cgggagggca gcgagtcgag cactccgcca gcaccggtcg tcggggtcga catggggttc
      901 gcgccctgag cgtaaaggcg gcgtcgctcc gatgtgcggt tctccgcgac gatgaacaac
      961 ccgcccgaat cgcccccgtc gcgcgtgcgt gacgccgaaa ttgcaagctg gcgtaccgta
     1021 ccggcccagt cggtcgcggc gatcgcggcc cggccgaccg tccgctcgct cgtggcgacg
     1081 tctttgagga cggcggtcac gcgttccggt cgctcggtgc caagtgcttg agcgaaacgc
     1141 tgaccgatca gtatctccgc ccggtacccg gcgaggtccg cgagcgcatg gctgcaccac
     1201 aagatggtgg tctcggcatc ggtgacggcc accggaaggt cgatcagtcc gagc
//
LOCUS       DAOOMA010000676         1254 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_146472_length_1254_cov_2.955796, whole genome shotgun
            sequence.
ACCESSION   DAOOMA010000676 DAOOMA010000000
VERSION     DAOOMA010000676.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 1254)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1254)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1254
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_146472_length_1254_cov_2.955796"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            116..643
                     /locus_tag="PLP95_13810"
     CDS             116..643
                     /locus_tag="PLP95_13810"
                     /inference="COORDINATES: protein motif:HMM:NF015648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HPB46924.1"
                     /translation="MQPPTSSRRTLPPPAIHLYRLHNAPWVLLAVVMVVFGVIAFVAD
                     EAVLGAISMTAAVVAAAVWWVWSTLRFRAVSWELTEGTVESRLGVVRRVESVLPRGRV
                     QNVTKSSGPVERALGMATIHVHSAGANTPDIVIPHVYEADAEHLRSVLLPREQTEGSA
                     PSAVPESQPDVDADV"
     gene            630..1037
                     /locus_tag="PLP95_13815"
     CDS             630..1037
                     /locus_tag="PLP95_13815"
                     /inference="COORDINATES: protein motif:HMM:NF015648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HPB46925.1"
                     /translation="MPTSETSSHLDDPIAAWRHWTRVDPRSTLPIAGPVVVVLIVLWL
                     QQFAPTSGTAGDVAEAFVEDWTGLVLAAAFAAGAAARYLSTRFRIADGVVQWRTGILT
                     HQAKELGLDRIQDVTITRPLVARGQSAGHIGSA"
     gene            1057..>1254
                     /locus_tag="PLP95_13820"
     CDS             1057..>1254
                     /locus_tag="PLP95_13820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB46926.1"
                     /translation="MPESESLWQDDFRAIPAHAAANALRVKIEVPESHLFFISKFSGF
                     SRKFEAEAVAIGGAIDEVEAAP"
BASE COUNT          205 a          381 c          427 g          241 t
ORIGIN      
        1 tctccgataa ccagtggtcg tgatcacgac cactggttat cggagaacgg gctggggcgg
       61 gagaacaggc tggggcggga gaacaggctg gggcgggatc ccgtagcctg cagggatgca
      121 gcctccgacg tcgtcgcgcc ggaccctccc accaccggcg attcatctgt accgtctcca
      181 caacgcgccc tgggttctgc tggcggtcgt gatggtggtg ttcggggtga tcgcgttcgt
      241 tgccgacgag gcggtcctcg gggcgatctc tatgacggca gcggtcgttg cggcggccgt
      301 gtggtgggta tggtcgacgc tgcgattccg agcggtgtcg tgggagctga ccgagggcac
      361 cgtcgagtcg cggctcggcg tggttcgacg ggtcgaatcg gtgctcccac gcggcagggt
      421 tcagaacgtg accaagtcgt cgggaccggt cgagcgggcg ctcgggatgg ccacgatcca
      481 tgttcattcc gccggagcga acacccccga catcgtgatt ccccacgtgt acgaagccga
      541 tgccgaacac cttcgttcgg tgttgctgcc acgagaacag acggagggtt cagctccctc
      601 agcggtgccg gagtcccagc ccgatgtcga tgccgacgtc tgagacgagt tcgcacctcg
      661 acgatccgat cgcggcgtgg agacactgga cccgagtcga tccgcgttcg acgctgccga
      721 tcgccggccc ggtcgtggtg gtgttgatcg tgttgtggct ccagcagttc gccccgacct
      781 ccggcacggc aggcgatgtc gccgaagcat tcgtcgagga ctggacgggg ctcgtcctcg
      841 ctgcagcgtt cgccgcaggt gctgccgctc ggtacctgtc gacccggttt cgaatcgccg
      901 acggcgtcgt gcaatggcgc accggcatcc tcacacacca ggcgaaggaa ctcggccttg
      961 accgaatcca ggatgtgacg atcacgcggc cgctggtggc ccgagggcaa tccgctgggc
     1021 acatcggatc agcctgaatt tgaacccatt gatcgcatgc cggaatccga gtcgctttgg
     1081 caggatgact tccgcgctat tcccgcgcat gccgcggcca atgcattgcg tgtcaaaatc
     1141 gaggtgccgg aatctcatct tttcttcatt tccaaattct ccggttttag ccgaaaattt
     1201 gaggcggagg cggtcgcaat cgggggcgcg attgacgagg tggaagcggc gccc
//
LOCUS       DAOOMA010000677         1234 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_150112_length_1234_cov_4.212892, whole genome shotgun
            sequence.
ACCESSION   DAOOMA010000677 DAOOMA010000000
VERSION     DAOOMA010000677.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 1234)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1234)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1234
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_150112_length_1234_cov_4.212892"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..614)
                     /locus_tag="PLP95_13825"
     CDS             complement(<1..614)
                     /locus_tag="PLP95_13825"
                     /inference="COORDINATES: protein motif:HMM:NF012350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HPB46927.1"
                     /translation="MSEQLGTDRSEGLSSAEVAHRLATFGPNRLAEKPSTPLWRRFID
                     QFKSVLILLLAIGAVAAAAVGDVKDAIVIASVLVINAILGTVQEARAENSLDALKAML
                     SATARVRRDGSLHDIDATELVPGDVISLEAGDRVPADARILASYDGEADESSLTGESM
                     AVDKREAAIAKAEVPLGDRNNMIWMNTTMTRGRVEAVVTATAMDT"
     gene            1007..>1234
                     /locus_tag="PLP95_13830"
     CDS             1007..>1234
                     /locus_tag="PLP95_13830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB46928.1"
                     /translation="MQSRSLHFAESARALSATARGLGLDVPAIRSRPRRADLDRSLRR
                     RSDGQVVVSVRLDERPFAAVQADLVEAFVVVN"
BASE COUNT          178 a          384 c          436 g          236 t
ORIGIN      
        1 gtgtccatgg ccgttgcggt gacgaccgcc tcgacccgcc cccgagtcat cgtggtgttc
       61 atccagatca tgttgttgcg gtcgccgagc gggacctctg ccttcgcgat ggcggcctcg
      121 cgcttgtcga ccgccatcga ctcgccggtc aatgaggact cgtcggcttc gccgtcgtag
      181 ctcgccagga tgcgggcgtc ggcagggacc cggtcgccgg cttcgagcga gatcacgtct
      241 cccgggacca attcggtggc gtcgatatcg tggagggacc cgtcgcgtcg cacccgagcg
      301 gttgctgaca acatcgcctt gagagcgtcg agggagttct ccgcccgcgc ttcctgcacg
      361 gtgccgagga tggcgttgat gaccagtacc gacgcgatga cgatggcgtc tttcacatcg
      421 ccgacagccg ctgcggccac cgctccgatc gcgagcagca ggatcagtac ggacttgaac
      481 tgatcgataa agcgtcgcca caggggcgtc gacggctttt ccgccagacg attgggtccg
      541 aaggtggcaa ggcggtgggc cacctctgcg cttgagagcc cttcggagcg gtcggtgccg
      601 agttgctcac tcacgacctc cgattcgagc gtccaagctg gagcatcggt tgtgggggat
      661 ggccggccat ctggtcgtgt ttgggcggga gagaccgggt tcatgggcgc gtaggctcct
      721 tcgggtcgtt ggacggaacg aaccgaaggt cttccccacc tcgcatcggc caccggtcga
      781 acggggtggc cggcaccggg accagcgacg catcggcgtc ggtggtgccg tgatgacgat
      841 gccggcactt gcggggtact ccccttcgtg ttgttgaggg cgttgaggcg ccgcgagcac
      901 tgtagcgcga gcagcgggtc gcctcgggtg ctgggcctga gacctccggc actgtcggtt
      961 acggccaccc ggatcgattg tcggtggtcg cggagatgat gtgatcatgc agtcccgctc
     1021 cctccatttc gcagaatcgg ctcgtgcact gtccgcaacc gcacgtggtc tggggctcga
     1081 cgtgccggcc atcaggagcc ggccacggcg agcagacctc gaccggtcgt tgcgccgccg
     1141 aagcgatggg caggtcgtgg tgtcggttcg gctcgacgag cgtccgttcg ctgcggtgca
     1201 ggccgatctc gtggaggcgt tcgtggtcgt caac
//
LOCUS       DAOOMA010000678         1213 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_154248_length_1213_cov_4.306563, whole genome shotgun
            sequence.
ACCESSION   DAOOMA010000678 DAOOMA010000000
VERSION     DAOOMA010000678.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 1213)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1213)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1213
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_154248_length_1213_cov_4.306563"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1124)
                     /locus_tag="PLP95_13835"
     CDS             complement(<1..1124)
                     /locus_tag="PLP95_13835"
                     /inference="COORDINATES: protein motif:HMM:NF014998.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004144 - diacylglycerol O-acyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0045017 - glycerolipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="wax ester/triacylglycerol synthase family
                     O-acyltransferase"
                     /protein_id="HPB46929.1"
                     /translation="MSDRRSDTTGHEATIALGHHDRMNDTEALMWSLEKDPSLRSTIT
                     VVFLLDGTIDRDVLTRRVERMTRTVPRLRQRVRSNTLSVAPPRWETDPNFDLSFHLWW
                     VRAPGSATMRDVLSIAEPVAMSGFDRARPLWRAIVIEGLPNGRSAIVMKVHHSIADGI
                     TAVQLQLELFDFEPDADERELPPIPQAHVLSQPERFADAIEHQTRHQLAVFGRIAGTL
                     LDNAQHALTDPATALRDGAALAASLARVARPGTHTMSPLMTERSLSLRFDTLTVDLEA
                     LRSAGRAAGGTLNDAFVAALARGMYRYHLAHDVDCDELRLAIPISMHNADGGVGSGAG
                     DGGGNAFIPARVEIPIDLPDPVELMSTIHDIVDGARHEPA"
BASE COUNT          195 a          384 c          423 g          211 t
ORIGIN      
        1 ttcgccggtt cgtggcgcgc cccatcgacg atgtcgtgga tggtcgacat caactccaca
       61 ggatcgggga ggtcgatggg gatctcgaca cgggccggaa taaacgcgtt cccgccccca
      121 tcaccagccc cgctaccaac cccgccgtcg gcgttgtgca tgctgatcgg aatcgcgagt
      181 cgcaactcgt cgcagtcgac gtcgtgcgcg aggtggtagc gatacatgcc ccgcgccaac
      241 gccgcgacga acgcgtcatt gagcgtccct ccggccgccc gaccggcgct gcgcaacgct
      301 tcaaggtcca ccgtgagggt gtcgaagcgc agcgacaggg aacgttcggt catcagcggg
      361 ctcatggtgt gggtgcccgg tcgcgccacc cgtgcgagcg aggccgccag tgcggctccg
      421 tcgcgcagcg ccgtggcggg gtcggtgagc gcatgttgtg cattgtcgag cagggtgccg
      481 gcgatacgtc cgaaaacggc cagttggtgt cgcgtctggt gttcgatggc gtcggcgaat
      541 cgctcgggct ggctgagcac gtgtgcctgc gggatcggcg gaagctcgcg ttcgtcggcg
      601 tcgggttcga aatcgaacaa ctcgagttgc agctgtacgg cggtgatccc atccgcgatc
      661 gagtggtgca ccttcatcac gatggcggat ctcccgttgg ggaggccctc gatcacgatc
      721 gcccgccaca gcggtcgggc acggtcgaac ccggacatcg ccaccggctc ggcgatgctc
      781 aacacatcgc gcatcgttgc ggaaccgggt gcccgaaccc accacaggtg aaaggaaaga
      841 tcgaagttgg ggtcggtctc ccagcgggga ggcgcaaccg acaacgtgtt ggaccgcacc
      901 cgttggcgca gccggggtac ggtcctggtc atccgctcga cccggcgggt caggacgtcg
      961 cgatcgatgg tgccgtcgag gaggaacacc acggtgatgg tggagcgaag cgacggatcc
     1021 ttctccaggg accacatgag cgcttcggtg tcgttcatcc gatcgtgatg gcccagagcg
     1081 atggtcgctt cgtgaccggt ggtgtcgctt cggcggtcac tcatggctgt tccttcccgg
     1141 cgtcggtgct cgccgcttcg ctcgaaactg ggatccgcca cggggtgccg gcatcggcga
     1201 tcccggaggc gac
//
LOCUS       DAOOMA010000679         1207 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_155530_length_1207_cov_3.585938, whole genome shotgun
            sequence.
ACCESSION   DAOOMA010000679 DAOOMA010000000
VERSION     DAOOMA010000679.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 1207)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1207)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1207
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_155530_length_1207_cov_3.585938"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..601
                     /locus_tag="PLP95_13840"
     CDS             <1..601
                     /locus_tag="PLP95_13840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB46930.1"
                     /translation="PSAQTATLAGTVPAAKPDSTEAGVDEGARETGDGEHEAAAWLAA
                     LQESPASPRGRRDPRTERAEEQAAVAIGRGLTFGRGEVLELAELDDATLSMMEEYGFV
                     SVNVIAGESTYDAAALAVVKSLKRFLAKGVEPRHLRMFKNAAERESGFYEQMVLPLLK
                     QRNPRAIEQATALLNELAGAGEQLHRALVHQALGRYRPD"
     gene            682..>1207
                     /locus_tag="PLP95_13845"
     CDS             682..>1207
                     /locus_tag="PLP95_13845"
                     /inference="COORDINATES: protein motif:HMM:NF014618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nuclease family protein"
                     /protein_id="HPB46931.1"
                     /translation="MIEVELVGVTMDNTANTPVVVLRELSNPPDVDSGSDASPEPDRR
                     RSISIFIGAPEATAIAFALEHINPPRPLTHDLMVDLLVKLGIGVTRIVITEIHDATFY
                     AELHLSHGGSEFVISCRPSDALALAARVCCGLFVTEAVIDEAGVLPMSEEDLATDPGD
                     PDEIVEEFRQFIDSI"
BASE COUNT          209 a          374 c          417 g          207 t
ORIGIN      
        1 cccctcggcg cagacggcga cactcgctgg caccgtgccc gcagcgaagc ccgattcgac
       61 cgaagccggt gtcgacgagg gtgcccggga aaccggcgac ggcgagcacg aggccgccgc
      121 ctggctggcg gccctgcagg agtcgccggc gtcgccgcgt ggccggcgcg atccgcgcac
      181 ggaacgggcc gaggagcagg cggcggttgc catcggtcgc gggctcacct tcgggcgagg
      241 cgaggttctg gaactcgccg agctcgacga tgcgacgctg tcgatgatgg aagagtacgg
      301 attcgtctcg gtgaacgtca tcgccggcga gtcgacctac gacgccgccg cattggcggt
      361 ggtgaagtcg ttgaaacgct tcctggccaa gggggtcgaa cctcgccacc tgcggatgtt
      421 caagaacgcc gccgaacgcg agtcgggatt ctacgaacag atggtgctgc cgttgctcaa
      481 acagcgaaac ccgcgggcga tcgaacaggc gacggcgctg ttgaacgagt tggccggggc
      541 cggcgagcag ctgcaccggg cgctggtcca tcaggcactt gggcggtatc gcccggactg
      601 atcggtgtcg cccatcgccg gatggctcac aaacgcgtgg tggcgactcg tgagcggttt
      661 gggcgggact aacgtttggt catgattgaa gtggagttgg tcggcgtgac catggacaac
      721 accgcgaata cgccggtggt cgtgttgcgc gagttgtcca accccccgga cgtggactcc
      781 ggttcggacg cctcacccga accggatcga cggcgatcga tctccatctt catcggtgcg
      841 cctgaggcga ccgcaattgc gtttgcgctg gagcacatca acccgccgcg cccgctcacc
      901 cacgacctga tggtagacct gctcgtgaaa ctcggaatcg gcgtaacccg gatcgtcatc
      961 accgagattc acgatgccac gttctatgcg gaactccact tgagccacgg cggttcggag
     1021 ttcgtgatct cctgtcggcc ctccgatgcg ctggccctgg cggcacgggt gtgctgcgga
     1081 ttgttcgtca ccgaagcggt gatcgatgaa gcaggggtgc tgcccatgtc ggaggaggat
     1141 ctggcgacgg atcccggcga cccggacgag atcgttgagg agttccgcca gttcatcgat
     1201 tccatca
//
LOCUS       DAOOMA010000680         1207 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_155536_length_1207_cov_3.497396, whole genome shotgun
            sequence.
ACCESSION   DAOOMA010000680 DAOOMA010000000
VERSION     DAOOMA010000680.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 1207)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1207)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1207
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_155536_length_1207_cov_3.497396"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            31..>1207
                     /locus_tag="PLP95_13850"
     CDS             31..>1207
                     /locus_tag="PLP95_13850"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPB46932.1"
                     /translation="MGIAMRAVVDDRSLVRLNGARPGRISMFAWALGSGLAALSGILS
                     APLSQFDSITLTYTVVNCYAAAVFGKLKSLPMTFLGALILGLAESYYAGFVVTKTPEI
                     GGFSTGGFQFALPAIMMMIVMVAQPQARLRTGGIQRTRAAMRVPTLNMSLVGAGALIV
                     AMYGITTLIPSDPARSPLVWTMYFALVSLSLVPLTGFAGSISLGQMTFVGLGAVVMSK
                     VAAQTASLGALIVALVVCAVVGAIIALPALRLTGIYLALSTVSFSLVMSKVFFSQRKL
                     ANGNTVRVEPIDLGFWKPVTNQDLVMLGTVVFALLGVGVCALRRSSYGRRLIAMKDSP
                     VACATLGLDLTRTKIGVFAFSASIAGLAGALSARSVPVSEYDLLNSMSVSMLAVVGGI
                     "
BASE COUNT          163 a          397 c          391 g          256 t
ORIGIN      
        1 ggactccgct tggtcctgta caagtcgcgc atgggtatcg ccatgcgagc ggtcgtcgat
       61 gaccgctcgc tggtgcgtct caacggcgcc cggcccggcc gtatctcgat gttcgcctgg
      121 gcgctcggtt ccggcctcgc cgcgctgtcg ggaatcctca gcgctccgct gagccagttc
      181 gactcgatca ccctcaccta cacggtcgtg aactgttacg ccgcggcggt tttcggcaag
      241 ctcaagagcc tgccgatgac ctttctcggc gcactcatcc tcgggctggc cgagtcgtat
      301 tacgccggtt tcgtcgtgac aaagaccccg gagatcggcg gattctcgac cggcggattc
      361 cagttcgcac ttccggcgat catgatgatg atcgtcatgg tcgcccagcc gcaggcccgc
      421 cttcgcaccg gcggcattca acggacccgg gcggcgatgc gggtgccgac gctgaacatg
      481 tcgctcgtgg gggcgggggc gttgatcgtc gccatgtacg gcatcaccac cctcattccc
      541 tccgacccgg cgcgttcacc gctggtgtgg accatgtact tcgcgctcgt cagcctgtcg
      601 ctggttccac tcaccggatt cgcggggtcg atcagcctgg ggcagatgac gtttgtcggt
      661 ctcggagcgg tcgtgatgag caaggtggcg gcccagacgg catcgctcgg ggcgctcatc
      721 gtcgccctcg tcgtgtgtgc ggtcgtcggg gcgatcatcg ccctgcccgc ccttcgcctc
      781 accggcatct acctcgcgct ttccacggtg tcgttctcgc tggtgatgtc gaaggtgttc
      841 ttcagtcagc gaaagctcgc gaacggcaac accgtgcgcg tcgagccgat cgatctcggg
      901 ttctggaagc cggtcaccaa ccaggacctc gtgatgttgg gaaccgtggt gttcgcgttg
      961 ctcggcgtcg gagtgtgtgc cttgcgtcgc agttcctacg gacggcgcct catcgccatg
     1021 aaagacagcc cggttgcgtg cgccaccctg ggactcgacc tgacgcggac caagatcggc
     1081 gtgttcgcct tttcggcgtc gatcgccggg ctcgcaggcg cgttgtcggc ccgctcggtt
     1141 ccggtcagcg aatacgacct gctcaactcg atgtcggtgt cgatgttggc ggtggtcggc
     1201 ggcatcg
//
LOCUS       DAOOMA010000681         1197 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_157580_length_1197_cov_4.258319, whole genome shotgun
            sequence.
ACCESSION   DAOOMA010000681 DAOOMA010000000
VERSION     DAOOMA010000681.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 1197)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1197)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1197
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_157580_length_1197_cov_4.258319"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            193..1014
                     /locus_tag="PLP95_13855"
     CDS             193..1014
                     /locus_tag="PLP95_13855"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HPB46933.1"
                     /translation="MWGEVRTIEALAAIFSFKNWQDIHYASNAETGMRTLGPLSPFWS
                     LSIEEQFYLGMTIVIGLAMFSVHWRRWLAAAFVVVGAYSVWSMITIESTLNREFFGTD
                     TRAAELVVGCLLALAIDRWGWPSSPRWSWVGWASLLGTLWFWSTVTEHDRWVLRGGLS
                     AMSLLTVGLILGAAPGTGWFATAFSWPPLVELGKMSYAVYVSHWPVALALRPDNVGFG
                     GWPLLGIRILASFAVAFALTRWIETPIRTRARLQGAQGPIAFVIVLTAIVVLTRL"
BASE COUNT          164 a          378 c          416 g          239 t
ORIGIN      
        1 cgggttcgtt catcgggccg acggcgttct tcacgttgtc gggcttcttg atcacctcgc
       61 tgttgatggt cgaaaaggac aatgacgaac gcgtcgactg gctcgggttc tggcgtcggc
      121 ggttccgccg cttgttgccg gcctcgatga cggtggtcct ggtggcggtc gcggctccga
      181 aggtcgtgga gcgtgtgggg ggaggtgcgc acgatcgagg ccctagcggc gatcttcagc
      241 ttcaagaact ggcaggacat ccattacgcc tcgaatgccg aaactggtat gcgcacgctc
      301 gggccgctgt caccgttctg gtcgctgtcg atcgaagagc agttctacct ggggatgacg
      361 atcgtcatcg gattggcgat gttcagtgtc cactggcgcc gctggctggc cgcggcgttc
      421 gtggtggtcg gggcgtactc ggtgtggtcg atgatcacga tcgagtcgac cttgaaccgc
      481 gagttcttcg gcaccgacac ccgcgccgct gagctggtgg tcggctgcct gttggcgttg
      541 gcgatcgacc gctggggttg gccgagttca ccccgatgga gttgggtcgg ctgggcgtcg
      601 ctcctcggca cgctgtggtt ctggtcgacc gtgaccgaac acgaccgttg ggtgttgcgc
      661 ggcgggctgt cggcgatgtc gctgctgacc gtcggcctga ttctcggcgc ggcaccgggt
      721 acgggttggt tcgccacagc gttctcgtgg cccccgttgg tcgagctcgg caagatgagt
      781 tacgcggtct acgtcagcca ctggcccgtc gccctcgcac tgcgcccgga caacgtcgga
      841 ttcggcggct ggccgctgct gggcatccgg atcctcgcat ccttcgccgt ggcgtttgcg
      901 ctcacgcgct ggatcgaaac cccgattcgc acccgcgcca ggctgcaagg ggcacagggt
      961 ccgatcgcat tcgttatcgt cctcacggcg atcgtcgtcc tcaccagact ctgaccgaca
     1021 ccgacaccgg ctccgggaac gcgacggcgc aagagcggcg ccgcctcagc cgtcgggcgc
     1081 gacgagccac aacgtcaccg acatggcccc gaggcgggta ccgagttcga aggcgcgttc
     1141 gaggtcgtcg cgatcggcgc cgtcgatcac gagccagccc tgctgccaag cgtcgcg
//
LOCUS       DAOOMA010000682         1192 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_158702_length_1192_cov_2.631486, whole genome shotgun
            sequence.
ACCESSION   DAOOMA010000682 DAOOMA010000000
VERSION     DAOOMA010000682.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 1192)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1192)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1192
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_158702_length_1192_cov_2.631486"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..368
                     /locus_tag="PLP95_13860"
     CDS             <1..368
                     /locus_tag="PLP95_13860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB46934.1"
                     /translation="TTAAPTTTAAPTTTAAPTTTTAKRVVSASVSATKARKSNGYKST
                     ATVTLSATTSPSVSGTSVIIGLYASNNSKLGEGNCTISGSTCTFNVTLASGYSGTYWK
                     IISVGSSDWDGVKPSATAM"
     gene            427..834
                     /gene="ndk"
                     /locus_tag="PLP95_13865"
     CDS             427..834
                     /gene="ndk"
                     /locus_tag="PLP95_13865"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012230372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006165 - nucleoside diphosphate
                     phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HPB46935.1"
                     /translation="MNRTFIICKPDAVERGLVGEILTRFERRDLKVVAAELRTIDRET
                     ASKHYAEHEGKPFYEDLVTFIGRSPALCAVIEGPTDTWQIVRTMMGATNPAESDPGTI
                     RGDFGLEMSENLIHGSDSAESAAREIAIFFPGL"
     gene            969..>1192
                     /locus_tag="PLP95_13870"
     CDS             969..>1192
                     /locus_tag="PLP95_13870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018225978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HPB46936.1"
                     /translation="MASRFSSFSGFMGRDMAIDLGTANTLVYVRGEGIVLREPSVVAV
                     HATTGQPVAVGAEAKRMIGRKTKAESGGFGV"
BASE COUNT          219 a          378 c          390 g          205 t
ORIGIN      
        1 cgacgacggc tgcgcccacg accacggctg cgcccacgac gacggctgcg cccaccacga
       61 cgactgcaaa gcgcgtggtg tcggcgtcag tcagcgcaac gaaggcccga aagagcaacg
      121 gctacaagtc caccgcgacg gtcacactgt cggcgacgac ttcgccgtcg gtgtcgggca
      181 cctcagtcat catcggcctg tacgcctcga acaattcgaa gctcggtgaa ggtaactgca
      241 cgatctccgg atcgacatgt accttcaacg tcacccttgc gtcggggtat tcaggcacgt
      301 actggaagat catctcggtc ggctcatcgg attgggacgg agtgaagccg tcggcgaccg
      361 cgatgtgaga ttcggtggcg cgagattcgg tggcgtcggg gaactgcgcc tagcatcttc
      421 gccgccatga atcgcacctt catcatctgc aaacccgacg ccgtcgaacg tggcctcgtc
      481 ggagagatcc tcacccgttt cgaacgccgc gacctcaagg tcgtcgccgc cgaactccgc
      541 acgatcgacc gtgagaccgc gtcgaagcat tacgccgaac acgagggcaa gccgttctac
      601 gaggacctcg tcacgttcat cgggcgttcg ccggcgctgt gtgcggtgat cgagggcccg
      661 accgacacgt ggcagatcgt gcgcacgatg atgggtgcca ccaaccccgc ggagtcggac
      721 ccagggacga tccgtggcga tttcggactg gaaatgtcgg agaacctcat tcacggttca
      781 gactccgccg agtcggcggc gcgcgagatc gccatcttct ttccgggcct gtgagcctcg
      841 gcgcccaacg ctgctgagac cgcatcaggt ctcgcgatgg aggggtccgg agtgctggag
      901 taccatggct ggcgttgtta gtcgcggatg ccgacgccgc gccggccgac cggaggagta
      961 gcgaagccat ggcatcacgg ttttcctcgt tctccggttt catgggtcgc gacatggcca
     1021 tcgatctcgg caccgcgaac acgctggtgt acgtgcgtgg cgagggaatc gtcctgcgcg
     1081 agccatcggt ggtcgccgtc cacgccacca ccggccaacc ggtggcggtc ggggcggagg
     1141 cgaagcgcat gatcgggcgt aaaacaaagg ccgagtccgg cgggtttggt gt
//
LOCUS       DAOOMA010000683         1189 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_159286_length_1189_cov_3.447090, whole genome shotgun
            sequence.
ACCESSION   DAOOMA010000683 DAOOMA010000000
VERSION     DAOOMA010000683.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 1189)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1189)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1189
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_159286_length_1189_cov_3.447090"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            5..>1189
                     /gene="rimO"
                     /locus_tag="PLP95_13875"
     CDS             5..>1189
                     /gene="rimO"
                     /locus_tag="PLP95_13875"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HPB46937.1"
                     /translation="MTQTYWVETLGCPKNQVDSDKLVGKLVADGYVAASDASQADLVV
                     VNTCAFIEEARQESIDTVLALSEDRAEHAKVVVTGCMAQRYGDELAAALPEVDTVAGF
                     GQDFIDSPGPGAPSSPHSSTHSSSRSLIPVSAAPVPEFDLLNLPRPAASAPWAYVKIA
                     EGCDRNCGFCAIPSFRGPQRSRSIDSVVAEVEQLDVREVVLVAQDLAAYGRDQGKGER
                     SIVALVDQVARSVDWVRLLYLYPSDLNEALIEAMFGARVPYFDLSLQHVSRPLLRRMR
                     RWGEGETFLERIEAIRSIDPAAAFRSNFIVGYPGETEADHDALLEFVEAAQLDWCGFF
                     AFSDEEGTYAHDLDGHIDATLMGERLAELRELQDDITAARRDALIGETITVLVDEPGV
                     ARS"
BASE COUNT          194 a          384 c          394 g          217 t
ORIGIN      
        1 caccgtgacg cagacgtact gggtcgagac gctcggctgc cccaagaacc aggttgattc
       61 cgacaaactc gtcggcaagc tggtcgcgga cggatacgtg gcggcctccg acgcgtcgca
      121 ggccgatctc gtcgtcgtca acacctgtgc gttcatcgag gaagcacgac aggagtcgat
      181 cgacaccgtg ctggcgttga gcgaggatcg tgccgagcac gccaaagtgg tcgtcaccgg
      241 gtgtatggcc cagcgctacg gcgacgagct tgcggccgcg ctgcccgagg tcgataccgt
      301 cgccgggttc ggtcaggatt tcatcgattc ccccggccct ggcgcgccct catcgcccca
      361 ctcttcgacc cattcgtcga gccgatcact catcccggtg tcggcagcac cggtgcccga
      421 gttcgacctg ttgaatctcc cgcggccggc cgcttccgca ccgtgggcat atgtgaagat
      481 cgccgaggga tgcgaccgca actgcgggtt ctgtgcgatc cccagctttc gtggaccaca
      541 gcgaagccgg tcgatcgaca gtgtcgtcgc cgaggtcgaa cagctcgatg tccgcgaggt
      601 cgtgctggtc gctcaggacc tggcggcgta cggacgtgac cagggtaagg gggagcgcag
      661 catcgtggcg ctggtcgacc aggtggctcg cagcgtcgac tgggtgcggc tgttgtatct
      721 gtacccctcc gacctcaacg aggcgctcat cgaagccatg ttcggagcgc gggtcccgta
      781 tttcgacctg tccctccaac acgtcagtcg tccgctgttg cgccgcatgc gccgctgggg
      841 tgagggtgag acgttcctcg aacgcatcga ggccatccgt tcgatcgacc cggccgcggc
      901 gttccggtcg aacttcatcg tcgggtatcc aggcgaaacc gaggccgacc acgatgcgtt
      961 gctcgagttc gtggaggccg cgcagctcga ctggtgtggg ttcttcgcgt tctccgacga
     1021 ggagggaacc tacgcccacg acctcgacgg ccacatcgac gcgaccttga tgggggagcg
     1081 gttggctgag cttcgcgaat tgcaggacga catcaccgcg gcgaggcgtg acgctctgat
     1141 cggcgagacg atcaccgttc tggtggacga acccggcgtc gcccgcagt
//
LOCUS       DAOOMA010000684         1187 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_159699_length_1187_cov_3.776502, whole genome shotgun
            sequence.
ACCESSION   DAOOMA010000684 DAOOMA010000000
VERSION     DAOOMA010000684.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 1187)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1187)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1187
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_159699_length_1187_cov_3.776502"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..314)
                     /locus_tag="PLP95_13880"
     CDS             complement(<1..314)
                     /locus_tag="PLP95_13880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold nucleic acid binding domain-containing
                     protein"
                     /protein_id="HPB46938.1"
                     /translation="MTDSVSAPASTPAPASTSASTSVARPSLTTAFRTHTCGELRAAN
                     IGETVTLSGWVARRREHGEHLAFIDLRDYTGLTQCVVDGAADLRSEYVVSVTGTVSAR
                     LEG"
     gene            complement(332..>1187)
                     /locus_tag="PLP95_13885"
     CDS             complement(332..>1187)
                     /locus_tag="PLP95_13885"
                     /inference="COORDINATES: protein motif:HMM:NF024785.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase regulatory
                     subunit"
                     /protein_id="HPB46939.1"
                     /translation="VGLWDFYRSLGLSQLRLELNSIGDPESRARYMDALRAFLNGRVG
                     DLDPEDAAKIDRHPMRVLDSKRTATRAALDGVPTLMESMSEVALAHFDRVQQGLAAAG
                     VPVTLNPRLVRGLDYYTHTVFEIISEAMDAAQSTIGGGGRYDGLVESLGGPPLPGVGF
                     GSGIERILLACDREGVFETPELTVDAFVVELGGDGSHGRDLLLELRRGGVSCDRAYGG
                     RSLRAQMKQADRSGARWGIIVGDDERAGGVVSLRDLRSSDHEGAQTTVPRGEIVDVLR
                     RRVQIDAG"
BASE COUNT          202 a          439 c          372 g          174 t
ORIGIN      
        1 ccctcgagcc gcgcgctcac ggtgccggtc acgctcacca cgtactcgct tcgcagatcc
       61 gcggcaccgt cgacgacgca ctgggtcagg cccgtgtagt cgcgcaggtc gatgaaggcg
      121 aggtgttcgc cgtgctcgcg gcgccgggcc acccagccag acagcgtgac ggtctcaccg
      181 atattcgcgg ccctcagctc cccacaggtg tgggtgcgaa acgcggtcgt caacgacggc
      241 cgagcgacac tcgtcgaggc ggaggtgctg gcgggcgcgg gggtgctggc gggcgcggaa
      301 acagagtcgg tcacgaagcg agcctaccgg ctcaaccagc gtcgatctgc acccgccggc
      361 gcagcacgtc gacgatctcg ccacgcggca cggtcgtctg tgccccctcg tggtcgctcg
      421 agcgaaggtc gcgcaacgac acgacgccgc cggcccgttc atcgtcgccg acgatgattc
      481 cccaccgcgc tcccgaacga tccgcctgtt tcatctgcgc ccgcagcgat cgcccgccgt
      541 aggcccggtc acacgacact cccccacgac ggagctccaa caacaggtcg cgcccgtggg
      601 aaccgtcgcc tccgagttcg accacgaacg cgtcaaccgt caactcgggc gtctcgaaca
      661 caccctctcg atcgcatgcc aacaggatcc gctcgattcc cgacccgaac cccacacccg
      721 gcaatggcgg gccgccgagg gattccacca gtccgtcgta acgcccgccc ccgccgatcg
      781 tcgactgggc tgcgtccatt gcctcgctga tgatctcgaa caccgtgtgg gtgtaatagt
      841 cgaggccgcg aaccaaccgc ggattgagcg tcactggaac cccggccgcg gccaaccctt
      901 gttgaacccg gtcgaaatgg gcgagcgcaa cctccgacat cgactccatc agggtcggca
      961 ccccgtcgag cgcagcgcgg gtcgccgttc gcttcgagtc gaggacgcgc atcggatgcc
     1021 ggtcgatctt ggcggcgtcc tcggggtcga gatcgccgac gcgtccgttc agaaacgctc
     1081 gcagggcgtc catgtagcgc gcacggctct cggggtctcc gatcgagttc agctccaacc
     1141 ggagctgcga cagcccgagc gaccggtaga agtcccacag gccgacg
//
LOCUS       DAOOMA010000685         1183 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_160587_length_1183_cov_3.940603, whole genome shotgun
            sequence.
ACCESSION   DAOOMA010000685 DAOOMA010000000
VERSION     DAOOMA010000685.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 1183)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1183)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1183
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_160587_length_1183_cov_3.940603"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            63..209
                     /locus_tag="PLP95_13890"
                     /pseudo
     CDS             63..209
                     /locus_tag="PLP95_13890"
                     /inference="COORDINATES: protein motif:HMM:NF012512.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
     gene            254..454
                     /locus_tag="PLP95_13895"
                     /pseudo
     CDS             254..454
                     /locus_tag="PLP95_13895"
                     /inference="COORDINATES: protein motif:HMM:NF012512.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            500..1174
                     /locus_tag="PLP95_13900"
     CDS             500..1174
                     /locus_tag="PLP95_13900"
                     /inference="COORDINATES: protein motif:HMM:NF012794.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB46940.1"
                     /translation="MKRYAADQPVADQHLGDAPVKQSFVAVTSGANMNFDRLRFVAER
                     SEERESLFAVTIPERRGSFRHLAELLGDRAVTEFNYRISDRDRAVVFVGIATTGSADS
                     AEMAEEFGRSGFDTVDLTHDELATEHLRHMVGGPSALAGNERLFRILFPERPGALGRF
                     LDAMSAEWNISLFHYRNQGADYGRVLIGIQVPPEDDEAFARFLDAMAYPWVEESDHPA
                     YRLFLR"
BASE COUNT          191 a          393 c          385 g          214 t
ORIGIN      
        1 tgcacggcga caactattcc gatgccgccg aacgcgccga ggaactgcgc gtcgccgagg
       61 gcctgaccta tgtgcatccg ttcgacgatc ccgacgtcat cgccggccag ggaacgatcg
      121 gcatggagat tctccgccag gttcacggcg ttcccgatgc gatcttcgtt cccgtcggtg
      181 gaggcggact cgtcgccggc atcggggcgt atgtgaaggc ggtgttgccc gaggtgcgca
      241 tcgtcggcgt ccaatccgag gactccgatg cgatggcccg ttcggtcgat tccggcgagc
      301 tggtcgaact ccccgatgtc ggcctcttag cgacggcacg gcggttcgag ccgtcggaac
      361 cgagacctat cgactggccc gcgaggtcgt cgacgagttc atcaccgtcg acaccgatgc
      421 cgtctgtgcg gccatcaagg acatcttcaa cgacacccgc agcatcgtcg aaccggccgg
      481 agcgttggcc gttgcggggg tgaagcgcta cgccgccgac cagcctgtcg ccgatcagca
      541 cctcggcgac gcgcccgtga agcagtcgtt cgtcgcggtc accagcggcg ccaacatgaa
      601 cttcgatcgc ctgcgtttcg tcgccgaacg atccgaggag cgggaatcgc tgttcgccgt
      661 cacgattccc gagcgtcggg ggagttttcg tcatctggcc gaactgctcg gcgaccgggc
      721 ggtcaccgag ttcaactacc gcatctctga tcgcgaccgg gcggtggtct tcgtgggcat
      781 cgccacgacg ggttcggcgg attcggcgga gatggccgaa gagttcggtc gcagcgggtt
      841 cgacacggtc gatctcaccc atgacgaact cgccaccgaa caccttcgcc acatggtggg
      901 gggtccgagc gcgcttgccg gcaacgagcg cttgtttcgc atcctgttcc ccgaacgccc
      961 tggcgcgttg ggacgctttc tggatgcgat gagcgcagag tggaacatca gcctgttcca
     1021 ctaccgcaac cagggcgccg actacggccg ggtgctcatc ggcatccagg ttccccccga
     1081 ggacgacgag gcgttcgcca ggttcctcga cgcgatggcg tatccgtggg tggaggagtc
     1141 cgaccacccc gcctaccggc ttttcctgcg gtagcgccac cca
//
LOCUS       DAOOMA010000686         1181 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_161017_length_1181_cov_3.482238, whole genome shotgun
            sequence.
ACCESSION   DAOOMA010000686 DAOOMA010000000
VERSION     DAOOMA010000686.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 1181)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1181)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1181
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_161017_length_1181_cov_3.482238"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            175..>1181
                     /locus_tag="PLP95_13905"
     CDS             175..>1181
                     /locus_tag="PLP95_13905"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HPB46941.1"
                     /translation="MNLGVSEEQQALIDSFAQLFAKESNSESVRETEPGGFDPSLWAS
                     LGEVGVTPMGVAEEAGGWGAGMIELALVAEQVGRASAPAPVIESQVAARLLAGLNPQG
                     AAAEVLNAALEGSRILTIAVRPVVGGVATMAPAGAICDAMLVLDGDRLLLATVTDADR
                     TPVANLGDAPLADVRLADGAIELASGADAVERFETAIDEWLTLTANAMVGLGLASQAI
                     ACEYARERQAFGAPIGTFQGISHPLADAATNLDGAQLIARKAAWALAVGHPDARMWAA
                     MAFAFAARSAEHATYTALHTHGGYGFMLEYDVQLHCRRVRGWSRVWGDAEVAYRRAAG
                     SA"
BASE COUNT          172 a          392 c          414 g          203 t
ORIGIN      
        1 catcccagac gggaaagtcg agcggcatgt ggtccccctg tgtcggtgtg tcgacagcgc
       61 acttcgacgt gctgtcgagt cgttgatctc tacggtagtc acgtagtcct agtgaggaaa
      121 gtctcacttt cctcgccggg gaacgccgca gaccccaccg ctatacttcg gcccgtgaat
      181 ctcggcgtca gtgaagaaca acaggccctc atcgactcgt tcgcgcagct cttcgcgaag
      241 gagtcgaact ccgagtcggt ccgcgagacc gaaccgggcg gattcgaccc gtcgctgtgg
      301 gcgtcgctcg gcgaggttgg tgtgacgccc atgggtgttg ccgaggaggc cggcggctgg
      361 ggtgccggca tgatcgaatt ggcgctggtc gccgagcagg tcggccgtgc gagcgctccg
      421 gctccggtca tcgaaagcca ggttgcggct cgcctgctcg ccgggctcaa ccctcagggt
      481 gccgctgccg aggtgctgaa cgctgcgctc gagggcagcc ggatcctcac gatcgccgtg
      541 cgtccggtcg tcggcggggt ggccacgatg gcgcccgcgg gtgcgatctg cgacgccatg
      601 ttggtgctcg acggcgatcg actgttgctc gcgacggtca ccgacgctga ccgcaccccg
      661 gttgcgaacc tcggcgacgc cccgcttgcc gacgttcggc tcgcagacgg cgcgatcgag
      721 ttggcctcgg gtgccgacgc ggtcgaacga ttcgaaacgg cgatcgacga gtggctcacc
      781 ctcaccgcca acgcgatggt tggcctcggc ctggcgtcgc aggcgatcgc gtgtgagtac
      841 gcccgtgagc gccaggcgtt cggtgcgccg atcggaacct tccagggcat ctcccatccg
      901 ctcgccgacg ccgcgacgaa cctcgacggc gcccagctca tcgcccgcaa ggcggcgtgg
      961 gcgctggcgg tcgggcaccc cgatgcccgg atgtgggcgg cgatggcatt cgcgttcgct
     1021 gctcgttcgg ccgaacacgc cacctacacc gcgctgcaca cccatggcgg ttacgggttc
     1081 atgttggaat acgacgtgca actgcactgc cggcgcgtgc gcgggtggag tcgcgtgtgg
     1141 ggagacgccg aggttgcata tcgccgcgcc gccggaagcg c
//
LOCUS       DAOOMA010000687         1180 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_161296_length_1180_cov_2.683556, whole genome shotgun
            sequence.
ACCESSION   DAOOMA010000687 DAOOMA010000000
VERSION     DAOOMA010000687.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 1180)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1180)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1180
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_161296_length_1180_cov_2.683556"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            121..>1180
                     /locus_tag="PLP95_13910"
     CDS             121..>1180
                     /locus_tag="PLP95_13910"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HPB46942.1"
                     /translation="MKPHRSRLAIAALAASALLDTACGEGTTDTGTEAPSDGGVNAST
                     LPDCPVGAFENETGSTKVVLWTSAVGATLATLRSIATDYNASQNKVEVVVETQGIKYE
                     ELRKNFETGVSAKQLPAIVMGEDTWTQYMIDNGVTMPAQACINADDDERAHAYDDMLP
                     GVTAAYTSSEVLWPGAFGASTVVLYFNKNHFTAAGLDPENPPTTYAEVQAAAEQLKAS
                     VGAGNDAYKPLALRLDSWFVENTTSAEGETFVNNDNGRNGKRATESTMNNPATVEMLD
                     WLATMKDEGLLNTVESSSLIDHYLAVATESSSMLLETSTSATLVDQITSSAEGLDISQ
                     IVDEETAKQFEAFNGFKVP"
BASE COUNT          246 a          418 c          341 g          175 t
ORIGIN      
        1 tgattttcca gcgtcaactc gtgcgtggcc tcaccgccgg cgccgtcaag taaccgcccc
       61 ccatcgcgtt cgcacccgtt gcgtcgcgac acaccacgtt cccgactcca ggagaccaac
      121 gtgaaaccac accgttcccg tctggccatc gccgccctcg ccgcctcggc gctgctggac
      181 accgcatgcg gcgaaggcac caccgacacc ggcaccgagg ccccctccga cggaggggtc
      241 aacgcctcga cgcttcccga ttgcccggtc ggagccttcg aaaacgagac cggttccacc
      301 aaggtggtgc tgtggaccag cgccgtcggc gcaaccctcg ccacgctgcg cagcatcgcg
      361 accgactaca acgccagcca gaacaaggtg gaggtcgtcg tcgaaaccca gggcatcaag
      421 tatgaagagc tgcgcaagaa cttcgaaacg ggtgtgtcgg ccaaacaact cccggcgatc
      481 gtgatgggtg aggacacctg gacccagtac atgatcgaca acggcgtcac catgcccgca
      541 caggcgtgca tcaacgccga tgacgacgag cgggcccatg cctatgacga catgttgccc
      601 ggcgtgaccg cggcctacac gagttccgag gtgctgtggc cgggggcgtt cggagcctct
      661 accgtcgtgt tgtacttcaa caagaaccac ttcaccgccg ccggcctcga ccccgagaac
      721 ccgccgacga cctacgccga ggtgcaggcc gccgcggagc agctcaaggc atcggtcggc
      781 gccggcaacg atgcctacaa gccgctcgcg ctccggctcg actcctggtt cgtcgaaaac
      841 accacgtccg ccgagggcga gacgttcgtg aacaatgaca acgggcgaaa cggcaagcgg
      901 gcgaccgaat ccacgatgaa caaccccgcg acggtcgaga tgctcgactg gctggccacg
      961 atgaaggacg agggcctcct caacaccgtc gagtcgtcga gcctgatcga ccactacctc
     1021 gcggttgcaa ccgagagttc ctcgatgctg ttggaaacct cgacctcggc cacgctcgtc
     1081 gaccagatca cttcgagcgc cgagggcctc gatatttccc agatcgtcga cgaggaaacc
     1141 gccaagcagt tcgaggcctt caacggattc aaagtcccct
//
LOCUS       DAOOMA010000688         1166 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_164296_length_1166_cov_3.836184, whole genome shotgun
            sequence.
ACCESSION   DAOOMA010000688 DAOOMA010000000
VERSION     DAOOMA010000688.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 1166)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1166)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1166
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_164296_length_1166_cov_3.836184"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(206..661)
                     /locus_tag="PLP95_13915"
     CDS             complement(206..661)
                     /locus_tag="PLP95_13915"
                     /inference="COORDINATES: protein motif:HMM:NF014016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CbiX/SirB N-terminal domain-containing protein"
                     /protein_id="HPB46943.1"
                     /translation="MNDDAANEPADGPGERVELEPGIDGAAKPAPGAEAEAVILFAHG
                     SRAAEANEWHRHICRELAARTGRRVEPAFLELAMPDLRETALALWREGWRRAVVVPYL
                     LAPGRHAREDLPALVDAINAELGREMVRLNPIFGEDPALLDVLAGQISA"
     gene            679..>1166
                     /locus_tag="PLP95_13920"
     CDS             679..>1166
                     /locus_tag="PLP95_13920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020378390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrmH family RNA methyltransferase"
                     /protein_id="HPB46944.1"
                     /translation="MTLPEPVDGEHHYGGDAPASNDDIGVGPHPQPWPTGEHYDPELL
                     ATGDRRNVIDRYRYWRLDAIVSDLDTRRHPYHVAIENWEHDFNIGTVVRNANAFMAAE
                     VHIVGKKRWNRRGAMVTDRYQHVRHHATVDEFASWCGDAELAIIGIDNLPGSVPIETA
                     ELP"
BASE COUNT          201 a          393 c          375 g          197 t
ORIGIN      
        1 catcgtcgat cccggaaccc aggttattca gttcgctgtc gaagccctcg aacatgcgtt
       61 tgatactaaa cgcgacccct gacaacgaag atccgtcgcc actcgaattt caggagaatt
      121 tcttgagtcg cccaccggtc gcgtcgaaga actcatcgtc gcggcactgg ggagtgtggg
      181 ggccggttgg gaaaccaccg gcccctcacg cgctgatctg acccgccagc acatcgagga
      241 gcgcggggtc ctctccgaag atcgggttca ggcggaccat ctcgcggccc agttcggcgt
      301 tgatggcgtc gacgagcgcg ggcagatcct ctcgtgcgtg gcgacctgga gccagcaggt
      361 agggcacgac cacggctcgg cgccagccct cacgccagag ggcgagcgct gtttcgcgca
      421 ggtccggcat cgccagttcg aggaaggctg gctcgacacg gcgcccggta cgagcggcga
      481 gctcgcgaca gatgtgtcgg tgccactcat tcgcctcggc ggcgcggctt ccgtgtgcga
      541 acaagatgac ggcctcggcc tcggcgcccg gtgcgggctt ggccgcgccg tcgatccccg
      601 gctccaactc cacacgttcg cccgggccgt cggcgggctc gttggcggca tcgtcgttca
      661 ccgcgacaac cttaggacgt gactttgccg gagccggtcg acggcgaaca ccactacggc
      721 ggcgacgctc cggcgtcgaa cgatgacatc ggtgtcggtc cgcaccctca gccctggccg
      781 accggcgagc actacgaccc cgaactcctg gccaccggcg accgccgcaa cgtgatcgat
      841 cggtaccggt actggcgact cgatgcgatc gtctccgacc tcgacacccg tcggcacccc
      901 tatcacgtcg cgatcgagaa ctgggaacac gacttcaaca tcggcacggt cgttcgcaac
      961 gccaacgcct tcatggcggc cgaggtccac atcgtgggta agaagcgttg gaaccgacgg
     1021 ggggcgatgg tgacggatcg ctaccagcac gttcgtcacc acgccacggt cgacgagttc
     1081 gcttcgtggt gtggcgacgc tgagttggcg attatcggga tcgacaatct tcctgggtcg
     1141 gtccccatcg agacggcgga gcttcc
//
LOCUS       DAOOMA010000689         1162 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_165216_length_1162_cov_3.879855, whole genome shotgun
            sequence.
ACCESSION   DAOOMA010000689 DAOOMA010000000
VERSION     DAOOMA010000689.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 1162)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1162)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1162
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_165216_length_1162_cov_3.879855"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..973)
                     /locus_tag="PLP95_13925"
     CDS             complement(<1..973)
                     /locus_tag="PLP95_13925"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HPB46945.1"
                     /translation="MAAPKVLVMILAGGEGKRLLPLTNDRAKPAVPFGGNYRLIDFVL
                     SNFANAQYTRIVVLTQYKSHSLDLHISRTWRFSTLLGSYVSSVPAQMRNGPKWFSGSA
                     DAIFQNLNLITDERPDIVCVFGADHIYRMDPSQMVEYHMSHDRGCTVAAIPVTTQEAT
                     AFGVIEADDEMNVRRFLEKPEEPPTMPGSDSMSLASMGNYVFDTQTLIDAVTPGRLTG
                     LPHDIGGDLMPALAEAGHTKVYDFSTNLVPGQEERERGYWRDVGTIDAYYDANMDLVS
                     SVPVFSLYNTEWQVFTHSPPLPPAKLSRGGSGRLPEVSDTLVGASAIV"
BASE COUNT          215 a          377 c          370 g          200 t
ORIGIN      
        1 acacgatcgc ggaagccccc acgagggtgt ccgacacctc gggaaggcga ccggaaccgc
       61 cccgcgacag cttcgccgga ggcagcggcg gcgagtgggt gaacacctgc cactcggtgt
      121 tgtacaggct gaacaccggc accgacgaca cgaggtccat gttggcgtcg tagtaggcgt
      181 cgatcgtgcc gacgtctcgc cagtacccgc gctcgcgctc ctcctggccc ggaacgaggt
      241 tggtcgaaaa gtcgtacacc ttggtgtgtc cggcctcggc gagcgccggc atgaggtcgc
      301 cgccgatgtc gtggggaagc cccgtgaggc gccccggggt caccgcatcg atcagcgtct
      361 gggtgtcgaa cacgtagttc cccatcgatg ccagcgacat cgaatcggaa ccgggcatcg
      421 tcggcggttc ctcgggcttt tccaagaatc gccgaacgtt catctcgtcg tcggcctcga
      481 tgaccccgaa ggcggtcgcc tcctgggtgg tgaccggaat cgccgccacc gtgcagcccc
      541 gatcgtggct catgtggtac tcgaccatct gggacggatc catccggtag atgtggtcgg
      601 cgccgaacac gcacacgatg tcggggcgtt catcggtgat gaggttcagg ttctgaaaga
      661 tcgcatccgc tgagcccgaa aaccacttcg gaccgttgcg catctgcgcc ggcaccgagg
      721 atacgtagct tcccagcaac gttgagaacc gccacgtccg cgaaatgtgc agatcgaggc
      781 tgtggctctt gtactgggtc aacaccacga tgcgggtgta ttgcgcgttg gcgaagttcg
      841 acaacacgaa atcgatcagc cgatagttgc cgccgaacgg cacggcgggt ttggcccggt
      901 cattcgtgag cggtaacaag cgcttgccct caccgcccgc caggatcatc acaaggacct
      961 tcggtgcagc cacgcactca cactaccggc gacttccggc gttttgaggc gactcagccg
     1021 ctgtttaccg agagttcccc gtagaggtcg cgatatcgca gggccgggcc gtcccaggac
     1081 cagtcgagcg acatggcccg cctgcggaca cgctcgaaac gcgcccggtt cgaaaacagc
     1141 cgagcggcac ggtgcacccc gt
//
LOCUS       DAOOMA010000690         1127 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_173625_length_1127_cov_2.555037, whole genome shotgun
            sequence.
ACCESSION   DAOOMA010000690 DAOOMA010000000
VERSION     DAOOMA010000690.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 1127)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1127)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1127
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_173625_length_1127_cov_2.555037"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..346
                     /locus_tag="PLP95_13930"
     CDS             <1..346
                     /locus_tag="PLP95_13930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015439988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HPB46946.1"
                     /translation="GPDGWEMPVCEVGAEVGDTYRYEWAMPDGSNRFGFTGEVLEVAA
                     PHRVTTTEAMIGSDGPSTRNEMTFTACADGTVLSTVITYPSVELRDAILATGMVDGME
                     ASYARLESSVLG"
     gene            384..1037
                     /locus_tag="PLP95_13935"
     CDS             384..1037
                     /locus_tag="PLP95_13935"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF002003.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003905 - alkylbase DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HPB46947.1"
                     /translation="MITTTGYRETGGGREVDGGRDRWGWLAVDAETAARRLLGCELES
                     VVETPDGEVALRARIVETEAYDETDPASHCFRGPTPRNAAMFLSAGHAYVYLNYGIHR
                     CLNIVCGVAGVGSGVLVRAVEPIAGIEEMRRRRGPRGLVGHELSNGPGKVGAALGIDL
                     DLSGHDLATAPLTLHRHAPGAGPEAGDIAVTPRIGITRAVDAPRRFHLRSSPWVSRP"
BASE COUNT          173 a          370 c          405 g          179 t
ORIGIN      
        1 cggtcccgac gggtgggaga tgccggtgtg cgaggtgggt gccgaggtcg gcgacaccta
       61 ccgctacgag tgggcgatgc cggacggctc gaatcggttc ggattcaccg gcgaggtgct
      121 cgaagtggcg gctcctcacc gtgtgacgac caccgaggcg atgatcggtt ccgacggtcc
      181 ctcgacccga aacgagatga cgttcaccgc atgcgcggac ggcacggtgc tgtccacggt
      241 gatcacctat ccgtcggtag aactccgcga cgcgattctc gccacgggga tggtcgacgg
      301 catggaagcg agctacgccc gtctggagtc gagcgtgctg ggctgatcga tgcccacgac
      361 aggttctgtg ataaccagtg gtcgtgatca cgaccactgg ttatcgggaa acgggtgggg
      421 ggcgcgaggt cgacggggga cgcgaccgct ggggctggtt ggcggtcgac gctgagaccg
      481 cggctcgccg gctgttgggg tgcgaactcg agagcgtcgt cgagaccccc gacggcgagg
      541 tggcgctgcg ggcgcgcatc gtcgagaccg aggcctacga cgaaaccgac cccgcgagcc
      601 actgctttcg tggaccgacg ccgcgcaacg cggcgatgtt tctgtccgcc ggccacgcgt
      661 acgtgtactt gaactacggc atccaccgtt gcctcaacat cgtgtgcggc gttgccggag
      721 tcggttcggg cgtgctcgtt cgggcggtcg aaccgatcgc cggaatcgag gagatgcgcc
      781 ggcgtcgggg accgcggggc ctggtgggcc acgaactcag caacggtccg ggcaaggtcg
      841 gtgcggcgct cggcatcgac ctcgacctca gcggccacga cctagcgacg gccccgctga
      901 cgctgcaccg gcacgcgccc ggggccggtc ccgaagccgg cgacatcgcg gtgacgcccc
      961 gcatcgggat cacccgtgcg gtcgacgcgc cacggcgctt tcatctgcgt tcgagcccct
     1021 gggtgtcgcg cccctgatcg cgccccgccg ctcctcccgg cgactcgccc caccggttct
     1081 ccgataacca gtggtcgtga tcacgaccac tggttatcgg agaacgg
//
LOCUS       DAOOMA010000691         1121 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_175043_length_1121_cov_3.226079, whole genome shotgun
            sequence.
ACCESSION   DAOOMA010000691 DAOOMA010000000
VERSION     DAOOMA010000691.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 1121)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1121)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1121
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_175043_length_1121_cov_3.226079"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..513)
                     /locus_tag="PLP95_13940"
     CDS             complement(<1..513)
                     /locus_tag="PLP95_13940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB46948.1"
                     /translation="MTTPTRPNAGPYADHQNRPPTRTRRLLNLVLILVVVASTCLWAG
                     AVIWGWATDPKPVNWLENREFPEAAEPICAAAVAEIKTFPKAHESKTPTERAAVIRQT
                     TGVLETMIADLREIVPDDADAKWINMWLDDYGIHLADRLDYAERLDGPDGAKEEFFET
                     PKADKQISVSL"
     gene            complement(566..>1121)
                     /locus_tag="PLP95_13945"
     CDS             complement(566..>1121)
                     /locus_tag="PLP95_13945"
                     /inference="COORDINATES: protein motif:HMM:NF015734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PASTA domain-containing protein"
                     /protein_id="HPB46949.1"
                     /translation="MKDALAEVPPTDFDFTIRSSATTTTTIAPTNTEIFEFEGTTETV
                     TMPALNGLNIDQATSRARSAGLTLRRINVDTPPNTGPGMVLNQAPASGSQVSKGATIT
                     VEATAGSPAPTRPVPNIVGLPLNDAAAQLRSAGFIVSHEVVAAPADVVMGDGLAPLPG
                     IVWSVTPAVGTVSIDGKLTLHVQP"
BASE COUNT          154 a          351 c          413 g          203 t
ORIGIN      
        1 gagcgacacc gagatctgct tgtcggcctt cggggtttcg aaaaactcct ccttggcgcc
       61 gtcggggccg tcgaggcgct ccgcgtagtc gagacggtcg gcgaggtgga tgccgtagtc
      121 gtcgagccac atgttgatcc acttcgcgtc ggcgtcgtcg ggaacgatct cccggagatc
      181 cgcgatcatc gtctcgagca cccccgtcgt ctggcgaatc accgcagcgc gctcggtggg
      241 tgtcttggat tcgtgtgctt tcgggaaggt cttgatttcc gcgaccgccg cggcgcagat
      301 cggttcggcc gcctccggga actcgcggtt ctcgagccag ttcaccggct tcgggtccgt
      361 cgcccacccc cagatcaccg ctccggccca caggcaggtc gaggcgacga cgacgaggat
      421 cagtaccagg ttcagcagac gccgggttcg ggtcggtggc cggttctggt ggtcggcgta
      481 gggccccgcg ttcggtcgag tcggggtggt catgtcacgt tcctccgaag gggggagttg
      541 gcggttgcag gccgcggcgg ggccatcagg gctgcacgtg cagggtcagc ttgccatcga
      601 tcgagaccgt gccgacagcc ggggtgaccg accacacgat ccccggcagc ggcgccagcc
      661 catcgcccat gaccacatcg gcgggggcgg cgaccacctc gtggctcacg atgaacccgg
      721 ccgaccgcaa ctgggcggcg gcgtcgttca gtggcagccc gacgatgttg ggaaccggtc
      781 gagtcggtgc cggcgacccg gcggtggcct cgacggtgat cgtcgcaccc ttggagacct
      841 gcgatcccga cgcgggcgcc tggttaagca ccatgccggg cccggtgttc ggtggggtgt
      901 cgacgttgat ccgccgcagc gtgagcccgg ccgacctggc gcgggaggtc gcctggtcga
      961 tgttgagccc gttgagcgct ggcatggtga cggtctcggt ggtgccctcg aactcgaaaa
     1021 tctccgtgtt cgtcggggcg atcgtggtcg tggtggtcgc ggaggagcgg atcgtgaagt
     1081 cgaagtcggt cgggggtacc tcggcgagag cgtccttcat g
//
LOCUS       DAOOMA010000692         1116 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_176294_length_1116_cov_2.866164, whole genome shotgun
            sequence.
ACCESSION   DAOOMA010000692 DAOOMA010000000
VERSION     DAOOMA010000692.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 1116)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1116)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1116
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_176294_length_1116_cov_2.866164"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..588
                     /locus_tag="PLP95_13950"
     CDS             <1..588
                     /locus_tag="PLP95_13950"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HPB46950.1"
                     /translation="LDAISTRRPDLVVLDVMLPDLDGFEVARRVRQHEGAGTKVPIIF
                     LTARDTTSDKVEGLRLGSDDYVTKPFSIEELIERVRAVLRRTGGVGPGSNTLRYADLE
                     LDVDTREVWRGGTLIELTPTEFRLLQFLLANARRVLTREQILEHVWDYTFATNASVLE
                     TYISYLRHKIDAVEPPLIQTVRGVGYTLRLPAGSG"
     gene            680..>1116
                     /locus_tag="PLP95_13955"
     CDS             680..>1116
                     /locus_tag="PLP95_13955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB46951.1"
                     /translation="MNPDDQTTGNHQSTPSPGAAPGIAPGTSKPERSPSPTRTRLVAG
                     TVGAVAALGAVGLAGTIGVNLASAQDDTTSSIAPAESTPDSVPDGAPEKDCGEMGDGM
                     HAGRGMRGRGTAGEISAIDGASLTITDSAGESTTVSTTDDTTVS"
BASE COUNT          211 a          374 c          363 g          168 t
ORIGIN      
        1 ctcgacgcca tcagcaccag acgtcccgat ctcgtcgtgc tcgacgtgat gttgcccgac
       61 ctcgacggct tcgaggtggc caggcgggtt cgccaacacg aaggtgcggg cacgaaggtg
      121 ccgatcatct tcctgacggc gcgagacacc acctcggaca aggtggaagg cctacgcctc
      181 ggctccgatg actacgtgac gaagccgttc agcatcgagg aactcatcga gcgggtgcgt
      241 gcggtgttgc gccgcaccgg tggcgtcggc ccgggatcga acacgctgcg ctatgcggat
      301 ctcgaactcg acgtcgatac ccgcgaggtg tggcgcggcg gaacactcat cgaactcaca
      361 ccgaccgagt tccggctgct gcagtttctg ttggcgaacg cccgtcgggt gctcacccgc
      421 gagcagattc tcgaacacgt ctgggattac accttcgcca cgaacgccag cgtgttggag
      481 acctacatca gctatctgcg ccacaagatc gacgccgtcg agccgccgct cattcagacc
      541 gttcgcggcg tcggctacac cctgaggctg cccgcaggtt cggggtgagt ccggagtgga
      601 tccccagcag attctgagct tcgtgggcga atgtcggtgg tgaaccaggc gtcgaagggc
      661 gccgaaagga gacaccgcca tgaaccccga cgatcagaca acgggcaacc accaatcgac
      721 cccctcaccg ggcgctgcac cgggcattgc accgggtacc tcgaagccag agcgctcacc
      781 ttcgccaaca cggacccggc tcgtcgccgg aaccgtcggc gcagtcgcag cgctcggcgc
      841 ggtggggctt gcgggcacca tcggggtcaa cctcgcttcg gcgcaggacg atacgacctc
      901 gtcgatcgct cccgctgaaa gcacccccga cagcgttccc gacggcgccc ccgagaagga
      961 ctgcggcgaa atgggcgatg ggatgcacgc cggacgcgga atgcgtgggc gtgggacggc
     1021 gggggagatc agcgcgatcg acggggcatc gttgacgatc accgacagcg ccggcgagtc
     1081 gaccaccgtg agcaccaccg atgacaccac cgtgtc
//
LOCUS       DAOOMA010000693         1112 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_177261_length_1112_cov_3.821192, whole genome shotgun
            sequence.
ACCESSION   DAOOMA010000693 DAOOMA010000000
VERSION     DAOOMA010000693.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 1112)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1112)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1112
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_177261_length_1112_cov_3.821192"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            23..>1112
                     /locus_tag="PLP95_13960"
     CDS             23..>1112
                     /locus_tag="PLP95_13960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB46952.1"
                     /translation="MIFGKNLQGSIAEFARHAEQNLGLSRREVQSTLQSFATFGKSAG
                     LDGAPLLKFSEELTQVAADIASLKGVSNEDIMGAFSSALAGEIEPLRRIGINLTDLQK
                     RAEAVKLGLRDAADSSPLTAQQQVLATVSLIKQSPLAMDAEGDFARTIDELPNQMKIA
                     RAEVENLKAEIGEGAVPVFRTLLGGARDLVDVFSALPAPLQQMVGHFAVLGSVGAVAG
                     GSMSVLAGGAIKGSAAVSKLVTKARESEGGIKGMAGSMAGGLNPAMLAGTVAVMGGVA
                     AWEAWSSANAAVEAGARRTTDEIMALTDATEAHAAMVKALREILEGDADSDSAFREIG
                     LGVSEVTKAVSAAPGELDKFRDKVDGLTG"
BASE COUNT          184 a          289 c          412 g          227 t
ORIGIN      
        1 tgacgctatg caggcttccg atgtgatttt cggcaagaac cttcaggggt cgattgccga
       61 gttcgctcgt catgcggaac agaaccttgg cttgtcgcgt cgcgaggtcc agtcgacgtt
      121 gcagtcgttt gccacgtttg gcaagtccgc tggactcgac ggggcaccct tgttgaagtt
      181 ctccgaggag ctcacccagg ttgctgccga catcgcctct ttgaagggcg tgtcgaacga
      241 ggacattatg ggggcgttct cgtcggctct tgccggtgag atcgaaccgt tgcgccgcat
      301 cggcatcaat ttgacggatc tccagaagcg ggccgaggcg gtgaagctcg ggttgcgtga
      361 cgccgccgac agctcgccgt tgacagcgca gcagcaggtg ctggcgacag tgtcgttgat
      421 caaacagtca ccgttggcga tggacgctga gggtgatttc gctcggacga tcgacgagtt
      481 gccgaaccag atgaaaatcg ctcgggctga ggtcgagaac ttgaaagctg agatcggtga
      541 gggtgcggtg ccggtgtttc gcaccctgtt gggcggtgcc cgcgacctgg tcgacgtgtt
      601 ttcggcactg cctgcaccgt tgcagcagat ggtcggacat ttcgctgtgc ttggttctgt
      661 cggggcggtt gctggtggca gcatgtcggt gcttgccggc ggggcgatca aagggtcggc
      721 ggcggtttcg aaactggtaa cgaaggcccg cgaatccgag ggtggcatca aggggatggc
      781 cgggtcgatg gccggcgggt tgaacccggc gatgctcgca ggcacggtcg ccgtgatggg
      841 cggcgtcgca gcgtgggaag cgtggtcgtc tgcgaacgcc gctgtggagg ctggggcgcg
      901 gcgcacgacc gacgagatca tggcgctcac cgacgctacc gaagcgcacg cggcgatggt
      961 gaaagcgttg cgggagatcc tcgaggggga cgccgattcg gattcggcgt ttcgtgagat
     1021 cggcctgggg gttagcgagg ttacgaaagc ggtttcggcg gctccgggcg agctcgacaa
     1081 gttccgtgac aaggtcgacg ggttgactgg tc
//
LOCUS       DAOOMA010000694         1109 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_178081_length_1109_cov_2.855787, whole genome shotgun
            sequence.
ACCESSION   DAOOMA010000694 DAOOMA010000000
VERSION     DAOOMA010000694.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 1109)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1109)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1109
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_178081_length_1109_cov_2.855787"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..813)
                     /gene="der"
                     /locus_tag="PLP95_13965"
     CDS             complement(<1..813)
                     /gene="der"
                     /locus_tag="PLP95_13965"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HPB46953.1"
                     /translation="MMTDTTPTADTTAAPTTATPTLPTVVIVGRPNVGKSTLMNRIIG
                     RREAIVQELPGVTRDRKVVDANWNGRDFLLVDTGGWLAGGTELDQKVSKQSEKALSEA
                     DVVLLVVDATVGVTDDDTEVSRLLRKVDVPVMVVANKVDDTVHEAGMWDLMSLGLGDP
                     FPISALHGRGTGDLLDRVVTYFPELPDPGDLDDLDDDLDDRDALFSVALVGRPNVGKS
                     TLFNRLIGEDRSVVHDMAGTTRDSVDTVVETEHGAIKFIDTAGMRRKSRIDDG"
BASE COUNT          183 a          333 c          385 g          208 t
ORIGIN      
        1 gccatcgtcg atgcggctct tgcggcgcat gccagcggtg tcgatgaact tgatcgcacc
       61 gtgttcggtc tcgacgacgg tgtcgaccga atctcgggtc gtgccggcca tgtcgtggac
      121 caccgagcgg tcctctccga tgagacggtt gaacagggtc gacttgccca cgttcggacg
      181 gccgaccaac gccaccgaaa agagcgcgtc gcgatcgtcg agatcatcgt cgaggtcgtc
      241 gaggtcgcct gggtcgggaa gttcggggaa atacgtcacg acgcggtcga gcaggtctcc
      301 cgtgcctcgg ccgtggagtg cactgatcgg gaacggatcg ccgaggccga gcgacatgag
      361 gtcccacatt ccggcctcgt gcaccgtgtc gtcgaccttg ttggccacga ccatcaccgg
      421 cacatcgacc ttgcgcagca gacgcgacac ctcggtgtcg tcgtcggtca ccccgacggt
      481 ggcatcgacg acgagcagga ccacgtccgc ctcgctcagc gccttttccg actgcttgga
      541 taccttttgg tcgagttcgg tccctccagc gagccaaccc ccggtgtcga cgagcaggaa
      601 atcacgaccg ttccagttgg catcgacgac ctttcggtct cgggtcacgc cggggagttc
      661 ctgcacgatg gcctcgcgcc tgccgatgat gcggttcatc agggttgact tgccgacatt
      721 gggtcggccg acgatcacga cggtgggcag tgtgggggtg gcagtcgtgg gggcggcagt
      781 cgtgtcagct gtgggagtcg tgtcggtcat catcgttcga ttcgattcgg ttcggttcgg
      841 gtgccgggag ccgctcacaa gttggctggc agcagagtcg gcggttgtct ggcagcagcg
      901 tcggcgggtg cggcggaaaa tggtggaagc gtctcggtta ggtggctcgt tggagggccg
      961 cccgcaaggc cactccgtcg gtcgcgatga tctcaacgtc acggggcagg tcatcagggg
     1021 tgagaccatc gaggaagcgc ccacccgaca ggcaaacgtc gggaagaaga tagcggtgac
     1081 cgacgggttc cgccgacagc gcccgttgc
//
LOCUS       DAOOMA010000695         1105 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_179075_length_1105_cov_4.116190, whole genome shotgun
            sequence.
ACCESSION   DAOOMA010000695 DAOOMA010000000
VERSION     DAOOMA010000695.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 1105)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1105)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1105
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_179075_length_1105_cov_4.116190"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..945)
                     /locus_tag="PLP95_13970"
     CDS             complement(<1..945)
                     /locus_tag="PLP95_13970"
                     /inference="COORDINATES: protein motif:HMM:NF015155.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HPB46954.1"
                     /translation="MLARLGSWCFRNRWQTVFAWVGALVALGAIVSALGGARYLESFE
                     LPDSDSSRGIDVLGSEFGELMGGFESSIVVRSNSPVTSSVVQVPLEKLFERVAKLDGV
                     AVSSPFEPGNQDFQISEDGRTAFARLTVNGDFDFTALATLGTQIRDEFPKIKGTQIYL
                     AGEMFAEFEPPKSELIGLAFAIVVLIFAFGSVLAMGLPIAIAVAGVGVGAVVVMLASR
                     LIDVPDLATTIGAMIGLGVGIDYALFIVTRYREQLHAGHEGEEACSIAIDTSGRAVIF
                     AGITVVISLLGMLAIGLSFIGGMGIAAALTVAVTMVASV"
BASE COUNT          230 a          368 c          340 g          167 t
ORIGIN      
        1 caccgaggcg accatcgtca ccgccaccgt gagcgccgca gcgatcccca tcccgccgat
       61 gaacgacagc ccgatcgcca acatgcccag cagtgagatc accacagtga tgccggcgaa
      121 gatcaccgca cggcccgagg tgtcgatcgc gatcgaacag gcctcttcac cctcgtgacc
      181 ggcgtggagt tgctctcgat acctggtgac gatgaacagc gcgtagtcga tgcccacgcc
      241 aagcccgatc atcgcaccga tggtggtggc gagatcgggg acgtcgatga gtcgtgacgc
      301 caacatcacg actaccgctc cgacaccgac gccggcgacc gcaatggcga tcggcagtcc
      361 catcgccagc accgatccga aggcgaagat caacaccacg atggcgaacg caaggccgat
      421 caactcggat ttcggcggtt cgaactcggc gaacatctcg cccgccaggt agatctgtgt
      481 gcccttgatc ttcgggaact cgtcgcggat ctgggtgccg agggtcgcca gggcggtgaa
      541 atcaaaatcg ccattcaccg tcaaccgagc aaacgcggtg cggccgtcct cggagatctg
      601 gaagtcctgg ttgcccggtt cgaacgggga ggacacggcg acgccgtcca gcttcgcgac
      661 gcgctcgaag agcttctcaa gcgggacctg cacgactgaa ctcgtcaccg ggctgttgct
      721 tcgcacgacg atcgaggact cgaagccccc catcagttcg ccgaactccg acccgagcac
      781 atcgatgccg cggctcgagt ccgagtctgg gagttcgaag gactcgagat accgcgcccc
      841 gcccaacgcg gagacgatcg cgccgagcgc gacgagcgct ccgacccagg cgaacacggt
      901 ctgccaccgg ttgcgaaagc accaggaacc aagacgagcc aacatgaagt actccggtgg
      961 tgagggaatc gttcggcgat gagcggccgg tgctggggat tcgccacgcg gccgcgttca
     1021 cctcagttga acggctgttt actctagcaa gcgtgccctt cgatgaagcg aggccgccgg
     1081 cggcctcgct tcatcgaagg gcacg
//
LOCUS       DAOOMA010000696         1104 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_179350_length_1104_cov_3.966635, whole genome shotgun
            sequence.
ACCESSION   DAOOMA010000696 DAOOMA010000000
VERSION     DAOOMA010000696.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 1104)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1104)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1104
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_179350_length_1104_cov_3.966635"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..749
                     /locus_tag="PLP95_13975"
     CDS             <1..749
                     /locus_tag="PLP95_13975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB46955.1"
                     /translation="SDALVQQSDLESSFSESPDLVALIDLVGAEFCGGSVTLDSDPGA
                     RAARAFVDQTNNAMVLSEAVRAKDTTTAGRFVSQMTSLLDDCEGGRYFKVENGERTEV
                     EISNTRRDVPLRLDYVTRTITPVRGGMSQVVTFFQVGNVVVALQYAGPEKAYSSLMDN
                     VERKILTRLAPDQFSETVAIDGEQPMPTDVTTTTRVNIAEPSPTSSVDQTLESLPPAT
                     FEPPTTTSAPRGRSGSAPDSSPDDSSSDGG"
     gene            complement(784..>1104)
                     /locus_tag="PLP95_13980"
     CDS             complement(784..>1104)
                     /locus_tag="PLP95_13980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB46956.1"
                     /translation="QQGFAHHRPEALLLFEADEANHIEPADERSITAKTTALEAHRSQ
                     LETTHFYKLDDAAASTAADAEAAAIETALAGFRERERSKLAEAGAAFGVDYGEAFHLI
                     VDQL"
BASE COUNT          194 a          376 c          345 g          189 t
ORIGIN      
        1 tgtccgacgc gctcgtgcaa caaagcgacc tggaatcctc gttttccgaa tcacccgacc
       61 tggtcgcgct gatcgatctc gtcggcgccg agttctgcgg gggcagcgtc accctcgact
      121 ccgatccggg ggcccgggcc gcccgggcct ttgtcgacca gaccaacaac gccatggtgc
      181 tgagcgaggc ggtccgggcc aaggacacca ccacggccgg gcgtttcgtc tcccagatga
      241 cgagcctgct ggatgactgt gagggcgggc ggtacttcaa ggtcgaaaac ggcgaacgga
      301 ccgaggtgga gatctccaac acccgccgag acgtccccct ccgcctcgat tatgtgaccc
      361 gcaccatcac accggttcgc ggcgggatga gtcaggtcgt cacgttcttc caggtcggaa
      421 atgtggtcgt ggcgctgcaa tacgctgggc cggagaaggc ctattcctcg ttgatggaca
      481 acgtcgaacg caagatcctg accagactcg ccccggatca gttctccgag acggtggcga
      541 tcgacggcga acaacccatg ccgaccgatg tgacgaccac cacccgggtc aacatcgcag
      601 agccgtcgcc gacctcgagt gtcgatcaga ccctcgagag cctgccgccg gccaccttcg
      661 aaccgccgac gacgacctcc gctccacggg gacgatcggg ctccgctcct gattcctccc
      721 cggatgattc ctcctcggac ggcggataac gccgatacgg cgccaaggct ggtcgacgcg
      781 gcgtcagagc tggtcgacga tgaggtgaaa cgcctcgccg taatcgaccc cgaacgcagc
      841 gccggcctca gccagcttcg aacgctcccg ctcacgaaac ccggctaggg ccgtctcgat
      901 cgctgccgcc tcggcatcag cagcggtact cgccgcggca tcgtcgagtt tgtagaagtg
      961 cgtggtttcg agctggctgc gatgggcctc gagcgccgtc gttttcgccg tgatcgagcg
     1021 ctcgtcggcc ggttcgatgt ggttcgcctc gtcggcctcg aacaacagca gcgcttcggg
     1081 tcgatggtgg gcgaaaccct gctg
//
LOCUS       DAOOMA010000697         1103 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_179593_length_1103_cov_4.219466, whole genome shotgun
            sequence.
ACCESSION   DAOOMA010000697 DAOOMA010000000
VERSION     DAOOMA010000697.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 1103)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1103)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1103
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_179593_length_1103_cov_4.219466"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..742)
                     /locus_tag="PLP95_13985"
     CDS             complement(<1..742)
                     /locus_tag="PLP95_13985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine biosynthesis TtcA family
                     protein"
                     /protein_id="HPB46957.1"
                     /translation="MKCTVCREPAVIDLRRHNSNFCAPHFTKFCRDQVAKAVHEFDMI
                     DPQDKVLVAVSGGKDSLAVWDILLDLGYATTGLYIGLGIGEYSDVSKDYARTYAKRRG
                     AELIEVDIVEKYGYDIPDGSKAARRAPCSACGLSKRHIFDDVALDGGFDVLVTGHNLD
                     DEAAVLFGNVMRWQTEYLGRQRPVLEARDGFPKKVKPLIRLGEREMAAYCVVSGIDYV
                     IDECPMAVGNKHIGYKNLLNDLERQSPGT"
     gene            complement(757..>1103)
                     /locus_tag="PLP95_13990"
     CDS             complement(757..>1103)
                     /locus_tag="PLP95_13990"
                     /inference="COORDINATES: protein motif:HMM:NF014636.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HPB46958.1"
                     /translation="RRRTRRTARGEGGPSQTPGSTHPHLPLSRDDARCELADDFTGPA
                     IGQTRGVKVTLRHPTREIEVSGPISVIKLLNRLEFNRESVLLVRNGELVPGDSMLDDD
                     DVIEIRPVISGG"
BASE COUNT          182 a          350 c          353 g          218 t
ORIGIN      
        1 tggtgccggg actctgccgc tcgagatcgt tgagcaggtt tttgtacccg atgtgtttgt
       61 tgcccaccgc catcgggcac tcgtcgatga cgtagtcgat gccgctgacg acgcagtagg
      121 ccgccatctc gcgttccccg aggcggatga gcggcttgac cttcttcgga aatccgtcgc
      181 gggcctcgag cacgggccgt tgccgaccga ggtactccgt ttgccagcgc atcacgttgc
      241 cgaacagcac cgccgcctca tcgtcgaggt tgtggccggt gacgagcacg tcaaagcctc
      301 cgtcgagtgc gacgtcgtcg aagatgtgtc gcttcgacag cccgcacgcc gaacacggcg
      361 cccgacgcgc ggccttcgac ccgtcgggga tgtcgtatcc gtacttctcg acgatgtcga
      421 cctcgatgag ttccgcgcca cgacgcttcg catacgttcg ggcgtagtcc ttggagacgt
      481 cgctgtactc gccgattccc aggccgatgt agagccccgt cgtcgcgtag ccgaggtcca
      541 acaagatgtc ccatacggcg agggagtcct tgccgcccga cactgcgacc agcaccttgt
      601 cctgcgggtc gatcatgtcg aattcgtgaa ccgccttggc gacctgatcg cggcaaaact
      661 tcgtgaaatg cggcgcgcag aagttggagt tgtggcggcg tagatcgatg acggctggtt
      721 cgcggcacac cgtgcacttc atggcgctgt cgcctcctaa ccgccgctga tgaccgggcg
      781 gatctcgatg acgtcgtcgt cgtcgagcat cgaatcgccg gggacgagtt cgccattgcg
      841 tacgagcaac accgactcac ggttgaactc gagtcggttc aacaacttga tgacactgat
      901 gggccccgag acctcgatct cgcgggttgg gtgacgcaac gtgaccttca cgcccctagt
      961 ttggccgatc gccgggccgg tgaaatcatc ggcgagttca cagcgcgcat cgtcgcggct
     1021 cagcggtaga tgcgggtgcg tggaacccgg cgtttgcgag ggcccgcctt cgccgcgcgc
     1081 agtgcggcga gttcggcgtc gag
//
LOCUS       DAOOMA010000698         1089 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_183256_length_1089_cov_3.961315, whole genome shotgun
            sequence.
ACCESSION   DAOOMA010000698 DAOOMA010000000
VERSION     DAOOMA010000698.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 1089)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1089)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1089
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_183256_length_1089_cov_3.961315"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            91..>1089
                     /locus_tag="PLP95_13995"
     CDS             91..>1089
                     /locus_tag="PLP95_13995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0140663 - ATP-dependent FeS chaperone
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mrp/NBP35 family ATP-binding protein"
                     /protein_id="HPB46959.1"
                     /translation="MSSSEAALTESAVLDVLSTVVDPELRRSIVELGQVDEVAVAGDA
                     VRLLILTTTPNCPLQAGFDAEIREKLAAIGAVDIQLEFHPMDDEEQRVLRLKLIGDPA
                     STAGSQQAHGHAEGRSIPFSDPAVKTRVLLIASGKGGVGKSSVTTNLAVAMARRGKRV
                     AIVDADVWGFSIPKMIGVTRPPRLIDQMIVPPVANGVSVMSMGFFAEENQAVIWRGPM
                     LHKALEQFLTDVFWDEPDYLLIDLPPGTGDISISLAQFLPRAEMYVVTTPNSASQRVA
                     QRAAAMGHKVNLPVRGVIENMSWFTGDDGKRYELFGSGGGQELADGIGVALLGRIPL"
BASE COUNT          182 a          366 c          345 g          196 t
ORIGIN      
        1 acatcggccc ggggtatcgg ccgctgcaga ccccaccgac gggttcggac cagatttggg
       61 tgccgcacac ccatgggaaa gaatggccac atgtcgtctt ccgaagcagc gctcaccgaa
      121 tcagcggtcc tcgatgttct ctcgaccgtc gtcgaccccg agttgcgtcg ttccatcgtc
      181 gaactcggcc aagtcgacga ggttgcggtc gctggcgacg cggttcgtct cctgatcctg
      241 accacgacac cgaactgccc gcttcaggcc ggcttcgacg ccgagatccg cgagaagctc
      301 gccgcgatcg gtgccgtcga tatccagctc gaattccatc cgatggatga tgaggaacag
      361 cgggtgttgc gcctcaagct catcggcgat ccggcctcga ccgccggttc gcaacaggca
      421 cacggccacg ccgaggggcg ctcgatcccg ttctccgacc cggcggtgaa aacccgggtc
      481 ctgctcatcg cgtcgggcaa gggcggcgtc ggcaagtcgt cggtcaccac gaaccttgcg
      541 gtggccatgg cccgccgcgg caagcgggta gcgatcgtcg acgcggacgt gtggggattc
      601 tcgattccga agatgatcgg ggtgacacgc ccgcctcggc tcatcgatca gatgatcgtg
      661 ccaccggtgg ccaacggcgt gtcggtcatg tcgatggggt tcttcgccga ggagaatcag
      721 gccgtcatct ggcgtggccc catgttgcac aaggccctcg aacagttcct taccgacgtg
      781 ttctgggacg agcccgacta cctcctcatc gaccttccgc ccggcaccgg cgacatctcg
      841 atctcccttg cacagttcct gccccgagcc gagatgtacg tcgtcaccac cccgaactcg
      901 gcatcgcagc gcgtcgcgca gcgggctgcc gccatgggtc acaaggtcaa cctgccggtg
      961 cgcggcgtca tcgagaacat gagctggttc accggcgacg acggcaagcg ctacgagctg
     1021 tttggttcgg gtggtggtca ggagctcgcc gacggcatcg gcgtagcgct gctgggccgg
     1081 atccctctg
//
LOCUS       DAOOMA010000699         1088 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_183566_length_1088_cov_3.283640, whole genome shotgun
            sequence.
ACCESSION   DAOOMA010000699 DAOOMA010000000
VERSION     DAOOMA010000699.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 1088)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1088)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1088
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_183566_length_1088_cov_3.283640"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..422
                     /locus_tag="PLP95_14000"
     CDS             <1..422
                     /locus_tag="PLP95_14000"
                     /inference="COORDINATES: protein motif:HMM:NF013117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HPB46960.1"
                     /translation="CHTVPLRSRNWVLLNGTVWHVPNGTILTVGNKSQGWARAVVEVT
                     IALSADIAAARRTMIEVAETMAREERWRDAMALDAGPDDQGVSALTPVGATLRIVVTT
                     EPKSQWAVERELRQRIKEAFDAAGISLEVPHIAPPVL"
     gene            490..666
                     /locus_tag="PLP95_14005"
     CDS             490..666
                     /locus_tag="PLP95_14005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB46961.1"
                     /translation="MQKQLGGAGLLALLIVGYLIWKDPTGTANAISGFFGAVGDFAST
                     LWDKLGEFITSLVG"
     gene            666..>1088
                     /locus_tag="PLP95_14010"
     CDS             666..>1088
                     /locus_tag="PLP95_14010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB46962.1"
                     /translation="MDDRWHRPWLMRLLPDRRIERFLSEGEHVLRDTRPSFKVWFLGV
                     FDVIAAGVLMLAVVVAAADVAVTKMALVLWAVLAVHLVVEFVRFAFTRYVVTDHRAIS
                     WSGVYHDRFEWISWRKLTDVSVHSSLGERLLKTATIRVH"
BASE COUNT          168 a          339 c          385 g          196 t
ORIGIN      
        1 catgccacac cgtgccgttg agatcgcgca actgggtgct tctcaacggc acggtgtggc
       61 atgtccccaa cggcacgatc ctcaccgtcg gaaacaagag ccaggggtgg gcccgagcgg
      121 tggtcgaggt caccatcgca ctgtcggccg atatcgccgc ggcgcgacgg accatgatcg
      181 aggtggccga gacgatggcg cgggaggaac gctggcgcga tgccatggcg ctcgacgccg
      241 gccccgacga tcagggggtg tccgcactga ccccggtggg cgcgacgttg cggatcgtgg
      301 tgaccaccga gccgaagtcg caatgggccg tcgagcgcga gctgcgccaa cgcatcaagg
      361 aggccttcga cgccgccggc atcagcctgg aggtccccca catcgcgccg ccggttcttt
      421 aggctcgcga ggtggcccag gtccgcaatg gacgcgtcgt gggcagccga tgatgcgagg
      481 aggtgaccga tgcagaagca actgggcgga gcggggctgt tggcgctgct catcgtcggt
      541 tacctcatct ggaaggaccc gacgggcacc gcgaatgcca tctcggggtt cttcggtgcg
      601 gtcggcgatt tcgcgtcgac gctgtgggac aagctcggag aattcatcac cagcctggtg
      661 ggctgatgga tgaccgctgg catcgcccgt ggttgatgcg gctcctgccg gatcgtcgca
      721 tcgagcgatt tctcagcgag ggcgagcacg tgctgcgaga cacgcgaccg tcgttcaagg
      781 tgtggttcct gggcgtgttc gatgtgatcg ccgccggggt gctgatgctc gcggtcgtcg
      841 tggcggcggc cgacgtcgcg gtcaccaaga tggcgctggt gctgtgggcc gtgttggcgg
      901 ttcacctcgt cgtcgaattc gtccggttcg ccttcacccg ctacgtcgtc accgaccatc
      961 gagcgatcag ttggtccggg gtgtaccacg accgcttcga gtggatctcc tggcgcaagc
     1021 tcaccgatgt ttcggtgcac tcgtcgctcg gcgagcgcct gctcaagacg gccacgatcc
     1081 gcgtccac
//
LOCUS       DAOOMA010000700         1081 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_185443_length_1081_cov_3.653021, whole genome shotgun
            sequence.
ACCESSION   DAOOMA010000700 DAOOMA010000000
VERSION     DAOOMA010000700.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 1081)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1081)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1081
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_185443_length_1081_cov_3.653021"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            172..>1081
                     /locus_tag="PLP95_14015"
     CDS             172..>1081
                     /locus_tag="PLP95_14015"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF016041.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HPB46963.1"
                     /translation="MNPAVLAIPLYFALMWWEVRALKRQRREGRDVLGYDRRDASASI
                     RLGVVSLASVGALNVVSLLLSQWLWQFRLIDLGTGVAAWVVAMIGWDFAYYWLHRFEH
                     QSRLLWAAHVNHHSSEYYNLSTALRQALLPVAAIVFFPPLALIGVRPWIIAVSGGFNL
                     VYQYWIHTEAIDKLPRWFEFVFNTPSHHRVHHGSQRQYLDRNHGGILIIWDRIFGTFQ
                     AEGERVRYGLTKNIHSYSLWTIFSHELVAIWHDVGSTRSWRERWNYTFAGPGWKPPRL
                     KGAQSSANASAMPAAPTRAAITRRRRD"
BASE COUNT          180 a          333 c          351 g          217 t
ORIGIN      
        1 cgcctcgccc cgctcgtcga cttggcgatc ctgtcgatcc aggggctggt gctgaaccag
       61 atggccgctc cggaccccgg tcaggtggag cgacttcgca acatgttgga cgagctcgtc
      121 gagttcgtcg tttcgtaacg gaccagggga ggaaccgtgt tggaggaact gatgaacccg
      181 gcggtgctgg cgattccgct gtacttcgca ctgatgtggt gggaggtgcg tgcgttgaaa
      241 cgccagcgac gtgagggtcg cgacgtgttg ggatacgacc gacgagacgc ctccgcgagc
      301 atccgcctcg gggtggtgtc actcgcgagc gtcggtgcgt taaacgtggt gtcgctgctg
      361 ttgtcgcagt ggctgtggca gttccgcctc atcgacctcg gcaccggtgt tgccgcgtgg
      421 gttgtcgcca tgatcgggtg ggacttcgcc tactactggc tgcatcgctt tgagcatcag
      481 tcgcgactgt tatgggcggc gcacgtcaat catcactcca gcgagtacta caacctgtcg
      541 accgcgctgc gtcaggcgct gctgccggtt gcggcaatcg tgttcttccc gccgctcgcc
      601 ctcatcgggg ttcgcccgtg gatcatcgcg gtgtcgggcg ggttcaacct cgtctaccag
      661 tactggatcc ataccgaggc gatcgacaag ctgccgcgtt ggttcgagtt cgtgttcaac
      721 acgccgtcgc atcatcgggt ccaccacggc agccaacgcc agtacctcga ccgtaaccac
      781 ggcggcatcc tcatcatctg ggaccgcata ttcggtacct tccaagccga gggcgaacgg
      841 gtccgttacg gactgaccaa gaacatccac tcgtactcgt tgtggacgat cttcagccac
      901 gaactcgtcg cgatctggca cgacgtcggt tcgacgcggt cgtggcgtga gcggtggaac
      961 tacacctttg ccggtccggg gtggaagcca ccgagactca agggcgctca gtcgagcgcg
     1021 aacgcctcgg caatgccggc cgctccgacg agggcagcga tcaccaggcg acggcgagat
     1081 a
//
LOCUS       DAOOMA010000701         1075 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_187124_length_1075_cov_3.705882, whole genome shotgun
            sequence.
ACCESSION   DAOOMA010000701 DAOOMA010000000
VERSION     DAOOMA010000701.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 1075)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1075)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1075
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_187124_length_1075_cov_3.705882"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..642)
                     /locus_tag="PLP95_14020"
     CDS             complement(<1..642)
                     /locus_tag="PLP95_14020"
                     /inference="COORDINATES: protein motif:HMM:TIGR03570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HPB46964.1"
                     /translation="MTDTARDLVIVGAGGHGRELASVVASNNQVEPHWNLLGFVDDGS
                     PDLERLARIDVTFLGTLSWLIEHPGIYALGIGSSPTRCSLSQQLDAAGCTVATIVSPY
                     ARLGLDVRLGDGVVIYDNSVLTTNVTIGRHSHINVGCSVQHDSTIGEFVQMSPGVFVN
                     GDVTVGNDVFLGTGAIVTRGVTIGDSARIGAGAVVLDDVAPDTLVVGAPARPVH"
BASE COUNT          180 a          301 c          396 g          198 t
ORIGIN      
        1 gtggacgggg cgggcggggg cgccgaccac gagggtgtcg ggggcgacgt cgtcgagcac
       61 gaccgctccg gctccgatgc gggcgctatc accgatggtg accccacggg tgacgatcgc
      121 gccggtgccc aggaacacgt cgttgccgac ggtgacgtcg ccgttgacga acacgccggg
      181 gctcatctgt acgaactcgc cgatggtcga gtcgtgctgg actgagcatc cgacgttgat
      241 gtggctgtgg cgtccgatgg tgacgttggt ggtgagcacc gagttgtcgt agatgaccac
      301 gccgtcgccg aggcgtacgt cgaggccgag acgggcgtag ggcgacacga tggtggcgac
      361 ggtgcacccg gcggcatcga gttgttgaga aagcgagcag cgggttggtg aggatccgat
      421 tccgagtgcg tagatgccag ggtgttcgat gagccacgac agcgtgccga ggaacgtcac
      481 gtcgattcgg gcgagtcgtt cgaggtctgg tgagccgtcg tcgacgaacc cgagcaggtt
      541 ccagtgcggt tcgacctgat tgttggacgc gacgaccgac gcgagttcac gtccgtgccc
      601 accggcaccg acgatgacga gatcacgcgc ggtgtcggtc atggttgggt tcggtaggta
      661 gcgggcgtcg tgtccatgaa tcgagcgtat cgagcgatga ggatgggacg tcccggccgc
      721 gtggatgtgg gcaccctgtc ggaggtgtcg gtggcctcgg ttgccggcac agcacggccg
      781 cagtgagtgc gagcatcgtc acgacgatgg tgccgaccat ccacctcgtc tgagcggcga
      841 agttctcgga catcagcttg gtgacgcggt cctcgaagct gtcgaaacgg gcgtcgatcc
      901 tggctttcag gttctcgacg tccgctctca gtaacgcgat gtcggctttg tcgccgcggt
      961 atccgcggct cccgacagcg tccaccaatt cgcgagccag ctgggcaggt cggcaattcc
     1021 gataaccagt ggtcgtgatc acgaccactg gttatcggag aacaggtgag gtggc
//
LOCUS       DAOOMA010000702         1073 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_187691_length_1073_cov_3.708251, whole genome shotgun
            sequence.
ACCESSION   DAOOMA010000702 DAOOMA010000000
VERSION     DAOOMA010000702.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 1073)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1073)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1073
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_187691_length_1073_cov_3.708251"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..576
                     /locus_tag="PLP95_14025"
     CDS             <1..576
                     /locus_tag="PLP95_14025"
                     /inference="COORDINATES: protein motif:HMM:NF012903.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin"
                     /protein_id="HPB46965.1"
                     /translation="KAIADELRATAETMIGTANATKAGRQIFSGTADVATAYALDGSY
                     LGNATPVTIPVFDSIAIRVDRMGPEVFGVYDAGDPSAGNIFQQLRWMADEIEAGNVAV
                     AASGLGTIDHALERLATAQVELGTRHAQLESIQANIAGSKVSTTAAISKNEDVDAAEA
                     IMNWRTRQSAYESALKVSAQTMQQSLLDFIR"
     gene            677..>1073
                     /locus_tag="PLP95_14030"
     CDS             677..>1073
                     /locus_tag="PLP95_14030"
                     /inference="COORDINATES: protein motif:HMM:NF013478.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliR"
                     /protein_id="HPB46966.1"
                     /translation="MSLDPQFFLAFMYAMIRCSAWLFLAPPLRGNAPVMVRSGLAMSL
                     GLVMAPRLLNGPEPLETDLGSILAGTLHNAVIGLVLAFVVFMLFSAVSAAGSMIDTFS
                     TLSSAQLFDPMQAAAAGPVRGGGEFRPRMT"
BASE COUNT          175 a          353 c          349 g          196 t
ORIGIN      
        1 aaggccatcg ccgacgaact ccgtgcgacg gcggagacga tgatcgggac ggcgaatgcg
       61 accaaggccg gccgccagat cttctcggga actgcggatg tcgcgaccgc gtacgccctc
      121 gatggctcgt atctcggcaa cgcgacgccg gtcacgattc cggtgttcga ttcgatcgcc
      181 atccgggtcg accgcatggg tccggaggtg tttggggtct acgacgccgg cgacccctcg
      241 gcgggcaaca tcttccaaca gcttcggtgg atggccgatg agatcgaggc gggcaacgtg
      301 gcggtggcgg cctccgggct cggcacgatc gaccacgcgc tcgaacgcct cgcgaccgct
      361 caggtggagc tcggaacacg ccacgcgcaa ctcgaatcca ttcaggcgaa catcgccgga
      421 tcgaaggttt cgaccacggc tgcgatctcc aagaacgagg acgtcgatgc cgccgaggcg
      481 atcatgaact ggcgcacacg ccaaagcgct tatgagtcgg cgttgaaggt ctccgctcag
      541 acgatgcaac agagcctgct cgacttcatt cgctagctcc gcgttccccg actgctcatc
      601 tatgcggcgg caccgccgat agtgaggtga tgtccgagac ggtcgactgc gcatcgtcgg
      661 cgaggccgcc gtgcggttga gcctcgaccc tcagttcttc ctcgcgttca tgtacgccat
      721 gatccgctgc agcgcgtggt tgtttctcgc accgccgttg cgcggcaacg ccccggtcat
      781 ggtccggtcg gggctcgcga tgtcgctcgg gctggtgatg gcgccacggc tcctcaacgg
      841 tccagagcca ctcgagaccg acctcggttc gatcctggcc gggacgctgc acaacgccgt
      901 gatcggcctg gtgctggcgt ttgttgtgtt catgttgttc agcgccgtct ccgcggcggg
      961 atcgatgatc gataccttct cgaccctgag ctccgcccag ctgttcgacc cgatgcaggc
     1021 cgcggccgcc ggcccggtca ggggcggcgg agaatttcga cccaggatga ctc
//
LOCUS       DAOOMA010000703         1047 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_195408_length_1047_cov_2.882056, whole genome shotgun
            sequence.
ACCESSION   DAOOMA010000703 DAOOMA010000000
VERSION     DAOOMA010000703.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 1047)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1047)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1047
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_195408_length_1047_cov_2.882056"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(112..1014)
                     /locus_tag="PLP95_14035"
     CDS             complement(112..1014)
                     /locus_tag="PLP95_14035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB46967.1"
                     /translation="MSRIALVTTDPGEIGFDDPDLIPLLEAIAAAGHEVTTASWRDDS
                     APWERFDLAVIRSPWDYMHHERRFLEWLEAVAQRTSLWNPPELIAWNLNKRYLLELDV
                     HGVPVVPTLLATSPSEVGEAIEAIRDSGFDDVVVKPTVSAGSIDTGLFAANDPAAMEL
                     ARHILKIGKTVMVQPAIPSVAEVGERALVFFDGELSHAFSKGPLLTHGGGLRSGTYTE
                     VLEFVTATVDEIDVASHAMEVIARIAPGLDGAAPLYGRLDLVATDDGPLLLEAELFEP
                     SYNVWLAEGSAQRFVAALERRLDH"
BASE COUNT          184 a          383 c          321 g          159 t
ORIGIN      
        1 atcacagcgc acgacgccgg cgcctgcgcc tcctgatcgc taccggtttc ggcagccggt
       61 ttcggcagcc ggtttcggca gccggtttcg gcagccggct cagtggtcga gtcagtggtc
      121 gaggcgacgc tcgagggctg cgacgaaccg ttgcgccgac ccctccgcca gccacacgtt
      181 gtaggagggt tcgaacagct cggcctccaa caacagcgga ccgtcgtcgg tggcgaccag
      241 gtcgaggcgc ccatacaacg gcgccgcgcc atcgaggccc ggggcgatcc gcgcaatgac
      301 ctccatcgca tgggacgcaa catcgatctc atccaccgtc gccgtcacga actcgagtac
      361 ctcggtgtag gtgcccgagc gcagtccccc gccgtgcgtc aacagcgggc ccttgctgaa
      421 cgcatgtgac aactcgccgt cgaagaacac cagcgcccgc tcgccgacct cggccaccga
      481 cggaatcgcc ggctgcacca tcaccgtctt gccgatcttc aggatgtgac gagcgagctc
      541 catcgccgcc ggatcgttcg ccgcaaacaa ccccgtgtcg atcgaaccgg ccgacaccgt
      601 cggcttcacc acgacatcgt cgaagcccga atcacggatc gcctcgatcg cctcgccgac
      661 ctccgacggc gacgtcgcca acagcgtcgg cacgaccggc accccgtgca cgtcgagttc
      721 cagcagatac cgcttgttga ggttccaggc gatcagctcg ggcggattcc acaacgacgt
      781 gcgctgcgcc accgcttcga gccattcgag aaaccgccgc tcgtgatgca tgtagtccca
      841 cgggctgcgg atcaccgcca agtcgaagcg ctcccacggt gccgagtcgt cgcgccaact
      901 cgccgtggtg acctcgtggc cggccgcagc gatggcttcg agcagcggga tgagatcggg
      961 atcgtcgaag ccgatctccc ccgggtcggt ggtaacgagc gcgatgcgag acacgagcgc
     1021 aggctagttc cgccgcgcgc ctgcccc
//
LOCUS       DAOOMA010000704         1020 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_204018_length_1020_cov_2.759585, whole genome shotgun
            sequence.
ACCESSION   DAOOMA010000704 DAOOMA010000000
VERSION     DAOOMA010000704.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 1020)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1020)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1020
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_204018_length_1020_cov_2.759585"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(203..>1020)
                     /locus_tag="PLP95_14040"
     CDS             complement(203..>1020)
                     /locus_tag="PLP95_14040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020376909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="anthranilate synthase component I family
                     protein"
                     /protein_id="HPB46968.1"
                     /translation="VAKEYIRSGDIFQVVLAQRFDFEVDADPFDVYRVLRQINPSPYM
                     YFVREPELSLVGCSPEPMVQLLGGQVISRPIAGTRFRGSTDEHDRRLAAELLEHPKER
                     AEHIMLVDLARNDVGRVVEFGTCKVDELMTLERFSHVMHLTSQVSGALAEGRTPIDVL
                     KATLPAGTVSGAPKVRAMEIIDELEPTKRGPYAGVVGYLDFSGNIDTAIAIRTMIIDT
                     ADGEMPFRASVQAGAGVVADSVAADEELETRNKAKALLAAVRPAERMTAQRTR"
BASE COUNT          170 a          360 c          317 g          173 t
ORIGIN      
        1 caactctccc tcaccccgac caaaccaccc caaccaccga tacgccaatg aaccgacgac
       61 cccaccgact caggaaccgg ctcgggcctc cgaggaactc cgcgacgtca ccgcgctgtc
      121 ccgcgggcac gcgaccgcgc ctttgttgcg gcgttcggcg gggacgctga tcgcctcgtg
      181 cgctgactac ctcgtgcgct gactacctcg tgcgctgggc ggtcatccgt tcggccgggc
      241 gcaccgcggc gagcaaggct ttcgccttgt ttcgggtttc gagttcctcg tccgccgcaa
      301 cggaatcggc caccaccccg gcgccggcct gcacgctggc ccgaaacggc atctcgccgt
      361 cggcggtgtc gatgatcatc gtgcggatcg cgatggcggt gtcgatgttg cccgaaaaat
      421 cgagatatcc gacgaccccc gcatacggac cacgcttggt gggctcgagt tcgtcgatga
      481 tctccatcgc ccggaccttg ggcgcccccg acacggtgcc ggcgggcagt gtggccttca
      541 acacgtcgat gggcgttctg ccctcggcga gggcgcccga cacctgactc gtcaggtgca
      601 tgacgtggct gaagcgctcg agggtcatga gctcgtcgac cttgcacgtc ccgaactcca
      661 cgacgcggcc gacgtcgttg cgggccaggt cgaccaacat gatgtgctcg gcgcgttcct
      721 tggggtgctc caacagctcg gccgcaagtc gacggtcgtg ctcatcggtc gagccgcgga
      781 accgggtccc ggcgatcggc cggctgatca cctgcccgcc gagcagttgc accatcggtt
      841 cgggtgaaca acccaccagc gacagctccg gttcgcgcac gaaatacatg tacggcgacg
      901 ggttgatctg acgcagcacc cggtacacgt cgaaggggtc ggcgtcgacc tcaaaatcga
      961 agcgctgggc cagaaccacc tggaagatgt caccgctgcg gatgtactcc ttggcgacct
//
LOCUS       DAOOMA010000705         1019 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_204258_length_1019_cov_3.908714, whole genome shotgun
            sequence.
ACCESSION   DAOOMA010000705 DAOOMA010000000
VERSION     DAOOMA010000705.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 1019)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1019)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1019
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_204258_length_1019_cov_3.908714"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            230..>1019
                     /locus_tag="PLP95_14045"
     CDS             230..>1019
                     /locus_tag="PLP95_14045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HPB46969.1"
                     /translation="MTEPSTSQDGAVALAASAPLLETVDVVKEFPVMGGFIRHRVGTV
                     HAVSGVSLTLDASETLGIVGESGCGKSTLGRVVLNLLSKTSGSIRIDGVDTATIADKE
                     LRRQAQMVFQDPYASLNPRMTIGEVLAEPFLVHQRKRAAESREEVAELLRTVGLSPEH
                     ASRYPHEFSGGQRQRIGIARALAVAPKFIVLDEPVSALDVSIQAGIVNLLERLQDERG
                     ISYLFIAHDLSVVRHISHRVGVMYLGKLVEIGTQEEIYELPRHPY"
BASE COUNT          167 a          301 c          361 g          190 t
ORIGIN      
        1 gcgcgaggtc ggcctcgccg aatcgcgcat cttcgatcgc tcggcgctcg accggatgtg
       61 cggacgtgat gccggagttg accgagctcg cgccggcggg cagtagcggt ggtggtcacg
      121 gtggtgttga cggcgctgga gaagcggacg ctgcggctgc catgggttcg caccaggtgg
      181 cgtgtcatcg ccgtgatgag gcggcggtca tgatcgaacg aggtgagcga tgaccgaacc
      241 ctccacgtca caggacggcg cggtggcgct cgcggcgtcg gccccgttgc tcgagacggt
      301 cgacgtcgtc aaggagttcc cggtgatggg cgggttcata cgccatcgcg tgggcacggt
      361 gcacgcggtc agcggcgtgt cgttgaccct cgatgcgtcg gagaccctcg gcatcgtggg
      421 cgagtcgggt tgcgggaagt cgaccttagg tcgagtggtg ctcaatctgt tgtcgaagac
      481 gagcggatcg atccggatcg atggcgtcga caccgcaaca atcgccgaca aggagctgcg
      541 ccgccaggca cagatggtgt ttcaggatcc atatgcgtcg ctgaatccgc gcatgacgat
      601 cggcgaggtt ctggccgagc cgttcctggt gcaccaacga aagcgcgccg cggagtcccg
      661 cgaggaggtc gccgaactgc tgcgaaccgt ggggctgtcg ccggaacatg cgagccgtta
      721 tccccatgag ttttcgggtg gtcagcgtca gcgcatcgga atcgcgcggg cgttggcggt
      781 cgcccccaag ttcatcgtgc tcgacgagcc agtttcggca cttgatgtgt cgattcaggc
      841 cggaatcgtg aacttgctcg aacggctcca ggacgagcgt ggcatctcct atctgttcat
      901 cgcccacgat ctgtcggtgg tgcgtcacat ctcgcatcgg gtcggggtca tgtacctcgg
      961 caagctcgtc gagatcggca cccaggagga gatctacgag ctgccgcgcc acccctaca
//
LOCUS       DAOOMA010000706         1015 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_205572_length_1015_cov_4.412500, whole genome shotgun
            sequence.
ACCESSION   DAOOMA010000706 DAOOMA010000000
VERSION     DAOOMA010000706.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 1015)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1015)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1015
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_205572_length_1015_cov_4.412500"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            176..>1015
                     /locus_tag="PLP95_14050"
     CDS             176..>1015
                     /locus_tag="PLP95_14050"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPB46970.1"
                     /translation="MTSPIIVGGGVVKRYGDNLVLNRTDFSVEPGVTGLLGANGAGKT
                     TLIGMVLGLHRPDAGELRVFGLDPAQHGFEVRGRIGYAPEHHNLPDDLPANDFVQHIA
                     EVHGLPTEAALGRASDALWFVGLGEERFRPLGTLSTGQRQRVKLAMAIAHDPDLVVLD
                     EPTDGLDPTQREAMLSLIRRLAVEFNMSVLLSSHLLDEVERTCDRVVILNGGRVTASG
                     ELSALKGEGSGVSVELLEGAESFARRMSARGFEVSCDGPLVVTRSGSSGASQHDLQRI
                     IRDD"
BASE COUNT          165 a          337 c          341 g          172 t
ORIGIN      
        1 gcgaatgtcg actggctcga aggggtgctc ggacattcgg tgccggggta cctgtcgaaa
       61 gcgggcaact tcccggccct ctcgtactga cgccaccacc aacgccgctc ctctgaacag
      121 agtcttcaca cgcagcgcaa cgatccgcgc tgagccgtgt gcaaaactct gcgccgtgac
      181 ttccccgatc atcgtcggcg gtggcgttgt gaaacgctac ggcgacaacc ttgtgctgaa
      241 tcgaaccgat ttctcggtcg agccgggggt gaccgggctg ctcggtgcca acggtgcagg
      301 caagaccacc ctgatcggca tggtgctggg gttgcatcgc ccagatgccg gcgaactgcg
      361 ggtgttcggg ctcgacccag cccagcacgg cttcgaggtg cgcggccgga tcggctacgc
      421 ccccgagcat cacaacctgc ccgacgacct tcctgcgaac gactttgtgc aacacatcgc
      481 cgaggtccac gggctgccca cggaagcggc gctcggccgg gcatcggacg cactgtggtt
      541 cgtcggcctc ggcgaggagc ggtttcgccc gctcggcacg ctgtcgaccg gccagcggca
      601 gcgcgtgaag ctcgcgatgg cgatcgctca tgaccccgac ctcgtggtgc tcgacgagcc
      661 gaccgacggc ctcgacccga cccagcgcga ggcaatgctg tcgctcattc gccgcctcgc
      721 cgtcgagttc aacatgagcg tgttgttgag ttcgcatctg ctcgacgagg tcgagcgcac
      781 gtgtgatcgc gtcgtgatcc tcaacggcgg ccgggtcacg gcatcggggg aactctcggc
      841 gctgaaaggc gagggatccg gcgtaagcgt cgagttgctc gagggcgcgg aatcgttcgc
      901 ccggcgcatg agcgctcggg gattcgaggt gagctgcgac gggccactcg tggtcacccg
      961 ctcggggtcc tcgggtgcga gccagcacga tctccagcgg atcatccgcg acgac
//
LOCUS       DAOOMA010000707         1009 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_207587_length_1009_cov_4.953878, whole genome shotgun
            sequence.
ACCESSION   DAOOMA010000707 DAOOMA010000000
VERSION     DAOOMA010000707.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 1009)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1009)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1009
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_207587_length_1009_cov_4.953878"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..598)
                     /locus_tag="PLP95_14055"
     CDS             complement(<1..598)
                     /locus_tag="PLP95_14055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB46971.1"
                     /translation="MARRRTPEHPTHLRSAAMVDIRSGELVEPGSLLIIDDRIAEVSP
                     TALPDGTVTIDLGDVTLLPGLMDMEVNLFLGGPDHASPLTAVQEDPATRTLRGVVNGK
                     RTLRAGFTTVRNLGLFIQTNGILLDVALAKAIDRGWFEGPRVVSAGHAICPTGGHLDP
                     TMFQAFAPHVLPLTVEEGIANGIDEVRKAVRYQIKHGAQ"
     gene            complement(653..>1009)
                     /locus_tag="PLP95_14060"
     CDS             complement(653..>1009)
                     /locus_tag="PLP95_14060"
                     /inference="COORDINATES: protein motif:HMM:NF012296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HPB46972.1"
                     /translation="RYEAPSPVNARWVTRDVDHHGQTLPADSIVLLLNGSANRDERRF
                     PDADRFDVRRDIGHHFAFGYGIHFCLGAALARLEGRVALDEVLKRFPTWEVDWDNAVQ
                     ARTSTVRGWEKLPVTF"
BASE COUNT          158 a          293 c          381 g          177 t
ORIGIN      
        1 cttgagcccc gtgcttgatc tgatagcgca ctgccttgcg gacctcatcg atgccgttgg
       61 cgatgccctc ctcgacggtg aggggcaaca cgtgcggggc gaatgcctgg aacatcgtcg
      121 ggtcgaggtg tcccccggtc gggcagatgg cgtgtcctgc cgacacgacg cggggcccct
      181 cgaaccaccc ccggtcgatg gccttcgcca acgcgacgtc caacaggatc ccgttggtct
      241 ggatgaacag tcccagattg cgcacggtgg tgaacccggc gcgcagtgtg cgtttgccgt
      301 tgacgacgcc gcgaagtgtc cgggtcgccg ggtcttcctg tacggcggtg agggggctgg
      361 cgtgatccgg gccgccgaga aagaggttga cctccatatc catgaggccg ggcaacaagg
      421 tgacgtcgcc gaggtcgatg gtgacggtgc cgtcgggcag ggccgtgggg gagacctcgg
      481 cgatgcggtc gtcgatgatg agcagcgagc cgggttcgac gagttccccc gatctgatgt
      541 cgaccatggc agcgctgcgc agatgggtcg gatgctcggg tgtgcgacgt cgcgccacgg
      601 tgatgctcct gaacgggtcg tgaggtgtga aaactcggtt gggtgctggc ggttagaagg
      661 tgaccgggag cttctcccag cctcgaacgg tggaggtgcg ggcctgcacg gcgttgtccc
      721 aatcgacctc ccaggtgggg aatcgcttga ggacctcgtc gagggcgacc cgtccttcga
      781 ggcgggcgag ggcggctccg aggcagaagt ggatgccgta gccgaaggcg aagtggtggc
      841 cgatgtcgcg gcgcacatcg aaccgatcgg cgtcggggaa ccggcgctcg tcgcggttgg
      901 ccgagccgtt caacaacaac acgatgctgt cggcgggaag cgtctggccg tggtggtcga
      961 cgtctcgggt cacccaccgg gcattgaccg gcgatggggc ttcgtagcg
//
LOCUS       DAOOMA010000708         1006 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_2687
            NODE_208693_length_1006_cov_2.879075, whole genome shotgun
            sequence.
ACCESSION   DAOOMA010000708 DAOOMA010000000
VERSION     DAOOMA010000708.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864762
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 1006)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1006)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:17:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,814
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 14 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1006
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_208693_length_1006_cov_2.879075"
                     /isolate="AMR_MDS_2687"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..414)
                     /locus_tag="PLP95_14065"
     CDS             complement(<1..414)
                     /locus_tag="PLP95_14065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB46973.1"
                     /translation="MASPTVFSRSRRFSSVLLPPLVALGLLGSGCTSSDNGSGGGSAD
                     TTTTTAVPNPCTPQGDETMAKALVRCGDASIAYLSTEDVSGTGIVIERDSKRYVLTNA
                     HVIDPYDSADVILAGETYDAMEVVGVEQFGGKPEDL"
     gene            complement(461..>1006)
                     /locus_tag="PLP95_14070"
     CDS             complement(461..>1006)
                     /locus_tag="PLP95_14070"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HPB46974.1"
                     /translation="DIEVGRDIEQSYLALGWRGIVATDPQRVALAVLNHALGDGPSSR
                     LHEEIRERRGLAYGVSSSAASNLDAGTQSVFVATAPEHLGEVRRIIDDEIARVLDEGI
                     DDEELEVAKGYLTGSMLMALEDSSSRMSRLGSAVQTHGRILKVSESLDAIDAVTGEDV
                     RSVAARIFGAPRVTSLVGPQG"
BASE COUNT          173 a          350 c          315 g          168 t
ORIGIN      
        1 gagatcctcg ggtttcccgc cgaactgttc gaccccgacg acctccatcg cgtcgtaggt
       61 ctcgccggcc agaatcacgt ccgccgagtc gtaggggtcg atcacatggg cgttggtgag
      121 cacatatcgc tttgagtcgc gttcgatgac gatgcccgtg cccgacacgt cctcggtgga
      181 cagatacgcg atcgacgcgt ccccgcaccg cacgagcgcc ttggccatcg tctcgtcccc
      241 ctgcggggtg cagggattgg gcaccgccgt ggtcgtcgtc gtgtccgcgg aaccgccacc
      301 actgccgttg tcggaggacg tacagccgct gcccaacagg cccagcgcca ccaggggcgg
      361 caacaaaacc gacgagaaac gtcgagaacg cgaaaagacc gtcggggaag ccatatccgg
      421 ctcatcgtat cgggctgaat gagcgttcag ttctcgccga tcaaccttgt ggaccgacca
      481 gggatgtgac acgtggcgca ccgaagatcc gggcggccac ggagcgcaca tcctcgccgg
      541 ttacggcgtc gatcgcgtcg agcgactcgc tcaccttgag gatgcggcca tgggtctgca
      601 cggccgagcc gagccggctc atccgcgacg aggagtcctc gagcgccatc agcatcgatc
      661 cggtgaggta gcccttcgcg acctcgagtt cctcgtcgtc gatcccctcg tcgagcaccc
      721 gtgcgatctc gtcgtcgatg atgcgccgga cctcgccgag gtgttcgggg gccgtcgcga
      781 caaacacgct ctgggttccg gcatcgaggt tgctcgccgc cgaggaggac accccatagg
      841 ccagcccacg ccgttcgcgg atctcctcgt gcagccgact cgacggcccg tctccgagcg
      901 cgtgattcaa taccgccaac gccacacgct gaggatcggt cgccacgatg ccgcgccagc
      961 ccagggcgag atagctctgc tcgatgtcgc gcccgacctc gatgtc
//
